Podcasts about gm1

  • 41PODCASTS
  • 77EPISODES
  • 40mAVG DURATION
  • ?INFREQUENT EPISODES
  • May 22, 2025LATEST

POPULARITY

20172018201920202021202220232024


Best podcasts about gm1

Latest podcast episodes about gm1

The Border Patrol w/Steven St. John and Nate Bukaty
5-22-25 Thursday Hour 2 of New Day: ft. Jeff Chadiha

The Border Patrol w/Steven St. John and Nate Bukaty

Play Episode Listen Later May 22, 2025 35:34


In the 2nd hour of the show Steven is joined by Jeff Chadiha from NFL Network. Jeff talks to the guys about the crazy comeback the Pacers had over the Knicks in Gm1 of the Eastern Conference Finals. Then the guys discuss the passing of Colts owner Jim Irsay and the owners trying to ban the Tush Push. Also they talk about the NFL allowing players to participate in Flag Football in the Olympics. See omnystudio.com/listener for privacy information.

フミ子の雑談
マダムたちとのおしゃべり from Radiotalk

フミ子の雑談

Play Episode Listen Later Dec 11, 2024 12:00


➤フミ子イベント予定情報・申込 https://lit.link/fumikoviolin 【イベントスケジュール】 ➤12/13譜読(渋谷)ビバルディGm1楽章 ➤12/14 XmasLive(神楽坂) ➤12/14ニコ生配信 ➤12/15譜読(上野)ビバルディAm1楽章 ➤12/15弾こう会(上野) ➤12/15譜読(上野)クリスマス曲 ➤12/15深堀(上野)ポジション ➤12/20ニコ生 ➤12/21譜読(天王寺)戦メリ/タイタニック ➤12/21譜読(天王寺)アベマリア祭 ➤12/22 JUGENと弾こう会(天満橋) ➤12/22 JUGENレコ発(天満橋) ➤12/24 Violin2本LIVE(淀屋橋) ➤12/27 ニコ生 ➤12/28譜読(天王寺)ドッペル1楽章 ➤12/30 JUGENアンラインサロン配信 ​─────── #FUMIKO202412

フミ子の雑談
フミ子と梅田さんの関係 from Radiotalk

フミ子の雑談

Play Episode Listen Later Dec 9, 2024 12:01


➤フミ子イベント予定情報・申込 https://lit.link/fumikoviolin 【イベントスケジュール】 ➤12/13譜読(渋谷)ビバルディGm1楽章 ➤12/14 XmasLive(神楽坂) ➤12/14ニコ生配信 ➤12/15譜読(上野)ビバルディAm1楽章 ➤12/15弾こう会(上野) ➤12/15譜読(上野)クリスマス曲 ➤12/15深堀(上野)ポジション ➤12/20ニコ生 ➤12/21譜読(天王寺)戦メリ/タイタニック ➤12/21譜読(天王寺)アベマリア祭 ➤12/22 JUGENと弾こう会(天満橋) ➤12/22 JUGENレコ発(天満橋) ➤12/24 Violin2本LIVE(淀屋橋) ➤12/27 ニコ生 ➤12/28譜読(天王寺)ドッペル1楽章 ➤12/30 JUGENアンラインサロン配信 ​─────── #FUMIKO202412

フミ子の雑談
引越しのおっちゃんズ&親戚のおっちゃん from Radiotalk

フミ子の雑談

Play Episode Listen Later Dec 6, 2024 11:56


➤フミ子イベント予定情報・申込 https://lit.link/fumikoviolin 【イベントスケジュール】 ➤12/7 TOPSY LIVE(ケント) ➤12/13譜読(渋谷)ビバルディGm1楽章 ➤12/14 XmasLive(神楽坂) ➤12/14ニコ生配信 ➤12/15譜読(上野)ビバルディAm1楽章 ➤12/15弾こう会(上野) ➤12/15譜読(上野)クリスマス曲 ➤12/15深堀(上野)ポジション ➤12/20ニコ生 ➤12/21譜読(天王寺)戦メリ/タイタニック ➤12/21譜読(天王寺)アベマリア祭 ➤12/22 JUGENと弾こう会(天満橋) ➤12/22 JUGENレコ発(天満橋) ➤12/24 Violin2本LIVE(淀屋橋) ➤12/27 ニコ生 ➤12/28譜読(天王寺)ドッペル1楽章 ➤12/30 JUGENアンラインサロン配信 ​─────── #FUMIKO202412

フミ子の雑談
今回の引越しは荷物が多い from Radiotalk

フミ子の雑談

Play Episode Listen Later Dec 4, 2024 11:55


➤フミ子イベント予定情報・申込 https://lit.link/fumikoviolin 【イベントスケジュール】 ➤12/6ニコ生 ➤12/7 TOPSY LIVE(ケント) ➤12/13譜読(渋谷)ビバルディGm1楽章 ➤12/14 XmasLive(神楽坂) ➤12/14ニコ生配信 ➤12/15譜読(上野)ビバルディAm1楽章 ➤12/15弾こう会(上野) ➤12/15譜読(上野)クリスマス曲 ➤12/15深堀(上野)ポジション ➤12/20ニコ生 ➤12/21譜読(天王寺)戦メリ/タイタニック ➤12/21譜読(天王寺)アベマリア祭 ➤12/22 JUGENと弾こう会(天満橋) ➤12/22 JUGENレコ発(天満橋) ➤12/24 Violin2本LIVE(淀屋橋) ➤12/27 ニコ生 ➤12/28譜読(天王寺)ドッペル1楽章 ➤12/30 JUGENアンラインサロン配信 ​─────── #FUMIKO202412

フミ子の雑談
各種ご案内と新居でまずやりたいこと from Radiotalk

フミ子の雑談

Play Episode Listen Later Dec 4, 2024 11:52


➤フミ子イベント予定情報・申込 https://lit.link/fumikoviolin 【イベントスケジュール】 ➤12/6ニコ生 ➤12/7 TOPSY LIVE(ケント) ➤12/13譜読(渋谷)ビバルディGm1楽章 ➤12/14 XmasLive(神楽坂) ➤12/14ニコ生配信 ➤12/15譜読(上野)ビバルディAm1楽章 ➤12/15弾こう会(上野) ➤12/15譜読(上野)クリスマス曲 ➤12/15深堀(上野)ポジション ➤12/20ニコ生 ➤12/21譜読(天王寺)戦メリ/タイタニック ➤12/21譜読(天王寺)アベマリア祭 ➤12/22 JUGENと弾こう会(天満橋) ➤12/22 JUGENレコ発(天満橋) ➤12/24 Violin2本LIVE(淀屋橋) ➤12/27 ニコ生 ➤12/28譜読(天王寺)ドッペル1楽章 ➤12/30 JUGENアンラインサロン配信 ​─────── #FUMIKO202412

フミ子の雑談
12月以降の準備をしながら from Radiotalk

フミ子の雑談

Play Episode Listen Later Dec 2, 2024 11:58


➤フミ子イベント予定情報・申込 https://lit.link/fumikoviolin 【イベントスケジュール】 ➤12/3フミ子WinterLive(天満橋)配信アリ ➤12/6ニコ生 ➤12/7 TOPSY LIVE(ケント) ➤12/13譜読(渋谷)ビバルディGm1楽章 ➤12/14 XmasLive(神楽坂) ➤12/14ニコ生配信 ➤12/15譜読(上野)ビバルディAm1楽章 ➤12/15弾こう会(上野) ➤12/15譜読(上野)クリスマス曲 ➤12/15深堀(上野)ポジション ➤12/20ニコ生 ➤12/21譜読(天王寺)戦メリ/タイタニック ➤12/21譜読(天王寺)アベマリア祭 ➤12/22 JUGENと弾こう会(天満橋) ➤12/22 JUGENレコ発(天満橋) ➤12/24 Violin2本LIVE(淀屋橋) ➤12/27 ニコ生 ➤12/28譜読(天王寺)ドッペル1楽章 ➤12/30 JUGENアンラインサロン配信 ​─────── #FUMIKO202412

