Podcasts about pluripotent

Pluripotent stem cells are defined, in part, by their potential to generate cell types from all three embryonic germ layers. However, it is well known within the field that there is variability in developmental potential between cell lines. This phenomenon, sometimes referred to as lineage bias, is manifest in a variable response of individual cell lines to induction of differentiation into a specific germ layer lineage. Although lineage bias in pluripotent stem cells has been reported for some years, we do not fully understand its molecular basis, or its implications for normal development. The guests on today's program studied functional variation in human PSC lines as they progress through neurectoderm versus mesendoderm lineages and fore- versus hind-brain development. They will discuss the origin and consequences of inter-individual variation in the early events orchestrating human neural development, the extent to which such variation might impact on adult health and disease, and how it might be exploited in stem cell therapy. GuestsRon McKay is the Director of Basic Science at the Lieber Institute and has appointments in multiple departments and the McKusick-Nathans Institute of Genetic Medicine at the Johns Hopkins School of Medicine, USA. He currently serves as a member of the Stem Cell Reports Editorial Board. Suel-Kee Kim is an Associate Research Scientist in the Department of Neuroscience at Yale University School of Medicine, USA. Seungmae Seo is an Assistant Professor at University of Maryland Eastern Shore, USA. Seungmae is a former ISSCR Merit and Travel Award Winner. HostMartin Pera, Editor-in-Chief, Stem Cell Reports and The Jackson Laboratory@martinperaJAXSupporting ContentIndividual variation in the emergence of anterior-to-posterior neural fates from human pluripotent stem cells, Stem Cell ReportsAbout Stem Cell ReportsStem Cell Reports is the open access, peer-reviewed journal of the International Society for Stem Cell Research (ISSCR) for communicating basic discoveries in stem cell research, in addition to translational and clinical studies. Stem Cell Reports focuses on original research with conceptual or practical advances that are of broad interest to stem cell biologists and clinicians.X: @StemCellReportsAbout ISSCRWith nearly 5,000 members from more than 80 countries, the International Society for Stem Cell Research (@ISSCR) is the preeminent global, cross-disciplinary, science-based organization dedicated to stem cell research and its translation to the clinic. The ISSCR mission is to promote excellence in stem cell science and applications to human health.ISSCR StaffKeith Alm, Chief Executive OfficerYvonne Fisher, Managing Editor, Stem Cell ReportsKym Kilbourne, Director of Media and Strategic CommunicationsJack Mosher, Scientific AdvisorVoice WorkBen Snitkoff

Benjamin Freedman, Ph.D., delves into the creation and functionality of kidney organoids. He discusses their focus on filtration over hormone functions, and answer intriguing questions about urine characterization and the necessity of organoid structures. Freedman explores the use of scaffolds for implants and ponder the philosophical implications of off-target cells in organoids, questioning whether these cells are a natural part of organ development. Series: "Stem Cell Channel" [Health and Medicine] [Science] [Show ID: 39455]

Benjamin Freedman, Ph.D., delves into the creation and functionality of kidney organoids. He discusses their focus on filtration over hormone functions, and answer intriguing questions about urine characterization and the necessity of organoid structures. Freedman explores the use of scaffolds for implants and ponder the philosophical implications of off-target cells in organoids, questioning whether these cells are a natural part of organ development. Series: "Stem Cell Channel" [Health and Medicine] [Science] [Show ID: 39455]

Benjamin Freedman, Ph.D., delves into the creation and functionality of kidney organoids. He discusses their focus on filtration over hormone functions, and answer intriguing questions about urine characterization and the necessity of organoid structures. Freedman explores the use of scaffolds for implants and ponder the philosophical implications of off-target cells in organoids, questioning whether these cells are a natural part of organ development. Series: "Stem Cell Channel" [Health and Medicine] [Science] [Show ID: 39455]

Benjamin Freedman, Ph.D., delves into the creation and functionality of kidney organoids. He discusses their focus on filtration over hormone functions, and answer intriguing questions about urine characterization and the necessity of organoid structures. Freedman explores the use of scaffolds for implants and ponder the philosophical implications of off-target cells in organoids, questioning whether these cells are a natural part of organ development. Series: "Stem Cell Channel" [Health and Medicine] [Science] [Show ID: 39455]

Benjamin Freedman, Ph.D., delves into the creation and functionality of kidney organoids. He discusses their focus on filtration over hormone functions, and answer intriguing questions about urine characterization and the necessity of organoid structures. Freedman explores the use of scaffolds for implants and ponder the philosophical implications of off-target cells in organoids, questioning whether these cells are a natural part of organ development. Series: "Stem Cell Channel" [Health and Medicine] [Science] [Show ID: 39455]

Benjamin Freedman, Ph.D., delves into the creation and functionality of kidney organoids. He discusses their focus on filtration over hormone functions, and answer intriguing questions about urine characterization and the necessity of organoid structures. Freedman explores the use of scaffolds for implants and ponder the philosophical implications of off-target cells in organoids, questioning whether these cells are a natural part of organ development. Series: "Stem Cell Channel" [Health and Medicine] [Science] [Show ID: 39455]

