Podcasts about linkers

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Best podcasts about linkers

Latest podcast episodes about linkers

LiteraturLounge
[Podcast] "Und dann weiß jeder, was ihr getan habt" - Ein tiefer Tauchgang in Christian Linkers Thriller

LiteraturLounge

Play Episode Listen Later Jan 26, 2025 5:48 Transcription Available


In dieser Episode bespreche ich das Buch "Und dann weiß jeder, was ihr getan habt" von Christian Linker. Der Roman beginnt mit der brisanten Situation von Muriel, die eine grausame Theorie über das Verschwinden ihrer besten Freundin Precious hat. Während Muriel versucht, die Wahrheit herauszufinden, lädt sie ihre Mitschüler zu sich nach Hause ein, was eine Kettenreaktion von schockierenden Enthüllungen und Geheimnissen in Gang setzt. Jedes dieser Geheimnisse wird live übertragen und führt dazu, dass die Jugendlichen mit ihren eigenen Ängsten und Schuldgefühlen konfrontiert werden. Ich teile meine persönlichen Eindrücke über die Charaktere und ihre komplexen Beziehungen. Muriel, als einsamer Teenager mit einem emotional distanzierten Vater, und Constantin, der verzweifelt versucht, in die Welt der Reichen und Schönen einzutauchen. Daria, die tiefgläubige Freundin und Lenard, der charmante Tollpatsch, bringen eine weitere Schicht an Dynamik und Konflikten in die Geschichte. Auch Özge, die engagierte Schulsprecherin, spielt eine wichtige Rolle, indem sie sich für ihre Mitschüler einsetzt und versucht, das Gleichgewicht zu wahren. Christian Linker gelingt es, einen fesselnden Thriller zu inszenieren, der nicht nur spannende Unterhaltung bietet, sondern auch gesellschaftliche Themen aufgreift. Ich erörtere, wie der Autor die Herausforderungen von Jugendlichen, Migration und die Schwächen des Gesetzes behandelt. Diese Problemstellungen werden durch die Augen der Charaktere beleuchtet, was dem Leser ermöglicht, sich in ihre Perspektiven hineinzuversetzen. Mit dem Buch gelingt ein Brückenschlag zwischen unterhaltsamer Lektüre und tiefgreifenden sozialen Fragen, die auch für Erwachsene von Interesse sind. Die Art und Weise, wie Linker Themen wie Vorurteile, Freundschaft und Schuld behandelt, macht das Buch zu einer wertvollen Lektüre für Jugendliche und Erwachsene. Die spannende Handlung sorgt dafür, dass man bis zur letzten Seite gefesselt bleibt und bietet alle notwendigen Elemente, um auch für Unterrichtszwecke eine geeignete Wahl zu sein. "Und dann weiß jeder, was ihr getan habt" ist eine packende Lektüre, die sowohl die Jugend als auch das erwachsene Publikum anspricht und einen Einblick in die komplexe Welt der Jugendlichen von heute gibt.

Cents and Sensibility: the Inflation Guy Podcast
Ep. 122: The Role of Linkers in Portfolio Construction, with Chris Clothier

Cents and Sensibility: the Inflation Guy Podcast

Play Episode Listen Later Sep 24, 2024 57:44


The Inflation Guy Podcast welcomes a special guest this week: Chris Clothier, co-CIO of UK asset manager CG Asset Management. Together, Chris and the Inflation Guy discuss how they think about inflation-linked bonds in the context of a portfolio: how to think about their return; different aspects of their risk; and what role/benefit they play in the construction of an 'all-weather' portfolio. Please welcome Chris Clothier to the podcast!   NOTES David Hackett Fischer, The Great Wave: Price Revolutions and the Rhythm of History. (https://www.amazon.com/Great-Wave-Revolutions-Rhythm-History/dp/019512121X ) Blog for this month's CPI: “Inflation Guy's CPI Summary (August 2024)” (https://inflationguy.blog/2024/09/11/inflation-guys-cpi-summary-august-2024/ ) To Subscribe to Quarterly Inflation Outlook: https://inflationguy.blog/shop/    (we may close up subscriptions to this soon) To Subscribe for free to the blog: https://inflationguy.blog/ Check out the website! https://www.EnduringInvestments.com/

The Delhi Public School Podcast
Class 4 - English Ls-4 Grammar (Linkers) | DPS Nacharam - CBSE

The Delhi Public School Podcast

Play Episode Listen Later Sep 18, 2024 3:05


Social Selling Made Simple
How to Supercharge Your Next Marketing Campaign With AI w/Tomer Zuker

Social Selling Made Simple

Play Episode Listen Later Jun 25, 2024 36:45


In today's fast-paced digital world, many agents struggle to create meaningful, personalized connections with their audience. The last thing you want is to come across  as impersonal or fail to resonate on a deeper level.   Believe it or not, AI is one the best ways to build deeper connections. Even though people view it as a cold, impersonal technology, it can allow us to create engaging interactions while saving us money and time.   How can we use it to tailor marketing messages? How do tools like D-ID help us to enhance client experience?   VP Marketing at D-ID, Tomer Zuker, joins me in this episode to discuss the features and benefits of tools such as D-ID, how it can empower us as entrepreneurs and how we can use it to generate an abundance of content to get leads.   AI tools allow us to spare more time in a scalable way to generate more content. -Tomer Zuker   Three Things You'll Learn In This Episode    -D-ID as tech support There's a lot of things that need to be done in our businesses. How can we use tools like D-ID to get those tasks done?   -1 second of video time = 30,000 words. Video content is one of the most efficient ways to market ourselves? What makes it different from written content?   -Training AI in our likeness The more we teach AI, the better the results we get. Can we train AI to adapt our tone and character?    Guest Bio   Tomer Zuker is VP Marketing at D-ID, the leading platform for the generation of Digital Humans. A strategic marketing expert with extensive experience in global growth and go-to-market strategies, he has led marketing initiatives at tech giants like AWS, Microsoft, and IBM. Tomer is also co-founder of the vibrant “Linkers” marketing community on LinkedIn and co-hosts the Market Trip podcast.   Visit https://www.d-id.com/  Find Tomer on LinkedIn @Tomer Zuker Follow Tomer on Instagram @tomer_zuker   Host Bio   ​​Licensed Managing Broker, REALTORS®, avid volunteer, and Major Donor, Marki Lemons-Ryhal is dedicated to all things real estate. With over 25 years of marketing experience, Marki has taught over 250,000 REALTORS® how to earn up to a 2682% return on their marketing dollars.   Six-time REALTOR® Conference and Expo featured attendee, one of 100 speakers selected to speak the REALTOR® Conference & Expo five times, and an Inman closing Keynote Speaker. Marki's expertise has been featured in Forbes, Washington Post, http://Homes.com , and REALTOR® Magazine.