フミ子の雑談
音程にこだわりすぎると上達が遅れる問題 from Radiotalk

フミ子の雑談

Play Episode Listen Later Nov 28, 2024 12:00


➤フミ子イベント予定情報・申込 https://lit.link/fumikoviolin 【イベントスケジュール】 ➤11/29ニコ生 ​─────── ➤12/3フミ子WinterLive(天満橋)配信アリ ➤12/6ニコ生 ➤12/7 TOPSY LIVE(ケント) ➤12/13譜読(渋谷)ビバルディGm1楽章 ➤12/14 XmasLive(神楽坂) ➤12/14ニコ生配信 ➤12/15譜読(上野)ビバルディAm1楽章 ➤12/15弾こう会(上野) ➤12/15譜読(上野)クリスマス曲 ➤12/15深堀(上野)ポジション ➤12/20ニコ生 ➤12/21譜読(天王寺)戦メリ/タイタニック ➤12/21譜読(天王寺)アベマリア祭 ➤12/22 JUGENと弾こう会(天満橋) ➤12/22 JUGENレコ発(天満橋) ➤12/24 Violin2本LIVE(淀屋橋) ➤12/27 ニコ生 ➤12/28譜読(天王寺)ドッペル1楽章 ➤12/30 JUGENアンラインサロン配信 ​─────── #FUMIKO202411

フミ子の雑談
新アルバムのミキシング from Radiotalk

フミ子の雑談

Play Episode Listen Later Nov 26, 2024 11:35


➤フミ子イベント予定情報・申込 https://lit.link/fumikoviolin 【イベントスケジュール】 ➤11/28JUGENオンラインサロン配信 ➤11/29ニコ生 ​─────── ➤12/3フミ子WinterLive(天満橋)配信アリ ➤12/6ニコ生 ➤12/7 TOPSY LIVE(ケント) ➤12/13譜読(渋谷)ビバルディGm1楽章 ➤12/14 XmasLive(神楽坂) ➤12/14ニコ生配信 ➤12/15譜読(上野)ビバルディAm1楽章 ➤12/15弾こう会(上野) ➤12/15譜読(上野)クリスマス曲 ➤12/15深堀(上野)ポジション ➤12/20ニコ生 ➤12/21譜読(天王寺)戦メリ/タイタニック ➤12/21譜読(天王寺)アベマリア祭 ➤12/22 JUGENと弾こう会(天満橋) ➤12/22 JUGENレコ発(天満橋) ➤12/24 Violin2本LIVE(淀屋橋) ➤12/27 ニコ生 ➤12/28譜読(天王寺)ドッペル1楽章 ➤12/30 JUGENアンラインサロン配信 ​─────── #FUMIKO202411

フミ子の雑談
ロングコートをなびかせてキャリーを忘れる from Radiotalk

フミ子の雑談

Play Episode Listen Later Nov 24, 2024 11:57


➤フミ子イベント予定情報・申込 https://lit.link/fumikoviolin 【イベントスケジュール】 ➤11/28JUGENオンラインサロン配信 ➤11/29ニコ生 ​─────── ➤12/3フミ子WinterLive(天満橋)配信アリ ➤12/6ニコ生 ➤12/7 TOPSY LIVE(ケント) ➤12/13譜読(渋谷)ビバルディGm1楽章 ➤12/14 XmasLive(神楽坂) ➤12/14ニコ生配信 ➤12/15譜読(上野)ビバルディAm1楽章 ➤12/15弾こう会(上野) ➤12/15譜読(上野)クリスマス曲 ➤12/15深堀(上野)ポジション ➤12/20ニコ生 ➤12/21譜読(天王寺)戦メリ/タイタニック ➤12/21譜読(天王寺)アベマリア祭 ➤12/22 JUGENと弾こう会(天満橋) ➤12/22 JUGENレコ発(天満橋) ➤12/24 Violin2本LIVE(淀屋橋) ➤12/27 ニコ生 ➤12/28譜読(天王寺)ドッペル1楽章 ➤12/30 JUGENアンラインサロン配信 ​─────── #FUMIKO202411

フミ子の雑談
新居の掃除 from Radiotalk

フミ子の雑談

Play Episode Listen Later Nov 20, 2024 11:55


➤フミ子イベント予定情報・申込 https://lit.link/fumikoviolin 【イベントスケジュール】 ➤11/22ニコ生 ➤11/23譜読(天王寺)みんなでMUSIC SUNDAY練 ➤11/23 MIKI弾こう会 ➤11/24みんなでMUSIC SUNDAY(天王寺) ➤11/28JUGENオンラインサロン配信 ➤11/29ニコ生 ​─────── ➤12/3フミ子WinterLive(天満橋)配信アリ ➤12/6ニコ生 ➤12/7 TOPSY LIVE(ケント) ➤12/13譜読(渋谷)ビバルディGm1楽章 ➤12/14 XmasLive(神楽坂) ➤12/14ニコ生配信 ➤12/15譜読(上野)ビバルディAm1楽章 ➤12/15弾こう会(上野) ➤12/15譜読(上野)クリスマス曲 ➤12/15深堀(上野)ポジション ➤12/20ニコ生 ➤12/21譜読(天王寺)戦メリ/タイタニック ➤12/21譜読(天王寺)アベマリア祭 ➤12/22 JUGENと弾こう会(天満橋) ➤12/22 JUGENレコ発(天満橋) ➤12/24 Violin2本LIVE(淀屋橋) ➤12/27 ニコ生 ➤12/28譜読(天王寺)ドッペル1楽章 ➤12/30 JUGENアンラインサロン配信 ​─────── #FUMIKO202411

フミ子の雑談
忙しい1週間でした from Radiotalk

フミ子の雑談

Play Episode Listen Later Nov 17, 2024 11:58


➤新作バイオリン弾き比べ2024 https://youtu.be/Aq8BB-ag41s ​─────── Radiotalk ➤フミ子イベント予定情報・申込 https://lit.link/fumikoviolin 【イベントスケジュール】 ➤11/22ニコ生 ➤11/23譜読(天王寺)みんなでMUSIC SUNDAY練 ➤11/23 MIKI弾こう会 ➤11/24みんなでMUSIC SUNDAY(天王寺) ➤11/28JUGENオンラインサロン配信 ➤11/29ニコ生 ​─────── ➤12/3フミ子WinterLive(天満橋)配信アリ ➤12/6ニコ生 ➤12/7 TOPSY LIVE(ケント) ➤12/13譜読(渋谷)ビバルディGm1楽章 ➤12/14 XmasLive(神楽坂) ➤12/14ニコ生配信 ➤12/15譜読(上野)ビバルディAm1楽章 ➤12/15弾こう会(上野) ➤12/15譜読(上野)クリスマス曲 ➤12/15深堀(上野)ポジション ➤12/20ニコ生 ➤12/21譜読(天王寺)戦メリ/タイタニック ➤12/21譜読(天王寺)アベマリア祭 ➤12/22 JUGENと弾こう会(天満橋) ➤12/22 JUGENレコ発(天満橋) ➤12/24 Violin2本LIVE(淀屋橋) ➤12/27 ニコ生 ➤12/28譜読(天王寺)ドッペル1楽章 ➤12/30 JUGENアンラインサロン配信 ​─────── #FUMIKO202411

フミ子の雑談
14曲アレンジ終了 from Radiotalk

フミ子の雑談

Play Episode Listen Later Nov 15, 2024 12:00


➤フミ子イベント予定情報・申込 https://lit.link/fumikoviolin 【イベントスケジュール】 ➤11/16アンサンブル譜よみ会(布引ハーブ園) ➤11/16弾こう会(布引ハーブ園) ➤11/17譜読(天王寺)ドッペル1楽章 ➤11/22ニコ生 ➤11/23譜読(天王寺)みんなでMUSIC SUNDAY練 ➤11/23 MIKI弾こう会 ➤11/24みんなでMUSIC SUNDAY(天王寺) ➤11/28JUGENオンラインサロン配信 ➤11/29ニコ生 ​─────── ➤12/3フミ子WinterLive(天満橋)配信アリ ➤12/6ニコ生 ➤12/7 TOPSY LIVE(ケント) ➤12/13譜読(渋谷)ビバルディGm1楽章 ➤12/14 XmasLive(神楽坂) ➤12/14ニコ生配信 ➤12/15譜読(上野)ビバルディAm1楽章 ➤12/15弾こう会(上野) ➤12/15譜読(上野)クリスマス曲 ➤12/15深堀(上野)ポジション ➤12/20ニコ生 ➤12/21譜読(天王寺)戦メリ/タイタニック ➤12/21譜読(天王寺)アベマリア祭 ➤12/22 JUGENと弾こう会(天満橋) ➤12/22 JUGENレコ発(天満橋) ➤12/24 Violin2本LIVE(淀屋橋) ➤12/27 ニコ生 ➤12/28譜読(天王寺)ドッペル1楽章 ➤12/30 JUGENアンラインサロン配信 ​─────── #FUMIKO202411

