Podcasts about transglutaminase

Food and Travel Nation with Elizabeth Dougherty THAT TASTY FILET MIGHT CONTAIN A NASTY SECRET The TRUTH about Food and Travel Original Release Date:  01/14/2015 EPISODE 1642 Before you order that expensive filet, listen to our interview on "meat glue" because it's back in the news.  From the Food And Travel Nation archives, Elizabeth Dougherty spoke with attorney Bill Marler a couple of years ago about "meat glue" and why there are possible safety concerns associated with consuming it. Transglutaminase is used to "glue" together beef, chicken, pork or fish transforming it into what appears to be one, seamless filet, steak or piece of surimi (imitation crab). It is difficult for even experts in the food industry to tell by appearance only if meat glue was used to stick protein products together. He says the government considers transglutaminase aka "meat glue" as a GRAS food additive. GRAS stands for "generally recognized as safe". We explored the meaning of that term and asked important food safety questions that challenge the GRAS designation of this food additive. Find out some of the products in the food supply "meat glue" is added to and why we think there are safety concerns about its use in our broadcast interview with Attorney Marler here: Am I eating "meat glue"? ### Each week our listeners get the very latest food and travel information We produce homemade videos of healthy, easy to make recipes We feature no-holds barred interviews in a LIVE, fast-paced, nationwide call-in show. Elizabeth Dougherty is a writer, trained chef, world traveler and now an award-winning talk show host. Food And Travel Nation was on the forefront of presenting expert guests with vital information about GMOs, at a time when no one was talking about or even knew about the subject. We give our listeners, advertisers and stations a LIVE SHOW. (NO “BEST OF'S” EVER!) We present hard-hitting topics and interviews without the same old political spin. We are very social media conscious and stay in touch with our audience. (200,000 plus) We work closely with advertisers and stations to ensure their success. We are ready to deliver a fresh, tight, first-class show to your station from our digital studio utilizing Comrex Access and our own automation system. FOOD AND TRAVEL NATION FAST FACTS Website: Food And Travel Nation.com Social Media Sites: Facebook | Twitter website:  FoodAndTravelNation.com email:  FoodTravelUSA@proton.me Executive Producer – Michael Serio

Solução produzida pela Ajinomoto do Brasil, conhecia como ACTIVA BFB, faz parte da lista dos ingredientes aprovados em nova regulamentação. Saiba mais em https://www.ajinomotofi.com.br/ --- Send in a voice message: https://podcasters.spotify.com/pod/show/megavarejo/message

Transglutaminase, also called "meat glue" is a common additive used in many foods because it works to bind protein molecules from various different substances so they ""stick" and hold together. Listen in this week as Dee explains how transglutaminase works in foods, the types of foods it typically shows up in, and whether or not it's safe to eat.

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2023.02.06.527263v1?rss=1 Authors: Emerson, J., Delgado, T., Girardi, P., Johnson, G. V. Abstract: AAstrocytes are the primary support cells of the central nervous system (CNS) that help maintain the energetic requirements and homeostatic environment of neurons. CNS injury causes astrocytes to take on reactive phenotypes with altered overall function that can range from supportive to harmful for recovering neurons. The characterization of reactive astrocyte populations is a rapidly developing field, and the underlying factors and signaling pathways governing which type of reactive phenotype that astrocytes take on is poorly understood. Our previous studies suggest that transglutaminase 2 (TG2) has an important role in determining the astrocytic response to injury. TG2 is upregulated in astrocytes across multiple injury models, and selectively deleting TG2 from astrocytes improves functional outcomes after CNS injury and causes widespread changes in gene regulation, which is associated with its nuclear localization. The underlying molecular mechanisms by which TG2 causes these functional changes are unknown, and its interactions in the nucleus of astrocytes has not yet been described. To begin to understand how TG2 impacts astrocytic function, we used a neuron-astrocyte co-culture paradigm to compare the effects of TG2-/- and wild type (WT) astrocytes on neurite outgrowth and synapse formation. We assayed neurons on both a growth-supportive substrate and an injury-simulating matrix comprised of inhibitory chondroitin sulfate proteoglycans (CSPGs). Compared to WT astrocytes, TG2-/- astrocytes supported neurite outgrowth to a significantly greater extent only on the CSPG matrix, while synapse formation assays showed mixed results depending on the pre- and post-synaptic markers analyzed. We hypothesize that TG2 regulates the supportive functions of astrocytes in injury conditions by modulating the expression of a wide range of genes through interactions with transcription factors and transcription complexes. Based on results of a previous yeast two-hybrid screen for TG2 interactors, we further investigated the interaction of TG2 with Zbtb7a, a ubiquitously expressed transcription factor. Co-immunoprecipitation and colocalization analyses confirmed the interaction of TG2 and Zbtb7a in the nucleus of astrocytes. Genetic overexpression or knockdown of Zbtb7a levels in TG2-/- and WT astrocytes revealed that Zbtb7a robustly influenced astrocytic morphology and the ability of astrocytes to support neuronal outgrowth, which was significantly modulated by the presence of TG2. These findings support our hypothesis that astrocytic TG2 acts as a transcriptional regulator to influence astrocytic function, with greater influence under injury conditions that increase its expression, and Zbtb7a likely contributes to the overall effects observed with astrocytic TG2 deletion. Copy rights belong to original authors. Visit the link for more info Podcast created by Paper Player, LLC

