Podcasts about vhh

Bill Eldridge is an experienced scientific and commercial leader, one of the founding team members of the VHH antibody company Isogenica, and now their CEO. Bill joined us to talk about his career, the huge potential that still exists within antibody discovery and the magic of seeing a 96 well ELISA plate turn blue after months of failure! Enjoy...

This episode features an interview with Amir Reichman, CEO of Scinai Immunotherapeutics, a biopharma company focused on developing, manufacturing and commercializing cutting-edge biological treatments within the inflammation and immunology (I&I) sector, with a primary focus on addressing autoimmune and infectious diseases.Scinai Immunotherapeutics is working on developing nanosized antibodies from alpacas, known as VHH-antibodies or NanoAbs, which show promise as improved biological therapies for various illnesses. These NanoAbs are being tailored to treat conditions with significant unmet medical requirements and promising market prospects, including psoriasis and asthma.Prior to being appointed as Scinai's CEO in 2021, Mr. Reichman recently held the position of Head of Global Vaccines Engineering Core Technologies and Asset Management at the GSK Vaccines headquarters in Belgium. Before that, he was the Senior Director of the Global GSK Vaccines Supply Chain. He became part of GSK in 2015 following its acquisition of Novartis Vaccines, where he had various leadership positions with growing responsibilities within the Global Vaccines Supply Chain Management team, based in Holly Springs, NC, USA.Tune into the episode to hear more about the development of new, cutting-edge inflammation and immunology therapies. Mr. Reichman also shares insights into the most significant trends currently shaping the inflammation and immunology sector.For more life science and medical device content, visit the Xtalks Vitals homepage.Follow Us on Social MediaTwitter: @Xtalks Instagram: @Xtalks Facebook: https://www.facebook.com/Xtalks.Webinars/ LinkedIn: https://www.linkedin.com/company/xtalks-webconferences YouTube: https://www.youtube.com/c/XtalksWebinars/featured

Moin, wir hatten heute eine Vermisstenmeldung: Jemandem war sein Schwein entlaufen! Sachdienliche Hinweise kamen vom Tierheim Süderstraße, wo das Tier sowohl ärztlich als auch erkennungsdienstlich behandelt wurde: 70 Kilo schwer, schwarz-weiß gemustert, von zutraulichem Charakter. Wie die Geschichte ausging, hört ihr in dieser Folge. Viel Sapß wünscht Maiken Was war heute in Hamburg los? Maiken Nielsen und Ole Wackermann werfen im wöchentlichen Wechsel zum Tagesende einen Blick auf die News und das aktuelle Stadtgeschehen. Das sind die Nachrichten heute Maiken Nielsen am Mittwoch, 31.01.2024 +++GASTRONOMIE IN HAMBURG: GROSSTEIL HAT PREISE ANGEHOBEN+++ Seit einem Monat gilt in der Gastronomie wieder der höhere Mehrwertsteuersatz von 19 Prozent. Viele haben sich ihre Kalkulation noch einmal genauer angeschaut, mit Lieferantinnen und Lieferanten gesprochen und teilweise die Speisen abgeändert. Manche haben jetzt weniger Fleischgerichte auf der Speisekarte, bieten stattdessen mehr vegetarische Variationen an. Welche Erfahrungen die Hamburger Gastronomen im ersten Monat gemacht haben, darüber spricht Maiken Nielsen mit NDR 90,3 Aktuell-Reporterin Karo Weiß.

Vhh

The Minnesota Vikings are 4-5 now after a game with more aggressive play against the Los Angeles Chargers. This week, however, is Packers Week! Aaron Rodgers and his Green Bay Packers come into US Bank Stadium and give the Vikings more opportunity to stay aggressive. More targets to Justin Jefferson, more to Adam Thielen, all against a very good defense. Then there will be the mind games aimed from the vaunted Mike Zimmer offense versus Rodgers and his weapons including Davante Adams, Randall Cobb, and AJ Dillon. There is a special guest, Doogie Wolfson joining the show to talk some scoops from the Minnesota Timberwolves to the Vikings. Als,o Myles Gorham will be stepping in to add to the conversation. Our guest tonight, Doogie rejoins the VHH bar, with Ryan, Myles, and Dave tending, along with you, the fans. Grab your favorite beverage and join the conversation! Discussion topics tonight: Welcome guests (Myles and Doogie) With Doogie Start with Wolves content  - trade rumors - internal turmoil with Towns - where should fans expectations be Move into Vikings content - where's emotions eternally amongst brass - if we move on, who's out? - any interesting storylines internally Once Doogie jumps: Recap last weeks game (quick) - positive takeaways - is this an impressive win? - what's concerning? Jump into this weeks matchup vs Packers - keys to victory - key matchups - can we make a run if we win Lightning round: Over/Under - Rodgers 250 pass yards - AJ Dillon 125 total yards - Vikings Sacks 1.5  (averaging 1.5 over last 4 v Packers) - Kirk ADOT > 7  (7.5 last game, 6.8 all games before last) Buy/Sell - AJ Dillon being a legit starter - Cam Bynum - Mason Cole - Kirks aggressive 4th Q w/ lead Headline Another Diamond In The Rough Here We Go Again… Prediction: Ryan: 6-3 Matt: 4-5 Dave: 4-5 Guest: 5-4 Fan with us!!! Guests Darren “Doogie” Wolfson @DWolfsonKSTP and Myles Gorham @MylesGorham85. The regulars: @Matt Anderson @MattAnderson_8, and Ryan Ortega @sportsguyry, and Producer Dave @Luft_Krigare in the truck. This is a @Climb_ThePocket's & @DailyNorseman's production. _____________________________________________________________________________________ Subscribe to us here! - https://www.youtube.com/c/climbingthepocket Watch it here: https://youtu.be/wRa1Vzg67FM Learn more about your ad choices. Visit podcastchoices.com/adchoices

