Podcasts about lgl

premiera: LGL, 6. februar 2025 Radio Slovenija, 7. februar 2025, info oddaje V Lutkovnem gledališču Ljubljana so sinoči uprizorili senčno predstavo z naslovom Mali nauki o čudenju, ki temelji na sporočilih ameriške biologinje in okoljevarstvenice Rachel Carson. Iz njene dokumentarno pisne zapuščine sta lutkovnem predstavo za odraščajočo publiko zasnovala Enrica Carini in režiser Fabrizio Montecchi. Ogledala si jo je Magda Tušar:

Hongi. Update: Experience with me my personal vinyl rip of Squier, Billy - Signs of Life (1984), A-Side. This episode is remastered for playback with headphones and was initially published with mastering for speakers on LGL. . This is the first podcast for Anchor from my existing playlist "One Side At A Time".  Here you find full length lp sides from all music genres with my lovely mumbu-jumbo comments. So the listener can understand how each song is connected (mix, production, song, concept, tension) and engineered to become one lp side. Excuse my german in the beginning of this podcast. I was a bit overwhelmed how amazing this album sounded O.o. Stay

We are not alone! Before we let go of Lost, we want to bring in two people who we got really close to over the years. This is the place we made together so we could find each other. Featuring a final(?) appearance from Producer Kate, Zane and Lauren sit down with Mel and Leah and talk everything Lost, LGL, and their lives! All this and more in this week's Still Lost! Support Us: Venmo: @Zane-Kohler-1 Follow us on all our socials!!  ⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠Tiktok⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠ | ⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠Instagram⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠ | ⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠Twitter⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠ | ⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠Facebook⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠ | ⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠Youtube⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠ |

Do you use auto gain? Autogain is an extremly useful tool in modern music production/playback and should never be dismissed without careful consideration. This FM represents my subjective opinion at the time and should be taken with a grain of salt. Nonetheless, autogain is frowned upon when i play or record music until i find a compelling reason to use it. Playing along is a favorite Charlie Haden album of mine that still needs to be fully appreciated on LGL. Find more Audio/Hi-Fi related nonsense here: patreon.com/Lets_Get_Lost Spontaneous impressions under the influence in meditation posture. Stay

Loosey Goosey Lucifer, the Goddess of Light, who rides a magic, singing fly came knocking on my door. This is the second song in the next LGL album. I don't know at what pace I'll do this album - I'll have to see how quickly the songs want to come to me. The last Loosey Goosey Lucifer album came to me at lightning speed. I have a feeling this one might be slower. Like a slow, steady simmer, instead of an intense boil.Anyway, her friends, the people who belong to this hippie-church, they see her looking sad, and yet shining brighter than usual, at the same time. A car's headlights look bright at night, but not so bright in the daylight. Are we heading into darkness? Is she the headlight that seems brighter all of a sudden cuz the rest of the world is dark? Stay tuned.

Lín-Hó. Fusing Rock and Jazz with plenty of eco-anxiety to maintain reason and compassion in the modern world. LGL will be very soon available in my native tongue on patreon.com/Lets_Get_Lost. Kinda like an alternate reality version of my content in german. Release details under: https://www.discogs.com/release/9853464-Phantoms-Opera-Act-IV https://www.discogs.com/release/3336726-Steve-Tibbetts-Exploded-View Real time impressions under the influence in meditation posture. Stay Love. Patreon:       https://www.patreon.com/Lets_Get_Lost Amazon: https://music.amazon.de/podcasts/57cd4c31-1730-4535-99b8-6b1d219a49c4/LETS-GET-LOST Audible: https://www.audible.com/pd/Podcast/ B08K573BJ9 TuneIn:         http://tun.in/pjSaU JioSaavn:     https://www.jiosaavn.com/shows/lets-get-lost/1/IeeKc0SILHM_

Bem-vindo! Let's bring back the funk to LGL and experience entirly "One Album At A Time". Release details under: https://www.discogs.com/master/232166-Zalmac-Whatcha-Gonna-Do No script. Nothing planned or rehearsed. All music genres. Real time impressions under the influence in meditation posture. Stay Love. Patreon:       https://www.patreon.com/Lets_Get_Lost Amazon: https://music.amazon.de/podcasts/57cd4c31-1730-4535-99b8-6b1d219a49c4/LETS-GET-LOST Audible: https://www.audible.com/pd/Podcast/ B08K573BJ9 TuneIn:         http://tun.in/pjSaU JioSaavn:    https://www.jiosaavn.com/shows/lets-get-lost/1/IeeKc0SILHM_ --- Send in a voice message: https://podcasters.spotify.com/pod/show/lets-get-lost/message

Premiera, 14. marec 2024 Radio Slovenija, informativne oddaje V Lutkovnem gledališču Ljubljana so sinoči uprizorili predstavo Mišek Julijan, ki jo je po motivih slikanice Joeja Todd-Stantona, režirala Asja Kahrimanović. O režijskem prvencu lutkovne umetnice, animatorke in igralke, dolgoletne članice te lutkovne ustanove, več Magda Tušar: Dramaturg: Benjamin ZajcLikovna podoba: Jure EngelsbergerScenografka: Katarina PlanincGlasba: Zajtrk (Sven Horvat, Laura Krajnc)Oblikovalka luči: Maša AvsecLutkovni tehnolog: Žiga LebarAsistentka scenografije: Laura KrajncNastopajo: Gašper Malnar, Zala Ana Štiglic, Ajda TomanVodja predstave in oblikovalec zvoka:Aleš ErjavecProducentka:Alja Cerar MihajlovićLučno vodstvo: Maša AvsecScenski tehnik: Domen NagodeVodja delavnic LGL: Zoran SrdićIzdelava lutk, scene in kostumov: Žiga Lebar, David Klemenčič, Katarina Planinc, Laura Krajnc, Sandra Birjukov, Marjeta Valjavec, Zala Kalan, Uroš Mehle 

Will Detroit sink the Buccaneers ship? The Bucs come in as winners of 6 of their last 7 games. The Lions look to feed off the energy of the long awaited playoff win to get another W at home. We discuss if the Eagles or Cowboys had a bigger collapse and congratulate Mike McCarthy on earning another season as head coach. We rank our Rookies of the Year and give our picks for the NFL Divisional Round of the Playoffs. Happy Episode 100 to all who celebrate. Thank you Shareholders, LGL. --- Support this podcast: https://podcasters.spotify.com/pod/show/thelionssharepodcast/support

