Podcasts about Metabolite

Play Episode Listen Later Mar 29, 2023

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2023.03.29.534678v1?rss=1 Authors: Sirucek, L., Zoelch, N., Schweinhardt, P. Abstract: Purpose: Functional understanding of the periaqueductal grey (PAG), a physiologically and clinically highly relevant brainstem region, can be advanced using proton magnetic resonance spectroscopy (1H-MRS). However, the PAG's small size and high levels of physiological noise are methodologically challenging. This study aimed to (1) improve 1H-MRS data quality in the PAG using spectral registration for frequency and phase error correction, (2) investigate whether spectral registration is particularly useful in cases of greater head motion and (3) examine two metabolite quantification approaches using literature-based or individual-based water relaxation times. Methods: Spectra were acquired in 33 healthy volunteers (50.1 years, SD=17.19, 18 females) on a 3T Philipps MR system using a point-resolved spectroscopy sequence optimized with very selective saturation pulses (OVERPRESS) and voxel-based flip angle calibration (effective volume of interest size: 8.8x10.2x12.2 mm3). Results: Spectral registration significantly improved signal-to-noise ratios (SNR; p=0.048, median value [interquartile range]: 18.0 [17.00-20.00]) and spectral linewidth (N-acetylaspartate peak: p less than 0.001, 4.3 Hz [3.96-4.85]), as well as metabolite fit (p's less than 0.046) compared to post-processing with minimal frequency alignment using interleaved unsuppressed water peaks. Correlational analyses revealed smaller improvements in SNR in cases of greater head motion (p=0.002) recorded with a markerless motion tracking system. Metabolite concentrations were higher when quantified using individual-based compared to literature-based water relaxation times (p's less than 0.001). Conclusion: This study demonstrates that high-quality 1H-MRS can be acquired in the PAG using spectral registration. This offers new opportunities for future 1H-MRS studies in the PAG and possibly also other clinically relevant brain regions with similar methodological challenges. Copy rights belong to original authors. Visit the link for more info Podcast created by Paper Player, LLC

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FDX1 regulates cellular protein lipoylation through direct binding to LIAS.

Play Episode Listen Later Feb 4, 2023

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2023.02.03.526472v1?rss=1 Authors: Dreishpoon, M., Bick, N., Petrova, B., Warui, D. M., Cameron, A., Booker, S. J., Kanarek, N., Golub, T., Tsvetkov, P. Abstract: Ferredoxins are a family of iron-sulfur (Fe-S) cluster proteins that serve as essential electron donors in numerous cellular processes that are conserved through evolution. The promiscuous nature of ferredoxins as electron donors enables them to participate in many metabolic processes including steroid, heme, vitamin D and Fe-S cluster biosynthesis in different organisms. However, the unique natural function(s) of each of the two human ferredoxins (FDX1 and FDX2) are still poorly characterized. We recently reported that FDX1 is both a crucial regulator of copper ionophore induced cell death and serves as an upstream regulator of cellular protein lipoylation, a mitochondrial lipid-based post translational modification naturally occurring on four mitochondrial enzymes that are crucial for TCA cycle function. Here we show that FDX1 regulates protein lipoylation by directly binding to the lipoyl synthase (LIAS) enzyme and not through indirect regulation of cellular Fe-S cluster biosynthesis. Metabolite profiling revealed that the predominant cellular metabolic outcome of FDX1 loss-of-function is manifested through the regulation of the four lipoylation-dependent enzymes ultimately resulting in loss of cellular respiration and sensitivity to mild glucose starvation. Transcriptional profiling of cells growing in either normal or low glucose conditions established that FDX1 loss-of-function results in the induction of both compensatory metabolism related genes and the integrated stress response, consistent with our findings that FDX1 loss-of-functions is conditionally lethal. Together, our findings establish that FDX1 directly engages with LIAS, promoting cellular protein lipoylation, a process essential in maintaining cell viability under low glucose conditions. Copy rights belong to original authors. Visit the link for more info Podcast created by Paper Player, LLC

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The dietary polyphenol metabolite protocatechuic acid produces acute and sustained effects on hippocampal electrical activity

Play Episode Listen Later Jan 13, 2023

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2023.01.12.523723v1?rss=1 Authors: Montero-Atalaya, M., Munoz-Arnaiz, R., Makarova, J., Bartolome, B., Moreno-Arribas, V., Herreras, O. Abstract: Dietary polyphenols and in particular bioavailable metabolites resulting from gut microbiota transformations appear to have beneficial effects in situations of impaired cognition, combatting memory deficits in acute pathological models of neurodegeneration. Modifications to blood flow may underlie the effects of these molecules and although some such metabolites cross the blood-brain barrier, their targets and electrophysiological effects remain unknown. Hence, we explored the systemic and direct effects of protochatechuic acid (PCA) on electrical activity in the hippocampus and cortex of anesthetized female rats, recording evoked and spontaneous high-density field potentials (FPs) to mathematically derive pathway-specific FP generators. We found transient and sustained effects of PCA on evoked activity in the CA1 field, including paradoxical actions on excitatory transmission that depend on the route of administration. Systemic delivery of PCA altered the ongoing activity of some FP generators, albeit with marked inter-animal variation. Interestingly, PCA induced the detachment of infraslow cortico-hippocampal activities over a scale of minutes. These results point to direct actions of polyphenols on cell and network electrical activity, some of which reflect non-specific actions. Thus, dietary-derived polyphenols appear to fulfill neuromodulatory roles, encouraging the search for additional targets to better guide their use in preventing brain pathologies. Copy rights belong to original authors. Visit the link for more info Podcast created by Paper Player, LLC

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Metabolite T1 relaxation times differ across the adult lifespan

Play Episode Listen Later Jan 8, 2023

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2023.01.06.522927v1?rss=1 Authors: Murali-Manohar, S., Gudmundson, A. T., Hupfeld, K. E., Zöllner, H. J., Hui, S. C. N., Song, Y., Davies-Jenkins, C. W., Gong, T., Wang, G., Oeltzschner, G., Edden, R. A. E. Abstract: Purpose: To investigate the age-dependence of metabolite T1 relaxation times at 3T in both gray- and white-matter-rich voxels. Methods: This manuscript analyzes publicly available metabolite and metabolite-nulled (single inversion recovery TI = 600 ms) spectra acquired at 3T using PRESS localization. Voxels were placed in posterior cingulate cortex and centrum semiovale in 102 healthy volunteers across 5 decades of life (20s to 60s). All spectra were analyzed in Osprey v2.4.0. To estimate T1 relaxation times for tNAA2.0 and tCr3.0, the ratio of modeled metabolite residual amplitudes in the metabolite-nulled spectrum to the full metabolite signal was calculated using the single inversion recovery signal equation. Correlations between T1 and subject age were evaluated. Results: Spearman correlations revealed that estimated T1 relaxation times of tNAA2.0 (rs = -0.43; p less than 0.001) and tCr3.0 (rs = -0.23; p = 0.021) decreased significantly with age in white-matter-rich CSO, and less steeply (and not significantly) for tNAA2.0 (rs = -0.15; p = 0.136) and tCr3.0 (rs = -0.10; p = 0.319) in gray-matter-rich PCC. Conclusion: The analysis harnessed a large publicly available cross-sectional dataset to test an important hypothesis, that metabolite T1 relaxation times change with age. This preliminary study stresses the importance of further work to measure age-normed metabolite T1 relaxation times for accurate quantification of metabolite levels in studies of aging. Copy rights belong to original authors. Visit the link for more info Podcast created by Paper Player, LLC

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A metabolite sensor subunit of the Atg1/ULK complex regulates selective autophagy

Play Episode Listen Later Dec 14, 2022

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2022.12.13.520293v1?rss=1 Authors: Gross, A., Ghillebert, R., Schuetter, M., Reinartz, E., Rowland, A., Graef, M. Abstract: Cells convert complex metabolic information into stress-adapted autophagy responses. Canonically, multilayered protein kinase networks converge on the conserved Atg1/ULK kinase complex (AKC) to induce non-selective and selective forms of autophagy in response to metabolic changes. Here, we show that, upon phosphate starvation, the metabolite sensor Pho81 interacts with the adaptor subunit Atg11 at the AKC via an Atg11/FIP200 interaction motif to modulate pexophagy by virtue of its conserved phospho-metabolite sensing SPX domain. Notably, we find core AKC components Atg13 and Atg17 are dispensable for phosphate starvation-induced autophagy revealing significant compositional and functional plasticity of the AKC. Our data indicate that, instead of functioning as a selective autophagy receptor, Pho81 compensates for partially inactive Atg13 during pexophagy when TORC1 remains active under phosphate starvation. Our work shows Atg11/FIP200 adaptor subunits not only bind selective autophagy receptors but also modulator subunits that convey metabolic information directly to the AKC for autophagy regulation. Copy rights belong to original authors. Visit the link for more info Podcast created by Paper Player, LLC

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Changes in brain metabolite levels across childhood

Play Episode Listen Later Dec 1, 2022

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2022.11.30.518618v1?rss=1 Authors: Perdue, M. V., DeMayo, M. M., Bell, T., Boudes, E., Bagshawe, M., Harris, A. D., Lebel, C. Abstract: Metabolites play important roles in brain development and their levels change rapidly in in the prenatal period and during infancy. Metabolite levels are thought to stabilize during childhood, but the development of neurochemistry across early-middle childhood remains understudied. We examined the developmental changes of key metabolites (total N- acetylaspartate, tNAA; total choline, tCho; total creatine, tCr; glutamate+glutamine, Glx; and myo-inositol, mI) using short echo-time magnetic resonance spectroscopy (MRS) in the anterior cingulate cortex (ACC) and the left temporo-parietal cortex (LTP) using a mixed cross- sectional/longitudinal design in children aged 2-11 years (ACC: N=101 children, 112 observations; LTP: N=95 children, 318 observations). We found age-related effects for all metabolites. tNAA increased with age in both regions, while tCho decreased with age in both regions. tCr increased with age in the LTP only, and mI decreased with age in the ACC only. Glx did not show linear age effects in either region, but a follow-up analysis in only participants with greater than or equal to 3 datapoints in the LTP revealed a quadratic effect of age following an inverted U-shape. These substantial changes in neurochemistry throughout childhood likely underlie various processes of structural and functional brain development. Copy rights belong to original authors. Visit the link for more info Podcast created by Paper Player, LLC

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Golgi-IP, a novel tool for multimodal analysis of Golgi molecular content

The Other Side of Weight Loss

Play Episode Listen Later Nov 23, 2022

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2022.11.22.517583v1?rss=1 Authors: Fasimoye, R., Dong, W., Nirujogi, R. S., Rawat, E. S., Iguchi, M., Nyame, K., Phung, T. K., Bagnoli, E., Prescott, A. R., Alessi, D. R., Abu-remaileh, M. Abstract: The Golgi is a membrane-bound organelle that is essential for protein and lipid biosynthesis. It represents a central trafficking hub that sorts proteins and lipids to various destinations or for secretion from the cell. The Golgi has emerged as a docking platform for cellular signalling pathways including LRRK2 kinase whose deregulation leads to Parkinson disease. Golgi dysfunction is associated with a broad spectrum of diseases including cancer, neurodegeneration, and cardiovascular diseases. To allow the study of the Golgi at high resolution, we report a rapid immunoprecipitation technique (Golgi-IP) to isolate intact Golgi mini-stacks for subsequent analysis of their content. By fusing the Golgi resident protein TMEM115 to three tandem HA epitopes (GolgiTAG), we purified the Golgi using Golgi-IP with minimal contamination from other compartments. We then established an analysis pipeline using liquid chromatography coupled with mass spectrometry to characterize the human Golgi proteome, metabolome and lipidome. Subcellular proteomics confirmed known Golgi proteins and identified novel ones. Metabolite profiling established the first known human Golgi metabolome and revealed the selective enrichment of uridine-diphosphate (UDP) sugars and their derivatives, which is consistent with their roles in protein and lipid glycosylation. Furthermore, targeted metabolomics validated SLC35A2 as the subcellular transporter for UDP-hexose. Finally, lipidomics analysis showed that phospholipids including phosphatidylcholine, phosphatidylinositol and phosphatidylserine are the most abundant Golgi lipids and that glycosphingolipids are enriched in this compartment. Altogether, our work establishes a comprehensive molecular map of the human Golgi and provides a powerful method to study the Golgi with high precision in health and disease states. Copy rights belong to original authors. Visit the link for more info Podcast created by Paper Player, LLC

