Podcasts about burgdorferi

Lyme Disease rates have doubled in the last 30 years in the USA and rates are rising across the world in affected areas. In this episode I look at Lyme, other tick-borne illnesses and the mystery symptoms they can give rise to, as well as strategies to treat and prevent them.Lyme disease factsheet:https://www.ecdc.europa.eu/en/borreliosis/facts/factsheetThe Bullseye rash: https://www.cdc.gov/lyme/signs_symptoms/rashes.htmlThe life cycle of a spirochaete: https://www.nature.com/articles/nrmicro2714B.Burgdorferi - the major cause of Lyme disease: https://www.ncbi.nlm.nih.gov/books/NBK532894/B.mayonii - another strain causing Lyme disease: https://www.cdc.gov/lyme/mayonii/index.htmlDr Horowitz Lyme questionnaire: https://caudwelllyme.com/do-you-have-lyme-disease-the-horowitz-questionnaireDifferentiating arthritis from Lyme Diseases: https://www.webmd.com/rheumatoid-arthritis/ra-or-lymeDr Horowitz interview: https://lymeconnection.org/support_and_resources/meet_the_lyme_disease_experts.html/title/dr-richard-horowitz-latest-treatments-in-lyme-disease Hosted on Acast. See acast.com/privacy for more information.

rWotD Episode 2473: Borrelia burgdorferi Welcome to random Wiki of the Day where we read the summary of a random Wikipedia page every day.The random article for Saturday, 10 February 2024 is Borrelia burgdorferi.Borrelia burgdorferi is a bacterial species of the spirochete class in the genus Borrelia, and is one of the causative agents of Lyme disease in humans. Along with a few similar genospecies, some of which also cause Lyme disease, it makes up the species complex of Borrelia burgdorferi sensu lato. The complex currently comprises 20 accepted and 3 proposed genospecies. B. burgdorferi sensu stricto exists in North America and Eurasia and until 2016 was the only known cause of Lyme disease in North America. Borrelia species are Gram-negative.This recording reflects the Wikipedia text as of 06:41 UTC on Saturday, 10 February 2024.For the full current version of the article, see Borrelia burgdorferi on Wikipedia.This podcast uses content from Wikipedia under the Creative Commons Attribution-ShareAlike License.Visit our archives at wikioftheday.com and subscribe to stay updated on new episodes.Follow us on Mastodon at @wikioftheday@masto.ai.Also check out Curmudgeon's Corner, a current events podcast.Until next time, I'm Amy Standard.

In this episode, we review the high-yield topic of⁠ ⁠⁠⁠⁠Borrelia burgdorferi⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠ ⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠from the Microbiology section. Follow ⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠Medbullets⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠ on social media: Facebook: www.facebook.com/medbullets Instagram: www.instagram.com/medbulletsofficial Twitter: www.twitter.com/medbull --- Send in a voice message: https://podcasters.spotify.com/pod/show/medbulletsstep1/message

Audiovortrag zum Thema Borellia_burgdorferi Simple und komplexe Fakten und Meinungen rund um dieses Thema aus dem Yoga Blickwinkel von Sukadev, dem Gründer des gemeinnützigen Vereines Yoga Vidya e.V. Dieser Audiovortrag ist eine Ausgabe des Naturheilkunde Podcast. Er ist ursprünglich aufgenommen als Diktat für einen Lexikonbeitrag im Yoga Wiki Bewusst Leben Lexikon. Zum ganzheitlichen Yoga kann man auch die Theorie von Karma und Reinkarnation dazu zählen. In Ayurveda Ausbildungen erfährst du mehr zum Thema Gesundheit und Prävention. Vielleicht magst du ja deine Gedanken dazu in die Kommentare schreiben. Anmerkung: Gesundheitliche Informationen in diesem Podcast sind nicht gedacht für Selbstdiagnose und Selbstbehandlung, sondern Gedankenanstöße. Bei eigener Erkrankung brauchst du einen Arzt oder Heilpraktiker. Hier findest du: Seminare mit Sukadev Seminarübersicht Yoga Vidya YouTube Live Kanal Online Seminare Video Seminare Yoga Vidya kostenlose App Yoga Vidya Newsletter Unseren Online Shop Schon ein kleiner Beitrag kann viel bewegen... Spende an Yoga Vidya e.V.!

