Podcasts about culturing

AABP Executive Director Dr. Fred Gingrich is joined by Dr. Justine Britten, a PhD animal scientist working at Udder Health Systems Inc. This episode is brought to you by the AABP Milk Quality and Udder Health Committee. AABP members with an interest in milk quality are invited to volunteer to serve on the committee. Find out more information about the committee by visiting this page.   Staph aureus is a contagious mastitis pathogen and Britten states that every dairy is affected to some degree by Staph aureus with a huge range in severity and prevalence between herds. Veterinarians play a key role in developing monitoring and prevention programs. Culturing remains a key point in identifying animals that are affected with Staph aureus so they can be evaluated for culling, segregation or treatment. Cultures should focus not only on clinical animals because Staph aureus is commonly a subclinical infection. It is estimated that 2-15% of heifers will freshen with the infection and their somatic cell count may be low for some time before it begins to climb. Treatment should be reserved for heifers and it is critical to identify it early in the course of infection to increase chance of treatment success. Britten states that in most circumstances, there is no need to do sensitivity testing on milk cultures unless there is an outbreak situation with apparent treatment failures. Bulk tank cultures are important and a minimum for routine herd mastitis monitoring. The sensitivity of bulk tank cultures is low but serial positive silos from a dairy indicate a higher prevalence of the infection up to 5-10% of the cows.  We also discuss that in outbreak situations it is important to evaluate why cows are getting infected and remember that cows typically get infected in the parlor and focus efforts there. This includes evaluating teat condition, parlor procedures and equipment function. Aggressive culturing programs, segregation and culling are important to minimize risk.  Britten provides these three take-home points for veterinarians:Take-home point #1 – Not all Staph aureus colonies exhibit beta-hemolysis, therefore, it is important that all Staph colonies undergo coagulase testing to identify Staph aureus.Take-home point #2 – Monthly bulk tank cultures are a bare minimum monitoring program for dairy farms.Take-home point #3 – Somatic cell count is a lagging indicator and cultures will detect infections earlier.Mastitis is the number one disease of dairy cattle and should be involved in milk quality and mastitis prevention programs. The Milk Quality and Udder Health Committee has developed guidelines for milk quality service providers. Find this document under the Committee Resources page, click on the Milk Quality and Udder Health page, then the Committee Resources tab to find these documents. 

In this episode of the ICHE podcast, we explore the theme of respiratory culturing appropriateness in pediatric patients, emphasizing diagnostic stewardship's role in optimizing patient outcomes. Host David Calfee, MD, MS and guest experts, Kathleen Chiotos, MD, MSCE and Edward Lyon, DO, MA, share insights on how stewardship practices can refine respiratory testing in pediatric intensive care units (PICUs), where evidence suggests these tests are often overused without clinical benefit. They examine challenges in implementing stewardship, including barriers like resistance to change and logistical issues, and discuss practical strategies to overcome these obstacles. The conversation highlights two recent studies published in ICHE: Dr. Lyon's work on repeat tracheal aspirate cultures in PICUs, which analyzes their frequency, resistance patterns, and impact on antimicrobial use, and Dr. Chiotos's study on a tracheal aspirate culture stewardship intervention, examining its effect in a tertiary PICU. Both authors discuss their studies' methodologies, findings, limitations, and implications for future practice. To wrap up, each expert offers actionable recommendations for healthcare professionals interested in improving respiratory culturing practices at their institutions, providing practical steps to enhance safety and care efficiency. Full versions of the articles discussed in this episode are available in the September issue of ICHE.

Slappin' Glass sits down this week with the Head Coach of Lipscomb MBB, Lennie Acuff! Coach Acuff is one of the best teachers in the game and the trio dive into his thoughts on fixing "5 Out" spacing, the benefits of a great "toss" game, and discuss defending dominant post players and the "out culturing" opponents during the always interesting "Start, Sub, or Sit?!"To join coaches and championship winning staffs from the NBA to High School from over 60 different countries taking advantage of an SG Plus membership, visit HERE!

Episode Description: Karl and Erum welcome Tony Kulesa, an engineer, investor, and entrepreneur in biotech. He's sharing his insights on the future of biotechnology, discussing how advancements in reading and writing biology are driving significant changes in the field. Tony recounts his journey from academia to founding Petri, an incubator for biotech startups, and later integrating it into Pillar VC to support a global community of scientist-entrepreneurs. Tony emphasizes the importance of a supportive culture for transitioning academic research into practical, impactful solutions. The discussion covers the challenges and opportunities in biopharma, the potential of biotech in addressing global issues, and the growing interest in animal healthcare. Tony's perspectives are both inspiring and practical, providing valuable guidance for aspiring biotech entrepreneurs. Grow Everything brings the bioeconomy to life. Hosts Karl Schmieder and Erum Azeez Khan share stories and interview the leaders and influencers changing the world by growing everything from sustainable ingredients to high performing materials. Biology is the oldest technology. What are we growing? JOIN OUR BIOCOMMUNITY. BECOME A PATREON.  START HERE. Chapters: 00:00:00 - Unlocking the Power of Biology: A New Era 00:00:15 - Summer Vibes and Outdoor Adventures 00:02:19 - Biotech Events and Networking in the Summer 00:03:00 - Dissecting Biopharma Events and AI in Drug Discovery 00:05:11 - The Future of AI and Drug Discovery: Insights and Disappointments 00:09:17 - Investment Trends and Opportunities in Biopharma 00:15:51 - Building a Community: The Grow Everything Podcast Patreon 00:17:36 - Introducing Tony Kulesa: From Research to Venture Capital 00:23:00 - Tony Kulesa on Microbial Therapeutics and Drug Discovery Platforms 00:26:22 - Tony Kulesa's Journey from Research to Venture 00:32:39 - Entrepreneurship at MIT: A Cultural Shift 00:34:42 - COVID-19 and Its Effect on Global Startups 00:37:50 - Tony Kulesa on Scientist-Founders: The New Wave in Biotech 00:40:15 - The Role of Culture in Startup Success 00:42:45 - Insights on Team Building and Leadership in Biotech Startups 00:44:00 - Navigating Regulatory Pathways for Biotech Innovations 00:45:15 - Importance of Networking and Advisory Support in Biotech 00:46:40 - Exploring New Frontiers: Biotech Beyond Medicine 00:47:52 - The Potential of Animal Healthcare in Biotech 00:49:37 - Global Expansion and the Future of Biotech Entrepreneurship 00:50:55 - Addressing Global Challenges Through Biotech Innovations 00:57:03 - Reflections and Future Directions in Biotech Entrepreneurship Episode Links: Tony Kulesa on LinkedIn (link) Pillar VC (link) Synbiobeta (link) Ayana Bio (link) Concerto Biosciences (link) Traitomic (link) OpenBiome (link) Amber Bio (link) Thymmune (link) Topics Covered: biotech funding, biopharma, venture capital, biology, startup, entrepreneurship, synthetic biology, MIT, Harvard Have a question or comment? Message us here: Text or Call (804) 505-5553 ⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠Instagram⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠ / ⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠TikTok⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠ / ⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠Twitter⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠ / ⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠LinkedIn⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠ / ⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠Youtube⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠ / ⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠GrowEverything website⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠ Email: groweverything@messaginglab.com Music by: Nihilore Production by: Amplafy Media --- Send in a voice message: https://podcasters.spotify.com/pod/show/messaginglab/message

