Podcasts about bhk

In this episode Pierre-Marie Pédrot, one of the main Coq/Rocq developers joins us to talk about Krivine, Kleene and Gödel Realizability Models, how it relates to the BHK interpretation and CPS Translations, and how it was all already part of Gödel's work in Dialectica! If you enjoy the show please consider supporting us at our ko-fi: https://ko-fi.com/typetheoryforall Links Pierre-Marie's Website Pierre-Marie's PhD Thesis (Very nice read) BHK Interpretation Type Theory Forall website Type Theory Forall discord

In today's episode, I talk with osteopath, Brendon Talbot, BHK, M.OMSc about his educational background, osteopathic training and how he treats individuals with musculoskeletal disorders.  Here is a bit more about Brendon from his website:  "Motivated by the body's innate ability to self heal and self regulate, Brendon prides himself on providing the highest quality of Osteopathic care. Brendon always works with his best efforts, to ensure the patient receives the best possible treatment each session, while working safely within the scope of Osteopathy. Understanding human anatomy and physiology in a collective manner helps Brendon provide a personalized treatment for every patient.  Prior to his osteopathic journey, Brendon's appreciation of collective mechanics stems from his many years as an elite athlete. He expresses undeniable passion and interest for his understanding of the human body. Treatment outcome aims to reduce symptom expression, improve overall quality of life, speed the restoration of normal function and reduce the impact of illness on home and work life. “I love to educate patients and encourage a healthy lifestyle to further optimize and improve the patients quality of life.” You can learn more about Brendon on his website or his Instagram account (@bt.osteopathy). 

On this segment of Copper Country Today, as BHK Child Development celebrates 50 years of service to the Copper Country, host Todd VanDyke talks with Katie Waara, Katie Hardy and Cheryl Mills about Head Start and the other programs BHK offers. Copper Country Today airs throughout Michigan's Keweenaw Peninsula Sunday mornings at 7:00 on WOLV 97.7 FM, 8:00 on WCCY 99.3 FM and 1400 AM, and 9:00 on WHKB 102.3 FM. The program is sponsored by the Copper Shores Community Health Foundation. Copyright © 2024, Houghton Community Broadcasting, Houghton, MI.

Bogus loses his voice. Brut loses his track of time (and his senses). Both hope and dream that one day, they can each afford a 3 BHK penthouse at the prestigious Aghora Heights.____________________Subscribe, and Share!***Patreon: patreon.com/ThyGapInstagram: @_ThyGap |Twitter: @ThyGap |Email: mindthygap@gmail.com |Discord: https://discord.gg/mPS4aNWa94 |All Links: https://linktr.ee/thygap |

Bogus loses his voice. Brut loses his track of time (and his senses). Both hope and dream that one day, they can each afford a 3 BHK penthouse at the prestigious Aghora Heights.____________________Subscribe, and Share!***Instagram: @_ThyGap |Twitter: @ThyGap |Email: mindthygap@gmail.com |Discord: https://discord.gg/mPS4aNWa94All Links: https://linktr.ee/thygap

罄竹難書的罪惡曾經暢行無阻， 正義也曾躲在窗後暗自哭泣。 當良知逐漸覺醒， 黑魔王還有多久會走上自己打造的死路？ 善，畢竟存在， 屠夫的倒台是天命使然， 或時間的自然修正？ 【BHK's 紅萃蔓越莓益生菌】

Dans cet épisode de RAP NEWS, je vous présente les dernières actualités du monde du rap. Au programme, je mets en avant le festival Impulsion à Aubagne, je vous parle de la rappeuse EKLOZ qui a lancé une cagnotte avec des contreparties alléchantes, ainsi que de la sortie de mon dernier clip "Pas Révisé", un freestyle de rap de 3 minutes qui a reçu des retours élogieux. Je vous informe également sur les sorties singles de la semaine, incluant des artistes tels que SHINZO, BHK, ZAMDANE, REMY (qui fait son grand retour) et la découverte de la semaine GEKA. Enfin, j'ai sélectionné trois albums à écouter absolument: SKIA, ASHEO et HICHAM, qui ont tous reçu des critiques positives. Ne manquez pas cet épisode de RAP NEWS pour être à jour sur toutes les dernières nouvelles de la scène rap. La cagnotte d'EKLOZ: https://fr.ulule.com/ekloz-aide-moi-a-financer-mon-prochain-clip/?utm_campaign=presale_161472&utm_source=shared-from-Ulule-project-page-on---http.referer-- --- Send in a voice message: https://podcasters.spotify.com/pod/show/tyler-le-media/message

In this episode of Why Not Mint Money, Mint's Maulik speaks with Mayank Dhingra, an automotive journalist on his home-buying experience. Mayank and his wife, Neha Lalwani bought their first-ever flat, a 1 BHK in Shalimar Bagh in West Delhi in November 2021. We speak with Mayank on how the two went about shortlisting the right flat for themselves and what factors influenced their decision. Did they face any challenges along the way? Let's hear it from him.

JAH BLESS AND WELCOME TO "KINGS COURT BINARY BASHMENT" WITH PODCAST SELECTOR KINGRASTA28 DA ELDA: Source Chi:king Michael Jackson:i'm a muslim Seenic The BlaqStarr:state of emergency Aisha Sekhment::my enemies try to bring me down Ice Cube ft Chuck D:endangered species tales from the darkside Foundation&Rezidue:dont get it twisted Run-Dmc ft Ice Cube&Chuck D:back from hell(remix) Medusa ft Mc Lyte:lay down Erbsun:everlasting LadyStunn:fatdaddyk Lunch&Nique:i'm whoa Foundation&Bill Biggs:nameless              Dj Premir ft Remy Ma&Rapsody:remy rap  Lady Of Rage ft Diamonique:put u up on rage J-Dee ft Kokane:j-deed's revenage Papoose ft Remy Ma:Broken Slanguage Bad Azz&The Lady Of Rage/Legacy:a hold on hip hop Foxy Brown ft Mia X&Gangsta Boo:b.w.a GangStarr ft Lady Of Rage&Kurupt:you know my steez Lil Marvaless ft Marvaless:like mother like daughter Joel Ortiz ft Cashmere/Manio/Big Daddy Kane:brooklyn(remix) Foundation&Rezidue:boogie down got the flavor DJ Quick ft Mc Eiht&Problem:central ave Will I Am ft Medusa:hooda hella u Ms Jade ft Big Daddy Kane&Biggie:set it off Royzy Rothschild ft Emily Callacher: lonely road Rick James ft Roxanne Shante:loosey's rap Mimic:crackin whats happenin AL-Fila:keep it crackin          

Learning what our mind, body, and soul truly needs takes time, life experience, and an openness to let go and connect with what serves our highest good. I am so excited to have Founder of Vancouver Stretch Therapy, Asma Kassam, BHK, CSNA, CFST, join our tea time and share her story. Asma has been in the health and fitness industry for more than half of her life and has worn many hats throughout her journey. Listen in as we talk about re-patterning narratives that no longer serve us, as well as having those lightbulb "aha" moments with our mind, body, and soul. We dive into the importance of breath work, connecting with your feminine body, and finding the flow of your true alignment. Asma drops so much amazing wisdom on us, so make some room in your toolbox of life because we've got more for you to add in there! Cheers and let's spill!

