Podcasts about trimer

durée : 00:58:27 - Le Cours de l'histoire - par : Xavier Mauduit - Des villes aux campagnes, l'esclavage est partout dans le monde médiéval. Distincts des serfs, les esclaves sont toujours des étrangers, considérés comme des êtres-marchandises aux frontières de l'humanité. - invités : Sandrine Victor Professeure d'histoire médiévale à l'Université d'Albi, chercheuse au laboratoire FRAMESPA, spécialiste de l'économie et de la société du chantier médiéval

Bonjour Menestres, Mesdames et Messieurs et bienvenue dans On n'a rien préparé ! C'est un bonheur de vous accueillir pour cinquième saison d'impros, d'invité‧e‧s, de joie, de blagues, de bêtises en tout genre et plus si affinité ! Dans cet épisode : 06:00 : on vous partage une trouvaille du web par Iseult : Hacker-Typer,11:50 : on chante une année dans la vie d'un grizzli,19:00 : on plonge dans le calvaire de Noisette et Fougère en maraboutant,24:00 : on vous partage nos coups de cœur ! La trouvaille du web d'Iseult est Hacker-Typer (https://hackertyper.com/ ). Le coup de cœur d'Iseult est pour « Je suis tienne irrévocablement », un recueil de lettres de Renée Vivien à Nathalie Barnet (https://www.editions-bartillat.fr/fiche-livre.php?Clef=571 ). Le coup de cœur de Manu est pour « Transparent », une série créée par Joey Soloway diffusée sur une plateforme de streaming bien connue appartenant à une personne qui fait voler des vaisseaux spatiaux entre autres absurdités (https://fr.wikipedia.org/wiki/Transparent_(s%C3%A9rie_t%C3%A9l%C3%A9vis%C3%A9e) . Le coup de cœur d'Hicham est pour « Against the Storm », un jeu vidéo de Eremite Games (https://www.senscritique.com/jeuvideo/Against_the_Storm/45194723 ). Prenez bien soin de vous et à la semaine prochaine !

Aujourd'hui dans Véronique et les Fantastiques, avec Louis Morissette, on parle des personnalités qui osent exposer leur vie au public…on est complètement Mardi Sexy avec Geneviève qui nous dévoile comment se passe sa sexualidade à 24 semaines de grossesse et Mona de Grenoble nous partage de belles lettres d'amour ! Bonne écoute!

November 3, 2023 ~ Mike Falahee, President of Marygrove Awning, Margaret Trimer, Vice President of Strategic Partnerships for Delta Dental and Rodney Cole, President of the DTE Energy Foundation and DTE Corporate Citizenship join Guy and Lloyd live from the Parade Company Pancake Breakfast.

Recorded 2023-07-04 19:59:04 Tracklisting: * Mitekiss - Grief in Puddles * London Elektricity - Fast Soul Music (feat. Liane Carroll) [Dogger & Mindstate Remix] * J:Rover, Phoebe Train - Grow - Original Mix * Shy FX - Call Me (feat. Maverick Sabre) * Alix Perez - Moving On (feat. Liam Bailey) [Break Remix] * BCee & DRS - Let Go of Love * Felix Raymon - Lonely Now (feat. Jamal Dilmen) * Emz - Good Good (feat. Kanobie) * Trex, Trimer & Subten - Soul Food (feat. Ella Jones) * Pola & Bryson - Get Serious * DRS - Like Uh (feat. Vangeliez) * Mozey, Shady Novelle & Dynamite MC - Midnight Caller * Nymfo - Do me no good * DRS, HMD & Redeyes - Cinnamon Roses * London Elektricity - Life Is Beautiful * Bcee - Rain (Original Mix) * Workforce - Forever (LSB Remix) * Pat Fulgoni - Beautiful You (The Vanguard Project Remix) * Bert H - Vibechaser * Chase & Status - Weed & Rum (feat. Masicka) * Mollie Collins & Ruth Royall - Remedies (Vibe Chemistry Remix) * LSB - Hotpants Bouncer * Monty, Redeyes, [ K S R ] - Almond - Original Mix * Foreign Concept - Champagne Nihilist * GLXY & Rhi - Sweet Lows * Simplification & Blak - Forever (Villem Remix) * Commix - How You Gonna Feel (feat. Steve Spacek) * Invadhertz, Jamal Dilmen & Felix Raymon - Too Much Download, Distribute, and Donate!

Good evening! Time for the 95th instalment of the Back To The Future podcast. Tracks from across the scene wrapped up in a couple of mixes, a 3From, opener and a closer. Music from Break, Revan, Jubei, QZB and Need For Mirrors plus many more. The 3From is slowed down and soulful with https://soundcloud.com/tyler-daley, really lovely tracks. Let's get into it… Intro The Sauce & Fox - Yagga Yagga (The Sauce) Thematic - In Business (Citate Forms) Break & Liam Bailey - Gunshot Love (Symmetry) Mix1 Total Science - Ploc Monster (Metalheadz)[2010] >> Break & Fats - Natural Way (Symmetry) Q Project - In Plain Sight (C.I.A) The Sauce - On A Mission (The Sauce) Revan & Scepticz - About Time (Sofa Sound) Spinline - Photography (Sofa Sound) L-Side & Fats - High Times (Break remix) (V) Spinline - Keleti (Sofa Sound) The Upbeats - Octo (Vision) Jubei & Skeptical - The Rebellion (Carbon) Skeptical - Push Up (Rubi) M-Zine - DD (Sofa Sound) T>I - Step Back (C.I.A) Skeptical - Thru My Head (Rubi) T>I ft. Madrush MC - Sketchy (C.I.A) 3From - Tyler Daley DRS & Tyler Daley ft. LSB - Too Late (Footnotes) DRS Ft. Dogger & Tyler Daley - Summer (Shogun) DRS & Tyler Daley ft. Calibre - I Remember (Footnotes) Mix2 Charli Brix, Visages & DRS - I Can't Stay (Flexout) >> Sevin - Easy (Fokuz) Visionobi & Mitekiss - Tree Line (Soulvent) Charli Brix & QZB - Thirteen (Flexout) Winslow - Spaced Out (Hospital) Revan, Blue Jay & Scepticz - Night Beats (Sofa Sound) Visionobi, Philth & SOLAH - Weather The Storm (Soulvent) Halogenix - Ivory (Gemini Gemini) Trex, Trimer, Subten ft. Ella Jones - Soul Food (Dazed Music) dBridge ft. Calibre - Ponderosa (Exit) [Classic] Dogger, Mindstate & DRS - TMWFTE (1985) SATL - Drifted (The North Quarter) Need For Mirrors - Mourning Tune (ThirtyOne) En:vy - Beautiful Dreamer (Critical) Last One Doc Scott - Ghost 25 (Metalheadz) #dmix #backtothefuturednb #dmixandevolve #eruptionradio #drumandbass #drumandbass4life #drumandbassclassics #drumandbassrollers #drumandbassonthebike #drumandbassuk #drumandbassmusic #drumandbassfamily #drumandbasslife #dnb #dnbnation #dnb4life #dnblover #dnb4ever #dnb2023 #junglednb #dnbcrew #dnbmix #dnbhead #dnbforlife #dnbuk #dnbrollers #dnbmusic #dnbfamily

403's finest back with another densely packed journey through beauty and delight. Tracklist: Piri + Tommy - Beachin' (Sudley Remix) Dawn Wall – Fears Surreal, Minos - Want U (Surreal Remix) Halogenix – IDGAF Burt Cope – Naked (feat. Lila Lin) Icicle – Nostalgia Reflektor - High Grade Objectiv, Zoro, Lupo, Jappa - Papa's Got Bag Technicolour/Technimatic - The Counting Tune Submorphics – Shadow Red Lovell – Ethanol Changing Faces & In:Most ft. Dale James May – Composure Technimatic & A Little Sound – Confide Hybrid Minds - Never Change (GLXY Remix) Klinical - Lost Vanity Metal Work - Deep As Shadows Kublai/Aaliyah Esprit – Pushin' BCee & SOLAH – Say it Loud Low:r & Aleya Mae – Make the Most Atlantic Connection - Situations (DLR Remix) brk & Speaker Louis – Just Like You Kyrist Feat. Steo – Find a Meaning SOLAH - Love For Me Too Henry & Fullalove – Sub Zero Motiv – Jupiter Pola & Bryson – 24/7 TREX & Trimer & Subten & Ella Jones – Soul Food Klinical – Destined Foreign Beggars & Laville - Reveal (Alix Perez Remix) BCee & Kubiks - Remain Nameless Messiah - Black Hole Riya, Collette Warren & Visages – Sliding Doors Mitekiss/B-ahwe – Droplets Spektiv, Sydney – Moments Enei - Show Me Saxxon – Gelato Tunnl Vision, Heysoul – Tropica Aaron Payne – Orchids Hugh Hardie/Phaction/Javeon – Got U Wrong Geostatic - Don't Let Me Go Hugh Hardie – Coulda Woulda DRS & Javeon & EN:VY – Passenger Trail - Shojo Bookshop Air.K & Cephei – Hold On Motiv & Salem Focus & Surreal - Lazy Saturday Unglued - Way Back When (Halogenix Remix) (feat. Esther Durin) DJ Zinc, Mozey, M.A.R.Y – Get Wiser Sub:liminal, Sydney - Wishes For Your Soul

Listen in on a conversation between two Delta Dental interns as they reflect on their experiences, lessons learned and the impact an internship program can have on both the organization and young professionals.