Visión Global
Parte 3: Tertulia monográfica 'digitalización de la hostelería' especial desde FITUR

Visión Global

Play Episode Listen Later Jan 26, 2024 59:58


Edición especial de nuestra tertulia monográfica semana que hacemos desde FITUR 2024. Nos trasladamos a la edición número 44 de la Feria Internacional del Turismo que se está celebrando en Feria de Madrid. Más allá del turismo, venimos a hablar de otro de los temas clave para el tejido empresarial español y es la hostelería, concretamente, hablamos de cómo impulsar a día de hoy mediante la digitalización. Comentaremos la evolución del sector, las herramientas en las que se apoya como las cartas en formato electrónico, pagar la cuenta desde el móvil… Comentamos las estrategias en redes sociales para elevar la presencia de la hostelería. No nos olvidamos de la materia económica, formación y los retos a futuro. Así que en esta tertulia monográfica sobre digitalización de la hostelería nos acompañan David Domínguez como director de Horca Data y digitalización de Hostelería de España, David Basilio, Socio Fundador de Linkers, Albert Justafré, Director de operaciones de GNA Hotel Solutions, Laura Lozano, CEO y cofundadora de Chargy y Jorge Bravo, CEO de Numier.

PaperPlayer biorxiv cell biology
The membrane-actin linkers ezrin, radixin, and moesin are dispensable for macrophage migration and cortex mechanics.

PaperPlayer biorxiv cell biology

Play Episode Listen Later Jul 27, 2023


Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2023.07.27.550674v1?rss=1 Authors: Verdys, P., Rey Barroso, J., Vermeil, J., Bergert, M., Sanchez, T., Metais, A., Mangeat, T., Bellard, E., Bigot, C., Girard, J.-P., Maridonneau-Parini, I., Verollet, C., Lagarrigue, F., Diz-Munoz, A., Heuvingh, J., Piel, M., Du Roure, O., Le Cabec, V., Carreno, S., Poincloux, R. Abstract: The cellular actin cortex provides crucial mechanical support and plays critical roles in numerous functions, including cell division and migration. The proteins of the ERM family, ezrin, radixin, and moesin, are central to these processes by linking the plasma membrane to the actin cytoskeleton. To investigate the individual contributions of these three proteins to leukocyte migration, we generated single and triple ERM knock-out macrophages. Surprisingly, we found that even in the absence of ERMs, macrophages can still form the different actin structures promoting cell migration, such as filopodia, lamellipodia, podosomes, and ruffles. Furthermore we discovered that, unlike every other cell type previously investigated, the single or triple knock-out of ERMs does not affect macrophage migration in a large diversity of contexts. Finally, we demonstrated that the loss of ERMs in macrophages does not affect the mechanics of their actin cortex. These findings challenge the notion that ERMs are universally essential for cortex mechanics and cell migration and support the notion that the macrophage cortex may have diverged from that of other cells to allow for their adaptive cortical plasticity. Copy rights belong to original authors. Visit the link for more info Podcast created by Paper Player, LLC

Inglés en Español
¿Qué es Linkers?

Inglés en Español

Play Episode Listen Later Nov 17, 2022 10:38


Descubre una nueva manera de aprender inglés.

Worldchefs Podcast: World on a Plate
Episode 74: The Power of Exchange in Hospitality Education with David Basilio

Worldchefs Podcast: World on a Plate

Play Episode Listen Later Nov 16, 2022 29:34


On this episode, Ragnar talks with David Basilio of Linkers, Spain's leading hospitality human resources consulting company. Linker has launched an innovative new training program in partnership with Colombia's Ministry of Labor and Universidad Camilo José Cela in Madrid and Toledo. Designed for instructors from public hospitality schools in Colombia, the course offers both gastronomic and business vision, with expert-led classes on gastronomy, technology, and management strategy. Tune in to hear how Linker is fostering intercultural exchange to provide key training across continents and develop the next generation of culinary talent. World on a Plate is supported by Nestlé Professional.

The Grant Williams Podcast
The End Game Ep. 38 - James Aitken PREVIEW

The Grant Williams Podcast

Play Episode Listen Later Oct 30, 2022 12:50


When James Aitken calls and says we need to record another episode of The End Game based on the events of recent weeks, Fleck and I dropped everything and got him on the phone immediately. In this superb conversation, James lays out how and why the turmoil in the UK happened and explains the implications — which extend into the depths of financial market plumbing, an area James knows better than just about anybody. LDIs, Linkers, the box into which the Fed has painted itself and the dreadful predicament facing the BoJ all come under the microscope as James picks apart the plumbing as only he can. Buckle up, folks. Things are about to get really interesting... As a reminder, Silver Tier subscribers to https://www.grant-williams.com get access to both Things That Make You Go Hmmm… and all editions of The Grant Williams Podcast, including The End Game, The Super Terrific Happy Hour, The Narrative Game, This Week In Doom and Shifts Happen so sign up today!

Big Swinging Stocks
$0 - $100k in an Immigrant Family: Linkers Group's Mish and Rubayet on Building Wealth in Low Socio-Economic Environments

Big Swinging Stocks

Play Episode Listen Later Aug 30, 2022 41:44


Sometimes a chance encounter with an investing-savvy builder in a Nissan Skyline GTR can make all the difference… We're back with another episode of $0 - $100k, and this time we're doing something a little different. Deviating from stock market investing, we're talking to brothers in a family owned real estate business, Linkers Group, who made their wealth the hard way in Sydney real estate.  Mish and Rubayet's inspirational story spans Property gains in low socio-economic environments and undesirable suburbs Having to camp on land-lots to secure sales Insecurity driving bad investments Succeeding in a culture that values scholastics despite underachieving in school Wading through the world of FinTok and a rapid-fire Q&A.   Selfwealth Ltd ACN 52 154 324 428 (“Selfwealth”) (Australian Financial Services Licence Number 421789). The information contained on this web site is general in nature and does not take into account your personal situation. You should consider whether the information is appropriate to your needs, and where appropriate, seek professional advice from a financial adviser. Taxation, legal and other matters referred to on this website are of a general nature only and should not be relied upon in place of appropriate professional advice.See omnystudio.com/listener for privacy information.

Ear Catcher
עושים סדר בלינקדאין- ear catcher 255, פרק מיוחד לפסח עם תומר צוקר מייסד-שותף של קהילת Linkers וה-CMO של חברת Kryon