Sherry Ning is an author and an essayist, whose Substack, "Pluripotent," has some of the best writing I've come across on human nature, purpose, and how to live. During our conversation, Shelly talks about creating one's own path, success, open-mindedness, beauty, nostalgia, spirituality, and religion.------------Support via VenmoSupport on SubstackSupport on Patreon------------Rate on SpotifyRate on Apple PodcastsSocial media and all episodes------------00:00 Intro00:36 A purpose to write 2:37 Trait openness 6:12 Real diversity is found in human personality 8:37 Fame 10:29 WEIRD people 12:45 For happiness, ask: what will make me miserable? 15:47 Success and going your own way 26:36 Open-mindedness and particularity of taste 34:06 Wildflowers: beauty and toughness 39:52 The death of Sherry's father43:27 Nostalgia and good times 49:49 What modern writer does Sherry enjoy? 52:26 Sherry's religious and spiritual beliefs

Toshihiro Kobayashi, Ph.D., presents his research on the mechanisms driving cell fate choices in early mammalian embryos. He also discusses the potential application of these principles in future regenerative medicine. Series: "Stem Cell Channel" [Health and Medicine] [Science] [Show ID: 38328]

Toshihiro Kobayashi, Ph.D., presents his research on the mechanisms driving cell fate choices in early mammalian embryos. He also discusses the potential application of these principles in future regenerative medicine. Series: "Stem Cell Channel" [Health and Medicine] [Science] [Show ID: 38328]

Toshihiro Kobayashi, Ph.D., presents his research on the mechanisms driving cell fate choices in early mammalian embryos. He also discusses the potential application of these principles in future regenerative medicine. Series: "Stem Cell Channel" [Health and Medicine] [Science] [Show ID: 38328]

Toshihiro Kobayashi, Ph.D., presents his research on the mechanisms driving cell fate choices in early mammalian embryos. He also discusses the potential application of these principles in future regenerative medicine. Series: "Stem Cell Channel" [Health and Medicine] [Science] [Show ID: 38328]

Toshihiro Kobayashi, Ph.D., presents his research on the mechanisms driving cell fate choices in early mammalian embryos. He also discusses the potential application of these principles in future regenerative medicine. Series: "Stem Cell Channel" [Health and Medicine] [Science] [Show ID: 38328]

Toshihiro Kobayashi, Ph.D., presents his research on the mechanisms driving cell fate choices in early mammalian embryos. He also discusses the potential application of these principles in future regenerative medicine. Series: "Stem Cell Channel" [Health and Medicine] [Science] [Show ID: 38328]

Toshihiro Kobayashi, Ph.D., presents his research on the mechanisms driving cell fate choices in early mammalian embryos. He also discusses the potential application of these principles in future regenerative medicine. Series: "Stem Cell Channel" [Health and Medicine] [Science] [Show ID: 38328]

Buck discusses Yamanaka Factors and recent experiments where a team led by David Sinclair was successful in reversing cellular age applying the concepts in an experiment. 00:00:02 - Is it possible to stop or even reverse the aging process? 00:01:48 - 2012 research by Shinya Yamanaka - Yamanaka Factors  00:02:50 - Pluripotent stem cells 00:03:08 - Revolutionary discovery for the world of regenerative medicine  00:03:28 - Salk Institute study using mice with progeria 00:03:55 - Doxycycline and improvements in signs of aging 00:04:26 - David Sinclair's research to regenerate optic nerves in mice to restore vision 00:04:49 - David Sinclair's book on aging research called Lifespan 00:05:20 - Epigenome and the aging process 00:06:15 - Horvath lab and using Yamanaka factors to reset epigenetic clocks in rats

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2023.07.31.551374v1?rss=1 Authors: Ishioka, M., Nihashi, Y., Sunagawa, Y., Umezawa, K., Shimosato, T., Kagami, H., Morimoto, T., Takaya, T. Abstract: An 18-base myogenetic oligodeoxynucleotide (myoDN), iSN04, acts an anti-nucleolin aptamer and induces myogenic differentiation of skeletal muscle myoblasts. This study investigated the effect of iSN04 on murine embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs). In the undifferentiated state, iSN04 inhibited the proliferation of ESCs and iPSCs but did not affect the expression of pluripotent markers. In the differentiating condition, iSN04 treatment of ESCs/iPSCs from day 5 onward dramatically induced the differentiation into Nkx2-5+ beating cardiomyocytes with upregulation of Gata4, Isl1, and Nkx2-5, whereas iSN04 treatment from earlier stages completely inhibited cardiomyogenesis. RNA sequencing revealed that iSN04 treatment from day 5 onward contributes to the generation of cardiac progenitors by modulating the Wnt signaling pathway. Immunostaining showed that iSN04 suppressed the cytoplasmic translocation of nucleolin and restricted it to the nucleoli. These results demonstrate that nucleolin inhibition by iSN04 facilitates the terminal differentiation of cardiac mesoderm into cardiomyocytes, but interferes with the differentiation of early mesoderm into the cardiac lineage. This is the first report on the generation of cardiomyocytes from pluripotent stem cells using a DNA aptamer. Since iSN04 did not induce hypertrophic responses in primary-cultured cardiomyocytes, iSN04 would be useful and safe for the regenerative therapy of heart failure using stem cell-derived cardiomyocytes. Copy rights belong to original authors. Visit the link for more info Podcast created by Paper Player, LLC

