Podcasts about contractile

In this episode, we dive into the incredible role of T4D-Myoviridae bacteriophages in targeting Escherichia coli strains, highlighting their unique contractile tail structures that facilitate precise DNA injection into host cells. We explore the specific interactions between phage tail fibers and bacterial lipopolysaccharide receptors, illustrating the phage's lock-and-key mechanism crucial for selective bacterial lysis. This discussion extends into the implications for combatting SIBO and enhancing gut microbiota balance while exploring the impressive scientific literature on phage-mediated modulation of gut flora and inflammatory markers. Topics: 1. Introduction to Bacteriophages - Explanation of what a bacteriophage (phage) is. - Importance of phages in research and their role in intestinal dysbiosis. 2. Bacteriophage Foundation - Description of bacteriophage structure: - Capsid and its genetic material (DNA or RNA). - Icosahedral head and its efficiency. - Types of phage tails and their functions: - Contractile tails: mechanism similar to a syringe. - Non-contractile tails: static structure for attachment. 3. Phage-Bacteria Interaction - Initial attachment to bacterial cell via tail fibers. - Specificity of attachment (lock and key mechanism). - Injection of genetic material into the bacterial cell. - Use of bacterial machinery to replicate phage components. 4. Phage Replication Cycle - Overview of the phage replication process within the host cell: - Integration and takeover of host cellular machinery. - Assembly of new phage particles. - Release of phages through lysis of the host cell. 5. Application - Potential of phages to selectively target and kill bacteria. - Benefits over broad-spectrum targeting. - Discussion of antibiotic resistance. 6. Diving into the Gut Microbiome - Connection between phages and the gut microbiome. - Discussion of specific phages like T4D-Myoviridae and their targets. - Relevance to SIBO (Small Intestinal Bacterial Overgrowth). 7. Case Studies and Research - Review of studies on phage impact on gut microbiota and inflammation. - Specific changes in microbiota observed in research (e.g., increase in butyrate-producing genera). Thank you to our episode sponsor: ⁠⁠⁠⁠⁠⁠Liver Medic⁠⁠⁠⁠⁠⁠⁠ Use code Chloe20 to save 20% on ⁠⁠⁠⁠⁠⁠⁠⁠"Leaky Gut Repair"⁠⁠⁠⁠⁠⁠⁠⁠ ⁠⁠⁠⁠⁠⁠⁠⁠Brendan's YouTube Channel⁠⁠⁠⁠⁠⁠⁠⁠ ⁠⁠⁠⁠⁠⁠⁠⁠https://x.com/livermedic⁠⁠⁠⁠⁠⁠⁠⁠ Thanks for tuning in! Get Chloe's Book Today! "⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠75 Gut-Healing Strategies & Biohacks⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠" Follow Chloe on Instagram ⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠@synthesisofwellness⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠ Follow Chloe on TikTok @chloe_c_porter Visit ⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠synthesisofwellness.com⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠ to purchase products, subscribe to our mailing list, and more! --- Support this podcast: https://podcasters.spotify.com/pod/show/chloe-porter6/support

How to know when to take more rest days, Arm Training Critique & more TIME STAMPS BELOW BSG Coaching QA Skip Hill, Andrew Berry, Scott McNally & Ft @NastyNateSpear

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2023.07.25.550562v1?rss=1 Authors: Phillips, J. E., Pan, D. Abstract: The genomes of close unicellular relatives of animals encode orthologs of many genes that regulate animal development. However, little is known about the function of such genes in unicellular organisms or the evolutionary process by which these genes came to function in multicellular development. The Hippo pathway, which regulates cell proliferation and tissue size in animals, is present in some of the closest unicellular relatives of animals, including the amoeboid organism Capsaspora owczarzaki. We previously showed that the Capsaspora ortholog of the Hippo pathway nuclear effector Yorkie/YAP/TAZ (coYki) regulates actin dynamics and the three-dimensional morphology of Capsaspora cell aggregates, but is dispensable for cell proliferation control (Phillips et al., 2022). However, the function of upstream Hippo pathway components, and whether and how they regulate coYki in Capsaspora, remained unknown. Here, we analyze the function of the upstream Hippo pathway kinases coHpo and coWts in Capsaspora by generating mutant lines for each gene. Loss of either kinase results in increased nuclear localization of coYki, indicating an ancient, premetazoan origin of this Hippo pathway regulatory mechanism. Strikingly, we find that loss of either kinase causes a contractile cell behavior and increased density of cell packing within Capsaspora aggregates. We further show that this increased cell density is not due to differences in proliferation, but rather actomyosin-dependent changes in the multicellular architecture of aggregates. Given its well-established role in cell density-regulated proliferation in animals, the increased density of cell packing in coHpo and coWts mutants suggests a shared and possibly ancient and conserved function of the Hippo pathway in cell density control. Together, these results implicate cytoskeletal regulation but not proliferation as an ancestral function of the Hippo pathway and uncover a novel role for Hippo signaling in regulating cell density in a proliferation-independent manner. Copy rights belong to original authors. Visit the link for more info Podcast created by Paper Player, LLC