Recorded 2022-08-23 02:00:32 DnB Radio Tracklisting: * Dan Guidance, Alex Barton - Waiting (Original Mix)* Nelver & Nic ZigZag - Ember Lights* FABRIC8 - Nobodys Love* Vektah - Pocket Rocket* V O E - Running Blind (Original Mix)* Justin Hawkes - Black Bloc (Original Mix)* Phibes - Forever (Original Mix)* Derwitch - Shona (Original Mix)* Bachelors Of Science feat. Pat Fulgoni - Stomp* Trex, Ella Jones, Trimer, Subten - Soul Food feat. Ella Jones (Original Mix)* Minor Forms, Kublai - Do Remember (Original Mix) * Spaow - Delta Warfare * Dr. Apollo - Holy Grail * Chase & Status, Liam Bailey - Blind Faith feat. Liam Bailey (Loadstar Remix) * Total Science, S.P.Y - Try Out feat. Marky* Black Sun Empire - Potemkin (S.P.Y Remix) (48)* Kove - Liberator (Original Mix) (32)* Trex - Dopamine (VIP)* Emba, L.I.T.A. - Holding Back (Original Mix)* DJ Wolf, Mojuba - Leaving Them Damaged - DEMO* Trex, X Bar Theory - Style & Substance (Club mix) feat. Trex (Beatport Exclusive)* Horge X Charles Rebellion - Ghost in the Shell * Unglued - Ichnusa* DC Breaks - Euphorium (Original Mix)* Agressor Bunx - Inner Line* Sub Focus - Off The Ground (Original Mix)* In:Most - Domino Effect* Grafix - Only Now* Delta Heavy - Heartbeat (Extended Mix)* Teddy Killerz - Night Train (Original Mix)* Noisia & IMANU - Shift* Anthropic - Sinistre* Bensley - Hit The Lights (Original Mix)* Demo - Run Them* DJ Hybrid, Trex - Comback (Trex Remix)* Demo - Music Maker (broken)* Vektah - Spectral (broken)* Damian Marley - Welcome to Jamrock (Conrad Subs Bootleg)* Rider Shafique, Trex - Undergrowth (Original Mix)* Prolix & Biometrix - Addicted To The Game* Lateral - BACKHOME* Grafix - CTRL* Sub Focus - Rock It (Wilkinson Remix)* Perplex - Never Stop* Mob Tactics - Wolf Trap * Dose, Teknik, JABZ MC - No Turning Back feat. Teknik feat. Jabz MC (Original Mix) * Skellytn - Particle Beam (ft. MagMag)* Woofax - Kick (Original Mix)* Perplex - Tonight* Tantrum Desire - The Flow (Original Mix)* Mob Tactics - Pure X VIP (Original Mix)* REAPER & Josh Rubin - RUNAWAY* Paperclip - Koef* Krispe - Click Clack* Wilkinson - Frontline (Original Mix)* Lateral - Do It* Pythius - Byss (Original Mix)* Wilkinson - Dirty Love Feat. Talay Riley (Original Mix) * Wilkinson - Direction VIP * Dimension (UK) - Whip Slap (Original Mix) * Dimension - Crowd Reaction VIP * Dimension - UK* Gene Farris, Sub Focus - It's Time (Original Mix)* Levela, Waeys - Gasp (Original Mix)* Perplex - Malfunction* Intercept - Narcos* Wilkinson - Decompression (Original Mix)* Wilkinson - Take You Higher (Original Mix) Tracklisting:* Dan Guidance, Alex Barton - Waiting (Original Mix)* Nelver & Nic ZigZag - Ember Lights* FABRIC8 - Nobodys Love* Vektah - Pocket Rocket* V O E - Running Blind (Original Mix)* Justin Hawkes - Black Bloc (Original Mix)* Phibes - Forever (Original Mix)* Derwitch - Shona (Original Mix)* Bachelors Of Science feat. Pat Fulgoni - Stomp* Trex, Ella Jones, Trimer, Subten - Soul Food feat. Ella Jones (Original Mix)* Minor Forms, Kublai - Do Remember (Original Mix) * Spaow - Delta Warfare * Dr. Apollo - Holy Grail * Chase & Status, Liam Bailey - Blind Faith feat. Liam Bailey (Loadstar Remix) * Total Science, S.P.Y - Try Out feat. Marky* Black Sun Empire - Potemkin (S.P.Y Remix) (48)* Kove - Liberator (Original Mix) (32)* Trex - Dopamine (VIP)* Emba, L.I.T.A. - Holding Back (Original Mix)* DJ Wolf, Mojuba - Leaving Them Damaged - DEMO* Trex, X Bar Theory - Style & Substance (Club mix) feat. Trex (Beatport Exclusive)* Horge X Charles Rebellion - Ghost in the Shell * Unglued - Ichnusa* DC Breaks - Euphorium (Original Mix)* Agressor Bunx - Inner Line* Sub Focus - Off The Ground (Original Mix)* In:Most - Domino Effect* Grafix - Only Now* Delta Heavy - Heartbeat (Extended Mix)* Teddy Killerz - Night Train (Original Mix)* Noisia & IMANU - Shift* Anthropic - Sinistre* Bensley - Hit The Lights (Original Mix)* Demo - Run Them* DJ Hybrid, Trex - Comback (Trex Remix)* Demo - Music Maker (broken)* Vektah - Spectral (broken)* Damian Marley - Welcome to Jamrock (Conrad Subs Bootleg)* Rider Shafique, Trex - Undergrowth (Original Mix)* Prolix & Biometrix - Addicted To The Game* Lateral - BACKHOME* Grafix - CTRL* Sub Focus - Rock It (Wilkinson Remix)* Perplex - Never Stop* Mob Tactics - Wolf Trap * Dose, Teknik, JABZ MC - No Turning Back feat. Teknik feat. Jabz MC (Original Mix) * Skellytn - Particle Beam (ft. MagMag)* Woofax - Kick (Original Mix)* Perplex - Tonight* Tantrum Desire - The Flow (Original Mix)* Mob Tactics - Pure X VIP (Original Mix)* REAPER & Josh Rubin - RUNAWAY* Paperclip - Koef* Krispe - Click Clack* Wilkinson - Frontline (Original Mix)* Lateral - Do It* Pythius - Byss (Original Mix)* Wilkinson - Dirty Love Feat. Talay Riley (Original Mix) * Wilkinson - Direction VIP * Dimension (UK) - Whip Slap (Original Mix) * Dimension - Crowd Reaction VIP * Dimension - UK* Gene Farris, Sub Focus - It's Time (Original Mix)* Levela, Waeys - Gasp (Original Mix)* Perplex - Malfunction* Intercept - Narcos* Wilkinson - Decompression (Original Mix)* Wilkinson - Take You Higher (Original Mix) Download, Distribute, and Donate!

Recorded 2022-08-23 02:00:32 DnB Radio Tracklisting: * Dan Guidance, Alex Barton - Waiting (Original Mix)* Nelver & Nic ZigZag - Ember Lights* FABRIC8 - Nobodys Love* Vektah - Pocket Rocket* V O E - Running Blind (Original Mix)* Justin Hawkes - Black Bloc (Original Mix)* Phibes - Forever (Original Mix)* Derwitch - Shona (Original Mix)* Bachelors Of Science feat. Pat Fulgoni - Stomp* Trex, Ella Jones, Trimer, Subten - Soul Food feat. Ella Jones (Original Mix)* Minor Forms, Kublai - Do Remember (Original Mix) * Spaow - Delta Warfare * Dr. Apollo - Holy Grail * Chase & Status, Liam Bailey - Blind Faith feat. Liam Bailey (Loadstar Remix) * Total Science, S.P.Y - Try Out feat. Marky* Black Sun Empire - Potemkin (S.P.Y Remix) (48)* Kove - Liberator (Original Mix) (32)* Trex - Dopamine (VIP)* Emba, L.I.T.A. - Holding Back (Original Mix)* DJ Wolf, Mojuba - Leaving Them Damaged - DEMO* Trex, X Bar Theory - Style & Substance (Club mix) feat. Trex (Beatport Exclusive)* Horge X Charles Rebellion - Ghost in the Shell * Unglued - Ichnusa* DC Breaks - Euphorium (Original Mix)* Agressor Bunx - Inner Line* Sub Focus - Off The Ground (Original Mix)* In:Most - Domino Effect* Grafix - Only Now* Delta Heavy - Heartbeat (Extended Mix)* Teddy Killerz - Night Train (Original Mix)* Noisia & IMANU - Shift* Anthropic - Sinistre* Bensley - Hit The Lights (Original Mix)* Demo - Run Them* DJ Hybrid, Trex - Comback (Trex Remix)* Demo - Music Maker (broken)* Vektah - Spectral (broken)* Damian Marley - Welcome to Jamrock (Conrad Subs Bootleg)* Rider Shafique, Trex - Undergrowth (Original Mix)* Prolix & Biometrix - Addicted To The Game* Lateral - BACKHOME* Grafix - CTRL* Sub Focus - Rock It (Wilkinson Remix)* Perplex - Never Stop* Mob Tactics - Wolf Trap * Dose, Teknik, JABZ MC - No Turning Back feat. Teknik feat. Jabz MC (Original Mix) * Skellytn - Particle Beam (ft. MagMag)* Woofax - Kick (Original Mix)* Perplex - Tonight* Tantrum Desire - The Flow (Original Mix)* Mob Tactics - Pure X VIP (Original Mix)* REAPER & Josh Rubin - RUNAWAY* Paperclip - Koef* Krispe - Click Clack* Wilkinson - Frontline (Original Mix)* Lateral - Do It* Pythius - Byss (Original Mix)* Wilkinson - Dirty Love Feat. Talay Riley (Original Mix) * Wilkinson - Direction VIP * Dimension (UK) - Whip Slap (Original Mix) * Dimension - Crowd Reaction VIP * Dimension - UK* Gene Farris, Sub Focus - It's Time (Original Mix)* Levela, Waeys - Gasp (Original Mix)* Perplex - Malfunction* Intercept - Narcos* Wilkinson - Decompression (Original Mix)* Wilkinson - Take You Higher (Original Mix) Tracklisting:* Dan Guidance, Alex Barton - Waiting (Original Mix)* Nelver & Nic ZigZag - Ember Lights* FABRIC8 - Nobodys Love* Vektah - Pocket Rocket* V O E - Running Blind (Original Mix)* Justin Hawkes - Black Bloc (Original Mix)* Phibes - Forever (Original Mix)* Derwitch - Shona (Original Mix)* Bachelors Of Science feat. Pat Fulgoni - Stomp* Trex, Ella Jones, Trimer, Subten - Soul Food feat. Ella Jones (Original Mix)* Minor Forms, Kublai - Do Remember (Original Mix) * Spaow - Delta Warfare * Dr. Apollo - Holy Grail * Chase & Status, Liam Bailey - Blind Faith feat. Liam Bailey (Loadstar Remix) * Total Science, S.P.Y - Try Out feat. Marky* Black Sun Empire - Potemkin (S.P.Y Remix) (48)* Kove - Liberator (Original Mix) (32)* Trex - Dopamine (VIP)* Emba, L.I.T.A. - Holding Back (Original Mix)* DJ Wolf, Mojuba - Leaving Them Damaged - DEMO* Trex, X Bar Theory - Style & Substance (Club mix) feat. Trex (Beatport Exclusive)* Horge X Charles Rebellion - Ghost in the Shell * Unglued - Ichnusa* DC Breaks - Euphorium (Original Mix)* Agressor Bunx - Inner Line* Sub Focus - Off The Ground (Original Mix)* In:Most - Domino Effect* Grafix - Only Now* Delta Heavy - Heartbeat (Extended Mix)* Teddy Killerz - Night Train (Original Mix)* Noisia & IMANU - Shift* Anthropic - Sinistre* Bensley - Hit The Lights (Original Mix)* Demo - Run Them* DJ Hybrid, Trex - Comback (Trex Remix)* Demo - Music Maker (broken)* Vektah - Spectral (broken)* Damian Marley - Welcome to Jamrock (Conrad Subs Bootleg)* Rider Shafique, Trex - Undergrowth (Original Mix)* Prolix & Biometrix - Addicted To The Game* Lateral - BACKHOME* Grafix - CTRL* Sub Focus - Rock It (Wilkinson Remix)* Perplex - Never Stop* Mob Tactics - Wolf Trap * Dose, Teknik, JABZ MC - No Turning Back feat. Teknik feat. Jabz MC (Original Mix) * Skellytn - Particle Beam (ft. MagMag)* Woofax - Kick (Original Mix)* Perplex - Tonight* Tantrum Desire - The Flow (Original Mix)* Mob Tactics - Pure X VIP (Original Mix)* REAPER & Josh Rubin - RUNAWAY* Paperclip - Koef* Krispe - Click Clack* Wilkinson - Frontline (Original Mix)* Lateral - Do It* Pythius - Byss (Original Mix)* Wilkinson - Dirty Love Feat. Talay Riley (Original Mix) * Wilkinson - Direction VIP * Dimension (UK) - Whip Slap (Original Mix) * Dimension - Crowd Reaction VIP * Dimension - UK* Gene Farris, Sub Focus - It's Time (Original Mix)* Levela, Waeys - Gasp (Original Mix)* Perplex - Malfunction* Intercept - Narcos* Wilkinson - Decompression (Original Mix)* Wilkinson - Take You Higher (Original Mix) Download, Distribute, and Donate!