Mateja Bučar je slovenska koreografija in plesalka, ki je svojo umetniško pot začela kot balerina v Operi in baletu SNG v Ljubljani, pozneje in vzporedno je bila članica Plesnega teatra Ljubljana. Baletno izobrazbo je pridobivala doma in v tujini, prav tako tehnike modernega in sodobnega plesa. Z letom 1992 stopi na samostojno umetniško pot koreografinje in plesalke. Od leta 1999 pod okriljem DUM, Društva umetnikov, so njena dela s področij sodobnega plesa in performansa uprizarjana v mednarodnem prostoru. Mateja Bučar je leta 2015 na Trinity Laban konservatoriju za glasbo in ples londonske univerze prejela doktorski naziv na področju teorije estetskih vprašanj v koreografskih praksah. Svoje umetniško delo ustvarja za različne notranje in zunanje ambiente. Več pa v pogovoru, ki ga bomo poslušali v dveh delih. Vabimo vas k poslušanju! fotografija iz predstave Rdeči čeveljci / Rdeči oktober, LGL, 2021, foto: Jaka Varmuž, www.dum-club.si

Herzlich Willkommen. Let's depart on this journey through my nine-episode "ASMR Chills" series. This OAAAT (embodying all LGL aspirations) is a first impression and serves as prelude to this series exclusively released on Anchor. Original title was going to be OAAAT - Healing Reassurance. I acknowledge Brian Jackson and highly recommend his album "This Is Brian Jackson" (2022). No script. Nothing planned or rehearsed. All music genres. Real time impressions under the influence in meditation posture. Stay Love. Patreon:            https://www.patreon.com/Lets_Get_Lost Amazon:           https://music.amazon.de/podcasts/57cd4c31-1730-4535-99b8-6b1d219a49c4/LETS-GET-LOST Audible:            https://www.audible.com/pd/Podcast/B08K573BJ9 --- Send in a voice message: https://podcasters.spotify.com/pod/show/lets-get-lost/message

Herzlich Willkommen. A perfect blend of everything from Let's Get Lost on my nine-episode "ASMR Chills" series. This OAAAT from the Far East is a first impression and a long time favourite of mine you might have heard before on LGL. My original title was going to be OAAAT - Wow, Vow Wow!. No script. Nothing planned or rehearsed. All music genres. Real time impressions under the influence in meditation posture. Stay Love. Patreon:            https://www.patreon.com/Lets_Get_Lost Amazon:           https://music.amazon.de/podcasts/57cd4c31-1730-4535-99b8-6b1d219a49c4/LETS-GET-LOST Audible:            https://www.audible.com/pd/Podcast/B08K573BJ9

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2023.07.10.548302v1?rss=1 Authors: Richardson, H. E., Portela, M., Mukherjee, S., Paul, S., La Marca, J. E., Parsons, L. M., Veraksa, A. Abstract: The tumour suppressor, Lethal (2) giant larvae (Lgl), is an evolutionarily conserved protein that was discovered in the vinegar fly, Drosophila, where its depletion results in tissue overgrowth and loss of cell polarity and tissue architecture. Our previous studies have revealed a new role for Lgl in linking cell polarity and tissue growth through regulation of the Notch (proliferation and differentiation) and the Hippo (negative tissue growth control) signalling pathways. Moreover, Lgl regulates vesicle acidification, via the Vacuolar ATPase (V-ATPase), and we showed that Lgl inhibits V-ATPase activity through Vap33 (a Vamp (v-SNARE)-associated protein, involved in endo-lysosomal trafficking) to regulate the Notch pathway. However, how Lgl acts to regulate the Hippo pathway was unclear. In this current study, we show that V-ATPase activity inhibits the Hippo pathway, whereas Vap33 acts to activate Hippo signalling. Using an in vivo affinity-purification approach we found that Vap33 binds to the actin cytoskeletal regulators RtGEF (Pix, a Rho-type guanine nucleotide exchange factor) and Git (G protein-coupled receptor kinase interacting ArfGAP), which also bind to the Hpo protein kinase, and are involved in the activation of the Hippo pathway. Vap33 genetically interacts with RtGEF and Git in Hippo pathway regulation. Additionally, we show that the ADP ribosylation factor Arf79F (Arf1), which is a Hpo interactor, is involved in the inhibition of the Hippo pathway. Altogether our data suggests that Lgl acts via Vap33 to activate the Hippo pathway by a dual mechanism, 1) through interaction with RtGEF/Git/Arf79F, and 2) through interaction and inhibition of the V-ATPase, thereby controlling epithelial tissue growth. Copy rights belong to original authors. Visit the link for more info Podcast created by Paper Player, LLC

The ASX 200bounced back after sliding to a two-month low during trade closing up 20 points to 7111 (+0.3%), as sentiment improved after the House of Representatives passed the bill to raise the debt ceiling with bipartisan support. Resources and base metals clawing back gains from yesterday's losses, BHP +0.1%, RIO +0.4% and FMG up 0.8%. Lithium mixed, but mostly down MIN down 3.6% and PLS down 1.6%. Gold miners are today's winners as the likelihood of another Fed rate hike increases. NST 3.1%, NCM 2.4%, EVN 0.6%. Financials showing modest gains, with the Big Bank Basket down to $166.17 (-0.1%), CBA, flat WBC off 0.4% and NAB up 0.3%. Tech stocks didn't stay down for long, with the All-Tech Index up 0.7%, SQ2 +2.3% XRO +1.9% and WTC +0.9%. Industrials were mixed and uninspiring, ALL rose 1.3%, SEK up 0.6% and REA off 2.0%. Defensives did ok with WOW and COL up. Energy sector up 0.4%, despite oil dropping for a third consecutive session. In corporate news, NMT jumped 21.9% after providing an update on its joint venture for lithium-ion battery recycling. LGL rose 16.1% after raising full-year guidance. AGY dropped 6.3% despite announcing that the Rincon Lithium project is now partially operational. In economic news, Australian retail sales were unchanged at $35.3bn in April, indicating that retail has hit a plateau. Caixin China's general manufacturing PMI unexpectedly rose to 50.9 in May from 49.5 in the previous month, while exceeding consensus, the latest results highlights the patchy economic recovery amid insufficient demand and deflation risks. Asian markets are up, Japan's Nikkei rises on bargain hunters up 0.9% and HK up 0.6% with China up 0.3%. Australia 10Y yield up 3.62%. Dow Jones futures down 21 points and Nasdaq futures down 11 points. Why not sign up for a free trial? Get access to expert insights and research and become a better investor. Make life simple. Invest with Marcus Today.

Larry takes your calls and comments on your favorite regular LGL guests in hour 3.  See omnystudio.com/listener for privacy information.

Francesco De Stradis from Ord Minnett and Mark Gardner from Maqro Capital go in-depth and stock specific. Stocks covered: TPG, DDR, EDV, LGL, BGA, TRS, CTD, WEB, BAP, EVT. The stock of the day is Marley Spoon (MMM). Hosted on Acast. See acast.com/privacy for more information.

