POPULARITY
13 episodes with opn
2 episodes with opn
3 episodes with opn
6 episodes with opn
3 episodes with opn
2 episodes with opn
2 episodes with opn
4 episodes with opn
2 episodes with opn
On a cold February night in Pontiac Michigan the dream an expecting mother would have to one day hold her son would be ripped from her when she was brutally murdered in her bedroom. The suspects would be plentiful because many had the opportunity, but this case would come down to who had the motive to murder. Patreon.com/truecrimecouple Sponsors: IQBAR IQBAR is offering our special podcast listeners twenty percent off all IQBAR products, plus get FREE shipping. To get your twenty percent off, text TCC to 64-000 MASTERCLASS See MasterClass's latest deal–at least 15% off–at masterclass.com/tcc Sources: https://law.justia.com/cases/michigan/court-of-appeals-unpublished/2009/20090825-c284096-63-284096-opn.html https://uk.practicallaw.thomsonreuters.com/Document/I007ebf67fee611d99439b076ef9ec4de/View/FullText.html?rank=0&docSource=8cf8e70f113a456abfe2b487d42da885&transitionType=Document&contextData=%28sc.Document%29 https://mdocweb.state.mi.us/OTIS2/otis2profile.aspx?mdocNumber=235484 https://caselaw.findlaw.com/court/us-6th-circuit/1429205.html https://www.courts.michigan.gov/siteassets/case-documents/uploads/Opinions/Final/COA/19960917_C173448(0049)_173448.OPN.PDF
Penultimate...Decisions are made, courses are plotted, the pieces are falling into place!Join the OPN hosts and callers as we discuss Deus-Ex-Machina and why exactly so many Discovery Crew are ready to peace out of their century!Send your voice hail to OPNSign up for the OPN NewsletterVisit our new website OpenPike.comPlease Check out our MerchSupport us on PatreonFollow @openpike on TwitterFollow OpenPike on InstagramFollow Openpike on Youtube
The SyFy Sistas join OPN to help make the sound of thunderous applause for Season 2, episode 6's "The Sound of Thunder!" The discuss the origins of Action Saru, Lord of the Rings parallels, and prosthetic caresses. Check out more from the SyFy Sistas HERESend your voice hail to OPNSign up for the OPN NewsletterVisit our new website OpenPike.comPlease Check out our MerchSupport us on PatreonFollow @openpike on TwitterFollow OpenPike on InstagramFollow Openpike on Youtube
¿Qué encontrarás en este episodio de "ConCiencia Criminal"? Fátima y Noelia repasan mes a mes lo que ha sido su 2024 laboral: casos mediáticos, conceptos criminológicos, novedades del centro... Y alguna que otra sorpresa que habíamos mantenido en secreto. ¡No te lo pierdas! La canción de la intro es: Doce Meses de Shinova Link a Noelia en Mon Interior Radio: https://www.radiomolinsderei.cat/programs/moninterior/radiomolinsderei_podcast_31495 Link a Fátima en Red Malasaña: https://open.spotify.com/episode/2FyJGZHhR3CfTu1wGpMOzR?si=e__WreWLRjOzmSNdTvvNtw Link a Fátima y Noelia en OPN opositores a Policía: https://open.spotify.com/episode/14Eg7uZANvgnWy1EAkYk04?si=uXK8cvoTTyS9Lw0uQ0h9EA Si necesitas nuestros servicios como analistas o asesores forenses llama al +34 661942896 o al +34 690761845 Si quieres participar en esta temporada escríbenos por Instagram con tu propuesta o al mail Noelia.medina@institutoforense.es ¡Felices fiestas y feliz 2025! Volvemos en enero :)
This week, we are discussing the story of Todd Stermer, who was tragically killed in a fire under circumstances that initially seemed accidental but soon raised unsettling questions. Was it a tragic accident, or was something more sinister at play? Thank you to this week's sponsors! Stop wasting money on things you don't use. Cancel your unwanted subscriptions by going to RocketMoney.com/MOMS. Treat yourself to the best bras and shapewear on the market and save up to 50% Off sitewide at honeylove.com/MOMS20 this month only. Inventory is limited and the sale ends soon so don't miss their best deals of the year. After you purchase, they'll ask you where you heard about them. PLEASE support our show and tell them we sent you. Elevate your comfort, elevate your style, thanks to Honeylove. This Holiday Season, unlock Jennifer Anniston-approved hair at lolavie.com. As our loyal listeners you'll get an exclusive 15% off your entire order when you use code MOMS15 at checkout. To advertise on the show, contact sales@advertisecast.com or visit https://www.advertisecast.com/MomsandMysteriesATrueCrimePodcast. New merch! Check out Moms and Mysteries Threadless! You can also get new episodes a day early and ad free, plus merch and more at Patreon.com/momsandmysteriespodcast Listen and subscribe to Melissa's other podcast, Criminality!! It's the podcast for those who love reality TV, true crime, and want to hear all the juicy stories where the two genres intersect. Subscribe and listen here: www.pod.link/criminality Check-out Moms and Mysteries to find links to our tiktok, youtube, twitter, instagram and more! Sources: https://www.mlive.com/news/kalamazoo/2023/03/testimony-allowed-in-retrial-of-michigan-woman-convicted-of-killing-husband-appeals-court-rules.html https://www.courts.michigan.gov/siteassets/case-documents/uploads/OPINIONS/FINAL/COA/20230316_C361326_42_361326.OPN.PDF https://mdocweb.state.mi.us/OTIS2/otis2profile.aspx?mdocNumber=757152 https://www.cbsnews.com/news/linda-stermer-husband-murder-todd-tells-her-side/ https://scholar.google.com/scholar_case?case=5147995026067897751&q=todd+stermer&hl=en&as_sdt=2006 https://scholar.google.com/scholar_case?case=2272973874759103219&q=todd+stermer&hl=en&as_sdt=2006 https://scholar.google.com/scholar_case?case=3674139640037191140&q=todd+stermer&hl=en&as_sdt=2006 https://www.newspapers.com/image/441420611/?terms=linda%20stermer&match=1 https://www.mlive.com/news/kalamazoo/2020/06/prosecutor-will-retry-michigan-woman-convicted-of-killing-husband-in-house-fire.html https://wwmt.com/news/local/linda-stermer-todd-murder-court-appeals-trial-testimony-fire-lawrence-van-buren-county-conviction-scott-leroy-jail-dardeda-gordon https://www.aol.com/court-appeals-rules-favor-woman-174300957.html https://www.wtkr.com/us-news/crime/retrial-set-for-woman-accused-of-beating-burning-running-over-husband
Ben and Adam are back! The Greatest Trek hosts are back on the OPN stage to discuss the 2002 Britney Spears/Anson Mount film “Crossroads”!Producer John was away but Jesse and Cam came to play, and take a road trip across the country and all over California with the biggest names in Star Trek podcasting! Send your voice hail to OPNSign up for the OPN NewsletterVisit our new website OpenPike.comPlease Check out our MerchSupport us on PatreonFollow @openpike on TwitterFollow OpenPike on InstagramFollow Openpike on Youtube
While Producer John is away on a secret mission, Thom Tran joins OPN to discuss Klingon holograms, mushroom ghosts, and baby heads as they unpack Discovery Season 2, episode 3: Point of Light.Join the OPN Discord!https://discord.com/invite/CXaRH5MnBhSend your voice hail to OPNSign up for the OPN NewsletterVisit our new website OpenPike.comPlease Check out our MerchSupport us on PatreonFollow @openpike on TwitterFollow OpenPike on InstagramFollow Openpike on YoutubeSend your voice hail to OPNSign up for the OPN NewsletterVisit our new website OpenPike.comPlease Check out our MerchSupport us on PatreonFollow @openpike on TwitterFollow OpenPike on InstagramFollow Openpike on Youtube
OPN continues their trip down memory lane with Captain Pike's first hair upgrade, his first Spore jump, and first contact with the people of New Eden - let's get respectful!And you didn't think we'd just let Strange New Worlds drop a new Season 3 clip without geeking all the way out about it, did you?Join the OPN Discord!https://discord.com/invite/CXaRH5MnBh ]Send your voice hail to OPNSign up for the OPN NewsletterVisit our new website OpenPike.comPlease Check out our MerchSupport us on PatreonFollow @openpike on TwitterFollow OpenPike on InstagramFollow Openpike on Youtube
It's time to put OPN on trial! John, Cam, and Jesse are joined by the delightful Yetide Badaki to discuss Star Trek Strange New Worlds and the trial of Una in “Ad Astra Per Aspera”Yetide shares her experiences on "Lost", "American Gods" and of course Star Trek. If you want to hear more from costume designer Bernadette Croft or casting directors Orly Sitowitz and Margey Simkin be sure to scroll back to their interviews in our catalog!Send your voice hail to OPNSign up for the OPN NewsletterVisit our new website OpenPike.comPlease Check out our MerchSupport us on PatreonFollow @openpike on TwitterFollow OpenPike on InstagramFollow Openpike on Youtube
It's time for an operation, and Annihilation operation, the snot pancakes have taken over the OPN crew and made us watch and discuss the Star Trek Original Series episode "Operation -- Annihilate!"Our callers were able to shine a light on some of the murkier aspects, and blinded us with their fantastic insights!!Send your voice hail to OPNSign up for the OPN NewsletterVisit our new website OpenPike.comPlease Check out our MerchSupport us on PatreonFollow @openpike on TwitterFollow OpenPike on InstagramFollow Openpike on Youtube
The final season of Star Trek Discovery is here and the OPN hosts John, Jesse, and Cam have had a chance to see the (amazing, freaking fantastic) episodes! We've unlocked this bonus coverace of episode 1 for all listeners to enjoy.Send your voice hail to OPNSign up for the OPN NewsletterVisit our new website OpenPike.comPlease Check out our MerchSupport us on PatreonFollow @openpike on TwitterFollow OpenPike on InstagramFollow Openpike on Youtube
It's war season on Open Pike Night, and actor/model David Jones joins John, Cam, and Jesse for a conversation about his work in Star Trek Strange New Worlds "Under The Cloak of War".Join the OPN hosts for a discussion of their favorite "Before they were famous" roles, "Best Trek Guest" stars, and find out which TNG Character our guest dressed as as a youngster!Send your voice hail to OPNSign up for the OPN NewsletterVisit our new website OpenPike.comPlease Check out our MerchSupport us on PatreonFollow @openpike on TwitterFollow OpenPike on InstagramFollow Openpike on Youtube
Our guest tonight is a talent who is part of both the Marvel Universe and the Star Trek universe, he knows his union details and he keeps growing as an actor from his role in the Expanse as a husband sidelined by his powerful and controlling wife to Star Trek as a husband who…um…As guy who just wants to chill with a Starship Captain and play some party games! Michael Benyaer joins the OPN crew to discuss a si-fi-soaked career, being the all-American all-Canadian, and just how crunchy those snacks were...Send your voice hail to OPNSign up for the OPN NewsletterVisit our new website OpenPike.comPlease Check out our MerchSupport us on PatreonFollow @openpike on TwitterFollow OpenPike on InstagramFollow Openpike on Youtube
ニューアルバム『Again』を9月29日（金）にリリースしたOPN。自身の作品のみならず、音楽プロデューサーとしてAnohniやThe Weekndの作品も手がけ、映画『グッド・タイム』では『カンヌ国際映画祭』にて最優秀サウンドトラック賞を受賞するなど、その動向には世界各国から大きな注目が集まっています。明後日2月28日（水）にEX THEATREで行われる『Oneohtrix Point Never（ワンオートリックス・ポイント・ネヴァー）来日公演』について、ライターの木津毅さんと深堀りしました！
Når vi taler om mental træning af atleter, handler det om at hjælpe topatleter med at holde fokus på opgaven, målet eller deres gameplan på et ekstremt højt plan. Men man behøver ikke at være topatlet for at kunne lære noget om ens hverdagsadfærd og hvordan man kan drage fordel af at kunne holde fokus på opgaven, selv på en almindelig grå mandag i november. At håndtere det mentale pres fra en hektisk hverdag eller presset under et langt løb, er en af de mest kritiske succesfaktorer, når vi taler om at stå i såvel forandringens behag som ubehag på vej til at opnå vores mål. Dette gælder både når vi skal forandre adfærd og gøre mere af noget for at nå vores mål, f.eks. at øge den distance vi vil løbe, eller gøre mindre af noget for at nå vores mål, f.eks. ikke at løbe 80 km hver dag, men kun løbe det hver anden dag.I dag skal vi altså tale om, hvordan man bruger mental træning til adfærdsforandringer, både når vi vil gøre noget mere eller mindre af noget. Spørgsmålet er: Hvordan får du noget til at ske, der ikke sker af sig selv, men som er afgørende for at du kan opnå dit mål? Til at hjælpe os med at udfolde dette emne og svare på dette spørgsmål, taler jeg i dag med søstrene Camilla og Johanne Prisak. Til daglig driver I firmaet Mental Atlet og har begge en kandidatgrad i Idræt og Sundhed med specialisering i sportspsykologi fra SDU. Og så er de begge ultraløbere.Lyt med og få:- Forståelse af adfærdsændringer: Få en klar forståelse af, hvordan mental træning kan hjælpe med at tackle adfærdsændringer, både når man ønsker at øge eller mindske en bestemt aktivitet eller vane- Anvendelighed i hverdagen: Opdag hvordan teknikkerne inden for mental træning ikke kun er forbeholdt eliteatleter, men kan anvendes af alle for at forbedre hverdagsadfærden og håndtere presset fra en travl hverdag.-3 R'er modellen: Lær om den effektive 3 R'er-model (Registrér, Release, Refokusér) og hvordan den kan hjælpe med at opretholde fokus på opgaven og målet, selv når distraktioner opstår.- Konkrete løsninger: Opnå konkrete løsninger til hver af de tre faser i 3 R'er-modellen gennem forskellige mentale træningsøvelser, herunder mindfulness, distancering og visuel koncentration.- Fremtidig praksis og refleksion med søstrene Prisaks få-det-til-at-ske-liste: Reflekter over den erhvervede viden og tænk over, hvordan man kan integrere disse teknikker i ens egen praksis for at opnå ønskede adfærdsændringer og mål.