"Invest in yourself and upskill" & "At 25 yrs, if you have spent 5 years in MBBS do continue medicine, it has a great future ahead" - Dr Hakikat . Dr HPM discusses a lot of things and questions with  Dr Hakikat Bir. Dr Hakikat Bir is a clinical AI, digital health specialist and a practicing physician.  Medical Director at Birmingham, Alabama based XpertDox, he is a recipient of the prestigious "Designing and Implementing AI Solutions for Healthcare” Prize - awarded by the Associate Dean, Harvard Medical School Executive Education. And has been a part of the finalist team at MIT Healthcare Innovation Bootcamp, 2022. Dr. Hakikat holds a Professional Degree in Medicine and Surgery from a government medical college in Kolkata, and is currently pursuing a post-graduate diploma in computer science and artificial intelligence from a Delhi - State University. Both Dr Hakikat Bir and Dr Hara Prasad are colleagues and in recent past have worked on couple of health-tech projects. The Points that are disscussed in this podcast includes :- 1. How was MBBS overally for Dr Hakikat ? 2. What pulled towards AI? 3. Learning about AI 4. Coding on regular basis 5. Post MBBS life 6. Experience of working in ITBP 7. Experience of Harvard and MIT 8. Getting into current role? 9.Do you think medical curriculum should encourage electives and AI should be a part of curriculum 10.ChatGPT in healthcare? 11. How to upskill? 12.Msg for medicos to think out of box If anyone wants to connect to Dr Hakikat Bir, you can connect on linkedin - (https://www.linkedin.com/in/drhbsb) .

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2023.04.20.537628v1?rss=1 Authors: Wang, X., Zhang, W., Zhao, S., Yan, H., Xin, Z., Cui, T., Zang, R., Zhao, L., Wang, H., Zhou, J., Li, X., Yue, W., Xi, J., Zhang, Z., Fang, X., Pei, X. Abstract: Ex vivo RBC production generates unsatisfactory expansion, {beta}-globin expression, and maturation of erythroid cells. The underlying mechanisms behind these limitations and ex vivo terminal erythropoiesis from different origins are largely unexplained. In this study, we mapped an atlas of ex vivo terminally differentiated cells from umbilical cord blood mononuclear cells (UCBMNs) and pluripotent stem cells (PSCs), and observed the differential regulatory dynamics of erythropoiesis from these two origins at a single-cell resolution. We detected the presence of hematopoietic stem progenitor cells (HSPCs), erythroid progenitor (e.g., CFU-E), and non-erythroid cells (e.g., macrophages) in the terminal populations. We observed that UCBMN-derived erythropoiesis is more active than PSC-derived erythropoiesis in terms of the cell cycle, stress erythropoiesis, and autophagy at single cell resolution, which may provide new insights into the limitations in cell expansion, globin expression, and maturation in ex vivo RBC production, respectively. We verified that a stress-erythropoiesis-related gene, TRIB3, increases the expression of globin genes in ex vivo erythropoiesis. As the major unexpected component detected in terminally differentiated cells, CFU-E were further characterized as having high- or low-expansion capacity based on CD99 expression, which generally decreased over erythropoiesis. By inhibiting CD99 gene expression using antagonists, we increased reticulocyte production in the population. Heterogeneous CFU-Es also exist in bone marrow. Moreover, decreased CD99 expression mediates the interactions between macrophages and CFU-E during ex vivo erythropoiesis. Overall, our results provide a reference for facilitating the development of strategies to improve ex vivo RBC regeneration. Copy rights belong to original authors. Visit the link for more info Podcast created by Paper Player, LLC

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2023.03.22.533592v1?rss=1 Authors: Yu, X., Yuan, H., Yang, Y., Zheng, W., Zheng, X., Lu, S.-H., Jiang, W., Yu, X. Abstract: Self-renewing, damage-repair and differentiation of mammalian stratified squamous epithelia are subject to tissue homeostasis, but the regulation mechanisms remain elusive. Here, we investigate the esophageal squamous epithelial tissue homeostasis in vitro and in vivo. We establish a rat esophageal organoid (rEO) in vitro system and show that the landscapes of rEO formation, development and maturation trajectories can mimic those of rat esophageal epithelia in vivo. Single-cell RNA sequencing (scRNA-seq), snap-shot immunostaining and functional analyses of stratified "matured" rEOs define that the epithelial pluripotent stem-cell determinants, p63 and Sox2, play crucial but distinctive roles for regulating mammalian esophageal tissue homeostasis. We identify two cell populations, p63+Sox2+ and p63-Sox2+, of which the p63+Sox2+ population presented at the basal layer is the cells of origin required for esophageal epithelial stemness maintenance and proliferation whereas the p63-Sox2+ population presented at the suprabasal layers is the cells of origin having a dual role for esophageal epithelial differentiation (differentiation-prone fate) and rapid tissue damage-repair responses (proliferation-prone fate). Given the fact that p63 and Sox2 are developmental lineage oncogenes and commonly overexpressed in ESCC tissues, p63-Sox2+ population could not be detected in organoids formed by esophageal squamous cell carcinoma (ESCC) cell lines. Taken together, these findings reveal that the tissue homeostasis is maintained distinctively by p63 and/or Sox2 dependent cell lineage populations required for the tissue renewing, damage-repair and protection of carcinogenesis in mammalian esophagi. Copy rights belong to original authors. Visit the link for more info Podcast created by Paper Player, LLC

