Podcasts about cipc

South Africa's African National Congress (ANC) lost a legal bid to stop the MK party from using the name and logo of its former armed wing. The governing party had argued that uMkhonto we Sizwe (MK), headed by former President Jacob Zuma, had breached the trademark law. But the Durban High Court disagreed, allowing the use of the name, which translates as Spear of the Nation. It is a significant victory for the MK party ahead the 29 May general election. It ha been noted that neither the ANC, nor the MKP could prove to court that they could claim the trademark, because they had both failed to register it as a trademark. A search on the Companies and Intellectual Property Commission (CIPC) website proved that the logo trademark belonged to Legacy Projects. The CIPC is the legal custodian of trademarks and intellectual property. If youre asking what does all of this mean? Well this evening on Legal Matters, Marius Gerber, Intellectual Property Specialist at Witz Incorporated Inc., joins us to educate about how the Trade Marks Act, 1993 (Act 194 of 1993) says about trademarks and logos.See omnystudio.com/listener for privacy information.

Richard Frost, Head of Consulting at Armata SAfm Market Update - Podcasts and live stream

On this episode, Maui tackles a Motherboard-less PS5, Haitian cannibalism, Cape Independence, MK Party Threats, CIPC hack, Ryan Garcia, UKZN Imbalances, and a whole lot more! / Edit More: xoroyalty.net ℗ 2024 XO LUXURY GOODS

Jan Vermeulen, editor at Mybroadband on the chilling revelation of a ransomware group's claims to have infiltrated the Companies and Intellectual Property Commission (CIPC) since 2021, exposing the agency's alleged cover-up of a breach three years ago.      Peter Major, mining analyst on SA's platinum industry, warns of heightened pressures with significant job losses following a 35% plunge in PGM prices due to decreased global demand.   Hendrik Du Toit, CEO at Ninety One chats about the early stages of his career, to where he is now. And the asset management environment in the current tough economic climate we are in.See omnystudio.com/listener for privacy information.

Prof Basie von Solms, navorsingsprofessor in Kubersekuriteit by die Sentrum vir Kuberveiligheid aan die Universiteit van Johannesburg gesels oor die kuberaanval op die CIPC. Volg RSG Geldsake op Twitter

In a chilling revelation, a ransomware group claims to have infiltrated the Companies and Intellectual Property Commission (CIPC) since 2021, exposing the agency's alleged cover-up of a breach three years ago. The hackers assert control over the agency's systems, unveiling critical security flaws and demanding a $100,000 ransom. CIPC's response remains shrouded in secrecy as the attackers challenge the agency's commitment to cybersecurity, leaving sensitive information at risk. Jan Vermeulen, Editor at MyBroadband unpacks…See omnystudio.com/listener for privacy information.

Prof Basie von Solms van die Universiteit van Johannesburg gesels oor die kuberkrakers wat deur die CIPC se keerwal kraak. Volg RSG Geldsake op Twitter

In this episode, Kurt Thoennessen, CAPI, Founder and CEO of RiskRevu, discusses with Richard Kerr, CEO of Novatae, various challenges and solutions within the high net worth insurance space. Topics range from overcoming the hard market, networking strategies, the significance of quality submissions to underwriters, and the importance of specialization in the insurance industry. Kerr also delves into the foundation and goals of the Council for Insuring Private Clients (CIPC) and shares insights on the upcoming conference.Highlights:Challenges in High Net Worth Insurance Space: Discusses specific regional insurance challenges like finding hurricane coverage in Florida and fire coverage in California.Networking and Support: Emphasizes the importance of reaching out and networking within the industry, particularly through organizations like CIPC.Novatae's Strategy: Kerr details Novatae's approach in the insurance market, focusing on acquisitions and service improvement.Importance of Quality Submissions: Kerr stresses the need for agents to provide underwriters with clear and complete submissions to secure capacity.Council for Insuring Private Clients (CIPC): Discussion on the foundation and objectives of CIPC, highlighting its role in education, advocacy, and networking for insurance professionals.Upcoming Conference Details: The conference takes place May 1 and 2 in Dallas, TX at the Warwick hotel. Key themes include artificial intelligence in the high net worth space, capacity finding strategies, and building effective teams in the high net worth sector.Professional Development: Highlights the Certified Personal Risk Manager (CPRM) designation and its benefits for professionals in the industry.For more information or to register for the CIPC conference, visit the Council for Insuring Private Clients website: https://privateclientcouncil.com/. For more details about Novatae, visit their website: https://novatae.com/.For more details about the CPRM designation visit: https://www.scic.com/programs/cprm/

Jashwin Baijoo is the Head of Strategic Engagement and Compliance at Tax Consulting SA and joins Africa to speak on the new changes to the CIPC E-services system, that will affect all business owners.See omnystudio.com/listener for privacy information.

