Podcasts about challenge test

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Best podcasts about challenge test

Latest podcast episodes about challenge test

Assurance in Action
Recycle Clearances Part 2/3: Surrogate Contaminant Test

Assurance in Action

Play Episode Listen Later Mar 7, 2024 13:57 Transcription Available


Our latest Assurance in Action podcast is the second in a three-part series that will explore recycle clearances in the United States and Europe applicable when using recycled resins in food contact applications and conducting the “the Challenge Test”. This episode focuses on the Surrogate Contaminant Test.  To learn more about “the Challenge Test”, stream our podcast now.Speakers:Mason Myers- Manager of Recycle Notification Projects, Intertek AssurisLennard (Lenny) Edwards - Project Manager, Intertek AssurisFollow us on- Intertek's Assurance In Action || Twitter || LinkedIn.

Assurance in Action
Recycle Clearances in the United States: Part 1/3

Assurance in Action

Play Episode Listen Later Feb 22, 2024 13:57 Transcription Available


FOOD CONTACT INDUSTRY SERIESOur team Assuris is excited to kick off a new podcast series! Our latest Assurance in Action podcast is the start of a three-part series that will explore recycle clearances in the United States and Europe applicable when using recycled resins in food contact applications and conducting the “the Challenge Test”. To learn more about recycle clearances, stream our podcast nowSpeakers:Mason Myers- Manager of Recycle Notification Projects, Intertek AssurisChloe Lay- Project Manager, Intertek AssurisFollow us on- Intertek's Assurance In Action || Twitter || LinkedIn.

Who Cares The Podcast
The Trivia Challenge: Test Your Knowledge for Men and Women-What's The Play

Who Cares The Podcast

Play Episode Listen Later Feb 26, 2023 26:12


The Who Cares Podcast is hosting What's The Play's Games Trivia- SUPPORT OUR SHOW ▶︎▶︎ https://www.paypal.com/donate/?hosted_button_id=CWG5C7336UM48 ▶︎▶︎https://whatstheplay.store/ SUPPORT OUR SPONSORS

81 All Out
India overcome Bangladesh's stern spin challenge: Test series review

81 All Out

Play Episode Listen Later Dec 28, 2022 57:33


In the latest episode of the podcast we discuss India's 2-0 win in the Test series in Bangladesh. Talking Points: India's close escape in the second Test in Mirpur Are Kohli and Rahul going through a bad patch or are they on the wane? Batting against spin with the new ball v old ball The challenges of playing fastish finger spin in the DRS era Axar Patel's deadly round-arm action - and why it is invaluable on these pitches Where R Ashwin stands v Kapil Dev in the allrounder stakes The non-selection of Kuldeep Yadav for the second Test - was it really shocking? Support 81allout on ko-fi Participants: Siddhartha Vaidyanathan (@sidvee) Kartikeya Date (@cricketingview) Ashoka (@ABVan) * Buy War Minus the Shooting | Buy Cricket Beyond the Bazaar Related: Is the DRS a good thing? Yes if you're a fingerspinner, no if you're a left-hand batsman - Kartikeya Date - ESPNcricinfo Training the Hawk-Eye on Axar Patel: Angular, Anomalous - Himanish Ganjoo - Substack 'Phenomenal' Shreyas Iyer calms the panic down with assured batting - Sidharth Monga - ESPNcricinfo All-rounder Ashwin, second only to Richard Hadlee, rescues India again - VS Aravind - Sportstar Mehidy Hasan Miraz and Litton Das stride towards becoming Bangladesh's next batch of match-winners - Mohammad Isam - ESPNcricinfo

Unburden Your Business
53. The Money Challenge- Test How Well You Know Your Money!

