Podcasts about o157

Dr. Don and Professor Ben talk about the risks that Ben faced when eating raw milk gouda cheese in an unregulated Brazilian facility. Dr. Don - risky ☣️ Professor Ben - risky ☣️ Food Safety Talk NC Tobacco Trust Fund Commission Agricultural Leadership Development Program | NC State Extension Gouda Recipe | Cheese Maker Recipes | Cheese Making Supply Enumeration of Escherichia coli O157 in Outbreak-Associated Gouda Cheese Made with Raw Milk - ScienceDirect Outbreak of Escherichia coli O157:H7 Infections Linked to Aged Raw Milk Gouda Cheese, Canada, 2013 - ScienceDirect Multistate Outbreak of Escherichia coli O157:H7 Infections Associated with In-Store Sampling of an Aged Raw-Milk Gouda Cheese, 2010 - ScienceDirect Escherichia coli O121 outbreak associated with raw milk Gouda-like cheese in British Columbia, Canada, 2018 - PMC Microbiological Safety of Various Gouda Cheeses Produced from Raw Milk

Podcast: Bites & Bytes PodcastEpisode: Cybersecurity's Role in Modern Food Defense with Radojka BaryckiPub date: 2024-11-26Get Podcast Transcript →powered by Listen411 - fast audio-to-text and summarizationIn this episode of the Bites and Bytes Podcast, Kristin Demoranville is joined by Radojka Barycki, Director of Training and Consulting at Safe Food Alliance and a food safety expert with nearly 25 years of experience.  Together, they explore how cybersecurity and food safety are becoming inseparable in today's technology-driven world.  Radojka shares her perspectives on emerging challenges like AI and traceability, the role of proactive strategies in protecting global food systems, and why trust is the foundation of food safety.  With stories from her personal journey and actionable insights for industry professionals, this episode sheds light on how innovation can both secure and transform the food industry.  A must-listen for those passionate about food safety, technology, and consumer protection! _______________________________________________ Episode Key Highlights: (0:02:09) - Food Defense and Cybersecurity Collaboration (0:03:20) - Awareness of Cybersecurity Threats in Food (0:05:55) - Personal Risk Assessments and Security Mindsets (0:11:26) - Integrating Cybersecurity in Food Safety Audits (0:13:37) - Vulnerabilities in Automated Food Processes (0:16:17) - Challenges in Traceability and AI Adoption (0:19:00) - Reactive Policies vs. Proactive Prevention in Food Safety (0:22:28) - Impacts of Food Safety Incidents on Consumer Trust (0:27:56) - Resources for Food Defense and Risk Assessment _______________________________________________ Show Notes: Traceability rule:  https://www.qualityassurancemag.com/news/navigating-the-new-fda-food-traceability-rule-meeting-requirements-and-enhancing-safety/ Food Adulteration:  https://www.fda.gov/food/food-safety-modernization-act-fsma/fsma-final-rule-mitigation-strategies-protect-food-against-intentional-adulteration Food Safety Modernization Act (FSMA):  https://www.fda.gov/food/guidance-regulation-food-and-dietary-supplements/food-safety-modernization-act-fsma Food Defense:  https://www.fda.gov/food/food-defense AI for Traceability:  https://www.food-safety.com/articles/9387-development-and-application-of-ai-for-food-processing-and-safety-regulations#:~:text=Current%20AI%20technologies%20can%20be,enhance%20traceability%20for%20food%20safety.&text=This%20could%20facilitate%20a%20quicker,of%20food%20contamination%20or%20recall. Carrot Juice Cotulism Incident 2006: https://www.cidrap.umn.edu/botulism/fourth-botulism-case-linked-carrot-juice Hazard Analysis Critical Control Point (HACCP) & the Seven Principles: https://food.unl.edu/article/haccp-seven-principles Peanut Corporation of America and FSMA: https://www.nejm.org/doi/full/10.1056/NEJMp1109388 Boars Head Incident 2024: https://www.marlerblog.com/case-news/boars-head-listeria-outbreak-deemed-over-19-states-61-sick-60-hospitalized-with-10-dead/ McDonald's Onion E. coli Incident 2024: https://www.cdc.gov/ecoli/outbreaks/e-coli-O157.html#:~:text=Recalled%20food,onions%20before%20they%20got%20sick. Spinach E. coli Outbreak 2006: https://archive.cdc.gov/www_cdc_gov/ecoli/2006/spinach-10-2006.html Tesla products mentioned:  Cybercab, Robovan, Bot https://www.tesla.com/we-robot Satellite made of wood called LignoSat (Japanese): https://physicsworld.com/a/timber-japan-launches-worlds-first-wooden-satellite-into-space/#:~:text=Researchers%20in%20Japan%20have%20launched,the%20logging%20firm%20Sumitomo%20Forestry. FDA: https://www.fda.gov/ Food Defense Plan Builder: https://www.fda.gov/food/food-defense-tools/food-defense-plan-builder Food Defense Mitigation Strategies Database: https://www.hfpappexternal.fda.gov/scripts/fooddefensemitigationstrategies/index.cfm _______________________________________________

Welcome to episode 21 of Growers Daily! We cover: e. Coli in the Onions, Farm Franchises and my Favorite Crops to Harvest  E. Coli outbreak: https://www.cdc.gov/ecoli/outbreaks/e-coli-O157.html   Support our work (