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2023.03.03.530736v1?rss=1 Authors: Kobori, C., Takagi, R., Yokomizo, R., Yoshihara, S., Mori, M., Takahashi, H., Javaregowda, P. K., Akiyama, T., Ko, M. S. H., Kishi, K., UMEZAWA, A. Abstract: Background: Melanocytes are an essential part of the epidermis, and their regeneration has received much attention because propagation of human adult melanocytes in vitro is too slow for clinical use. Differentiation from human pluripotent stem cells to melanocytes has been reported, but the protocols to produce them require multiple and complex differentiation steps. Method: We differentiated human embryonic stem cells (hESCs) that transiently express JMJD3 to pigmented cells. We investigated whether the pigmented cells have melanocytic characteristics and functions by qRT-PCR, immunocytochemical analysis and flow cytometry. We also investigated their biocompatibility by injecting the cells into immunodeficient mice for clinical use. Result: We successfully differentiated and established a pure culture of melanocytes. The melanocytes maintained their growth rate for a long time, approximately 200 days, and were functional. They exhibited melanogenesis and transfer of melanin to peripheral keratinocytes. Moreover, melanocytes simulated the developmental processes from melanoblasts to melanocytes. The melanocytes had high engraftability and biocompatibility in the immunodeficient mice. Conclusion: The robust generation of functional and long-lived melanocytes are key to developing clinical applications for the treatment of pigmentary skin disorders. Copy rights belong to original authors. Visit the link for more info Podcast created by Paper Player, LLC

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2023.03.03.530824v1?rss=1 Authors: Liu, C. Z., Prasad, A., Jadhav, B., Sharp, A. J., Gelb, B. D. Abstract: Valvular heart disease presents a significant health burden, yet advancements in valve biology and novel therapeutics have been hindered by the lack of accessibility to human valve cells. In this study, we have developed a scalable and feeder-free method to differentiate human induced pluripotent stem cells (iPSCs) into endocardial cells. Importantly, we show that these endocardial cells are transcriptionally and phenotypically distinct from vascular endothelial cells and can be directed to undergo endothelial-to-mesenchymal transition (EndMT) to generate cardiac valve cell populations. Following this, we identified two distinct populations--one population undergoes EndMT to become valvular interstitial cells (VICs), while the other population reinforces their endothelial identity to become valvular endothelial cells (VECs). Lastly, we confirmed the identities of our iPSC-derived cell populations and identified putative markers through transcriptomic analyses. By increasing the accessibility to these cell populations, we aim to accelerate discoveries for cardiac valve biology and disease. Copy rights belong to original authors. Visit the link for more info Podcast created by Paper Player, LLC

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2023.02.24.529793v1?rss=1 Authors: Song, X., Guo, P., Wang, M., Chen, L., Zhang, J., Xu, M., Liu, N., Liu, M., Fang, L., Xu, X., Gu, Y., Xia, K., Li, B. Abstract: Callus is a reprogrammed transitional cell mass during plant regeneration. Pluripotent callus cells develop into fertile shoots through de novo shoot organogenesis. This study represents a pioneering effort in exploring the spatial transcriptome of tomato callus during shoot regeneration, using technologies including BGI Stereo-seq, BMKMANU S1000, and 10x Visium. The results indicate that the callus comprises highly heterogeneous cells, classified into various cell types based on spatial gene expression and histological observation, including epidermis, shoot primordium, vascular tissue, inner callus, and outgrowth shoots. The developmental trajectories from shoot primordium to outgrowth shoot are traced, and vascular tissue development is characterized. The single-cell resolution spatial approach reveals the origin of shoot primordia from the sub-epidermis. The spatial full length RNA sequencing shows high incompletely spliced (IS) ratios in the shoot primordium cells. These findings enhance our knowledge of plant organogenesis and highlight the significance of spatial biology in plant research. Copy rights belong to original authors. Visit the link for more info Podcast created by Paper Player, LLC