Unburden Your Business

Play Episode Listen Later Jun 27, 2022 10:07


How much does your business bring in on avg? (better yet what is the worst month you have?) How much does your business spend on avg (better yet look for the most expensive month you have had in spending) this gives you an indication of where you are in profitability while providing a cushion for better months. You should know the following at all times! How much are your operation expenses total monthly/annually (everything except payroll) to keep your business running you need this at a minimum? How much do you spend on payroll monthly? How profitable is each person on your team? How much does your marketing cost you? how long does it take to break even on that cost and ROI? What are your closing ratio numbers and how does that affect your business revenue? How much revenue have you grown in the last year compared to the previous? is this sustainable and how can you use this information to predict yr over yr growth? You should be able to make projections for the next year at a minimum. How to Calculate Payroll Costs For a Profitable Agency- https://www.youtube.com/watch?v=LOKUg9bVBes&t=142 Check Out The 15 Min Biz Quiz mentioned in the episode to learn what you need to fix next in your business! Let's BE FRIENDS UYB FB COMMUNITY: check out the awesome people in the tribe @femaleinsuranceagencyowners UYB INSTAGRAM: Come check out some awesome resources @unburden_your_business AGENCY BY DESIGN: If you're ready to take your business from stressed-out/maxed out to a money-making machine, come check out Agency By Design! https://www.unburdenyourbusiness.com/signup FACEBOOK GROUP: Come join our Facebook group. A safe space for Female Insurance Agency Owners to connect.https://www.facebook.com/groups/femaleinsuranceagencyowners Friend, make sure to say hi in the DMs I am all about getting to know more about you! So glad you're here, Cassidy

money roi challenge test
All Shows Feed | Horse Radio Network
Horse Tip Daily 1423 by Wintec Saddles: What is a Bute Challenge Test with Dr. Weber

All Shows Feed | Horse Radio Network

Play Episode Listen Later Dec 11, 2021 9:59


Ocala, FL based vet Dr. Elizabeth Weber explains  what a bute challenge test is. Support for this episode provided by Wintec SaddlesListen to more podcasts for horse people at Horse Radio NetworkThanks to today's tip contributor:  Dr. Weber, Miller & AssociatesLearn about advertising on Horse Tip Daily or any of the shows on the Horse Radio NetworkDownload the free Horse Radio Network App for iPhone or AndroidWe want your ideas on the tips you want to hear and if you have an idea on an expert to be on the show - click here to contact usSupport the show (https://www.patreon.com/user?u=87421)

iphone horses weber pony equestrian ocala saddles bute wintec challenge test horse radio network app
Horse Tip Daily
1423 by Wintec Saddles: What is a Bute Challenge Test with Dr. Weber

Horse Tip Daily

Play Episode Listen Later Dec 11, 2021 9:59


Ocala, FL based vet Dr. Elizabeth Weber explains  what a bute challenge test is. Support for this episode provided by Wintec SaddlesListen to more podcasts for horse people at Horse Radio NetworkThanks to today's tip contributor:  Dr. Weber, Miller & AssociatesLearn about advertising on Horse Tip Daily or any of the shows on the Horse Radio NetworkDownload the free Horse Radio Network App for iPhone or AndroidWe want your ideas on the tips you want to hear and if you have an idea on an expert to be on the show - click here to contact usSupport the show (https://www.patreon.com/user?u=87421)

iphone horses weber pony equestrian ocala saddles bute wintec challenge test horse radio network app
Scaling UP! H2O
208 The One About Great Communication, Generous Networking, and Authentic Relationships

