Podcasts about dcx

This episode dives into one of the most important — and often underestimated — forces driving growth in the financial world today: Digital Customer Experience (DCX).For decades, financial institutions relied on product innovation, regulatory advantages, and balance sheet strength. But as those advantages level out and customer expectations rise, a new competitive edge is transforming the game: exceptional digital experiences.The episode unpacks ten powerful areas where digital experience design fuels business growth, from acquiring new customers and building unforgettable brands to creating emotional connections, “wow” moments, and intelligent cross-selling opportunities. You'll also discover how DCX enables scalable personalization, continuous improvement, digital trust, faster innovation cycles, and cost savings through smart self-service. Find out:Why DCX is redefining sustainable growth for banks, fintechs, and financial brandsWhich dimensions of digital experience most powerfully shape customer behavior, loyalty, and advocacyHow financial leaders can embed digital experience thinking into their culture, strategy, and innovation roadmapsIf you're curious about how digital can transform not just touchpoints but your entire financial brand, this episode is for you.Read the full article on UXDA's blog: https://theuxda.com/blog/digital-customer-experience-growth-driver-for-financial-institutions* AI podcast on UXDA article powered by Google NotebookLM

Ryan, Miles, Dan, and Pete are joined by fellow DcX member Matt Looper. We go over the most recent events and the January DcX Monthly Invitational

This solo episode of the DCX podcast is inspired by my recent job transition into a new role leading Customer Experience at Belfry Software. As I've just recently joined, I am actively engaged in the process of uncovering the opportunities which exist for automations to be implemented. The primary goals of implementing these automations are simple:1) Find places where automation can enhance the Customer Experience2) Find areas where automation can improve the Employee Experience Chapters:01:05 - New Sponsor: Thinkific Plus01:30 - Upcoming guests 02:45 - Digital CS Maturity Assessment03:29 - Alex got a new gig! 04:00 - Topic for today: Where to look for automations 06:43 - Support Data09:00 - Hours Sentiment Tracking10:28 - We automate for both the customer and employee experience11:53 - Frequently Distributed Documentation 14:10 - Onboarding16:36 - Upsell Automation 17:47 - Pre-Renewal Automation18:57 - Data Hygiene Automation22:11 - Customer Scorecards & Actions25:24 - Manual Process Automation27:59 - The big red flag no no: Don't automate without doing it manually first. Links: - Monthly Digital & Scale Meetup - digitalsuccess.gradual.us- Digital CS Maturity Assessment - https://digitalcustomersuccess.com/dcsmaturityThis episode of the DCX Podcast is brought to you by Thinkific Plus, a Customer Education platform designed to accelerate customer onboarding, streamline the customer experience and avoid employee burnout. For more information and to watch a demo, visit https://www.thinkific.com/plus/ Support the show+++++++++++++++++Like/Subscribe/Review:If you are getting value from the show, please follow/subscribe so that you don't miss an episode and consider leaving us a review. Website:For more information about the show or to get in touch, visit DigitalCustomerSuccess.com. Buy Alex a Cup of Coffee:This show runs exclusively on caffeine - and lots of it. If you like what we're, consider supporting our habit by buying us a cup of coffee: https://bmc.link/dcspThank you for all of your support!The Digital Customer Success Podcast is hosted by Alex Turkovic

Chris Meroff is a founder, author and serial entrepreneur with a passion for helping business professionals find fulfillment in their work through sustainable success. In 2011, he started his first company, MSB Consulting, and sold it in 2023 to fund his venture capital firm, Dirigo Capital Partners. Dirigo owns a variety of startups in various industries throughout Austin and along the coast of Maine, ranging from hospitality, farming and community development. One of these ventures is DCX Community which was born out of his passion to help develop leaders both personally, professionally, spirituality, and relationally, connect them to authentic community, and then experience transformation in their work, home, and cities. DCX does this by hosting an annual leadership conference and monthly networking event, The Table Network. He and his colleagues also host The Table Network Podcast, where they have intentional conversations with successful business owners and authors in order to build deeper relationships and better businesses. Chris blends his two worlds by hosting leadership retreats at his farm – West Branch Farms in Maine. He purchased the farm in 2020 to boost the economy by bringing jobs and resources to the local community. This heartbeat prompted him to open Coffee + Crisp Cafe and Foss Point Wedding Venue to provide locals various opportunities to engage.Chris is also a USA Today and Wall Street Journal bestselling author of his first book, Align: Four Simple Steps for Leaders to Create Employee Fulfillment. He wrote this book in 2019 to share with leaders the four simple steps that will transform the way you lead, renew your self-confidence and spark change. Align offers leaders to develop the courage to connect with their team in a meaningful way and start winning together. This led him to write his second book The Empathy Revolution: Practical Wisdom to Combat Organizational and Social Loneliness, where Chris shares his philosophy in a practical guide on why the current model of leadership isn't working. He addresses that in order to retain employee talent and engagement, employees must grasp the vision and align with the values laid out from the top down. With over 25 years of experience owning and operating businesses, Chris has dealt with a diverse group of clients to employees to competition. He grew up in the Northeast and has always been passionate about employing his community. Currently, he resides in Austin, Texas with his wife and has four adult children. Find out more:chrismeroff.comdcxcommunity.com

Today, we chat with Craig about this astonishing growth, covering topics ranging from their new e-commerce academy, to tips on integrating new DCX employees with your team, the incredible cultural exchange happening between American and Filipino employees, plus, the new DCX + commonsku collab!

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2023.06.26.546509v1?rss=1 Authors: Roshan, S. A., Gunaseelan, D., Swaminathan, J., Kandasamy, M., Muthuswamy, A. Abstract: Aim: After focal cerebral ischemia, intermittent hypoxia therapy (IHT) could be used as a non-invasive method to stabilize and stimulate neurogenesis in the innate stem cell niche in the brain, and disrupt the glial scar around the infarct to increase neuroblast migration in the striatal infarct area. Methods: We induced focal cerebral ischemia in Wistar albino rats using the MCAo model. A week later, animals were subjected to intermittent hypoxia (12%O2, 4hr/day) for a period of 14 days. Post-treatment analysis of functional recovery and cellular regeneration was done using immunofluorescence analysis of multiple neuronal cell markers including Doublecortin (DCX), Nestin, and Vimentin among others. Results: Observations of GFAP-positive cells revealed that IH treatment facilitates gliogenesis in the infarct striatal region of a rat model of MCAo stroke. The percentage of DCX and GFAP double-positive cells was increased in the IH-treated group. Also, there was a significant difference in the morphology of vimentin-positive cells and microglia cells between the stroke groups. Conclusion: These outcomes suggest that exposure of MCAo stroke-affected rats to intermittent hypoxia results in an increase in migrated neuroblasts resulting in a subsequent altered glial scar integrity in the infarct region, thus suggesting an alternative non-invasive method against the common stem cell transplant techniques, to increase endogenous neuroblasts in the infarct area after stroke. Copy rights belong to original authors. Visit the link for more info Podcast created by Paper Player, LLC