Koiran ruokaa pitäisi miettiä kokonaisuutena. Piatsi kun se on pakko pilkkoam osasiin. Oikeammin kyse on silloin valikoinnista niistä syistä ja seurauksista miksi jotain ruokaa annetaan koiralle. Silloin ruoka lakkaa olemasta ruokaa koiralle ja käyttötarkoitus onkin olla ravintolisä. Kaksi yleistä esimerkkiä ovat voi ja siirappi, joita kumpaakin kannattaa harkita koiran ongelmissa. Voi: näppärä rasvalisä koiralle Voi on katoavaa kansanperinnettä suomalaisessa keittiössä. Ketoilun ja muiden VHH-ruokavalioiden muodikkuuden myötä voi on hieman palautunut muuhunkin ruokakäyttöön kuin perinteisen leivonnan rasvalisänä, mutta silti kovien rasvojen käyttö vähenee kokonaisosuudesta. Koirille voita ei kannata käyttää perusruokinnassa, koska rasva olisi syytä saada perusruuasta. Mutta voi saattaa olla käyttökelpoinen energialisä, varsinkin suolisto-ongelmaisilla, närästäville tai muutoin rasvarajoitteisilla koirilla. Voi on syystä tai toisesta koirilla kohtuullisen hyvien siedetty. Kukaan ei oikeastaan tiedä miksi, mutta ylipäätään kovat rasvat aiheuttavat vähemmän ongelmia kuin öljyt. Yksi syy siihen saattaa olla niinkin yksinkertainen asia kuin rakenne. Se sulaa ja käyttäytyy kuin muukin ruoka, vaikka taatusti sulaa nopeammin, mutta silti olomuodon muutos juoksevaksi kestää aikansa. Silloin voi (ja muut kovat rasvat) ehtivät eräällä tavalla sekoittua ruokamassaan. Öljyt sen sijaan pysyvät erottuneina pidempään ja juoksevina myös nousevat helpommin eräänlaisena ohivirtauksena vatsalaukun supistuessa. Kun öljy, vatsahappojen takia matalamman pH:n saaneena, pusertuu koiran normaalistikin ihmistä väljemmän ylemmän vatsaportin läpi nieluun, niin se tuntuu närästyksenä. Suurin syy voin sopivuuteen rasvaongelmaisille koirille lienee kuitenkin yksinkertaisempi. Se on maitorasvaa ja ylipäätään koostumukseltaan erilaista kuin lihan tai kasvien rasvat. Lisäksi se mielletään puhtaaksi rasvaksi, mutta ei se ole. Voissa on noin 20 prosenttia vettä joukossa. Maidossa rasva on miselleinä, eräänlaisisan rasvapalloina. Jokaista palloa ympäröi fosfolipideistä ja proteiineista muodostuva kalvo. Kun maito aletaan hapattamaan ja sen jälkeen kirnuamaan, niin nuo kalvot rikotaan. Silloin rasvapallukat alkavat yhdistymään ja aletaan saamaan voita. Nestettä, vettä ja heraa, kaadetaan pois, kirnuamista jatketaan ja loppuvaiheessa voimassaa jopa hieman huudellaan vedellä vaivaamisen yhteydessä, että saadaan viimeisetkin maidon, kerman tai piimän jäämät pois. Voissa rasva on kolmessa eri olomuodossa: vapaana maitorasvana rasvakiteinä alkuperäisinä rasvamiselleinä Tuon kolmikon keskinäiset suhteet vaikuttavat voin rakenteeseen, kuten mitä enemmän rasvaa on kiteinä, niin sitä kovempaa se on. Entisaikoina itsekirnutun voin koostumus riippui siitä miten kukakin maitoa oli käsitellyt, ja kuinka paljon voihin jätettiin vettä. Teollinen voin valmistus tuottaa melkoisen tasalaatuista jälkeä, ja jos eroja on, niin se löytyy enemmältkin meijereiden väliltä, ei meijerien omien erien väliltä. Tuo kaikki yhdessä - rasvan rakenne, rasvahappokoostumus sekä vesimäärä - selittävät hyvin pitkälle miksi voi on usein vatsaystävällisempää kuin läski tai öljy. Voi eroaa rasvakoostumukseltaan jonkun verrankin niin öljyistä kuin lihankin rasvoista. Jotta asia olisi hieman hankalampaa, niin rasvakoostumus eroaa myös naudoittain ja varsinkin millaista rehua ne ovat saaneet. Mutta koska nautojen pitäminen ja ruokkiminen on hyvin samanlaista kaikilla navetoilla, niin ravinnolla ei ole suurtakaan käytännön merkitystä voin rasvakoostumukseen. Paitsi jos joku meijeri päättää haluta tuottaa hieman toisenlaista maitoa rasvan suhteen. Siirappi: nopeaa energiaa koiralle Siirappi on nopeita hiilihydraatteja. Se on niiden voima koiran ruokinnassa, sekä suurin syy omistajille olla käyttämättä sokereita. Ihmisten ylipainon ja ahneuden takia hiilihydraatit on demonisoitu ja niiden potentiaali jätetään hyödyntämättä silloin kun edut koiralle olisivat selvät. Siirappi säilyy, on kohtuullisen edullista ja helposti annosteltavaa. Sen koostumuksesta iso osa on glukoosia, joka imeytyy suoraan. Loput ovat muuten vain yksinkertaisia ja helposti hyödynnettäviä sokereita. Siirapilla haetaan niitä kahta ainoaa asiaa, jonka takia hiilihydraatteja ylipäätään syödään: energiaa ja insuliinin nostamista. Toki kumpikin onnistuu ilmankin sokereita, mutta nopeus ei ole sama. Lisäksi yksinkertaisilta sokereilta puuttuvat usein ne haitat, jonka takia koira ei kykene syömään riittävästi rasvaa - närästys ja suolisto-oireilu. Koiran ruokaa katsotaan aina laajemmin+ Voi ja siirappi ovat hyelppoja esimerkkjä siitä miten ruokaa on mietittävä laajemmin kuin uskomusten tai jonkun yhden nimetyn ominaisuuden kautta. Toki se vaatii erilaisten asioiden syvempää osaamista ja perusteita parempaa osaamista, mutta melkoinen osuus tuostakin nippelistä löytyy jo pelkän perustiedon kautta. Niitä ei vain osata tai tajuta kytkeä koiran ruokintaan. Podcastissä mainitut Kaffepaussissa viitattiin pariin jo julkaistuun juttuun: Rasva, kalaöljy vai omega-3 koiralle tapaus Roima ja Faunatar Puhelinajan varaaminen

Join us for the Climbing The Pocket Network's and Daily Norseman's “Vikings Happy Hour.” The Minnesota Vikings will be hosting the Denver Broncos for preseason week 1. VHH is giving away some tickets for the game. Topics of the day from joint practice at training camp, the unofficial depth chart, kicker concerns, and more are to be topics at the bar. Matt, Ryan, and Myles be having fun so join the conversation and have fun too. Grab yourself something to sip

FMD serological blocking ELISA based on VHH for post-vaccination monitoring

C. Alvarez - FMD serological blocking Elisa based on VHH for post-vaccination monitoring. by European Commission for the Control of FMD

En #LaMañanaInformativa por #UNCB Radio, hablamos con Juan Pablo Malito, biotécnico del INTA, sobre la investigación que el INTA, el Ministerio de Agricultura, Ganadería y Pesca y del CONICET, Ministerio de Ciencia, Tecnología e Innovación, llevaron a cabo hasta la lograr la obtención de los nanoanticuerpos VHH provenientes de las llamas y los anticuerpos IgY, derivados de la yema de los huevos de gallina, con capacidad de neutralizar la infección por coronavirus. #LaVozDeLosClubesDeBarrio #UNCB #UNCBRADIO