« Un coup de pied dans les urnes », c'est le podcast de Madmoizelle où Maëlle Le Corre, journaliste société, décrypte la présidentielle de 2022. Cette semaine, elle analyse les déclarations du président sur les 45h pour les jeunes.Crédits :Un coup de pied dans les urnes est un podcast de Madmoizelle écrit et présenté par Maëlle Le Corre. Réalisation, musique et édition : Mathis Grosos. Rédaction en chef : Mymy Haegel. Direction de la rédaction : Mélanie Wanga. Direction générale : Marine Normand. Voir Acast.com/privacy pour les informations sur la vie privée et l'opt-out.

« Un coup de pied dans les urnes », c'est le podcast de Madmoizelle où Maëlle Le Corre, journaliste société, décrypte la présidentielle de 2022. Cette semaine, elle analyse les déclarations du président sur les 45h pour les jeunes.Crédits :Un coup de pied dans les urnes est un podcast de Madmoizelle écrit et présenté par Maëlle Le Corre. Réalisation, musique et édition : Mathis Grosos. Rédaction en chef : Mymy Haegel. Direction de la rédaction : Mélanie Wanga. Direction générale : Marine Normand. Voir Acast.com/privacy pour les informations sur la vie privée et l'opt-out.

https://globalbusinessnews.net/wp-content/uploads/2021/10/Earth-Loop.mp4

November 5, 2021 ~ The Delta Dental Vice-President of Strategic Partnerships tells Paul they will be unveiling a new float this year.

Margaret Trimer has created a varied and fascinating career with a focus on building educational opportunities for children and girding communities with value and voice. She does this, she says, by following her personal core values.  Beginning at the Detroit Free Press as a reporter covering education, Margaret has moved through roles as founder and director of one of the highest performing charter school districts in the region, president and CEO of Junior Achievement of Southeast Michigan, communications director at the Michigan Education Association and most recently, as VP of Strategic Partnerships of Delta Dental of Michigan, Ohio and Indiana. In the latest episode of the Make Meaning Podcast, host Lynne Golodner speaks with Margaret about corporate caring for communities, what it takes to run extraordinary schools, and the beauty of driving across America with time to enjoy the scenery.

Natalie Anwar is a Michigan artist and single mom raising five boys. Three of them, 14-year-old triplets, are living with severe autism. They have limited verbal skills; they are one another's triggers. Some days these triggers lead to aggressive behavior or even physical violence.Some days, Anwar has to wear a helmet.Anwar holds her painting of her triplet sons in their favorite place—the water.Anwar relies on routine and being overly prepared for any situation. She also relies on public outdoor spaces like beaches and parks as well as public indoor spaces like museums in order to take her boys to experience the world and practice interacting with others. This past year, with access to public spaces limited or at times nonexistent, life has been particularly hard for Anwar and her family.“When everything went into lockdown, my world seemed like it was falling in front of my eyes, because I need a strong society for my children to get better,” Anwar said.Margaret Trimer, Vice President of Strategic Partnerships for Delta Dental of Michigan, met Anwar at a sensory-friendly event for families with children with autism last year—Delta Dental supports these events in partnership with Autism Alliance of Michigan. Trimer recently caught up with Anwar for the second episode in this podcast series.

KB connects with Margaret Trimer, who is navigating the strategic partnerships for Delta Dental in Michigan, Ohio and Indiana and she's getting ready to launch her own podcast in early March!

KB connects with Margaret Trimer, who is navigating the strategic partnerships for Delta Dental in Michigan, Ohio and Indiana and she's getting ready to launch her own podcast in early March!

01. Feint, Andromedik, Cammie Robinson - I'Ll Be There 02. Mountain - House (Dossa & Locuzzed Remix) 03. Nocturnum feat. Gabriel Eli - Silhouette (feat. Gabriel Eli) (Toronto Is Broken Remix) 04. Nct, Futurebound - The Feeling 05. Showdown - Balance 06. Quadrant, Iris - Stoic 07. Kanine, T-Man - All Goes Wrong 08. Dj Trace - Shack 09. Machinecode - Mockingbird 10. Technical Itch - Alien 11. Technical Itch - Belief 12. Ram Trilogy - No Reality (Andy C Remix) 13. Filip Motovunski, Joe Raygun - Serious Music 14. Dyslexia-Validol 15. Dorian - Radiation 16. Tobax - Autobahn 17. Mean Teeth - Dust To Dust 18. Ram Trilogy - Beastman (Traumatize Remix) 19. Zombie Cats - Grrrrr 20. Mean Teeth & Volatile Cycle - Bring It 21. Droptek - The Expanse [Mean Teeth Remix] 22. Drimuzz - Unknown Signal 23. Mean Teeth - Keepin It Dirty 24. Jabba - Bound Up 25. Mean Teeth & Nickbee - Critical Silence 26. Neonlight - Uhrwerk (Cuckoo Vip) 27. Grim Hellhound - Difficult Conversation 28. Volatile Cycle & Creatures - Purify 29. Drimuzz - Mystery Box 30. Division - In My Mind 31. Trex, Trimer, Pastry Maker - Isolation 32. Amoss - Speed Shades [Grey Code Remix] 33. Jayfor - Techno Biscuit 34. Droptek - Symbiosis [Ac13 Remix] 35. Endorphine - Automate 36. Seibel - The Dna 37. Bru-C, Devilman, Mr Traumatik - Volkswagen 38. Drimuzz - Alien Force 39. Profile & Sub Killaz - Third Dimension 40. Exile - Heat Friction 41. Metal Work - The Lion 42. Wcry - Oversight 43. Jeopardize - Manhunt (Vip) 44. The Zanoza - Why 45. Bru-C, Zero, Double L - Full Time Raver 46. Disrupta & Magenta - Mind Control 47. Kastro - Volatile 48. Nick The Lot - Catch Your Breath (Alex Slk Remix) 49. Trex, Gardna - R U Rollin 50. Bru-C - Mission 51. Mistic - The Yard 52. Azepct -The Fight 53. Gold Dubs, Fleck, Cheshire Cat - Rise Up 54. Chopstick Dubplate, Blackout Ja, Emma - Youthman (Nightshift Remix) 55. Dub Elements. -Southern Heat 56. Gold Dubs - The Jungle Is Changing 57. Kasra - I Dont Know What The Future Brings 58. Jammez - Flammable 59. Subview - All I Want 60. High Contrast feat. Uk Apache & Ady Suleiman - Met Her At A Dance In Leicester 61. High Contrast - Windows 95