Audiovortrag zum Thema Borellia_burgdorferi Simple und komplexe Fakten und Meinungen rund um dieses Thema aus dem Yoga Blickwinkel von Sukadev, dem Gründer des gemeinnützigen Vereines Yoga Vidya e.V. Dieser Audiovortrag ist eine Ausgabe des Naturheilkunde Podcast. Er ist ursprünglich aufgenommen als Diktat für einen Lexikonbeitrag im Yoga Wiki Bewusst Leben Lexikon. Zum ganzheitlichen Yoga kann man auch die Theorie von Karma und Reinkarnation dazu zählen. In Ayurveda Ausbildungen erfährst du mehr zum Thema Gesundheit und Prävention. Vielleicht magst du ja deine Gedanken dazu in die Kommentare schreiben. Anmerkung: Gesundheitliche Informationen in diesem Podcast sind nicht gedacht für Selbstdiagnose und Selbstbehandlung, sondern Gedankenanstöße. Bei eigener Erkrankung brauchst du einen Arzt oder Heilpraktiker. Hier findest du: Seminare mit Sukadev Seminarübersicht Yoga Vidya YouTube Live Kanal Online Seminare Video Seminare Yoga Vidya kostenlose App Yoga Vidya Newsletter Unseren Online Shop Schon ein kleiner Beitrag kann viel bewegen... Spende an Yoga Vidya e.V.!

Spirochetes - Borrelia burgdorferi

A review for the emergency physician of this common tick-borne illness. Hosts: Audrey Bree Tse, MD Brian Gilberti, MD https://media.blubrry.com/coreem/content.blubrry.com/coreem/Lyme_Disease.mp3 Download Leave a Comment Tags: Infectious Diseases Show Notes Episode Produced by Audrey Bree Tse, MD Background Most common tick-born illness in North America Endemic in Northeast, Upper Midwest, northwest California 80% to 90% in summer months Pathophysiology Ixodes tick (deer tick) has a 3-stage life cycle (larvae, nymph, adult) & takes 1 blood meal per stage Deer tick feeds on an infected wild animal (infected with spirochete Borrelia burgodrferi) then bites humans On humans, they typically move until they encounter resistance (e.g. hairline, waistband, elastic, skin fold).  It takes 24-48 hrs for B. Burgdorferi to move from the tick to the host Pathogenesis: organism induced local inflammation, cytokine release, autoimmunity No person to person transmission Clinical Presentation Stage 1: Early Symptom onset few days to a month after tick bite Erythema migrans rash: bulls eye rash seen in more than 90% of patients with Lyme disease (Irregular expanding annular lesion(s)) Regional adenopathy, intermittent fevers, headache, myalgias, arthralgia, fatigue, malaise Stage 2: disseminated/ secondary Days to weeks after tick bite Intermittent fluctuating sx that eventually resolve Triad of aseptic meningitis, cranial neuritis, and radiculoneuritis: bell palsy most common Cardiac symptoms: tachycardia, bradycardia, AV block, myopericarditis Stage 3: tertiary/ late

A review for the emergency physician of this common tick-borne illness. Hosts: Audrey Bree Tse, MD Brian Gilberti, MD https://media.blubrry.com/coreem/content.blubrry.com/coreem/Lyme_Disease.mp3 Download Leave a Comment Tags: Infectious Diseases Show Notes Episode Produced by Audrey Bree Tse, MD Background Most common tick-born illness in North America Endemic in Northeast, Upper Midwest, northwest California 80% to 90% in summer months Pathophysiology Ixodes tick (deer tick) has a 3-stage life cycle (larvae, nymph, adult) & takes 1 blood meal per stage Deer tick feeds on an infected wild animal (infected with spirochete Borrelia burgodrferi) then bites humans On humans, they typically move until they encounter resistance (e.g. hairline, waistband, elastic, skin fold).  It takes 24-48 hrs for B. Burgdorferi to move from the tick to the host Pathogenesis: organism induced local inflammation, cytokine release, autoimmunity No person to person transmission Clinical Presentation Stage 1: Early Symptom onset few days to a month after tick bite Erythema migrans rash: bulls eye rash seen in more than 90% of patients with Lyme disease (Irregular expanding annular lesion(s)) Regional adenopathy, intermittent fevers, headache, myalgias, arthralgia, fatigue, malaise Stage 2: disseminated/ secondary Days to weeks after tick bite Intermittent fluctuating sx that eventually resolve Triad of aseptic meningitis, cranial neuritis, and radiculoneuritis: bell palsy most common Cardiac symptoms: tachycardia, bradycardia, AV block, myopericarditis

