Podcasts about high content

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2023.07.06.547985v1?rss=1 Authors: Migliori, B., Bose, N., Paull, D. Abstract: High-content imaging (HCI) is a popular technique that leverages high throughput datasets to uncover phenotypes of cell populations in vitro. When the differences between populations (such as a healthy and disease state) are completely unknown, it is crucial to build very large HCI screens to account for individual (donor) variation, as well as having enough replicates to create a reliable model. One approach to highlight phenotypic differences is to reduce images into a set of features using unbiased methods, such as embeddings or autoencoders. These methods are powerful at preserving the predictive power contained in each image while removing most of the unimportant image features and noise (e.g., background). However, they do not provide interpretable information about the features driving the decision process of the AI algorithm used. While tools have been developed to address this issue, such as CellProfiler, scaling this tool to large sample batches containing hundreds of thousands of images poses computational challenges. Additionally, the resulting feature vector, computationally expensive to have generated, is very large in size (containing over 3000 features) with many redundant features, making it challenging to perform further analysis and identify the truly relevant features. Ultimately, there is an increased risk of overfitting due to the presence of too many non-meaningful features that can ultimately skew downstream predictions. To address this issue, we have developed ScaleFExSM, a Python pipeline that extracts multiple generic fixed features at the single cell level that can be deployed across large high-content imaging datasets with low computational requirements. This pipeline efficiently and reliably computes features related to shape, size, intensity, texture, granularity as well as correlations between channels. Additionally, it allows the measurement of additional features specifically related to mitochondria and RNA only, as they represent important channels with characteristics worth to be measured on their own. The measured features can be used to not only separate populations of cells using AI tools, but also highlight the specific interpretable features that differ between populations. We applied ScaleFExSM to identify the phenotypic shifts that multiple cell lines undergo when exposed to different compounds. We used a combination of recursive feature elimination, logistic regression, correlation analysis and dimensionality reduction representations to narrow down to the most meaningful features that described the drug shifts. Furthermore, we used the best scoring features to extract images of cells for each class closest to the average to visually highlight the phenotypic shifts caused by the drugs. Using this approach, we were able to identify features linked to the drug shifts in line with literature, and we could visually validate their involvement in the morphological changes of the cells. ScaleFExSM can be used as a powerful tool to understand the underlying phenotypes of complex diseases and subtle drug shifts at the single cell level, bringing us a step closer to identifying disease-modifying compounds for the major diseases of our time. Copy rights belong to original authors. Visit the link for more info Podcast created by Paper Player, LLC

I went to SLAS this spring to talk to old friends, meet some new ones and learn whatever I could. I met Maggie Vantangoli, the Director of Marketing at Araceli Biosciences in their booth where we chatted about the use of artificial intelligence (AI) for high content analysis (HCA) in drug discovery and development. She was kind enough to join me on this podcast and fill in the (large) holes in my knowledge of cell biology. HCA is basically automated microscopy and analysis of fluorescently-stained (or otherwise) cells to identify changes caused by various compounds. This rich phenotypic information can provide valuable insights into how a drug or compound might work or whether it could be toxic.You can listen to the podcast to learn about how HCA is used to group compounds based on the morphological changes in cells exposed to them. A few things stood out for me. In terms of AI, the most interesting application was that of unsupervised learning, where the AI can learn to detect patterns that a human might not look for or even see if they were looking. One can imagine that a lot of information about biology could be uncovered aside from evaluating potential therapies.When I asked about where HCA might go in the future, Maggie talked about doing tests on organoids as a replacement for animal models. This is a topic that has come up in a few of my conversations lately on and off this podcast.I also asked Maggie what she thought about the possibilities for combining HCA with DoE (Design of Experiments) that I featured a few episodes back. Thankfully, she listened to that episode. (You should too.)I definitely think there are amazing applications here and you know, I think it's a massive amount of untapped potential, particularly in that high content space.I know that different aspects of drug discovery are getting there, particularly like the structure-activity type relationships. But I think this is a completely untapped market for the DoE type of type of work and there's huge amounts of applications because high content's also, you know, per assay, like most assays in drug discovery, there's cost associated there and that's not... When you're scaling up, that is not in insignificant amounts of money. So running millions or even tens of thousands of compounds a day with staining and all the liquid handling and tips and all of that is, is quite expensive. The amount put into that one little plate is extremely expensive.So you can kind of do your work ahead of time and, and utilize the Design of Experiments to stack the deck in your favor. Sorry to interrupt here, but you are subscribed aren't you?I get a special joy out of when two episodes connect in that way. I'm going to see if we can get Maggie to come back with Markus to discuss the possibilities.I'd love to hear what you think of this episode or the podcast overall. Feel free to drop a comment.Also, if you want to talk about custom content for your life science brand, let's chat. This is a public episode. If you would like to discuss this with other subscribers or get access to bonus episodes, visit cclifescience.substack.com