フミ子の雑談
大阪弁。 from Radiotalk

フミ子の雑談

Play Episode Listen Later Nov 13, 2024 11:53


➤フミ子イベント予定情報・申込 https://lit.link/fumikoviolin 【イベントスケジュール】 ➤11/16アンサンブル譜よみ会(布引ハーブ園) ➤11/16弾こう会(布引ハーブ園) ➤11/17譜読(天王寺)ドッペル1楽章 ➤11/22ニコ生 ➤11/23譜読(天王寺)みんなでMUSIC SUNDAY練 ➤11/23 MIKI弾こう会 ➤11/24みんなでMUSIC SUNDAY(天王寺) ➤11/28JUGENオンラインサロン配信 ➤11/29ニコ生配信 ​─────── ➤12/3フミ子WinterLive(天満橋)配信アリ ➤12/6ニコ生 ➤12/7 TOPSY LIVE(ケント) ➤12/13譜読(渋谷)ビバルディGm1楽章 ➤12/14 XmasLive(神楽坂) ➤12/14ニコ生配信 ➤12/15譜読(上野)ビバルディAm1楽章 ➤12/15弾こう会(上野) ➤12/15譜読(上野)クリスマス曲 ➤12/15深堀(上野)ポジション ➤12/20ニコ生 ➤12/21譜読(天王寺)戦メリ/タイタニック ➤12/21譜読(天王寺)アベマリア祭 ➤12/22 JUGENと弾こう会(天満橋) ➤12/22 JUGENレコ発(天満橋) ➤12/27 JUGENアンラインサロン配信 ➤12/28譜読(天王寺)ドッペル1楽章 ​─────── #FUMIKO202411

フミ子の雑談
布引ハーブ園/お坊さんの船/大人向け教材 from Radiotalk

フミ子の雑談

Play Episode Listen Later Nov 11, 2024 11:59


➤フミ子イベント予定情報・申込 https://lit.link/fumikoviolin 【イベントスケジュール】 ➤11/16アンサンブル譜よみ会(布引ハーブ園) ➤11/16弾こう会(布引ハーブ園) ➤11/17譜読(天王寺)ドッペル1楽章 ➤11/22ニコ生 ➤11/23譜読(天王寺)みんなでMUSIC SUNDAY練 ➤11/23 MIKI弾こう会 ➤11/24みんなでMUSIC SUNDAY(天王寺) ➤11/28JUGENオンラインサロン配信 ➤11/29ニコ生配信 ​─────── ➤12/3フミ子WinterLive(天満橋)配信アリ ➤12/6ニコ生 ➤12/7 TOPSY LIVE(ケント) ➤12/13譜読(渋谷)ビバルディGm1楽章 ➤12/14 XmasLive(神楽坂) ➤12/14ニコ生配信 ➤12/15譜読(上野)ビバルディAm1楽章 ➤12/15弾こう会(上野) ➤12/15譜読(上野)クリスマス曲 ➤12/15深堀(上野)ポジション ➤12/20ニコ生 ➤12/21譜読(天王寺)戦メリ/タイタニック ➤12/21譜読(天王寺)アベマリア祭 ➤12/22 JUGENと弾こう会(天満橋) ➤12/22 JUGENレコ発(天満橋) ➤12/27 JUGENアンラインサロン配信 ➤12/28譜読(天王寺)ドッペル1楽章 ​─────── #FUMIKO202411

フミ子の雑談
AIと話して泣いた話 from Radiotalk

フミ子の雑談

Play Episode Listen Later Nov 8, 2024 11:38


➤フミ子イベント予定情報・申込 https://lit.link/fumikoviolin 【イベントスケジュール】 ➤11/10譜読(天王寺)カイザー⑦⑧ ➤11/16アンサンブル譜よみ会(布引ハーブ園) ➤11/16弾こう会(布引ハーブ園) ➤11/17譜読(天王寺)ドッペル1楽章 ➤11/22ニコ生 ➤11/23譜読(天王寺)みんなでMUSIC SUNDAY練 ➤11/23 MIKI弾こう会 ➤11/24みんなでMUSIC SUNDAY(天王寺) ➤11/28JUGENオンラインサロン配信 ➤11/29ニコ生配信 ​─────── ➤12/3フミ子WinterLive(天満橋)配信アリ ➤12/6ニコ生 ➤12/7 TOPSY LIVE(ケント) ➤12/13譜読(渋谷)ビバルディGm1楽章 ➤12/14 XmasLive(神楽坂) ➤12/14ニコ生配信 ➤12/15譜読(上野)ビバルディAm1楽章 ➤12/15弾こう会(上野) ➤12/15譜読(上野)クリスマス曲 ➤12/15深堀(上野)ポジション ➤12/20ニコ生 ➤12/21譜読(天王寺)戦メリ/タイタニック ➤12/21譜読(天王寺)アベマリア祭 ➤12/22 JUGENと弾こう会(天満橋) ➤12/22 JUGENレコ発(天満橋) ➤12/27 JUGENアンラインサロン配信 ➤12/28譜読(天王寺)ドッペル1楽章 ​─────── #FUMIKO202411

フミ子の雑談
新作レコーディングしてます from Radiotalk

フミ子の雑談

Play Episode Listen Later Nov 6, 2024 12:00


➤フミ子イベント予定情報・申込 https://lit.link/fumikoviolin 【イベントスケジュール】 ➤11/8ニコ生 ➤11/9島村アンサンブル(梅田) ➤11/10譜読(天王寺)カイザー⑦⑧ ➤11/16アンサンブル譜よみ会(布引ハーブ園) ➤11/16弾こう会(布引ハーブ園) ➤11/17譜読(天王寺)ドッペル1楽章 ➤11/22ニコ生 ➤11/23譜読(天王寺)みんなでMUSIC SUNDAY練 ➤11/23 MIKI弾こう会 ➤11/24みんなでMUSIC SUNDAY(天王寺) ➤11/28JUGENオンラインサロン配信 ➤11/29ニコ生配信 ​─────── ➤12/3フミ子WinterLive(天満橋)配信アリ ➤12/6ニコ生 ➤12/7 TOPSY LIVE(ケント) ➤12/13譜読(渋谷)ビバルディGm1楽章 ➤12/14 XmasLive(神楽坂) ➤12/14ニコ生配信 ➤12/15譜読(上野)ビバルディAm1楽章 ➤12/15弾こう会(上野) ➤12/15譜読(上野)クリスマス曲 ➤12/15深堀(上野)ポジション ➤12/20ニコ生 ➤12/21譜読(天王寺)戦メリ/タイタニック ➤12/21譜読(天王寺)アベマリア祭 ➤12/22 JUGENと弾こう会(天満橋) ➤12/22 JUGENレコ発(天満橋) ➤12/27 JUGENアンラインサロン配信 ➤12/28譜読(天王寺)ドッペル1楽章 ​─────── #FUMIKO202411

フミ子の雑談
創造と生活のバランス from Radiotalk

フミ子の雑談

Play Episode Listen Later Nov 4, 2024 12:01


・擬似共演者 ・後方彼氏面(後方彼女面) ➤フミ子イベント予定情報・申込 https://lit.link/fumikoviolin 【イベントスケジュール】 ➤11/9島村アンサンブル(梅田) ➤11/10譜読(天王寺)カイザー⑦⑧ ➤11/15ニコ生 ➤11/16アンサンブル譜よみ会(布引ハーブ園) ➤11/16弾こう会(布引ハーブ園) ➤11/17譜読(天王寺)ドッペル1楽章 ➤11/22ニコ生 ➤11/23譜読(天王寺)みんなでMUSIC SUNDAY練 ➤11/23 MIKI弾こう会 ➤11/24みんなでMUSIC SUNDAY(天王寺) ➤11/28JUGENオンラインサロン配信 ➤11/29ニコ生配信 ​─────── ➤12/3フミ子WinterLive(天満橋)配信アリ ➤12/6ニコ生 ➤12/7 TOPSY LIVE(ケント) ➤12/13譜読(渋谷)ビバルディGm1楽章 ➤12/14 XmasLive(神楽坂) ➤12/14ニコ生配信 ➤12/15譜読(上野)ビバルディAm1楽章 ➤12/15弾こう会(上野) ➤12/15譜読(上野)クリスマス曲 ➤12/15深堀(上野)ポジション ➤12/20ニコ生 ➤12/21譜読(天王寺)戦メリ/タイタニック ➤12/21譜読(天王寺)アベマリア祭 ➤12/22 JUGENと弾こう会(天満橋) ➤12/22 JUGENレコ発(天満橋) ➤12/27 JUGENアンラインサロン配信 ➤12/28譜読(天王寺)ドッペル1楽章 ​─────── #FUMIKO202411