01. Feint, Andromedik, Cammie Robinson - I'Ll Be There 02. Mountain - House (Dossa and Locuzzed Remix) 03. Nocturnum feat. Gabriel Eli - Silhouette (feat. Gabriel Eli) (Toronto Is Broken Remix) 04. Nct, Futurebound - The Feeling 05. Showdown - Balance 06. Quadrant, Iris - Stoic 07. Kanine, T-Man - All Goes Wrong 08. Dj Trace - Shack 09. Machinecode - Mockingbird 10. Technical Itch - Alien 11. Technical Itch - Belief 12. Ram Trilogy - No Reality (Andy C Remix) 13. Filip Motovunski, Joe Raygun - Serious Music 14. Dyslexia-Validol 15. Dorian - Radiation 16. Tobax - Autobahn 17. Mean Teeth - Dust To Dust 18. Ram Trilogy - Beastman (Traumatize Remix) 19. Zombie Cats - Grrrrr 20. Mean Teeth and Volatile Cycle - Bring It 21. Droptek - The Expanse [Mean Teeth Remix] 22. Drimuzz - Unknown Signal 23. Mean Teeth - Keepin It Dirty 24. Jabba - Bound Up 25. Mean Teeth and Nickbee - Critical Silence 26. Neonlight - Uhrwerk (Cuckoo Vip) 27. Grim Hellhound - Difficult Conversation 28. Volatile Cycle and Creatures - Purify 29. Drimuzz - Mystery Box 30. Division - In My Mind 31. Trex, Trimer, Pastry Maker - Isolation 32. Amoss - Speed Shades [Grey Code Remix] 33. Jayfor - Techno Biscuit 34. Droptek - Symbiosis [Ac13 Remix] 35. Endorphine - Automate 36. Seibel - The Dna 37. Bru-C, Devilman, Mr Traumatik - Volkswagen 38. Drimuzz - Alien Force 39. Profile and Sub Killaz - Third Dimension 40. Exile - Heat Friction 41. Metal Work - The Lion 42. Wcry - Oversight 43. Jeopardize - Manhunt (Vip) 44. The Zanoza - Why 45. Bru-C, Zero, Double L - Full Time Raver 46. Disrupta and Magenta - Mind Control 47. Kastro - Volatile 48. Nick The Lot - Catch Your Breath (Alex Slk Remix) 49. Trex, Gardna - R U Rollin 50. Bru-C - Mission 51. Mistic - The Yard 52. Azepct -The Fight 53. Gold Dubs, Fleck, Cheshire Cat - Rise Up 54. Chopstick Dubplate, Blackout Ja, Emma - Youthman (Nightshift Remix) 55. Dub Elements. -Southern Heat 56. Gold Dubs - The Jungle Is Changing 57. Kasra - I Dont Know What The Future Brings 58. Jammez - Flammable 59. Subview - All I Want 60. High Contrast feat. Uk Apache and Ady Suleiman - Met Her At A Dance In Leicester 61. High Contrast - Windows 95

01. Feint, Andromedik, Cammie Robinson - I'Ll Be There 02. Mountain - House (Dossa & Locuzzed Remix) 03. Nocturnum feat. Gabriel Eli - Silhouette (feat. Gabriel Eli) (Toronto Is Broken Remix) 04. Nct, Futurebound - The Feeling 05. Showdown - Balance 06. Quadrant, Iris - Stoic 07. Kanine, T-Man - All Goes Wrong 08. Dj Trace - Shack 09. Machinecode - Mockingbird 10. Technical Itch - Alien 11. Technical Itch - Belief 12. Ram Trilogy - No Reality (Andy C Remix) 13. Filip Motovunski, Joe Raygun - Serious Music 14. Dyslexia-Validol 15. Dorian - Radiation 16. Tobax - Autobahn 17. Mean Teeth - Dust To Dust 18. Ram Trilogy - Beastman (Traumatize Remix) 19. Zombie Cats - Grrrrr 20. Mean Teeth & Volatile Cycle - Bring It 21. Droptek - The Expanse [Mean Teeth Remix] 22. Drimuzz - Unknown Signal 23. Mean Teeth - Keepin It Dirty 24. Jabba - Bound Up 25. Mean Teeth & Nickbee - Critical Silence 26. Neonlight - Uhrwerk (Cuckoo Vip) 27. Grim Hellhound - Difficult Conversation 28. Volatile Cycle & Creatures - Purify 29. Drimuzz - Mystery Box 30. Division - In My Mind 31. Trex, Trimer, Pastry Maker - Isolation 32. Amoss - Speed Shades [Grey Code Remix] 33. Jayfor - Techno Biscuit 34. Droptek - Symbiosis [Ac13 Remix] 35. Endorphine - Automate 36. Seibel - The Dna 37. Bru-C, Devilman, Mr Traumatik - Volkswagen 38. Drimuzz - Alien Force 39. Profile & Sub Killaz - Third Dimension 40. Exile - Heat Friction 41. Metal Work - The Lion 42. Wcry - Oversight 43. Jeopardize - Manhunt (Vip) 44. The Zanoza - Why 45. Bru-C, Zero, Double L - Full Time Raver 46. Disrupta & Magenta - Mind Control 47. Kastro - Volatile 48. Nick The Lot - Catch Your Breath (Alex Slk Remix) 49. Trex, Gardna - R U Rollin 50. Bru-C - Mission 51. Mistic - The Yard 52. Azepct -The Fight 53. Gold Dubs, Fleck, Cheshire Cat - Rise Up 54. Chopstick Dubplate, Blackout Ja, Emma - Youthman (Nightshift Remix) 55. Dub Elements. -Southern Heat 56. Gold Dubs - The Jungle Is Changing 57. Kasra - I Dont Know What The Future Brings 58. Jammez - Flammable 59. Subview - All I Want 60. High Contrast feat. Uk Apache & Ady Suleiman - Met Her At A Dance In Leicester 61. High Contrast - Windows 95

01. Feint, Andromedik, Cammie Robinson - I'Ll Be There 02. Mountain - House (Dossa & Locuzzed Remix) 03. Nocturnum feat. Gabriel Eli - Silhouette (feat. Gabriel Eli) (Toronto Is Broken Remix) 04. Nct, Futurebound - The Feeling 05. Showdown - Balance 06. Quadrant, Iris - Stoic 07. Kanine, T-Man - All Goes Wrong 08. Dj Trace - Shack 09. Machinecode - Mockingbird 10. Technical Itch - Alien 11. Technical Itch - Belief 12. Ram Trilogy - No Reality (Andy C Remix) 13. Filip Motovunski, Joe Raygun - Serious Music 14. Dyslexia-Validol 15. Dorian - Radiation 16. Tobax - Autobahn 17. Mean Teeth - Dust To Dust 18. Ram Trilogy - Beastman (Traumatize Remix) 19. Zombie Cats - Grrrrr 20. Mean Teeth & Volatile Cycle - Bring It 21. Droptek - The Expanse [Mean Teeth Remix] 22. Drimuzz - Unknown Signal 23. Mean Teeth - Keepin It Dirty 24. Jabba - Bound Up 25. Mean Teeth & Nickbee - Critical Silence 26. Neonlight - Uhrwerk (Cuckoo Vip) 27. Grim Hellhound - Difficult Conversation 28. Volatile Cycle & Creatures - Purify 29. Drimuzz - Mystery Box 30. Division - In My Mind 31. Trex, Trimer, Pastry Maker - Isolation 32. Amoss - Speed Shades [Grey Code Remix] 33. Jayfor - Techno Biscuit 34. Droptek - Symbiosis [Ac13 Remix] 35. Endorphine - Automate 36. Seibel - The Dna 37. Bru-C, Devilman, Mr Traumatik - Volkswagen 38. Drimuzz - Alien Force 39. Profile & Sub Killaz - Third Dimension 40. Exile - Heat Friction 41. Metal Work - The Lion 42. Wcry - Oversight 43. Jeopardize - Manhunt (Vip) 44. The Zanoza - Why 45. Bru-C, Zero, Double L - Full Time Raver 46. Disrupta & Magenta - Mind Control 47. Kastro - Volatile 48. Nick The Lot - Catch Your Breath (Alex Slk Remix) 49. Trex, Gardna - R U Rollin 50. Bru-C - Mission 51. Mistic - The Yard 52. Azepct -The Fight 53. Gold Dubs, Fleck, Cheshire Cat - Rise Up 54. Chopstick Dubplate, Blackout Ja, Emma - Youthman (Nightshift Remix) 55. Dub Elements. -Southern Heat 56. Gold Dubs - The Jungle Is Changing 57. Kasra - I Dont Know What The Future Brings 58. Jammez - Flammable 59. Subview - All I Want 60. High Contrast feat. Uk Apache & Ady Suleiman - Met Her At A Dance In Leicester 61. High Contrast - Windows 95

Recorded 2020-12-02 05:07:34 Tracklisting: * Honey Bunny - Sunset In Ibiza (Original mix) * Keeno - Antiphony * Flawoh - Forever * Monologue, Roygreen & Protone - Whisper * Shivaxi, InTake - Enduring * Joakuim, Roygreen & Protone - Clarksdale * Kastro - Rooftop Melodies * K-Warren, Benny V - Cover It Up * Funkware - It Works * Hugh Hardie, DJ Marky feat. Cimone - Said & Done * Koherent - Bliss * Kolectiv, Becca Jane Grey - Give Nothing feat. Becca Jane Grey (Wingz Remix) * Workforce - Your Loss (Halogenix Remix) * Trex, Ella Jones - Dreamy Dayz feat. Ella Jones * Stillz - See No Evil * Meladee - Astrocone * Conrad Subs - Evergreen * GLM - Mercy * Khemikal - Goin' In * Wingz - IDW * Lowriderz & Outselect - Next Level * Covert Garden - Dead Zone * Itmek, Skudkid - Learn to fly * Wingz - Rearranged * Tesen - Wookie * Erritate - Polar * Feed The Fire - Immortals * Kodin - Chronic * Simula - Apollo 17 * Notequal - Synchronen * Stillz - Ever In Me * Monika, Chisel - Pull Up!! * Genic, Sensus - Sensible * Half Cab, DIS:TURBED - Get Down * MC Bassman, Simula - Break Em Down feat. MC Bassman * Erritate - The Birdman * Forbidden Society - Bottom Check (Original) * Inflamers - Bass in the Staircase * Stillz - Lies * Tesen - Lose It * Tesen - Final Straw * Frankee - Pandorum * Muffler - Watchdog * Tsuki - Bring the Sound * Cutworx - Words (Zero T Remix) * Hilleh - Elementz * Jappa, Bunnerz - Pretend * Kaiza, Xeomi - Deeper * Systm Hackerz - Bad Man * The Skeptics - There Abouts * Trex, Trimer, Pastry Maker - Isolation feat. Trimer feat. Pastry Maker * Systm Hackerz - Kebab Shop Banter * Amplify - Present * Stillz - Condition Red * Filthy Habits - Wasted (Puppetz Remix) * Katharsys - Slippery Slope [Current Value Remix] * Jedi - No Idea * KL - Radiant * Harley D - Distress Signal * Maze - Impatience * Laney, Kendrick - One on One (Kendrick Remix) * Ky - Untouched * Ky - Stranger * Ben Snow - Out In The Streets (Voltage Remix) * Gino, Warhead - By The Books * Mark Dinimal - Rasputin * Jeopardize - Technique * Deekline, Specimen A, Killa P, Sweetie Irie, Brian Brainstorm, MC B-Live - Kill That Sound (Benny Page Remix) * Java - Rudeboy * Fleck - Lover * Jaybee - The Day After Thursday * Emma, Blackout Ja, Chopstick Dubplate - Youthman * Sound In Noise - Duppy Bass VIP * Kumarachi - Never Stop * Keeno, Obsel - Fear * Kontakt - Planet 9 * Giganti - The Kraken * Proper Villains - Sound System (Craze Remix) * Thing - Just A Track Download, Distribute, and Donate!

