Podcasts about NG2

American presidents often use their first meeting with Congress as a chance to lay out their agenda for the next four years. Donald Trump, by contrast, treated last night's address like a campaign rally. Meet Ecuador's tenacious anti-corruption champion (12:18). And co-host Jason Palmer plays an AI bot of Magnus Carlsen at chess (21:39).Jason Palmer v AI Magnus Carlsen: 1. e4 e5 2. Nf3 Nc6 3. d4 exd4 4. c3 Qe7 5. Nxd4 d5 6. Bb5 Qxe4+ 7. Ne2 Qxg2 8. Rg1 Qe4 9. Nd2 Qe7 10. b3 Nf6 11. Ba3 Qxa3 12. Nb1 Qe7 13. Qd4 Bf5 14. Rg5 g6 15. Rg3 Bh6 16. Re3 Bxe3 17. Fxe3 18. Qf4 Ng4 19. h3 Nxe3 20. a4 Ng2+ 21. Kf2. Nxf4 22. Nxf4 Qh4+ 23. Kg2 Qxf4 24. Na3 Be4+ 25. Kg1 Qg3+ 26. Kf1 Qf3+ 27. Ke1 Qxc3+ 28. Kd1 Qxa1+ 29. Kd2 Qxa3 30. Ke3 Qxb3+ 31. Kf4 Qf3+ 32.Kg5 f6+ 33. Kh6 Qxh3# Hosted on Acast. See acast.com/privacy for more information.

American presidents often use their first meeting with Congress as a chance to lay out their agenda for the next four years. Donald Trump, by contrast, treated last night's address like a campaign rally. Meet Ecuador's tenacious anti-corruption champion (12:18). And co-host Jason Palmer plays an AI bot of Magnus Carlsen at chess (21:39).Jason Palmer v AI Magnus Carlsen: 1. e4 e5 2. Nf3 Nc6 3. d4 exd4 4. c3 Qe7 5. Nxd4 d5 6. Bb5 Qxe4+ 7. Ne2 Qxg2 8. Rg1 Qe4 9. Nd2 Qe7 10. b3 Nf6 11. Ba3 Qxa3 12. Nb1 Qe7 13. Qd4 Bf5 14. Rg5 g6 15. Rg3 Bh6 16. Re3 Bxe3 17. Fxe3 18. Qf4 Ng4 19. h3 Nxe3 20. a4 Ng2+ 21. Kf2. Nxf4 22. Nxf4 Qh4+ 23. Kg2 Qxf4 24. Na3 Be4+ 25. Kg1 Qg3+ 26. Kf1 Qf3+ 27. Ke1 Qxc3+ 28. Kd1 Qxa1+ 29. Kd2 Qxa3 30. Ke3 Qxb3+ 31. Kf4 Qf3+ 32.Kg5 f6+ 33. Kh6 Qxh3#

Entrevistamos a Jota Ruiz quien llega celebrando su faceta como cantante de salsa. Hablamos sobre sus experiencias como músico, trabajar para Luis Fonsi y dirigir la orquesta NG2, la union de exponentes de la nueva generación salsera y si se considera parte de la nueva generación salsera y cuanto le ayuda la experiencia que tiene como músico en esta nueva etapa. Vacilen No Puedo Tengo Ensayo TV Facebook: https://www.facebook.com/nopuedotengoensayotv Instagram: https://www.instagram.com/nptetv/ Yadiel Carrasco Instagram: https://instagram.com/yadielcarrasco?igshid=18pwybdws2ztp F07 Media Instagram: https://www.instagram.com/f07media/ Bryan Gómez Instagram: https://instagram.com/bryanphotography96?igshid=1j9bmit6s7dy4 Karla Pérez Instagram: https://www.instagram.com/karlandrea25/ "Las expresiones vertidas en No Puedo Tengo Ensayo TV, no representan, ni la opinión ni la postura, ni la política institucional de Yadiel Carrasco y/o la producción de No puedo Tengo ensayo TV." --- Support this podcast: https://podcasters.spotify.com/pod/show/nopuedotengoensayotv/support

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2023.07.26.550616v1?rss=1 Authors: Galichet, C., Rizzoti, K., Lovell-Badge, R. Abstract: The median eminence (ME), located at the base of the hypothalamus, is an essential centre of information exchange between the brain and the pituitary. We and others previously showed that mutations and duplications affecting the transcription factor SOX3/Sox3 result in hypopituitarism, and this is likely of hypothalamic origin. We demonstrate here that the absence of Sox3 predominantly affects the ME with phenotypes that first occur in juvenile animals, despite the embryonic onset of SOX3 expression. In the pituitary, reduction in hormone levels correlates with a lack of endocrine cell maturation. In parallel, ME NG2-glia renewal and oligodendrocytic differentiation potential are affected. We further show that low-dose aspirin treatment, which is known to affect NG2-glia, or changes in gut microbiota, rescue both proliferative defects and hypopituitarism in Sox3 mutants. Our study highlights a central role of NG2-glia for ME function during a transitional period of post-natal development, and indicates their sensitivity to environmental signals. Copy rights belong to original authors. Visit the link for more info Podcast created by Paper Player, LLC

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2023.04.17.537129v1?rss=1 Authors: Hardt, R., Dehghani, A., Schoor, C., Goedderz, M., Cengiz Winter, N., Ahmadi, S., Sharma, R., Schork, K., Eisenacher, M., Gieselmann, V., Winter, D. Abstract: Oligodendrocytes are generated via a two-step mechanism from pluripotent neural stem cells (NSCs): after differentiation of NSCs to oligodendrocyte precursor/NG2 cells (OPCs), they further develop into mature oligodendrocytes. The first step of this differentiation process is only incompletely understood. In this study, we utilized the neurosphere assay to investigate NSC to OPC differentiation in a time course-dependent manner by mass spectrometry-based (phospho-) proteomics. We identify double cortin like kinase 1 (Dclk1) as one of the most prominently regulated proteins in both datasets, and show that it undergoes a gradual transition between its short/long isoform during NSC to OPC differentiation. This is regulated by phosphorylation of its SP-rich region, resulting in inhibition of proteolytic Dclk1 long cleavage, and therefore Dclk1 short generation. Through interactome analyses of different Dclk1 isoforms by proximity biotinylation, we characterize their individual putative interaction partners and substrates. Copy rights belong to original authors. Visit the link for more info Podcast created by Paper Player, LLC

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2023.04.04.535590v1?rss=1 Authors: Liu, Y., Guo, J., Matoga, M., Korotkova, M., Jakobsson, P.-J., Aguzzi, A. Abstract: Oligodendrocyte-lineage cells of patients and animal models undergo prominent changes in various neurodegenerative disorders. This raises the question of how myelinating cells interact with neurodegenerative processes. Here, we investigated the role of oligodendrocyte precursor cells (NG2 glia) in prion infections. We found that NG2 glia were activated in prion-infected cerebellar organotypic cultured slices (COCS) and in brains of prion-inoculated mice. In both model systems, depletion of NG2 glia exacerbated prion-induced neurodegeneration and accelerated prion pathology, suggesting a protective effect for NG2 glia. Loss of NG2 glia unleashed a microglial reaction promoting the biosynthesis of prostaglandin E2 (PGE2), which augmented prion toxicity in HovS cells and COCS through binding to the EP1 and EP4 receptors. Pharmacological or genetic inhibition of PGE2 biosynthesis attenuated prion-induced neurodegeneration in COCS and mice, antagonized the enhanced neurodegeneration in NG2 glia-depleted COCS and brains after prion infection, and dampened the acceleration of prion disease in NG2 glia-depleted mice. These data unveil a non-cell-autonomous interaction involving NG2 glia and microglia in prion disease and suggest that PGE2 signaling may represent an actionable target against prion diseases. Copy rights belong to original authors. Visit the link for more info Podcast created by Paper Player, LLC

