Podcasts about gtps

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Best podcasts about gtps

Latest podcast episodes about gtps

Red Mist Podcast
S3 Ep45: F1 in Vegas Preview

Red Mist Podcast

Play Episode Listen Later Nov 20, 2024 50:37


Welcome to our motorsports podcast where we discuss F1, IndyCar, IMSA, NASCAR, our own racing adventures, and some other adventures!Formula 1 picks back up this weekend with a sprint to the season end in the form of a triple header. First up is the Strip in Vegas. We'll see if Lando can keep the championship alive or if Max can clinch it.We discuss some other news and racing from this past weekend including NASCAR driver announcements, drivers testing Indycars and GTPs, along with a recap of the Macau Grand Prix and the GT World Cup race.We would like to congratulate all of the SKUSA Supernats winners as well as everyone else who was able to attend the event in Vegas!Coming up this weekend: Formula 1 is at Vegas

Physio Edge podcast
165. Busting hip pain myths with Mehmet Gem

Physio Edge podcast

Play Episode Listen Later Nov 5, 2024 68:35


Join hosts David Pope and The Hip Physio - Mehmet Gem as they dive deep into the myths and misconceptions surrounding hip and groin pain. This podcast explores common myths about glutes and hip flexors, effective rehab exercises, and how to accurately assess and treat various hip conditions. Get free access to the "Tricky tendons" infographic series Unlock the secrets of successful tendinopathy assessment and treatment with this free infographic series for therapists. Links Improve your musculoskeletal and sports injury assessment & treatment results with a free trial Clinical Edge membership LEAP Trial Click here to register and receive immediate access to the Tricky tendons infographic series Free Achilles tendinopathy video series with Tom Goom David Pope at Clinical Edge Download and subscribe to the podcast on iTunes Download the podcast in Overcast Listen to the podcast on Spotify Mehmet Gem David Pope on Twitter David Pope & why I started Clinical Edge Review the podcast on iTunes Infographics by Clinical Edge Chapter markes: 00:00 Intro 00:27 Getting to know Mehmet 03:11 Glute myths and misconceptions - Can patients have "gluteal amnesia" or "switched off glutes"? 08:54 When and how to test & improve glute strength 12:58 Functional glute testing 14:49 Mehmet's favourite glute exercises 19:22 Greater trochanteric pain syndrome/gluteal tendinopathy 23:04 Stretches for lateral hip pain? 25:52 Should patients perform hip mobility exercises? 29:07 Improving hip range of movement 36:48 When to test hip range of movement 39:40 Differentiating hip osteoarthritis (OA) from GTPS 44:24 Hip objective assessment - OA vs GTPS 46:51 GTPS diagnosis 49:44 Return to running with hip dysplasia 52:37 Hip flexors - what are they responsible for? 53:42 Do hip flexors get tight from sitting? 54:39 Hip flexors - objective assessment 57:43 Hip rehab exercises  

Red Mist Podcast
S3 Ep28: Hot Weekend of Racing

Red Mist Podcast

Play Episode Listen Later Jul 17, 2024 69:03


Welcome to our motorsports podcast where we discuss F1, IndyCar, IMSA, NASCAR, our own racing adventures, and some other adventures!Indycar had its doubleheader at Iowa Speedway and it was a good heat test for the new hybrids as temperatures were high 80s to low 90s for both races. The repave changed the racing which the drivers had to navigate and pit stops had to be perfect as they played a more crucial role.NASCAR was at Pocono where Ryan Blaney took the win. WEC returned to Interlagos for the first time in 10 years and what a show they put on! Fantastic battles throughout the race! Great return to a great facility for these cars. Hope they keep this track around in future seasons. IMSA had another round at Mosport with the LMP2s being the top class. Both GT classes were there as well so just the GTPs that took a round off.Coming up this weekend: F1 is at Hungary, NASCAR returns to Indy for the Brickyard 400, and Indycar is on the streets of Toronto.

That Podcast with Sami Rye
171 - Moving is Hell and fun with Creating AI GTPs

That Podcast with Sami Rye

Play Episode Listen Later Jul 10, 2024 38:44


This week I am moving and I will share some of that along with some incredible features I did not realize ChatGTP has that can change everything you are doing

Red Mist Podcast
S3 Ep12: Jake Innes & Sebring Recap

Red Mist Podcast

Play Episode Listen Later Mar 20, 2024 57:16


Welcome to our motorsports podcast where we discuss F1, Indycar, IMSA, NASCAR, our own racing adventures, and some other adventures!A good friend of the podcast Jake Innes joins us to discuss his young racing career. Jake comes from Long Island where he takes part in karting leagues at Grand Prix New York and also takes part in the Mini Stock class at Riverhead Raceway. We also discuss his trip last month to the Daytona 500.We recap the racing from NASCAR at Bristol and IMSA at Sebring (all the series) from this past weekend. The Bristol race was a strategy filled race as tires were fading and fading fast. Sebring had great battles throughout all the series that went there. From MX-5s all the way up to the GTPs in the WeatherTech series.Coming up this weekend NASCAR is at COTA, F1 and Supercars are at Melbourne, Indycar has it's exhibition race at Thermal, and MotoGP is running at Portimao.

SehnenStark
#6 Wütende Kugelfische & Gluteale Tendinopathien mit Felix Kade Teil 2

SehnenStark

Play Episode Listen Later Mar 12, 2024 37:41


Teil 2 der Folge mit Felix Kade. Da du Teil 1 gehört hast, weißt du mittlerweile, was eine Gluteale Tendinopathie ist.Aber welche Rolle spielt der Alltag bei dieser Schmerzthematik mit rein und welche Muster können die Schmerzen auslösen?Welche Ausprägungen können die Symptome haben und gibt es andere Überlastungen/Verletzungen oder sogar Krankheiten, die das ganze verschleiern können?All das bespricht Nils mit Felix Kade.Felix war selbst stark von einer Glutealen Tendinopathie betroffen und hilft Menschen, die daran leiden, die Schmerzen los zu werden.Dafür hat er einen unfassbar guten Online Kurs produziert, den ich mir im Vorfeld anschauen durfte und den ich sehr empfehlen kann, wenn man selbst an einer Glutealen Tendinopathie leidet.Den Kurs kannst du hier kaufen:https://elopage.com/s/felixkade/onlinekurs-gtps-gluteale-tendinopathieAußerdem findest du super viele nützliche Artikel auf seiner Homepage unter:https://www.felixkade.de/Hat dir etwas gefehlt, was wir vergessen haben? Oder du möchtest uns generelles Feedback zum Podcast geben? Dann schreib uns eine Nachricht bei Instagram an @nils.heim.schmerzcoach oder per Mail an coaching@nilsheim.deWenn du selbst von Patella- oder Achillessehnen Schmerzen betroffen bist und diese endlich los werden möchtest, dann sichere dir jetzt einen Termin für ein kostenloses Vorgespräch, in dem wir darüber sprechen, ob wir dir weiterhelfen können.Hier kostenlosen Termin buchen:https://calendly.com/nh-schmerzcoaching/kostenloses-vorgesprach

