Class of molecules found in Gram-negative bacteria
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11 episodes with endotoxin
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2 episodes with endotoxin
3 episodes with endotoxin
2 episodes with endotoxin
This Real Science Exchange podcast episode was recorded during a webinar from Balchem's Real Science Lecture Series. You can find it at balchem.com/realscience.Feeding rumen-protected choline in early lactation has consistently increased milk yield and energy-corrected milk yield, which is more pronounced when cows are fed diets low in metabolizable methionine. Choline feeding also increases milk fat and protein yield, minimizes body condition loss in early lactation, and reduces postpartum disease incidence. Dr. McFadden presents three topics about choline biology in the dairy cow. (01:45)Why should we consider fatty acid feeding when feeding methyl donors like choline and methionine?Choline degradation in the rumen and small intestine, focusing on the role of triethylamine oxide Why should we consider lysophosphatidylcholine as an immunomodulator in fresh cows and preweaning calves?Fatty acid nutrition to optimize methyl donor efficiency. (4:02)Fatty liver is a concern for fresh cows because of its relationship with ketosis, poor fertility and compromised milk production. Cows with fatty liver exhibit low circulating concentrations of phosphatidylcholine, which is a component of very low-density lipoproteins (VLDL) that transport triglycerides out of the liver. Feeding rumen-protected choline lowers liver triglyceride deposition by supporting the synthesis of phosphatidylcholine and thus, VLDL. Dr. McFadden goes on to explain the two different pathways for phosphatidylcholine in the liver and how those interact with fatty acid metabolism. He describes several experiments that have investigated how rumen-protected choline and supplemental fatty acids interact in lactating cows. Low phosphatidylcholine supply is a key feature of fatty liver in dairy cows, likely due to low polyunsaturated fatty acid (PUFA) and low choline supplies. Delivery of post-ruminal PUFA may support phosphatidylcholine synthesis with accompanying improvements in insulin sensitivity, body condition maintenance, and inflammation, but interactions with dietary fatty acid digestibility should be considered. Dr. McFadden gives a list of considerations for fresh cow diets incorporating fat and choline supplementation. Gastrointestinal choline degradation and trimethylamine N-oxide (TMAO) (16:58)Unprotected choline is almost totally degraded in the rumen. Microbes convert choline into trimethylamine (TMA) which is then converted to TMAO in the liver. Rumen-protected choline allows for a large proportion of choline to reach the small intestine intact. However, research shows that choline can also be degraded by microbes in the small intestine in the same pathway, limiting choline bioavailability. Plasma TMAO accumulation is associated with non-alcoholic fatty liver disease, inflammation, insulin resistance, obesity, oxidative stress, and cardiovascular disease in rodent and human models. Little research was available regarding if the relationship between TMAO and poor health was causative or just associative. Dr. McFadden's lab infused cows intravenously with TMAO and found that TMAO did not modify milk production or glucose tolerance in early lactation cows. TMAO does not appear to influence energy metabolism or health in early lactation cows. Choline is subject to both ruminal and lower-gut degradation to TMA, and that influence on choline bioavailability needs to be defined. Data in non-ruminants suggests that unsaturated fatty acid feeding can shift the gut microbes to slow TMA formation. Lysophosphatidylcholine and immunomodulation (28:45)Dr. McFadden gives an overview of neutrophil activation and the oxidative burst that contributes to pathogen killing. The ability to elicit the oxidative burst is diminished in pre-weaned calves and transition cows. When cows were given endotoxin to cause an immune response, circulating lysophosphatidylcholine was decreased. In rodent models, lysophosphatidylcholine promotes the oxidative burst and suppresses long-term inflammation in response to bacterial infection. Dr. McFadden cultured neutrophils from pre-weaned calves with lysophosphatidylcholine and observed an enhanced oxidative burst.Immunosuppression is characterized by low circulating lysophosphatidylcholine concentrations in dairy cows. In vitro data suggests lysophosphatidylcholine can activate neutrophils, and rumen-protected choline increases circulating lysophosphatidylcholine. Future research is likely to define an immunomodulatory role for choline. Dr. McFadden takes questions from the webinar audience. (38:07)Please subscribe and share with your industry friends to invite more people to join us at the Real Science Exchange virtual pub table. If you want one of our Real Science Exchange t-shirts, screenshot your rating, review, or subscription, and email a picture to anh.marketing@balchem.com. Include your size and mailing address, and we'll mail you a shirt.
For more information, visit https://thecirsgroup.com The GENIE test is a blood test you can take to help diagnose CIRS, or Chronic Inflammatory Response Syndrome, but it can also inform your treatment! Today we have our favorite guest, Dr. Christian Navarro-Torres, back to explain what the GENIE test is good for, what you might do differently based on your results, and how you can learn about your own with the help of a coach or doctor. For more information, support, and resources in your own CIRS healing journey, visit TheCIRSGroup.com TIME STAMPS: 0:00 Intro and disclaimer 5:00 What is the GENIE test? 7:30 What does gene activity mean in the context of CIRS? 9:00 HLA Haplotype test vs GENIE 11:20 What are the benefits of getting the GENIE test done? 16:35 Examples of genetic expressions and what they mean 19:57 Indications of Lyme 20:30 Downregulated Metabolism 24:00 Endotoxin hyperreactivity 26:30 Get help to understand your own GENIE test 28:57 How to work with Christian HELPFUL LINKS: Christian's membership communities, both for patients and practitioners: https://www.cirslab.com/ Work with Christian: https://www.cirslab.com/contact Our previous episode on the GENIE: https://youtu.be/bCNp9qNqWWc?si=BqrZHUthROdyiiU5 Check out The CIRS Summit! http://thecirssummit.com/ Our previous episodes with Dr. Christian Navarro-Torres: Neuroquant: https://youtu.be/aQyU51D7O-c?si=-hol5adPP5SLsNN5 ADHD: https://youtu.be/x_yL-voDyJY?si=48kzS4gRVZi0u8BM Nicotine: https://youtu.be/hLumLIye7q4?si=JXp_Y7vozJxkS7_e The CIRS Group: Support Community: https://thecirsgroup.com Instagram: https://www.instagram.com/thecirsgroup/ Find Jacie for carnivore, lifestyle and limbic resources: Instagram: https://www.instagram.com/ladycarnivory YouTube: https://www.youtube.com/@LadyCarnivory Blog: https://www.ladycarnivory.com/ Pre-order Jacie's book! https://a.co/d/8ZKCqz0 Find Barbara for business/finance tips and coaching: Website: https://www.actlikebarbara.com/ Instagram: https://www.instagram.com/actlikebarbara/ YouTube: https://www.youtube.com/@actlikebarbara Jacie is a Shoemaker certified Proficiency Partner, NASM certified nutrition coach, author, and carnivore recipe developer determined to share the life changing information of carnivore and CIRS to anyone who will listen. Barbara is a business and fitness coach, CIRS and ADHD advocate, speaker, and a big fan of health and freedom. Together, they co-founded The CIRS Group, an online support community to help people that are struggling with their CIRS diagnosis and treatment.
The horseshoe crab has endured for over 450 million years. It has survived several mass extinctions including the one that killed off the dinosaurs. One reason for their incredible resiliency is their ability to fend off bacterial infection. Their blood contains cells that clot around invading bacteria, thereby protecting them from the attacking toxins.In this episode we talk with three experts about how this animal's unique blue blood has become essential to modern medicine. We also talk about why horseshoe crab populations are dwindling, and what biotech is doing to address the shortfall.Follow us on LinkedIn, X, Facebook and Instagram. Visit us at https://www.bio.org/
Free Energy Balance Food Guide: https://jayfeldmanwellness.com/guide The Nutrition Blueprint: https://mikefave.com/the-nutrition-blueprint/ Danny's Telegram: https://t.me/dannyroddy Danny's Substack: https://dannyroddy.substack.com/ Timestamps: 0:00 – intro 0:36 – catching up: fruit quality in the Philippines and Ecuador, and the effects of mold toxicity 5:28 – Kiran Krishnan and Nutrition with Judy discuss why they believe coconut oil should not be consumed 9:55 – whether coconut oil causes greater release and absorption of endotoxin (LPS) 12:11 – should you eat coconut oil even if your ancestors didn't? 21:08 – how MCT oil compares to coconut oil as it relates to the microbiome 32:28 – concerns with adopting dietary ideologies 36:21 – Morley Robbins on why the thyroid doesn't run the body and we shouldn't ignore the adrenals 47:05 – adrenal fatigue as a systemic issue and whether the thyroid or adrenals run the body 56:45 – our take on Real Food Gangsta's (Josh & Jeanne Rubin) view that the adrenal glands regulate the body's mineral and fuel availability 1:07:02 – the importance of optimal gut motility and how to alleviate gut motility issues such as constipation and loose stools 1:19:54 – which lab tests provide the most valuable information 1:25:14 – the benefits of spending time without stimulation (letting your mind wander) during the day 1:27:20 – simple mindset strategies to help you reach your goals 1:32:55 – Alan Aragon's argument that not all seed oils are harmful 1:40:56 – why the claim that seed oils lower cholesterol does not mean they are healthy 1:42:25 – whether animal studies are valuable for understanding the effects of seed oils on human health
Why You Should Listen: In this episode, you will learn about integrative pediatrics and how to create whole child resilience. About My Guest: My guest for this episode is Dr. Elisa Song. Elisa Song, MD is a Stanford, NYU, UCSF-trained integrative pediatrician, pediatric functional medicine expert, and mom to 2 thriving children - and she is on a mission to revolutionize the future of children's health. Dr. Song is the bestselling author of the "Healthy Kids Happy Kids: An Integrative Pediatrician's Guide to Whole Child Resilience". Dr. Song created Healthy Kids Happy Kids as an online holistic pediatric resource to help practitioners and parents bridge the gap between conventional and integrative pediatrics with an evidence-based, pediatrician-backed, mom-approved approach. In her integrative pediatric practice, she's helped 1000s of kids get to the root causes of their health concerns and empowered parents to help their children thrive by integrating conventional pediatrics with functional medicine, homeopathy, acupuncture, herbal medicine, and essential oils. Dr. Song is chair of A4M's pediatric education and has lectured around the world at leading integrative and functional medicine conferences and premier parenting events. She has also been featured in hundreds of top podcasts, print and online media outlets, including the Wellness Mama podcast, BloomTV, Forbes, Healthline, MindBodyGreen, National Geographic, PopSugar, Parents, Motherly, Parade, Verywell Health, and New York Post. Key Takeaways: Can Ulcerative Colitis and Crohn's be resolved? What role do environmental toxicants play in children's health? What are the more common patterns of GI dysbiosis? Can the fingerprint of the microbiome paint the diagnosis? When does neurotransmitter testing make sense? How important is exploring SNPs in children? What are some tools to address intestinal hyperpermeability? What is the role of autoimmunity and immune modulation in persistent conditions? What increases and decreases endotoxins or LPS in the body? Is it more important to add more good guys or to reduce the bad guys? Is kombucha a health food? How do artificial food dyes and colorings impact the microbiome? Are all seed oils bad? Which artificial sweeteners support our microbiome and which harm it? How does glyphosate impact health? Is EMF hygiene in the sleep location important? Are prebiotics feeing the bad guys as much as the good guys? What are the best tools for evaluating and treating parasites? Connect With My Guest: http://HealthyKidsHappyKids.com Related Resources: Book: Healthy Kids, Happy Kids Interview Date: August 1, 2024 Transcript: To review a transcript of this show, visit https://BetterHealthGuy.com/Episode204. Additional Information: To learn more, visit https://BetterHealthGuy.com. Disclaimer: The content of this show is for informational purposes only and is not intended to diagnose, treat, or cure any illness or medical condition. Nothing in today's discussion is meant to serve as medical advice or as information to facilitate self-treatment. As always, please discuss any potential health-related decisions with your own personal medical authority.
For more information, visit https://thecirsgroup.com CIRS, or Chronic Inflammatory Response Syndrome, is an illness that can affect up to 25% of the population, thanks to genetics and environmental toxins. Today, we continue to recap CIRSx 2024, this time showing our Indoor Environmental Professionals some love! We had some fantastic talks by Michael Schrantz, Bill Weber, and Brandon Apple, plus Dr. Eric Dorninger and Dr. Jari Louhelainen and the topics covered include poop (or endotoxins), indoor air quality, indoor air testing, and the proper sequencing of remediation. For more information, support, and resources in your own CIRS healing journey, visit TheCIRSGroup.com TIME STAMPS: 0:00 Intro and disclaimer 1:13 Endotoxin talk by M. Schrantz and Dr. Dorninger 2:15 Endotoxins don't always smell 2:58 How to test for endotoxins 4:39 DIY tips to deal with Endotoxins 6:30 What to avoid to keep endotoxins at bay 7:29 Air Quality talk by Dr. Jari Louhelainen 9:57 Real time air testing 11:00 The problem with HEPA filtration 11:57 Prevalence of government and leadership cover up 13:44 Mold Sampling Methods talk by Bill Weber 15:00 Air Sampling pros and cons 16:50 When to use air sampling 17:53 Why hire your own IEP or inspector 19:21 Remediation Sequencing talk by Brandon Apple 21:54 Verify after every step - test, test, test 24:00 Lean into your CIRS-literate IEP 25:15 The painful verification process The CIRS Group: Support Community: https://thecirsgroup.com Instagram: https://www.instagram.com/thecirsgroup/ Find Jacie for carnivore, lifestyle and limbic resources: Instagram: https://www.instagram.com/ladycarnivory YouTube: https://www.youtube.com/@LadyCarnivory Blog: https://www.ladycarnivory.com/ Limbic Training Community: https://www.wired4healing.com Find Barbara for business/finance tips and coaching: Website: https://www.actlikebarbara.com/ Instagram: https://www.instagram.com/actlikebarbara/ YouTube: https://www.youtube.com/@actlikebarbara HELPFUL LINKS: Michael Schrantz' company website: https://environmentalanalytics.net/ Dr. Doringer's website: https://drdorninger.com/ Dr. Jari Louhelainen: https://www.ljmu.ac.uk/about-us/staff-profiles/faculty-of-science/pharmacy-and-biomolecular-sciences/jari-louhelainen Bill Weber's company website: https://avelar.net/articles_publications/team/bill-weber/ Brandon Apple's company website: https://www.moldsci.com/ Endotoxin test: https://www.envirobiomics.com/product/e-endotoxin/?v=3e8d115eb4b3 National Company that administers smoke tests: https://www.americanleakdetection.com/ Green Gobbler product: https://a.co/d/9jTf8vW ERMI Test: https://www.envirobiomics.com/product/ermi/?v=3e8d115eb4b3 HERTSMI-2 Tests: https://www.envirobiomics.com/product/hertsmi-2/?v=3e8d115eb4b3 Jacie is a 4 year carnivore, certified nutrition coach, and carnivore recipe developer determined to share the life changing information of carnivore and CIRS to anyone who will listen. Barbara is a coach, facilitator, speaker, 3 year carnivore, and a big fan of health and freedom. Together, they co-founded The CIRS Group, an online support community to help people that are struggling with their CIRS diagnosis and treatment.
For more information, visit https://thecirsgroup.com CIRS, or Chronic Inflammatory Response Syndrome, is an illness caused by mold and other biotoxins that affects 24% of the population and only a handful of doctors even know about it. We are so excited to have one of the greats on today: Dr. Eric Dorninger. Dr. D is a CIRS patient himself - he will share more about his and his family's health struggles, the reasons he fights for his patients' health, and the magic of healing from CIRS so we can be happy, healthy, kick ass individuals. For more information, support, and resources in your own CIRS healing journey, visit TheCIRSGroup.com TIME STAMPS: 0:00 Intro and disclaimer 1:18 Dr. Eric Dorninger's backstory 8:35 CIRSx and the Holy Trinity of CIRS Healing 9:18 BlueSkyCBD for mood disorders 10:10 Screening for CIRS 13:04 Story of patient healing from CIRS 14:59 Why your doctor's staff is so important 20:50 What it takes to work with CIRS patients 23:41 Tinnitus in CIRS patients 24:54 The problem with naming diseases 26:30 Dr. D's podcast The Vitality Hour 29:44 Patient Aaron Pettigrew's CIRS story 32:39 CIRSx and the importance of environment professionals 37:00 CIRS patients becoming CIRS practitioners 39:37 What the CIRS community should do to spread awareness 41:57 The problem with urinary mycotoxin tests and azoles 44:20 Endotoxin nuggets of wisdom 49:09 Outro and CIRSx excitement HELPFUL LINKS: Come to CIRSx! https://www.cirsx.com/ Dr. Dorninger's practice website: https://drdorninger.com/ Dr. Dorninger on AANP website: https://naturopathic.org/member/drdorninger BlueSkyCBD: https://www.bluesky-cbd.com/ Dr. D's podcast The Vitality Hour: https://www.youtube.com/channel/UCwmPARlXz7FIag_6NPS_uBg Vitality Hour on tolerating binders: https://youtu.be/omQL_mZrGRM?si=5DtyoM8E4i9I4qfU Take the CIRSx Courses: https://institute.cirsx.com/ The CIRS Group: Support Community: https://thecirsgroup.com Instagram: https://www.instagram.com/thecirsgroup/ Find Jacie for carnivore, lifestyle and limbic resources: Instagram: https://www.instagram.com/ladycarnivory YouTube: https://www.youtube.com/@LadyCarnivory Blog: https://www.ladycarnivory.com/ Limbic Training Community: https://www.wired4healing.com Find Barbara for business/finance tips and coaching: Website: https://www.actlikebarbara.com/ Instagram: https://www.instagram.com/actlikebarbara/ YouTube: https://www.youtube.com/@actlikebarbara Jacie is a 4 year carnivore, certified nutrition coach, and carnivore recipe developer determined to share the life changing information of carnivore and CIRS to anyone who will listen. Barbara is a coach, facilitator, speaker, 3 year carnivore, and a big fan of health and freedom. Together, they co-founded The CIRS Group, an online support community to help people that are struggling with their CIRS diagnosis and treatment. Catch Jacie and Barbara on Judy Cho's podcast to learn more about their health journeys and why they started The CIRS Group: When Carnivores are Affected by Mold Illness - Jacie Gregory & Barbara Williams - https://youtu.be/CR8Uj-d_fok
Norm Wainwright and Foster Jordan have been immersed in the field of limulus amebocyte lysate (LAL) testing for decades, and have seen the progress and refinement of this crucial safety test. With the 20th anniversary of Charles River's Endosafe Cartridge and the recent introduction of recombinant alternatives, we look back at the past and future of endotoxin testing.