Ear Catcher

Play Episode Listen Later Apr 14, 2022 76:13


עם יד על הלב, איך נראה פרופיל הלינקדאין שלכם? האם הוא מושקע ועשיר בתוכן או בסיסי ומוזנח, כי לא נגעתם בו מאז הפעם ההיא שפתחתם אותו? לכבוד פסח, הזמנתי את תומר צוקר, סמנכ״ל שיווק מנוסה ומוערך, אבל בעיקר אחד האוטוריטות בלינקדאין, כדי לעשות סדר להבין איך נכון וכדאי להתנהל ברשת החברתית הזו, מה חובה לעשות בפרופיל האישי, איך ליהנות מיותר חשיפה, להשיג קשרים מועילים ולקדם את המטרות האישיות והעסקיות. דיברנו הרבה על חשיבות יצירות התוכן בלינקדאין, מה זה ״creator mode״ ולמה כדאי להגדיר את הפרופיל ככזה, על השתתפות בקבוצות, האם כדאי לכתוב בעברית או באנגלית, חשיבות התיוגים וההאשטאגים, איך נכון לפתח את רשת הקשרים, האם כדאי לאשר כל אחד ומהו ה-sales nevigator ובכלל, האם ומתי כדאי לפתוח פרופיל פרמיום בתשלום. פרק חשוב ועמוס בתוכן (כדאי להצטייד במחברת וכלי כתיבה ולכתוב את מה שתומר ממליץ) שיעזור לכם לקדם את המטרות שלכם להשגת עבודה, מיתוג אישי ויצירת לידים ומכירות ללא תשלום. בפרק הבא עם תומר, נדבר על כל אפשרויות הקידום הממומן של לינקדאין ואיך אפשר לנצל את יתרונות הרשת החברתית הזו להשגת מטרות עסקיות. חג שמח. הפרק בשיתוף ״טרגט ספיריט״ המשווקת את הפרסום בתחנות הרדיו של כאן. "דיגיטל אוהב רדיו" ו"רדיו זה כאן" עם מעל 1.8 מיליון מאזינים ביום, בכל הארץ.https://target-spirit.co.il/   ובשיתוף pollit, מערכת סקרים לשימוש עצמי המאפשרת לייצר סקר אונליין תוך מספר דקות ולקבל תוצאות אונליין בזמן אמת ובמחיר אטרקטיבי. http://www.pollit.co.il/   רוצים לראות את הדוגמאות שמדברים עליהן בפרק? כולן מחכות לכם ב-JuiceLetter, הניוזלטר של קבוצת קריאייטיב פירסט. להרשמה לחצו פה: https://www.punkt-adv.com/juiceletter מחפשים עבודה או עובדים? מוזמנים להיכנס ללוח של קבוצת קריאייטיב פירסט, ולפתוח פרופיל או לפרסם מודעה בחינם: .https://creative.cmtz.me/ לתגובות ולשיחות על הפרק ולדיונים והשראה על פרסום, שיווק וקריאייטיב, אתם מוזמנים להצטרף לקבוצת קריאייטיב פירסט: https://www.facebook.com/groups/createam See omnystudio.com/listener for privacy information.

Tagalog With Albine
Subject Markers & Object Markers or Linkers | Tagalog Coaching Session 1

Tagalog With Albine

Play Episode Listen Later Mar 15, 2022 47:38


This is a short lesson on Tagalog Grammar. Specifically, this episode will help you understand how to use the si, ang, ni, ng, sa & kay markers or linkers. Essential Questions: What are the 2 parts of the statement? What are the subject markers in Tagalog language? What are the object markers in Tagalog language? What are the possessive markers in Tagalog language? Subject answers the question what or who. It is the thing or the person that you're talking about. For example, "Si Karen ay aking kaibigan". Sino ang kaibigan? Si Karen. Predicate is the detail or the additional information. Subject & predicate are the 2 parts of the statement. The predicate can be broken down into 3: the verb, the direct object & the indirect object. Notice that: Si & ang are subject markers. Ni & ng are direct object markers. Kay & sa are indirect object markers. --- Send in a voice message: https://anchor.fm/tagalogwithalbine/message Support this podcast: https://anchor.fm/tagalogwithalbine/support

Easy English Podcasts
#26 - Aprenda a CONECTAR suas frases em inglês!

Easy English Podcasts

Play Episode Listen Later Jan 28, 2022 6:22


Hey, there! I hope you enjoy today's episode: Linkers! Follow us on Instagram: @easyenglish.scs

Harvest OPC Sermons
Seekers, Finders, and Linkers

Harvest OPC Sermons

Play Episode Listen Later Jan 23, 2022


PaperPlayer biorxiv biochemistry
Conserved prolines in the coiled coil-OB domain linkers of proteasomal ATPases facilitate eukaryotic proteasome base assembly

PaperPlayer biorxiv biochemistry

Play Episode Listen Later Nov 14, 2020


Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2020.11.13.381962v1?rss=1 Authors: Cheng, C. L., Wong, M. K., Li, Y., Hochstrasser, M. Abstract: The proteasome is a large protease complex that degrades both misfolded and regulatory proteins. In eukaryotes, the 26S proteasome contains six different AAA+ ATPase subunits, Rpt1-Rpt6, which form a hexameric ring as part of the base subcomplex that drives unfolding and translocation of substrates into the proteasome core. Archaeal proteasomes contain only a single type of ATPase subunit, the proteasome-activating nucleotidase (PAN), which forms a trimer-of-dimers and is homologous to the eukaryotic Rpt subunits. A key PAN proline residue (P91) forms cis and trans peptide bonds in successive subunits around the ring, allowing efficient dimerization through upstream coiled coils. The importance of the equivalent Rpt prolines in eukaryotic proteasome assembly was unknown. We show an equivalent proline is strictly conserved in Rpt3 (in S. cerevisiae, P93) and Rpt5 (P76), well conserved in Rpt2 (P103), and loosely conserved in Rpt1 (P96) in deeply divergent eukaryotes, but in no case is its mutation strongly deleterious to yeast growth. However, the rpt2-P103A, rpt3-P93A, and rpt5-P76A mutations all cause synthetic defects with specific base assembly chaperone deletions. The Rpt5-P76A mutation decreases the levels of the protein and induces a mild proteasome assembly defect. The yeast rpt2-P103A rpt5-P76A double mutant has strong growth defects attributable to defects in proteasome base formation. Several Rpt subunits in this mutant form aggregates that are cleared, at least in part, by the Hsp42-mediated protein quality control (PQC) machinery. We propose that the conserved Rpt linker prolines promote efficient 26S proteasome base assembly by facilitating specific ATPase heterodimerization. Copy rights belong to original authors. Visit the link for more info

PaperPlayer biorxiv biophysics
Influence of membrane-cortex linkers on the extrusion of membrane tubes

PaperPlayer biorxiv biophysics

Play Episode Listen Later Jul 28, 2020


Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2020.07.28.224741v1?rss=1 Authors: Paraschiv, A., Lagny, T., Coudrier, E., Bassereau, P., Saric, A. Abstract: The cell membrane is an inhomogeneous system composed of phospholipids, sterols and proteins that can be directly attached to underlying cytoskeleton. The linkers between the membrane and the cytoskeleton are believed to have a profound effect on the mechanical properties of the cell membrane and its ability to reshape. Here we investigate the role of membrane-cortex linkers on the extrusion of membrane tubes using computer simulations and experiments. In simulations we find that the force for tube extrusion has a non-linear dependence on the density of membrane-cortex attachments: at a wide range of low and intermediate densities of linkers the force is not significantly influenced by the presence of membrane linking proteins and resembles that of the bare membrane. For large concentrations of linkers however the force substantially increases compared to the bare membrane. In both cases the linkers provided membrane tubes with increased stability against coalescence. We then pulled tubes from HEK cells using optical-tweezers for varying expression levels of the membrane-cortex attachment protein Ezrin. In line with simulations, we observed that overexpression of Ezrin led to an increased extrusion force, while Ezrin depletion had negligible effect on the force. Our results shed new light on the importance of local effects in membrane reshaping at the nanoscopic scales. Copy rights belong to original authors. Visit the link for more info

Capital Radio - On Demand
Especial desempleo y coronavirus: los hoteles

Capital Radio - On Demand

Play Episode Listen Later Apr 2, 2020 7:06


Hablamos sobre cómo está afectando al sector turístico y a los hoteles esta crisis, que se ha cebado en el sector servicios, con David Basilio, CEO de Hosteleo y director de operaciones de Linkers

L.A.W. Talk with KrysSpeaks
We Are Better Together

L.A.W. Talk with KrysSpeaks

Play Episode Listen Later Nov 13, 2019 7:05


Linkers share their experience about The 2019 L.A.W. Gathering. To join us for next year’s gathering, Subscribe Today to linkingallwomen.com for news and updates.