This is a PSA on Transglutaminase, also known as “Meat Glue”.

Doutor Rafael Lages discorrendo sobre o ensaio randomizado de inibidor da transglutaminase 2 para doença celíaca. Ref: Schuppan, D. et al. A Randomized Trial of a Transglutaminase 2 Inhibitor for Celiac Disease. New Engl J Med385, 35–45 (2021). doi:10.1056/nejmoa2032441 --- Send in a voice message: https://anchor.fm/gastrocast/message

Talk to a Dr. Berg Keto Consultant today and get the help you need on your journey (free consultation). Call 1-540-299-1557 with your questions about Keto, Intermittent Fasting, or the use of Dr. Berg products. Consultants are available Monday through Friday from 8 AM to 10 PM EST. Saturday & Sunday from 9 AM to 6 PM EST. USA Only. Get Dr. Berg's Veggie Solution today! • Flavored (Sweetened) - https://shop.drberg.com/veggie-solution-flavored-sweetened?utm_source=Podcast&utm_medium=AGM(Anchor) • Plain (Unflavored) - https://shop.drberg.com/veggie-solution-plain?utm_source=Podcast&utm_medium=AGM(Anchor) Take Dr. Berg's Free Keto Mini-Course! In this podcast, Dr. Berg talks about chicken nugget. Here's what this food consists of, 1. Chicken – Feed GMO 2. Food Starch Modified 3. Salt 4. Wheat Starch 5. Flavoring 6. Soy oil 7. Wheat Starch 8. Bleached Flour 9. Reduced Iron 10. Corn Flour 11. Baking Soda 12. Sodium Aluminum Phosphate 13. TBHQ 14. Transglutaminase – it is a type of meat glue that mimics gluten in the body which could cause allergy, sensitivity and can trigger a condition called celiac disease. Iron Toxicity: http://bit.ly/2HXMQ61 Dr. Eric Berg DC Bio: Dr. Berg, 51 years of age is a chiropractor who specializes in weight loss through nutritional & natural methods. His private practice is located in Alexandria, Virginia. His clients include senior officials in the U.S. government & the Justice Department, ambassadors, medical doctors, high-level executives of prominent corporations, scientists, engineers, professors, and other clients from all walks of life. He is the author of The 7 Principles of Fat Burning. FACEBOOK: fb.me/DrEricBerg?utm_source=Podcast&utm_medium=Anchor TWITTER: http://twitter.com/DrBergDC?utm_source=Podcast&utm_medium=Post&utm_campaign=Daily%20Post YOUTUBE: http://www.youtube.com/user/drericberg123?utm_source=Podcast&utm_medium=Anchor DR. BERG'S SHOP: https://shop.drberg.com/?utm_source=Podcast&utm_medium=Anchor MESSENGER: https://www.messenger.com/t/drericberg?utm_source=Podcast&utm_medium=Anchor DR. BERG'S VIDEO BLOG: https://www.drberg.com/blog?utm_source=Podcast&utm_medium=Anchor