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Urine Metabolite Testing for Hormones & What it Tells You, Who It is for & Why It is The Gold Standard of Testing with Karen Martel & Cohost Dr. Amie Hornaman

Play Episode Listen Later Oct 22, 2022 77:38

In this episode, my cohost Dr. Amie Hornaman and I go through her urine hormone metabolite test results. Whether you are a practitioner or lay person this show will teach you in great detail exactly what the urine metabolite hormone test can show you about your hormonal profile. You will learn what is the difference between urine, blood and saliva hormone testing what each marker can tell you in a urine test about your hormone levels and how you break them down what urine testing can tell you about your metabolism and thyroid function the different nuances between urine and serum and why they may not add up what urine testing can tell you about your ability to detoxify and which pathway may be holding you up what markers can show us that we may have an increased risk of developing osteoporosis, breast cancer, acne and more what it means to be an alpha hormone metabolizer versus a beta who the urine test is best for how it can help you personalize your hormone replacement therapy to get the most out of it Amie's blood labs compared with the DUTCH test and the discrepancies it showed the cheapest option you have when buying a urine metabolite test and more!! Watch the video of this episode here. Check out Dr. Amie Hornaman and her shop here and get 10% off your order with coupon code karen Get $20 off your advanced urine metabolite test with coupon code karen20. The coupon expires on Oct 31st. Do you have a question for our next Q&A? Please fill out this form. Get the details and apply to be part of my 3-month Mastering Peri & Post Menopause coaching program. Start your membership to the OnTrack group coaching program to help you balance your hormones and lose weight. Karen Martel, Certified Hormone Specialist & Transformational Nutrition Coach and weight loss expert. Visit https://karenmartel.com/ Karen's Facebook Karen's Instagram *I have teamed up with businesses and products that I love and may receive compensation for products I review on this site at no additional cost to you.

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Metabolite profiling and cytotoxic activity of Andean potatoes: polyamines and glycoalkaloids as potential anticancer agents in human neuroblastoma cells in vitro

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Play Episode Listen Later Oct 14, 2022

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2022.10.13.512083v1?rss=1 Authors: Lanteri, M. L., Silveyra, M. X., Moran, M. M., Boutet, S., Solis-Gozar, D.-D., Perreau, F., Andreu, A. B. Abstract: Andean potatoes (Solanum tuberosum L. ssp. andigena) are a good source of dietary antioxidant polyphenols. We have previously demonstrated that polyphenol extracts from Andean potato tubers exerted a dose-dependent cytotoxic effect in human neuroblastoma SH-SY5Y cells, being skin extracts more potent than flesh ones. In order to gain insight into the bioactivities of potato phenolics, we investigated the composition and the in vitro cytotoxic activity of total extracts and fractions of skin and flesh tubers of three Andean potato cultivars (Santa Maria, Waicha, and Moradita). Potato total extracts were subjected to liquid-liquid fractionation using ethyl acetate solvent in organic and aqueous fractions. We analyzed both fractions by HPLC-DAD, HPLC-ESI-MS/MS, and HPLC-HRMS. Results corroborated the expected composition of each fraction. Organic fractions were rich in hydroxycinnamic acids (principally chlorogenic acid isomers), whereas aqueous fractions contained mainly polyamines conjugated with phenolic acids, glycoalkaloids, and flavonoids. Organic fractions were not cytotoxic against SH-SY5Y cells, and indeed, some increased cellular metabolism compared to controls. Aqueous fractions were cytotoxic and even more potent than their respective total extracts. Treatment with a combination of both fractions showed a similar cytotoxic response to the corresponding extract. According to correlation studies, it is tempting to speculate that polyamines and glycoalkaloids are crucial in inducing cell death. Our findings indicate that the activity of Andean potato extracts is a combination of various compounds and contribute to the revalorization of potato as a functional food. Copy rights belong to original authors. Visit the link for more info Podcast created by Paper Player, LLC

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The Gary Null Show - 09.22.22

The Gary Null Show

Play Episode Listen Later Sep 22, 2022 56:42

VIDEOS: The elite that has taken almost all the money is now after everything else as well | Neil Oliver The Metaverse Is Worse Than You Thought – by MOON 11 reasons an annual COVID-19 booster is NOT LIKE an annual flu shot Blackcurrant nectar shows exercise benefits for college students: Study University of the Incarnate Word, September 14, 2022 Daily consumption of blackcurrant nectar for eight days may reduce muscle damage and inflammation after exercise, according to a new study from scientists at the University of the Incarnate Word in San Antonio, Texas. Sixteen ounces per day of the blackcurrant nectar were associated with reductions in the activity of creatine kinase, a blood marker of muscle damage, by 6.7%, compared to 82% increases in activity in the placebo group 48 hours after exercise, report the researchers in the Journal of Dietary Supplements . Researchers led by Alexander Hutchison, PhD, also report that levels of the inflammatory compound interleukin-6 decreased after exercise in participants in the blackcurrant group, compared with increases seen in the placebo group. “In partial support of our primary hypotheses, we found that consumption of black currant nectar for four days before and three days after a bout of eccentric leg exercise significantly reduced circulating markers of muscle damage while maintaining circulating antioxidant capacity,” they wrote in their paper. “Although pain scores in the blackcurrant nectar group returned to baseline a day earlier than the placebo group, there were no significant differences observed between groups at any time point after exercise. The study included 16 college students randomly assigned to consumer either the blackcurrant nectar beverage (CurrantC provided by CropPharms from Staatsburg, NY) or placebo twice a day for eight days. On day 4 the participants performed a bout of knee extension exercises, and blood samples taken 24, 48, and 96 hours after the exercise. Results showed that ORAC levels in the blood significantly decreased in the placebo group, while no significant decreases from the baseline values were observed in the blackcurrant group. In addition, significant differences between the groups were observed for IL-6 levels 24 hours after exercise, while significant differences were observed in creatine kinase activity between the groups after 48 and 96 hours.. How does what we eat affect our health span and longevity? It's a complex, dynamic system Columbia University's Mailman School of Public Health, September 21, 2022 How does what we eat affect how we age? The answer to this relatively concise question is unavoidably complex, according to a new study at the Butler Columbia Aging Center at Columbia University Mailman School of Public Health. The findings are published online in the journal BMC Biology. While most analyses had been concerned with the effects of a single nutrient on a single outcome, a conventional, unidimensional approach to understanding the effects of diet on health and aging no longer provides us with the full picture: A healthy diet must be considered based on the balance of ensembles of nutrients, rather than by optimizing a series of nutrients one at a time. Until now little was known about how normal variation in dietary patterns in humans affects the aging process. “”This study therefore provides further support to the importance of looking beyond ‘a single nutrient at a time' as the one size fits all response to the age-old question of how to live a long and healthy life.” Cohen also points that the results are also concordant with numerous studies highlighting the need for increased protein intake in older people, in particular, to offset sarcopenia and decreased physical performance associated with aging. The researchers analyzed data from 1,560 older men and women, aged 67-84 years selected randomly from the Montreal, Laval, or Sherbrooke areas in Quebec, Canada, who were re-examined annually for three years and followed over four years to assess on a large scale how nutrient intake associates with the aging process. Aging and age-related loss of homeostasis (physiological dysregulation) were quantified via the integration of blood biomarkers. The effects of diet used the geometric framework for nutrition, applied to macronutrients and 19 micronutrients/nutrient subclasses. Researchers fitted a series of eight models exploring different nutritional predictors and adjusted for income, education level, age, physical activity, number of comorbidities, sex, and current smoking status. Four broad patterns were observed: The optimal level of nutrient intake was dependent on the aging metric used. Elevated protein intake improved/depressed some aging parameters, whereas elevated carbohydrate levels improved/depressed others; There were cases where intermediate levels of nutrients performed well for many outcomes (i.e. arguing against a simple more/less is better perspective); There is broad tolerance for nutrient intake patterns that don't deviate too much from norms (“homeostatic plateaus”). Optimal levels of one nutrient often depend on levels of another (e.g. vitamin E and vitamin C). Simpler analytical approaches are insufficient to capture such associations. Mediterranean diet could play a key role in preventing cognitive decline Brigham and Women's Hospital and Harvard University, September 20, 2022 Individuals of minoritized ethnic or racial groups are often underrepresented in research, thus hindering the understanding of risk factors and the efficacy of treatments for diseases in these minoritized groups. A recent study published in the journal Alzheimer's & Dementia found that the levels of six plasma metabolites were associated with lower cognitive function across all racial/ethnic groups, and the levels of most of these blood metabolites were associated with adherence to a Mediterranean diet. Speaking to Medical News Today, the study's corresponding author Dr. Tamar Sofer, a professor at Brigham and Women's Hospital at Harvard University, said: “We identified a few metabolites (small molecules) in blood that their levels are correlated with cognitive function, and they are all related to diet. Characterizing metabolites associated with cognitive function can help researchers understand the mechanisms underlying the development of dementia. Moreover, blood metabolites can be easily measured and could serve as biomarkers for cognitive function. A previous study involving older Puerto Rican individuals showed that the levels of 13 blood metabolites were associated with global cognitive function, which is a composite measure of multiple cognitive abilities. Metabolite levels are influenced by the interplay between genetics, health status, and environmental factors, including diet, other lifestyle factors, and socioeconomic factors, which may differ among and even within ethnic/racial groups. The meta-analysis showed that six blood metabolites were associated with lower cognitive function across all ethnic/racial groups. Four out of the six metabolites associated with overall cognitive function were sugars, including glucose, ribitol, mannose, and mannitol/sorbitol. Out of the six metabolites, the analysis revealed a potential causal effect of only ribitol on cognitive function. The researchers also assessed the association between dietary habits, including adherence to a Mediterranean diet and intake of food groups (i.e. intake of legumes, fruits, vegetables, meat, fish, etc.), and blood metabolite levels. They found that adhering to a Mediterranean diet or its component food groups was correlated with several blood metabolites assessed in the study. Notably, the strongest association was observed between beta-cryptoxanthin and fruit intake participants. Beta-cryptoxanthin is a carotenoid with antioxidant properties found in fruits and vegetables, and beta-cryptoxanthin levels are associated with a lower risk of insulin resistance and liver dysfunction. “[T]his study is a step in the right direction in relation to examining the role of diet and the body's metabolism for brain health. It provides suggestive evidence that adherence to a good diet such as the Mediterranean style diet may be beneficial for brain health over a wide age range.” Indigo Rose Tomatoes Contain An Antioxidant That Fights Diabetes, Cancer and Neurodegenerative Diseases Oregon State University, September 15, 2022 Not only do dark tomatoes turn heads, but they are also healthier than normal red varieties, according to plant scientists. Indigo Rose Tomatoes were cultivated by breeding red and purple tomato plants, and are being heralded as a new superfood with potent antioxidants. Scientists bred purple tomatoes containing anthocyanin, an antioxidant said to help fight several diseases, with normal red varieties. ‘There are some dark coloured tomatoes but Indigo Rose is the only real black tomato and is the darkest that has ever been bred. “It's not genetically modified or GMO-based as many assume,” said Botanist Marjorie Varga. “People often get confused between GMO and hybridization which farmers have been using to cultivate new plant varieties for thousands of years.” “It is the first improved tomato variety in the world that has anthocyanins in its fruit,” he said. Myers' team found some tomatoes with purple pigmentation and tests revealed that anthocyanins were providing the colour, the same as blueberries. They crossed the purple tomatoes with some wild tomatoes and eventually came up with a black strain. Foot massage effective in improving sleep quality and anxiety in postmenopausal women Çankiri Karatekin University (Turkey), September 21, 2022 The therapeutic benefits of massage have long been recognized. A new study suggests that foot massage, in particular, can help minimize a number of common menopause symptoms, including sleep disruption, effectively extending sleep duration by an average of an hour per day. Study results are published online today in Menopause. During the menopause transition, estrogen deficiency can lead to a number of physical and mental health problems, including insomnia, hot flashes, vaginal dryness, headaches and anxiety. Although hot flashes and negative moods commonly seen in in the menopause transition often improve, conditions such as sleep complaints and vaginal dryness tend to persist or worsen over time. Previous studies have suggested that foot reflexology is an effective intervention in reducing stress and fatigue in premenopausal women. However, no previous studies were found that evaluated the effects of foot massage on anxiety, fatigue and sleep at the same time in postmenopausal women. In this new, small-scale study, researchers specifically sought to evaluate the effects of foot massage on anxiety, fatigue and sleep in postmenopausal women. Study results determined that foot massage applied during menopause increases the average daily sleep duration—as much as an hour per day—and reduces women's fatigue and anxiety levels. “Sleep disturbances, fatigue and anxiety symptoms are common during menopause. This small study in Turkish women shows how a simple, inexpensive intervention such as foot massage can improve these bothersome symptoms in postmenopausal women. Lack of sleep negatively impacts immune stem cells, increasing risk of inflammatory disorders and heart disease Icahn School of Medicine at Mount Sinai, September 21, 2022 Chronic insufficient sleep can negatively affect immune cells, which may lead to inflammatory disorders and cardiovascular disease, according to a new study from the Icahn School of Medicine at Mount Sinai. More specifically, consistently losing an hour and a half of sleep a night potentially increases the risk. The research, published in the Journal of Experimental Medicine, is the first to show that sleep alters the structure of DNA inside the immune stem cells that produce white blood cells—also known as immune cells—and this can have a long-lasting impact on inflammation and contribute to inflammatory diseases. Immune cells fight infection, but if the number of these cells gets too high, they overreact and cause inflammation. The study is also the first to show that catching up on sleep doesn't reverse the effects of sleep disruption. “This study begins to identify the biological mechanisms that link sleep and immunological health over the long-term. It shows that in humans and mice, disrupted sleep has a profound influence on the programming of immune cells and rate of their production, causing them to lose their protective effects and actually make infections worse—and these changes are long-lasting. This is important because it is yet another key observation that sleep reduces inflammation and, conversely, that sleep interruption increases inflammation,” says lead author Filip Swirski, Ph.D., Director of the Cardiovascular Research Institute at Icahn Mount Sinai. “This work emphasizes the importance of adults consistently sleeping seven to eight hours a day to help prevent inflammation and disease, especially for those with underlying medical conditions.” A team of investigators analyzed 14 healthy adults who regularly sleep eight hours a night. First, researchers monitored them sleeping at least eight hours a night for six weeks. They drew their blood and analyzed their immune cells. Then, the same group of adults reduced their sleep time by 90 minutes every night for six weeks, and had their blood and immune cells reanalyzed. At the end of the study researchers compared the blood and cell samples from the full night's sleep and restricted sleep periods. All participants had significant changes in their immune cells (also known as hematopoietic cells) due to a lack of sleep—there were more of them, and the DNA structure was altered. After six weeks of sleep restriction, they had an increased number of immune cells. Results in humans showed that fragmented sleep had significant changes to their immune stem cells, producing an increased number of immune cells, and also showed evidence of rewiring and reprogramming. A notable finding from the mouse group was that even after sleep recovery, the immune stem cells retained this rewiring structure, and they continued to produce additional white blood cells, making the mice susceptible to inflammation and disease. “Our findings suggest that sleep recovery is not able to fully reverse the effects of poor-quality sleep. We can detect a molecular imprint of insufficient sleep in immune stem cells, even after weeks of recovery sleep. This molecular imprint can cause the cells to respond in inappropriate ways leading to inflammation and disease,” says co-lead investigator Cameron McAlpine, Ph.D., Assistant Professor of Medicine (Cardiology) at Icahn Mount Sinai.