SehnenStark
#5 Wütende Kugelfische & Gluteale Tendinopathien mit Felix Kade Teil 1

SehnenStark

Play Episode Listen Later Mar 6, 2024 47:08


Was haben Achillessehnen Schmerzen und Schmerzen an der Außenseite der Hüfte gemeinsam?Ist wirklich eine Schleimbeutelentzündung das Problem, wenn man Schmerzen an der Außenseite der Hüfte hat oder sollten wir nicht lieber Gluteale Tendinopathie sagen?Und was haben wütende Kugelfische mit all dem zu tun?Diese Fragen und noch vieles mehr bespricht Nils mit dem ersten Gast im SehnenStark Podcast: Felix KadeFelix war selbst stark von einer Glutealen Tendinopathie betroffen und hilft Menschen, die daran leiden, die Schmerzen los zu werden.Dafür hat er einen unfassbar guten Online Kurs produziert, den ich mir im Vorfeld anschauen durfte und den ich sehr empfehlen kann, wenn man selbst an einer Glutealen Tendinopathie leidet.Den Kurs kannst du hier kaufen:https://elopage.com/s/felixkade/onlinekurs-gtps-gluteale-tendinopathieHat dir etwas gefehlt, was wir vergessen haben? Oder du möchtest uns generelles Feedback zum Podcast geben? Dann schreib uns eine Nachricht bei Instagram an @nils.heim.schmerzcoach oder per Mail an coaching@nilsheim.deWenn du selbst von Patella- oder Achillessehnen Schmerzen betroffen bist und diese endlich los werden möchtest, dann sichere dir jetzt einen Termin für ein kostenloses Vorgespräch, in dem wir darüber sprechen, ob wir dir weiterhelfen können.Hier kostenlosen Termin buchen:https://calendly.com/nh-schmerzcoaching/kostenloses-vorgesprach

GPnotebook Podcast
Ep 91 – Lateral hip pain

GPnotebook Podcast

Play Episode Listen Later Feb 8, 2024 16:09


Lateral hip pain is a common presenting complaint in primary care. It can result in significant morbidity with reduction in activity levels and associated implications for leisure activities, employment and overall wellbeing. In this episode, Dr Kate Chesterman reviews the likely differential diagnoses, including some key questions to consider when assessing a patient with lateral hip pain. She then delves deeper into the diagnosis of greater trochanteric pain syndrome (GTPS), exploring the reasons why it is no longer referred to as trochanteric bursitis, initial management options, the value of corticosteroid injections and indications for onward referral.

The Marshall Pruett Podcast
MP 1487: The Week In IndyCar Intv with Scott McLaughlin, Feb 5 2024

The Marshall Pruett Podcast

Play Episode Listen Later Feb 6, 2024 21:41


It's The Week In IndyCar interview show featuring Team Penske's Scott McLaughlin. TOPICS: Title contention in the new season, racing at Daytona in IMSA LMP2, interest in racing Porsche 963 GTPs, starting a new fan experience package, shared disappointment in the movie Gran Turismo, and more! NEW show stickers: ThePruettStore.com EVERY episode is graciously supported by Pfaff Technologies, the Justice Brothers, and TorontoMotorsports.com. If you'd like to join the PrueDay podcast listener group, send an email to pruedayrocks@gmail.com and you'll be invited to participate in the Discord chat that takes place every day and meet up with your new family at IndyCar events. Play on Podbean.com: https://marshallpruett.podbean.com/ Subscribe: https://marshallpruettpodcast.com/subscribe Join our Facebook Group: https://www.facebook.com/MarshallPruettPodcast [WTI]

Reptile Talk
Episode ONE HUNDRED NINETEEN - Aiden Bradley (SoCal Biawaks)

Reptile Talk

Play Episode Listen Later Jan 25, 2024 61:40


Super stoked to talk with Aiden this week!!  From captive bred Dumeril's Monitors to GTPs, Field Herping and more!!!  It's gonna be a jam packed episode!!  Don't miss it!!  Check Aiden out on IGhttps://www.instagram.com/socalbiawak...Reptile Talk is Sponsored by:BLACK BOX CAGES**USE CODE - REPTILETALK and SAVE on your next order!!! **Website:https://bit.ly/BLKBoxIG:https://bit.ly/BLKBOXIGFacebook:https://bit.ly/BLKBOXFBFOLLOW USARKUSARK.orgIG - @usark_officialFB - United States Association of Reptile KeepersFIND US ON IGhttps://bit.ly/ReptileTalkIGhttps://bit.ly/creepingitrealIGhttps://bit.ly/BMGIGFIND US ON FBhttps://bit.ly/ReptiletalkFB

Radar Agro
Duas mulheres da pecuária na COP28 | Fala Carlão

Radar Agro

Play Episode Listen Later Dec 4, 2023 7:24


Fala Carlão conversa com Luiza Bruscato, Diretora Executiva da Mesa Brasileira de Pecuária Sustentável (GTPS) e com a executiva Sheila Guebara, Diretora da Seara e Líder de Sustentabilidade da JBS. Uma prosa com duas mulheres da prateleira de cima da pecuária brasileira. Gente que sabe o que fala. Falem à vontade, meninas! Conheça nossos apoiadores: Datagro Markets - https://portal.datagro.com/auto-register/ Ubyfol - https://ubyfol.com/ Grupo Publique - https://grupopublique.com.br/ AgroRevenda - https://agrorevenda.com.br/ Assista outra entrevista relacionada: 1: https://www.youtube.com/watch?v=dkBYx7aG3U0

2024
Arrivano i GTPs - Fernride e guida ibrida - BYD - Meta a pagamento - Rapporto Clusit

2024

Play Episode Listen Later Nov 10, 2023


OpenAI lancia le GPTs, varianti di ChatGPT che gli utenti possono personalizzare e che aprono nuove prospettive all'uso dei sistemi di intelligenza artificiale generativa come spiega Enrico Bertino, Chief AI Officer della piattaforma di intelligenza artificiale "Indigo.ai".Con Gabriele Faggioli, presidente del Clusit, Associazione italiana per la sicurezza informatica, parliamo dei dati emersi dall’aggiornamento del rapporto sulla cybersecurity che a livello globale registra quasi 1400 attacchi gravi nei primi sei mesi dell’anno.Mentre si aggrava la crisi per Cruise, l’azienda di proprietà di GM specializzata in guida autonoma, parliamo di trasporti con tecnologia di guida ibrida autonoma/remota con Hendrik Kramer, Ceo di Fernride, startup tedesca che ha recentemente raccolto capitali per 50 milioni di dollari. Byd, l’azienda cinese nota per essere tra i principali produttori al mondo di batterie, ha presentato i nuovi modelli di auto elettriche. Parliamo di tecnologia dei veicoli elettrici con Gianmaria Adamo, country manager BYD. Con Innocenzo Genna parliamo di Meta e della neonata versione a pagamento per gli utenti europei. E come sempre, Digital News, le notizie di tecnologia e innovazione più importanti della settimana.