Stefan Bernstein CEO of GreenRoc Mining #GROC discusses successful phase 2 drilling at Amitsoq Project which more than doubles graphite deposit footprint. Highlights · Drilling was completed on 17 September 2022, with 19 holes drilled for a total of 2,844m. · All holes reached target depths and intersected significant graphite layers, extending the deposit to the west by about 100m and to the northwest and the north by about 150m in horizontal directions, more than doubling the deposit footprint, which continues to remain open. o Lower Graphite Layer ("LGL") shows mineable thicknesses greater than 2.0m in all 19 holes with true thickness (where present) varying between 2.50m and 20.69m. o Upper Graphite Layer ("UGL") shows mineable thicknesses greater than 2.0m in eight holes with true thickness varying between 2.72m and 7.99m. · The LGL appears to form the main orebody of the deposit with surface outcrops suggesting a potential continuation of significant LGL thicknesses for several hundred meters towards the north.  · Exceptionally high grades of up to 23.01% graphitic carbon (C(g)) in the LGL and up to 19.83% C(g) in the UGL were returned from Phase 1 and visual inspection of drill core suggest that Phase 2 intersections are similar in ore grade. · Results are expected to support a significant upgrade in the Project's resource tonnage, with analytical results expected Q4 2022. · Despite extensive drilling, the deposit remains open towards the north, west and southwest, giving an indication of the significant resource potential. To read the full RNS click here

Primož Ekart, igralec in režiser, je nedavno v razpadajoči Palači Cukrarna v Ljubljani režiral in za gledališki prostor priredil intermedijsko uprizoritev z lutkami Severni sij.Erdman svojevrstno dramsko, gibalno, vizualno – zvočno interpretacijo romana Draga Jančarja. V predstavi se historični podatki prepletajo z domišljijskimi elementi, uporabljene so tudi maske.Gre za večpomenskost uporabljenih motivov, ki upodabljajo kaotičnost sveta, nekdaj in danes ter za iskanje zatočišč pred našimi notranjimi in zunanjimi beganji. Intermedijsko zasnovan koncept predstave ustvarja dinamična razmerja in sozvočja med številnih uprizoritvenimi izrazi, vizijami, jeziki, pripovednimi paradigmami in perspektivami. V uprizoritveni manifestaciji časovno in krajevno določene družbene anomije – leta 1938 v Mariboru, se namreč nizajo tako video projekcije, dialoški in monološki dramski elementi, kot koreografija groteskne maškarade in morbidnega dramoleta denimo… Raztrgana duševnost psihotičnega prišleka, ki se v tem mestu metaforično želi povezati s popkovino svojega otroštva, se v nenehnih poskusih bežanja pred morastimi prikaznimi in srhljivo preroškimi sanjami, pokrije z zastrupljenim družbenim ozračjem. Gre namreč za dekadentno obdobje slutenj skorajšnjega razdejanja, kolektivne zmede in etične mizerije, stanje nekontrolirane divje skupnostne patologije, ki se v seštevku individualnih nezavednosti materializira v zločinu razčlovečenih steklih zveri in v poznejši realizaciji masakra globalnih razsežnosti. V predstavi občutenega kafkovskega vzdušja, katere oder je označen z marginalnostjo, kot bi bil ozvočen z glasovi nekdanjih stanovalcev družbenega roba, se prepleta množica opredmetenih pomenov in mističnih, ezoteričnih in metafizičnih simbolov; med njimi izstopata izmikajoča se modra krogla in historično dokumentiran pojav severnega sija. Poosebljeni gibalci dogajanja (kaotičnost je najbolj potencirana v nervoznem gostilniškem prostaštvu) pa so pričevalci in kronisti, t.i. oči mesta, ki s časovnimi preskoki beležijo spremembe in prelome. Tako angažiranemu in nagovorjenemu gledalcu, igralci in animatorji skoraj ne dovolijo pasivnosti v spremljanju predstave, ki ne glede na bogato asociativnost, ne sili v dekodiranje sodobnega aktualnega stanja v svetu. V oddaji Kulturni fokus, del oddaje je bil posnet tudi za oddajo Oder, bo Primož Ekart več povedal tudi o drugih izkušnjah z odrom, literarnimi adptacijami, mladimi ustvarjalci in nasploh z raziskovanji sodobnega gledališča in animiranih form. foto: LGL (iz predstave Severni sij)

V razpadajoči Palači Cukrarna v Ljubljani je bila nedavno premiera intermedijske uprizoritve z lutkami Severni sij.Erdman,svojevrstna dramska, gibalna, vizualno – zvočna interpretacija romana pisatelja Draga Jančarja. V predstavi se historični podatki iz dela, ki sodi v slovenski kanon, prepletajo z domišljijskimi elementi, uporabljene so tudi maske... O večpomenskosti uporabljenih motivov, ki upodabljajo kaotičnost sveta nekdaj in danes ter o iskanju zatočišč pred našimi notranjimi in zunanjimi beganji, v oddaji Oder. Gosta bosta Darja Hlavka Godina, ki je predstavi dala zvočno in glasbeno podobo in režiser Primož Ekart, ki je besedilo tudi priredil za odrski prostor. foto: LGL

Stefan Bernstein CEO of GreenRoc Mining #GROC discusses how the ongoing drilling programme at their Amitsoq Project, one of the highest-grade graphite deposits in the world, substantially extends the deposit. Highlights · As of 14 August 2022, a total of nine holes have been completed for a total of 1,360m. · All holes reached target depths and intersected the targeted graphite layers, thereby extending the deposit to the west by about 100m and to the northwest by about 150m, essentially doubling the deposit footprint. · The deposit remains open towards the northwest, west and southwest. · Several intersections are at least as good as those obtained in the 2021 Phase 1 drill programme, thus confirming the significant thickness of both the Upper Graphite Layer ("UGL") and Lower Graphite Layer ("LGL"). · To date, 16 intersections of UGL and LGL greater than 3.0m each have been drilled. · All holes have at least one mineable graphite layer intersection greater than 5.25m. · Visual inspection of the drill core suggests that the intersections are similar in ore grade to the Phase 1 results, which returned grades of up to 23.01% C(g) in the LGL and up to 19.83% C(g) in the UGL. · Drilling remains ongoing, with the programme expected to conclude by the end of September. · Drilling to date gives strong confidence that a higher-tonnage, higher-category Resource can be established ahead of a feasibility study. To read the full RNS click here