Matsyanyaaya: Insights from recent OEWG discussions on Information and Communications Technologies— Anushka SaxenaThe militarisation of cyberspace is a reality. And to enable states to discuss and adopt common rules for global governance of cyberspace, on 31 December 2020, the United Nations General Assembly adopted resolution 75/240  establishing an Open-ended Working Group (OEWG) on the security of and in the use of Information and Communications Technologies. The mandate for the Group extends from 2021 to 2025.The Group recently concluded its informal, inter-sessional meetings on 26 May, and deliberations put forth by various states give us some insights into the kind of talking points we could look out for during the fifth Substantive Session of the Group, scheduled for July 2023.To summarise, various stakeholders, ranging from governments and representatives of UN bodies to scholars from think tanks and technology corporations, submitted ideas about what the 2023 Annual Progress Report (APR) should entail. All of their ideas either build on or expand what has already been discussed in the previous substantive and informal sessions in 2023 or the 2022 APR. Some interesting ideas are as follows:* Iran submitted a Working Paper on establishing a provisional directory of 'Points of Contact' (PoCs) on ICT and cybersecurity.●     The first proposal to develop such a global directory was tabled in the UN Governmental Group of Experts Reports of 2013 (A/68/98). Now, every GGE and OEWG discussion notes progress on the directory.●     The aim of this directory shall be for states to appoint field experts in technical or diplomatic positions (or both), which would be a part of a global PoC network debating everything from responsible state behaviour in cyberspace and the applicability of international law to defining threats to ICT.●     As we know, the current Indian government has quite a knack for portals, and to formalise the creation of a PoCs global directory, India, too, has proposed the creation of a Global Cyber Security Cooperation Portal. The proposal, submitted by India's Permanent Representative in New York in July 2022, states that such a Portal shall be voluntarily updated by states and maintained by the UN Office for Disarmament Affairs.* The UNOCT/UNCCT and the UN Counter-Terrorism Committee Executive Directorate presented proposals for 'capacity building'. The proposal by the former was basically about glorifying the successes of its Global CounterTerrorism Programme on Cybersecurity and New Technologies. But the latter proposal, presented by the UNCTED, emphatically highlights the challenge of malicious online activity by rogue non-state actors and how existing counter-terrorism infrastructure can be leveraged to deal with it.●     The important recommendation is to develop comprehensive training programmes for law enforcement personnel and criminal justice practitioners working with digital evidence. The mention of the latter may be an important signal of more private sector participation in navigating the legalities of what constitutes 'terrorism' in cyberspace.* Submissions from the private sector mainly highlighted which governmental proposals are the most crucial for focus on in the next substantive session and how they can be expanded or narrowed down:●     Stimson Center's submission iterated that the two major emerging technologies states should agree on are common threats to ICT Security are ransomware and Artificial Intelligence.●     It should be noted that both El Salvador and Czechia had made statements during the last substantive session in March on the need for developing standards on 'responsible state behaviour' in new and emerging tech like AI and Quantum. But these efforts would be futile until states can first agree on what harmful use of AI/ Quantum is, given the dual nature of such technologies, and then move on to standard-setting.●     DCX Technologies presented anecdotes on how to avert a ransomware attack and engage with the attacker. Two suggestions stand out from their four-page intervention – one, that knowledge of critical infrastructure is essential to know how to protect it (such as by using enterprise security tools to detect malicious behaviour), and second, that any response to a large-scale ransomware attack such as the one DCX faced in 2020 requires a transparent, multi-stakeholder mitigation model.If adopted and developed, these ideas could provide meaningful direction for the next set of discussions at the OEWG-ICT. However, if we look at some of the concerns governments presented during the fourth substantive session of the Group earlier in March, we can safely conclude that some of these ideas are a massive jump ahead of the tide. For example, India's primary concern during the session was as fundamental as something can be – for states to converge on their definitions and interpretations of international law! Similarly, Kenya's proposal entailed that states at least converge on how to define 'common threats in the cyberspace'.This is, however, not to say that there exist no agreements whatsoever – states at the OEWG have now come to agree that the UN Charter is readily applicable to cybersecurity (especially provisions under Articles 2(1), 2(4), and 33). In doing so, they have cemented the idea that existing global governance institutions like the International Court of Justice can be utilised even to resolve cyber-incident disputes peacefully. This has not stopped countries like Russia and Syria from proposing a new legally-binding mechanism to govern state behaviour in ICT, citing the inability of existing mechanisms to do so. Overall, some convergence exists on building capacity, creating a global knowledge base involving both state and non-state actors, and creating a due diligence mechanism for states to respond to malicious activities originating from their territory. The next Substantive Session would be vital to understand how states respond to these ideas and whether they can agree to resolve some of the fundamental challenges facing the OEWG's ambitious goals.Cyberpolitik : A “broadly” unclear Light-Touch Regulation for India's Online Gaming Industry.— Satya SahuOnline gaming is one of the fastest-growing segments of India's digital economy, with millions of users playing various games on platforms ranging from smartphones, consoles and PCs. India's gaming population is pegged to reach 700 million by 2025, with a significant portion of players spending real money on games. (current conversion rate is about 24% or 120 million players. It is a good bet that this trend will comfortably allow the Indian online gaming industry's ambitions of growing to USD 8.6 billion by 2027. However, online gaming also comes with challenges and risks, because it can serve as a pathway to gambling using real money, addiction, an easy target for cybercrime, and exposure to illegal illicit content.So of course, the Ministry of Electronics and IT (MeitY) notified amendments to the Information Technology (Intermediary Guidelines and Digital Media Ethics Code) Rules, 2021, related to online gaming in early April 2023.  