ICMDA Recordings
Webinar #160 Dr André Van Mol - Addressing Transgenderism

ICMDA Recordings

Play Episode Listen Later Aug 4, 2023 57:44


Recorded on 04 August 2023 for ICMDA Webinars. Dr Mark Pickering chairs a webinar with Dr André Van Mol. Gender dysphoria is a diagnosis, but transgenderism is an ideology. Gender transition procedures (GTPs) imperil already at-risk gender dysphoric youth with experimental and unproven hormonal and surgical gender procedures, which medicalize prematurely and permanently. Transition procedures are not proven effective, not proven safe, do not reduce suicides, and are not the standard of care for gender dysphoria. Comprehensive literature reviews are driving an international pushback against GTPs in favor of intensive psychological evaluation and support for both the patient and their family, and the lawsuits over the harms of GTPs have begun. GTPs are not evidence-based care for gender dysphoric youth. André Van Mol, MD is a board-certified family physician in full-time practice in California. He co-chairs both the American College of Pediatricians' Council on Adolescent Sexuality and the Christian Medical & Dental Associations Sexual & Gender Identity Task Force. Dr. Van Mol writes and speaks for the US CMDA on issues of bioethical and Christian concern. He works with Alliance Defending Freedom in a coalition of professionals advising on policy matters addressing sexual orientation and gender identity. He advises legislators, government agencies, and legal and advocacy organizations internationally on these issues. Dr. Van Mol serves on the boards of Bethel Church of Redding and Moral Revolution.

Why Struggle? Podcast w Barbara J. Faison

Week 25 - My M.A.P  All the links are at ⁠⁠https://linktr.ee/barbarafaison⁠⁠⁠ ⁠CHECK OUT THE NATIONAL MEDITATION PLAYLIST ON YOUTUBE⁠ Dear God, what positive, creative, constructive, thought, word, attitude, or action is the next step to move us forward in life? This is the prayer my husband, TD, and I say before he goes to work at oh dark 30. About 5:15 AM most days. It is adapted from one by Catherine Ponder. We each also say a personal prayer where we include appreciation for each other. How do you start your day? I love starting my day with prayer and gratitude. I've heard that without a map, or direction, you could end up anywhere. My M.A.P. is Meditations. Affirmations. Prayers. These are some of my practices. You may have heard me say that meditation is not a goal, it is a practice. Practice means to perform an activity, repeatedly or regularly to improve, or maintain one's proficiency. Train your brain. Be kind to your mind. Pay attention to what you're thinking about and how you feel. We are all creators. I create meditations, videos, and content. Every moment you are creating with your thoughts, feelings, emotions, and your state of being. Setting clear intentions can help with creating and manifesting what you desire in your life. Yes, you, too, are a creator. What we focus on expands and grows like seeds planted in a garden. If we focus on something we don't have, that continues to expand and grow. It may end up being a weed. If we have gratitude for what we do have, that expands and grows. Did you know you can train your brain? It's one reason I love affirmations. Affirmations are positive, portable, present tense and powerful phrases to direct your thoughts. Put that beautiful mind to work by bringing in your desires using focus, intention and attention. Back in the 90s I started using affirmations regularly and eventually it became a daily practice for me. Thoughts are real and they also have energy. I learned to train my mind to use what I call GTPs, go to phrases. GTPs are affirmations that I have trained my mind to focus on. So when I find myself traveling down, anxious avenue, depressed drive, or crazy circle, I redirect my thoughts to one of my GTPs like, in this moment all is well. One of the ways I manage my energy and thoughts during the day is using my phone to remind me. I called these affirmation alarms. Throughout my day I have my phone set to alarm with go to phrases. When the alarm goes off, I take note of where my thoughts are. What am I thinking at that moment? This is a pattern interrupt. I say the affirmation out loud three times. This practice allows me to have awareness so I can redirect my thinking. I can set an intention to focus on something else that I desire. Now my attention is back on track. At 2:22 PM my alarm pops up with – I am healthy and travel and move with ease. My 4:33 PM alarm pops up with – My services are valuable, and I am well compensated for my work. The 10:30 pm alarm is - I give thanks for this wonderful day. Thoughts are alive. What are you thinking about? Everyone is creating every day with what they are thinking and where they are focusing their attention. You can use a simple affirmation such as I am safe, all is well or I am willing to trust myself. Life is all about practice. Whatever we practice we strengthen, grow, and expand in our lives. My M.A.P., meditations, affirmations and prayers are the foundation of my day. Here's a longer one I say that sets the tone for my day. I've been saying this one for over five years. Keep reading on my website. All the links are at ⁠⁠⁠https://linktr.ee/barbarafaison⁠⁠⁠⁠ --- Send in a voice message: https://podcasters.spotify.com/pod/show/barbara-faison/message Support this podcast: https://podcasters.spotify.com/pod/show/barbara-faison/support

Red Mist Podcast
S2 Ep23: Ferrari Sales are Up!

Red Mist Podcast

Play Episode Listen Later Jun 14, 2023 81:09


Welcome to our motorsports podcast where we discuss F1, Indycar, IMSA, NASCAR, our own racing adventures, and some other adventures!The centenarian running of the 24h of Le Mans took place this past weekend and it put on a show! This was the first year of all of the new hypercars & GTPs on track together and it was a great battle! Through some cautions and strategy, I believe it was 5 manufacturers were able to hold the lead at some point during the race but it was Ferrari that came out on top! Corvette was able to cap off the GTE era at Le Mans with a win and Euro Interpol took LMP2.Upcoming this weekend, Indycar returns to the freshly repaved Road America and F1 is in Canada! Can Lawerence Stroll's Aston Martin team get a victory on home turf?!?

Red Mist Podcast
S2 Ep20: Darlington, Indy GP, Laguna, & NHKA

Red Mist Podcast

Play Episode Listen Later May 17, 2023 87:17


Welcome to our motorsports podcast where we discuss F1, Indycar, IMSA, NASCAR, our own racing adventures, and some other adventures!NASCAR returns to Darlington for their throwback weekend and it sure was a classic as they were bumping and banging. Indycar continues it's month of May at the Indianapolis Motor Speedway with a round using the road course this past weekend. IMSA wraps up it's west coast visit with 38 cars, including the brand new GTPs, at Weathertech Raceway Laguna Seca in Monterey, CA. We wrap up this week's episode discussing NHKA's 1st round and how Red Mist Racing got on with our karting adventures!