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Episode Description: Erum and Karl welcome renowned environmentalist, Murali Muthuswamy. He is the founder and chairman of Jananom Private Limited, founder of Elive Biotherapeutics USA, and advisor to Natural Nutrition. Murali explains the urgent and complex challenges of sustainable development. He also talks about the intricate balance required to meet the needs of the present without compromising the future. Exploring innovative solutions, Murali shines a light on groundbreaking technologies and community-driven initiatives that are paving the way for a more sustainable world. This episode not highlights the critical issues but also inspires action, making it a must-listen for anyone interested in shaping a sustainable future. Grow Everything brings the bioeconomy to life. Hosts Karl Schmieder and Erum Azeez Khan share stories and interview the leaders and influencers changing the world by growing everything. Biology is the oldest technology. And it can be engineered. What are we growing? Learn more at ⁠⁠⁠⁠⁠⁠⁠www.messaginglab.com/groweverything⁠⁠⁠⁠⁠⁠⁠ Chapters: 00:00:00 - Opening Welcome to Grow Everything: Diving into Today's Special Guest Intro with Shelby Newsad 00:01:29 - Global Explorations: Uncovering Insights from Australia and Brazil 00:04:15 - Climate Biotech Symposium: A Nexus of Networking and Innovation 00:11:32 - Sustainable Innovations: Case Studies of Success 00:13:12 - Trailblazing Paths: Murali Muthuswamy's Entrepreneurial Saga 00:17:04 - From Vision to Reality: Scaling Sustainable Solutions 00:20:21 - The Role of Policy in Supporting Eco-Innovations 00:24:42 - At the Cutting Edge: Synthetic Biology's Battle Against Cancer 00:26:50 - Green Tech: Harnessing Renewable Energy Sources 00:30:33 - Eco-Friendly Manufacturing: Reducing Carbon Footprints 00:34:10 - The Economic Impact of Sustainable Practices 00:38:39 - Innovative Approaches to Oncology: Smart Microorganisms at Work 00:40:17 - Redefining Pharmaceuticals: The Synthesis of Biology and Drug Development 00:41:52 - Exploring the Promise of Living Biotherapeutics 00:45:03 - Digital Meets Biological: Transforming Research and Applications 00:50:12 - India's Ambitious Stride into Biomanufacturing 00:52:20 - Fostering Future Leaders in Biotech Entrepreneurship 00:55:50 - In the Limelight: Breakthroughs from Leading Biotech Firms 00:58:44 - Converging Worlds: Digital Integration in Modern Biotechnology 01:01:15 - Conclusion: Celebrating the Advances in Biotech Innovation Episode Links: Compound VC (link) Genspace  (link) Evolva (link) India Institute of Chemical Technology (link) Department of Biotechnology in India (link) Biocon (link) Tata Elxsi (link) Avisa Myko (link) Synonym Biomanufacturing (link) Scaling Cells, Dreaming Big: The Biomanufacturing Cloud with Synonym's Edward Shenderovich (link) Homeworld Collective (link) Cascade Biocatalysts (link) SentiBio (link) Encellin (link) Get $300 off ⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠Synbiobeta tickets⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠ (May 6-9 in San Jose, CA) using promo code: Grow Everything Topics Covered:  synthetic biology, synbio, bioengineering, organism engineering, microbiology Have a question or comment? Message us here: Text or Call (804) 505-5553 ⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠Instagram⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠ / ⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠TikTok⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠ / ⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠Twitter⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠ / ⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠LinkedIn⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠ / ⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠Youtube⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠ / ⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠GrowEverything website⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠ Email: groweverything@messaginglab.com Support here: ⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠Patreon⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠ Music by: Nihilore Production by: Amplafy Media --- Send in a voice message: https://podcasters.spotify.com/pod/show/messaginglab/message

In this webinar, Katie Flaherty presented strategies and classroom resources to bring together History, Art, Archaeology, and Culture into the Foreign Language classroom through a sample Latin curriculum ranging from grades 6-12. Flaherty also discussed additional pedagogical resources for increasing teacher confidence and knowledge on these topics outside the classroom.Mrs. Flaherty received a B.A. in Classics and a B.A. in Anthropology from the University of Tennessee - Knoxville, a Post-Baccalaureate Certificate in Classical Languages from the University of California - Los Angeles, and a M.A. in Classics from Florida State University. Mrs. Flaherty teaches Latin.

In this episode, Jon talks to Dr. Julia Swavola, the head of Product at the company Cerillo. Julia talks with us about her experiences her career. She also talks about Cerillo,  the mission, what products the company makes and their applications, collaborations, and what's on the horizon with the company.  We wanted to thank both Julia and Cerillo for the opportunity to talk with them.   If you are interested in their products or are interested in learning more about the company checkout their website here or on Twitter (X) by searching for @cerillobio.  Visit our website at microbigals.com where we regularly update new content, including blog posts. You can also find us on Instagram by searching for microbigals or on Twitter @MicrobiGals. Also, if you want to donate to our show, we have started a kofi page. you can donate on our website or visit https://ko-fi.com/microbigals.