G'Day! I am finally sharing my first attempt at classical music for LGL with "THE" legendary german composer. This is certainly a new level of complexity i need some time to get used to. For me it is less about the music and more about the live feeling of the recording! Release details under: https://www.discogs.com/release/6314022-Naum-Starkman-Entangled-Devotions No script. Nothing planned or rehearsed. All music genres. Real time impressions under the influence in meditation posture. Stay Patreon: https://www.patreon.com/Lets_Get_Lost Amazon: https://music.amazon.de/podcasts/57cd4c31-1730-4535-99b8-6b1d219a49c4/LETS-GET-LOST Audible: https://www.audible.com/pd/Podcast/B08K573BJ9 Podcast Addict: https://podcastaddict.com/podcast/3057136 TuneIn: http://tun.in/pjSaU Podchaser: https://www.podchaser.com/LETSGETLOST JioSaavn: https://www.jiosaavn.com/shows/lets-get-lost/1/IeeKc0SILHM_

Episode 1 features Dorian Banks, the CEO of Looking Glass Labs, the parent company that owns the NFT project House of Kibaa. Dorian discusses his journey into crypto and NFTs including how he became the CEO of LGL. He talks about building out metaverses using Unreal Engine, running point on coordinating a live-action production, trying to get back into reading as an escape, loving to cook and host dinner parties, and how his Bored Ape was sold while he was on a camping trip with friends!

Estijos aktualijas aptariame su LRT RADIJO bendradarbiu šioje šalyje Vaidu Matulaičiu.Kas nusipelnė svarbiausių žmogaus teisių apdovanojimų Lietuvoje? Portale lrt.lt iki balandžio 24 d. galite balsuoti Nacionaliniuose lygybės ir įvairovės apdovanojimuose 2020–2021. Pokalbyje dalyvauja Lygių galimybių kontrolieriaus tarnybos Diskriminacijos prevencijos ir komunikacijos grupės vadovė Mintautė Jurkutė, LGL direktorius Vladimir Simonko,Moterų informacijos centro direktorė Jūratė Šeduikienė.80 procentų pabėgėlių iš Ukrainos patiria psichologinių sunkumų, jie neretai kenčia nuo potrauminio streso. Vilniaus miesto psichikos sveikatos centre kuriamas Psichotraumatologijos centras, padėsiantis ir ukrainiečiams. Apie tai su Vilniaus miesto psichikos sveikatos centro direktoriumi Martynu Marcinkevičiumi.Ukrainoje sulaikytas prokremliškas oligarchas Viktoras Medvedčukas ragina jį iškeisti į Ukrainos pajėgų narius, kovojančius Mariupolyje.Lenkijos – Ukrainos pasienis. Dorohusko-Jagodino kontrolės punktas. Kulkų žymių nusėtame automobilyje – trys mažamečiai ir jų tėvai – Irina su Aleksandru. Dramatiška kelionė iš apsiausto, griuvėsiais ir kapais virtusio Mariupolio truko trylika dienų. Ukrainiečių šeimą, kieme laidojusią kaimynus, besimaitinusią balandžiais ir gėrusią vandenį iš radiatorių, Lenkijos-Ukrainos pasienyje kalbino Ana Grigoit ir Daumantas Butkus.Ved. Agnė Skamarakaitė

Bayern ist das erste und bislang einzige Bundesland mit elektronischem Pollenmonitoring. Welche Daten hier genau gemessen werden und warum sich der Pollenflug durch den Klimawandel verändert, das erklärt uns in dieser Folge Prof. Jeroen Buters vom Zentrum für Allergie und Umwelt der TUM. Außerdem sprechen wir mit Dr. Susanne Kutzora. Sie ist wissenschaftliche Mitarbeiterin beim Landesamt für Gesundheit und Lebensmittelsicherheit (LGL). Das LGL setzt ePIN, das elektronische Polleninformationsnetzwerk zur Erfassung des Pollenflugs in Bayern, federführend um.

In our final stop in our Cytopenias series, we discuss the ins and outs of neutropenia. This is another very commonly seen issue in the clinic and in the hospital so most definitely high yield!Why is neutropenia dangerous?Prone to infections, especially gut translocation of bacteriaDefinition of neutropenia:NORMAL: WBC 4400-11000 cells/microL; neutrophils make up 40-70% of thatNeutropenia defined by ANC: WBC (cells/microL) x percent (PMNs + bands) ÷ 100 Breakdown:Neutropenia: ANC

Mark Moreland from Teaminvest and Andrew Wielandt from DP Wealth Advisory go in-depth and stock specific. Stocks covered: AZJ, EOS, PNI, MYR, A2M, 360, CTD, MTS, FPH, LGL. Our stock of the day is Uniti Group (UWL).Subscriber survey: https://ausbiz.co/survey See acast.com/privacy for privacy and opt-out information.

Chris Conway from Marcus Today and Mark Gardner from Maqro Capital go in-depth and stock specific. Stocks covered: LVH, GNC, PPG, TLS, WAM, LGL, TPW, CSL, WR1, SRV. Stock of the day is Latitude Group Holdings (LFS). See acast.com/privacy for privacy and opt-out information.

Kirk Adams CEO of GreenRoc Mining #GROC talks through results from its Amitsoq Graphite Project that show the graphite layers to be both thicker, and more consistent, than previously thought. Key Points · High-grade graphite mineralisation confirmed and increasing true widths observed down dip on both the upper and lower graphite layers, supporting the potential for a sizeable JORC Resource · Lower Graphite Layer (LGL) returns drill intercepts grading up to 23.01% C(g) and channel samples grading up to 30.35% C(g) ·  Upper Graphite Layer (UGL) returns drill intercepts grading up to 19.83% C(g) and channel samples grading up to 27.40% C(g) · Significant true width thickness of graphite layers confirmed by drilling, including: o  LGL: 15.60 m @ 22.34% C(g) from 93.61 m  o  LGL: 14.95 m @ 22.82% C(g) from 102.19 m · Deposit open along strike (predominantly to the north) and down dip, which will be tested in Phase 2 drilling programme  · Significant down-dip extensions and increase in thickness from surface outcrop · Strike length of LGL intersected from Pads B and E totals 154 m · Low-grade uranium content poses no problems for future development under new Greenlandic legislation · Drilling campaign confirms Amitsoq to be one of the highest-grade graphite deposits globally · These results will be a key element in the mineral resource estimation assessment to be made by the Competent Person in due course. GreenRoc's Mining Assets GreenRoc Mining Plc is an AIM-quoted company which is developing mining projects in Greenland in critical, high-demand and high-value minerals. Led by a group of highly experienced mining industry professionals, GreenRoc has a portfolio of 100% owned projects: Amitsoq Graphite, Thule Black Sands Ilmenite, Melville Bay Iron and Inglefield Multi-Element.