Talk to a Dr. Berg Keto Consultant today and get the help you need on your journey (free consultation). Call 1-540-299-1557 with your questions about Keto, Intermittent Fasting or the use of Dr. Berg products. Consultants are available Monday through Friday from 8:30 am to 9 pm EST. Saturday & Sunday 9 am to 5 pm EST. USA Only. Take Dr. Berg's Free Keto Mini-Course! If you feel like you can't live without carbs, this podcast is for you. Can't live without bread? Did you know this: • The average American consumes 53 pounds (26 kg) of bread per year. • The glycemic index of bread is 71 - 81. • Low-fat bread has too many carbs that turn into fat and then spikes insulin, which blocks the ability to burn the fat. • A lot of white bread has bleach (chlorine dioxide) in it, which may lead to type 1 diabetes. • Transglutaminase is an enzyme that can worsen the sensitivity to gluten. • When you consume whole grains, you're getting bran, which is loaded with phytic acid that blocks zinc. • The more bread you consume, the more you're deleted of B-vitamins, vitamin E, and zinc. Vitamin B1 and vitamin E are essential for the heart. Keto-friendly alternatives to bread: 1. Oopsie bread (cloud bread) 2. You can make bread out of almond flour, cauliflower, chia seed, flaxseed, coconut flour, and zucchini 3. My keto-friendly bread recipe If you're craving bread, you're usually deficient in vitamin B1. Taking nutritional yeast can help reduce these cravings. Healthy keto and intermittent fasting can also help make your hunger go down, making it easier to avoid bread. Dr. Eric Berg DC Bio: Dr. Berg, 51 years of age is a chiropractor who specializes in weight loss through nutritional & natural methods. His private practice is located in Alexandria, Virginia. His clients include senior officials in the U.S. government & the Justice Department, ambassadors, medical doctors, high-level executives of prominent corporations, scientists, engineers, professors, and other clients from all walks of life. He is the author of The 7 Principles of Fat Burning. FACEBOOK: fb.me/DrEricBerg?utm_source=Podcast&utm_medium=Anchor TWITTER: http://twitter.com/DrBergDC?utm_source=Podcast&utm_medium=Post&utm_campaign=Daily%20Post YOUTUBE: http://www.youtube.com/user/drericberg123?utm_source=Podcast&utm_medium=Anchor DR. BERG'S SHOP: https://shop.drberg.com/?utm_source=Podcast&utm_medium=Anchor MESSENGER: https://www.messenger.com/t/drericberg?utm_source=Podcast&utm_medium=Anchor DR. BERG'S VIDEO BLOG: https://www.drberg.com/blog?utm_source=Podcast&utm_medium=Anchor

Dr. Pastore covers a well-known, yet not well-understood diagnostic marker of celiac disease -  tissue transglutaminase (TTG). He covers the function TTG plays as an enzyme, how TTG affects gluten and shares vital information about various TTG blood tests (TTG IgA and TTG IgG) for those looking for a diagnosis, and for the diagnosed celiac wanting to monitor their progress. Show notes: https://drrobertpastore.com/podcasts/063-tissue-transglutaminase