For a patient with sepsis (an infection in the blood), the chance of survival decreases by 7-10% every hour. It's important to quickly identify what bacteria are present and which antibiotics might be effective.Typically, there are very few bacteria in the blood. Culturing bacteria from a sample can take 12-24 hours and then one needs to test for drug susceptibility. Speeding up this process would save a lot of lives.Jen Dionne and her collaborators at Stanford are working on this problem using Raman spectroscopy. Through a combination of nanophotonics, acoustic bioprinting, and machine learning, they are developing methods to quickly and sensitively identify bacteria and screen for antibiotic susceptibility.What is Raman Spectroscopy?Raman spectroscopy is basically inelastic photon scattering. So if you think about having a laser or some other monochromatic light source, you shine that onto your sample, say onto a cell, like a bacterial cell. And the molecular vibrations in that sample basically add to or subtract from the energy of the laser source.You wind up with a fingerprint or a series of scattered wavelengths that are different from your incident wavelength. And you can use that spectrum to identify the constituents of what's in your sample.Raman spectra of complex mixtures appear as a wavy line, more like a snapshot of an oscillating jump rope than a series of distinct peaks that you would get from a pure sample of a small molecule.Here is what you need to know:* It's possible to do Raman spectroscopy on whole cells. By training machine learning models on thousands of bacterial samples, Raman can identify different bacterial species and determine which ones are resistant to specific antibiotics.* Raman is typically not very sensitive but the efficiency goes up by the 4th power of the electric field strength. Surface Enhanced Raman Spectroscopy (SERS) using nanoparticles made of superconducting materials can focus light energy to a very small region without heating and destroying the sample, boosting the sensitivity. The added sensitivity allows the collection of more data (better resolution, more specificity). * Spectra can be collected in flight as droplets (of blood for example) are acoustically ejected at kilohertz frequencies and printed on a substrate. The identification of bacteria by the spectra can be confirmed by electron microscopy of printed drops on the substrate. Rapid diagnosis of sepsis is just one possible application. Analysis of environmental samples and wastewater epidemiology are also possible with this method.The takeaway for me is that with a large enough data set of samples, machine learning algorithms can identify subtle features that would never be possible for a human being. Adding more data through nanophotonics opens up even more possibilities to tackle bigger problems.More about SERSYour deepest insights are your best branding. I'd love to help you share them. Chat with me about custom content for your life science brand.Cover Image Credit (energy states): Moxfyre, based on work of User:Pavlina2.0, CC BY-SA 3.0, via Wikimedia Commons This is a public episode. If you would like to discuss this with other subscribers or get access to bonus episodes, visit cclifescience.substack.com

Culturing, sculpturing the AA readings. Makes for a peaceful, productive life. No alcohol. --- Support this podcast: https://podcasters.spotify.com/pod/show/fernando-m-de-oca/support

Join us for an engaging conversation with Steve Eddy and Luz Kogson from the Center for Cooperative Aquaculture Research (CCAR) in Franklin, ME as they dive into the captivating world of sea urchin aquaculture. In this episode, we explore the growth stages of sea urchins, their rich history in Maine, the culinary delights they offer, and the exciting ongoing projects at CCAR. Discover the remarkable journey of sea urchins from larval form to adulthood, learn about Maine's role as a hub for sea urchin farming, and tantalize your taste buds with insights into their unique flavors and culinary applications.

Adam from Splendid Sports joins us this week to chop up some hobby topics, answers why he's from Massachusetts and is a Mantle fan and the content he produces on his YouTube channel. Fun Convo for sure!Follow us on Social Media: Website:https://www.sportscardnationpo... https://linktr.ee/Sportscardna...

Today our guest is Steve Orrin. Steve is a respected IT executive with over 30 years of experience in cybersecurity, solution architecture, virtualization security, and federal project management. Orrin earned a Bachelor's in Honors Research Biology from Kean University. He currently serves as a Federal Chief Technology Officer and Senior PE for Intel Corporation. He is also a key advisor and subject matter expert in the emerging technologies space, providing guidance to the Public Sector, Defense, and Intelligence communities. [Dec 19, 2022]    00:00 – Intro  00:55 – Intro Links:  Social-Engineer.com - http://www.social-engineer.com/  Managed Voice Phishing  Managed Email Phishing Adversarial Simulations -  Social-Engineer channel on SLACK  CLUTCH innocentlivesfoundation.org  03:43 – Steve Orrin Intro  04:26 – How did a biologist become a CTO at Intel?  09:10 – The evolution of security attacks  12:22 – Humans are humans  13:18 – Higher-risk targets  13:54 – If I knew then...LISTEN!  15:09 – Speak the language  16:27 – Making limoncello out of lemons  17:36 – Check your ego  19:04 – The prerequisites  21:06 – A Self-fulfilling Prophecy of Awesome Things  22:15 – Perfection is the Enemy of Good  26:06 – Handling failure  28:12 – Culturing opportunities  30:37 – Ninja skills  32:07 – Have you had mentors in your career?  Bruce Schneier  David Riss  33:42 – Book Recommendations   Good to Great – Jim Collins  Built to Last – Jim Collins  Applied Cryptography – Bruce Schneier  35:52 – Find Steve Orrin online  LinkedIn: www.linkedin.com/in/sorrin/  intel.com/publicsector  37:02 – Wrap Up  37:21 – Outro  www.social-engineer.com  www.innocentlivesfoundation.org 

David Levine joins Megan to speak about bacterial culturing in canine rehab. David has such a big passion for microbiology and gives us solid advice on how to minimalize outbreaks.  Learn more about David Levine: https://en.m.wikipedia.org/wiki/David_F._Levine Learn more about Respond Systems: https://respondsystems.com/ To learn about Onlinepethealth, watch a free webinar, or join any of our Facebook groups, click here: https://onlinepethealth.com/podcast

During the Global Ecovillage Network Europe Conference in 2022 in Ananda Gaorii, Denmark, the Possibility Management Team had the privilege to meet Dougald Hine. After his keynote speech in the Main Hall, we grabbed him for a more personal and in-depth interview with Clinton Callahan, where he could tell us the real stuff... We add this more recent interview to the Gaian Road Team project: each impulse leading us to make inexplicably coincidental meetings with the Edgeworkers, Social Alchemists, Riftwalkers, Bridge Builders, and Possibilitators who are inventing and inhabiting Archiarchy. These remarkable beings commit to themselves as much as to the novel outcomes they are creating. They build-out new gameworlds and inhabit them. They trust discoveries from their own research and freely share everything they have learned for the benefit of all. We are so happy to share these intimate conversations from around the world with you. Here you get to meet so many radiant next-culture Beings in our Gaian Road Team Interviews. We hope you become as inspired and hopeful as we are about what is possible. Related websites: https://dougald.nu http://clintoncallahan.org http://gaianroadteam.org http://spaceport.mystrikingly.com http://adulthood.mystrikingly.com http://possibilitymanagement.org http://possibilitatortraining.org http://gaiangameworlds.org http://sparkexperiments.org http://distinctionary.xyz http://nextcultureradio.org http://archiarchy.org http://initiations.org http://startover.xyz

The film version of Cole Porter’s show, which is based on Shakespeare’s “Taming of the Shrew” (got that?) landed in the middle of MGM’s big technicolor musical era, the 1950s. Howard Keel, Kathryn Grayson and Ann Miller are your stars, and a very young Bob Fosse is among the standouts in the cast. Movie censors got to Porter’s lyrics, which gives us some fun topics to cover. It’s also your host’s birthday show. Because it’s too darn hot to cover anything else. Shelly Brisbin with David Almeida, Annette Wierstra and David J. Loehr.