Pasipiktinimo ir prašymų pripažinti nepageidaujamu šalyje sulaukęs Luganskis Lietuvoje nekoncertuos dėl traumos.LGL konferencija „Šeimų apsauga ir įvairovė demokratinėje visuomenėje: problemos ir perspektyvos“.167 afganistaniečiams suteiktas prieglobstis Lietuvoje.Ved. Olga Ugriumova

Jun Bei Liu from Tribeca Investment Partners and Adam Dawes from Shaw and Partners go in-depth and stock specific. Stocks covered: WOW, BOE, LGL, APA, MGF, EVN, ADO, RFG, BAP, EGG. Our stock of the day is Aventus (AVN). See acast.com/privacy for privacy and opt-out information.

As-salamu alaykum, if i could never again do an Essential Mix for LGL i would still be glad for having done this one. Pure first impression (i was clueless about Maya) and i was unable to resist her beautiful sounds. This is only the first hour of her Essential Mix debut from July 2011. I cherish her music and will definitely return for more. I like to express my acknowledgement to Radio1's Essential Mix hosted by Pete Tong, the british artist Maya Jane Cole and the crew of TALiON! Real time impressions under the influence in meditation posture. Stay

Bienvenue dans ce nouvel épisode de Fait Main, le podcast qui part à la rencontre des entrepreneurs créatifs !Je m'appelle Mélanie, j'adore les arts créatifs et je suis heureuse de partager avec vous mes conversations avec des créatrices et des créateurs que vous suivez peut-être comme moi sur Instagram. Qu'ils créent avec du papier, du tissu ou de la laine, avec des crayons, des aiguilles, des pinceaux ou d'autres outils, découvrir leur parcours et les coulisses de leur entreprise créative est un régal d'inspiration

Welcome to some more funky LGL of 2021 where i listen to all kinds of music and completely lose my mind for "Let's Get Lost". I especially like the funky twist at the end ;D No script. Nothing planned or rehearsed. All music genres. Real time impressions under the influence in meditation posture. Stay Love. Patreon: https://www.patreon.com/Lets_Get_Lost Podcast Addict: https://podcastaddict.com/podcast/3057136 TuneIn: http://tun.in/pjSaU Podchaser: https://www.podchaser.com/LETSGETLOST JioSaavn: https://www.jiosaavn.com/shows/lets-get-lost/1/IeeKc0SILHM_

Welcome to the first LGL of 2021 where i listen to 70's fusion and completely lose my mind for "Let's Get Lost". I recorded this on my PC during some of my newest Let's Watch episodes. I hope to record on my main system soon! No script. Nothing planned or rehearsed. All music genres. Real time impressions under the influence in meditation posture. Stay Love. Patreon: https://www.patreon.com/Lets_Get_Lost Podcast Addict: https://podcastaddict.com/podcast/3057136 TuneIn: http://tun.in/pjSaU Podchaser: https://www.podchaser.com/LETSGETLOST JioSaavn: https://www.jiosaavn.com/shows/lets-get-lost/1/IeeKc0SILHM_

»Pravljice ne živijo le v neštetih različicah različnih pripovedovalcev, ampak celo v mnogih različicah istega pripovedovalca.« Spremeni se poudarek, spremeni se pomen. Pretočna in živa se ne pusti ujeti v »v dokončne in enopomenske sodbe«. Pravljica, ki je kaj vredna. O pripovedovanju nekoč in danes, o občutku za zgodbo, njeno obliko in njeno sporočilnost pa več v oddaji Oder. Naša gostja bo pesnica, pisateljica in pripovedovalka Anja Štefan. Foto: Urška Boljkovac, LGL (iz lutkovne predstave Štiri črne mravljice po pravljici Anje Štefan)

The Paleo Cardiologist, Dr. Jack Wolfson, joins the show today to debunk myths about saturated fat and its impact on heart health. He talks about which fats are important to include in your diet for optimal levels of HGL and LGL and how he discovered a paleo lifestyle through recognizing flaws in modern medicine. Plus, he and Dr. Axe recommend their top supplements.

In this week's episode, Stephie's husband, Steven, shares a powerful testimony of God moving in his life. He was diagnosed with a chronic disease a few years ago that brought fear of what the future would hold. Listen in to hear how God knew the full story and had a plan all along. As always, keep the faith, and see the joy. Special thanks to our music creators: @mbb_music and @7obu Instagram: @joyunseenpodcast @jaimemcguire_ @stephieclement Facebook: https://www.facebook.com/joyunseenpodcast

»Če pomislimo, da ima otrok pravico do hrane in varnega zavetja, do izobrazbe in igre, potem lahko dodamo tudi, da ima otrok na poti do svoje uresničitve pravico tudi do kulture in umetnosti. Tako spoznava življenje ne le racionalno, pač pa skozi različne zaznave in čustva na načine, ki mu ponudijo življenje bolj polno in lepo, kot če so za umetnost prikrajšani,« v članku z naslovom Otroci za zdrav in optimalen razvoj potrebujejo umetnost zapiše dr. Tina Bregant. Različnim vidikom razvejanega in kompleksnega področja gledališke produkcije za otroke in mlade bomo pozornost namenili tudi v ciklu oddaj Oder, ki bodo na sporedu enkrat mesečno. V uvodni oddaji bomo osvetlili stanje v širši krajini performativnih praks, namenjenih najmlajšemu občinstvu, predstavili bomo nekaj primerov dobrih praks kulturnovzgojnih projektov in se dotaknili tako umetniških kot tudi produkcijskih izzivov tega pomembnega področja ustvarjanja za najmlajše. Gostji: mag. Mojco Jan Zoran, direktorica Slovenskega gledališkega inštituta in medijska psihologinja, mag. Martina Peštaj, urednica Otroškega in mladinskega programa na Televiziji Slovenija. Nekje drugje, LGL, foto Jaka Varmuž Nagrada za najboljšo otroško predstavo na 18. festivalu uprizoritvenih umetnosti za otroke in mlade Zlata paličica, Ljubljana, 2019