An Exercise Metabolite That Can Reduce Food Intake and Induce Fat Loss

The People's Scientist

Play Episode Listen Later Jul 24, 2022 31:35

In Episode 130 I dive into a recent publication by Li et al. published this year in Nature that identified an exercise metabolite, lac-phe, that reduces food intake and induces fat loss. The scientists conducted a series of experiments to illustrate that certain types of exercise increase circulating lac-phe in the blood which is likely the mechanism behind reduced appetite, food intake, and enhanced fat loss. Tune in for details!Reference: Li, V.L., He, Y., Contrepois, K. et al. An exercise-inducible metabolite that suppresses feeding and obesity. Nature 606, 785–790 (2022). https://doi.org/10.1038/s41586-022-04828-5Want to buy me a coffee to help support the show? You can do so via the links below:Venmo ID: Steph-CalPatreon: https://www.patreon.com/join/DrSCaligiuriBuy Me a Coffee: https://www.buymeacoffee.com/drscaligiuriFollow me on social media to see the papers I cite in this week's episode:IG: Dr.SCaligiuriFB: ThePeoplesScientistTwitter: DrSCaligiuriLinkedin: Stephanie CaligiuriTikTok: Dr.SCaligiuri See acast.com/privacy for privacy and opt-out information.

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The Metabolomist - Metabolomics2022 Feature

The Metabolomist podcast

Play Episode Listen Later Jul 5, 2022 33:22

This episode features exclusive interviews collected at the 2022 Metabolomics Society conference in Valencia, Spain.

306: Should You Do Banded Exercises w/ Mike Israetel & Kassem Hanson

Play Episode Listen Later Apr 11, 2022 98:18

Mike & Kassem are back on the show together to talk more about biomechanics. In today's show, we dig into the utility of bands for adjusting resistance profiles, specifically for exercises like the hack squat. As always guys lots of detail and nuance to be found in this chat along with some practical take-homes. Timestamps: (00:00) Intro (01:55) Is banding worth it? Banding on hack squats (20:04) How to quantify the benefits of banding (28:56) Sticking point on a hack squat & rationale to band the hack squats (43:04) Argument for banding exercises and how to do it (51:01) Stimulus to fatigue ratio when banding (01:01:45) Time consumption (01:09:14) Making movements easier/harder (01:14:55) Metabolite work and defeating the purpose of banding (01:22:56) Conclusion https://www.instagram.com/coach_kassem/ https://www.instagram.com/N1.education/ https://www.instagram.com/drmikeisraetel/ https://www.instagram.com/rpstrength/ Thanks, please comment, like and subscribe! COACHING: https://revivestronger.com/online-coaching/ MEMBERSITE: https://revivestronger.com/team-revive-stronger/ WEBSITE: https://www.revivestronger.com FACEBOOK: http://www.facebook.com/revivestronger INSTAGRAM: http://www.instagram.com/revivestronger NEWSLETTER: https://bit.ly/2rRONG5 YOUTUBE: https://www.youtube.com/watch?v=fluVEKr2UV0 __________________________________________________________________ If you want to support us via a donation, that's highly appreciated! Patreon • https://www.patreon.com/revivestronger Don't like Patreon, go to Paypal! • https://bit.ly/2XZloJ4 __________________________________________________________________ Our Ebooks! Ultimate Guide To Contest Prep Ebook: • https://revivestronger.com/product/the-ultimate-guide-to-contest-prep/ Primer Phase Ebook: • https://revivestronger.com/product/the-primer-phase/ __________________________________________________________________ Stay up to date with the latest research and educate yourself! MASS (Research Review): • https://goo.gl/c7FSJD RP+ Membership: • https://ob262.isrefer.com/go/plus/Steve90/ JPS Mentorship • https://jpseducation.mykajabi.com/a/13324/esJ8AZwy __________________________________________________________________ Books we recommend! Muscle & Strength Pyramids • https://goo.gl/S8s6tG RP Books • http://bit.ly/2vREaH0 RP + Members site • https://ob262.isrefer.com/go/plus/Steve90/ For more • http://revivestronger.com/library/ __________________________________________________________________ Supplements: www.cnpprofessional.co.uk - STEVE15 for 15% off __________________________________________________________________ When you're interested in online coaching, please go visit our website and follow the application form: https://www.revivestronger.com/online-coaching/

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297: Brad Schoenfeld - How To Periodise For Max Muscle Growth

Lupus Science and Medicine podcast

Play Episode Listen Later Feb 7, 2022 59:00

Brad is back on the show for his annual appearance. In this episode, we dig into the question of whether you should cut volume to preserve muscle in a cut if partial repetitions can be beneficial for hypertrophy, and questions surrounding the periodised programme within his recent book release. Timestamps: (00:00) Intro (06:01) Differences between Max Muscle Plan version 1 & 2. Nutrient timing (10:48) What's a true evidence-based practitioner (12:30) Who is the book for? (16:30) Volume and preserving muscle mass (22:11) Full ROM vs. Partial ROM (31:16) Is variation important for hypertrophy? (35:26) How to periodise for max muscle growth (39:56) Metabolite training for hypertrophy (42:37) Combine all things at once? (44:30) Is failure necessary when you train with higher reps? (45:43) Importance of exercise order (51:00) Specialisation phases necessary with training experience and what's junk volume https://www.instagram.com/bradschoenfeldphd/ https://www.amazon.com/M-X-Muscle-Plan-2-0/dp/171820714X/ http://www.lookgreatnaked.com/about_brad.php Thanks, please comment, like and subscribe! COACHING: https://revivestronger.com/online-coaching/ MEMBERSITE: https://revivestronger.com/team-revive-stronger/ WEBSITE: https://www.revivestronger.com MINI CUT MOVEMENT: https://revivestronger.com/mini-cut-movement/ FACEBOOK: http://www.facebook.com/revivestronger INSTAGRAM: http://www.instagram.com/revivestronger NEWSLETTER: https://bit.ly/2rRONG5 YOUTUBE: https://www.youtube.com/watch?v=J0sikTfehbk __________________________________________________________________ If you want to support us via a donation, that's highly appreciated! Patreon • https://www.patreon.com/revivestronger Don't like Patreon, go to Paypal! • https://bit.ly/2XZloJ4 __________________________________________________________________ Our Ebooks! Ultimate Guide To Contest Prep Ebook: • https://revivestronger.com/product/the-ultimate-guide-to-contest-prep/ Primer Phase Ebook: • https://revivestronger.com/product/the-primer-phase/ __________________________________________________________________ Stay up to date with the latest research and educate yourself! MASS (Research Review): • https://goo.gl/c7FSJD RP+ Membership: • https://ob262.isrefer.com/go/plus/Steve90/ JPS Mentorship • https://jpseducation.mykajabi.com/a/13324/esJ8AZwy __________________________________________________________________ Books we recommend! Muscle & Strength Pyramids • https://goo.gl/S8s6tG RP Books • http://bit.ly/2vREaH0 RP + Members site • https://ob262.isrefer.com/go/plus/Steve90/ For more • http://revivestronger.com/library/ __________________________________________________________________ __________________________________________________________________ When you're interested in online coaching, please go visit our website and follow the application form: https://www.revivestronger.com/online-coaching/

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RP Coach Hunter Pauley

RP Strength Podcast

Play Episode Listen Later Jan 24, 2022 61:08

0:28 How Hunter became an RP coach 3:30 Hunter adapting to Montana life and weather 11:15 Hunter's struggles in highschool 15:45 Hunter's introduction to fitness 19:55 Hunter's education 24:34 How Hunter found RP 29:26 Managing lifting volume 33:45 Metabolite training 40:00 Montana hiking 42:45 Hunters coaching success stories 48:00 Meal prep and diet tips 52:12 What Hunter gives to his clients 58:58 How to find Hunter

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Microbial Metabolite Mimicry May Improve Drug Discovery