The film version of Cole Porter’s show, which is based on Shakespeare’s “Taming of the Shrew” (got that?) landed in the middle of MGM’s big technicolor musical era, the 1950s. Howard Keel, Kathryn Grayson and Ann Miller are your stars, and a very young Bob Fosse is among the standouts in the cast. Movie censors got to Porter’s lyrics, which gives us some fun topics to cover. It’s also your host’s birthday show. Because it’s too darn hot to cover anything else. Shelly Brisbin with David Almeida, Annette Wierstra and David J. Loehr.

This week on our culturing creativity episode, we explored Sestina Poems and discussed how they can contribute to inspiring creativity. For this exercise, we used the instructions on the masterclass website linked below to come up with the Sestina Poem. Adhering to the strict structure was a challenge, but an enjoyable one! Click on the link if you are interested in creating a sestina poem yourself. https://www.masterclass.com/articles/sestina-poem-form Below, you can find our poems. Sarah's Poem: A Sestina for Julia Nothing more fun than play with your favourite person. A toothless grin, your face an open book. Between your gums, pools milk, overflowing down your chin. As we sing your eyes flutter closed. The parks are closed. Mummy, where can we play? At home, my sweet. Coo and sing to me, with those unpracticed lips. Grin when you make a sound. Bottled milk makes wet rings, resting on your book. Too tired for a book. Slumbering with the door closed, you won't stir for milk. Tickling your toes, your nose. I play with your hands. A sleepy grin. But then you wake, and furiously sing. Beep, beep, beep. The bottle warmer will sing for us. Have patience. Maybe a story? A book? Your frustrated pout is an upside-down grin. Little fists clenched tightly closed, I cannot persuade you to play. You wail, demanding milk. Now satiated, I watch you snooze. Creamy milk- colored skin. How your beauty does sing! Eyelashes longer than mine, they play across rosy cheeks. So many things my book never told me. I press it closed, my chest swelling. If only hearts could grin. Sometimes it grows, your grin. Mouth full with a bottle of milk. Dimples denting, and lips barely closed around the nipple. Just wait until you can sing— why waste a moment with a book, when the world is ready to play? You make my world sing, and I could fill a book about how prettily you play. Ashley's Poem: Reconnection These days, old friends are a memory. Of a time we once connected. Cried together, and laughed. A relic of the past, Where days last only a moment. Gone in no time. Other days, old friends are frozen in time. A picture of a memory. A moment. Two souls connected By a shared history, past. One where we laughed. Like that day we laughed In the bar, that one time. Sharing stories past, And present. A memory, Where we connected, For only a moment. Yesterday, I remembered you for a moment. And I laughed, Like we used to when we connected. I thought back in time To a memory Of our past. Perhaps I should reach into the past And take a moment. To pull out a memory, Of us when we laughed. Together. Bring back a time When we could be connected. Today, again, we connected. And it was like the past Never faded with time. We talked for a moment, Reminisced, and laughed. To make a new memory. Tomorrow I will remember that moment. That time we laughed. The memory.

Opinionated Science is Technology Networks' homemade podcast, where our team of scientists-turned-journalists cut out the chewy jargon and serve up slices of the most fascinating stories from the world of science.  In this sneak peek episode, Lucy Lawrence will discuss the world of cell culturing with Associate Professor of Stem Cell Biology Darius Widera. Dr Widera is currently an Assistant Professor/ Lecturer at the University of Reading, United Kingdom Lucy and Darius will explore how scientists can use cell culturing within their labs, the differences between 2D and 3D cell cultures as well as looking to the future to see where this space is heading next. To listen to the full episode, click here.   

On this culturing creativity episode, we discussed the power of saying 'no'. Saying no can be a difficult feat to master, yet without appropriate boundaries it can be easy to fall victim to burnout. We talked about whether we find it hard to say no, and some tips and thoughts on how to ease the guilt and free up writing time.

It's Q&A time once again.In answer to a young man questioning the timing of parenthood, Thom explains the role the child has in its own incarnation, and reminds us to be led by charm rather than the intellect in our decisions.He also explains how our intellect interferes with our innate connection with the laws of Nature.And he tackles two questions on Cosmic Consciousness. One from a listener seeking clarity on his own experiences of Cosmic Consciousness, and one from another listener about the relationship between stress and Cosmic Consciousness.Something for everyone and, as always, something to inspire us to maintain our practice of Vedic Meditation. Episode Highlights: [00:45] How Do You Know When You're Ready to Be a Parent?[01:30] A Jiva Who Wants to Be Born[03:35] Karma - Unfinished Business[05:02] The Shankaracharya Tradition[07:13] The Only Safe Move[08:28] Great Liberties[09:39] Q- Why Do We Need to Learn or Re-learn the Laws of Nature?[10:00] Hride — The Layer of Us in Connection With Nature[12:12] The Innocent Experience of Charm[14:06] Awakening Our Inborn Talent[15:19] Culturing the Fine Level of Feeling[17:08] Snowed Under By Intellectualism[18:34] Q- Can Practicing Vedic Meditation Twice a Day Bring More Clarity to My Experience?[19:16] The Fortunate Experience of Cosmic Consciousness[20:52] Culturing Your Experience[21:56] Attaining Higher States of Consciousness[23:26] Q- How Does Cosmic Consciousness Become Permanent?[24:08] Cosmic Consciousness is a Natural Condition[25:06] Understanding Stress Properly[27:03] Two Aspects of Stress[30:43] Inability to Accumulate Stress[32:26] A Line in the Sand[33:48] The Experiencer[35:32] I Am Totality Having a Human ExperienceUseful Linksinfo@thomknoles.com https://thomknoles.com/https://www.instagram.com/thethomknoles/https://www.facebook.com/thethomknoleshttps://www.youtube.com/c/thomknoles https://thomknoles.com/ask-thom-anything/

On this Culturing Creativity episode, we try our hand at making mood boards for some of the books in our Black Skies Series and discuss how these mood board can help inspire creative thinking. If you would like to create your own mood board, we suggest using milanote at https://milanote.com for an easy online platform.