Caribbean Radio Show Crs Radio
Farewell Tribute to Winston Watson, an original member of the The Meditations

Caribbean Radio Show Crs Radio

Play Episode Listen Later Mar 30, 2019 74:00


The Meditations are a reggae vocal harmony group from Jamaica formed in late 1974. They have released several studio albums and are still performing in the 2000s and today. The Meditations were formed in late 1974, when Danny Clarke left The Righteous Flames, recruiting Ansel Cridland (previously of The Linkers) and Winston Watson After releasing singles credited to the individual members, they began recording as The Meditations in late 1976, shortly after which they released their biggest hit, "Woman Is Like a Shadow", which sold over 45,000 copies in its first month of release. They recorded in the mid-1970s for producers such as Dobby Dobson, Joseph Hoo Kim, and Lee "Scratch" Perry, their righteously Rastafarian style gaining comparisons with The Mighty Diamonds. Their first album, Message From The Meditations, was released in 1977. Robert Christgau called it "a nice one" in Christgau's Record Guide (1981), highlighting the "island chauvinism" of songs like "Running from Jamaica", which "gets on those who emigrate to Canada, Britain, the States, and Africa #nowplaying

TALENTO HUMANO
Talento Humano 16. Con Sandra Granell de GT Linkers.

TALENTO HUMANO

Play Episode Listen Later Oct 22, 2018 21:40


En el episodio nº 16 de Talento Humano entrevistamos ahttp://gtlinkers.com/ Sandra Granell de la empresa GT LINKERS. Una consultora intertnacional de Recursos Humanos que pone en contacto talentos y empresas de manera global (internacional). Si eres un profesional en búsqueda de nuevas oportunidades, o una empresa en busca del talento ideal, ponte a escuchar ya mismo este podcast. Escúchalo desde tu reproductor de podcast o desde la pagina web de Clikdeo. Plataforma de RRHH para cazatalentos y headhunters.

The Outer Sanctum
We Are Linkers

The Outer Sanctum

Play Episode Listen Later Sep 21, 2017 49:30


Politics in sport, finals mania and inside the mind of a coach. See acast.com/privacy for privacy and opt-out information.

politics linkers
The Outer Sanctum
We Are Linkers

The Outer Sanctum

Play Episode Listen Later Sep 21, 2017 49:30


Politics in sport, finals mania and inside the mind of a coach.  