On Cock & Bull today, we are joined by Anupam, Ayushi & Niveditha. Today Cyrus is expressing the need to have a 2 BHK flat as Mumbai now is nothing but a city of construction. In the show: Anupam - host of the Paisa Vaisa podcast, introduces us to his brand new book called ‘The Wisest Owl' which is now available to buy, Cyrus exposes Anupam's riches everywhere around Mumbai city, Ayushi & Niveditha both are super late for the show, and Anupam answers many... Many questions related to the finance world. And, of course, a lot of unmatched chaos. So Tune in for this and much more!Check out Cyrus Says merch:ivm.today/3PLKo1mYou can follow Anupam on Instagram at @b_50You can follow Ayushi on Instagram at @ayushia9You can follow Niveditha on Instagram at @niv.prakasamStream the podcast on major platforms like Spotify, Apple Podcasts, & Google PodcastsDo send in AMA questions for Cyrus by tweeting them to @cyrussaysin or emailing them at whatcyrussays@gmail.comDon't forget to follow Cyrus Broacha on Instagram @cyrus_broacha (https://www.instagram.com/cyrus_broacha)In case you're late to the party and want to catch up on previous episodes of Cyrus Says you can do so at: www.ivmpodcasts.com/cyrussaysYou can listen to this show and other awesome shows on the new and improved IVM Podcasts App on Android: https://ivm.today/android or iOS:https://ivm.today/ios

In this episode, Darren and Duncan reflect on their experiences with friend and mentor Bascom King, whose recent passing has taken a great mind from the HiFi community. Bascom was an audiophile engineering legend and responsible for many incredible amplification designs over a long career. Hear about how he inspired so many, and learn a little about his upcoming posthumous amplifier release, the BHK 600 amplifiers. The guys also read a couple of emails, and this week's album recommendation comes by way of Australia and is one to not miss.

Guest: Linden Birns | partner at BHK crisis communications See omnystudio.com/listener for privacy information.

Sam Reynolds started his career as Thomas Swan as an Electrical Maintenance Engineer, before moving to an automotive ball bearing manufacturer for 5 years, where he first got into management at an early age  For the last 3 and half years Sam has worked for Peterlee based BHK,  a market leader for  manufacturing drawers in the UK.  and is currently their Engineering Manager  He's also been successful with his Music career and toured with some of huge household names.  In this podcast we delve into what it's like to manage at such an early age, how he simplifies management by understand the fact that people are humans and you just need to communicate effectively. There are some great tips here for younger manages perhaps lacking confidence and wanting to progress in their careers.Please subscribe to the channel for more content! Theo James are a Manufacturing & Engineering Recruiter based in the North East, helping Manufacturing and Engineering firms grow across the UK. Please call us on 0191 5111 298

In this episode, Sara Hodson of Live Well Exercise Clinic and President of the Fitness Industry for Canada, shares not only about physical fitness, but she also shares her wisdom on mental and social health. Sara geeks out a bit on the science of exercise and an analogy of the covid forest fire we've experienced in the economy and business.    Highlights From This Episode: The top 2 barriers to starting any type of fitness lifestyle Prioritizing your health is no different from your business strategy, plans, goals and weekly action plans Having your fitness time in your calendar really sets you free from the guilt Mental health and a state of languishing Our social health is really paramount right now   Links & Resources From This Episode: Learn more:   Live Well Clinic   More About SARA HODSON, BHK, CSEP-CEP, ACSM-CCEP PRESIDENT, Fitness Industry Canada (FIC) FOUNDER & CEO, LIVE WELL Exercise Clinic  ACSM CERTIFIED Clinical Exercise Physiologist Sara Hodson has spent her 20-year career devoted to how exercise affects our physical and mental health. A trained Clinical Exercise Physiologist, Hodson began her career in cardiac rehabilitation and diabetes management for Fraser Health Authority in BC. There, she saw a need for patients to have clinically supervised medical fitness programs beyond the hospital walls. In 2011 she launched LIVE WELL Exercise Clinic – a safe, supportive, supervised exercise environment focused on the management and prevention of chronic health conditions. Her clinics create an inclusive culture where everyone is supported in their health journey. In the last ten years, LIVE WELL has grown as a community with franchises in four Canadian provinces, and since COVID-19 began includes a virtual platform to serve members all over the world. Sara lives and breathes exercise as medicine and is a sought-after speaker on the dose effect of exercise for physical and mental health. Sara is also the proud mother of four teenagers. Awesome FREE Resources for YOU! Power of 3 Daily Planner Check out our other podcast guests!   Thanks for listening! Thanks so much for listening to our podcast! If you enjoyed this episode and think that others could benefit from listening, please share it using the social media buttons on this page. Do you have some feedback or questions about this episode? Leave a note in the comment section below!   Subscribe to the podcast If you would like to get automatic updates of new podcast episodes, you can subscribe to the podcast on iTunes or Stitcher. You can also subscribe from the podcast app on your mobile device.   Leave us an iTunes review Ratings and reviews from our listeners are extremely valuable to us and greatly appreciated. They expose our show to more awesome listeners like you. If you have a minute, please leave a review on iTunes. Val Low is a digital & efficiency expert to entrepreneurs to clear the business clutter and thrive. Through her Focus&Freedom™ Method many entrepreneurs have experienced more focus, time and freedom. Val shows entrepreneurs how to calm the overwhelm and distractions with focus and efficiency strategies and tools that enable them to have the freedom to do the things that matter most in their business and life. Through her Magnify Your Brand™ program, entrepreneurs and experts clarify their message that attracts their ideal clients, a brand experience that people love to talk about and setting up in a one place online platform that makes it easier for entrepreneurs to spend time in their expertise. Visit Val's Website: https://vallow.me/ Connect with Val on Facebook: https://www.facebook.com/ValLow.page/ Connect with Val on Linked In: https://www.linkedin.com/in/val-low #sales #entrepreneur  #business  #branding  #marketing #mindset  #success  #leadership  #focus  #coaching  #fitness   #health  #Live Well  #health