「死者も…マクドナルドでハンバーガー食べた人が相次ぎO157感染　「クォーターパウンダー」のタマネギが感染源　アメリカ」　アメリカで、マクドナルドの一部のハンバーガーを食べた人がO157に相次いで感染しました。CDC（疾病対策センター）の発表によりますと、コロラド州など10の州で、マクドナルドの「クォーターパウンダー」と呼ばれるハンバーガーを食べた人が、相次いで体調不良を訴えました。これまでに49人が腸管出血性大腸菌・O157に感染し、このうち1人が死亡、10人が入院したということです。マクドナルドは声明を発表し、「クォーターパウンダー」に入っていたタマネギが感染源である可能性があるとして、当面の間、感染が確認された州を中心に販売を中止すると発表しました。

「【注意】“飲めるハンバーグ”でO157食中毒34人発症…客が焼くスタイルで加熱不十分か」　千葉・船橋市の「将泰庵DINER」の人気メニュー、A5ランクの黒毛和牛を使った「飲めるハンバーグ」。8月24日から28日にかけて、このハンバーグを食べた人たちが下痢や腹痛などの症状を訴えました。船橋市保健所は、調査により腸管出血性大腸菌「O157」が原因の食中毒と断定。発症者は、これまでに34人に上っています。保健所によると、入荷した時点で肉が汚染されていた可能性があるとしていて、調査中だといいます。客が鉄板で焼き加減を調整できる飲めるハンバーグ。実際に食べた人が撮影した映像をよく見ると、中心部分はまだ真っ赤な状態です。5月に飲めるハンバーグを実際に食べた人に、どのような食べ方だったかを聞くと「鉄板で焼いてから食べるように言われていて、そういうシステム。よく焼いて赤身をなくして食べるというふうに」と話します。O157は75度で1分以上加熱し中まで火を通すことで死滅しますが、今回は加熱が不十分だったため食中毒が起きたといいます。ではハンバーグを調理する際に気を付けるポイントは？「イット！」取材班は、ランチでにぎわう東京都内のレストランへ向かいました。ハンバーグレストランまつもと・芦澤冬樹料理長：この時期だと調理場も暑いので、できるだけ菌を増やさないように。こちらの店では、まずフライパンで約4分間表面にしっかり焼き目をつけ、オーブンで約12分加熱し中までしっかり火を通したうえで客に提供しているといいます。芦澤冬樹料理長によると、「最低でも赤い色がなくなるくらいまでは焼いた方がいい」といいます。さらに家庭では「（フライパンに）ふたをして蒸す感じにしてというのなら家庭でもできると思うんですけども、オーブンがあるところは、使った方が確実に火が入る」と話します。専門家によると、まだ暑い日が続く今の時期、調理には特に注意が必要だとしています。食環境衛生研究所・小林幸嗣さん：基本的に何らかの食中毒菌はいるものとして、加熱とか丁寧に取り扱うというのが大切。

Dr. Don and Professor Ben talk about the risks from making a sandwich with “help” from Bruce the Cow. Dr. Don - risky ☣️ Professor Ben - risky ☣️ Bruce the cow loves sandwiches (and I love Bruce) : r/StupidFood Does the maternal grooming of cattle clean bacteria from the coat of calves? - ScienceDirect Cattle Water Troughs as Reservoirs of Escherichia coli O157 - PMC Escherichia coli O157:H7 in Environments of Culture-Positive Cattle - PMC An investigation of Escherichia coli O157 contamination of cattle during slaughter at an abattoir - PubMed Reducing the Risks of Pathogenic E. Coli Infections Trying to eat breakfast with Bruce! #cow #brucethecow #food #snack #fi… | cows | TikTok