BSD Now
207: Bridge over the river Cam

BSD Now

Play Episode Listen Later Aug 16, 2017 103:11


We recap our devsummit experiences at BSDCambridge, share why memcmp is more complicated than expected, explore Docker on FreeBSD, and we look at a retro terminal. This episode was brought to you by Headlines BSDCam recap (https://wiki.freebsd.org/DevSummit/201708) The 2017 Cambridge DevSummit took place from 2-4 August 2017. The event took place over three days including a formal dinner at St John's College, and was attended by 55 registered developers and guests. Prior to the start of the conference, we had a doc hacking lounge, the computer lab provided a room where we could meet and try to spend some time on documentation. Sevan walked two interested people through the process of creating a documentation patch and submitting it for the first time. In the process, found ways to improve the documentation on how to write documentation. The event is run "un-conference style" in that we brainstorm the actual session schedule on the first morning, with a focus on interactive topics that reflect the interests and exploit the knowledge of the attendees. The idea is to maximize the amount of discussion and decisions that can be made while we are all in the same room The first morning, we all gather in the slightly too small, and even more slightly under air conditioned FW11 classroom. We go around the room introducing ourselves, and listing a few topics we would be interested in discussing. Eventually the whiteboard is full of topics, with various numbers of ticks beside them to indicate the number of interested people There are breakout rooms of all sizes, so even topics with only a small group of interested folks can get a lot accomplished The most difficult is trying to schedule the sessions, as there is much overlap and people usually want to be in concurrent sessions, or someone's schedule means they won't be available that day, etc. This years working groups: Toolchain (Compilers, Linkers, External Toolchain, Static analysis and sanitizers) Virtualization (bhyve, xen, jails, docker) Transport (TCP) and Network Performance Security and mitigations (W^X, noexec stack, CFI, ASLR, KASLR, Safe Stack, etc) Testing (Status, What to test, How to test, QA for releases) Capsicum (Automation with LLVM etc, Casper, Namespacing, “Services”, capsh) Desktop / WiFi (drm-next, drivers, resume, power, installer, desktop, OOB Experience) Tracing (Blackbox, DTrace, KTR, ptrace, truss, hardware tracing) Packaging and Packaged Base (Sets, Kernels, Ports & flavours, sub-packages, privlib) Architectural Security Features (CPU Features: SGX, PXN/PAN, Pointer Authentication, AMD Memory Encryption, Libcrunch, RISC-V, CheriABI) Architectures and Embedded systems (RISC-V, ARM, ARM64, MIPS(64), SPARC64) Teaching (Audiences, Objectives, Targets, Material, future directions) Provisioning and Management Tools (CfgMgmt tools, Image building, VM/bhyve orchestration, Preconfigured VMs for testing, Wishlist) Storage (ZFS status update, ZFS encryption infrastructure, ZFS Zero Copy / Sendfile, Acceleration of checksums and raidz parity calculations, sesutil, mpsutil) And that wasn't everything. We then had a series of short talklets: Enhancing and replacing mmap() SDIO support eBPF support for FreeBSD Tracing + Virtualization Practical DMA Attack Protection On Thursday night there was a special dinner at St John's College Overall it was a great DevSummit, and I even managed to get some of the work assigned to me finished. Shortly I will commit an update to the boot loader menu that will automatically populate the kernel selection menu with the automatically detected list of installed kernels. The list is also properly refreshed when you switch boot environments. *** Hosts/BSD – for when you need to run your BSD inside a penguin (https://wiki.qemu.org/index.php/Hosts/BSD) This wiki provides details on how to run each of the various BSDs under QEMU The target audience is Linux developers looking to test their apps etc under BSD The wiki is in need of some love, there are some option questions, and it lacks some polish There are instructions on building qemu from source, but it should likely mention the qemu-devel port There should probably also be instructions on using other architectures, like ARM/MIPS etc If you have used QEMU, or would like to spend the time to learn how, please help update this wiki *** memcmp -- more complicated than you might expect (http://trust-in-soft.com/memcmp-requires-pointers-to-fully-valid-buffers/) “A suspicious pattern in open-source software” One bug recently found by John using tis-interpreter on a widely used open-source library involved the comparison of strings with memcmp. The unexpected condition was that memcmp was, in one case, called with a pointer to a buffer shorter than the length passed as third argument, breaking one of the two symmetrical pre-conditions in the function's ACSL contract A reason that may have made this use of memcmp look okay to the developer is that the buffers being passed to it always differed before the end of the buffers were reached. a memcmp implementation based on stopping as soon as a difference is found, would not have caused any out-of-bounds read access The first question raised was whether the pattern memcmp("a", "bc", 3) was problematic according to the letter of the C standard. If it was, the second question was whether the busy maintainer of one of the Open Source packages that make the Internet tick should be bothered with a bug report. I would like to be able to say that memcmp's ACSL contract was the product of careful deliberation, but unfortunately this is not the case: many standard function contracts were written quickly in order to get most of the standard library covered, and have not been tested by time. Anyway, upon proofreading the relevant clause in the C11 standard, my feeling was that the ACSL formalization was, in this particular case, right, and that it was undefined behavior to pass as memcmp argument a buffer that wasn't fully valid, even if the implementation sort-of needs to read the buffer's characters in order for the purpose of finding the first mismatch. The post then goes on to look at the memcmp code in glibc There are two distinct optimizations for long buffers, one that applies when both buffers start at the same offset modulo the word size, memcmpcommonalignment, and one that applies when they don't, memcmpnotcommonalignment. The function memcmpcommonalignment is relatively well-behaved: it reads from the two buffers aligned word by aligned word, and thus reads the entire words that contain differing bytes. If the caller passed buffers that aren't valid after the differing byte, this amounts to reading out of bounds, but this sort of out-of-bounds access is not detected by the typical MMU, which works at the scale of the page. The “notcommon_alignment” case, however, tells a different story. When passed the carefully (mis-)aligned buffers t1 and (char*)t2+1, although these buffers differ in the 8th byte, Glibc's implementation of memcmp reads 8 bytes beyond the end of t1. By making the 16th byte differ instead of the 8th one, it is also possible to make Glibc's implementation of memcmp read 16 bytes beyond the end of t1. In conclusion, yes, some implementations of memcmp will crash when invoked with buffers that aren't valid for the full length, even if they differ early. The circumstances are rare (probably the reason this bug was still there to be found in a library that had already been tested with all the available techniques) but outside the programmer's control. The pattern described in this post should be reported as a bug when found. It is interesting to read the detailed analysis of a bug in such a basic libc feature *** News Roundup Docker on FreeBSD (http://daemon-notes.com/articles/network/docker) There are two approaches to running Docker on FreeBSD. First one was created back in 2015 and it was a native port of Docker engine to FreeBSD. It was an ambitious project but nobody stepped forward to continuously port the never-ending flow of upstream code to FreeBSD. So the port still exists (sysutils/docker-freebsd) but it wasn't updated since 2015 and it is Docker v1 (it is v17 as of 2017). The other approach is to use official way of running Docker on platforms other than Linux. Well, somewhat official