Another bumper episode to bring us into the holiday season, as we're joined again by Jacqueline Codiga, aka DJ Horse Jeans, of music writing, promoting and podcasting fame. We've got two returning faces in Keith Rankin, who's solo project Giant Claw we've covered, on production duty for death's dynamic shoud's Faith in Persona, and our eternal object of fascination Daniel Lopatin, who we're talking about for a third (!!) time with the new OPN album, Again. Also we finally figure out what the hell is going on with all that AI business through Lee Gamble's new record Models. Next episode is our end of year wrap-up so, get hyped for that x You can find Jackie on twitter @Horse_Jeans, her writing Everywhere and her contributions to the Indieheads pod in your podcast player of choice. You can find Alexis on twitter @regresssion. You can find Boo on twitter @boocanan, and find her visual art @designbyboo and her music at boocanan.bandcamp.com.
Editor Dana Gasperine joins the OPN crew and our fantastic callers to discuss Star Trek Strange New Worlds!Did you enjoy watching:Children of the CometGhosts of IllyriaThe Elysian KingdomAd Astra Per AsperaCharadesUnder the Cloak of WarThen You've enjoyed Dana's work! Take a listen nowSend your voice hail to OPNSign up for the OPN NewsletterVisit our new website OpenPike.comPlease Check out our MerchSupport us on PatreonFollow @openpike on TwitterFollow OpenPike on InstagramFollow Openpike on Youtube
On the episode, Payton explores the mysterious misfortune surrounding the three deaths of Ed Amos's wives. Is he destined to be the world's most unfortunate man, or is there a darker and more sinister explanation? Socials and more: https://linktr.ee/bingedpod Case Sources: www.courts.michigan.gov/siteassets/case-documents/uploads/opinions/final/COA/19980818_C200898(0059)_200898.OPN.PDF http://murderpedia.org/male.A/a/amos-lowell.htm en.wikipedia.org/wiki/Lowell_Amos truecrimediscussions.blogspot.com/2017/12/lowell-amos.html core.ac.uk/download/pdf/217143256.pdf archive.ph/6wynM (Jeffersonville News and Tribune) Sinclar, Norman and Judy Turpen, “Black Widower,” Indianapolis Monthly, August 1996, pp. 94-97, 160-161 Newspapers.com sources: newspapers.com/image/972354227 newspapers.com/image/975442169 newspapers.com/image/975442214 newspapers.com/image/973090944 newspapers.com/image/976509426 newspapers.com/image/973055926 newspapers.com/image/354016229 newspapers.com/image/204357356 newspapers.com/image/441078574 Learn more about your ad choices. Visit podcastchoices.com/adchoices
Fire up your players and watch Star Trek VI along with the OPN crew and writer Davy Perez as he takes us through the influences on Star Trek Strange New Worlds S2E8 "Under the Cloak of War"Send your voice hail to OPNSign up for the OPN NewsletterVisit our new website OpenPike.comPlease Check out our MerchSupport us on PatreonFollow @openpike on TwitterFollow OpenPike on InstagramFollow Openpike on Youtube
Our guest in this episode is Tom Poland who is best-selling author of the Leadsology series of books and also Marketing with Webinars. His new book: "Get Quality New Clients for free from Other People's Networks” is launching in early November.We explore the power of email marketing, the importance of establishing strong business relationships, and the concept of leveraging other people's networks (OPN) for marketing success. Key Points:Leveraging other people's networks (OPN) through email marketing and strategic partnerships is a powerful and cost-effective way to generate new clients.Building relationships, establishing trust, and offering value are crucial in successful marketing and business collaborations.Investing in expert guidance and implementing proven strategies is essential for long-term business success.Tom previously appeared on Episode 446 of the podcast.Listen to the podcast to find out more.
Gain insights from Tom's 30 years of experience and 600 OPN partners, learning from his mistakes and understanding why certain OPN promotions work while others don't Discover the concept of Other People's Networks (OPN) and how it can be leveraged to get new clients for free in just two hours per week Understand the importance of identifying and qualifying good OPN partners, and how to approach them to set up mutually beneficial partnerships Get practical steps to get started with OPN, including setting up an email list, developing a lead magnet, and outsourcing the identification and qualification process to a freelancer Resources/Links: Get BULK New Clients Each Month In Only Two Hours Per Week FREE book. Click here: https://gettomsbook.com/ Summary Are you struggling to generate new clients for your business? Do you want a proven marketing strategy that can bring in leads consistently? Look no further! Introducing Other People's Networks (OPN), a revolutionary marketing system that can help you get new clients for free in just two hours per week. Imagine having other people promote your free offer to their email subscribers, generating a flood of new client inquiries every month. With OPN, you can tap into the power of other people's networks and leverage their email lists to reach your ideal clients. By creating a valuable free offer that features your expertise, you can entice potential clients to take action and book a time to chat with you about becoming a client. Don't miss out on this opportunity to transform your marketing and grow your business. Get your hands on the book "Other People's Networks" by Tom Poland, a multiple bestselling author and marketing expert. In this book, Tom shares his step-by-step system for implementing OPN and provides real-life case studies of its success. Check out these episode highlights: 00:10 - Discover the power of other people's networks (OPN) and how it can revolutionize your marketing strategy 00:45 - Learn how OPN can help you get new clients for free in just two hours per week 01:03 - Uncover the secrets of other people's networks and how they can generate a steady stream of client inquiries 02:32 -Real-life examples of how OPN has worked exceptionally well for businesses in various industries 04:34 - Avoid common mistakes when implementing the OPN system and maximize your results 08:09 - Understand the key differences between OPN and traditional joint ventures and why OPN offers more control and accountability 10:34 - Start now and take advantage of OPN to grow your email list and generate bulk inquiries each month 12:44 - Gain insight into the catalyst that led to the discovery of OPN and the potential it holds for your business 15:29 - Identify good OPN partners and leverage their networks to reach your target market effectively 18:12 - Take a sigh of relief as you realize that you don't have to initiate anything in the OPN process 19:08 - Get started with OPN by setting up your email list, developing a lead magnet, and enlisting the help of a freelancer 20:00 - Don't miss out on the launch of "other people's networks" by Tom Poland and learn the step-by-step system to harness the power of OPN Tweetable Takeaways from this Episode: “You've got to get yourself an email list, that's your ticket to entry into the OPN game.” -Tom PolandClick To Tweet Transcript (Note, this was transcribed using a transcription software and may not reflect the exact words used in the podcast) Uwe Dockhorn 00:10 Welcome to an interview with my mentor, Tom Poland. Hi, Tom. And it's a pleasure talking to you today. How you doing Tom Poland 00:17 Today? It's a pleasure talking with you as well. I feel like spiritual one. Well,
We're back with another solo pod due to a c*vid cancellation. In this episode we discuss Russian bath houses, the food at 'Banya 2' being surprisingly good, getting addicted to running, a cold plunge company called 'The Cold Pod', people who drink beer in the shower, film bro house parties, weird corners of TikTok disguised as Minecraft tutorials, Shallow Hal, Seinfeld, Josh getting a job as a hand model, Nathan Fielder's 'The Curse', OPN, CFCF's Night Bus mix series, haunted houses, hating scary movies, 'The Ring', Austin growing up with a well in his backyard, The Haunting of Hill House, Don's Plum, referring to things as 'too advanced', Asa-Chang & Junray's song 'Hana' and more! Josh McIntyre Austin Hutchings ---- COLD POD SUPPORT THIS PODCAST https://www.patreon.com/coldpod
WEB : https://deanderekrant.nl/ WEB: https://www.werkstandby.nl/ Bestel Dino's Nieuwe Boek : www.matrixshop.nl Bol.com : https://www.bol.com/nl/nl/p/droomstaat-van-controle/9300000156227523/?Referrer=ADVNLGOO002008J-S--&gclid=Cj0KCQjw9fqnBhDSARIsAHlcQYTLfebbRAiIHccawRoeiAqXQBhWZyaxVVMRj116ZX1FJe7min0H8dEaAmLQEALw_wcB Bruna : https://www.bruna.nl/boeken/droomstaat-van-controle-9789464810578 In deze Podcast hebben wij Jeroen Arents te gast. Hij is onder andere bekend van NIBURU,OPN en tegenwoordig werkt hij bij de Andere Krant. Er valt veel te bespreken met deze man want hij is een OG van complotten sinds Way Back… Pak je Cocktail er maar bij en geniet van deze zomerse LIVE Episode. Saluto Bestel Dino's Nieuwe Boek : www.matrixshop.nl Bronnen William Tomkins Chemtrail Video : https://rumble.com/v2r0q17-see-area51... TELEGRAM GROEP : https://t.me/dutchmatrixchannelHTTPS://WWW.MATRIXSHOP.NL
Strange New Worlds and Open Pike Night are at the end of another season! the Gorn are back and they have brought us to Episode 10 "Hegemony". Alice Wetturlund of the Treks and the City podcast joins us to discuss the terrifying vision of no foreseeable Strange New Worlds...and scary space lizards.Join us tonight as we travel to a place that looks like 1960's America that is totally unprepared for their first glimpse of the Gorn, how few special weapons it takes to arm a squad of Gorn exterminators, and which of the OPN hosts would reveal their Gorn Egg-sac status, and which would hide it until it's too late, dooming us all.Support the Entertainment Community FundCall the AMPTP - 818-995-3600Send your voice hail to OPNSign up for the OPN NewsletterVisit our new website OpenPike.comPlease Check out our MerchSupport us on PatreonFollow @openpike on TwitterFollow OpenPike on InstagramFollow Openpike on Youtube
Guest Elizabeth Brownell, AKA Rookie Hunting, on social media shares her journey going from a plant-based diet of being a vegan to becoming an adult onset hunter. Derek and Eric have a great dialog with Elizabeth about transcending an old identity and embracing a new lifestyle as a woman who decided she wanted to source her own meat. The Okayest Hunter podcast is now one of many on the Okayest Podcast Network, and Elizabeth is the host of the newest podcast to launch on the OPN, The Onset Podcast! We're happy to have Elizabeth on our growing network of podcasts - she's got a great outlook towards the hunting community. We're happy to have her among our ranks within the hunting community and a part of our outdoor podcast network! We're presented by www.Half-Rack.com - use discount code OHP at checkout for savings on all your hunting accessories! Learn more about your ad choices. Visit megaphone.fm/adchoices
Un concept mystérieux qui va vous permettre de briller en société ! "OPN" est un acronyme qui signifie "Other People Network". Autrement dit, c'est tout simplement le réseau des autres personnes autour de vous. Dans quel contexte peut-on entendre parler de l'OPN ? Dans quels secteurs d'activité peut-on utiliser l'OPN ? Comment faire de cette définition un élément actionnable pour toi en tant que Marketeur ? ➞ Découvrez-le dans cet épisode ! À écouter sans modération
Season 2 of OPN is here! Strangely enough so is season 2 of Star Trek: Strange new worlds! Get hyped for the premiere on June 15th with a great group of callers and hear the hosts struggle not to let loose any season spoilers...Send your voice hail to OPNSign up for the OPN NewsletterVisit our new website OpenPike.comPlease Check out our MerchSupport us on PatreonFollow @openpike on TwitterFollow OpenPike on InstagramFollow Openpike on YoutubeIf you have something you want to share on the Open Pike Night Stage please record an audio clip, 90 seconds or less, and send it to Openpike@gmail.comCome take your place on the Open Pike Night Stage!Most importantly, be sure to share the episode with your friends and please, leave us a review on Applepodcasts and Spotify!
