Podcasts about retroviral

Mike Sharman has helped more brands go viral than any other agency in Africa. And in this episode of Good Things with Brent Lindeque, we dive deep into the mind behind the magic. From his early days of hustle to building Retroviral into a creative powerhouse, Mike shares what it really takes to make content that connects, campaigns that count and why he's chosen to build his business right here in South Africa. We talk creativity, chaos, doing business with heart and why betting on Mzansi is always worth it. It's insightful, inspiring and properly entertaining.

If you want your brand to go viral, I guess Mike Sharman is your guy! My guest is the founder and Chief Creative Officer for Retroviral. I've seen some amazing work from that business and they are what the industry needs, BRAVERY! Mike and I spoke about exactly that, what the marketing world needs... DISRUPTION, the award winning work they have managed to do for brands that has undeniably made them famous. Lover of PR and the power it has, challenger of AI in the marketing world and the bravery of Mike and his team inspire us to be better, do better, and challenge AI and all tech, but use it to start the conversation through show and sell!

In this episode, we dive into the incredible power of sport to change lives and unite communities. Join us as we chat with Mike Sharman, founder of Retroviral and a passionate advocate for positive change in South Africa. Mike discusses the urgent need to support our athletes, especially those competing in the Olympics and Paralympics, and sheds light on the disparities faced by lesser-known "Cinderella Sports." Mike passionately calls for more investment in the right areas, ensuring that athletes, rather than officials, receive the recognition and resources they deserve. He emphasises the importance of backing our sportsmen and women, not just financially but through our encouragement and presence. Tune in for an inspiring conversation about how we can rally behind our athletes and help them achieve their dreams, while also fostering a stronger sense of community and national pride. Let's unite for our athletes and ensure that no sport, and no athlete, is left behind.

Locked up anti-retroviral drugs and already existing ones not sufficient for AIDS patients in Ghana - Ernest Amoabeng Ortsin

This is a conversation with Retroviral founder Mike Sharman. Retroviral has made more brands "go viral" globally than any other agency in Africa. Focusing on strategy, content creation, dissemination and reporting . Learn more here https://retroviral.co.za/ To learn more about our new partnership with Sage and how #SageForSmallBiz can impact your business here: https://www.sage.com/en-za/size/small-business/ Signup for a free trial using the offer code SageMash The Mashstartup podcast explores practical learnings and candid ​journeys of Africa's emerging entrepreneurs and creators. The Mashstartup Podcast profiles the most talented creators, impactful entrepreneurs & high performing professionals with the purpose to drive insights and tactics to help you build what you believe in. Youth entrepreneurship evangelist Mashudu Modau is an influential voice in the small business community. As founder of Lutcha and Founders Sauce – he connects people and builds platforms to help African entrepreneurs grow. Learn more www.mashstartsup.co.za Visit

Tim Fenton, Ph.D., University of Southampton, shares his work on the roles of APOBEC3 genes. Hear how he is investigating APOBEC regulation and function in keratinocytes. Series: "Stem Cell Channel" [Health and Medicine] [Show ID: 38724]

Tim Fenton, Ph.D., University of Southampton, shares his work on the roles of APOBEC3 genes. Hear how he is investigating APOBEC regulation and function in keratinocytes. Series: "Stem Cell Channel" [Health and Medicine] [Show ID: 38724]

Tim Fenton, Ph.D., University of Southampton, shares his work on the roles of APOBEC3 genes. Hear how he is investigating APOBEC regulation and function in keratinocytes. Series: "Stem Cell Channel" [Health and Medicine] [Show ID: 38724]

Tim Fenton, Ph.D., University of Southampton, shares his work on the roles of APOBEC3 genes. Hear how he is investigating APOBEC regulation and function in keratinocytes. Series: "Stem Cell Channel" [Health and Medicine] [Show ID: 38724]

Tim Fenton, Ph.D., University of Southampton, shares his work on the roles of APOBEC3 genes. Hear how he is investigating APOBEC regulation and function in keratinocytes. Series: "Stem Cell Channel" [Health and Medicine] [Show ID: 38724]

Tim Fenton, Ph.D., University of Southampton, shares his work on the roles of APOBEC3 genes. Hear how he is investigating APOBEC regulation and function in keratinocytes. Series: "Stem Cell Channel" [Health and Medicine] [Show ID: 38724]

Tim Fenton, Ph.D., University of Southampton, shares his work on the roles of APOBEC3 genes. Hear how he is investigating APOBEC regulation and function in keratinocytes. Series: "Stem Cell Channel" [Health and Medicine] [Show ID: 38724]

South African | Co-founder of Retroviral & Matchkit | Linkedin  Social Media : Linkedin | Instagram | Twitter  You can welcome to support my podcasts on Patreon. Today's guest is Mike Sharman, the co-founder & Chief Creative Officer of Retroviral but really I should introduce him as a serial entrepreneur. Mike is one of those guys who understands media, communications and strategy, while talking to him, I saw why he is successful. He is full of energy and passion for what he does. By the way, I hope you love what you do.  Communications agencies like the ones founded by Mike in South Africa or Joan in Rwanda (episode 62) are changing the media landscape for Africans. I can say that it was about time. In Burundi, let me give a shout out to the @Burundionthemap, that is showcasing what many need to see & hear about my country. Parole podcast is on all the major podcast platforms - Spotify, Apple, Google, Afripods, Audapp if you are in Nigeria etc. 1M downloads per episode - so here's me asking for your help - share, share, share.  Parole podcast via Boyi Studios is also on Patreon, if you want to support my podcasts, find the link on the show notes. Until next time!