as Docker still does not support FreeBSD as a host officially. This is docker-machine tool which in turn will use VirtualBox to run a virtual machine with Linux and Docker engine. docker utility on the host will communicate with the engine inside VB where all the work will be done. This article describes what needs to be done to start using it. Before we begin you need VirtualBox installed. Do not skip adding /boot/loader.conf and /etc/rc.conf lines mentioned on that page. You won't need user inteface or anything, docker-machine will do all the work, just make sure VirtualBox is present and ready to be used. `pkg install docker docker-machine docker-compose' Docker will store its stuff in ~/.docker. You might not want the virtual machine image files to live in your home, in this case just create a symlink: mkdir ~/.docker ln -s /storage/docker ~/.docker/machine docker-machine create --driver virtualbox --virtualbox-memory 2048 --virtualbox-cpu-count 2 --virtualbox-disk-size 102400 --virtualbox-hostonly-cidr "10.2.1.1/24" docker1 Here's the example. We are creating machine named docker1. It is using VirtualBox driver, the vm has 2G of memory, 2 cores and 100G of disk space. docker-machine setups VirtualBox to use host-only network adapter (it will create vboxnet0 interface on the host automatically) and we are instructing it to use 10.2.1.1/24 as the address of this adapter — change it to what suits your needs or omit this flag (default is 192.168.99.1/24). And basically that is all. Check if it is running: docker-machine ls If you do open VirtualBox interface you will find a virtual machine named docker1 running. You can start/stop/whatever your machine using docker-machine utility. Here's how you can connect to the machine: docker utility by default tries to talk to Docker engine running on the same host. However with specific environment variables you can instruct it to talk to other host. docker-machine can export these variables for you. eval docker-machine env docker1 docker run hello-world There was quite a bit of discussion about docker at the FreeBSD developers summit in Cambridge during the first week of August. Two docker developers who had worked on the Mac OS X port, one of whom is an OpenBSD advocate, explained how docker has evolved, and the linux-isms have been abstracted away such that a truly native docker solution for FreeBSD can be built and maintained with a lot less headache than before I look forward to seeing if we can't make that happen *** The POSIX Shell And Utilities (http://shellhaters.org/) The POSIX Shell And Utilities Compiled for The Shell Hater's Handbook *** PostgreSQL – logging to a file (http://dan.langille.org/2017/07/31/postgresql-logging-to-a-file/) These steps were carried out on FreeBSD 11.0 with PostgreSQL 9.6 (two of my favorite tools). I like logging. I like logging PostgreSQL. With logs, you can see what happened. Without, you can only guess. Setting up logging for PostgreSQL involves several parts, each of which must be completed or else I don't get what I want. This is not a criticism of PostgreSQL. It's a feature. I am documenting this because each time I configure a new PostgreSQL instance, it takes me more than one iteration to get it working. The goal: this post lets both you and me get it right the first time. The parts include: + Telling PostgreSQL to log via syslog + Telling FreeBSD to local postgres to /var/log/postgres.log (my preference). + Telling PostgreSQL the things you want logged. + Changes to postgresql.conf The file location varies with the version installed. For PostgreSQL 9.6 on FreeBSD, the file is /var/db/postgres/data96/postgresql.conf (adjust 96 according to the version installed). I made these changes to that file. log_destination = 'syslog' log_min_messages = notice log_min_error_statement = notice log_checkpoints = on log_lock_waits = on log_timezone = 'UTC' By default, PostgreSQL logs to the local0 facility and is controlled by the syslog_facility in postgresql.conf. This will be used in syslog.conf (see the next section of this post). The above mentioned changes require a reload: service postgresql reload Changes to /etc/syslog.conf Now that we have PostgreSQL logging to syslog, we want to tell syslog where to put those messages. I changed this line in /etc/syslog.conf:*.notice;authpriv.none;kern.debug;lpr.info;mail.crit;news.err /var/log/messages With .notice pulling in some local0 messages, adding local0.none to the line will free the messages up for later use in the configuration file. Otherwise, the PostgreSQL messages will be in /var/log/messages. The changed line is: `.notice;authpriv.none;kern.debug;lpr.info;mail.crit;news.err;local0.none /var/log/messages Then, to get the messages into my preferred location, I added this to the file: local0.* /var/log/postgresql.log` Log file rotation For rotating my log file, I added a new file: /usr/local/etc/newsyslog.conf.d/postgresql96 /var/log/postgresql.log pgsql:wheel 640 7 * $D0 GB /var/db/postgres/data96/postmaster.pid 30 Before restarting syslog, I did this, so the destination file existed. This isn't always/strictly necessary, but because the ownership is not chown root:wheel, I do it to get that part set. touch /var/log/postgresql.log chown pgsql:wheel Restarting syslog: sudo kill -HUP `sudo cat /var/run/syslog.pid ` That's it Now you should see PostgreSQL logging in /var/log/postgresql.log. mandoc-1.14.2 released (http://undeadly.org/cgi?action=article&sid=20170729122350) i just released portable mandoc-1.14.2. It is available now from http://mandoc.bsd.lv/ (http://mandoc.bsd.lv/). ```From: Ingo Schwarze schwarze@usta.de Date: Fri, 28 Jul 2017 20:12:44 +0200 To: discuss@mandoc.bsd.lv Subject: mandoc-1.14.2 released Hi, i just released portable mandoc-1.14.2. It is available now from http://mandoc.bsd.lv/ . All downstream maintainers are encouraged to update their ports and packages from 1.14.1 to 1.14.2. Mandoc 1.14.2 is a feature release introducing: a new -Tmarkdown output mode anchors for deep linking into -Thtml manual pages a superset of the functionality of the former mdoclint(1) utility a new -Wstyle message level with several new messages automatic line breaking inside individual tbl(7) cells a rewrite of the eqn(7) lexer, and some eqn(7) rendering improvements support for many additional low-level roff(7) features and various smaller features and bug fixes. For more details, see: http://mandoc.bsd.lv/NEWS With the improved mandoc features, only twenty-five out of the ten thousand software packages in the OpenBSD ports tree still need groff to format their manual pages. Since the project has been called "mandoc" rather than "mdocml" for several years now, the website, the distribution tarball, and the source extraction directory are now also called "mandoc" rather than "mdocml". The release was tested on the following systems: + OpenBSD-current and OpenBSD-stable + NetBSD-current + illumos + Debian Linux + Void Linux x86_64 glibc and musl + Crux Linux + SunOS 5.11.2, 5.10, and 5.9 As before, catman(8) and the regression suite cannot be used on SunOS 5.10 and SunOS 5.9. A big thanks to everybody who provided patches, bug reports, feature suggestions, advice, and help with testing! Yours, Ingo``` Beastie Bits A good looking terminal emulator which mimics the old cathode display. Available in x11/cool-retro-terminal (https://github.com/Swordfish90/cool-retro-term) Milestone Complete! OpenRC conversion (https://www.trueos.org/blog/milestone-complete-openrc-conversion/) Healthy developer interaction between FreeBSD and IllumOS re: mdb (https://illumos.topicbox.com/groups/developer/discussions/T5eae6079331c4df4) Large Batch of Kernel Errata Patches Released (http://undeadly.org/cgi?action=article&sid=20170804053102) opnsense 17.7 released (https://opnsense.org/opnsense-17-7-released/) Twitter Co-Founder and CEO states “FreeBSD rules them all” (https://twitter.com/jack/status/892605692317650944) Hurry up and register for vBSDCon September 7-9 (http://www.verisign.com/en_US/internet-technology-news/verisign-events/vbsdcon/index.xhtml?dmn=vBSDcon.com) and EuroBSDCon September 21-24 (https://2017.eurobsdcon.org/) *** Feedback/Questions Dominik - Monitoring Software (http://dpaste.com/08971FQ) Darren - Wonderful Awk (http://dpaste.com/0YCS4DN) Andrew - Thanks (http://dpaste.com/0ZREKTV) Jens - Migration Questions (http://dpaste.com/1GVZNWN) ***