Vanessa Coffman, Ph.D. is the Director of the Alliance to Stop Foodborne Illness. She has a diverse background in food safety and sustainability, with a focus on environmental exposures across the food system. Dr. Coffman has conducted various research for the Food and Agricultural Organization of the United Nations (FAO), on topics such as farming opportunities in post-war Sierra Leonne, occupational and residential exposures from large pork production operations in rural North Carolina, and the association between nitrate in drinking water from food animal operations and fetal health outcomes. Dr. Coffman previously worked at Stop Foodborne Illness as a policy analyst, and she has testified in front of U.S. government officials, authored peer-reviewed papers, and helped draft federal regulations. Dr. Coffman received a Ph.D. in Environmental Epidemiology from the Johns Hopkins Bloomberg School of Public Health and an M.S. degree from the University of California–Berkeley in Global Public Health and the Environment. Sherry Brice-Williamson, M.B.A. is the Vice President of Global Quality and Food Safety at the Kellogg Company, where she oversees end-to-end food safety and quality for Kellogg's internal and external network. Sherry has over 20 years of experience in the industry and joined Kellogg in 2012 as part of the Pringles acquisition from P&G. She has served in numerous supply chain roles in the company, ranging from operations to quality. Sherry was promoted to Vice President of Global Food Safety and Quality in January 2020. Sherry is on the SSAFE board of directors and is affiliated with a number of other industry associations such as Stop Foodborne Illness, where she is an Alliance member. Sherry also co-chairs the national chapter of KAARG (Kellogg African American Resource Group). Sherry holds B.S. and M.B.A. degrees in Business Management and is a member of the Golden Key International National Honors Society. Megan Kenjora, M.P.A. is the Senior Manager of Food Safety Culture at The Hershey Company, where she leads a diverse global team to embed food safety in the hearts and minds of all Hershey employees. Megan has extensive experience building relationships among diverse groups, getting cross-functional support, and effectively communicating messages across cultures. Megan was an engaged member of the Global Food Safety Initiative (GFSI) Technical Working Group that authored the GFSI position paper, “A Culture of Food Safety,” and served as the inaugural chair of the International Association for Food Protection (IAFP) Food Safety Culture Professional Development Group. Bringing a passion for food safety culture, she currently serves on the planning committee and numerous working groups as part of the Alliance to Stop Foodborne Illness. A combat veteran who served eight years in the U.S. Army, Megan came to Hershey in 2014 from Raytheon, where she specialized in adult learning for various intelligence courses. She is a lifelong learner and an advocate for DEI, serving as a co-lead for the Hershey Veterans Business Resource Group. Megan is an M.B.A. candidate at UNC Kenan-Flagler Business School, and she holds an M.P.A. from Penn State University, B.A. degrees in Political Science and Classics from Bucknell University, and a Korean linguist certification from the Defense Language Institute. In this episode of Food Safety Matters, we speak with Vanessa, Sherry, and Megan [25:40] about: The history of Stop Foodborne Illness and the Alliance to Stop Foodborne Illness, and how the Alliance leverages food safety culture as a vehicle for positive change in the food industry to make food safer for consumers The ways in which Megan's experience at Hershey informs the Alliance's work, such as bringing a proactive perspective to industry engagement and encouraging the adoption of best practices How Kellogg's and Hershey's memberships in the Alliance have helped both companies drive sustainable food safety cultures through collaboration and knowledge-sharing The resources included in Stop Foodborne Illness' Food Safety Culture Toolkit, which is tailored for small- and medium-sized businesses How the Alliance's work, such as advocacy for recall modernization, is steered by the needs and expertise of its members Why it is important for industry to understand the crucial need for food safety, and how resources such as video messaging and gamification frameworks can support that understanding The importance of clear and effective food safety communication from upper management, and how the Food Safety Culture Toolkit aids this objective The ways in which Sherry and Megan have established successful communication practices within their organizations to encourage robust food safety cultures. News and Resources Poisoned—Book on Prominent Foodborne Illness Outbreak—Getting New Edition, Netflix Documentary [2:08] FDA Submits FSMA Traceability Final Rule Ahead of New Era of Smarter Food Safety Webinar [7:26] 2020 Estimates Released: Food Types Associated with E. Coli, Salmonella, Listeria Foodborne Illness Outbreaks [14:46] Trust, Transparency Focus of Maple Leaf Foods' 12th Annual Food Safety Symposium [20:12] Ep. 128. Bill Marler: Perspectives on Poisoned and Food Safety Progress FSMA Proposed Rule for Food Traceability (FDA) Foodborne Illness Source Attribution Estimates for 2020 for Salmonella, Escherichia coli O157, and Listeria monocytogenes using Multi-Year Outbreak Surveillance Data, United States (CDC) We Want to Hear from You! Please send us your questions and suggestions to podcast@food-safety.com

「ユッケ食べ女性死亡　食中毒で...O157検出」　京都府の精肉店で買ったユッケを食べた90代の女性が、腸管出血性大腸菌・O157による食中毒で死亡した。90代の女性は8月27日、京都・宇治市の精肉店「Meat & Fresh TAKAMI」でユッケを買い、自宅で食べたところ、6日後に腹痛の症状が出た。女性からはO157が検出され、15日に死亡した。京都府によると8月22日から26日の間に、この店のユッケや「ローストビーフ」を食べた9歳から87歳の22人が食中毒の症状を訴えているという。京都府は、この店を9月6日から営業停止処分にしたという。加熱などの処理が不十分だった可能性があるということで、京都府は中心部まで十分に加熱して食べるよう呼びかけている。

Join Jame and Callum and they discuss some of the other Enterobacterales Gram negatives. Particularly breaking down them into their families and into Lactose fermenters and non-fermenters. Jame has a small breakdown around our Twitter handle near the end.Comments suggestions and criticisms to idiotspodcasting@gmail.com or @IDiots_pod Some lists:Chromosomal AmpC Carriers (high-risk): HECK YES·         Hafnia alvei, Enterobacter cloacae, Citrobacter freundii, Klebsiella aerogenes, Yersinia enterocoliticaLower Risk Chromosomal AMPC: Vulgar Providencia Store AMPC·         Proteus Vulgaris, Providencia, Serratia, Acinetobacter, Morganella, Providencia Intrinsic Colistin Resistance: ·         Burkholderia cepacia (Nonfermenter), Proteus, Providencia, Serratia Biochemical mimics of Salmonella (also can group)·         Hafnia, Morganella,Proteus Cat 3 organisms: ·         Salmonella typhi/paratyphi, Shigella dysenteriae type 1, E.coli O157, Yersinia PestisSome useful resources:SMI ID 16: identification of Enterobacteriaceaehttps://www.gov.uk/government/publications/smi-id-16-identification-of-enterobacteriaceae Janda JM, Abbott SL. 2021. The changing face of the family Enterobacteriaceae (order: “Enterobacterales”): new members, taxonomic issues, geographic expansion, and new diseases and disease syndromes. Clin Microbiol Rev 34:e00174-20. https://doi.org/10.1128/CMR.00174-20 