This week in Indian Startup News, Ola to set up the world's largest two-wheeler EV charging network, Tvasta builds India's first 3D printed house, Zomato files for IPO, Indian startups fight against COVID19, CCI approves BigBasket-Tata deal, Lenskart acquihires DailyJoy and Instamojo acquihires Showman. In funding news, OneWeb raises $550 million, Urban Company raises $190 million, upGrad raises $120 million, Stanza Living raises over $100 million, ElasticRun raises $75 million, Bizongo raises $51 million and LEAD School raises $30 million. Ola to set up world's largest two-wheeler EV charging network: Ola is setting up the world's largest two-wheeler EV charging network – Hypercharger Network. Their Hypercharger Network will consist of 100,000 charging points across 400 cities – expected to be completed in the next five years at an estimated cost of $2 billion. You will only be able to charge Ola's electric scooters at their hypercharger network and they will be able to charge the electric scooters up to 50% in just 18minutes – giving a range of 75 km. Tvasta builds India's first 3D printed house: Tvasta, a startup by three IIT Madras alumni, has built India's first 3D printed house. This is a 1 BHK house with a built-up area of 600 square feet. It demonstrates the ability of 3D printing in bringing down the time of building houses from 4-5 months to just 5 days but also reducing the cost by 20-30%. Zomato files for IPO: Indian food delivery startup Zomato has filed for the IPO – on track to becoming the first Indian unicorn to go for IPO. Through the IPO, Zomato will be raising $1.1 billion - $1 billion in fresh offering and $100 million coming from Info Edge selling their shares. Indian startups fight against COVID19: A bunch of Indian startups are working to fight the second wave of COVID19 – Delhivery is flying chargers to bring Oxygen concentrators into India, dating app TrulyMadly is using their matchmaking algorithm to match plasma donors with patients, NoBroker is organizing doorstep vaccination drives and MapmyIndia is installing free GPS devices in vehicles that are suppling essential medical equipment. CCI approves BigBasket-Tata deal: CCI has approved the acquisition of BigBasket by Tata Digital – as Tata looks to build their own Super App. Lenskart acquihires DailyJoy: Lenskart has acquihired Hyderabad-based grocery delivery startup DailyJoy to strengthen their Hyderabad tech centre. Instamojo acquihires Showman: Instamojo has acquihired vernacular content startup Showman to strengthen its tech and product capabilities. OneWeb raises $550 million: Satellite internet company OneWeb has raised $550 million from Eutelsat Communications after launching 36 more satellites into space. The company now has 182 out of 648 satellites in space for their constellation. Urban Company raises $190 million: On-demand home services provider Urban Company has raised $190 million at a $2 billion valuation – making it India's 11th unicorn this year. upGrad raises $120 million: Edtech startup upGrad has raised $120 million from Singapore's Temasek in their first external funding round. Stanza Living raises over $100 million: Student housing startup Stanza Living has raised more than $100 million in a round led by Falcon Edge. ElasticRun raises $75 million: ElstaicRun has raised $75 million in a round led by Avataar Ventures and Prosus Ventures to help neighbourhood stores in securing working capital and inventory. Bizongo raises $51 million: B2B platform for packaging products Bizongo has raised $51 million in their Series C round to expand into the Southeast Asian markets. LEAD School raises $30 million: Edtech startup LEAD School has raised $30 million in a round led by GSV Ventures and WestBridge Capital.

HTT.01 欢迎来到BHK !! : 如何在英国上家里蹲大学？ GG Channel 我们不止聊动漫！ 一档讨论ACG及其相关内容的动漫播客，欢迎加入催更群：553943741 主播：德国咕科 嘉宾：三替 节目简介：这期HTT是新开的一个下午茶闲聊系列节目，世界各地的留学生们学习生活中各种文化冲击或者旅游话题。我们交谈各种生活琐碎，顺便散发一些负面情绪（划掉）。 这期主要是交流一些因为疫情导致的不得不在国外当家里蹲的无聊又有一点特别的生活。

Spotify 被員工綁架，先是刪掉右派內容，現在員工更以冒犯 LGBTQIA+ 族群為由，要求全面審查旗下創作者的內容否則甚至要罷工；一家公司有競爭外患不打緊，內憂耍憨才是最可怕的，現在看起來 Spotify 若不振作，很快就會被夾殺出場。談一下我覺得 TikTok 事件的荒謬之處，若你覺得真的有國安危機，就該直接循印度模式 ban 掉；但看起來大家都想要分一杯羹，最後還是回到$$利益問題，而國家安全只是師出有名的大旗而已，行為裡變成次要目標。 本集節目由BHK's無瑕机力贊助 BHK's 無瑕机力是台灣保健食品品牌有一系列針對全家大小、孕婦、兒童的保健食品。BHK's 金盞花葉黃素很適合當作每日營養品，3 盒只要 690 元。 加入BHK's官網會員現折30元，生日當月還會有 100 元生日禮金。 https://bhks.cc/Q055P1.html --- 家樂福線上購物想邀請菜雞上車一起大口吃肉。9/30 日前註冊線上會員送 300，輸入折扣碼 GOOAYE 1800 再折 200，首購只要買滿 499 就免運。 https://bit.ly/3hBpf7r

本集節目由《BHK's無瑕机力》贊助撥出。 10個女性有9個有私密問題！ 女生們就是要好好愛護自己，做好私密的保養。 BHK's紅萃蔓越莓益生菌錠，這裡選購→ https://bhks.cc/Q006P1.html 《生活市集》現在99購物節來生活市集，限時破盤商品狂降，買完記得到敏迪專屬活動連結登記，消費不限金額前99名不限金額登記直接送(不用抽)肯德基蛋塔 超好吃の舒肥雞胸： https://ms7.tw/6vvlo 前99名登記送肯德基蛋塔：https://ms7.tw/qH4aw 《SBD Taiwan》SBD為菁英運動員製造出世界上最好的配備，讓每位運動員突破自己，SBD為你撐腰。現在只要上SBD官網，購買任一商品輸入折扣碼Mindy，就可享有敏迪選讀專屬優惠喔！https://reurl.cc/A8KVoY 本週新聞重點 00:04:23 疫情整理 00:15:43 WTO秘書長換誰當家？ 00:29:41 塞爾維亞和科索沃，到底吵什麼？（上） 00:49:42 塞爾維亞和科索沃，到底簽了什麼協議（下） 01:06:41 什麼？！川普獲提名諾貝爾和平獎！？ 01:16:04 結尾：阿爆演唱會心得，9/26高雄場還有票喔！ 敏迪持續在找podcast廣告乾爹，歡迎來信mindyworldnews@gmail.com 歡迎大家下載敏迪選讀專屬App iOS：https://apple.co/2tgFKCu Android：http://bit.ly/2RXh6RS 如果你喜歡我每天早晨寫給你的文字， 歡迎用每個月59元支持我 敏迪選讀訂閱（嘖嘖） https://www.zeczec.com/projects/mindynews Instragram：https://www.instagram.com/mindiworldnews/ Telegram：https://t.me/mindiworldnews