Dr. Georg F. Weber, from the University of Cincinnati Academic Health Center, describes a research perspective he authored that was published by Oncotarget in Volume 14, entitled, “Crossroads: the role of biomarkers in the management of lumps in the breast.” DOI - https://doi.org/10.18632/oncotarget.28402 Correspondence to - Georg F. Weber - georg.weber@uc.edu Abstract Premalignant lesions in the breast pose a difficult decision-making problem, whether to treat proactively and accept the side effects or to engage in watchful waiting and possibly encounter a later diagnosis of invasive cancer. A biomarker or set of biomarkers to inform on the individual progression risk would be beneficial to the patient and cost-effective for the healthcare system. The gene products of tumor progression may be expressed in early non-cancerous (“premalignant”) lesions, where they are associated with a high probability for full transformation in breast cancers. One such molecule is the OPN splice variant-c. OPN-c is also present in a fraction of the premalignant lesions, where it reflects an elevated risk for progression to cancer within 5 years, regardless of the lesion's subtype. This marker has the properties needed to facilitate decisions to treat at the premalignant stage. Sign up for free Altmetric alerts about this article - https://oncotarget.altmetric.com/details/email_updates?id=10.18632%2Foncotarget.28402 Subscribe for free publication alerts from Oncotarget - https://www.oncotarget.com/subscribe/ Keywords - breast cancer premalignant lesion, biomarker, biopsy, mammography About Oncotarget Oncotarget is a primarily oncology-focused, peer-reviewed, open access journal. Papers are published continuously within yearly volumes in their final and complete form, and then quickly released to Pubmed. On September 15, 2022, Oncotarget was accepted again for indexing by MEDLINE. Oncotarget is now indexed by Medline/PubMed and PMC/PubMed. To learn more about Oncotarget, please visit https://www.oncotarget.com and connect with us: SoundCloud - https://soundcloud.com/oncotarget Facebook - https://www.facebook.com/Oncotarget/ Twitter - https://twitter.com/oncotarget Instagram - https://www.instagram.com/oncotargetjrnl/ YouTube - https://www.youtube.com/@OncotargetJournal LinkedIn - https://www.linkedin.com/company/oncotarget Pinterest - https://www.pinterest.com/oncotarget/ Reddit - https://www.reddit.com/user/Oncotarget/ Media Contact MEDIA@IMPACTJOURNALS.COM 18009220957
This is Stephen Schmidt from the Gazette Digital News Desk, and I'm here with your update for Thursday, April 20.Thursday will be windy and stormy, as cold air is coming into the area, leading to rain Thursday and a chilly Friday and Saturday. According to the National Weather Service it will be mostly cloudy in the Cedar Rapids area with a high of 68 degrees. The wind will pick up during the day, blowing as high as 20 to 25 mph and gusting as high as 35 mph. Rain chances overall for the day is 60 percent, but it appears from their analysis that rain possibilities will likely cease by 4 p.m.Crowding and capacity needs continue to stress the main University of Iowa Hospitals and Clinics, compelling administrators to expand the emergency department, reorganize existing services and — now — open a new “short-stay unit.”UIHC opened a new 30-room short-term unit Monday aimed at freeing up patient beds for sicker patients; streamlining the hospital experience for short-term patients requiring observation before heading home; and allowing providers to focus on either critical-care or short-term needs.Examples of UIHC patients who might require a short hospital stay are those needing further evaluation to determine whether they're stable enough to be released or should be admitted; patients recovering from surgery; or ER arrivals requiring observation, like those with chest pain, for example.Patients might find themselves in the short-stay unit after the operating room or a trip to the emergency room — which has become increasingly stretched in recent years.The family of a Muscatine man who went missing from Iowa City over the weekend hosted a prayer vigil Tuesday evening, and has been working with police on ongoing search efforts.Cristian Martinez, 20, had gone to Iowa City with some friends who last saw him in the alley behind Bardot Iowa, a bar at 347 S. Gilbert St., sometime before 1 a.m. Saturday. His phone had died earlier in the evening, according to the Iowa City Police Department.Martinez was reported missing Saturday after his family noticed he hadn't come home with his friends. Iowa City police investigators found security footage that showed Martinez at 12:56 a.m. in the 100 block of W. Harrison Street, walking west.Family, friends and other community members gathered Tuesday at the downtown Pedestrian Mall in Iowa City to hand out flyers and show support.The family has started a GoFundMe account to help with hiring a private investigator, and any other expenses as the search continues. As of Wednesday afternoon, the account had raised almost $10,000.Iowa City is taking the first step in exploring space and department needs of the City Hall building on Washington Street, which houses various city departments including police and fire.The city will be working with OPN Architects over the next six months on the master plan and feasibility study project, which the Iowa City Council approved as part of the consent agenda during Tuesday's meeting. Since it was on the consent agenda, there was no council discussion.OPN will review the current and projected space and needs of departments — including whether it would be possible to relocate the police department to a stand-alone building.
Welcome to Quest Me! Your weekly Star Wars podcast taking you through Legends and New Canon! This time around we are covering the new season of the hit Disney+ series, The Mandalorian!On this weeks episode Jesse from Open Pike Night joins Phil and Josh to talk about Chapter 18 of The Mandalorian: The Mines of Mandalore!If you didnt catch our newest breakdown of the 2nd book in the Thrawn trilogy: Dark Force Rising, make sure to check that outRIGHT HEREA Bar Far Away Recipe2 oz gin0.5 oz lemon juice0.5 oz sage simple syrup*12-15 blueberriesMuddle blueberries at bottom of shaker tin.Add gin, lemon juice, simple syrup, and ice. Shake and strain over fresh ice in a rocks glass. Top with ginger beer.Garnish with fresh sage leaves and additional blueberries.“To make the sage simple syrup, you just bring one cup of sugar, one cup of water, and a dozen sage leaves to a boil in a saucepan for one minute. Then remove from heat and let steep for a half hour. Remove leaves—squeeze 'em out—and store the syrup in a sealable container. It will last in the refrigerator for a couple of weeks.” -Kevin HearneFind Kevin Hearne HEREFollow Open Pike Night And Jesse HEREVisit the OPN website HEREFind all the Open Pike Night Merch seen on the live show HEREFollow Justin on Instagram HEREFollow Phil on Twitter HERETake a peek at Phils Blog, "The Swords of the Ancients" HEREFollow Quest Me on twitter @QuestMePodcastOn Instagram HEREOn Facebook HERESubscribe to our Youtube channel HERE To see our live streamsEmail us: Questmetma@gmail.comNext week we have Scuttlebutt Ro from the Scariff Podcast Joining us to talk about Chapter 19 of The Mandalorian! We do not own the rights to the clips or Music used in this podcast.
New Music & Videos, RIP Double TT (Tristan Thompson)'s Mom, get well soon Damar Hamlin. NFL Wildcard Rundown & NBA Run to Allstar Weekend and NdABuilding tonight we NdACity, OPN and UGR network sign up sheet. --- Send in a voice message: https://anchor.fm/ndapostpodcast/message
Being an entrepreneur can feel rewarding & flexible. Yet also lonely & stressful. How do you manage taking care of yourself while also taking care of a business, clients & other expectations of life? On this episode Joi is joined by father, entrepreneur, owner of Opn & mental health advocate Dwight Peters (IG: @dwightlpeters/@get_opn). They discuss how being an entrepreneur can impact your mental health & some ways to combat it. If you or someone you know is in crisis or struggling with suicidal thoughts, please call or text 988. You don't have to do life alone! Happy Holidays & see you in 2023!
Forfatteren Karin Michaëlis blev i 2022 fremhævet som en af Danmarks vigtigste forfattere på Klassikerdagen. Hun er født for 150 år siden. Opnåede internationalt anerkendelse for 100 år siden. Blev hyldet af europæiske koryfæer som Bertolt Brecht og Thomas Mann. Men selvom hun stadig er kendt i udlandet , så blev hun efterhånden nærmest fuldstændig glemt hjemme. I 2022 blev hun valgt til at være årets klassiker af komiteen Klassikerdagen, som hvert år sætter fokus på en dansk forfatter. I årene før havde det været Agnes Henningsen, Ole Lund Kierkegaard og Inger Christensen. På det tidspunkt var der allerede begyndt at blive mere bevidsthed om den glemte store danske forfatter – bla. igennem Karin Michaëlis-selskabet der var blevet stiftet af en række forfattere og litteraturfolk. Ved et foredrag på Hovedbiblioteket i september 2022 fortalte forfatteren Merete Pryds Helle og litteraturforsker Karen Klitgård Povlsen om Karin Michaëlis. Se litteraturliste Tilrettelagt af Claus Vittus Københavns Biblioteker Musik: Mountain Air, It´s Been So Long og Seven Falls/Epidemic Sound
For this week's Record Club, Jake and Ryley have a blast breaking down legendary modern electronic mastermind Daniel Lopatin's 2015 masterpiece as Oneohtrix Point Never, the abrasive and heavily conceptual Garden of Delete. Join us as we unpack the strange and affecting concept and ideas behind this remarkably unique tentpole of 2010s electronica, and how it threads the needle between the industrial rock of the 90s and modern sonic sensibilities, while still being refreshingly unfashionable in its own exciting ways. Let us know what you think of this record and OPN's music in the comments below. Watch the video version of this episode to support the channel on YouTube.
Scott from @Spyhards joins the OPN crew to host the stage while everyone celebrates the launch of Strange New Worlds!The guests on stage tonight bring their deep thoughts and hot takes ranging from how sexy the opening credits are, to how sexy Anson Mount's Pike is, to how sexy Spock is...to how sexy...DO tey talk about anything but the sexiness of this show?!Of course! Gritty Tribbles, Shitty Nacelles, Mouthing Off to a Superior Officer, "Getting Political", and the inevitable comparison to other Trek episodes and premieres.Thanks to our guests on stage tonight!@rosalita9899@QuestMeTMA@SiriusCreator@abbysommer@cheapseatcast@tribblespodcast@durassisterspod@ScifiRemnant@Petertrek1@ontherock81@BacklookcinemaAnd to see the beautiful animatic by @SiriusCreator go here: https://www.youtube.com/watch?v=4lexrkqVhGo
Greg wraps up his chat with Jeffery Potvin, the Founder & CEO of Open People Network. OPN is a group of entrepreneurs and business pros who help startups with business fundamentals, technical and tactical outcomes that help build future leaders. In part two, Jeffery talks about the importance of founders seeking out mentors, why he thinks robotics will explode in the next 3-5 years, which changes can provide the biggest impact on the startup investing ecosystem.
Jeffery Potvin is Greg's guest on the podcast. Jeffery is the Founder & CEO of Open People Network, a group of angel investors helping accelerate the growth through The Supporters Fund and Pitchit Program. In part one, Jeffery shares what inspired him to become an angel investor, what differentiates OPN from other investment groups, and what he's doing to improve the entrepreneurial failure rate.
Luke Winchester from Merewether Capital and Claude Walker from A Rich Life go in-depth and stock specific. Stocks covered: PH2, NXS, CTT, OPN, SES, BMT, PYG, PWR, DUR, ERD. Our stock of the day is Smartgroup (SIQ). See acast.com/privacy for privacy and opt-out information.
Myra, Dei & Mojo discuss the Weeknd's fifth album is both a sonic and thematic follow-up to After Hours. It also has him riding the cinematic and retro synth-pop sounds first explored in 2016's Star Boy to its zenith. This is immortalized by the A-list personnel behind the new project, from Swedish pop maestro Max Martin and EDM trio Swedish House Mafia to U.S. producer and film composer Daniel Lopatin, who goes by Oneohtrix Point Never, or OPN. You could argue that Dawn FM is merely a flowering of ideas that were there all along. House of Balloons creates chilly R&B out of samples of Beach House, and Aaliyah and elsewhere seems to nod to the moody drums & bad vibes of Phil Collins classics and the bedroom antics of '90s R&B legends like Jodeci. Elsewhere, pop hitmaker Max Martin takes guitars and synths and transforms them into disco-inflected bangers such as the album's lone single, "Take my Breath." The rhythm guitars resemble a mix of '80s funk and Daft Punk on "Sacrifice," which was built atop a sample of Alicia Myers' 1981 track "I Want to Thank You." And on "Less Than Zero," a tender acoustic guitar opens a new-wave lane that we've never seen the Toronto singer explore before, a rare moment of sonic fun (it's also one of two tracks on this entire album that is in a primary key). There was even be a global early, live broadcast today on Amazon Music for those who are extra eager to share the listening experience with fans across the world. As we count down the hours until the official drop at midnight, I am extraordinarily thankful for The Weeknd's spontaneity, for I am sure we are about to experience music history in the making. Listen to The Weeknd on Apple music. Also, they discuss. Gunna has come a long way, solidifying himself as one of the most prominent hitmakers coming out of Atlanta. Though he didn't release much music last year, he appeared on several other compilations like Young Thug's Punk, Pop Smoke's Faith, Doja Cat's Planet Her, and many others. Now, it's time for young Wunna to shine. Additionally, he further explores the themes of people he can no longer trust and how that has changed him. In the chorus, he raps, "You know b*****s talk, but you were the boss, so we keep our business private." He backs up these lyrics by acknowledging how he has helped others with "I got some slimes out a beehive, I know they are dying 'bout it." Overall, this song is a coming-of-age track for Gunna and is a perfect opener for the final chapter of the Drip Season series. On the next track, "poochie gown," there is a lot of anticipation and beef surrounding this track. However, it seems that, while there may be a lot more behind the scenes of this track, the main point that comes to attention is his apparent diss at Freddie Gibbs—produced by Wheezy, this song bass-heavy track with Gunna delivering fast flows. Gunna drops the bar in the song, "Hang above the rim. I can't fuck with Freddie Gibbs." He even backed this up on Twitter with a diss that would eventually evolve into a series of tweets from Gibbs. --- This episode is sponsored by · Anchor: The easiest way to make a podcast. https://anchor.fm/app
In this podcast, I talk about startups and the importance of a mentor in a startup with Jeffery Potvin.Jeffery Potvin, an angel investor in multiple regions who have Invested in 55+ companies. He is a member of the York, Spark & GTAN angels & screening committees while being the driving force behind Open People Network (OPN)! OPN is a group of angel investors helping accelerate the growth of early-stage startups through The Supporters Fund and Pitch it Series.Jeffery is a lifelong entrepreneur with a proven track record of building companies and reinventing existing businesses. Working with a list of great startups to enterprise clients over the years. Jeffery is a mentor, coach and loves to climb mountains!RESOURCESLinkedin:linkedin.com/in/jefferypotvin★★★ Are you someone who is: ★★★ About to start a business and needs guidance, Already running a business and wants to scale faster, Not satisfied with your current job and want to change your career, Very much satisfied with your professional career but want to stop working for others and gain financial freedom, Already enjoying financial freedom but want to create more impact in your community and in the world. If you identify with any of these concerns, I am pleased to invite you to check out these 3 solutions carefully designed to address your desires. These offers are currently in-demand and are producing amazing results:
On this episode Joi is joined by no other than THEE Marvin Toliver (IG: @mtoliver_lcsw)! We love that Marvin, along with his @melanatedsocialwork brothers believe in being both seen & heard. We need that especially in the Social Work field where there can be so many mixed feelings regarding speaking up for yourself. Interested in joining Tiyanna (IG: @tspeaksnyc) & Joi's First Annual Mental Health Provider Gift Exchange? Be sure to DM Joi by 11/15 your name and email address to be added to the list. Check out their Instagrams for more information. Mentioned in this episode: Shout out to Opn. for coming to disassemble Joi's couch from her office! Opn. (IG: @get_opn) is a Black owned handyman company based in Harlem. Reach out to them for your needs regarding furniture assembly/disassembly, TV mounting & cell phone repair to name a few.