Audiovortrag mit einigen Erörterungen und Darlegungen zu Borellia burgdorferi. Informationen und Anregungen zum Wort bzw. Ausdruck Borellia burgdorferi in diesem kleinen kurzen Podcast. Eine Ausgabe des Naturheilkunde Podcasts von und mit Sukadev Bretz, Yogalehrer bei Yoga Vidya. Anmerkung: Gesundheitliche Informationen in diesem Podcast sind nicht gedacht für Selbstdiagnose und Selbstbehandlung, sondern Gedankenanstöße aus dem Gebiet … „Borellia burgdorferi“ weiterlesen

Lyme disease is one of the most common infectious diseases in the United States with about 329,000 new cases each year, according to statistics released in 2015 by the CDC. Lyme disease is a potentially disabling infection caused by bacteria transmitted through the bite of an infected tick to people and pets. The efficacy and accepted regimen […] The post Lyme disease: New research on Borrelia burgdorferi persistence appeared first on Outbreak News Today.

The efficacy and accepted regimen of antibiotic treatment for Lyme disease has been a point of significant contention among physicians and patients. Newly published research finds that the Lyme bacteria, Borrelia burgdorferi survive a 28-day course of antibiotics when treated months after infection. In addition, the study also measured the antibody immune response to the bacteria both pre- and post- treatment, as this is how current diagnostics typically evaluate Lyme disease in humans. Lead author of the study, Monica Embers, PhD joined me to discuss the research and it's implications. Dr Embers is an assistant professor of microbiology and immunology at Tulane University School of Medicine and an adviser for the Bay Area Lyme Foundation.

In this episode you will learn: • Why iron is a 2 edged sword for health • Why most blood tests for iron are inaccurate • How borrelia Burgdorferi changes the way magnesium is used in your immune system Morley had been a hospital executive and consultant for 32 years until he developed a condition called “frozen shoulder.” Which opened his eyes to the power of natural medicine, and set him on his path of becoming an expert in the interplay between magnesium, copper and iron metabolism. Through his Magnesium Advocacy Group (MAG), he is committed to educating as many people as possible about the importance of iron toxicity and how magnesium and copper help regulate the well-being of all.

In this third podcast in our series on vaccination, Dr. Adam Birkenheur will discuss Borrelia burgdorferi, the bacteria that causes Lyme Disease, and also the practical approach to client communication. We’ll also explore the disease transmission and pathogenesis as well as epidemiology.

Hosts: Nels Elde and Vincent Racaniello Guests: Jessica Brown, Michael Kay, Wayne Potts, June Round, and Janis Weis From the Microbial Pathogenesis Retreat of the University of Utah School of Medicine, held at the Utah Museum of Natural History, Nels and Vincent speak with faculty members about their work on bacteria, fungi, viruses, and mirror-image biochemistry. Watch video of this episode at YouTube   Become a patron of TWiEVO This episode is sponsored by CuriosityStream, a subscription streaming service that offers over 1,400 documentaries and non­fiction series from the world’s best filmmakers. Get unlimited access starting at just $2.99 a month, and for our audience, the first two months are completely free if you sign up at curiositystream.com/microbe and use the promo code MICROBE. Science Picks Nels - An Open Letter to My Class Vincent - The Beak of the Finch by Jonathan Weiner Music on TWiEVO is performed by Trampled by Turtles Send your evolution questions and comments to twievo@microbe.tv

Psychiatrist Robert C. Bransfield, MD explains how lyme is often mistaken for psychiatric problems and how inflammation caused by b. Burgdorferi damages your nervous system. Dr. Bransfield offers himself as a resource to help your physician understand the neurological component of Lyme. Dr. Bransfield is a board certified psychiatrist who graduated from Rutgers and the George Washington School of Medicine, and finished his psychiatric residency training at Sheppard and Enoch Pratt Hospital. He first became interested in the link between infections and mental health 20 years ago when a patient referred to him by an infectious disease doctor improved tremendously after she went back on antibiotics. He has been a past president of ILADS and the NJ psychiatric association, and currently owns a private practice in Red Bank, New Jersey.