Skeletal Muscle is Elastic, Excitable and Contractile. Does Electric Stimulation Acupuncture at the Motor Points of the muscle restore those qualities after injury has made the muscle short, weak and inhibited?   The German word for "numb" is "taub". I think muscle inhibition may be due to the muscle being deaf, not listening to the nerve signals. What if it's not the nerve, but the muscle that is causing muscle inhibition?

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2023.03.01.530611v1?rss=1 Authors: Bellingham-Johnstun, K., Tyree, Z. L., Martinez-Baird, J., Thorn, A., Laplante, C. Abstract: Actin-microtubule interactions are critical for cell division yet how these networks of polymers mutually influence their mechanical properties and functions in live cells remains unknown. In fission yeast, the post-anaphase array (PAA) of microtubules assembles in the plane of the contractile ring and its assembly relies on the Myp2p-dependent recruitment of Mto1p, a component of equatorial microtubule organizing centers (eMTOCs). The general organization of this array of microtubule and the impact on their physical attachment to the contractile ring remain unclear. We found that Myp2p facilitates the recruitment of Mto1p to the inner face of the contractile ring where the eMTOCs polymerize microtubules without their direct interaction. The PAA microtubules form a dynamic polygon of Ase1p crosslinked microtubules inside the contractile ring. The specific loss of PAA microtubules affects the mechanical properties of the contractile ring of actin by lowering its stiffness. This change in the mechanical properties of the ring has no measurable impact on cytokinesis or on the anchoring of the ring. Our work proposes that the PAA microtubules exploit the contractile ring for their assembly and function during cell division while the contractile ring may receive no benefit from these interactions. Copy rights belong to original authors. Visit the link for more info Podcast created by Paper Player, LLC

There are two types of tendon dysfunction - tendonitis (reactive inflammation due to overloading) and tendinopathy (chronic tissue deformation due to chronic overload and no remodeling program). Recovery from these are not always straightforward. Generally, people tend to do “too much too soon after doing too little for too long”. That results in delayed healing time and continued inflammation. The inflammatory phase in a tissue injury is typically 10 days and during that time we can take some steps to improve our recovery. The acronym RICE which stands for Rest, Ice, Compression, Elevation is no longer applicable and instead, a new acronym called PEACE & LOVE is being applied with greater results. We discuss all that and more today! We discuss: The most common regions of injury including the Achilles, patellar tendon, plantar fascia, rotator cuff (supraspinatus), and wrist extensors (tennis elbow). Generally how people do “too much too soon after doing too little for too long”, and how that can be more harmful than beneficial. Two types of tendon dysfunction – tendonitis (reactive inflammation due to overloading) and tendinopathy (chronic tissue deformation due to chronic overload and no remodeling program) How the acronym PEACE & LOVE has superseded RICE in the preferred way to heal these injuries. What are Isometric, Eccentric, and Concentric contractions, and what are examples of each? Guest Bio: Dr. Courtney Clark has a doctorate in Physical therapy and she is a board-certified orthopedic clinical specialist. She is also the clinic director at East riverside results PT. She has a great passion for orthopedic care and in-depth knowledge of the science of pain and pain management. She uses an individualized approach to each patient. She lives in south Austin and enjoys a variety of outdoor activities. Connect with Dr. Clark Website: https://www.resultspt.com/east-riverside-austin-texas LinkedIn: https://www.linkedin.com/in/dr-courtney-clark-692577a1/ Connect with Dr. Akhter Website: www.madinamedicine.com Email: info@madinamedicine.com Instagram: www.instagram.com/dromarakhter LinkedIn: https://www.linkedin.com/in/dromarakhter/