Joshua Alexandre – Founder, InfoDocs SAfm Market Update - Podcasts and live stream

Several local textile manufacturers have been using their poor black employees as fronts to achieve higher BEE scores to secure big contracts with major clothing retailers. The National Bargaining Council for the Clothing Manufacturing Industry, called for tighter legislation in addressing fronting practices that it says, undermine the country's laws. A blitz inspection in KwaZulu-Natal by officials from the bargaining council, led by the Department of Employment and Labour, uncovered widespread fronting in the textile and clothing manufacturing sector. The implicated companies were found to have registered their employees with the Companies and Intellectual Property Commission, CIPC, as directors, without the workers' knowledge and with no benefits.

"Ons het geweet hulle is verkeerd, maar ons kon niks doen nie" – Deonette de Ridder, uitvoerende hoof van Realcor Cape. Volg RSG Geldsake op Twitter

FNB's Wayne McCurrie on opportunities in the locally listed retail space as consumers continue to be squeezed. Fikile Mbhokota from Satrix on 30 years of ETFs in the US and 23 locally. Joshua Alexandre from InfoDocs on new requirements for all companies to submit beneficial ownership information to the CIPC.

Guest: Dennis Bloem, COPE SpokespersonSee omnystudio.com/listener for privacy information.

The winner of the tamper is announced in this weeks episode and we also talk about pens and CIPC.  Thats all I remember at the moment, I guess you should just listen.  The word Assclown makes an appearance in this episode, it is one of my favorites.  Also, it was Robert Oyster's 7th birthday, but who cares. Enjoy. Support the show Questions or comments? Freel fee to submit them to our facebook group: https://www.facebook.com/groups/1247125679194697or on the Discord server at:https://discord.gg/PkMqe74dYnor simply email:greywoodie@gmail.com All your base are belong to us

Nzuzo provides a detailed but different opinion on the judgement of the “Kill the Boer” song and Dumo provides an update on his trademark application for the term Coerso-Vaxxer™ with the CIPC. SIGN UP TO OUR NEWSLETTER · WATCH THIS EPISODE

Cuma Zwane – ondersoeker, CIPC

Herman Lombaard – onafhanklike makerlaar

On the 16th episode of Immigration Today! Angeline Chen interviews Shiu-Ming Cheer. Shiu-Ming is the Deputy Director of Programs and Campaigns at the California Immigrant Policy Center. They are a constituent-based, statewide immigrant rights organization that promotes and protects safety, health and public benefits and integration programs for immigrants. They are one of the few organizations that effectively combines legislative and policy advocacy, strategic communications, organizing and capacity building to pursue its mission. Shiu-Ming graduated from U.C. Berkeley and from the Epstein Program in Public Interest Law and Policy at UCLA School of Law.  Shiu-Ming has received awards for her community and legal work from the National Lawyers Guild – LA and the Long Beach Immigrant Rights Coalition.In this podcast, Shiu-Ming goes into detail about how she became involved in immigrant rights as a young college graduate. She migrated to America at the young age of 2 and has over 25 years of experience in immigrant rights and social justice issues. The California Immigrant Policy Center came about because of draconian anti-immigration laws in 1996 that made it impossible for immigrants to access public health benefits. The Center has had victories such as playing a huge role in securing driver's licenses for everyone regardless of immigration status. They also pushed for the CA Values Act in 2017 (SB 54) which prevents state and local law enforcement agencies from using their resources on behalf of federal immigration enforcement agencies. All of this has been influential to the progression of support that other states begin to show toward immigrants.If you are listening to this episode, you can make a difference in the way these policies play out at your local level. If you are a registered voter or simply a citizen in your city, you have the power to call on your local and state representatives and urge them to support policies that protect immigrants. You can also engage any organization that accepts volunteers for direct action such as the California Immigration Policy Center itself. Please follow CIPC on Instagram, Facebook and Twitter. Most importantly, consider donating here or sign up for the email alerts on their webpage.Immigration Today! is always releasing new content. Please subscribe to our newsletter to stay up to date with new episodes!DISCLAIMER – No information contained in this Podcast or on this Website shall constitute financial, investment, legal and/or other professional advice and that no professional relationship of any kind is created between you and podcast host, the guests or Clark Hill PLC. You are urged to speak with your financial, investment, or legal advisors before making any investment or legal decisions.