Momento Agrícola
2022.11.19- Bloco 2 A Pecuária de Corte Sustentavel

Momento Agrícola

Play Episode Listen Later Nov 19, 2022 12:00


A Luiza Bruscato, coordenadora da Mesa Grupo de Trabalho da Pecuária Sustentável do Brasil, explica como o GTPS atua para melhorar a sustentabilidade e a imagem da nossa pecuária de corte no Brasil e no mundo.

Radar Agro
Caio Penido transformou o Fala Carlão em Domingão do Carlão | DC

Radar Agro

Play Episode Listen Later Oct 9, 2022 29:00


Domingão do Carlão conversa com Caio Penido, o produtor rural, cineasta e Ex-Presidente do GTPS, Mesa Brasileira da Pecuária Sustentável e atual Presidente do IMAC, o Instituto Mato-Grossense da Carne. Ele transformou o que era para ser um Fala Carlão num extraordinário Domingão do Carlão. Acompanhe aí! Fala Caio!

Nothing But The Truth
Brian Breach Online Sensation, as Homeless #ViralGuy, tells us how to Crushit on Social Media!

Nothing But The Truth

Play Episode Listen Later Aug 17, 2022 27:37


Brian Breach viral sensation, as homeless viral guy giving away $100

Radar Agro
O novo vice do GTPS | Fala Carlão

Radar Agro

Play Episode Listen Later Aug 17, 2022 14:38


Fala Carlão conversa com o novo Vice Presidente do GTPS, a Mesa Brasileira da Pecuária Sustentável, que congrega os grandes elos da cadeia produtiva brasileira, do pasto ao prato. Nesta prosa, ele fala um pouco de sua história e dos seus novos desafios. Fala Netto!

WiSH Podcasts
GTPS and Groin Pain with Dr Joanne Kemp

WiSH Podcasts

Play Episode Listen Later Jul 11, 2022 35:35


In this episode we discuss greater trochanteric pain syndrome (GTPS) as well as groin related pathologies. Joanne tells us about the pathophysiology of GTPS, the typical presentation, the most up-to-date assessment strategies, as well as the current best-practice management of the condition. We also asked about groin related pain, current terminology, assessment criteria and management, and a lot more! https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4852446/

AAOMPT Podcast
Ep. 96 Fearon Interview

AAOMPT Podcast

Play Episode Listen Later Jun 23, 2022 28:24


Dr. Angie Fearon (e-mail,  Google Scholar, Twitter) of the University of Canberra is interviewed by Dhinu Jayaseelan regarding a publication from BMC Musculoskeletal Disorders titled, “The natural history of greater trochanteric pain syndrome: an 11-year follow-up study.” This episode contains information that will be interesting for practitioners who want to improve their understanding of an understudied but ever-present diagnosis associated with clinical orthopaedics. Additionally, to find the LEAP study mentioned during the interview about exercise and education for GTPS use this link (Mellor et al 2018). Dr. Fearon also recommends checking out Ganderton et al 2017 (Title: Gluteal Loading Versus Sham Exercises to Improve Pain and Dysfunction in Postmenopausal Women with Greater Trochanteric Pain Syndrome: A Randomized Controlled Trial).Find out more about the American Academy of Orthopaedic Manual Physical Therapists at the following links:Academy website: www.aaompt.orgTwitter: @AAOMPTFacebook: https://www.facebook.com/aaompt/Instagram: https://www.instagram.com/officialaaompt/?hl=enPodcast e-mail: aaomptpodcast@gmail.comPodcast website: https://aaomptpodcast.simplecast.fm

BJSM
Menopause therapy and greater trochanteric pain syndrome with Racheal Cowan and Jill Cook. EP #505

BJSM

Play Episode Listen Later May 6, 2022 25:27


In this podcast we are joined by Rachael Cowan and Professor Jill Cook. They provide an update on the evidence for management of greater trochanteric pain syndrome (GTPS) and discuss the results of a recent clinical trial evaluating the effect of menopausal hormone therapy, exercise, and education on tendon pain and function in post-menopausal women with GTPS. On the eve of retirement, Jill provides an opinion on what the next big breakthroughs in tendinopathy research should be! Cowan et al. 2021 https://journals.sagepub.com/doi/full/10.1177/03635465211061142 Ganderton et al. 2018 https://www.liebertpub.com/doi/abs/10.1089/jwh.2017.6729 Mellor et al. 2018 https://www.bmj.com/content/361/bmj.k1662.long

Radar Agro
Ela dá as cartas na mesa | Fala Carlão

Radar Agro

Play Episode Listen Later Apr 16, 2022 18:01


Fala Carlão conversa com Luiza Bruscato, a Engenheira Ambiental que comanda a gerência executiva do GTPS, que é a Mesa Brasileira da Pecuária Sustentável. Conheça um pouco mais do seu trabalho e de sua visão da sustentabilidade.

Reptile Talk
Episode EIGHTY - Matt Baronak (SaSobek Reptiles)

Reptile Talk

Play Episode Listen Later Jan 30, 2022 69:04


 If you're not familiar with the name Sasobek Reptiles - go Google it - then come back for the episode!   A force in Leopard Geckos, former zoo keeper and aquarist - Matt has a lot of notches on his belt. Currently he's got quite the collection of not just leopard geckos, but also GTPs, Tree Monitors and more!  Don't miss this episode! Reptile Talk is Sponsored By:BLACK BOX CAGESWebsitehttps://bit.ly/BLKBoxIGhttp://bit.ly/BLKBOXIGFacebookhttp://bit.ly/BLKBOXFBFIND MATT ON FB & IGhttps://www.facebook.com/sasobekreptileshttps://www.instagram.com/sasobek/?hl=en FOLLOW USARKUSARK.orgFIND US ON IGhttps://bit.ly/ReptileTalkIGhttps://bit.ly/creepingitrealIGhttps://bit.ly/BMRIGFIND US ON FBhttp://bit.ly/ReptiletalkFBhttps://bit.ly/BMRFBAudio Only Versionhttps://bit.ly/Listen2ReptileTalk

Radar Agro
Sustentabilidade é o tema deste papo com o Presidente do GTPS | Fala Carlão

Radar Agro

Play Episode Listen Later Nov 25, 2021 17:14


Fala Carlão conversa com Sérgio Schuler, Vice-Presidente de Ruminantes da Tortuga, marca DSM e que acaba de assumir a Presidência do GTPS, Grupo de Trabalho da Pecuária Sustentável. Conheça aqui os enormes desafios à frente deste executivo. Fala aí Sergio.