Todd & Cathy are joined by two of their daughters (Camryn, 17 & Skylar, 14) to discuss Season 4, Volume 1 of Stranger Things. They discuss how the season was influenced by horror movies from the 80's (Carrie, The Amityville Horror, A Nightmare on Elm Street to name a few….) plus other fan favorites from Generation X (Star Wars, War Games, Fast Times at Ridgemont High, etc). They each choose a song to help them run away from Vecna, process the show's best characters, and share their hottest takes from this season and what comes next.

Todd & Cathy are joined by two of their daughters (Camryn, 17 & Skylar, 14) to discuss Season 4, Volume 1 of Stranger Things. They discuss how the season was influenced by horror movies from the 80's (Carrie, The Amityville Horror, A Nightmare on Elm Street to name a few….) plus other fan favorites from Generation X (Star Wars, War Games, Fast Times at Ridgemont High, etc). They each choose a song to help them run away from Vecna, process the show's best characters, and share their hottest takes from this season and what comes next.

On this culturing creativity episode, we talked all about developing a healthy writing mindset. We explored what a 'writing mindset' is, why it's so important for your writing, and how to work out an approach to writing that is suited to your individual circumstances.

On another culturing creativity episode, we immersed ourselves once again in genre. But this time, we focussed on how to find the genre that suits you best. To some, it seems like an obvious answer. But we discussed how this affects your writing and your confidence, particularly as a new writer if you're not sure where to start.

Brewing, folk arts, circular time, fermentation, and revitalizing regenerative cultures.... we delve into the magic and complexity of brewing with herbalist, singer and folk artist Heather Wolf. About our conversation partner, Heather Wolf:Heather Wolf is an herbal brewess, folk herbalist, teacher, singer & performing artist from an island in the Salish Sea. Her work is devoted to reviving folk wisdom traditions, and a remembrance of our ancestral heritage and sacred relationships. With decades of study and craft of herbal brewing traditions, Heather most recently launched The Brewess Underground, a private brew club centering on dry herbal meads and botanical honey liqueurs, released on the Equinoxes and Solstices. Her herbal brews, including  her honey-kombuchas, have been much beloved throughout the American Northwest. In addition to brewing, she is re-learning her own Sephardic Jewish & British Isles ancestral songlines and partaking in the revitalizing of folk traditions. She writes of folk traditions, "Folk wisdom is carried by the collective, belongs to the whole, is the vital foundation of every healthy culture. These arts relate us directly with our own beings, each other, and the sacred world from which our existence is emergent and interdependent."About this show:00-3:40 - Introductions and initial music 5:28 - How does brewing enable us to unwind in time?6:50 - What is inebriation?8:18 - What is mead?10:48 - What actually happens in fermentation?11:39 Heather's process of fermentation;  thinking about alcohol 15:50: Culturing the culture23:41 How is time interfacing with the creation of a brew?28:40 Some revitalizations in our time32: 10 Brewing and circular time 50:38 Fairytale time45:00 Heather Wolf reading and singing the song, Old Growth Fairytale,  that she co-created with Nicholas Moon and their band, Wild Revival 54:30 Brewing Brewess, and suggestions for your practiceRelevent links: Join our upcoming Circular Time courseFollow Sara Jolena and Sequoia SamanvayaSequoia Samanvaya's WebsiteSequoia Samanvaya's Instagram Sequoia Samanvaya's Facebook Sequoia Samanvaya's LinkedInSara Jolena's LinkedInHeather's website (which includes links to her music)Buy the song Old Growth Fairytale by Wild Revival:Follow Wild Revival on:Instagram:https://www.instagram.com/wildrevivalband/Facebookhttps://www.facebook.com/wildrevivalbandSupport the showLearn more about Sara Jolena Wolcott and Sequoia SamanvayaMusic Title: Both of Us Music by: madiRFAN Don't forget to "like" and share this episode!

On this culturing creativity episode of Dear Writer we tried out and discussed a writing exercise called, "Exploding A Moment". This wonderful exercise helps with developing detailed description in your creative writing. You can find instructions on how to do this exercise here: https://wr.english.fsu.edu/College-Composition/The-Inkwell/Details-and-Descriptions#Exploding%20a%20Moment:%20Developing%20Details

On this Culturing Creativity episode we experimented with Blackout Poetry. We discussed what it is, how to create it and how we found the experience.

This week we threw ourselves headfirst into the topic of self-doubt and Imposter Syndrome. In this episode, we expose our own insecurities and talk about how to manage these feelings as a writer and an author. We also discussed our separate working lives and the lessons we've learned through our day jobs.

Nurture.hf is a not for profit that has been created by Christiana, who is an embryologist, to support patients going through fertility treatments with evidence-based information and facts to support them in their journey. Christiana is passionate about empowering others with information about reproductive and fertility education as we are taught so little about this as part of our education when we are growing up and giving them access to what goes on behind the embryology door and to understand the science behind the process. What's the difference between IVF and ICSI and why would you choose one over the other? We talk about Lab conditions and Male factor infertility and how the advances that have been made are now enabling male factor infertility to be treated more effectively. We talk about fertilisation after egg retrieval and the possible news that you can receive 24 hours after retrieval. We talk about how little we know about our own reproductive health and how this really does need to be included in Sex education and how we are taught everything about how not to conceive and nothing about how to plan for the families we may or may not want to have in the future. The importance of checking our fertility/reproductive health during our twenties would be so beneficial in preventative fertility health care. What tests should we be having for sperm and why? Is one sperm analysis enough? And what abstinence period should we be following and why is the current WHO advice maybe not the best advice for the best sperm sample. The lifestyle and nutrition of the sperm holder is vital in the 74 days leading up to when the sperm needs to be put in for IVF/ISCI. We also talk about how the sperm holder does need to be seen by a Urologist/andrologist and not just by the fertility consultant at the clinic who is going to be specialising in Gynaecology/Endocrinology which are for Female fertility. Sperm DNA fragmentation is now being looked at more closely and it's important to know that it's not always the egg. We should be looking at both Egg and Sperm with as much importance as each other. Ruling issues in and out for both sperm and egg is vital. The tests that females undergo are far more intrusive than the tests and examinations that are required for male party, yet they are looked at far less and often not until we are multiple of failed rounds in. Getting these tests done before treatment can really help shorten our path in fertility treatments. What questions should we be asking the lab at the clinic we are choosing to have treatment at? We talk about extended culture for embryos and how culturing beyond day 5 can still produce blastocyst embryos. How does it feel to deliver news on embryos to patients as the embryologist? Nurture Honest Fertility Links: FACEBOOK, INSTAGRAM, WEBSITE Thank you as always for listening and let us know your thoughts on the episode. You can always leave us a message or ask a question using the message button in the show notes (available on Spotify, Apple and Anchor podcasts) See stories for more on instagram @emmakbelle Join the IVF after loss facebook group HERE Find more about TFMR Mamas Support HERE --- Send in a voice message: https://anchor.fm/all-ivf-ever-wanted/message

Our topic for this week in our 'Culturing Creativity' space was accepting feedback. As a writer, you want to improve and be the best you can be. But sometimes feedback came come across in a negative frame that causes us to put up our defenses. So how do you cope with feedback? Listen to hear our perspective.