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2023.03.24.534108v1?rss=1 Authors: Hoffstadt, J. G., Wotring, J. W., Porter, S., Halligan, B. S., O'Meara, M. J., Tai, A. W., Sexton, J. Z. Abstract: Dengue Virus (DENV) causes dengue fever, a pandemic-potential disease with currently no FDA-approved antivirals. Additionally, the available vaccine for DENV can increase the risk of severe dengue fever for those who have never had a DENV infection due to antibody-dependent enhancements. Thus, there is an urgent need to identify dengue virus antivirals. Antivirals that target NS4B, the replication compartment forming protein of DENV and the flavivirus family, are a promising new drug class that minimize cytotoxic effects to host cells. Drug-repurposing and high-content screening were leveraged to efficiently identify antivirals likely to inhibit NS4B. Using high-content screening, we quantified the morphological patterns of NS4B and envelope (E) protein expression versus time and developed a viral pseudotime model that was able to predict the infection progression to enable drug screening. We then developed a single-cell infection classifier for antiviral efficacy and performed high-throughput drug screening of 960 compounds. We identified four concentration-dependent inhibitors of DENV with nanomolar potencies including: Nexium, Pralatrexate, GW4064, and LY411575. LY411575, a gamma secretase inhibitor, exhibited an IC50 of 72nM and reduced percent infection to levels indistinguishable from the mock infection control. Copy rights belong to original authors. Visit the link for more info Podcast created by Paper Player, LLC

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2023.03.01.530592v1?rss=1 Authors: Wong, C. H., Wingett, S., Qian, C., Taliaferro, M., Ross-Thriepland, D., Bullock, S. L. Abstract: The cytoplasmic dynein-1 (dynein) motor plays a key role in cellular organisation by transporting a wide variety of cellular constituents towards the minus ends of microtubules. However, relatively little is known about how the biosynthesis, assembly and functional diversity of the motor is orchestrated. To address this issue, we have conducted an arrayed CRISPR loss-of-function screen in human cells using the distribution of dynein-tethered peroxisomes and early endosomes as readouts. From a guide RNA library targeting 18,253 genes, 195 validated hits were recovered and parsed into those impacting multiple dynein cargoes and those whose effects are restricted to a subset of cargoes. Clustering of high-dimensional phenotypic fingerprints generated from multiplexed images revealed co-functional genes involved in many cellular processes, including several candidate novel regulators of core dynein functions. Mechanistic analysis of one of these proteins, the RNA-binding protein SUGP1, provides evidence that it promotes cargo trafficking by sustaining functional expression of the dynein activator LIS1. Our dataset represents a rich source of new hypotheses for investigating microtubule-based transport, as well as several other aspects of cellular organisation that were captured by our high-content imaging. Copy rights belong to original authors. Visit the link for more info Podcast created by Paper Player, LLC

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2023.01.26.525784v1?rss=1 Authors: Tsubouchi, A., An, Y., Kawamura, Y., Yanagihashi, Y., Murata, Y., Teranishi, K., Ishiguro, S., Aburatani, H., Yachie, N., Ota, S. Abstract: Fast enrichment of cells based on morphological information remains a challenge, limiting genome-wide perturbation screening for diverse high-content phenotypes of cells. Here we show that multi-modal ghost cytometry-based cell sorting is applicable to fast pooled CRISPR screening for both fluorescence and label-free high-content phenotypes of millions of cells. By employing the high-content cell sorter in the fluorescence mode, we enabled the genome-wide CRISPR screening of genes that affect NF-{kappa}B nuclear translocation. Furthermore, by employing the multi-parametric, label-free mode, we performed the large-scale screening to identify a gene involved in macrophage polarization. Especially the label-free platform can enrich target phenotypes without invasive staining, preserving untouched cells for downstream assays and unlocking the potential to screen for the cellular phenotypes even when suitable markers are lacking. Copy rights belong to original authors. Visit the link for more info Podcast created by Paper Player, LLC

This is the latest episode of the free DDW podcast, “Finding the right cancer drug with high-content screening”. It covers an article by the same name written for Volume 23, Issue 1 – Winter 2021/22 of DDW. In the article, Lassi Paavolainen, Principal Investigator at the Institute for Molecular Medicine Finland (FIMM), Vilja Pietiäinen, Adjunct Professor in Cell and Molecular Biology, and Amanda Jones, Life Science Research Strategy Leader at PerkinElmer explain how functional precision medicine using ex vivo drug testing and HCS on patient-derived 3D cancer cell cultures shows promise for improving individual's treatment journey.

