Podcasts about d2r

Creating a world free of poverty on a livable planet has been the vision and mission of the World Bank Group since October 2023. But what does it mean for people and planet? We've just wrapped up our series of podcasts exploring these crucial questions, and in this episode we bring you some of the highlights from the last year. We take you on a journey around the world from Samoa to Nepal and hear from youth activists, refugees, entrepreneurs, development partners and leaders from the World Bank Group. Timestamps[00:00] Welcome and introduction of the topic[02:42] Tackling the climate crisis[05:59] How can we better support refugees?[11:01] What is a green job?[14:34] The journey towards gender equality[17:29] Forests: How can we prosper in harmony with nature?[20:15] Visions of a young World Bank staff memberFeatured VoicesAbdullahi Mire, winner of the 2023 UNHCR Nansen Refugee Award Anna Bjerde, Managing Director of Operations at the World BankAnubha Shukla, Vice President, D2R, Husk Power Cristián Samper, Managing Director and leader of Nature Solutions at Bezos Earth Fund Gita Gopinath, First Deputy Managing Director, International Monetary Fund Inger Anderson, Executive Director, UN Environment Programme Raouf Mazou, Assistant High Commissioner for Operations at UNHCR Manoj Sinha, CEO of Husk Power Maryse Mbonyumutwa, CEO of Pink Mango and Founder of Asantii Nancy Karambo Riungu, a Kenyan entrepreneur and expert tailor working directly with refugees O'Neall Massamba, Transport Unit - Western and Central Africa, World Bank Group Tulshi Suwal, a Nepalese conservationist Valerie Hickey, Global Director of Environment, Natural Resources and Blue Economy, the World Bank Xavier Devictor, Co-Director of the 2023 World Development Report ABOUT THE DEVELOPMENT PODCASTThis international development podcast brings together the data, research—and solutions—that can pave the way to a sustainable future. Through conversations focused on revealing the latest data, the best research, and cutting-edge solutions, let us introduce you to the folks working to make the world a better place. Listen and subscribe for free on your favorite platform. And rate our show! ;) Tell us what you think of our podcast here >>>. We would love to hear from you! ABOUT THE WORLD BANKThe World Bank is one of the world's largest sources of funding and knowledge for low-income countries. Its five institutions share a commitment to reducing poverty, increasing shared prosperity, and promoting sustainable development.

The transition away from fossil fuels could create as many as 30 million ‘green' jobs in clean energy and low-emissions technologies by 2030. So what is a ‘green job'? How can countries and companies support jobs that benefit people and the planet? And how can young people prepare for green jobs of the future? Find out the answers to these questions and more as we speak with Ashish Khanna, Practice Manager, Energy and Extractives, West and Central Africa, World Bank; Manoj Sinha, CEO, Husk Power; Anubha Shukla, Vice President, D2R, Husk Power; and Nidhi Pant, a 2023 winner of the UK's Earthshot Prize and Co-Founder of S4S Technologies as they share their personal stories and examples on why investing in renewable energy is crucial in the fight to create world free of poverty on a livable planet. Tell us what you think of our podcast here >>>. We would love to hear from you! Featured VoicesAshish Khanna, Practice Manager, Energy and Extractives, West and Central Africa, World BankManoj Sinha, CEO, Husk PowerAnubha Shukla, Vice President, D2R, Husk PowerNidhi Pant, a 2023 winner of the UK's Earthshot Prize and Co-Founder of S4S TechnologiesTimestamps[00:00] Welcome and introduction of the topic[02:49] Case study: S4S Technologies, green jobs and value chains[07:27] Case study: Husk Power Systems, gender, renewable power industry, green transition and jobs[11:28] Personal story: Starting a career and a business in the renewable energy sector[15:10] Nigeria and the Distributed Access Through Renewable Energy (DARES) Program[19:11] The link between green jobs and renewable energy[20:03] DARES Nigeria project: Access to electricity and green jobs[22:43] Business models: The role of the local community in terms of innovation[24:41] Overlapping crisis: Green jobs as a part of the recovery to create stability in the world[26:26] ClosureABOUT THE DEVELOPMENT PODCASTThis international development podcast brings together the data, research—and solutions—that can pave the way to a sustainable future. Through conversations focused on revealing the latest data, the best research, and cutting-edge solutions, let us introduce you to the folks working to make the world a better place. Listen and subscribe for free on your favorite platform. And rate our show! ;) Tell us what you think of our podcast here >>>. We would love to hear from you! ABOUT THE WORLD BANKThe World Bank is one of the world's largest sources of funding and knowledge for low-income countries. Its five institutions share a commitment to reducing poverty, increasing shared prosperity, and promoting sustainable development.

Things are getting wet and wild in Yosemite National Park as the agents struggle to keep Mia's unnatural abilities in check. Things get worse when the strangers who are after Tuck's sister come to collect. Can the agents weather this storm? This episode concludes our playthrough of the scenario “Operation: Fulminate, Sentinels of Twilight” by Dennis Detwiller. Congratulations to the winners of our first D2R trivia giveaway! Ren E, NegFour, Darryl T., and Martha M. will all receive Perennial Airlines T-shirts, while Ren E will receive the limited print signed poster! If you missed out, stay tuned for ep 26, when we will hold another trivia contest. TRIGGER AND CONTENT WARNINGS: Violence, blood, and gore, mutilation, death, body horror, mental illness, Dissociative Identity Disorder, PTSD, manipulation, gaslighting, religious trauma, infighting, the topic of Covid-19 including conspiracy theorists, drug and alcohol use, gun and knife violence, sexual situations specifically under the influence, surgery discussions, legal proceedings, mentions of racially insensitive folk tales, threats against the life of a child, medical procedures, parental disagreements. Published by arrangement with the Delta Green Partnership. The intellectual property known as Delta Green is a trademark and copyright owned by the Delta Green Partnership, who has licensed its use here. The contents of this podcast are © Mayday Roleplay, excepting those elements that are components of the Delta Green intellectual property. CAST OF CHARACTERS • Lev (they/them) - Haley Moore, Agent Tuck (she/they) • Amanda (she/her) - Maxine Parker, Agent Boomer (she/her) • Caleb (he/him) - Orson Bradham, Agent Merit (he/him) • Eli (any/all) - Kona Morales, Agent Hyde (she/her) • Zakiya (she/they) - Gaze, Agent Warp (she/they) • Sergio (he/him) - The Handler MUSIC & SOUND EFFECTS • Post Sound Supervision: Sergio Crego, Eli Hauschel • Mixed: Eli Hauschel • Original Music: Aaron A. Pabst • Soundstripe (soundstripe.com) • Epidemic Sound (epidemicsound.com/) • Soundly (getsoundly.com/) DELTA GREEN LINKS • Delta Green (http://deltagreen.com/) • Operation FULMINATE: Sentinels of Twilight (https://drivethrurpg.com/en/product/456340/) MAYDAY ROLEPLAY LINKS • Patreon (https://www.patreon.com/maydayrp) • Ko-Fi (https://ko-fi.com/maydayrp) • Mayday website (https://www.maydayroleplay.com/) • Youtube (https://www.youtube.com/@Maydayrp)

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2023.07.24.550350v1?rss=1 Authors: Tosta, A. S., Fonseca, A. S., Messeder, D., Siqueira, P., Ferreira, S. T., Lourenco, M. V., Pandolfo, P. Abstract: Attention deficit hyperactivity disorder (ADHD) is characterized by inattention, hyperactivity and impulsivity, and develops most frequently during childhood and adolescence. Spontaneously hypertensive rats (SHR) are the most used experimental model for the study of ADHD. SHR exhibit behavioral impairments that recapitulate phenotypes observed in individuals with ADHD. SHR further develop dopaminergic hypofunction in frontostriatal circuits and an imbalance in dopamine and norepinephrine systems. Maternal physical exercise (e.g., swimming) during pregnancy has been shown to promote angiogenesis, neurogenesis, learning, and memory in the offspring of control rats. We investigated the impact of gestational swimming on behavioral and dopaminergic parameters in childhood (1-2 weeks of age) and adolescent (4-5 weeks of age) SHR and Wistar Kyoto rats (WKY), used as a control. Maternal gestational swimming resulted in a reversal of neurodevelopmental impairments in behavior, assessed by the righting reflex and olfactory recognition tests, in the offspring. Furthermore, during adolescence, SHRs from exercised dams exhibited reduced novelty seeking, an important behavioral trait in this developmental period. Finally, SHRs exhibited increased expression of dopamine transporter (DAT) and D2 receptors (D2R) in the frontal cortex. D2R expression was normalized in the frontal cortex of adolescent SHRs whose mothers were exercised. Results suggest that physical exercise during pregnancy could be an effective preventative strategy against ADHD-associated behavioral and neurochemical phenotypes in the offspring. Copy rights belong to original authors. Visit the link for more info Podcast created by Paper Player, LLC