Oncotarget

Play Episode Listen Later Jan 17, 2022 17:23

Dr. Sridhar Mani from Medicine and Genetics of The Albert Einstein College of Medicine, Bronx, NY, details his editorial published by Oncotarget in Volume 11, Issue 19, entitled, “Microbial metabolite mimicry: one step closer to drug discovery.” DOI - https://doi.org/10.18632/oncotarget.27591 Correspondence to - Sridhar Mani - sridhar.mani@einsteinmed.org Sign up for free Altmetric alerts about this article - https://oncotarget.altmetric.com/details/email_updates?id=10.18632%2Foncotarget.27591 Keywords - microbial metabolites, inflammation, IBD, mimicry, drugs About Oncotarget Oncotarget is a peer-reviewed, open access biomedical journal covering research on all aspects of oncology. To learn more about Oncotarget, please visit https://www.oncotarget.com or connect with: SoundCloud - https://soundcloud.com/oncotarget Facebook - https://www.facebook.com/Oncotarget/ Twitter - https://twitter.com/oncotarget Instagram - https://www.instagram.com/oncotargetjrnl/ YouTube - https://www.youtube.com/c/OncotargetYouTube/ LinkedIn - https://www.linkedin.com/company/oncotarget Pinterest - https://www.pinterest.com/oncotarget/ Reddit - https://www.reddit.com/user/Oncotarget/ Oncotarget is published by Impact Journals, LLC please visit https://www.ImpactJournals.com or connect with @ImpactJrnls Media Contact MEDIA@IMPACTJOURNALS.COM 18009220957

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Personalised metabolite monitoring during pre-conception and pregnancy in SLE patients

Play Episode Listen Later Nov 17, 2021 18:15

Duane Peters from the Lupus Foundation of America interviews Francisca Lambert-Fliszar and Dr Evelyne Vinet from McGill University Health Centre in Canada. They discuss monitoring 6-mercaptopurine (6-MP) metabolite levels to enable personalisation of immunosuppressive therapies prior to conception or during pregnancy, in order to most effectively and safely control the disease during this critical time. Access the paper here: https://lupus.bmj.com/content/8/1/e000519

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463: Selectively Stimulating Cell Squatters

BacterioFiles

Play Episode Listen Later Sep 20, 2021 12:00

This episode: Bacteria produce a compound that causes a phage lurking in the genome of a competing species to wake up and start killing that competitor! Download Episode (8.2 MB, 12.0 minutes) Show notes: Microbe of the episode: Zaire ebolavirus News item Takeaways Some bacteriophages infect and immediately destroy their hosts in a burst of new viruses, while others can be stealthier, integrating their genome into the genome of the host and remaining there quietly even over multiple generations of the bacteria. When something stresses the host, such as DNA damage, these integrated phages (prophages) become active and start producing new viruses, killing their host like the other kind does. In this study, one kind of bacteria release a chemical that wakes up phages in a competitor species of bacteria. This is helpful for competition, but even more interesting is that out of the six prophages in the competitor species, the chemical wakes up only one of them. Such selective phage induction could be interesting to study. Journal Paper: Jancheva M, Böttcher T. 2021. A Metabolite of Pseudomonas Triggers Prophage-Selective Lysogenic to Lytic Conversion in Staphylococcus aureus. J Am Chem Soc 143:8344–8351. Other interesting stories: Lab-evolved fungus strain could protect honeybees from mites Email questions or comments to bacteriofiles at gmail dot com. Thanks for listening! Subscribe: Apple Podcasts, Google Podcasts, Android, or RSS. Support the show at Patreon, or check out the show at Twitter or Facebook.

Everything You Need To Know About Short-Chain Fatty Acids

The Microbiome Report

Play Episode Listen Later Aug 5, 2021 33:38

Typically, we don't think about picking up the waste byproducts of another species and using them to our advantage. But when it comes to short chain fatty acids - the metabolites of byproducts of our microbes - there's actually a lot we humans benefit from. On this episode, Stephen Fleming, president of Traverse Science, talks to Andrea about what short-chain fatty acids are, what they do, what they mean in the body, and where we can find them. Leave Us A Rating And Review! The Microbiome Report is powered by BIOHM Health. Save 15% off at biohmhealth.com with the code POD15. Find the transcript and show notes for this episode at biohmhealth.com/pages/podcast. Questions? Ideas? Email us at themicrobiomereport@biohmhealth.com or reach out on Instagram @DreEats or @BIOHMHealth.

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Metabolite Accumulation | Pre-fatiguing Tissue | New Cuffs | BFR Q&A

The Blood Flow Restriction (BFR) University Podcast

Play Episode Listen Later May 20, 2021 22:28

Join Dr. Ed Le Cara as he answers people's questions about BFR. In this session, he talks about metabolite accumulation, why and how you should pre fatigue tissue, what are the effects on the area distal to and proximal to the cuff, and what are the best effects. He also shares how BFR can help with osteoporotic or osteopenia and a lot more. For More: https://www.bfruniversity.com/

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Vigyan Patrika S2 EP15: RNA-based biosensors win a race for metabolite-sensing in cells

Bio Patrika Podcast

Play Episode Listen Later Apr 12, 2021 7:10

This episode is also available as a blog post: http://biopatrika.com/2021/04/12/interview-rna-biosensors-metabolite-sensing-cells/

race cells sensing biosensors patrika metabolite

247: Brad Schoenfeld - Training to failure for strength & hypertrophy

Clinical Chemistry Podcast

Play Episode Listen Later Feb 22, 2021 65:43

The hypertrophy king Dr Brad Schoenfeld is back on the show. In this episode we talk about a recent paper whereby a strength phase proceeded a hypertrophy phase & saw more gains, we also discussed Brad's recent meta-analysis on failure training and much more! Timestamps: 00:00 Intro 01:20 Sissy squats 03:46 Strength phases beneficial for hypertrophy? 08:07 Overreaching a good thing? 08:55 People dismissing science 13:52 The arguments of overthinking things 15:59 Training to failure for strength & hypertrophy 26:15 Argument of "You don't know how to train to failure" 29:58 What is the definition of training to failure 32:45 What is too easy of training? 37:08 Detrimental effects of systemic fatigue 43:49 Genetic ceiling 47:27 Max muscle plan, new version and what are differences 51:06 Periodisation and programme 54:46 Metabolite blocks 59:45 Dogmatism and black & white statements https://www.lookgreatnaked.com/ https://www.instagram.com/bradschoenfeldphd/ https://www.facebook.com/brad.schoenfeld.cscs https://twitter.com/BradSchoenfeld Thanks, please comment, like and subscribe! COACHING: https://revivestronger.com/online-coaching/ MEMBERSITE: https://revivestronger.com/team-revive-stronger/ WEBSITE: https://www.revivestronger.com MINI CUT MOVEMENT: https://revivestronger.com/mini-cut-movement/ FACEBOOK: http://www.facebook.com/revivestronger INSTAGRAM: http://www.instagram.com/revivestronger NEWSLETTER: https://bit.ly/2rRONG5 YOUTUBE: https://www.youtube.com/watch?v=XjWbbkgZg2Q __________________________________________________________________ If you want to support us via a donation, that's highly appreciated! Patreon • https://www.patreon.com/revivestronger Don't like Patreon, go to Paypal! • https://bit.ly/2XZloJ4 __________________________________________________________________ Our Ebooks! Ultimate Guide To Contest Prep Ebook: • https://revivestronger.com/product/the-ultimate-guide-to-contest-prep/ Primer Phase Ebook: • https://revivestronger.com/product/the-primer-phase/ __________________________________________________________________ Stay up to date with the latest research and educate yourself! MASS (Research Review): • https://goo.gl/c7FSJD RP+ Membership: • https://ob262.isrefer.com/go/plus/Steve90/ JPS Mentorship • https://jpseducation.mykajabi.com/a/13324/esJ8AZwy __________________________________________________________________ Books we recommend! Muscle & Strength Pyramids • https://goo.gl/S8s6tG RP Books • http://bit.ly/2vREaH0 RP + Members site • https://ob262.isrefer.com/go/plus/Steve90/ For more • http://revivestronger.com/library/ __________________________________________________________________ __________________________________________________________________ When you're interested in online coaching, please go visit our website and follow the application form: https://www.revivestronger.com/online-coaching/

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The Vitamin D Metabolite Ratio is Independent of Vitamin D Binding Protein Concentration

Play Episode Listen Later Jan 31, 2021 12:05

independent protein vitamin d concentration binding metabolite

Q+A: Using Metabolite Training, Pushing & Pulling Calories, And More...

Living Lean

Play Episode Listen Later Jan 1, 2021 22:54

Q&A! Today, we're diving into the ins & outs of metabolite training, when you should and shouldn't push or pull calories, and more.Click here now to apply for online coaching with our team.

training pulling calories metabolite

12. DR. ERIC HELMS: BODYBUILDING PERIODIZATION - DUP, Length of Massing, Metabolite Training, Macrocycles

Swole Radio

Play Episode Listen Later Nov 29, 2020 72:17

Dr. Eric Helms is a leading strength and hypertrophy expert. We spoke in depth about periodization for bodybuilders: Units of time in the training year DUP (daily undulating periodization) Mesocycle progression Progression across mesocycles: volume, frequency, rep ranges, exercise selection Metabolite blocks Length of massing phases Optimal body fat percentage for muscle growth Rates of gain Resensitization phases Frequency of fat loss phases Where he’s at in his season Find Dr. Helms at: https://3dmusclejourney.com/ IG: @helms3dmj ------------------------------- Follow me on social media: YOUTUBE: www.youtube.com/c/AskDrSwole INSTAGRAM: http://instagram.com/dr_swole FACEBOOK: https://www.facebook.com/bill.wong22/ TIKTOK: https://www.tiktok.com/@dr_swole/ ------------------------------- About me: I'm a medical doctor and natural men’s physique athlete based in Vancouver, Canada. I seek to share science-based perspectives to help people lose fat and gain muscle. These will reflect the current expert consensus in the scientific bodybuilding community. I‘m lifetime drug-free and have been training since 2012. ------------------------------- Disclaimers: Consider seeing a physician to assess your readiness before beginning any fitness program. Information presented here is to be applied intelligently in the individual context. I do not assume liability for any loss incurred by using information in this podcast.

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Public LC-Orbitrap-MSMS Spectral Library for Metabolite Identification

Play Episode Listen Later Nov 22, 2020

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2020.11.21.392266v1?rss=1 Authors: Phapale, P., Palmer, A., Gathungu, R. M., Kale, D., Brugger, B., Alexandrov, T. Abstract: Liquid chromatography-mass spectrometry(LC-MS)-based untargeted metabolomics studies require high-quality spectral libraries for reliable metabolite identification. We have constructed EMBL-MCF, an open LC-MS/MS spectral library that currently contains over 1600 fragmentation spectra from 435 authentic standards of endogenous metabolites and lipids. The unique features of the library are presence of chromatographic profiles acquired with different LC-MS methods and coverage of different adduct ions. The library covers many biologically important metabolites with some unique metabolites and lipids as compared to other public libraries. The EMBL-MCF spectral library is created and shared using an in-house developed web-application at https://curatr.mcf.embl.de/. The library is freely available online and also integrated with other mass spectral repositories. Copy rights belong to original authors. Visit the link for more info

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ZIKA virus effects on neuroprogenitors are exacerbated by the main pyriproxyfen metabolite via thyroid hormone signaling disruption