What if you were crystal clear with your money? What if you changed your outlook on Money? Tune it to listen on how to gain clarity with money.   Book a Call Today with Jennifer:   https://www.jenniferjakobsenlifecoaching.com/contact     IG: Jennifer Jakobsen, MSW, CIPC (@jjakobsenlifecoach) •   Clarity to Cash on Apple Podcasts

Cuma Zwane – ondersoeker, CIPC

In this episode, John and the gang discuss the wild west of cryptocurrencies and NFT's (Non-Fungible Tokens). Several scams are out there that can really put a hurting on your accounts and livelihood. Be careful out there when you are investing to ensure that you are getting the best bang for your buck and ensuring that your money is not going to something illegal or into a scam. We want to know more about your situations so we can create better tailored content. John and the team can be reached at jcindia@lifestagesadvisory.com. Reach out to us so we can get you or your segment featured on the show!

Jennifer Jakobsen helps mom entrepreneurs who are struggling with guilt, overwhelm, and then lack of balance launch and/or grow a successful business while having fun in the process. She is the Mompreneur coach for feeling amazing in Business and Mom life! Connect with Jennifer! Jennifer Jakobsen - Helping Mompreneurs have ultimate success in life and business. (jenniferjakobsenlifecoaching.com) Jennifer Jakobsen, MSW, CIPC (@jjakobsenlifecoach) • Instagram photos and videos (17) Jennifer Jakobsen Life Coaching | Facebook

Maleic hydrazide is a growth regulator that has been on the market for many years. The true value of it is now becoming apparent, with the demise of chlorpropham (CIPC). Previously it was used mainly for volunteer control. Nowadays it is especially important in storage. Here it can provide sprout control as a standalone product, or as a cost-saving foundation for other, post-harvest treatments. Maleic hydrazide is a foliar treatment that needs to be translocated to the tubers. Successful treatments are therefore dependent on plant, weather and application conditions.  This podcast talks about some of those conditions and introduces a webinar on maleic hydrazide, that will take place on June 15th.  Speakers: Adrian Briddon - Crop Storage Senior Scientist at Sutton Bridge Andrew Webster - Potato grower Learn more about storage: https://ahdb.org.uk/storage-hub  Sign-up for the storage webinar: https://ahdb.org.uk/events/maleic-hydrazide-a-foundation-for-potato-storage Feedback survey: We'd love to know what you think of our podcasts. If you'd like to give your feedback, please complete our short questionnaire, which will help us to improve the podcast on an ongoing basis.   Podcast produced and edited by Amna Yusaf - Marketing and Communications Executive 

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On this episode, Lawrence LeClair the Director of Government Relations at NASBP, and Michael Oscar the Director of Government Relations at the American Subcontractors Association (ASA) joins us to discuss the Construction Industry Procurement Coalition (CIPC). We discuss the importance of coalitions, the founding of CIPC, its successes and future legislative, regulatory and congressional matters. Find out about NASBP’s Governments Relations activities and resources as well as ASA’s Government Relations activities and resources. Hosted by: Kat Shamapande, Director, Professional Development, NASBP and Mark McCallum, CEO, NASBP

Jennifer helps mothers feel confident and happy in motherhood. She’s a mom of 3 drama queens, MSW and CIPC certified. --- Send in a voice message: https://anchor.fm/momstalkingshit/message Support this podcast: https://anchor.fm/momstalkingshit/support