Kevin McKernan is the Chief Scientific Officer and Founder of Medicinal Genomics. Kevin has pioneered the genomics of cannabis and hemp to build a stronger scientific environment for the study of cannabis based therapeutics and blockchain technologies for tracking and verifying cannabis genetics. At CannMed 24 he will present “Understanding the Prevalence and Risks Associated with Endotoxins on Cannabis Flower”, which will include novel research he did to measure the level of endotoxins in store-bought cannabis flower. Our conversation covers: What inspired Kevin to look into endotoxin levels on cannabis Which microbes produce endotoxins How endotoxins affect humans Whether remediation techniques mask endotoxin risks and more Thanks to This Episode's Sponsor: Agilent Technologies. Whether you manufacture or test cannabis, you face the challenge of delivering high-quality products, increasing sample volume, and meeting emerging regulations. Agilent offers best-in-class cannabis and hemp analysis solutions featuring robust instruments, software, services, and consumables. Their team of experts can develop, implement, and optimize methods to get you up and running quickly. Learn more at agilent.com Additional Resources High levels of endotoxins in commercial cannabis flower [Pre-Print] Total Yeast and Mold - A Comparison Study - Jini Glaros [CannMed 23 Presentation] What Makes CannMed Unique and Why You Don't Want to Miss It Register for CannMed 24 Review the Podcast! CannMed Archive
I read from endoscopic to endotoxin. Endosperm Nucleus https://en.wikipedia.org/wiki/Endosperm "In the United States, endosulfan is only registered for agricultural use, and these uses are being phased out." https://en.wikipedia.org/wiki/Endosulfan The word of the episode is "endoskeleton". https://en.wikipedia.org/wiki/Endoskeleton Theme music from Tom Maslowski https://zestysol.com/ Merchandising! https://www.teepublic.com/user/spejampar "The Dictionary - Letter A" on YouTube "The Dictionary - Letter B" on YouTube "The Dictionary - Letter C" on YouTube "The Dictionary - Letter D" on YouTube "The Dictionary - Letter E" on YouTube Featured in a Top 10 Dictionary Podcasts list! https://blog.feedspot.com/dictionary_podcasts/ Backwards Talking on YouTube: https://www.youtube.com/playlist?list=PLmIujMwEDbgZUexyR90jaTEEVmAYcCzuq https://linktr.ee/spejampar dictionarypod@gmail.com https://www.facebook.com/thedictionarypod/ https://www.threads.net/@dictionarypod https://twitter.com/dictionarypod https://www.instagram.com/dictionarypod/ https://www.patreon.com/spejampar https://www.tiktok.com/@spejampar 917-727-5757
As we visit the third and final "Top Episode of 2023", we will turn our attention to our relatively new best red light therapy friend: methylene blue! For those of you that have already heard this episode and/or are familiar with this polarizing substance, this episode is a fantastic review; for those of you that are less familiar, this is a great introduction into the vast potential of methylene blue.... Let's get blue on today's solosode of The Red Light Report! The last couple of weeks you have likely heard and/or learned about BioLight's newest innovative product, BioBlue, which is an enhanced methylene blue and mitochondrial supplement. Some of you are even using it right now! But I felt it would behoove all of us to take a deeper dive into this mysterious synthetic textile dye that happens to seemingly be one of the most profound health, wellness and mitochondrial-boosting substances available. And it is a major synergist when combined with red light therapy! So today, I thought it would be great to review the top 10 uses for methylene blue, as professed by scientific research. And while I don't want to give away too much in this intro, I feel obliged to tell you a few of the things that I find most exciting and promising about methylene blue:1.) Secondary to its ease of crossing the blood-brain barrier, a vast majority of the methylene blue consumed concentrates in the brain, which is why it has virtually endless brain health benefits (i.e., reduced symptoms of Alzheimer's, Parkinson's, dementia, depression, PTSD and improved cognition, mood and energy);2.) Methylene blue has an innate intelligence to help whichever cells are most sick, diseased and/or cancerous first before moving along and helping other healthier cells;3.) There are only two known interventions capable dissociating nitric oxide from the cytochrome c oxidase (which then allows oxygen into the mitochondria for more efficient energy production; remember, the more energy in a cell = less disease state/more vitality in cell): red light therapy and methylene blue. Remember, none of this information is a substitute for medical advice. But just like when I learned about red light therapy for the first time and was in awe at its endless health benefits, I am equally impressed by the limitless potential of methylene blue. Both are safe, efficacious and likely have a pivotal role to play for anyone looking to optimize their health and longevity. As always, (red) light up your (blue) health! - BioBlue, BioLight's newest product: (0:00) Diving into methylene blue: (7:30) Malaria cured in 48 hours: (10:48) Brain function and methylene blue: (12:29) Metabolism and methylene blue: (21:07) Top ten benefits of methylene blue: (21:56) Combining methylene blue and red light therapy: (29:16) COVID-19 and methylene blue: (30:03) Alzheimer's and Parkinson's and methylene blue: (33:30) Dosages: (38:48) Hallmarks of Alzheimer's and methylene blue: (39:54) Metabolic hallmarks: (41:04) Red light therapy and methylene blue for dimensia: (43:11) Cognitive enhancement: (45:23) Depression: (48:56) Autism: (54:41) Endotoxin in the gut: (1:01:02) Pain relief: (1:03:49) The heart: (1:09:53) Cancer: (1:11:48) Red light therapy and methylene blue: (1:14:07) Methylene blue therapy for cancer: (1:16:14) - Book referenced from the episode: The Ultimate Guide to Methylene Blue, by Mark Sloan - Exclusive 15% discount on BioBlue for The Red Light Report listeners! Use discount code: bioblue15 - Kindle version of Red Light Therapy Treatment Protocols eBook, 4th Edition - To learn more about red light therapy and shop for the highest-quality red light therapy products, visit https://www.biolight.shop - Dr. Mike's #1 recommendations: Grounding products: Earthing.com EMF-mitigating products: Somavedic Blue light blocking glasses: Ra Optics - Stay up-to-date on social media: Dr. Mike Belkowski: Instagram LinkedIn BioLight: Instagram YouTube Facebook
The horseshoe crab has endured for over 450 million years. It has survived several mass extinctions including the one that killed off the dinosaurs. One reason for their incredible resiliency is their ability to fend off bacterial infection. Their blood contains cells that clot around invading bacteria, thereby protecting them from the attacking toxins.In this episode we talk with three experts about how this animal's unique blue blood has become essential to modern medicine. We also talk about why horseshoe crab populations are dwindling, and what biotech is doing to address the shortfall.Follow us on LinkedIn, X, Facebook and Instagram. Visit us at https://www.bio.org/
Show Notes and Transcript Robert Malone's Substack has become essential reading for many of us. Every day nuggets of wisdom and insight, information and humour drop into inboxes all over the world. One of the recent emails caught our eye. What is Woke? It's a word that we have come familiar with but many of us would struggle to define it. It is an intolerant ideology that is sowing seeds of confusion and division. Robert returns once again to Hearts of Oak to discuss what sparked the article and we have a close look at 15 examples of wokeness that he has referenced before we touch on another of his Substacks about 'Adulteration', which is a topic that we hope to come back to in more detail soon. Robert W Malone MD, MS Inventor of mRNA & DNA vaccines, RNA as a drug. Scientist, physician, writer, podcaster, commentator and advocate. Believer in our fundamental freedom of free speech. Connect with Dr Malone..... GETTR: https://gettr.com/user/rwmalonemd X: https://twitter.com/RWMaloneMD?s=20 WEBSITE: https://www.rwmalonemd.com/ https://maloneinstitute.org/ SUBSTACK: https://rwmalonemd.substack.com/ Interview recorded 26.10.23 *Special thanks to Bosch Fawstin for recording our intro/outro on this podcast. Check out his art https://theboschfawstinstore.blogspot.com/ and follow him on GETTR https://gettr.com/user/BoschFawstin and Twitter https://twitter.com/TheBoschFawstin?s=20 To sign up for our weekly email, find our social media, podcasts, video, livestreaming platforms and more... https://heartsofoak.org/connect/ Support Hearts of Oak by purchasing one of our fancy T-Shirts.... https://heartsofoak.org/shop/ Transcript (Hearts of Oak) Robert W. Malone, MD. It is wonderful to have you back. Thanks so much for joining us today, Robert. (Robert Malone MD) Peter, thanks for your friendship. It's been a pleasure to host you on the farm a couple of times and all of our many podcasts. I don't know how many we're up to right now, but I always enjoy talking to you and through your to your audience. So thanks for having me on again. No, thank you. And if what you're doing doesn't work out, if everyone deserts you on Substack, then I think you do have another future, which is steaks. I think that was the best steak I've had outside Texas. So I say, open up a steak restaurant, Robert. Yeah, that might be my future if the powers that be have their way with me. If you say too much, because that's just safer. But just for the viewers, @rwmallonemd on Twitter, on GETTR, anywhere else, And of course, Substack, rwmalonemd.substack.com. You just type in Substack, Robert Malone will come up. All the links are in the description. And that is also a way to support what Robert does in the many places he travels, speaks, interviews, and all of that. That is a one way of supporting him. or getting his book, which I had beside me, Lies My Government Told Me. There it is, Lies My Government Told Me. Make sure if you haven't got a copy of that, get a copy, it's a great Christmas present. About to come out in Norwegian for all of your Norwegian listeners and in Dutch. I think we're gonna have the book launch in Norway in early December and the book launch in the Netherlands sometime in February. So stay tuned for that. And then we have another book that is just nearing completion on Cy-War and Sovereignty, which is the big propaganda, you know, there in the UK, you're certainly familiar with nudge technology and the 77th Brigade and MI6 and all of their various nefarious activities through the COVID crisis. Oh, yeah. I look forward to that coming out. The Norwegian languages, maybe I'll give it a miss. So I'll wait for the new book. We've got a great cover. I look forward to it. Robert, I love your Substack. There may be four different sub-stacks that I follow because you can have an avalanche of information and yours is one of those. And my favorite thing is you don't know what you're going to get. Each day is different. It could be on the farm, it could be travel, it could be latest scientific research coming out, it could be government agencies and how they work. And I love that huge mix and that wide array. And one of the recent ones just days ago was, what is woke and intolerant and moralizing ideology? And you had a little cartoon at the beginning and at the end, the definition was stuff I don't like, which I actually thought was a good definition. But it's a term that I think we now use frequently and we kind of know how to describe things as woke, but we're not very sure what exactly that is. If we see something, oh, that's woke, why? The definition of pornography, I can't define it but I know it when I see it. Same with woke. And yeah, so thanks. This was almost kind of a throwaway Substack, that Jill put together on the fly. Basically, we tend to wake up in the morning or as we're going to sleep the night before. This is our lives. Hey, welcome to our lives. We lay in bed and talk between the two of us. Well, when are we gonna write tomorrow? I don't know. Good heavens. And so, in this case, what transpired was we, when we like to turn off our brains, we turn to the, you know, streaming. We never watch television per se, but we stream. And they tend to fall into two categories, basically, science fiction and documentaries about history or travel. That's kind of our lives. I know it's pretty exciting. And so we were watching this new Netflix series called Bodies. And it proceeds because it's a kind of a multi-timeline thing that is all wrapped around the UK and London is the setting for the series. And I don't want to go into the plot line, but basically, in episode three, suddenly we have the insertion of this gratuitous, let's say, gently man love, with a fairly explicit scenery associated with it. And it was absolutely gratuitous, not necessary for the plot line. It was clearly another woke agenda insertion, as we have come to expect from Netflix routinely. And this one was so in your face that we just, both of us, looked at each other and flipped off the TV. Okay, enough of that. Time to go to bed. And then Jill, in the morning, woke up and she said, I've got to find some way to be able to pre-screen, these various streaming broadcasts for their woke content. I wonder if there's anything out there on the internet. And so she started searching, and she found this delicious site that is mentioned in the article. Notwokeshows.com, which I've delved into. Yeah, so she finds notwokeshows.com, and she's like, hallelujah, this is a goldmine, because they have listed all these criteria that they apply to define whether or not a show is woke, and whether or not they're going to include it in their recommended broadcast that one who is, let's say, not enjoying the woke agenda can safely view. I guess this is akin to the censorship board there that the BBC sponsors for you lucky members of the United Kingdom. And so you're all so fortunate that the government looks out for you so carefully and maintains your mental health in alignment with their interests. So in any case, she finds this website and it's such a rich repository of, you know, a little bit tongue in cheek, but on point. Commentary about what constitutes woke from the perspective of some group that is seeking to delineate woke from non-woke, broadcasting and being able to to list, non-woke content, that she writes to the authors and they write back and because we've become quite sensitized to issues concerning copyright. And so they write back and they say, yes, absolutely. You're free to use this. We're so grateful and all happy, happy. And so she takes basically their content and disambiguates or redacts it or restructures it so that it's not specifically about programming, but speaking more directly about what is WOKE and from the framework of these individuals that put together this website that previously has been relatively unknown and blasted out together with the crosslink to the source material, et cetera. And that's what gives rise to this fun little throwaway essay, which was her basically, uh-oh, we've got to get something out this morning. How about if we do this? while I happen to be writing an in-depth technical piece about adulteration and the RNA vaccines, which was going to take much longer. So we wanted to get something out to our Substack subscribers so they have it with their morning coffee. And that's the genesis of this particular essay. And I will finish on adulteration, another word that we've come across only in the last few days. But I love this article simply because it's what people are facing, the people are, and what you described is exactly what millions and millions of people across any country face. You look at something and you're not sure where to watch it and you're not sure what the plot line will be and often it... I've watched different shows and they start off one way, being macho with a hero, like all 24 type. And then by the end, it's something completely different. They slipped it in. They've really gotten quite clever about it in advancing this agenda of a particular way of looking at the world. And of course, the UK has been one of the world leaders in advancing nudge technology, which is what this really is, that we're encountering in broadcast media, is applied nudge. And, you know, this is another case of a slippery slope. Who's against reducing public tobacco consumption? Oh, you know, we're all for that. We don't want passive smoke. And well then, so then it's okay to use nudge to reduce that. And then, well, who's not in favour of more tolerance against ethnic minorities? Well, of course we all want that. Well, how are we gonna do it? Well, we'll take this same nudge technology and apply it like a great big hammer to that one. And so then we, I'm gonna, this is a sacred cow. So then we have the infamous Dr. Who has suddenly become a femme, et cetera. And that kind of went over like a lead balloon, as I recall. But it doesn't matter. In pursuit of the social engineering agenda, profitability and audience uptake and acceptance are totally secondary to the broader mission of advancing social equity as defined by whomever they are that established all these agendas and try to propagate it globally. Another one that I ran into recently was a CNN broadcast looking at travel and food in Italy. Who isn't interested in Italian food and travel? I mean, that's a great topic. And this is coming off of Anthony Bourdain's suicide and how successful his series was, because I think CNN is trying to pick up the ball. And so they get another actor, a well-known Italian, I'm not gonna beat him up, that tours Italy. And we're in about three episodes and suddenly we're getting propagandized about, in favour of basically Italian socialism, and the importance of accepting immigrants from North Africa and integrating them into Italian society and that these far right people, or go Giorgio Moloney, et cetera. Because anybody who is against immigration is obviously far right by definition. And Mussolini is just right there. Don't like immigration, you're one step away from full-on Mussolini fascism. And so that's how that particular series has gone. As another example is that it's become a platform for advancing these same social theories, ostensibly under the guise of a travel show focused on Italian cuisine. I mean, it pervades everything. It does, and I know that you give 15 examples. You go through, of course, The one that stands out, which you've also known for the last three years is called COVID-19 virtue signalling. Virtue signalling is a term that links with woke. But that maybe took it to a new level of advisories, COVID advisories under videos, giving you the different side. And Andrew Bridgen speaking in the House of Commons, whenever he was talking about vaccine injuries, at the bottom, it kept bringing up all these piece of information of why, what he was saying was rubbish. But even on the COVID stuff, the mask, the double, the triple masking, or people getting a sticker to say they've had a jab, it was in your face, in public, I guess, in a way that we hadn't seen before. And the social distancing, I mean, all these things had no, even though they were cloaked in scientism. Which is, you know, we've got to be careful about using that term, Jill constantly reminds me, because it is actually formally defined as, the belief system around everything that is true and real can be directly perceived and detected. So that's the essence of scientism. And in definition, it's in opposition to, let's say mysticism at one parameter and belief in theology and religion in another parameter, or we could talk about the ancient Celtic religions of paganism, for instance, all of that being in opposition to this belief that only things that we can measure and detect are true. But the term scientism has been kind of transformed a little bit with Dr. Fauci being the poster child of scientific truth is that which is asserted by largely the government and government agents that their interpretation of scientific truth is the one and only truth and there shall be no debate. And the BBC and the Trusted News Initiative have become the guarantors and the enforcers, of that logic that the only scientific truth, quote-unquote, in this post-truth, post-modern world is that which is advanced in the approved narrative endorsed by the government. And you start off, right at the beginning, it is alphabetical, but the 15th, you talk about anti-religious bias, and I'm shocked at how we see that so prevalent, that those with a belief system, and often a belief system in Christianity, are hateful or certainly hypocritical or, I mean, the level of vitriol that is attached to someone who believes something and I guess the whole issue of belief of absolutes is completely alien to a woke agenda where anything goes and truth is subjective and that level of mocking and ridiculing is dangerous certainly in a country as in yours based on biblical foundations and Christian truths and yet now we are encouraged to attack the very foundations on which our societies were based on. Right, and this is all rife with paradox. So you point out that Christianity, and in particular, traditional Christianity, and in most particular, this new insurgent movement within the Catholic Church in opposition to the Pope called Trad-Cath, those folks are in particular demonized for the sin of wanting to receive the liturgy in Latin. You can't make this up. It's so intrinsically absurd. And the bizarre paradox is that, in parallel, the prior religious orientation that was so heavily weaponized, that being radical Islam and Islam, you know, generalized Islam suddenly is aligned, in a paradoxical way with the woke slash progressive or approved agenda, in this enormous rift that has occurred in the context of, let's say gently, the IDF Gaza conflict. And I absolutely don't want to go there because I find that information landscape to be so heavily contaminated with very advanced Psy-War capabilities on both sides that once again this is absolutely a post-truth, post-modern battleground of two highly developed Psy-War capabilities going head-to-head on the world stage in which we could debate who are the victims and who are the perpetrators here, but I think truth is absolutely on the victim list. It's become completely subjective, and that's what's happened throughout all of this, is, as I keep saying, this is postmodernism, in which truth is a subjective quality that is, not intrinsic, but rather is subjective and is the product of exertion of force and capabilities in the information landscape, and is defined by those who are able to dominate that information landscape. Truth is entirely subjective and is a function of whoever is the dominant party in what I call this post-modern golden rule, those with the gold make the rules. And it's now those with the gold define truth. It's so true. We've just had today, there was Rishi Sunak, sadly, our Prime Minister here in the UK, little in substance, little in size, little in vision, but Rishi Sunak- Well, now don't be staturist. But Rishi Sunak, he was talking about a new AI Institute, which the UK is setting up, and it's going to be the world's first AI Safety Institute. And he talked about, we want to have a AI consensus on what's good and bad, just like we have a climate change census. Right, so AI has been sold to the public as a neutral arbiter of truth, but we know from the various AI applications and algorithms that are available now. That AI and machine learning and deep learning are very much a consequence, a derivative, of the source information that is used to build those algorithms, that train them. And so by selecting the information, so for instance, it's just like Wikipedia. Wikipedia defines truth as basically the work product of corporate media, which is to say the Trusted News Initiative. So if it's endorsed by TNI, then it must be true. And that is what comes out in those. So it's not even, it's beyond scientific papers. It's whatever large corporate media puts out with their spin becomes the only allowed input vector for Wikipedia. And this is done, you know, when we now know that Wikipedia is being edited in real time algorithmically by our intelligence community, including your lovely MI6. And so it, you know, people still perceive wiki as a source of basically akin to the Encyclopaedia Britannica, but it's absolutely not. Now, I haven't dove into how badly Encyclopaedia Britannica is being manipulated. I don't know the answer to that now. But the AI-based engines have absolutely been shown to have political biases that are, you know, apparently derived from their training source material. And so, for instance, the other day, Steve Kirsch gave an inquiry to one of them, that I was not familiar with previously and asked if anybody, that had been a anti-vaxxer had turned to being in favour of the vaccines. And he was very comforted by the fact that he was not so identified. But myself and Jessica Rose, and there was another person, were identified as having previously been anti-vaxxers. I'm the vaccine developer anti-vaxxer who invented RNA vaccine technology. That, you know, you have to, this is for, you know, park your cognitive dissonance is irrelevant. But then had now strongly endorsed the COVID vaccines, and indicated that everybody should get them. Absolute lie, absolute propaganda, none of that true that was listed, but that's what spat out in response to that question. And with Jessica Rose, it asserted that she was a former beauty queen. I've never heard her talk about that before. I got to ask her about whether or not she was once a beauty queen. And also went into the same kind of a flip-flop, which I know has not happened. So it's, those of us, and this is another part of my background that most people are unaware. For the first two years of my undergraduate, I was a computer science student and actually took top honours for that time. I just didn't wanna spend the rest of my life in a basement looking at a CRT tube. I was taught early on, garbage in, garbage out. With any program-based algorithm that abstracts from data, your source data will determine the product. You can have the most sophisticated deep learning algorithm, but if it is employing a biased data set, it will give you a biased answer. And that's absolutely what's happening, but it's being pitched to the public as if this is a godlike, neutral arbiter of truth, and it is so easily manipulated. The question is, is Rishi in the cohort of... I don't really understand the technology, but it seems awfully cool. And this tension always exists between nefarious intent and incompetence. And is he merely an incompetent or is he aware of how readily artificial intelligence algorithms can be manipulated? I don't know the answer to that. The dangerous thing is, I think he is aware, because I think his wife's family made the money off tech industry, partially through tech, so he should be aware of it. Exactly. He should be a sophisticated user, and then the derivative of that would be, this is not a guy that can be trusted because he's misrepresenting truth to the British public. I know that you would probably be shocked and we will probably be de-platformed and severely, maybe I better be careful because I want to fly back into London to help out Andrew on December 4th, so I don't want to get arrested when I land at Heathrow. Well, we'll see how that happens. You may just stay in the UK for a while and that will keep you. Government hospitality. You started by talking about that Netflix series, the slipping in of man love, as you said, and I've been watching another