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2023.06.21.545877v1?rss=1 Authors: Lukkarinen, L., Tuisku, J., Sun, L., Helin, S., Karlsson, H., Venetjoki, N., Salomaa, M., Rautio, P., Hirvonen, J., Lauerma, H., Tiihonen, J., Nummenmaa, L. Abstract: Background: Psychopathy is characterized by antisocial behavior, poor behavioral control and lacking empathy, and structural alterations in the corresponding neural circuits. Molecular brain basis of psychopathy remains poorly characterized. Methods: Here we studied type 2 dopamine receptor (D2R) and mu-opioid receptor (MOR) availability in convicted violent offenders with high psychopathic traits (n=11) and healthy matched controls (n=19) using positron emission tomography (PET). D2R were measured with radioligand [11C]raclopride and MORs with radioligand [11C]carfentanil. Results: Psychopathic subjects had lowered D2R availability in caudate and putamen, and D2R striatal availability was also associated with degree of psychopathic traits in this prisoner sample. No group differences were found in MOR availability, although in the prisoner sample, psychopathic traits were negatively correlated with MOR availability amygdala and nucleus accumbens. Conclusions: We conclude that D2R signaling could be the putative neuromolecular pathway for psychopathy, whereas evidence for the aberrant MOR system is more limited. Copy rights belong to original authors. Visit the link for more info Podcast created by Paper Player, LLC

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2023.05.04.539488v1?rss=1 Authors: Gayden, J., Puig, S., Srinivasan, C., Buck, S. A., Gamble, M. C., Glausier, J. R., Tejeda, H. A., Dong, Y., Pfenning, A. R., Logan, R., Freyberg, Z. Abstract: Striatal dopamine (DA) neurotransmission is critical for an array of reward-related behaviors and goal-directed motor control. In rodents, 95% of striatal neurons are GABAergic medium spiny neurons (MSNs) that have been traditionally segregated into two subpopulations based on the expression of stimulatory DA D1-like receptors versus inhibitory D2-like receptors. However, emerging evidence suggests that striatal cell composition is anatomically and functionally more heterogenous than previously appreciated. The presence of MSNs that co-express multiple DA receptors offers a means to more accurately understand this heterogeneity. To dissect the precise nature of MSN heterogeneity, here we used multiplex RNAscope to identify expression of three predominantly expressed DA receptors in the striatum: DA D1 (D1R), D2 (D2R), and D3 (D3R) receptors. We report heterogenous subpopulations of MSNs that are distinctly distributed across the dorsal-ventral and rostral-caudal axes of the adult mouse striatum. These subpopulations include MSNs that co-express D1R and D2R (D1/2R), D1R and D3R (D1/3R), and D2R and D3R (D2/3R). Overall, our characterization of distinct MSN subpopulations informs our understanding of region-specific striatal cell heterogeneity. Copy rights belong to original authors. Visit the link for more info Podcast created by Paper Player, LLC

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2023.05.05.539468v1?rss=1 Authors: Gulfo, M. C., Lebowitz, J. J., Ramos, C., Hwang, D.-W., Nasrallah, K., Castillo, P. E. Abstract: Hilar mossy cells (MCs) are principal excitatory neurons of the dentate gyrus (DG) that play critical roles in hippocampal function and have been implicated in brain disorders such as anxiety and epilepsy. However, the mechanisms by which MCs contribute to DG function and disease are poorly understood. Expression from the dopamine D2 receptor (D2R) gene (Drd2) promoter is a defining feature of MCs, and previous work indicates a key role for dopaminergic signaling in the DG. Additionally, the involvement of D2R signaling in cognition and neuropsychiatric conditions is well-known. Surprisingly, though, the function of MC D2Rs remain largely unexplored. In this study, we show that selective and conditional removal of Drd2 from MCs of adult mice impaired spatial memory, promoted anxiety-like behavior and was proconvulsant. To determine the subcellular expression of D2Rs in MCs, we used a D2R knockin mouse which revealed that D2Rs are enriched in the inner molecular layer of the DG, where MCs establish synaptic contacts with granule cells. D2R activation by exogenous and endogenous dopamine reduced MC to dentate granule cells (GC) synaptic transmission, most likely by a presynaptic mechanism. In contrast, removing Drd2 from MCs had no significant impact on MC excitatory inputs and passive and active properties. Our findings support that MC D2Rs are essential for proper DG function by reducing MC excitatory drive onto GCs. Lastly, impairment of MC D2R signaling could promote anxiety and epilepsy, therefore highlighting a potential therapeutic target. Copy rights belong to original authors. Visit the link for more info Podcast created by Paper Player, LLC

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2023.04.02.535293v1?rss=1 Authors: Zimbelman, A. R., Wong, B., Murray, C. H., Wolf, M. E., Stefanik, M. T. Abstract: Protein translation is essential for some forms of synaptic plasticity. We used nucleus accumbens (NAc) medium spiny neurons (MSN), co-cultured with cortical neurons to restore excitatory synapses, to examine whether dopamine modulates protein translation in NAc MSN. FUNCAT was used to measure translation in MSNs under basal conditions and after disinhibiting excitatory transmission using the GABAA receptor antagonist bicuculline (2 hr). Under basal conditions, translation was not altered by the D1-class receptor (D1R) agonist SKF81297 or the D2-class receptor (D2R) agonist quinpirole. Bicuculline alone robustly increased translation. This was reversed by quinpirole but not SKF81297. It was also reversed by co-incubation with the D1R antagonist SCH23390, but not the D2R antagonist eticlopride, suggesting dopaminergic tone at D1Rs. This was surprising because no dopamine neurons are present. An alternative explanation is that bicuculline activates translation by increasing glutamate tone at NMDA receptors (NMDAR) within D1R/NMDAR heteromers, which have been described in other cell types. Supporting this, immunocytochemistry and proximity ligation assays revealed D1/NMDAR heteromers on NAc cells both in vitro and in vivo. Further, bicuculline's effect was reversed to the same extent by SCH23390 alone, the NMDAR antagonist APV alone, or SCH23390+APV. These results suggest that: 1) excitatory synaptic transmission stimulates translation in NAc MSNs, 2) this is opposed when glutamate activates D1R/NMDAR heteromers, even in the absence of dopamine, and 3) antagonist occupation of D1Rs within the heteromers prevents their activation. Our study is the first to suggest a role for D2 receptors and D1R/NMDAR heteromers in regulating protein translation. Copy rights belong to original authors. Visit the link for more info Podcast created by Paper Player, LLC

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2023.01.12.523788v1?rss=1 Authors: Rocchetti, J., Fasano, C., Dal-Bo, G., Guma, E., El Mestikawy, S., Wong, T. P., Fakhfouri, G., GIROS, B. Abstract: Evidence suggests that subcortical hyperdopaminergia alters cognitive function in schizophrenia and antipsychotic drugs (APD) fail at rescuing cognitive deficits in patients. In a previous study, we showed that blocking D2 dopamine receptors (D2R), a core action of APD, led to profound reshaping of mesohippocampal fibers, deficits in synaptic transmission and impairments in learning and memory in the mouse hippocampus (HP). However, it is currently unknown how excessive dopamine affects HP-related cognitive functions, and how APD would impact HP functions in such a state. After verifying the presence of DAT-positive neuronal projections in the ventral (temporal), but not in the dorsal (septal), part of the HP, GBR12935, a blocker of dopamine transporter (DAT), was infused in the CA1 of adult C57Bl/6 mice to produce local hyperdopaminergia. Chronic GBR12935 infusion in temporal CA1 induced a mild learning impairment in the Morris Water Maze and abolished long-term recognition memory in novel-object (NORT) and object-place recognition tasks (OPRT). Deficits were accompanied by a significant decrease in DAT+ mesohippocampal fibers. Intrahippocampal or systemic treatment with sulpiride during GBR infusions improved the NORT deficit but not that of OPRT. In vitro application of GBR on hippocampal slices abolished long-term depression (LTD) of fEPSP in temporal CA1. LTD was rescued by co-application with sulpiride. In conclusion, chronic DAT blockade in temporal CA1 profoundly altered mesohippocampal modulation of hippocampal functions. Contrary to previous observations in normodopaminergic mice, antagonising D2Rs was beneficial for cognitive functions in the context of hippocampal hyperdopaminergia. Copy rights belong to original authors. Visit the link for more info Podcast created by Paper Player, LLC