Peter Armitage, CEO at Anchor Capital looks at how protests in China over the country's increasing zero-Covid policy are affecting global markets.       Wandile Sihlobo, senior fellow at the department of agricultural economics at Stellenbosch University analyses five myths about land reform in South Africa.     Then on Other People's Money, Mike Sharman, the founder of Retroviral spoke about his relationship with his own finances. See omnystudio.com/listener for privacy information.

Guest: Mike Sharman | Co-Founder at RetroviralSee omnystudio.com/listener for privacy information.

Mike Sharman considers himself to be a creative and an entrepreneur. He used both of these traits to build a successful $1m social media agency called Retroviral, which is responsible for making more brands go viral than any other agency in Africa.After successfully launching and then scaling Retroviral, Mike sold a majority stake in the business to a large corporate. That's when the gaps in his financial knowledge started to affect his role as CEO, the stability of the acquisition and put the day to day running of the business into a precarious position.In this episode, Mike and I talk about his financial shortcomings, how he muddled through the acquisition and the impressive turnaround that followed.Buy Mike's new book - http://brandalismbook.com/Work with Retroviral - https://retroviral.co.za/Follow Mike on Twitter - @mikesharman

Stage 2 loadhsedding rears its head just before winter, as we've just swallowed another 9% tariff hike from Eskom, with the UN warning of runaway global food prices, unseen since 2011, and with the pandemic still lurking in the background, its fair to say that all of us have had enough. Inflation is creeping up beyond the reserve bank's target range of 6% according to most economists. And yet that is still below global inflation rates which are closer to7 and 8% in the US and Europe. Inflation will impact the spending choices of households, but it will also impact brands, who might have to decide which programs and initiatives to pause or cancel. This rise in the cost of living and the subsequent reduction in disposable income impacts margins. So how do brands navigate this tricky terrain? Let's welcome our panel to find out Dashni Vilikazi, MD of The Mediashop; Thulani Sibeko, Chief Marketing Officer of Standard bank; Jeremy Sampson, MD of Brand Finance Africa & Mike Sharman, founder of digital agency Retroviral

In Brandalism, the follow-up to his bestselling, award-winning debut book The Best Dick, Mike Sharman delves into the (start)ups and downs associated with brand building and the need for business to dismantle and vandalise its perceived, public-facing, persona.The future of PR and influence, when - or if - to launch a new business as opposed to a division, raising capital, the impact of presentations, start-up school fees, and emphasis on a manifesto rather than the purpose, are the aspects Mike obsesses over in this insightful read, wrapped in his trademark, comedic, copy.Mike Sharman, the co-founder of the creative, digital agency Retroviral that has made more brands 'go viral', globally than any other agency in Africa, uses his unique storytelling proposition to provide insight into 12 years of building a business from scratch while elevating his clients to emotional, (commercial) cult status.

Sometimes you have to brandalize to revitalize. Mike Sharman has been leading change in the marketing world by helping brands achieve virality for the past 13 years. Hear how the most recent documentaries on #Netflix, #tinderswindler and #myoctopusteacher, presented the perfect opportunity to lead two staple and traditionally successful brand leaders into the edgey bold new frontiers of parody, virality and increased sales - and all of this amidst the noisy over saturated advertising and content marketplace. Of course you also won't want to miss our new engaging podcast format - and Mike's Aussie and New Zealand accent as he slips easily into character of his former boss. If you are looking for an inspiring (often humorous) change leadership story, mixed with some great tips, tricks and insight, then catch our latest podcast release with the passionate and entertaining CEO and co-founder of #Retroviral, Mike Sharman - change cultivator extraordinaire.

Sometimes you have to brandalize to revitalize. Mike Sharman has been leading change in the marketing world by helping brands achieve virality for the past 13 years. Hear how the most recent documentaries on #Netflix, #tinderswindler and #myoctopusteacher, presented the perfect opportunity to lead two staple and traditionally successful brand leaders into the edgey bold new frontiers of parody, virality and increased sales - and all of this amidst the noisy over saturated advertising and content marketplace. Of course you also won't want to miss our new engaging podcast format - and Mike's Aussie and New Zealand accent as he slips easily into character of his former boss. If you are looking for an inspiring (often humorous) change leadership story, mixed with some great tips, tricks and insight, then catch our latest podcast release with the passionate and entertaining CEO and co-founder of #Retroviral, Mike Sharman - change cultivator extraordinaire.