Si eres amante de la salsa este podcast es para ti. Hablamos con el ex integrante de NG2 y el creador de Sesiones desde la Loma el gran Norberto Vélez. Norbert nos hablo sobre el proceso de creación del programa sesiones desde la Loma, su programa con Tito Rojas y cuanto le afectó su partida, su momento mas bajo luego de salir del duo NG2 y como logró sobrellevarlo y nos da detalles sobre una nueva producción musical

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2023.02.20.529180v1?rss=1 Authors: Janeckova, L., Knotek, T., Kriska, J., Hermanova, Z., Kirdajova, D., Kubovciak, J., Berkova, L., Tureckova, J., Camacho Garcia, S., Galuskova, K., Kolar, M., Anderova, M., Korinek, V. Abstract: Glia cells expressing neuron-glial antigen 2 (NG2) play a critical role as oligodendrocyte precursor cells (OPCs) in the healthy brain; however, their differentiation potential after ischemic injury remains an unresolved question. Here, we aimed to elucidate the heterogeneity and role of NG2 glia in the ischemic brain. We used transgenic mice to label NG2-expressing cells and their progeny with red fluorescent protein tdTomato in the healthy brains and those after focal cerebral ischemia (FCI). Based on single-cell RNA sequencing, the labeled glial cells were divided into five distinct subpopulations. The identity of these subpopulations was determined based on gene expression patterns. In addition, membrane properties were further analyzed using the patch-clamp technique. Three of the observed subpopulations represented OPCs, whereas the fourth group exhibited characteristics of cells destined for oligodendrocyte fate. The fifth subpopulation of NG2 glia carried astrocytic markers. Importantly, we detected features of neural progenitors in these cells. This subpopulation was present in both healthy and post-ischemic tissue; however, its gene expression changed after ischemia, with genes related to neurogenesis being more abundant. Neurogenic gene expression was monitored over time and complemented by immunohistochemical staining, which showed increased numbers of Purkinje cell protein 4-positive NG2 cells at the edge of the ischemic lesion 12 days after FCI, and NeuN-positive NG2 cells 28 days after injury, indicating the existence of neuron-like cells that develop from NG2 glia in the ischemic tissue. Our results provide further insight into the differentiation plasticity and neurogenic potential of NG2 glia after stroke. Copy rights belong to original authors. Visit the link for more info Podcast created by Paper Player, LLC

Today's the last day to get your SuperDerek Hoodie at shop.superderekrpgs.com/ Snag your Physical copy of Eiyuden Chronicle Rising Here to support the show: ✩ Audio Version ✩ ► https://youtu.be/iILNtFqerGISOCIAL LINKS --------------------------------------------------- ✩ Derek ✩ ►Twitter: https://twitter.com/SuperDerekRPGs ►Twitch: https://www.twitch.tv/SuperDerekRPGs ►Discord: https://discord.me/superderek✩ Baku ✩ ►Twitter: https://www.twitter.com/BakusanJRPG ►Twitch: https://www.twitch.tv/BakusanJRPG►Youtube: https://www.youtube.com/weebsauce ►Discord: https://discord.me/ABCUPCOMING RELEASES --------------------------------------------------- ►Neptunia: Sisters vs. Sisters 01/24/2023https://www.youtube.com/watch?v=aSjeDksK6nw ►Eiyuden Chronicle: Rising (Physical) 01/26/2023Switch: https://www.redartgames.com/accueil/402-eiyuden-chronicle-rising-switch-pre-order.html?rag=9 PS4: https://www.redartgames.com/accueil/400-eiyuden-chronicle-rising-ps4-pre-order.html?rag=9 PS5: https://www.redartgames.com/accueil/401-eiyuden-chronicle-rising-ps4-pre-order.html?rag=9 ►Alice Escaped! 01/27/2023https://www.youtube.com/watch?v=Yzt7pb_ryV8 ►Forespoken 01/24/2023https://www.youtube.com/watch?v=7-5Fa5meEo4 GAME ANNOUNCEMENTS --------------------------------------------------- ►LIttle Witch Nobeta (Physical) 03/07/2023https://iffysonlinestore.com/little-witch-nobeta-LE/ ►Brave's Rage 02/02/2023https://www.youtube.com/watch?v=3UzrAhLnwvI ►White Day 2: The Flower That Tells Lieshttps://www.youtube.com/watch?v=Vg6cf6L4itU ►Dokapon Kingdom Connect Spring 2023https://www.youtube.com/watch?v=cfU6eDroSN4 ►Granblue Fantasy Versus Rising 2023https://www.youtube.com/watch?v=4Ln58g217C4 ►Granblue Fantasy Relink 2023https://www.youtube.com/watch?v=JWmQZEBC9oU EXTERNAL LINKS --------------------------------------------------- ►Atelier Ryza 3 Delayedhttps://twitter.com/GustAtelierPR/status/1615559792973737984 NieR Automata Anime Delayedhttps://nierautomata-anime.com/news/?id=62357 ►Tales of Symphonia Anime on YouTubehttps://www.youtube.com/@Tales_of/videos ►Sonic Prime Season 2 Is Coming Later This Year https://twitter.com/sonic_hedgehog/status/1615767982214631425 ►Eiyuden Chronicle: Hundred Heroes DLC 3 Votehttps://twitter.com/eiyuden/status/1616246691153301504 ►Monster Menu: The Scavenger's Cookbook Reverse Cover Votehttps://nisamerica.com/monster-menu/contest/reverse ►Vox Machina now on Amazon Primehttps://www.amazon.com/Legend-Vox-Machina-Season/dp/B09PZG2979 ►Sony's definition of a JRPGhttps://www.playstation.com/en-us/editorial/this-month-on-playstation/great-japanese-rpgs-on-ps4/ ►Legend of Mana Serafina Nendoroidhttps://www.goodsmileus.com/product/nendoroid-serafina-9969 ►Trailers of Cold Steel II Altina Orion Figure (1/7 scale)https://twitter.com/quesq_info/status/1615982393306873857/ TIMESTAMPS--------------------------------------------------- Welcome Back to Hit Point!0:00:00 Welcome Back to Hit Point!0:01:10 Intro0:01:44 Baku how are you doing?0:05:06 Derek what are you up to? Last Week's Comments:0:14:30 Comment 1: Do you guys know if there are any places in the U.S. where you can pre-order the Renne Bright figure?0:16:22 Comment 2: New Patch for Trails from Zero0:17:48 Comment 3: Baku talking about wanting more Estelle when I'm a massive Kevin fan...0:21:00 Voice Mail: What are some classic Computer RPGs you got in?Game Delays0:31:34 Atlier Ryza 3 delayed Updates & DLC Round-Up0:33:58 YS XIII Steam(PC?) Update0:35:43 Sword Art Online: Alicization Lycoris - New DLC0:37:24 Monter Hunter Rise Sunbreak Expansion Upcoming Releases This Week 0:40:27 Neptunia: Sisters VS Sisters0:46:28 Alice Escaped! 0:49:31 Forspoken New Game Announced:1:03:23 Little Witch Nobeta PS4 and Switch physical release1:05:30 Brave's Rage1:07:34 Sword Art Online: Last Recollection1:12:08 White Day 2: The Flower that tell Lies1:16:19 Dokapon Kingdom: Connect1:22:20 Granblue Fantasy: Versus Rising1:25:46 Granblue Fantasy: RelinkIndustry News:1:30:45 Nier Automata's Anime has been indefinitely delayed1:32:28 Tales of Symphonia on YouTube1:33:18 Second Season for Sonic Prime1:33:58 Eiyuden Chronicle: Hundred Heroes DLC 3 Vote1:35:00 Reverse Cover Vote for Monster Menu: The Scavenger's Cookbook1:36:13 The Legend of Vox Machina On Prime1:38:58 Sony what is an JRPG? Merch News:1:56:15 Legend of Mana: Nendoroid Serafina2:00:18 Trails of Cold Steel 2: Altina Orion Black Rabbit Special Duty Suit Industry News 2: Electric Boogaloo:2:02:51 Why did Sony make the Webpage?Responding to Super Chats & Outro 2:10:14 Super Chat 1: Happy Birtday to yumyumrazon2:10:42 Super Chat 2: Jack Garland would make Forspoken more "FUN"2:11:10 Super Chat 3: Congrats to everyone who secured they SuperDerek Merch2:12:15 Super Chat 4: Bayo, DMC or NG2:15:01 Outro