INGREDIENTS OF THE DAY:  Methyl Cellulose, Glutamic Acid, Agar, Cultured Sugar, Black Sea Salt, Mung Bean Protein Isolate, Gellan Gum, Transglutaminase, Nisin, Brown Rice Flour, White Whole Grain Sorghum Flour, Milled Flax Seed In this episode, which will probably be the last of the diet programs, I describe processed foods found in the plant-based diet, a food regimen which is paralleling the other diets in terms of popularity and food choices.  Topics will include a little history of the diet, what it includes and excludes, and a short exploration of a few commercial foods tailored to plant-based eaters.  Along the way, I come across some new and unusual ingredients and additives.  Show Notes:  To Contact Show:  foodlabelsrevealed@gmail.com  Facebook Page:  https://www.facebook.com/prophetofprocessedfood/?ref=bookmarks The podcast can be subscribed to at the iTunes store or using most of the podcast apps available for smart phones or tablets.  Just search under Food Labels Revealed.   References: Morningstar Foods https://www.morningstarfarms.com/products.html Sweet Earth Foods https://www.sweetearthfoods.com/ Daiya Foods https://daiyafoods.com/ Miyoko’s Creamery https://miyokos.com/ Vegg Foods https://thevegg.com/ Just Foods https://www.ju.st/en-us/products/consumer/egg/egg Ben & Jerry’s Non-Dairy Ice Cream https://www.benjerry.com/flavors/non-dairy Meat Glue http://www.cookingissues.com/transglutaminase-aka-meat-glue/index.html Entry on Veganism - Wikipedia https://en.wikipedia.org/wiki/Veganism  Vegetarianism by Country https://en.wikipedia.org/wiki/Vegetarianism_by_country#India    Music: Intro music is the "Peter Gunn Theme" by Henri Mancini Outro music is "Derp Nugget" courtesy of Kevin MacLeod

BBQ can often be described as a dichotomy of science and art. This week Lyle and Collin discuss the scientific aspect of BBQ. From using enzymes in competition to BBQ robots and virtual Brisket. So throw on your coat and goggles and step into the BBQ lab with The Mid Mo Smoke Show