01 - 00:00:00 - Physics - Dark Waters 02 - 00:00:51 - Mystific and Jungie - Never Alone 03 - 00:03:35 - FX909 - And she lies 04 - 00:06:25 - Bou - Wine Ya B 05 - 00:09:07 - Jeddy Beats - Flite 06 - 00:11:12 - Krust - Warhead 07 - 00:14:12 - Streetz Of Rage - Red Queen 08 - 00:15:51 - SUNCHASE - Step Outside 09 - 00:18:34 - WORKFORCE - Your Loss (Halogenix remix) 10 - 00:22:06 - Break, DLR - Hit the Target 11 - 00:24:40 - DJ Randall - Lighthouse Connect 12 - 00:26:00 - Alix Perez, Halogenix - Unlustrous 13 - 00:27:47 - Logistics, Fred V - You Keep Me High 14 - 00:29:39 - Myth - One Note Funk 15 - 00:32:49 - Reasxn - Operate 16 - 00:34:06 - WORKFORCE - Overnight Express (feat SP:MC (Break remix)) 17 - 00:38:35 - Chromatic - Switcher 18 - 00:39:48 - Cutworx - Words (Zero T remix) 19 - 00:40:50 - BUNGLE - Unlocked 20 - 00:43:12 - Marcus Intalex & ST Files - My Soul 21 - 00:46:17 - CPH and Silence Groove - Subdued 22 - 00:49:23 - SAL - Beskar 23 - 00:50:42 - Streetz Of Rage - Unfamiliar Wayz 24 - 00:53:19 - QZB - Apollo 25 - 00:55:10 - Kiril - Untitled Dub 26 - 00:57:12 - Ed Rush & Optical - Mystery Machine 27 - 01:00:08 - NC-17, Dave Owen - Deviant 28 - 01:02:43 - ShockOne - Follow Me 29 - 01:04:57 - Levela, Teddy Killerz, Gydra - Miles High (Levela Remix) 30 - 01:07:27 - Sustance - Be Forgiven 31 - 01:10:12 - NC-17, Dave Owen, Black Opps - Night Of The Creeps 32 - 01:12:05 - DJ SS, Streetz Of Rage - Antidote (feat. DJ SS) 33 - 01:13:22 - TREX. - Isolation (feat Trimer & Pastry Maker) 34 - 01:15:59 - MACHINEDRUM/HOLLY - Berry Patch 35 - 01:17:31 - Data - The Causeway (Commix remix) 36 - 01:20:21 - Picota & Kumbh - Sacrifice 37 - 01:22:10 - Optical + Ryme Tyme - Ghostbuster 38 - 01:24:22 - Koven - Followers (A.M.C Remix) 39 - 01:27:04 - Signal - Ennair 40 - 01:28:37 - NC-17 - Cemetery of Splendour 41 - 01:31:29 - Mylo, Claptone - Drop The Pressure (1991 Remix Extended Version) 42 - 01:32:55 - Break - The Truth 43 - 01:36:06 - The Upbeats & Noisia - Clamber 44 - 01:37:11 - Bad Company - Sentient 45 - 01:39:56 - SKC And Safair - Free My Soul 46 - 01:42:22 - Smooth - Timeless 47 - 01:45:44 - Kiril - MKD 48 - 01:48:30 - Upgrade - Gangbeasts 49 - 01:49:22 - Jo - R-Type 50 - 01:51:11 - Noisia & Prolix - Asteroids 51 - 01:54:00 - Bad Company - Brain-Scan

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2020.09.21.306357v1?rss=1 Authors: Ma, J., Su, D., Huang, X., Liang, Y., Ma, Y., Liang, P., Zheng, S. Abstract: Less than a year after its emergence, the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has infected over 22 million people worldwide with a death toll approaching 1 million. Vaccination remains the best hope to ultimately put this pandemic to an end. Here, using Trimer-Tag technology, we produced both wild-type (WT) and furin site mutant (MT) S-Trimers for COVID-19 vaccine studies. Cryo-EM structures of the WT and MT S-Trimers, determined at 3.2 Angstrom and 2.6 Angstrom respectively, revealed that both antigens adopt a tightly closed conformation and their structures are essentially identical to that of the previously solved full-length WT S protein in detergent. These results validate Trimer-Tag as a platform technology in production of metastable WT S-Trimer as a candidate for COVID-19 subunit vaccine. Copy rights belong to original authors. Visit the link for more info

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2020.08.21.261974v1?rss=1 Authors: Jette, C. A., Barnes, C. O., Kirk, S. M., Melillo, B., Smith, A. B., Bjorkman, P. J. Abstract: Human Immunodeficiency Virus-1 (HIV-1), the causative agent of AIDS, impacts millions of people. Entry into target cells is mediated by the HIV-1 envelope (Env) glycoprotein interacting with host receptor CD4, which triggers conformational changes allowing binding to a coreceptor and subsequent membrane fusion. Small molecule or peptide CD4-mimetic drugs mimic CD4's Phe43 interaction with Env by inserting into the conserved Phe43 pocket on Env subunit gp120. Here, we present single-particle cryo-EM structures of CD4-mimetics BNM-III-170 and M48U1 bound to a BG505 native-like Env trimer plus the CD4-induced antibody 17b at 3.7[A] and 3.9[A] resolution, respectively. CD4-mimetic-bound BG505 exhibits canonical CD4-induced conformational changes including trimer opening, formation of the 4-stranded gp120 bridging sheet, displacement of the V1V2 loop, and formation of a compact and elongated gp41 HR1C helical bundle. We conclude that CD4-induced structural changes on both gp120 and gp41 Env subunits are induced by binding to the gp120 Phe43 pocket. Copy rights belong to original authors. Visit the link for more info

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2020.08.11.245696v1?rss=1 Authors: Zhang, K., Li, S., Pintilie, G., Chmielewski, D., Schmid, M. F., Simmons, G., Jin, J., Chiu, W. Abstract: Human coronavirus NL63 (HCoV-NL63) is an enveloped pathogen of the family Coronaviridae that spreads worldwide and causes up to 10% of all annual respiratory diseases. HCoV-NL63 is typically associated with mild upper respiratory symptoms in children, elderly and immunocompromised individuals. It has also been shown to cause severe lower respiratory illness. NL63 shares ACE2 as a receptor for viral entry with SARS-CoV and SARS-CoV-2. Here we present the in situ structure of HCoV-NL63 spike (S) trimer at 3.4-[A] resolution by single-particle cryo-EM imaging of vitrified virions without chemical fixative. It is structurally homologous to that obtained previously from the biochemically purified ectodomain of HCoV-NL63 S trimer, which displays a 3-fold symmetric trimer in a single conformation. In addition to previously proposed and observed glycosylation sites, our map shows density at other amino acid positions as well as differences in glycan structures. The domain arrangement within a protomer is strikingly different from that of the SARS-CoV-2 S and may explain their different requirements for activating binding to the receptor. This structure provides the basis for future studies of spike proteins with receptors, antibodies, or drugs, in the native state of the coronavirus particles. Copy rights belong to original authors. Visit the link for more info

Этот взрывной и 888ой эпизод Пиратской Станции был раскачан речитативом SMOKY D из Питера, который почитал треки из своего нового альбома, разогрел атмосферу и в целом показал свой профессиональный скилл как эмси! Эфир продолжился звездопадом ядерных танцевальных вещей, смешанных мной с любовью к drumandbass! Не останавливайте музыку.... MC SMOKY D rhymes: + Outselect - Trust The Beat + Lowriderz - No Hate + Lowriderz - Jungle Representah + No Limit & Shiny Radio - My Dedication GVOZD vibes: 1.Turno, Upgrade, Logan D - Make It Clap (Low Down Deep) 2.DJ Phantasy, Macky Gee & MC Shabba D - We Love Drum & Bass (Sasasas Music) 3.Upgrade - Do It Good (Undiluted) 4.Mr Quiet, Sym-on - Day Dreaming (Stickybass) 5.Dist HarD - Bass Terror (Gysnoise dub) 6.Wild Boyz! - Wait (Play Me) 7.Falco & Dub Sense - Involved (Gully Beats) 8.Shimon, Trimer, Pastry Maker - Take Position (Audioporn) 9.Nymfo - Hot as Hell (Barong Family) 10.L Plus - Rocks (Technique) 11.Teddy Killerz - Monsters (Viper) 12.NCT, Franky Nuts, Coppa - Bad (Viper) 13.Myselor - Attitude (Red Light) 14.Mindscape feat. Fedora - Soul Eaters (Eatbrain) 15.Jade - TV Says (EatBrain) 16.Decent & Snapper - Make it Bounce (Respect dub) 17.Zardonic feat. Coppa - Children Of Tomorrow (Entertainment One) 18.Saharaksha & Bioglard - Ants Sadists (dub) 19.Andromedik - Low (Radar) 20.Bl4ck Owlz - Chillout (Bad Taste dub) 21.Razlom feat Asphexia - The Run (Empire) 22.Lockjaw, Current Value - Passing (Locked Concept) 23.Transforma - The Darkest Void (Bad Taste) 24.Noel - Vapid (Program) 25.D-Sabber - Numbers (Respect dub) 26.Netsky, A.Chal - Tequila Limonada (The Prototypes Remix) (Republic) 27.Metrik - Dawnbreaker (Hospital) 28.Changing Faces - Shadows (Spearhead) 29.Bou - Fever (Subway Sounds) 30.Section - We Need You (Locked Up Music) 31.Jam Thieves - Overdose (Playaz) 32.Document One - We Got This(Shogun audio) 33.Kusp - Shook (Detail) 34.Fox, Foreign Concept & Sam Binga - Simmer Down (Critical music) 35.Soul Connection - Stay True (Liquid Flavours) 36.Mr Explicit - Dub Lion (Liondub International) 37.Digital, Drum Cypha - Satsuma (Holotype Audio) 38.Jubei & Tyrone - Stabs (Metalheadz) 39.Tweakz - The Shimmer (Syncopix) 40.Sonic Art - Time & Space (Greekboy Remix)(Kos.Mos.Music) 41.Redeyes, Tyler Daley - Late Night Jam (The North Quarter) 42.Spectrasoul, LSB - Silence (Ish Chat Music) 43.Dub Defense - Writing (Liquid Drops) 44.Malbeat - Autumn (dub) 45.Commix - The Time (Metalheadz) 46.Alex SLK - Controller (Citate Forms dub) 47.Quartz & Survey - Gloop (Warm Communications) 48.Kyrist - Fragment (Dispatch) 49.Kung - NTSC (Program) 50.Thirteenth Output - Moon (Universe Axiom)