In this episode, we're joined by Broad Institute Senior Group Leader and Head of the Cimini Lab Imaging Platform Beth Cimini, Ph.D., for a lesson in high-content image analysis! Cimini is a subject matter expert who shares her knowledge to explain how high-content image analysis works and how it pertains to cell painting. Key Learning Points:The types of research high-content imaging is ideal for The process of developing an assay for a high-content imaging approachWhat you need to know to use high-content imaging in your research About Beth Cimini, Ph.D.Cimini is a senior group leader, CZI imaging scientist and the head of the Cimini Lab in the Imaging Platform at the Broad Institute in Cambridge, MA. After completing her undergraduate research in visual neuroscience at Boston University, Cimini obtained a Ph.D. in Biochemistry and Molecular Biology at the University of California San Francisco, where she studied the difference between splicing variants of the telomere master scaffolding protein TIN2.  She created and directs the Platform's Postdoctoral Training Program in Bioimage Analysis, and also leads the Broad efforts toward community engagement and driving biological projects for the Center for Open Bioimage Analysis.Stay connected with SLAS:Online at www.slas.orgFacebookTwitter @SLAS_OrgLinkedInInstagram @slas_orgYouTubeAbout SLAS:SLAS (Society for Laboratory Automation and Screening) is an international professional society of academic, industry and government life sciences researchers and the developers and providers of laboratory automation technology. The SLAS mission is to bring together researchers in academia, industry and government to advance life sciences discovery and technology via education, knowledge exchange and global community building.  For more information about SLAS, visit www.slas.org.SLAS publishes two peer-reviewed and MEDLINE-indexed scientific journals, SLAS Discovery and SLAS Technology. For more information about SLAS and its journals, visit www.slas.org/publications.For Students:Visit the SLAS Student Resources to find information on student-focused SLAS awards, grants, scholarships, digital educational content, podcasts and more.Upcoming SLAS Events: SLAS2023 International Conference and Exhibition February 25 - March 1, 2023 San Diego, CA, USA SLAS 2023 Building Biology in 3D Symposium 20-21 April 2023 Cambridge, United Kingdom SLAS Europe 2023 Conference and Exhibition 23-26 May 2023 Brussels, Belgium

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2022.11.18.516878v1?rss=1 Authors: Hu, J., Serra-Picamal, X., Bakker, G.-J., Troys, M. V., Winograd-katz, S., Ege, N., Gong, X., Didan, Y., Grosheva, I., Polansky, O., Bakkali, K., Hamme, E. V., Erp, M. v., Vullings, M., Weiss, F., Clucas, J., Dowbaj, A. M., Sahai, E., Ampe, C., Geiger, B., Friedl, P., Bottai, M., Stromblad, S. Abstract: High-content image-based cell phenotyping provides fundamental insights in a broad variety of life science areas. Striving for accurate conclusions and meaningful impact demands high reproducibility standards, even more importantly with the advent of data sharing initiatives. However, the sources and degree of biological and technical variability, and thus the reproducibility and usefulness of meta-analysis of results from live-cell microscopy have not been systematically investigated. Here, using high content data describing features of cell migration and morphology, we determine the sources of variability across different scales, including between laboratories, persons, experiments, technical repeats, cells and time points. Significant technical variability occurred between laboratories, providing low value to direct meta-analysis on the data from different laboratories. However, batch effect removal markedly improved the possibility to combine image-based datasets of perturbation experiments. Thus, reproducible quantitative high-content cell image data and meta-analysis depend on standardized procedures and batch correction applied to studies of perturbation effects. Copy rights belong to original authors. Visit the link for more info Podcast created by Paper Player, LLC

Jeffrey is trying to make sense of a medical emergency. Brett experiences a paradigm shift in how he thinks about his bipolar diagnosis. Christina talks about the unique challenges of live broadcasts.

Enrico Lugli, Humanitas Milano, Italy speaks on "High content flow cytometry to dissect memory T cell responses". This video is part of Course “Flow cytometry: from basic principles to advanced applications-CytoFlow@ICGEB”, an online event 28-30 September 2021. For more informations please visit https://www.icgeb.org.