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2022.10.12.511974v1?rss=1 Authors: Boxer, E., Kim, J., Dunn, B., Aoto, J. Abstract: Ventral subiculum (vSUB) is the major output region of ventral hippocampus (vHIPP) and sends major projections to nucleus accumbens medial shell (NAcMS). Hyperactivity of the vSUB-NAcMS circuit is associated with substance use disorders (SUDs) and the modulation of vSUB activity alters drug seeking and drug reinstatement behavior in rodents. However, to the best of our knowledge, the cell-type specific connectivity and synaptic transmission properties of the vSUB-NAcMS circuit have never been directly examined. Instead, previous functional studies have focused on total ventral hippocampal (vHIPP) output to NAcMS without distinguishing vSUB from other subregions of vHIPP, including ventral CA1 (vCA1). Using ex vivo electrophysiology, we systematically characterized the vSUB-NAcMS circuit with cell-type and synapse specific resolution in male and female mice and found that vSUB output to dopamine receptor type-1 (D1R) and type-2 (D2R) expressing medium spiny neurons (MSNs) displays a functional connectivity bias for D2R MSNs. Furthermore, we found that vSUB-D1R and -D2R MSN synapses contain calcium-permeable AMPA receptors in drug-naive mice. Finally, we find that, distinct from other glutamatergic inputs, cocaine exposure selectively induces plasticity at vSUB-D2R synapses. Importantly, we directly compared vSUB and vCA1 output to NAcMS and found that vSUB synapses are functionally distinct and that vCA1 output recapitulated the synaptic properties previously ascribed to vHIPP. Our work highlights the need to consider the contributions of individual subregions of vHIPP to SUDs and represents an important first step toward understanding how the vSUB-NAcMS circuit contributes to the etiologies that underlie SUDs. Copy rights belong to original authors. Visit the link for more info Podcast created by PaperPlayer

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2022.09.10.507436v1?rss=1 Authors: Onimus, O., Valjent, E., Fisone, G., Gangarossa, G. Abstract: Antipsychotics share the common pharmacological feature of antagonizing the dopamine 2 receptor (D2R) which is abundant in the striatum and involved in both the therapeutic and side effects of this drugs class. Pharmacological blockade of striatal D2R, by disinhibiting the D2R-containing medium-size spiny neurons (MSNs), leads to a plethora of molecular, cellular and behavioral adaptations which are central in the action of antipsychotics. Here, we focused on the cell type-specific (D2R-MSNs) regulation of some striatal immediate early genes (IEGs), such as cFos, Arc and Zif268. Taking advantage of transgenic mouse models, pharmacological approaches and immunofluorescence analyses, we found that haloperidol-induced IEGs in the striatum required the synergistic activation of A2a (adenosine) and NMDA (glutamate) receptors. At the intracellular signaling level, we found that the PKA/DARPP-32 and mTOR pathways synergistically cooperate to control the induction of IEGs by haloperidol. By confirming and further expanding previous observations, our results provide novel insights into the regulatory mechanisms underlying the molecular/cellular action of antipsychotics in the striatum. Copy rights belong to original authors. Visit the link for more info Podcast created by PaperPlayer

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2022.09.09.507130v1?rss=1 Authors: Chantranupong, L., Beron, C. C., Zimmer, J. A., Wen, M. J., Wang, W., Sabatini, B. L. Abstract: Within the basal ganglia, striatal dopamine (DA) and acetylcholine (Ach) are essential for the selection and reinforcement of motor actions and decision making. In vitro studies have revealed a circuit local to the striatum by which each of these two neurotransmitters directly regulates release of the other. Ach, released by a unique population of cholinergic interneurons (CINs), drives DA release via direct axonal depolarization. In turn, DA inhibits CIN activity via dopamine D2 receptors (D2R). Whether and how this circuit contributes to striatal function in vivo remains unknown. To define the in vivo role of this circuit, we monitored Ach and DA signals in the ventrolateral striatum of mice performing a reward-based decision-making task. We establish that DA and Ach exhibit multiphasic and anticorrelated transients that are modulated by decision history and reward outcome. However, CIN perturbations reveal that DA dynamics and reward-prediction error encoding do not require Ach release by CINs. On the other hand, CIN-specific deletion of D2Rs shows that DA inhibits Ach levels in a D2R-dependent manner, and loss of this regulation impairs decision-making. To determine how other inputs to striatum shape Ach signals, we assessed the contribution of projections from cortex and thalamus and found that glutamate release from both sources is required for Ach release. Altogether, we uncover a dynamic relationship between DA and Ach during decision making and reveal modes of CIN regulation by local DA signals and long-range cortical and thalamic inputs. These findings deepen our understanding of the neurochemical basis of decision making and behavior. Copy rights belong to original authors. Visit the link for more info Podcast created by PaperPlayer

Amazon and their game giveaways for Prime Day. New data on how gamers feel about certain brands. Epic gives us a tool to play nice with Steam players. Insults online put you in jail in Japan. Working in VR sucks. You can finally get a 3080 at a decent price. Scott played Grim Dawn, D2R, D3, Fall Guys, Raft, and V Rising. Jon played Shredders Revenge, FFVII Remake, and Vampire Survivors. Beau played FFVII Remake, D3 Necro, and is learning C# for fun! Plus your emails and a great mashup!

Amazon and their game giveaways for Prime Day. New data on how gamers feel about certain brands. Epic gives us a tool to play nice with Steam players. Insults online put you in jail in Japan. Working in VR sucks. You can finally get a 3080 at a decent price. Scott played Grim Dawn, D2R, D3, Fall Guys, Raft, and V Rising. Jon played Shredders Revenge, FFVII Remake, and Vampire Survivors. Beau played FFVII Remake, D3 Necro, and is learning C# for fun! Plus your emails and a great mashup!

For more details, visit the #DrGPCR Podcast Episode #71 page https://www.drgpcr.com/episode-71-with-dr-jean-martin-beaulieu ------------------------------------------- About Dr. Jean Martin Beaulieu Dr. Beaulieu received a Ph.D. in Neurological Sciences from McGill University and completed his post-doctoral training at Duke University. Prior to his recruitment Dr. Beaulieu was an associate professor and Canada Research Chair (Tier2) in the Department of Psychiatry and Neuroscience at Laval University. Dr. Beaulieu's research is aimed at understanding how cellular and molecular mechanisms regulated by psychoactive drugs intersect with genetic risk factors for mental illnesses such as schizophrenia, depression, and bipolar disorder. Dr. Beaulieu has pioneered work establishing a role for Beta-arrestin signaling in the brain in vivo and has established its importance in D2 dopamine receptors (D2R) functions. These receptors belong to the super-family of G-protein coupled receptors (GPCR), the major molecular target for drug development. In particular, D2R is the main pharmacological target of antipsychotic drugs prescribed for schizophrenia and bipolar disorders. Work by the Beaulieu Lab has demonstrated that mood stabilizer drugs (e.g. lithium) used for bipolar disorder therapy target signaling mechanisms regulated by dopamine receptors, thus providing a framework to understand how different drug classes can engage overlapping cellular mechanisms to exert their action. The Beaulieu group is presently investigating how cell surface express proteins can act as allosteric modulators of D2R signaling and explores the potential usefulness of beta-arrestins for the development of new pharmaceutical agents. Translational validation is important to validate findings obtained from experimental models research and bridge the gap between bench and bedside. Working in collaboration with geneticists, the Beaulieu-Lab has identified interactions between cellular mechanisms engaged by D2R and psychiatric drugs with genetic risk factors implicated in schizophrenia by large whole-genome association studies (GWAS) in humans. These investigations have led to the identification of an RNA binding protein (FXR1P) involved in the regulation of protein synthesis as a potential downstream effector of the action of mood stabilizers and other psychoactive drugs. In addition to basic research, the Beaulieu group is also actively implicated in translational research and industry collaboration to develop new drugs and drug development technology. Dr. Jean Martin Beaulieu on the web University of Toronto Google Scholar LinkedIn ResearchGate ------------------------------------------- We aspire to provide opportunities to connect, share, form trusting partnerships, grow, and thrive together. Fill out the Ecosystem waitlist form today to be the first to explore our brand new and improved space! For more details, visit our website http://www.DrGPCR.com/Ecosystem/.

Knocking the rust off and discussing D2R, D3, D4, and immortal.