Thu, 8 Oct 2015 12:00:00 +0100 https://edoc.ub.uni-muenchen.de/18805/ https://edoc.ub.uni-muenchen.de/18805/1/Teusser_Lars-Malte.pdf Teuße

  The TWiM crew ponders the question of how a bacterium finds its middle when dividing, then divulge the transfer of interbacterial antagonism genes to eukaryotes, where they may function in innate defense.   Links for this episode:   Size independent symmetric division (Nat Commun) How does a bacterium find its middle? (Nat Struct Biol) Genes transferred from bacteria augment eukaryotic defenses (Cell) Antibacterial gene transfer (eLife) Letters read on TWiM #98 on TWiM 98 Visit microbeworld.org/twim for complete shownotes and more.

  Vincent, Elio, and Michael reveal that a soil-dwelling nematode can recognize and respond to a bacterial quorum sensing molecule through a sensory neuron.

Listen to Catherine Brissette and John Watt discuss their latest ASN NEURO paper the multifaceted responses of primary human astrocytes and brain microvascular endothelial cells to the Lyme disease spirochete, Borrelia burgdorferi.

Sat, 9 Feb 2013 12:00:00 +0100 https://edoc.ub.uni-muenchen.de/16638/ https://edoc.ub.uni-muenchen.de/16638/1/Barth_Charlotte.pdf Barth, Charlotte

Thu, 13 Dec 2012 12:00:00 +0100 https://edoc.ub.uni-muenchen.de/15282/ https://edoc.ub.uni-muenchen.de/15282/1/Bott-Fluegel_Christiane.pdf Bott-Flügel, C

BBK32, a fibronectin (Fn)-binding protein of Borrelia (B.) burgdorferi sensu lato (s.l.) which is encoded by the bbk32 gene located on the 36kb linear plasmid (lp36) of isolate B31, is playing an important role in serological diagnosis of Lyme borreliosis. Firstly, we were interested in the genomic localization of bbk32 regarding different B. burgdorferi s.l. species as well as between strains of the same species. Southern blot analyses based on 23 strains of the species B. burgdorferi sensu stricto (s.s.), B. afzelii, B. garinii and B. spielmanii revealed that position of bbk32 is rather variable between the species but also within a given species. bbk32 could be located on different linear plasmids (lp), mainly on lp23kb, lp24kb, lp25kb, lp31kb and lp36kb. The meaning of this finding remains unclear so far. Secondly, a mumber of thirteen chimeric polypeptides representing different parts of the N-terminal regions of BBK32 proteins of both B. burgdorferi s.s. isolate B31 and B. garinii isolate PHei were generated. Fn-binding capabilities of those generated polypeptides were evaluated either by Western-ligand blot-based binding assay or by enzyme-linked immunosorbent assay (ELISA)-based binding assay. Results showed that BBK32 from PHei possesses a higher Fn-binding capability than that from B31. Furthermore, the higher Fn-binding capacity is associated with four amino acids (Lysine131, Lysine145, Threonine147 and Isoleucine155) in the 32-amino acid-long segment (from position 131 to 162). Moreover, both gelatin and collagen could partially inhibit the binding of BBK32 to Fn. This suggests that BBK32 might also bind to the collagen-binding domain of Fn (repeat I6-9 and II1, 2) and partially to its N-terminal fibrin-binding domain (repeat I1-5). Though the meaning of the different Fn-binding capacities remains unclear so far, such studies may provide us with markers to define the different pathogenic potentials of various Borrelia species and strains. Thirdly, eight recombinantly prepared BBK32 homologues (either as partial or as whole) were tested in a line assay to evaluate their contribution for serologic diagnosis of Lyme borreliosis. Though BBK32 homologues could react with sera from Lyme borreliosis patients, compared with other Borrelia-antigens established in the Max von Pettenkofer Institute, these BBK32 homologues could not improve the sensitivity and specificity of the class-specific IgG or IgM antibody tests. Nevertheless, this study underlines the fact that the heterogeneity of Lyme disease Borrelia species must be taken into consideration in the microbiological diagnosis of Lyme borreliosis in European patients.