Du siehst die Welt nicht wie sie ist, sondern wie Du bist & das immer durch die Brille Deiner Glaubenssätze! Wie siehst Du das Leben? Ist es für Dich ein Kampf, ein Ponyhof oder doch auch ein Spiel das sich vor allem zu gewinnen lohnt? Kennst Du schon die Spielregeln des Lebens ohne die Du nicht gewinnen kannst? Bereit für ungebremste Motivation & High Content in diesem Energy Talk?! Denn Du bist nicht Schuld an der Programmierung die Du bekommen hast, aber es ist Deine Verantwortung diese zu ändern, wenn Dir Dein aktueller Umstand nicht gefällt oder Du mit Deinem Mindset nicht das Leben erreichst, welches Du Dir eigentlich tief im Herzen wünschst & Du vor allem verdient hast! Und glaub mir, es wird nicht einfach, aber es wird sich verdammt nochmal lohnen, doch dafür musst Du eine Entscheidung treffen! Dich weiterzubilden, Dich Deinem Erfolg & dem Leben Deiner Träume unterzustellen & alles in Deiner Macht stehende dafür tun, damit Du Deine Ziele auch nachhaltig erreichst! Du musst die Ursache schaffen & sein von der Wirkung die Du letztendlich haben möchtest! Und wenn Du da noch Support brauchst & vor allem lernen möchtest wie Du die mächtigste Instanz Deines Lebens, welche immerhin 95 - 99% steuert, ein für alle mal programmierst, dann trag Dich Jetzt zu einem kostenlosen Mastermindcall ein & lass Dich von faszinierendem Wissen, atemberaubenden Techniken & unendlicher Energie überschütten & das ganze für ein kleines Feedback. Also sicher Dir Jetzt einen Platz zu einem Dir passenden Zeitpunkt & fang an noch heute die Grundlage für das Leben Deiner Träume zu erschaffen! https://linktr.ee/houseofpassion "Die Definition von Wahnsinn lautet, alles beim alten zu belassen & zu hoffen, dass sich etwas ändert." Albert Einstein Also wenn Du andere Ergebnisse möchtest, wie z. B. ein freies & Selbstbestimmtes Leben, dann kommst Du an der Macht des Unterbewusstseins nicht vorbei, also kneif die Arschbacken zusammen & mach Dich endlich auf die Reise zum Leben Deiner Träume!!! We change the World ~Together Angefangen bei Deiner! ~ Der Wahrscheinlich Nachhaltigste Coach der Welt ~

Heute mit einem absoluten Specialguest & zwar dem Potenzialentfalter Marco Eichinger. Wahnsinnig was er in seinen jungen Jahren schon alles auf die Beine gestellt hat & noch faszinierender ist das was er macht & wie er 1/3 Deiner ganzen Lebenszeit & zwar die Schlafenszeit mit luzidem Träumen & maximaler Potenzialentfaltung für Dich nutzbar macht. Lass Dich inspirieren von diesem High Content & lass uns gerne Deine Meinung zu unserem aktuellen Schulsystem & was Du persönlich verbessern würdest da. Also wenn Du den guten Marco mal kennen lernen möchtest & glaub mir, dass solltest Du & ist seine Zeit wert, dann findest Du ihn hier. Gratis Erstgespräch: calendly.com/eichingermarco Instagram: https://instagram.com/eichinger.marco?igshid=1bkdhmsrek3xt Oder einfach: eichinger.marco suchen ;) Außerdem findest Du heute auf meiner & seiner Instagrampage ein Live welches wir heute gestartet haben & welches sich aktiv um Glaubenssätze handelt. --> Unbedingt reinschauen, weil zusätzlich gibt es ein riesen Geschenk von mir & zwar das vollumfängliche "Break the Chain" Webinar indem Du lernst wie Du die Ketten Deiner Vergangenheit sprengst & das Leben Deiner Träume erschaffst! 100% Kostenlos / 100% Mehrwert / 100% Erfolg Jetzt unbedingt Downloaden *__* https://drive.google.com/drive/folders/1nHqWwy9QA4DJVZrWpIYoPaA71z9RMrrF?usp=sharing In dem Sinne lass mich gerne wissen was Dich noch für Themen interessieren & wie Du zu der Thematik Persönlichkeitsentwicklung stehst & connecte Dich doch mit mir auf Instagram & lass Dich inspirieren & schau gerne mal auf dem Dreamfactory Youtubekanal vorbei. https://www.instagram.com/lifecoachjannesgaukel/ https://www.youtube.com/channel/UCUroKXhzhIeBhNTMCVdd19Q We change the World ~Together Angefangen bei Deiner! ~ Der Wahrscheinlich Nachhaltigste Coach der Welt ~

Growing an audience, as we’ve all experienced, is a difficult task. In our increasingly digital world it can feel hard to compete with all the other companies now pushing seemingly endless quantities of content out online. If the pandemic has reaffirmed anything over the last 12 months it is that quality matters more than quantity. The businesses that thrived in the pandemic are the ones that got the content right and at the right time.  In today’s episode Twink explores content to build audiences as she’s joined by Kennedy Lynn, marketing analyst at White Marble Marketing and Vered Zimmerman, founder and managing director at FinText.  Themes identified in this podcast: Key considerations when trying to build audiences  Measuring the success of your content approach  Managing consistency across all departments How to compete and get through on social media Mistakes asset managers make when attempting to building trust with audiences Introduction to the participants: Vered Zimmerman is an experienced investment writer who helps financial services firms stand apart in a saturated, noisy market. Starting her career in maths and computer science, Vered soon realised she could use Natural Language Processing to generate better content and point to what readers find engaging. This led to the creation of FinText in 2019, a company that provides investment marketers data-backed insights on content clients want to read.  Kennedy Lynn is a recent graduate from University of Westminster in London where she obtained her master’s degree in digital business. Since then, Kennedy has concentrated on learning and improving her data analysis skills and recently joined White Marble Marketing to use her analysis skills to help maintain and grow White Marbles benchmarking service.  