Olá, seja muito bem-vindo ao StandardsCast EP #109 ALL FLEET. Neste episódio com o pessoal do CDV continuamos a conversa com Diogo Youssef (Gerente de Despacho de Voo), Edu Pongeluppe (Coordenador de Despacho de Voo) e Diogo Melo (Coordenador de Suporte Operacional) sobre o Extra Fuel da navegação, diferença entre as siglas ATC, ATC HOLD DEST, WXX, WX HOLD DEST, OPN, ADD e ADD FOB, assim como sobre o conceito de MFOD, combustível de arremetida e alternativa.] Em caso de dúvidas, críticas ou sugestões, envie um e-mail para standardscast@voeazul.com.br. Este Podcast foi produzido pela Diretoria de Operações da Azul Linhas Aéreas. Em caso de divergência entre qualquer assunto técnico abordado e os documentos oficiais, os documentos prevalecerão. Todos os direitos reservados.
Olá, seja muito bem-vindo ao StandardsCast EP #108 ALL FLEET. Neste episódio com o pessoal do CDV recebemos Diogo Youssef (Gerente de Despacho de Voo), Edu Pongeluppe (Coordenador de Despacho de Voo) e Diogo Melo (Coordenador de Suporte Operacional) para conhecer um pouco mais sobre o Extra Fuel da navegação, diferença entre as siglas ATC, ATC HOLD DEST, WXX, WX HOLD DEST, OPN, ADD e ADD FOB, assim como sobre o conceito de MFOD, combustível de arremetida e alternativa. Em caso de dúvidas, críticas ou sugestões, envie um e-mail para standardscast@voeazul.com.br. Este Podcast foi produzido pela Diretoria de Operações da Azul Linhas Aéreas. Em caso de divergência entre qualquer assunto técnico abordado e os documentos oficiais, os documentos prevalecerão. Todos os direitos reservados.
O método OPN é o melhor treinamento do Brasil para profissionais que atuam em farmácias e drogarias. Descubra a tríade do sucesso! Lista de espera do Método OPN: https://farmaceuticoresponde.kpages.online/listadeespera
BIO: Jeffery Potvin is an angel investor in multiple regions and has invested in 55+ companies. He is a member of seven angel groups and screening committees while being the driving force behind Open People Network (OPN)! OPN is a group of angel investors helping accelerate the growth of early-stage startups. STORY: Jeffery’s company had been working on and off with this startup for about two years. When the startup came back with a product and an investor, Jefferey took interest, and even though he had some doubts, he trusted the startup. It turned out his gut was right because the investor never held his part of the deal. LEARNING: Walk away from anyone trying to pressure you to close a deal and have the proper documentation in place before you sign the contract. The ultimate validation comes from your customer. “Look for resources, educate yourself, learn and dive right into it. Build that product, then find that angel investor.” Jeffery Potvin Guest profilehttps://www.linkedin.com/in/jefferypotvin/ (Jeffery Potvin) is an angel investor in multiple regions and has Invested in 55+ companies. He is a member of seven angel groups and screening committees while being the driving force behindhttps://opn.ninja/ ( Open People Network) (OPN)! OPN is a group of angel investors helping accelerate the growth of early-stage startups throughhttps://supportersfund.com/ ( The Supporters Fund) and Pitchit Series. Jeffery is a lifelong entrepreneur with a proven track record of building companies and reinventing existing businesses. He has worked with a list of great clients, from startups to enterprises, over the years. Jeffery is a mentor, coach and loves to climb mountains. Worst investment everJeffery worked with a company for about two years on and off, helping them through their journey. An opportunity came up to invest in this new emerging company that had some great IP. The company had found an investor that would help them with the production. The investor was going to contribute considerable funds too. Getting deeper into the business ideaJeffery went through this process of doing a deep dive and analysis around the business. He requested that he meet with the investor who would pick the ball up and put a lot of money in to make this company successful. Jeffery met the investor, and as he started to pick their brain and learn more about them, he asked them questions because he had doubts. However, he never admitted his misgivings; he just kept going forward. Ignoring hisJeffery’s gut still told him that something was not right. But because he had been working with the startup for such a significant amount of time, he trusted that they had picked the right investor. Jeffery did not want to slow down the progress, so he ignored his gut, did all of the analysis, came back, and signed off on the deal. The truth comes outJeffery signed everything off, and everything was good. About six months into the project, when the final handoff was supposed to happen, the unforeseen happened. Once everything was solidified and sorted out, the investor ended up having creditors coming after them, and they went bankrupt. Everything they had worked hard for went down the drain just because of one person. If only Jeffery had listened to his gut. Lessons learnedWalk away from anyone trying to pressure you to close a dealIf there is any panic or pressure to close a deal, walk away. There’s a reason they’re putting that pressure on you. They’re probably in debt or something else. There’s always a problem when it’s high pressure. Nothing needs to be solved in five minutes; you should always have time to think. Be wary of third party validationhttps://myworstinvestmentever.com/ep370-rashmi-shetty-when-you-let-go-of-external-validation-the-world-opens-up/ (Validating a product) is very important but be careful when the owners want to bring in different people to validate your problem. If they cannot validate it themselves,...
BIO: Jeffery Potvin is an angel investor in multiple regions and has invested in 55+ companies. He is a member of seven angel groups and screening committees while being the driving force behind Open People Network (OPN)! OPN is a group of angel investors helping accelerate the growth of early-stage startups. STORY: Jeffery's company had been working on and off with this startup for about two years. When the startup came back with a product and an investor, Jefferey took interest, and even though he had some doubts, he trusted the startup. It turned out his gut was right because the investor never held his part of the deal. LEARNING: Walk away from anyone trying to pressure you to close a deal and have the proper documentation in place before you sign the contract. The ultimate validation comes from your customer. “Look for resources, educate yourself, learn and dive right into it. Build that product, then find that angel investor.” Jeffery Potvin Guest profilehttps://www.linkedin.com/in/jefferypotvin/ (Jeffery Potvin) is an angel investor in multiple regions and has Invested in 55+ companies. He is a member of seven angel groups and screening committees while being the driving force behindhttps://opn.ninja/ ( Open People Network) (OPN)! OPN is a group of angel investors helping accelerate the growth of early-stage startups throughhttps://supportersfund.com/ ( The Supporters Fund) and Pitchit Series. Jeffery is a lifelong entrepreneur with a proven track record of building companies and reinventing existing businesses. He has worked with a list of great clients, from startups to enterprises, over the years. Jeffery is a mentor, coach and loves to climb mountains. Worst investment everJeffery worked with a company for about two years on and off, helping them through their journey. An opportunity came up to invest in this new emerging company that had some great IP. The company had found an investor that would help them with the production. The investor was going to contribute considerable funds too. Getting deeper into the business ideaJeffery went through this process of doing a deep dive and analysis around the business. He requested that he meet with the investor who would pick the ball up and put a lot of money in to make this company successful. Jeffery met the investor, and as he started to pick their brain and learn more about them, he asked them questions because he had doubts. However, he never admitted his misgivings; he just kept going forward. Ignoring hisJeffery's gut still told him that something was not right. But because he had been working with the startup for such a significant amount of time, he trusted that they had picked the right investor. Jeffery did not want to slow down the progress, so he ignored his gut, did all of the analysis, came back, and signed off on the deal. The truth comes outJeffery signed everything off, and everything was good. About six months into the project, when the final handoff was supposed to happen, the unforeseen happened. Once everything was solidified and sorted out, the investor ended up having creditors coming after them, and they went bankrupt. Everything they had worked hard for went down the drain just because of one person. If only Jeffery had listened to his gut. Lessons learnedWalk away from anyone trying to pressure you to close a dealIf there is any panic or pressure to close a deal, walk away. There's a reason they're putting that pressure on you. They're probably in debt or something else. There's always a problem when it's high pressure. Nothing needs to be solved in five minutes; you should always have time to think. Be wary of third party validationhttps://myworstinvestmentever.com/ep370-rashmi-shetty-when-you-let-go-of-external-validation-the-world-opens-up/ (Validating a product) is very important but be careful when the owners want to bring in different people to validate your problem. If they cannot validate it themselves,...
This is how Tom introduces himself: Gidday, I'm a multiple best-selling author and I've had the privilege of sharing speaking platforms with the likes of Michael Gerber of E-Myth fame, Richard Koch from the 80-20 Principle, Brian Tracy and many others. I work from home which sits on the white sands of little Castaways Beach in Australia and am blessed to have clients in 27 cities and 15 time zones right around the world. I'm voluntarily married to a uber-pretty German Frau and because I'm from New Zealand originally, we're known locally as the Kiwi and the Kraut. In this episode, Virginia and Tom talk about Tom’s business background How to change your mind about work How to use OPN to build predictable lead generation The psychology of humans and reciprocity 4 Levels of psychological allure Key takeaways from this episode: Shift from you HAVE to work to “I WANT to work!” Get referrals to people who have a network of potential customers If someone thinks you WANT something from them, you’ll create aversion and avoidance Good marketing make someone an offer for the thing they are already looking for Do your due diligence! If all you do is give, you’ll likely feel used and abused Get a free gift from Tom at: www.leadgendemo.live Tom would like to meet Bill Baren
Had a big show! Special Guest Dean Blandino, Fox Sports Rules Analyst and former VP of NFL Officiating, joins the show and talks:- Being the replay official for two Super Bowls- Thoughts on replay in sports- What's a catch?
Leveraging Other's people network or OPN is one of the thing growth hackers or digital marketers use on day to day basis, but understanding it is very important in our life, that is why I am making this podcast for my audience so that they can understand it.