My First Kicks
Lex One GTPS

My First Kicks

Play Episode Listen Later Apr 30, 2021 73:51


This week, I welcome the extremely dope Lex One. We talk about growing up in Queens, NY. Being Polo head and doing whatever it took to get his Polo on. We also talk about how he became a platinum recording artist and how sneakers paid for his music career! Tune-In and don't forget to leave a review & subscribe! Follow Lex: Socials: @LexOneGTPS No Hype: https://www.youtube.com/channel/UCnmrdhE6Rw23pgtLzTKt0hw  Follow the pod: Got a my kicks story? email us at myfirstkickspod@gmail.com our IG is @myfirstkickspod Linktree: https://linktr.ee/myfirstkicks

Ghost Travelers Podcast
Episode 5 First paranormal experience in Gettysburg, pa

Ghost Travelers Podcast

Play Episode Listen Later Feb 18, 2021 4:47


In this episode of GTP i thought i would tell you guys my very first ghost sighting that got me into the paranormal & that also started our group Ghost Traveler's, i hope you guys like this episode because this is one of my favorite stores to tell when someone ask me how i got into the paranormal is is why. Things to do in Gettysburg links: Gettysburg Website: https://destinationgettysburg.com Farnsworth House Inn Website: http://www.farnsworthhouseinn.com Ghost Travelers Podcast Instagram page: @pj_gtp Ghost Traveler's Youtube Channel: https://www.youtube.com/user/pjkilgorez Music for GTPs episode Spencer Joyce music: https://www.facebook.com/spencerjoycemusicx

AAOMPT Podcast
Research - French Interview

AAOMPT Podcast

Play Episode Listen Later Sep 3, 2020 39:36


Dr. Helen French of the Royal College of Surgeons in Ireland is interviewed by Dhinu Jayaseelan regarding a publication from Musculoskeletal Science and Practice titled, “An international survey of current physiotherapy practice in diagnosis and knowledge translation of greater trochanteric pain syndrome (GTPS).”

AAOMPT Podcast
Ep. 49 French Interview

AAOMPT Podcast

Play Episode Listen Later Sep 3, 2020 39:36


Dr. Helen French (e-mail, ResearchGate, Twitter) of the Royal College of Surgeons in Ireland is interviewed by Dhinu Jayaseelan regarding a publication from Musculoskeletal Science and Practice titled, “An international survey of current physiotherapy practice in diagnosis and knowledge translation of greater trochanteric pain syndrome (GTPS).” This episode contains information that will be interesting for practitioners who want to learn more about how physical therapists internationally are managing GTPS.Find out more about the American Academy of Orthopaedic Manual Physical Therapists at the following links:Academy website: www.aaompt.orgTwitter: @AAOMPTFacebook: https://www.facebook.com/aaompt/Instagram: https://www.instagram.com/officialaaompt/?hl=enPodcast e-mail: aaomptpodcast@gmail.comPodcast website: https://aaomptpodcast.simplecast.fm

Chat with Betty
EP 5: Independent Mindset with Brian Breach

Chat with Betty

Play Episode Listen Later Jun 24, 2020 31:46


Join me today on Episode 5 of Chat With Betty! Thrilled to chat today with Brian about The Independent Mindset! What is this? How can we get there? On this episode we will also get to know Brian a bit more, and I mean, get inspired from him and his truly incredible back story... Brian Breach is a creator, entertainer, entrepreneur, recent Ted Talk speaker and a social media influencer. His journey started off in the world of music where he spent a decade performing and opening for some of the biggest names in hip hop, as well as having his song air nationally as the intro to a TV show for 3 years. In the process of making music he was able to co-found GTPS independent label and a highly successful hip hop blog getthatpaperson.com. Brian then also created Sikey Clothing and co-founded GTPS printing. Eventually, he stepped away from music and made a name for himself in the world of viral videos and sketch comedy. Brian plans to continue making his mark on earth while empowering others through motivational and positive content. He has also recently started working to launch his own TV show called America's Gone Viral with his partner in the endeavor Christine Curran the current host of Dining Divas. It will be the first ever viral competition show for content creators and it will be launched in 2020. Please find him at @brianbreach on instagram! Cant get enough? Follow us @chatwithbetty on instagram and behind the scenes of our producer @bettygulko --- This episode is sponsored by · Anchor: The easiest way to make a podcast. https://anchor.fm/app Support this podcast: https://anchor.fm/betty-gulko/support

Agrocast
Papo Agro 58 - Entendendo a jornada da pecuária sustentável

Agrocast

Play Episode Listen Later May 17, 2020 36:39


A crescente preocupação com o meio ambiente, o aquecimento global e até os novos hábitos alimentares fez com que a cadeia produtiva da bovinocultura começasse a ser questionada. Dos dejetos gerados na produção, até o bem-estar animal, tudo tem sido alvo de discussão. No entanto, seguimos com a produção animal como importante atividade para o país e o Brasil como grande mercado consumidor e exportador destes produtos. Diante dessa perspectiva, o que o mercado tem feito para se adaptar a essas demandas? É possível pensar em uma produção com critérios mais rigorosos para a sustentabilidade? Pecuária sustentável é tema do episódio dessa semana. Para responder a estes questionamentos e debater sobre esse assunto, o Papo Agro convidou a Engenheira Agrônoma, consultora do GTPS, Stephanie Ferreira. Ficou curioso pra saber o que mais rolou nesse papo? Vai lá no seu agregador de podcast favorito ou no site do Papo Agro e confira o episódio completo.

Agrocast
PA58 - Entendendo a jornada da Pecuária Sustentável

Agrocast

Play Episode Listen Later May 13, 2020 36:40


A crescente preocupação com o meio ambiente, o aquecimento global e até os novos hábitos alimentares fez com que a cadeia produtiva da bovinocultura começasse a ser questionada. Dos dejetos gerados na produção, até o bem-estar animal, tudo tem sido alvo de discussão. No entanto, seguimos com a produção animal como importante atividade para o país e o Brasil como grande mercado consumidor e exportador destes produtos. Diante dessa perspectiva, o que o mercado tem feito para se adaptar a essas demandas? É possível pensar em uma produção com critérios mais rigorosos para a sustentabilidade?  Pecuária sustentável é tema do episódio dessa semana. Para responder a estes questionamentos e debater sobre esse assunto, o Papo Agro convidou a Engenheira Agrônoma, consultora do GTPS, Stephanie Ferreira.  Ficou curioso pra saber o que mais rolou nesse papo? Vai lá no seu agregador de podcast favorito ou no site do Papo Agro e confira o episódio completo. Rede AGROCAST: @redeagrocast @agroresenha @bugbitespodcast @cpvpodcast @esalqast @noticias_do_front @papoagropodcast @benditoagro @rumencast See omnystudio.com/listener for privacy information.

Papo Agro Podcast: Vamos falar sobre o Agro?
PA58 - Entendendo a jornada da Pecuária Sustentável

Papo Agro Podcast: Vamos falar sobre o Agro?