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2023.01.30.526265v1?rss=1 Authors: Snoeck, H.-W., Matkovic Leko, I., Schrode, N., Pezet, M. G., Thimraj, T. A., Beaumont, K., Torres, J. A. Abstract: Several novel distal lung populations were recently identified that may be involved in regeneration after injury. As these are absent in rodents, a deeper understanding of their roles and lineage relations requires availability of equivalent cells in vitro. Here we report the generation of expandable, clonal spheres, called transitional lung organoids (TLOs), from human pluripotent stem cells. TLOs consist mainly of previously identified type 0 alveolar epithelial (AT0) cells, terminal respiratory bronchiole stem cells and distal basal cells (BCs). Velocity analysis of single cell RNAseq data suggests that distal BCs are the most undifferentiated progenitors in the TLOs and give rise to AT0 cells. TLOs will be an important resource for studies in human lung regeneration, and potentially for regenerative approaches for human lung disease. Copy rights belong to original authors. Visit the link for more info Podcast created by Paper Player, LLC

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2023.01.19.524655v1?rss=1 Authors: Burgess, C. L., Huang, J., Bawa, P., Alysandratos, K.-D., Minakin, K., Morley, M. P., Babu, A., Villacorta-Martin, C., Hinds, A., Thapa, B. R., Wang, F., Matschulat, A. M., Morrisey, E. E., Varelas, X., Kotton, D. N. Abstract: In the distal lung, alveolar epithelial type I cells (AT1s) comprise the vast majority of alveolar surface area and are uniquely flattened to allow the diffusion of oxygen into the capillaries. This structure along with a quiescent, terminally differentiated phenotype has made AT1s particularly challenging to isolate or maintain in cell culture. As a result, there is a lack of established models for the study of human AT1 biology, and in contrast to alveolar epithelial type II cells (AT2s), little is known about the mechanisms regulating their differentiation. Here we engineer a human in vitro AT1 model system through the directed differentiation of induced pluripotent stem cells (iPSC). We first define the global transcriptomes of primary adult human AT1s, suggesting gene-set benchmarks and pathways, such as Hippo-LATS-YAP/TAZ signaling, that are enriched in these cells. Next, we generate iPSC-derived AT2s (iAT2s) and find that activating nuclear YAP signaling is sufficient to promote a broad transcriptomic shift from AT2 to AT1 gene programs. The resulting cells express a molecular, morphologic, and functional phenotype reminiscent of human AT1 cells, including the capacity to form a flat epithelial barrier which produces characteristic extracellular matrix molecules and secreted ligands. Our results indicate a role for Hippo-LATS-YAP signaling in the differentiation of human AT1s and demonstrate the generation of viable AT1-like cells from iAT2s, providing an in vitro model of human alveolar epithelial differentiation and a potential source of human AT1s that until now have been challenging to viably obtain from patients. Copy rights belong to original authors. Visit the link for more info Podcast created by Paper Player, LLC

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2023.01.08.523148v1?rss=1 Authors: Marzec-Schmidt, K., Ghosheh, N., Stahlschmidt, S. R., Kuppers-Munther, B., Synnergren, J., Ulfenborg, B. Abstract: Revolutionary advances in AI and deep learning in recent years have resulted in an upsurge of papers exploring applications within the biomedical field. Within stem cell research, promising results have been reported from analyses of microscopy images to e.g., distinguish between pluripotent stem cells and differentiated cell types derived from stem cells. In this work, we investigated the possibility of using a deep learning model to predict the differentiation stage of pluripotent stem cells undergoing differentiation towards hepatocytes, based on morphological features of cell cultures. We were able to achieve close to perfect classification of images from early and late time points during differentiation, and this aligned very well with the experimental validation of cell identity and function. Our results suggest that deep learning models can distinguish between different cell morphologies, and provide alternative means of semi-automated functional characterization of stem cell cultures. Copy rights belong to original authors. Visit the link for more info Podcast created by Paper Player, LLC

https://psychiatry.dev/wp-content/uploads/speaker/post-11000.mp3?cb=1669703456.mp3 Playback speed: 0.8x 1x 1.3x 1.6x 2x Download: Current progress in understanding schizophrenia using genomics and pluripotent stem cells: A meta-analytical overview – Ashwani Choudhary et al. Schizophrenia Research. 2022.Full EntryCurrent progress in understanding schizophrenia using genomics and pluripotent stem cells: A meta-analytical overview –

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2022.11.14.516407v1?rss=1 Authors: Shin, K. C., Ali, G., Ali Moussa, H. Y., Gupta, V., de la Fuente, A., Kim, H.-G., Stanton, L., Park, Y. Abstract: Autism spectrum disorder (ASD) is a complex and heterogeneous neurodevelopmental disorder linked to numerous rare, inherited and arising de novo genetic variants. ASD often co-occurs with attention-deficit hyperactivity disorder and epilepsy, which are associated with hyperexcitability of neurons. However, the physiological and molecular mechanisms underlying hyperexcitability in ASD remain poorly understood. Transient receptor potential canonical-6 (TRPC6) is a Ca2+-permeable cation channel that regulates store-operated calcium entry (SOCE) and is a candidate risk gene for ASD. Using human pluripotent stem cell (hPSC)-derived cortical neurons, single cell calcium imaging, and electrophysiological recording, we show that TRPC6 knockout (KO) reduces SOCE signaling and leads to hyperexcitability of neurons by increasing action potential frequency and network burst frequency. Our data provide evidence that reduction of SOCE by TRPC6 KO results in neuronal hyperexcitability, which we hypothesize is an important contributor to the cellular pathophysiology underlying hyperactivity in some ASD. Copy rights belong to original authors. Visit the link for more info Podcast created by Paper Player, LLC