Rafael Carazo Salas discusses his lab's research using multi-day multicolor high content microscopy to visualize cell fate decisions in human induced pluripotent stem cells.

Rafael Carazo Salas discusses his lab's research using multi-day multicolor high content microscopy to visualize cell fate decisions in human induced pluripotent stem cells.

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2020.07.22.215459v1?rss=1 Authors: Janssens, R., Zhang, X., Kauffmann, A., de Weck, A., Durand, E. Y. Abstract: The identification and discovery of phenotypes from high content screening (HCS) images is a challenging task. Earlier works use image analysis pipelines to extract biological features, supervised training methods or generate features with neural networks pretrained on non-cellular images. We introduce a novel fully unsupervised deep learning algorithm to cluster cellular images with similar Mode-of-Action together using only the images pixel intensity values as input. The method outperforms existing approaches on the labelled subset of the BBBC021 dataset and achieves an accuracy of 97.09% for correctly classifying the Mode-of-Action (MOA) by nearest neighbors matching. One unique aspect of the approach is that it is able to perform training on the entire unannotated dataset, to correctly cluster similar treatments beyond the annotated subset of the dataset and can be used for novel MOA discovery. Copy rights belong to original authors. Visit the link for more info

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2020.06.12.148031v1?rss=1 Authors: Antoniou, N., Prodromidou, K., Kouroupi, G., Samiotaki, M., Panayotou, G., Xilouri, M., Stefanis, L., Grailhe, R., Taoufik, E., Matsas, R. Abstract: Combining high throughput screening approaches with induced pluripotent stem cell (iPSC)-based disease models represents a promising unbiased strategy to identify therapies for neurodegenerative disorders. Here we applied high content imaging on iPSC-derived neurons from patients with familial Parkinson's disease bearing the G209A (p.A53T) lpha-synuclein mutation and launched a screening campaign on a small kinase inhibitor library. We thus identified the multi-kinase inhibitor BX795 that at a single dose effectively restores disease-associated neurodegenerative phenotypes. Proteomics profiling mapped the molecular pathways underlying the neuroprotective effects of BX795 that comprised a cohort of 118 protein-mediators of the core biological processes of RNA metabolism, protein synthesis, modification and clearance, and stress response, all linked to the mTORC1 signaling hub. In agreement, expression of human p.A53T-lpha-Synuclein in neuron-like cells affected key components of the mTORC1 pathway resulting in aberrant protein synthesis that was restored in the presence of BX795 with concurrent facilitation of autophagy. Taken together, we have developed an adaptable platform based on p.A53T iPSC-derived neurons for drug screening and identified a promising small molecule with potent neuroprotective actions as candidate therapeutic for PD and other protein conformational disorders. Copy rights belong to original authors. Visit the link for more info

Laney speaks with Abigail, @wildlingwanders, a dog trainer and owner of a high-content wolfdog named Wild. She speaks at length about what it is like living and working with a wolfdog, having raised one to work in the entertainment industry. Abigail discusses the legality of wolfdogs, the realities of owning one, and all the silly wonderful things about them as well. If you’re interested in learning more about Wild and seeing all their hiking adventures together, check out @wildlingwanders on Instagram, the merch store on Etsy at Wild’s Wares, and their Patreon for exclusive content. --- This episode is sponsored by · Anchor: The easiest way to make a podcast. https://anchor.fm/app

Iraj and Joe sit down one last time....in Humboldt County. Thats right folks, Joe co-host of the podcast is moving to Laguna Beach. 2/3 of High Content will be locate in southern California. This only means more, new, and exciting content is on the way. We are now a California based podcast. We will always love Humboldt and Iraj will be here for podcasts, events and festivals

Enrico Lugli, Humanitas Milano, Italy, speaks on "High content flow cytometry to dissect memory T cells responses". This movie is part of "Flow cytometry: from basic principles to advanced application" Course, 8-10 May 2019, ICGEB Trieste, Italy.