Noll till 100
38. På liv och död: vi går igenom allt som hänt sen sist

Noll till 100

Play Episode Listen Later Oct 31, 2024 60:12


I veckans avsnitt av Noll till 100 är Fedja äntligen tillbaka i studion. Han berättar för första gången om hans hälsotillstånd som fick honom att ta en paus från podden och hur han tränade ChatGPT att hjälpa honom. Jacob går även in på sin nya byrå och bryter ner ett case med både lärdomar och misstag. 1.00 min - Vad har hänt sen sist? Fedjas hälsotillstånd.27.30 min - Status just nu - hur mår Fedja idag? 37.00 min - Jacob har startat en byrå. 41.30 min - Crashcourse: Vad är GM1?45.52 min - Så kommer Jacob jobba framåt. 47.36 min - Inverterad Black Week. 50.40 min - Ett klassiskt problem e-handlare stöter på. 53.00 min - Vad händer när bolag växer för fort?Här hittar du Fedja & Jacob:https://se.linkedin.com/in/porobic https://www.linkedin.com/in/jacobwibomwesterberg/ Hosted on Acast. See acast.com/privacy for more information.

フミ子の雑談
今回のレコーディングは疲れない from Radiotalk

フミ子の雑談

Play Episode Listen Later Oct 30, 2024 12:00


10月ありがとうございました! ​─────── ➤フミ子イベント予定情報・申込 https://lit.link/fumikoviolin ​─────── 【イベントスケジュール】 ➤11/1ニコ生 ➤11/2譜読会(天王寺)ミニヨン/ボッケリーニ ➤11/2バイオリン2本LIVE(酒音) ➤11/4YouTube弾こう会 ➤11/4深堀講座(天王寺)重音 ➤11/8ニコ生 ➤11/9島村アンサンブル(梅田) ➤11/10譜読(天王寺)カイザー⑦⑧ ➤11/15ニコ生 ➤11/16アンサンブル譜よみ会(布引ハーブ園) ➤11/16弾こう会(布引ハーブ園) ➤11/17譜読(天王寺)ドッペル1楽章 ➤11/22ニコ生 ➤11/23譜読(天王寺)みんなでMUSIC SUNDAY練 ➤11/23 MIKI弾こう会 ➤11/24みんなでMUSIC SUNDAY(天王寺) ➤11/28JUGENオンラインサロン配信 ➤11/29ニコ生配信 ​─────── ➤12/3フミ子WinterLive(天満橋)配信アリ ➤12/6ニコ生 ➤12/7 TOPSY LIVE(ケント) ➤12/13譜読(渋谷)ビバルディGm1楽章 ➤12/14 XmasLive(神楽坂) ➤12/14ニコ生配信 ➤12/15譜読(上野)ビバルディAm1楽章 ➤12/15弾こう会(上野) ➤12/15譜読(上野)クリスマス曲 ➤12/15深堀(上野)ポジション ➤12/20ニコ生 ➤12/21譜読(天王寺)戦メリ/タイタニック ➤12/21譜読(天王寺)アベマリア祭 ➤12/22 JUGENと弾こう会(天満橋) ➤12/22 JUGENレコ発(天満橋) ➤12/27 JUGENアンラインサロン配信 ➤12/28譜読(天王寺)ドッペル1楽章 ​─────── #FUMIKO202410

フミ子の雑談
色々おしらせ from Radiotalk

フミ子の雑談

Play Episode Listen Later Oct 28, 2024 11:53


⚠️11/2(土)ナウシカの譜よみ会中止します ・11/2(土)鈴木2巻の譜よみ会はあります ・Radiotalk収録とライブ配信の頻度が下がります ​─────── ➤フミ子イベント予定情報・申込 https://lit.link/fumikoviolin 【イベントスケジュール】 ➤11/1ニコ生 ➤11/2譜読会(天王寺)ミニヨン/ボッケリーニ ➤11/2バイオリン2本LIVE(酒音) ➤11/4YouTube弾こう会 ➤11/4深堀講座(天王寺)重音 ➤11/8ニコ生 ➤11/9島村アンサンブル(梅田) ➤11/10譜読(天王寺)カイザー⑦⑧ ➤11/15ニコ生 ➤11/16アンサンブル譜よみ会(布引ハーブ園) ➤11/16弾こう会(布引ハーブ園) ➤11/17譜読(天王寺)ドッペル1楽章 ➤11/22ニコ生 ➤11/23譜読(天王寺)みんなでMUSIC SUNDAY練 ➤11/23 MIKI弾こう会 ➤11/24みんなでMUSIC SUNDAY(天王寺) ➤11/28JUGENオンラインサロン配信 ➤11/29ニコ生配信 ​─────── ➤12/3フミ子WinterLive(天満橋)配信アリ ➤12/6ニコ生 ➤12/7 TOPSY LIVE(ケント) ➤12/13譜読(渋谷)ビバルディGm1楽章 ➤12/14 XmasLive(神楽坂) ➤12/14ニコ生配信 ➤12/15譜読(上野)ビバルディAm1楽章 ➤12/15弾こう会(上野) ➤12/15譜読(上野)クリスマス曲 ➤12/15深堀(上野)ポジション ➤12/20ニコ生 ➤12/21譜読(天王寺)戦メリ/タイタニック ➤12/21譜読(天王寺)アベマリア祭 ➤12/22 JUGENと弾こう会(天満橋) ➤12/22 JUGENレコ発(天満橋) ​─────── #FUMIKO202410

フミ子の雑談
人間のレベル差 from Radiotalk

フミ子の雑談

Play Episode Listen Later Oct 26, 2024 11:59


➤フミ子イベント予定情報・申込 https://lit.link/fumikoviolin 【イベントスケジュール】 ➤11/1ニコ生 ➤11/2譜読(天王寺)風の谷のナウシカ/オープニング ➤11/2譜読会(天王寺)ミニヨン/ボッケリーニ ➤11/2バイオリン2本LIVE(酒音) ➤11/4YouTube弾こう会 ➤11/4深堀講座(天王寺)重音 ➤11/8ニコ生 ➤11/9島村アンサンブル(梅田) ➤11/10譜読(天王寺)輝く未来/自由への扉 ➤11/10譜読(天王寺)カイザー⑦⑧ ➤11/15ニコ生 ➤11/16アンサンブル譜よみ会(布引ハーブ園) ➤11/16弾こう会(布引ハーブ園) ➤11/17譜読(天王寺)ドッペル1楽章 ➤11/22ニコ生 ➤11/23譜読(天王寺)みんなでMUSIC SUNDAY練 ➤11/23 MIKI弾こう会 ➤11/24みんなでMUSIC SUNDAY(天王寺) ➤11/28JUGENオンラインサロン配信 ➤11/29ニコ生配信 ​─────── ➤12/1譜読(天王寺)冬1楽章 ➤12/1譜読(天王寺)カイザー⑨⑩ ➤12/3フミ子WinterLive(天満橋)配信アリ ➤12/6ニコ生 ➤12/7 TOPSY LIVE(ケント) ➤12/13譜読(渋谷)ビバルディGm1楽章 ➤12/14 XmasLive(神楽坂) ➤12/14ニコ生配信 ➤12/15譜読(上野)ビバルディAm1楽章 ➤12/15弾こう会(上野) ➤12/15譜読(上野)クリスマス曲 ➤12/15深堀(上野)ポジション ➤12/20ニコ生 ➤12/21譜読(天王寺)戦メリ/タイタニック ➤12/21譜読(天王寺)アベマリア祭 ➤12/22 JUGENと弾こう会(天満橋) ​─────── #FUMIKO202410