The amendments aim to enforce greater due diligence by online gaming intermediaries, such as platforms, websites, and apps that offer online games, and to protect users from illegal betting and wagering online. The amendments also envisage the creation of self-regulatory bodies (SROs) that will register and certify permissible online games and resolve complaints through a grievance redressal mechanism.In most regards, the amendments have garnered applause from the gaming industry for being an unusual example of a light-touch regulation and promoting the idea of a trustworthy self-regulating market. It is a rare example of an enabling legislation meant to promote regulatory certainty without much in the way of prescriptive mandates. But with the lack of prescription, also comes uncertainty, particularly in the matter of definitions involved in deciding what constitutes "online gaming", "betting”, or "gambling. While jurisprudence across the country is settled on the distinction being whether the game in question has an element of skill or an element of chance (with the latter legally prohibited), the Rules do not provide any assistance in making that distinction clear.There is also a significant issue about the implementation of these regulations due to the fact that gambling is a state list subject under the Indian constitution; however, the discussion on federalism in this context is beyond the scope of this post.This post's focus, however, is the definition of “user harm” in the context of online gaming. As per the explanation to Rule 3(1)(b)(ii), “user harm” and “harm” mean any effect which is detrimental to a user or child, as the case may be.Even at a cursory glance, this definition is unusually broad and vague, leaving much room for interpretation and discretion by the government and the SROs. For instance, would considerations of obscenity, defamation, hate speech, discrimination, harassment, cyberbullying, cyberstalking, phishing, hacking, identity theft, addiction, or compulsive behaviour etc be relevant while defining “harm” in the context of online gaming? How will these terms be defined and measured? Who decides whether an online game is likely to incite any of these harms? What are the criteria and standards for such decisions? How will the users be informed and educated about these harms and their consequences?Moreover, a definition of user harm that does not take into account the diversity and complexity of online gaming genres, formats, modes, and audiences would be woefully limited. Online gaming is not a monolithic phenomenon, but a rapidly evolving one, with different types of games catering to different players.In games, the depiction of drug use, violence, and sexually explicit content is handled by certification and age-rating systems like ESRB and PEGI in the US and the UK respectively, with generally consistent decision-making. In the case of India, the Rules mention the objective of tackling content-related concerns in terms of depiction of violent or inappropriate content. However, Rules 4A(8) and 4C have imposed an obligation on the SRO to ensure that the verification process to determine a game's permissibility be based on a self-devised framework which assesses whether an online game contains adequate safeguards against user harm. The only considerations to be used while formulating said framework, are “self harm and psychological harm”, which do not do much to circumscribe our definitional woes. The idea of the SROs to also act as a classification and age-rating body is a possible step in the right direction assuming that multiple SROs will not create conflicting frameworks for verification. While India's approach may end up as a beefed-up version of the US and the UK (with legal liabilities on the online gaming intermediaries, and direct oversight of the Union Government etc.) , the case of Australia's National Classification Code should serve as a warning of the kind of distortions that can be created in a regulatory regime when overbroad concepts are used to define what constitutes “harm” to the player. Australia's Office of Film and Literature Classification, bound by their legislative regime, can reject certification for a game if its depiction of sex and drug use is potentially portrayed “positively”. Because age-ratings and classifications directly impact the commercial success of games (as well as movies, which is usually used as a counterpoint against controversial classification systems which do not keep up with the changing nature of multimedia consumption), the Indian gaming market can potentially find themselves reworking key aspects of their games just to be able to get them onto the market. It is a costly endeavour to say the least.As all these teething questions abound, one only hopes that a consistent framework is proposed to guide interpretations regarding the ambit of "user harm" before dispute redressal and adjudication processes inevitably commence in the future.Antariksh Matters : China's in a Hurry to Get to the Moon— Aditya RamanathanChina has announced an official deadline of 2030 for landing humans on the lunar surface. On Monday, Lin Xiqiang, the deputy head of the China Manned Space Agency (CMSA) said the mission to put humans on the Moon was underway and would include a programme of research during short visits. Lin's announcement confirms a public comment in April by Wu Weiren, a scientist with China's lunar exploration programme, who said putting humans on the Moon by 2030 was “not a problem”. China has been steadily developing its crewed lunar programme. In 2022, it unveiled a model of a 90-metre-long moon rocket scheduled to undergo a flight test in 2027. Earlier in 2019, a promotional video showed off what appeared to be a crewed vehicle for deep space travel being developed by the China Academy of Space Technology (CAST). China's ongoing pursuit of sustained human presence in low Earth orbit will contribute to its ability to send people to the Moon. Lin's official confirmation came at a press conference in which he also presented the new three-person crew for the Tiangong space station, which will launch into orbit this week, replacing three others who have been inhabiting the space station for six months. The experience with Tiangong will especially come in handy if China manages to proceed to the next stage of its lunar project: setting up a permanent base on the Moon. Lunar LivingIn 2021, China and Russia entered into an agreement to establish a permanent presence on the Moon. Eventually dubbed International Lunar Research Station (ILRS), the project was meant to be a direct counterpart to the United States' Artemis programme, which, as of this writing, still intends to return humans to the Moon by 2025 and eventually set up a permanent presence on the lunar surface and in orbit.In April, Wu publicly discussed a multi-stage plan for the ILRS up to 2050. This would include uncrewed missions and the setting up of a “basic version” that will be followed by a “full version” put together by 2040. Other stages include setting up a nuclear power source and research infrastructure. As with Artemis, China plans to support all this by putting a large constellation of satellites into lunar orbit for position navigation and timing (PNT), relay communications to the dark side of the Moon, and remote sensing. Earthly ConstraintsILRS may have begun as a Russia-China collaboration, but since the outbreak of the war with Ukraine, Russia has been conspicuous by its absence from recent Chinese statements. Instead, China has focused on its own plans and has sought other foreign partners for its upcoming Chang'e uncrewed missions to the Moon. China's lunar ambitions are also evidently fuelled by its rivalry with the United States. However, China does not have the option of blending competition with a bit of cooperation. In 2011, the US introduced the so-called ‘Wolf Amendment', which effectively bans US government funding to be used in cooperation with any Chinese entity without clearance from the Federal Bureau of Investigation (FBI). While this is not technically an outright ban on space collaboration with China, its effect is much the same. Indeed, it seems clear that NASA is determined to keep away from China. NASA's administrator Bill Nelson has made alarmist remarks about China appropriating lunar territory, presumably to bolster support for the Artemis programme. However, if China and the US are engaged in a space race to the Moon, it is a relatively muted affair at the moment. Top politicians have not expended political capital on the issue, and space agencies have not seen an explosion in their budgets. The lunar ambitions of great powers will continue to be subject to Earthly constraints like economic downturns, wars, stubborn technological challenges, and myriad other pressing issues. Our Reading Menu[Podcast] - A Day in the Life of a Cop, a new limited series on 'policing' on All Things Policy, by Shrikrishna Upadhyay and Javeed Ahmed.[Op-ed] Rs 2,000 Note Withdrawal: No demonetisation redux but RBI could have done it better, by Anupam Manur.[Report] Defense Primer: U.S. Policy on Lethal Autonomous Weapon Systems, by Kelly M. Sayler. This is a public episode. If you would like to discuss this with other subscribers or get access to bonus episodes, visit hightechir.substack.com