In this episode we are discussing how potato growers should prepare their crops and stores for the first season without CIPC. Guest speakers: Tom Macfarlane - Farm Manager at Bannister Farms James Lee - Business Unit Manager at Produce Solutions Adrian Cunnington - Head of Crop Storage Research at Sutton Bridge Links:   Learn more about storage without CIPC  (https://ahdb.org.uk/storage-hub) Watch Store Cleaning Webinar ( https://www.youtube.com/watch?v=bB96VBh16hQ ) Book a free one-to-one potato store visit via our new Storage Network (https://ahdb.org.uk/storage-network) Register to attend desiccation webinars: 19 August (https://attendee.gotowebinar.com/register/1725535474264091920?source=Podcast) : Desiccation - how to apply diquat alternatives to potato crops 16 September (https://attendee.gotowebinar.com/register/938151008358548493?source=Podcast) : Desiccation - how effective are diquat alternatives for potato crops

In the first season without CIPC we are looking at alternative sprout suppressant solutions. In this episode we will discuss more about Maleic Hydrazide, including Sutton Bridge trials, instructions on how this chemical should be applied as well as costs of application.  Speakers: Jonathan Papworth- Potato grower in Cambridgeshire and AHDB Potatoes Board member   Adrian Briddon - AHDB Crop Storage Senior Scientist Andy Alexander - Independent Agronomist and Potato Specialist Useful links: https://ahdb.org.uk/knowledge-library/maleic-hydrazide-as-a-potato-sprout-suppressant https://ahdb.org.uk/storage-hub

In this episode we are focusing on the decisions you will have to make before you plant, to make sure you will be able to desiccate without diquat and store without CIPC.    Sequence of discussions and speakers:   Desiccation talk with Will Gagg (SPot Farm North host)   Knowledge Exchange team’s view on desiccation – Bill Watts, David Wilson and Harry Henderson  Advice on what you can do now to prepare for storing without CIPC – Adrian Cunnington (Sutton Bridge - Head of Crop Storage Research)   Links:    http://www.ahdb.org.uk/news/the-cost-of-desiccation-without-diquat   https://ahdb.org.uk/storage-hub 

Policiais da Companhia Independente de Policiamento com Cães (CIPCães) da Policia Militar do Amazonas são homenageados na Assembleia legislativa do Amazonas.

The African Centre for Migration and Society says foreigners are likely to make their way back into the Johannesburg CBD in hopes of earning a living. This after the Johannesburg Magistrates Court ordered the deportation of dozens of foreign nationals who were arrested during a counterfeit goods raid in the city centre last week. The court says those scheduled for deportation within 30-days will, in the meantime, be moved to the Lindela Repatriation Centre in Krugersdorp.

This week on Mimosas with Moms, Abbey and Ashleigh welcome Jennifer Jakobsen, MSW, CIPC! Jennifer is a life coach who specializes in helping moms. She shares how she helps moms discover what’s important, eliminate stress, and gain more confidence in their parenting! Want to be the best mom you can be? Let’s talk about it, CHEERS!! ________________________________ Jennifer Jakobsen, MSW, CIPC https://www.jenniferjakobsenlifecoaching.com/home Instagram @jjakobsenlifecoach Email jakobsenlifecoaching@gmail.com ________________________________ Instagram @mimosaswithmoms Like our FB page https://m.facebook.com/Mimosas-with-Moms-2329402587291677/ Email 2mimosamoms@gmail.com

In the episode; AHDB's Laura Bouvet and Adrian Cunnington of Sutton Bridge Crop Storage research discuss the impact of losing CIPC could have on the potato industry. You can find out more information on this by visiting our Storage Hub (https://ahdb.org.uk/storage-hub)

Show Notes: Sun ('21) hosts the show this week! Info on scheduling, events, and this week's need-to-knows. Yeon Seo ('21) and Janet ('21) bring the latest updates from our Mighty Dolphins. Sparsh ('20) and Jessica ('20) stop by the studio to chat about the CIPC program and what to do about anxiety. As usual, our upper school birthday announcements. Find us Online https://sites.google.com/chadwickschool.org/thisweekatwick/ @twawick on Instagram and Twitter Subscribe via your favorite podcast app. --- Send in a voice message: https://anchor.fm/this-week-at-wick/message