Iraj and Joe break up and reunite with Keaton and we talk about asap rocky's assault charges

Commercially available high-content imaging (HCI) systems, introduced in the 1990s, have provided the scientific community with a platform that offers a unique set of tools ideal for advancing high-throughput biological discoveries and therapeutic development. High-content imaging has made it possible to interrogate specific biological responses on heterotypic populations of cells, sub-populations of cells within a large population, and even within individual single cells. Today, this technology is becoming widespread across many scientific disciplines and has been used in nearly all capacities of biological and biochemical research studies. The plethora of experimental assays and cell models designed and used to produce HCI data from these sophisticated imaging systems has now grown from simple two-dimensional (2D) monolayer cell culture models to more complex three-dimensional (3D) organotypic cell models or organoids and whole organisms (examples include, Zebrafish and C. elegans) to determine biological outcomes including measuring the phenotypic characteristics in context of the tissue. Original article by O. Joseph Trask, Dr Daniel V. LaBarbera and Dr Kevin Quick If you'd like to view the original article then follow the link below: https://www.ddw-online.com/enabling-technologies/p315869-3d-cellular-imaging-advances-and-considerations-for-high-content-screening.html You can also download the original article pdf here: https://www.ddw-online.com/media/32/114685/3d-cellular-imaging-advances-and-considerations-for-high-content-screening.pdf For more information on Drug Discovery World, head to: https://www.ddw-online.com

The high content boys sit down with OEB of blo som em entertainment. We talk about xmas and shit

Saluton, estas mi Tyson Saner, your host for this early December holiday season edition of Succotash, the Comedy Soundcast Soundcast, and have I got clips for you!  (As I'm typing this I'm noticing that "have I got clips for you" feels like a question but if I were to put a question mark at the end of that sentence it feels…well, just wrong. I understand it's a rhetorical question but I never really stopped to think about what it looks like on paper or, in this case, a computer monitor…) Have I got clips for you? Of course I do — this is a clips addition of Succotash. It would be misleading and, frankly, weird if I didn't.  I've got clips from: Maltin on Movies, Christal Clear The Podcast, Master Manual, High Content Pod, Unstructured, Shenk, and Weird Adults with Little Esther. We've also got a double dose of raging moderate Will Durst's Burst O' Durst segment. Plus a reading from the website of our still-fairly-new sponsor TrumPoetry.com. Ready?  Let's go!  CLIPS  Master ManualHarmontown's "Dungeon Master to the Stars" Spencer Crittenden and game designer Cohen Edenfield discuss the finer points of leading a role-playing session in this surprisingly accessible advice show. Our clip is from the inaugural episode of Master Manual. In the episode, Spencer and Cohen answer questions and riff up a fresh new take on vampires, while the clip is from a segment called "Alternate Monster Lore". Maltin on Movies This soundcast should perhaps be entitled Maltins On Movies, as legendary film critic Leonard Maltin AND his daughter Jessie are the ultimate movie fans. They love talking about movies, especially with people who share their enthusiasm. In the epi released on November 2nd this year, their guest was comedian Dennis Miller, who is a diehard film buff. In the snippet we feature, Dennis recounts a past encounter with Leonard Maltin seasoned with references, and a discussion of The McCarthy Era of Hollywood History, as well as the beginning of a discussion about character actors. Unstructured Unstructured is a chat with people who are changing the world around them, through teaching, creating, or just living as an example. And when meeting folks, we can learn from each other, be inspired, and maybe even make a new friend, together. OUr clip is from Epi84 of the show, which dropped on October 25th of this year and featured Susan Bennett, who furnished the original voice of Siri, Apple's voice UI. She describes the recording process that when into creating Siri's voice.  ShenkComedian Sara Weinshenk interviews comedians, musicians & artists living in LA. Topics include food, fashion, cannabis & comedy. The clip we're featuring comes from the episode entitled, "Mango Juul Pods, Snail Essence & Feet Pics W/ Comedian Brandon Wardell", from this past November. Sara and Brandon yack about fashion and speculate on life as a gamer. Weird Adults with Little Esther"Little Esther" Povitsky finds out what makes her fellow comedian friends both weird and adult with an irreplaceable quirk and charm not found anywhere else. This show seems to have largely gone away but it popped back on the radar last March following a live edition performed as part of the San Francisco Sketchfest. Esther was joined by Nick Wiger of Doughboys soundcast fame where they mostly talked about food. In the clip, the host mentions she has jaw pain and that leads to a conversation about strange interactions with a few medical professionals. Christal Clear The PodcastSeeking clarity in life, to make the most of my life. "Christal Clear" is my affirmation. Our clip comes from the "Be My Guest" episode from last October, where host Christal discusses the adjustments one makes when one receives feedback on their shows and guest Jae (who has been running the Pod Dealer Network for almost a year as of this recording) explores the anxieties of being the sole host of a show. High Content Hosted by Iraj Lopez and Joe Buratti, High Content had its first live episode last month at the Savage Henry Comedy Club in Eureka, California. After local comedians Jessica Grant and Nando Molina gave interviews the hosts of HiC unexpectedly invited me up on stage for a segment. Here is a bit that I clipped off for you from the "Would You Rather" portion of the interview. And there you have it... or rather here you had it... "it" being another "Succotash The Comedy Soundcast Soundcast" clips edition... ...that is still underway now that I think about it so (ahem) here you are having it, another edition of "Succotash The Comedy Soundcast Soundcast".  Thank you for listening, thank you for retweeting us when you see our links ,and thank you for visiting www.succotashshow.com, clicking the Amazon banner right near the top of the page, and shopping as you normally would because when you do that, Amazon cuts us a small portion of that which goes to our server costs. (If you haven't done that before It's perfectly fine because we love you anyway…)  If you are listening…we also love those who don't listen as long as they are also reasonably good people…and by "We" I mean "Me". I need to clarify that because I don't wish to speak on behalf of other people when it pertains to love. It's not a healthy habit to get into…and as an expectant father I need to maintain my health in as many areas as I can.  So anyway, thank you for listening and if you like us a little you might tell your friends about us...which is one of the best ways of helping us out, and what we mean when we gently urge you to (Please) Pass the Succotash… — Tyson Saner