Aprende ingles con inglespodcast de La Mansión del Inglés-Learn English Free
Adverbial clauses, linkers and conjunctions - AIRC133

Aprende ingles con inglespodcast de La Mansión del Inglés-Learn English Free

Play Episode Listen Later Dec 11, 2016 48:47


Adverbial clauses, linkers and conjunctions - AIRC133 Más podcasts para mejorar tu ingles en: http://www.inglespodcast.com/  More podcasts to improve your English at: http://www.inglespodcast.com/  In this episode we're going to help you with Adverbial clauses, linkers, conjunctions etc Audio feedback from Edu from Peru You can sign up for free English chat at inglespodcast.com We also publish the dates and the times of the online conversaiton chat on our Facebook page (https://www.facebook.com/mansioningles/) and on Twitter (https://twitter.com/ - @mansiontwit) Listener Feedback: Bruno Schvidah from Brazil sent us an email. My name is Bruno and I am from Brazil but recently living in Copenhagen! My weak side has been "adverbial clauses and linkers" I would really like to go through that! For now, I wish you all a great Friday! All the best, Bruno We're sorry it's taken us so long to answer your question, Bruno. We spoke about some linking words in Episode 55 (but, even though/although, however, in spite of/despite) ( http://www.inglespodcast.com/2015/06/14/linking-words-but-even-thoughalthough-however-in-spite-ofdespite-etc-shopping-expressions-airc55/ ) What Are Adverbial Clauses? "An adverbial clause is a group of words which does what an adverb does. Adverbial clauses (like all clauses) contain a subject and a verb. For example: "I eat dark chocolate daily." (normal adverb) "I'm going to eat dark chocolate until you tell me to stop." (adverbial clause = "until you tell me to stop") More examples: I never knew how wonderful life could be until I started podcasting. I'll let you know as soon as I publish this episode. Now that we've eaten, we can have some of that chocolate cake. Adverbial clauses don’t have to speak about time. They can also be about contrast, cause and effect, condition etc. Contrast:I had some chocolate cake even though I was full. (even though = aunque) Cause and effect: I’ve put on weight this month because I’ve been eating so much cake. Condition: I’m not going to Disneyland unless you come with me. (unless = a menos que, a no ser que) More linkers: In spite of / Despite - “I bought an iphone in spite of the price.” (in spite of/despite = a pesar de) They go at the beginning or in the middle of a sentence. “Reza arrived on time despite / in spite of missing the bus” Instead of (en vez de, en lugar de) “This year we’re having roast lamb for Christmas dinner instead of turkey.” Although (aunque) / though / even though / in spite of the fact that (a pesar de que) - “Although/Even though/Though I was full, I had another piece of cake.” "Even though" is more emphatic than "although". “Though” can also go at the end of a second sentence in informal English: These connectors are followed by a complete sentence. They can be placed at the beginning or in the middle of the sentence. If they are at the beginning we put a comma after the clause. "I had another piece of cake, although I was full." “In spite of the fact that the neighbours were making a noise, we decided to record this podcast.” “Reza loves Berta. She rarely thinks about him, though.” "I'm sorry, we don't have any chocolate cake. We've got some biscuits, though." However (sin embargo) / nevertheless/nonetheless (no obstante) / even so (aun así) At/Near the start of a second sentence: “I was really tired. Even so, I decided to go out for a beer.” (sin embargo, aun así) “Craig’s on a diet. However, he can’t lose any weight.” “I didn’t like the price. Nevertheless/Nonetheless, I bought it.” Or at the end of a second sentence in informal English: “I didn’t like the price. I bought it, nonetheless.” While / whereas (mientras que) In the middle or at the start of a sentence: “Our last podcast was really interesting, whereas/while this one is a bit boring.” “While/whereas the last podcast was really interesting, this one is a bit boring:” On the one hand / on the other hand (por un lado / por el otro lado) Links two contrasting ideas. “On the one hand” can be omitted: (On the one hand,) I think that technology has helped society in the areas of health, work, education etc. On the other hand we might be too dependant on technology and maybe to addicted to it as well. On the contrary - al contrario Some people say that people can’t change. On the contrary, I think they can! We can use linkers to add information: Moreover (además) / furthermore / besides (además) / in addition (to) (además (de)) / as well as (además de) / apart from (aparte de) / what’s more (además; lo que es más, y encima) / on top of that (además) / as well = too (también) at the end of a sentence “In addition to jazz music, Craig also likes rap.” Besides music and podcasting, what other hobbies do you have at the moment? - It's a nice day for a walk, and besides, I need the exercise. I think you owe me an apology. Furthermore, you need to apologise to my wife. Your company did not inform us of the building work in the hotel. Moreover, no compensation was offered. Juan was at the meeting, as well as Sara and Maria. ‘Apart from Spanish, Reza also speaks French”. “He's ugly and what's more, he's not very nice.” "What a day! First I woke up late, then the car wouldn't start, and on top of that, I dropped my phone and broke it." She likes tea. She likes coffee as well/too. We can use linkers to show consequences and results: As a result (of) (debido a,como resultado, como consecuencia) / therefore (por lo tanto, por eso) / consequently, as a consequence (en consecuencia, y entonces, y por eso) / for this reason “Reza and I work very hard on this podcast. As a result, it’s becoming one of the most popular learning English podcasts in itunes.” “I think, therefore I am.” - pienso, luego existo “I wanted it; consequently, I bought it.” “I don’t think I can help you develop this product, and for this reason I’m out.” - Shark tank We can also use linkers to show reasons and causes: Because (of) (a causa de, debido a) / as / since / seeing that / on account of / due to (debido a) / due to the fact that (debido a que) / owing to / owing to the fact that As/Since/Because Craig loves Mickey Mouse (clause), he wants to visit Disney Land, Orlando. Because of Craig’s love for Mickey Mouse (noun phrase, not clause), he wants to visit Disney Land, Orlando. “Because of / on account of / owing to / due to our sponsor, italki we are able to continue with this podcast.” Compare: “We didn’t record podcasts yesterday due to* work.” “We didn’t record podcasts yesterday due to the fact that* we were both working.” (*also owing to and owing to the fact that) “As / Since / Seeing that we’re hungry, we should break for lunch now.” In order to = a more formal version of INFINITIVE / so as to Theses conjunctions explain the purpose of something. They are more common in written English. Reza and I started this podcast (in order) to help you improve your English. We started our Patreon program so as to be able to pay for full transcriptions for these podcasts (go to https://www.patreon.com/inglespodcast for more details) All the same (de todas formas, a pesar de todo) “They offered to pay me 30 euros per hour, but I turned down the job all the same.” We can use some linking words to show the order and sequence of things First of all / Firstly / To begin with / To start with / In the first place (en primer lugar, antes que nada) Second / Secondly (en segundo lugar) Third / Thirdly (en tercer lugar) After that / Then (después de eso, después) / Next (luego) Finally, Lastly (por fin, por último, finalmente) Last but not least (por último, si bien no menos importante) Linkers for summarizing In short / To sum up / In conclusion (en resumen, para concluir, resumir) All in all (en suma) In brief (en resumen) In short (en resumen) On the whole (en general) ...and now it's your turn to practise your English. Do you have a question for us or an idea for a future episode? Send us a voice message and tell us what you think. https://www.speakpipe.com/inglespodcast Nuestra tienda de descargas: http://store.mansioningles.net/ Send us an email with a comment or question to craig@inglespodcast.com or belfastreza@gmail.com. Thanks to our Patreon sponsors who are: Lara Arlem Carlos Garrido Zara Heath Picazo Mamen Juan Leyva Galera Sara Jarabo Corey Fineran from Ivy Envy Podcast Manuel García Betegón Jorge Jiménez Raul Lopez Rafael Daniel Contreras Aladro Manuel Tarazona New sponsor: Mariel Riedemann On next week's episode: Feelings Vocabulary in English Más podcasts para mejorar tu ingles en: http://www.inglespodcast.com/  More podcasts to improve your English at: http://www.inglespodcast.com/  The music in this podcast is by Pitx. The track is called 'See You Later'        

Fakultät für Biologie - Digitale Hochschulschriften der LMU - Teil 05/06
Strukturelle und funktionale Analyse der Effektordomäne des pH-abhängigen Einkomponentensystems CadC in Escherichia coli