Этот взрывной и 888ой эпизод Пиратской Станции был раскачан речитативом SMOKY D из Питера, который почитал треки из своего нового альбома, разогрел атмосферу и в целом показал свой профессиональный скилл как эмси! Эфир продолжился звездопадом ядерных танцевальных вещей, смешанных мной с любовью к drumandbass! Не останавливайте музыку.... MC SMOKY D rhymes: + Outselect - Trust The Beat + Lowriderz - No Hate + Lowriderz - Jungle Representah + No Limit & Shiny Radio - My Dedication GVOZD vibes: 1.Turno, Upgrade, Logan D - Make It Clap (Low Down Deep) 2.DJ Phantasy, Macky Gee & MC Shabba D - We Love Drum & Bass (Sasasas Music) 3.Upgrade - Do It Good (Undiluted) 4.Mr Quiet, Sym-on - Day Dreaming (Stickybass) 5.Dist HarD - Bass Terror (Gysnoise dub) 6.Wild Boyz! - Wait (Play Me) 7.Falco & Dub Sense - Involved (Gully Beats) 8.Shimon, Trimer, Pastry Maker - Take Position (Audioporn) 9.Nymfo - Hot as Hell (Barong Family) 10.L Plus - Rocks (Technique) 11.Teddy Killerz - Monsters (Viper) 12.NCT, Franky Nuts, Coppa - Bad (Viper) 13.Myselor - Attitude (Red Light) 14.Mindscape feat. Fedora - Soul Eaters (Eatbrain) 15.Jade - TV Says (EatBrain) 16.Decent & Snapper - Make it Bounce (Respect dub) 17.Zardonic feat. Coppa - Children Of Tomorrow (Entertainment One) 18.Saharaksha & Bioglard - Ants Sadists (dub) 19.Andromedik - Low (Radar) 20.Bl4ck Owlz - Chillout (Bad Taste dub) 21.Razlom feat Asphexia - The Run (Empire) 22.Lockjaw, Current Value - Passing (Locked Concept) 23.Transforma - The Darkest Void (Bad Taste) 24.Noel - Vapid (Program) 25.D-Sabber - Numbers (Respect dub) 26.Netsky, A.Chal - Tequila Limonada (The Prototypes Remix) (Republic) 27.Metrik - Dawnbreaker (Hospital) 28.Changing Faces - Shadows (Spearhead) 29.Bou - Fever (Subway Sounds) 30.Section - We Need You (Locked Up Music) 31.Jam Thieves - Overdose (Playaz) 32.Document One - We Got This(Shogun audio) 33.Kusp - Shook (Detail) 34.Fox, Foreign Concept & Sam Binga - Simmer Down (Critical music) 35.Soul Connection - Stay True (Liquid Flavours) 36.Mr Explicit - Dub Lion (Liondub International) 37.Digital, Drum Cypha - Satsuma (Holotype Audio) 38.Jubei & Tyrone - Stabs (Metalheadz) 39.Tweakz - The Shimmer (Syncopix) 40.Sonic Art - Time & Space (Greekboy Remix)(Kos.Mos.Music) 41.Redeyes, Tyler Daley - Late Night Jam (The North Quarter) 42.Spectrasoul, LSB - Silence (Ish Chat Music) 43.Dub Defense - Writing (Liquid Drops) 44.Malbeat - Autumn (dub) 45.Commix - The Time (Metalheadz) 46.Alex SLK - Controller (Citate Forms dub) 47.Quartz & Survey - Gloop (Warm Communications) 48.Kyrist - Fragment (Dispatch) 49.Kung - NTSC (Program) 50.Thirteenth Output - Moon (Universe Axiom)

Oh that new car smell! Yes, BTS 022 is here with the freshness!That’s right, nothing but new and fresh tunes right here to keep your car or wherever you maybe new and fresh! Go ahead and check the tracklist and you’ll see we have nothing but major forthcoming releases along with some great segments this week for the Best Drum and Bass podcast series. “Melissa’s music pick of the week” guarantee’s a killer combo of vibes and vocals. “All about the gems” feature a monster remix of a Stateside OG and “All things D&B” showcases another side of KNOXZ that some might not know but sure to get you amp for whats to come of the might American Grime gang.Ep 22 TracklistL 33 / So Dope (feat. Coppa) [Eatbrain]Des McMahon / Bulletproof [Santoku]Cynical Gene / Funk HustlerOptiv & BTK / Dive Bomb (Pythius Remix) [Dutty Audio]FuntCase & Teddy Killerz / You Must Comply [UKF]Optiv & BTK / Shredder (Inward, Hanzo & Randie aka IHR Remix) [Dutty Audio]The Clamps / Cattle Mutilation Syndrome [Kosen]L 33 / Mafia [Eatbrain]*Zhu / Faded (Delta Heavy) [Bootleg]The Clamps & Merikan / Fast Way [Kosen]L 33 / Lights Out (feat. Copper) [Eatbrain]KS FT. MC STERN / VIOLENCE [Abducted LTD]The Clamps & Mizo / Time Pressure [Kosen]The Clamps / The First and The Last [Kosen]KS / MutantThe Clamps & NickBee / Addicted [Kosen]Prolix / Beat Down feat. DC Breaks [Blackout Music NL]KNOXZ / Relentless [Renegade Hardware]*Karl K – Synapse (Konflict Remix) [Higher Education]L 33 / Last Signal [Eatbrain]Des McMahon / Ghost [Santoku]Sublimit / Tunnel Vision [Four Corners]Cynical Gene / Marching Forward [Sublimit / Mass Control [Four Corners]Sublimit / Sway [Four Corners]Shimon & Trimer feat. Pastry Maker / Take Position ft Pastry Maker [AudioPorn]Severity / Fakeworld [Unchained]Severity & Royalton / Fakeworld (Royalston Remix) [Unchained]Shimon & Trimer / Giant Locust [AudioPorn]Severity & Whiney / Broken Hearted (Whiney Remix) [Unchained]*Werd2Jah, DOS, Argyle Bixby, Jumanji, Knoxz, 3iJoe, Twisty / Best of the Best (3RDi Remix) [American Grime]*music separate from mix.Follow Knoxz: www.facebook.com/KNOXZMUSICwww.soundcloud.com/KNOXZListen to Best Drum and Bass Podcast – Beneath the Surface Ep. 22 on these platforms: iTunes – http://bit.ly/bestdnbGoogle Play – http://bit.ly/bdnb-gplay iHeartRadio – http://bit.ly/bdnb-ihrTunein – http://bit.ly/bdnb-tunein Stitcher – http://bit.ly/bdnb-stitcher See acast.com/privacy for privacy and opt-out information.

Music evolution & career facts – from Coppa's beginnings all the way to this day. Drum & bass podcast hosted by Andy @ 12pm GMT every other Sunday - see you next on 08/4/18! Previous episode '20 years of Virus': https://soundcloud.com/artsyaudio/20-years-of-virus-mix-artsy-audio-podcast-020 Connect with Artsy Audio: ● Facebook → http://www.facebook.com/artsyaudio ● Instagram → https://www.instagram.com/artsyaudio/ ● Soundcloud → http://www.soundcloud.com/artsyaudio ● iTunes → https://apple.co/2oZkThq ● Twitter → https://twitter.com/artsyaudio ● Web → http://www.artsyaudio.com Thanks for tuning in! :) Tracklist: 01:18 Beat Pharmact ft. Coppa – Rooftops (2009) 03:17 Break The Box ft. MC Coppa – Style And Flavour (2009) 07:15 Disaszt & Shimon ft. MC Coppa – Rumours (2010) 09:26 Afghan Headspin ft. MC Coppa – Going Down (2011) 11:20 Prolix ft. MC Coppa – On Like That (2012) 13:20 Mindscape & Jade ft. Coppa – Friday the 13th (2014) 15:46 Borderline ft. Coppa – 6 am (2015) 19:50 Coppa, Raise Spirit, Nihayet - Oh No (2015) 22:45 Mindscape ft. Coppa – We Set Fire (2015) 27:15 ●●● Talk on Coppa's Career Evolution 33:48 Tantrum Desire ft. Coppa – Repeat (2015) 36:48 Coppa ft. Kursiva – Demon Inside (2016) 40:10 Coppa– Skanka ft. Benny L (2018) 43:05 Coppa– Poetry in Motion ft. Madface (2018) 47:08 Coppa - Much to Them ft. Current Value (2018) 50:25 Coppa - Rhino ft. Bassbrothers (2018) 52:40 Coppa – Bring War ft. Shimon & Trimer (2018) 55:57 Coppa – Anything ft. High Maintenance (2018) 59:13 Coppa– 1000 miles ft. NCT (2018) Coppa links: ● Web → https://www.coppaworld.com/ ● Facebook → https://www.facebook.com/coppaworld/ ● Twitter → https://twitter.com/coppaworld ● Insta→ https://www.instagram.com/coppaworld/ We do not claim to own any of the songs played in this episode and they belong to their respective owners. No copyright infringement was intended. We do not receive income of any sort from this work. This podcast's sole intention is to promote the artists and their work. Music used in this episode has been legally purchased. If possible, always support artists by buying their work.