FOREBORN - very sweet; he is totally desirable. This is my beloved! This is my companion, O maidens of Jerusalem! It is never worth a first-class man's time to express a majority opinion. By definition, there are plenty of others to do that. Snow, C. P. (1967). Foreword. A Mathematician's Apology. By Hardy, G. H. Cambridge University Press. Then Moses went up on the mountain, and the cloud covered the mountain. The glory of the Lord dwelt on Mount Sinai, and the cloud covered it six days. And on the seventh day he called to Moses out of the midst of the cloud. Exodus 24:15-16 He was still speaking when, behold, a bright cloud overshadowed them, and a voice from the cloud said, “This is my beloved Son, with whom I am well pleased; listen to him.” Matthew 17:5 And a cloud overshadowed them, and a voice came out of the cloud, “This is my beloved Son; listen to him.” Mark 9:7 And a voice came out of the cloud, saying, “This is my Son, my Chosen One; listen to him!” Luke 9:35 For when he received honor and glory from God the Father, and the voice was borne to him by the Majestic Glory, “This is my beloved Son, with whom I am well pleased,” 2Peter 1:17 1 Corinthians 15:8 ἔσχατον δὲ πάντων ὡσπερεὶ τῷ ἐκτρώματι ὤφθη κἀμοί. Last of all, as to one untimely born, he appeared also to me. One born out of due time—Greek, “the one abortively born”: the abortion in the family of the apostles. As a child born before the due time is puny, and though born alive, yet not of the proper size, and scarcely worthy of the name of man, so “I am the least of the apostles,” scarcely “meet to be called an apostle”; a supernumerary (early 17th century: from late Latin supernumerarius ‘(soldier) added to a legion after it is complete', from Latin super numerum ‘beyond the number'. present in excess of the normal or requisite number. (of a person) not belonging to a regular staff but engaged for extra work. not wanted or needed; redundant. "books were obviously supernumerary, and he began jettisoning them" (of an actor) appearing on stage but not speaking. taken into the college of apostles out of regular course, not led to Christ by long instruction, like a natural birth, but by a sudden power, as those prematurely born [Grotius]. Compare the similar image from childbirth, and by the same spiritual power, the resurrection of Christ (1 Pe 1:3). “Begotten again by the resurrection of Jesus.” Jesus' appearance to Paul, on the way to Damascus, is the one here referred to. Robert Jamieson, A. R. Fausset, and David Brown, Commentary Critical and Explanatory on the Whole Bible, vol. 2 (Oak Harbor, WA: Logos Research Systems, Inc., 1997), 292. Pluripotent stem cells have unique potential in research and therapy because by definition they have a number of special properties: 1. They have no predetermined program, they are a blankslate 2. They can self-renew indefinitely 3. They can give rise to all the tissues of the body J Exp Clin Med. Author manuscript; available in PMC 2012 Mar 9. Published in final edited form as: J Exp Clin Med. 2010 Oct 22; 2(5): 202–217. doi: 10.1016/S1878-3317(10)60033-2 PMCID: PMC3298413 NIHMSID: NIHMS356426 PMID: 22408700 George T.-J. Huang* George T.-J. Huang, Department of Endodontics, Boston University School of Dental Medicine, Boston, Massachusetts, USA; * Corresponding author. Department of Endodontics, Boston University School of Dental Medicine, 100 E. Newton Street, Boston, MA 02118, USA. ude.ub@gnauhjtg Glorification | The Final Frontier To Boldly go where only one Man has Gone Before! Decrease time over target:  PayPal or Venmo @clastronaut Cash App $clastronaut

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2022.09.07.507028v1?rss=1 Authors: Berryer, M. H., Tegtmeyer, M., Binan, L., Valakh, V., Nathanson, A., Trendafilova, D., Crouse, E., Klein, J., Meyer, D., Pietilainen, O., Rapino, F., Farhi, S., Rubin, L. L., McCarroll, S., Nehme, R., Barrett, L. E. Abstract: Astrocytes play essential roles in normal brain function, with dysfunction implicated in diverse developmental and degenerative disease processes. Emerging evidence of profound species divergent features of astrocytes coupled with the relative inaccessibility of human brain tissue underscore the utility of human pluripotent stem cell (hPSC) technologies for the generation and study of human astrocytes. However, existing approaches for hPSC-astrocyte generation are typically lengthy, incompletely characterized, or require intermediate purification steps, limiting their utility for multi-cell line, adequately powered functional studies. Here, we establish a rapid and highly scalable method for generating functional human induced astrocytes (hiAs) based upon transient Neurogenin 2 (NGN2) induction of neural progenitor-like cells followed by maturation in astrocyte media, which demonstrate remarkable homogeneity within the population and across 11 independent cell lines in the absence of additional purification steps. These hiAs express canonical astrocyte markers, respond to pro-inflammatory stimuli, exhibit ATP-induced calcium transients and support neuronal maturation in vitro. Moreover, single-cell transcriptomic analyses reveal the generation of highly reproducible cell populations across individual donors, most closely resembling human fetal astrocytes, and highly similar to hPSC-derived astrocytes generated using more complex approaches. Finally, the hiAs capture key molecular hallmarks in a trisomy 21 disease model. Thus, hiAs provide a valuable and practical resource well-suited for study of basic human astrocyte function and dysfunction in disease. Copy rights belong to original authors. Visit the link for more info Podcast created by PaperPlayer