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2022.09.28.509944v1?rss=1 Authors: Kim, H., Skuba, A., Xia, J., Han, S. B., Zhai, J., Yoo, R., Hu, H., Kang, S. H., Son, Y.-J. Abstract: Adult mammalian spinal cord stops re-entering sensory axons, resulting in incurable sensory loss after dorsal root injury. Challenging the prevailing view, we previously showed that axons rapidly stop by forming synaptic, not dystrophic endings, and that myelin inhibitors and CSPGs are not the primary mechanism arresting axons. Here, we report that in adult mice dystrophic endings are formed by occasional axons embedded in fibrous collagens, exclusively at the CNS:PNS boundary where oligodendrocyte precursor cells (OPCs, or NG2 glia) are sparse. Most axons stop beyond the boundary in extensive contacts with OPC processes. Live imaging, immuno-EM and OPC-DRG co-culture show that axonal synapses form on OPCs. Genetic OPC ablation, although incomplete, enables many axons to continue regenerating deep into the spinal cord. These data, together with earlier findings, conclusively demonstrate that axons stop largely because OPCs stimulate presynaptic differentiation. We identify OPCs as a major regenerative barrier at the CNS:PNS border. Copy rights belong to original authors. Visit the link for more info Podcast created by PaperPlayer

Steve Cooper's reds went into another busy week and looked to make home advantage count as they took on Sheffield United and Preston North End in NG2. Along the way we'd like to feature other fans views and opinions so if you want to contact us you can find us on Email: timeaddedonpod@outlook.com Twitter: @timeaddedonpod Music by Ryan Andersen - Never Sleep (2019)

The reds return from the international break wanting to carry on their impressive momentum as they faced Blackpool at home this weekend. We also look ahead to a return to busy league action as we return to our travels to face Bristol City in midweek as well as returning to NG2 on Sunday to face Fulham. Along the way we'd like to feature other fans views and opinions so if you want to contact us you can find us on Email: timeaddedonpod@outlook.com  Twitter: @timeaddedonpod   Music by Ryan Andersen - Never Sleep (2019)

We analyse a week where Forest faced relegation battlers Rotherham and our friends up the A52 Wayne Rooney's Derby - certainly not Barcelona. We also preview the upcoming week which sees Forest taking on Luton at NG2 and taking a trip to Vicarage Road to face promotion contenders Watford.  Along the way we'd like to feature other fans views and opinions so if you want to contact us you can find us on Email: timeaddedonpod@outlook.com  Twitter: @timeaddedonpod        Music by Ryan Andersen - Never Sleep (2019)

Tito started his career as a Salsa Dancer by his parent's initiative, both musicians.  A top level dancer of many genres such as: Jazz, Merengue, "Bomba y Plena", Rumba, Modern Dance and Tap among others. He has visited more than 100 cities worldwide giving performances and classes, with his wife and dance partner Tamara Livolsi.Having participated in various television commercials, dance videos and movies such as, "Dirty Dancing: Havana Nights", Yellow, El Cantante: Hector Lavoe and American Gangster as dancers, he is no stranger to the stage.Today, Tito is not only a Salsa Dancer, he also is the Director of the San Juan City Salsa Dance Program and has made the choreographic works for concerts of artists such as: Gilberto Santa Rosa, Víctor Manuelle, El Gran Combo, Michael Stuart, Tito Nieves, Rubén Blades, Richie Rey y Bobby Cruz, Domingo Quiñones, Ismael Miranda, Elvis Crespo, Cheo Feliciano, Modesto Cepeda, Andy Montañez, NG2, Luis "Perico" Ortiz, Tommy Olivencia, Julio Voltio and Tego Calderón. He was also the choreographer for the "Bomba" and "Cha-Cha-Cha" routine in Ricky Martin's appearance in Miss Universe 2001 and the Celia Cruz "Azúcar" Special in Miami in 2003. Even after all this stardom, Tito remains humble, kind and readily shares his knowledge with so many!Don't forget to subscribe and leave a review!

¡ #HOY en #APOYALONUESTRO !@cynthia_mmartinez nos habla de su libro #NoTePasoPorPend el cual sirve para motivar a los lectores hacer sus metas realidad.@gerardorivasoficial nos dice que para esta navidad es #ADerretirElHielo y como fue ser parte del dúo @ng2 @yeikamusicapr nos trae la navidad con su sencillo #ESOESNAVIDAD Esto y más a las 12PM por radioactivapr.com junto a @cameraandaction

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2020.10.27.357244v1?rss=1 Authors: Zhou, H., He, Y., Yang, Y., Wang, Z., Wang, Q., Hu, C., Wang, X., Lu, S., Li, K., Luan, Z. Abstract: NG2 and A2B5 are important biological markers of human oligodendrocyte progenitor cells. To study their functional differences during the development of human oligodendrocyte progenitor cells to oligodendrocytes, we used cell sorting technology and obtained a large number of sterile, high-purity NG2+/- and A2B5+/- cells with high viability. Further research was then conducted via in vitro cell proliferation and migration assays, single-cell sequencing, mRNA sequencing, and cell transplantation into shiverer mice. The results showed that the migration ability of the cells was inversely proportional to the myelination ability. NG2 may be a marker of early oligodendrocyte progenitor cells and is conducive to cell migration and proliferation, while A2B5 may be a marker of slightly mature oligodendrocyte progenitor cells and is conducive to cell differentiation. Further, cell migration, proliferation, and myelination capacity of the negative cell population were stronger than those of the positive cell population. In summary, these results suggest that oligodendrocyte progenitor cells in the mid-stage may be more suitable for clinical cell transplantation to treat demyelinating diseases. Copy rights belong to original authors. Visit the link for more info

❌ Un artista que hemos visto su desarrollo desde niño con los Rocolos, como percusionista de grandes orquestas y por 16 años juntos al dúo de voces NG2. Ahora su carrera tomo otro rumbo... La Salsa de Hoy entrevista a Gerardo Rivas!