Hosting a nonprofit event sounds easy...until you actually do it! There are a million little pieces that have to come together for the big day, and I wanted to talk about all of them. There was so much great information in my interviews this week that I have to break this episode into two parts. In Part One, we’ll talk about how you can improve your next fundraising event with donor data and how to welcome newcomers who are attending their first event with you. Our first guest is Timi Paccioretti, the Senior Marketing Manager of Little Green Light. LGL is my favorite donor management platform, and Timi gives advice on how to use your donor management system to improve your events. She shares tips about segmenting your target audience, capturing event attendee information, tracking event outcomes, and more. Next, we chat with Holly Clark, a seasoned nonprofit events planner and owner of Holly Clark Events. Holly walked me through the details of how to plan and coordinate three very different types of nonprofit events: a “lunch and learn,” an upscale signature event, and an outdoor holiday event. In Part One, you’ll learn why Lunch & Learns are timeless and effective, even if you may think they sound a little outdated. Holly also offers some great advice on incorporating strong a strong call-to-action at your Lunch and Learn by using the examples of Safe Haven Family Shelter and Renewal House. Be sure to subscribe to the Nonprofit Jenni Show so you won’t miss Holly’s advice on hosting signature and outdoor events! Our sponsors this episode are:--Little Green Light, Jenni’s favorite donor management system. Check out their free resource library full of ebooks about fundraising at www.littlegreenlight.com/jenni and use the discount code JENNI to receive $150 off your first year of donor management services.--DipJar, Jenni’s favorite alternative to cash donation boxes. Empower your donors to make credit card donations with DipJar’s fun light-up donation gadget! Visit dipjar.com/jenni for a 5% discount on your first purchase.  Nonprofit Jenni provides consulting services and coaching calls for nonprofits and social impact organizations seeking guidance in the areas of Marketing, Fundraising, Growth, and Starting Up. Visit nonprofitjenni.com to learn more. All information in the Nonprofit Jenni Show is very general in nature, and may not apply to your specific nonprofit organization. Please direct clarifying questions to Nonprofit Jenni by visiting nonprofitjenni.com. Produced by Wayfare Recording Co. Music by Emily Summers. ©2020 Nonprofit Jenni. All Rights Reserved.

V vsaki od enajstih epizod serije Abonma Bienale v realnem času predstave opisujemo in komentiramo po eno od tekmovalnih predstav 10. Bienala lutkovnih ustvarjalcev Slovenije. Martina Krpana spremljajo: Klara Drnovšek Solina, študentka primerjalne književnosti in južnoslavistike, Zala Dobovšek, teatrologinja, Maša Radi Buh, študentka sociologije kulture in Nika Leskovšek, selektorica. Montaža in oblikovanje zvoka: Aleš Zorec Tehnična podpora: Boštjan Eržen, Youreup radio Sočasno predvajanje: Radio MARŠ Medijska podpora: Radio Maribor Lutkovno gledališče Ljubljana Martin Krpan Avtor: Fran Levstik Režiser: Tin Grabnar Avtorica likovne podobe: Špela Trobec Avtorji priredbe: Ana Duša, Tin Grabnar, Brina Klampfer Dramaturginja: Brina Klampfer Igrajo: Maja Kunšič, Martina Maurič Lazar, Zala Ana Štiglic, Marjan Stanić k. g. (tolkala) Avtorica songov in korepetitorica: Ana Duša Scenografa: Tin Grabnar, Špela Trobec Svetlobno oblikovanje: Igor Remeta Avtor glasbe: Marjan Stanić Songe uglasbil: Mitja Vrhovnik Smrekar Kostumografka: Sara Smrajc Žnidarčič Lektorica: Metka Damjan Vodja predstave in oblikovalec zvoka: Erik Krkač Producentka: Ana Rokvić Pinterič Lučno vodstvo: Maša Avsec Scenski tehnik: Jure Popovič Izdelava lutk, kostumov in scenografije: Zoran Srdić, Iztok Bobić, Zala Kalan, Polona Černe, Sandra Birjukov, Marjeta Valjavec, Igor Remeta, Olga Milič, Špela Ulaga, Hana Juta Kozar, Žiga Lebar, Špela Trobec, Jože Lašič, Ivan Božičko (CNC), Cveto Kuniševič (3D sken), Nejc Šubic, Vladson, Zlatko Djogić, Lara Mastnak, Uroš Mehle s. p. Martin Krpan spremlja delovanje Lutkovnega gledališča Ljubljana že vse od njegovih začetkov, saj je to predstava, s katero so ljubljanski lutkarji odprli svoje prve prostore Mestnega lutkovnega gledališča (danes LGL) leta 1950 na Levstikovem trgu, zato jo včasih imenujemo prva ”prava” predstava Lutkovnega gledališča Ljubljana, čeprav je gledališče delovalo že od leta 1948. V novi zasedbi in novi likovni podobi je bil Martin Krpan tudi prva predstava leta 1984, ko so si ljubljanski lutkarji po dolgih letih le izborili prostor v Mestnem domu, kjer gledališče domuje še danes. Prva predstava novonastalega gledališča je bila sicer Udarna brigada, ki so jo kot otvoritveno predstavo Ljubljanskega festivala uprizorili oktobra 1948. Tokrat se je to temeljno delo slovenske literature na oder vrnilo v sodobni interpretaciji mladega režiserja Tina Grabnarja, ki ga obiskovalci Lutkovnega gledališča Ljubljana že poznajo po nagrajeni predstavi Nekje drugje. Martin Krpan, ki s svojo kobilo skrivoma tihotapi sol, bleščeče cesarsko življenje na dunajskem dvoru, nonšalantno sekanje prečudovite dvorne lipe in divji dvoboji ponujajo čudovito izhodišče za lutkovno ustvarjanje. Uporabljena je tradicionalna tehnika ročnih lutk. Zgodba se odvija na klasičnem prizorišču za ročne lutke imenovanem kastelet.

In this episode LGL spits some bars as he is on his nightly walk with Sekoya. He drops what motivated him early for life, what it’s like when teammates don’t practice, and never forgetting WHAT you came from! Tell a friend to tell a friend to get those ears dialed!!

In this episode LGL is HYPE. He lets loose for the longest pod yet as he discusses off the leash training vs on the leash. Gives a nod to his peers and friends living life off the leash and being very successful. He also stops Sekoya from destroying a ground hog!!

LaxGodLinder GOES OFF. Listen closely as LGL begins to get fired up about ... walking his dog?

In this episode Sekoya is feeling frisky and Almost gets clapped by a car... more importantly LGL discusses the ride Raj and him talked about. Also, an Uber driver gave some life SHIFTING advice !! Tune in !!

In the FIRST installment of Walk Your Dawg podcast, you’ll hear barking dogs, birds chirping, and LGL talking briefly about dealing with recent demons on the way to the top and how HE chose to overcome them. LGL also gives insight to his new streak of constant happiness and positive energy!

Can you spot the difference between WPW and LGL on an EKG? How are WAP and MAT different? Where'd the p-wave go in junctional rhythms? How do you treat the various presentations of Afib (stable vs unstable pt)? Is SVT *always* narrow complex QRS? How do you keep straight the two different types of 2nd degree heart block? PA Kay has an EKG test in 48 hours, and she hasn't studied EKGs in 5 months. Come along on her panic-studying journey and learn the answers to these questions, plus a whole bunch more. In this episode, PA Kay takes you through a hefty review of a few basic EKG topics before escalating quickly into arrythmias, MIs, and their treatment. 