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2022.08.25.505166v1?rss=1 Authors: Lendemeijer, B., Unkel, M., Mossink, B., Hijazi, S., Sampedro, S. G., Shpak, G., Slump, D. E., van den Hout, M. C. G. N., van IJcken, W. F. J., Bindels, E. M. J., Hoogendijk, W. J. G., Nadif Kasri, N., de Vrij, F. M. S., Kushner, S. A. Abstract: Astrocytes are essential for the formation and maintenance of neural networks through metabolic support, facilitation of synaptic function, and optimization of electrophysiological activity. However, a major technical challenge for investigating astrocyte function and disease-related pathophysiology has been the limited ability to obtain functional human astrocytes. Here we present a novel method to efficiently differentiate human pluripotent stem cell (hPSC)-derived neural progenitors to functional astrocytes in 28 days using a culture medium containing leukemia inhibitory factor (LIF) and bone morphogenetic protein 4 (BMP4). This approach yields highly pure populations of astrocytes expressing canonical astrocyte markers, which we confirmed by immunofluorescence, flow cytometry and RNA sequencing. Human PSC-derived astrocytes efficiently buffer glutamate and robustly support neural network activity. Co-cultures of hPSC-derived astrocytes and neurons on multi-electrode arrays generated robust network activity within 2 days and synchronous network bursts after 6 days. Whole cell patch-clamp recordings revealed an increased frequency of postsynaptic currents in human hPSC-derived neurons co-cultured with hPSC-derived versus primary rodent astrocytes, consistent with a corresponding increase in synapse density. Furthermore, hPSC-derived astrocytes retained their hominid morphology when transplanted into a mouse brain. In conclusion, we present a novel protocol to obtain functional astrocytes from human pluripotent stem cells, providing a platform for investigating human astrocyte function and neuronal-glial interactions. Copy rights belong to original authors. Visit the link for more info Podcast created by PaperPlayer

Thorold Theunissen, Ph.D., of the Washington University School of Medicine in St. Louis shares his work using naive stem cells to model trophoblast development, Series: "Stem Cell Channel" [Health and Medicine] [Science] [Show ID: 37674]

Thorold Theunissen, Ph.D., of the Washington University School of Medicine in St. Louis shares his work using naive stem cells to model trophoblast development, Series: "Stem Cell Channel" [Health and Medicine] [Science] [Show ID: 37674]

Thorold Theunissen, Ph.D., of the Washington University School of Medicine in St. Louis shares his work using naive stem cells to model trophoblast development, Series: "Stem Cell Channel" [Health and Medicine] [Science] [Show ID: 37674]

Thorold Theunissen, Ph.D., of the Washington University School of Medicine in St. Louis shares his work using naive stem cells to model trophoblast development, Series: "Stem Cell Channel" [Health and Medicine] [Science] [Show ID: 37674]

Thorold Theunissen, Ph.D., of the Washington University School of Medicine in St. Louis shares his work using naive stem cells to model trophoblast development, Series: "Stem Cell Channel" [Health and Medicine] [Science] [Show ID: 37674]

Thorold Theunissen, Ph.D., of the Washington University School of Medicine in St. Louis shares his work using naive stem cells to model trophoblast development, Series: "Stem Cell Channel" [Health and Medicine] [Science] [Show ID: 37674]

Thorold Theunissen, Ph.D., of the Washington University School of Medicine in St. Louis shares his work using naive stem cells to model trophoblast development, Series: "Stem Cell Channel" [Health and Medicine] [Science] [Show ID: 37674]

A medic by trade, Mark Kotter has always held a particular interest in neuroscience and stem cell research.As founder and CEO of bit.bio, he's overseen the development of a unique approach to "reprogram" stem cells and change them into consistent and scalable disease-relevant cells, which can then be screened for potential therapeutics to help treat such diseases as Huntington's Alzheimer's, and ALS.Hear about this revolutionary technology and how it works, what benefits it can provide researchers, and how it could very well shape the future of drug development.Show Notes  Stem Cells for Drug Discovery  Building a Stable of Stem Cells  Conversations in Science With Disruptors Leading the Way  Neuroscience | Charles River  bit.bio  Resources  https://www.bit.bio/news/focus-on-a-founder-with-mark-kotter-celebrating-10-years-of-community  https://www.criver.com/eureka/podcast/e28-building-the-building-blocks-of-life   https://www.criver.com/about-us/disruptors/meet-mark?region=3601  https://www.bit.bio/  Stem Cells for Drug Discovery  Next Generation Reprogramming of Human iPSCs to Generate Disease-specific Glutamatergic Neurons Carrying HTT Gene for Huntington's Disease Research and Drug Discovery https://www.criver.com/neuroscience-2021 