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2020.09.23.309682v1?rss=1 Authors: Chacon-de-la-Rocha, I., Fryatt, G., Rivera, A., Restani, L., Caleo, M., Raineteau, O., Gomez-Nicola, D., Butt, A. M. Abstract: Oligodendrocyte progenitor cells (OPCs) are responsible for generating oligodendrocytes, the myelinating cells of the CNS. Life-long myelination is promoted by neuronal activity and is essential for neural network plasticity and learning. OPCs are known to contact synapses and it is proposed that neuronal synaptic activity in turn regulates OPC proliferation and differentiation. To examine this in the adult, we performed unilateral injection of the synaptic blocker botulinum neurotoxin A (BoNT/A) into the hippocampus of adult mice. We confirm BoNT/A cleaves SNAP-25 in the CA1 are of the hippocampus, which has been proven to block neurotransmission. Notably, synaptic silencing by BoNT/A significantly decreased OPC density and caused their shrinkage, as determined by immunolabelling for the OPC marker NG2. Inhibition of synaptic activity resulted in an overall decrease in the number of OPC processes, as well as a decrease in their lengths and branching frequency. These data indicate that synaptic activity is important for maintaining adult OPC numbers and cellular integrity, which is relevant to pathological scenarios characterized by decreased synaptic activity. Copy rights belong to original authors. Visit the link for more info

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2020.09.23.309666v1?rss=1 Authors: Chacon-de-la-Rocha, I., Fryatt, G., Rivera, A. D., Verkhratsky, A., Raineteau, O., Gomez-Nicola, D. M., Butt, A. M. Abstract: Myelin disruption is a feature of natural aging and of Alzheimer's disease (AD). In the CNS, myelin is produced by oligodendrocytes, which are generated throughout life by oligodendrocyte progenitor cells (OPCs). Here, we examined age-related changes in OPCs in APP/PS1 mice, a model for AD-like pathology, compared with non-transgenic (Tg) age-matched controls. Analysis was performed in the CA1 area of the hippocampus following immunolabelling for NG2 with the nuclear dye Hoescht, to identify OPC and OPC sister cells, a measure of OPC replication, together with Gpr17 and Olig2 for oligodendrocytes and myelin basic protein (MBP) immunostaining as a measure of myelination. The results indicate a decrease in the number of OPCs between 9 and 14 months in natural ageing and this occurred earlier at 9 months in APP/PS1 mice, without further decline at 14 months. The number of OPC sister cells was unaltered in natural aging, but declined significantly at 14-months in APP/PS1 mice. The number of GPR17+ and Olig2+ oligodendrocytes was not altered in APP/PS1, whereas MBP immunostaining increased between 9 and 14 months in natural ageing, but not in APP/PS1 mice. Notably, OPCs displayed marked morphological changes at 14 months in APP/PS1 mice, characterized by an overall shrinkage of OPC process domains and increased process branching, characteristic of reactive pathological changes. The results indicate that OPC and myelin disruption are pathological signs in the APP/PS1 mouse model of AD. Copy rights belong to original authors. Visit the link for more info

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2020.08.19.257113v1?rss=1 Authors: Treccani, G., Yigit, H., Lingner, T., Schleussner, V., Wennstrom, M., Herzog, D. P., Fricke, M., Wegener, G., Mittmann, T., Trotter, J., Muller, M. Abstract: Background: The detrimental effects of early adversity on brain development and adult mental health are well established. While studies have identified myelination processes and oligodendrocytes as targets of early adversity across species, knowledge about the precise molecular mechanisms and cell populations involved is still lacking. NG2+ cells are oligodendrocyte precursor cells with unique properties, as they form synapses with neurons, engage in cell-cell communication and respond to stress hormones. Methods: Using an established mouse model of early life stress (ELS) we performed cell-type specific molecular profiling in hippocampal NG2 cells at early postnatal and adult stages. To further dissect the impact of glucocorticoids on ELS-induced transcriptional changes, we integrated our data with available Chip-seq data on genomic binding sites of the glucocorticoid receptor (GR). The functional relevance of one candidate gene, Scn7a, was confirmed by electrophysiological recordings in hippocampal NG2 cells. Results: ELS specifically targeted the hippocampal NG2 cell transcriptome, and the molecular changes correlated with the ELS-induced increase of corticosterone. The overlap analysis with Chip-seq data on genomic binding sites of the GR revealed nine overlapping genes. Among those Scn7a, coding for a subunit of sodium channels, remained upregulated until adulthood in ELS animals. Upregulation of Scn7a was accompanied by an increase in the density of voltage-gated sodium channel activated currents in hippocampal NG2 cells. Conclusions: Our findings indicate that ELS specifically targets the transcriptional profiles and electrophysiological properties of hippocampal NG2 glia. Considering that voltage-gated sodium channels are important for the NG2 cell-to-neuron communication, our findings may provide novel insights into the pathophysiological processes underlying stress related mental disorders. Copy rights belong to original authors. Visit the link for more info

en este episodio hablamos con Gerardo Rivas ex integrante de NG2 e hijo de Jerry Rivas cantante del Gran Combo de Puerto Rico

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2020.05.29.124586v1?rss=1 Authors: Ivanova, E., Corona, C., Eleftheriou, C. G., Bianchimano, P., Sagdullaev, B. T. Abstract: Pericytes are a unique class of mural cells essential for angiogenesis, maintenance of the vasculature and are key players in microvascular pathology. However, their diversity and specific roles are poorly understood, limiting our insight into vascular physiology and the ability to develop effective therapies. Here, in the mouse retina, a tractable model of the CNS, we evaluated distinct classes of mural cells along the vascular tree for both structural characterization and physiological manipulation of blood flow. To accomplish this, we first tested three inducible mural cell-specific mouse lines using a sensitive Ai14 reporter and tamoxifen application either by a systemic injection, or by local administration in the form of eye drops. Across three lines, under either the PDGFRb or NG2 promoter, the specificity and pattern of Cre activation varied significantly. In particular, a mouse line with Cre under the NG2 promoter resulted in sparse TdTomato labeling of mural cells, allowing for an unambiguous characterization of anatomical features of individual sphincter cells and capillary pericytes. Furthermore, in one PDGFRb line, we found that focal eye drop application of tamoxifen led to an exclusive Cre-activation in pericytes, without affecting arterial mural cells. We then used this approach to boost capillary blood flow by selective expression of Halorhodopsin, a highly precise hyperpolarizing optogenetic actuator. The ability to exclusively target capillary pericytes may prove a precise and potentially powerful tool to treat microcirculation deficit, a common pathology in numerous diseases. Copy rights belong to original authors. Visit the link for more info

Reacción personal a la pausa que tomarán Norberto Vélez y Gerardo Rivas con su proyecto musical NG2.