D2R: Alpha Test Beans [Link] Blizzard has been listening to player feedback to preserve the classic nature and spirit of the game. “There are so many unique quirks and systems in this game that, if modernized, would ultimately sabotage the original experience we adore. So, we've exercised restraint to protect the authentic experience” VFX, QoL, Accessibility, Interface (like auto gold pickup), shared stash, ‘compare' tooltips, automap visualization enhancements, a clock (LOL), Loading Screen: Character Vulnerability & Time (no more getting 1 shot before you even load) For a limited time in August, players who pre-ordered D2R will be able to step into Sanctuary in an early access beta. “Soon after,” all players (on PC, Xbox, and PlayStation) will have the chance to play in the open beta. We'll be able to play the Amazon, Barbarian, Paladin, Sorceress, and Druid. No assassin or necro mentions yet! Party with up to 8 players. Discord Goes Artificial [Link] Discord has purchased AI ant- harassment company Sentropy. Sentropy's product-in-mention, Sentropy Protect, monitors networks for abuse/harassment, then offers users a way to block problematic people and filter out messages they don't want to see. The product was initially focused on helping people clean up their Twitter feeds and to create tools for companies to support moderation. As of now, Discord has a huge network of more than 150 million monthly users! The userbase is currently made up of more than 19 million communities and requires the work of both volunteers and teams within Discord to properly moderate. I'm sure a LOT of testing and “retrofitting” of Sentropy's algorithms will have to take place before we see it in action. Mega Sega-suit [Link] Sega is catching some heat right now under a lawsuit claiming that one of its arcade games, Key Master, is actually rigged! ..and this isn't the first time the game has come under fire.. The plaintiff, Marcelo Muto, is suing the company for $5 million Key Master, which is found in arcades and malls across the US, has players use a joystick and button to maneuver a key into a specific hole where winners are awarded with prizes: such as earbuds, video games and even GoPros. If a player gets the key into the correct position to win, but not within the predetermined time, the machine will overshoot, causing a loss. Pretty simple, right? Each machine, the lawsuit says, is programmed to only allow a win after a certain number of losses, 700 by default. This denies players from winning with actual skill. That's a lot of Pipe [Link] An anonymous bidder purchased a copy of Mario 64 for 1.56 million (Valarie McLeckie told NPR) This was a high value sale due to nostalgia of Nintendo's iconic mascot. Also, unexpectedly raising speculation and prices during the pandemic Broke a previous record two days prior held by 1987 Legend of Zelda that sold for $870,000 --- This episode is sponsored by · Anchor: The easiest way to make a podcast. https://anchor.fm/app Support this podcast: https://anchor.fm/saved-state-heros/support

Shazam talks about potential upcoming D2R beta and his impressions of the Diablo Immortal Alpha sofar.

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2020.10.21.348995v1?rss=1 Authors: Liput, D. J., Puhl, H. L., Dong, A., He, K., Li, Y., Lovinger, D. M. Abstract: Several forms of endocannabinoid (eCB) signaling have been described in the dorsal lateral striatum (DLS), however most experimental protocols used to induce plasticity do not recapitulate the firing patterns of striatal-projecting pyramidal neurons in the cortex or firing patterns of striatal medium spiny neurons. Therefore, it is unclear if current models of eCB signaling in the DLS provide a reliable description of mechanisms engaged under physiological conditions. To address this uncertainty, we investigated mechanisms of eCB mobilization following brief synaptic stimulation that mimics in vivo patterns of neural activity in the DLS. To monitor eCB mobilization, the novel genetically encoded fluorescent eCB biosensor, GRABeCB2.0, was expressed in corticostriatal afferents of C57BL6J mice and evoked eCB transients were measured in the DLS using a brain slice photometry technique. We found that brief bouts of synaptic stimulation induce long lasting eCB transients. Inhibition of monoacylglycerol lipase, prolonged the duration of the eCB transient, while inhibition of diacylglycerol lipase inhibited the peak amplitude, suggesting that 2-AG is the predominate eCB generated following brief synaptic stimulation. 2-AG transients were robustly inhibited by AMPA and NMDA receptor antagonists, DNQX and DL-AP5 respectively. Additionally, the 2-AG transient was inhibited by the muscarinic M1 receptor (M1R) antagonist, VU 0255035, and augmented by the M1R positive allosteric modulator, VU 0486846, indicating that acetylcholine (ACh) release is required for efficient 2-AG production. The dopamine D2 receptor (D2R) agonist, quinpirole, inhibited the 2-AG transient. However, in slices from mice lacking D2Rs on cholinergic interneurons (CINs), quinpirole did not inhibit the 2-AG transient, demonstrating that D2Rs on CINs can modulate 2-AG production. The AMPA receptor or NMDA receptor antagonists, DNQX or DL-AP5 respectively, occluded 2-AG augmentation by VU 0486846 suggesting that converging glutamatergic and cholinergic signals are required for efficient 2-AG production following brief synaptic stimulation. Collectively, these data uncover unrecognized mechanisms underlying 2-AG mobilization in the DLS. Copy rights belong to original authors. Visit the link for more info

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2020.09.25.312967v1?rss=1 Authors: Braco, J. T., Saunders, C. J., Nelson, J. M., Johnson, E. C. Abstract: In insects, Adipokinetic hormone is the primary hormone responsible for the mobilization of stored energy. While a growing body of evidence has solidified AKHs role in modulating the physiological and behavioral responses to metabolic stress, little is known about the upstream endocrine circuit that directly regulates AKH release. We evaluated the AKH-expressing cell transcriptome to identify potential regulatory elements controlling AKH cell activity, and found that a number of receptors show consistent expression levels, including all known dopamine receptors, dopamine ecdysone receptor (DopEcR), Dopamine 2-like receptor (D2R), Dopamine 1-like receptor 2 (DopR2), DopR, and the Pigment Dispersing Factor (PDFR). We tested the consequences of targeted genetic knockdown and found that RNAi elements targeting each dopamine receptor caused a significant reduction in survival under starvation. In contrast, PDFR knockdown significantly extended lifespan under starvation whereas expression of a tethered PDF in AKH cells resulted in a significantly shorter lifespan during starvation. These manipulations also caused various changes in locomotor activity under starvation. Specifically, there were higher amounts of locomotor activity in dopamine receptor knockdowns, in both replete and starved states. PDFR knockdown resulted in increased locomotion during replete conditions and locomotion levels that were comparable to wild-type during starvation. Expression of a membrane-tethered PDF led to decreased locomotion under baseline and starvation. Next, we used live-cell imaging to evaluate the acute effects of the ligands for these receptors (dopamine, ecdysone, and Pigment Dispersing Factor) on AKH cell activation. Dopamine application led to a transient increase in intracellular calcium in a sugar-dependent manner. Furthermore, we found that co-application of dopamine and ecdysone led to a complete loss of this response, suggesting that these two hormones are acting antagonistically. We also found that PDF application directly led to an increase in cAMP in AKH cells, and that this response was dependent on expression of the PDFR in AKH cells. Together these results suggest a complex circuit in which multiple hormones act on AKH cells to modulate metabolic state. Copy rights belong to original authors. Visit the link for more info

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2020.09.07.284612v1?rss=1 Authors: Gallo, E. F., Greenwald, J., Teboul, E., Martyniuk, K. M., Li, Y., Javitch, J. A., Balsam, P. D., Kellendonk, C. Abstract: Cholinergic interneurons (CINs) in the striatum respond to salient stimuli with a multiphasic response, including a pause, in neuronal activity. Slice physiology experiments have shown the importance of dopamine D2 receptors (D2Rs) in regulating CIN pausing yet the behavioral significance of the CIN pause and its regulation by dopamine in vivo is still unclear. Here, we show that D2R upregulation in CINs of the nucleus accumbens (NAc) lengthens the pause in CIN activity ex vivo and enlarges a stimulus-evoked decrease in acetylcholine (ACh) levels during behavior. This enhanced dip in ACh levels is associated with a selective deficit in the learning to inhibit responding in a Go/No-Go task. Our data demonstrate, therefore, the importance of CIN D2Rs in modulating the CIN response induced by salient stimuli and points to a role of the pause in inhibitory learning. This work has important implications for brain disorders with altered striatal dopamine and ACh function, including schizophrenia and attention-deficit hyperactivity disorder (ADHD). Copy rights belong to original authors. Visit the link for more info