Fakultät für Biologie - Digitale Hochschulschriften der LMU - Teil 05/06

Play Episode Listen Later May 6, 2015


Das Einkomponentensystem CadC in Escherichia coli zählt zur Gruppe der ToxR-ähnlichen Transkriptionsregulatoren und aktiviert bei niedrigem pH-Wert die Expression des cadBA-Operons, einem Säure-induzierbaren Lysin-Decarboxylase-System. Transkriptionsregulatoren der ToxR-Familie zeichnen sich durch einen gemeinsamen modularen Aufbau aus und bestehen aus einer periplasmatischen Sensordomäne, einer Transmembranhelix und einer zytoplasmatischen Effektordomäne. Die Signalwahrnehmung, -weiterleitung und -verarbeitung erfolgt bei den ToxR-ähnlichen Transkriptionsregulatoren innerhalb eines einzelnen Proteins. Die molekularen Mechanismen der Reizwahrnehmung durch CadC sind bekannt, die Signalweiterleitung und -verarbeitung im Zytoplasma sind hingegen weitgehend ungeklärt. In CadC ist ein zytoplasmatischer Linker (51 Aminosäuren) essentiell für die Signaltransduktion von der sensorischen Domäne zur DNA-Bindedomäne. Im ersten Teil dieser Arbeit wurde der Mechanismus der Signalweiterleitung von der sensorischen Domäne zur DNA-Bindedomäne untersucht. Mit Hilfe der Kernspinresonanzspektroskopie konnte gezeigt werden, dass die Linkerregion unstrukturiert vorliegt. Im Rahmen einer umfangreichen Mutagenesestudie wurde beobachtet, dass sowohl eine Vielzahl an Aminosäuresubstitutionen (Veränderungen der Ladung, der Rigidität oder der Wahrscheinlichkeit zur Bildung einer α-Helix) als auch die Verlängerung des CadC-Linkers zu keiner funktionellen Beeinträchtigung führte. Jedoch wurde die Signalverarbeitung im Zytoplasma durch Verkürzung des Linkers modifiziert und verursachte ein invertiertes Expressionsprofil des Zieloperons cadBA oder die Entkopplung der Expression vom externen pH. Der Linkerregion in CadC konnte keine Rolle in der Oligomerisierung zugeordnet werden. Unabhängig vom Linker wurde in einer in vivo Interaktionsstudie eine pH-abhängige Interaktion (pH < 6,8) zwischen CadC-Monomeren gezeigt. Im zweiten Teil dieser Arbeit wurde die Röntgenkristallstruktur (2,0 Ångström) und in einem parallelen Ansatz die NMR-Struktur (0,46 backbone RMSD) der zytoplasmatischen Effektordomäne in CadC als erste dreidimensionale Struktur der DNA-Bindedomäne eines ToxR-ähnlichen Regulators aufgeklärt. In der Struktur von CadC1-107 wurde ein „winged Helix-Turn-Helix“-Motiv aus der Familie der OmpR-ähnlichen Transkriptionsregulatoren beobachtet. Im Gegensatz zu der Topologie bereits gelöster OmpR-ähnlichen Regulatoren enthält CadC am Übergang von DNA-Bindedomäne und Linkerregion einen zusätzlichen β-Strang (β-Strang 7), welcher sich stabilisierend auf die DNA-Bindung auswirken könnte. Im dritten Teil dieser Arbeit wurde der DNA-Bindemechanismus von CadC an den cadBA-Promotor untersucht. In in vitro Versuchen zur Bindung von löslichen CadC-Varianten an DNA konnte eine sehr geringe Dissoziationsrate beobachtet werden. Somit ist nicht die Affinität zur DNA sondern die Stimulus-abhängige Interaktion von CadC mit der α-Untereinheit der RNA-Polymerase essentiell für die Aktivierung des cadBA-Operons. Außerdem wurden, basierend auf der Kristallstruktur der DNA-Bindedomäne von CadC Aminosäuresubstitutionen durchgeführt. Die Aminosäure His66 in der Erkennungshelix α3 ist an der Interaktion mit der großen Furche der DNA beteiligt, während die Aminosäuren Lys95 und Arg96 die Interaktion mit der kleinen Furche der DNA vermitteln. Die Ergebnisse dieser Arbeit postulieren ein Modell zur Signalverarbeitung in CadC, in welchem die Signalwahrnehmung im Periplasma zu konformationellen Veränderungen des unstrukturierten CadC-Linkers führt und somit die räumliche Positionierung der DNA-Bindedomänen im CadC-Dimer ermöglicht wird.

Caribbean Radio Show Crs Radio
Meditations Ansel Cridland tells ALL,Reggae and Bob Marley

Caribbean Radio Show Crs Radio

Play Episode Listen Later Jun 28, 2013 239:00


The Meditations are a reggae vocal harmony group from Jamaica formed in late 1974. They have released several studio albums and are still performing in the 2000s and today.The Meditations were formed in late 1974, when Danny Clarke left The Righteous Flames, recruiting Ansel Cridland (previously of The Linkers) and Winston Watson. After releasing singles credited to the individual members, they began recording as The Meditations in late 1976, shortly after which they released their biggest hit, "Woman Is Like a Shadow", which sold over 45,000 copies in its first month of release. They recorded in the mid-1970s for producers such as Dobby Dobson, Joseph Hoo Kim, and Lee "Scratch" Perry, their righteously Rastafarian style gaining comparisons with The Mighty Diamonds.Their first album, Message From The Meditations, was released in 1977.The Meditations sang backing vocals on a number of Bob Marley songs, including "Blackman Redemption," "Punky Reggae Party," and "Rastaman Live Up", as well as providing backing for Gregory Isaacs, Jimmy Cliff and The Congos (on their Heart of the Congos album).They appeared at the One Love Peace Concert in April 1978, officially a commemoration of the 12th anniversary of Haile Selassie's state visit to Jamaica, but more famous for the handshake between Michael Manley and Edward Seaga when they joined Bob Marley on stage.The Meditations 1983 album, No More Friend was produced by Linval Thompson and featured The Roots Radics, and saw them adapting to the prevailing dancehall sound.Cridland left the group, leaving Clarke and Watson to record 1988's For The Good of Man without him, but they were reunited for 1993's . http://en.wikipedia.org/wiki/The_Meditations caribbeanradioshow@gmail.com  661-467-2407 www.crsradio.com www.caribbeanradioshow.com

Ca va trancher
Ca va trancher 35

Ca va trancher

Play Episode Listen Later Oct 5, 2011 81:17


35ème numéro de Ça va trancher en compagnie d'Alex du podcast Les Dessous du Troll. On y parle encore de plein de choses drôles, comme Indochine, Les Simpsons, ou encore Camping Paradis (si si !), et on était pour la première fois en live/chat/vidéo sur https://www.badgeek.fr/live/, ce qui a entrainé pas mal de fous rires... Rejoignez-nous la semaine prochaine ! Voici les liens du web : http://www.youtube.com/watch?v=vIglHv0Vg1g&NR=1 http://youtu.be/XloiggwBL5c http://youtu.be/dKwzZZKIbUs http://www.asmeudon.asso.fr/1024_768/index.htm http://danstonchat.com/12605.html http://www.koreus.com/video/fox-retro.html Et voici pour le nanar, Blood Freak : http://www.nanarland.com/Chroniques/Main.php?id_film=bloodfreak http://www.youtube.com/user/BadGeekFR?feature=mhee#p/c/4954BDAEE504D5A9

Around the World with HSFA
Adventure in Afghanistan: Bamiyan Statues (1973)

Around the World with HSFA

Play Episode Listen Later Sep 27, 2011


View the splendor of these 6th century Buddhist statues, a UNESCO World Heritage Site. A special treat is the Linkers winding their way to the top of the statues through caves carved in the hillsides and painted by Buddhist monks. The film clip is from an episode of a popular southern California television travel-adventure series created by the Linker family, Hal and Halla and their son David. Their popular broadcasts, running from the 1950s-1970s, were known under the series titles of Three Passports to Adventure, Wonders of the World and The Wild, the Weird and the Wonderful. (This clip is drawn from HSFA film, accession number 2002.16.57. More information is available in SIRIS, the Smithsonian’s online catalog—see sidebar for URL.) COLOR and SOUND.

Fakultät für Chemie und Pharmazie - Digitale Hochschulschriften der LMU - Teil 03/06
Synthese und Etablierung eines photolabilen Linkers zur Metabolitanreicherung und Synthese und Einsatz von Naturstoffderivaten als molekulare Sonden für Activity-Based Protein Profiling

Fakultät für Chemie und Pharmazie - Digitale Hochschulschriften der LMU - Teil 03/06

Play Episode Listen Later Dec 10, 2010


Fri, 10 Dec 2010 12:00:00 +0100 https://edoc.ub.uni-muenchen.de/12416/ https://edoc.ub.uni-muenchen.de/12416/1/Frohnapfel_Ronald.pdf Frohnapfel, Ronald

Nintendo-difference podcast
Les linkers, NSMBW, Zelda et notre s

Nintendo-difference podcast

Play Episode Listen Later Dec 20, 2009 33:49


Comme promis, le Podcast Nintendo-Difference fait son retour cette semaine avec une