In this episode of the Real Estate Podcast, we sit down with Chris Meroff, a successful entrepreneur and owner of several businesses, including an event collective and a venture capital and private equity firm. Chris shares his journey into entrepreneurship, including the challenges he faced along the way and the importance of being willing to trade power for the greatness of others. We dive into the topic of vulnerability and its role in leadership, as Chris opens up about his struggles with emotional loneliness and how he overcame it by implementing vulnerability in his leadership style. He emphasizes the importance of empathy and community in achieving our goals and becoming the people we want to be. Chris also discusses his current ventures, including Wilco Workspaces and ROI Physical Therapy and Sports Performance, as well as his investment strategy in real estate. We also learn about his DCX community, which focuses on personal development, connection, and transformation. Join us as we explore the power of vulnerability and empathy in leadership with Chris Meroff, and discover how these qualities can lead to greater success in both personal and professional life. Learn more about joining The enTREpreneur™ Community here

Comment parler de son vécu, de ses traumas et de son intimité tout en se préservant et sans être tokenisé·e ? Dans ce deuxième épisode, de PRÉSENT.E en partenariat avec le Centre d'art contemporain de La Ferme du Buisson, je reçois deux des artistes de l'exposition : Nesrine Salem et Théophylle Dcx ! Un épisode enregistré dans le cadre de l'exposition Les Sillons, présentée au Centre d'art contemporain de la Ferme du Buisson jusqu'au 16 juillet prochain . . Références citées dans l'épisode : L'artiste Lavande Chaïma Boumaaz Artagon Marseille La Pensée Straight de Monique Wittig . Épisode retranscrit : https://podcastpresente.fr/2023/04/27/les-sillons-2-4/ . Crédits : Présent.e est un podcast produit, réalisé et diffusé par Camille Bardin. Cet entretien a été enregistré en mars 2023 à La Ferme du Buisson dans le cadre d'un partenariat avec le centre d'art. Réalisation et mixage : Camille Bardin.

Sourceshttps://space.nss.org/the-spaceship-that-came-in-from-the-cold-war-the-untold-story-of-the-dc-x/https://en.wikipedia.org/wiki/McDonnell_Douglas_DC-X https://www.airandspaceforces.com/article/0261propulsion/ https://www.amusingplanet.com/2021/03/dc-x-rocket-that-beat-spacex-by-20-years.html https://www.semanticscholar.org/paper/History-of-the-Phoenix-VTOL-SSTO-and-recent-in-Hudson/7cf9c424f6e04c620b1ae69283ffbfd984dbd6d0 https://en.wikipedia.org/wiki/Gary_Hudson_(engineer)#/media/File:Gary-Hudson-at-Freeland-Conf.jpg https://en.wikipedia.org/wiki/Cash%E2%80%93Landrum_incident Please Support Strange Recon!Paypal - https://www.paypal.com/paypalme/strangereconVenmo - https://venmo.com/u/strangereconpodcast Google - strangerecon@gmail.com Donate via the website! - https://www.strangereconpodcast.com/Listen to the Show!Apple https://podcasts.apple.com/us/podcast/strange-recon-radio/id1545754747Spotify https://open.spotify.com/show/5qqid6saqCqIUGCkPXGKp4Spreaker https://www.spreaker.com/show/strange-recon-podcastGoogle https://podcasts.google.com/feed/aHR0cHM6Ly93d3cuc3ByZWFrZXIuY29tL3Nob3cvNDYyMDgzMC9lcGlzb2Rlcy9mZWVk?sa=X&ved=2ahUKEwj09Z_Ix43-AhUPH2IAHXdTDt0Q9sEGegQIARACIheartRadio https://www.iheart.com/podcast/966-ep-1-galactic-federation-p-75476164/ Audible https://www.audible.com/pd/Strange-Recon-Podcast-Podcast/B08K563YJS?qid=1680519726&sr=1-1&ref=a_search_c3_lProduct_1_1&pf_rd_p=83218cca-c308-412f-bfcf-90198b687a2f&pf_rd_r=EHPZW5760ESNNNJ2M8E0&pageLoadId=yaTbQ4OGn9Hc3Z8h&creativeId=0d6f6720-f41c-457e-a42b-8c8dceb62f2c

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2023.02.08.527694v1?rss=1 Authors: Prina, M., Esteve-Perez, R., Navarro-Moreno, C., Abellan-Alvaro, M., Lanuza, E., Sanchez-Catalan, M. J., Martinez-Garcia, F., Agustin-Pavon, C., Torres-Perez, J. V. Abstract: In mammalian species, motherhood is a critical period of neural plasticity, allowing the dam to adapt its behaviour to the demanding task of taking care of the pups. For example, in mice, pregnancy and lactation promote proliferation of neuroblasts in the subventricular zone and adult neurogenesis in the olfactory bulb. Thus, we hypothesised that the non-canonical neurogenesis described in other areas of the olfactory system would be similarly affected by either pregnancy or lactation. To test this hypothesis, we analysed the expression of doublecortin (DCX) and Ki67, markers of immature neurons and proliferation respectively, across the reproductive cycle in adult CD1 female mice. In a group of late pregnant females, there was a trend towards increase of DCX cells in the olfactory bulb. In the piriform cortex, lactating females had significantly fewer immature neurons, and of lower complexity and diameter, than pup-sensitised females. This suggests that both lactation, and/or exposure to pups, and the preceding pregnancy are require to promote maturation of this population of embryonically generated DCX-ir neurons. By contrast, the number of DCX-immunoreactive cells in the olfactory tubercle was not significantly affected by motherhood. Finally, we found Ki67 nuclei located in close apposition to clusters of DCX-ir cells in this region, with some of DCX-ir neurons co-expressing both markers. Taken together, our results expand our current knowledge on how motherhood remodels the brain and reveal the olfactory tubercle as an unexplored niche for adult neurogenesis. Copy rights belong to original authors. Visit the link for more info Podcast created by Paper Player, LLC

The second of three episodes recorded in partnership with Freemarket at FinTech Connect, that took place at the Excel in London from 30th November – 1st December. We recorded a series of interviews from the Freemarket booth with a number of the speakers and attendees at the event. Our guests for this second episode were: 1/ Susanne Chishti, CEO, FinTech Circle 2/ Matt Jackson, VP, Relationship Management, Freemarket 3/ Matt Bonetti, Head of DCX, Hargreaves Lansdown 4/ Robin Scher, Head of Strategic Fintech Investing, Lloyds Banking Group 5/ Hirander Misra, Chairman & CEO, GMEX Group 6/ Janusz Diemko, Co-Founder, Xelo Pay 7/ Susana Ponce-Froment, Head of Financial and Credit Risk, Tide