Play Episode Listen Later May 13, 2020 36:40


A crescente preocupação com o meio ambiente, o aquecimento global e até os novos hábitos alimentares fez com que a cadeia produtiva da bovinocultura começasse a ser questionada. Dos dejetos gerados na produção, até o bem-estar animal, tudo tem sido alvo de discussão. No entanto, seguimos com a produção animal como importante atividade para o país e o Brasil como grande mercado consumidor e exportador destes produtos. Diante dessa perspectiva, o que o mercado tem feito para se adaptar a essas demandas? É possível pensar em uma produção com critérios mais rigorosos para a sustentabilidade?  Pecuária sustentável é tema do episódio dessa semana. Para responder a estes questionamentos e debater sobre esse assunto, o Papo Agro convidou a Engenheira Agrônoma, consultora do GTPS, Stephanie Ferreira.  Ficou curioso pra saber o que mais rolou nesse papo? Vai lá no seu agregador de podcast favorito ou no site do Papo Agro e confira o episódio completo. Rede AGROCAST: @redeagrocast @agroresenha @bugbitespodcast @cpvpodcast @esalqast @noticias_do_front @papoagropodcast @benditoagro @rumencast See omnystudio.com/listener for privacy information.

Public News Service
PNS Daily Newscast - April 24, 2020,

Public News Service

Play Episode Listen Later Apr 24, 2020 6:00


Scientists at University of Oxford conduct human trials on a COVID-19 vaccine; and fewer young people are being sent to juvenile detention in the U.S. during the pandemic.

Greenville Seminary & Mt. Olive
#223 - Recent Grad Interview - Florian Weicken

Greenville Seminary & Mt. Olive

Play Episode Listen Later Feb 12, 2020 29:00


In this edition of the podcast we talk with recent GTPS graduate Florian Weicken, presently serving in a church planting work of the Evangelical Presbyterian Church of England and Wales -EPCEW- in Z-rich, Switzerland. For more information about the EPCEW plant in Z-rich, please visit zuerichpres.ch. To support financially the work, please visit https---give.globalservicenetwork.org-51219002.

Confessing Our Hope: The Podcast of Greenville Presbyterian Theological Seminary

In this edition of the podcast we talk with recent GTPS graduate Florian Weicken, presently serving in a church planting work of the Evangelical Presbyterian Church of England and Wales (EPCEW) in Zürich, Switzerland. For more information about the EPCEW plant in Zürich, please visit zuerichpres.ch. To support financially the work, please visit https://give.globalservicenetwork.org/51219002.

Passion & Persistence with host Elaina Mitchell
How "Going Viral" really works | Brian Breach & Elaina Mitchell | Passion & Persistence S2E3

Passion & Persistence with host Elaina Mitchell

Play Episode Listen Later Jan 20, 2020 35:01


Join us as we chat with Brian Breach "That Viral Guy", about the importance of using your platform for good, and how "going viral" really works.Meet Brian Breach: Brian Breach is a creator, entertainer, entrepreneur, recent Tedx Talk speaker and a social media influencer. His journey started off in the world of music where he spent a decade performing and opening for some of the biggest names in hip hop as well as having his song air nationally as the intro to a TV show for 3 years. In the process of making music he was able to co found GTPS independent label & a highly successful hip hop blog getthatpaperson.com. As time passed he also created Sikey Clothing and co founded GTPS printing. Eventually stepping away from music he has made a name for himself in the world of viral videos/sketch comedy with one of his first social experiments going internationally viral, having the story being featured on FOX, MSNBC, CNN, ABC, NBC, Inside Edition, Yahoo and many other Media outlets. Brian is currently verified on Instagram with over 85,000 followers and a growing fan base. Follow Brian: @BrianBreachFollow us: @passion.and.persistenceVisit us online at passionandpersistencepodcast.comLearn more about host Elaina Mitchell by visiting www.emitchelldesigns.com or @Elaina.mitchell on Instagram.

The Check-up
Hot Topics GP Update 2 - Greater Trochanteric Pain Syndrome (GTPS)

The Check-up

Play Episode Listen Later Oct 24, 2019 12:15


Hot Topics GP Update 2 - Greater Trochanteric Pain Syndrome (GTPS) by Podcast by Medcast

Automating Quality
Innovation to Retrovation: Maintaining GTP in a GxP World, with Jay Turner

Automating Quality

Play Episode Listen Later Jun 25, 2019 32:39


Welcome to the 23rd episode of Automating Quality. Mandy Gervasio is your host, and she’s on a mission to bring you hot industry topics with her fellow life sciences partners and friends!   Today, Mandy is joined by her co-host, Philippe Gaudreau, and return guest from episodes two and ten, Jay Turner! Jay is the CEO and founder of EduJay LLC., and he is a self-proclaimed learning and development ninja! He has devoted his career to improving people, processes, and programs to produce business results. This includes everything from implementing learning management systems, to designing staff development and leadership development programs, to providing executive coaching.   In this episode, Mandy and Jay are discussing innovation to retrovation — an interesting concept about maintaining GTP (AKA Good Training Practices) in a GxP world. Jay gives his expert feedback on the concepts of GTP, explains what he means by “retrovate” and how it applies to training, gives his top three favorite GTPs and how they can be leveraged in the quality space, and also explains how we can effectively innovate in training when we know it’s needed. The feedback that Jay provides in this episode will be incredibly beneficial for listeners who are considering enhancements to their current training and professional development programs. So tune in to get all of his valuable insight on today’s topic!   Key Takeaways: [:32] About today’s episode with return guest, Jay Turner! [2:37] Mandy welcomes Jay back to the show! [2:49] What does Jay mean by “retrovate” and how does it apply to training? [7:12] Jay lists his top two out of three GTPs and how they can be leveraged in the quality space. [14:20] Jay speaks about moving beyond required training and empowering staff members to become a part of the learning function in the organization. [17:50] Jay explains his third favorite GTP. [21:26] How can we effectively innovate in training when we know it’s needed? [28:07] Mandy thanks Jay for joining her this episode! [29:05] Jay gives a quick background about himself and his organization, EduJay. [31:24] Mandy wraps us this week’s episode by thanking listeners for tuning and giving a quick summary of today’s episode!   Mentioned in this Episode: EduJay LLC Jay Turner’s Twitter Jay Turner’s Facebook Jay Turner’s Linkedin Jay@EduJay.com Automating Quality Ep. 10: “Social and Collaborative Learning with Jay Turner” Automating Quality Ep. 02: “Optimizing Compliance When Implementing an Automated Training Solution with Jay Turner” Mandy.Podcast@Solabs.com Retrovate or Retrovation GTP Google VR Oculus Rift Design Thinking

Morelia pythons radio
GTP's with Evan Browder from Carpet Cartel

Morelia pythons radio

Play Episode Listen Later Apr 16, 2019 145:00


In this episode, we are joined by our good friend, Evan Browder from Carpet Cartel. We will be talking about his experiences working with gtps and we will hot on some of his future projects. Morelia Python Radio Carpet Cartel    

cartel carpet morelia browder gtps green tree pythons chondros
Morelia pythons radio
Vin Russo from Cutting Edge Herpetoculture joins us.