The CIPC has decided to change the manner in which annual returns are submitted by practitioners in order to ensure uniformity and standardization. The multitude of various submissions is to minimize with the introduction of the concept of reporting via Extensible Business Reporting Language (XBRL). From what has been communicated, XBRL is a web-based computer language that is intended for business reporting and allows account numbers to be tagged within XBRL format, using predefined descriptions. This standardized tagging improves analysis of financial information and leads to more comparable and reliable information. SAIPA's Mrs. Faith Ngwenya, who is the Executive for Technical and Standards unpacks this further. --- This episode is sponsored by · Anchor: The easiest way to make a podcast. https://anchor.fm/app Support this podcast: https://anchor.fm/the-south-african-institute-of-professional-accountants/support

Business Day TV — Warren Bonheim, CCO from Zinia joins Alan in this week's edition of Psyche of Success, an incredible story of somebody that goes from zero to hero and shares his experiences in what not to do when your business is doing well. Joe Mathekga from CIPC and Juanita Steenekamp from SAICA about format changes to the submission of annual financial statements to CIPC which will take effect from 2018

Business Rescue was introduced by the new Companies Act (71 of 2008) in order to reduce the number of companies filing for bankruptcy. SAIPA (South African Institute of Professional Accountants) has been accredited by the CIPC as one of the first 2 professional organisations to regulate its members to be Business Rescue Practitioners. Prof Rashied Small, Executive of Education, Membership and Training at SAIPA joins me to discuss what exactly is Business Rescue and how SAIPA its members will be involved in this process. --- This episode is sponsored by · Anchor: The easiest way to make a podcast. https://anchor.fm/app

World Bank implicated in the Panama Papers leak said to be investing in companies linked to tax havens as reported by Oxfam, Ghana offers Nigeria $6bn infrastructure loan, Nedbank and CIPC team up for online service, World Bank reports that Low commodity prices will continue to negatively impact growth in Africa, Former Governor of The Central Bank of Kenya to be problem over banking crisis, South Sudan rebel deputy arrives in Juba, Bank of Ghana to fight inflation and boost growth, Kenya to fight fraud within the Insurance sector etc --- Send in a voice message: https://anchor.fm/africabusinessnews/message Support this podcast: https://anchor.fm/africabusinessnews/support

World Bank implicated in the Panama Papers leak said to be investing in companies linked to tax havens as reported by Oxfam, Ghana offers Nigeria $6bn infrastructure loan, Nedbank and CIPC team up for online service, World Bank reports that Low commodity prices will continue to negatively impact growth in Africa, Former Governor of The Central Bank of Kenya to be problem over banking crisis, South Sudan rebel deputy arrives in Juba, Bank of Ghana to fight inflation and boost growth, Kenya to fight fraud within the Insurance sector etc --- This episode is sponsored by · Anchor: The easiest way to make a podcast. https://anchor.fm/app Support this podcast: https://anchor.fm/africanperspective/support

World Bank implicated in the Panama Papers leak said to be investing in companies linked to tax havens as reported by Oxfam, Ghana offers Nigeria $6bn infrastructure loan, Nedbank and CIPC team up for online service, World Bank reports that Low commodity prices will continue to negatively impact growth in Africa, Former Governor of The Central Bank of Kenya to be problem over banking crisis, South Sudan rebel deputy arrives in Juba, Bank of Ghana to fight inflation and boost growth, Kenya to fight fraud within the Insurance sector etc --- This episode is sponsored by · Anchor: The easiest way to make a podcast. https://anchor.fm/app