Objective: We evaluated whether immunoblotting is capable of substantiating the posttreatment clinical assessment of patients with erythema migrans ( EM), the hallmark of early Lyme borreliosis. Methods: In 50 patients, seroreactivity to different antigens of Borrelia burgdorferi sensu lato was analyzed by a recombinant immunoblot test (IB) in consecutive serum samples from a minimum follow-up period of 1 year. Antigens in the IgG test were decorin- binding protein A, internal fragment of p41 (p41i), outer surface protein C (OspC), p39, variable major protein-like sequence expressed (VlsE), p58 and p100; those in the IgM test were p41i, OspC and p39. Immune responses were correlated with clinical and treatment-related parameters. Results: Positive IB results were found in 50% before, in 57% directly after therapy and in 44% by the end of the follow-up for the IgG class, and in 36, 43 and 12% for the IgM class. In acute and convalescence phase sera, VlsE was most immunogenic on IgG testing 60 and 70%), and p41i (46 and 57%) and OspC (40 and 57%) for the IgM class. By the end of the follow-up, only the anti-p41i lgM response was significantly decreased to 24%. Conclusions: No correlation was found between IB results and treatment-related parameters. Thus, immunoblotting does not add to the clinical assessment of EM patients after treatment. Copyright (c) 2008 S. Karger AG, Basel.

Guest: Ray Stricker, MD Host: Leslie P. Lundt, MD Borrelia burgdorferi, a tickborne spirochete, is the causative agent in Lyme disease. Dr. Stricker discusses the clinical microbiology of this fascinating and often overlooked bacterium.

Thu, 6 Jul 2006 12:00:00 +0100 https://edoc.ub.uni-muenchen.de/5773/ https://edoc.ub.uni-muenchen.de/5773/1/Kaunicnik_Angelika.pdf Kaunicnik, Angelika

MICs and minimal bactericidal concentrations (MBCs) were evaluated for the four antibiotics azithromycin, amoxicillin, ceftriaxone, and doxycycline against the three main genospecies of Borrelia burgdorferi sensu lato. In MBC testing, statistically significant differences between the genospecies could be found in 7 out of 12 comparative evaluations (P < 0.05).