PaperPlayer biorxiv bioinformatics

Play Episode Listen Later Nov 3, 2020

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2020.11.03.366468v1?rss=1 Authors: Spirhanzlova, P., Sebillot, A., Vancamp, P., Gothie, J.-D., Le Mevel, S., Leemans, M., Wejaphikul, K., Meima, M., Mughal, B. B., Butruille, L., Roques, P., Remaud, S., Fini, J.-B., Demeneix, B. A. Abstract: North-Eastern Brazil saw intensive application of the insecticide pyriproxyfen (PPF) during the microcephaly outbreak caused by Zika virus (ZIKV). ZIKV requires the neural RNA-binding protein Musashi-1 to replicate. TH represses MSI1. Being a suspected TH disruptor, we hypothesized that co-exposure to the main metabolite of PPF, 4'-OH-PPF, would exacerbate ZIKV effects through increased MSI1 expression. This was tested using in vitro mouse neurospheres and an in vivo TH signaling reporter model, Xenopus laevis. TH signaling was decreased by 4'-OH-PPF in both models. In mouse-derived neurospheres the metabolite reduced neuroprogenitor proliferation as well as markers of neuronal differentiation. The results demonstrated that 4'-OH-PPF significantly induced MSI1 at both the mRNA and protein level, as well as Fasn mRNA. Other TH target genes were also significantly modified. Importantly, several key genes implicated in neuroprogenitor fate and commitment were not dysregulated by 4'-OH-PPF alone, but were in combination with ZIKV infection. These included the neuroprogenitor markers Nestin, Egfr, Gfap, Dlx2 and Dcx. Unexpectedly, 4'-OH-PPF decreased ZIKV replication, although only at the fourth and last day of incubation, and RNA copy numbers stayed within the same order of magnitude. However, intracellular RNA content of neuroprogenitors was significantly decreased in the combined presence of the PPF metabolite and ZIKV. We conclude that 4'-OH-PPF interferes with TH action in vivo and in vitro, inhibiting neuroprogenitor proliferation. In the presence of ZIKV, TH signaling pathways crucial for cortical development are significantly impacted. This provides another example of viral effects that are exacerbated by drug or pesticide use. Copy rights belong to original authors. Visit the link for more info

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Joint automatic metabolite identification and quantification of a set of 1H NMR spectra

Play Episode Listen Later Oct 9, 2020

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2020.10.08.331090v1?rss=1 Authors: Lefort, G., Liaubet, L., Marty-Gasset, N., Canlet, C., Vialaneix, N., Servien, R. Abstract: Metabolomics is a promising approach to characterize phenotypes or to identify biomarkers. It is also easily accessible through NMR, which can provide a comprehensive understanding of the metabolome of any living organisms. However, the analysis of 1H NMR spectrum remains difficult, mainly due to the different problems encountered to perform automatic identification and quantification of metabolites in a reproducible way. In addition, methods that perform automatic identification and quantification of metabolites often do it for one given complex mixture spectrum. Hence, when a set of complex mixture spectra coming from the same experiment has to be processed, the approach is simply repeated independently for every spectrum, despite their resemblance. Here, we present a new method that is the first to identify and quantify metabolites by integrating information coming from several complex spectra of the same experiment. The performances of this new method are then evaluated on both simulated and real datasets. The results show an improvement in the metabolite identification and in the accuracy of metabolite quantifications, especially when the concentration is low. This joint procedure is available in version 2.0 of ASICS package. Copy rights belong to original authors. Visit the link for more info

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Drunk CrossFit WOD (ft. Bryan Boorstein)

Total Fitheads

Play Episode Listen Later Sep 30, 2020 62:18

Bryan Boorstein is the coach that created Evolved Training Systems and Paragon Training. He's a CrossFit regional athlete, L2 trainer and has coached over 75 athletes to the CrossFit Games. He's evolved from functional fitness to physique and offers hybrid programs that includes strength, Olympic lifting, bodybuilding, gymnastics and more. We talk with him about everything from hormones to metabolite training to macros to reverse pyramid training and more. Also, he did Fran drunk.

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170: The Improvement Season - Were the Bros right all along?

Play Episode Listen Later Sep 21, 2020 63:45

This time we're talking about whether the bros have been right all along? Timestamps 00:00 Intro 03:45 Pumps 05:56 Metabolite training 10:46 Nutrition for Pascal & Steve 22:08 Sleep and its impact on overall wellbeing 26:22 Struggling with eating more food 33:48 Have the bros been right all along? 47:27 Thinking in a box 49:38 Broish mentality and tribalism 52:01 Staying true to yourself, innovators 56:39 Development of science Thanks, please comment, like and subscribe! COACHING: https://revivestronger.com/online-coaching/ MEMBERSITE: https://revivestronger.com/team-revive-stronger/ WEBSITE: https://www.revivestronger.com MINI CUT MOVEMENT: https://revivestronger.com/mini-cut-movement/ FACEBOOK: http://www.facebook.com/revivestronger INSTAGRAM: http://www.instagram.com/revivestronger NEWSLETTER: https://bit.ly/2rRONG5 YOUTUBE: https://www.youtube.com/watch?v=_SftQwB8CC4 __________________________________________________________________ If you want to support us via a donation, that's highly appreciated! Patreon • https://www.patreon.com/revivestronger Don't like Patreon, go to Paypal! • https://bit.ly/2XZloJ4 __________________________________________________________________ Our Ebooks! Ultimate Guide To Contest Prep Ebook: • https://revivestronger.com/product/the-ultimate-guide-to-contest-prep/ Primer Phase Ebook: • https://revivestronger.com/product/the-primer-phase/ __________________________________________________________________ Stay up to date with the latest research and educate yourself! MASS (Research Review): • https://goo.gl/c7FSJD RP+ Membership: • https://ob262.isrefer.com/go/plus/Steve90/ JPS Mentorship • https://jpseducation.mykajabi.com/a/13324/esJ8AZwy __________________________________________________________________ Books we recommend! Muscle & Strength Pyramids • https://goo.gl/S8s6tG RP Books • http://bit.ly/2vREaH0 RP + Members site • https://ob262.isrefer.com/go/plus/Steve90/ For more • http://revivestronger.com/library/ __________________________________________________________________ __________________________________________________________________ When you're interested in online coaching, please go visit our website and follow the application form: https://www.revivestronger.com/online-coaching/

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Modular metabolite assembly in C. elegans lysosome-related organelles

Play Episode Listen Later Aug 24, 2020

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2020.08.22.262956v1?rss=1 Authors: Le, H. H., Wrobel, C. J., Cohen, S. M., Yu, J., Park, H., Helf, M. J., Curtis, B. J., Rodrigues, P. R., Sternberg, P. W., Schroeder, F. C. Abstract: Signaling molecules derived from attachment of diverse primary metabolic building blocks to ascarosides play a central role in the life history of C. elegans and other nematodes; however, many aspects of their biogenesis remain unclear. Using comparative metabolomics, we show that lysosome-related organelles (LROs) are required for biosynthesis of most modular ascarosides as well as previously undescribed modular glucosides. Both modular glucosides and ascarosides are derived from highly selective assembly of moieties from nucleoside, amino acid, neurotransmitter, and lipid metabolism. We further show that cholinesterase (cest) homologs that localize to the LROs are required for assembly of both modular ascarosides and glucosides, mediating formation of ester and amide linkages between subsets of building blocks. Their specific biosynthesis suggests that modular glucosides, like ascarosides, serve dedicated signaling functions. Further exploration of LRO function and cest homologs in C. elegans and other animals may reveal additional new compound families and signaling paradigms. Copy rights belong to original authors. Visit the link for more info

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The malaria metabolite HMBPP does not trigger erythrocyte terpene production

Play Episode Listen Later Jul 30, 2020

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2020.07.29.225839v1?rss=1 Authors: Miller, J., Odom John, A. R. Abstract: Infection with malarial parasites renders hosts more mosquito attractive than their uninfected, healthy, counterparts. One volatile organic compound, -pinene, is associated with Plasmodium spp. infection in multiple studies and is a known mosquito attractant. However, how malarial infection results in elevated levels of host-associated -pinene remains unclear. One study suggests that erythrocyte exposure to the malarial metabolite, (E)-4-hydroxy-3-methyl-but-2-enyl pyrophosphate (HMBPP), results in increased levels of -pinene. Here, we establish that endogenous levels of -pinene are present in human erythrocytes, that these levels vary widely by erythrocyte donor, and that -pinene levels are not altered by HMBPP treatment. Copy rights belong to original authors. Visit the link for more info

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Development and validation of an HPLC method to quantify 2-Keto-3-deoxy-gluconate (KDG), a major metabolite in pectin and alginate degradation pathways

Personalized Medicine Podcast

Play Episode Listen Later Jul 25, 2020

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2020.07.24.220400v1?rss=1 Authors: Martis B, S., Droux, M., Deboudard, F., Nasser, W., Meyer, S., Reverchon, S. Abstract: A rapid and sensitive High Performance Liquid Chromatography (HPLC) method with UV and fluorescence detection is developed for routine analysis of 2-Keto-3-deoxy-gluconate (KDG), a catabolite product of pectin and alginate. These polysaccharides are both used for biofuel production and to generate high-value-added products. HPLC is performed, after derivatization of the carbonyl groups of the metabolite with O-phenylenediamine (OPD), using a linear gradient of trifluoroacetic acid and acetonitrile. Quantification is accomplished with an internal standard method. The gradient is optimized to distinguish KDG from its close structural analogues such as 5-keto-4-deoxyuronate (DKI) and 2,5-diketo-3-deoxygluconate (DKII). The proposed method is simple, highly sensitive and accurate for time course analysis of pectin or alginate degradation. Copy rights belong to original authors. Visit the link for more info

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Ep#015: Enabling Personalized Medicine through Biomarkers of Response with Dr. Liz O'Day

Play Episode Listen Later Jul 1, 2020 53:08

In episode 15, our host Oleksandr Yagensky sat down with Dr. Liz O’Day, the founder and CEO of Olaris, a biotech company that enables patients to pursue most effective personalized treatments. Liz unravels how small molecule metabolites along with machine learning can be utilized to evaluate patient-specific responses to therapeutics. Together with Olaris, Liz is shaping the future of personalized medicine. Just two weeks ago, she was elected to the Board of Directors at Personalized Medicine Coalition in recognition of her contribution to the field.Together with Liz, we discussed: ◦ The startup story of Olaris ◦ Biomarkers of Response (BORs) ◦ The future of biomarker panels and multi-omics ◦ Metabolite based biomarker profiling ◦ Analytical platforms used at Olaris ◦ How Olaris leverages machine-learning to find patient-specific metabolite patterns ◦ Application cases for Olaris technology in cancer treatment ◦ Three part aim of Olaris ◦ Bringing the stakeholders in healthcare together ◦ Role of science in becoming a successful entrepreneur ◦ Common challenges faced by biotech start-ups ◦ The future of personalized medicineGet in touch with Liz: ◦ LinkedIn: @Liz O'Day ◦ Twitter: @Lizzard0126 ◦ Facebook: https://www.facebook.com/olarisBoR/ ◦ Instagram: https://www.instagram.com/lizzard0126/ ◦ Web: https://www.olarisbor.com/Make sure to download the full show notes with our guest's bio, links to their most notable work, and our recommendations for further reads on the topic of the episode at pmedcast.com

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Laktat

Prestasjonsprat

Play Episode Listen Later Jun 24, 2020 48:55

Laktat er noe vi snakker om, måler og forsøker å benytte i daglig treningsarbeid, særlig når det gjelder såkalt terskeltrening.Blant trenere, mosjonister og idrettsutøvere omtales ofte konseptene rundt laktat-terskel og anaerob terskel litt feil, sannsynligvis fordi fysiologien er mer kompleks enn vi klarer å ta innover oss. I tillegg har laktat, eller "melkesyre", har fått et ufortjent dårlig rykte som vi vil til livs! Lær om historien bak laktat og anaerob terskel og om noen av laktat sine viktige og spennende funksjoner i kroppen. Følg oss i sosiale medier på @prestasjonsprat Referanse artikler: Proia, P.; Di Liegro, C.M.; Schiera, G.; Fricano, A.; Di Liegro, I. Lactate as a Metabolite and a Regulator in the Central Nervous System. Int. J. Mol. Sci. 2016, 17, 1450 https://www.mdpi.com/1422-0067/17/9/1450 Barros, L. F. (2013). Metabolic signaling by lactate in the brain. Trends in Neurosciences, 36(7), 396-404https://www.sciencedirect.com/science/article/pii/S0166223613000635 Ferguson, B.S., Rogatzki, M.J., Goodwin, M.L. et al. Lactate metabolism: historical context, prior misinterpretations, and current understanding. Eur J Appl Physiol 118, 691–728 (2018). https://doi.org/10.1007/s00421-017-3795-6 https://dx.doi.org/10.1007/s00421-017-3795-6 See acast.com/privacy for privacy and opt-out information.