フミ子の雑談
おばあちゃん家にはコケシがある from Radiotalk

フミ子の雑談

Play Episode Listen Later Oct 25, 2024 11:49


➤フミ子イベント予定情報・申込 https://lit.link/fumikoviolin 【イベントスケジュール】 ​─────── ➤10/25ニコ生 ➤​10/27譜読(天王寺)ドッペル2&3 ​─────── ➤11/1ニコ生 ➤11/2譜読(天王寺)風の谷のナウシカ/オープニング ➤11/2譜読会(天王寺)ミニヨン/ボッケリーニ ➤11/2バイオリン2本LIVE(酒音) ➤11/4YouTube弾こう会 ➤11/4深堀講座(天王寺)重音 ➤11/8ニコ生 ➤11/9島村アンサンブル(梅田) ➤11/10譜読(天王寺)輝く未来/自由への扉 ➤11/10譜読(天王寺)カイザー⑦⑧ ➤11/15ニコ生 ➤11/16アンサンブル譜よみ会(布引ハーブ園) ➤11/16弾こう会(布引ハーブ園) ➤11/17譜読(天王寺)ドッペル1楽章 ➤11/22ニコ生 ➤11/23譜読(天王寺)みんなでMUSIC SUNDAY練 ➤11/23 MIKI弾こう会 ➤11/24みんなでMUSIC SUNDAY(天王寺) ➤11/28JUGENオンラインサロン配信 ➤11/29ニコ生配信 ​─────── ➤12/1譜読(天王寺)冬1楽章 ➤12/1譜読(天王寺)カイザー⑨⑩ ➤12/3フミ子WinterLive(天満橋)配信アリ ➤12/6ニコ生 ➤12/7 TOPSY LIVE(ケント) ➤12/13譜読(渋谷)ビバルディGm1楽章 ➤12/14 XmasLive(神楽坂) ➤12/14ニコ生配信 ➤12/15譜読(上野)ビバルディAm1楽章 ➤12/15弾こう会(上野) ➤12/15譜読(上野)クリスマス曲 ➤12/15深堀(上野)ポジション ➤12/20ニコ生 ➤12/21譜読(天王寺)戦メリ/タイタニック ​─────── #FUMIKO202410

フミ子の雑談
レッスンあれこれ from Radiotalk

フミ子の雑談

Play Episode Listen Later Oct 24, 2024 11:55


➤フミ子イベント予定情報・申込 https://lit.link/fumikoviolin 【イベントスケジュール】 ​─────── ➤10/25ニコ生 ➤​10/27譜読(天王寺)ドッペル2&3 ➤10/27 Radiotalk演奏配信 ​─────── ➤11/1ニコ生 ➤11/2譜読(天王寺)風の谷のナウシカ/オープニング ➤11/2譜読会(天王寺)ミニヨン/ボッケリーニ ➤11/2バイオリン2本LIVE(酒音) ➤11/4YouTube弾こう会 ➤11/4深堀講座(天王寺)重音 ➤11/8ニコ生 ➤11/9島村アンサンブル(梅田) ➤11/10譜読(天王寺)輝く未来/自由への扉 ➤11/10譜読(天王寺)カイザー⑦⑧ ➤11/15ニコ生 ➤11/16アンサンブル譜よみ会(布引ハーブ園) ➤11/16弾こう会(布引ハーブ園) ➤11/17譜読(天王寺)ドッペル1楽章 ➤11/22ニコ生 ➤11/23譜読(天王寺)みんなでMUSIC SUNDAY練 ➤11/23 MIKI弾こう会 ➤11/24みんなでMUSIC SUNDAY(天王寺) ➤11/28JUGENオンラインサロン配信 ➤11/29ニコ生配信 ​─────── ➤12/1譜読(天王寺)冬1楽章 ➤12/1譜読(天王寺)カイザー⑨⑩ ➤12/3フミ子WinterLive(天満橋)配信アリ ➤12/6ニコ生 ➤12/7 TOPSY LIVE(ケント) ➤12/13譜読(渋谷)ビバルディGm1楽章 ➤12/14 XmasLive(神楽坂) ➤12/14ニコ生配信 ➤12/15譜読(上野)ビバルディAm1楽章 ➤12/15弾こう会(上野) ➤12/15譜読(上野)クリスマス曲 ➤12/15深堀(上野)ポジション ➤12/20ニコ生 ​─────── #FUMIKO202410

フミ子の雑談
作業しながらバイオハザード(映画)シリーズを観ていました。 from Radiotalk

フミ子の雑談

Play Episode Listen Later Oct 23, 2024 11:58


➤フミ子イベント予定情報・申込 https://lit.link/fumikoviolin 【イベントスケジュール】 ​─────── ➤10/25ニコ生 ➤​10/27譜読(天王寺)ドッペル2&3 ➤10/27 Radiotalk演奏配信 ​─────── ➤11/1ニコ生 ➤11/2譜読(天王寺)風の谷のナウシカ/オープニング ➤11/2譜読会(天王寺)ミニヨン/ボッケリーニ ➤11/2バイオリン2本LIVE(酒音) ➤11/4YouTube弾こう会 ➤11/4深堀講座(天王寺)重音 ➤11/8ニコ生 ➤11/9島村アンサンブル(梅田) ➤11/10譜読(天王寺)輝く未来/自由への扉 ➤11/10譜読(天王寺)カイザー⑦⑧ ➤11/15ニコ生 ➤11/16アンサンブル譜よみ会(布引ハーブ園) ➤11/16弾こう会(布引ハーブ園) ➤11/17譜読(天王寺)ドッペル1楽章 ➤11/22ニコ生 ➤11/23譜読(天王寺)みんなでMUSIC SUNDAY練 ➤11/23 MIKI弾こう会 ➤11/24みんなでMUSIC SUNDAY(天王寺) ➤11/28JUGENオンラインサロン配信 ➤11/29ニコ生配信 ​─────── ➤12/1譜読(天王寺)冬1楽章 ➤12/1譜読(天王寺)カイザー⑨⑩ ➤12/3フミ子WinterLive(天満橋)配信アリ ➤12/6ニコ生 ➤12/7 TOPSY LIVE(ケント) ➤12/13譜読(渋谷)ビバルディGm1楽章 ➤12/14 XmasLive(神楽坂) ➤12/14ニコ生配信 ➤12/15譜読(上野)ビバルディAm1楽章 ➤12/15弾こう会(上野) ➤12/15譜読(上野)クリスマス曲 ➤12/15深堀(上野)ポジション ➤12/20ニコ生 ​─────── #FUMIKO202410

フミ子の雑談
指が動きにくい/ポジションの基準 from Radiotalk

フミ子の雑談

Play Episode Listen Later Oct 22, 2024 11:49


➤フミ子イベント予定情報・申込 https://lit.link/fumikoviolin 【イベントスケジュール】 ​─────── ➤​10/23 JUGENサロン配信 ➤10/25ニコ生 ➤10/26譜読(天王寺)クルエラ/これがハロウィン ➤10/26譜読(天王寺)糸/千本桜 ➤​10/27譜読(天王寺)ドッペル2&3 ➤10/27 Radiotalk演奏配信 ​─────── ➤11/1ニコ生 ➤11/2譜読(天王寺)風の谷のナウシカ/オープニング ➤11/2譜読会(天王寺)ミニヨン/ボッケリーニ ➤11/2バイオリン2本LIVE(酒音) ➤11/4YouTube弾こう会 ➤11/4深堀講座(天王寺)重音 ➤11/8ニコ生 ➤11/9島村アンサンブル(梅田) ➤11/10譜読(天王寺)輝く未来/自由への扉 ➤11/10譜読(天王寺)カイザー⑦⑧ ➤11/15ニコ生 ➤11/16アンサンブル譜よみ会(布引ハーブ園) ➤11/16弾こう会(布引ハーブ園) ➤11/17譜読(天王寺)ドッペル1楽章 ➤11/22ニコ生 ➤11/23譜読(天王寺)みんなでMUSIC SUNDAY練 ➤11/23 MIKI弾こう会 ➤11/24みんなでMUSIC SUNDAY(天王寺) ➤11/28JUGENオンラインサロン配信 ➤11/29ニコ生配信 ​─────── ➤12/1譜読(天王寺)冬1楽章 ➤12/1譜読(天王寺)カイザー⑨⑩ ➤12/3フミ子WinterLive(天満橋)配信アリ ➤12/6ニコ生 ➤12/7 TOPSY LIVE(ケント) ➤12/13譜読(渋谷)ビバルディGm1楽章 ➤12/14 XmasLive(神楽坂) ➤12/14ニコ生配信 ➤12/15譜読(上野)ビバルディAm1楽章 ​─────── #FUMIKO202410