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2020.09.01.278853v1?rss=1 Authors: Lupardus, P. J., Rokkam, D. Abstract: CD45 is an abundant and highly active cell-surface protein tyrosine phosphatase (PTP) found on cells of hematopoietic origin. CD45 is of particular importance for T-cell function, playing a key role in the activation/inactivation cycle of the T-cell receptor signaling complex. The extracellular domain of CD45 is comprised of an N-terminal mucin-like domain which can be alternatively spliced to a core domain (RO) consisting of four domains with fibronectin 3 domain (FN3)-like topology. The study of CD45 has been hampered by a small set of publicly available antibodies, which we characterized as specific to the N-terminal FN3 domains of CD45 RO. To broaden the human CD45 reagent set, we identified anti-CD45 single domain VHH antibodies from a post-immune llama phage display library. Using a yeast display domain mapping system and affinity measurement we characterized seven unique clonotypes specific for CD45 RO, including binders that target each of the four FN3-like domains. These VHH molecules are important new tools for studying the role of CD45 in T-cell function in vitro and in vivo. Copy rights belong to original authors. Visit the link for more info

Corona-Massentests, antivirale Nasensprays, ein möglicher Impfstoff. Startups aus Österreich kämpfen gegen Covid19. Wir stellen euch die spannendsten vor. Alexander Seitz könnte ganz Österreich an einem Tag auf Covid-19 testen. Sein Startup "Lexogen" nutzt eine neue Methode, um das Coronavirus im Menschen nachzuweisen. "Bei uns bekommt jede Probe eine Art Barcode", so Seitz. Die WissenschafterInnen müssten dann nicht mehr jeden Test einzeln auswerten - was lange dauert - sondern könnten alle Proben gleichzeitig analysieren. Theoretisch Millionen auf einmal. Lexogen ist eines von mehreren vielversprechenden Startups, die im "Vienna Health Hub" (VHH) im 3. Wiener Bezirk angesiedelt sind. Der Health Hub ist ein "Accelerator". Er unterstützt besonders innovative Unternehmen aus dem medizinischen Bereich, ein Produkt zu entwickeln. VHH-CEO Irene Fialka kann eine ganze Menge von ihnen aufzählen, die sich jetzt dem Kampf gegen das Coronavirus bzw. Covid-19 verschrieben haben. Sieben von ihnen werden im Rahmen des "Corona Emergency Calls" der Bundesregierung finanziell unterstützt. Welche österreichischen Ideen gegen Corona könnten die Welt verändern? Ihr hört es in "Start Me Up".

Moikka. Vastaan 10 kysymykseen ketogeenisestä ruokavaliosta. Kysymysten aiheet: 1. Keto ja lisämunuaisten uupuminen, sopiiko yhteen? 2. Kannattako ketoilla jos kuormittava elämäntilanne? 3. Onko Skyr maitorahka ok ketolla? 4. Eikö kalorit jää vajaaksi pahasti jos paastoaa ja on ketoosissa? 5. Voiko keton toteuttaa ilman maitotuotteita? 6. Kauanko ketoosiin pääseminen kestää ja koska se ketoflunssa tulee? 7. Keto ja kilpirauhsen vajaatoiminta? 8. Voiko ketolla kasvattaa lihasta? Entäs kovaa treenaaminen? 9. Mitä kaikkea 30 päivän ketohaasteeseen kuuluu ilman lisäosia? 10. Vhh ja keto erot? Olen saanut yli 100 kysymystä viikon aikana pelkästään instagramiin liittyen ketoon ja ensi viikolla alkavaan valmennukseen. Kiitos näistä ja sori etten pysty ihan kaikkiin henk.kohtaisesti vastaamaan!! Mutta näiden videoiden kautta pystyn kuitenkin auttamaan monia, koska samat kysymykset toistuvat usein. Tänään oli sopiva hetki laittaa kamera pyörimään ja jutella ihan vapaalla flowlla aiheesta. Oli ilo vastata teidän kysymyksiin ja toivottavasti saat tästä videosta paljon irti ja opit lisää. Jos tämän kaltaisista videoista on apua ja saat niistä lisäarvoa niin klikkaa peukkua niin tiedän tehdä enemmän. Mukavaa päivää. Tomi Kokko Ps. Uudistettu 30 päivän ketohaaste alkaa ma 11.5. ja ennätysmäärä porukkaa jo ilmoittautunut. Hinta alkaen 49€! Mukaan pääset täältä: https://tomikokko.com/ketovalmennus/

Talkin’ Hock­ey sea­son 2 episode 21 — we are back in the vir­tu­al bunker talk­ing some real hock­ey here on Gordie ​“Mr. Hock­ey” Howe’s birth­day — born in Flo­ral, SK.The boyce catch up and talk goalie fights (Youtube Potvin vs Hex­tall, John­son vs DiP­i­etro, and Emery vs Biron), they talk about the NHLPA play­er’s poll results and the VHH and Local Leg­end is a two-fer again with Bran­don-born Bill Ranford!The tunes this week come from Kath­leen Edwards and our beer league bud­dy Pip Skid (Germ Warfare)The boyce will be back next week reveal­ing their ulti­mate line-ups of Jets 1.0 and 2.0 players.Keep your stick on the ice and watch out for the goalie’s blocker!

All right, here we go now! Talkin’ Hock­ey sea­son 2 episode 18 — Let’s Go!!! On this weeks episode, co-hosts Tom and Randy talk about how there’s no time like the present for a Jets Heater … their play­off hopes are dwin­dling. The boys also got the spread­sheets going and do an in depth com­par­i­son of Neil Pio­nk vs Jacob Trou­ba, a cou­ple guys who trad­ed places this year. The Local Leg­end and Vin­tage Hock­ey Hair Play­er of the week is New York Islanders leg­end, and St. Boni­faces’ own, Butch Gor­ing! Sec­ond show in a row that the VHH & LL are the same play­er.… coin­ci­dence? I think not. We’ve got some Barn­ham­mer beer and a killer tune by The Hex­talls on this weeks show too, so tune in. You won’t regret it! #pucks­deep #stick­on­the­ice