Cathal Friel, Co-Founder & Chairman & Jeremy Skillington, CEO of Poolbeg Pharma #POLB discuss the Option Agreement they signed with University College Dublin for a Melioidosis Vaccine candidate, MelioVac, and a licence to evaluate 5 other infectious disease portfolio assets. Highlights Poolbeg Pharma , a clinical stage infectious disease pharmaceutical company with a capital light clinical model, has signed an Option Agreement to licence MelioVac, a vaccine for melioidosis, with University College Dublin ('UCD') and its inventor, Associate Professor Siobhán McClean, through NovaUCD, the university's knowledge transfer office.  The Company will continue its due diligence on MelioVac, a preclinical asset and recipient of a Wellcome Trust Award to aid its development, as well as 5 of other potential vaccine candidates discovered by Associate Professor McClean and her team, for the duration of the Option Agreement, prior to signing a 'Licence Agreement'.  Dr McClean is Associate Professor and Head of Biochemistry at the UCD School of Biomolecular and Biomedical Science. Dr McClean completed her BSc in Biochemistry in UCD and received her PhD from Imperial College London. Her research focuses on lung infections which led her to develop a platform technology to identify proteins that bacteria use to attach to human cells. These proteins have proved to be excellent vaccine candidates. Dr McClean completed some of the original research to identify the antigens associated with the Melioidosis Vaccine at TU Dublin. Poolbeg Pharma has identified melioidosis as an infectious disease of interest due to its rising incidence around the world and because there is currently no approved vaccine available. Concerns are growing about global warming contributing to the spread of the disease to traditionally non-tropical areas. Melioidosis, also known as Whitmore's disease, is an infectious disease caused by the bacterium Burkholderia pseudomallei, commonly found in the soil and surface groundwater of many tropical and subtropical regions, with diverse clinical presentations including pneumonia and severe sepsis with multiple organ abscesses. Incidence of the disease is widespread in South-East Asia, Northern Australia and India, with climate change having a substantial impact on the spread of the disease to new areas such as Brazil. There are an estimated 165,000 cases of melioidosis each year, of which as many as 89,000 (54%) are estimated to be fatal.  Other potential vaccine candidates that the Company is evaluating include those for Klebsiella pneumoniae, Escherichia coli (O157), Burkholderia cepacia complex, Pseudomonas aeruginosa and Acinetobacter baumannii. About Poolbeg Pharma Poolbeg Pharma is a clinical stage infectious disease pharmaceutical company, with a capital light clinical model which aims to develop multiple products faster and more cost effectively than the conventional biotech model. The Company, headquartered in London, is led by a team with a track record of creation and delivery of shareholder value and aspires to become a "one-stop shop" for Big Pharma seeking mid-stage products to licence or acquire. The Company is targeting the growing infectious disease market. In the wake of the COVID-19 pandemic, infectious disease has become one of the fastest growing pharma markets and is expected to exceed $250bn by 2025. With its initial assets from Open Orphan plc, an industry leading infectious disease and human challenge trials business, Poolbeg has access to knowledge, experience, and clinical data from over 20 years of human challenge trials. The Company is using these insights to acquire new assets as well as reposition clinical stage products, reducing spend and risk. It already has a Phase II ready repositioned small molecule immunomodulator for severe influenza and a portfolio of other exciting assets. The Company plans to broaden this portfolio further going forward and is in active discussions with AI data analysis platforms to help accelerate the power of its human challenge model data and biobank.

Dr. Don and Professor Ben talk about the risks of eating dirty raw fresh mushrooms. Dr. Don - risky ☣️ Professor Ben - risky ☣️ Incidence of Enterohemorrhagic Escherichia coli, Escherichia coli O157, Salmonella, and Listeria monocytogenes in Retail Fresh Ground Beef, Sprouts, and Mushrooms | Journal of Food Protection | Allen Press Evaluation of Microbial Loads on Dried and Fresh Shiitake Mushrooms (Lentinula edodes) as Obtained from Internet and Local Retail Markets, Respectively Outbreak of Listeria Infections Linked to Enoki Mushrooms | Outbreak of Listeria Infections Linked to Enoki Mushrooms | March 2020 | Listeria | CDC Cultivable microbiome of fresh white button mushrooms - Rossouw - 2017 - Letters in Applied Microbiology - Wiley Online Library Microbial ecology of mushroom casing soils and preharvest strategies to enhance safety and quality of fresh mushrooms