IT HAS ARRIVED, MC Coppa’s legendary level album “Poetry in Motion” on AudioPorn was just released, and its skyrocketed to the #1 position. With producers like Benny L, Current Value, Shimon, Trimer, L 33, Muffler and tons more, this release has something for everyone. I HIGHLY suggest you go check it out, as its awesome to see one of the best MC’s in Drum and Bass have a proper spotlight to push a full album.Buy Poetry In Motion LP by MC Coppa: https://smarturl.it/apornlp005Official Coppa / Poetry in motion mailing list http://www.coppadub.onlineSubscribe to the podcast on iTunes: http://bit.ly/bestdnbPodcast now available also on these platforms: Google Play | iHeartRadio | Tunein| StitcherBad Syntax TracklistSequential & Dizkret – Resonant Circuit [Abducted LTD]Tobax & Mean Teeth – Bring the Pain [C4C]AMC & Turno – Draw 4 That [D&B Arena]Teddy Killerz – Hellblade EB [Eatbrain]Void – Saws N Flutes [Mayan]Hanm & KS – Startdust [Abducted LTD]Akov – Initiation [Bad Taste]Tobax – From Within [C4C]Mayel – Runaway Train [Invasion]Prolix ft Ben Verse – Run the Beat [Trendkill]Dose – Birth By Design [Abducted LTD]QZB & Enei – Johannesburg [Critical]Rizzle – Teachings [Symmetry]Travesty – Enclosed VIP [Tytanium Armour]Akov – Talk to Me [C4C]Sequential & Dizkret – Spiral [Abducted LTD]AMC & Turno – Maybach [D&B Arena]Ephyum – Abracadabra [Kill Tomorrow]Tobax – The Alchemist [C4C]Cooh & L 33 – Broken [Othercide]Wresker & Kilobite – Capslock [Abducted LTD]QZB – Nobunaga [Critical]Mark Galaxy – Event Horizon [Eatbrain]DSP – The Mist [Symmetry]Instinkt – Ultra [Delta 9]Insom – Vacuum [Abducted LTD]Disazt ft North Base – The Life [Mainframe]Akov – Schnitzelklopfer [C4C]Humanon & Sineform – Gnosis [Kill Tomorrow]AMC & Turno – Alliance [D&B Arena]Dioptrics & RA:SKL – Maniac (Pish Posh RMX) [Abducted LTD]Disprove – Relative [Delta 9]Maztek & Gridlok – Gladiators [0101]KarmasynK – Paranormal [C4C]QZB – Ornstein [Critical]Bad Syntax & Pish Posh – Dark Planet [Abducted LTD]Bad Tuna Out NowDub Elements – Steamy [DEM]Task Horizon – Nstromo [Evolution Chamber]Mayel & Irontype – Mixture [Invasion]KarmasynK – Cant Cope [C4C]Void – New Machine [Mayan]Glyph – One Million Ants [Symmetry]Prolix – Lightspeed [Trendkill]Trimer – Poetry in Motion TracklistMampi Swift & Fresh – Play Me (Mob Tactics Remix)Coppa – Skanka ft Benny LShimon & Trimer – Scaredy CatCoppa – Much to Them fr Current ValueMampi Swift – The One (Inside info Remix)Coppa – Poetry in Motion ft MadfaceAkov – Growing PainsCoppa – Brainwashed ft Nymfo & Zero ZeroTrimer – MicroDotCoppa – 1000 Miles ft NCTBenny L & Shimon – SharksCoppa – Architects ft AlibiSerum – Soul TravellingCoppa – Reasons to Survive ft DexcellShimon & Trimer – ???Coppa – Pirate Beats fr SaxxonSammy Nicks & Rekwest – Eat Box (Voltage & Nicky Blackmarket Remix)Taxman – Evasion RemixDigital – Spacefunk (Nasty Habits remix)Sub Zero & Limited – VibrationsCoppa – Anything ft High MaintenanceSub Focus – AirplaneBuy Poetry In Motion LP by MC Coppa: https://smarturl.it/apornlp005Official Coppa / Poetry in motion mailing list http://www.coppadub.onlineFollow Abducted LTDFacebook: https://www.facebook.com/abductedltdBeatport: http://bit.ly/abductedltdDownload / Listen to our weekly podcasts (every friday)(Soundcloud) http://bit.ly/ltdpodcast or (iTunes) http://bit.ly/bestdnbFollow Bad SyntaxFacebook: facebook.com/badsyntaxdnbListen to the NOISIA RADIO world premier of Dose – Cultivate (Bad Syntax & HANM RMX)Download the stems to Bad Syntax & Hanm – Red Giant: https://soundcloud.com/abducted/sets/bad-syntax-hanm-red-giant-stem-giveawayKeep up to date on the Best Drum and BassFacebook: https://www.facebook.com/bestdrumandbassSubscribe on iTunes: http://bit.ly/bestdnbWeekly Broadcast Thursdays 9pm PST: https://www.twitch.tv/bestdrumandbasswww.bestdrumandbass.comPodcast now available also on these platforms: Google Play – http://bit.ly/bdnb-gplay iHeartRadio – http://bit.ly/bdnb-ihrTunein – http://bit.ly/bdnb-tunein Stitcher – http://bit.ly/bdnb-stitcherWatch Bad Syntax record his set LIVE on 3 decks! (NOW ON TWITCH!)Watch WERE LIVE! Recording my part of the official MC COPPA – POETRY IN MOTION official podcast! from bestdrumandbass on www.twitch.tv See acast.com/privacy for privacy and opt-out information.

No Shave November is about maturity.

Etwa 30% aller Gene codieren für Membranproteine (MP). Trotz ihrer hohen Relevanz, speziell im medizinischen Bereich, stellt die Analyse von MP aufgrund ihrer physikalisch-chemischen Eigenschaften ein häufiges Problem in der Proteinbiochemie dar. Diese Arbeit soll eine Einsicht in die Problematik geben sowie Lösungsansätze aufzeigen, um den Umgang mit diesen Polypeptiden zu vereinfachen. Ein geeignetes Modellsystem zum Studium der Eigenschaften membranintegraler Proteine und Peptide sowie zur Verbesserung der bestehenden Analysemethoden stellte die Thylakoidmembran der Plastide dar. Um das funktionelle Proteom der Thylakoidmembran zu definieren, wurden die Proteinkomplexe der Thylakoidmembran von Gerste (Hordeum vulgare) über hochauflösende 2D-Blue Native /SDS-Polyacrylamidgelelektrophorese (PAGE) getrennt. Das Gelsystem erlaubte die Isolation der photosynthetisch aktiven Proteinkomplexe PSI/LHCI, PSII, LHCII, Cytochrom b6/f und ATPase in unterschiedlichen Assemblierungszuständen. Im Fokus der Untersuchungen stand die Charakterisierung der isolierten Subkomplexe von PSII. Die Identifikation der Komplexuntereinheiten erfolgte nach enzymatischem In-Gel Verdau und massenspektrometrischer Analyse der entstandenen Peptide (offline nanoESI-MSMS). MP > 10 kDa wurden ausschließlich über Peptide aus den löslichen Abschnitten identifiziert. Die Analyse der niedermolekularen Untereinheiten (< 10 kDa) wurde auf Ebene des Gesamtproteins nach Extraktion aus den Komplexbanden der BN-PAGE realisiert. Dabei konnten dem mono- und dimeren PSII-Subkomplex folgende niedermolekularen UEn zugeordnet werden: PsbE, PsbF, PsbI, PsbK, PsbL, PsbM, PsbTc und PsbX. Da kein Unterschied in der Zusammensetzung des mono- und dimeren PSII-Subkomplexes existierte, konnte eine Beteiligung einer der niedermolekularen UEn an der Ausbildung des dimeren PSII-Subkomplexes im Rahmen der Assemblierung nicht bestätigt werden. Die Lichtsammelproteine (LHCP) des LHCII wurden nach 2D BN/SDS-PAGE auf Ebene der Superkomplexe oder abgetrennt als Mono- und Trimerer LHCII-Subkomplex identifiziert, wobei das Trimer durch das Fehlen der minoren LHCP (CP29, CP26 und CP24) charakterisiert war. Die für Membranproteine der Thylakoide ungewöhnlich hydrophilen LHCP erhielten die benötigte Hydrophobizität zur Durchspannung der Membran über die Bindung von Pigmenten (Chlorophyll). Eine eindeutige Unterscheidung der Genprodukte von Lhcb1-3 war trotz extremer Sequenzhomologie über die Detektion eines charakteristischen Peptids im N-terminalen Bereich der maturen Sequenz möglich. In Gerste wurde somit jeweils eine Form von Lhcb2 und 3, sowie sechs Isoformen von Lhcb1 identifiziert. Um den In-Gel Verdau von Proteinen nach elektrophoretischer Trennung zu vereinfachen und zu standardisieren, wurde ein Reaktionsgefäß (OMX-S®) aus Polypropylen entwickelt. Im Zuge der Anpassung des konventionellen Protokolls zum In-Gel Verdau von Proteinen für OMX-S® wurde ein optimiertes Verdauprotokoll entwickelt, das ohne die Reaktionsschritte Entfärbung, Reduktion & Alkylierung der AS Cystein sowie eine multiple Extraktion zur Anreicherung der entstandenen Peptide auskommt. Die Erhöhung der Reaktionstemperatur auf 50°C und die Verkürzung der Diffusionsstrecke für die Protease erhöhten zudem die Effizienz des Verdaus und führten zu einer Reduktion der gesamten Prozesszeit von 6-24 h auf 1 h. Welche Auswirkung die Auslassung einzelner Reaktionsschritte auf die Peptidausbeute hatte, wurde nach differentieller Isotopenmarkierung der generierten Peptide mittels massenspektrometrischer Analyse quantifiziert. Da jeder Prozessierungsschritt eine potentielle Quelle für Verluste darstellte, waren die Peptidausbeuten im Vergleich zum konventionellen In-Gel Verdau äquivalent oder sogar besser. Unabhängig vom verwendeten Verfahren, fehlten die membranintegralen Peptide in den Spektren. Folglich wurde die Detektierbarkeit und Signalintensität von tryptischen Peptiden in Abhängigkeit von verschiedenen Faktoren untersucht. Dabei ergab sich eine direkte Korrelation zwischen der Proteinmenge einer Bande und der Anzahl, der nach Verdau detektierten Peptide. Die Untersuchungen an Peptiden aus löslichen und membranintegralen Proteinen ergaben, dass die Hauptursache für das Fehlen letzterer, nicht auf den Einfluss bestimmter AS auf die Ionisierbarkeit, die Sequenzlänge und/oder die Hydrophobizität zurückzuführen war. Entscheidend für die Abwesenheit der membranintegralen Peptide war vielmehr die schlechte Zugänglichkeit der Schnittstellen für die Protease, aufgrund unzureichender Denaturierung der Sekundärstruktur bzw. der Aggregation hydrophober Abschnitte im Rahmen der Probenaufarbeitung.