Sometimes you have to brandalize to revitalize. Mike Sharman has been leading change in the marketing world by helping brands achieve virality for the past 13 years. Hear how the most recent documentaries on #Netflix, #tinderswindler and #myoctopusteacher, presented the perfect opportunity to lead two staple and traditionally successful brand leaders into the edgey bold new frontiers of parody, virality and increased sales - and all of this amidst the noisy over saturated advertising and content marketplace. Of course you also won't want to miss our new engaging podcast format - and Mike's Aussie and New Zealand accent as he slips easily into character of his former boss. If you are looking for an inspiring (often humorous) change leadership story, mixed with some great tips, tricks and insight, then catch our latest podcast release with the passionate and entertaining CEO and co-founder of #Retroviral, Mike Sharman - change cultivator extraordinaire.

See omnystudio.com/listener for privacy information.

I am thrilled to tell you that we're partnering with the Glenlivet to bring you a series of conversations with innovative entrepreneurs over the next 10 episodes. The whiskey connoisseurs amongst you will know the Glenlivet as an award winning sought after collection of Speyside single malt Scotch whiskies, just like that collection, this series of conversations, we'll have a little something for everyone. We're going to be talking to inventors creatives, financial wunderkinds, problem solvers and more, celebrating those who strive to find answers and solve problems, where others have given up even asking questions. In our first episode, I talked to arguably South Africa's leading expert on branded content, an old friend and colleague in the digital marketing industry, Mike Sharman. Mike is the CEO of marketing agencies Retroviral and Retroactive and more recently a co-founder with ex-Springbok rugby player Bryan Habana and Ben Karpinski of a company called Match Kit, an exciting tech startup that was recently included in a prestigious alpha code incubator program. I talked to Mike about this, and other remarkable achievements. About his insatiable and infectious enthusiasm for creative work, and about the lessons he learned building these incredible businesses. Before we get into the show, please remember to pay close attention and keep an eye on my social media. Toward the end of the series, we'll be giving away some incredible Glenlivet hampers to a few lucky listeners. Glenlivet Website · Connect with Mike Sharman on LinkedIn · Retroviral · Match Kit · MikeStopforth.com · Connect with Mike on LinkedIn · Follow Mike on Twitter · 48h crisis communication consultancy

I am thrilled to tell you that we're partnering with the Glenlivet to bring you a series of conversations with innovative entrepreneurs over the next 10 episodes. The whiskey connoisseurs amongst you will know the Glenlivet as an award winning sought after collection of Speyside single malt Scotch whiskies, just like that collection, this series of conversations, we'll have a little something for everyone. We're going to be talking to inventors creatives, financial wunderkinds, problem solvers and more, celebrating those who strive to find answers and solve problems, where others have given up even asking questions. In our first episode, I talked to arguably South Africa's leading expert on branded content, an old friend and colleague in the digital marketing industry, Mike Sharman. Mike is the CEO of marketing agencies Retroviral and Retroactive and more recently a co-founder with ex-Springbok rugby player Bryan Habana and Ben Karpinski of a company called Match Kit, an exciting tech startup that was recently included in a prestigious alpha code incubator program. I talked to Mike about this, and other remarkable achievements. About his insatiable and infectious enthusiasm for creative work, and about the lessons he learned building these incredible businesses. Before we get into the show, please remember to pay close attention and keep an eye on my social media. Toward the end of the series, we'll be giving away some incredible Glenlivet hampers to a few lucky listeners. Glenlivet Website · Connect with Mike Sharman on LinkedIn · Retroviral · Match Kit

The Team with Invincible Values discusses whether dogs or cats passively carry SARS-CoV-2 on hair and foot pads, maternal to fetal transfer of antibodies in infected or vaccinated pregnant women, and evidence that retroviruses infected cetaceans before and after they moved into the oceans. Hosts: Vincent Racaniello, Dickson Despommier, Alan Dove, and Brianne Barker Subscribe (free): iTunes, Google Podcasts, RSS, email Become a patron of TWiV! Links for this episode SARS-CoV-2 on dogs and cats (Viruses) Maternal to neonatal transfer of SARS-CoV-2 antibodies (J Clin Investîmes) Retroviral evolution in cetaceans and their ancestors (Plos Path( Letters read on TWiV 781 Timestamps by Jolene. Thanks! Weekly Picks Dickson – Current Map | U.S. Drought Monitor Brianne – A comic strip about immune responses Alan – Superliminal game Vincent – Birds are dying in NJ Listener Picks 2listening1 – Renewal Em – Cognitive Surplus Intro music is by Ronald Jenkees Send your virology questions and comments to twiv@microbe.tv