According to the University of Chicago Celiac Disease Center, 10% of MS patients also suffer from celiac disease. Studies conducted in Italy and Spain uncovered a significant increased prevalence of celiac disease in first-degree relatives of MS patients, 18% and 32% respectively. Additional research has identified situations in which celiac disease mimicked other neurological disorders such as MS and ALS.  With evidence like that, it is definitely worth it to further explore the relationship between MS and celiac disease. Today, the Gluten Free RN investigates whether MS and celiac disease are similar, related, or perhaps the same thing, as she shares case studies from the literature and her own experience. Nadine discusses the story of Dr. Terry Wahls, explaining how she was able to resolve her symptoms of MS with a variation of a Paleo diet. She outlines the analogous symptoms of MS and celiac cerebellar ataxia, the neurological damage caused by gluten, and how malabsorption of nutrients caused by intestinal damage can extend to medication. Listen and learn how Nadine and others have resolved symptoms of MS and other neurological disorders on a gluten-free diet! What’s Discussed:  Nadine’s MS scare Bouts with falling, dragging feet Numbness, tingling (neuropathy) Symptoms disappeared after adopting gluten-free diet Takes two to three years to resolve neurological symptoms Dr. Terry Wahls’ story Diagnosed with MS, confined to wheelchair Continued to research condition Adopted version of Paleo diet Now walks without cane, rides bike again Conducts seminars to share how healed mitochondria The commonalities between celiac disease and MS Both autoimmune issues Studies necessary to determine if conditions are similar, related, or the same How celiac disease affects the body Triggered by ingestion of gluten Causes villous atrophy, cryptic hyperplasia in small intestines, damage to immune system Primarily neurological disorder The symptoms of celiac cerebellar ataxia Poor balance Hard to distinguish where body is in space Trip, stumble or fall Related to vision The multi-organ manifestations of celiac disease in the central nervous system White matter lesions Inflammatory cerebral spinal fluid syndrome Sinus venous thrombosis Epilepsy, seizure disorders Cerebellar pontine atrophy Encephalopathy Myelopathy Dementia Gluten ataxia Migraines, headaches Depression, anxiety Cerebellar ataxia Nadine’s patient who wasn’t responding to MS medication Nadine recommended celiac testing Panel negative, but gene carrier Already adopted gluten-free diet Fewer medications necessary Hope to decrease, discontinue meds Malabsorption issues extend to medications, could stem from undiagnosed celiac disease The 2014 study in Vienna regarding celiac disease and MS Concluded that celiac disease may mimic MS Celiac disease may be present despite absence of anti-gliadin antibodies If patient history indicates gastrointestinal issues, celiac disease should be considered even if CSF and MRI findings suggest MS The new evidence around seronegativity in celiac disease Most labs only test for tissue transglutaminase 2 Transglutaminase 6 is specific for neurological damage caused by gluten The 2014 Italian study of the genetic overlap between celiac disease and MS Sites significant increased prevalence of celiac disease in MS first-degree relatives (18%) Recommends celiac testing in MS patients who aren’t responding to MS treatment or present with ataxia The association between MS and celiac disease as reported by the University of Chicago Celiac Disease Center Approximately 10% of those who have MS also have celiac disease The genes that indicate a predisposition to celiac disease HLA-DQ2 HLA-DQ8 The 2013 Iranian case study of a celiac patient diagnosed with MS 37-year-old woman with history of chronic abdominal pain Diagnosed with celiac disease, adopted gluten-free diet Became ataxic, met with neurologist MRI indicated multiple sclerosis Nadine argues that patient likely re-exposed to gluten How gluten acts as a neurotoxin Pastor exposed to gluten in cookies had acute neurological event Shaking, tremors (similar to stroke) The 2007 Oxford case study of celiac disease mimicking ALS 44-year-old man with progressive right-sided spastic hemiparesis (muscle wasting) Remote family history of celiac disease Strict gluten-free diet resolved most symptoms Why patients with neurological symptoms should be tested for celiac disease The anti-gliadin antibody is excellent biomarker for gluten sensitivity Why it is acceptable to adopt a gluten-free diet if your antibody test is negative for celiac disease The Paleo diet Nadine suggests for celiac and gluten-sensitive patients Gluten-, dairy-free Whole food Low carb, super-good high fat The increased prevalence of celiac disease in MS patients revealed in a 2011 study Increased prevalence found in eight of 72 MS patients (11.1%) Also in first-degree relatives (13/126 or 32%) Advises increased efforts aimed at early detection and dietary treatment The fundamentals of MS Chronic disease of unknown etiology T-cell mediated antibody response Leads to demyelination of central nervous system Associated with autoimmunity (body attacking itself) Damage to enteric nervous system can lead to constipation How to conduct your own research around celiac disease and neurological disorders Celiac researcher Dr. Marios Hadjivassiliou How long it takes to heal on a gluten-free diet Villous atrophy heals in six months to one year Neurological damage takes two to four years The nutrients a celiac patient is missing that contribute to neurological issues B vitamins Trace amounts of copper Magnesium Zinc The autoimmune symptoms Nadine resolved/reversed with a gluten-free diet MS symptoms Graves’ disease Positive ANA panel Why it’s important for celiac patients to work with educated practitioners who understand functional medicine Resources: Dr. Terry Wahls’ TED Talk The Wahls Protocol: A Radical New Way to Treat All Chronic Autoimmune Conditions Using Paleo Principle by Terry Wahls MD “Celiac Disease with Cerebral and Peripheral Nerve Involvement Mimicking Multiple Sclerosis” in the Journal of Medicine and Life “Celiac Disease in Multiple Sclerosis: A Controversial Issue” in the International Journal of Neurology and Neurotherapy University of Chicago Celiac Disease Center MS Info “Multiple Sclerosis or Neurological Manifestations of Celiac Disease” in Advanced Biomedical Research “A Case of Celiac Disease Mimicking Amyotrophic Lateral Sclerosis” in Nature Clinical Practice Neurology “Prevalence of Celiac Disease in Multiple Sclerosis” in BMC Neurology “A Case of Concurrent Multiple Sclerosis and Celiac Disease” in Govaresh “Neurological Disorders in Adult Celiac Disease” in the Canadian Journal of Gastroenterology “A Case of Multiple Sclerosis and Celiac Disease” in Case Reports in Neurological Medicine  Connect with Nadine:  Instagram Facebook Contact via Email ‘Your Skin on Gluten’ on YouTube Melodies of the Danube Gluten-Free Cruise with Nadine Books by Nadine: Dough Nation: A Nurse's Memoir of Celiac Disease from Missed Diagnosis to Food and Health Activism