本集節目由BHK's無瑕机力贊助 台灣每10人就有1人睡不好！ 補充BHK's夜萃EX，可以幫助入睡、調節入睡品質，隔天才能有好氣色。 至官網選購→ https://bhks.cc/Q022P1.html 百萬感謝季直播由此去： https://www.youtube.com/watch?v=iNX61iQdcm8 本集綱要： 「節食最容易遇到的兩個心理關卡，分別是好棒棒效應跟我就爛效應」 本集重點 。意志力是什麼 。做了點好事天使就說你好棒棒 。多吸一點不健康食物的味道，會讓你更願意選擇健康飲食 。破戒了，好吧我就爛 覺得節目不錯嗎？歡迎來跟我們聊聊 哇賽！心理學 IG帳號 www.instagram.com/onyourpsy/ Telegram頻道 t.me/onyourpsy ___ 主談：蔡宇哲 Powered by Firstory Hosting

For our Hebrew Bibles we have three main groups of manuscripts to compare: the Masoretic Text group, the Samaritan Pentateuch group, and the Septuagint group.  Each of these groups contain many manuscripts with the Masoretic Text group containing the most.  In this episode of How We Got the Bible, we’re going to look at the Read more about 333 Bible 4 – Determining the Best Hebrew Reading[…]

For our Hebrew Bibles we have three main groups of manuscripts to compare: the Masoretic Text group, the Samaritan Pentateuch group, and the Septuagint group.  Each of these groups contain many manuscripts with the Masoretic Text group containing the most.  In this episode of How We Got the Bible, we’re going to look at the Read more about 333 Bible 4 – Determining the Best Hebrew Reading[…]

Dr. Joseph Peter Barsuglia is a clinical psychologist focused on bridging ancestral medicines and wisdom with best clinical practices, in order to catalyze personal healing and spiritual awakening. He has worked as Research Director of a psychedelic treatment center which utilized 5-MeO-DMT and ibogaine for the treatment of addiction, as a psychotherapist in the MAPS-sponsored trials of MDMA-assisted psychotherapy for PTSD, and is an initiate of the African tradition called Bwiti which uses iboga as its sacrament. In this episode, Dr. Barsuglia discusses what happens to the body when 5-MEO-DMT is consumed. He also discusses the origins and history of 5-MEO-DMT, as well as some of the scientific research being conducted on the molecule. He also shares some research on how he observed the mystical effects 5-MEO-DMT had on patients and some of the consistent experiences patients described.  The increasing popularity of this psychedelic often brings up a discussion on the environmental impact of harvesting Sonoran Desert toads. Dr. Barsuglia uses this to discuss differences between synthetic vs naturally occurring 5-MEO-DMT, and how synthetic developments can help protect the environment and preserve tribal tradition.    In this episode: The sources of 5-MEO-DMT and how it is used The historical, cultural, and environmental significance of 5-MEO-DMT The effects of 5-MEO-DMT on the body and common experiences from participants Current scientific research on this plant medicine The differences between natural and synthetic 5-MEO-DMT   Quotes: “This is one of the oldest psychedelics found in archeology.” [8:30] “Some kind of basic science studies on cell lines that showed that 5-MEO-DMT does in fact stimulate brain cell growth, antidepressant properties, and might boost the immune system.” [13:24] “In many psychedelics, you are getting formed visuals, you can kind of interact with what’s happening. This medicine kind of takes you beyond your biography, beyond your memory, beyond form into a very energetic experience.” [16:07] "80 percent, I think, of people that had this experience said it was profoundly sacred or holy." [28:48] “I also think that people don't need to be demonized that are using this medicine.” [51:31]   Links: Find Dr. Barsuglia Online MAPS Scientific Journals relating to this episode: Angel of human health: current research updates in toad medicine  The Development of Toad Toxins as Potential Therapeutic Agents New Therapeutic Aspects of Steroidal Cardiac Glycosides Pilot Study of Huachansu in Patients with Hepatocellular Carcinoma, Non-Small Cell Lung Cancer, or Pancreatic Cancer The Development of a Pharmacological Approach for Rabies Treatment Bufotenine is able to block rabies virus infection in BHK-21 cells Porangui

The Income Tax department (I-T) will impose a fine of Rs 10,000 on those who failing to link PAN card and Aadhaar card by March 31, 2020. Those PAN cards not linked to the Aadhaar will become inoperative. If the PAN card becomes inoperative, then the assessing officer shall levy a fine of Rs 10,000 under section 272 B of the I-T Act. One must note that ‘Inoperative PAN' means one will not be able to do several financial transactions where PAN is required. The IT department in the past had given multiple deadlines for linking PAN with Aadhaar and on every occasion, the deadlines were extended. Notably, the deadline for March 31, 2020, will not be extended further, as per an official press statement. The Assam Police along with security forces apprehended one ULFA(I) cadre and three linkmen in a joint operation at Chikrajan area in Bordumsa on mid-night hours in Tinsukia District. The apprehended ULFA(I) cadre was identified as Mridul Mahanta alias Nibir Asom, Linkmen Nirab Moran, Dipankar Moran and Sharot Moran. SM Group, the premier real estate builder based in Guwahati is ready with another mega project that will fulfill the aspirations of homebuyers. The well-known reality developer launched the residential project, Surya Aspire recently. The project will provide 1 BHK, 2 BHK, and 3 BHK affordable lifestyle homes to fulfill the growing demands in entire Northeast India. Indian Army at Se La in Arunachal Pradesh rescued 111 civilians & 70 vehicles stranded in snow at 14000 feet in sub zero temperature. The operation began on the night of 29 February and lasted until March 1. Those rescued thanked the Army profusely for saving their lives. Bollywood star Yami Gautam who was here in Guwahati to flag off the Great Guwahati Marathon 2020 (Night Marathon) on Sunday got trolled after a video of her arriving at the Guwahati LGBI Airport went viral. In the video, she was seen rejecting the Assamese Gamosa (Assamese scarf) which was offered to her by a fan. Meanwhile, the actor has given a clarification through her Twitter account saying that it was simply self-defense. Execution of convicts in Nirbhaya case has been deferred until further notice, announced a Delhi High Court. The convicts were about to be hanged on March 3 at 6 am. The decision was made as convict Pawan Kumar Gupta has filed a mercy plea before the President of India. The decision is still pending. Two people have tested positive for coronavirus in Delhi and Telangana. The one from Delhi had a travel history to Italy and the patient from Telangana had a travel history to Dubai. West Bengal Chief Minister Mamata Banerjee has said that Delhi riots were a “planned genocide”while addressing an event at Netaji Indoor Stadium in Kolkata she said. With four bodies discovered, the toll in Delhi is currently at 46. Lok Sabha was adjourned until Tuesday after a scuffle broke out between members of BJP and Congress.Members of both parties pushed and shoved each other.When the House met at 2 pm, members of opposition started shouting slogans from the well and went into the area of treasury benches. They wanted the resignation of Home Minister Amit Shah. The opposition party members were also carrying black banners.Members of BJP reacted to this and the entire episode spiraled out of control. The House was adjourned twice—once at 3 pm and then again at 4 pm. Members of opposition also tore papers in the house which was strongly condemned by the BJP. Parliamentary Affairs Minister Pralhad Joshi accused the opposition members for disrupting proceedings in the house. He also accused the Congress of anti-Sikh riots in 1984. Tata group's NELCO partnered with Panasonic Avionics had launched in-flight WiFi services in India. Passenger airline Vistara will be the first to provide such services to passengers.