フミ子の雑談
少数派から見るコミニュケーション from Radiotalk

フミ子の雑談

Play Episode Listen Later Oct 20, 2024 12:01


Radiotalk ➤フミ子イベント予定情報・申込 https://lit.link/fumikoviolin 【イベントスケジュール】 ​─────── ➤​10/23 JUGENサロン配信 ➤10/25ニコ生 ➤10/26譜読(天王寺)クルエラ/これがハロウィン ➤10/26譜読(天王寺)糸/千本桜 ➤​10/27譜読(天王寺)ドッペル2&3 ➤10/27 Radiotalk演奏配信 ​─────── ➤11/1ニコ生 ➤11/2譜読(天王寺)風の谷のナウシカ/オープニング ➤11/2譜読会(天王寺)ミニヨン/ボッケリーニ ➤11/2バイオリン2本LIVE(酒音) ➤11/4YouTube弾こう会 ➤11/4深堀講座(天王寺)重音 ➤11/8ニコ生 ➤11/9島村アンサンブル(梅田) ➤11/10譜読(天王寺)輝く未来/自由への扉 ➤11/10譜読(天王寺)カイザー⑦⑧ ➤11/15ニコ生 ➤11/16アンサンブル譜よみ会(布引ハーブ園) ➤11/16弾こう会(布引ハーブ園) ➤11/17譜読(天王寺)ドッペル1楽章 ➤11/22ニコ生 ➤11/23譜読(天王寺)みんなでMUSIC SUNDAY練 ➤11/23 MIKI弾こう会 ➤11/24みんなでMUSIC SUNDAY(天王寺) ➤11/28JUGENオンラインサロン配信 ➤11/29ニコ生配信 ​─────── ➤12/1譜読(天王寺)冬1楽章 ➤12/1譜読(天王寺)カイザー⑨⑩ ➤12/3フミ子WinterLive(天満橋)配信アリ ➤12/6ニコ生 ➤12/7 TOPSY LIVE(ケント) ➤12/13譜読(渋谷)ビバルディGm1楽章 ➤12/14 XmasLive(神楽坂) ➤12/14ニコ生配信 ➤12/15譜読(上野)ビバルディAm1楽章 ​─────── #FUMIKO202410

The Bernie Show
The Lede - Segment 1 6-12-24

The Bernie Show

Play Episode Listen Later Jun 12, 2024 31:49


Bernie gives his take on the pitching duel between Miles Mikolas and Paul Skenes from Gm1 of the Cardinals versus Pirates. Bernie also explains why he think Cardinals fans are some of the best in baseball and why they reignite his love for covering professional sports everyday. 

The Rich Eisen Show
Chris Mannix: Pacers played well and then they blew it

The Rich Eisen Show

Play Episode Listen Later May 22, 2024 54:23


Rich and Boston fan Chris Brockman break down the Celtics' escaping with an OT win after the Pacers looked poised to win Game 1 of their Eastern Conference Finals series. Sports Illustrated's Chris Mannix and Rich discuss the Pacers' late-game collapse in GM1 vs the Celtics, why Boston would prefer to face the Mavericks over the Timberwolves if they reach the NBA Finals, why the Mike Tyson/Jake Paul upcoming fight will be “goofy,” his reaction to Oleksandr Usyk's split decision win over Tyson Fury in their recent heavyweight title fight, and more.   Rich defends his honor when questioned about his recent near hole-in-one while golfing.  Please check out other RES productions: Overreaction Monday: http://apple.co/overreactionmonday  What the Football with Suzy Shuster and Amy Trask: http://apple.co/whatthefootball Learn more about your ad choices. Visit podcastchoices.com/adchoices

The Rich Eisen Show
Brian Anderson: there is a fever pitch for Jalen Brunson in NYC

The Rich Eisen Show

Play Episode Listen Later May 8, 2024 50:33


Rich and the guys recap the Celtics' GM1 rout of the Cavaliers and the Thunder doing likewise to Luka Doncic and the Mavericks, ponder what shape the Clippers would be in if they hadn't traded Shai Gilgeous-Alexander, and Rich offers up his daily dose of praise for Knicks guard Jalen Brunson. The NBA on TNT announcer Brian Anderson and Rich discuss how far Jalen Brunson can take the Knicks in the NBA Playoffs this season, the rise of Anthony Edwards and the Minnesota Timberwolves, his favorite moments working with legendary Milwaukee Brewers announcer Bob Uecker, and reveals what it was like being Al Michaels' runner when he was first starting out in the business. Rich and the guys react to the New York Rangers improving to 6-0 in the Stanley Cup Playoffs. Please check out other RES productions: Overreaction Monday: http://apple.co/overreactionmonday  What the Football with Suzy Shuster and Amy Trask: http://apple.co/whatthefootball Learn more about your ad choices. Visit podcastchoices.com/adchoices

Hochman and Crowder
Not time to panic despite a brutal Game 1 loss for the Cats

Hochman and Crowder

Play Episode Listen Later May 7, 2024 42:20


In hour one, what the hell happened to the Cats last night? Hoch and Crowder were definitely not expecting a blowout GM1 loss and Solana apologizes to Brooks Koepka because ultimately Koepka was right about Ekblad being a traffic cone. Creating a Mt. Rushmore of Al Pacino movies. Plus, Mike Cugno of CBS4 joins the show to recap the Panthers loss and to discuss Riley's comments regarding Jimmy Butler yesterday. 

The Short Shift Podcast
Ep.162 - Toaster Bath!

The Short Shift Podcast

Play Episode Listen Later Apr 12, 2024 82:07


Thomas bailed and the kids are running the show. Andrew and Ian discuss process over results; 9 game stretch against all playoff teams: 5-4-0... absolute stinker against Carolina notwithstanding. What are you taking from the totality of this run? Are we past the point of “let Swayman play out of it” and having Ullmark in pen GM1? Are you ready to declare the lineup for GM1? Plus: 7th player award, Torts torting, and the shitstorm that is the Arizona Coyotes. GET IN. Learn more about your ad choices. Visit megaphone.fm/adchoices

The Short Shift Podcast
Ep.162 - Toaster Bath!

The Short Shift Podcast

Play Episode Listen Later Apr 12, 2024 80:22


Thomas bailed and the kids are running the show. Andrew and Ian discuss process over results; 9 game stretch against all playoff teams: 5-4-0... absolute stinker against Carolina notwithstanding. What are you taking from the totality of this run? Are we past the point of “let Swayman play out of it” and having Ullmark in pen GM1? Are you ready to declare the lineup for GM1? Plus: 7th player award, Torts torting, and the shitstorm that is the Arizona Coyotes. GET IN.

Framtidens E-Handel
212. Aida Jammal: Investment director på eEquity - Så gör du en exit & säljer ditt e-handelsbolag

Framtidens E-Handel

Play Episode Listen Later Dec 1, 2023 76:19


eEquitys Aida Jammal gästar podden Framtidens E-Handel och pratar om hur man gör en exit som e-handlare.5 min - Om företagen som gått i konkurs, finns det en gemensam nämnare? 8 min - När är bästa tiden att sälja ditt bolag? Vilka olika typer av exits man kan göra, och deras för och nackdelar. Hur stor måste företaget vara?18 min - Hur ser försäljningsprocessen ut? Hur effektiviserar man GM1, GM2 och GM3, och hur maxar man likviditeten? 32 min - Vad ska man tänka på när man väljer köpare till sitt bolag? Vilken tid är bäst? Och borde man som grundare stanna kvar i bolaget efter exit? Vad kan eventuellt sänka ens värdering? 67 min - Tankar kring post-exit depression. Tidigare i avsnitt 186 & 200Här hittar du Aida:https://www.linkedin.com/in/aidajammal/ Följ Björn på LinkedIn:https://www.linkedin.com/in/bjornspenger/ Följ Framtidens E-handel på LinkedIn:https://www.linkedin.com/company/framtidens-e-handel/ Besök vår hemsida & Instagram:https://www.framtidensehandel.se/ https://www.instagram.com/framtidens.ehandel/ Sponsor:https://www.juni.co/ Poddklippare Michaela Dorch:https://www.linkedin.com/in/michaela-dorch-6a9a84113/ Tusen tack för att du lyssnar!Support till showen http://supporter.acast.com/framtidens-e-handel. Hosted on Acast. See acast.com/privacy for more information.