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NMR Analysis of the Correlation of Metabolic Changes in Blood and Cerebrospinal Fluid in Alzheimer Model Male and Female Mice

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Play Episode Listen Later Jun 1, 2020

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2020.05.26.116525v1?rss=1 Authors: Stojanovic, F., Taktek, M., Khieu, N. H., Huang, J., Jiang, S., Rennie, K., Chakravarthy, B., Costain, W. J., Cuperlovic-Culf, M. Abstract: The development of effective therapies as well as early, molecular diagnosis of Alzheimers disease is impeded by the lack of understanding of the underlying pathological mechanisms. Metabolomics studies of body fluids as well as brain tissues have shown major changes in metabolic profiles of Alzheimers patients. However, with analysis performed at the late stages of the disease it is not possible to distinguish causes and consequence. The mouse model APP/PS1 expresses a mutant amyloid precursor protein resulting in early Amyloid {beta} (A{beta}) accumulation as well as many resulting physiological changes including changes in metabolic profile and metabolism. Analysis of metabolic profile of cerebrospinal fluid (CSF) and blood of APP/PS1 mouse model can provide information about metabolic changes in these body fluids caused by A{beta} accumulation. Using our novel method for analysis of correlation and mathematical ranking of significant correlations between metabolites in CSF and blood, we have explored changes in metabolite correlation and connectedness in APP/PS1 and wild type mice. Metabolites concentration and correlation changes in CSF, blood and across the blood brain barrier determined in this work are affected by the production of amyloid plaque. Metabolite changes observed in the APP/PS1 mouse model are the response to the mutation causing plaque formation, not the cause for the plaque suggesting that they are less relevant in the context of early treatment and prevention then the metabolic changes observed only in humans. Copy rights belong to original authors. Visit the link for more info

Whole-Brain High-Resolution Metabolite Mapping with 3D Compressed-Sensing-SENSE-LowRank 1H FID-MRSI

Play Episode Listen Later May 20, 2020

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2020.05.18.101618v1?rss=1 Authors: Klauser, A., Klauser, P., Grouiller, F., Courvoisier, S., Lazeyras, F. Abstract: There is a growing interest of the neuroscience community to map the distribution of brain metabolites in vivo. Magnetic resonance spectroscopy imaging (MRSI) is often limited by either a poor spatial resolution and/or a long acquisition time which severely limits its applications for clinical or research purposes. We developed a novel acquisition-reconstruction technique combining fast 1H-FID-MRSI sequence accelerated by random k-space undersampling and a low-rank and total-generalized variation (TGV) constrained model. This framework was applied to the brain of four healthy volunteers. Following 20 min acquisition, reconstruction and quantification, the resulting metabolic maps with a 5mm isotropic resolution reflected the detailed neurochemical composition of all brain regions and revealed part of the underlying brain anatomy. Contrasts and features from the 3D metabolite distributions were in agreement with the literature and consistent across the four subjects. The successful combination of the 3D 1H-FID-MRSI with a constrained reconstruction enables the detailed mapping of metabolite concentrations at high-resolution in the whole brain and with an acquisition time that is compatible with clinical or research settings. Copy rights belong to original authors. Visit the link for more info

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Gut microbial metabolite p-Cresol promotes autistic-like behaviors in mice through remodeling of the microbiota

cross designed reacts urine detect metabolite

Play Episode Listen Later May 19, 2020

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2020.05.18.101147v1?rss=1 Authors: Bermudez-Martin, P., Becker, J. J., Fernandez, S. P., Costa-Campos, R., Barbosa, S., Martinez Gili, L., Myridakis, A., Dumas, M. E., Bruneau, A., Cherbuy, C., Langella, P., Chabry, J., Barik, J., Le Merrer, J., Glaichenhaus, N., Davidovic, L. Abstract: Background: Perturbations of the microbiota-gut-brain axis have been identified in autism spectrum disorders (ASD), suggesting that the microbiota could be involved in the development or maintenance of abnormal social and stereotyped behaviors in ASD patients. Yet, the underlying mediators and mechanisms remain unclear. We hypothesized that microbial metabolites produced by the gut microbiota contribute to behavioral deficits in ASD. We focused on p-Cresol, a microbial metabolite previously described as abnormally elevated in ASD patients. Methods: Wild-type mice were chronically treated with p-Cresol in drinking water to mimic intestinal exposure. We combined behavioral phenotyping, electrophysiology, microbiota 16S sequencing and fecal microbiota transplantations to decipher the consequences of p-Cresol exposure. Results: We showed that p-Cresol selectively induced behavioral alterations reminiscent of ASD core symptoms: social behavior deficits, stereotypies and perseverative behaviors, but no changes in anxiety, locomotion or cognition. We further showed that p-Cresol decreases the activity of dopamine neurons in the ventral tegmental area (VTA), a key brain region for social reward processing. In addition, we reveal that p-Cresol remodels the intestinal microbiome, impacting specific bacterial taxa associated with social behavior deficits and stereotypies. We further demonstrated that social behavior deficits are transferred to control mice after transplantation of microbiota from p-Cresol-treated mice. Finally, both social interactions and VTA dopamine neurons activity were normalized in p-Cresol treated mice after transplant of microbiota from control mice. Conclusions: Our study suggests that the microbial metabolite p-Cresol could be involved in the development of autistic behaviors through remodeling of the gut microbiota. Copy rights belong to original authors. Visit the link for more info

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Cannabinol (CBN) Cross-Reacts with Two Urine Immunoassays Designed to Detect Tetrahydrocannabinol (THC) Metabolite

JALM Talk Podcast

Play Episode Listen Later May 11, 2020 8:40

Dr Amy Beckley | Ovulation & Progesterone Metabolite: How they may play a role in your Fertility

Fempower Health

Play Episode Listen Later Apr 7, 2020 49:00

Hear an interview with Proov inventor and founder, Amy Galliher-Beckley, who created an at-home PdG, or progesterone metabolite, test to confirm ovulation. This is a completely innovative and novel diagnostic process for measuring PdG at home. We will learn about:The company and their product, Proov, Uncover why Amy created this test,The important role PdG levels plays in fertility, and The challenges women face in seeking help given the complex dynamics of our healthcare system. Fempower Health has developed a survey that leverages women's desire to help and advise other women based on their own experience and learnings from conditions like infertility, PCOS, endometriosis, and thyroid conditions. Go here to take survey and receive product discount as a thank you.Follow on Instagram and Facebook!#fempowerhealth #femcast NOTE: Fempower Health may receive a nominal fee for products purchased. The purpose of the podcast, however, is for education purposes only. Always go to a medical professional of your choosing.Support the show (https://www.patreon.com/fempowerhealth)

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Long-term Changes in Gut Microbial Metabolite Trimethylamine N-Oxide and Coronary Heart Disease Risk

JACC Podcast

Play Episode Listen Later Feb 18, 2020 11:33

Commentary by Dr. Valentin Fuster

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Using Metabolite Testing to Heal Your Gut

Precisione: The Healthcast

Play Episode Listen Later Jan 24, 2020 40:17

Erika Ebbel Angle received her Ph.D. in Biochemistry in 2012 from Boston University School of Medicine. She holds a B.S. from the Massachusetts Institute of Technology. In 2002 Erika founded Science from Scientists, an award-winning National nonprofit focused on improving Science, Technology, Engineering and Math (STEM) attitudes and aptitudes for children aged 9-13. She is also the co-founder and Director of Education for Robots In Service of the Environment (RSE), a non-profit organization whose mission is to apply robotic solutions to help solve environmental challenges. She is a member of the MIT visiting committee for the Dean of Undergraduate Education and is an Advisory Board member for the Ron Burton Training Village. She is also a STEM speaker for the U.S. Speaker Program at the U.S. Department of State. In 2018 she was awarded the Young Alumni award by Boston University. In 2017 she was the recipient of the Distinguished Alumni Award from Boston University School of Medicine Division of Graduate Medical Sciences. In 2014 the Boston Business Journal selected her as one of the 40 Under 40 business and civic leaders who are making a major impact in their respective fields in the Boston area. Her accomplishments have also been recognized by the Greater Boston Chamber of Commerce’s Pinnacle Awards for Emerging Executive. In 2013 the Boston Chamber of Commerce selected her as one of the Ten Outstanding Young Leaders in Boston. Selected by L’Oreal Paris as a 2007 Woman of Worth for her work with Science from Scientists, Erika has been featured on Lifetime TV and Nova Science Now on The Secret Lives of Scientists and Engineers. She is the host of The Dr. Erika Show, an educational science TV show for children. Erika served as a Commissioner for the MA Commission on the Status of Women. Erika was Miss Massachusetts 2004 in the Miss America Scholarship program. Outside of work, Erika enjoys SCUBA diving, skiing and, a graduate of the San Francisco Conservatory of Music, continues her lifelong passion for classical piano. She is married to Colin Angle, CEO of iRobot. What you will learn from this episode: 1) How a simple blood test can give you insight into your gut health. 2) What is the metabolome and why it is important? 3) Are stool tests good enough to teach us about our gut health? 4) How you can improve your fitness performance but learning about your gut health 5) What interventions you can make to improve your health and longevity How to learn more about our guest: Full Bio: https://ixcela.com/team-bios/erika-angle-phd.html Connect with Erika ● Blog: Erika Ebbel Angle ● LinkedIn: Erika Ebbel Angle ● Twitter: @DoctorErika A special thank you: As a special thank you for listening to this episode, Ixcela has been so kind as to give us a discount code which you can use to get a 10% discount. Go to ixcela.com and use the code “IxcelaWellness10” to check it out! Please enjoy, share, rate and review our podcast and help us bring the message about precision health care to the world!

Neil Rogers Show (May 26, 1999)

Neil Rogers Show

Play Episode Listen Later Oct 9, 2019 161:43

Metabolite, more OJ, YT embed issue

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Best Cutting Steroid Cycle - The Steroids Podcast Episode 17 Special 90 Minute Episode

Steroids Podcast

Play Episode Listen Later Sep 21, 2019 84:55

Ask Your Questions For the Next Episode at - https://steroidspodcast.com 0:00 Ultimate Guide to Roids 4:45 My Experience Writing the Best Bodybuilding Book Ever Released 7:50 Testosterone Cypionate 300mg Equipoise 300mg Primobolan 500mg per week cycle 11:25 Powers of Primbolan 13:13 How to tell Real vs. Fake Bayer Primobolan 16:16 Primobolan Nitrogen Retention Effects 18:00 Trenbolone Side Effects 18:56 What it means to “Go Flat” in Bodybuilding 20:11 Primobolan Side Effects 20:55 Trenbolone vs Primobolan 22:30 Realistic Dosages of Anabolic Steroids for Bodybuilding 24:42 How Long Does It Take Anadrol to Kick In 26:41 What is EPO and Blood Doping in Cycling and Endurance Sports 28:27 Best Pre workout Drink for Cardio 30:16 How Come Dick is Smaller on Tren? 32:05 Trenbolone Jack of All Spades Hormone in the Penis 33:10 Tren Shrinks Your Balls More Than Any Other Steroid Some Guys Lose Their Nuts and Cannot Find Them Up Inside Their Abdomen 34:00 Trenbolone “Empty Sac” Side Effect 34:50 How to Spot a Tren Abuser 35:10 Trenbolone Night Sweats 36:05 Primobolan Minimum Effective Dosage 36:38 High Reps vs Low Reps For Dense Muscle Growth with Steroids 38:20 What it Means to Optimize your Training Diet and Steroids 39:37 Indicators of Progress in the Gym 41:19 Middle Age TRT Guy runs two cycles of Equipoise and Primobolan 16 weeks per year 43:18 What is a Metabolite of a Steroid 44:45 Methyl Trenbolone Methyl Deca Oral Stack 47:05 Methyltrienolone in Scientific Experiments 48:10 Methy Tren Effects and Stereotypes 49:49 First Steroid Cycle 250mg Testosterone Enanthate 10 Weeks with HCG for PCT 51:00 How to Prevent Permanent Gyno “Bitch Tits” 53:20 Best PCT for Steroid Cycle 56:10 Bad Reaction and Swelling from Synthetic OIls in UGL Gear 56:30 What is a UGL 58:20 How to Prevent Infection from Steroids Injection 59:52 How Long Does Tren Ace take to Kick In 1:02:00 Best Fat Burning Steroids 1:03:40 HGH Permanent Gains and Side Effects 1:05:18 Reality of HGH Gains and Going off Steroid Cycle 1:05:40 Growth Hormone Side Effects 1:06:47 Anavar Only Cycle Libido and Penis 1:09:03 500mg Sustanon 300 Propionate 300 Trenbolone 300 Masteron 50mg per day Dbol Cycle 1:10:55 Best Way to Prevent Estrogen Side Effects 1:11:47 Beginner Cutting Cycle 1:15:00 Masteron Libido Effect 1:17:30 20mg Dianabol 25mg Anadrol Methyl Testosterone 5mg Tablet Explanation 1:19:40 Aromasin Exemestane Side Effects 1:20:10 What “Half Life” of Steroid Means This Podcast is for entertainment and conversational purposes only. This author does not support the use of illegal performance enhancing drugs. If any substances mentioned in this video are illegal in your country do not use them. Consult a doctor before beginning any exercise or supplement routine. Do not take anything mentioned in this video as advice. It is simply conversation, not advice.