Dr. Don and Professor Ben talk about the risks of eating dirty raw fresh mushrooms.Dr. Don - risky ☣️ Professor Ben - risky ☣️ Incidence of Enterohemorrhagic Escherichia coli, Escherichia coli O157, Salmonella, and Listeria monocytogenes in Retail Fresh Ground Beef, Sprouts, and Mushrooms | Journal of Food Protection | Allen Press Evaluation of Microbial Loads on Dried and Fresh Shiitake Mushrooms (Lentinula edodes) as Obtained from Internet and Local Retail Markets, Respectively Outbreak of Listeria Infections Linked to Enoki Mushrooms | Outbreak of Listeria Infections Linked to Enoki Mushrooms | March 2020 | Listeria | CDC Cultivable microbiome of fresh white button mushrooms - Rossouw - 2017 - Letters in Applied Microbiology - Wiley Online Library Microbial ecology of mushroom casing soils and preharvest strategies to enhance safety and quality of fresh mushrooms

今年は音声入力にスイッチして、音でも聴くことのできる、完全口語体のブログに変えましたが、このやり方は来年も続けていこうと思っています。気がつけば今年も残りあと10日、毎日ひと月分の過去ブログを整理してきて、今日は今年の4月をやったのですが、あれ、この日記書いたの、こんな前だったのかと驚くことばかり。本当に、昨日のことのようです。つぎは今年5月30日の徒手空拳日記です。引用します。 インドのアビギャ・アナンドという占い師が、昨年8月時点のYoutubeですでに、かなり正確に今回のパンデミックを言い当てていたことが、いま注目されています。彼の予言はここでは終わっておらず、今年の年末には、さらに厄介なウイルスのパンデミックが起きると予言されています。（引用、終わり） 最近またこのインドの14歳の少年の予言が、話題になっています。今年前半の世界的なコロナ禍の猛威を的中させていたのですが、この年末についての彼の予言も、なんだかいやーな一致をし始めているというもので、今日12月20日から来年の3月まで、より致死率の高いスーパーバグが現れるという予言です。 南アフリカ共和国やイギリスでは、感染力が強くなった変異ウイルスが発見されていますが、これがスーパーバグなのかどうかは、わかりません。イギリスや南アフリカの話だから、他人事なのではなく、日本でもローカルな突然変異は、当然起こりうるので、心配ですね。 最悪のケースを想定して、対応することが大切ではないかと思います。コロナ禍に限らず、私は仕事でもなんでも、わざと最悪の事態を想定してみることにしています。考えられるなかで最も最悪の事態です。コロナ禍で言えば、リアルな医療崩壊、親の感染、自分の感染、株価の暴落、企業の連鎖倒産、預金封鎖、ハイパーインフレなどでしょうか。これよりもさらに、意表をつく最悪の事態を考えるのです。例えば、インターネットの長期に及ぶ完全停止があるかもとか、ワクチンそのものが実は、感染するスーパーバグになるかもとか、そういった頭の体操です。 世田谷区の看護師や介護福祉士への積極的検査の結果を参考にすると、おそらくいま現在の都内の陽性率は、1から2%のあいだだろうと思います。100人にひとりからふたりの間、結構高い確率です。インフルエンザやO157の感染者は激減しているのに、このコロナの高い感染率は、やはり無症状の陽性者が広げてしまっているのでしょう。 いつも書いてますがこの国では、政財官学マスメディアの言ってることより、在日米軍がなにを考えているか、しているかが大切です。例えば12月3日から、在日米軍は都内から150キロメートル圏内の飲食店は利用禁止らしいです。つまり、関東平野丸っと、米軍は外食禁止みたいです。六本木とか横須賀とか、閑古鳥だと思います。 それに比べて政財官学マスメディアは、食べたり飲んだりするときはマスクを外し、それ以外のときはマスクを着用する、マスク会食なる、お歯黒を越える奇行を国民に奨励しており、真正キチガイではないかと思います。欧米のように、飲食店の売上を一定額補償して、期間を決めて一斉閉店すべきです。 萩生田文科相が、芸者付きマスクなしで4人以上の会食をしていたとか、最近はマスク会食警察とも言うべきチクリが跳梁跋扈しています。いまの内閣はガチの警察、公安内閣と言われていますが、マスク警察、自粛警察、マスク会食警察のような、ネットワークによる相互監視、パノプティコンのカギカッコ付きの「警察」のほうが、ヒエラルキーによる警察よりも、権力を持ち始めているのは、象徴的な権力の終焉、だなと思います。

Les artistes - comédien, chanteur, danseur, metteur en scène - d'Alsace et d'ailleurs partagent leurs expériences d'artistes, leurs doutes, leurs joies et leurs victoires. Nous les remercions chaleureusement pour leur participation. David Gies est comédien et travaille également à la mise en scène. Il est professeur de théâtre, donne des cours d'improvisation et de coaching vocal en Alsace.

Over 25-years ago, an outbreak of E coli O157 in Jack in the Box restaurants in the States made people wake up to the fact that their hamburger could kill them. It would seem that the innocence, the fun, the love, American consumers had with burgers disappeared as newspapers became filled with stories about the...