Dr Tim Gerstmar practices Naturopathic Medicine at his Redmond, WA office, Aspire Natural Health. He specialises in working with people with digestive and autoimmune problems, and has worked with many of the most difficult to treat situations using a blend of natural and conventional medicine. He treats patients locally, throughout the US and as far away as the Qatar, Korea and Australia. In this interview, Dr Gerstmar discusses the tests he most commonly uses, especially for gastrointestinal complaints. We also talk about strategies for dealing with health insurance and tips for keeping costs down. These scatter plots, sometimes called calibration plots, are the ones I mentioned in the podcast. On the x-axis is what my XGBoost model predicted for previously unseen data, the y-axis represents the measured value. When the dot appears on the diagonal line, the prediction was perfect. The model was trained using results from just 260 athletes. My hope is that is these models will eventually bring down the cost of our full program by allowing us to predict the results of an expensive test using a cheaper one. Here’s the outline of this interview with Dr Tim Gerstmar: [00:00:15] First podcast: Methylation and Environmental Pollutants with Dr. Tim Gerstmar. [00:00:56] Bob McRae podcast: How to Use Biomedical Testing for IRONMAN Performance. [00:01:24] Our Elite Performance Programme. [00:04:04] How much testing should we do? [00:04:35] Factoring in lifetime costs. [00:08:33] Donating blood to the Red Cross. [00:09:44] Iron disorders: ferritin and haemochromatosis. [00:10:53] Therapeutic phlebotomy. [00:14:22] Treating symptoms is sometimes necessary. [00:15:07] Steroids for eczema. [00:17:09] Adrenal dysregulation and thyroid dysfunction. [00:18:00] You can't feel high blood sugar in diabetes. [00:18:46] AIMed conference. [00:19:06] De Fauw, Jeffrey, et al. "Automated analysis of retinal imaging using machine learning techniques for computer vision." F1000Research 5 (2016). [00:20:38] 25-OH-D testing. See Optimizing Vitamin D for Athletic Performance. [00:22:01] Insurance interfering with testing. [00:23:29] Liberty HealthShare. [00:23:42] Affordable Care Act. [00:24:10] Direct Primary Care. [00:24:23] Health Share of Oregon. [00:25:08] Covered California. [00:25:32] Health Savings Account. [00:28:52] Genova GI Effects stool test. [00:29:27] BioHealth stool test. [00:29:49] Doctor's Data stool test. [00:30:13] Coeliac diagnosis. [00:30:50] Transglutaminase. [00:31:44] Genetic risk factors. [00:32:43] NCGS and FODMAPs. [00:34:22] Intestinal lymphoma. [00:37:47] Normal test results are still useful information. [00:38:45] Liver enzymes, e.g. ALT, AST and GGT. [00:39:19] CBC and CMP, Hs-CRP. [00:39:47] Testosterone and thyroid. [00:40:09] Genova SIBO test. [00:41:08] Organic acids by Genova and Great Plains. [00:41:55] Beware insurance with OATs. [00:43:23] Verifying your policy. [00:44:07] Mitochondrial function. [00:44:21] Nutrient deficiencies. [00:44:52] Neurotransmitters and brain function. [00:45:04] Oxidative stress. [00:45:12] Detox stress, GSH status. [00:45:38] Bacterial and yeast markers. [00:46:49] Cortisol testing--DUTCH. [00:48:45] Interview with Pedro Domingos: How to Teach Machines That Can Learn. [00:49:08] XGBoost. [00:50:33] Robb Wolf early adoption costs. [00:51:54] HRV. See Elite HRV podcast. [00:52:08] Supplement companies and self-assessment questionnaires. [00:53:10] Arabinose. [00:53:48] Hallucinating from noise in the data. [00:54:52] Big Data. [00:56:03] Abnormality detection. [00:56:45] Functional versus pathological lab ranges. [00:57:46] Mark Newman. See cortisol testing above. [00:58:23] Adjusted reference ranges. [00:58:45] Vanity sizing. [01:00:52] Thyroid cancer and proximity to a mine. [01:01:21] Aspire Natural Health podcast.