Morelia pythons radio

Play Episode Listen Later Jan 22, 2019 148:00


In this episode, we are joined by Vin Russo from Cutting Edge Herpetological. We will be talking about a variety of topics from boas to gtp's. Vin has a ton of experience with many different species of reptiles and we will be talking about them all. Cutting edge herpetological

Agrocast
ARP#060 - Pecuária sustentável

Agrocast

Play Episode Listen Later Oct 28, 2018 42:08


Neste episódio, Paulo conversa com a Beatriz Domeniconi, coordenadora executiva do GTPS, o Grupo de Trabalho da Pecuária Sustentável. Ainda que o termo sustentabilidade seja interpretado de maneira equivocada e a pecuária seja vista como a “vilã” do meio ambiente, a produção de carne bovina é importantíssima para a economia do Brasil e precisa ser valorizada pela sociedade. =============== LINKS CITADOS NO EPISÓDIO Site do GTPS: http://gtps.org.br LinkedIn GTPS: https://www.linkedin.com/company/gtps---brazilian-roundtable-on-sustainable-livestock-brsl-/ Instagram: https://www.instagram.com/gtps_pecuaria/ YouTube: https://www.youtube.com/channel/UC476WbFBPO8dfQ9tJl1l4RQ?view_as=subscriber Facebook: https://www.facebook.com/gtpsbrasil Twitter: https://twitter.com/gtps_brasil Sustentabilidade: da evolução dos conceitos à implementação como estratégia nas organizações: http://www.scielo.br/pdf/prod/2011nahead/aop_0007_0245.pdf Os “Limites do Crescimento” 40 anos depois: http://www.scielo.br/pdf/rbcsoc/v28n81/18.pdf Livestock's long shadow: http://www.fao.org/docrep/010/a0701e/a0701e00.HTM CONTATOS DA BEATRIZ E-mail: beatriz@gtps.org.br LinkedIn: https://www.linkedin.com/in/beatriz-domeniconi-145a5426/ INTERAJA COM O AGRO RESENHA Twitter: twitter.com/AgroResenha Instagram: www.instagram.com/agroresenha Facebook: www.facebook.com/agroresenha WhatsApp: http://bit.ly/agronozapzap / +55 (65) 99900-8973 E-MAIL Se você tem alguma sugestão de pauta, reclamação ou dúvida envie um e-mail para agroresenha@gmail.com PADRINHOS E MADRINHAS DO AGRO RESENHA Paulo Henrique Sá Fortes Mariely Biff Fabio Makoto Okuno Michael Ortigara Goulart Luciano Mendes César Kobayakawa Cleomar Amaral Michely Santana Lucas Fuchs Cesar Augusto da Silva Bessa Paulo Massaharu Ozaki Maria Luisa de Moraes Ozaki Fernando Borges Luiz Fernando Sacchett Dias Geide Antonio Figueiredo Junior Rondiny Carneiro Jaime Sanchez da Cruz Rios Marcos Mamoru Fugio Otto Ozaki André Tavares de Vasconcelos Carla Papai Diego Henrique Uroda Albert Kenji Hirose Nayara Nunes Rodrigues Valter Galan FICHA TÉCNICA Roteiro e produção: Paulo Ozaki Edição: Senhor A - https://editorsenhor-a.com.br Convidada: Beatriz Domeniconi See omnystudio.com/listener for privacy information.

Agro Resenha Podcast
ARP#060 - Pecuária sustentável

Agro Resenha Podcast

Play Episode Listen Later Oct 28, 2018 42:07


Neste episódio, Paulo conversa com a Beatriz Domeniconi, coordenadora executiva do GTPS, o Grupo de Trabalho da Pecuária Sustentável. Ainda que o termo sustentabilidade seja interpretado de maneira equivocada e a pecuária seja vista como a “vilã” do meio ambiente, a produção de carne bovina é importantíssima para a economia do Brasil e precisa ser valorizada pela sociedade. =============== LINKS CITADOS NO EPISÓDIO Site do GTPS: http://gtps.org.br LinkedIn GTPS: https://www.linkedin.com/company/gtps---brazilian-roundtable-on-sustainable-livestock-brsl-/ Instagram: https://www.instagram.com/gtps_pecuaria/ YouTube: https://www.youtube.com/channel/UC476WbFBPO8dfQ9tJl1l4RQ?view_as=subscriber Facebook: https://www.facebook.com/gtpsbrasil Twitter: https://twitter.com/gtps_brasil Sustentabilidade: da evolução dos conceitos à implementação como estratégia nas organizações: http://www.scielo.br/pdf/prod/2011nahead/aop_0007_0245.pdf Os “Limites do Crescimento” 40 anos depois: http://www.scielo.br/pdf/rbcsoc/v28n81/18.pdf Livestock’s long shadow: http://www.fao.org/docrep/010/a0701e/a0701e00.HTM CONTATOS DA BEATRIZ E-mail: beatriz@gtps.org.br LinkedIn: https://www.linkedin.com/in/beatriz-domeniconi-145a5426/ INTERAJA COM O AGRO RESENHA Twitter: twitter.com/AgroResenha Instagram: www.instagram.com/agroresenha Facebook: www.facebook.com/agroresenha WhatsApp: http://bit.ly/agronozapzap / +55 (65) 99900-8973 E-MAIL Se você tem alguma sugestão de pauta, reclamação ou dúvida envie um e-mail para agroresenha@gmail.com PADRINHOS E MADRINHAS DO AGRO RESENHA Paulo Henrique Sá Fortes Mariely Biff Fabio Makoto Okuno Michael Ortigara Goulart Luciano Mendes César Kobayakawa Cleomar Amaral Michely Santana Lucas Fuchs Cesar Augusto da Silva Bessa Paulo Massaharu Ozaki Maria Luisa de Moraes Ozaki Fernando Borges Luiz Fernando Sacchett Dias Geide Antonio Figueiredo Junior Rondiny Carneiro Jaime Sanchez da Cruz Rios Marcos Mamoru Fugio Otto Ozaki André Tavares de Vasconcelos Carla Papai Diego Henrique Uroda Albert Kenji Hirose Nayara Nunes Rodrigues Valter Galan FICHA TÉCNICA Roteiro e produção: Paulo Ozaki Edição: Senhor A - https://editorsenhor-a.com.br Convidada: Beatriz Domeniconi

Confessing Our Hope: The Podcast of Greenville Presbyterian Theological Seminary
#177 - Recent Grad Interview - Nathan John Hilton

Confessing Our Hope: The Podcast of Greenville Presbyterian Theological Seminary

Play Episode Listen Later Jun 21, 2018 40:57


In this edition of the podcast we talk with recent GTPS graduate Nathan John Hilton, presently serving in the Evangelical Presbyterian Church of England and Wales (EPCEW) in Gateshead, England, UK.