Show #675   Good morning, good afternoon and good evening wherever you are in the world, welcome to EV News Daily for Thursday 23rd January 2020. It’s Martyn Lee here and I go through every EV story to save you time.   Thank you to MYEV.com for helping make this show, they’ve built the first marketplace specifically for Electric Vehicles. It’s a totally free marketplace that simplifies the buying and selling process, and help you learn about EVs along the way too.   FORMER FORD CEO: EVS ARE THE FUTURE The former Ford CEO Mark Fields was on CNVC this week and says that EVs are the future. Given that isn't up for debate, the big questions which remains are when automakers get payback and how fast will EV adoption happen?   I've got three clips to play you and this is fascinating, someone who lived and breathed the issues which we talk about, but he has more first hand experience than most others.   On Elon Musk creating demand and how Tesla can be profitable ...   On automakers trying to do what Tesla has done because of their legacy costs...   On EV's being a computer on wheels plus autonomy...   So what didn't he talk about? Well he could have named the issues which used to face Ford and what it faces now. He didn't name other automakers. He didn't specially say Tesla's were better although he raved about their software.   https://www.youtube.com/watch?time_continue=1&v=0pD3RCcdQ2c&feature=emb_title   TESLA MODEL Y DELIVERY RUMORS "There’s been some chatter on the interwebs that some Model Y reservation owners have received calls confirming their Model Y orders and telling them where their deliveries will occur." reports Zach at CleanTechnica: "One person claims that the Tesla staff member who talked to him or her indicated that a Model Y Performance could be delivered next month, in February, whereas a Model Y Long Range AWD could be delivered in March or April. This reservation holder reportedly resides in Ontario."   But...use REddit as a source at your peril. This user has been active since 2016.   Also, that would make it a Founders Edition. Also, first deliveried in Canada? Really?   https://cleantechnica.com/2020/01/23/tesla-model-y-delivery-rumors/   TESLA TOPS $100 BILLION "Tesla shares surged to a fresh all-time high Wednesday, lifting Elon Musk's clean-energy carmarker past the $100 billion mark and extending a run that has taken his company past the likes of Ford, General Motors and Volkswagen in less than a decade." says TheStreet.com: "Tesla shares were marked 8.12% higher Wednesday to change hands at $591.78 each, an all-time high that would lift the stock to a market value of just over $106.6 billion and extend its rise since the June 3 nadir to around 230%. Tesla's market value, in fact, overtakes that of Volkswagen AG at the start of trading later today, with Europe's largest carmarker languishing around the $100.7 billion mark. Japan's Toyota Motor Co., (TM) - Get Report however, remains the world leader with a market value of around $198 billion."   https://www.thestreet.com/investing/tesla-tops-100-billion-lifts-market-value-past-volkswagen   700 JAGUAR I-PACE EVS TO JOIN NHS FLEET "Jaguar Land Rover and the National Health Service have teamed up with an unprecedented deal that will see a fleet of 700 Jaguar I-Pace electric cars be made available for public sector staff all over Britain The agreement, which came via Northumbria Healthcare’s NHS Fleet Solutions, will allow staff from over 200 organisations to lease the I-Paces over a three-year period. The move helps the NHS keep to its plan of reducing its carbon footprint." says Motor1.com: "The fleet of I-Paces will be available to any public sector staff member who works for organisations that are signed up to NHS Fleet Solutions from April 2020. Payment for the cars will come directly out of employee salaries."   https://uk.motor1.com/news/394109/nhs-i-pace-leases/   BMW I VENTURES INVESTS IN SOFTWARE MOTOR COMPANY "BMW i Ventures has invested in Software Motor Company (SMC), a Silicon Valley-based company developing reliable, efficient and intelligent motor systems." reports Green Car Congress: "Electric vehicles will require lower costs and longer ranges to reach ubiquity. SMC’s technology enables lower cost motors, and lowers the cost of the battery for comparable range, thanks to higher efficiency over a wide operating range."   Greg Smithies of BMW i Ventures: "Electric motors are a massive, $100B market, and they move our modern world, while consuming over 45% of the world's electricity. So investing in a company that can deliver huge electrical cost savings, while selling motor systems that are lower cost, more reliable, and made from more sustainable materials seems like a no-brainer."   https://www.greencarcongress.com/2020/01/20200123-smc.html   OPEL GRANDLAND X HYBRID4 "Autogefühl recently had an opportunity to test drive the crossover SUV Opel Grandland X Hybrid4 (Vauxhall Grandland X Hybrid4 in the UK), which will have also two siblings: Peugeot 3008 GT Hybrid4 and Citroën C5 Aircross SUV Hybrid." says InsideEVs: "This all-wheel-drive plug-in hybrid (the front-wheel driving version coming too) is one of the first attempts from Opel at plug-ins under the PSA Group. 50 km (31 miles) of WLTP all-electric range (60 km NEDC) 13.2 kWh battery all-wheel drive 220 kW/300 hp of system output: 147 kW/200 hp 1.6L PureTech combined with the new e-EAT8 8-speed automatic transmission (Electric Efficient Automatic Transmission) and 80 kW/110 hp electric motor in the front plus another 80 kW/110 hp electric motor for rear axle charging using 3.3 kW on-board charger in around 4h or around 2h using 6.6 kW optional charger"   https://insideevs.com/reviews/394351/opel-grandland-x-hybrid4-featured-autogefuhl/   ELECTRIFY AMERICA INVESTS $1.3 MILLION IN ENERGY STORAGE PROGRAM "Electrify America will invest $1.3 million in the Sacramento Municipal Utility District’s (SMUD) Energy StorageShares program, which incentivizes energy storage in grid-stressed locations throughout the city." reports ChargedEVs.com: "Through the program, Electrify America will receive recurring credits for the demand reduction needs at its 12 charging stations powered by SMUD in the Sacramento area."   SMUD CEO Arlen Orchard: "This program will help address peak energy demands, minimize impacts to the grid, and support the expansion of charging in our community."   https://chargedevs.com/newswire/electrify-america-invests-1-3-million-in-energy-storage-program/   OLD PHEV BATTERIES GET SECOND LIFE AT EV BUS CHARGING STATION In the Bergedorf quarter of Hamburg, a container with a total capacity of 500 kWh has been connected to the depot charging network at the Verkehrsbetriebe Hamburg-Holstein (VHH) depot. There, MAN Truck & Bus and VHH want to work together to test how used batteries behave after initial use in vehicles and as a stationary energy storage system, under real-world operating conditions. A white container containing 50 batteries – which were previously installed in VW Passat GTE vehicles – is located here. Each battery has a nominal capacity of 9.9 kWh, meaning that the container has a total capacity of exactly 495 kWh – about half a megawatt. The batteries are mounted on racks and then interconnected via battery management to form a large battery. One objective of the project is to develop a flexible battery storage concept which allows for the replacement of batteries.     ELECTRIC CAR OWNERSHIP IS THE CURE FOR RANGE ANXIETY "Owning an electric car actually eases those anxieties. AAA released its latest EV study on Wednesday and it found car buyers shouldn't dismiss battery-powered cars until they actually live with one." reports CNET: "After surveying 40,000 electric vehicle owners, plus another 1,000-plus plug-in hybrid owners, AAA concluded that owning an EV is the best prescription for the unknown factors surrounding them. In total, 71% of those surveyed were first-time EV buyers, and 96% of them said they would shop for another EV. 43% of them said they drive more now that they own an EV. On average, 75% of owners just charge their cars at home. The average EV driver goes 39 miles per day. AAA asked if after owning an EV they felt better about these fears. The majority, 77%, said yes and were less or no longer concerned about range."   https://www.cnet.com/roadshow/news/electric-car-ownership-range-charging-study/   87 POUNDS OF AMMUNITION, BOMBS FOUND AT TESLA'S 'GIGAFACTORY 4' SITE NEAR BERLIN "fficials in the German state of Brandenburg have discovered 187 pounds of World War II-era ammunition at the site of Tesla’s planned factory near Berlin. The Maerkische Oderzeitung German newspaper said as many as 25 unexploded bombs could be located within the area." according to IBTimes: "On Tuesday, thousands of German workers were forced to evacuate after a bomb weighing over 1,100 pounds was found in the western German city of Cologne. On the same day, authorities defused another bomb in central Berlin."   https://www.ibtimes.com/world-war-ii-aftermath-187-pounds-ammunition-bombs-found-teslas-gigafactory-4-site-2907631     NEW QUESTION OF THE WEEK The MYEV.com Question Of The Week…   With increasing attacks on EVs and vandalism caught on camera, why do some people have a problem with EVs?     I want to say a heartfelt thank you to the 230 patrons of this podcast whose generosity means I get to keep making this show, which aims to entertain and inform thousands of listeners every day about a brighter future. By no means do you have to check out Patreon but if it’s something you’ve been thinking about, by all means look at patreon.com/evnewsdaily   You can listen to all 674s previous episodes of this this for free, where you get your podcasts from, plus the blog https://www.evnewsdaily.com/ – remember to subscribe, which means you don’t have to think about downloading the show each day, plus you get it first and free and automatically.   It would mean a lot if you could take 2mins to leave a quick review on whichever platform you download the podcast.   And if you have an Amazon Echo, download our Alexa Skill, search for EV News Daily and add it as a flash briefing.   Come and say hi on Facebook, LinkedIn or Twitter just search EV News Daily, have a wonderful day, I’ll catch you tomorrow and remember…there’s no such thing as a self-charging hybrid.     PHIL ROBERTS / ELECTRIC FUTURE (PREMIUM PARTNER) BRAD CROSBY (PREMIUM PARTNER) AVID TECHNOLOGY (PREMIUM PARTNER)   DAVID ALLEN (PARTNER) OEM AUDIO OF NEW ZEALAND AND EVPOWER.CO.NZ (PARTNER) PAUL O’CONNOR (PARTNER) TRYEV.COM (PARTNER) GARETH HAMER eMOBILITY NORWAY HTTPS://WWW.EMOBILITYNORWAY.COM/  (PARTNER) ALAN ROBSON (EXECUTIVE PRODUCER) ALAN SHEDD (EXECUTIVE PRODUCER) ALEX BANAHENE (EXECUTIVE PRODUCER) ALEXANDER FRANK @ https://www.youtube.com/c/alexsuniverse42 ANDERS HOVE (EXECUTIVE PRODUCER) ANDREA JEFFERSON (EXECUTIVE PRODUCER) ASEER KHALID (EXECUTIVE PRODUCER) ASHLEY HILL (EXECUTIVE PRODUCER) BÅRD FJUKSTAD (EXECUTIVE PRODUCER) BARRY PENISTON (EXECUTIVE PRODUCER) BRENT KINGSFORD (EXECUTIVE PRODUCER) BRIAN THOMPSON (EXECUTIVE PRODUCER) BRIAN WEATHERALL (EXECUTIVE PRODUCER) BRUCE BOHANNAN (EXECUTIVE PRODUCER) CHARLES HALL (EXECUTIVE PRODUCER) CHRIS HOPKINS (EXECUTIVE PRODUCER) COLIN HENNESSY (EXECUTIVE PRODUCER) CRAIG COLES (EXECUTIVE PRODUCER) CRAIG COOPER (EXECUTIVE PRODUCER) CRAIG ROGERS (EXECUTIVE PRODUCER) DAMIEN DAVIS (EXECUTIVE PRODUCER) DAN FAIRS (EXECUTIVE PRODUCER) DARREN BYRD (EXECUTIVE PRODUCER) DARREN FEATCH (EXECUTIVE PRODUCER) DARREN SANT FROM YORKSHIRE EV CLUB (EXECUTIVE PRODUCER) DAVE DEWSON (EXECUTIVE PRODUCER) DAVID BARKMAN (EXECUTIVE PRODUCER) DAVID FINCH (EXECUTIVE PRODUCER) DAVID PARTINGTON (EXECUTIVE PRODUCER) DAVID PRESCOTT (EXECUTIVE PRODUCER) DEREK REILLY FROM THEEFFECT.NET (EXECUTIVE PRODUCER) DON MCALLISTER / SCREENCASTSONLINE.COM (EXECUTIVE PRODUCER) ENRICO STEPHAN-SCHILOW (EXECUTIVE PRODUCER) FREDRIK ROVIK (EXECUTIVE PRODUCER) FREEJOULE AKA JAMES (EXECUTIVE PRODUCER) GENE RUBIN (EXECUTIVE PRODUCER) GEOFF LOWE (EXECUTIVE PRODUCER) IAN GRIFFITHS (EXECUTIVE PRODUCER) IAN SEAR (EXECUTIVE PRODUCER) JACK OAKLEY (EXECUTIVE PRODUCER) JAMES STORR (EXECUTIVE PRODUCER) JERRY ALLISON (EXECUTIVE PRODUCER) JIM MORRIS (EXECUTIVE PRODICERS) JOHN BAILEY (EXECUTIVE PRODUCER) JOHN LACEY FROM CLICK CLACK VIDEO NZ (EXECUTIVE PRODUCER) JON AKA BEARDY MCBEARDFACE FROM KENT EVS (EXECUTIVE PRODUCER) JON KNODEL (EXECUTIVE PRODUCER) JUAN GONZALEZ (EXECUTIVE PRODUCER) KEN MORRIS (EXECUTIVE PRODUCER) KEVIN MEYERSON (EXECUTIVE PRODUCER) KYLE MAHAN (EXECUTIVE PRODUCER) LARS DAHLAGER (EXECUTIVE PRODUCER) LAURENCE D ALLEN (EXECUTIVE PRODUCER) LEE BROWN (EXECUTIVE PRODUCER) LUKE CULLEY (EXECUTIVE PRODUCER) MARCEL LOHMANN (EXECUTIVE PRODUCER) MARCEL WARD (EXECUTIVE PRODUCER) MARK BOSSERT (EXECUTIVE PRODUCER) MARLIN SCHELL (EXECUTIVE PRODUCER) MATT PISCIONE (EXECUTIVE PRODUCER) MATTHEW ELLIS (EXECUTIVE PRODUCER) MAZ SHAR (EXECUTIVE PRODUCER) MIA OPPELSTRUP (EXECUTIVE PRODUCER) MICHAEL PASTRONE (EXECUTIVE PRODUCER) MICHEAEL KYFFIN (EXECUTIVE PRODUCER) MIKE ROGERS (EXECUTIVE PRODUCER) MIKE WINTER (EXECUTIVE PRODUCER) NATHAN GORE-BROWN (EXECUTIVE PRODUCER) NEIL E ROBERTS FROM SUSSEX EVS (EXECUTIVE PRODUCER) NORTHERN EXPLORERS (EXECUTIVE PRODUCER) OHAD ASTON (EXECUTIVE PRODUCER) PAUL RIDINGS (EXECUTIVE PRODUCER) PAUL SHELLEY ((EXECUTIVE PRODUCER) PAUL STEPHENSON (EXECUTIVE PRODUCER) PERRY SIMPKINS (EXECUTIVE PRODUCER) PETE GLASS (EXECUTIVE PRODUCER) PETE GORTON (EXECUTIVE PRODUCER) PETER & DEE ROBERTS FROM OXON EVS (EXECUTIVE PRODUCER)  PHIL MOUCHET (EXECUTIVE PRODUCER) PONTUS KINDBLAD (EXECUTIVE PRODUCER) RAJEEV NARAYAN (EXECUTIVE PRODUCER) RALPH JENSON (EXECUTIVE PRODUCER) RENÉ SCHNEIDER (EXECUTIVE PRODUCER) ROB COOLING / HTTP://WWW.APPLEDRIVING.CO.UK/ (EXECUTIVE PRODUCER) ROB HERMANS (EXECUTIVE PRODUCER) ROBERT GRACE (EXECUTIVE PRODUCER) ROBIN TANNER (EXECUTIVE PRODUCER) RUPERT MITCHELL (EXECUTIVE PRODUCER) SABBY THE CAT (EXECUTIVE PRODUCER) SARI KANGASOJA (EXECUTIVE PRODUCER) SEIKI PAYNE (EXECUTIVE PRODUCER) STEVE JOHN (EXECUTIVE PRODUCER) STUART HANNAH (EXECUTIVE PRODUCER) THOMAS J. THIAS  (EXECUTIVE PRODUCER) THE PLUGSEEKER – EV YOUTUBE CHANNEL (EXECUTIVE PRODUCER) TIM GUTTERIDGE (EXECUTIVE PRODUCER) WILLIAM LANGHORNE (EXECUTIVE PRODUCER) ZACK HURST (EXECUTIVE PRODUCER)     CONNECT WITH ME! EVne.ws/itunes EVne.ws/tunein EVne.ws/googleplay EVne.ws/stitcher EVne.ws/youtube EVne.ws/iheart EVne.ws/blog EVne.ws/patreon   Check out MYEV.com for more details: https://www.myev.com