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2022.09.30.510371v1?rss=1 Authors: Yagi, S., Wen, Y., Galea, L. Abstract: Estrone and estradiol differentially modulate neuroplasticity and cognition but how they influence maturation pathways of new neurons is not known. The present study assessed the effects of estrone and estradiol on various aspects of neurogenesis in the dentate gyrus (DG) of ovariectomized young adult Sprague-Dawley rats using daily subcutaneous injections of 17{beta}-estradiol or estrone. Rats were injected with a DNA synthesis marker, 5-bromo-2-deoxyuridine (BrdU), and were perfused one, two, or three weeks after BrdU injection and treatment. Immunofluorescent labelling for Sox2 and Ki67 were used to examine the density of neural stem cells and proliferating cells, respectively. Double-immunofluorescent labelling of BrdU with doublecortin (DCX) or NeuN was used to examine the attrition and maturation of adult-born neurons over time. Estradiol reduced the density of neural stem cells in the dorsal DG, whereas estrone reduced the density of neural stem cells in the ventral DG. Furthermore, estradiol enhanced, whereas estrone reduced, cell proliferation after one week but not after longer exposure to hormones. Both estrogens increased the density of BrdU/DCX-ir cells after one week of exposure but showed greater attrition of new neurons between one and two weeks after exposure. Lastly, estradiol decreased the percentage of BrdU/NeuN-ir cells in the dorsal DG after three weeks of treatment. These results demonstrate that estrogens have differential effects to modulate several aspects of adult hippocampal neurogenesis in the short term, but fewer effects after long-term exposure and that estradiol and estrone modulate neurogenesis via different pathways. Copy rights belong to original authors. Visit the link for more info Podcast created by PaperPlayer

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2022.09.23.509244v1?rss=1 Authors: Alderman, P. J., Saxon, D., Torrijos-Saiz, L. I., Sharief, M., Biagiotti, S. W., Page, C. E., Melamed, A., Kuo, C. T., Garcia-Verdugo, J.-M., Herranz-Perez, V., Corbin, J. G., Sorrells, S. F. Abstract: The human amygdala paralaminar nucleus (PL) contains immature excitatory neurons that exhibit protracted maturation into adolescence; however, whether a similar population exists in mice is unknown. We discovered a previously undescribed region with immature doublecortin (Dcx)+ excitatory neurons adjacent to the mouse basolateral amygdala, and similar to humans, these neurons mature during adolescence and are distinct from adjacent intercalated cells. Despite their immature features, these neurons are born during embryogenesis, populate the mouse PL prior to birth, and remain in an immature stage of development until adolescence. In the postnatal brain, a subpopulation of these excitatory neurons surprisingly migrate into the neighboring endopiriform cortex, peaking between P21-P28. In humans, cells with the molecular identity of mouse PL neurons populate the PL as early as 18 gestational weeks, and also exhibit migratory morphology into adolescence (13 years). The finding of a similar region in both mice and humans suggests a potentially conserved cellular mechanism for neuron recruitment and migration during adolescence, a key time period for amygdala circuit maturation and behavioral changes. Copy rights belong to original authors. Visit the link for more info Podcast created by PaperPlayer

In this shorter episode we revisit some of the stories from the first ten shows. Today we go behind the scenes and hear the stories of how some world famous ads came to be, from Guiness Dancing Man and Quarrell to Paddy Powers Rhodri Giggs ad and the amazing Hovis Go On Lad. You will hear from * Marketing legend and author Paul Feldwick talk about the Rowan Atkinson Barclaycard ad "Rug" https://www.youtube.com/watch?v=g-oCckHaGdM * Former Paddy Power Head of Brand Emer McCarthy talks about Rhodri Giggs loyalty https://www.youtube.com/watch?v=cBlkxsbtlgw and also Jose Mourinho Jackpots https://www.youtube.com/watch?v=TerX8S-NHFk * Jon Goldstone, Managing Partner of the BrandGym gives the lowdown on the briliiant Hovis Go On Lad https://www.youtube.com/watch?v=s_suyZb5mDk * Kay McCarthy of MCCP shares something surprising about Guinness Dancing Man https://www.youtube.com/watch?v=5Om15TM7t9g * Damian Devaney of TBVGlobal chats about working with Michael Fassbender in Guinness Quarrel and what they got up to on set https://www.youtube.com/watch?v=mSh1NNp0q5Q * Doug Cameron, founding partner of DCX shares how the remarkable Payless Shoes - Payless Experiment "Palessi" campaign was born https://www.youtube.com/watch?v=-2gaNq-rOkI* Paul Dervan CMO with the National Lottery talks about one of his favourite campaigns "O2 Play Them Next" https://www.youtube.com/watch?v=wl1vwr6L1R4 Hosted on Acast. See acast.com/privacy for more information.

In this week's episode, Conor Byrne chats to Doug Cameron, Founder and CEO of DCX. Doug, from his home in Brooklyn New York, reveals how he met advertising tycoon, David Ogilvy, and how you can begin to consider a cultural strategy, by cultivating a more genuine relationship with not only the people who need your product/service, but potential employees and collaborators too. Hosted on Acast. See acast.com/privacy for more information.

Today's episode is all about mental health, depression, anxiety, and the effect that these things can have on the way that we run our businesses. Benny Lewis is the founder of Fluent in 3 months, and he recently opened up about his own struggles at our unofficial DCx event in London a few weeks ago. While his business was "successful" by many traditional metrics, his personal life was far from it. He soon found himself under a mountain of credit card debt, with his marriage to his partner of five years ending in divorce. Benny joins us on this week's podcast to share the details of his personal journey. You'll hear about his battle with clinical depression, the steps he took to get back on his feet, and how his business was able to survive through such a tumultuous period in his life.

暗号資産（仮想通貨）・ブロックチェーンに特化した幻冬舎のメディア「あたらしい経済 https://www.neweconomy.jp/ 」がおくる、ラジオ番組です。毎日最新ニュース解説とコラムをお届けします。 ・UUUMもNFT参入、グループ企業FOROが今夏マーケットプレイス開始 ・西野亮廣『みにくいマルコ』NFTがOpenSeaでオークション開始、フィナンシェと協業 ・インスタグラム、NBA Top ShotのDapper LabsおよびNBAと提携か ・アシックスがNFT販売、落札者にはNFTシューズのアクセス権が付与 ・国内初、GMOコインがコスモス（ATOM）を取扱い開始 ・ディーカレット、国内初のオントロジー（ONT）上場へ ・南米初、ブラジルでイーサリアムETFが承認 ・グレイスケールが大手銀行BNYメロンと提携、ビットコインETFの準備開始 ・S&Pダウ、240以上の暗号資産を含んだ新たな評価指数を発表 ・ロンドン警視庁、275億円分の暗号資産押収 ・分散型取引所クリッパー、ポリチェーンらから2,100万ドル調達 ・オーストラリア政府、ブロックチェーン関連の2プロジェクトに約4.6億円資金提供 ・富士通がシンガポールのDCXとの提携と出資を発表、コモディティ取引のグローバル展開が狙い ・コインベースウォレットがポリゴンに対応 ・ビットポイント、イーサリアムのレバレッジ取引開始 ・コパアメリカ公式NFT、決勝のデジタル記念品としてリリース ・韓国サムスン、ブロックチェーン活用の新サービス「ペーパーレス」開始 ・ステイクテクノロジーズ、Web3財団より7度目の助成金獲得 ・NFTのAMLソリューション提供目指し、ハッシュポートと英エリプティックが提携 ニュースの詳細や、アーカイブやその他の記事はこちらから https://www.neweconomy.jp/