BBC programme and three series in, oh, suddenly now there's the other side, woman love and that slipped in, doesn't fit with the story, doesn't add to it at all. But in your article, you talk about bad masculinity and where male behaviour is inherently toxic and negative. But then again, the fun side, because you have to sit back and with a smile and begin to mock the chaos and confusion and people trying to jump through hoops and put these together. You've got the whole infinite genders and how that works. And I was sitting watching GB News yesterday and they had someone on who I think was, I'm not sure whether male or female, a strange hybrid. They were talking about- What's wrong with you? It's irrelevant. I no longer know what it means a trans woman. A trans woman, I think, okay, just go back to basics. How did the person start? That's where I need to start with. But this confusion, if male psychic or identification is toxic, then what happens if a woman then decides she's a man, is that then still toxic or is that now acceptable? And this chaos and confusion. Yes. Oh, like I said, the cognitive dissonance associated with this, which has no well-structured underlying logic, is profound. And so you have, like with any cognitive dissonance, you have to just park that, don't confront it, because it will drive you mad. It's, cognitive dissonance is one of the major sources of psychological pain, and, but only if you confront it. And so for your mental health, you should, Peter, you should really need to stop thinking about things like that. And, go back and and reread 1984, but consider it to be a guidance document rather than a warning document, and it will all be fine. Along those lines, I strongly, in terms of content to consider in streaming, often overlooked is this lovely little sci-fi piece with Uma Thurman, as I recall, called Gattaca. And I strongly recommend, GATTAGA is actually intended to be a DNA sequence in this particular sci-fi piece, which is very well-produced, way ahead of its time, and absolutely predicts the almost as prescient as 1984 was, absolutely predicts this new reality, postmodern reality, that we're walking into, where your genetics define, who you are and what you're allowed to be on behalf of government industry and everything else. So that absolutely should be on everybody's watch list if you haven't already seen Gattaca. But this logic that you're talking about, toxic masculinity of course, is what Jordan Peterson, has been so reviled for speaking against. And of course we all know that Jordan Peterson is now, subject to re-education by edict of his local health authority, I think in Toronto, as I recall. And they are also subjecting other physicians to what re-education processes that have had, you know, sinned by prescribing ivermectin or hydroxychloroquine for early treatment and written statements recommending that individuals should not, they should not necessarily be required to take the mRNA vaccine products or other genetic vaccine products, which the government so heavily advocates. And in those re-education processes, which the individuals are required to pay for, by the way, the state doesn't pay for that re-education. it's like three to 5,000 bucks. And at the end of which you have to write a letter demonstrating that you have successfully incorporated the teaching of the re-education process and that you are sufficiently contrite for your past sins. And if you do not show sufficient contrition in this letter, I mean, this is full on Soviet, right? If you don't show sufficient contrition, Then you will have to pay for another round of re-education ad nauseum until the overseers believe, they become convinced, that you have provided the necessary written, signed documentation that demonstrates that you are requesting humbly forgiveness for your sins, and that you have indicated clearly in this public document that you will not sin again. And so anybody that asserts that this is not actually a false religion isn't paying attention, because that's what we have, this, whatever you want to call it, wokeism or whatever, this new censorship, industrial complex, propaganda infrastructure that so much of it traces back to UK social science, I'm sorry to say, is now enforcing itself on the population using the classic totalitarian methodology. And I'm reminded, someone pointed out to me the other day when I was at Shannon Joy's event in Rochester, that Mattias Desmet, when he speaks of totalitarianism, he's not, a key differentiator between authoritarianism and totalitarianism is that authoritarianism under a dictatorship or other structure is a minority imposing its will on the population. Totalitarianism, it is the totality of society that is acting in this way. It is a social consensus, in this case a manufactured one, but it is a social consensus that this is the way we shall be. And one of the key factors in when totalitarianism emerges in a society and basically integrates itself into the entire system is that you can turn over the leadership, ergo Rishi and the prior prime minister that you guys, that brief stint that you all experienced. Forty-five days. You can replace the leadership, and it will not change the process until the entire population wakes up from what's happening. Because there will be new leaders emerge organically from the society to fill that role of dear leader. Because this is absolutely a function of a deep psychological process that is consumed the society. So we can all in our little bubble of resistance cell, Is it 20% of people? Is it 5%? We can go back and forth. We can all laugh at the sheep and what's going on and point out their logical inconsistencies. It's irrelevant until such point as people literally wake up and recognize that this is, at a minimum, not meeting their needs. You know, this is the classic question, are you better off than you were earlier on under this, I guess it's conservative government that you have that is not acting very conservative? Yeah. are you better off now than you were then? And at some point the culture says, no, we need a change. And they collectively wake up, but when that's gonna be, is it gonna be next year? Is it gonna be next decade? Or is it gonna be 100 years from now? There's no way to predict. Yeah, I'm just imagining, sorry, I'm just imagining Jordan Peterson writing a letter of apology to the Canadian government saying how wrong he was. I think this actually could be a series, you could have different, I'm sure you would love to do one program, you know, many people, a whole 45-minute fly on the wall, high-profile individuals writing I'm so sorry letters, sounds perfect. Yeah, I think Babylon Bee needs to take this up immediately. Hey, there's one more. Russell Brand ought to do this. This is absolutely made for Russell Brand. I think you need to patent this idea immediately. Russell Brand's an intriguing character. I'm not going to go there. One other thing I'd pull out, distorted racism, and this pitting people against others. Living in London, being in a very mixed culture, coming from Northern Ireland, being in a mono culture, and enjoying that mix and all that brings, and then this push to, no, you must see racism behind everything. That on top of everything else, it is, the tension that is developing in society is frightening. The people are encouraged to pit themselves against someone else instead of agreeing to disagree, not allowed to agree to disagree. And if you don't agree, you're wrong, you must be hated, you must be mocked, you must be attacked. You see as well in the States that that tension is bubbling under the surface. And it's just, I mean, we're laughing at our Canadian colleagues, but we know that they have been at the tip of the spear in advancing a lot of these agendas, most notably the de-banking, is the most egregious example. And Nigel can, there in the UK, can speak eloquently at length about the debanking agenda and its intended linkage with social credit system. Yeah, so the question is, I think for all of us, again, this grappling with the tension between incompetence and nefarious intent, which is so hard to disambiguate unless we're mind readers or we are able to get through Freedom of Information Act, the smoking gun documents that establish nefarious intent. But this division of society, again and again and again, repeated division into subgroups on top of subgroups on top of subgroups, absolutely has the appearance of serving the interests of large financial and power interests, which may be transnational, multinational, or globalist. We have these euphemisms that are all used. Just as the hypothetical, not saying necessarily this is what's happening, but if you had, let's say, traditional monarchists or large banking conglomerates. Or we can go down the list of potential actors that were interested in further enhancing their power and financial base at the expense of what ostensibly is a self-governing populace, then I hesitate to use the term democracy. And I would get attacked if I used the term democracy in the United States, because we don't technically have a democracy, just like technically you don't have a constitution. But it certainly has appeared to be in the interests of these very large concentrated power blocks that are led by a very small number of individuals who happen to be, have accumulated, you know, calling it wealth is kind of almost a misnomer, assets in power that they have, they and their families or progenitors or associates have historically exploited to further concentrate and exert their influence over world affairs. That's my attempt to be politically correct and wrap things with words that the average person would just say, well, they're all corrupt and they want to run the world. And as I get, again, I cite back the postmodern golden rule, those of us of a certain age recall when the golden rule was due unto others as you would have them do unto you. And in the postmodern world, the golden rule is those with the gold make the rules. Maybe it's always been that way. I don't know. Maybe I was just naïve back then when I was listening to my pastor speak on the lectern in the Episcopalian church that my parents used to go to. And that's another thing that I find fascinating When you think about it, we all, many people used to... I'll say, make fun of the Church of England in its leadership in advancing what we now recognize as a woke agenda. But it is increasingly normalized across much of the Christian theologic space, notably including the Catholic Church, the current pope. So maybe we got it all wrong. Maybe the Episcopalians were actually the tip of the spear in advancing the new world order. I don't know. But it kind of increasingly looks that way. One would never imagine the Episcopalian clergy as, I don't think any stereotype would label them as the avant garde cutting edge of social change. And yet, so it would seem. Yeah, that fight for truth in the church, whenever it's there in black and white in the Bible. Can we finish on, word of the week, adulteration, this, I guess, Great memes circulating, with, I guess the Sermon on the Mount with the stone tablets, with the 10 commandments and the statement, thou shalt not commit adulteration. And no, it's not adultery. Yeah, so this is a scientific technical term and a regulatory term. It's actually entrenched in American federal law and throughout the world in regulatory statutes, and policies that have largely been enforced and structured, through the International Committee on Harmonization, throwing out some buzzwords there for all of you wokes, which is kind of the international body that has harmonized it. You know, that's another good woke term, very important in European politics. We all want to be harmonized and aligned. But harmonized regulatory policies globally, so that Mr. Pharma doesn't have the inconvenience of having to restructure their regulatory documents for each nation state that they submitted to. So harmonization is important, and a key term throughout all of this is adulteration, which is in its simplest form. It is the incorporation of adulterants or impure material into, in the original American statute, food, devices, or drugs. Remember, we have the Food and Drug Administration that's responsible for this. And it goes back to the time of the scandals that Upton Sinclair revealed in his classic works, literary works, concerning the Chicago slaughterhouse environment and contamination of food with rat faeces and other materials. So rat faeces or other awful, you know, excrement from animal species contaminating foodstuffs is absolutely considered to be an adulterant. But technically in the drug space, adulteration consists of inclusion of materials in a final drug product, that are not specifically disclosed in the label. Remember the label also includes that little package insert that goes in the box that says all those things that nobody ever reads in the very fine print. But basically, as one is allowed to market a drug product, typically that requires the final drug product, I'll say this way, is defined as the sum total of everything that goes into making it. So the manufacturing process, the testing and release, the regulatory documentation, the documentation about the clinical trials, all of that, it's not just the stuff that's in the pill or in the syringe, but it's all that stuff, that documentation and information that surrounds it, that cloud of information. And so technically, if a regulatory authority together with a manufacturer disclose that, well, In fact, in our product, there is a certain fraction of rat feces, but we have a test for rat feces, and those rat feces cannot exceed 0.001% or whatever the thing is. That type of thing makes that not an adulterant. If it is disclosed and you have some parameters around it, then that's acceptable. What's happened here with these RNA genetic products is that two things have not been disclosed. And basically, they have been detected, by two different laboratory groups, one in the United States and one in the University of Guelph. You'll remember University of Guelph being the home of Byron Bridle. And so now we have another Guelph dissenter, solid, rock-solid scientist in his group that does DNA sequencing that these two groups have acquired at great difficulty because, of course, this is forbidden in most nation states to be able to analyze the vials that Pfizer or Moderna give us. We're told to just accept them that they are as they are asserted to be, which has never been the case with other drugs. Always a national authority like Paul Ehrlich or the European Union will independently verify that what is in the vial by random sampling is in fact what pharma says is in the vial. And it's within those limitation parameters that are predefined in the regulatory package. But it turns out that they were asleep at the switch or wilfully ignorant or we can extend that out, and hopefully someday we'll have the documentation to allow us to discern how the heck this happened. But we are hearing now more and more a cascade of mea culpa statements, from European Medicines Agency, Health Canada, Australian regulatory authorities, yet to hear this from the FDA for some reason, that in fact, we're guilty. We weren't aware. Mr. Pharma misled us. Pfizer, Moderna, whomever, misled us. And in fact, there is quite a bit of these small linear DNA fragments contaminating the final drug product. And by the way, the DNA source, a circular piece of DNA that was used to manufacture the RNA, it was grown in bacteria. And that leads to another whole can of worms is the endotoxin contamination. And whether it's been adequately monitored. Endotoxin being something that's known to cause shock in humans when injected, humans turn out to be exquisitely sensitive to injected endotoxin compared to many other species. And that may have something to do with some of the anaphylactic reactions that patients develop within the first 24 to 48 hours. Certainly that is consistent with endotoxin contamination. but also that we have this DNA contamination. And so, because it wasn't disclosed. And furthermore, the composition sequence of the plasmid from which these short DNA fragments are derived was not disclosed. And in particular, the existence of some sequences derived from simian virus 40 were not disclosed. And a lot of then this constitutes an indulgent. And the EMA and some of these other health agencies are now basically saying, not our fault, Pfizer didn't tell us about it. And Pfizer is coming out with statements saying, well, we didn't tell you about it because we didn't think it was important. I'm paraphrasing, but that's the essence of the argument. And it turns out this is not okay, because short linear DNA fragments that are produced by degrading these plasmids are among the most highly active DNA molecules if you want to mutate a genome, let's say, of a cell line or an experimental animal. And normally, that level of DNA contamination would be below the threshold that has historically been imposed for vaccines. So it's a chronic problem, DNA contamination in vaccines. Basically, it was a major reason why the Solvay program that I was on got killed, because they couldn't get rid of all the DNA they needed to from the cell lines that were growing the flu. That's a tangent. But it's a known problem in biologics manufacturing. And so technically, the threshold level of DNA fragments that are there are below the historic allowed DNA contamination. But it's kind of apples and oranges because with an injectable, let's say, a flu vaccine, it's not designed to be a polynucleotide delivery system. Okay, but what we have here with these RNA products, thanks to the enabling technology created at the University of British Columbia by Peter Cullis and his colleagues after decades of work, by the way, they were the ones, if anybody's going to get the Nobel Prize for the enabling technology advance, it was those guys, not the pseudouridine people, but that's another tangent. So shout out to Peter Cullis, I guess, for enabling this technology in vivo. But that tech is agnostic about whether it's DNA or RNA. And so you have the most potent non-viral delivery system ever devised by man being formulated with not only RNA, in RNA that isn't really natural RNA, it has a very, very long half-life and it's immunosuppressive if that was by design, but also with these short DNA fragments, which are known to be highly mutagenic. It hasn't been proven in these formulations, but the literature is explicit on that and abundant, that short linear DNA fragments will drive mutations in your cells in your body, if it's injected through your body. And then perhaps most worrisome, we know that these formulations, based on the Pfizer common technical document that was submitted, that's the non-clinical package to get authorization to proceed. That was kind of the last shot at doing the real toxicology, revealed that these particles have a surprisingly high affinity for ovarian tissue. Which is where germ cells reside in the form of eggs, ova, and not tested was whether or not these ova take up these particles, but certainly the ovarian tissue in general does. And then of course, we also have the recommendation that pregnant women should be receiving these products. And we know from the literature that these products cross the placenta. So they are getting into baby, and baby is in first trimester in a highly, rapidly developing embryonic environment. And so my determination is this is a true risk. It is one that will manifest in the form of somatic cell cancers, particularly lymphomas and leukaemia's, which by the way, we do happen to be seeing a surgeon inexplicably. But if you wanted to, in an experimental model, drive the development of leukaemia's and lymphomas, you could use retroviral gene therapy vectors. That's why it basically killed retroviral gene therapy was because of these side effects of leukaemia's and lymphomas because of insertional immunogenesis. And you could do it experimentally if you wanted to discover oncogenes or tumour suppressor genes, because that's how this technology was used. You could deliver, using these cool new formulations from Cullis et al, short DNA fragments, and they would absolutely disrupt the genome. And so there's the cancer risk, and there's obviously a germline foetal development mutagenesis risk. And I'm confident enough, because this was the area of molecular biology that I literally cut my teeth on originally. Mouse memory tumour virus, intentional mutagenesis, and searchable mutagenesis in order to search for oncogenes and tumour suppressor genes, under the mentorship of MD, PhD pathologist, one of the first molecular pathologists who had just finished a sabbatical with Bishop Envarmus, who got the Nobel prize for discovering oncogenes. Okay, so I know this area, the literature is clear and explicit, and I'm so confident about these observations that I'm willing to put my reputation on the line and say, this is happening at some frequency. We just don't know what the frequency is right now. Well, it's a huge topic and it's a perfect end. And I know another reason to get your Substack is you've just put an article up on this, republished a trial site news piece on this and people can delve into that. But Robert, I always appreciate your time for coming on and congratulations on having a speaker in the house. Now the Republicans can continue to use their majority for to do nothing. So well done. Oh what a bizarre world we live in. So yes, all true. I'm from Louisiana and we now have a Republican governor in Louisiana who I've known and worked with. It's been my pleasure. And the new speaker talks the talk, so let's see if he'll be able to walk the walk about all these various issues. And time will tell on that, but they are under a pretty short deadline to fund the government. And are we going to continue to see basically kick the can down the road, continuing resolution, or are we going to see the Republican marginal Republican majority in the House that ostensibly is the ones that are supposed to create the budget actually do what they have been saying they're going to do and take up all these independent budgets for funding the various public agencies, including Department of Defense Homeland Security with CISA, the intelligence community and their black budget, and all of these other weird and wonderful agencies that we have developed here in the United States that are bleeding us all dry and driving... The other day, I was reading Doug Casey's International Man, and he used the metaphor that the American government is like Wile E. Coyote in the Roadrunner series. It's already run off the edge of the cliff and it just isn't aware that it's about to plummet down to the bottom and get crushed. We are way past the danger zone in terms of our indebtedness. I don't know what's gonna happen. 100%. For the viewers and for listeners, make sure and follow Robert if you don't already on his Twitter or on GETTR, on the Substack, and lies my government told me, is a perfect Christmas present gift. So do look out for that. Do get a... Oh yeah, please. A copy. And pass out, but- I've heard it referred to as an important historic document. Oh, it is. Oh, it is. Because it was written in real time. And that could never be written today because many of the references have been getting scrubbed from the internet. Yeah. Yeah, we've seen that. Absolutely. Robert, thanks so much for coming on, sharing your latest Substack and a number of other thoughts. So thank you. Thanks, Peter. Anytime.
Let's get blue on today's solosode of The Red Light Report! The last couple of weeks you have likely heard and/or learned about BioLight's newest innovative product, BioBlue, which is an enhanced methylene blue and mitochondrial supplement. Some of you are even using it right now! But I felt it would behoove all of us to take a deeper dive into this mysterious synthetic textile dye that happens to seemingly be one of the most profound health, wellness and mitochondrial-boosting substances available. And it is a major synergist when combined with red light therapy! So today, I thought it would be great to review the top 10 uses for methylene blue, as professed by scientific research. And while I don't want to give away too much in this intro, I feel obliged to tell you a few of the things that I find most exciting and promising about methylene blue:1.) Secondary to its ease of crossing the blood-brain barrier, a vast majority of the methylene blue consumed concentrates in the brain, which is why it has virtually endless brain health benefits (i.e., reduced symptoms of Alzheimer's, Parkinson's, dementia, depression, PTSD and improved cognition, mood and energy);2.) Methylene blue has an innate intelligence to help whichever cells are most sick, diseased and/or cancerous first before moving along and helping other healthier cells;3.) There are only two known interventions capable dissociating nitric oxide from the cytochrome c oxidase (which then allows oxygen into the mitochondria for more efficient energy production; remember, the more energy in a cell = less disease state/more vitality in cell): red light therapy and methylene blue. Remember, none of this information is a substitute for medical advice. But just like when I learned about red light therapy for the first time and was in awe at its endless health benefits, I am equally impressed by the limitless potential of methylene blue. Both are safe, efficacious and likely have a pivotal role to play for anyone looking to optimize their health and longevity. As always, (red) light up your (blue) health! - BioBlue, BioLight's newest product: (0:00) Diving into methylene blue: (7:30) Malaria cured in 48 hours: (10:48) Brain function and methylene blue: (12:29) Metabolism and methylene blue: (21:07) Top ten benefits of methylene blue: (21:56) Combining methylene blue and red light therapy: (29:16) COVID-19 and methylene blue: (30:03) Alzheimer's and Parkinson's and methylene blue: (33:30) Dosages: (38:48) Hallmarks of Alzheimer's and methylene blue: (39:54) Metabolic hallmarks: (41:04) Red light therapy and methylene blue for dimensia: (43:11) Cognitive enhancement: (45:23) Depression: (48:56) Autism: (54:41) Endotoxin in the gut: (1:01:02) Pain relief: (1:03:49) The heart: (1:09:53) Cancer: (1:11:48) Red light therapy and methylene blue: (1:14:07) Methylene blue therapy for cancer: (1:16:14) - Book referenced from the episode: The Ultimate Guide to Methylene Blue, by Mark Sloan - Exclusive 20% discount on BioBlue for The Red Light Report listeners! Use discount code: bioblue20 - Kindle version of Red Light Therapy Treatment Protocols eBook, 4th Edition - To learn more about red light therapy and shop for the highest-quality red light therapy products, visit https://www.biolight.shop - Dr. Mike's #1 recommendations: Grounding products: Earthing.com EMF-mitigating products: Somavedic Blue light blocking glasses: Ra Optics - Stay up-to-date on social media: Dr. Mike Belkowski: Instagram LinkedIn BioLight: Instagram YouTube Facebook
Follow us on LinkedIn, X, Facebook and Instagram. Visit us at https://www.bio.org/The horseshoe crab has endured for over 450 million years. It has survived several mass extinctions including the one that killed off the dinosaurs. One reason for their incredible resiliency is their ability to fend off bacterial infection. Their blood contains cells that clot around invading bacteria, thereby protecting them from the attacking toxins.In this episode we talk with three experts about how this animal's unique blue blood has become essential to modern medicine. We also talk about why horseshoe crab populations are dwindling, and what biotech is doing to address the shortfall.