Scaling UP! H2O

Play Episode Listen Later Aug 20, 2021 54:40


Scaling Up Nation, I cannot wait to introduce you to Paul Edwards. We met in our Mastermind group and have learned a great deal from one another. I've said it before, and I'll say it again, “When you meet new people, you open the door to new ideas and better ways of doing things that you never would have never thought of if you stayed alone in a silo.” Paul is a first-generation Spanish-speaking immigrant to the US with African heritage and Middle East combat experience. Paul's lived in five different countries, speaks two languages, and holds three passports. Paul wears many hats – he is CEO of a ghostwriting and content creation agency, bestselling author, podcast host, and one of the best people I know at networking. And if that wasn't enough, he is a husband, father, mentor, amateur theologian, men's physique athlete, voice mimic, and recovering insurance salesman.  Yes, Renaissance Man, Paul Edwards does a lot of things really well, but in today's episode, he is going to speak into the power of great communication, generous networking, and authentic relationships. When Paul isn't writing content for clients, you're most likely to find him building relationships and creating opportunities for his network. Bottom line: Paul does two things well: words and people. Your roadside friend, as you travel from client to client.   -Trace    Timestamps:  James' Challenge: Test for microbiological activity in your cooling systems. [5:22] Introducing author, speaker, and entrepreneur, Paul Edwards [8:25] Everyone has a book in them waiting to be written [11:50] Effective internal and external communication [24:10] The key to effective networking is generosity [29:10] The power of Masterminds, shared wisdom, and relationships [35:03] Lightning round questions [45:00]   Quotes: “Our lives are made richer by relationships.”  - Paul Edwards “Stories are compelling and relatable.”  - Paul Edwards “The core of what we do is that we make leaders into better communicators.”  - Paul Edwards “When you have leaders who are great communicators, great things happen.” - Paul Edwards “If you are communicating well, you should see more engagement or sales.” - Paul Edwards “When you are vulnerable, you disarm accusers and make others feel safe.”  - Paul Edwards “If you own a business, you need to run it like you could sell it anytime.” - Trace Blackmore “The book inside us is in the depths, not the shallow end.” - Paul Edwards “When you are writing a non-fiction book, the most boring thing you can do is tell them facts and figures.”  - Paul Edwards   Connect with Paul Edwards: Website: www.thepaulsedwards.com Email: pe@thepauledwards.com   Social: @ThePaulsEdwards   Paul Edward's Published Content: Influencer Networking Secrets - by Paul Edwards Business Beyond Business: How to Gain Magnetic Influence, Meaningful Connection and Profitable Publicity by Becoming a Radically Generous Entrepreneur - by Paul Edwards 10 Secrets to Networking Sucess: How to Build a Network of Super Affiliates That Endorse and Recommend Your Brand In Only 90 Days by - Paul Edwards   Links Mentioned: Episode 205 with Marty Stephens  The Rising Tide Mastermind Last Pass   Events: The US Water Treatment Conference November 3-6   AWT Webinar: Exiting Your Water Treatment Business: How to Prepare for an Eventual Transition – with Michael Warady October 29    Books Mentioned: Who Not How: The Formula to Achieve Bigger Goals Through Accelerating Teamwork - by Dan Sullivan The Common Path to Uncommon Success: A Roadmap to Financial Freedom and Fulfillment  - by John Lee Dumas  Business Secrets from the Bible: Spiritual Success Strategies for Financial Abundance - by Rabbi Daniel Lapin   