The Team with Invincible Values discusses whether dogs or cats passively carry SARS-CoV-2 on hair and foot pads, maternal to fetal transfer of antibodies in infected or vaccinated pregnant women, and evidence that retroviruses infected cetaceans before and after they moved into the oceans. Hosts: Vincent Racaniello, Dickson Despommier, Alan Dove, and Brianne Barker Subscribe (free): iTunes, Google Podcasts, RSS, email Become a patron of TWiV! Links for this episode SARS-CoV-2 on dogs and cats (Viruses) Maternal to neonatal transfer of SARS-CoV-2 antibodies (J Clin Investîmes) Retroviral evolution in cetaceans and their ancestors (Plos Path( Letters read on TWiV 781 Timestamps by Jolene. Thanks! Weekly Picks Dickson – Current Map | U.S. Drought Monitor Brianne – A comic strip about immune responses Alan – Superliminal game Vincent – Birds are dying in NJ Listener Picks 2listening1 – Renewal Em – Cognitive Surplus Intro music is by Ronald Jenkees Send your virology questions and comments to twiv@microbe.tv

Guest: Mike Sharman | Co-Founder at Retroviral | See omnystudio.com/listener for privacy information.

In this episode we're in conversation with Greg Bailie, Head of TikTok Brand x Partnership division at Webfluential, and Mike Sharman, Founder of Retroviral and a host of other companies. Grab a cup of coffee and listen as we discuss: • Why TikTok is popular and why it became even more so during 2020 • Including TikTok in influencer campaigns • Is it really just for the youth? • Why TikTok could be a good fit for certain brand campaigns • How TikTok campaigns are conceptualised, implemented and measured Listen to the episode here: https://band.link/coffeeconversations Please remember to subscribe to the podcast and share. Connect with Greg on LinkedIn here: https://www.linkedin.com/in/greg-bailie/ Connect with Mike on LinkedIn here: https://www.linkedin.com/in/mikesharman/ Coffee Conversations about Influencer Marketing podcast is available wherever you find your favourite podcasts. Subscribe to Nfluential's newsletter to stay updated here https://bit.ly/2DxoDC9 and follow us on Twitter at https://twitter.com/NfluentialSA and LinkedIn https://www.linkedin.com /company/nfluentialsa. Nfluential Influencer Marketing Strategists

In this episode we’re in conversation with Greg Bailie, Head of TikTok Brand x Partnership division at Webfluential, and Mike Sharman, Founder of Retroviral and a host of other companies. Grab a cup of coffee and listen as we discuss: • Why TikTok is popular and why it became even more so during 2020 • Including TikTok in influencer campaigns • Is it really just for the youth? • Why TikTok could be a good fit for certain brand campaigns • How TikTok campaigns are conceptualised, implemented and measured Listen to the episode here: https://band.link/coffeeconversations Please remember to subscribe to the podcast and share. Connect with Greg on LinkedIn here: https://www.linkedin.com/in/greg-bailie/ Connect with Mike on LinkedIn here: https://www.linkedin.com/in/mikesharman/ Coffee Conversations about Influencer Marketing podcast is available wherever you find your favourite podcasts. Subscribe to Nfluential’s newsletter to stay updated here https://bit.ly/2DxoDC9 and follow us on Twitter at https://twitter.com/NfluentialSA and LinkedIn https://www.linkedin.com /company/nfluentialsa.

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2020.09.02.279273v1?rss=1 Authors: Chen, G., Li, W., Xiang, Z., Xu, L., Liu, M., Wang, Q., Lei, W. Abstract: Regenerating functional new neurons in the adult mammalian central nervous system (CNS) has been proven to be very challenging due to the inability of neurons to divide and repopulate themselves after neuronal loss. In contrast, glial cells in the CNS can divide and repopulate themselves under injury or disease conditions. Therefore, many groups around the world have been able to utilize internal glial cells to directly convert them into neurons for neural repair. We have previously demonstrated that ectopic expression of NeuroD1 in dividing glial cells can directly convert reactive glial cells into neurons. However, Wang et al. recently posted an article in bioRxiv challenging the entire field of in vivo glia-to-neuron conversion after using one single highly toxic dose of AAV (2x1013 gc/ml, 1 ul) in the mouse cortex, producing artifacts that are very difficult to interpret. We present data here that reducing AAV dosage to safe level will avoid artifacts caused by toxic dosage. We also demonstrate with Aldh1l1-CreERT2 and Ai14 reporter mice that lineage-traced astrocytes can be successfully converted into NeuN+ neurons after infected by AAV5 GFAP::NeuroD1. Retroviral expression of NeuroD1 further confirms our previous findings that dividing glial cells can be converted into neurons. Together, the incidence of Wang et al. sends an alarming signal to the entire in vivo reprogramming field that the dosage of viral vectors is a critical factor to consider when designing proper experiments. For AAV, we recommend a relatively safe dose of 1x1010 - 1x1012 gc/ml (~1 ul) in the rodent brain for cell conversion experiments addressing basic science questions. For therapeutic purpose under injury or diseased conditions, AAV dosage needs to be adjusted through a series of dose finding experiments. Moreover, we recommend that the AAV results are further verified with retroviruses that mainly express transgenes in dividing glial cells in order to draw solid conclusions. Copy rights belong to original authors. Visit the link for more info