A recording from the very first live episode of High Content on November 13th 2018 @ The Savage Henry Comedy Club in Eureka California. @irajlopez @djbroni @highcontentpod

We are preparing for our first on location episode of High Content so that is why this episode is so short. Get excited for next week @highcontentpod @irajlopez

Lorena Zentilin, ICGEB Trieste, Italy speaks on "High content RNAi functional screening: from large libraries to functional hits". This movie has been recorded at ICGEB Trieste and is part of "RNA structure and function" Course 2018.

Saluton! It is I, Tyson Saner, your Audio-Tour-Guide or something like that.  Come with me, won't you, as we explore assorted points of interest in Soundcastland for the next hour or so. Friend-of-Succotash Hunter Block sports his new tee I've got some good ones for you.  There are clips from Launch Left, Raised By TV, Whiting Wongs, Doughboys, Wife of The Party and The Movie Court,  In addition we have a single shot of Raging Moderate Will Durst's "Burst O' Durst" (and if you're in San Francisco in the coming weeks, check out http://themarsh.org for tickets and information on his live show The Durst Case Scenario.) This installment is brought to you by Henderson's Breakaway Trousers, and I thorw in a couple of shout-outs for good measure. CLIPS LAUNCH LEFTThis show kicked off their first episode on Halloween, October 31st, of last year. According to the description on the Launch Left webpage: "Rain Joan of Arc Phoenix and Moon Unit Zappa interview extraordinary minds, mavericks, and pioneers in their unique fields. As two women from artistically uncompromising families, Rain and Moon celebrate non-conformists who achieve success by doing it their way, and support "left of center" emerging artists." That is to say...the show features established musicians promoting or "curating" less mainstream musicians.  Their guests are referred to as  "curators" and in this clip from December 26th of 2017 the curator is none other than Moon Zappa's brother Dweezil Zappa, who gives a humorous lesson in tri-tones... RAISED BY TVAmong my favorite improvisors that I hear on soundcasts such as Scott Aukerman's Comedy Bang Bang and Matt Besser's Improv4humans are Lauren Lapkus and Jon Gabrus. They have a soundcast together called Raised By TV which is described on their homesite thusly: "When Jon Gabrus and Lauren Lapkus were growing up, they watched a lot of TV. Like, a LOT of TV. Now adults with minds hopelessly warped by television, they've come together to indulge their shared obsession. On Raised By TV, Jon and Lauren revisit the best and worst TV of the late 80s and early 90s — everything from game shows and TGIF to Oprah and cereal commercials." They recently announced that Season Two of Raised By TV kicks off this April 11th. WHITING WONGSWhiting Wongs is described on iTunes as "An honest conversation about race and writing between two people who think very highly of themselves. Dan Harmon and Jessica Gao know they won't solve racism in Hollywood, but they still like to talk about it. Sometimes with guests!" By IMDB, "Jessica Gao is a writer and art director, known for Silicon Valley (2014), Rick & Morty (2013) and Lab Rats (2012). If you need credits for Harmon, perhaps you're listening to the wrong genre of soundcasts. OUr featured cli've clipped episode 4 titled "They invented those finger traps" from November 2nd, 2017 because it is the first episode  that was recorded after people had had the chance to listen and respond to the first three episodes. DOUGHBOYS "The podcast about chain restaurants," as the Doughboys listing on iTunes tells us, continuing: "Comedians Mike Mitchell and Nick Wiger review fast food/sit-down chains and generally argue about food/everything. Nick is a writer and actor, known for NTSF:SD:SUV, Matthew Perry's Testimony (Pt.2), and A Christmas Story: Only I Didn't Say Fudge. Mike is an actor and writer, known for The Birthday Boys, Love, and Michael Bolton's Big, Sexy Valentine's Day Special." Guest Bobby Lee  is credited with appearing on 162 episodes of the long-running sketch comedy show MAD TV. I deviate slightly from the norm in terms of presentation for this bit of 'soundcastery", as you'll hear it serves the content of this show better. WIFE OF THE PARTYIf you listen to comedian Bert Kreischer's soundcast Bertcast on the All Things Comedy network, you might have heard his wife LeeAnn Kreischer appear as a guest in some of the episodes. On January 31st, LeeAnn's very own soundcast dropped on iTunes and the description of Wife of the Party reads as follows: "Wife of comedian Bert Kreischer talks to friends in the mancave about, marriage, family, and what its like to be married to the life of the party." I would also like to point out the delightful "live reads" LeeAnn and Bert do for Bertcast's various sponsors.  