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2020.10.28.358663v1?rss=1 Authors: Balasubramaniam, L., Doostmohammadi, A., Saw, T. B., Sankara, G., Mueller, R., Dang, T., Thomas, M., Gupta, S., Sonam, S., Yap, A. S., Toyama, Y., Mege, R.-M., Yeomans, J., Ladoux, B. Abstract: Actomyosin machinery endows cells with contractility at a single cell level. However, at a tissue scale, cells can show either contractile or extensile behaviour based on the direction of pushing or pulling forces due to neighbour interactions or substrate interactions. Previous studies have shown that a monolayer of fibroblasts behaves as a contractile system while a monolayer of epithelial cells or neural crest cells behaves as an extensile system. How these two contradictory sources of force generation can coexist has remained unexplained. Through a combination of experiments using MDCK (Madin Darby Canine Kidney) cells, and in-silico modeling, we uncover the mechanism behind this switch in behaviour of epithelial cell monolayers from extensile to contractile as the weakening of intercellular contacts. We find that this switch in active behaviour also promotes the buildup of tension at the cell substrate interface through an increase in actin stress fibers and higher traction forces. This in turn triggers a mechanotransductive response in vinculin translocation to focal adhesion sites and YAP (Yes-associated protein) transcription factor activation. Our studies also show that differences in extensility and contractility act to sort cells, thus determining a general mechanism for mechanobiological pattern formation during cell competition, morphogenesis and cancer progression. Copy rights belong to original authors. Visit the link for more info

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2020.08.22.262758v1?rss=1 Authors: Liu, K., Patteson, A. E., Banigan, E. J., Schwarz, J. M. Abstract: The cell nucleus houses the chromosomes, which are linked to a soft shell of lamin filaments. Experiments indicate that correlated chromosome dynamics and nuclear shape fluctuations arise from motor activity. To identify the physical mechanisms, we develop a model of an active, crosslinked Rouse chain bound to a polymeric shell. System-sized correlated motions occur but require both motor activity and crosslinks. Contractile motors, in particular, enhance chromosome dynamics by driving anomalous density fluctuations. Nuclear shape fluctuations depend on motor strength, crosslinking, and chromosome-lamina binding. Therefore, complex chromatin dynamics and nuclear shape emerge from a minimal, active chromosome-lamina system. Copy rights belong to original authors. Visit the link for more info

In this episode Peter & Alex sit down with Anthony Raccomandato. Anthony is the creator of Eldoa Rx. Anthony has a backround in exercise science and is a student of French Osteopath Guy Voyer. This episode we discuss: The Eldoa, what is it & how is it used. Anthony tells us about his view of the body, the Biotensegrity approach. We talk Fascia, what is it & how does it differ from muscle tissue? Foam rolling & its effects on fascia. Contractile components of Fascia & how they relate to reactivity & pain. And much much more! You can Find out more about Anthony at https://www.eldoarx.com/about-eldoa