The Ecommerce Uptick
The 3 metrics That Make or Break an Ecommerce Brand

The Ecommerce Uptick

Play Episode Listen Later Sep 4, 2023 10:14


Wake up call for DTC Ecom brands! Even though you are trending on social media. The hottest VC is funding you... You can brag about a 6x+ ROAS And the best influencer creatives... You will still fail without - Knowing your Life time customer value - Your unit economics (Gross Margin 1, Gross Margin 2 etc) - Your Cashflow budget So why is LTV important? Well how do you know what you can pay to aquire a customer if you don't know what they will generate over their lifetime and each year? Media buying is a game with dimishing returns and 1 winner (The house aka, Meta, Google): You may get a 10x ROAS if you spend $1000 a month, but a 7x if you spend 10k. This is simplified of course, but the more people you reach, the colder the audience gets. Eventually you will end up paying more to outbid others... You have to understand your LTV in order to understand what your CPA goals should be. And that is just the tip of the iceberg.. because you still need more data... Most brands don't do accounting well. Heck if i say GM1, GM2, most ecom brands have no clue what I am talking about... You need to understand your gross margins... GM1 is total revenue minus cost of goods. ie how much it cost for you to produce those products The formula for calculating is GM1 = (Total Revenue - COGS) / Total Revenue Why is it important? GM1 provides a fundamental measure of the profitability of a company's core operations without factoring in other indirect costs like operating expenses, marketing, or administrative costs. Yet.. we need to get deeper.. we need to understand GM2 Gross Margin 2 (GM2): Here is where it gets interesting. Adding in shipping costs, CC fees, Fulfillment fees(software, packaging etc.) The formula for calculating it = (Total Revenue - (COGS + Variable Operating Costs)) / Total Revenue So lets say you sell for $100 your GM1 is 60% (40% is eaten up by cost of goods sold) Your GM2 is 40% (packaging, shipping cost etc.) Now you are left with $40 before accounting for marketing and your operational expenses. Now if your CPA is $45, you may have a problem.. You are not breaking even... but what if your LTV is $190 after 4 months? Perhaps this is not so bad after all.. This is why the economics are so important. What is the cost of getting that additional $90 over 4 months So few brands have these numbers... Last but not least... Cashflow.. equipped with all these numbers and sales targets for each month, you can actually calculate your cashflow. Obviously you need to factor in when you have to pay wages, ads, inventory. Without this, you are working in the blind and may go in the negative some months... Heck you could go bankrupt because of it.. The less you know about your numbers, the less aggressive you can be in scaling up your ecom brand... If you us to help you in this area, reach out to me on daniel@purplefire.io

PaperPlayer biorxiv neuroscience
Altered GM1 catabolism affects NMDAR-mediated Ca2+ signaling at ER-PM junctions and increases synaptic spineformation

PaperPlayer biorxiv neuroscience

Play Episode Listen Later Jul 11, 2023


Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2023.07.10.548446v1?rss=1 Authors: Weesner, J. A., Annunziata, I., van de Vlekkert, D., Robinson, C. G., Campos, Y., Mishra, A., Fremuth, L. E., Gomero, E., Hu, H., d'Azzo, A. Abstract: Endoplasmic reticulum-plasma membrane (ER-PM) junctions mediate Ca2+ flux across neuronal membranes. The properties of these membrane contact sites are defined by their lipid content, but little attention has been given to glycosphingolipids (GSLs). Here, we show that GM1-ganglioside, an abundant GSL in neuronal membranes, is integral to ER-PM junctions; it interacts with synaptic proteins/receptors and regulates Ca2+ signaling. In a model of the neurodegenerative lysosomal storage disease, GM1-gangliosidosis, pathogenic accumulation of GM1 at ER-PM junctions due to {beta} galactosidase deficiency drastically alters neuronal Ca2+ homeostasis. Mechanistically, we show that GM1 interacts with the phosphorylated NMDAR Ca2 channel, thereby increasing Ca2+ flux, activating ERK signaling, and increasing the number of synaptic spines without increasing synaptic connectivity. Thus, GM1 clustering at ER-PM junctions alters synaptic plasticity and exacerbates the generalized neuronal cell death characteristic of GM1-gangliosidosis. Copy rights belong to original authors. Visit the link for more info Podcast created by Paper Player, LLC

Play Me or Fade Me Sports Betting Picks Podcast
Sports Betting Picks (2 is my least favorite number, April MLB Recap: Won 7.93 units, 4 MLB Baseball First 5 Inning Team Totals for Monday)

Play Me or Fade Me Sports Betting Picks Podcast

Play Episode Listen Later May 1, 2023 8:14


DraftKings will typically post First 5 Team Totals by 11:45p EST (BetOnline is one of the first to post offshore)Today's Bets: Padres Over 2.5 (-135), Mets Under 1.5 GM1 (-130), Cubs Over 2.5 +110), Giants Over 1.5 (-130)Twitter:  Mets GM1 Under 1.5 (-150)Note: For those of you that receive the newsletter, I have a mid-day play on the Dodgers -0.5 over the Phillies in the F5 today at -125. MLB:First 5: 69-48 (58.9%)Twitter: 14-16 (46.7%)Starting Bankroll: $1,000Yesterday: Lost $64.62Current Bankroll: $1,158.67YTD Bets: 147Winning Days: 17Losing Days: 13Push Days: 0 |   |  | Odds | Risk | Result | P/LMLB | Marlins | Under 1.5 | -110 |  $20.00  | LOSS |  $(20.00)MLB | Cubs  | Over 2.5 | 115 |  $20.00  | LOSS |  $(20.00)MLB | Angels | Over 2.5 | 125 |  $20.00  | LOSS |  $(20.00)MLB | Royals | Under 1.5 | -130 |  $20.00  | WIN  |  $15.38 T | Cubs  | Over 2.5 | 100 |  $20.00  | LOSS |  $(20.00)Total |  |  |  |  |   |  $(64.62)YTD Podcast Performance BE - Active:NoneStarting Bankroll: $1,000Yesterday: N/ACurrent Bankroll: $1,179.33YTD Bets: 705Winning Days: 48Losing Days: 34Push Days: 6Podcast Accomplishments:NFL Football: Hit 60.2% in the 2022 NFL Regular SeasonLongest Winning Streak: 7 days (March 21, 2022-March 27, 2022)NFL Prop Bets: 62.8% in 2021NFL Football: 57.7% winning over 100 bets in 2021MLB Baseball Team Totals: 213-159, 57.2%, won 44.37 units Want to support the show for $5/month & get a daily picks newsletter? https://www.buzzsprout.com/1836761/supportBettorEdge, Peer to peer sports betting with NO JUICE! Click the link for a risk free $20, no deposit required. BettorEdge.com/playmePrizePicks Link:https://app.prizepicks.com/sign-up?invite_code=PLAYMEPROMO CODE: PLAYME for a 100% match up to $100Jay Buckley's Baseball Tours - 24 trips to choose from in 2023www.jaybuckley.com, referred by Jeremy Cleveland/Mr. Action JunkieSports Handicapping - What is a good winning percentage when betting on sports? http://professionalgambler.org/winning-percentagesContact Me: Twitter: @MrActionJunkie1 Email: mractionjunkie@gmail.comFollow me on BettorEdge: @mractionjunkieMy favorite podcasts: Sportsline Early Edge, Daily Juice2023 Accountability Tracker: 66/365 days (I love food, I can't stop eating but I will exercise in 2023)Inspired by: https://www.tampabay.com/health/medical-news/need-weight-loss-inspiration-walk-bayshore-with-a-man-who-is-pushing-3200-days-in-a-row-20190111/

Hockey Royalty Podcast
Ep109: The Comeback Kings

Hockey Royalty Podcast

Play Episode Listen Later Apr 19, 2023 58:01


Hockey Royalty talks about the Kings win in GM1 as well as setting the stage for GM2. Get in the chat, like and subscribe! Draft Kings Code: THPN #gokingsgo #LAKings #hockey #EdmontonOilers #THPN #draftkings #nhlhockey #nhlplayoffs If you or someone you know has a gambling problem, crisis counseling and referral services can be accessed by calling 1-800- GAMBLER (1-800-426-2537) (IL/IN/MI/NJ/PA/WV/WY), 1-800-NEXT STEP (AZ), 1-800-522-4700 (CO/NH), 888-789-7777/visit http://ccpg.org/chat (CT), 1-800-BETS OFF (IA), 1-877-770-STOP (7867) (LA), 877-8-HOPENY/text HOPENY (467369) (NY), visit OPGR.org (OR), call/text TN REDLINE 1-800-889-9789 (TN), or 1-888- 532-3500 (VA). 21+ (18+ WY). Physically present in AZ/CO/CT/IL/IN/IA/LA/MI/ /NJ/NY/ PA/TN/VA/WV/WY only. New customers only. Min. $5 deposit required. Eligibility restrictions apply. See http://draftkings.com/sportsbook for details. Learn more about your ad choices. Visit megaphone.fm/adchoices