Vor Beginn dieser Arbeit war ein A. fumigatus-Protein (Chp: CipC homologes Protein) mit unbekannter Funktion und hoher Homologie zum CipC-Protein aus A. nidulans als prominentes hyphenspezifisches Protein identifiziert worden (Schwienbacher, 2005). Weiterhin gab es zu diesem Zeitpunkt Hinweise, dass ein zu CipC homologes Protein in C. neoformans eine wichtige Rolle während der Virulenz spielt (Steen et al., 2003). In dieser Arbeit sollte die biologische Funktion des pilzspezifischen Proteins genauer untersucht werden. Zu diesem Zweck wurden monoklonale Antikörper gegen Chp, mehrere Reporterstämme sowie eine Deletionsmutante hergestellt. Die erhobenen Daten zeigen, dass Chp als Monomer im Cytosol der Hyphen vorliegt. Dabei zeigte sich eine gleichmäßige Verteilung eines GFP-Fusionsproteins innerhalb der Hyphen; nur die Vakuolen schienen ausgespart. Die Identifikation des Proteins auf der Sporenoberfläche von A. fumigatus (Asif et al., 2006) wurde wiederlegt und die differentielle Expression des Proteins bestätigt. Anders als in A. nidulans (Melin et al., 2002) wirkt das Antibiotikum Concanamycin A auf die Bildung von Chp in A. fumigatus nicht induzierend. Da diese Tatsache sowohl für die Na-mensgebung von CipC, als auch für die Namensgebung von Chp verantwortlich war, sollte das A. fumigatus-Protein umbenannt werden. Die Wahl des Namens fiel auf NrpA (Nitrogen regulated protein A), da die Bildung des Proteins von der N-Quelle abhängig ist. Die N-abhängige Regulation war für ein homologes F. fujikuroi Gen auf RNA-Ebene bereits bekannt (Teichert et al., 2004). In der vorliegenden Arbeit konnte sie in A. fumigatus und erstmals auf Proteinebene bestätigt werden. Desweiteren wurden auch neue N-Quellen untersucht. Dabei zeigte sich, dass NrpA in Anwesenheit der N-Quellen Glutamat, Nitrat oder Harnstoff nicht gebildet wird, wohin-gegen Komplexmedien sowie die N-Quellen Ammonium, Glutamin, Asparaginsäure, Asparagin, Valin und Tryptophan zur Bildung des Proteins führen. In Kombination einer induzierenden und einer unterdrückenden N-Quelle dominiert stets die induzierende. In Reporterstämmen (gfp; lacZ) fand diese negative Regulation der Bildung von NrpA nicht statt. Das Protein wurde sowohl in Anwesenheit einer normalerweise unterdrückenden N-Quelle, als auch in den Sporen gebildet. Weiterhin nimmt die gebildete Menge von NrpA sowohl bei längeren Inkubationszeiten, als auch bei Verwendung höherer Animpfdichten zu. Wird der Pilz zunächst mit einer die NrpA-Bildung unterdrückenden N-Quelle angezogen und dann in Medium mit einer induzierenden N-Quelle umgesetzt, dauert es 6 h bis eine Bildung von NrpA verzeichnet werden kann. Diese Zeitspanne blieb auch in einem inversen Experiment gleich. Als nächstes wurde untersucht, ob NrpA in A. fumigatus unter Stressbedingungen von Bedeutung ist. Dabei konnte gezeigt werden, dass sowohl osmotischer Stress, als auch oxidativer Stress, der durch Menadion verursacht wird, kei-ne Auswirkung auf die gebildete NrpA-Menge hat. Dagegen führt durch H2O2 verursachter Stress zu einem veränderten Laufverhalten von NrpA in SDS-Gelen. Das Protein scheint unter diesen Umständen ein höheres Molekulargewicht zu haben. Mithilfe eines A. fumigatus-Reporterstammes, der ein NrpA-GFP-Fusionsprotein bildet, konnte gezeigt werden, dass H2O2 auch zu einer veränderten Lokalisation von NrpA führt. Das sonst gleichmäßig in den Hyphen verteilte Protein formierte sich in punktförmigen Strukturen. Auch unter Mangelbedingungen spielt NrpA keine wichtige Rolle, denn weder ein C- noch ein N-Mangel verändert die gebildete Menge des Proteins. Dient die normalerweise die NrpA-Bildung induzierende N-Quelle Glutamin als C- und N-Quelle wird NrpA nicht gebildet. Ebenso wie in F. fujikuroi (Teichert et al., 2002) wird die Bildung des NrpA-Proteins durch MSX, einem Inhibitor der Glutaminsynthetase, fast vollständig