This week on our Culturing Creativity series, we explored the uses of modern technology and creativity to determine whether it is a help or hindrance on the way to becoming a published author. We discussed how we employ technology and how (for the most part) we'd be lost without it!

This week we tried a writing exercise, titled the 'Stain Glass Poem'. This involved answering a number of questions and then reorganizing the answers into a poem of sorts. We did the exercise and talked about how it worked for us and how we think it helps to culture creativity. The exercise came from the article: Enhancing the group experience: Creative writing exercise by Kathie Wenz and J. Jeffries McWhirter (1990) in the Journal for Specialists in Group Work, 15:1, p37-42.

This week we learn about growing bacteria and how to safely perform this practical in school.

Inspired by our last culturing creativity episode on games, we decided to focus on one game in particular and try it out to see how it works. The story cubes are a great writing prompt to encourage creative writing and to make connections between different concepts. Listen in to hear more of our experience.

Time to talk about the most widespread infection in the world, Helicobacter pylori. Following the accidental abandonment of incubated plates, H. Pylori colonies were discovered, launching a larger investigation into the microbiology of the human stomach. More research is needed on this disease-causing gram-negative bacterium, but our hosts provide the basics of its characteristics, transmission routes, and prevention in this episode.   More about Helicobacter pylori: Persistence strategies of the bacterial pathogen Helicobacter pylori Gastroenteritis and Transmission of Helicobacter pylori Infection in Households Nobel prize is awarded to doctors who discovered H pylori Pathogen Safety Data Sheets Trends in Hospitalizations for Peptic Ulcer Disease H pylori finds its home  Stay tuned for more episodes, posting on the first Thursday of each month. Subscribe to our show wherever you get your podcasts and find more info at weebeastiespodcast.com   The Wee Beasties podcast is a production of Nephros, Inc., a company committed to improving the human relationship with water through leading, accessible technology. ***   SHOW TRANSCRIPT: Christian: I am back with Dr. Kimothy Smith. Kimothy, welcome back!  Kimothy: Thanks, Christian.  Christian: All right…how are we doing today Kimothy? How's life?  Kimothy: I'm feeling a lot of stress, and I've got a pain in my stomach. I think I'm getting an ulcer.  Christian: Today's the right day to have this conversation, I think. We're talking about pathogens on this podcast, so I hear?  Kimothy: Cool! Let's go.  Christian: I was watching that documentary, Human Nature, last night. Have you seen it yet? It's about genomics, personalized medicine using CRISPR cas9 and the scope of genetic and molecular engineering to cure disease, but also do wild projects like bringing back a wooly mammoth. Cool stuff. Worth a watch if you have some time.  Kimothy: I'll check it out, but I tend to be a bit old school. And, I don't mean to go too deep on the old school stuff here, but, have you seen Jurassic Park? Do you really want to bring back a wooly mammoth?  Christian: What is that line from Jeff Golblum in Jurassic Park? “Your scientists were so preoccupied with whether or not they could, they didn't stop to think if they should?”  Kimothy: Yeah, definitely.  Christian: Well, what's our next waterborne pathogen?  Kimothy: Apropos to my ulcer generation, it is Helicobacter pylori.  Christian: H. pylori, I'm not super familiar with this one, but if memory serves this is spiral-shaped bacteria that makes a home in your stomach and can cause ulcers and even stomach cancer in some cases, right?  Kimothy: Yeah, you're on the right track, Christian. According to a 2013 report1 in Nature, H. pylori is the most widespread infection in the world, infecting at least half of the global population. The World Health Organization (WHO) recognized H. pylori as a group 1 carcinogen in 1994, but the backstory on this little bug isn't that straight forward.  H. pylori was discovered more recently, in comparison to the other microorganisms we've discussed – it was discovered in 1983 – and, interestingly enough, it was first found as a colony in the human stomach in a really unexpected stroke of luck after two doctors were trying to demonstrate a connection between the severity of gastric distress experienced by their patients and the number of bacteria present. In part, this discovery prompted scientists to begin a larger investigation into the microbiology of the human stomach using 16S rRNA analysis – and now we know that the stomach and the rest of the human gut has an extraordinarily diverse microbiome of bacteria which is critical to our immune response and other autonomic faculties. And I must tell you, Christian, as a side note, I've heard an urban legend that your microbiome can determine if you have a sweet tooth or not. Have you heard that?  Christian: I have not, but I've got a horrible sweet tooth. I'm always wanting sweets. So, I'm sort of wondering if we should run a 16S rRNA analysis of my gut biopsy.  Kimothy: Keep your microbiome to yourself, please.  Christian: Yeah, so this was part of what catalyzed the gut microbiome frenzy in the 90's. But wait, what was the stroke of luck? And, if good bacteria is so critical to our gut and immune response how is H. pylori, a pathogenic bacterium, able to stay alive in there?  Kimothy: Yeah, Christian, I'm glad you asked. So, the stroke of luck was in the successful culture. The two Australian doctors credited with the discovery just mistakenly left a plate in the incubator a lot longer than they had intended, and they just happen to get H. pylori colonies on it.  Those that have worked with H. pylori before will know just how persnickety the bacteria is – it is exceedingly difficult to grow outside of its habitat.  Christian: And why is that?  Kimothy: Well, let me get into the weeds a bit here. So, like many of the other pathogens we've discussed, H. pylori is a gram-negative bacterium, which means it has that extra LPS (lipopolysaccharide) barrier on its outer membrane. So, for starters, its more protected simply by its composition as a gram-negative bacterium. However, H. pylori has a really cool mechanism that allows it to transform its shape when it's under stress – examples of stress may include a change in pH or salinity, or an increase in the gases present – like nitrogen, carbon dioxide, and oxygen.    Christian: Uh huh…so what does it change its shape to?  Kimothy: Well, as I was saying a moment ago, H. pylori is hard to grow on a plate outside its ideal habitat.  It turns out that even in its ideal habitat, the stomach, the bacterium only thrives in these really specific conditions of pH and gas and any deviation from those conditions will cause the cells to become dormant and actually change shape. So check this out: H. pylori is spiral or helix-shaped (that's where the name comes from, Helico-bacter) and form follows function here, so it needs to burrow into the epithelium in the stomach lining in order to survive, so it uses its spiral shape and several flagella to literally corkscrew itself into our stomach to take residence. But, if the cell becomes stressed because a change in any of those conditions – pH, salt, gas, or temperature – it will slow down its metabolic machinery and change from its spiral shape and into a coccoid form.  It's still unclear if this transformation under stress is a selected adaptation or something else.  Several studies point to H. pylori's transformation as an evolutionary adaptation to cope with stress and others show no relationship.  There need to be more experiments to tease this out, but it is clear that a VBNC state is common with H. pylori, which again makes this a very, very difficult organism to culture from biopsies.  Christian: OK, we did get pretty deep there, let me just recap real quick: H. pylori is a gram-negative pathogen that is spiral (helix-shaped) with several flagella, which make it very motile; it is really selective about its environment (which is the stomach) and it doesn't grow well at all outside of that environment, in fact it usually enters a VBNC state if it becomes stressed in any way and when it's stress it changes shape into a coccoid form – this is a smaller spherical shape, right? I think the etymology of coccoid is actual berry in Greek, right? So, it transforms from a rod-spiral to a berry-shape? Lastly, and most importantly H. pylori results in the host ultimately getting stomach ulcers and even cancer, right?  OK, but we've been talking a lot about this pathogen living in stomachs, H. pylori is also in bulk water, though.   Kimothy: Yes, so the route of infection for H. pylori is still a bit mysterious and not always well-characterized to researchers. Contaminated water sources are certainly a means of infection, but so is fecal-oral and mucous-oral routes. This can result from living in close quarters with a large community and just not have access to proper disinfectants on touchable surfaces.  Once one person in a close-quarters household acquires H. pylori the R0 in that sample size will increase over time according to a Stanford Medical School manuscript in 2006.  R0 is just a term we use to describe the rate of infection to other individuals.    Christian: So the good news, if there is good news, is that educating people on transmission routes and increasing access to disinfectants can likely really disrupt or lower the R0.  Kimothy: That's right, Christian.  I'd like to come back to bulk water and liquid biopsies though if I may for a moment. Because H. pylori is so challenging to culture one of the best and most reliable ways to detect and study H. pylori is by using molecular diagnostic tools such as NGS using 16S rRNA and qPCR. We've talked about pathogens that cause pneumonia and acute respiratory infections, we've talked about pathogens that infect the blood, and now we have a pathogen that infects your gut with little to no indication of infection in most patients.  And although worldwide infection rates are going down, largely because of what you mentioned – access to clean water and surface disinfectants, the best way we can track and surveil this bug is by using these new diagnostic tools. Culturing for this bug is just too time consuming, unreliable, and not specific enough. It's analog in a digital world.  Christian: All right, well H. pylori…the peptic ulcer disease-causing gram-negative bacterium.  One more thing before we go – I know you're not a medical doctor, but how is H. pylori usually treated? Just a super dose of antibiotics?   Kimothy: Yeah, bacterium is very susceptible to antibiotic regimens, so it can usually be eradicated with a high-powered antibiotic.    Christian: Well, cool deal. We'll put some links in the show notes to some of these manuscripts we've mentioned in case you're interested. Kimothy, as always, thanks so much for the chat today.  Kimothy: You bet, Christian.