MAX MPS RADIO #73: Dr. Mike Israetel - Overload Training Modalities Ep. 2 - Metabolite Techniques

MAX MPS RADIO

Play Episode Listen Later Sep 9, 2019 86:52

Dr. Mike Israetel, Ph.D. in Sport Physiology and Co-Founder of Renaissance Periodization is back on the show. In this episode Mike dives deeper into the overload training modalities including higher rep sets and metabolite techniques like giant sets, myo reps, drop sets and BFR training. These topics will be covered in depth in his upcoming book “Scientific Principles of Hypertrophy Training” which will be released next year. 01:23 What exactly is metabolic stress? Where would you place its importance in contrast to mechanical tension in hypertrophy training? 5:50 Giant Sets 16:07 Myo Reps 42:55 Drop Sets 46:24 How do you program Drop Sets? 50:27 Is there a bottom range intensity for drop sets? 52:45 How do you program relative intensities for metabolite techniques? 01:01:44 Supersets 01:02:20 Compound alternating supersets 01:14:12 Blood Flow Restriction Training (BFR) 01:17:32 Do you think there is a specific advantage to BFR vs. just normal low load training? 01:18:49 How do you track sets for metabolite techniques? 01:23:24 What Mike is up to in the next few months Mikes Facebook: https://www.facebook.com/michael.isra... Mikes Instagram: https://www.instagram.com/rpdrmike/ Renaissance Periodization: https://renaissanceperiodization.com Renaissance Periodization Instagram: https://www.instagram.com/rpstrength/ RP+: https://renaissanceperiodization.com/... How much should I train: https://renaissanceperiodization.com/...... ______________________________________________ Thanks for listening/watching! If you liked the podcast please like, comment, share and subscribe to the channel! This podcast is also available on all other streaming-platforms: Itunes: https://itunes.apple.com/de/podcast/m... Soundcloud: https://soundcloud.com/janfrisse/ Spotify: https://open.spotify.com/show/1OmzAJu... ______________________________________________ Webseite/Website: http://janfrisse.de Online Personal Training: http://janfrisse.de/online-personal-t... Kostenloses Beratungsgespräch/Free Skype Consulting: http://janfrisse.de/kontakt Instagram: https://www.instagram.com/janfrisse Youtube: https://www.youtube.com/channel/UCX_O... Facebook: https://www.facebook.com/janfrissept E-Mail: coaching@janfrisse.com ______________________________________________ For my English audience: My name is Jan Frisse a full time online coach located in Essen, Germany and I am an intern with Dr. Mike Israetel. You are more than welcome to join! I will regularly release English content with the top scientists, experts, coaches and athletes in the field of the evidence-based fitness industry. Just keep in mind that this is a bilingual podcast, so there will be German content since my main target audience is Germany. I’m still absolutely happy to have you on here as well so you’re more than welcome to stay! "MAX MPS RADIO" ist ein bilingualer Podcast mit evidenz-basierten Content zum Thema Bodybuilding, Fitness, Ernährung und den Wissenschaften dahinter! Jan interviewt die Top-Experten im Feld der evidenzbasierten Fitness-Industrie und produziert Co-Host Serien mit anderen Coaches und Athleten!

Metabolite Markers -- Heard in the Halls: Voices From the 2019 American Society of Clinical Oncology Annual Meeting

Breastcancer.org Podcast

Play Episode Listen Later Jun 5, 2019 3:35

voices heard american society halls annual meetings markers clinical oncology metabolite

Validation of a Metabolite Panel for a More Accurate Estimation of Glomerular Filtration Rate Using Quantitative LC-MS/MS

Clinical Chemistry Podcast

Play Episode Listen Later Mar 26, 2019 8:33

Glomerular filtration rate, or GFR, is generally accepted as the best overall index of kidney function, and a decrease in GFR has important implications for prognosis in patient management. GFR is most commonly estimated by calculation, using the serum concentration of an endogenous filtration marker such as creatinine and demographic variables such as age, sex, and race. In the March 2019 issue of Clinical Chemistry, a paper investigated the possibility of developing a more accurate estimate of GFR, using a panel of metabolites measured by quantitative liquid chromatography, tandem mass spectrometry without creatinine, cystatin C, or demographic variables.

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#10 Schlafmittel - 2 Mio. Abhängige in Deutschland

Gesünder mit praktischer Medizin

Play Episode Listen Later Jan 27, 2019 33:39

In dieser 3. Podcast-Folge zum Thema Schlaf spricht Prof. Dr. med. Harald Schmidt über Schlafmittel. Da das Thema doch recht umfangreich ist geht es heute um die chemischen Substanzen und das große Problem von 2 Millionen Schlafmittelabhängigen in Deutschland In einer weiteren Folge um die Pflanzlichen. Der neueste Stand der Medizin, Evidenz-basiert und direkt umsetzbare praktische Impulse warten auf Sie. // Vorbemerkungen // Wann? // Ich sag’s gleich, wie auch schon in der letzten Folge zur Schlafhygiene, ich bin kein Freund von Schlafmitteln. Ausnahmen sind zwei nachvollziehbare Gründe: Jetlag ein emotional stark bewegendes/belastenden Erlebnis. // Wann nicht // Schlaftabletten zu verwenden, um z.B. trotz der Nervosität vor einer Prüfung schlafen zu können, ist hingegen keine gute Idee. Fast alle Schlafmittel können bis in den Tag hineinwirken und die körperliche und geistige Leistungsfähigkeit stark einschränken. // Abhängigkeit // Schlafmittel sind in aller Regel nicht für die Selbstbehandlung geeignet. Das gilt vor allem für verschreibungspflichtige Schlafmittel wie Benzodiazepine, die schnell eine Abhängigkeit verursachen können. Sie können dann nicht mehr ohne Schlafmittel schlafen. Die Schlafmittel-Abhängigkeit wird zur Ursache Ihrer Schlafstörung oder Sie haben nun zwei Schlafstörungen. Aber auch nicht verschreibungspflichtige, aber apothekenpflichtige chemische sowie pflanzliche Schlafmittel können zumindest psychisch abhängig machen. Etwa die Hälfte der Benutzer von Schlafmitteln entwickelt nach Angaben der Deutschen Hauptstelle für Suchtgefahren eine Schlafmittel-Abhängigkeit. Das sind allein in Deutschland 400.000 Schlafmittel-Süchtige jedes Jahr. Insgesamt gelten etwa 2 Millionen Menschen als abhängig von Schlafmitteln. Etwa die Hälfte dieser Menschen konsumiert die häufig verschriebenen Benzodiazepine. Als besonders anfällig für Medikamentensucht und Schlafmittelmissbrauch gelten Frauen. Zahlreiche Studien legen nahe, dass Schlafmittel die Lebenserwartung deutlich verkürzen können. // Nur kurzfristig! // Schlafmittel sind nicht für den langfristigen Einsatz gedacht. Ohne ärztliche Weisung sollten Sie Schlafmittel nie länger als eine Woche anwenden. Sollte Ihr Arzt immer wieder ohne Nachfragen Schlafmittel-Rezepte ohne besondere Diagnose ausstellen, wenden Sie sich am besten an einen anderen Arzt, um ihre Schlafstörungen besser zu behandeln und wenden Sie die Tipps meiner vorherigen Podcast-Folge “Endlich besser schlafen” an. // Das ideale Schlafmittel... // … gibt es nicht. Es müsste einen Schlaf bewirken, der sich vom natürlichen Schlaf in nichts unterscheidet. Alle heute verwendeten Mittel verändern jedoch die Schlafstadien und die messbaren Hirnströme. GABAA // Die meisten Schlafmittel wirken über einen Ionenkanal der durch den Neurotransmitter γ-Aminobuttersäure reguliert wird. γ-Aminobuttersäure = GABA und hiervon der Typ A also GABAA. Schlafmittel binden entweder an GABA Bindungsstelle oder an eine eigene. Dadurch kommt es zum Einstrom negativer Cl Ionen. Da Nervenzellen durch den Einstrom positiver Ionen aktiviert werden, hemmt dies also die Nervenzellen. GABAA. Ist dabei der wichtigste hemmende Rezeptor im zentralen Nervensystem. Dadurch wird zwar Schlaf induziert, aber auf Kosten des erholsamen REM- und Tiefschlafs. Nach Absetzen vieler Schlafmittel gibt es zunächst einen überschießenden REM-Schlaf während sich der Tiefschlaf nur langsam erholt. Übrigens, auch Alkohol bindet an diesen GABAA-Rezeptor wodurch sich dessen sedierende Wirkung erklärt; aber ich erwähnte unter Schlafstörungen dass Alkohol ein denkbar ungeeigneter Schlafförderer ist, da dessen Metabolite während der Nacht den Schlaf stören und Durchschlafen verhindern. // Verschreibungspflichtige chemische Arzneimittel: // Barbiturate // Nicht mehr als Schlafmittel,...

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Association between metabolite composition and metabolic risk across adolescence

Pediatric Research Podcast

Play Episode Listen Later Jan 25, 2019 10:02

Metabolomics has the potential to identify specific targets for primary prevention of metabolic disease. Studies in adults have shown that lean vs obese people show distinct differences in their metabolite composition, sometimes preceding the development of established risk factors associated with metabolic disease. The literature in paediatric populations, however is scant. In this episode, we speak to Prof. Wei Perng, who during her time at the University of Michigan School of Public Health, examined the associations between metabolite composition and metabolic risk across adolescence. See acast.com/privacy for privacy and opt-out information.

university risk prof studies public health composition adolescence metabolic michigan school metabolomics metabolite

The End of ENDoxifen Metabolite and CYP2D6 Testing in Tamoxifen-Treated Women?