Tämän podcastin aiheena on ketogeeninen ruokavalio “työkaluna” elämässä! Vieraana lääkäri Olli Sovijärvi, jonka kanssa keskustelu työkaluista lähti hieman laukalle kuten podilla saattaa aistia. Sen vuoksi pidetään “työkalut” tässä vaiheessa lainausmerkeissä!! Hauskaa oli ja kyllä me päästiin itse aiheisiinkin käsiksi. Puhuttiin mm. ❥ Miten ketogeeninen ruokavalion vaikuttaa veriarvoihin? ❥ Entäs ketogeeninen ruokavalio ja kolesteroliarvot? ❥ Mitkä ovat VHH ruokavalion ja keton erot? ❥ Miten keto vaikuttaa ruokahaluihin ja makeanhimoihin? ❥ Mitä tarkoittaa metabolinen joustavuus ja miksi se on todella tärkeää? ❥ Miksi monet ovat ruoan “orjia”? ❥ Kuinka keto voi auttaa monia pääsemään lähemmäksi sisäisiä pelkoja/haasteita? Koko jakso löytyy myös videona YouTube kanavaltani. Laitahan kuunteluun tai katseluun, opi ja inspiroidu. Haluatko kokeilla ketogeenistä ruokavaliota ja oppia aiheesta lisää? Tervetuloa mukaan uuteen “30 päivän ketohaaste” valmennukseeni, jossa lääkäri Olli Sovijärvi on myös mukana. Olemme kuvanneet kurssille paljon hyvää materiaalia, pidämme kädestäsi kiinni jotta voit kokeilla ketoosia tehokkaasti ja turvallisesti. Materiaalit ovat pysyvästi käytössä, joten mukaan vaan vaikka et pääsisikään heti aloittamaan. Tutustu tarkemmin https://tomikokko.com/ketovalmennus/ Erikoiskiitos vielä Ollille vierailusta uusilla mestoillani. Always a pleasure with this guy!!!