Phillip Beach is an Osteopath and Traditional Chinese Medicine (TCM) researcher. He runs a private practice in Wellington, New Zealand. Phillip is interested in developing models of human movement that are congruent with embryology and evolutionary biology. Phillip is the author of ‘Muscles and Meridians: The Manipulation of Shape’ He lived in Malaysia, Papua New Guinea and Nigeria as a child and left Sydney to study in London Phillip has an interest in Thai Massage, Tui Na and works primarily on the floor rather than a treatment table. He uses his feet a lot in his work Evolutionary biology: we are apes and have a 30 million year history, so it makes sense to study this background He originally taught what he was taught and then developed his own ideas about contractile fields. These were originally similar to Tom Myers’ work on muscle chains Looking at embryology and evolutionary biology He developed his work further by looking at whole fields of movement, not just how one muscle joins another muscle Exploring field theory. Each field goes from the facial region down to the pelvic floor Fields: forwards and backwards, left and right The model has been developed following years of exploration and discovery Phillip teaches osteopaths, pilates teachers and other bodyworkers or therapists that work with the ease and dis-ease of human movement Archetypal postures - the necessity of retiring to the floor to rest: cross-legged, squatting and Japanese posture (sitting on the heels) http://phillipbeach.com/archetypal-postures/ The relationship between work (movement) and rest Sitting cross-legged on the floor helps the venous drainage of the legs Squatting fully with the heels flat allows a traction and flexion of the lumbar spine  For the human physique, the full squat and sitting cross-legged are the ‘middle C’ of our biomechanical resting lives To practice squatting, stand in a door-frame and drop down as far as you can, with the knees wide. Lift the heels if needed. As the achilles elongate, the full squat becomes easier. To squat well, a full range of dorsiflexion of the ankle is needed. Premature babies tend to walk on tiptoes as they haven’t had full dorsi-flexion in utero To improve sitting cross-legged, use a meditation pillow, keep the legs wide. Some people that can sit easily in the cross-legged position don’t like to squat and vice versa The feet in the shoes are ‘sensory deprivation chambers’ The fine tuning from using the feet properly is essential for walking and running Phillip turned his kitchen floor into a ‘rock garden’ with different shaped rock surfaces to bring the outside inside and changed the body system Your physique wants movement, and access to the floor Limber - floor to sitting to standing desk in New Zealand: https://limber.nz/ Micro-movements keep you alive.  Make your office ‘unergonomic’. Keep your phone and water away from you. Don’t have wheels on your chair. Don’t make your chair too comfortable. Make your life a little more difficult so that you have to move Place a value on floor life Hip and knee replacements are becoming so expensive. Floor life for appropriate rest is needed If you want to age well, spend more time on the floor The floor is the passive aspect. The active side is standing up from the floor Standing up started the cascade of us becoming the dominant species on the planet Style, power and poise to stand from the floor - ‘erectorcises’  Children need to be barefoot as much as possible on rough ground, spending as much time on the floor Sleeping on the ground is important so the first thing you do is stand from the floor ‘Soft bodies need hard beds and hard bodies need soft beds’ Brachiation - swinging and hanging in apes The human pelvic floor is thicker than other mammals for the upright position It’s possible to carry 40% of your body-weight on your head   Resources: Website for information about Phillip’s courses and workshops: www.phillipbeach.com Book: Muscles and Meridians: The Manipulation of Shape by Phillip Beach https://www.amazon.co.uk/gp/product/0702031097/ref=as_li_qf_asin_il_tl?ie=UTF8&tag=avnitouch-21&creative=6738&linkCode=as2&creativeASIN=0702031097&linkId=ca632c577c26e9c460eadebad12033a0

Phill Beach an osteopath, acupuncturist and truly innovative thinker leads listeners through his deep evolutionary and embryological insights which led him to develop his fascinating and clinically applied concepts of Contractile Fields, Archetypal Rest Postures, Decoding the Meridial Map and the importance of brachiation among many other gems. Phill is a unique thinker, and this podcast with him is a rare gem allowing you to get a glimpse inside the mind of someone who takes biological wisdom to another level.

Episode 39 of Uncharted performance brings you Joel Woods, creator of CLE Muscle Lab. His approach to fixing bad movement patterns, strengthening weaknesses and simply helping people of all levels of fitness move better is called Muscle Activation Technique (MAT) and its pretty refreshing in the world of same old tired physical therapy routines. We’ll discuss how MAT works, in what order we should progress to avoid injury and develop optimal “fitness”, as well as how to keep your body physically resilient well into old age while still having fun and being active. Joel wants us all to stay strong and be able to exercise for life, not the other way around. Hope you enjoy this episode as much as we did!

Hosts: Vincent Racaniello and Elio Schaechter. Vincent and Elio marvel in the finding that a phage tail-like structure from a marine bacterium stimulates tubeworm metamorphosis, and reveal Ophidiomyces as a cause of snake fungal disease. Subscribe to TWiM (free) on iTunes, Stitcher, Android, RSS, or by email. You can also listen on your mobile device with the Microbeworld app. Links for this episode  Tubeworm metamorphosis by phage tail-like structures (Science) Cell enzyme becomes viral capsid protein (virology blog) Snake fungal disease in the laboratory (mBioi) Snake fungal disease in cottonmouths (PLoS Biol) Snake fungal disease (pdf) Image credit This episode is sponsored by ASM Grant Writing Institute Online Webinar and 32nd Clinical Virology Symposium Music used on TWiM is composed and performed by Ronald Jenkees and used with permission. Send your microbiology questions and comments (email or mp3 file) to twim@twiv.tv.  

Commentary by Dr. Valentin Fuster

Margaret Gardel explains the steps involved in building a contractile bundle for cell adhesion: actomyosin remodeling, F-actin network contraction and stress fiber formation.