Here are the top cryptocurrency news headlines from India this week:Indian crypto investors weigh options after multiple reports of a blanket crypto ban: https://www.bloombergquint.com/business/indias-crypto-investors-weigh-options-ahead-of-impending-ban ;Tesla’s Bitcoin investment sparks hope in India: https://www.youtube.com/watch?v=IiGDL8ysr9I&t=1sCoinDCX announces global crypto-to-crypto trading platform Cosmex: https://www.cryptodost.io/exchange/coindcx-announces-cosmex-new-crypto-to-crypto-trading-platform-for-global-audience.html ;High Court dismisses FIR filed against Unocoin co-founders: https://news.unocoin.com/?p=1104 ;CoinDCX lists EasyFi’s EASY token on DCX Margin;WazirX announces support for the QiSwap airdrop for QTUM holders

In this first episode of CX Decoded, we explore the challenges and priorities of CX professionals as we delve into our annual research report, the State of DCX. Hosts Rich Hein, head of content for CMSWire, and his colleague, Sarah Kimmel, VP of research at Simpler Media Group, do a deep dive on CMSWire's State of the Digital Experience survey research during this CX Decoded podcast by CMSWire. They are joined here by special guest Annette Franz, CCXP, founder and CEO of CX Journey, Inc. The podcast was recorded in December of 2020. Report: State of the Digital Customer Experience

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2020.11.03.366468v1?rss=1 Authors: Spirhanzlova, P., Sebillot, A., Vancamp, P., Gothie, J.-D., Le Mevel, S., Leemans, M., Wejaphikul, K., Meima, M., Mughal, B. B., Butruille, L., Roques, P., Remaud, S., Fini, J.-B., Demeneix, B. A. Abstract: North-Eastern Brazil saw intensive application of the insecticide pyriproxyfen (PPF) during the microcephaly outbreak caused by Zika virus (ZIKV). ZIKV requires the neural RNA-binding protein Musashi-1 to replicate. TH represses MSI1. Being a suspected TH disruptor, we hypothesized that co-exposure to the main metabolite of PPF, 4'-OH-PPF, would exacerbate ZIKV effects through increased MSI1 expression. This was tested using in vitro mouse neurospheres and an in vivo TH signaling reporter model, Xenopus laevis. TH signaling was decreased by 4'-OH-PPF in both models. In mouse-derived neurospheres the metabolite reduced neuroprogenitor proliferation as well as markers of neuronal differentiation. The results demonstrated that 4'-OH-PPF significantly induced MSI1 at both the mRNA and protein level, as well as Fasn mRNA. Other TH target genes were also significantly modified. Importantly, several key genes implicated in neuroprogenitor fate and commitment were not dysregulated by 4'-OH-PPF alone, but were in combination with ZIKV infection. These included the neuroprogenitor markers Nestin, Egfr, Gfap, Dlx2 and Dcx. Unexpectedly, 4'-OH-PPF decreased ZIKV replication, although only at the fourth and last day of incubation, and RNA copy numbers stayed within the same order of magnitude. However, intracellular RNA content of neuroprogenitors was significantly decreased in the combined presence of the PPF metabolite and ZIKV. We conclude that 4'-OH-PPF interferes with TH action in vivo and in vitro, inhibiting neuroprogenitor proliferation. In the presence of ZIKV, TH signaling pathways crucial for cortical development are significantly impacted. This provides another example of viral effects that are exacerbated by drug or pesticide use. Copy rights belong to original authors. Visit the link for more info

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2020.10.02.324285v1?rss=1 Authors: Song, J., Merrill, R. A., Usachev, A. Y., Strack, S. Abstract: Proper brain development and function requires finely controlled mechanisms for protein turnover and disruption of genes involved in proteostasis is a common cause of neurodevelopmental disorders. Kelch-like 15 (KLHL15) is a substrate adaptor for cullin3 (Cul3)-containing E3 ubiquitin ligases and KLHL15 gene mutations were recently described as a cause of severe X-linked intellectual disability. Here, we used a bioinformatics approach to identify a family of neuronal microtubule-associated proteins (MAPs) as KLHL15 substrates, which are themselves critical for early brain development. We biochemically validated doublecortin (DCX), also an X-linked disease gene, and doublecortin-like kinases 1 and 2 (DCLK1/2) as bona fide KLHL15 interactors and mapped KLHL15 interaction regions to their tandem DCX domains. Shared with two previously identified KLHL15 substrates, a FRY tripeptide at the C-terminal edge of the second DCX domain is necessary for KLHL15-mediated ubiquitination of DCX and DCLK1/2 and subsequent proteasomal degradation. Conversely, silencing endogenous KLHL15 markedly stabilizes these DCX domain-containing proteins and prolongs their half-life. Functionally, overexpression of KLHL15 in the presence of wild-type DCX reduces dendritic complexity of cultured hippocampal neurons, whereas neurons expressing FRY-mutant DCX are resistant to KLHL15. Collectively, our findings highlight the critical importance of the E3 ubiquitin ligase adaptor KLHL15 in proteostasis of neuronal MAPs and identify a regulatory network important for development of the mammalian nervous system. Copy rights belong to original authors. Visit the link for more info

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2020.06.08.140467v1?rss=1 Authors: Moura, D. M. S., Brandao, J. A., Lentini, C., Heinrich, C., Queiroz, C. M., Costa, M. R. Abstract: Cell lineage in the adult hippocampus comprises multipotent and neuron-committed progenitors. In the present work, we fate-mapped neuronal progenitors using Dcx-CreERT2 and CAG-CAT-EGFP double-transgenic mice (cDCX/EGFP). We show that three days after tamoxifen-mediated recombination in cDCX/EGFP adult mice, GFP+ cells in the dentate gyrus co-expresses DCX and about 6% of these cells are proliferative neuronal progenitors. After 30 days, 20% of GFP+ generated from these progenitors differentiate into GFAP+ astrocytes. Administration of the chemoconvulsants kainic acid (KA) or pilocarpine (PL) led to a significant increase in the number of GFP+ cells in both ipsi and contralateral dentate gyrus. However, while PL favored the differentiation of neurons in both ipsi- and contralateral sides, KA stimulated neurogenesis only in the contralateral side. In the ipsilateral side, KA injection led to an unexpected increase of astrogliogenesis in the Dcx-lineage. These different effects of KA and PL in the Dcx-lineage are associated with distinct alterations of the parvalbuminergic plexus and inflammatory responses in the hippocampi. Finally, we also observed a small number of GFP+/GFAP+ cells displaying radial-glia morphology ipsilaterally 3 days after KA administration, indicating that Dcx-progenitors could regress to a multipotent stage. Altogether, our data suggest that cell lineage in the adult hippocampus is not unidirectional and can be modulated by environmental signals. Copy rights belong to original authors. Visit the link for more info