Co-Founders of RaveOn Sports, Brittany Harris and James Clark stop by the show and discuss:- Kentucky Basketball- March Madness- NBA
Had Nithin Ramachandra, host of The NR Hour Podcast on the show and we talked:- Knicks- Cowboys- Yankees
Mike Gill of 97.3 ESPN Radio joins the show as we talk:- 76ers Season- NCAA Tournament- Eagles future and off-season
We get a little high-tech this week. Techdirt founder Mike Masnick joins us to explain how Section 230 actually works, and how it was somewhat unusually applied in a recent Second Circuit case. (Mike’s explanation may differ from what a multitude of “experts” have recently been saying in Congress and elsewhere.) And have you seen any drones above your house? IJ attorney Josh Windham tells a story about a drone that flew above someone’s property, and thereby committed a “search” under the Fourth Amendment (well, the government in charge of the drone did). This very interesting case from the Michigan Court of Appeals allows us to talk about drones, reasonable expectations of privacy, and whether the air above your house is an “open field.” Transcript forthcoming: https://ij.org/wp-content/uploads/2021/03/short-circuit-167.pdf Register here for the April 20th online event on the 150th anniversary of Section 1983, https://ij.org/event/outragelegislation/ New IJ drones case, https://ij.org/case/north-carolina-drones/ Greg McNeal, Police Drones, the Fourth Amendment, and Drone Trespass, https://gsmcneal.com/police-drones-the-fourth-amendment-and-drone-trespass-a-new-case-in-michigan/ Domen v. Vimeo, https://www.ca2.uscourts.gov/decisions/isysquery/01ae9011-839d-4391-a5f8-59d94d49c45b/3/doc/20-616_opn.pdf Long Lake Township v. Maxon, http://publicdocs.courts.mi.gov/OPINIONS/FINAL/COA/20210318_C349230_47_349230.OPN.PDF Long Lake Township dissent, http://publicdocs.courts.mi.gov/OPINIONS/FINAL/COA/20210318_C349230_48_349230D.OPN.PDF Mike Masnick, https://www.techdirt.com/user/mmasnick Josh Windham, https://ij.org/staff/joshua-windham/ Anthony Sanders, https://ij.org/staff/asanders/ iTunes: https://podcasts.apple.com/us/podcast/short-circuit/id309062019 Spotify: https://podcasters.spotify.com/podcast/1DFCqDbZTI7kIws11kEhed/overview Stitcher: https://www.stitcher.com/podcast/institute-for-justice/short-circuit Google: https://play.google.com/music/listen?u=0#/ps/Iz26kyzdcpodkfm5cpz7rlvf76a Newsletter: ij.org/about-us/shortcircuit/ Want to email us? shortcircuit@ij.org
Faça parte do meu canal do Telegram para não perder nenhum conteúdo: https://t.me/farmaceuticorespondeoficial Lista de espera do Método OPN: https://farmaceuticoresponde.kpages.o... Me siga também no Instagram: https://www.instagram.com/farmaceutic... (@farmacêuticorespondeof)
Had Colin Thompson, Carolina Panthers Tight End, on the show and we talked:- What it was like to play in the XFL- The feeling of catching a touchdown in the NFL- Teddy Bridgewater rumors
Denny Lennon, host of Sports Stories with Denny Lennon, joins the show and we have a great conversation while talking about:- His show and his favorite interviews- Venice Backyard Championships- Lakers- Rams
Max Starks, 2-Time Super Bowl Champion with the Pittsburgh Steelers joins the show.Topics:- Favorite NFL memory- Favorite Teammate- Thoughts on Big Ben- What it was like playing/winning Super Bowls
Former MLB Pitcher and Coach and owner of the pitcher development company Pitching Coach Pro, Steve Schrenk, joins the show and we talk:- Phillies Pitching and Season Outlook- State of MLB Pitching- How MLB recovers fans after COVID
Andrew Bogusch of CBS Sports Radio and WFAN returns and we talk about:- J.J. Watt and the Super Bowl- NCAA op teams and Big Ten basketball- NBA, Nets Big 3 & surprise of the Knicks - The Wild NBA West
Tara Lynn was successful at her job and as a mother. Her husband, Stephen, didn’t like the fact that she wasn‘t at home as much as he thought she should be, due to her job involving a good amount of traveling. She was the breadwinner of the family. In my opinion, he seemed jealous of that. He tried to control her in different ways. On Valentines Day, he reported that Tara had been missing for five days. Stephen knew exactly where she was while he played the distraught husband on the news.Here is a link that has every domestic abuse hotline number in every country! Please call one of these numbers if you or someone you know needs help:https://www.mysticmag.com/psychic-reading/domestic-violence-resource-guide/Podcast Promo: Across the VeilPatreon: https://www.Patreon.com/ReverieTrueCrime Merch: https://www.teepublic.com/stores/reverie-true-crime-store?ref_id=17247Amazon Wishlist: https://www.amazon.com/hz/wishlist/ls/2QY9RANJ59KH3?ref_=wl_shareFacebook: https://www.Facebook.com/ReverieTrueCrimeInstagram: https://www.Instagram.com/ReverieTrueCrimeTwitter: https://www.Twitter.com/ReverieCrimePodContact: ReverieTrueCrime@gmail.comSOURCES:https://www.theoaklandpress.com/news/stephen-grant-au-pair-told-police-of-minimal-affair/article_ae0ce647-a321-5c47-b21d-4ff3e0795b3b.html https://www.foxnews.com/story/german-teen-au-pair-says-she-had-sex-with-accused-killer-on-eve-of-wife-slaying-dismemberment https://www.clickondetroit.com/news/2007/12/13/testimony-continues-in-4th-day-of-grant-trial/ https://www.redorbit.com/news/business/1178603/grant_shared_bed_with_au_pair_on_night_before_his/ https://www.foxnews.com/story/au-pair-stephen-grant-betrayed-used-me https://www.nbcnews.com/id/wbna23409987 https://www.fox2detroit.com/news/a-look-back-at-the-steven-grant-murder-case-of-wife-tara-after-10-years https://www.detroitnews.com/story/news/local/macomb-county/2017/09/30/tara-grant-legacy-murder-ten-years-later/106164920/ https://truecrimedaily.com/2017/05/26/stay-at-home-dad-murders-business-executive-wife-dismembers-and-disperses-body-parts/ https://en.wikipedia.org/wiki/Murder_of_Tara_Lynn_Grant https://www.freep.com/story/news/local/michigan/macomb/2015/03/27/stephen-grant-murder-mutilation-michigan-macomb-judge-david-lawson/70554938/ https://www.theoaklandpress.com/news/breaking-news-stephen-grants-graphic-hospital-bed-confession/article_4f4d892c-84e7-5ac3-8789-b6d0e54657f4.html https://www.lansingstatejournal.com/story/news/local/michigan/2014/08/28/tara-grants-sister-speaks-years-slaying/14726157/ https://www.cbsnews.com/news/cops-husband-admits-to-horrific-murder/ https://www.sootoday.com/local-news/more-developments-in-stephen-grant-case-114155 https://www.clickondetroit.com/news/2007/12/13/read-blog-on-stephen-grants-trial/ https://www.macombdaily.com/sports/jury-finds-stephen-grant-guilty-of-nd-degree-murder/article_b88750fc-f7f3-5b67-ab4f-f89615f9def5.html https://www.foxnews.com/story/jury-selection-begins-in-trial-of-stephen-grant https://law.justia.com/cases/michigan/court-of-appeals-unpublished/2009/20091006-c284100-70-284100-opn.html http://publicdocs.courts.mi.gov:81/opinions/final/coa/20091006_C284100_70_284100.OPN.PDF https://www.clickondetroit.com/news/2007/06/06/stephen-grant-bound-over-for-trial/ https://www.clickondetroit.com/news/2007/12/12/testimony-continues-wednesday-in-grant-trial/ https://mylifeofcrime.wordpress.com/tag/2007/page/18/ https://abcnews.go.com/US/LegalCenter/story?id=3039832&page=1https://www.clickondetroit.com/news/2017/02/18/10-years-later-remembering-details-of-tara-grants-disturbing-death/ https://detroit.cbslocal.com/2017/02/09/gruesome-macomb-county-murder-10-years-ago-today-looking-back-at-the-stephen-grant-case/ https://murderpedia.org/male.G/g/grant-stephen.htm https://wickedwe.com/stephen-grant/
Jeffery (also known as Jeff or JP) has been “Open” with startups and founders for a long time, dating back to his days as an internal IT / dev guy for the Loblaw’s grocery chain. In those days, Jeffrey used his server room to meet with startups that “pitched” their ideas for the Loblaw ecosystem; fast forward a few years and now Jeffery and his Open People Network (“OPN”) team use Zoom, YouTube, Podcasts and social media to bring entrepreneurs and investors together. Jeffery did get on the end of the Kaboose, drove down Route 15 and made a HardBoot before he landed on the OPN gig! To learn more about the work of Open People Network, click on one or more of the links below: §§§ Open People Network website | https://opn.ninja/ Open People Network on Instagram | @openpeoplenetwork Open People Network on Twitter | https://twitter.com/Opeoplenetwork Open People Network on Facebook | https://www.facebook.com/Opeoplenetwork/?_rdc=1&_rdr Open People Network on YouTube | http://bit.ly/2MZzdGj §§§ Jeffery Potvin on LinkedIn | https://www.linkedin.com/in/jefferypotvin/ --- Send in a voice message: https://anchor.fm/allen-wazny/message
Jeff Bunting, VP at Corum Group, leads a discussion with investors centered around which strategies get funded and which get forgotten. Guests include speakers from Silicon Valley in Your Pocket, UCI Law, Expert DOJO, OPN, and Dreamers VC. Check out other podcast episodes, webcasts, and videos at WFS.com.
In this Episode of the PHYT For Tomorrow Podcast I talk with Occupational Therapist and PHYT team member Cara Barton. Cara lives the active lifestyle by participating in Crossfit, Cyclocross, horseback riding, and nutrition, Here we talk about how she got into all these activites and how they helped shaped her decision to get into rehab, dry needling, nutrition, and how this is able to help people stay active. If you want to hear our discussion about crossfit programming and recovery, dry needling, and nutrition this is worth a watch!To schedule a custom assessment or Telehealth visit with Cara or one of the other team members visit https://www.phytforfunction.com/bookAlso check out OPN her Occupation Performance and Nutrition Company at https://www.opandnutrition.com
Tom Poland, renowned entrepreneur and bestselling author of the Leadsology series and Marketing with Webinars, shares his system to marketing with webinars by collaborating with influencers. Tom Poland's Lessons to Partner with Influencers through JV webinars During the conversation, Tom reveals: The power of "OPN" to share your message in a grander way and land new clients The four R's to connect with business partners The system to create a constant stream of client consultations on your calendar The Prius factor to build connections with influences What to do when you feel you should apply a certain marketing strategy that everyone else seems to be applying The reason he doesn't favor common marketing strategies such as Facebook ads and blogging, and what really works instead to get new clients The two key streams of leads for his business, and how careful documentation of each step in the process and a team of contractors allowed him to create a seamless process to grow his business About Tom Poland Time Poland is a multiple best-selling marketing author. Over the past 41 years he had started and sold five businesses, taking three of them international and has lead teams of over 100 people with annual revenue of more than 20 million dollars. The new book Marketing with Webinars reveals his unique webinar marketing method that has helped thousands of organizations globally enjoy the fulfillment and prosperity that comes with a weekly flow of high-quality, inbound, new client inquiries. Learn more about Tom at https://www.leadsology.guru/ Check out Tom's new book, Marketing with Webinars at https://www.cloriskylie.com/marketingwithwebinars Take Tom's Five Hour Lead Generation Challenge www.fivehourchallenge.com Join Tom's Facebook group: www.facebook.com/groups/leadsology/
Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2020.06.23.168054v1?rss=1 Authors: Levine, J. N., Schwartz, G. W. Abstract: In the mouse, retinal output is computed by over 40 distinct types of retinal ganglion cells (RGCs) (Baden et al., 2016). Determining which of these many RGC types project to a retinorecipient region is a key step in elucidating the role that region plays in visually-mediated behaviors. Combiningretrograde viral tracing and single-cell electrophysiology, we identify the RGC types which project to the olivary pretectal nucleus (OPN), a major visual structure. We find that retinal input to the OPN consists of a variety of intrinsically-photosensitive and conventional RGC types, the latter a diverse set of mostly ON RGCs. Surprisingly, while the OPN is most associated with the pupillary light reflex (PLR) pathway, requiring information about absolute luminance, we show that the majority of the retinal input to the OPN is from single cell type which transmits information unrelated to luminance. This ON-transient RGC accounts for two-thirds of the input to the OPN, and responds to small objects across a wide range of speeds. This finding suggests a rolefor the OPN in visually-mediated behaviors beyond the PLR. Copy rights belong to original authors. Visit the link for more info
話は震災のときの音楽体験からなぜか、若林が偏愛するシン・リジーへ。そしてジェイ・エレクトロニカの問題作の話題から、有事にあっても影響を受けない音楽のすごさに、改めて感嘆してしまうこと。 00:00 | 震災とジム・オルーク 02:00 | シン・リジーのサウダージ 04:50 | ダブリンのヒップホップに萌える 07:10 | OPNが心配 10:55 | ウィークエンドとシンセウェーブ 13:03 | 声楽の新しい方向性 15:57 | テクニカルデスはよく寝れる 18:00 | LAビートシーンのなごみ感 20:25 | 黄昏のジェイ・エレクトロニカ 23:55 | 有事に負けない音楽 出演・撮影： 若林恵 小熊俊哉 録音・編集補佐： 宮野川真（Song X Jazz） 収録日： 2020年4月2日 場所： 黒鳥福祉センター 企画・制作 blkswn publishers
Data er din konkurrencefordel. Opnå en forbedret brug af data gennem bedre datakvalitet, kortere cyklustid og overlegen datahåndtering med brug af Hitachi Vantara DataOps. Hitachi Vantara fremmer innovationen og styrker konkurrenceevnen mellem mennesker, processer og teknologi og gør dig dermed i stand til fuldt ud at styre, berige og transformere og levere indsigt i hele din virksomhed. Muligheder som kan øge din kundeforståelse, kundetilfredshed, forretningsoptimere og give dig et bedre grundlag for at træffe bæredygtige beslutninger i fremtiden.