Die Lyme-Borreliose, ausgelöst durch den Erreger B. burgdorferi s.l., ist die häufigste von Zecken übertragene Infektionserkrankung der nördlichen Hemisphäre. Der B. burgdorferi s.l. Komplex besteht mittlerweile aus mindestens elf definierten Spezies. In Europa ist für die drei Spezies B. burgdorferi s.s., B. afzelii und B. garinii eine Humanpathogenität gesichert, für B. valaisiana wird sie zumindest vermutet. Anhand des Oberflächenproteins OspA wurden für Europa mindestens sieben verschiedene OspA-Typen definiert. Die Spezies B. burgdorferi s.s. und B. afzelii sind homogen in ihrem OspA-Typ und entsprechen Typ 1 und 2. Die Spezies B. garinii hingegen ist wesentlich heterogener und lässt sich in fünf OspA-Typen (3 bis 7) differenzieren. Die Heterogenität der Borrelien in Europa hat wichtige Implikationen für die Pathogenitätsforschung (u. a. Organotropsimus), da verschiedene Spezies bzw. OspA-Typen möglicherweise mit unterschiedlichen klinischen Manifestationsformen der Lyme-Borreliose assoziiert sind. Außerdem beeinflusst die Heterogenität maßgeblich die Entwicklung von einem europäischen Impfstoff und von diagnostischen und epidemiologischen Testsystemen. Valide Daten zur Verteilung der Spezies und OspA-Typen sind somit Vorraussetzung für derlei Entwicklungen. Jedoch ist das bisher vorhandene Datenmaterial, besonders in Bezug auf die Verteilung der OspA-Typen, sehr begrenzt, was möglicherweise auch an dem hohen Aufwand und den Kosten bisher beschriebener Differenzierungsmethoden liegt. Im Rahmen dieser Arbeit wurden einfache und zuverlässige OspA-PCR basierende Methoden entwickelt die einen sensitiven Nachweis und eine Differenzierung aller in Europa relevanten B. burgdorferi s.l. Spezies erlauben. Im Gegensatz zu vielen bisher veröffentlichten PCR Protokollen wurde die Sensitivität der Methoden mit einem umfangreichen Panel von 9 B. burgdorferi s.l. Stämmen der verschiedenen Subtypen evaluiert und die Spezifität durch Testung von 18 verwandten Spirochäten abgesichert. Nur so kann bei der Heterogenität der Borrelien die Sensitivität und Spezifität einer PCR ausreichend evaluiert werden. Die hier entwickelte RFLP Analyse erlaubt eine Differenzierung aller in Europa relevanten Spezies und zusätzlich der fünf OspA-Typen von B. garinii. Sie stellt somit ein ideales Werkzeug für notwendige epidemiologische Untersuchungen zur Heterogenität von B. burgdorferi s.l. in Europa dar. Im Weiteren ist, im Gegensatz zu vielen etablierten Typsisierungsmethoden, eine zuverlässige Differenzierung von Doppelinfektionen möglich, wie sie in Zecken und klinischem Material schon mehrfach beschrieben sind. Die entwickelte Multiplex-PCR erlaubt eine schnelle und sehr einfache Differenzierung der klinisch relevanten Spezies B. burgdorferi s.s., B. afzelii, B. garinii und B. valaisiana in einem Reaktionsansatz. Sie stellt das erste beschriebene Multiplex-PCR-Protokoll zur Differenzierung von B. burgdorferi s.l dar. Der LightCycler ist eine schnelle, moderne real-time-PCR Methode. In der vorliegenden Arbeit wurde erstmals ein LightCycler-Protokoll entwickelt, das eine Differenzierung der in Europa relevanten Spezies und mit Einschränkungen auch der verschiedenen OspA-Typen von B. garinii erlaubt. In einer Pilotstudie wurde die Verteilung von Borrelia-Spezies und OspA-Typen in Zecken und in klinischem Material untersucht. Die Borrelienpopulationen aus den Zecken der verschiedenen Sammelgebiete zeigten eine ausgeprägte Mikro- und Makroheterongenität, wobei aufgrund der Inkonstanz der Verteilungsmuster über die Zeit keine lokalen Vorhersagen über das Vorkommen einzelner Subtypen gemacht werden konnten. Ein interessanter Befund war die hohe fokale Prävalenz von OspA-Typ 4 in einem Gebiet, da diesem OspA-Typ möglicherweise eine herausragende pathogenetische Bedeutung zukommt und er bisher nur selten in Zecken gefunden wurde. Die Differenzierung von Borrelien aus klinischem Material erlaubt Rückschlüsse auf wichtige pathogenetische Zusammenhänge und Assoziationen. Bei den durchgeführten Untersuchungen konnte eine Assoziation von B. afzelii mit kutanen Manifestationen der Lyme-Borreliose bestätigt werden. Bei der Differenzierung von Isolaten von Patienten mit Neuroborreliose zeigte sich wie schon in vorangegeangenen Studien eine Dominanz der Spezies B. garinii und auf der Ebene der OspA-Typen Verteilung interessante Unterschiede in der in Bezug auf das Alter der untersuchten Patienten. Insgesamt wurde in dem untersuchten Material neben B. burgdorferi s.s., B. afzelii, B. valaisiana und allen fünf OspA-Typen von B. garinii auch die Genospezies Borrelia A14S sowie B. bissettii detektiert. Borrelia A14S stellt eine erst kürzlich beschriebene neue Genospezies von B. burgdorferi s.l. dar, deren Verteilung in Europa noch weitgehend unbekannt ist. Der Nachweis von B. bissettii aus einer Liquorprobe ist die erste Beschreibung dieser Spezies in Deutschland und wirft Fragen über ihre - bisher vermutete - Apathogenität auf. Die erhaltenen Prävalenzdaten der verschiedenen Borrelien stellen einen Schritt zur Erarbeitung einer epidemiologischen Basis für die Entwicklung eines zuverlässigen Impfstoffs und diagnostischer und epidemiologischer Testsysteme in Europa dar. Aufgrund der Unterschiede der Borrelienpopulationen in verschiedenen geographischen Regionen sind hierfür aber weitere breitgefächerte Untersuchungen auf dem ganzen Kontinent nötig. Die in dieser Arbeit entwickelten, breit evaluierten und einfachen durchzuführenden Methoden stellen für derlei Untersuchungen eine praktikable methodische Basis dar.