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2020.06.18.160077v1?rss=1 Authors: Wairkar, Y. P., Tang, S.-J., Bush, K., Barber, K., Martinez, J. Abstract: The success of antiretroviral therapy (ART) has improved the survival of HIV-infected patients significantly. However, significant numbers of patients on ART whose HIV disease is well controlled show peripheral sensory neuropathy (PSN), suggesting that ART may cause PSN. Although the nucleoside reverse transcriptase inhibitors (NRTIs), one of the vital components of ART, are thought to contribute to PSN, the mechanisms underlying the PSN induced by NRTIs are unclear. In this study, we developed a Drosophila model of NRTI-induced PSN that recapitulates the salient features observed in patients undergoing ART: PSN and nociceptive hypersensitivity. Furthermore, our data demonstrate that pathways known to suppress PSN induced by chemotherapeutic drugs are ineffective in suppressing the PSN or nociception induced by NRTIs. Instead, we found that increased dynamics of a peripheral sensory neuron may underlie NRTI-induced PSN and nociception. Our model provides a solid platform in which to investigate further mechanisms of ART-induced PSN and nociceptive hypersensitivity. Copy rights belong to original authors. Visit the link for more info

TechCentral — Mike Sharman, founder of digital marketing agency Retroviral, joins TechCentral editor Duncan McLeod for a chat about MatchKit.io, a new website builder aimed at sports professionals. Founded by Sharman, Springbok rugby player Bryan Habana, and sports content creator and broadcaster Ben Karpinski, Retroactive - which owns MatchKit.co - intends to help professional sportsmen and sportswomen worldwide develop a digital presence for themselves. In the podcast, Sharman explains that while most sports people have a presence on social media, they often don't have their own website. The idea behind the website is to make it easier for sports professionals to develop an online presence, raise money for their chosen charities and make money from selling merchandise. In the podcast, Sharman explains how the business model works, how he and Habana came up with the idea for the platform, and where they see it going next. Sharman also looks back at 10 years of Retroviral and some of the big campaigns the agency has worked on in that time, including with Nando's and RocoMamas. Don't miss the discussion!

Hot of the Press: Lopinavir–Ritonavir NOT Effective in Severe COVID19 NEJM March 18, 2020 Dr RR Baliga's 'Got Knowledge Doc' Podcasts for Physicians Not Medical Advice or Opinion

Binwe Adebayo is a writer, strategist, and academic who is currently using cyber-ethnography to do her masters degree on Black Twitter. Yeah, I had no idea what cyber-ethnography was either, but you'll find out just like I did on the podcast. Binwe is goal orientated and because of that has achieved quite a lot in her 25 years on this earth. She's already racked up valuable experience across multiple disciplines, from being a journalist for City Press to finding a passion for being a strategist at Retroviral. She now holds the title of Senior Associate and Strategist at Nicola Cooper and Associates whilst also working on her masters. We get into giving up drinking to focus on her studies, the complexities of Black Twitter, the effects of social media and advertising on our lives, and we look at the positives and negatives of influencer culture. It's an eye-opening chat about things that affect us all daily, enjoy. https://almostperfect.co.za/ep-26-binwe-adebayo/

Mike Sharman shares how business owners can leverage the power of social media to build valuable relationships with their customers. Mike is the man behind Retroviral, an award-winning digital communications agency who have worked with the likes of Nando's and RocoMamas.

JacPod — Rob Greenstein has just opened Africa’s first LEGO store in Sandton City. Dean caught up with him and Retroviral’s Mike Sharman to discuss one of the most loved brands in the world and the pressures of launching it in South Africa.

December 1st is World AIDS Day. HIV AIDS affects 35 million people worldwide, and although the number of new infections is slowly decreasing, last year it still caused one million deaths. The virus, HIV, attacks the body's immune system by infecting white blood cells, also called "lymphocytes". Lewis Thomson has been finding out what the future holds for treatment, and what it's like to be diagnosed with the virus, and met with Professor Andrew Lever from the University of Cambridge, who researches the virus. Like this podcast? Please help us by supporting the Naked Scientists

December 1st is World AIDS Day. HIV AIDS affects 35 million people worldwide, and although the number of new infections is slowly decreasing, last year it still caused one million deaths. The virus, HIV, attacks the body's immune system by infecting white blood cells, also called "lymphocytes". Lewis Thomson has been finding out what the future holds for treatment, and what it's like to be diagnosed with the virus, and met with Professor Andrew Lever from the University of Cambridge, who researches the virus. Like this podcast? Please help us by supporting the Naked Scientists

Dr Noriyuki Kasahara speaks with ecancertv at AACR2016 about his cancer-targeting retroviral therapies which illicit host immune responses. Historically, oncolytic viruses have struggled to compete with host immunity to reach their target and the immunosuppresive tumour environment. But by using a retroviral factor, Dr Kasahara's lab reports selective targeting of cancer cells with a gene primer that in turn responds to a non-toxic pro-drug to kill the 'infected' tumour cells. Dr Kasahara reports significant extension to patient survival compared to current chemotherapy treatments, with no systemic toxicity and high-tumour specificity. Trials are ongoing.