Journal of Clinical Oncology (JCO) Podcast

Play Episode Listen Later Jan 24, 2019 6:05

This JCO Podcast provides observations and commentary on the JCO article “Tamoxifen pharmacogenetics and metabolism: Results from the prospective CYPTAM study” by Sanchez-Spitman et al. My name is Vered Stearns, and I am a Professor of Oncology and Co-Director of the Breast and Ovarian Cancer Program at the Kimmel Cancer Center at Johns Hopkins in Baltimore, Maryland. My oncologic specialty is medical oncology. In the paper that accompanies this podcast, the authors report results of a prospective clinical study designated CYPTAM, which was designed to correlate endoxifen serum concentrations and outcomes of women prescribed adjuvant tamoxifen. The investigators enrolled 667 women with breast cancer who were initiating tamoxifen or who have been on tamoxifen for fewer than 12 months. The investigators obtained blood samples for CYP2D6 genotyping using the Amplichip CYP450 Test, and measured steady state concentrations of endoxifen with a high-performance liquid chromatography-tandem mass spectrometry. Co-primary endpoints included association of recurrence-free survival with endoxifen concentrations and with CYP2D6 genotypes. The patients were censored at the time of tamoxifen-discontinuation in case of a transition to an aromatase inhibitor. Several additional endpoints included disease-free survival, complete relapse-free survival, complete disease-free survival and overall survival. The statistical analysis plan was designed as a gate-keeper analysis for the co-primary objectives. Only if an association was found with a p-value below 0.05, were the remaining objectives considered. The authors were not able to demonstrate an association between endoxifen concentrations and recurrence-free survival on tamoxifen. They also were not able to demonstrate an association either when exploring endoxifen concentrations in quartiles or when considering other thresholds. Likewise, there was no association between CYP2D6 genotypes and recurrence-free survival. Almost two decades ago, researchers recognized that the absence or inhibition of the CYP2D6 enzyme is associated with very low concentrations of endoxifen, a potent and abundant anti-estrogen metabolite of tamoxifen. Whether low concentrations of endoxifen predict an inferior survival outcome has not been definitively determined. Multiple retrospective and small prospective studies evaluated CYP2D6 genotypes and survival outcomes and have provided mixed evidence. Clinicians have, therefore, wondered whether CYP2D6 genotype testing or endoxifen monitoring will assist in treatment recommendations. The CYPTAM investigators attempted to prospectively correlate endoxifen serum concentrations and outcomes for women taking tamoxifen. The CYPTAM study is associated with several limitations, and, therefore, it does not provide a definitive answer to the controversy. For example, women were enrolled in the study either before starting tamoxifen or up to 12 months after initiation of the drug. This strategy could have led to incomplete baseline data and to the exclusion of individuals with early recurrences. In addition, about two-thirds of study participants transitioned to aromatase inhibitors following a short course of tamoxifen. The sequential administration of tamoxifen and aromatase inhibitors is superior to tamoxifen alone. Some of the patients who transitioned to aromatase inhibitors could have suffered a recurrence had they have stayed on tamoxifen alone. Furthermore, the authors did not have information regarding concomitant CYP2D6 inhibitor use. CYP2D6 inhibitors are commonly co-administered with tamoxifen and can contribute to misclassification of the CYP2D6 phenotype. The challenge moving forward is that single agent adjuvant tamoxifen is rarely used. Most postmenopausal women with hormone receptor-positive breast cancer are recommended an aromatase inhibitor instead of, or in sequence with, tamoxifen. Premenopausal women with high risk hormone receptor-positive tumors are recommended ovarian suppression with tamoxifen or an aromatase inhibitor. Women prescribed tamoxifen alone are usually at extremely low risk of recurrence, and a prospective study in this group of women will require a large number of participants to demonstrate differences between phenotypes. Given current practice, it may not be feasible to fully determine the role of endoxifen concentrations and CYP2D6 genotypes as predictors of tamoxifen efficacy. Retrospective analyses that used samples obtained through large prospective studies, such as the Arimidex, Tamoxifen, Alone or in Combination and the Breast International Group 1-98, failed to demonstrate an association between CYP2D6 phenotypes and survival outcomes. Taken together, the data at present are insufficient to recommend CYP2D6 testing or analysis of metabolic profile in women for whom tamoxifen is considered. Indeed, clinical guidelines from the American Society of Clinical Oncology and the National Comprehensive Cancer Network do not recommend CYP2D6 genotyping. Moving forward, prospective studies of altered metabolism due to single nucleotide polymorphism, or administration of inhibitors, should be considered in clinical trials of standard and novel agents as these can lead to differences in drug efficacy and toxicity. This concludes this JCO Podcast. Thank you for listening.

Ep.35 - Jon Jones & The Long Term Metabolite for Turinabol

Anti-Doping Science Podcast

Play Episode Listen Later Dec 27, 2018 54:44

Earlier this week it was announced that Jon Jones’s urine produced an Adverse Analytical Finding (AAF) for a long-term metabolite for the Anabolic Androgenic Steroid (AAS) Chlorodehydromethyltestosterone (DHCMT) – which is known by it’s trade name Oral-Turinabol. This is the 2nd time this long-term metabolite has been discovered inside Jon Jones urine. This podcast covers a detailed discussion on Jon Jones’s previous anti-doping rule violation for Clomiphene, his 2nd antidoping rule violation for the M3 metabolite and a discussion of the reappearance of this metabolite in his urine. I also discuss the research that has been conducted so far on the long-term metabolite (M3 metabolite) of Turinabol. Links to all reference material is below: Article on Jon Jones Fighting History: https://mmajunkie.com/2018/12/ufc-232-preview-jon-jones-career-statistics-facts Article on Atypical Finding announced in December 2018: https://mmajunkie.com/2018/12/jon-jones-drug-test-atypical-result-ufc-232-moved-to-los-angeles-gustafsson-dana-white Article on Jones’s 1st AAF for Clomiphene: http://www.espn.com/mma/story/_/id/25608180/jon-jones-long-winding-road-ufc-232 Article on Clomiphene https://anabolic.org/clomid-clomiphene-citrate/ USADA High Risk List of Supplements & Supplement 411: https://www.usada.org/substances/supplement-411/ USADA Arbitration Report on 2nd AAF for M3 Metabolite for Turinabol: https://ufc.usada.org/wp-content/uploads/Final-Redacted-Award-Jones-and-USADA.pdf Discovery of the M3 metabolite in 2012 by Sobolevsky & Rodchenkov: https://www.ncbi.nlm.nih.gov/pubmed/22142641 Critique of Sobolevsky & Rodchenkov’s research: https://www.researchgate.net/publication/305489931_Novel_metabolites_of_turinabol_WADA_moving_toward_illusions Synthesis of the M3 metabolite in 2018 (also discussing the extension in detection from the long term metabolite): https://pubs.rsc.org/en/content/articlelanding/2018/ob/c8ob00122g#!divAbstract Confirmation of the M3 metabolite in 2018: https://www.ncbi.nlm.nih.gov/pubmed/29570240

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084: The improvement season - Metabolite Frequency

coaching operation frequency strength training metabolite

Play Episode Listen Later Dec 18, 2018 61:43

The Ultimate Coach Seminar 2019 http://revivestronger.com/the-ultimate-coach-seminar/ This time we're talking about heart health, living life, things such as scars and tattoos for competing Timestamps: 35:21 Operation scars detrimental? 39:50 Metabolite technique frequency 47:45 how to gauge when to make adjustments Thanks, please comment, like and subscribe! COACHING: http://revivestronger.com/online-coaching/ WEBSITE: http://www.revivestronger.com FACEBOOK: http://www.facebook.com/revivestronger TWITTER: http://www.twitter.com/revivestronger INSTAGRAM: http://www.instagram.com/revivestronger MYFITNESSPAL: http://www.myfitnesspal.com/food/diary/snhall1990 YOUTUBE: https://www.youtube.com/watch?v=7GTnAOzvPV4 __ Stay up to date with the latest research! MASS (Research Review): • https://goo.gl/c7FSJD RP+ Membership: • https://ob262.isrefer.com/go/plus/Steve90/ __ Books we recommend! RP Books • http://bit.ly/2vREaH0 Scientific Principles of Strength Training • http://bit.ly/2w3th4D Renaissance Periodization Diet Ebook • http://bit.ly/2wGuuMU Understanding Healthy Eating • http://bit.ly/2uAxFZ8 RP + Members site • https://ob262.isrefer.com/go/plus/Steve90/ __ Recommended supplements: Denovo Nutrition (use code STEVE) • https://denovosupps.com?aff=6 __ When you're interested in online coaching, please go visit our website and follow the application form: http://www.revivestronger.com/online-coaching/

#31 - Navdeep Chandel, Ph.D.: metabolism, mitochondria, and metformin in health and disease

The Peter Attia Drive

Play Episode Listen Later Dec 3, 2018 142:24

In this episode, Nav Chandel, a professor of medicine and cell and molecular biology at Northwestern University, discusses the role of mitochondria and metabolism in health and disease. Nav also provides insights into the mitochondria as signaling organelles, antioxidants, and metformin’s multifaceted effects on human health, among many topics related to well-being. We discuss: What got Nav interested in mitochondria [5:00]; Reactive oxygen species (ROS) [16:00]; Antioxidants: helpful or harmful? [20:00]; Mitochondria as signaling organelles [22:00]; Hydrogen peroxide (H2O2) [25:00]; Mitochondrial DNA [28:00]; Mitochondria and aging [45:00]; Metformin [52:45]; Metformin and the gut microbiome [54:00]; Metformin as complex I inhibitor and the importance of the NADH/NAD ratio [1:01:00]; Anticancer benefits of metformin [1:07:45]; Mitochondrial function is necessary for tumorigenesis [1:15:00]; Are somatic mutations the result of mitochondrial dysfunction? [1:31:30]; Vitamins and antioxidants [1:37:00]; Targeting inflammation in disease [1:43:00]; NAD precursors [1:45:45]; MitoQ [1:52:00]; Metabolite toxicity [1:56:30]; Cortisol and healthy aging [2:02:00]; Nav turns the tables and asks Peter how he deals with the “So what should I eat?” question during social encounters [2:09:00]; and More. Learn more at www.PeterAttiaMD.com Connect with Peter on Facebook | Twitter | Instagram.

Perinatal Pharmacokinetics and Serum Methadone/Metabolite Ratios

Addiction Medicine: Beyond the Abstract

Play Episode Listen Later Nov 27, 2018 11:38

In episode nine of Addiction Medicine: Beyond the Abstract, we are joined by Dr. John McCarthy, an associate professor of psychiatry and volunteer clinical faculty at the University of California Davis. In his recent study, Dr. McCarthy and his colleagues examined the significance of serum methadone/metabolite ratios (or MMRs). In particular, they looked at the changes in these ratios for the expecting mother throughout pregnancy and sought to use these ratios to objectively guide methadone dosing. To read the full article, click here.

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Identifying and targeting metabolite detoxifying enzymes to selectively poison cancer cells

Cancer Grand Rounds Lectures from the Norris Cotton Cancer Center Podcasts

Play Episode Listen Later Feb 28, 2018 59:30

Norris Cotton Cancer Center Grand Rounds presented on February 27, 2018 Dohoon Kim, PhD Assistant Professor, University of Massachusetts Medical School

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BacterioFiles 327 - Microbe Metabolite Meliorates Malaise

BacterioFiles

Play Episode Listen Later Feb 5, 2018 9:49

This episode: Gut microbes in mice break down plant foods and produce molecules that stimulate the immune system to resist influenza! Thanks to Drs. Ashley Steed and Thaddeus Stappenbeck for their contributions! Download Episode (9 MB, 9.8 minutes) Show notes: Microbe of the episode: Bougainvillea chlorotic vein banding virus News item Journal Paper: Steed AL, Christophi GP, Kaiko GE, Sun L, Goodwin VM, Jain U, Esaulova E, Artyomov MN, Morales DJ, Holtzman MJ, Boon ACM, Lenschow DJ, Stappenbeck TS. 2017. The microbial metabolite desaminotyrosine protects from influenza through type I interferon. Science 357:498–502. Other interesting stories: Nanobots with propellers modeled after bacterial flagella Embedding useful bacteria into "ink" used to print 3D structures Controlling behavior of individual bacteria using a computer Exercise alone can affect microbiota Microbes in ISS are similar to microbes in Earth homes Email questions or comments to bacteriofiles at gmail dot com. Thanks for listening! Subscribe: iTunes, RSS, Google Play. Support the show at Patreon, or check out the show at Twitter or Facebook

Fighting depression with Ketamine

Research Features Audio

Play Episode Listen Later Oct 25, 2017 7:53

Metabolite created as the body breaks down Ketamine is responsible for its rapid antidepressant action explains Dr Todd Gould from the University of Maryland School of Medicine. To read the full article click here.

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058: Mike Israetel - Q&A - Properly Programming for Metabolite Training