Lilit Martirosyan misses the chance to go and sit somewhere outside to drink coffee. She hasn’t been able to do that for the past seven weeks. It’s too dangerous for her to even look out her own window.People can recognize her face now.She became the first transgender woman to speak to Armenia’s parliament last month. A video of her speech was shared around the world and applauded by LGBTQ activists and supporters. But she’s been getting death threats since then, and she’s afraid to leave the house.Armenia ranks as one of the worst countries to live in Europe for LGBTQ people, according to a new assessment this month by the International Lesbian, Gay, Bisexual, Trans and Intersex Association (ILGA-Europe). The post-Soviet republic came in near the bottom of the 49 countries listed, barely topping Azerbaijan and Turkey.At a parliamentary hearing held by the National Assembly in Yerevan on April 5, Martirosyan, the president of the Right Side nongovernmental organization, called for legal protection for transgender people in Armenia who often face harassment and violence.“I ask you to see me as a collective figure,” Martirosyan told Armenian lawmakers and activists. “I encompass in myself the tortured, raped, kidnapped, subjected to physical violence …  unemployed, poor and morally abandoned Armenian transgender’s image.”Martirosyan, herself, has been attacked multiple times on the streets of Yerevan. Three months ago, her teeth were broken and her head was badly injured, she told The World.She says the police didn’t do anything, and that she and her community don’t feel safe.In fact, her NGO tallied 283 crimes against transgender people who were “tortured, raped, kidnapped, subjected to physical violence, burned, immolated, knifed, subjected to murder attempts, killed” in Armenia from 2016 to 2018.Right Side, which claims to be the first and only organization that works to ensure protections for transgender people and sex workers in Armenia and in the South Caucasus, saw Martirosyan’s speech as a victory for themselves and the rest of the LGBTQ community in the country.But not everybody was celebrating.As soon as Martirosyan’s three-minute speech ended, Naira Zohrabyan, a member of parliament from the opposition Prosperous Armenia Party (BHK) who chaired the meeting, asked her to leave the hall.Zohrabyan called her speech “disrespectful,” adding that it had nothing to do with the agenda that day. The hearing was meant to cover issues of judicial reform and disability rights.A few days later, on April 8, there was backlash against Martirosyan.More than 100 supporters of nationalist and conservative groups led anti-LGBTQ protests in front of the National Assembly against Martirosyan and her speech, according to Radio Free Europe. A few parliamentarians verbally attacked her. Lilit Martirosyan Credit: Courtesy of the Right Side  One MP said Martirosyan should be burned in Republic Square, a popular center in the middle of the capital.Ever since then, Martirosyan and the Right Side have received dozens of death threats. The NGO closed down for a few weeks but reopened its doors on April 25.Following Martirosyan’s speech, in abusive videos posted online, “people dressed in military uniforms declared that they would find transgender activists and kill them. Local news media published home addresses for Ms. Martirosyan and her co-workers. Someone attached an Armenian flag to Ms. Martirosyan’s home,” The New York Times reported.Max Varzhapetyan, the community and office assistant at the Right Side, says they will continue to work because it’s their policy to be visible transgender activists.Varzhapetyan, who’s bigender and uses they/them pronouns, says that they and Martirosyan are particularly at risk since they were both at parliament that day. The only places they go are their homes and the NGO’s office to avoid harassment and violence, Varzhapetyan says, speaking on Martirosyan’s behalf as well.“The whole situation changed after the speech,” said Varzhapetyan, 23. “Because previously, people didn’t know who trans people were. Lilit’s speech delivered a powerful message. And even if people hate us and want to kill us, they learned about what Lilit [said].”Martirosyan started her activism at age 13. And by 15, she started taking birth control pills for estrogen because she says there are no other options for transgender women in Armenia. Today, she goes abroad to Ukraine for hormone replacement therapy pills.Doctors refuse to treat transgender people because of the discrimination, she says.It’s one of the issues her NGO is working to combat.In 2015, she became the first transgender woman in Armenia to legally change her name on her passport. The Right Side negotiated with the Civil Status Acts Registration Agency of the Staff of the Ministry of Justice to make the name change possible. Two years later, it became official policy in the country: Transgender people could legally change their names without having to provide psychological documentation or having three witnesses present, according to Varzhapetyan.But the organization says there’s still plenty of work to be done.A transgender person is eligible to change their passport only if they’ve undergone sex reassignment surgery in Armenia.Although Martirosyan had the surgery, which was performed by a Russian doctor, she has chosen to keep her sex as “male” on her passport. She says she won’t officially change her gender to match her identity until every transgender person in Armenia has the right to do so, with or without sex reassignment surgery.)Martirosyan and the Right Side have largely received international support. The United Nations office in Yerevan and the European Union both released statements condemning violence against the LGBTQ community in Armenia.But MP Zohrabyan says the whole country was shocked by Martirosyan’s presentation on transgender rights.