Confessing Our Hope: The Podcast of Greenville Presbyterian Theological Seminary

In this edition of the podcast we talk with GTPS graduate Bill Hill, currently serving as Pastor of Fellowship Presbyterian Church (PCA) in Newport, TN.

Confessing Our Hope: The Podcast of Greenville Presbyterian Theological Seminary

In this edition of the podcast we talk with GTPS graduate Dr. Vincent Wood, currently serving as Pastor of Providence Presbyterian Church (PCA) in York, PA.

Confessing Our Hope: The Podcast of Greenville Presbyterian Theological Seminary

In this edition of the podcast we talk with GTPS graduate Benjamin Wontrop, currently serving as a pastor in the Evangelical Presbyterian Church of England and Wales, in partnership with Presbyterian Evangelistic Fellowship.

Morelia pythons radio
Green Tree Pythons with Ian Bessell from S & J reptiles!

Morelia pythons radio

Play Episode Listen Later Jan 9, 2018 168:00


We are back from our holiday break and we have an exciting episode to start off the new season of MPR. We are joined by Ian Bessell to talk about S&J Reptiles, GTPs, 2018 Breeding projects and more! We will also be discussing plans for the upcomiong Southeast Carpetfest 2018 which will take place on Febuary 10th in Cape Coral, Fl. Tune in for more details.    

Confessing Our Hope: The Podcast of Greenville Presbyterian Theological Seminary

In this edition of the podcast we talk with GTPS graduate Berti Kona, currently serving as a PCA church planter in Albania.

Confessing Our Hope: The Podcast of Greenville Presbyterian Theological Seminary

In this edition of the podcast we talk with GTPS graduate Mike Cuneo, currently serving as a PCA church planter in Viterbo, Italy.

Confessing Our Hope: The Podcast of Greenville Presbyterian Theological Seminary

In this edition of the podcast we talk with GTPS graduate Ross Fearing, currently serving with Ministry to the Military and International in San Antonio, TX.

Confessing Our Hope: The Podcast of Greenville Presbyterian Theological Seminary

In this edition of the podcast we talk wtih GTPS graduate Nick Batzig, the organizing pastor of New Covenant PCA in Richmond Hill, GA.

Confessing Our Hope: The Podcast of Greenville Presbyterian Theological Seminary

In this edition of the podcast we talk wtih GTPS graduate Lowell Ivey, the organizing pastor of Reformation OPC in Va. Breach, VA.

Medizin - Open Access LMU - Teil 09/22
Exocytosis from permeabilized bovine adrenal chromaffin cells is differently modulated by guanosine 5'-[gamma-thio]triphosphate and guanosine 5'-[beta gamma-imido]triphosphate

Medizin - Open Access LMU - Teil 09/22

Play Episode Listen Later Jan 1, 1992


1. In bovine adrenal chromaffin cells made permeable either to molecules less than or equal to 3 kDa with alphatoxin or to proteins less than or equal to 150 kDa with streptolysin O, the GTP analogues guanosine 5'-[beta gamma-imido]triphosphate (p[NH]ppG) and guanosine 5'-[gamma-thio]triphosphate (GTP[S]) differently modulated Ca(2+)-stimulated exocytosis. 2. In alphatoxin-permeabilized cells, p[NH]ppG up to 20 microM activated Ca(2+)-stimulated exocytosis. Higher concentrations had little or no effect. At a free Ca2+ concentration of 5 microM, 7 microM-p[NH]ppG stimulated exocytosis 6-fold. Increasing the free Ca2+ concentration reduced the effect of p[NH]ppG. Pretreatment of the cells with pertussis toxin prevented the activation of the Ca(2+)-stimulated exocytosis by p[NH]ppG. 3. In streptolysin O-permeabilized cells, p[NH]ppG did not activate, but rather inhibited Ca(2+)-dependent catecholamine release under all conditions studied. In the soluble cytoplasmic material that escaped during permeabilization with streptolysin O, different G-protein alpha-subunits were detected using an appropriate antibody. Around 15% of the cellular alpha-subunits were detected in the supernatant of permeabilized control cells. p[NH]ppG or GTP[S] stimulated the release of alpha-subunits 2-fold, causing a loss of about 30% of the cellular G-protein alpha-subunits under these conditions. Two of the alpha-subunits in the supernatant belonged to the G(o) type, as revealed by an antibody specific for G(o) alpha. 4. GTP[S], when present alone during stimulation with Ca2+, activated exocytosis in a similar manner to p[NH]ppG. Upon prolonged incubation, GTP[S], in contrast to p[NH]ppG, inhibited Ca(2+)-induced exocytosis from cells permeabilized by either of the pore-forming toxins. This effect was resistant to pertussin toxin. 5. The p[NH]ppG-induced activation of Ca(2+)-stimulated release from alphatoxin-permeabilized chromaffin cells may be attributed to one of the heterotrimeric G-proteins lost during permeabilization with streptolysin O. The inhibitory effect of GTP[S] on exocytosis is apparently not mediated by G-protein alpha-subunits, but by another GTP-dependent process still occurring after permeabilization with streptolysin O.

Medizin - Open Access LMU - Teil 09/22
Amylase release from streptolysin O-permeabilized pancreatic acinar cells. Effects of Ca2+, guanosine 5'-[gamma-thio]triphosphate, cyclic AMP, tetanus toxin and botulinum A toxin

Medizin - Open Access LMU - Teil 09/22

Play Episode Listen Later Jan 1, 1992


The molecular requirements for amylase release and the intracellular effects of botulinum A toxin and tetanus toxin on amylase release were investigated using rat pancreatic acinar cells permeabilized with streptolysin O. Micromolar concentrations of free Ca2+ evoked amylase release from these cells. Maximal release was observed in the presence of 30 microM free Ca2+. Ca(2+)-stimulated, but not basal, amylase release was enhanced by guanosine 5'-[gamma-thio]triphosphate (GTP[S]) (3-4 fold) or cyclic AMP (1.5-2 fold). Neither the two-chain forms of botulinum A toxin and tetanus toxin, under reducing conditions, nor the light chains of tetanus toxin, inhibited amylase release triggered by Ca2+, or combinations of Ca2+ + GTP[S] or Ca2+ + cAMP. The lack of inhibition was not due to inactivation of botulinum A toxin or tetanus toxin by pancreatic acinar cell proteolytic enzymes, as toxins previously incubated with permeabilized pancreatic acinar cells inhibited Ca(2+)-stimulated [3H]noradrenaline release from streptolysin O-permeabilized adrenal chromaffin cells. These data imply that clostridial neurotoxins inhibit a Ca(2+)-dependent mechanism which promotes exocytosis in neural and endocrine cells, but not in exocrine cells.