-Overview of Chronic Inflammatory Response Syndrome -Testing buildings before "ERMIs" were available (tape-lifts, VCS testing) -Utilizing "ERMIs" in the field -The develop/value of HERTSMI-2 sampling -Patient recovery and passing HERTSMI-2 scores -Actinomycetes (gram-positive bacteria) research -Endotoxin (gram-negative bacteria) research -Putting it all together (What Dr. Shoemaker recommends for testing)
A conversation with Georgi Dinkov about the connections between elevated estrogen, histamine, serotonin, and endotoxin - four components to what often becomes a viscous cycle of estrogen dominance, gut issues, mood issues, histamine issues, and more. Learn why serotonin is not simply "the happy hormone" and excess serotonin produced in the gut is actually a bad sign. Estrogen is not simply the female hormone, and elevated levels of estrogen are often missed due to low or normal blood levels, but high levels stored in the tissues. Histamine intolerance should be addressed with the greater picture in mind. improving the metabolic rate and addressing estrogen dominance. Elevated endotoxin is toxic to the entire body and often exists in states with higher levels of estrogen, serotonin, and histamine. Georgi provides some details regarding the corruption within big pharma, SSRIs, and other drugs. We also discuss the benefits of aspirin and progesterone, progesterone and thyroid during pregnancy, chicken health, and a few other random topics. Towards the end, you'll learn some practical steps you can take to break the estrogen-histamine-serotonin-endotoxin cycle to start alleviating many symptoms and further along your healing journey. CONNECT WITH GEORGI IdeaLabs http://idealabsdc.com/ Twitter @haidut Blog/articles http://haidut.me/ You can connect with us on Instagram @strong.sistas Or our farm Instagram @angel__acres Learn more about our course, Rooted in Resilience & our exercise membership, Strong Training on our website www.armstrongsisters.com Thanks for listening :-)
In this episode of The Thermo Diet Podcast Tyler interviews Georgi about everything related to serotonin, histamine, and endotoxin and how this relates to our gut.
Do you know how endotoxin tests are carried out? Well, in this interview with David Guy from Cape Code Associates, you'll find out ! David tells us about recombinant reagents, a new technology that aims to replace LAL (Limulus amebocyte lysate) and stop using horseshoe crabs, these prehistoric animals whose blood contains the precious enzymes necessary to perform these famous tests!
Die Themen in den Wissensnachrichten: +++ Jugendstudie: Vielen geht es psychisch nicht gut +++ Allergene auf Smartphone-Oberflächen +++ Schaltsekunde soll abgeschafft werden +++ **********Weiterführende Quellen zu dieser Folge:Trendstudie Jugend/ Simon SchnetzerConférence générale des poids et mesures/ RésolutionsAnnals of Allergies, Asthma & Immunology/ INNOVATIVE COMPOUNDS TO REDUCE SS-D-GLUCANS, ENDOTOXIN, AND ALLERGENS NEWLY DISCOVERED ON SMARTPHONESGlobal Change Biology/ A regime shift in the Southeast Greenland marine ecosystemJAMA Health Forum/ Association of Recreational Cannabis Legalization With Alcohol Use Among Adults in the US, 2010 to 201**********Ihr könnt uns auch auf diesen Kanälen folgen: Tiktok und Instagram.**********Weitere Wissensnachrichten zum Nachlesen: https://www.deutschlandfunknova.de/nachrichten
Constantine joins us once again, this time to talk about the beast that is ENDOTOXIN and how we all feed it and how we can manage this issue.Endotoxin is not the ONLY enemy plaguing our guts, but it's certainly one of the biggest.You can reach Constantine here constantine.kanargelidis@rogers.comAlso mentioned in the podcasthttps://us.onasnatural.com/ USE COUPON CODE DEAN for 10% off all productsAlso mentioned in the podcastJust Thrive Spore Based Probiotics and Prebiotics to help manage endotoxin use your coupon code HGG20 and click HEREAlso mentioned in the podcastsign up for your own PRL account for FREE and then you'll have access to the highest quality supplements like MEDI-CLAY FX to bind to toxins and help eliminate and HCL and DIGESTus this coupon for 30% OFF 30%_4G7W57_RMsign up HERE Support this show http://supporter.acast.com/stuff-your-doctor-should-know. Hosted on Acast. See acast.com/privacy for more information.
If you don't already, follow Jayden Miller @theresearchcowboy - I love his handle so much! But Jayden is such a wealth of knowledge. He's only 23 years old but has his own health story involving hypothyroidism and low testosterone, which he's managed and overcome with a pro-metabolic approach.Through his own journey, Jayden came across Ray Peat's work, and the rest is history! In this episode, we chat about:➡️Jayden's background ➡️What is serotonin➡️Why it's not the “happy hormone”➡️The role serotonin plays in energy production and metabolism➡️How serotonin impacts other hormones➡️How your gut affects serotonin levels➡️What processes help regulate serotonin➡️Signs of high serotonin➡️The role serotonin plays in depression➡️How antidepressants work➡️What is more beneficial for depression➡️Things you can do to improve your gut health➡️Foods to consume for better gut health➡️What to do for bad endotoxin issuesLINKSFollow Kitty on Instagram
00:00 - Ray's point of view of the competition between empires and how it relates to coronavirus 02:21 - The CIA's role in Ukraine 07:43 - Ray's view of nazism 08:36 - Isn't Ray anti-war? 11:53 - Where does Israel fit into the empire game? 14:13 - What will the elite do when the U.S. becomes a third world? 15:27 - How is Ray inoculating himself from food shortages? 17:04 - What does Ray think will happen to big cities? 17:41 - Ray's current view of the long-range harm of the coronavirus vaccine campaign 21:45 - Were the vaccines more effective or less effective than the elites anticipated for creating harm? 24:36 - Is Ray having a harder time communicating with the public? Is meaningful work the guiding principle for Ray's life? 27:52 - Who could endure more stress: an EFA deficient rat or a germ-free rat? 30:10 - Fentanyl, "addiction," and euphoria 32:12 - Why has no pharmaceutical company produced pregnenolone? 39:10 - "...Anyway, the important point for understanding some of the present cultural differences between Russia and the West is that Russia has for centuries felt the influence of Aristotle, and has not suffered from the influence of Plato or Calvin..." — Mind and Tissue by Ray Peat (1973) 42:00 - How to purchase Ray's newsletter, books, Progest-E 43:24 - What is Ray working on?
Hosted on Acast. See acast.com/privacy for more information.
Endotoxin testing serves a vital function in pharma quality control by ensuring that products are safe for patients. But the traditional approach to Bacterial Endotoxins Testing (BET) is an area ripe for disruption and improvements. Now, the Sievers Eclipse BET platform by Suez offers new levels of automation, compliance and sustainability, ushering in the next generation of BET. Learn more about Suez: https://www.suezwatertechnologies.com/ Read the transcript: https://www.pharmamanufacturing.com/podcasts/off-script-a-pharma-manufacturing-podcast/solutions-spotlight-a-groundbreaking-approach-to-bet-testing
00:00 - Skip setup music, catch-up, life in 2021, inflation, do you need a vaccine to live in Mexico? 23:09 - Low testosterone (T) and/or high estrogen/T ratio predict COVID-19 mortality 27:53 - Vitamin D3 anabolic, calcitriol catabolic for muscle/thymus 33:19 - Idealabs update: 10-Methoxy-Harmalan, vitamin K2 in olive oil 58:33 - Endotoxin (LPS) may directly cause cancerization of normal cells 39:53 - Placebo effect plays role in antidepressant impact on anxiety 49:11 - Omega-3 increase risk of atrial fibrillation (afib) 51:53 - PUFA are remarkably estrogenic, toxic to the ovaries 58:08 - Serotonin, SSRI drugs may be the cause of IBD, Parkinson's, diabetes, etc. 01:09:34 - Restoring oxidative metabolism has curative effects on cancer (leukemia) 01:15:18 - Vitamin K3, more idealabs updates, outro
I caught with Brendan Tindall, Global Solutions Manager – ENDONEXT, Industrial Microbiology, bioMérieux last October to learn more about Recombinant Factor C (#rFC) for Bacterial Endotoxin Testing (#BET).You can find out more here https://lnkd.in/eV-Je-AYou can view many more interviews and demos from many other industry experts across Pharma, Bio-Pharma and Life Science on my YouTube channel RSK Life Science Media https://www.youtube.com/channel/UC9vdET2xLlGweqveW6JmQdQ#rFC, #endotoxintesting, #BET
I caught up with Nicola last November to discuss #HorseshoeCrab Populations, #LAL and #Recombinant alternatives, and the impact of #Covid19.You can find out more by visiting www.criver.com and search endotoxinAgree with Nicola, disagree? Feel free to comment below.You can view Nicola's video at last years #Pharmalab herehttps://lnkd.in/ew7SX6qYou can view many more interviews and demos from many other industry experts across Pharma, Bio-Pharma and Life Science on my YouTube channel RSK Life Science Media https://www.youtube.com/channel/UC9vdET2xLlGweqveW6JmQdQ#endotoxintesting, #BET, #Recombinanttesting
On this episode of the podcast, I go over the methods, results and conclusions of a study to test the Wim Hof Method. Who is Wim Hof and what is his method? Wim Hof is a Dutch extreme athlete who has broken several crazy world records such as longest time in direct, full-body contact with ice or farthest swim under ice earning himself the nickname of The Iceman. Wim claims that his feats of extreme endurance are not something unique to him but rather something that can be developed by anyone over time using his method. The Wim Hof method consists of 3 components: meditation, cold exposure and breathing techniques.In the study, Wim taught and trained his three-part method to study participants for less than a week and they had approximately an additional week to practice on their own, after which they were tested by getting injected with endotoxin. What is endotoxin? It is a molecule from bacteria which when injected has been shown to cause the subject to experience fever-like symptoms as the body's innate immune system responds. That is everybody except Wim Hof, he had reduced symptoms and a difference in his immune response. So the question is: Could other people trained by Wim also be able to have a different response to getting injected by endotoxin after learning and training the Wim Hof Method for a short period of time? So if all that sounds interesting then this podcast episode is for you.If you have any questions or comments and need some clarification, please send me a message at newsletter@jmartfit.comConnect with J-Mart on Social MediaInstagram - https://www.instagram.com/jmartfit/Twitter - https://twitter.com/jmartfitFacebook page - https://fb.me/jmartmovesMedium page - https://jmartwrites.medium.com/Newsletter - newsletter@jmartfit.comTime codes0:15 - Intro2:33 - Start of episode3:36 - Wim Hof World Records6:15 - What is the Sympathetic Nervous System?8:18 - What is the Innate Immune Response?10:37 - General Experiment Introduction16:42 - Materials and Methods of Experiment26:44 - What are Cytokines?28:18 - Results of Experiment40:17 - Summary and Discussion51:47 - My Final Thoughts55:50 - OutroYou can also check out my Monday Morning JMART CAST for my unfiltered thoughts and opinions on the platform of your choice:YouTube - https://youtube.com/playlist?list=PLZiptzWGRSXNYGgPEVFFzUGauXObYProZApple - https://podcasts.apple.com/ca/podcast/jmart-cast/id1581359697Spotify - https://open.spotify.com/show/5ZAV5xz6O51MyfovBO6c32?si=-lOyuEe7SNKt8J8oiz-TgQ&dl_branch=1Amazon Music - https://music.amazon.ca/podcasts/53f3b6e8-ca9d-492f-bb68-0282d65344fb/jmart-castReferral LinksWild Meadows Farm — https://wildmeadowsfarm.ca/register?referral_code=V2OMFtc5XYJdBlockFi — https://blockfi.com/?ref=153dfa59Support this podcast at — https://redcircle.com/state-of-health/donationsMusic: https://www.bensound.comSupport this podcast at — https://redcircle.com/state-of-health/donations
I caught up with with Brett , who, as well as being the LAL Production Manager at #AssociateofCapeCode, is also Chair to the Horseshoe Crab Advisory Panel of the Atlantic States Marine Fisheries Commission.The conversation covered....A quick overview on ACC and Brett's background? 00:49Why is it important to test for endotoxins? 01:39Why are horseshoe crabs so important for endotoxin testing? 02:17Are there other ways to test for endotoxins? 03:02What does LAL stand for? 03:56How is the Horseshoe Crab population? Doesn't the biomedical use of the crabs affect it? 04:31What's his response to those people who believe the horseshoe crab populations in the US are not doing well? 05:36What sort of conservation measures are being taken to help horseshoe crabs. 07:27What is ACC's Horseshow Crab Sustainability Project and what recent milestone did it reach? 09:05How has COVID impacted on the demand for LAL 10:28You can find out more about the topics discussed by Brett below.Recent large scale morality study: https://lnkd.in/gqQVgMuYAtlantic States Marine Fisheries commission: https://lnkd.in/ghzk2FFkMassachusetts DMF Compliance reports: https://lnkd.in/gGgx36ztAssociates of Cape Cod Inc. website https://www.acciusa.com/You can view this and many more interviews and demos from many other industry experts across Pharma, Bio-Pharma and Life Science on my YouTube channel RSK Life Science Media https://www.youtube.com/channel/UC9vdET2xLlGweqveW6JmQdQ#Endotoxintesting #LAL #Horeseshoecrabs, #Horseshoecrabconservation #ACCIConservationproject #RSKACCI #BET
00:00 - Skip setup music, catch-up with Georgi, cultural happenings 19:48 - Cellular “memory” of stress can be passed on for 50+ generations 28:37 - Endotoxin (LPS) as a cause of liver disease, obesity and diabetes, HDL 37:15 - Aspirin reduces symptom severity, COVID-19 mortality by half, cancer 46:52 - Idealabs update 54:47 - Metabolically, children are different species compared to adults, pulse rate 01:03:34 - Manufactured citric acid is a powerful inflammatory/allergy agent, gums, MCT oil, carrageenan, silica, titanium dioxide, ascorbic acid 01:10:57 - Doxycycline can CURE melanoma in humans, antibiotics 01:13:37 - Question: what to look for when buying cheese? 01:17:38 - Question: ginger's benefits? 01:18:26 - Question: protecting immunity? 01:20:29 - Question: how are you guys keeping your cool among cultural happenings?
In this episode we discuss: How endotoxin drives inflammation and stress while downregulating our metabolism Why alcoholic fatty liver disease isn't all that different from non-alcoholic fatty liver disease The protective effects of saturated fats in NAFLD How endotoxin and polyunsaturated fats can cause NAFLD Nutrient deficiencies that contribute to energy failure and NAFLD Sign up for the Free Energy Balance Mini-Course here: https://jayfeldmanwellness.com/energy Click here to check out the show notes: https://jayfeldmanwellness.com/ep-67-endotoxin-and-pufa-as-primary-causes-of-fatty-liver-disease-nafld-part-5/ Timestamps: 3:57 – the role of endotoxin in NAFLD 23:43 – how endotoxin drives inflammation and stress while downregulating our metabolism 30:35 – research looking at endotoxin as the cause of NAFLD 38:30 – how endotoxin mediates alcoholic liver disease and the protective effects of saturated fats 44:56 – endotoxin and other damaging factors in the larger context of NAFLD 52:52 – the role of PUFA in NAFLD 1:12:05 – the contrasting effects of saturated fats and PUFA in alcohol-induced liver damage 1:24:22 – how nutrient deficiencies can cause energy failure and drive NAFLD 1:34:57 – how alcohol causes fatty liver and why alcoholic liver disease doesn't vary considerably from NAFLD 1:38:49 – other factors that contribute to energy failure and drive NAFLD, including psychological stress, fasting, low-carb diets, low-calorie diets, excessive exercise protective effects of saturated fats
Celebrating the pod’s 2nd birthday with a new episode and with expressing a deep appreciation for all the listeners who have followed DTS - some from the very beginning!