Tue, 4 Oct 2005 12:00:00 +0100 https://edoc.ub.uni-muenchen.de/5833/ https://edoc.ub.uni-muenchen.de/5833/1/Heimerl_Christiane.pdf Heimerl, Christiane ddc:570, ddc:500, Fakultät f

625 adult unfed I. ricinus ticks from three recreational areas located near Munich and Passau, 275 adult engorged I. ricinus ticks from dogs of Bavaria and Baden-Württemberg and 25 adult engorged I. ricinus ticks from cattle of an area in Switzerland, which is endemic for bovine granulocytic ehrlichiosis, were collected over a specific period (3/2003-3/2004). The ticks were examined for an infection with B. burgdorferi sensu lato spp., A. phagocytophilum and piroplasms by the use of Real-Time PCR and nested PCR. In addition RFLP analysis and sequencing were chosen for the differentiation of the species and OspA types of B. burgdorferi sensu lato. The examination of unfed ticks resulted in a prevalence of 35,4% for B. burgdorferi sensu lato, 4,5% for A. phagocytophilum and 1,3% for piroplasms. There was no significant difference for the infection rates of B. burgdorferi sensu lato between the different sampling areas, whereas A. phagocytophilum showed a significantly higher prevalence in one sampling side in Munich and a significantly lower prevalence in Passau. Apart from infections with only one pathogen, coinfections with B. burgdorferi sensu lato and A. phagocytophilum could be detected in 1,1% of the unfed ticks, with a local cluster in one area in Munich and 0,3% of the unfed ticks showed a coinfection with B. burgdorferi sensu lato and piroplasms. Prevalence rates of 8,4%, 4,7% and 5,1% were identified for B. burgdorferi sensu lato, A. phagocytophilum and piroplasms respectively in engorged ticks from dogs, 0,7% of these ticks were coinfected with B. burgdorferi sensu lato and A. phagocytophilum. The examination of engorged ticks from cattle revealed in a prevalence rate of 8,0% for B. burgdorferi sensu lato and 60,0% for A. phagocytophilum. The high infection rate of A. phagocytophilum probably resulted from an infection of the cattle with this pathogen. The difference in the prevalence rate of B. burgdorferi sensu lato in unfed and engorged ticks might be caused by their distinct geographical origin, the degree of blood uptake and different factors in the blood of the different host species which are able to protect the host from being infected with Borrelia. The differentiation of B. burgdorferi sensu lato into the species and OspA types showed that the clinically relevant species B. afzelii, B. burgdorferi sensu stricto and B. garinii, in which B. garinii was represented by the OspA types 3, 4, 5, 6 and 8 could be detected. Additionally, B. valaisiana, a species which is suspected of being pathogenic to humans and the recently described new Borrelia genospecies, B. spielmanii (previously A14S), were detected. Alltogether a broad heterogeneity for Borrelia species and subspecies (classified by OspA types) could be observed in unfed ticks, above all in one sampling side in Munich. Ticks engorgd from dogs and cattle showed a less heterogeneous pattern of distribution.