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2020.06.18.160416v1?rss=1 Authors: Ramirez-Jarquin, U. N., Shahani, N., Pryor, W., Usiello, A., Subramaniam, S. Abstract: The mammalian target of rapamycin (mTOR) is a ubiquitously expressed serine/threonine kinase protein complex (mTORC1 or mTORC2) that orchestrates diverse functions ranging from embryonic development to aging. However, its brain tissue-specific roles remain less explored. Here, we have identified that the depletion of the mTOR gene in the mice striatum completely prevented the extrapyramidal motor side-effects (catalepsy) induced by the dopamine 2 receptor (D2R) antagonist haloperidol, which is the most widely used typical antipsychotic drug. Conversely, a lack of striatal mTOR in mice did not affect catalepsy triggered by the dopamine 1 receptor (D1R) antagonist SCH23390. Along with the lack of cataleptic effects, the administration of haloperidol in mTOR mutants failed to increase striatal phosphorylation levels of ribosomal protein pS6 (S235/236) as seen in control animals. To confirm the observations of the genetic approach, we used a pharmacological method and determined that the mTORC1 inhibitor rapamycin has a profound influence upon post-synaptic D2R-dependent functions. We consistently found that pretreatment with rapamycin entirely prevented (in a time-dependent manner) the haloperidol-induced catalepsy in wild-type mice. Collectively, our data indicate that striatal mTORC1 blockade may offer therapeutic benefits with regard to the prevention of D2R-dependent extrapyramidal motor side-effects of haloperidol in psychiatric illness. Copy rights belong to original authors. Visit the link for more info

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2020.05.04.077792v1?rss=1 Authors: Green, S., Nathani, S., ZImmerman, J., Fireman, D., Urs, N. M. Abstract: The cortex plays an important role in regulating motivation and cognition, and does so by regulating multiple subcortical brain circuits. Glutamatergic pyramidal neurons in the prefrontal cortex (PFC) are topographically organized in different subregions such as the prelimbic, infralimbic and orbitofrontal, and project to topographically-organized subcortical target regions. Dopamine D1 and D2 receptors are expressed on glutamatergic pyramidal neurons in the PFC. However, it is unclear whether D1 and D2 receptor-expressing pyramidal neurons in the PFC are also topographically organized. We used a retrograde adeno-associated virus (AAVRG)-based approach to illuminate the topographical organization of D1 and D2 receptor-expressing neurons, projecting to distinct striatal and midbrain subregions. Our experiments reveal that AAVRG injection in the nucleus accumbens (NAcc) or dorsal striatum (dSTR) of D1Cre mice labeled distinct neuronal subpopulations in medial orbitofrontal or prelimbic PFC, respectively. However, AAVRG injection in NAcc or dSTR of D2Cre mice labeled medial orbitofrontal, but not medial prelimbic PFC, respectively. Additionally, D2R+ but not D1R+ PFC neurons were labeled upon injection of AAVRG in substantia nigra pars compacta (SNpc). Thus, our data are the first to highlight a unique dopamine receptor-specific topographical pattern in the PFC, which could have profound implications for cortico-striatal signaling in the basal ganglia. Copy rights belong to original authors. Visit the link for more info

While 'The Thunda' is getting a much needed break with his family, Tommy from The Golden Dice Podcast (and future father of two) joins Mr. Chip and Josh aka Bananacrapshoot on this special post-GenCon episode. An unexpected visitor - Palpoo AKA Mike Gemme AKA Bobby Sapphire joins D2R to talk about his recent victory at the 2019 North American Championship, huge wooden dice, decklist photography, and why diceless decks make for winning decks. Thanks to all of our Reroller Patrons for making this show possible - patreon.discardtoreroll.com. All-new D2R gear is now available at shop.discardtoreroll.com = and join our 100% free discord at discord.discardtoreroll.com.

Al Lopez is a fourth-generation coffee farmer, so it’s fitting that today’s episode is recorded in a coffee shop. Al has one of the most amazing stories you’ve never heard, and as he and Kevin share a few cups of Alma Coffee, you’ll discover the heart-filled lengths Al went to as he gave back to his community in Honduras. Journey to the United States Al grew up with coffee trees in his backyard, and it was his grandfather who instilled a love for them. He shares the story of how his grandfather encouraged him to pick and process the beans by hand, then took it a step further by allowing Al to sell the coffee and keep the profits. His father moved to the United States and Al joined him there on the promise of his own bicycle, and he shares the story of what it was like coming to a place with airplanes, electricity, indoor plumbing, and Coca-Cola in a can. He also shares the troubles he faced being ‘different’ in public schools, how he ended up in the military, and the injury that landed him in a factory. Inspiration for the Future While working in the factory, Al’s foreman kept telling him, “You have a head on your shoulders. Go to school.” So he did. From junior college to university, Al graduated with an accounting degree. As he waited for his first job interview, he had the crushing feeling that he wasn’t good enough to clean the bathrooms. Despite that, he had a phenomenal career there and moved to Sara Lee, where he really took off. He credits part of his success to growing up ‘on the streets.’ The Second Mountain Al retired from his CEO position at age 42, having achieved his biggest goal. But he wasn’t finished yet; Al had a new purpose and it had nothing to do with his ego. He found himself being drawn back to his home in Honduras. For 800 years, farmers sold coffee and starved to death; they weren’t the ones making the money. Al decided to change that, and he shares the story of how he created Alma Coffee based on the idea of D2R, or ‘direct to roaster.’ But his holistic approach to helping the farmers goes much deeper. The Real Deal Al’s company was built, and he needed to find a roaster. He partnered with PJ’s Coffee in New Orleans after taking them to his farm in Honduras. He showed them that the story of his company, how he treats employees, and the difference it makes in the community was much more than a marketing ploy. It was the real deal because Al puts people first. He shares the three foundational pillars: improving lives, sustainable farming, and exceptional coffee. Al also shares his vision for the future; he wants the farming operation to continue 300 years after he’s gone. The Definition of Success Before Alma Coffee, the definition of success was the size of the paycheck. The golden parachute. Flying in private jets. But today, Al measures his success very differently: his farm director being able to buy his own home; his employees always having food, shelter, and community; lifting up the small town where he came from by improving the quality of life; his children wanting to continue the legacy. These things are the new definition of success. Resources Alma Coffee | Alma Coffee on Facebook | PJ’s Coffee | “The Second Mountain” by David Brooks

Dreem2Reality Entertainment presents The Rock Vegas Podcast. On today's show: Ryan and Dave host today's one hundred twenty ninth episode! The guys start the show by discussing the Singing Trump on America's Got Talent. Then, the guys marvel over the impressions of Jonathan Kite and Ryan Goldsher before Ryan presents a perplexing situation he's come across. Ryan has discovered that an acquaintance has allegedly stolen the intellectual concept of D2R and Dreem2Reality that Ryan created several years ago, and thsi acquaintance has gone ahead and used it to title a rap album and gone so far to title a track on the album D2R. Ryan tells the whole story and debates on what he should do about this perplexing situation. Enjoy the eargasms! Please subscribe to the D2R Podcast Network on iTunes and don't forget to rate and review while you're there. You can also download the Stitcher and Podbean app to your device for free and search: D2R PODCAST NETWORK and subscribe. If you enjoy listening to The Rock Vegas Podcast, then spread the word to everyone you know. Your word of mouth is our best advertising method and we appreciate your support. Thanks for listening and share!

On today's show: Ryan hosts an all new episode from the Dreem2Reality Entertainment Studio with his co-hosts Dani, Jon, Dave, Ray, and Jamie.The show starts with Ryan introducing what he considers to be his co-host heavy hitters for the very special 100th episode! Ryan puts Jamie on the spot to discuss his experiences with the D2R Podcast from the very beginning right up to today. Then, Jon talks about being nervous to getting drunk and then really lost in his experiences on the podcast. And after that, Dani gets her turn to discuss her thoughts on the D2R crew and everything she's learned from them. Then, Dave speaks his mind on what it was like going from a guest to a fill-in co-host to getting his own podcast on the D2R Network. And then, last but not least, Ray discusses going from the unpaid camera operator to an unpaid on-air talent. After a ton a fun and hilarious stories by everyone, Ryan leads the crew into a "Would You Rather" discussion and it's by far, the most revealing episode for every D2R crew member to date! And then, to round out the show, one member of the D2R crew bids farewell to the D2R Podcast crew and listeners. Enjoy!Please SUBSCRIBE to the D2R Podcast on iTunes and don't forget to RATE and REVIEW while you're there.Be a hero - share the D2R Podcast with everyone you know! Your word of mouth is our only advertising and we truly appreciate your support! Thanks for listening and enjoy!!