Die spektroskopische Untersuchung einzelner Moleküle in kondensierter Phase erstreckt sich erst über einen Zeitraum von zehn Jahren. In dieser verhältnismäßig kurzen Zeit vollzog sich eine rasante Entwicklung mit einer Vielzahl von Ergebnissen. Dies findet seinen Ausdruck in eigenen Tagungen und Zeitschriften und nicht zuletzt auch in einer Nobelkonferenz im Jahre 1999. Während sich anfangs die Untersuchungen auf eine Reihe faszinierender Tieftemperaturexperimente mit spektraler Selektion der einzelnen Moleküle beschränkten, verschob sich seit Mitte der 90er Jahre der Schwerpunkt der Forschung auf diesem Gebiet hin zu Experimenten mit räumlicher Selektion bei Raumtemperatur, die seit kurzer Zeit auch relativ uneingeschränkt bei Tieftemperatur möglich sind. Diese Entwicklung spiegelt sich auch in dieser Dissertation wider. Zu Beginn dieser Arbeit stand eine spektral hochauflösende Apparatur zur Einzelmolekülspektroskopie bei kryogenen Temperaturen zur Verfügung. Mit dieser wurden Einzelmoleküluntersuchungen an dem neu synthetisierten Farbstoff Terrylendiimid (TDI) durchgeführt. TDI ist kein reiner Kohlenwasserstoff, wie die bis dahin üblicherweise verwendeten Chromophore, und lässt sich durch seine Seitengruppen an andere Systeme anbinden. Er zeigt neben exzellenten Fluoreszenzeigenschaften die zur spektralen Selektion nötigen schmalen Absorptionslinien. Wegen seiner Struktur lässt sich TDI nicht in einen Kristall einlagern. Mit Polyethylen und Hexadecan wurden jedoch zwei Matrizen gefunden, die es erlauben, Fluoreszenzanregungsspektren von einzelnen Molekülen zu detektieren. In Hexadecan konnte bei Sättigungsuntersuchungen das theoretisch vorhergesagte Verhalten nachgewiesen werden. Dabei wurden Zählraten von fast 500 000 Counts pro Sekunde von einem einzelnen Molekül erreicht. Durch die Aufnahme und Auswertung der Fluoreszenzintensitäts-Autokorrelationsfunktion konnten die Populations- und Depopulationsraten der Triplett-Subniveaus bestimmt werden. Dabei wurde auch spektrale Diffusion der Moleküle beobachtet, die mit Hilfe von Two-Level Systems (TLS) erklärt werden konnte. Mit einem komplexen theoretischen Modell und aufwendigen numerischen Berechnungen konnte die bei 2,5 K auftretende Verteilung von Linienbreiten der beobachteten Moleküle simuliert werden. Damit konnte den beiden Matrizen über die Analyse ihrer TLS-Dichte ein unterschiedlicher Grad an Unordnung zugeordnet werden. In temperaturabhängigen Untersuchungen der Linienform konnte der Unterschied im Ordnungsgrad der Matrizen bestimmt werden. Ferner konnten die Theorie von Hsu und Skinner in der Tieftemperaturnäherung bestätigt werden und ein tieferer Einblick in die auftretende Dynamik gewonnen werden. In der Auswertung der temperaturabhängigen Linienverschiebung wurde erstmals der Einfluss von Matrixexpansion berücksichtigt und als unverzichtbar für eine gute Beschreibung des Systems erkannt. Parallel zu den ersten Experimenten wurde eine aktive Stabilisierung des Farbsto?asers aufgebaut. Damit konnte eine Verfälschung der Ergebnisse durch Laserdrift ausgeschlossen werden. Weitere Tieftemperaturuntersuchungen hatten die Beobachtung von Förster Energietransfer (oder FRET, Fluorescence Resonance Energy Transfer) an einem individuellen Donor-Akzeptor-Paar in seiner speziellen Konformation zum Ziel. Als Farbstoffmolekül stand ein Bichromophor aus Perylen und kovalent angebundenem TDI zur Verfügung. Obwohl beide Chromophore sich für Einzelmoleküluntersuchungen eignen und inzwischen schon mehrfach verwendet wurden, gelang es nicht, ein bezüglich Linienbreite und Frequenzposition identisches Fluoreszenzanregungsspektrum sowohl über Perylen-Fluoreszenz als auch über TDI-Fluoreszenz (nach Energietransfer) zu detektieren. Der Energietransferprozess scheint mit einem Linienverbreiterungsmechanismus verknüpft zu sein, so dass eine Beobachtung mit dem Aufbau in der Anfangsphase der Dissertation nicht möglich war. Eine Wiederaufnahme dieser Untersuchungen mit der neuen Apparatur ist zukünftigen Doktoranden vorbehalten. Um allgemein temperaturabhängige Untersuchungen an fluoreszierenden Molekülen durchführen zu können, wurde ein Tieftemperaturmikroskop aufgebaut. Dafür wurde die Rastertechnik gewählt. Um die bekannten Probleme des Probenscannens im Kryostaten, wie kleiner Scanbereich und fehlender Zugang im abgekühlten Zustand, zu vermeiden, wurde ein konfokales Laserscanning-Mikroskop entworfen und aufgebaut. Zur Strahlablenkung wurden zwei Galvanometerspiegel gewählt und der Drehpunkt über ein telezentrisches System in das Objektiv abgebildet, das gemeinsam mit der Probe im Kryostaten sitzt. Die Detektion des Fluoreszenzlichts wird von einer hochempfindlichen Avalanche-Photodiode mit geringer Dunkelzählrate übernommen. Die Funktion des Scanners und des gesamten optischen Aufbaus konnte an Testmustern und Testproben erfolgreich demonstriert werden. Einschränkend muss jedoch erwähnt werden, dass die erreichte DetektionseŽzienz die Erwartungen nicht erfüllte. Das liegt im Wesentlichen am Objektiv, aber auch an den Abbildungsfehlern und Reflexionen der zahlreichen Elemente im Strahlengang. Die maximal erreichten Zählraten lagen bei 50 000 Counts pro Sekunde am System Terrylen in Polyethylen. Für Systeme mit einer ausreichend hohen Fluoreszenzrate ist es mit dieser Apparatur möglich, Fluoreszenzbilder, Zeitspuren, spektral hochauflösende Fluoreszenzanregungsspektren, Fluoreszenzspektren und Fluoreszenzkorrelationsfunktionen von einzelnen Molekülen aufzunehmen, um damit spektrale und dynamische Eigenschaften der Moleküle zu bestimmen. Durch Variation der Temperatur können die Temperaturabhängigkeit der Messgrößen und Barrierenhöhen ermittelt werden. Mit der neuen Apparatur wurden Untersuchungen in zwei neuen Themenbereichenbegonnen, nämlich an einzelnen Sondenmolekülen in Nanoporen und an den fluoreszierenden Proteinen GFP (Grün Fluoreszierendes Protein) und PEC (Phycoerythrocyanin). Erste Fluoreszenzanregungsspektren einzelner Terrylen-Moleküle in den Kanalstrukturen von mesoporösen Systemen der M41S-Klasse konnten beobachtet werden. Dabei ist die hohe spektrale Auflösung von großem Vorteil bei der Untersuchung der spektralen Dynamik der Sondenmoleküle. Im Bereich biologischer Proben konnten einzelne Moleküle des Grün Fluoreszierenden Proteins isoliert beobachtet werden. Die Anzahl an Fluoreszenzphotonen pro Molekül, die vor dem ¨Ubergang in einen Dunkelzustand an diesem System detektiert werden konnten, war allerdings sehr gering. Deshalb wurden Untersuchungen an einzelnen Proteinen aus dem Lichtsammelkomplex von Cyanobakterien begonnen, die in einer laufenden Doktorarbeit von P. Zehetmayer fortgeführt werden. Bei den Proteinproben handelt sich um Untereinheiten von Phycoerythrocyanin: die ‹-Untereinheit und das Trimer bzw. Monomer, in denen offenkettige Tetrapyrrhol-Moleküle als Farbstoffe an die Proteinmatrix angebunden sind. Neben Fluoreszenzbildern und Zeitspuren konnten bereits Anregungsspektren detektiert werden, die starke spektrale Dynamik zeigen und weitere Untersuchungen herausfordern. Wesentliche Teile dieser Arbeit wurden bereits in internationalen Zeitschriften und auf Tagungen veröffentlicht. Eine Übersicht befindet sich am Ende unter Veröffentlichungen und Tagungsbeiträge.

Sat, 1 Jan 1994 12:00:00 +0100 http://epub.ub.uni-muenchen.de/2451/ http://epub.ub.uni-muenchen.de/2451/1/2451.pdf Gottschalk, Lothar; Lottspeich, Fritz; Scheer, Hugo Gottschalk, Lothar; Lottspeich, Fritz und Scheer, Hugo (1994): Reconstitution of an allophycocyanin trimer complex containing the C-terminal 21-23 kDa domain of the core-membrane linker polypeptide Lcm. In: Zeitschrift für Naturforschung C: Journal of Biosciences, Vol