“We need time for that,” Zohrabyan said in Russian. “Our church is not ready for this conversation.”The state-financed Armenian Apostolic Church is believed to be the world’s oldest Christian institution. It’s widely seen as a major part of the country’s national identity. After Martirosyan’s speech, a priest burned incense to sanctify the area where she spoke, reported OC Media, a news outlet that covers the Caucasus region.“Our society is completely different,” Zohrabyan said. “It’s not that of Holland, Austria or France. We need to accept our mentality the way it is.”Although Zohrabyan was widely criticized for her reaction to Martirosyan, which some called discriminatory, BHK officials wrote a statement defending the MP.The country’s peaceful transition to government last year, known as the “Velvet Revolution,” spurred hope for a new Armenia. For the LGBTQ community, too.Armenian Prime Minister Nikol Pashinyan “publicly denounced any threats of violence and hate speech” on “numerous occasions,” a spokesman said in an email to The New York Times.But many activists say that not much has changed for the community under Pashinyan, who was swept to power by the street protests.Brett Burnham, a gay man who moved from the US to Yerevan in August 2018 to work as a public health professor at the American University of Armenia (AUA), blames the lack of dialogue around these issues.“So, this idea that Armenians can't change or that this is just a patriarchal society might be true for now because people aren't willing to have these conversations, and the fact is — and there's data lots of data to support this — that LGBTQ people leave this country. They don't want to stay here they don't feel supported.”According to a 2015 report by Pink Armenia, an LGBTQ rights group, 5,891 citizens left Armenia due to discrimination based on gender and sexuality between 2011 and 2013 alone.At AUA, Burnham sensed a need for a safe space for gay, lesbian, transgender students allies on campus. That’s what led him to start a group under conditions by the school’s administration that he keep it anonymous. He would have to name it “International Students Meeting,” he said.Students would meet informally and share coming out stories, said Burnham, who researches cancer and LGBTQ health disparities in Armenia and beyond.  “These students are deeply traumatized,” he said.“I knew there was homophobia in Armenia, but I didn’t know the extent to which it existed,” said Burnham, 35. “I was a little surprised. But then when I met the students, it was clear that they were petrified.”He didn’t expect to be harassed himself. Especially not by his own students over email and social media.“I have been verbally attacked and attacked via email by students viciously, and there's no consequence for them because of my sexuality and the research that I do,” he said.In his course evaluations, one student wrote that “Dr. Burnham performed great as an instructor. In contrast, he is an extremely aggressive homosexual’s advocate.” Another student said, “this is discrimination of students, because we are imposed to tolerate something that [we] don’t want to invade our environment.”Half a year into his tenure at the public health department, he was asked to leave and never come back by AUA, a university accredited by WASC Senior College and University Commission in California. His annual contract wasn’t renewed. Burnham says he doesn’t know why. He wasn’t given a reason. He suspects it might be because of his sexuality.Yeva Margaryan, one of Burnham’s advisees in the public health department, said she hasn't witnessed any harassment by the administration. But, she said, it is likely many students didn’t understand why he was “talking openly about his sexuality.”“There are a lot of [gay people] in Armenia, but not very many are open,” Margaryan, 23, said. “I just think it’s about our mentality. It’s Armenia. And our culture is like that. I just want [Burnham] to understand that he has nothing to do with that.”AUA responded to The World’s request for comment and said that it has a “non-discrimination policy in its hiring practices,” and that “Dr. Burnham offered information about his sexual orientation. However, that information was inconsequential in the hiring decision.”The statement continues, “Dr. Burnham signed a one-year contract. The decision not to renew his contract was made in accordance to the Armenian law and considering several factors, including the needs of the program and student feedback. Obviously, personnel matters are confidential and to protect the rights of the employee and the employer cannot be shared with third parties. But we assure you that Dr. Burnham’s sexual orientation had no effect on the decision not to renew his contract.”Burnham, who is currently suing the university over an intellectual property issue, says that he feels exhausted, but “duty-bound,” to his students at AUA who respect him and his work. He’s going to finish grading papers until the end of the semester. He says he’s glad that this chapter of his life is almost over.As he packs his bags to leave Armenia next month, Martirosyan is more determined than ever to stay in her country and continue fighting for LGBTQ rights there.Editor’s note: Lilit Martirosyan is no relation to Lucy Martirosyan, who authored this story. This story has been updated to further clarify what harassment Yeva Margaryan witnessed. 