On this Culturing Creativity episode, we geeked out over games and discussed how different games may enhance your creativity, and can sharpen skills that are useful in your writing. Who knew that playing games could have such great perks?

In this episode of Lab to Startup, I speak with Brian Feth and James Lim, co-founders of XCellBio. We explore the startup story and learn about: The founding story- the initial hypothesis behind the product they wanted to build and how the technology evolved since then based on customer feedback Fundraising trials and tribulations How there was a lesson hidden in every “No” they received from potential customers and investors Role that partnerships played Traits like flexibility, open communication and co-founder chemistry Learn more about XCell Biosciences: https://www.xcellbio.com

On this culturing creativity episode, we discussed how where and when we write can effect the productivity, and—in some cases—the quality of our writing. This discussion included a very amusing breakdown of barriers we have found with writing both in cafes and outdoors. We talked about our day to day writing life, and incorporating writing into our vacations.

On this culturing creativity episode, we explored the benefits of cowriting, and discussed our experience as coauthors. We talked about the challenges involved, and how we overcome them.

This week for our culturing creativity episode, we discussed the effect clutter has on creativity: good, bad or neither? We talked about our personal habits, and whether we think these habits make us more or less productive.

In this culturing creativity episode of Dear Writer, we discussed the benefits of music in creating inspiration. This included both inspiration for plot events and character development within your book, but also in encouraging the muse to roll up his or her sleeves and get down to business!

In this culturing creativity episode, we explore procrastination. Why writers procrastinate, our own personal foibles and flaws, and what you can do to stop procrastinating and start writing.

We experimented with freefall (aka free writing) on Dear Writer this week, which proved an interesting and useful tool for developing creativity. Listen in to see how it went.

Welcome to the second episode of our miniseries, Culturing Creativity. Ever wondered what a typical day looks like for us? Listen in to find out. We have a chat about how we structure our days, and following that discuss how we manage to fit everything into our busy schedules. We don't always succeed. And, with that comes the acceptance that sometimes, you really can't do everything. But we do our best, every day, to culture the creativity in our lives.

Welcome to the first episode of our miniseries, Culturing Creativity. In this episode, we have a good chat about the way nature influences our writing, and techniques we use to nurture our creativity. Even when it's literally a blizzard outside, nature can still be a presence in our creative lives and inspire our writing. Listen in for tips on how you might include nature in your own life to grow your creativity.