On today's show: Ryan hosts an all new episode from the Dreem2Reality Entertainment Studio with his co-hosts Dani, Jon, Dave, Ray, and Jamie.The 2nd Annual D2Ry Awards ceremony features 62 categories with winners voted on by you, the listener, as well as the D2R crew and guest alumni. It's a long one, so settle in, grab some popcorn and enjoy!Coming up next week: the D2R Podcast celebrates its 100th Episode!!!Please SUBSCRIBE to the D2R Podcast on iTunes and don't forget to RATE and REVIEW while you're there.Be a hero - share the D2R Podcast with everyone you know! Your word of mouth is our only advertising and we truly appreciate your support! Thanks for listening and enjoy!!

On today's show: Ryan hosts an all new episode from the Dreem2Reality Entertainment Studio with his co-hosts Dani, Jon, and Ray.The show starts with a "How Oreo's Are Made" segment, then some fun Oreo facts which gets interrupted by Dani having an accident involving tequila. After that catastrophe, more fun Oreo facts and every Oreo flavor that's been created-to-date are discussed. A little bit of Oreo history is dropped for the listener and then the moment you've all been waiting for...the Oreo taste testing!! After the taste test, the crew ranks the Oreo's. All of this is set to multiple Oreo-related tunes and just in time for National Oreo Day which is March 6. Enjoy!On a side-note, at least 2 members of the D2R crew were injured during the making of this episode, however, everybody is doing fine as of this episode's release!Please SUBSCRIBE to the D2R Podcast on iTunes and don't forget to RATE and REVIEW while you're there.Be a hero - share the D2R Podcast with everyone you know! Your word of mouth is our only advertising and we truly appreciate your support! Thanks for listening and enjoy!!

On today's show: Ryan hosts an all new episode from the Dreem2Reality Entertainment Studio with his co-hosts Dani, Jon, and Ray.Ryan starts the show by wishing everyone a Happy New Year, while the apparent feud of nothing continues between Ryan and Jon. Ryan brings up the fact that Ray's fancy car got hit while parked outside the studio and then Ryan drops some 411 on the situation across the street involving the person who hit Ray's car and a recent divorce filing. New Years resolutions and a brief "black face" discussion is had leading into the break.After the break, the gang plays a fun little game called Raunchy and Totally F*cked Up Questions. After the rules are explained, the gang jumps into the game and we get to learn quite a bit about Ryan, Dani, Jon, and Ray through the riotous questions and answers. Quite possibly, way more than anyone ever wanted to know.The final segment consists of more hilarious game play that crosses many lines of normalcy and exposes the D2R crew for the Totally F*cked Up and Raunchy group that they might be...but in a good way! Enjoy the depravity.Please SUBSCRIBE to the D2R Podcast on iTunes and don't forget to RATE and REVIEW while you're there.Be a hero - share the D2R Podcast with everyone you know! Your word of mouth is our only advertising and we truly appreciate your support! Thanks for listening and enjoy!!

The majority of neuroendocrine tumors (NETs) of thegastro-enteropancreatic system coexpress sornatostatin receptors (SSTRs)and dopamine type 2 receptors (D2R), thus providing a rationale for theuse of novel SSTR2/D2R chimeric compounds in NET disease. Here weinvestigate the antitumor potential of the SSTR2/D2R chimeric compoundsBIM-23A760 and BIM-23A758 in comparison to the selective SSTR2 agonistBIM-23023 and the selective D2R agonist BIM-53097 on human NET celllines of heterogeneous origin. While having only minor effects on humanpancreatic and bronchus carcinoid cells (BONI and NCI-H727), BIM-23A758induced significant antitumor effects in human midgut carcinoid cells(GOT1). These effects involved apoptosis induction as well as inhibitionof mitogen-activated protein kinase and Akt signaling. Consistent withtheir antitumor response to BIM-23A758, GOT1 cells showed relativelyhigh expression levels of SSTR2 and D2R mRNA. In particular, GOT1 cellshighly express the short transcript variant of D2R. In contrast toBIM-23A758, the SSTR2/D2R chimeric compound BIM-23A760 as well as theindividual SSTR2 and D2R agonistic compounds BIM-23023 and BIM-53097induced no or only minor antitumor responses in the examined NET celllines. Taken together, our findings suggest that the novel SSTR2/D2Rchimeric compound BIM-23A758 might be a promising substance for thetreatment of NETs highly expressing SSTR2 and D2R. In particular, asufficient expression of the short transcript variant of DR2 might playa pivotal role for effective treatment.

Episode 21 has finally arrived after a 2 year delay. I took a good deal of time away from recording my mixes in 2010 and then got back into it in 2011 and to be honest, it took a lot of time for me to get something out there that was not junk. Since this is something that doesn't come to me naturally, it takes a lot of practicing and time for me to crank out something I'm proud of. To give you an idea, I have just under 40 mixes sitting in my documents folder from 2011 and not one of them was what I would consider as worthy for my log of mixes. I have so much music that I want to mix but at the same time realize that it can't all fit into one session. This is the constant conundrum I get in - lots of good tracks individually but collectively junk when I try to get them together. I want to try and get a mix out there at least once a quarter and remind you all that I am still very much into the dance music scene as I have ever been. Hopefully this high energy mix can accompany you for whatever you are out there doing and if nothing else I hope it brings some happiness into your life like it does for me!Tracklist:Cirez D - Mokba (Original Mix)Kyau & Albert - Be There 4 U (Mat Zo Dub)Space Rockerz & Tania Zygar - Puzzle Piece (Daniel Heatcliff's Farewell Remix)Bent - As You Fall (Kyau & Albert Remix)Norin & Rad - Bloom (Original Mix)Tiesto - Everything (Cosmic Gate Remix)Above & Beyond - You Got To Go (Kyau & Albert Remix)Mark Eteson - Jumeirah (Mike Shivers Garden State Remix)Oza - Small Planet (Original Mix)Mat Zo - Synapse Dynamics (Arty Remix)To Listen To This Mix, ClickHERETo Subscribe To This Podcast, ClickHERE

Wow....anyone alive?? I know that this may come as a shock, but I haven't fallen off the end of the earth and I haven't forgotten about electronic music. I have had a very busy second half of 2009, getting into some new hobbies, but still listening to a lot of dance music (surprise, surprise). After dealing with not having Ableton for a while, I have been back at it, digging through my 2009 tracks. I wanted to start the year off with a bang and let everyone know that I will do my best to be more consistent with my music this year and try to release something on a monthly basis (fingers crossed). I am excited to start things off with mainly vocal tracks from 2009 (with one notable exception) that caught my ear. The first track in this mix is probably the most listened to track I have had on my mp3 player for the last half of 2009. I think it is a great way to kick this set off. Please let me know what you think. Here's to another decade...Tracklist:1. Nic Chagall - This Moment (Prog Mix)2. Bellatrax - Can't Hold Back feat. Tina Cousins (Original Extended Mix)3. Ercola - Every Word (Original Mix)4. OceanLab - Lonely Girl (Gareth Emery Remix)5. Tocadisco feat. Nadia Ali - Better Run (Wippenberg Remix)6. Late Night Alumni - You Can Be The One (Sultan & Ned Shepard Remix)7. The Temper Trap - Eurythmic Disposition (Eddie Thoenick Bootleg)8. Eric Prydz - Call On Me (Filterheadz Remix)9. EDX - Shy Shy (Extended Vocal Mix)To Listen To This Mix, ClickHERETo Subscribe To This Podcast, ClickHERE

It has finally arrived.....Episode 3. I put this one together the other night, and it is very housey in a progressive way, it goes up and down in the 5 tracks I mixed here, so check it out and let me know what you think. See you soon :)Tracklist:1. Xplore - Time Travel (Original Mix)2. Nils Noa - Monkeybreak (Inkfish Remix)3. Deadmau5 - Jaded (Original Mix)4. Dale Anderson & Anil Chawla - Panda Love5. Rico Soarez & Andrew Bennett - Precious (Original Mix)To Listen To This Mix, ClickHERETo Subscribe To This Podcast, ClickHERE

So I had a podcast ready to go, and I came across some new music that hasn't been released yet, but will be appearing on Tiesto's big compilation In Search of Sunrise 6. The tracklist recently released and I decided to see what I could dig up on tracks from the album. Tiesto writes “I mixed the album a couple of weeks ago in the old-fashioned way, which means that I didn't use any programs or computerized gadgets for it. I worked with CD-players for the mix. Most of the tracks are so exclusive that, if I'd still be mixing with records, I wouldn't have been able to get those productions on vinyl!” So....exclusive tracks you probably won't hear until Tiesto's album drops....put on your headphones and turn it up. Enjoy :) Tracklist:1. Andy Duguid Feat. Leah - Don't Belong2. The Veil Kings - Searching For Truth (Original Mix)3. Moonbeam - See The Difference (Inside Mix)4. Global Experience - Madras5. Nic Chagall - What You Need (Nic's in Love with Prog Mix)To Listen To This Mix, ClickHERETo Subscribe To This Podcast, ClickHERE