Lilit Martirosyan misses the chance to go and sit somewhere outside to drink coffee. She hasn't been able to do that for the past seven weeks. It's too dangerous for her to even look out her own window.People can recognize her face now.She became the first transgender woman to speak to Armenia's parliament last month. A video of her speech was shared around the world and applauded by LGBTQ activists and supporters. But she's been getting death threats since then, and she's afraid to leave the house.Armenia ranks as one of the worst countries to live in Europe for LGBTQ people, according to a new assessment this month by the International Lesbian, Gay, Bisexual, Trans and Intersex Association (ILGA-Europe). The post-Soviet republic came in near the bottom of the 49 countries listed, barely topping Azerbaijan and Turkey.At a parliamentary hearing held by the National Assembly in Yerevan on April 5, Martirosyan, the president of the Right Side nongovernmental organization, called for legal protection for transgender people in Armenia who often face harassment and violence.“I ask you to see me as a collective figure,” Martirosyan told Armenian lawmakers and activists. “I encompass in myself the tortured, raped, kidnapped, subjected to physical violence …  unemployed, poor and morally abandoned Armenian transgender's image.”Martirosyan, herself, has been attacked multiple times on the streets of Yerevan. Three months ago, her teeth were broken and her head was badly injured, she told The World.She says the police didn't do anything, and that she and her community don't feel safe.In fact, her NGO tallied 283 crimes against transgender people who were “tortured, raped, kidnapped, subjected to physical violence, burned, immolated, knifed, subjected to murder attempts, killed” in Armenia from 2016 to 2018.Right Side, which claims to be the first and only organization that works to ensure protections for transgender people and sex workers in Armenia and in the South Caucasus, saw Martirosyan's speech as a victory for themselves and the rest of the LGBTQ community in the country.But not everybody was celebrating.As soon as Martirosyan's three-minute speech ended, Naira Zohrabyan, a member of parliament from the opposition Prosperous Armenia Party (BHK) who chaired the meeting, asked her to leave the hall.Zohrabyan called her speech “disrespectful,” adding that it had nothing to do with the agenda that day. The hearing was meant to cover issues of judicial reform and disability rights.A few days later, on April 8, there was backlash against Martirosyan.More than 100 supporters of nationalist and conservative groups led anti-LGBTQ protests in front of the National Assembly against Martirosyan and her speech, according to Radio Free Europe. A few parliamentarians verbally attacked her. Lilit Martirosyan Credit: Courtesy of the Right Side  One MP said Martirosyan should be burned in Republic Square, a popular center in the middle of the capital.Ever since then, Martirosyan and the Right Side have received dozens of death threats. The NGO closed down for a few weeks but reopened its doors on April 25.Following Martirosyan's speech, in abusive videos posted online, “people dressed in military uniforms declared that they would find transgender activists and kill them. Local news media published home addresses for Ms. Martirosyan and her co-workers. Someone attached an Armenian flag to Ms. Martirosyan's home,” The New York Times reported.Max Varzhapetyan, the community and office assistant at the Right Side, says they will continue to work because it's their policy to be visible transgender activists.Varzhapetyan, who's bigender and uses they/them pronouns, says that they and Martirosyan are particularly at risk since they were both at parliament that day. The only places they go are their homes and the NGO's office to avoid harassment and violence, Varzhapetyan says, speaking on Martirosyan's behalf as well.“The whole situation changed after the speech,” said Varzhapetyan, 23. “Because previously, people didn't know who trans people were. Lilit's speech delivered a powerful message. And even if people hate us and want to kill us, they learned about what Lilit [said].”Martirosyan started her activism at age 13. And by 15, she started taking birth control pills for estrogen because she says there are no other options for transgender women in Armenia. Today, she goes abroad to Ukraine for hormone replacement therapy pills.Doctors refuse to treat transgender people because of the discrimination, she says.It's one of the issues her NGO is working to combat.In 2015, she became the first transgender woman in Armenia to legally change her name on her passport. The Right Side negotiated with the Civil Status Acts Registration Agency of the Staff of the Ministry of Justice to make the name change possible. Two years later, it became official policy in the country: Transgender people could legally change their names without having to provide psychological documentation or having three witnesses present, according to Varzhapetyan.But the organization says there's still plenty of work to be done.A transgender person is eligible to change their passport only if they've undergone sex reassignment surgery in Armenia.Although Martirosyan had the surgery, which was performed by a Russian doctor, she has chosen to keep her sex as “male” on her passport. She says she won't officially change her gender to match her identity until every transgender person in Armenia has the right to do so, with or without sex reassignment surgery.)Martirosyan and the Right Side have largely received international support. The United Nations office in Yerevan and the European Union both released statements condemning violence against the LGBTQ community in Armenia.But MP Zohrabyan says the whole country was shocked by Martirosyan's presentation on transgender rights.“We need time for that,” Zohrabyan said in Russian. “Our church is not ready for this conversation.”The state-financed Armenian Apostolic Church is believed to be the world's oldest Christian institution. It's widely seen as a major part of the country's national identity. After Martirosyan's speech, a priest burned incense to sanctify the area where she spoke, reported OC Media, a news outlet that covers the Caucasus region.“Our society is completely different,” Zohrabyan said. “It's not that of Holland, Austria or France. We need to accept our mentality the way it is.”Although Zohrabyan was widely criticized for her reaction to Martirosyan, which some called discriminatory, BHK officials wrote a statement defending the MP.The country's peaceful transition to government last year, known as the “Velvet Revolution,” spurred hope for a new Armenia. For the LGBTQ community, too.Armenian Prime Minister Nikol Pashinyan “publicly denounced any threats of violence and hate speech” on “numerous occasions,” a spokesman said in an email to The New York Times.But many activists say that not much has changed for the community under Pashinyan, who was swept to power by the street protests.Brett Burnham, a gay man who moved from the US to Yerevan in August 2018 to work as a public health professor at the American University of Armenia (AUA), blames the lack of dialogue around these issues.“So, this idea that Armenians can't change or that this is just a patriarchal society might be true for now because people aren't willing to have these conversations, and the fact is — and there's data lots of data to support this — that LGBTQ people leave this country. They don't want to stay here they don't feel supported.”According to a 2015 report by Pink Armenia, an LGBTQ rights group, 5,891 citizens left Armenia due to discrimination based on gender and sexuality between 2011 and 2013 alone.At AUA, Burnham sensed a need for a safe space for gay, lesbian, transgender students allies on campus. That's what led him to start a group under conditions by the school's administration that he keep it anonymous. He would have to name it “International Students Meeting,” he said.Students would meet informally and share coming out stories, said Burnham, who researches cancer and LGBTQ health disparities in Armenia and beyond.  “These students are deeply traumatized,” he said.“I knew there was homophobia in Armenia, but I didn't know the extent to which it existed,” said Burnham, 35. “I was a little surprised. But then when I met the students, it was clear that they were petrified.”He didn't expect to be harassed himself. Especially not by his own students over email and social media.“I have been verbally attacked and attacked via email by students viciously, and there's no consequence for them because of my sexuality and the research that I do,” he said.In his course evaluations, one student wrote that “Dr. Burnham performed great as an instructor. In contrast, he is an extremely aggressive homosexual's advocate.” Another student said, “this is discrimination of students, because we are imposed to tolerate something that [we] don't want to invade our environment.”Half a year into his tenure at the public health department, he was asked to leave and never come back by AUA, a university accredited by WASC Senior College and University Commission in California. His annual contract wasn't renewed. Burnham says he doesn't know why. He wasn't given a reason. He suspects it might be because of his sexuality.Yeva Margaryan, one of Burnham's advisees in the public health department, said she hasn't witnessed any harassment by the administration. But, she said, it is likely many students didn't understand why he was “talking openly about his sexuality.”“There are a lot of [gay people] in Armenia, but not very many are open,” Margaryan, 23, said. “I just think it's about our mentality. It's Armenia. And our culture is like that. I just want [Burnham] to understand that he has nothing to do with that.”AUA responded to The World's request for comment and said that it has a “non-discrimination policy in its hiring practices,” and that “Dr. Burnham offered information about his sexual orientation. However, that information was inconsequential in the hiring decision.”The statement continues, “Dr. Burnham signed a one-year contract. The decision not to renew his contract was made in accordance to the Armenian law and considering several factors, including the needs of the program and student feedback. Obviously, personnel matters are confidential and to protect the rights of the employee and the employer cannot be shared with third parties. But we assure you that Dr. Burnham's sexual orientation had no effect on the decision not to renew his contract.”Burnham, who is currently suing the university over an intellectual property issue, says that he feels exhausted, but “duty-bound,” to his students at AUA who respect him and his work. He's going to finish grading papers until the end of the semester. He says he's glad that this chapter of his life is almost over.As he packs his bags to leave Armenia next month, Martirosyan is more determined than ever to stay in her country and continue fighting for LGBTQ rights there.Editor's note: Lilit Martirosyan is no relation to Lucy Martirosyan, who authored this story. This story has been updated to further clarify what harassment Yeva Margaryan witnessed. 