Scaling UP! H2O
198 Mark C. Winters

Scaling UP! H2O

Play Episode Listen Later Jun 11, 2021 65:38


On May 11, 2018, on Episode 38 Mark C. Winters the author of “Rocket Fuel ” was a guest on the show and he introduced the Scaling Up Nation to Entrepreneurial Operating System (EOS) in a blitz 100 level introductory course. Today I am pleased to have Mark C. Winters back on the podcast to walk us through his 200 level EOS course, which is all about communication.   If you are a new listener, or never had the chance to listen to Episode 38, please check it out today. It will immediately change your business, for the better.    In today's episode, Mark C. Winters teaches us how the Entrepreneurial Operating System (EOS) can specifically help businesses of 10-250 people climb a growth path to success, start their leadership teams with clear goals in mind, and confidently lay out which employee is going to do what to accomplish the company goals.    Additionally, Mark lays out the framework on how Visionaries (those who come up with the ideas) Integrators (those who make visions happen) can communicate better to take their companies to the next level. When Visionaries and Integrators have a great partnership, there is nothing they cannot do.  Bottom line: Mark C. Winters gave my company the tools, confidence, and energy we needed to accomplish more than I could have ever envisioned before we connected.    Your roadside friend, as you travel from client to client.     -Trace     Timestamps:   James' Challenge: “Test free chlorine in a cooling tower immediately and again an hour later from the same open sample.” [2:55]  Reintroducing EOS expert, and author of “Rocket Fuel ”, Mark C. Winters [6:45]  Best ways for a Visionary and an Integrator to work together, communication tools [13:54]  What do business owners need to know? [34:54]  How do you go about finding an Integrator for your business? [46:08]  Rocket Fuel University tools and networking [50:22]  What's the one thing you want everyone to know today? [55:00]  “I Said This, You Heard That “, Temperament communication strategies by Kathleen Edelman [56:08]  The 5 EOS Tools Mark uses to improve businesses [59:16]  Quotes:  “Simply said: The Visionary makes it up and The Integrator makes it happen.” - Mark C. Winters  “The worst thing we can do is to sit there stuck without moving forward.” - Mark C. Winters  “The Same Page Meeting Tool is a way to address issues. We want to be 100% aligned on the issues. Be a united front. You don't want to have conflict in the organization.” - Mark C. Winters   “The great duos understand each other and play to how they are wired.” - Mark C. Winters  “We're all different, the more I can understand that, the more effective we can be together.” - Mark C. Winters  “Understand each other better so we can do a better job getting to the truth and get aligned.” - Mark C. Winters  “The Accountability Chart: it's different from an org chart because it's not about levels and titles, it's about who is going to do it, and where the accountabilities lie.” - Mark C. Winters  “As a business owner, you have two rights: 1.) you have your right to the shares of the profit and 2.) your voice in the major decisions of the business. You don't have a right to be “an employee”, so if you are acting like an employee, you need to be the best solution to that issue or task.” - Mark C. Winters  “Understand who you are. And then search for your compliment on the other side of you that loves all the things you hate and stink at.” - Mark C. Winters  “The words we use are the gifts to the people who listen.” - Kathleen Edelman  “Learning about the Temperaments has been a game-changer on how I communicate” - Trace Blackmore  Mark C. Winters:  Mark C. Winters on LinkedIn  Rocket Fuel Video  Rocket Fuel the Book   Rocket Fuel Tools  Visionary/Integrator Assessments    Links Mentioned:  Episode 38 The One That Shows Us How to Run Our Business w/ Mark Winters  Submit a Show Idea  Episode 117 with Kathleen Edelman  Episode 179 with Kathleen Edelman  I Said This, You Heard That  by Kathleen Edelman  The Rising Tide Mastermind  Kolbe Index   Culture Index  DiSC Assessment     Events:  The Hang Networking Event- @6pm on June 10th    Books Mentioned:  Rocket Fuel the Book - by Mark C. Winters   Traction - by Gino Wickman  Who Not How: The Formula to Achieve Bigger Goals Through Accelerating Teamwork - by Dan Sullivan   I Said This, You Heard That Study Guide - by Kathleen Edelman   Strength Finder 2.0 - Tom Rath and Gallup   What the Heck is EOS- by Gino Wickman and Tom Bouwer   

TheRightDoctors
6 MWD 6 minute walk challenge test

TheRightDoctors

Play Episode Listen Later Jul 2, 2020 4:49


Cardio-Diabetes Specialist

minute walk challenge test
Our Community Listens Podcast
Mini Refresher - Parents with Kids Ages 0-3

Our Community Listens Podcast

Play Episode Listen Later May 2, 2020 6:55


Do you have a child between the ages of 0-3 in your home? Listen in to hear about some normal reactions you and your partner may be having right now and how you can be connecting through these changes in your family. CHALLENGE: Test out your favorite OCL skill with your child. What went well? What might you change next time? PODCAST: https://soundcloud.com/ourcommunitylistens/epi-041 BLOG: https://www.ourcommunitylistens.org/blog/post/michigan/2018/09/20/what-is-empathy-and-can-i-get-better-at-it https://www.ourcommunitylistens.org/

A Phil Svitek Podcast - A Series From Your 360 Creative Coach
21-Day No-Complaint Challenge! Test It Out for Yourself!!!

A Phil Svitek Podcast - A Series From Your 360 Creative Coach

Play Episode Listen Later Apr 19, 2020 3:48


Tim Ferriss (@TimFerriss) introduced me to the 21-day no-complaints challenge and I am going to take it. And I encourage you to try it as well. Complaints are a form of negativity that drag you down rather than fix anything. In this episode I go over my thoughts heading into the challenge, since I don't think it'll be a piece of cake. I overview how little thoughts we have add up to a lot. But I'm up for the challenge and I hope you are too! Let's keep hope alive and spread goodness with this. Comment down below with any questions or thoughts that you may have, or most importantly—your experience doing this challenge. Hit me up on social media @PhilSvitek if it's easier. I'd love to hear from you. And for more free resources from your 360 creative coach, check out my website at http://philsvitek.com.