00:00 - Catch-up with Georgi Dinkov, the unraveling COVID narrative 04:53 - Is being around vaccinated people harmful? 14:43 - Stress (cortisol) causes hair loss, hair loss as a marker of circulatory disease, aldosterone, PTH, estrogen, trilostane, progesterone 20:20 - Estrogens cause obesity / PCOS in women, DHT is protective 27:22 - Low DHT can cause hypogonadism symptoms even in eugonadal men 33:00 - Vitamin D (topical) safe and effective for removing scars 36:45 - Vitamin D deficiency impairs muscles by lowering energy production, nitric oxide theory of aging, CRH, prostaglandins 46:23 - Is Georgi doing anything at the moment to prepare for an uncertain future? 50:10 - Ruby Ridge, Waco, Timothy McVeigh 58:53 - Serotonin and oxytocin promote gullibility, trainability, and servility 01:06:51 - What's happening in India? Bogus PCR cycle threshold 01:10:47 - Endotoxin (LPS) may be the cause of fatal lung damage in COVID-19 01:14:37 - Pyrucet for cancer? 01:16:31 - Silica, present in most supplements/food, activates the endotoxin receptor (TLR4), EMF, Danny purchased a Mercola EMF tent
00:00 - Skip setup music, current events, society, stress, torture 37:39 - Endotoxin behind the mortality of Black Death and 1918 Flu pandemics 53:41 - Sugar (sucrose) lowers stress-induced cortisol 54:38 - Some aromatase inhibitors (AI) are estrogenic 59:38 - Even “non-carcinogenic” estrogens can cause cancer 01:03:38 - Question: cholesterol level, metabolism, androgens, endotoxin 01:13:55 - Question: dental hygiene, antibiotics, intestinal health, methylene blue 01:18:15 - Endotoxin (LPS) as a cause of systemic inflammation seen in obesity 01:26:59 - Question: elevated white blood cell count, leukemia, PUFA, estrogen 01:32:49 - Question: klonopin, panic attacks, progesterone 01:38:12 - Idealabs update 01:42:08 - Outro, live stream schedule
In this episode, Lucas invites Haidut (Georgi Dinkov) onto the show. He goes by the name “Haidut” online and has posted hundreds of highly controversial and thought-provoking studies in the Ray Peat forum. Georgi discusses many of the common issues with modern medicine, ranging from thyroid health, metabolism, sugar intake, Prolactin, Cortisol, Endotoxin, Adrenaline, Progesterone, PUFA’s, Anti-aging hormones, Serotonin, Steroids. If you are into biohacking and optimizing your health, this podcast is for you! Relevant links:Georgi’s Website: http://haidut.me/ Advanced Testosterone Course: https://optimal-health-masterclass-series.teachable.com/?affcode=495781_tvsziayx Georgi’s Products: http://www.idealabsdc.com/
00:00 - Abiogenic petroleum origin 07:03 - Evolution as an energetic process 08:19 - Georgi on "Kali Yuga" 09:47 - Is there an optimal energy source for civilization? 10:18 - Did we evolve from the ocean? 13:58 - Sydney Fox, Vladimir Vernadsky 15:14 - Jeremy England 17:16 - Alexis Carrel, Hayflick limit, Jean-Baptiste Lamarck, August Weismann 21:17 - Are genes the drives of evolution? 22:48 - Do chimpanzees and humans share a common ancestor? 25:06 - Did the human organism develop under harsh circumstances? 28:10 - Does cognitive ability and openness to possibilities relate to the redox balance? 29:11 - "The being of matter is its meaning; the being of ourselves is meaning; the being of society is its meaning. The mechanistic view has created a rather crude and gross meaning which has created a crude and gross and confused society." David Bohm 32:14 - Altruism retards brain aging? 36:09 - Pathology, trauma, self-mutilation, war 41:22 - Child-care, CIA, Gloria Steinem, Jaffe memo 44:20 - "Toxic masculinity," machismo 48:21 - Contraception weaponization, Barbara Seaman 51:26 - Did occupy wall street frighten the power-elite? 54:42 - What distraction was employed when JFK was shot? 01:01:28 - Half the population is suspicious of mainstream media, "vaccination hesitancy" 01:04:42 - 'Doctors say CDC should warn people the side effects from Covid vaccine shots won't be ‘a walk in the park' 01:05:35 - Children can now self-consent to vaccination 01:06:36 - Vaccine trials testing for symptoms of the common cold 01:07:34 - Will the public get spooked when side-effects are discovered? 01:09:44 - How long before side-effects are seen from an mRNA vaccine? 01:11:13 - 'Side-effects of vaccine same as covid' 01:12:57 - Why are mRNA vaccines being employed now? 01:13:57 - How can a person protect themselves from an mRNA vaccine? 01:16:54 - Lies, Damned Lies and Health Statistics – the Deadly Danger of False Positives by Dr. Michael Yeadon 01:17:27 - Endotoxin, TLR-4, tetracyclines, vaccines 01:19:14 - Ingredients of the Pfizer vaccine (lipid adjuvants) 01:22:01 - The great reset and a Biden presidency, CFR, CIA 01:26:21 - World economic forum and cyber polygon cybersecurity event 01:31:50 - China, Russia, the great reset, AI 01:36:04 - Chinese electromagnetic pulse weapons 01:37:44 - Is AI overrated? 01:43:01 - Covid-19 cured the flu 01:44:47 - When did the education system in the U.S. become insane? 01:47:57 - What is Ray working on right now? 01:49:43 - Hyperthermia therapy 01:54:10 - Estrogen and cortisol tend to decreases the temperature 01:57:22 - Methylene blue and red light 02:00:36 - Email raypeatsnewsletter@gmail.com for Ray's newsletter subscription and books
00:00 - Setup music 08:14 - Start and catch up 10:45 - Other life in the universe? 16:38 - Process theology, radical empiricism 18:19 - Ideologues in cosmology 20:10 - Halton Arp, Frederick Soddy 22:11 - N.A. Kozyrev 24:06 - H.C. Dudley, neutrino sea 26:04 - Where is matter from? 28:09 - David Bohm 29:22 - Do humans create matter? 32:08 - Sensory deprivation experiments 32:31 - What do you see when you close your eyes? 41:02 - Are serotonin and melatonin necessary for dreaming? 42:46 - Melatonin in trauma 43:28 - Symbols of an Alien Sky documentary by David Talbott, Vladimir Vernadsky, Immanuel Velikovsky 45:40 - Cosmic Clocks by Michel Gauquelin, H.C. Dudley, nuclear decay 53:02 - Is carbon dating accurate? 54:58 - Is nuclear power inherently dangerous? 56:30 - Did advanced civilizations exist? 57:44 - Coral Castle by Edward Leedskalnin 58:43 - Could humanity solve its own problems if there was no authoritarian influence? 59:36 - Does Ray remember a time when there was more liberty? 01:00:27 - Was there a natural factor that contributed to the current increase in evil? 01:02:00 - Evil is like glycolysis 01:02:39 - Klaus Schwab and His Great Fascist Reset https://off-guardian.org/2020/10/12/klaus-schwab-his-great-fascist-reset/ 01:07:22 - Crimes against humanity by Reiner Fuellmich 01:08:25 - The legal system a way to interfere with the great reset? 01:09:33 - The military is involved in every vaccine trial, biological warfare, China 01:17:08 - "The media prevents you from knowing how you're being dominated, by keeping the CIA's secrets. The media and the CIA are same thing." Douglas Valentine 01:18:55 - Adverse events in vaccine trials 01:20:11 - What causes polio? 01:23:10 - Endotoxin and Guillain-Barré syndrome 01:24:04 - Is aluminum more dangerous than endotoxin as an adjuvant? 01:24:56 - The physiology of being offended 01:27:24 - Low metabolism and subordination 01:28:46 - Body Pleasure and The Origins of Violence James W. Prescott (1975) 01:32:25 - The weaponization of sex 01:33:36 - "The use of noncommunicative jargon can be an indication of a personality disorder." Ray Peat 01:35:32 - What can shift a person away from authoritarianism? 01:36:36 - When did blue lights replace incandescent lights in hospitals? 01:38:22 - Plastics, static discharge, toxic chemicals in plastic 01:40:36 - In defense of Mexican coke: sucrose, coca leaf extract, caffeine, 4-methylimidazole, potassium content 01:47:13 - Fresh sweet fruit juice preferable to coke, puritanical nutritionism 01:49:00 - Pepsi vs. coke 01:50:00 - "When people supplement thyroid and eat liver once or twice a week, their acne and dandruff (and many other problems) usually clear up very quickly..." Ray Peat (2011) 01:52:05 - How many "grains" of thyroid is in a cynoplus tablet? 01:58:55 - Vaccination against rabies virus 02:02:13 - Pregnenolone contamination
Learning Objectives Discuss the importance of low levels of endotoxins in parenteral preparations Discuss the limits for endotoxins for various routes of administration Discuss ways to control the levels of endotoxins in final preparations In this weeks' 483 we're going to be talking about Bacterial Endotoxins and the testing related to endotoxins. Let's first take a look at the observation then we'll discuss what endotoxins are and why it's important to keep them out of your preparations. First thing that should be talked about specific to this 483 is that we're dealing with preparations that are intrathecals or will be injected into the spinal fluid. One thing to know about intrathecal injections in particular is that they have a lower limit for endotoxins than other injections. When performing final testing for preparations one of the tests is a bacterial endotoxin test. This test is performed according to USP Chapter Bacterial Endotoxins Test. For parenterals that are not intended for intrathecal administration the limit is 5 EU/kg (Endotoxin Units). For intrathecal injections the limit is 0.2 EU/kg. The per kilogram is the weight of the patient. The fact that there is a much lower limit of endotoxin specifically for intrathecal injections should give an indication as to the importance of having very low levels of endotoxin in the final preparation. This particular pharmacy is probably compounding the intrathecal in either very low batch sizes (less than 5) or one at a time as they're ordered by a physician. By not batch compounding the intrathecals there's not enough of the final preparation to send off for testing. My guess is that these are being made one at a time per order and are delivered to be administered within 24 hours. What are Bacterial Endotoxins and where do they come from? Most common, endotoxins are by-products of the gram negative bacterial cell lysis. They are lipopolysaccharides that make up part of the cell wall. Gram negative bacteria are commonly found in water sources. So if at any point you are using water either in the preparation itself or using materials that have been washed (i.e. glassware), there is a very high risk of having bacterial endotoxins in the preparation if there aren't any controls in place to limit the level of bacterial endotoxins. If glassware is being washed and re-used for processing the preparation, the glassware should go through a depyrogenation cycle meaning they would be held at a temperature of 250 degrees celsius for a minimum of 30 minutes. The time may vary depending on the glassware and oven that's being used for the depyrogenation process. Depyrogenation cycles of glassware should be validated to show that the process of depyrogenation is producing a 3-log reduction in bacterial endotoxins. Even if the process for limiting endotoxins in glassware in place you still need to consider any of the raw materials you're using (i.e. the active pharmaceutical ingredient or API). Water is used quite frequently in the manufacturing of pharmaceutical ingredients and the certificates of analysis for your API should include the limit for endotoxin and the results of testing for endotoxins. It's pointed out in this 483, that the certificates of analysis (COAs) do not include results of endotoxin limits for the APIs. Why are Endotoxin limits so critical? When a sufficient amount of endotoxins are injected this could result in a fever or can be as severe as death; particularly if injected into the spinal fluid where there is no immune system. Since this pharmacy doesn't have COAs that show the endotoxin results and are not testing for endotoxin in their final preparation, they really have no way of showing that their intrathecal injections are meeting the required limits for endotoxins. There are several ways that this pharmacy could increase the level of assurance that their process is limiting the endotoxin in the final preparation. According to USP Chapter there are 3 ways of controlling endotoxin in parenteral products: Indirect Control, "which is comprised of a series of preventive measures that control bioburden, the potential endotoxin contribution by formulation components (e.g., raw materials, APIs, excipients), water, primary packaging components, equipment, and the manufacturing environment, including personnel." Process Control, "in which endotoxin is monitored at Critical Control points (CCP) during processing to ensure that there is no increase in endotoxin. These process control elements are subject to validation or qualification." Direct Control, "or the direct destruction or removal of endotoxins from product streams, equipment, and primary packaging materials. As with controls on processing, direct measures of endotoxin destruction or removal must be validated." First, you'd need to evaluate the process for compounding the preparation. At any point is water being used in the process (i.e. glassware and other materials for compounding)? If so, are you using the proper process to control endotoxins? Are there ways to possibly eliminate the need for glassware and materials that may have endotoxins; could single use depyrogenated and sterilized materials and equipment be used? Lastly, is there a way to compound the intrathecals in batches so that a percentage of the batch is sent off for testing to increase the level of assurance that endotoxins are at low enough limits for the injectable being compounded. References FDA Inspection Technical Guide: Bacterial Endotoxins/Pyrogens USP Chapter Bacterial Endotoxins Test Associates of Cape Cod BET White Paper: ENDOTOXIN LIMITS For Parenteral Drug Products USP Chapter Depyrogenation USP Chapter Dry Heat Depyrogenation USP Chapter Depyrogenation by Filtration USP Chapter Depyrogenation by Rinsing
Learning Objectives Discuss the importance of low levels of endotoxins in parenteral preparations Discuss the limits for endotoxins for various routes of administration Discuss ways to control the levels of endotoxins in final preparations In this weeks' 483 we're going to be talking about Bacterial Endotoxins and the testing related to endotoxins. Let's first take a look at the observation then we'll discuss what endotoxins are and why it's important to keep them out of your preparations. First thing that should be talked about specific to this 483 is that we're dealing with preparations that are intrathecals or will be injected into the spinal fluid. One thing to know about intrathecal injections in particular is that they have a lower limit for endotoxins than other injections. When performing final testing for preparations one of the tests is a bacterial endotoxin test. This test is performed according to USP Chapter Bacterial Endotoxins Test. For parenterals that are not intended for intrathecal administration the limit is 5 EU/kg (Endotoxin Units). For intrathecal injections the limit is 0.2 EU/kg. The per kilogram is the weight of the patient. The fact that there is a much lower limit of endotoxin specifically for intrathecal injections should give an indication as to the importance of having very low levels of endotoxin in the final preparation. This particular pharmacy is probably compounding the intrathecal in either very low batch sizes (less than 5) or one at a time as they're ordered by a physician. By not batch compounding the intrathecals there's not enough of the final preparation to send off for testing. My guess is that these are being made one at a time per order and are delivered to be administered within 24 hours. What are Bacterial Endotoxins and where do they come from? Most common, endotoxins are by-products of the gram negative bacterial cell lysis. They are lipopolysaccharides that make up part of the cell wall. Gram negative bacteria are commonly found in water sources. So if at any point you are using water either in the preparation itself or using materials that have been washed (i.e. glassware), there is a very high risk of having bacterial endotoxins in the preparation if there aren't any controls in place to limit the level of bacterial endotoxins. If glassware is being washed and re-used for processing the preparation, the glassware should go through a depyrogenation cycle meaning they would be held at a temperature of 250 degrees celsius for a minimum of 30 minutes. The time may vary depending on the glassware and oven that's being used for the depyrogenation process. Depyrogenation cycles of glassware should be validated to show that the process of depyrogenation is producing a 3-log reduction in bacterial endotoxins. Even if the process for limiting endotoxins in glassware in place you still need to consider any of the raw materials you're using (i.e. the active pharmaceutical ingredient or API). Water is used quite frequently in the manufacturing of pharmaceutical ingredients and the certificates of analysis for your API should include the limit for endotoxin and the results of testing for endotoxins. It's pointed out in this 483, that the certificates of analysis (COAs) do not include results of endotoxin limits for the APIs. Why are Endotoxin limits so critical? When a sufficient amount of endotoxins are injected this could result in a fever or can be as severe as death; particularly if injected into the spinal fluid where there is no immune system. Since this pharmacy doesn't have COAs that show the endotoxin results and are not testing for endotoxin in their final preparation, they really have no way of showing that their intrathecal injections are meeting the required limits for endotoxins. There are several ways that this pharmacy could increase the level of assurance that their process is limiting the endotoxin in the final preparation. According to USP Chapter there are 3 ways of controlling endotoxin in parenteral products: Indirect Control, "which is comprised of a series of preventive measures that control bioburden, the potential endotoxin contribution by formulation components (e.g., raw materials, APIs, excipients), water, primary packaging components, equipment, and the manufacturing environment, including personnel." Process Control, "in which endotoxin is monitored at Critical Control points (CCP) during processing to ensure that there is no increase in endotoxin. These process control elements are subject to validation or qualification." Direct Control, "or the direct destruction or removal of endotoxins from product streams, equipment, and primary packaging materials. As with controls on processing, direct measures of endotoxin destruction or removal must be validated." First, you'd need to evaluate the process for compounding the preparation. At any point is water being used in the process (i.e. glassware and other materials for compounding)? If so, are you using the proper process to control endotoxins? Are there ways to possibly eliminate the need for glassware and materials that may have endotoxins; could single use depyrogenated and sterilized materials and equipment be used? Lastly, is there a way to compound the intrathecals in batches so that a percentage of the batch is sent off for testing to increase the level of assurance that endotoxins are at low enough limits for the injectable being compounded. References FDA Inspection Technical Guide: Bacterial Endotoxins/Pyrogens USP Chapter Bacterial Endotoxins Test Associates of Cape Cod BET White Paper: ENDOTOXIN LIMITS For Parenteral Drug Products USP Chapter Depyrogenation USP Chapter Dry Heat Depyrogenation USP Chapter Depyrogenation by Filtration USP Chapter Depyrogenation by Rinsing
00:00 - Setup music 02:46 - Skip setup music 14:09 - These live streams are now on Spotify 15:07 - An update on Idealabs research and development 19:08 - Navel application/absorption rivals intravenous route, other skin routes not far behind 23:55 - Doxycycline akin to Meldonium / Mildronate, may cure triple-negative breast cancer 29:09 - Endotoxin (LPS) can trigger social withdrawal / isolation 37:22 - Estrogen directly causes polycystic ovary syndrome (PCOS) 45:26 - Progesterone – not a sex hormone, but a systemic health hormone 50:37 - Many “inert” additives in drugs and food are actually active and toxic 57:42 - Super chat: treating a cough from mold 59:51 - Super chat: different hormones receptor relationships 01:06:58 - Super chat: foamy urine and glucuronidation 01:11:55 - Super chat: reducing iodine in the body? 01:14:23 - Super chat: A1 vs. A2 milk 01:16:20 - Super chat: can the ear lobe crease be reversed? 01:17:55 - Super chat: safely supporting the metabolism in late age 01:24:54 - Super chat: thoughts on keratosis pilaris 01:27:38 - Super chat: less energy when eating throughout the day 01:31:19 - Super chat: why does coffee cause inflammation? 01:35:29 - Super chat: why is living at high altitude beneficial? 01:42:21 - Super chat: pregnanolone in food? 01:49:01 - Super chat: lutein and zeaxanthin for eye health? 01:51:28 - Super chat: how do Danny and Georgi use thyroid? 01:57:41 - Super chat: how does Georgi prefer to take vitamin D? 02:01:14 - Super chat: high heart rate with standing up? 02:03:09 - Super chat: what is the best way to take 20 mg of progesterone? 02:05:19 - Super chat: fluctuations in testicle size? 02:08:54 - Super chat: does methylene blue have teratogenic effects? 02:11:50 - Super chat: what brand of calcium does Georgi use?
00:08:43 - Skip setup music 00:10:05 - Sinus related issues, endotoxin, allergens in food 00:13:03 - My supplementation routine 00:15:56 - Can a person overdo sugars in the beginning? 00:18:26 - Is nizoral (ketoconazole) safe? 00:19:45 - Danny's own experience with antibiotics 00:28:43 - Shampoo for hair loss 00:29:18 - Randle cycle, food combining, oral health, SIBO, testosterone, pulse and temperature, lipid peroxidation, the bioenergetic elevator pitch, iodine 00:42:13 - Oysters how many times a week? The importance of zinc 00:44:03 - Problems with milk? What are the benefits of fasting? 00:49:39 - Danny's experience EMF 00:53:00 - Thoughts on supplemental digestive enzymes 00:55:03 - Oral health, vitamin K, vitamin D, disinfecting the intestine, CO2 00:56:12 - Bacterial and fungal issues despite good nutrition 00:58:54 - Is stinging nettle useful for hair loss? 00:59:33 - Concentration difficulties, feeling "keyed up" 01:00:32 - Hormones, smell, and taste 01:01:57 - So-called "orthorexia" 01:04:21 - Interconnection between hair loss and libido problems, testosterone, prolactin, hypothyroidism 01:07:34 - Antibiotic resistance, evolutionary foods, amino acid recycling 01:10:41 - What's Danny's life like in Thailand? 01:12:43 - Thoughts on the safety of Mexico 01:16:36 - The effects of living at high altitude 01:17:34 - Muscles as fatty acid sinks 01:18:27 - The bioenergetic view of heavy metal detox 01:21:55 - Vegetarian alternative to liver, exercise, food additive gums, working with a traditional MD 01:24:07 - Proteolytic enzymes for scalp fibrosis? 01:24:34 - Correcting the hypercarotenemia of hypothyroidism 01:26:05 - Danny's opinion of Stephanie Buttermore's "all in" approach to health 01:27:33 - A bioenergetic view of paralysis 01:27:45 - Danny's thoughts on Weston A. Price 01:31:04 - Danny's thoughts on food combining 01:32:06 - Any way to mitigate Tourettes? 01:32:17 - Water loading, prolactin, libido, hypothyroidism 01:35:39 - Why do some people experience hair loss while others do not? 01:38:42 - Can phytoestrogens reduce estrogen? 01:38:17 - Supplemental creatine 01:40:20 - Alopecia areata, vitamin D 01:42:15 - Vitiligo, finasteride, estrogen, food for kids 01:43:22 - What does Danny think about inversion tables? 01:43:34 - Does no one have any success on the bioenergetic approach to pattern baldness? 01:51:28 - Varicocele, nitric oxide 01:52:40 - Danny hasn't experimented with his casein yet 01:54:28 - Danny's thoughts on the changing society
On Episode 3 of the Musashi R&D Sessions Podcast, Ross and Dhiren discuss the psychological and emotional benefits of physical activity and much more in this week's topic – Mental Health & Exercise. For this session we are joined by Luvern Moothan, a Mental Health Clinician from his office in Perth, to dissect the effect exercise on the mind and brain, as well as the body. The guys also elaborate on the science of neurotransmitters, with a focus on serotonin, which plays a significant role in mood and happiness, and dopamine, which influences feelings of pleasure and reward. While most sporting fixtures are on hold, the guys speak about several competitions which continue to run, including the Belarusian Premier League, and review the news of Liverpool seeking to have the Premier League title awarded to them in court. More news surfaces around Jon Jones' arrest, Lamelo Ball buying the Illawarra Hawks, and Christian Welch calling out to seniors, among other stories. This episode we finish with a pair of listener questions about progressive overloading, and consuming protein before a workout. R&D are more successful in this week's attempt to guess the origin of a famous athlete's quote pitched by Hal the Intern. Once again, Dhiren applies the philosophy of Miyamoto Musashi to the topic using the quote: "Think lightly of yourself and deeply of the world". Remember to comment and leave a review, and we'll get in touch to send you a free Musashi T-shirt! For more product information and training tips, visit www.musashi.com Scientific References: 1. Neurotransmitters in Depression and Anxiety: https://www.frontiersin.org/articles/... 2. Exercise and Tryptophan-Serotonin link: https://journals.plos.org/plosone/art... 3. Endotoxin and behaviours: https://journals.plos.org/plosone/art... 4. The role of inflammation and the gut microbiome in depression and anxiety https://onlinelibrary.wiley.com/doi/1... 5. Injecting pro-inflammatory cytokines causes anxiety: https://www.jneurosci.org/content/32/... 6. Gut bacteria transplant in mice and effect on anxiety/depression: https://www.ncbi.nlm.nih.gov/pubmed/3... Mental Health Support Resources: e-Mental Health Programs: myCompass www.mycompass.org.au myCompass is an interactive self-help service that aims to promote resilience and wellbeing for all Australians. MoodGYM www.moodgym.anu.edu.au MoodGYM is a free self-help program to teach CBT skills to people vulnerable to depression and anxiety. THIS WAY UP www.thiswayup.org.au Using CBT principles, THIS WAY UP Clinic offers proven online courses for Depression, GAD, and Anxiety. MindSpot www.mindspot.org.au MindSpot combines educational and practical exercises with regular contact with a MindSpot therapist, allowing help to be obtained in a discreet, effective way. Apps and other resources: ReachOut Breathe app https://au.reachout.com/tools-and-app... ReachOut Breathe helps you reduce the physical symptoms of stress and anxiety by slowing down your breathing and your heart rate. Smiling Mind app https://www.smilingmind.com.au Smiling Mind is a meditation app that uses mindfulness to help manage stress, resilience, anxiety, depression and improve general health and wellbeing. Black Dog Snapshot https://www.blackdoginstitute.org.au/... Black Dog Snapshot is an app that allows you to measure and monitor mental health and lifestyle factors that influence wellbeing.