Introduction: Alexander Caillet is renowned for his pioneering approach to team coaching and his state of mind research with organisational Leaders. He is an organisational psychologist, consultant, executive coach and team coach, working internationally. Alexander is CEO of Corentus, a coaching and consulting organisation dedicated to transforming teams. He is also faculty at Georgetown University, He is a frequent international speaker on subjects such as change, Teams, Team Coaching and Leadership. Alexander has co-authored a mini book called Corentus Team Coaching, a fascinating read. Alexander is a dual citizen of France and the US and he lives in Boston with his wife and 3 beautiful daughters. Podcast episode summary: In this episode Alexander Caillet communicates with enormous clarity & passion his approach to team coaching, the power of this methodology and the gift it can afford teams and organisations especially in answer to our VUCA world. Alexander describes the company's approach to team interventions, the models he deploys and the role of the team coach. He shares many vignettes to illuminate the power of team coaching and how by way of this work the teams work is sustainable. Noteworthy points of discussion Alexander Caillet saw the power of Group Dynamics in his studies at Columbia University and he wanted to bring this power into his work with teams. Team Coaching is the product of integrating a real time approach in group dynamics in a business or organisational setting with the team is doing its work so that they can adapt and get at improved performance Teams and groups today are brought into the work using tools to support the building of team charters, defining a purpose, clarifying goals understanding roles etc. but they stumble on communication, trust and respect and although we have tools for these ideas they are seemingly insufficient when the team is back in the workplace. The real translation of those tools around communication trust, psychological safety happens in the moment when the team has a breakdown back at work and they can see how to navigate these issues with real time coaching. There is almost an implicit assumption at work on teams that “we don't do that kind of work it is too dangerous” but group dynamics in terms of norms/patterns/outcome are unavoidable and without it we lose opportunity to get a HPTs. There is a role with teams for Team Facilitation, Team Building, Team Teaching and Consulting and there is also room for Team Coaching. Corentus offers a customised approach that is a combination of all four modalities Team Coaching is an engagement over time sometime a year-that allows for team observation and analysis of its processes and patterns to further sustained difference Corentus employs a definitive model to support teams engage with their work: The model is described as Sensing/Being/Making moves. Being describes the way a Coach shows up-important to be fit for the work as this work is not for the faint of heart-essential to be grounded and to understand triggers – being calm and clear gets noticed and helps the team Sensing is about gathering data either quantitatively or qualitatively and then feeding it back to the team in a neutral manner . Corentus uses many tools but important to them is the idea of the relationship between norms/patterns and outcomes or the OPN model – all teams have patterns laid down by norms, assumptions and beliefs that generate definitive outcomes Making Moves is about using the data in the various domains of decision making/accountability etc for difference Having data and asking powerful questions in the moment when the team is doing its work can often be a real turning point for a team and the real value of Team Coaching We walk into systems very quickly and often we become blind or go native to the system we are in. Team Coaching allows us to pause and notice and ask the team to do the same- to notice what is happening, what patterns are emerging and what needs to be changed if anything? Important to this work is Leadership Coaching in addition to working with the Leader and team publicly, Corentus will work with the Leader and their impact offline over time. We are living in a VUCA world Volatility represents the relentless speed & pace of change, Uncertainty meaning things are harder to predict, Complexity making things difficult to analyse and Ambiguity making things harder to interpret. VUCA drives a lot of overwhelm. Corentus and Alexander sees a real need for teams to focus, for teams to invest time together, to come together to work things out. Alexander sees the work of Team Coaches as instrumental and of value in this VUCA world if we can transfer the knowledge and skills we use with teams so that they can sense/be and make moves for themselves. Alexander shared a powerful story of work with a with a well-known NGO and how his approach and models supported the Executive Team of this entity ultimately make decision four times faster than they ordinarily were able to do. This ability and skill learnt over time was of enormous value when in reality the NGO was faced with a humanitarian crisis after the Tsunami in 2008. Alexander is intent on building Corentus Globally , writing and speaking more, stepping out of delivery and having an impact in terms of increased global collaboration and collective intelligence . I wish him all the success in these endeavours Resources: the following include the resources we alluded to over the course of our conversation WWW.corentus.com Introduction to Corentus Team Coaching by Alexander Caillet and Amy Yeager
Do decembra 2021 so vse lokalne skupnosti v Sloveniji dolžne vizijo svojega prostorskega razvoja opredeliti z občinskim prostorskim načrtom (OPN). V Mestni občini Koper se je nabralo že več kot sedem tisoč pobud občanov. Ena od iniciativ za razvoj prostora je Automatik delovišče, skupina 20 akterjev z različnih področij, ki si prizadevajo za med seboj povezan razvoj mesta in ustanovitev neodvisnega urbanističnega sveta. Deloval bi kot mediator v povezavi z županom, mestnim urbanistom in javnostjo. Foto: Miran Kambič
Renowned entrepreneur and bestselling author of the Leadsology series, Tom Poland, shares a simple way he's partnered with influencers to generate a continuous flow of highly qualified leads for his consulting business. Tom Poland's Lessons to Partner with Influencers During the conversation, Tom reveals: The power of "OPN" to share your message in a grander way and land new clients The four R's to connect with business partners The system to create a constant stream of client consultations on your calendar The Prius factor to build connections with influences What to do when you feel you should apply a certain marketing strategy that everyone else seems to be applying The reason he doesn't favor common marketing strategies such as Facebook ads and blogging, and what really works instead to get new clients The two key streams of leads for his business, and how careful documentation of each step in the process and a team of contractors allowed him to create a seamless process to grow his business About Tom Poland Tom Poland is the best-selling author of the Leadsology® series and has started and sold numerous businesses with multi-million dollar revenues. He tells me that he is voluntarily married to a pretty German Frau who he affectionately refers to as his Little Bavarian Bulldozer. They live quietly and happily on the sand next to the waves at little Castaways Beach in Queensland, Australia. Learn more about Tom at https://www.leadsology.guru/ Check out Tom's new book: Leadsology: Marketing the Invisible Take Tom's Five Hour Lead Generation Challenge www.fivehourchallenge.com Join Tom's Facebook group: www.facebook.com/groups/leadsology/
Nutanix er den mest fleksible og simple HCI-løsning på markedet. Opnå en infrastruktur hvor du kan slå features til og fra, som du ønsker. Administrationen af platformen kan sammenlignes cloud administration - få fx mikrosegmenteret dit netværk med ét klik. I denne episode af Arrow Pitch får du et overblik over Nutanix-platformen.
Nutanix er den mest fleksible og simple HCI-løsning på markedet. Opnå en infrastruktur hvor du kan slå features til og fra, som du ønsker. Administrationen af platformen kan sammenlignes cloud administration - få fx mikrosegmenteret dit netværk med ét klik. I denne episode af Arrow Pitch får du et overblik over Nutanix-platformen.
Nutanix er den mest fleksible og simple HCI-løsning på markedet. Opnå en infrastruktur hvor du kan slå features til og fra, som du ønsker. Administrationen af platformen kan sammenlignes cloud administration - få fx mikrosegmenteret dit netværk med ét klik. I denne episode af Arrow Pitch får du et overblik over Nutanix-platformen.
After the last few month’s episodes on wine, we’re cracking back into cocktails – specifically how the French drink them at home and new technologies designed to help with your DIY drinks. To unpack all that, we invited Philippe-Alexandre Bernatchez of Opn into the studio. Philippe-Alexandre is the Senior Marketing Project Manager at Opn. Born […]
Data er din konkurrencefordel. Opnå en forbedret brug af data gennem bedre datakvalitet, kortere cyklustid og overlegen datahåndtering med brug af Hitachi Vantara DataOps. Hitachi Vantara fremmer innovationen og styrker konkurrenceevnen mellem mennesker, processer og teknologi og gør dig dermed i stand til fuldt ud at styre, berige og transformere og levere indsigt i hele din virksomhed. Muligheder som kan øge din kundeforståelse, kundetilfredshed, forretningsoptimere og give dig et bedre grundlag for at træffe bæredygtige beslutninger i fremtiden.
Data er din konkurrencefordel. Opnå en forbedret brug af data gennem bedre datakvalitet, kortere cyklustid og overlegen datahåndtering med brug af Hitachi Vantara DataOps. Hitachi Vantara fremmer innovationen og styrker konkurrenceevnen mellem mennesker, processer og teknologi og gør dig dermed i stand til fuldt ud at styre, berige og transformere og levere indsigt i hele din virksomhed. Muligheder som kan øge din kundeforståelse, kundetilfredshed, forretningsoptimere og give dig et bedre grundlag for at træffe bæredygtige beslutninger i fremtiden.
Come ha fatto Instagram a diventare il colosso che è oggi?Ha usato il Growth Hacking, come molte aziende dell'ultimo decennio, e tra le diverse tecniche utilizzata ce n'è una che voglio analizzare in questo video.Si chiama OPN, si basa su un concetto semplice, ma potente.Strategie di questo tipo possono essere utilizzate da qualsiasi business per trovare clienti o trovare utenti, indipendentemente dal settore e dalla grandezza.Come trovare nuovi clienti è forse uno degli ostacoli più grossi che affrontano le aziende (soprattutto PMI e Startup) e questi concetti potrebbero essere utili ad acquisire clienti con costi contenuti.
Renowned entrepreneur and bestselling author of the Leadsology series, Tom Poland, shares the strategy that has allowed him to collaborate with influencers to generate a continuous flow of highly qualified leads for his consulting business. Tom Poland's Lessons to Grow Your Business by Collaborating with Influencers During the conversation, Tom reveals: The power of "OPN" to share your message in a grander way and land new clients The four R's to connect with business partners The system to create a constant stream of client consultations on your calendar The Prius factor to build connections with influences What to do when you feel you should apply a certain marketing strategy that everyone else seems to be applying The reason he doesn't favor common marketing strategies such as Facebook ads and blogging, and what really works instead to get new clients The two key streams of leads for his business, and how careful documentation of each step in the process and a team of contractors have allowed him to create a seamless process to grow his business About Tom Poland Tom Poland is the best-selling author of the Leadsology® series and has started and sold numerous businesses with multi-million dollar revenues. He tells me that he is voluntarily married to a pretty German Frau who he affectionately refers to as his Little Bavarian Bulldozer. They live quietly and happily on the sand next to the waves at little Castaways Beach in Queensland, Australia. Learn more about Tom at https://www.leadsology.guru/ Check out Tom's new book: Leadsology: Marketing the Invisible Take Tom's Five Hour Lead Generation Challenge www.fivehourchallenge.com Join Tom's Facebook group: www.facebook.com/groups/leadsology/
Propelling organic produce from fringe to mainstream. In This Podcast: The story of how organic produce and food became mainstream in our grocery stores is shared by Tonya Antle who was actively driving this change before most of us even knew we needed it. She found her passion in the early stages of the organic movement and helped propel it across the nation. Now there is a new focus with the Organic Grower Summit happening in December, and she shares some very useful tips as a highlight of what will be available then. Don't miss an episode! Click here to sign up for podcast updatesor visit www.urbanfarm.org/podcast Tonya grew up on her family's table grape and citrus farm in Delano, California. From being a vice president of organic sales to consulting about organic ag investments, she has been a driving force in organic farming for more than 30 years, and has received several awards recognizing her work in the ag industry. Currently, she is an adjunct professor of Fresh Fruit and Vegetable Marketing at Cal Poly, San Luis Obispo, a guest lecturer at both Hartnell Junior College and California State University Monterey Bay, and she is the Co-founder and Executive Vice President of the Organic Produce Network (OPN), which launched in January 2017. Go to www.urbanfarm.org/opn for more information and links on this podcast, and to find our other great guests.