A clinic participant asked a great question: “What do you do about a horse that ‘sets back’ when tied?” In this episode we talk about this from the perspective of safety and understanding the horse’s instincts, and offer specific suggestions about how to avoid the problem, and how to address it in a horse that’s already developed the habit. Preventing–or Curing–the Horse that “Sets Back” When Tied Some horses react badly to being tied up, using all their strength to pull against the restraint. Can this be prevented? Can it be “cured”? Why does a horse pull back when tied? It’s because the horse doesn’t respect the halter, so it all starts with proper halter training. Flight or fight response The key to understanding this problem is understanding the horse’s instincts. By evolutionary development, a horse is a prey animal whose first, preferred response to any threat is to flee–get away first, check it out later. When we tie up our horses, we take that defensive option away, leaving them only with their second choice: to fight. The objective of early training is to teach the horse to overcome that instinct to flee in response to the pressure they feel when tied. Prevention is best The keys to preventing this habitual response are: 1. Due diligence (understanding the horse’s natural, instinctive responses) and careful training. In this episode we outline the process of training the horse to respect the halter. 2. Don’t provoke the flight or fight instinct by tying too hard and fast (use a knot with some give). It’s a safety issue This response of setting back, or pulling back, is dangerous for us, for our equipment, and for our horses. We discuss some safety tips: 1.  Use a knot that can easily be untied. Check back here for a short instructional video that demonstrates how to tie the knot I recommend. 2.  Use a halter and lead rope with no metal fittings that can break. 3.  Always carry a safe type of knife that can quickly be used to cut the rope if necessary. Check back here for a photo of what I carry for this purpose.   What do you think? We want to hear your questions, comments, and suggestions. Share them in the comments section below, reach out to us on the Van Hargis Horsemanship Facebook page, or email me! We want your suggestions for topics we can address in future episodes, so if there’s a particular area where you’re struggling, or just curious, let us know! We’d Love to Have Your Help Spreading the Word If you enjoy this podcast and think others would like it too, please consider rating and reviewing Ride Every Stride in iTunes. It helps make the show more visible, so more people can find it, and the feedback lets us know if we’re on the right track. Thank you–your support is so appreciated. Next Time on Ride Every Stride We’ll begin talking about the most important element of training: ground work. If you don’t want to miss it, be sure to subscribe in iTunes or on Stitcher. Thank you for listening. Until next time . . . remember to Ride Every Stride! Van Van Hargis Horsemanship