Carl Franklin and Richard Morris talk to Amber O'Hearn about being a carnivore, evolution, sleep, ketogenic metabolism, and a few other tasty nuggets you won't want to miss! Errata Richard forgot to mention when working with Transglutaminase .. wear food prep gloves. Amber sent us a piece of errata. She writes: In our podcast, Carl asks me if babies *have* to be in ketosis to build brains, and I say yes, but that's not true. That is, the primary way that babies build fat and cholesterol in the brain in normal conditions *is* out of ketone bodies, though a small proportion is also made from glucose. I have a few references on this point in the talk transcript on my blog. The critical question is: What happens if there are no ketones to be had and only glucose? The answer seems to be that glucose will suffice. There is a rare inborn error of metabolism called HMG-CoA lyase deficiency, which prevents the body from making ketones. A paper by another brilliant Morris ( Cerebral ketone body metabolism ) reports that people afflicted with this have white matter abnormalities, but no noticeable loss of function, except of course, they can't go without food for long. This suggests that in cases where there are no circulating ketones, the glucose alternative pathway will take over, and get at least an adequate brain constructed. It occurs to me that children with this condition may be perfect candidates for the therapeutic use of ketones esters, provided the condition doesn't somehow prevent their use. "Given the importance of KBs [ketone bodies] as substrates for myelination, one might expect disorders of ketogenesis to be associated with cerebral white-matter abnormalities. Magnetic resonance imaging has, indeed, shown diffuse mildly increased signal in the white matter of patients with HMG-CoA lyase deficiency. Superimposed on this are foci of more abnormal signal. In most cases, multiple lesions have been present, varying in size from a few millimetres to large confluent areas; as well as in the cerebral hemispheres, they have been reported in the internal capsule and brainstem but not in the corpus callosum or cerebellar white matter. Despite the imaging abnormalities, most patients have had no neurological problems and normal or slightly below average intelligence. The findings would be compatible with hypomyelination, caused by the lack of KB."

This week on Cooking Issues, Nastassia and Jackie Molecules are on the line from D.C., while Dave talks about the closing night of Booker and Dax (while lamenting the poor quality of modern piñatas), and answers questions about vegettis, zucchini (and its sticky, slimy residue), ultrasounds, transglutaminase, nitrotinis, bark, and more!

Objective To evaluate two commercial stool tests for detection of secretory IgA antibodies against gliadin and human tissue transglutaminase for diagnosis of coeliac disease in children with symptoms.Setting Tertiary care children's hospital.Participants Coded stool samples from 20 children with newly diagnosed coeliac disease and 64 controls. Six children with coeliac disease had stool tests every two weeks for three months after starting a gluten-free diet.Main outcome measures Secretory IgA antibodies against gliadin and human tissue transglutaminase in stool samples, determined in duplicate by using recommended cut-off limits.Results Sensitivity of faecal antibodies against human tissue transglutaminase was 10% (95% confidence interval 1% to 32%), and specificity was 98% (91% to 100%). For antibodies against gliadin, sensitivity was 6% (0% to 29%) and specificity was 97% (89% to 100%). Optimisation of cut-off limits by receiver operating characteristic analysis and use of results of both tests increased sensitivity to 82%, but specificity decreased to 58%. All follow-up stool tests remained negative, except for two positive anti-gliadin results in one patient, six and 10 weeks after the gluten-free diet was started.Conclusions Neither stool test was suitable for screening for coeliac disease in children with symptoms.

Vergleich von Endomysium- und Transglutaminase-Antikörpern bei verschiedenen Patientengruppen. Verglichen wurden EmA-IgA- und TG-IgA-Antikörper-Test, wobei für den Nachweis von TG-IgA-Antikörper drei Testkits von verschiedenen Anbietern verwendet wurden. Des weiteren wurden EmA-IgG- und TG-IgG-Antikörpertests durchgeführt und die Ergebnissse miteinander verglichen. Endomysium- und TG-IgA-Antikörper-Tests zeigten vergleichbar hohe Werte für Spezifität und Sensitivität, so dass der aufwendigere Endomysium-Antikörper-Test in der Routinediagnostik durch den Transglutaminase-Test ersetzt werden kann. Der Nachweis der EmA- und TG-IgG-Antikörper ist v.a. bei häufig mit Zöliakie assoziertem Verdacht auf IgA-Mangel durchzuführen.