Na trilogia “De Volta para o Futuro” vemos o Dr. Emmet Brown usando resíduos orgânicos que pega do lixo para abastecer o DeLorean e ir com o Marty e Jennifer para o futuro. Tirando a parte de viajar no espaço-tempo, utilizar material orgânico para produzir biocombustíveis já é realidade. No episódio #6 do Podcast Microbiando discutimos uma alternativa para a produção de biocombustível a partir de fermentação de lixo orgânico. O texto em discussão foi publicado em 2018 por um grupo da Universidade Nacional da Irlanda em Galway e se chama “Reproducible, high-yielding, biological caproate production from food waste using a single phase anaerobic reactor system” e em português “Produção biológica, reprodutível e de alto rendimento de caproato a partir de dejetos alimentícios usando um reator anaeróbico de fase simples (ou única)”. No Microlitros de Notícias, trouxemos diversas matérias escritas pelos nossos alunos de graduação e pós-graduação. Juliana Guimarães trouxe uma reflexão sobre a população bacteriana da microbiota intestinal de ursos pardos durante a hibernação; Gustavo Meira revela a relação entre o tipo sanguíneo e a predisposição em desenvolver sintomas graves de uma infecção bacteriana; Cecília Vieira fala sobre a descoberta de células linfóides inatas (ILCs) no leite materno e como isso ajuda a proteção dos bebês. No Filogenia da Ciência, conhecemos mais sobre a vida acadêmica do pesquisador Alexander Fleming, descobridor do antibiótico penicilina.   Tópicos comentados nesse episódio Fermentação Reator anaeróbico Microrganismos anaeróbicos Lixo orgânico Biocombustível Caproato Carboxilato/Ácidos orgânicos Síntese de ácidos graxos Fermentador do tipo leach bed reactor (reator de leito de lixiviação) Leite materno Alexander Fleming Penicilina   Referências desse episódio 2018.  Nzeteul, C. O., Trego, A. C., Abram, F., e O’Flaherty V. Reproducible, high-yielding, biological caproate production from food waste using a single-phase anaerobic reactor system. Biotechnol Biofuels. 2002. Blackwell, C. et al. Blood Group and Susceptibility to Disease Caused by Escherichia coli O157. The Journal of Infectious Diseases. 2006. Fleckenstein, JM. Identification of a two-partner secretion locus of enterotoxigenic Escherichia coli. Infection and Immunity. 2018. Kumar, P. et al. Enterotoxigenic Escherichia coli blood group A interactions intensify diarrheal severity. The Journal of Clinical Investigation. 1993. Navas, E. et al. Blood group antigen expression on vaginal cells and mucus in women with and without a history of urinary tract infections. The Journal of Urology. 2016. Sommer F., et al. The Gut Microbiota Modulates Energy Metabolism in the Hibernating Brown Bear Ursus arctos. Cell Reports. 2015. Tan, S. Y. e Tatsumura, Y. Alexander Fleming (1881–1955): discoverer of penicillin. Singapore medical journal. 2016. Dilva Frazão. Biografia de Alexander Fleming. eBiografia. 1945. Alexander Fleming. Nobel Lecture. Nobel Prize 2014. Nobel Media AB. Sir Alexander Fleming - Biographical 2018. Sascha Cording, Jasna Medvedovic, Emelyne Lecuyer, Tegest Aychek, Gérard Eberl. Control of pathogens and microbiota by innate lymphoid cells. Microbes and Infection. 2018.Baban, Malik, Bhatia, Yu J. C. Presence and Profile of Innate Lymphoid Cells in Human Breast Milk. JAMA Pediatri.   Sobre o Podcast Microbiando A ideia do Microbiando é discutir artigos científicos de ponta em todas as áreas da microbiologia e imunologia. Vamos utilizar uma linguagem bem acessível para destrinchar esses artigos para vocês, mas sem perder o rigor científico e analítico necessário para essa tarefa. Além de discutir artigos nós teremos o quadro Microlitros de Notícias, onde nossos microbiologistas e imunologistas de plantão irão abordar pequenas reportagens e trazer novidades para vocês. No quadro filogenia da Ciência vamos contar um pouco sobre a vida de grandes personalidades que revolucionaram a Mic...

Bomb Cyclone Blizzard: Uber-Like Snow Shovel Apps are Here to Help Raleigh breaks record for most consecutive hours of freezing temps Shinny - Wikipedia Lindsay Christians (@LindsayC608) | Twitter Edible vs. Non-Toxic: The Great Glitter Debate Daily Coffee News by Roast Magazine Nail art fans use science skills to research diamond cappuccino Glittery coffee trend Diamond and Gold Cappuccino Food Additives & Ingredients > FDA Advises Home and Commercial Bakers to Avoid Use of Non-Edible Food Decorative Products The $666 gold-wrapped Douche Burger: Is it safe to eat gold? Stooblert’s message made be think of John Gruber & Merlin Mann’s Blogging Panel at SxSW What Foods Are Most Likely to Make You Sick? Smithsonian 58 sick, 2 dead, possible link to romaine lettuce | barfblog 21 sick with E. coli O157 from Romaine lettuce in Canada: What says LGMA? | barfblog CDC Investigating Multistate Outbreak of E. coli O157:H7 Infections Lettuce suspected in deadly US E. coli outbreak - CNN Pulsed-field Gel Electrophoresis (PFGE) Largest food service company in U.S. suspends use of romaine lettuce amid E. coli scare - Chicago Tribune Food Safety Talk 142 Joint statement on E. coli Investigation - LGMA Silicon Valley’s ‘raw water’ trend could turn deadly - Business Insider Unfiltered Fervor: The Rush to Get Off the Water Grid - The New York Times Your frozen ready-to-bake biscuits might have Listeria in/on them | barfblog T. Marzetti Company Voluntarily Recalls Frozen Biscuit Dough Packed Under Various Brands Due to Don Schaffner ☣️ on Twitter: “So today pathogens spontaneously form in our leftovers? That’s super cool! https://t.co/V4FihrN8rP” One Mississippi (TV Series 2015– ) - IMDb The Marvelous Mrs. Maisel (TV Series 2017– ) - IMDb Netflix Doc Series ‘Rotten’, ‘Dirty Money’ Tackle Corruption