In the early episodes of Ride Every Stride we’re working on laying a foundation for what comes later. As I’ve said many times over the years, when it comes to horsemanship (and maybe life?), there’s nothing beyond the basics. For horsemanship, those basics are what I call the Absolute Basic Criteria. Picking up where we left off last time, in this episode of Ride Every Stride we’re talking about the second of those ABCs. The Absolute Basic Criteria of Horsemanship, part 2 — Control of Forward Movement As we said last week, even the most complex, sophisticated maneuvers on horseback are refinements of the basics. Understanding those basics–the ABCs of horsemanship–will equip us to work with our horses to accomplish whatever our horsemanship goals might be. What Are the ABCs of Horsemanship? forward movement (we discussed this in episode 2) control of forward movement stopping of forward movement (we’ll discuss this next time)   Once we’ve achieved the first of the ABCs–forward movement–we want to be able to control that forward movement, both its direction and its speed. In this episode we talk about what cues we use, when, and why. Directional Control One component of controlling forward movement is controlling its direction. We want to be able to turn our horses and get them to go where we want them to go. In learning the techniques for doing this, remember: our hands control what happens from our horse’s shoulders (withers) forward, and our legs control what happens from the withers back.   There’s no magic spot where your hands should be–you need to pay attention to your horse and make small adjustments as needed to get the response you want. Both by instinct and, hopefully, by good training, the horse will be inclined to move away from pressure, so think: what do I need to do to create an opening for my horse to go where I want him to go? We use the image of a river flowing from the horse’s hindquarters (where all the power comes from), through your legs (“big rocks on the banks of the river”) forward to the bit. You can control the horse’s movement–the flow of the river–by moving the rock out of the way on the side you want the horse to go. In other words, if you want the horse to go right, it’s not that you push from the left, but that you take the pressure away from the right, opening a pathway for the horse to go where you want it to go. Speed Control Although beginning riders are concerned about a horse running off with them, I’m more concerned about a horse that won’t move, in part because of safety issues (like the story I tell about a confrontation between a well behaved, but a bit lazy, horse I was riding and a cow). I want my horses to be calm and well behaved, but when I signal that it’s time to go, they need to respond immediately. To train that habit, I use the approach I call “squeeze, bump, kick, and kill.” For your safety’s sake, you need to understand the concepts of speed (and directional) control we talk about in this episode. Use the horse’s instinctive desire to conserve energy to help her learn to follow your cues (make it harder for her to go at the pace she wants to go if it’s faster than you want her to go) Learn how to make sure you are the one establishing the rhythm, which helps set the pace   What do you think? We want to hear your questions, comments, and suggestions. Share them in the comments section below, reach out to us on the Van Hargis Horsemanship Facebook page, or email me! We want your suggestions for topics we can address in future episodes, so if there’s a particular area where you’re struggling, let us know! We’d Love to Have Your Help Spreading the Word If you enjoy this podcast and think others would like it too, please consider rating and reviewing Ride Every Stride in iTunes. It helps make the show more visible, so more people can find it, and the feedback lets us know if we’re on the right track. Thank you–your support is so appreciated. Next Time on Ride Every Stride We’ll talk about the next component of the Absolute Basic Criteria of Horsemanship: stopping of forward movement. If you don’t want to miss it, be sure to subscribe in iTunes or on Stitcher. Thank you for listening. Until next time . . . remember to Ride Every Stride! Van Van Hargis Horsemanship      

As I’ve said many times over the years, when it comes to horsemanship (and maybe life?), there’s nothing beyond the basics. For horsemanship, those basics are what I call the Absolute Basic Criteria. In this episode of Ride Every Stride we’re talking about the first of those ABCs. The Absolute Basic Criteria of Horsemanship Even the most complex, sophisticated maneuvers on horseback are refinements of the basics. Understanding those basics–the ABCs of horsemanship–will equip us to work with our horses to accomplish whatever our horsemanship goals might be. What are the ABCs of Horsemanship? forward movement control of forward movement stopping of forward movement   Although most beginning riders are especially concerned about the last of those ABCs–stopping–we actually want our horses to move. Forward movement generally constitutes one of the three basic gaits: the walk, the trot, or the lope (or canter). If we understand the mechanics of each gait, we can cooperate with our horse to make it easier for her to move forward easily at the gait we want. In this episode Van shares a simple tip for knowing where your horse’s feet are without leaning over to look. Using this method keeps you centered on your horse’s back, which is safer for you and more comfortable for your horse, and helps you use a simple technique to encourage that forward movement that’s the starting point to accomplishing our horsemanship goals. Ride Every Stride is about empowering you with knowledge. Understanding the mechanics of how your horse moves is key to letting you work with your horse instead of against him.  Knowledge of how your horse works (and thinks) leads to greater confidence, which leads to greater enjoyment. What do you think? We want to hear your questions, comments, and suggestions. Share them in the comments section below, reach out to us on the Van Hargis Horsemanship Facebook page, or email me! We’d Love to Have Your Help If you enjoy this podcast and think others would like it too, please consider rating and reviewing Ride Every Stride in iTunes. It helps make the show more visible, so more people can find it. Thank you–your support is so appreciated. Next Time on Ride Every Stride We’ll talk about the next component of the Absolute Basic Criteria of Horsemanship: control of forward movement. If you don’t want to miss it, be sure to subscribe in iTunes.  Thank you for listening. Until next time . . . remember to Ride Every Stride! Van Van Hargis Horsemanship

It’s been months in the planning, but we are excited to finally launch Ride Every Stride, a podcast about horsemanship . . . and more. In episode 1 we share a little bit about who I am, what my horsemanship background is, and what you can expect from me and this podcast. In this and future episodes, you’ll get lots of practical horsemanship tips–tools you can take with you to the barn and put to use to improve your working relationship with your horse. But I hope you’ll also be inspired as we talk about the many ways the concepts and principles we use to improve our horsemanship also apply to our journey as humans. As I’ve said many times, horsemanship is an opportunity to practice humanship every day. My purpose in creating this podcast is simple: to serve you. I can do that best if you let me know how. What questions do you have? What challenges are you facing in your growth as a horseman or horsewoman? What topics would you like to hear me cover in future episodes? I want to hear from you, so please share your questions, comments, and suggestions either in the comments below, on the Van Hargis Horsemanship Facebook page, or by emailing me. And to make sure you don’t miss a single episode of Ride Every Stride–subscribe in iTunes. I’m excited to start this new phase of my journey, and I hope you’ll come along for the ride. Until next time, remember: it’s your life, it’s your journey, it’s your trail . . . so Ride Every Stride. Van