Solo Biz Bites
S2 - Email Marketing Challenge - Test & Tweak

Solo Biz Bites

Play Episode Listen Later Sep 24, 2019 6:51


What is the difference between a rock star email marketer and everyone else? It is not a huge staff or abundant resources (those help) - it is the ability to test and tweak to improve their marketing for their audience. In this episode, we tackle test and tweaking to help you serve your audience better and make more sales.

email marketing tweak challenge test
Encouragementology
Patience, Everything in Time

Encouragementology

Play Episode Listen Later Jun 25, 2019 35:51


So they say patience is a virtue but what does that mean? It is defined as behavior showing high moral standards is the definition but why is that important to our day-to-day. Of course, having high moral standards is important but it doesn’t tell us the value of patience.  Every great dream begins with a dreamer. Always remember, you have within you the strength, the patience, and the passion to reach for the stars to change the world. Harriet Tubman Patience is not simply the ability to wait - it's how we behave while we're waiting. Joyce Meyer Each life is made up of mistakes and learning, waiting and growing, practicing patience and being persistent. Billy Graham Lots of good advice there but it doesn’t make the process any easier. Patience and be painful and sometimes what trumps success. Let’s look at it from a few didn’t perspectives to see if I can shine some new light on some strategies.  Patience requires restraint. In a world that moves by at the speed of light without much thought to the overall picture or quality of life...patience requires us to slow down and be mindful.   When we feel like we have all the answers and with a little more hard work we can make it happen and happen faster, patience makes us wait. Relax and wait. Where timing and growth meet.  When we are frustrated because nothing is turning out the way we expected and we just can’t seem to catch a break, patience reminds us that life is still moving and things are still evolving and we aren’t finished yet.  Another perspective says: "Patience: the capacity to accept or tolerate delay, difficulty, or annoyance without getting angry or upset."  Notice how impatience arises when we’re not getting our way—specifically, when people or our environment aren’t conforming to our expectations, even in circumstances over that we have no control (for example, the flow of traffic or the length of a line). Our expectations are often out of synch with reality.  Recognize the stressful feeling, and FREEZE FRAME it. Take a time out. Make a sincere effort to shift your focus away from the racing mind or disturbed emotions to the area around your heart. You can pretend that you are breathing thru your heart to help focus energy in this area. Keep your focus there for ten seconds or more. Recall a positive, fun feeling or time you’ve had in life and attempt to re-experience it. Now use your intuition, common sense and sincerity-- ask your heart, what would be a more efficient response to the situation, one that will minimize future stress? Listen to what your heart says in answer to your question. It's an effective way to put your reactive mind and emotions in check and an in-house source of common sense solutions. These are just ideas and strategies to try and isn’t that just it…..not sure anyone is born with patience - you lose your cool, your force things to happen, you fall on your face, things don’t work out the way you want, then hopefully….you regroup and try something new. …..is that where you are? By cultivating a practice of patience, you’re able to let go of things outside your control and live with less stress, anxiety, and frustration. When you are patient you are still and when you are still you have a chance to listen, and when you listen the universe speaks. Challenge: Test your patience and wait - wait before anger, wait before sadness, wait before giving up. Practice patience as you listen to and for direction. Show patience with those you meet - listen, understand, encourage. There is power in patience.  I know YOU can do it!

Greatness Quest
#156: MORNING ROUTINE LIFE-HACK - Daily Mentoring w/ Trevor Crane #greatnessquest

Greatness Quest

Play Episode Listen Later Sep 4, 2018 15:09


MORNING ROUTINE LIFE-HACK #156: Daily Mentoring with Trevor Crane on GreatnessQuest.com SUMMARY Here’s a little morning routine, and LIKE HACK, I think you’re going to love. I’ve been testing it out now for over two weeks and I love it. It’s improved my happiness my productivity my profitability and I’m finding new insights inside myself that have been profound... CHALLENGE: Test this out for yourself and let me know how it works for you! GET THE APP: Text: TREVOR To: 36260 #greatnessquest #trevorcrane #unstoppable #idealbusiness #ideallife