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A brief preview of the upcoming full episode. Host Kevin Patton reveals upcoming topics (therapy to grow brain cells, smelling without olfactory bulbs, flashcards on steroids) Plus word dissections and a book club recommendation (The Body —A Guide for Occupants), and more! 00:19 | Topics 02:02 | Sponsored by HAPI Online Graduate Program 02:43 | Word Dissection 13:45 | Sponsored by HAPS 14:21 | Book Club 19:06 | Sponsored by AAA 19:44 | Staying Connected If you cannot see or activate the audio player click here. Questions & Feedback: 1-833-LION-DEN (1-833-546-6336) Follow The A&P Professor on Twitter, Facebook, Blogger, Nuzzel, Tumblr, or Instagram! Upcoming Topics 2 minutes Something Smells Odd People who can smell just fine but whose MRIs show that they don't have olfactory bulbs Revisiting the topic of making new neurons in the adult human brain. Gene therapy for repairing brain injury Flashcards Their hidden powers Beyond the basics Sponsored by HAPI Online Graduate Program 0.5 minute The Master of Science in Human Anatomy & Physiology Instruction—the MS-HAPI—is a graduate program for A&P teachers. A combination of science courses (enough to qualify you to teach at the college level) and courses in contemporary instructional practice, this program helps you power up  your teaching. Kevin Patton is a faculty member in this program. Check it out! nycc.edu/hapi Word Dissection 11 minutes anosmia cribriform plate ethmoid bone polydendrocyte oligodendrocyte NG2 cell (NG2 glia) flashcard hornbook (see image my-ap.us/2YB9MMO) Sponsored by HAPS 0.5 minute The Human Anatomy & Physiology Society (HAPS) is a sponsor of this podcast.  You can help appreciate their support by clicking the link below and checking out the many resources and benefits found there. Don't forget the HAPS Awards, which provide assistance for participating in the HAPS Annual Conference. Anatomy & Physiology Society theAPprofessor.org/haps Book Club 4.5 minutes The Body – A Guide for Occupants by Bill Bryson amzn.to/2rBQNWm Recommended by Marty Port Storytelling is the Heart of Teaching A&P | Episode 12 Anatomic Variations in Humans | Fabella | Situs Inversus | Episode 43 Special opportunity Contribute YOUR book recommendation for A&P teachers! Be sure include your reasons for recommending it Any contribution used will receive a $25 gift certificate The best contribution is one that you have recorded in your own voice (or in a voicemail at 1-833-LION-DEN) Check out The A&P Professor Book Club Sponsored by AAA 0.5 minutes A searchable transcript for this episode, as well as the captioned audiogram of this episode, are sponsored by the American Association for Anatomy (AAA) at anatomy.org. Searchable transcript Captioned audiogram    If the hyperlinks here are not active, go to TAPPradio.org to find the episode page. More details at the episode page. Transcript available at the script page. Listen to any episode on your Alexa device. Need help accessing resources locked behind a paywall? Check out this advice from Episode 32 to get what you need! https://youtu.be/JU_l76JGwVw?t=440   Tools & Resources  Amazon TextExpander Rev.com Snagit & Camtasia The A&P Professor Logo Items   Sponsors   Transcript and captions for this episode are supported by the  American Association for Anatomy. anatomy.org   The Human Anatomy & Physiology Society  also provides marketing support for this podcast.  theAPprofessor.org/haps   Distribution of this episode is supported by  NYCC's online graduate program in  Human Anatomy & Physiology Instruction (HAPI)  nycc.edu/hapi   Clicking on sponsor links  helps let them know you appreciate their support of this podcast!   Follow The A&P Professor on  Twitter, Facebook, Blogger, Nuzzel, Tumblr, or Instagram!   The A&P Professor® and Lion Den® are registered trademarks of Lion Den Inc. (Kevin Patton)  

Gerardo Rivas, integrante de NG2, hijo de Jerry Rivas, nos habla hoy de cómo llega al mundo de la música desde pequeño con Gerardito y los Rocolos, sus inicios en los comerciales de arroz rico y de todo lo que aprendió trabajando con Victor Manuelle.

Norberto Vélez del grupo NG2 tiene una conversación muy diferente con Francisco. Habla de sus comienzos en la música, las dificultades luego de María, momentos difíciles en su carrera y crecimiento profesional y más. Te enterarás de datos que Norberto nunca había compartido antes. BUENASSSS.

En este episodio conversamos con Los Oquen2 una orquesta que esta comenzando su carrera desde Puerto Rico. Hablamos de sus inicios, de su influencias del genero urbano, de su colaboracion con NG2. Y hasta nos cuentan como grabaron los "covers" de reggaeton en salsa sin tener el permiso de los artistas urbanos. Presentando su primera produccion a la calle La Promesa. Llegan Los Oquen2. Disfruten de esta entrevista y recuerden compartir el episodio.

1:25 - Introducing Travis Tidwell Form.io Blog Twitter Github 2:35 - What’s a form and why would you build one? 8:30 - Making changes to API-driven forms Swagger 13:50 - Forms and GraphQL 15:10 - Working with conditions 16:55 - Serverless applications 24:20 - Microservices, actions, and web hooks 29:15 - Are all PWA’s serverless? 31:10 - Building apps API-first instead of mobile-first 36:00- The user experience and the API-first approach 38:10 - The inspection example 42:50 - Rendering widgets using Angular https://github.com/formio/ng2-formio 46:50 - Teaching the “why” and the “how” Picks: Tools of Titans by Timothy Ferriss (Lukas) Shai Reznik’s HiRez.io (Alyssa) Shai Reznik’s AiA episodes: 046 - Prepping for NG2 040 - ng-wat Objects of Desire: Design and Society since 1750 by Adrian Forty (Ward) Moleskine notebooks (Charles) Asana (Charles) Westworld (Travis)

1:25 - Introducing Travis Tidwell Form.io Blog Twitter Github 2:35 - What’s a form and why would you build one? 8:30 - Making changes to API-driven forms Swagger 13:50 - Forms and GraphQL 15:10 - Working with conditions 16:55 - Serverless applications 24:20 - Microservices, actions, and web hooks 29:15 - Are all PWA’s serverless? 31:10 - Building apps API-first instead of mobile-first 36:00- The user experience and the API-first approach 38:10 - The inspection example 42:50 - Rendering widgets using Angular https://github.com/formio/ng2-formio 46:50 - Teaching the “why” and the “how” Picks: Tools of Titans by Timothy Ferriss (Lukas) Shai Reznik’s HiRez.io (Alyssa) Shai Reznik’s AiA episodes: 046 - Prepping for NG2 040 - ng-wat Objects of Desire: Design and Society since 1750 by Adrian Forty (Ward) Moleskine notebooks (Charles) Asana (Charles) Westworld (Travis)