¡Hola Branders!. ¿Sabéis qué tienen que ver Julio César, Gutenberg, Baskerville, el crowdfunding y el departamento de Defensa de Estados Unidos, con la arroba y el correo electrónico?... esta semana os cuento la historia de la marca Acumbamail y el origen del Newsletter. Recordad que podéis apoyar nuestro trabajo realizando vuestras compras en Amazon a través del enlace de afiliados que tenemos en nuestra página web; y haciéndoos mecenas de BrandStocker. Ya sabéis que nos podéis seguir en Twitter, en Facebook e Instagram, donde nos encontraréis con el usuario BrandStocker. Y por supuesto no olvidéis comentar este programa, darle a “me gusta” y compartirlo en vuestras redes sociales. https://brandstocker.com/podcast/acumbamail-y-el-origen-del-newsletter/

¡Hola Branders!. ¿Sabéis qué tienen que ver Julio César, Gutenberg, Baskerville, el crowdfunding y el departamento de Defensa de Estados Unidos, con la arroba y el correo electrónico?... esta semana os cuento la historia de la marca Acumbamail y el origen del Newsletter. Recordad que podéis apoyar nuestro trabajo realizando vuestras compras en Amazon a través del enlace de afiliados que tenemos en nuestra página web; y haciéndoos mecenas de BrandStocker. Ya sabéis que nos podéis seguir en Twitter, en Facebook e Instagram, donde nos encontraréis con el usuario BrandStocker. Y por supuesto no olvidéis comentar este programa, darle a “me gusta” y compartirlo en vuestras redes sociales. https://brandstocker.com/podcast/acumbamail-y-el-origen-del-newsletter/

Dr Roe speaks with ecancertv at ASH 2016 about genotyping and treatment outcomes for patients with myelodysplastic syndrome with T cell large granular lymphocyte proliferations (LGL). She describes characterisation of patients by age and risk, with a greater response to lenalidomide from patients without LGL clonal types.

Local Government Liaison (LGL) Cadre Lead Patsy Fletcher and LGL Subject Matter Experts CJ Hamilton and Todd Miller discuss the skills and capabilities of this cadre, what their mission is, and the wide variety of deployment experiences that LGLs may face.

Polarity is a fundamental feature of almost all cells. It generally refers to the asymmetric organization of several cellular components. The plasma membrane, for example, exhibits both a transbilayer and a lateral asymmetry in most eukaryotic cells. Lipids are asymmetrically distributed between the cytoplasmic and the extracellular leaflet of the membrane and segregate laterally together with specific proteins to form dynamic nanoscale assemblies, known as rafts. Polarity can also specifically describe the asymmetric distribution of key molecules within a cell. These molecules, known as polarity determinants, can orient a multitude of specialized cellular functions, such as cell shape, cell division and fate determination. In the framework of this thesis, we aimed to reconstitute essential features of membrane unmixing and cell polarity with a "bottom-up" synthetic biology approach. We worked with both: pure lipid systems, whose unmixing is driven by the asymmetric distribution of lipids in the two leaflets, and a lipid-protein system, whose polarization is instead due to reaction-diffusion mechanisms. In both cases, we used Giant Unilamellar Vesicles (GUVs) and Sup- ported Lipid Bilayers (SLBs) to model biological membranes and employed modern biophys- ical techniques, such as fluorescence correlation spectroscopy, to quantitatively characterize lipid bilayers and protein-lipid interactions. In the pure lipid systems, we first reconstituted membrane transbilayer asymmetry, applying a cyclodextrin-mediated lipid exchange method, which enables us to enrich membranes with lipids of choice. The enrichment of the membrane with sphingomyelin and/or cholesterol triggers the segregation of lipids into two coexisting asymmetric phases both in SLBs and GUVs, whereas exchanging different amounts of phosphatidylglycerol with the outer leaflet of the GUV membranes controls vesicle shape. Tuning the lipid content of model membranes revealed that small changes in the composition of one leaflet affect the overall lipid miscibility of the bilayer and that membrane shape transformations are possible also in absence of a protein machinery and as a consequence of the lipid redistribution in the membrane. In the protein-lipid system, we aimed to reconstitute a minimal polarization system inspired by the C. elegans embryo at one-cell stage, which polarize along the anterior-posterior axis by sorting the PARtitioning defective (PAR) proteins into two distinct cortical domains. In this system polarity is maintained by the mutual inhibition between anterior (aPARs: PAR-3, PAR-6 and PKC-3) and posterior (pPARs: PAR-1, PAR-2 and LGL-1) PARs, which reciprocally antagonize their binding to the cortex, mutually excluding each other. We focused on LGL-1, which acts directly on PAR-6. Submitting LGL-1 to model membranes allowed us to identify a conserved region of the protein that binds negatively-charged membranes and to determine its lipid binding affinity and specificity. Selected LGL-1 mutants were then gen- erated to better understand the electrostatic mechanism involved in the membrane binding. LGL-1 was finally combined with PKC-3 to generate a functional membrane binding switch.

So what do you get when you combine the talents of two of the most famous Morses in rock (Neal of Spock's Beard & Transatlantic and Steve of Dixie Dregs, Kansas, & Deep Purple) and mix in some Mike Portnoy (Dream Theater) on drums, Dave LaRue (Dixie Dregs) on bass, and Casey McPherson (Alpha Rev) on guitar and vocals? A Prog super-group know as Flying Colors, of course! The first half of this week's show is dedicated to examining the two albums from said super-group. In part two, Tony and Dave give you a taste of a very interesting and different kind of project called Looking-Glass Lantern. The brain child of Graham Dunnington, a multi-instrumentalist with a PhD in Victorian Popular Music, LGL's specialty is Victorian/Prog adaptations of the adventures of Sir Arthur Conan Doyle's famous detective, Sherlock Holmes! Prog fans who also have an interest in classic literature will not want to miss this! Plus, we finally get to hear Dave's famous Steve Howe story! For more information on this week's artists, you can visit:http://www.flyingcolorsmusic.com/http://lookingglasslantern.com/As always, send questions, suggestions or comments to progsquatch@gmail.com. You can also follow the Squatch on Twitter (@ProgSquatch) or visit our Prog-Watch Facebook page.