00:10:52 - Skip Setup Music 00:19:01 - What is knowledge? 00:22:07 - "...I think you have to start and end with the experience of being an animal in the world." RP (2019) 00:27:36 - Pavlov's first- and second-signal system 00:32:51 - Declining societal health 00:37:30 - Excessive verbalization 00:41:32 - Recreating your history 00:45:32 - "The study of vaccines, 'vaccinology,' in isolation from the developmental effects of nutrition and environmental stress, is a pseudoscience, blind to the mechanisms that produce harmful long range effects." Contexts for Vaccinations by Ray Peat, PhD (2020) 00:50:19 - PUFA, viral infections, and immunity 00:52:58 - Prasad A., et al. Role of calcium and ROS in cell death induced by polyunsaturated fatty acids in murine thymocytes. J Cell Physiol. 2010 Nov;225(3):829-36. "An increase in intracellular calcium is the first event to occur.” "These results suggest that the six PUFAs we studied kill thymocytes by causing release of calcium from endoplasmic reticulum, which causes release of ROS from mitochondria which leads to cell death.” "It is perhaps surprising that essential lipids that are supposed to be so good for health are so potent at killing cells." 00:54:09 - "Herd immunity" 00:56:52 - Immunity, cancer, and endotoxin 01:00:45 - Warburg effect, lactic acid, and cancer 01:02:45 - Endotoxin, antibiotics, cancer, medical culture, PMS 01:08:07 - The use of cortisol and morphine for cancer 01:09:10 - Danny way too loud and Paul Ehrlich 01:10:29 - Cell receptors as "sense organs" 01:14:42 - Resurgence of estrogen HRT 01:18:33 - We lose Ray and call him back 01:20:30 - Ray is back 01:22:41 - Estrogen, reductive stress, and cancer 01:23:00 - What is Ray's motivation? What is his general view of health and sickness? 01:28:26 - Ray is following in the tradition of Albert Szent-Györgyi, Otto Warburg, William F. Koch, J.C. Bose, etc. 01:30:09 - Sick animals and people tend to retreat... 01:33:04 - Is there a way forward with the current political system? 01:40:20 - Has an empire ever been reformed? 01:46:10 - "The reason I talk more about biology than politics is that the various radical movements generally have inflexibilities that keep them apart. People need to start understanding that the system is systematically murdering them, and understanding that the situation is desperate, they need to see that solidarity with life, against capital, is their hope." Raymond Peat, PhD (2018) 01:48:07 - Organizing the panic interview with Ray Peat 01:55:43 - Consciousness as a basic property of matter 02:01:55 - Mitochondrial metabolism and resonance 02:06:55 - Rupert Sheldrake and morphic resonance 02:09:35 - David Bohm and creation 02:13:15 - What is time?
Endotoxin? Playful energy? In this conversation with Georgi Dinkov, he talks about what endotoxin (lipopolysaccharide) is, how high endotoxic load can cause serious problems, how endotoxemia manifests itself down stream, and what we can do about it - all under the umbrella of biological energy and living in a way that doesn't suck. Read more of Georgi's digging up, here: http://haidut.me/ Here a few links / research Georgi mentioned during the conversation: https://raypeatforum.com/community/threads/damaged-mitochondria-and-endotoxin-lps-drive-chronic-inflammation.28621/ http://haidut.me/?p=660 http://haidut.me/?p=141 https://raypeatforum.com/community/threads/blocking-endotoxin-lps-may-reverse-cure-diabetes-type-i.29149/
Dr. Raymond Peat is back to discuss all things related to digestion, acid reflux, GERD, gas and bloating and more. Here are the time stamps: Time Stamps: 1:10 Why are digestive problems seemingly worse than ever? 5:12 The North To South Process of Digestion 7:59 Why you need Stomach Acid 12:24 Liver and Gallbladder Issues 15:38 What is Endotoxin and why is it dangerous? 16:40 The Gallbladder And why you have bad breath 21:59 Symptoms of High Levels of Endotoxins 23:59 Acid Reflux, a symptom of endotoxin 27:05 Anxiety and the gut-brain connection 29:36 Dr Peats thoughts Prebiotics and Probiotics 37:20 What Dr Peat says about the health of poo 38:40 Foods recommended for good digestion 42:32 What foods to eliminate if you have digestive issues 48:30 Lifestyle advice for good digestion 51:30 How you can support Dr Peat for all his hard work and generous knowledge. To support Dr. Peat's work, please sign up for his newsletter: raypeatsnewsletter@gmail.com To learn more about Dr. Peat, visit: http://www.raypeat.com To learn more about Jodelle, visit: http://www.getfitwithjodelle.com Thank you to our show sponsors: http://www.swanwicksleep.com and use code: GETFIT for 10% off http://www.dropanfbomb.com and use code: GETFIT for 10% off Thank you for your support, and please LIKE, SHARE, AND SUBSCRIBE!
Dr. Raymond Peat is back to discuss all things related to digestion, acid reflux, GERD, gas and bloating and more. Here are the time stamps: Time Stamps: 1:10 Why are digestive problems seemingly worse than ever? 5:12 The North To South Process of Digestion 7:59 Why you need Stomach Acid 12:24 Liver and Gallbladder Issues 15:38 What is Endotoxin and why is it dangerous? 16:40 The Gallbladder And why you have bad breath 21:59 Symptoms of High Levels of Endotoxins 23:59 Acid Reflux, a symptom of endotoxin 27:05 Anxiety and the gut-brain connection 29:36 Dr Peats thoughts Prebiotics and Probiotics 37:20 What Dr Peat says about the health of poo 38:40 Foods recommended for good digestion 42:32 What foods to eliminate if you have digestive issues 48:30 Lifestyle advice for good digestion 51:30 How you can support Dr Peat for all his hard work and generous knowledge. To support Dr. Peat's work, please sign up for his newsletter: raypeatsnewsletter@gmail.com To learn more about Dr. Peat, visit: http://www.raypeat.com To learn more about Jodelle, visit: http://www.getfitwithjodelle.com Thank you to our show sponsors: http://www.swanwicksleep.com and use code: GETFIT for 10% off http://www.dropanfbomb.com and use code: GETFIT for 10% off Thank you for your support, and please LIKE, SHARE, AND SUBSCRIBE!
The interview starts at 32:50 Brandon Powell joins us to chat about The Wim Hof breathing method, shamanism and training your mind, spirit and body. Brandon is one of the few official Wim Hof trainers so we go deep into that breathing method and what led Brandon to that point. We also chat about shamanism, martial arts, the health aspects of the cold thermogenesis, the flow state, Qi-gong, the archetypal sense, Love and peak experience. https://www.wimhofmethod.com/instructors/brandonpowell https://www.instagram.com/brandonpowell396/ https://www.facebook.com/DojoBodyMind/ http://dojobodymind.com/ In the intro, we chat about the hidden teachings of Jesus youtube video - and Jesus being a Yogi?!?!? some listener synchronicities, and of course the deep state quotes. See the links below for stuff we chatted about during the show and the intro: https://www.youtube.com/watch?v=Upj5U1jx_WA&list=PL20LFUx2HFw6fPeFmHI6tfXc-k5Y4BCbq The Hidden Teachings of Jesus https://www.jcf.org/ https://www.spiritofchi.com/program/metaphysics/gtumo-metaphysics/gtumo-tibetan/ http://www.elizabethbader.com/elizabethbadersblog/stephen-porges-polyvagal-theory-healing-trauma/ Please help support the show…. Grimerica’s DoBeDoBeDo List: Grimerica is fully and solely listener supported. We adhere to the Value for Value model. 0 ads, 0 sponsorships, 0 breaks, 0 portals and links to corporate websites… just many hours of unlimited content for free. Thanks for listening!! https://www.13questionspodcast.com/ Our New Podcast - 13 Questions Join the chat / hangout with a bunch of fellow Grimerican’s www.grimerica.ca/chats 1-403-702-6083Call and leave a voice mail or send us a text Support the show directly http://www.grimerica.ca/support https://www.patreon.com/grimerica GrimericaFM https://s2.radio.co/s053ed3122/listen Check out our next trip/conference/meetup - Contact at the Cabin Leave a review on iTunes and/or Stitcher https://itunes.apple.com/ca/podcast/the-grimerica-show/id653314424?mt=2# http://www.stitcher.com/podcast/the-grimerica-show Sign up for our newsletter http://www.grimerica.ca/news Leave a comment, ideas and guest/topic suggestions under any episode or blog http://www.grimerica.ca/ SPAM Graham = and send him your synchronicities, feedback, strange experiences and psychedelic trip reports!! graham@grimerica.com InstaGRAM https://www.instagram.com/the_grimerica_show_podcast/ Tweet Darren https://twitter.com/Grimerica Connect through other platforms: https://www.reddit.com/r/grimerica/ https://gab.ai/Grimerica Purchase swag, with partial proceeds donated to the show www.grimerica.ca/swag Send us a postcard or letter http://www.grimerica.ca/contact/ Thanks to Wayne Darnell for help with the website. http://www.darnelldigitalink.com/ http://www.lostbreadcomic.com/ link to Napolean Duheme's site Felix’s Site sirfelix.bandcamp.com Christmas Carol Video MUSIC Grimerica Theme - Lock & Key Murmur - Broke for Free sirfelix.bandcamp.com
This episode was edited from its original format to comply with anchor's 3-hour limit.
-----Criador_do_Podcast----- Prof. Nilton Vivacqua Gomes • Especialista, Mestre e Doutor em Endodontia pela UNICAMP. • Professor dos Cursos de Aperfeiçoamento, Especialização e Imersão em Endodontia da ABO-CE (Coordenador), ELEVA-CE e FAMETRO-CE. • Professor-Coord. do Mestrado de Excelência em Endodontia da Faculdade SL Mandic-CE. • Professor de Endodontia do Mestrado em Clínica Odontológica da FACPP-CE • Professor Convidado dos Cursos de Aperfeiçoamento e Especialização do IOA-Balneário Camboriú-SC. ----------------------------------------- -----Cursos----- Clique nos Links abaixo para: Curso VIP: http://bit.ly/vivacquavip Aperf. e Imersões: http://bit.ly/aperfeic Especializações: http://bit.ly/especializ Materiais - Dentalbox Conceito (85) 98180-9804 ----------------------------------------- -----Apoio----- Você pode apoiar essa iniciativa, para incentivá-la a continuar indefinidamente. É só acessar www.apoia.se/ENDOdontoCast e escolher o valor com o qual deseja apoiar mensalmente. Vale até mesmo 1 real. Quanto maior o valor, maior o benefício. -----Apoiadores----- Categoria Ouro: Indianara Chinaqui Categoria Prata: Sandro Rodrigues Pinheiro Ana Paula Santos Flavia Darius Vivacqua ----------------------------------------- -----Podcast_Apps----- Podcasts (Nativo), Overcast, Breaker (IOS), Google Podcasts (Android), Acast, Ivoox, Wecast, Castbox, Spotify, PocketCasts (IOS/Android). Instale o app e na aba busca e procure pelo ENDOdonto Cast. Inscreva-se clicando no botão destinado a isso. Selecione a config. p/ baixar novos episódios automaticamente. ----------------------------------------- -----Referências_Científicas----- Quantification of endotoxins and cultivable bacteria in root canal infection before and after chemomechanical preparation with 2.5% sodium hypochlorite. Martinho et al. J Endod, 2008 Comparison of 2.5% Sodium Hypochlorite and 2 % Chlorhexidine Gel on Oral Bacterial Lipopolysaccharide Reduction from Primarily Infected Root Canals. Gomes et al. J Endod, 2009. Efficacy of endodontic treatment for endotoxin reduction in primarily infected root canals and evaluation of cytotoxic effects. Oliveira et al. J Endod, 2012. One-visit versus two-visit root canal treatment: effectiveness in the removal of endotoxins and cultivable bacteria. Xavier et al. J Endod, 2013. Monitoring the effectiveness of root canal procedures on endotoxin levels found in teeth with chronic apical periodontitis. Marinho et al. J Appl Oral Sci, 2014. Proinflammatory Activity of Primarily Infected Endodontic Content against Macrophages after Different Phases of the Root Canal Therapy. Marinho et al. J Endod, 2015. Endotoxin levels after chemomechanical preparation of root canals with sodium hypochlorite or chlorhexidine: a systematic review of clinical trials and meta-analysis. Neelakantan et al. Int End J, 2019. ----------------------------------------- -----Site_e_Contatos----- EndodontiaAvancada.com EndodontiaAvancada.com@gmail.com EndodontiaAvancada.com@hotmail.com ----------------------------------------- -----Redes_Sociais----- Instagram/Facebook: NiltonVivacqua Página/Grupo do Facebook: EndodontiaAvancada.com --- Send in a voice message: https://anchor.fm/endodontiaavancada/message
Integrate Yourself Podcast | Integrated Fitness & Nutrition | Healthy Lifestyle & Personal Growth
Episode 60: IN THIS EPISODE WE DISCUSS:-The difference between antimicrobial fibers and undigestible fibers and how including antimicrobial fiber can be more beneficial to your gut health which relates to your metabolism.-The benefits of the daily raw carrot salad-The components of the raw carrot salad and the role they play in the digestive system-How the carrot salad aids in hormonal balance, detoxification and improving digestion.BENEFITS OF A DAILY CARROT SALAD INCLUDE:Aids the liver in the detoxification process by carrying the toxins away via the carrot fiberStimulates the digestionIncreases thyroid hormone conversionDecreases stress hormones; adrenaline and cortisol, as well as, aldosterone and serotoninRelieves premenstrual symptomsContains an indigestible fiber that's antimicrobial and antibacterial which pull out endotoxins (bad bacteria) in the gut. Making your gut flora a much healthier place.When you cut your carrot lengthwise (even after you chew it) you get the benefit of it pulling out endotoxin much more effectively and lower in the digestive tract.The raw carrot is effective at detoxing excess estrogen in the gut and prevent estrogen from getting reabsorbed in the gut, thus creating hormonal balance.Eat your carrot salad between meals, between breakfast and lunch or lunch and dinner. BENEFITS OF ESSENTIAL COMPONENTS OF THE DAILY CARROT SALADThe coconut oil or olive oil aids in offsetting the unsaturated fat in the carrot as well as stimulates the metabolism by lowering your requirement for vitamin E, suppressing bacterial overgrowth and healing the intestinal wall. The vinegar aids in digestion and stimulating HCL. The salt aids in keeping adrenaline, aldosterone and serotonin levels down, stimulates the bowel, provides minerals your body needs, increases metabolism, maintains a healthy blood sugar and makes your salad taste better.Subscribe:https://www.youtube.com/user/PureEner...Carrot Salad Blog:https://www.pureenergypdx.com/blog/carrot-salad-recipeAccess show notes, comment and share at:https://www.pureenergypdx.com/integrate-yourself-podcast/bacterial-overgrowth4-Sigmatic affiliate link:http://www.us.foursigmatic.com/#_a_in...Enter our code and get 10% off: integrateyourselfJoin us on Patreon:http://www.patreon.com/integrateyourselfSupport the show (https://www.patreon.com/integrateyourself)
This week Bryan and I discuss the differences between endotoxin and endospores; diving a little deeper into the post from last week. We talk about the basics of these two, why they're important and how to kill or deactivate them. WE'RE NOW ON iHEART RADIO PODCASTS (click here)! Sign up for updates from PharmacyInspection.com: [mc4wp_form id="14886"] If you like the podcast follow us on twitter @PharmacyInspect and share the podcast with those who might find it helpful. We're just getting warmed up; there's SO many more topics to cover! Stay tuned for what's to come! Thanks for listening. Do you have a question or comment for the show? Leave us a VOICEMAIL, right on our site! --- Support this podcast: https://anchor.fm/pharmacy-inspection/support
In today's episode, Dr Nirala Jacobi welcomes back Dr Jason Hawrelak, to talk about Microbiome Restoration. Dr Hawrelak is a researcher, educator, naturopath, and nutritionist with over 16 years of clinical experience. He also practices at Gould's Natural Medicine - a 135-year-old natural medicine apothecary and clinic in Hobart, Tasmania, Australia. Dr Hawrelak completed his PhD examining the capacity of probiotics, prebiotics, and herbal medicines to modify the gastrointestinal tract microbiota. He is the senior lecturer in Complementary and Alternative Medicines at the University of Tasmania's School of Medicine where he coordinates the evidence-based complementary medicines programs. Dr Hawrelak also teaches the gastrointestinal imbalances unit, within the Masters of Science and Human Nutrition, and Functional Medicine Program at the University of Western States, in Portland, Oregon. Topics discussed include: The SIBO Doctor courses Microbiome Restoration - coming soon Regrowing vs killing in the microbiome. Colonic collateral when we are dealing with SIBO, and the necessity to restore the colonic ecosystem as part of the SIBO treatment. How do we learn to interpret the DNA readings and PCR reports? The SIBO Doctor Microbiome Restoration course A discussion of the modules offered in the Microbiome Restoration course Module 1 - Testing The difference between different labs Culturing vs DNA techniques Assessment, Interpretation, and Diagnosis Module 2 - Beneficial Bacteria, including Akkermansia Faecalibacterium prausnitzii Butyrate-producing microbes Hydrogen sulfide gas producers Module 3 - Pathobionts The good, the bad, the ugly Hydrogen sulfide producers Methanobrevibacter smithii coli Bacteroides Triple antibiotic therapy (for conditions such as H. Pylori and Blastocystis Hominis) and the impact on the colonic ecosystem balance of symbionts and pathobionts. Dietary changes vs probiotic supplements to elicit change in the gut microbiome. The complications between advising prebiotic nutrition for optimising microbiome restoration, whilst clients are on therapeutic diets such as the Low FODMAP diet - how to reconcile? Hydrogen sulfide breath testing - coming soon. High-fat content diets in SIBO, and how this can eventually feed hydrogen sulfide producing bacteria. Symptoms such as food reactivity and visceral sensitivity as a consequence of dietary choices in SIBO treatment, in regards to hydrogen sulfide producing bacterial blooms. The therapeutic benefits of Soy Isoflavones in relation to Methane and Hydrogen Sulfide producing SIBO patients. Equol producers - patients who have bacteria that convert soy isoflavones into the bioactive component, Equol. Adlercreutzia levels in people who eat soy products as a long-term dietary choice. Is there a connection between histamine intolerance, salicylate intolerance, and oxalate intolerance and microbiome disturbances? Post SIBO treatment food sensitivities - the colonic microflora patterns to observe. Hydrogen sulfide gas and how it causes visceral sensitivity, gut leakiness, and inflammation in the nerves. Dietary changes with people with sensitivities - a reiteration of start low, go slow. Is there any clinical significance to oxalobacter formigenes being elevated? What is the dietary impact of the extinction of oxalobacter formigenes? Testing levels of Proteobacteria to uncover the innate endotoxin load (proteobacteria - gram-negative bacteria that secrete proinflammatory endotoxins). The systemic repercussions of endotoxemia - leaky blood-brain barrier, gut damage, insulin sensitivity impacts, systemic inflammation, Alzheimer's, anxiety and depression links. Endotoxin (also known as Lipopolysaccharides - LPS) absorption and high-fat How do different Phyla respond to different diets? Potential outcomes of the ketogenic diet depending on the dietary nuances - decreased microflora diversity, increased hydrogen sulfide gas-producing bacteria, and increased numbers of proteobacteria. Akkermansia and constipation - why are these often seen together? Gut inflammation and mucus tend to increase Akkermansia if it is there - it is a mucin eater. Gut markers of inflammation, such as calprotectin. The vocabulary to use around probiotics - not re-seeding, but rather restoring. The importance of lactic and acetic acid production adjusting the pH of our microbiome to be hospitable to beneficial bacteria. D-Lactate considerations. Biogaia effectively reducing methane production, and also being used alongside proton pump inhibitors (PPIs) to prevent the development of SIBO. PPIs - when to use and when not to - listen to Dr Steven Sandberg-Lewis' podcast on SIBO and Functional GIT Exam Skills. Plantarum LP8 is being used in research to decrease Desulfovibrio, increase butyrate and bifidobacterium - can we use other strains of L. Plantarum to elicit similar changes? Plantarum strains to use in gut rehabilitation. Reuterin production. Dr Hawrelak's antimicrobial and dietary recommendations for treating Desulfovibrio. Resources Dr Hawrelak Probiotic Advisor Gould's Natural Medicine The SIBO Doctor Microbiome Restoration Course with Dr Jason Hawrelak [Opens 13 June] Sign Up for the Course and Live Q & A with Dr Hawrelak The SIBO Conference New Orleans - speakers mentioned Dr Richard McCallum Dr Matthew Bohm Labs mentioned Ubiome - microbiome DNA testing Genova Dr Satish Rao SIBO and comorbid SIFO D-Lactic Acidosis researcher Dr Steven Sandberg-Lewis Dr Steven Sandberg-Lewis' podcast on SIBO and Functional GIT Exam Skills
This week Seth DePasquale and Bryan Prince discuss the differences between endotoxin and endospores; diving a little deeper into the post from last week. Seth & Bryan talk about the basics of these two, why they’re important and how to kill or deactivate them. https://pharmacyinspection.com/pharmacy-inspection-podcast-episode-20-endotoxin-vs-endospores/ CONTACT the SHOW: https://pharmacyinspection.com/contact/
This week Seth DePasquale and Bryan Prince discuss the differences between endotoxin and endospores; diving a little deeper into the post from last week. Seth & Bryan talk about the basics of these two, why they're important and how to kill or deactivate them. https://pharmacyinspection.com/pharmacy-inspection-podcast-episode-20-endotoxin-vs-endospores/ CONTACT the SHOW: https://pharmacyinspection.com/contact/ See omnystudio.com/listener for privacy information.