CompactFitin bodyweight coach -leirin puimista ja sitten kysymykset: “Moi! Ja kiitos mielenkiintoisesta podcastista! Kysymykseni koskee tankkaamista pitkäkestoiseen suoritukseen ja sen aikana. Mitä suosittelette tankkaukseen, jos haluaa noudattaa paleo-tyyppistä ja nk. hiilihydraattitietoista ruokavaliota ja välttää turhia sokereita myös ennen suorituksia ja niiden aikana. Ketoosiin ei ole tarkoitus pyrkiä eikä noudattaa VHH dieettiä vaan välttää keinotekoisia hiilihydraattilähteitä myös urheillessa. Lihakset ja maksahan ottavat vain rajoitetun määrän hiilareita vastaan, joten energiaa pitäisi saada irti rasvoista. Kuulisin mielellänne ajatuksianne tankkaamisesta ennen urheilusuoritusta ja mitä tehdä, jos tuntuu, että puhti loppuu suorituksen aikana. Laji on siis suunnistus ja pitkä suoritus on yli kahden lenkki tunnin rata á 15 km. Lyhyemmät pyrähdykset 5-7 km menevät ilman suurempia tankkaamisia. Suoritukset eivät ole tasaista jolkottelua vaan vauhti ja intensiteetti vaihtelevat suuresti kisan aikana. Suunnistajathan kulkevat perinteisesti ruisleivällä ja kaurapuurolla ja pastalla, mutta jos haluaa noudattaa paleo-tyyppistä ja hiilihydraattitietoista ruokavaliota, eivät nuo ruoka-aineet sovi kuvaan.” - Pietari “Vinkkejä ohjelmointiin kaivataan, ongelmana lienee kiinnostuksenkohteiden paljous. Miten suosittelisitte rakentamaan treenikokonaisuuden intensiteetti/kesto/lepo jne kun tykkään monipuolisesta kestävyysurheilusta Sen lisäksi tykkään myös painonnosto- ja crossfittreenaamisesta. Kuntoa tulee testattua osallistuen monenmoisiin haasteisiin kestoltaan 3-36 h sisältäen joko yksittäisiä lajeja (maastopyöräily, -juoksu, melonta) tai kaikkea yhdessä tapahtumassa (=seikkailu-urheilu). Olen treenannut pidempään aktiivisesti ja ylirasitustilat ovat tuttuja, kun kokonaisuus tuntuu treenien puolesta hajoavan. Tuhoaako vai tukeeko salitreeni ja metconit mahdollisuuteni kestävyyskuntourheilijana? Teen salilla syksyllä 3-4 ja keväällä n. 2 treeniä viikko, lisäksi 3-4 sisällöltään vaihtelevaa lajitreeniä. Kesä on pääasiallinen kisakausi, jolloin teen korkeaintensiiviset treenit lajimuotoisina. Onko 2 crossfittreeniä viikossa turhaa vai auttaako se kehittämään ominaisuuksia?” - Kaisu “Moro, Lepopäivään tuli mieleen tämmöinen kysymysehdotus: Kuinka palata takaisin rankkaan toiminnalliseen harjoitteluun flunssakauden jälkeen, jos ja kun kunnon treenaamiselle tulee viikon parin tauko? Kuinka aloitella siis sykettä nostavia kiertoharjotteluja sekä kuinka palata flunssan katkaisemaan tavoitteelliseen voimaharjoitteluun?” - Markus “Mitä mieltä olette yksin harjoittelusta. Kannattaako painonnostoliikkeitä harjoitella yksin? Jos tavoitteena on hyvä toiminnallinen kunto ja atleettisuus, onko painonnosto (tempaukset ym.) mielestänne hyvä harjoittelumuoto? Kiitos!” - Arska Jakson linkit: 3fu3l http://3fu3l.com/ Hörhö BulletProof-podcastissa http://www.bulletproofexec.com/102-extreme-endurance-training-and-ketosis-with-ben-greenfield-podcast/ Puffit Paleoradio https://soundcloud.com/paleoradio https://www.facebook.com/paleoradiopodcast Sisusavotta http://www.sisusavotta.fi/sisusavotta-podcast-6-jaakko-savolahti/ Toimivan ravintovalmennuksen perusteet 3.5. Olli Sovijärvi ja Sami Sundvik Tiedot js.com ja maaliskuun loppuun asti 10% alea jaskanlista10 kupongilla Urheilijan Ravitsemus -luento CF Keravalla 18.5. http://crossfitkerava.com/2014/03/20/urheilijan-ravitsemus-luento/ Luonnollisen liikkumisen leirit ja kurssit, yksi paikka perusleirille ja alehinta voimassa 4.4. asti.

Antibodies and antibody fragments are essential tools in basic research, diagnostics and therapy. Conventional antibodies consist of two heavy and two light chains with both chains contributing to the antigen-binding site. In addition to these conventional antibodies, camelids (llamas, alpacas, dromedaries and camels) possess so-called heavy chain antibodies (hcAbs) that lack the light chains. The antigen binding site of these unusual antibodies is formed by one single domain only, the so called VHH domain. The VHH domain represents the smallest intact antigen binding fragment (~ 15 kDa) and is characterized by very high stability, solubility and specificity. These unique features render VHHs a promising alternative to conventional antibodies and antibody fragments with a multitude of possible applications. In the course of this thesis, we aimed to generate and characterize VHHs suitable for different applications ranging from biochemical studies to immunofluorescence assays and live cell imaging. In order to meet the diverse requirements of the intended downstream applications, a new selection method differing from traditional phage display, called native panning, was developed and established. In combination with the protein-protein interaction fluorescent two-hybrid (F2H) assay, a new process to identify antigen specific VHHs functional inside living cells was developed. We used the immune system of alpacas to generate VHH libraries against various antigens, ranging from small peptides to large proteins. The libraries were screened by phage display and antigen specific VHHs were identified by phage ELISA. We were able to identify antigen specific VHHs against different antigens which were characterized and their functionality was tested in various applications. Furthermore, we could demonstrate that the selection method influences which VHHs are identified and therefore needs to be chosen very carefully with regard to the intended biochemical and cell biological application. In summary, we developed and established an efficient and versatile process to screen and identify antigen specific VHHs suitable for different downstream applications.