Storytelling. It’s one of the biggest buzzwords in business—storytelling—and it’s how savvy companies are satisfying the public’s never-ending hunger for content. With compelling characters, relatable plots and, most important, authenticity, innovative story tellers are connecting with consumers, colleagues and investors on an emotional level – something which will always distinguish your business from that of a competitors. I chat to Mike Sharman about how entrepreneurs can craft a great story for the businesses and its products and services.

Os Heróis da Heròs Sans Frontiere investigam uma droga provocadora de Limiares no mundo Pós-Evento, usando seus superpoderes e habilides! Olá! Nessa quarta Mesa Predestinada apresentamos o jogo que tivemos em um cenário em Playtest, Wearing the Cape, um cenário de Supers onde após o misterioso Evento, pessoas começaram a desenvolver superpoderes. Essas pessoas, chamadas de breakthroughs, ou Liminares, desenvolvem seus poderes após eventos traumáticos (de maneira positiva ou negativa) ou que os coloquem em risco de vida, e seus poderes são relacionados com os eventos sofridos. Nessa Mesa, os conhecidos membros dos podcasts da Família Fate (Fate Masters, Rolando +4 e Mesas Predestinadas) Rafael Meyer (o Velho Lich do Fate Masters) e Jean da Silva (do Rolando +4) enfrentam o desafio de descobrir um misterioso Senhor Tribal que, por meio de sua tecnologia superheroica, procura criar sua própria armada de supersoldados! Na maior instalação para refugiados do mundo pós-Evento, a Roque Santeiro, eles devem auxiliar a manter a ordem como parte dos Heròs Sans Frontiere (Heróis Sem Fronteira), uma organização superheroica ligada aos Médicos Sem Fronteiras. Serão eles capazes de enfrentar os perigos que a Serra Leoa pós-Evento os oferece? E como que uma droga pode despertar poderes em pessoas normais? Esse é o desafio proposto aos heróis! Nosso contato para esse podcast é o email mesaspredestinadas@gmail.com, entre outros contatos a serem estipulados. Link para o programa em MP3 Participantes: Fábio Emilio Costa (Narrador) Jean da Silva (Chimera) Rafael Sant’anna Meyer (Blackstar) Duração: 187min Cronologia do Podcast: 00:00:10 - Apresentação básica do Cenário e dos Personagens 00:05:44 - Blackstar e Chimera chegam a Serra Leoa e ao Campo de Refugiados Roque Santeiro 00:11:41 - Os heróis chegam a Roque Santeiro e são apresentados à equipe já estabelecida da Heròs Sans Frontiere 00:21:04 - Os heróis são escalado para sua primeira missão na Roque Santeiro 00:30:00 - Enquanto os refugiados e suprimentos são embarcados nos veículos de transportes, Blackstar tem um mal pressentimento 00:36:50 - Blackstar percebe que seu mal pressentimento tem motivo - o combo é atacado! 01:16:16 - Blackstar e Chimera suspeitam que foram testados e que quem criou Chimera pode estar envolvido 01:18:45 - Diana explica a situação e agora os personagens vão investigar sobre o Cientista Maluco local 01:24:37 - Blackstar e Chimera começam a investigar sobre a Droga que provoca Limiares 01:30:21 - Blackstar e Chimera consultam alguns líderes tribais locais por pistas relativas a Derek Kwazani 01:38:45 - Blackstar usa seus contatos na cidade, e um Senhor de Armas conhecido dela oferece pistas sobre um Witch Doctor local vendedor da Droga do Limiar 01:42:45 - O Jean precisou sair, então Chimera é chamado de volta à Roque Santeiro enquanto Blackstar questiona o Witch Doctor 01:46:48 - Um dos Quatro de Elite de Kwazani confronta Blackstar, enlouquecendo ao citar “pai e filho” 01:51:35 - Panther e Blackstar começam a negociar por causa da Filha de Panther 02:00:02 - Blackstar conseguer um novo Retroviral para a Filha de Panther 02:02:08 - Blackstar retorna à Roque Santeiro e obtem informações adicionais sobre a Elite de Derek Kwazani 02:03:55 - Blackstar procura a família de Kilimanjaro por informações, e acaba encontrando a mesma 02:17:35 - Blackstar repassa as informações obtidas com Kilimanjaro para Diana, e começa a preparar-se o ataque 02:22:07 - A invasão começa, com um primeiro combate contra seguranças da base de Kwazani 02:29:01 - No segundo nível, os heróis são obrigados a se render, sob risco da morte de Jiminy Cricket (que apesar de herói, tem o tamanho de uma criança de nove anos!) 02:35:02 - O momento no qual Derek Kwazani revela seu plano para “devolver a África a seu lugar de respeito” 02:39:40 - Os heróis são colocados em uma Cúpula do Trovão contra alguns Limiares ensandecidos 02:55:32 - Os heróis escapam e Derek Kwazani se vê em uma situação em que tem que negociar sua rendição 03:04:55 - A Conclusão da Aventura… Links Relacionados: Sobre a série de livros Wearing the Cape Sobre o Playtest de Wearing the Cape: The Roleplaying Game Comunidade do Google+ para o Playtest de Wearing the Cape: The Roleplaying Game Ficha do Quimera Ficha da Blackstar Ficha da Sugarplum (Nome Verdadeiro: Eileen McRae) (em Inglês) Ficha do Jiminy Cricket (Nome Verdadeiro: Joshua McCarthy) (em Inglês) A Aventura mestrada (em Inglês) Link para mesa gravada no YouTube Link para a comunidade do Google+ do Mesas Predestinadas Comente esse post no site do Mesas Predestinadas! Assine no iTunes Trilha Sonora do Podcast: Victory of Heroes por Shane Ivers Ascension por Shane Ivers Conquer por Shane Ivers Passage of Arms por Shane Ivers Voyage of Discovery por Shane Ivers