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2020.06.04.135350v1?rss=1 Authors: Cardoso, G. T., Gomes-Leal, W., Franco, E. C., Gama, J. S., Gomes, F. L., Brino, A. L. F., Lima, S. M. A. Abstract: Temporal lobe epilepsy (TLE) is the commonest type of focal epilepsy in adult humans. In refractory TLE, patients are indicated for unilateral resection of the affected hippocampus (hippocampectomy), which generally does not cause any cognitive impairment. Once adult hippocampus is a region of endogenous neurogenesis, we have hypothesized that a compensatory increase in hippocampal neurogenesis might occur in the remaining hippocampus after unilateral hippocampectomy. To test this hypothesis, we performed unilateral hippocampectomy in adult Wistar rats (n=12). Sham animals were not hippocampectomized (n=6). Animals were deeply anesthetized and adjacent cortex and hippocampus of the left hemisphere were completely removed. They were perfused at 15 (G15, n=6) or 30 (G30, n=6) days post-surgery. Behavioral tests were performed to address possible cognitive impairments. We did not find any cognitive impairment in the hippocampectomized animals. Histopathology was performed using thionine staining and mature neurons and migratory neuroblasts were immunolabeled using anti-NeuN and anti-doublecortin (DCX) antibodies, respectively. The remaining hippocampus presented higher numbers of DCX positive cells compared to control (p

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2020.05.26.115873v1?rss=1 Authors: Mendez-David, I., DAVID, D. J., Delomenie, C., Beaulieu, M., Gardier, A. M., Hen, R. Abstract: We investigated the mechanisms underlying the effects of the antidepressant fluoxetine on behavior and adult hippocampal neurogenesis (AHN). After confirming our earlier report that the signaling molecule {beta}2-arrestin is required for the antidepressant-like effects of fluoxetine, we found that the effects of fluoxetine on proliferation of neural progenitors and on survival of adult-born granule cells are absent in the {beta}2-arrestin knockout ({beta}2-Arr KO) mice. To our surprise fluoxetine induced a dramatic upregulation of doublecortin (DCX) in the {beta}2-Arr KO mice, indicating that DCX expression can be increased even though AHN is not. We discovered two other conditions where DCX expression is regulated non linearly compared to levels of AHN: a chronic stress model where DCX is upregulated and an inflammation model where DCX is down regulated. We conclude that assessing DCX expression alone to quantify levels of AHN can be misleading and that caution should be applied when label retention techniques are not available. Copy rights belong to original authors. Visit the link for more info

When you do business digitally, the customer is unmistakably the boss. After all, they can simply click away, unsubscribe, or otherwise dismiss your brand in a matter of seconds. Chances are your company is not the only one that offers what you offer. Your customers know that, and they want to buy from the company that treats them the best and gives them what they want. So, to put it bluntly, you had better get on the ball when it comes to your digital customer experience (DCX), or your business simply won't last. In a previous article, we wrote about the role of technology in the future of customer experience, and a big part of that had to do with analytics. Now, we want to expand on that idea and really dig into how analytics impacts DCX. Why? By far, the best way to improve your digital customer experience is to utilize your analytics to make the right improvements instead of guessing at what your customers want.

Pat Mayo, Geoff Fienberg and Tim Anderson discuss the latest 2020 NFL News, trade rumors, free agent destinations before recapping their 2019 NFL Win Total picks and making some early 2020 NFL Predictions, Also, Diet Coke Xtra was drank.  Vote in the 2020 Custy Awards: http://bit.ly/VoteCustys2020 AFC Win Total Recap: http://bit.ly/20QBRumVid NFL DraftKings Tips & Strategy: http://bit.ly/19DKVid SHOW INDEX 0:33 Giveaways 8:31 DCX  12:42 Brees/Bridgewater 19:44 Slay/Harris 25:42 Lev Bell 29:58 Matthew Stafford 36:53 Antonio Brown 39:56 Leonard Fournette 41:55 NFC Win Totals Recap 42:36 NFC West 50:43 NFC North 1:08:43 NFC East 1:07:20 NFC South Pat Mayo Twitter: https://twitter.com/ThePME Pat Mayo Facebook: https://www.facebook.com/ThePME  Pat Mayo Instagram: https://www.instagram.com/thepme/ All Pat Mayo Experience Versions:  Video: https://goo.gl/7rndwo Apple: http://bit.ly/PMEiTunes  Spotify: https://goo.gl/VboemH Stitcher: http://goo.gl/Ft3Gmr  Google: http://bit.ly/GooglePodPME Castbox: http://bit.ly/PMECastbox TuneIn: http://bit.ly/PMEtuneIn Podbean: https://goo.gl/eoDvt1 Radio Public: https://goo.gl/81b1ag iHeart Radio: https://goo.gl/UOmFXA Audioboom: https://goo.gl/4MpT6B 

Dans cet épisode de Startup Story spécial Gaming, nous nous focalisons sur le développement de jeux vidéo avec nos deux invités: Mohamed Ali Midani Directeur Exécutif de DCX et Brahim Jomni Cofondateur de Instead, qui nous ont parlé de leurs projets, leurs modèles économiques et bien plus. Producteur : Mohamed Nabli (Spoot) Producteur exécutif : Walid Naffati Enregistrement dans les studios de Jawhara FM Production : StreamingHD

The conversation continues and keeps getting better with Frank Wammes, CTO Continental Europe (https://twitter.com/fwammes)and Johan Hallberg, CTO DCX, Sweden (https://twitter.com/johanhallberg) Listen to this sensational podcast and send in your topic requests to Frank & Johan to address upon in their next podcast. Image Source: novoed.com

In this interview, I had the opportunity to interview Peter Lawson Jones, a prominent Cleveland-Shaker Heights figure as a lawyer, public official, and actor.  I met Mr. Jones last week at our annual ORSA Conference at Cleveland State University after his keynote address. To listen to the show, click on one of the following: Itunes Sticher Your computer Like on Facebook Mr. Jones shares riveting stories about his parents, his professional journey, being a proud husband and father, and the responsibility of citizens to be involved in the political process.  Here are some of the highlights: his parents being involved in significant civil rights activities, such as Tuskegee Airmen, the Negro Baseball League, and pioneers in several other regards appreciating the sacrifices others have made and building on that the concept of read with expression, which has been a foundation for many things in his life Values of service, integrity, hard work, selflessness, and education how his experience at Harvard and Harvard Law continues to serve him to this day managing his energy levels and time encouraging involvement in the political process, starting with educated voting, a fundamental tenet of our system important legislation he helped craft that he is proud of, such as the Ohio Commission on Fatherhood Matthew 25:36 passage Mr. Jones can be reached at peterlawsonjones@gmail.com or peterlawsonjones.com Go here to read my blog Siliko's Splendid Shorts