This week Bryan and I discuss the differences between endotoxin and endospores; diving a little deeper into the post from last week. We talk about the basics of these two, why they're important and how to kill or deactivate them. WE'RE NOW ON iHEART RADIO PODCASTS (click here)! Sign up for updates from PharmacyInspection.com: [mc4wp_form id="14886"] If you like the podcast follow us on twitter @PharmacyInspect and share the podcast with those who might find it helpful. We're just getting warmed up; there's SO many more topics to cover! Stay tuned for what's to come! Thanks for listening. Do you have a question or comment for the show? Leave us a VOICEMAIL, right on our site!
This week Bryan and I discuss the differences between endotoxin and endospores; diving a little deeper into the post from last week. We talk about the basics of these two, why they're important and how to kill or deactivate them. WE'RE NOW ON iHEART RADIO PODCASTS (click here)! Sign up for updates from PharmacyInspection.com: [mc4wp_form id="14886"] If you like the podcast follow us on twitter @PharmacyInspect and share the podcast with those who might find it helpful. We're just getting warmed up; there's SO many more topics to cover! Stay tuned for what's to come! Thanks for listening. Do you have a question or comment for the show? Leave us a VOICEMAIL, right on our site!
Ep. 235: Denise Minger and Extreme Diets In this episode, Stacy and Sarah invite Denise Minger, author of Death By Food Pyramid, on to talk about research she did on extremely high carb, extremely low fat diets and why they seem to help heal people with serious disease, contrary to conventional paleo wisdom. Click the picture above to be taken to iTunes If you enjoy the show, please review it in iTunes! The Paleo View (TPV), Episode 235: Denise Minger and Extreme Diets Intro (0:00) News and Views (0:40) Our guest is Denise Minger of DeniseMinger.com! She's here to answer a specific question we received about a talk she did at AHS '14! He book Death By Food Pyramid is one of Sarah's favorites! See the podcast we did with her about it here. Question from Valerie: "first of all I'd like to thank you for what you're doing. Your show always puts a smile on my face and I am afraid of the day I will have caught up with all past episodes and will actually have to wait a week for the next one to come out. So I was ordering my InstantPot the other day when Amazon recommend I shall buy the book "The Starch Solution" by John McDougall. I got curious about it and did some research and it seems the diet is a paleo person's worst nightmare: Based solely on starches, whole grains, legumes, no fats allowed whatsoever, zero animal products. It promises weight loss (not hard to imagine, being on a totally bland diet), but also permanent reversal (as in healing) of diabetes and all sorts of health benefits. I also found a talk by Denise Minger ('lessons from the vegans', I think you might actually have mentioned it sometime) who confirms these findings. What's puzzling me is all the reports of people doing that diet talking about improved digestion, no blood sugar spikes (!) and I also found quite a few reports from Crohn's patients apparently being cured by it, although this is totally counter intuitive for anyone that has ever been on SCD or paleo. Can you think of any explanation for these miraculous cures?" Denise posted a post about this topic here. A study by Walter Kempner found that a super high sugar, super low fat diet had benefits for people with diabetes, multiple sclerosis, heart disease and other conditions. He was trying to help people with kidney failure. 96% carbohydrate and mostly white sugar, about 2000 calories. What came out of this came to be known as the Rice Diet Look at the end of these notes for OFFICIAL Denise Minger meme images. These benefit lasted even after they returned to a normal diet. Roy Swank found that people in mountains of Norway were eating a lot of land animal fats and had a relatively high incidence of multiple sclerosis while people eating mostly fish on the coast had a low incidence of MS. He put people with MS on a very low saturated fat diet for decades and found a lot of success with the people who stuck the closest to his diet. This doesn't seem to jibe with what we know of MS, particularly compared with people like Terry Wahls who eat much more fat. Nathan Pritikin worked on a plant-based, super low fat diet to reverse heart disease. With all this very thorough research that seems contradictory to the paleo model, it seemed to Denise like she needed to revise what she believed was true. Denise initially thought it was the low processed foods component with mainly whole foods focus that was the cause. But obviously the Kempner mostly refined sugar diet that doesn't apply. But it seems like these extreme diets have evidence that they can help you repair when you're sick, but not necessarily to prevent the disease in the first place! Denise found that most low fat diets call for 30% of calories from fat, while these extreme diets are under 10%. The really problematic diets are the refined grain diets with that 30% or more calories from fat that have the most disease promoting properties. Denise believes that this is because of the gut and how chylomicrons and endotoxins (lipopolysaccharides) work on these diets. Endotoxins are linked to a lot of modern diseases and are present the most in high saturated fat diets. Chylomicrons are lipoproteins in the gut that transport fat out to the rest of the body, but they will also bring endotoxins along with them. To reduce endotoxins in the blood, you can either eat this super low fat diet, or you can promote a healthy gut that doesn't produce a lot of endotoxins to begin with. For diabetes, saturated fat increases the secretion of insulin. This is why a sugar diet could improve diabetes. There could be a fat/carbohydrate interaction in some people that may make them more susceptible to blood sugar issues. It seems that these plant based diets are so adamant about super low fat: if you even eat a moderate amount of fat, the therapeutic effect goes away! Denise concludes that healthy people without these conditions shouldn't be worried about her research because these diets are for therapeutic effect and don't have evidence that they benefit people trying to prevent disease. Endotoxin is created by the cell walls of gram-negative bacteria. Gram-negative bacteria are sensitive to the fiber intake of the diet. Jeff Leach of the Human Food Project studies the human gut microbiome. Read his post Going Feral: My One-Year Journey to Acquire the Healthiest Gut Microbiome in the World (You Heard Me!) to get the story of him testing his own gut microbiome and figuring out what variables helped improve it. Endotoxin is highly inflammatory. It's how we get lab rats to develop disease and it is the cause of sepsis. Thanks to Denise for coming on the show on short notice! Go to DeniseMinger.com for everything about her! And read her book Death by Food Pyramid! Outro (43:18) Support us by shopping through links on our sidebars, please!
In this episode, we delve into the relationship between Candida Albicans and SIBO. Our host, Dr Nirala Jacobi, is in conversation with Dr Margaret Beeson. Dr Beeson has over 40 years in medical practice, having started as a nurse in the US Navy and then moving to become a Naturopathic physician. She founded the Yellowstone Naturopathic Clinic in Billings, Montana, and is the President and Co-founder of the Naturopathic Education and Research Consortium to increase post graduate training in the USA. Dr Beeson is also the president of the Paul Gardener Veterans Pain Relief Foundation, and is the recipient of 2016 Physician of the Year award from American Association of Naturopathic Physicians. She is a national leader in efforts to advance the quality of naturopathic care. Topics discussed in this episode include: - Small intestinal fungal overgrowth (SIFO) and large intestinal fungal overgrowth in relation to candidiasis. - The comorbidity relationship of SIFO to SIBO. - Endotoxin association with SIFO. - The metamorphic abilities of candida and its ability to hide in the body, including candida antibodies versus counter antibodies. - Candida as a symptom of dysbiosis. - Biofilm and candida living as a part of diverse bacterial colonies rather than solitary candida colonies. - Yeasts and their pathogenic potential. - Microbiome terrain disturbance as a key player in candida infection progression. - Die off as stronger with fungus than with bacteria. - Candidal hyphae, the mucosal wall, and the potential for leaky gut. - Hallmark symptoms of candida. - The 4 forms of candida, and which is the most invasive and detrimental. - Testing and symptomatic identification of candidiasis. - Organic acid testing and candidiasis diagnosis. - Fibromyalgia, tartaric acid, and the candidiasis connection. - Saccharomyces boulardii tolerance and/or use during yeast infection. - Reactions to kombucha and why they might occur. - Folliculitis as a symptom of yeast and staph cohabitation infection. - Nystatin (nilstat), how it works, safety profile, affordability, treatment options, length of treatment, and recommended daily unit intake. - The role of biofilm disruptors in treatment. - Candida as a contributor to salicylate sensitivity. - Dr Jacobi's SIBO specific treatment herbs and how they can also address candida. - Saccharomyces boulardii and its adherence action to encourage friendly flora. - Specific prebiotics, whole food diets, and their role in repopulating microflora diversity in the gut. - Dr Beeson's complementary protocols and therapies to consider during candida treatment. - Is candida albicans a trigger in the onset of coeliac disease? Resources Dr Margaret Beeson ○ Yellowstone Naturopathic Clinic, Billings, Montana Probiotics Dr Beeson uses ○ Human Lactic Commensals from Farmax. Dr Anderson and biofilm Dr Jason Hawrelak, probiotic expert, speaks at the SIBO summit
Endotoxin in Asthma: Friend or Foe?
Endotoxin in Asthma: Friend or Foe?
Endotoxin in Asthma: Friend or Foe?
Infertility - Causes and Treatments from a Functional Medicine Perspective [ctt title="Infertility solutions via Functional Medicine" tweet="#FunctionalMedicine solutions for #infertility " coverup="5Gu2U"] Infertility has many causes that mainstream medicine never addresses. Unless you investigate the possible causes and address what you find, you have no hope of a successful pregnancy. In this episode, we will cover multiple under-investigated causes of infertility and discuss how to address them. Issues addressed are thyroid function, autoimmunity, birth control, gut health, and more. Hear what the latest research has to say on these causes of infertility.
Medizinische Fakultät - Digitale Hochschulschriften der LMU - Teil 16/19
Thu, 28 Nov 2013 12:00:00 +0100 https://edoc.ub.uni-muenchen.de/16413/ https://edoc.ub.uni-muenchen.de/16413/1/Haider_Daniel.pdf Haider, Daniel ddc:610, dd
Abbott, N (University of Wisconsin-Madison ) Thursday 13 June 2013, 16:00-17:30
Treatment of acute lung injury (ALI) remains an unsolved problem in intensive care medicine. As simvastatin exerts protective effects in inflammatory diseases we explored its effects on development of ALI and due to the importance of neutrophils in ALI also on neutrophil effector functions. C57Bl/6 mice were exposed to aerosolized LPS (500 µg/ml) for 30 min. The count of alveolar, interstitial, and intravasal neutrophils were assessed 4 h later by flow cytometry. Lung permeability changes were assessed by FITC-dextran clearance and albumin content in the BAL fluid. In vitro, we analyzed the effect of simvastatin on neutrophil adhesion, degranulation, apoptosis, and formation of reactive oxygen species. To monitor effects of simvastatin on bacterial clearance we performed phagocytosis and bacterial killing studies in vitro as well as sepsis experiments in mice. Simvastatin treatment before and after onset of ALI reduces neutrophil influx into the lung as well as lung permeability indicating the protective role of simvastatin in ALI. Moreover, simvastatin reduces the formation of ROS species and adhesion of neutrophils without affecting apoptosis, bacterial phagocytosis and bacterial clearance. Simvastatin reduces recruitment and activation of neutrophils hereby protecting from LPS-induced ALI. Our results imply a potential role for statins in the management of ALI.
Two related ligands that activate the same receptor have different effects on immune system stimulation and inflammation.
Medizinische Fakultät - Digitale Hochschulschriften der LMU - Teil 10/19
Thu, 22 Oct 2009 12:00:00 +0100 https://edoc.ub.uni-muenchen.de/10722/ https://edoc.ub.uni-muenchen.de/10722/1/Schenkat_Sonja.pdf Schenkat, Sonja
Medizinische Fakultät - Digitale Hochschulschriften der LMU - Teil 09/19
Thu, 22 Jan 2009 12:00:00 +0100 https://edoc.ub.uni-muenchen.de/9583/ https://edoc.ub.uni-muenchen.de/9583/1/Listle_Holger.pdf Listle, Holger
Medizinische Fakultät - Digitale Hochschulschriften der LMU - Teil 06/19
Landwirte sind durch ihr Arbeitsumfeld einer erhöhten Konzentration von organischem Staub, insbesondere Endotoxinen im Stall, bei erhöhtem Lungenminutenvolumen durch körperliche Arbeit ausgesetzt. Ziel der vorliegenden Untersuchung war es, unter Berücksichtigung der Endotoxinkonzentrationen das broncho-pulmonale System auf Entzündungsreaktionen zu untersuchen. Insbesondere war hier die nasale Lavage von Interesse, da hierbei durch eine nicht-invasive Untersuchungstechnik Rückschlüsse auf den Zustand des Respirationstraktes gezogen werden können. Zum Vergleich und um Aussagen über systemische Entzündungsreaktionen zu erhalten, wurden ausgewählte Entzündungsparameter im peripheren, venösen Blut quantitativ bestimmt. Die Untersuchung erfolgte an zwei Kollektiven gesunder Probanden. Es wurden 21 Landwirte mit 24 nicht beruflich exponierten Personen verglichen und das Auftreten von respiratorischen und allergischen Symptomen nach Exposition erfasst. Des Weiteren wurden eine Lungenfunktionsuntersuchung, eine Blutabnahme und eine Nasallavage zur Evaluierung der häufigsten Blut- und Entzündungsparameter durchgeführt. Im Plasma wurden die proinflammatorischen Zytokine TNF-, IL-6 und ENA-78 bestimmt. Zusätzlich wurde Endotoxin personenbezogen gemessen sowie die Kohlendioxid- und Ammoniakkonzentrationen im Stall ortsfest bestimmt. Temperatur, Luftfeuchtigkeit und Windgeschwindigkeit im Stall wurden vor Ort am Expositionstag ermittelt. Die gemessenen Endotoxin Konzentrationen lagen im Bereich von 0,9 EU/m3 bis 20816,3 EU/m3. Die Lungenfunktionswerte zeigten eine signifikante Erhöhung über die Stallarbeit. VCinsp (vor Expo: 107,7 ± 3,7 %; nach Expo: 112,4 ± 3,7 %; pw=0,039), FEV1 (vor Expo:111,2 ± 4,0 %; nach Expo: 114,6 ± 3,8 %; pw=0,042), und MEF50 (vor Expo: 82,0 ± 7,5 %; nach Expo: 88,1 ± 6,2 %; pw=0,044) stiegen signifikant an. Die Analyse des Differentialblutbildes zeigte das Bild einer Entzündungsreaktion nach Exposition. Es erhöhte sich signifikant der Anteil der neutrophilen Granulozyten an den Leukozyten (vor Expo: 52,7 ± 1,6 %; nach Expo: 62,9 ± 1,6 %; pw=0,003). Lymphozyten fielen von 35 ± 1,56 % auf 27,5 ± 1,3 % ab (pw=0,003), eosinophile Granulozyten fielen von 3,5 ± 0,4 % auf 7,3 ± 0,3 % (pw=0,013), Monozyten von 8,4 ± 0,5 % auf 7,3 ± 0,3 % (pw=0,012), CRP von 0,2 ± 0,04 mg/dl auf 0,15 ± 0,04 mg/dl (pw=0,011). Auch in der Kontrollgruppe kam es zu einer ähnlichen, ebenfalls signifikanten Veränderung, diese war jedoch geringer ausgeprägt. Für die Zytokine im Serum zeigte sich keine statistisch signifikante Veränderung über die Exposition. Jedoch ergab sich ein positiver Zusammenhang zwischen der IL-6 Konzentration im Serum und der Höhe der Endotoxinbelastung im Stall. Dieser Zusammenhang war allerdings nicht statistisch signifikant (p=0,056). Bei Betrachtung der Zelldifferenzierung der Nasallavage konnte ein geringer, aber signifikanter Abfall der Anteile der neutrophilen Granulozyten (vor Expo: 88 ± 5,7 %; nach Expo: 77 ± 8,7 %; pw=0,047) festgestellt werden. Außerdem ergab sich ein hochsignifikanter Zusammenhang zwischen der Höhe der Endotoxinkonzentration und der nasalen Makrophagen (psp=0,0001), sowie der nasalen Epithelzellen (psp=0,022). Im Hinblick auf unsere Anfangs gewählte Fragestellung deutet unser Ergebnis an, dass eine Exposition gegenüber organischem Staub, insbesondere Endotoxin, zu einer Neutrophilie im Serum führen kann und bei hohen inhalierten Endotoxinkonzentrationen auch vermehrt Makrophagen in der Nasenschleimhaut rekrutiert werden. Da sowohl die Lungenfunktionsparameter, als auch die systemischen (Serum) und lokalen (Nasallavage) Entzündungszeichen das Bild einer Entzündungsreaktion zeigen, kann davon ausgegangen werden, dass die Lungenfunktion, sowie die systemischen und lokalen Messmethoden über ausreichende Sensitivität verfügen, um in diesem Studiendesign Entzündungsreaktionen nachzuweisen. Die Nasallavage konnte im Feldversuch als eine objektive, kostengünstige, schnelle und gut tolerable Messmethode zur Darstellung von nasalen Entzündungsreaktionen etabliert werden.