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First Baptist Church Temple

Matthew 13:3-9 Then he told them many things in parables saying, “A farmer went out to sow his seed. As he was scattering the seed, some fell along the path, and the birds came and ate it up. Some fell on rocky places, where it did not have much soil. It sprang up quickly, because the soil was shallow. But then the sun came up, the plants were scorched, and they withered because they had no root. Other seed fell among thorns, which grew up and choked the plants. Still other seed fell on good soil, where it produced a crop – a hundred, sixty or thirty times what was sown. Whoever has ears, let them hear.” Some seed gets snatched.What distractions are snatching the seed from your life? Some seed gets scorched.In what is your life rooted?Some seed gets strangled.Change your mind and change your life.Matthew 13: 24-28 Jesus told them another parable: “The kingdom of heaven is like a man who sowed good seed in his field. But while everyone was sleeping, his enemy came and sowed weeds among the wheat, and went away. When the wheat sprouted and formed heads, then the weeds also appeared. The owner's servants came to him and said, ‘Sir, didn't you sow good seed in your field? Where then did the weeds come from? An enemy did this,' he replied. Some seed gets sabotaged.The enemy can't take your destiny but he can cause you not to recognize it.Challenge: Test your soil.

First Baptist Church Temple

I. Spiritual activity is not necessarily Godly activity. (There is a spiritual world.) A. Stop believing every spirit! B. Test the spirits! C. What should one test for? 1. Test prophets by what they think about Jesus. 2. The test is not only comprehensive, it is confessional.II. Test for consistency! (1 John 4:4-6) A. God's people know his voice. B. God's people have overcome the false prophets. C. God's people are possessed by the Holy Spirit. D. Don't be surprised by false prophets. E. Trust your hearing.Challenge: Test whatever you hear about Jesus against the Bible.

Medizin - Open Access LMU - Teil 17/22
Release of Mast Cell Tryptase into Saliva: A Tool to Diagnose Food Allergy by a Mucosal Challenge Test?

Medizin - Open Access LMU - Teil 17/22

Play Episode Listen Later Jan 1, 2011


Background: Our aim was to examine whether measurement of the saliva mast cell tryptase (MCT) concentrations before and after a mucosal challenge test with the offending food would be helpful in diagnosing food allergy. Methods: We performed a retrospective analysis of 44 food challenge tests performed in 38 patients between 2006 and 2009. Patients with a suspected history of food allergy chewed the food until they developed symptoms or until the amount of time known from the patients' history to usually be required for the provocation of symptoms had passed. In 5 patients, saliva samples for the measurement of MCT were collected at minutes 0, 1, 4, 8, 11, and 16 after the first onset of symptoms. The remainder of the patients only had samples taken before chewing and 4 min after the end of the test period. Results: During repeated measurements, MCT peaked about 4 min after the onset of symptoms (p = 0.028). During 33 of the 44 tests (75.0%), we observed oral symptoms during testing; after 25 of the 33 (75.8%) tests evoking symptoms, the saliva MCT concentration increased. The MCT increase was negative in all other tests where no oral symptoms could be provoked. Conclusions: The measurement of saliva MCT 4 min after the onset of symptoms may be helpful to diagnose food allergy. Because of numerous confounding variables, however, a negative saliva MCT increase does not exclude food allergy. Copyright (C) 2011 S. Karger AG, Basel

patients tool diagnose medizin basel food allergies saliva mct mast cell mucosal copyright c challenge test results during karger ag
Tierärztliche Fakultät - Digitale Hochschulschriften der LMU - Teil 04/07
Untersuchungen zum Vorkommen und zur Toxizität von Bacillus cereus in Gewürzen