We have previously shown that transplantation of immature DCX+/NeuN+/Prox1+ neurons (found in the neonatal DG), but not undifferentiated neuronal progenitor cells (NPCs) from ventral subventricular zone (SVZ), results in neuronal maturation in vivo within the dentate niche. Here we investigated whether we could enhance the integration of SVZ NPCs by forced expression of the proneural gene Neurogenin 2 (NEUROG2). NPCs cultured from neonatal GFP-transgenic rat SVZ for 7 days in a non-differentiating medium were transduced with a retrovirus encoding NEUROG2 and DsRed or the DsRed reporter gene alone (control). By 3 days post-transduction, the NEUROG2-transduced cells maintained in culture contained mostly immature neurons (91% DCX+; 76% NeuN+), whereas the control virus-transduced cells remained largely undifferentiated (30% DCX+;

Background: Lack of appropriate tools and techniques to study fate and functional integration of newly generated neurons has so far hindered understanding of neurogenesis' relevance under physiological and pathological conditions. Current analyses are either dependent on mitotic labeling, for example BrdU-incorporation or retroviral infection, or on the detection of transient immature neuronal markers. Here, we report a transgenic mouse model (DCX CreERT2) for time-resolved fate analysis of newly generated neurons. This model is based on the expression of a tamoxifen-inducible Cre recombinase under the control of a doublecortin (DCX) promoter, which is specific for immature neuronal cells in the CNS. Results: In the DCX-CreERT2 transgenic mice, expression of CreERT2 was restricted to DCX+ cells. In the CNS of transgenic embryos and adult DCX-CreERT2 mice, tamoxifen administration caused the transient translocation of CreERT2 to the nucleus, allowing for the recombination of loxP-flanked sequences. In our system, tamoxifen administration at E14.5 resulted in reporter gene activation throughout the developing CNS of transgenic embryos. In the adult CNS, neurogenic regions were the primary sites of tamoxifen-induced reporter gene activation. In addition, reporter expression could also be detected outside of neurogenic regions in cells physiologically expressing DCX (e. g. piriform cortex, corpus callosum, hypothalamus). Four weeks after recombination, the vast majority of reporter-expressing cells were found to co-express NeuN, revealing the neuronal fate of DCX+ cells upon maturation. Conclusions: This first validation demonstrates that our new DCX-CreERT2 transgenic mouse model constitutes a powerful tool to investigate neurogenesis, migration and their long-term fate of neuronal precursors. Moreover, it allows for a targeted activation or deletion of specific genes in neuronal precursors and will thereby contribute to unravel the molecular mechanisms controlling neurogenesis.

NetApp's Chief Marketing Officer, Jay Kidd, and Brocade's Senior Vice President of Worldwide Sales, Ian Whiting, discuss NetApp's introduction of Brocade's FCoE product portfolio, which include a top-of-rack switch, converged network adapters, and FCoE blade for the DCX backbone.

NetApp's Chief Marketing Officer, Jay Kidd, and Brocade's Senior Vice President of Worldwide Sales, Ian Whiting, discuss NetApp's introduction of Brocade's FCoE product portfolio, which include a top-of-rack switch, converged network adapters, and FCoE blade for the DCX backbone.

Auf der Suche nach bislang unbekannten Proteinen des Centrosoms von Dictyostelium wurde zunächst auf der Ebene des Dictyostelium-Genomprojekts, basierend auf Ähnlichkeiten zu bekannten centrosomalen Proteinen anderer Spezies, nach möglichen Kandidaten gesucht. Zu den ca. 120 wahrscheinlich centrosomalen Proteinen in Tieren konnten hier nur 38 Homologe gefunden werden. Allerdings besteht das Dictyostelium-Centrosom wahrscheinlich aus ähnlich vielen verschiedenen Proteinen, sodass mit dieser Methode ein Großteil unentdeckt blieb. In einem Proteomics-Ansatz mit verschiedenen Auftrennungsmethoden wurde das Dictyostelium-Centrosom systematisch untersucht. Hierfür wurde zunächst ein Verfahren erarbeitet, Centrosomen in hinreichender Reinheit für massenspektrometrische Analysen zu präparieren. Am Ende der Bemühungen wurden 33 neue mögliche centrosomale Proteine gefunden, von denen bereits drei bestätigt werden konnten. Parallel wurde im Dictyostelium-System die Krankheit Lissenzephalie untersucht, eine Migrationsstörung von Neuronen bei der Gehirnentwicklung, bei der Centrosom-assozierte Proteine eine wichtige Rolle spielen. Zellmotilität und Entwicklung sind in Dictyostelium besonders gut zu beobachten, außerdem existieren hier Homologe zu den miteinander interagierenden Proteinen LIS1 und DCX, deren Mutationen beim Menschen Lissenzephalie auslösen. Mit DdDCX wurde ein Homologes (29 % Identität) zum humanen DCX gefunden und unter Einsatz von Fusionsproteinen und eines Antikörpers umfangreich charakterisiert. DdDCX bindet an Mikrotubuli und wird hauptsächlich in der Aggregationsphase exprimiert. Die generierte Nullmutante zeigte jedoch keinen Phänotyp. Das centrosomale Protein DdLIS1 hat zahlreiche wichtige Dynein-assoziierte Funkionen in vegetativ wachsenden Zellen. Hier konnte in durch gezielte Mutationen gezeigt werden, dass DdLIS1 eine Rolle bei der Entwicklung spielt, auch wenn es selbst nicht entwicklungsreguliert ist. Eine klare Aussage wurde erst durch die Generierung einer Doppelmutante möglich: Bei dieser ist die Aggregation in der Entwicklung gestört, also die Phase, in der wie bei Neuronen Zellbewegung und die Kommunikation zwischen den Zellen besonders wichtig sind. Da gezeigt werden konnte, dass Mikrotubuli dafür nicht essentiell sind, sind Spekulationen über gestörte Mikrotubuli-Dynamik als Ursache für die Migrationsstörung in Dictyostelium nicht haltbar. Mögliche Erklärungen bieten dagegen die nachgewiesene Interaktion mit Aktin oder die Beteiligung von LIS1 an der Regulation von PAF, einem intrazellulären Botenstoff, der auch in Neuronen eine Rolle spielt.