Initial detection and reporting by clinical microbiology laboratories is a sentinel marker for foodborne outbreak surveillance systems. Initiation of a public health investigation is reliant on the rapid initial identification of pathogens of interest. Diagnostics for Escherichia coli have evolved to reduce identification turnaround time, incorporating technologies for rapid identification (MALDI-TOF MS) and serogrouping (O157 antiserum or latex agglutination). Reporting of these isolates to a public health agency may initiate further laboratory investigations, such as pulsed-field gel electrophoresis, for confirmation that isolates may be related to a common source (clonal population).

The guys started the show dreaming about a Red Mac Pro. They then turned to the passing of Bill Keene. Bill has been mentioned in various FST episodes and was a well respected epidemiologist as seen in the articles by the Oregonian and Doug Powell. The guys then turned to their beverages, Coffee Club, Napoleon Dynamite, Homeland, and Car Talk. Ben shared his preference for Aussie Rule football and Arcade Fire's album Reflektor. The conversation then turned to Don's limited iPhone music library, Privateering and Dire Straights, which reminded Ben of Money for Nothing and WWE Wrestling (not WWF Wrestling). To finish they talked about Christmas music, Bad Religion's Christmas Songs, Coulton and Roderick's One Christmas at a Time and Horrible Christmas songs. Ben confused IAFP's History with Bug Trivia and shared Julian Cox's information about the 1960's, and this evolved into a broader discussion about the IAFP and its membership. The discussion then turned back to Bill Keene and some of the outbreaks he had been involved in. This included a Salmonella Panama outbreak (not to be confused with Van Halen's Panama), which was the first outbreak that was solved through the innovative use of supermarket loyalty cards and that Bill and others were sued for (the lawsuit was eventually dropped.. The guys then discussed outbreak investigation in some detail and that public health officials are damned if they do and damned if they don't name commodities and suppliers. There is of course always a risk of getting the epidemiology wrong, as was the case with Salmonella Saintpaul in peppers. Finally, Bill's investigation of a Norovirus outbreak reminded Ben of a recent Norovirus outbreak in Las Vegas. Then Ben commented on an exchange with Chris Gunter, who was presenting on traceability for small producers at the 2013 Strawberry Expo. Chris' presentation is based on the investigation of an E. coli O157 outbreak related to strawberries, in which Bill Keene played a part. In the after dark, the guys reflected on mortality and that we should all Enjoy Every Sandwich. And because they love him, Rob Ford got a mention again and again.

Enterohämorrhagische E. coli (EHEC) gehören zu den wichtigen, lebensmittelassoziierten Erregern von Gastroenteritiden. Schwere Erkrankungsverläufe, wie die postinfektiöse Komplikation HUS sind bekannt und betreffen meist Kinder unter 5 Lebensjahren. Als Primärquelle gelten Wiederkäuer, in deren Gastrointestinaltrakt das natürliche Habitat von Shigatoxin-bildenden E. coli liegt. Über fäkale Kontamination von Lebensmitteln, Wasser oder auch direkten Kontakt können STEC oral vom Menschen aufgenommen werden, wobei nicht alle STEC gleich virulent sind. Manche, wie z. B. die der Serogruppen O26, O103, O111, O145 und O157 werden wesentlich häufiger bei erkrankten Menschen nachgewiesen als andere. Ziel dieser Studie war es zum einen fünf Singleplex Real-Time PCR-Systeme und ein Pentaplex Real-Time PCR-System zum Nachweis der oben genannten fünf E. coli-Serogruppen am LGL, Oberschleißheim, zu etablieren. Die in der Norm ISO/TS 13136:2012 als „hochpathogen“ eingestuften STEC-Stämme können somit anhand der dort beschriebenen Primer- und Sondensequenzen aus Probenisolaten detektiert werden. Für die Validierung wurden die Selektivität, Sensitivität, Präzision und Richtigkeit der PCR-Systeme bestimmt. Zum anderen wurden die Daten von 8272 humanen Proben (einschließlich der des EHEC O104:H4-Ausbruchs von 2011), 1521 Lebensmittelproben, 240 Tierkotproben, 69 Schlachtkörperproben und 29 Wasserproben aus den Jahren 2009 bis 2011 aus Bayern sowie die STEC-Serotypisierungsergebnisse von 09/2004 bis 12/2011 ausgewertet und beschrieben. Im Vergleich mit der gängigen Serotypisierung mittels Agglutination bietet das Real-Time PCR-Verfahren insbesondere bei großem Probenumfang einen enormen Zeitvorteil. Das Pentaplex PCR-System ermöglicht zudem eine zeitgleiche Analyse von Probenisolaten auf alle fünf Serogruppen. Alle E. coli-Stämme der Serogruppen O26, O103, O111, O145 und O157 wurden durch die PCR-Systeme korrekt nachgewiesen. Die O103-Sondensequenz wurde hierfür zuvor modifiziert. Die Spezifität der Nachweissysteme für O26, O103 und O145 lag in Bezug auf E. coli bei 100 %. Bei den Nachweissystemen für O111 und O157 zeigten auch Bakterienstämme anderer Gattungen ein positives Ergebnis in der PCR (Serratia entomophila / rfbEO157-positiv und Shigella sp. LGL 2869 / wbd1O111-positiv). Die Datenauswertung ergab unter anderem einen hohen Anteil stx-positiver Tierkot- und Schlachtkörperproben von Rindern. Die meisten stx-positiven Lebensmittel stammten von Wiederkäuern. Vereinzelt waren auch potenziell humanvirulente STEC nachzuweisen. Der Großteil der nicht-humanen Proben war weder eae- noch EhlyA-positiv, während nur ein Viertel der humanen Proben keines der beiden Gene aufwies. Bei humanen Proben dominiert die Gruppe der unter 1 bis 5-jährigen Erkrankten und Frauen sind häufiger betroffen als Männer. Die E. coli-Serogruppen O26, O103, O111, O145 und O157 gehören zu den häufigsten in Bayern und 2011 infizierten sich 47 Menschen in Bayern mit EHEC O104:H4.