Episode #20 of the Ground Shots Podcast. This episode of the Ground Shots Podcast was recorded at the Kaua'i Food Forest on a volunteer day one Saturday in May 2019. Freddie Mango Roots is one person in the core volunteer collective that tends the food forest on the island. He utilizes Korean Natural Farming techniques at the food forest as well as at his own gardens at home. We spend some time together going into Freddie's personal story, and venture into philosophy about our culture and the choices we make to work with the earth. We end the conversation with a recorded plant walk through the Kaua'i Food Forest.   In this conversation we also talk about: Freddie's stories of living in the wilderness for many years on the island of Kaua'i including a story about a helicopter crash more on plants and the food forest in addition to the sharing Paul Massey and Rob Cruz did on episode #17 being a product of your upbringing and a certain environment growing up in the deep south fermenting in the garden and kitchen how microbes are our ancestors Korean / Hawai'ian natural farming ferments the importance of cultural and ecological diversity and symbiosis the lessons of the garden for life – accepting constant change and shifting   Make sure to check out the photo diary of the Kaua'i Food Forest on the project blog page to see what some of the plants look like that Freddie mentions on the audio plant walk. Links: Freddie Mango Roots on instagram: @f.m.roots Kaua'i Food Forest facebook page Kaua'i Food Forest's website: http://www.kauaifoodforest.org   View the Kaua'i Food Forest hashtag: #kauaifoodforest     Support the podcast on Patreon to contribute to our grassroots self-funding of this project.    Our Instagram page @goldenberries Join the Ground Shots Podcast Facebook Group to discuss the episodes Subscribe to our newsletter for updates on the Ground Shots Project Theme music: 'Sweat and Splinters' by Mother Marrow Additional Music: "Pepeiao Cabin' by Lisa Schonberg and Secret Drum Band, of the Hylaeus Project Check out Secret Drum Band's music here Check out the Hylaeus Project here, a creative study of the endangered native Hawai'ian Hylaeus bees  Produced by: Opia Creative    

In today's episode, Dr Nirala Jacobi welcomes back Dr Jason Hawrelak, to talk about Microbiome Restoration. Dr Hawrelak is a researcher, educator, naturopath, and nutritionist with over 16 years of clinical experience.  He also practices at Gould's Natural Medicine - a 135-year-old natural medicine apothecary and clinic in Hobart, Tasmania, Australia. Dr Hawrelak completed his PhD examining the capacity of probiotics, prebiotics, and herbal medicines to modify the gastrointestinal tract microbiota.  He is the senior lecturer in Complementary and Alternative Medicines at the University of Tasmania's School of Medicine where he coordinates the evidence-based complementary medicines programs.  Dr Hawrelak also teaches the gastrointestinal imbalances unit, within the Masters of Science and Human Nutrition, and Functional Medicine Program at the University of Western States, in Portland, Oregon.   Topics discussed include: The SIBO Doctor courses Microbiome Restoration - coming soon Regrowing vs killing in the microbiome. Colonic collateral when we are dealing with SIBO, and the necessity to restore the colonic ecosystem as part of the SIBO treatment. How do we learn to interpret the DNA readings and PCR reports? The SIBO Doctor Microbiome Restoration course A discussion of the modules offered in the Microbiome Restoration course Module 1 - Testing The difference between different labs Culturing vs DNA techniques Assessment, Interpretation, and Diagnosis Module 2 - Beneficial Bacteria, including Akkermansia Faecalibacterium prausnitzii Butyrate-producing microbes Hydrogen sulfide gas producers Module 3 - Pathobionts The good, the bad, the ugly Hydrogen sulfide producers Methanobrevibacter smithii coli Bacteroides Triple antibiotic therapy (for conditions such as H. Pylori and Blastocystis Hominis) and the impact on the colonic ecosystem balance of symbionts and pathobionts. Dietary changes vs probiotic supplements to elicit change in the gut microbiome. The complications between advising prebiotic nutrition for optimising microbiome restoration, whilst clients are on therapeutic diets such as the Low FODMAP diet - how to reconcile? Hydrogen sulfide breath testing - coming soon. High-fat content diets in SIBO, and how this can eventually feed hydrogen sulfide producing bacteria. Symptoms such as food reactivity and visceral sensitivity as a consequence of dietary choices in SIBO treatment, in regards to hydrogen sulfide producing bacterial blooms. The therapeutic benefits of Soy Isoflavones in relation to Methane and Hydrogen Sulfide producing SIBO patients. Equol producers - patients who have bacteria that convert soy isoflavones into the bioactive component, Equol. Adlercreutzia levels in people who eat soy products as a long-term dietary choice. Is there a connection between histamine intolerance, salicylate intolerance, and oxalate intolerance and microbiome disturbances? Post SIBO treatment food sensitivities - the colonic microflora patterns to observe. Hydrogen sulfide gas and how it causes visceral sensitivity, gut leakiness, and inflammation in the nerves. Dietary changes with people with sensitivities - a reiteration of start low, go slow. Is there any clinical significance to oxalobacter formigenes being elevated? What is the dietary impact of the extinction of oxalobacter formigenes? Testing levels of Proteobacteria to uncover the innate endotoxin load (proteobacteria - gram-negative bacteria that secrete proinflammatory endotoxins). The systemic repercussions of endotoxemia - leaky blood-brain barrier, gut damage, insulin sensitivity impacts, systemic inflammation, Alzheimer's, anxiety and depression links. Endotoxin (also known as Lipopolysaccharides - LPS) absorption and high-fat How do different Phyla respond to different diets? Potential outcomes of the ketogenic diet depending on the dietary nuances - decreased microflora diversity, increased hydrogen sulfide gas-producing bacteria, and increased numbers of proteobacteria. Akkermansia and constipation - why are these often seen together? Gut inflammation and mucus tend to increase Akkermansia if it is there - it is a mucin eater. Gut markers of inflammation, such as calprotectin. The vocabulary to use around probiotics - not re-seeding, but rather restoring. The importance of lactic and acetic acid production adjusting the pH of our microbiome to be hospitable to beneficial bacteria. D-Lactate considerations. Biogaia effectively reducing methane production, and also being used alongside proton pump inhibitors (PPIs) to prevent the development of SIBO. PPIs - when to use and when not to - listen to Dr Steven Sandberg-Lewis' podcast on SIBO and Functional GIT Exam Skills. Plantarum LP8 is being used in research to decrease Desulfovibrio, increase butyrate and bifidobacterium - can we use other strains of L. Plantarum to elicit similar changes? Plantarum strains to use in gut rehabilitation. Reuterin production. Dr Hawrelak's antimicrobial and dietary recommendations for treating Desulfovibrio.   Resources Dr Hawrelak Probiotic Advisor Gould's Natural Medicine   The SIBO Doctor Microbiome Restoration Course with Dr Jason Hawrelak [Opens 13 June] Sign Up for the Course and Live Q & A with Dr Hawrelak The SIBO Conference New Orleans - speakers mentioned Dr Richard McCallum Dr Matthew Bohm   Labs mentioned Ubiome - microbiome DNA testing Genova   Dr Satish Rao SIBO and comorbid SIFO D-Lactic Acidosis researcher   Dr Steven Sandberg-Lewis Dr Steven Sandberg-Lewis' podcast on SIBO and Functional GIT Exam Skills