1:25 - Introducing Travis Tidwell Form.io Blog Twitter Github 2:35 - What’s a form and why would you build one? 8:30 - Making changes to API-driven forms Swagger 13:50 - Forms and GraphQL 15:10 - Working with conditions 16:55 - Serverless applications 24:20 - Microservices, actions, and web hooks 29:15 - Are all PWA’s serverless? 31:10 - Building apps API-first instead of mobile-first 36:00- The user experience and the API-first approach 38:10 - The inspection example 42:50 - Rendering widgets using Angular https://github.com/formio/ng2-formio 46:50 - Teaching the “why” and the “how” Picks: Tools of Titans by Timothy Ferriss (Lukas) Shai Reznik’s HiRez.io (Alyssa) Shai Reznik’s AiA episodes: 046 - Prepping for NG2 040 - ng-wat Objects of Desire: Design and Society since 1750 by Adrian Forty (Ward) Moleskine notebooks (Charles) Asana (Charles) Westworld (Travis)

En este episodio Gerardo nos cuenta los dificil que fue grabar unos de sus exitos "Como Amigo No" & nos presentan su mas reciente sencillo "Aqui Estoy". Recuerden compartirlo con sus amistades y dejar su comentario en iTunes. #Ng2 #Salsa #Podcast #NacionSalsa

En este epidosio Gerardo nos cuenta quien es su percusionista favorito, de la plaza que los impresionó cuando llevaron su show y de su quimica en tarima con norberto. #NacionSalsa #Salsa #ng2 #PuertoRico #podcast

En este episodio hablamos con Gerardo Rivas de la orquesta NG2 de Puerto Rico. Hijo de Jerry Rivas de El Gran Combo de Puerto Rico. Nos cuenta el comienzo de la orquesta, donde tocaron por primera vez, y nos revela en que profesion NO lo aceptaron. Disfruten de nuestra 1 era parte a la entrevista con NG2!! Recuerden compartirlos en sus redes sociales!! #Salsa #podcast #NacionSalsa #NG2 #Entrevista #

ngAir 77 - Todd Motto- The Man The Myth The Legend Show Notes   Sponsor pitch for Angular training Mention the live show questions on twitter with #ngair Intros What’re you working on etc ngMigrate 1.5 component courses Why components for Angular 1.x? Path to Angular 2 Lifecycle hooks Uni dataflow Coding demo (if needed/time providing) Angular modules Angular 1.x modules Angular @NgModule What does a developer advocate do? (Olivier)   Tips & Picks   Justin Schwartzenberger Rob Wormald helping to explain NgModule for larger applications https://github.com/angular/angular/issues/10552#issuecomment-241556913 Ward Bell explaining providers staying on component metadata https://github.com/angular/angular/issues/10552#issuecomment-241576063 Rob assuring us of Angular 2 API complete status https://github.com/angular/angular/issues/10552#issuecomment-241578111 Austin McDaniel Angular2 HMR w/ Webpack Plugin ( works w/ RC5 ) https://github.com/AngularClass/angular2-hmr-loader Angular Material 1.1 Release & Angular2 Version has 18 components now http://angularjs.blogspot.com/2016/08/angular-material-11-and-2x.html Olivier Combe http://blog.angular-university.io/angular2-ngmodule/ Ng2-translate recommended by Ionic 2 Todd Motto  @ChrisThielen for his ui-router work --- Support this podcast: https://anchor.fm/angularair/support

Edicion 6 esta es pa'l bailador. Musica bailable con Oscar D'Leon en Vivo desde el Copacabana, Lalo Rodriguez con Machito, NG2 y un tema homenaje a Colombia, Republica Dominicana y Puerto Rico y presentamos la nueva orquesta de Colombia Tono Barrio. Que lo disfruten!! Recuerden Play & Share!

02:39 - When is Angular 2 out? Do you feel like other frameworks (React) are becoming more popular and that people are moving over to them? Will people come back to Angular? 21:01 - How can you convince a client to pick Angular over something else like React? 26:19 - Should I continue learning Angular 1 or move on to Angular 2? Will there still be jobs where I can use Angular 1 after Angular 2 is released? 27:38 - To Typescript or not to Typescript? 33:02 - How do you integrate third-party libraries like Firebase or D3 into an NG2 app, taking into account that NG2 runs on one-way dataflow paradigm fueled by an Rx implementation? 36:14 - The Typescript autocomplete/help, etc. that we were discussing before: what tools or IDEs are we talking about? Visual Studio Code 38:26 - What is the current state of the Angular 2 router? 46:08 - Any really good recent D3/Angular resources? What are some things to look at in regards to performance when implementing D3 with Angular? Aysegul Yonet and Patrick Stapleton: Creating d3 components with Angular2 and TypeScript @ ng-vegas 2015 49:40 - How do you structure a really large Angular application? John Papa’s Angular Style Guide Picks Angular 2 (Patrick) Angular Tips (Patrick) Observables and the Angular 2 HTTP library (Rob) Go find yourself a local Hackathon (Ward) Visual Studio Code (John) Rob’s Angular Remote Conf talk (Lukas) The Introduction to Reactive Programming series on Egghead.io (Lukas) Periscope (Chuck) Magnus SnapPod Compact Tabletop Tripod (Chuck) ChargerCity MegaGrab2 Easy-Adjust Smartphone Holder Mount & 360º Swivel Adjustment Selfie Video Recording Camera Tripod Adapter (Chuck)

02:39 - When is Angular 2 out? Do you feel like other frameworks (React) are becoming more popular and that people are moving over to them? Will people come back to Angular? 21:01 - How can you convince a client to pick Angular over something else like React? 26:19 - Should I continue learning Angular 1 or move on to Angular 2? Will there still be jobs where I can use Angular 1 after Angular 2 is released? 27:38 - To Typescript or not to Typescript? 33:02 - How do you integrate third-party libraries like Firebase or D3 into an NG2 app, taking into account that NG2 runs on one-way dataflow paradigm fueled by an Rx implementation? 36:14 - The Typescript autocomplete/help, etc. that we were discussing before: what tools or IDEs are we talking about? Visual Studio Code 38:26 - What is the current state of the Angular 2 router? 46:08 - Any really good recent D3/Angular resources? What are some things to look at in regards to performance when implementing D3 with Angular? Aysegul Yonet and Patrick Stapleton: Creating d3 components with Angular2 and TypeScript @ ng-vegas 2015 49:40 - How do you structure a really large Angular application? John Papa’s Angular Style Guide Picks Angular 2 (Patrick) Angular Tips (Patrick) Observables and the Angular 2 HTTP library (Rob) Go find yourself a local Hackathon (Ward) Visual Studio Code (John) Rob’s Angular Remote Conf talk (Lukas) The Introduction to Reactive Programming series on Egghead.io (Lukas) Periscope (Chuck) Magnus SnapPod Compact Tabletop Tripod (Chuck) ChargerCity MegaGrab2 Easy-Adjust Smartphone Holder Mount & 360º Swivel Adjustment Selfie Video Recording Camera Tripod Adapter (Chuck)