Medizinische Fakultät - Digitale Hochschulschriften der LMU - Teil 04/19
Das „Acute Respiratory Distress Syndrome“ (ARDS) ist eine akut auftretende, überwiegend Sepsis-induzierte, inflammatorische Erkrankung der Lunge mit hoher Letalität. Ein komplexes Netzwerk aus proinflammatorischen Zytokinen und Mediatoren initiiert und perpetuiert dabei die pulmonale Entzündungreaktion. Vor diesem Hintergrund steht das Konzept der therapeutischen Suppression dieser Substanzen. Dem Zytokin Interleukin-10 (IL-10) könnte in diesem Zusammenhang aufgrund seines ausgeprägten antiinflammatorischen Wirkspektrums eine Bedeutung zukommen; IL-10 hemmt physiologisch die Synthese und Freisetzung von Entzündungsmediatoren. In tierexperimentellen Untersuchungen bei Sepsis bzw. experimenteller Endotoxinämie konnte die protektive Wirkung von systemisch appliziertem IL-10 auf das Überleben der Versuchstiere sowie die Verminderung der Konzentrationen zirkulierender, proinflammatorischer Mediatoren gezeigt werden. Mit der vorliegenden Studie wurde erstmals untersucht, welche Auswirkungen eine Behandlung mit inhaliertem IL-10 im Hinblick auf die pulmonale und systemische Entzündungsreaktion hat. Da die Tierversuche in der intubierten und kontrolliert beatmeten Ratte durchgeführt werden sollten, musste zunächst ein hierfür geeignetes Verneblersystem entwickelt werden. Die in vitro Evaluation des neu entwickelten Verneblersystems ergab, dass das entwickelte Jetverneblersystem eine für eine alveoläre Deposition geeignete Partikelgröße liefert (d = 2µm). In vivo zeigten die produzierten Aerosolpartikel eine gleichmässige Deposition über die gesamte Lunge. Insgesamt deponierten rund 3.8%(1.3) (Median(IQR)) der vernebelten Ausgangslösung in alveolären Bereichen. Der Vernebelungsprozess beeinflusste weder die Herzfrequenz, den mittleren arteriellen Blutdruck, den arteriellen Sauerstoff- und Kohlendioxidpartialdruck noch die Integrität des Lungenparenchyms und kann daher als sicher angesehen werden. An 24 narkotisierten, kontrolliert beatmeten Ratten wurde die antiinflammatorische Wirkung von IL-10-Aerosol untersucht. Die Induktion des experimentellen Lungenschadens erfolgte durch intravenöse Injektion von Endotoxin (LPS). Die Tiere wurden zufällig einer von drei Versuchsgruppen zugeordnet: Die LPS-Gruppe erhielt eine LPS-Injektion (5mg/kg/KG) ohne therapeutische Intervention. Bei der IL-10-Gruppe erfolgte unmittelbar vor LPS-Injektion eine Behandlung mit IL-10-Aerosol (vernebelte Dosis: ~5μg/Tier; deponierte Dosis: ~0.19 μg/Tier). In einer Kontrollgruppe wurde die Auswirkung von Narkose, chirurgischer Präparation, Beatmung und Aerosolapplikation (Inhalation von PBS als die IL-10-Trägerlösung) evaluiert. Während einer Beobachtungszeit von 6h nach LPS-Injektion wurden kontinuierlich die Hämodynamik und Lungenmechanik sowie stündlich die arteriellen Blutgase und das Butbild bestimmt. Am Ende der Versuche erfolgten eine Bronchoalveoläre Lavage (BAL) sowie die Entnahme von Blut- bzw. Plasmaproben. Wichtigste Zielparameter waren hierbei die pulmonale und systemische Entzündungsreaktion. Durch die Injektion von LPS konnte sowohl systemisch als auch pulmonal eine akute Entzündungsreaktion ausgelöst werden. Zwar führte die experimentelle Endotoxinämie nur zu geringen Verschlechterungen der klinischen Parameter, jedoch zeigte sich sowohl in der BAL als auch im Plasma ein Anstieg der bestimmten proinflammatorischen Mediatoren (TNF-α, IFN-γ, RANTES, IL-1, IL-6). Desweiteren führte LPS zu einer Freisetzung reaktiver Stickstoffradikale aus durch die BAL gewonnenen und ex vivo kultivierten Alveolarmakrophagen. Die Applikation von IL-10-Aerosol senkte die erhöhten Konzentrationen von IFN-γ, RANTES, IL-1 und IL-6 sowohl in der BAL als auch im Plasma. Eine Reduktion der TNF-α-Konzentration war nur in der BAL nachweisbar. Auch die Freisetzung von reaktiven Stickstoffradikalen aus Alveolarmakrophagen wurde durch IL-10-Inhalation suffizient supprimiert. Die Vernebelung von IL-10 vor Induktion einer experimentellen Endotoxinämie zeigte sowohl pulmonal als auch systemisch antiinflammatorische Wirkungen. Basierend auf den hier vorgestellten Befunden können nun weitere Untersuchungen zur Beschreibung der Dosis-Wirkungsbeziehung und zur Bestimmung des Zeitfensters für eine therapeutische Applikation von IL-10-Aerosol nach LPS-Stimulation folgen.
Medizinische Fakultät - Digitale Hochschulschriften der LMU - Teil 03/19
Das biologische Geschlecht wird als ein Risikofaktor für die Entstehung und den Verlauf von septischen Zustandsbildern kontrovers diskutiert. In der hier vorgelegten Arbeit werden die Einflüsse genetischer Faktoren unter Berücksichtigung des biologischen Geschlechts und der Wirkung von Sexualsteroiden auf die entzündliche Antwort in einem Tiermodel untersucht. Hierzu wurde genotypisch verschieden Mausstämmen (A/J, C57BL6/J, DBA/2J, BALB/cJ und AKR/J) bakterielles Endotoxin (Lipopolysaccharid, LPS) intraperitoneal injiziert. Als Ausdruck der entzündlichen Antwort wurden Plasmaspiegel der Zytokine Tumor Nekrose Faktor alpha (TNF-a) und Interleukin 10 (IL-10) mittels ELSIA gemessen. Es zeigten sich deutliche geschlechtsspezifische Unterschiede in der LPS-induzierten entzündlichen Antwort von C57BL6/J-Mäusen (=B6), die hormonell bedingt zu sein scheinen. Der Vergleich mit A/J-Mäusen weist hier jedoch eine unterschiedliche Ausprägung und somit eine Abhängigkeit von genetischen Faktoren auf. Außerdem lässt sich die entzündliche Antwort durch Gabe von Sexual-Steroiden modulieren. Hierzu wurden Mäuse kastriert bzw. ovariektomiert und vor der Injektion von LPS mit 17-ß-Estrogen oder 5-a-Dihydrotestosteron behandelt. Männliche Tiere reagierten dabei allerdings deutlich besser auf diese Beeinflussung von außen. Darüber hinaus sind diese Effekte abhängig von genetischen Faktoren. Nach Änderung der hormonellen Bedingungen fanden sich bei den Männchen der einzelnen Stämme deutliche Unterschiede: Während sich einige Stämme z.T. unbeeinflusst, wie z.B. die IL-10 Plasmaspiegel von DBA/2J und BALB/c Mäusen nach Östrogen-Behandlung zeigten, fanden sich bei andere Stämme gar gegensätzliche Antworten, so z.B: die TNF-a Plasmaspiegel von A/J und B6 Mäusen nach Östrogen-Behandlung. Untersuchungen an der F1-Generation von A/J und B6 Mäusen zeigten, dass die beobachteten Effekte unabhängig von den Geschlechtschromosomen oder genetischem Imprinting zu sein scheinen. Die beobachteten Veränderungen durch hormonelle Manipulation wurden auch in ihrer Auswirkung auf den Verlauf nach einer letalen LPS-Injektion untersucht. Da Androgene allgemein als hauptsächlich verantwortlich für geschlechtsspezifische Unterschiede der entzündlichen Antwort eingeschätzt werden, wurde von der Verringerung der systemischen Androgenspiegel ein Überlebensvorteil erwartet. Um so interessanter war die Beobachtung, dass diesbezüglich lediglich A/J Mäuse nach chirurgischer Kastration vor den Auswirkungen von LPS geschützt waren. Dieser protektive Effekt könnte die Folge von sexual-steroid-abhängigen Änderungen in der Relation von pro- zu anti-inflammatorischer Komponente der entzündlichen Antwort sein. Es ist anzunehmen, dass dieser Schutz nur bei entsprechender genetischer Konstellation und wahrscheinlich in Abhängigkeit vom Verletzungsmechanismus zustande kommt. Östrogen-Behandlung von männlichen A/J and B6 Mäusen brachte kein verbessertes Überleben nach Endotoxinschock. Die vorgelegten Daten erlauben die Schlussfolgerung, dass biologisches Geschlecht und individuelle genetische Ausstattung gemeinsam einen messbaren Einfluss auf die LPS-induzierte entzündliche Antwort haben. Könnte man diese Ergebnisse auf Menschen übertragen, so ließe sich hieraus eine Erklärung für gegensätzliche Beobachtungen bei geschlechtsspezifischen Unterschieden in klinischen Studien ableiten. Genetische Marker könnten helfen, die Einflüsse des biologischen Geschlechts auf die entzündliche Antwort klinisch besser untersuchen zu können. Die Suche nach solchen Markern sollte in Zukunft intensiviert werden, da ihnen auch eine große Bedeutung für das Design von Laborexperimenten und klinischen Studien zukommt, aus denen sich dann eventuell sogar therapeutische Ansätzen zur Milderung der sekundären Effekte von Verletzungen ableiten lassen.
Medizinische Fakultät - Digitale Hochschulschriften der LMU - Teil 02/19
1. Bei Patienten mit FL kommt es in der Akutphase der Erkrankung zu einer neutrophilen Alveolitis. In der Lavageflüssigkeit dieser Patienten fanden sich im Vergleich zu gesunden Normalpersonen erhöhte Spiegel an IL-8. Die Lavageflüssigkeit hatte eine verstärkte chemotaktische Aktivität gegenüber PMNs im Vergleich zur Lavageflüssigkeit von gesunden Kontrollpersonen. Nach Neutralisation von IL-8 durch Zugabe von Anti- IL-8-Antikörpern konnte ein signifikanter Rückgang der Chemoattraktion von PMNs durch die Lavageflüssigkeit festgestellt werden. 2. Es fand sich eine signifikante Korrelation zwischen der chemotaktischen Aktivität der Lavageflüssigkeit auf PMNs sowohl mit den in der Lavageflüssigkeit enthaltenen Endotoxinspiegeln als auch den in der Lavageflüssigkeit enthaltenen Spiegeln an IL-8. 3. Nach Stimulation mit Heustaub reagierten Alveolarmakrophagen mit der Sekretion von TNF-alpha, IL-6 und IL-8. Aus diesen Ergebnissen lässt sich folgendes Modell für die Pathogenese des akuten Schubs der FL ableiten: Im Heustaub enthaltenes Endotoxin stimuliert nach Inhalation Alveolarmakrophagen zur Sekretion von proinflammatorischen Zytokinen. Diese führen zu einer systemischen und lokalen Entzündungsreaktion. Sezerniertes IL-8 bewirkt eine neutrophile Alveolitis. Die von aktivierten PMNs ausgeschütteten Proteasen greifen das Strukturgerüst der Lunge an und stören die strukturelle Integrität der Lunge. Durch den in der Folge einsetzenden Regenerationsprozess mit Proliferation von Fibroblasten kommt es zur Ausbildung einer Lungenfibrose.
Medizinische Fakultät - Digitale Hochschulschriften der LMU - Teil 01/19
Thu, 16 Jan 2003 12:00:00 +0100 https://edoc.ub.uni-muenchen.de/765/ https://edoc.ub.uni-muenchen.de/765/1/Eichhorn_Martin.pdf Eichhorn, Martin E.
Medizinische Fakultät - Digitale Hochschulschriften der LMU - Teil 01/19
Fragestellung: Um mögliche proinflammatorische Zeichen bzw. Frühindikatoren (sog. Mikroinflammation) bei Patienten mit chronischem Nierenversagen und nierentransplantierten Patienten aufzuspüren, die an späteren Organkomplikationen (chronische Transplantatdysfunktion, Herz-Kreislaufmorbidität, endotheliale Dysfunktion, Infektanfälligkeiten) beteiligt sein können, untersuchten wir die Expression funktioneller monozytärer Oberflächenantigene. Als immunologischen Marker der Aktivität einer Entzündungsreaktion verglichen wir die in der Durchflusszytometrie (fluorescence-activated cell sorter (FACS))gemessene Expression sog. mCD14-Rezeptoren und der CD14+CD16++-Rezeptoren auf peripheren Blut-Monozyten mit serologischen Parametern wie C-reaktives Protein und Leukozytenzahl. Die Frage, ob die beiden Oberflächenantigene CD14 und CD16 sowie die Koexpression beider Antigene (proinflammatorisch aktivierte Monozyten) zum „immunologischen Monitoring“ geeignet sind, sollte erstmalig klinisch an Gesunden, Patienten nach Nierentransplantation sowie Patienten mit chronischer Niereninsuffizienz im Endstadium ohne Dialysebehandlung untersucht werden. Ergänzend wurden nichtorgantransplantierte Patienten mit akuten infektiösen Komplikationen auf mögliche Modulationen proinflammatorischer Blutmonozyten (CD14+/CD16++-Zellen) hin untersucht. CD14 existiert membrangebunden (mCD14) und in löslicher Form (sCD14) und ist verantwortlich für die Interaktion von Endotoxin (LPS) mit Monozyten und Neutrophilen. LPS ist ein Glykoprotein der äußeren Membranschicht gramnegativer Bakterien. CD14 ist der wichtigste Rezeptor für gram-negative bakterielle Lipopolysaccharide (Endotoxin), jedoch auch für Peptidoglykan und Lipoteichonsäure gram-positiver Erreger. CD 16 ist der funktionell wichtige Fcγ–Rezeptor Typ III (FcγRIII, IgG-Rezeptor Typ III), der mit niedriger Affinität monomeres IgG sowie polymeres IgG oder Immunkomplexe bindet. Er vermittelt wichtige immunphysiologische Funktionen wie antikörperabhängige zellvermittelte Zytotoxizität, Beseitigung zirkulierender Immunkomplexe, Superoxidbildung und ist auch an der Signaltransduktion beteiligt. Die untersuchten CD14+CD16++-Monozyten bewirken im Zusammenwirken von TNFα, IL-1, IL-6 und IL-10 bewirkt eine stärkere Entzündungsreaktion als die konventionellen CD14++CD16negativen-Zellen; sie sind sehr potente Antigen-präsentierende Zellen, besitzen eine hohe Phagozytoseaktivität und verstärken die endotheliale Adhäsion. Untersuchungsaufbau: Wir untersuchten 69 Patienten nach Nierentransplantation (23 Frauen, 46 Männer) im Alter von durchschnittlich 52,63 +/- 11,42 Jahren (Median 53,84 Jahre). Die Patienten erhielten durchschnittlich vor 6,38 +/- 5,2 Jahren ein Nieren- Transplantat (Median 8,16 Jahre). Wir unterschieden diese Patienten hinsichtlich ihrer Immunsuppression in folgende Gruppen: 1) Mycophenolat-Mofetil-Monotherapie (MMFmono), 2) Cyclosporin-Monotherapie (CyAmono) und 3) einer Kombination aus MMF und CyA (MMF-CyAkombi). In die Gruppe mit MMF-Monotherapie wurden16 Personen eingeschlossen (3 Frauen, 13 Männer) im Alter von durchschnittlich 51,29 +/- 10,47 Jahren (Median 51,15 Jahre). In der Gruppe der CyA-monotherapierten Patienten befanden sich 11 Personen (3 Frauen, 8 Männer) im Durchschnittsalter von 58,43 +/- 8,01 Jahren (Median 59,96 Jahre). Zur Gruppe der MMF-CyA-kombinationstherapierten Patienten gehörten 18 Patienten (3 Frauen, 15 Männer) im Alter von 46,69 +/- 10,22 Jahren (Median 47,44 Jahre). Des weiteren wurden 13 Patienten mit chronischer Niereninsuffizienz (12 Männer, eine Frau) im Alter von 32 bis 74 Jahren (Median 65,0 Jahre; Mittelwert 63,5 Jahre +/- 10,3 Jahre) in die Analysen miteinbezogen. Parallel wurden 8 Patienten (6 Frauen, 2 Männer) im Verlauf einer akuten Infektion erfasst (Mittelwert 74,6 +/- 10,53 Jahre; Median 78 Jahre). Unser Kontrollkollektiv bestand aus 18 klinisch gesunden freiwilligen Probanden im Alter zwischen 24 und 54 Jahren (Median 31Jahre; Mittelwert 33,42 +/- 9,91 Jahre; 10 Frauen, 8 Männer). Ergebnisse: 1. Patienten mit chronischer Niereninsuffizienz hatten signifikant höhere CRP-Serumkonzentrationen als Gesunde (p=0,010)(Gesunde 0,33 mg/dl vs. Urämiker 0,7 mg/dl). 2. Die mCD14-Expression auf peripheren Blutmonozyten war bei Urämikern (p=0,024) und bei nierentransplantierten Patienten (p=0,026) signifikant niedriger als bei Gesunden.(Gesunde 517 MFI vs. Urämiker 426 MFI vs. NTX 433 MFI) 3. Der prozentuale Anteil CD14+CD16++-Monozyten im Blut war sowohl bei Patienten mit chronischer Niereninsuffizienz (p=0,00000487; MFI= 13,0%) als auch bei Patienten nach Nierentransplantation (p=0,00298; MFI=9,3%) signifikant höher. 4. Die Leukozytenzahl im Blut war weder bei immunsupprimierten Nierentransplantierten noch bei Patienten mit chronischer Niereninsuffizienz im Endstadium signifikant gegenüber Gesunden verändert. 5. Hinsichtlich der verschiedenen Strategien der immunsuppressiven Therapie ließen sich keine Unterschiede zwischen den Gruppen der MMF-mono, der CyA-mono und der MMF-CyA-kombi-behandelten Nt-Patienten feststellen. 6. Die Absolutzahl CD14+CD16++-Monozyten war sowohl bei urämischen Patienten (p=0,003; 430/µl) als auch bei nierentransplantierten Patienten (p=0,005; 413/µl) gegenüber Gesunden signifikant erhöht. 7. Bei Nierentransplantierten fiel die CRP-Konzentration im Serum mit steigendem Alter des Transplantates logarithmisch ab (p=0,065), die mCD14-Expression fiel linear ab (p=0,063) wohingegen der prozentuale Anteil der CD14+CD16++- Monozyten invers ansteigt (p=0,0036). 8. Im Verlauf akuter infektiöser Erkrankungen war der Anteil der CD14+CD16++- Monozyten signifikant höher als bei Gesunden (p=0,000049) und fiel bis zur stationären Entlassung so signifikant ab (p=0,012), so dass kein Unterschied mehr zwischen Gesunden und Kranken mehr nachweisbar war. Schlußfolgerungen: 1. Sowohl bei Patienten mit chronischer Niereninsuffizienz wie bei Nierentransplantierten finden sich anhand erhöhter Zahlen proinflammatorischer Blutmonozyten (CD14+CD16++-Phänotyp) eindeutig Zeichen einer sog. „Mikroinflammation“; dies ausdrücklich auch bei NTX-Patienten, obwohl diese unter einer dauerhaften immunsuppressiven Behandlung stehen. 2. Proinflammatorische Blutmonozyten sind Ziel- und Effektorzellen der Immunabwehr. Darüber hinaus sind sie pathophysiologisch an den Vorgängen einer Atheromatose beteiligt. CD14+CD16++-Blutmonozyten entsprechen dem Phänotyp von Gewebsmakrophagen. Erhöhte Anteile CD14+CD16++-Blutmonozyten gehen möglicherweise parallel mit der erhöhten Progressionsrate der Atheromatose von Organtransplantierten und Patienten mit Niereninsuffizienz. Andererseits sind sie an der Auslösung und Perpetuation der chronischen Transplantatdysfunktion (chronisches Transplantatversagen) ursächlich beteiligt, was aus Biopsiedaten hervorgeht. Die hier erfolgten Blutzellanalysen unterstützen diese These. 3. Dauerimmunsuppression bei Nierentransplantierten vermag nicht den proinflammatorischen Status diese Patienten zu unterdrücken. Damit gilt für alle NTXPatienten, dass sich mehr oder weniger schnell trotz potenter Immunsuppression irgendwann ein Transplantatversagen einstellt. Es spricht alles dafür, dass aktivierte Blutmonozyten hier die Schlüsselrolle spielen. Wir postulieren, dass dies nur dann abgeschwächt oder vermindert werden könnte, wenn sich die zellulären Marker, die auf chronische inflammatorische Aktivität hinweisen, pharmakologisch günstig beeinflussen ließen.
Activation of the contact phase of coagulation has been implicated in the pathogenesis of septic shock. We wanted to determine if inhibition of plasma kallikrein can prevent arterial hypotension and liberation of kinins from kininogen, induced by an infusion of bacterial lipopolysaccharide (LPS) in anesthetized, ventilated 20-kg pigs. The LPS was given IV in a dose of 5 [mu]g/kg/h for 8 hours. The plasma kallikrein inhibitor aprotinin, 537 [mu]mol, was given IV during 8 hours, resulting in plasma levels above 10 [mu]mol/L. Ten animals (SA) received LPS and aprotinin and ten randomized controls (SC) received LPS and saline. Kinin-containing kininogen was determined on the basis of the amount of kinin releasable in plasma samples by incubation with trypsin. Kininogen decreased to 58% +/- 4% of the baseline value without any difference between groups. This may indicate participation of other processes than degradation by plasma kallikrein in the decrease of kininogen. Arterial blood pressure was higher at 7 hours in the SA animals than in the SC group (101% +/- 11% vs. 68% +/- 8%; mean +/- SEM; p = 0.026). Fibrin monomer and C3adesArg plasma levels were attenuated by aprotinin treatment. These findings underscore the important role of the contact system in LPS shock.
Mon, 1 Jan 1990 12:00:00 +0100 https://epub.ub.uni-muenchen.de/9788/1/9788.pdf Fritz, Hans; Jochum, Marianne; Geiger, R.; Weipert, J.; Spannagl, Michael; Siebeck, Matthias; Hoffmann, H.
Wed, 1 Jan 1986 12:00:00 +0100 https://epub.ub.uni-muenchen.de/6180/1/6180.pdf Lehnert, Peter; Giercksky, K.-E.; Burhol, P. G.; Revhaug, A.; Jenssen, T. G.; Riepl, Rudolf L.
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