E. coli O157-Stämme gelten als Prototyp der EHEC, die weltweit beim Menschen verschiedene, teils schwerwiegende Krankheitsbilder wie die hämorrhagische Colitis (HC) und das hämolytisch-urämische Syndrom (HUS) hervorrufen können. Die Produktion von Shigatoxinen, syn. Verotoxinen gilt als primärer Virulenzfaktor dieser Erregergruppe. Zum Reservoir der shigatoxinbildenden E. coli zählen insbesondere große und kleine Wiederkäuer. Mit dem Singlepath® E. coli O157 und dem Duopath® Verotoxins der Fa. Merck stehen zwei GLISA-Schnelltests zur Verfügung, deren Handhabung im Zusammenhang mit der vorliegenden Prävalenzstudie beurteilt wurde.

Bislang wurde angenommen, dass Shiga Toxin 1 (Stx1) im Gegensatz zu Stx2, von dem zahlreiche Varianten existieren, relativ homogen ist. Es waren nur wenige Stx1-Varianten bekannt und diese wiesen mit einer Ausnahme eine Homologie von 99 % mit dem Prototyp aus Phage 933J auf. Neuere Untersuchungen zeigten jedoch, dass auch von Stx1 mehrere Varianten existieren (Stx1c bzw. Stx1OX3, Stx1d und Stx1v52), die deutlich vom Prototyp abweichen (92-97 % Homologie der Aminosäuresequenzen). In der vorliegenden Arbeit wurde das Vorkommen von Stx1-Varianten bei klinisch unauffälligen Rindern, einem wichtigen Reservoir für STEC, untersucht. Dazu wurden 247 Rinderkotproben aus 38 südbayerischen Betrieben nach einer Anreicherungskultur mit PCR auf das Vorhandensein von stx-Genen untersucht und positive Proben mit spezifischen Primern für die unterschiedlichen stx-Suptypen bzw. stx1-Varianten typisiert. Die Isolierung der Keime erfolgte durch Kolonieblothybridisierung. Von den 247 Fäzesproben waren in 124 (50,2 %) Shigatoxin-Gene nachweisbar. Bei der Feindifferenzierung der positiven Proben wiesen 33 nur stx1, 31 nur stx2 und 60 beide Subtypen auf. Von den 93 stx1-positiven Proben enthielten jeweils 16 die Variante stx1c bzw. stx1d. Von den 35 isolierten STEC wiesen sechs, von welchen fünf zu typischen EHEC-Serogruppen (O26, O103 und O157) gehörten, das eae-Gen auf. Ein stx1-, stx2-, eae- und EHEC-hly-positiver O157:H7 sowie ein Sorbit fermentierender O157:H- wurden isoliert. Bei 23 Isolaten konnte EHEC-hly nachgewiesen werden. Vier Isolate mit drei verschiedenen Serotypen (O76:H19, O113:H4, O163:H12 [n=2]) wiesen die Variante stx1c auf, wobei zwei dieser Isolate zusätzlich ein stx2-Gen und das EHEC-hly besaßen. Bei den sechs stx1d-positiven STEC, die den Serogruppen O11:H48, O141:H19, On.t.:H12, On.t.:H48 und Osp:H48 [n=2] angehörten, konnten keine weiteren Virulenzfaktoren nachgewiesen werden. Die Ergebnisse bestätigen die hohe Prävalenz von STEC beim Rind. Außerdem konnte belegt werden, dass die stx1-Varianten stx1c und stx1d mit einer Häufigkeit von je 6,5 % in einem beachtlichen Anteil der 247 Kotproben vorhanden waren. Das Auftreten von stx1c konnte dabei nicht in direkten Bezug mit dem Kontakt zu Schafen gebracht werden. Da die stx1d-positiven Isolate keine anderen Virulenzfaktoren besaßen, dürfte ihre Pathogenität eher niedrig sein.