40 percent of feeling in movies and television is done through music. It is often used to amplify the chemistry between actors or the importance of a scene. And it can also conceal production flaws or poor performances.What's it like to be a composer in the Entertainment World? Today's very special guest is accomplished Canadian composer Christopher Nickel.I had the pleasure of working with him on a short film called Pluripotent in what feels like a lifetime ago. He has since then performed concerts all over the world and composed music for movies and shows on networks such as Discovery and Hallmark Channel.As many soundtracks are becoming more of a song album, I believe through this conversation you will appreciate the artistry behind the work of greatly trained musicians.Christopher shares with me the challenges of his love for classic music and the promotion of his work through solo albums and performing. He also gives us great insights on cultivating patience.Let's all discover a greater sense of connection with the beauty of the world through notes.Connect with Christopher Tyler Nickel:WebsiteIMDbMusic Sample 1Music Sample 2Let's Connect!Alexia Melocchi - WebsiteLittle Studio Films - WebsiteTwitterInstagramFacebookLinkedIn

This episode of BioTech IQ features Kleanthis Xanthopoulos, Ph.D. He is the co-founder, chairman of the board, and CEO of Shoreline Biosciences. As part of our "What's Ahead in Biotech: #2022" series Kleanthis talks about the power of induced pluripotent stem cells (iPSC) derived NK cells,  and macrophage immunotherapies. What is the future of immunotherapies? Listen and find out!https://shorelinebio.com/https://thebiotechiqpodcast.com/

Chuck Murry, MD, PhD shares recent research on using cardiomyocytes to model COVID-19 heart disease. He also discusses current efforts to regenerate the heart and reduce arrhythmias. Series: "Stem Cell Channel" [Health and Medicine] [Science] [Show ID: 37016]

Chuck Murry, MD, PhD shares recent research on using cardiomyocytes to model COVID-19 heart disease. He also discusses current efforts to regenerate the heart and reduce arrhythmias. Series: "Stem Cell Channel" [Health and Medicine] [Science] [Show ID: 37016]

Chuck Murry, MD, PhD shares recent research on using cardiomyocytes to model COVID-19 heart disease. He also discusses current efforts to regenerate the heart and reduce arrhythmias. Series: "Stem Cell Channel" [Health and Medicine] [Science] [Show ID: 37016]

Chuck Murry, MD, PhD shares recent research on using cardiomyocytes to model COVID-19 heart disease. He also discusses current efforts to regenerate the heart and reduce arrhythmias. Series: "Stem Cell Channel" [Health and Medicine] [Science] [Show ID: 37016]

Chuck Murry, MD, PhD shares recent research on using cardiomyocytes to model COVID-19 heart disease. He also discusses current efforts to regenerate the heart and reduce arrhythmias. Series: "Stem Cell Channel" [Health and Medicine] [Science] [Show ID: 37016]

Chuck Murry, MD, PhD shares recent research on using cardiomyocytes to model COVID-19 heart disease. He also discusses current efforts to regenerate the heart and reduce arrhythmias. Series: "Stem Cell Channel" [Health and Medicine] [Science] [Show ID: 37016]

Chuck Murry, MD, PhD shares recent research on using cardiomyocytes to model COVID-19 heart disease. He also discusses current efforts to regenerate the heart and reduce arrhythmias. Series: "Stem Cell Channel" [Health and Medicine] [Science] [Show ID: 37016]

Chuck Murry, MD, PhD shares recent research on using cardiomyocytes to model COVID-19 heart disease. He also discusses current efforts to regenerate the heart and reduce arrhythmias. Series: "Stem Cell Channel" [Health and Medicine] [Science] [Show ID: 37016]

Hi you guys! We have a new episode of #TheDopeScienceShowPodcast. I had the wonderful opportunity to chat with neuroscientist Yewande. We talk about her love for house music and most recently Afrobeats. She curates a monthly playlist on soundcloud called No Wahala. We discuss our favorite dance spots in LA and what inspired her to move from London to LA on a whim. What motivated her to get a PHD in Neuroscience and her research with mini brains/organoids to test medical treatments models. IG- nice_forthememoirs Topics: --Transplanting mini brains into mice to see how organoids develop inside a living organism. Will it affect the mice's behavior? --She teaches me about cell development. Different cell types: Stem cells versus embryotic cell versus Pluripotent stem cells- (an immature or stem cell) capable of giving rise to several different cell types. --What is the difference between RNA and DNA? --- Send in a voice message: https://anchor.fm/the-dope-science-show/message