Mike Sharman is the co-founder of RetroViral, an award winning digital communications agency. Winning fancy dresser and creator of words like “radvertising” and “adtrepreneur” In this episode you will learn: Marketing lessons from doing stand up comedy The most important thing to get ahead in your field How following a sound business model actually allows you more freedom How to get people to buy into what your saying Why being analytical can sometimes be more creative The one thing that will separate you from a crowd Links mentioned in this episode: Justine Musks article on being obsessed  Mike Sharman's Creative Morning's Talk   Get in touch with Mike Sharman Retroviral Twitter @Retroviral Twitter @MikeSharman Mike's Personal Blog- The Sharmanator Thanks for listening and a huge thank you to Mike for joining me on this episode! If you haven't already, subscribe to the show on iTunes to be notified each time a new episode comes out. Keep in touch with us via social media, Instagram and Twitter are probably the best to stay in the loop. Also, please leave an honest review for The Creative Slice Podcast on iTunes. Ratings and reviews are extremely helpful and greatly appreciated! They do matter in the rankings of the show, and I read each and every one of them. Would also love to hear any suggestions for future guests that you recommend I have on the show.   Looking for something else to listen to? Listeners of The Creative Slice podcast can grab a FREE Audiobook from Audible! (click the affiliate link below) I'd recommend Chris Guillebeau's “Happiness of Pursuit” or Todd Henry's “Louder than Words” but it's completely up to you what you pick!

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Video Podcast (CC)Aired date: 4/11/2012 3:00:00 PM Eastern Time

Audio PodcastAired date: 4/11/2012 3:00:00 PM Eastern Time

Thu, 15 Dec 2011 12:00:00 +0100 https://edoc.ub.uni-muenchen.de/14396/ https://edoc.ub.uni-muenchen.de/14396/1/Nagel_Jens.pdf Nagel, Jens ddc:570, ddc:500, Fakultät für Biol

Fri, 13 May 2011 12:00:00 +0100 https://edoc.ub.uni-muenchen.de/14989/ https://edoc.ub.uni-muenchen.de/14989/1/Vincendeau_Michelle.pdf Vincendeau, Michelle ddc:570,

The complete TWiV crew teams up with Welkin Johnson to explore the other AIDS epidemic, infection of monkeys with simian immunodeficiency virus, and its restriction by the cellular protein TRIM5.

Somatic hypermutation in the Ig genes is a paradigm of a site-specific, stage-specific, lineage-specific "mutator system" that generates point mutations at high rates at the Ig locus in an AID mediated and transcription dependent manner during affinity maturation of activated B-cells. The questions in the field of hypermutation today are (i) what targets the mutator factors to the Ig genes? And (ii) what are these factors? To address some aspects of these questions, I engineered and compared retroviral vectors to monitor hypermutation. Retroviral vectors combine a high transduction rate with integration at random sites within the host cell genome, thus equalizing positional effects on the reporter gene. The vectors contain a reporter gene with a premature TAG termination codon; upon reversion, a full-length fluorescent protein is expressed. The number of fluorescent cells can be easily measured in flow cytometry, and thus mutation frequencies can be determined with accuracy. I tested the green and yellow fluorescence proteins (GFP and YFP); and various proteins with red fluorescence (dsRed). Furthermore, I tested various selection markers to select for transduced cells. I found that GFP as a reporter, combined with a drug selection marker, gave the most consistent and convenient mutation rate measurements. DsRed is a good alternative to GFP, but variants with greater fluorescence intensity are needed when combined with green fluorescence measurements. To study the activity of enhancers on transcription and hypermutation, I introduced various cis-acting enhancer elements into the reporter construct. Using such constructs I found that no immunoglobulin specific sequence is needed to target hypermutation. Also, depending on their position in these ectopically expressed constructs and on the transcription rate, enhancers can have positive or negative effects on hypermutation. I also applied the indicator system to investigate whether or not mismatch repair influences AID mediated hypermutation in a non-B lymphocyte line. To do so MLH 1 expression, which is essential in mismatch repair, was regulated in a non-lymphocyte cell line that had been transduced by an AID containing vector. Whether or not MLH1 was expressed, we found no difference in mutation rates of an indicator gene. This is in contrast to activated B cells, where the absence of mismatch repair results in a reduction of hypermutation. I, therefore, conclude that in order to contribute to hypermutation, mismatch repair needs additional factors that are present in activated B lymphocytes but absent in the cell line investigated. During my cloning efforts to create DsRed containing indicator vectors, I found that a single base substitution that does not change the amino acid sequence in the gene encoding DsRed2 resulted in an increase in relative fluorescence intensity of the protein. Surprisingly, the mutated codon is slightly less favored than the original one and, therefore, would be expected to have a negative effect on fluorescence intensity, if any at all. I suggest that in highly expressed genes, the tRNA with the anticodon corresponding to a commonly used codon might become depleted. As a consequence, overall less protein is synthesized, but mRNAs using less frequent codons at some positions may be translated more efficiently.