I am really excited to share this mix with you as this is my last mix for 2008. I have probably listened to this one 5 times already on a few different bike rides I have been on alone and I have to say that I am extremely pleased with it. This could quite possibly be one of the more "complete" mixes I have done. It carries a very strong feeling from start to finish and if you listen to it all the way through, I'm sure that you will share this thought. The sounds in this mix are the progressive house sounds that really attract me as a listener and make it a lot of fun to mix a set like this one. This is very different than my trance compilations, so put on your headphones and enjoy the journey. Happy New Year!Tracklist:1. Son Kite - Focus (Shiloh Remix) 2. Ercola - Every Word feat. Daniella (Wendel Kos Club Mix)3. Kismet - Staten Island (Original Mix)4. Nadia Ali - Crash and Burn (Sultan & Ned Shepard Dub Mix)5. Serge Devant - Sweet Harmony (Isma-Ae Remix)6. Jaytech - Vela (Electrobios & Interplay Remix)7. Morgan Page - Call My Name (TV Rock Remix)8. Jay Lumen - Ultra (Original Mix)9. Dinka - Ordinary People (Original Mix)To Listen To This Mix, ClickHERETo Subscribe To This Podcast, ClickHERE

My first shared Ableton Live mix recorded Saturday, August 11, 2007. Let me know what you think, as well as favorite tracks, etc. etc.Tracklist:1. Pryda - Armed2. Deadmau5 & Glenn Morrison - Contact3. Daft Punk - Harder, Better, Faster, Stronger (Deadmau5 Edit)4. Paolo Mojo - Everybody (Original 2006 Mix)5. Cyro - Score A MillionEnjoy!

I put together episode 4 real quickly and it came much more naturally than most of my other sets that I have recorded so far. It is a much more mellow sounding set, but I really enjoy it and look forward to hearing some feedback. Hope all is well :)Tracklist:1. Sebastian Davidson - Gletsjer (Original Mix)2. Sebastian Davidson - Poetic Journey (Original Mix)3. Sebastian Davidson - Nightbird4. Tash & Stage Van H - Detox (Eelke Klejin & Nick Van Hogendoorn Remix)5. M. Paradis - Hello World (Nick Hogendoorn & Eelke Kleijn Remix)To Listen To This Mix, ClickHERETo Subscribe To This Podcast, ClickHERE

Hello there again. I just finished screwing around with some new songs and decided to share this one with you. Its a very mellow progressive house set and one that you can really just zone out to. Check it, let me know what you think :) Hope all is well.Also: The podcast is now updating automatically in iTunes, so if you subscribe in iTunes you won't have to come back to this page again, which is slightly useful.Tracklist:1. Talisman & Hudson - Leaving Planet Earth (Eelke Kleijn Mix)2. Timo Becker - Advantage Becker (Oliver Moldan Remix)3. Roland Klinkenberg & DJ Remy - Mexico Can Wait (Peter Horrevorts Remix)4. Adam K. & Soha - Twilight (Original Mix)5. Deadmau5 - Faxing Berlin (Original Mix)6. Hybrid - Finished Symphony (Deadmau5 Remix)To Listen To This Mix, ClickHERETo Subscribe To This Podcast, ClickHERE

So here's a set to kick off the month of November (even though we are already 11 days in). I appreciate all the feedback on the mixes I have been sharing and even though they may not be in blog response form, I appreciate the phone calls, text messages, facebook posts....etc. Thanks :) I have updated all the past mixes as I realize it was a bit confusing on what links to click as well as what the titles to the tracks I mixed. So all of that is there now and it should be a little easier to navigate through. This mix is one for the club, as I have listened to it a couple of times thinking its a great one to get out on the dance floor too. Again, my progressive house love is continuing to take control of my life, so I hope you are enjoying this one as much as me. I hope all is well with everyone and look forward to chatting soon!Tracklist:1. Cirez D - Horizons (Original Mix)2. Toby Montana & Luetzenkirchen - Last Night In Vegas (Original Mix)3. Noel Sinner - Pull Over (Dabruck & Klein Mix)4. Groove Garcia - Bubblemaker (Original Mix)5. Ercola & Heikki L. - Deep At Night (Adam K. & Soha Remix)To Listen To This Mix, ClickHERETo Subscribe To This Podcast, ClickHERE

November has come and gone quite quickly and there has been a lot of stuff going here with Thanksgiving past and the Christmas season ahead of us. I whipped this mix together kind of spur the moment last night, and decided that this would be one to share to start out here this month. I would like to say there will be a lot of mixes to come this month, but can't promise anything as I'm sure you're all aware how hectic this time of year can get. Anyways, I hope you like this one and are still with my on this progressive house binge. I'm loving it :)Tracklist:1. James Zabiela - Human (Intro Version)2. Glenn Morrison - Circles3. King Unique - Yohkoh (Original Mix)4. Talisman & Hudson - Leaving Planet Earth (Dousk Remix)5. Interplay - Interplay (Original Mix)To Listen To This Mix, ClickHERETo Subscribe To This Podcast, ClickHERE

This compilation has been a long time coming for me, with some big tracks that I have wanted to play for some time. The title of this compilation is Lost In Memory as trance music for me tends to hold specific memories from certain times in my life. I think that this mix also brings with it a sense of being lost, constantly trying to find some sort of equilibrium. For me, the songs seemed to mix together very well, and it came together spur the moment, as most all the mixes I have been proud of do. Anyways, would love to hear what you think of when you listen to this one. Hope 2008 is treating you well so far :)Tracklist:1. Cerf Mitiska and Jaren - Saved Again (Lunacy's Intro Cut)2. Cerf Mitiska and Jaren - Light The Skies (Harry Peat Remix)3. Jody Wisternoff - Cold Drink, Hot Girl (Original Mix)4. Alex Bartlett feat. Anthya - Touch The Sun (Duende Vocal Remix)5. Perasma - Swing 2 Harmony (Original Mix)6. Ferry Corsten - Into The Dark (Ferry Fix)7. 4 Strings - Catch A Fall (Original Mix)8. Phynn - Lucid (Original Mix)9. Vincent De Moor - Fly Away (Cosmic Gate Remix)10. First State feat. Anita Kelsey - Falling (Extended Mix)11. Richard Durand vs. Cassandra Fox - Touch Me vs. Sunhump (Armin Van Buuren Mashup)12. Tiesto - Sweet Things (Tom Cloud Remix)To Listen To This Mix, ClickHERETo Subscribe To This Podcast, ClickHERE

Hello again dear music friends. I have finally brought myself to do an abbreviated version of a trance mix. With a few oldies from 2007 and a few new tunes, I enjoyed bouncing up and down in my room as I listened to/mixed this short set. I hope you feel the same way about this one.Tracklist:1. Sunlounger Feat. Seis Cuerdas - A Balearic Dinner (Club Mix)2. Duderstadt & Kirsty Hawkshaw - Beatitude (Marcus Schössow Dub)3. Cosmic Gate - Always A Fool (Club Mix)4. Marc Marberg with Kyau & Albert - Megashira (Stoneface & Terminal Remix)5. Joonas Hahmo - Sound of Sunday (Original Mix) To Listen To This Mix, ClickHERETo Subscribe To This Podcast, ClickHERE

It's been awhile. I have just made a major transition in my life, moving down to San Diego to take a job with a company called The Active Network. I've been here for 3 weeks, and life has been moving pretty quickly here for the last month, and that is unfortunately why I didn't make a post in March. So, to kick off April, I bring to you my first Electro House Mix, which I really like. Some tunes I have wanted to play for a while, but could never find the right place for them. The track by Funkerman is my current favorite tune, I think the vocals and the buildup make up a classic Electro Sound and its a tune to dance to. Enjoy this mix!Tracklist:1. Dirty South & M.Y.N.C. Project - Everybody Freakin (Original Mix)2. Jay Lumen - Postcard From Alexandria (Original Mix)3. Gold Ryan - Clean Track (Original Mix)4. Funkerman - Speed Up (Original Mix)5. Junkie XL - More (Matthew Dekay Mix)6. Sander Van Doorn - Riff (Original Mix)7. Those Usual Suspects - Greece 2000 (D.O.N.S. & DBN Remix)To Listen To This Mix, ClickHERETo Subscribe To This Podcast, ClickHERE