Enterohämorrhagische E. coli (EHEC) gehören zu den wichtigen, lebensmittelassoziierten Erregern von Gastroenteritiden. Schwere Erkrankungsverläufe, wie die postinfektiöse Komplikation HUS sind bekannt und betreffen meist Kinder unter 5 Lebensjahren. Als Primärquelle gelten Wiederkäuer, in deren Gastrointestinaltrakt das natürliche Habitat von Shigatoxin-bildenden E. coli liegt. Über fäkale Kontamination von Lebensmitteln, Wasser oder auch direkten Kontakt können STEC oral vom Menschen aufgenommen werden, wobei nicht alle STEC gleich virulent sind. Manche, wie z. B. die der Serogruppen O26, O103, O111, O145 und O157 werden wesentlich häufiger bei erkrankten Menschen nachgewiesen als andere. Ziel dieser Studie war es zum einen fünf Singleplex Real-Time PCR-Systeme und ein Pentaplex Real-Time PCR-System zum Nachweis der oben genannten fünf E. coli-Serogruppen am LGL, Oberschleißheim, zu etablieren. Die in der Norm ISO/TS 13136:2012 als „hochpathogen“ eingestuften STEC-Stämme können somit anhand der dort beschriebenen Primer- und Sondensequenzen aus Probenisolaten detektiert werden. Für die Validierung wurden die Selektivität, Sensitivität, Präzision und Richtigkeit der PCR-Systeme bestimmt. Zum anderen wurden die Daten von 8272 humanen Proben (einschließlich der des EHEC O104:H4-Ausbruchs von 2011), 1521 Lebensmittelproben, 240 Tierkotproben, 69 Schlachtkörperproben und 29 Wasserproben aus den Jahren 2009 bis 2011 aus Bayern sowie die STEC-Serotypisierungsergebnisse von 09/2004 bis 12/2011 ausgewertet und beschrieben. Im Vergleich mit der gängigen Serotypisierung mittels Agglutination bietet das Real-Time PCR-Verfahren insbesondere bei großem Probenumfang einen enormen Zeitvorteil. Das Pentaplex PCR-System ermöglicht zudem eine zeitgleiche Analyse von Probenisolaten auf alle fünf Serogruppen. Alle E. coli-Stämme der Serogruppen O26, O103, O111, O145 und O157 wurden durch die PCR-Systeme korrekt nachgewiesen. Die O103-Sondensequenz wurde hierfür zuvor modifiziert. Die Spezifität der Nachweissysteme für O26, O103 und O145 lag in Bezug auf E. coli bei 100 %. Bei den Nachweissystemen für O111 und O157 zeigten auch Bakterienstämme anderer Gattungen ein positives Ergebnis in der PCR (Serratia entomophila / rfbEO157-positiv und Shigella sp. LGL 2869 / wbd1O111-positiv). Die Datenauswertung ergab unter anderem einen hohen Anteil stx-positiver Tierkot- und Schlachtkörperproben von Rindern. Die meisten stx-positiven Lebensmittel stammten von Wiederkäuern. Vereinzelt waren auch potenziell humanvirulente STEC nachzuweisen. Der Großteil der nicht-humanen Proben war weder eae- noch EhlyA-positiv, während nur ein Viertel der humanen Proben keines der beiden Gene aufwies. Bei humanen Proben dominiert die Gruppe der unter 1 bis 5-jährigen Erkrankten und Frauen sind häufiger betroffen als Männer. Die E. coli-Serogruppen O26, O103, O111, O145 und O157 gehören zu den häufigsten in Bayern und 2011 infizierten sich 47 Menschen in Bayern mit EHEC O104:H4.

In the more than 25 years since he discovered large granular lymphocyte – or LGL – leukemia, patients have traveled from all over the world to see Tom Loughran, M.D. of Penn State Hershey Cancer Institute. In this edition of Sound Health, Dr. Loughran discusses the discovery and his ongoing work to learn all he can about the disease. We also hear from one of his patients, Bob Meldrum, about why he travels from Colorado to be treated by Dr. Loughran. >> Read more about Dr. Loughran's LGL research in Penn State Medicine: The lifecycle of a cancer The finding — and following — of a killer (T-cell) Click on the Pod icon above or the direct link below the show. Right-click to save the file to your system.

Abstract Participation of Neospora caninum in abortions in cattle populations in Northern Bavaria Stated aim of this study was to investigate the incidence and the potential importance of Neospora caninum as a cause of abortion in cattle populations in Northern Bavaria. For this purpose the available diagnostic methods of the LGL in Erlangen were compared with each other relating to their efficiency. Aim was to find out which method or which combination of methods is most useful in the routine diagnosis. To serve this purpose, all abortion cases which were sent in for analysis to the LGL Erlangen (232 cases) within a year (2005-2006) were tested with different direct and indirect methods. Direct diagnosis of the infection was determined by histopathology, immunohistochemistry and PCR (polymerase chain reaction). ELISA (Enzyme Linked ImmunoSorbent Assay) and Western Blot were used for indirect analysis. 23 of the 232 cases were considered to be infected by at least one of the diagnostic techniques used. That implies that Neospora caninum is involved in 10% of abortions in Northern Bavaria. It’s impossible to prove beyond doubt if Neospora caninum is the main cause of abortions or an innovator for further infections. Suspicious cases could be identified by routine histopathology because of characteristic lesions – particularly in brain and heart. Typical histological lesions consisted overbalancing in multifocal nonsuppurative necrotizing encephalitis and non-purulent myocarditis. Most of these cases were confirmed by immunohistochemical staining with using a polyclonal antibody. Frequently low numbers of disease agents necessitate more (up to 4) immunohistochemical runs in some cases to verify the agent. Especially in cases of bold autolysis or mummified fetuses histology was applicable only with restrictons, Immunohistochemistry not at all. PCR is able to detect DNA of the parasit in these cases. Altogether the PCR got the most positive results. In cause of this three different techniques (conventionell PCR, Real Time SYBR Green, Real Time TaqMan) could adduce approximately same results. The Real Time TaqMan Technique gained slightly advantage over the two other methods. High costs and difficulties to take positive results are detriments of the PCR-method. There is no qualified statement about the importance of Neospora caninum as a cause of abortion, using the PCR method only. Therefore the PCR method should be combined with one additionally morphological method. Serological tests of Neospora caninum intrinsic antibodies in fetal fluids proved not to be very useful. Fetuses in early pregnancy develop partly no specific antibodies against Neospora caninum so that these cases were seronegative in spite of showing typical morphological lesions. Compared with this, 4 seropositive cases did not show any histopathological alterations. A final interpretation of such cases is impossible. Summarising this study confirms the importance of Neospora caninum as a cause of abortion in Northern Bavaria and underlines the need to use different diagnostic techniques to increase the chance to detect the infection in aborted fetuses. The best combination is histopathology with PCR. After a screening with histopathology, suspicious fetal tissues should be verified by PCR. Furthermore the PCR has got the highest sensitivity of all used methods. It is able to detect also cases without lesions although inspite of presenting the parasit. PCR was confirmed to be the best tool for the diagnosis of Neospora caninum in aborted fetuses.