inhibiert. Anders als in F. fujikuroi (Teichert et al., 2006) verursachte die Inhibierung der TOR-Kinase durch Rapamycin keinen Effekt auf die Bildung von NrpA. Auch durch eine her-gestellte Deletionsmutante konnte die biologische Funktion von NrpA nicht geklärt werden. In zahlreichen vergleichenden Untersuchungen verhielt sich die Mutante ebenso wie der Wildtyp. Der einzige dokumentierte Unterschied zwischen Mutante und Wildtyp ist eine verstärkte Bil-dung der Katalase 1 in der Deletionsmutante. Anders als angenommen spielt NrpA während der Virulenz von A. fumigatus keine Rolle. In einem Virulenzmodell in embryonierten Hühnereiern verhielten sich Deletionsmutante und Wildtyp gleich. Auch in murinen Makrophagen führten die Deletionsmutante und der Wildtyp etwa zu vergleichbaren Mengen an ausgeschüttetem IL-10 und TNFα. Ein potentieller Nutzen von NrpA bei der Diagnose der allergischen bronchoalveolaren Aspergillose (ABPA) konnte ebenso ausgeschlossen werden. Neben NrpA waren im Vergleich der Proteinmuster der verschiedenen A. fumigatus-Morphotypen auch weitere differentiell exprimierte Proteine aufgefallen. Eines davon war eine MnSOD (Aspf6), die bis dahin auch als mitochondriale MnSOD bezeichnet wurde (Rementeria et al., 2007). Da im Genom von A. fumigatus aber eine weitere MnSOD kodiert ist, die über eine putative Mitochondriensignalsequenz verfügt, sollte in einem zweiten Teil der Arbeit die tat-sächliche Lokalisation dieses Proteins gezeigt werden. Dafür wurden zunächst monoklonale Antikörper gegen das Protein hergestellt. Eine mitochondriale Lokalisation der MnSOD mit puta-tiver Signalsequenz konnte gezeigt werden. Dabei wurden sowohl Western-Blots als auch ein A. fumigatus-GFP-Reporterstamm verwendet. Weiterhin konnte das Protein genauer charakteri-siert werden. Im Gegensatz zu Aspf6, das nur in den Hyphen des Pilzes zu finden ist, wurde die mitochondriale MnSOD sowohl in den Sporen, als auch in den Hyphen nachgewiesen. Die gebil-dete Menge des Proteins verändert sich im Zeitverlauf nicht. Lediglich zu sehr späten Wachs-tumszeitpunkten in der späten stationären Phase war ein leichter Anstieg zu beobachten. Die gebildete Menge des Proteins hing auch nicht von der Animpfdichte der Kultur ab. Anders als Aspf6, das bekannterweise ein Homotetramer bildet (Flückiger et al., 2002), scheint die mitochondriale MnSOD als Dimer vorzuliegen. Auch in Antwort auf oxidativen Stress verhielten sich die beiden MnSODs unterschiedlich. Menadion, das innerhalb der Zelle die Bildung von Su-peroxidanionen bewirkt, führte zu einem leichten Anstieg der Proteinmenge der mitochondrialen MnSOD, während Aspf6 unverändert blieb. Als Folge von oxidativem Stress, der durch H2O2 verursacht wird, zeigte Aspf6 eine leichte Verringerung, während die mitochondriale MnSOD schnell abgebaut wird. In einem dritten Teil dieser Arbeit wurde die Rolle der Atmung während der Auskeimung von A. fumigatus genauer untersucht. Dabei konnte gezeigt werden, dass die Proteinbiosynthese für den Auskeimungsprozess unbedingt notwendig ist. Desweiteren wurde mit Hilfe unterschiedli-cher Methoden eine sehr frühe Aktivierung der Atmungskette während des Auskeimungspro-zesses nachgewiesen. Ebenso konnte gezeigt werden, dass die Anwesenheit von Sauerstoff für das Wachstum von A. fumigatus unbedingt erforderlich ist. Im anaeroben Milieu konnten die Konidien weder anschwellen noch auskeimen. Auch bereits vorhandene Hyphen konnten unter Abwesenheit von Sauerstoff nicht weiterwachsen. Weitere Untersuchungen zeigten, dass A. fu-migatus anders als A. nidulans (Takasaki et al., 2004) nicht über die Fähigkeit verfügt, im Anae-roben durch eine Fermentation von Ammonium zu überleben. In dieser Arbeit wurde ebenso wie in anderen aktuellen Arbeiten (Williger et al., 2008) die Fähigkeit von A. fumigatus, in hypoxischen Umgebungen zu wachsen, nachgewiesen.