Spring is here. This mix begins with my favorite intro (which I tend to use in almost all sets that I play live) and morphs into a great trance/electronic dance set. I think that all the tunes in this mix are great tracks for the dance floor. There are very few vocal trance tracks that make me more happy than the 4 included in this set. "Feel Me" could quite possibly be one of the better vocal tracks I've heard in the last year, as it is an extremely uplifting/melodic/beautiful track. I think that this is a great mix to listen to at the end of the day watching the sunset (by the beach for me :P). Hope this mix makes you all as happy as it makes me. Tracklist:1. Deadmau5 - Jaded (Ambient Intro Mix vs. Original Mix)2. Andrea Doria feat. LXR - Beauty of Silence (Inpetto Remix)3. DJ Shog - (Feel Me) Through The Radio (Inpetto Vocal Remix)4. Lange - Songless (Boy Hagemann Remix)5. Tiesto feat. BT - Break My Fall (Adam Kay, Pettigrew & Soha Remix)To Listen To This Mix, ClickHERETo Subscribe To This Podcast, ClickHERE

I took quite a long break since mix 11, so I figured I would post another one here quick after 12. I promised a Trance set and here it is. This one is for all the vocal lovers and trance lovers alike. This set really has the makings of a club mix. These tracks have some amazing drops and great vocals. The synths play a pretty strong role in this set as well. Currently, track number 2 has been going through my head over and over, so I had to throw that one in here. Enjoy!Tracklist:1. Dash Berlin - Till The Sky Falls Down (Vocal Mix)2. Cerf Mitiska and Jaren - You Never Said (Dash Berlin Remix)3. Keane - Is It Any Wonder (Tall Paul Remix)4. Justin Timberlake - Lovestoned (Tiesto Remix)5. Albert Vorne - Formentera What (Gareth Emery Remix)To Listen To This Mix, ClickHERETo Subscribe To This Podcast, ClickHERE

The mix is back! This set is filled with some uptempo trance from start to finish. For all of you that liked my first compilation "Moments of Attraction", this one has some similar sounds. Track 3 is one of my favorites right now as it is an uplifting catchy tune. I hope you all find a nice spot to sit and listen to this mix. Let me know what you think.Tracklist:1. Ernesto V. Bastian - Unchained Melody (Wippenberg Remix)2. The Killers - Read My Mind (Gabriel & Dresden Unplugged Mix) 3. Filterheadz - Day At The Beach (Original Mix)4. Signalrunners - These Shoulders feat. Julie Thompson (Oliver Smith Remix)5. Super8 & Tab - Elektra (Original Mix)To Listen To This Mix, ClickHERETo Subscribe To This Podcast, ClickHERE

Lucky number 15. Being as 15 is my lucky number, I decided to make this mix a special one. A dedication to a producer who I greatly respect and admire, Eric Prydz. He has released so many unbelievable tracks. From mind bending sounds to dance floor hits, this is my attempt to combine some of the various sounds he has through my favorite tracks that he has produced. These tracks sounded the best together, as I tried a few variations of this mix in the last month. I like this one the best and I am also proud of the mash-up that I did with the last track. Sit back, zone out, and let me know what you think.Tracklist:1. Pryda - Armed (Original Mix)2. Pryda - RYMD (Original Mix)3. Pryda - Rage (Original Mix)4. Paolo Mojo - 1983 (Eric Prydz Remix)5. Eric Prydz - Boogie Sound (Original Mix)6. Eric Prydz - F12 (Original Mix)7. Eric Prydz - Pjanno (Original Mix)8. Eric Prydz Vs. Pink Floyd - Proper Education (RDrew DubClub Mashup)To Listen To This Mix, ClickHERETo Subscribe To This Podcast, ClickHERE

Wow....I didn't realize it has been so long since I posted a mix. I knew that I had been putting off a post, but not to this extent. I have been gearing up to race in my 2nd Triathlon this weekend and haven't been doing a whole lot of mixing lately, but am really excited about this mix for a couple of reasons. A good friend of mine just released his first album of electronic tracks! Check out http://www.mattcrofford.com/ to hear his initial release. What is really exciting about this is that I get to mix his music into my sets which is a pretty cool feeling. So, I have included my two favorite tracks off of his new release in this mix. This mix to me is a fun one to listen to and zone out. I'll try and keep it coming with some mixes in the upcoming months, as I have a few Trance mixes that are super high energy and are going to blow some socks off. Enjoy this one!Tracklist:1. Jay Lumen - Calypso (Original Mix)2. Matt Crofford - Eraser (Original Mix)3. Morgan Page Feat. Lissie - The Longest Road (Deadmau5 Remix)4. Matt Crofford - Spectre (Original Mix)5. Deadmau5 - Faxing Berlin (Original Mix)To Listen To This Mix, ClickHERETo Subscribe To This Podcast, ClickHERE

The month of November was pretty busy for me and unfortunately I was unable to mix enough music to find something I really liked. I am (believe it or not) very picky about what I do and do not publish and it truly comes down to whether or not I record enough mixes to be able to pick 1 that I think flows well enough to publish on this podcast. Being as I have only really been mixing music for about 2 years, this process is still sometimes very aggravating, but for the most part is fun to just see what goes well together. Anyways, here is another mix for your listening enjoyment. I decided to go full on trance for this mix, but manage to keep a fairly progressive sound. These tracks are all tracks that I have heard played out by some big names live and they sound really good in clubs and large venues. The Andrew Bennet track, Closer is one of my favorites tunes right now. I took this mix with me on one of my more recent bike rides and decided that it was something that I would share. Let me know what you think.Tracklist:1. Darude - I Ran (So Far Away) feat. Blake Lewis (Gareth Emery Remix)2. Gareth Emery - This Is That (Original Mix)3. Andrew Bennet & DJ Tatana - Closer (Than a Heartbeat) feat. Tiff Lacey (Gareth Emery Remix)4. Mr. Sam - Cygnes feat clAud9 (Original Mix)5. Super8 & Tab - Elektra (Original Mix)To Listen To This Mix, ClickHERETo Subscribe To This Podcast, ClickHERE

Wow. I have been in a major slump. It has been really hard for me to find something that I'm really proud of. That is saying a lot for this past month and a half because I have been cranking away trying to publish something fresh and something with (in my opinion) as much character as my last mix. I think I was so happy with the last mix that everything was a letdown. That's to say until I mixed what you are about to listen to. It's really funny how these things happen, as the minute you just let things flow and stop forcing it, something amazing comes out of it. There are only 2 tracks in this mix with vocals, but I think that they really standout and this for me makes this mix really cool. All of these tracks really bring about a certain mood, and the song that this mix finishes with has been just getting better everytime I listen to it. I can't pinpoint what exactly draws me to the song, but the vocals are definitely apart of it. Jaren's voice is amazing.Tracklist:1. Jay Lumen - Parabola (Original Mix)2. The Jay Lumen Vibe - Atlas (Original Mix)3. Thomas Schwartz - Jupiter Calling (Original Mix)4. Yves Larock - Say Yeah (EDX Re-Dub)5. Chris Lake - Carry Me Away (Original Club Mix)6. Jaytech - Headcase (Original Mix)7. Funkagenda - Breakwater (EDX's Ibiza Sunrise Remix)8. Santiago Nino feat. Jaren - I Don't Know Why (Jerome Isma-Ae Remix)To Listen To This Mix, ClickHERETo Subscribe To This Podcast, ClickHERE

Some great progressive tracks in this one, followed up with one of my favorite Tiesto tracks from In Search Of Sunrise 5. I really enjoy this mix for a couple different reasons. It's a great set to kick-off the spring and I really enjoy the similar sounding progressive tracks in this mix, especially the new track from Joonas Hahmo. With exception to the final track, this set has a load of tracks that mix very well with one another. Give it a listen and let me know what you think! Tracklist:1. Oceanlab - Breaking Ties (Duderstadt Remix)2. Paul Keeley - Life Aquatic (Original Mix)3. Dinka - Asylum (Original Mix)4. Joonas Hahmo - Warm Up (Original Mix)5. Komytea - Professional Killers (Jerome Isma-Ae & Daniel Portman Remix)6. Serge Devant - Addicted (Club Mix)7. Matthew Dekay vs. Proluctors - Let The Game Begin (Original Mix)To Listen To This Mix, ClickHERETo Subscribe To This Podcast, ClickHERE