Metastatic breast cancer is commonly thought to be incurable, but gene therapy strategies with suicide genes are emerging as a potential treatment for metastatic breast cancer. Retroviral vectors are attractive candidates for such in vivo gene therapy applications. Most of these vectors contain viral promoters that are not tissue specific. In order to specifically target malignant cells while at the same time sparing normal tissue, cancer gene therapy will need to combine highly selective delivery with highly specific gene expression, specific gene product activity and, possibly, specific drug activation. Use of cell type or tissue specific promoters would allow specificity of gene expression to be achieved. It is therefore important to identify and characterise tissue/tumour specific promoter/enhancer elements in and combination with retroviral vector systems. In this present study, the ProCon system was used to construct tissue specific vectors containing either the viral MMTV U3 region or the cellular WAP proximal promoter. It was important to characterise the vector system at the molecular level as well as investigating their targeted expression in vitro and in vivo. Validation of this system was first made with a hybrid MMTV-BAGgal vector. Here, it could be shown that the U3 from the 3' LTR could be replaced with the viral MMTV U3 region and that the hybrid vector behaves as expected at the molecular level throughout the whole transduction cycle. In transgenic mice, the ability of a small fragment of the WAP promoter to direct mammary specific expression was shown and this raised the possibility to replace the viral promoter with the WAP NRE. The replacement of viral U3 from the 3' LTR with the cellular WAP NRE showed no effect either at the molecular level or on the transduction cycle. These hybrid vectors were produced at titres ranging from 103 to 105 cfu/ml, showing that swapping of the U3 of 3' LTR with either heterologous viral or cellular sequences does not have an adverse affect on titre. Following infection and reverse transcription, the expression of reporter gene should be under the control of either the WAP NRE or the MMTV U3 region. In order to determine the activity of the WAP NRE and MMTV U3 in the context of a retroviral vector in vitro, suitable cell culture conditions were established for both primary breast tumour cells as well as established tumour cell lines. Primary human breast tumour cells and breast tumour or non-breast tumour cell lines were infected in monolayer culture and were analysed in a three dimensional cell culture system for expression of the reporter gene. Here, it was demonstrated that the MMTV U3 was mainly active in human breast tumours. The WAP NRE was also able to direct heterologous gene expression in the context of a modified retroviral vector in human breast tumour cell lines as well as in primary cells in culture, but not in other types of human tumour cells. The activity of the WAP NRE in human breast tumour cells was not necessarily to be expected, particularly since a human WAP homologue has yet to be identified. An in vivo approach involving transplantation into SCID/bg mice allowed a determination of whether or not the in vitro response is reflected in vivo and to complement the in vitro studies. This work showed that mice did indeed develop both tumours and metastases. Both the MMTV U3 and WAP NRE in the context of the ProCon system behaved in a similar manner both in vitro and in vivo. Transgenic mice were also made in order to analyse the tissue specificity of the MMTV U3 region in the context of the ProCon system. A recloned provirus DNA was used for the establishment of the mice. Expression of the MMTVProCon was mainly restricted to the mammary gland and spleen, mirroring the expression pattern of MMTV. The ability of the WAP proximal promoter and MMTV U3 region to function in the context of a retroviral vector is of interest for potential use to drive therapeutic gene expression in gene therapy strategies directed against breast cancer. Such genes could include those encoding toxic products, for example the diphtheria toxin gene or, alternatively, suicide genes allowing drug induced cell death with a bystander activity. Such strategies will be successful if ectopic expression can be avoided. In this study it was demonstrated that the proximal WAP promoter was more mammary tumour cell specific and it is therfore more suitable for use in gene therapy at this time. Whilst the MMTV U3 region, in the form used here, may exhibit ectopic expression, further experiments may identify regions or fragments that can be used without observing this phenomenon. Further, these experiments could be extended and combined with systematic delivery of hybrid retroviral vectors with a therapeutic gene either locally or into the bloodstream of xenotransplanted SCID/bg mice. In order to accomplish this, a high titre viral supernatant will be required and therefore efficient production systems will have to be developed and tested.