02:39 - When is Angular 2 out? Do you feel like other frameworks (React) are becoming more popular and that people are moving over to them? Will people come back to Angular? 21:01 - How can you convince a client to pick Angular over something else like React? 26:19 - Should I continue learning Angular 1 or move on to Angular 2? Will there still be jobs where I can use Angular 1 after Angular 2 is released? 27:38 - To Typescript or not to Typescript? 33:02 - How do you integrate third-party libraries like Firebase or D3 into an NG2 app, taking into account that NG2 runs on one-way dataflow paradigm fueled by an Rx implementation? 36:14 - The Typescript autocomplete/help, etc. that we were discussing before: what tools or IDEs are we talking about? Visual Studio Code 38:26 - What is the current state of the Angular 2 router? 46:08 - Any really good recent D3/Angular resources? What are some things to look at in regards to performance when implementing D3 with Angular? Aysegul Yonet and Patrick Stapleton: Creating d3 components with Angular2 and TypeScript @ ng-vegas 2015 49:40 - How do you structure a really large Angular application? John Papa’s Angular Style Guide Picks Angular 2 (Patrick) Angular Tips (Patrick) Observables and the Angular 2 HTTP library (Rob) Go find yourself a local Hackathon (Ward) Visual Studio Code (John) Rob’s Angular Remote Conf talk (Lukas) The Introduction to Reactive Programming series on Egghead.io (Lukas) Periscope (Chuck) Magnus SnapPod Compact Tabletop Tripod (Chuck) ChargerCity MegaGrab2 Easy-Adjust Smartphone Holder Mount & 360º Swivel Adjustment Selfie Video Recording Camera Tripod Adapter (Chuck)

This episode is a follow-up episode of Adventures in Angular Episode #48: The Road to NG2   Also, don’t forget to get your Angular Remote Conf Tickets! The online/completely remote conference will run from Thursday, September 24th thru Saturday, September 26th.   03:18 - Panelist Recent Experimentation 06:25 - ES6 vs Typescript, Tooling Dan Wahlin and John Papa Bringing Their View On The Latest In Angular @ Angular U 2015 Atom Visual Studio Code Webstorm Grunt / Gulp 11:21 - Destructuring     Destructuring and parameter handling in ECMAScript 6 16:01 - Debugging 17:07 - Angular 1 => 2 MVC Key Features Needed Getting in the Front Door (Getting Past the Ecosystem) Angular 1 and Angular 2 integration: the path to seamless upgrade 27:32 - Angular 2 & ES5 Pascal Precht: Even better ES5 code for Angular 2 29:44 - Components, Annotations 32:45 - Editors: What Microsoft Users Are Doing TypeScript-Sublime-Plugin atom-typescript 38:35 - Learning Lessons (From Panelists) Picks Angular Articles by Pascal Precht (Lukas) Enter the ng-conf ticket lottery (Joe)  

This episode is a follow-up episode of Adventures in Angular Episode #48: The Road to NG2   Also, don’t forget to get your Angular Remote Conf Tickets! The online/completely remote conference will run from Thursday, September 24th thru Saturday, September 26th.   03:18 - Panelist Recent Experimentation 06:25 - ES6 vs Typescript, Tooling Dan Wahlin and John Papa Bringing Their View On The Latest In Angular @ Angular U 2015 Atom Visual Studio Code Webstorm Grunt / Gulp 11:21 - Destructuring     Destructuring and parameter handling in ECMAScript 6 16:01 - Debugging 17:07 - Angular 1 => 2 MVC Key Features Needed Getting in the Front Door (Getting Past the Ecosystem) Angular 1 and Angular 2 integration: the path to seamless upgrade 27:32 - Angular 2 & ES5 Pascal Precht: Even better ES5 code for Angular 2 29:44 - Components, Annotations 32:45 - Editors: What Microsoft Users Are Doing TypeScript-Sublime-Plugin atom-typescript 38:35 - Learning Lessons (From Panelists) Picks Angular Articles by Pascal Precht (Lukas) Enter the ng-conf ticket lottery (Joe)  

This episode is a follow-up episode of Adventures in Angular Episode #48: The Road to NG2   Also, don’t forget to get your Angular Remote Conf Tickets! The online/completely remote conference will run from Thursday, September 24th thru Saturday, September 26th.   03:18 - Panelist Recent Experimentation 06:25 - ES6 vs Typescript, Tooling Dan Wahlin and John Papa Bringing Their View On The Latest In Angular @ Angular U 2015 Atom Visual Studio Code Webstorm Grunt / Gulp 11:21 - Destructuring     Destructuring and parameter handling in ECMAScript 6 16:01 - Debugging 17:07 - Angular 1 => 2 MVC Key Features Needed Getting in the Front Door (Getting Past the Ecosystem) Angular 1 and Angular 2 integration: the path to seamless upgrade 27:32 - Angular 2 & ES5 Pascal Precht: Even better ES5 code for Angular 2 29:44 - Components, Annotations 32:45 - Editors: What Microsoft Users Are Doing TypeScript-Sublime-Plugin atom-typescript 38:35 - Learning Lessons (From Panelists) Picks Angular Articles by Pascal Precht (Lukas) Enter the ng-conf ticket lottery (Joe)  

The HoloLens epiphany, Adventures in Angular Podcast and The Road to NG2, Agile IT Organization Design and more...

01:46 - Dan Wahlin Introduction Twitter GitHub Blog The Wahlin Group Pluralsight Author Page 02:29 - Background and Involvement in the Angular Community [YouTube] Dan Wahlin: AngularJS in 20ish Minutes (ng-conf 2014) [YouTube] TypeScript and ES6 Dan Wahlin & Andrew Connell (ng-conf2015) 04:16 - TypeScript TypeScript Source Code 06:02 - Why Care About TypeScript? 07:20 - ES3, ES5, ES6 10:00 - Type Support 11:41 - Refactoring 12:39 - Microsoft Involvement Open Source Source Open (Pull Request Acceptance) 17:45 - Benefits and Concerns .d.ts tslint 20:07 - TypeScript and Angular Directives and Providers Services vs Factories Functional Programming 24:11 - TypeScript and Angular 2 Angular.io 25:28 - Collaboration (AtScript => TypeScript) Annotations and Naming Conventions 30:47 - The Angular Community and TypeScript Tooling and Transpiling Babel traceur WebStorm 36:38 - Type Inference ng-flow Picks Avengers: Age of Ultron (John) Star Wars: Episode VII - The Force Awakens (John) .d.ts (John) Lord of the Rings (Katya) Avengers: Age of Ultron (Katya) Matterhorn: A Novel of the Vietnam War by Karl Marlantes (Aaron) Tyler Russell: An Angular2 Timezone Picker - Part 1: Becoming a Kartograph-er (Aaron) Tyler Russell: An Angular2 Timezone Picker - Part 2: Exploring the World (of Ng2) (Aaron) [Pluralsight] TypeScript Fundamentals by John Papa and Dan Wahlin (Lukas) DefinitelyTyped (Ward) Kent Meyers: The Quietest Place in the Universe: Digging For Dark Matter in An Abandoned Mine (Ward) Daredevil (Joe) GoFundMe (Joe) [GoFundMe] Send Samantha to Miss Amazing! (Joe) Headspace (Dan) Faker.js (Dan)