As a fan of advertising I'm quite partial to their Blue Apron reads. (Executive Producer note: Attention, @BlueApron: We have advertising slots available on Succotash!) THE MOVIE COURT Movies finally get their day in court. Here's the online description of this unique soundcast: "Overrated classics. Unsung masterpieces. One Prosecutor. One Defense Attorney. And one judge. The Podcast that puts the movies you love or hate on trial, to decide their fate. Once and for all." That's a great description...the soundcast is as though each film was on trial to be sentenced to "movie jail" with amusing results. The Prosecutor is Stephen Krueger, the Defense is Kyle Bornheimer and Ryan (JUST Ryan) is the Judge.The clip Ive selected for you is from the 12th episode posted November 20th, 2015. (They haven't dropped an episode since January 1st of 2016 ... but I'm reluctant to send this show to the Soundcast Graveyard just yet...) EPILOG Before I leave you I'd like to give aknowledgement to a couple of soundcasters: First, a shout out to host Steve Owens of Fascination Street - a soundcast I featured in the episode of Succotash before this one.  Mr. Owens has been very vocal on Twitter about having been featured on Succotash and I appreciate it a great deal. (Cheers, Sir, and thank you for passing the Succotash!) Iraj Lopez of High Content Podcast has also given Succotash a shout out on his 29th and a 1/2 episode of his soundcast. I play it toward the end of this episode. High Content is one of several soundcasts that I listen to regularly.  This is in addition to what might be several episodes of YOUR soundcast when  I'm combing the internet looking for digital bits of corn, bacon or lima beans to incorporate into this succotash that is Succotash, the Comedy Soundcast Soundcast. If I hadn't been a consistent listener of High Content there's really no telling how long it may have been until I heard the kind words Mr. Lopez had for us. So if you do give or have given the show a mention in your soundcast, don't be shy. Contact me, Tyson Saner, on Twitter @RevT23, Executive Producer Marc Hershon on Twitter at @hershco and the show at @SuccotashShow. That way I'll be able to properly express my gratitude in as timely a fashion as I can. And that's another in the can... or the pan as it were.  If you haven't been entertained I do hope the lingering scent of bacon is some small comfort.  My thanks to you for listening. I'm grateful each and ever time you decide to spend time with us here. So I'll bid you farewell for now and gently remind you to please pass the Succotash... — Tyson Saner  

Ep 1 of HIGH CONTENT. I speak on what is to come of this podcast. This is a bad one but it only can better from here.

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2020.04.15.040394v1?rss=1 Authors: Pal, A., Kretner, B., Abo-Rady, M., Glass, H., Naumann, M., Japtok, J., Kreiter, N., Boeckers, T. M., Sterneckert, J., Hermann, A. Abstract: Intronic hexanucleotide repeat expansions (HREs) in C9ORF72 are the most frequent genetic cause of amyotrophic lateral sclerosis (ALS), a devastating, incurable motoneuron (MN) disease. The mechanism by which HREs trigger pathogenesis remains elusive. The discovery of repeat-associated non-ATG (RAN) translation of dipeptide repeat proteins (DPRs) from HREs along with reduced exonic C9ORF72 expression suggests gain of toxic functions (GOF) through DPRs versus loss of C9ORF72 functions (LOF). Through multiparametric HC live profiling in spinal MNs from induced pluripotent stem cells (iPSCs) and comparison to mutant FUS and TDP43, we show that HRE C9ORF72 caused a distinct, later spatiotemporal appearance of mainly proximal axonal organelle motility deficits concomitant to augmented DNA strand breaks (DSBs), DPRs and apoptosis. We show that both GOF and LOF were necessary to yield the overall C9ORF72 pathology. Finally, C9ORF72 LOF was sufficient, albeit to a smaller extent, to induce proximal axonal trafficking deficits and increased DSBs. Copy rights belong to original authors. Visit the link for more info