The Building Science Podcast got a couple of Press Passes and went to New York City last week for the AIA Conference on Architecture, 2018. What an incredible conference it was! We had the opportunity to connect with so many thoughtful and visionary architects who want to build a better, healthier future. We're psyched. One of the most thoughtful conversations we had was with Corey Squire and Tate Walker about the new Committee On The Environment's new Toolkit. It's a resource-rich document that helps firms and projects of any kind measure their progress against benchmarks of sustainability without restrictive prescription pathways, while keeping outcomes at the central focus.COREY SQUIRELEED AP O+MSustainability Process Manager, Lake|Flato ArchitectsCorey works with all Lake|Flato teams to establish sustainability goals, analyze designs with simulation software, and collects post-occupancy performance data. He received a Bachelor of Arts in Environmental Studies from Oberlin College and a Master of Architecture from Tulane University. In 2012, Squire was awarded the Eskew+Dumez+Ripple Research Fellowship to study building post-occupancy energy performance and sustainable design processes.Tate WalkerAIA, LEEP AP BD+C, Sustainability Director, OPN ArchitectsAs an architect focused on energy and sustainability in the built environment, he steers OPN’s sustainability initiatives, focusing on energy research, technology evaluation, and high performance design. He regularly writes and presents on issues relating to energy, technology, building science, and promoting environmental awareness through design. His experience includes integrating teams, design charrettes, building systems, and sustainability initiatives on capital projects.Tate has worked nationally for clients such as Northwestern University, National Renewable Energy Laboratory (NREL) and Iowa State University. He served the United States Green Building Council in various capacities since 2008 and has been a leader within the Wisconsin Green Building Alliance since 2005, including serving as the organization’s vice president in 2009.At OPN, Tate led the adoption and administration of the 2030 Challenge to transition to net zero energy buildings. He also is chair of the firm’s annual Green Day event and an internal sustainability committee.He is currently leading the sustainable design for the new Advanced Teaching and Research Building for the Biosciences at Iowa State University, which is targeting LEED Gold. The 115,000 square foot, $52 million project will be an anchor building on campus, incorporating innovative site, water, advanced materials and daylighting components to support a unique, sustainable experience for its users.About The Committee On The EnvironmentCOTE MissionThe Committee on the Environment (COTE) works to advance, disseminate, and advocate—to the profession, the building industry, the academy, and the public—design practices that integrate built and natural systems and enhance both the design quality and environmental performance of the built environment. COTE serves as the community and voice on behalf of AIA architects regarding sustainable design and building science and performance.COTE reflects the profession’s commitment to provide healthy and safe environments for people and is dedicated to preserving the earth’s capability of sustaining a shared high quality of life. The committee’s mission is to lead and coordinate the profession’s involvement in environmental and energy-related issues and to promote the role of the architect as a leader in preserving and protecting the planet and its living systems.COTE provides the AIA with knowledge about environmental issues and advises the Institute on environmental policy matters affecting the practice of architecture. The committee supports cooperation with educators and institutions of learning, manufacturers, government agencies, environmental organizations, and industry groups in advancing environmentally sound design processes and standards as well as environmentally innovative materials and integrated systems.COTE Goals-To advance the importance of sustainable design to our fellow architects, within the Institute, and to the broader public.-To educate architects about the environmental and energy-related impacts of design decisions & about how to incorporate sustainable design into daily practice.-To define and promote the cutting edge of sustainable design for our profession.-To foster leadership among architects in all facets of environmental decision making.-To recognize environmental leadership of architects in practice, education, industry, and government.-To influence the direction of architectural education to place more emphasis on ecological literacy , sustainable design and building science-To maintain, refine, and strengthen alliances with professional and trade associations and other leaders in environmentally responsible design to coordinate our sustainable agendas to make our message stronger.-To maintain, refine, and strengthen alliances with other AIA Knowledge Communities and committees, and serve as a resource to Institute initiatives and projects that promote sustainability in the built environment.-To green AIA convention venues and meetings.-To communicate the AIA’s environmental and energy-related concerns to the public and private sectors and influence the decisions of the public, professionals, clients, and public officials on the impact of their environmental and energy-related decisions.-To educate architects on regulatory, performance, technical and building science issues and how those issues influence architecture. Educate the architectural profession on programming, designing, and managing building performance.-To investigate and disseminate information regarding building performance best practices, criteria, measurement methods, planning tools, occupant-comfort, heat/air/moisture interfaces between the interior and exterior of buildings.-To promote a more integrated practice in order to achieve environmentally and economically efficient buildings. One of the tools we will plan to promote to achieve this integration is Building Information Technology (BIM).COTE Toolkit Please check out this resource rich document. It's absolutely incredible and this is only Version 1! More great improvements to come. If you're not an AIA member and can't access it, write us and we'll see what we can do about getting you a copy. The more people who can work with the tools provide here, the better outcomes our projects will be. podcast@positiveenergy.pro"Why We Let Ourselves Do Mediocre Work"Great article from Building Green Magazinethat dispels the myth of the "unicorn client" that will come around and make all your wildest dreams come true to design and build sustainably.The Shape Of GreenDoes going green change the face of design or only its content? The first book to outline principles for the aesthetics of sustainable design, The Shape of Green argues that beauty is inherent to sustainability, for how things look and feel is as important as how they’re made.In addition to examining what makes something attractive or emotionally pleasing, Hosey connects these questions with practical design challenges. Can the shape of a car make it more aerodynamic and more attractive at the same time? Could buildings be constructed of porous materials that simultaneously clean the air and soothe the skin? Can cities become verdant, productive landscapes instead of wastelands of concrete?Drawing from a wealth of scientific research, Hosey demonstrates that form and image can enhance conservation, comfort, and community at every scale of design, from products to buildings to cities. Fully embracing the principles of ecology could revolutionize every aspect of design, in substance and in style. Aesthetic attraction isn’t a superficial concern — it’s an environmental imperative. Beauty could save the planet.The Last Auto MechanicGreat blog post by Tom Price (renewable energy entrepreneur. A-EV cheerleader. Founder Black Rock Solar. Recovering journalist, middling mountain biker. Formerly of Capitol Hill, SLC, & BRC) about the ways America’s transportation economy and landscape is about to be utterly transformed into a world beyond driving. Or drivers. Or even car mechanics. Enjoy the ride.Game of ThronesNew Yorker article about the intricate design of seating on commercial flights and the impacts it has on travelers.Habits of High Performing FirmsAIA Committee on the Environment (COTE) released a report called “The Habits of High-Performance Firms” which follows up on the previous “Lessons from the Leading Edge," which is a comprehensive study of two decades of AIA COTE Top Ten Award winners. Launched in 1997, the annual awards are the profession’s longest-running and “best known recognition program for sustainable design excellence,” according to the AIA.Biophilic Design Biophilic Design is an innovative way of designing the places where we live, work, and learn. We need nature in a deep and fundamental fashion, but we have often designed our cities and suburbs in ways that both degrade the environment and alienate us from nature. The recent trend in green architecture has decreased the environmental impact of the built environment, but it has accomplished little in the way of reconnecting us to the natural world, the missing piece in the puzzle of sustainable development. Come on a journey from our evolutionary past and the origins of architecture to the world’s most celebrated buildings in a search for the architecture of life. Together, we will encounter buildings that connect people and nature - hospitals where patients heal faster, schools where children’s test scores are higher, offices where workers are more productive, and communities where people know more of their neighbors and families thrive. Biophilic Design points the way toward creating healthy and productive habitats for modern humans.Architecture 2030Edward Mazria, FAIA, Hon. FRAICFounder and CEOEdward Mazria is an internationally recognized architect, author, researcher, and educator. Over the past decade, his seminal research into the sustainability, resilience, energy consumption, and greenhouse gas emissions of the built environment has redefined the role of architecture, planning, design, and building, in reshaping our world. He is the founder of Architecture 2030, a think tank developing real-world solutions for 21st century problems, and host of the AIA+2030 Professional Education Series and 2030 Districts movement in North American cities.Mazria issued the 2030 Challenge and introduced the 2030 Palette, a revolutionary new platform that puts the principles behind low-carbon/zero carbon and resilient built environments at the fingertips of architects, planners, and designers worldwide. In 2014 he presented the Roadmap to Zero Emissions at the Organization of Economic Cooperation and Development and UN Framework Convention on Climate Change calling for zero emissions in the built environment by 2050, and drafted the 2050 Imperative, endorsed by professional organizations representing over 1.3 million architects in 124 countries worldwide. In 2015 he launched the China Accord, which has been adopted by key international firms pledging to plan, design and build to carbon neutral standards in China; and delivered the opening presentation at the UNFCCC COP21 “Buildings Day” titled The 2 Degree Path for the Building Sector.Recently, he developed Achieving Zero, a framework of incremental actions that cities and governments can put in place to ensure carbon neutral built environments by mid-century, and the Zero Cities Project (with the Carbon Neutral Cities Alliance, Urban Sustainability Directors Network, New Buildings Institute, and Resource Media) to implement the framework.Mazria speaks nationally and internationally on the subject of architecture, design, energy, economics, and climate change and has taught at several universities, including the University of New Mexico, University of Oregon, UCLA, and the University of Colorado-Denver.Mr. Mazria’s awards include AIA Design Awards, American Planning Association Award, Department of Energy Awards, American Solar Energy Society Pioneer Award, Equinox Award, National Conservation Achievement Award, Mumford Award from Architects/Designers/Planners for Social Responsibility, inaugural Hanley Award, Distinguished Career Award from Pratt Institute, Zia Award from the University of New Mexico, Game Changers Award from Metropolis Magazine, 2011 Purpose Prize, and the 2015 Kemper Award from the American Institute of Architects. He is a senior fellow of the Design Futures Council, Honorary Fellow of the RAIC, and received an Honorary Doctor of Architecture degree from Illinois Institute of Technology.COTE Toolkit ContributorsTate Walker, AIA, Project Co-Lead OPN Architects, Madison, Wisconsin, COTE Advisory GroupCorey Squire, AIA, Project Co-Lead Lake|Flato Architects, San Antonio, Texas, COTE Advisory GroupAnne Hicks Harney, FAIA, Long Green Specs, Baltimore, MarylandBetsy del Monte, FAIA, Cameron MacAllister Group, Dallas, TexasDavid Hincher, AIA, Kieran Timberlake, Philadelphia, PennsylvaniaGunnar Hubbard, FAIA, Thornton Tomasetti, Portland, Maine, COTE Advisory GroupHelena Zambrano, AIA, Overland Partners, San Antonio, TexasMary Ann Lazarus, FAIA, Cameron MacAllister Group, St. Louis, Missouri, COTE Advisory GroupStephanie Horowitz, AIA, ZeroEnergy Design, Boston, Massachusetts, COTE Advisory GroupStephen Endy, AIA, Mahlum, Portland, OregonVikram Sami, AIA, Olson Kundig, Seattle, Washington, COTE Advisory GroupZ Smith, FAIA, Eskew+Dumez+Ripple, New Orleans, Louisana
Why do we hear the term 'back to basics' so frequently? Because it works! Regardless of what you are doing, going 'back to basics' is something everyone understands. Being "brilliant at the basics" is where diamonds are found! In this week’s episode, Stefan Kazakis delivers a key lesson in how to develop a competitive advantage in your business. First and foremost, it is about attitude. To be able to create a competitive advantage is not about having the cheapest price, or working harder. It’s about being effective and efficient. That’s where the money is. It’s about having the right business education and understanding to focus on the right things in your business to build better leverage. Stefan explains the three diamonds needed to create leverage - OPT, OPM and OPN. Other people’s time, other people’s money and other people’s networks. All this and more in this week’s podcast. ASK STEFAN: Do you have a question for Stefan to answer in an upcoming episode? Submit your questions in the comments, via email to stefan@businessbenchmarkgroup.com.au or get in touch with us on social media!
Comme à chaque début de mois, l'équipe de Pôle Hip-Hop passe au crible les sorties marquantes du mois qui précède. On note entre autres les performances remarquables d'Enima, High Klassified et Jai Nitai Lotus. On apprécie les projets de Cardi B et Princess Noka. Et Myth Syzer, TripleGo, Alkpote et Médine nous livrent également des albums intéressants, avec leurs forces et leurs faiblesses. On écoute des morceaux de Royce da 5'9", Enima, High Klassified, The Internet, Medine, Myth Syzer et TripleGo. Horaire 4:40 - Jingle 5:36 - Mot d'ouverture 8:20 - Enima - Saisons 11:21 - High Klassified - 1919 (Feat. Zach Zoya) 14:30 - Retour sur les sorties rap québ 15:30 - Enima - OPN 20:19 - Fouki - Zay 24:10 - High Klassified - Kanvaz EP 26:45 - PRO-V - PRO Knows... The Opus 27:50 - Nicholas Craven - Conway Remixes 29:10 - Jai Nitai Lotus - Affirmations 34:32 - TripleGo - Medellin 38:10 - Myth Syzer - Sans toi (Feat. Hamza) 41:50 - Retour sur les sorties rap français 42:10 - TripleGo - #EnattendantMachakil 45:24 - Myth Syzer - Bisous 51:10 - Alkpote - Inferno 55:00 - Médine - Storyteller 57:57 - 13 Block - Triple S, Dinos - Imany, Veerus - Iceberg Slim 62:20 - Myth Syzer - Austin Power 65:35 - Médine - Enfant du destin : Ataï 70:00 - Retour sur les sorties rap US 70:00 - Cardi B - Invasion of Privacy 72:20 - Princess Nokia - A Girl Cried Red 76:20 - J. Cole - KOD 79:10 - Saba - Care For Me, Robert Glasper & Kaytranada - The ArtScience Remixes, Nas - Illmatic: Live From The Kennedy Center, Lil Xan - Total Xanarchy 85:45 - Royce Da 5'9" - Dumb 88:52 - The Internet - Roll (Burbank Funk)
A podcast with music by developer and publisher Micro Cabin, best known for the Xak series and its various spin-offs. Many of their early releases originated on Japanese computers and featured an excellent line-up of composers like Tadahiro Nitta, Ryuji Sasai, Yasufumi Fukuda, and Yukiharu Urita who created some spectacular soundtracks, especially for the 'lesser' FM chips like OPLL and OPN. Continue reading →
Why is the transparency agenda critical to rebuilding citizens’ trust in our governance institutions? How have international movements been working to promote a new form of relationship between citizens and their governments? And how do Parliaments fit into this agenda? Citizen trust in democratic institutions, including parliaments, is critical to the functioning of democracy and effectiveness of governance. As the institution that represents citizens and translates their interests to legislation, parliaments are particularly affected by declining trust and blurred perceptions about their core functions. As the institution that is closest to citizens, it is therefore not surprising that parliaments are emerging at the center of international platforms and national efforts to retain and restore public confidence in democratic institutions by, for example, adopting measures to provide information on their day-to-day activities in more accessible and transparent ways. The Open Government Partnership (OGP) is an example of a multilateral initiative that aims to restore public confidence by securing concrete commitments from governments to promote transparency, fight corruption, empower citizens, and harness new technologies to strengthen democratic governance. The OGP currently has 75 participating countries including Canada, which have collectively made over 2,500 related commitments. The transparency agenda – and its pursuit through platforms like the OGP – are not exclusive to the executive branch of government. Parliaments in many countries – particularly those in the Americas – have been driving this agenda by working with citizens to develop their own internal action plans, and by functioning as models for transparency in their country. Increasing numbers of parliaments are also developing standalone chapters as part of their country’s OGP commitments. Through leadership roles in the OGP Legislative Openness Working Group and the ParlAmericas Open Parliament Network (OPN) Senator Hernán Larraín has been instrumental in positioning parliaments at the centre of this transparency agenda. In his address, he will consider progress achieved and upcoming opportunities, like the next Summit of the Americas about Democratic Governance against Corruption in April 2018 in Peru, to incorporate a parliamentary perspective and a focus on citizen trust in the shaping of the transparency agenda. This intervention was recorded during the ParlAmericas Canadian Section Annual Parliament Hill reception where Senator Larraín was invited to be the Guest Speaker to discuss how a number of parliaments in the Americas and the Caribbean are leading by example within the Open Government Partnership to be at the cutting edge of the transparency agenda.