Ziel der vorliegenden Arbeit war es, das Vorkommen der Erreger FSMEV und Borrelia sp. in Zecken und Wildmäusen in ausgewählten Gebieten Bayerns zu bestimmen. Zu diesem Zweck wurden insgesamt 836 Wildmäuse in den Gebieten Erlangen, Grafrath und Traunstein sowie 1552 Zecken in Münchens Parkanlagen und in Schöngeising gefangen. Für die Untersuchung der Proben erfolgte die Etablierung neuer, hochsensitiver nested PCRs. Für das FSME-Virus wurde das Hüllgen-(E-) Protein als Zielgen verwendet und eine Nachweisgrenze von 0,4 TCID50 ermittelt. Zum Nachweis von Borrelien DNS diente das OspA-Gen, die Nachweisgrenze betrug 64 DNS Moleküle pro PCR Ansatz. Die Spezifität konnte anhand von 11 Stämmen gezeigt werden. Eine Inhibition der PCRs durch Probenmaterial wurden in Vorversuchen ausgeschlossen. Um die Qualität der extrahierten RNS bestätigen zu können, wurden interne Kontrollen durchgeführt, die sogenannte house-keeping Gene (Zecke: 16sRNS; Maus: Cytochrom b) als Zielsequenz hatten. Positive Proben wurden sequenziert und die ermittelten Daten für phylogenetische Analysen verwendet. In keiner der untersuchten 359 Wildmausproben und 1552 Zeckenproben konnte FSMEV nachgewiesen werden, was auf ein niedriges Infektionsrisiko mit FSMEV in den Landkreisen Erlangen, Fürstenfeldbruck, Traunstein und München hinweisen könnte. In 836 Wildmausproben konnte in 91 Proben Borrelien DNS nachgewiesen werden, womit sich eine Gesamtprävalenz von 11 % ergab. Für das Gebiet Traunstein wurde mit 34 % die höchste Borrelien-Prävalenz ermittelt. In Erlangen lag die Prävalenz in den untersuchten Proben bei 26 % und in Grafrath ergab sich eine Borrelien Prävalenz von 6 %. Dabei war B. afzelii in allen Fanggebieten die am häufigsten isolierte Spezies (81 %). B. burgdorferi wurde in 6 %, B. garinii in 2 % der untersuchten Proben isoliert. Parasitenbefall, Gewicht und Gonadengröße konnten als Einflussfaktoren für die Befallshäufigkeit mit Borrelien ermittelt werden. Ebenso konnte eine jahreszeitliche Häufung der Borrelien-Infektionen beobachtet werden, die sich jedoch in den untersuchten Regionen unterschied. Da diese Untersuchung nur eine Momentaufnahme des Erregergeschehens in den ausgewählten Gebieten wiederspiegelt, werden in Zukunft weitere Studien erforderlich sein, auch um Veränderungen in der Erregerdynamik und der Erreger-Wirts-Beziehung erfassen zu können.

During the year 2002 319 cattle from 31 farms (6 districts) of southern Bavaria were investigated for the presence of ticks during the grazing period and 287 serum samples were tested for the presence of antibodies against Borrelia burgdorferi and Babesia divergens. Ticks were detected in all 31 farms and the mean prevalence was 69 %. 3218 out of 3453 collected ticks were Ixodes ricinus. 139 nymphs, 19 larvae and 77 damaged adult specimens could only be determined to the Genus level (Ixodes). The seasonal pattern revealed the highest frequencies of ticks in May/June and September. The intensity of the infestation of the 221 positive animals was generally low. 76,5 % of parasitized individuals had 1-6 ticks/cattle and day of investigation. By contrast, individuals on pastures with appropriate tick-habitats showed up to 250 ticks/cattle and day of investigation. The percentage of infested animals in each herd varied within the period between 0 100 %. The investigation of 287 serum samples for the presence of antibodies against Borrelia burgdorferi by immunfluorescence techniques (IFAT), revealed antibody-titers ≥ 1:64 for 45,6 % of the animals. 27 of 31 farms showed prevalences of Borrelia burgdorferi ranging from 20 to 100 %. There were no significant differences of positive cattle between the various areas. However, a significant correlation could be detected between the number of ticks per cattle and the antibody concentration of anti-Borrelia IgG. By contrast, there was no significant correlation between the age of the animals and the serum antibody-titers. For comparative reasons, 64 IFAT-positive serum samples were tested by Westernblot techniques for the presence of antibodies cross-reacting with Borrelia garinii antigens. These analyses revealed that 69 % of the samples reacted positively, 28 % were unclear and 3 % were negative. The investigation of the 287 cattle sera for Babesia divergens by means of IFAT revealed one positive animal with an antibody-titer of 1:16. Finally, in an additional enzyme linked immunosorbent assay (ELISA) 105 serum samples from 18 farms and 13 pooled sera including 45 cattle from the other 13 farms were tested for the presence of antibodies against Fasciola hepatica. In 96,8 % of the farms antibodies against Fasciola hepatica could be detected.

Wed, 1 Jan 1986 12:00:00 +0100 https://epub.ub.uni-muenchen.de/8806/1/reinfection_with_borrelia_burgdorferi_8806.pdf Borasio, Gian Domenico; Einhäupl, Karl Max; Preac-Mursic, Vera; Wilske, Bettina; Neubert, Uwe; Pfister, Hans-Walter ddc:610, Medizin