01:46 - Dan Wahlin Introduction Twitter GitHub Blog The Wahlin Group Pluralsight Author Page 02:29 - Background and Involvement in the Angular Community [YouTube] Dan Wahlin: AngularJS in 20ish Minutes (ng-conf 2014) [YouTube] TypeScript and ES6 Dan Wahlin & Andrew Connell (ng-conf2015) 04:16 - TypeScript TypeScript Source Code 06:02 - Why Care About TypeScript? 07:20 - ES3, ES5, ES6 10:00 - Type Support 11:41 - Refactoring 12:39 - Microsoft Involvement Open Source Source Open (Pull Request Acceptance) 17:45 - Benefits and Concerns .d.ts tslint 20:07 - TypeScript and Angular Directives and Providers Services vs Factories Functional Programming 24:11 - TypeScript and Angular 2 Angular.io 25:28 - Collaboration (AtScript => TypeScript) Annotations and Naming Conventions 30:47 - The Angular Community and TypeScript Tooling and Transpiling Babel traceur WebStorm 36:38 - Type Inference ng-flow Picks Avengers: Age of Ultron (John) Star Wars: Episode VII - The Force Awakens (John) .d.ts (John) Lord of the Rings (Katya) Avengers: Age of Ultron (Katya) Matterhorn: A Novel of the Vietnam War by Karl Marlantes (Aaron) Tyler Russell: An Angular2 Timezone Picker - Part 1: Becoming a Kartograph-er (Aaron) Tyler Russell: An Angular2 Timezone Picker - Part 2: Exploring the World (of Ng2) (Aaron) [Pluralsight] TypeScript Fundamentals by John Papa and Dan Wahlin (Lukas) DefinitelyTyped (Ward) Kent Meyers: The Quietest Place in the Universe: Digging For Dark Matter in An Abandoned Mine (Ward) Daredevil (Joe) GoFundMe (Joe) [GoFundMe] Send Samantha to Miss Amazing! (Joe) Headspace (Dan) Faker.js (Dan)

01:46 - Dan Wahlin Introduction Twitter GitHub Blog The Wahlin Group Pluralsight Author Page 02:29 - Background and Involvement in the Angular Community [YouTube] Dan Wahlin: AngularJS in 20ish Minutes (ng-conf 2014) [YouTube] TypeScript and ES6 Dan Wahlin & Andrew Connell (ng-conf2015) 04:16 - TypeScript TypeScript Source Code 06:02 - Why Care About TypeScript? 07:20 - ES3, ES5, ES6 10:00 - Type Support 11:41 - Refactoring 12:39 - Microsoft Involvement Open Source Source Open (Pull Request Acceptance) 17:45 - Benefits and Concerns .d.ts tslint 20:07 - TypeScript and Angular Directives and Providers Services vs Factories Functional Programming 24:11 - TypeScript and Angular 2 Angular.io 25:28 - Collaboration (AtScript => TypeScript) Annotations and Naming Conventions 30:47 - The Angular Community and TypeScript Tooling and Transpiling Babel traceur WebStorm 36:38 - Type Inference ng-flow Picks Avengers: Age of Ultron (John) Star Wars: Episode VII - The Force Awakens (John) .d.ts (John) Lord of the Rings (Katya) Avengers: Age of Ultron (Katya) Matterhorn: A Novel of the Vietnam War by Karl Marlantes (Aaron) Tyler Russell: An Angular2 Timezone Picker - Part 1: Becoming a Kartograph-er (Aaron) Tyler Russell: An Angular2 Timezone Picker - Part 2: Exploring the World (of Ng2) (Aaron) [Pluralsight] TypeScript Fundamentals by John Papa and Dan Wahlin (Lukas) DefinitelyTyped (Ward) Kent Meyers: The Quietest Place in the Universe: Digging For Dark Matter in An Abandoned Mine (Ward) Daredevil (Joe) GoFundMe (Joe) [GoFundMe] Send Samantha to Miss Amazing! (Joe) Headspace (Dan) Faker.js (Dan)

We assessed whether Angiopoietin-2 (Ang2), a Tie2 ligand and partial antagonist of Angiopoietin-1 (Ang1), is required for early vessel destabilization during postischemic angiogenesis, when combined with vascular growth factors. In vitro, matrigel co-cultures assessed endothelial-cell tube formation and pericyte recruitment after stimulation of VEGF-A, Apelin (APLN), Ang1 with or without Ang2. In a murine hindlimb ischemia model, adeno-associated virus (rAAV, 3×10(12) virusparticles) transduction of VEGF-A, APLN and Ang1 with or without Ang2 (continuous or early expression d0-3) was performed intramuscularly (d-14). Femoral artery ligation was performed at d0, followed by laser doppler perfusion meassurements (LDI) 7 and 14. At d7 (early timepoint) and d14 (late timepoint), histological analysis of capillary/muscle fiber ratio (CMF-R, PECAM-1) and pericyte/capillary ratio (PC-R, NG2) was performed. In vitro, VEGF-A, APLN and Ang1 induced ring formation, but only APLN and Ang1 recruited pericytes. Ang2 did not affect tube formation by APLN, but reduced pericyte recruitment after APLN or Ang1 overexpression. In vivo, rAAV.VEGF-A did not alter LDI-perfusion at d14, consistent with an impaired PC-R despite a rise in CMF-R. rAAV.APLN improved perfusion at d14, with or without continuous Ang2, increasing CMF-R and PC-R. rAAV.Ang1 improved perfusion at d14, when combined with rAAV.Ang2 (d0-3), accompanied by an increased CMF-R and PC-R. The combination of early vessel destabilization (Ang2 d0-3) and continuous Ang1 overexpression improves hindlimb perfusion, pointing to the importance of early vessel destabilization and subsequent vessel maturation for enhanced therapeutic neovascularization.

In this thesis a novel method to isolate and cultivate pericytes from hamster skeletal muscle is presented. By choosing a two-step approach with magnetic-bead cell sorting for the 3G5 antigen and culture in a selective growth medium a high percentage of pericytes could be reached and their nature could be verified in subsequent characterization steps. Morphologically the cells had a typical phenotype with flat and large cell bodies and long protruding processes. Immunocytochemistry and western blotting could confirm the expression of the previously reported pericyte markers NG2, PDGFR-β, and αSMA. However, in accordance with previous reports, even the cells isolated from the same muscle and tissue, were not a uniform population. A certain heterogeneity was also present in the functional studies investigating pericyte membrane channels by electrophysiology. In these experiments in a subset of the pericyte population the presence of the voltage sensitive potassium channel KV1.5 could be demonstrated. Upon pharmacological stimulation of these channels by FFA they were able to elicit a membrane hyperpolarization and conduct it to neighboring endothelial cells via gap junctions formed by connexin 43. The expression of the KV1.5 channel could also be demonstrated by immunohistochemistry in paraffin sections from hamster skeletal muscle. These findings are consistent with the hypothesis that pericytes could act as signal generators for hyperpolarization and vasodilatation in the vessel wall. The coupling with endothelial cells might relay an electric signal further along the vessel wall and lead to “EDHF mediated” dilations [48]. Interestingly in tissue sections KV1.5 positive pericytes were located predominantly at vascular branchings. We additionally studied whether gap junctions may also form a pathway for the exchange of miRNA between cells in a model co-culture system using HeLa. For the detection of the miRNA a detector system consisting of a luciferase reporter protein under control of a miR124 sensitive 3’ UTR was established. Our experiments could not conclusively confirm transfer of miRNA in this system. Similar results were obtained with human miR15b. Taken together these findings open up new possibilities for studying skeletal muscle pericyte physiology and to test novel concepts integrating the pericyte into the vascular signaling network under conditions of health and disease.

Some glial cells can generate action potentials and are hypersensitive to ischemic injury.