Tonya Antle has been a driving force in the farming and produce industry for the past thirty plus years. She grew up on her own family’s table grape and citrus farm in Delano, California. She is recognized as a top spokesperson and a true trailblazer for the organic produce segment, helping to propel organic produce from the fringe to mainstream mass-market retailing. Tonya is the Cofounder and EVP of the newly formed Organic Produce Network. OPN, based in Salinas, Ca, is focused on connecting all facets of the organic produce, specialty crop community through education, information and live events. She previously held the position of Vice President of Organic Sales for over 13 years at Earthbound Farm, the country’s leading brand of organic vegetables and salads. During her tenure at Earthbound Farm, she worked with leading retailers throughout the US and Canada to develop and build organic programs and markets. Tonya was an instrumental leader in helping to build this California brand to its half billion-dollar sales level prior to her departure.Tonya brings an unabashedly positive outlook and can-do attitude to everything she does and understands the power of the judicious use of humor, even in business, which means that she’s in high demand. She has also consulted for leading US Venture Capital firms on investments in the organic space. Tonya, is the 2017 recipient of the Ag Against Hunger, Ag Women of the Year, the 2016 co-recipient, along with her husband, Rick, of the United Fresh, Lifetime Achievement Award, and the 2003 recipient of the Women in Produce achievement award. In 2010, she joined the Ag Business Department at Cal Poly, San Luis Obispo as an Adjunct Professor in Fresh Fruit and Vegetable marketing and has also served the university as a member of the President’s Council. Tonya also enjoys guest lecturing at Hartnell Junior College, Ag Business Department and most recently at CSUMB, School of Business. She currently leads the Women's Fund Leadership Council which is a field of interest fund at the Community Foundation for Monterey County, where she recently completed a 1.5-million-dollar endowment campaign for this fund. Tonya also serves on the CSUMB Foundation Board. Tonya holds a Bachelors of Arts degree in Communications from the University of California at Irvine. Her time is well balanced between her entrepreneurial role in the organic produce industry, community volunteerism, mother, and wife. Tonya takes great pleasure in the time she spends with her husband Rick, CEO of Tanimura and Antle, whether it’s boating the waterways of the Americas or playing tennis in Pebble Beach. Tonya brings a joyful enthusiasm to all of her endeavors.
I hung out with Warp Records electronic, experimental, ambient, drone producer Daniel Lopatin aka Oneohtrix Point Never (or sometimes OPN) in an unusually busy alleyway behind Vancouver's Venue nightclub. He sipped tea the whole time to massage his vocal chords for that night's show. His latest record, Garden of Delete, started when Daniel got really obsessed with top 40 music and wondered what it would be like to create an entire album from the discarded song ideas of top 40 pop songwriters. The record ended up being about an adolescent alien meeting a made-up hyper-grunge band called Chaos Edge and their musical collaboration. Which song on his record is about how absurd it is to ejaculate for the first time? How did top 40 song writers react when Daniel approached them for their discarded song ideas? Which is his favorite track on Garden of Delete? Which top 40 song is Daniel's current guilty obsession? Which Garden of Delete track does Daniel call his "psychotic clown" song? In how many different ways can our interview get interrupted by this abnormally busy alleyway? You'll have to listen to find out. Tracks played on the the show… 1) Oneohtrix Point Never, Garden of Delete (2015) “Sticky Drama” 2) Rae Sremmurd, SremmLife (2015) “No Type” 3) Oneohtrix Point Never, Garden of Delete (2015) “Mutant Standard” 4) Oneohtrix Point Never, Garden of Delete (2015) “Animals” 5) Oneohtrix Point Never, Replica (2011) “Replica” 6) Oneohtrix Point Never, Garden of Delete (2015) “I Bite Through It” NO songs CANCON
I hung out with Warp Records electronic, experimental, ambient, drone producer Daniel Lopatin aka Oneohtrix Point Never (or sometimes OPN) in an unusually busy alleyway behind Vancouver's Venue nightclub. He sipped tea the whole time to massage his vocal chords for that night's show. His latest record, Garden of Delete, started when Daniel got really obsessed with top 40 music and wondered what it would be like to create an entire album from the discarded song ideas of top 40 pop songwriters. The record ended up being about an adolescent alien meeting a made-up hyper-grunge band called Chaos Edge and their music collaboration. Which song on his record is about how absurd it is to ejaculate for the first time? How did top 40 song writers react when Daniel approached them for their discarded song ideas? Which is his favorite track on Garden of Delete? Which top 40 song is Daniel's current guilty obsession? Which Garden of Delete track does Daniel call his "psychotic clown" song? In how many different ways can our interview get interrupted by this abnormally busy alleyway? You'll have to listen to find out. Tracks played on the the show… 1) Oneohtrix Point Never, Garden of Delete (2015) “Sticky Drama” 2) Rae Sremmurd, SremmLife (2015) “No Type” 3) Oneohtrix Point Never, Garden of Delete (2015) “Mutant Standard” 4) Oneohtrix Point Never, Garden of Delete (2015) “Animals” 5) Oneohtrix Point Never, Replica (2011) “Replica” 6) Oneohtrix Point Never, Garden of Delete (2015) “I Bite Through It” NO songs CANCON
9-23 4P 4P Berra RIP, birthday song, OPN, TV talk
Osteopontin represents a multifunctional molecule playing a pivotal role in chronic inflammatory and autoimmune diseases. Its expression is increased in inflammatory bowel disease (IBD). The aim of our study was to analyze the association of osteopontin (OPN/SPP1) gene variants in a large cohort of IBD patients. Genomic DNA from 2819 Caucasian individuals (n = 841 patients with Crohn's disease (CD), n = 473 patients with ulcerative colitis (UC), and n = 1505 healthy unrelated controls) was analyzed for nine OPN SNPs (rs2728127, rs2853744, rs11730582, rs11739060, rs28357094, rs4754 = p.Asp80Asp, rs1126616 = p.Ala236Ala, rs1126772 and rs9138). Considering the important role of osteopontin in Th17-mediated diseases, we performed analysis for epistasis with IBD-associated IL23R variants and analyzed serum levels of the Th17 cytokine IL-22. For four OPN SNPs (rs4754, rs1126616, rs1126772 and rs9138), we observed significantly different distributions between male and female CD patients. rs4754 was protective in male CD patients (p = 0.0004, OR = 0.69). None of the other investigated OPN SNPs was associated with CD or UC susceptibility. However, several OPN haplotypes showed significant associations with CD susceptibility. The strongest association was found for a haplotype consisting of the 8 OPN SNPs rs2728127-rs2853744-rs11730582-rs11439060-rs28357094-rs112661-rs1126772-rs9138 (omnibus p-value = 2.07×10⁻⁸). Overall, the mean IL-22 secretion in the combined group of OPN minor allele carriers with CD was significantly lower than that of CD patients with OPN wildtype alleles (p = 3.66×10⁻⁵). There was evidence for weak epistasis between the OPN SNP rs28357094 with the IL23R SNP rs10489629 (p = 4.18×10⁻²) and between OPN SNP rs1126616 and IL23R SNP rs2201841 (p = 4.18×10⁻²) but none of these associations remained significant after Bonferroni correction. Our study identified OPN haplotypes as modifiers of CD susceptibility, while the combined effects of certain OPN variants may modulate IL-22 secretion.
Medizinische Fakultät - Digitale Hochschulschriften der LMU - Teil 12/19
Osteogenic differentiation of hMSC into osteoblasts is a prerequisite for subsequent bone formation. Numerous studies have explored osteogenic differentiation under standard tissue culture conditions, which usually employ 21% of oxygen. However, bone precursor cells such as hMSC reside in stem cell niches of low oxygen atmospheres. Furthermore, they are subjected to low oxygen concentrations when cultured on three dimensional scaffolds in vitro for bone tissue engineering purposes, and even more so after transplantation when vascularisation has yet to be established. Similarly, hMSC are exposed to low oxygen in the fracture microenvironment following bony injury. Recent studies revealed that hypoxic preconditioning improves cellular engraftment and survival in low oxygen atmospheres. In the present study we therefore investigated the osteogenic differentiation potential of hMSC under 2% O2 (hypoxia) in comparison to a standard tissue culture oxygen atmosphere of 21% (normoxia). The success of differentiation was validated through Alizarin red staining and RT-PCR analysis of osteoblast markers ALP and OPN. We assessed osteogenic differentiation of hMSC following hypoxic preconditioning to address whether this pretreatment is beneficial for subsequent differentiation under low oxygen tension. To validate our findings we carefully characterised the extent of hypoxia exerted on cells with respect to cell survival (WST assay) and proliferation (growth curve). Furthermore we also tried to elucidate the role of HIF-1 alpha with respect to osteogenic differentiation under hypoxia via silencing RNA and DFO, a pharmacological agent. Finally we tested whether an immortalized hMSC-line (SCP-1) would serve as a model system for hMSC. We found that hMSC proliferate better if cultured under 2% of oxygen. We confirmed that osteogenic differentiation of hMSC is indeed inhibited under hypoxia. We showed for the first time that hypoxic preconditioning of hMSC prior to osteogenic induction restores osteogenic differentiation of hMSC under hypoxia. HIF-1 alpha seemed not to play a significant role in osteogenic differentiation under hypoxia, as transiently knocking down of HIF-1 alpha in preconditioned samples did not show any differences in their osteogenic differentiation. Moreover stabilising Hif-1 alpha in hypoxic samples did not yield any osteogenic differentiation either substantiating the notion that HIF-1 alpha does not have a direct role in the osteogenic differentiation of hMSC under hypoxia. Together our data suggest that hypoxia favours stemness over differentiation by upregulating embryonic stem cell markers like OCT-4 and NANOG. Hypoxic preconditioning may help to restore the otherwise reduced osteogenic potential of hMSC, either within a hypoxic fracture environment or at the site of implantation of tissue engineered bone constructs. We therefore believe that hypoxic preconditioning is a helpful tool for successful regenerative cell-based therapies in bone tissue engineering. SCP-1 cells might be used as a model system for hMSC as they are easy to handle, can be cultured to a desired cell number within a very short period of time, are relatively inexpensive and above all do not go into senescence as seen with hMSC after approximately 20 passages. Apart from their distinct advantages SCP-1 cells still maintain the specific CD markers characteristic for hMSC and are able to differentiate into adipogenic, osteogenic and chondrogenic lineages. However for in vivo experiments in animals a constant monitoring of neoplastic transformation is mandatory
Background: Evaluation of the protein osteopontin (OPN) as a potential new marker in comparison to melanoma inhibitory activity (MIA) for screening and detection of metastatic uveal melanoma. Methods: Plasma levels of 32 patients with uveal melanoma were analyzed for OPN and MIA by enzyme-linked immunosorbent assay (ELISA). Fourteen of these patients had clinically detectable liver metastases. Results: Median plasma concentration of OPN in patients with metastatic disease was 152.01 ng/ml compared to 47.39 ng/ml in patients without clinically detectable metastases (p < 0.001). The difference between the median MIA plasma levels in patients with (13.11 ng/ml) and patients without (5.64 ng/ml) metastatic disease was also statistically significant (p < 0.001). No correlation could be found between MIA or OPN levels and tumor height in patients without clinically detectable metastases. Conclusion: The proteins MIA and OPN seem to be promising tumor markers for the metastasis screening in patients with uveal melanoma. Copyright (C) 2009 S. Karger AG, Basel
© 2020-2025 Ivy Podcast Discovery LLC