Tierärztliche Fakultät - Digitale Hochschulschriften der LMU - Teil 04/07

Play Episode Listen Later Jul 18, 2008


B. cereus zählt zu den wichtigsten Verursachern von Qualitätsminderung und Verderb bei Lebensmitteln. Daneben wächst die Bedeutung Toxin-bildender B. cereus Stämme als Auslöser Lebensmittel-bedingter Erkrankungen, die zwei Formen einer gastrointestentinalen Erkrankung hervorrufen können: das diarrhoeische Syndrom, welches durch verschiedene Enterotoxinkomplexe (HBL, Nhe) induziert wird, und das emetische Syndrom, welches durch ein Dodekadepsipetid (Cereulid) ausgelöst wird. In komplexen Lebensmitteln werden vielfach Gewürze als Vektor für B. cereus - Kontaminationen angesehen. Jedoch sind kaum Studien über Gewürze als mögliche Eintragsquelle für B. cereus in Lebensmittel publiziert. Auch liegen nur wenig aktuelle Daten aus dem europäischen Raum über die tatsächliche Belastung von Gewürzen mit diesem Erreger vor. Ziel dieser Arbeit war es, das Vorkommen und die Toxizität von B. cereus in Gewürzen analysieren, um eine aktuelle Übersicht über die Kontamination mit diesem Erreger für eine Bewertung der mikrobiologischen Sicherheit von Gewürzen zu gewinnen. Hierfür wurden insgesamt 60 Gewürzproben zwölf verschiedener Gewürzsorten untersucht. Zunächst wurde mittels kultureller Verfahren die aerobe mesophile Gesamtkeimzahl sowie die Belastung mit Enterobacteriaceae und präsumtiven B. cereus bestimmt. Um auch kleinste Mengen des Erregers sicher nachweisen zu können, wurde zudem ein Real-Time PCR Assay als Alternative zur zeitaufwendigen und diagnostisch ungenauen konventionellen MPN- Methode erarbeitet. Im weiteren Verlauf der Arbeit wurden 151 präsumtive Kolonien, die aus den untersuchten Gewürzproben (n=60) isoliert wurden, mittels biochemischer und molekularbiologischer Analyse als B. cereus bestätigt. Anschließend wurde die Toxigenität und Toxizität der Isolate mittels PCR, immunchemischer Nachweisverfahren (ELISA, RPLA) sowie im Zellkulturtest charakterisiert. Darüberhinaus wurde im Challenge-Test mit marinierten Fleischerzeugnissen untersucht, inwieweit eine Kontamination von Lebensmitteln mit B. cereus tatsächlich aus der Verwendung kontaminierter Gewürze resultiert. Nach mikrobiologischer Analyse der Gewürzprodukte (n=60), waren nahezu alle der Proben als mikrobiologisch unbedenklich einzustufen. Kein Produkt hat den von der DGHM und der Europäischen Kommission empfohlenen Richtwert für B. cereus in Gewürzen von 103 KbE/g überschritten. Mit dem Real-Time PCR-Assay wurde zudem eine zuverlässige Alternative zum konventionellen MPN - Verfahren erarbeitet, der es ermöglichte auch kleinste B. cereus Mengen nach selektiver Anreicherung in den Gewürzproben zu identifizieren. Dies ist insbesondere im Hinblick auf das gesundheitsgefährdende Potential pathogener B. cereus von Bedeutung. So wurden 81% der Isolate (n=151), die aus den Gewürzen gewonnen wurden als diarrhöische und/oder emetische Toxinbildner identifiziert. Zudem wurde gezeigt, dass eine B. cereus Kontamination von Lebensmitteln durchaus aus der Verwendung damit hergestellter, belasteter Gewürze resultieren kann. Jedoch ist das Risiko für den Verbraucher hinsichtlich einer Lebensmittelvergiftung durch kontaminierte Gewürze als gering einzustufen. Zwar wurde B. cereus in 70 % der insgesamt 60 untersuchten Proben detektiert, allerdings in Keimzahlen, die nach derzeitigem Stand der Wissenschaft keine Lebensmittelvergiftung auslösen können. Dennoch ist das Gesundheitsrisiko, das von toxinbildenden B. cereus ausgeht nicht zu unterschätzen, da sie als Sporenbildner Erhitzungsprozesse von Lebensmitteln überleben, wieder auskeimen und sich vermehren können. Das Vorkommen von B. cereus in Gewürzen ist somit unter den Gesichtspunkten eines vorbeugenden Verbraucherschutzes und der Qualitätssicherung zu betrachten, nicht zuletzt da es auch angesichts eines stetig wachsenden Welthandels im Zuge der Globalisierung immer dringlicher wird eine mikrobiologisch unbedenkliche Ware zu gewährleisten. Letztendlich sind weitere Untersuchungen mit größeren Probenzahlen für eine besserere Risikoabschätzung unumgänglich. Mit dem Real-Time PCR Assay steht hierfür eine schnelle und zuverlässige Methode zur Verfügung, die einen Erregernachweis direkt aus Gewürzproben erlaubt.