Episode: No. 7 - Motor Milestones in Children from Birth to 1 Year Guest(s): Kate Heays, MScPT  and Laura Patrick, BHK, MScPT   Today Genevieve and Heather are welcoming Kate Heays and Laura Patrick from Kids Physio Group.   The conversation will include:    • What are Motor Milestones and when should they be achieved.  • Techniques to help your child achieve the milestones.  • The importance of “Tummy Time”.  • What to do if your child is not achieving their milestones.

Prions have been extensively studied since they represent a new class of infectious agents in which a protein, PrPSc (prion scrapie), appears to be the sole component of the infectious particle. They are responsible for transmissible spongiform encephalopathies (TSEs), which affect both, humans and animals. Human prion diseases occur in infectious, sporadic or genetic forms. The "protein only" hypothesis argues that the key event in the pathogenesis represents the conversion of the normal host protein, PrPc, into its pathogenic isoform PrPSc. Prion diseases have been associated with the accumulation of this abnormally folded protein and its neurotoxic effects. However, it is not known if PrPc loss of function is an important factor since the normal biological function of PrPc, a cell surface-anchored glycoprotein predominantly expressed in neuronal cells, and the cellular processes in which this protein is involved remain obscure. Recently, the human 37 kDa laminin receptor precursor (LRP), which represents the precursor of the human 67 kDa high-affinity laminin receptor (LR), was identified as a binding partner for the cellular prion protein in a yeast two-hybrid screen. In order to characterize the possible role of LRP/LR as a cell surface receptor for PrPc, cell culture studies were performed to investigate the cellular localization of PrP and LRP/LR and to analyse the binding and internalization behaviour of PrP depending on the presence of LRP/LR on the cell surface of neuronal and non-neuronal cells. Immunofluorescence analysis of non-permeabilized murine neuroblastoma cells demonstrated that PrP and LRP/LR co-localize on the surface of these cells. In addition, baby hamster kidney (BHK) cells transfected with recombinant Semlik-Forest virus RNAs overexpressed human PrP and human LRP at their cell surface, the latter one orientated as a type II transmembrane protein with its C-terminus outside and its N-terminus inside the cell. Co-localization of both proteins was observed on BHK cells co-transfected with LRP and PrP encoding recombinant SFV RNAs. Cell binding and internalization assays with recombinant human PrP demonstrated the LRP/LR-dependent binding and endocytosis of externally added human PrP. An increased, dose-dependent cell binding of recombinant PrP was demonstrated by BHK cells overexpressing full-length human LRP on their cell surface. Trypsin treatment of the cell surface revealed the LRP dependent internalization of GST-tagged and untagged, glycosylated PrP. In contrast to wild-type LRP, the expression of an LRP mutant lacking its transmembrane domain led to the secretion of this mutant from transfected BHK cells and totally abolished the binding and internalization of exogenous, recombinant PrP. This LRP mutant could function as a decoy recetor in therapy of TSEs. The strict LRP/LR specificity of the PrP binding to neuronal cells was verified by testing the displacement capacity of a series of different antibodies in the LRP-PrP binding reaction. Only LRP and PrP specific antibodies were able to block totally the binding of human GST-fused PrP to N2a and NT2 cells whereas various control antibodies used for competition showed no effect. Mapping analyses in the yeast two-hybrid system and cell-binding assays identified direct and heparan sulfate proteoglycan (HSPG)-dependent interaction sites mediating the binding of cellular PrP to the 37-kDa/67-kDa LRP/LR. The relationship between the 37-kDa LRP and the 67-kDa high-affinity LR is unknown so far. Both forms were observed in plasma membrane fractions of N2a cells. We conclude from these data that the 37-kDa/67-kDa laminin receptor acts as the main cell surface receptor for PrP. High-level expression and purification of recombinant, glycosylated prion proteins in mammalian cells is essential for a better understanding of the physiological function of PrPc and biochemical processes responsible for prion diseases. Due to the presence of important organelles, membranes and other cellular cofactors which are necessary for the correct processing, trafficking and localization of prion proteins mammalian cell culture systems such as the Semliki-Forest virus (SFV) system allow the synthesis and characterization of wild-type as well as mutant PrP to get a better insight into the biology of these proteins. Therefore, the SFV system was used to generate recombinant highly glycosylated human wild-type and human disease-associated mutant prion proteins as well as FLAG-tagged human and bovine PrP in cultured BHK cells. Both mutated variants, which are related to the human prion diseases fatal familial insomnia (FFI) and Creutzfeldt-Jakob disease (CJD) reveal proteinase K (PK) resistance, one of the most typical biochemical properties characteristic for the infectious scrapie isoform of the prion protein. The subcellular location of both PrP mutants at the cell surface and in intracellular compartments of transfected BHK cells was similar to that of wild-type PrP without any significant differences regarding the cellular distribution and expression level. In addition, FLAG-tagged prion proteins were expressed with high efficiency in BHK cells showing the typical glycosylation pattern allowing the rapid and simple purification via anti-FLAG antibody chromatography. PrP dimers could play an essential role in the PrPc to PrPSc conversion process and might be involved in PrP interspecies transmission. Recently, crystallization of the prion protein in a dimeric form was reported. Size exclusion chromatography showed that native soluble homogeneous FLAG tagged prion proteins from hamster, man and cattle expressed in the baculovirus system were predominantly dimeric. The PrP/PrP interaction was confirmed in rec. SFV-RNA transfected BHK cells co-expressing FLAG and oligohistidine tagged human PrP. The yeast two-hybrid system identified the octarepeat region and the C-terminal structured domain (aa90-aa230) of PrP as PrP/PrP interaction domains. The identification of the 37-kDa/67-kDa laminin receptor as the receptor for the cellular prion protein might represent an important step for a better understanding of the molecular biology of prion diseases and might lead to the development of powerful therapeutics such as LRP/LR specific antibodies for the treatment of these unconventional diseases.

Cell mediated immunity to vaccinia virus in man was studied by lymphocyte transformation. Vaccinia antigen, propagated on BHK-21 and Vero cells, could be used successfully for in-vitro testing after partial purification as well as crude infectious homogenates. Vaccinia antigen preparations were effective both in the infective and the inactivated state. Inactivation was usually accompanied with a certain loss of stimulating activity. Development of cell mediated immune response in-vitro after first vaccination was investigated in 17 adults. Vaccinia virus specific lymphocyte transformation was seen in the second week after vaccination in all cases. Following revaccination no increase of lymphocyte transformation ratio could be observed in 11 persons studied. At the same time the titers of humoral antibodies were elevated.