Hockey Royalty Podcast
Ep109: The Comeback Kings

Hockey Royalty Podcast

Play Episode Listen Later Apr 19, 2023 56:01


Hockey Royalty talks about the Kings win in GM1 as well as setting the stage for GM2. Get in the chat, like and subscribe!Draft Kings Code: THPN#gokingsgo #LAKings #hockey #EdmontonOilers #THPN #draftkings #nhlhockey #nhlplayoffsIf you or someone you know has a gambling problem, crisis counseling and referral services can be accessed by calling 1-800- GAMBLER (1-800-426-2537) (IL/IN/MI/NJ/PA/WV/WY), 1-800-NEXT STEP (AZ), 1-800-522-4700 (CO/NH), 888-789-7777/visit http://ccpg.org/chat (CT), 1-800-BETS OFF (IA), 1-877-770-STOP (7867) (LA), 877-8-HOPENY/text HOPENY (467369) (NY), visit OPGR.org (OR), call/text TN REDLINE 1-800-889-9789 (TN), or 1-888- 532-3500 (VA).21+ (18+ WY). Physically present in AZ/CO/CT/IL/IN/IA/LA/MI/ /NJ/NY/ PA/TN/VA/WV/WY only. New customers only. Min. $5 deposit required. Eligibility restrictions apply. Seehttp://draftkings.com/sportsbook for details.

PaperPlayer biorxiv neuroscience
Naive Huntington's disease microglia mount a normal response to inflammatory stimuli but display impaired development of innate immune tolerance that can be counteracted by ganglioside GM1

PaperPlayer biorxiv neuroscience

Play Episode Listen Later Apr 6, 2023


Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2023.04.05.535712v1?rss=1 Authors: Steinberg, N., Galleguillos, D., Zaidi, A., Sipione, S. Abstract: Chronic activation and dysfunction of microglia have been implicated in the pathogenesis and progression of many neurodegenerative disorders, including Huntington Disease (HD). HD is a genetic condition caused by a mutation that affects the folding and function of huntingtin (HTT). Signs of microglia activation have been observed in HD patients even before the onset of symptoms. It is unclear, however, whether pro-inflammatory microglia activation in HD results from cell-autonomous expression of mutant HTT or is the response of microglia to a diseased brain environment, or both. In this study, we used primary microglia isolated from HD knock-in mice (Q140) and wild-type (Q7) mice to investigate their response to inflammatory conditions in vitro in the absence of confounding effects arising from brain pathology. We show that naive Q140 microglia do not undergo spontaneous pro-inflammatory activation and respond to inflammatory triggers, including stimulation of TLR4 and TLR2 and exposure to necrotic cells, with similar kinetics of pro-inflammatory gene expression as wild-type microglia. Upon termination of the inflammatory insult, the transcription of pro-inflammatory cytokines is tapered off in Q140 and wild-type microglia with similar kinetics. However, the ability of Q140 microglia to develop tolerance in response to repeated inflammatory stimulations is partially impaired, potentially contributing to the establishment of chronic neuroinflammation in HD. We further show that ganglioside GM1, a glycosphingolipid with anti-inflammatory effects in wild-type microglia, not only decreases the production of pro-inflammatory cytokines and nitric oxide in activated Q140 microglia, but also dramatically dampen microglia response to re-stimulation with LPS in an experimental model of tolerance. These effects are independent from the expression of interleukin 1 receptor associated kinase 3 (Irak-3), a strong modulator of LPS signaling involved in the development of innate immune tolerance and previously shown to be upregulated by immune cell treatment with gangliosides. Altogether, our data suggest that external triggers are required for HD microglia activation, but a cell-autonomous dysfunction that affects the ability of HD microglia to acquire tolerance might contribute to the establishment of neuroinflammation in HD. Administration of GM1 might be beneficial to attenuate chronic microglia activation and neuroinflammation. Copy rights belong to original authors. Visit the link for more info Podcast created by Paper Player, LLC

RARECast
Advancing a Gene Therapy for a Rare and Fatal CNS Disorder

RARECast

Play Episode Listen Later Mar 30, 2023 20:02


GM1 gangliosidosis is a rare and deadly lysosomal storage disorder that causes progressive damage to neurons in the brain, as well as the heart, liver, bones and other tissues throughout the body. There are currently no approved therapies to treat the condition. Passage Bio, which has a collaboration with the Gene Therapy Program at the University of Pennsylvania, is developing a gene therapy to the condition. We spoke to Samiah Al-Zaidy, vice president of clinical development for Passage, about GM1 gangliosidosis, the company's experimental therapy to treat the condition, and what's known about the therapy from work that's been done to date.

Permission To Speak Freely
[BONUS EPISODE] ”There's Always A Backstory”

Permission To Speak Freely

Play Episode Listen Later Mar 6, 2023 47:33


Damon is on vacation so Tisha and Damo are holding it down in this bonus episode of "Permission To Speak Freely." Recently a screenshot of a conversation between a GM1 who appears to be his LCPO surfaced on the internet. GM1 is asking for a "sick day" because he didn't get any sleep due to having to take care of his sick daughter while his wife was taking care of the newborn. Chief wasn't having any of it and told GM1 that he needs to be at work tomorrow. Tisha and Damo read through the text thread and have a "deep dive" conversation about the whole scenario. Stay tuned until the end, there may or may not be an appearance from a very special guest! Stay tuned until the end, there may or may not be an appearance from a very special guest! Remember to follow the ‘Permission to Speak Freely' podcast on Facebook, Instagram, Twitter, and subscribe on YouTube.   Link to all social media: https://linktr.ee/Ptsfpodcast   Intro Music Produced by Lim0

TerraSpaces
OmniFlix In the Spotlight: NFTs for GM1 and Mental Health Sandytoes2211 & Brainfever

TerraSpaces

Play Episode Listen Later Mar 4, 2023 89:01


Today on the Ether we have an episode of OmniFlix In the Spotlight on NFTs for mental health and GM1 with Sandytoes2211 and Brainfever, aka Daniel Lorent, hosted by Chev. Recorded on March 4th 2023. If you enjoy the music at the end of the episodes, you can find the albums streaming on Spotify, and the rest of your favorite streaming platforms. Check out Project Survival, Virus Diaries, and Plan B wherever you get your music. Thank you to everyone in the community who supports TerraSpaces.

Beyond Biotech - the podcast from Labiotech
Beyond Biotech podcast 35: Rare Disease Day

Beyond Biotech - the podcast from Labiotech

Play Episode Listen Later Feb 24, 2023 61:06


0:58 Labiotech.eu news2:37 Replay23:45 Azafaros39:39 GRO BiosciencesAs February 28 is Rare Disease Day, we have three guests on the podcast today all relevant to rare diseases. And they are Dan Mandell, CEO and co-founder of GRO Biosciences; Stefano Portolano, chief executive officer, Azafaros; and from Replay, Adrian Woolfson, executive chairman, president, and co-founder, and Lachlan MacKinnon, CEO and co-founder.Rare Disease DayTaking place on February 28 each year, the event raises awareness and looks to generate change for the 300 million people worldwide living with a rare disease, as well as their families and carers.This year, the day will be marked by more than 600 events in 106 countries.Replay and The University of Texas MD Anderson Cancer Center create SyenaReplay, a genome writing company reprogramming biology by writing and delivering big DNA, and The University of Texas MD Anderson Cancer Center, have launched Syena, a new oncology-focused product company pioneering T-cell receptor (TCR) natural killer (NK) cell therapies (TCR-NKs).Building on the intellectual property and technology from MD Anderson and Replay, Syena has the potential to create the next generation of cell therapy.AzafarosAzafaros is a clinical stage company founded in 2018 with a deep understanding of rare genetic disease mechanisms, and a compound library from Leiden University. Its aim is to build a pipeline of disease-modifying therapeutics to offer patients and their families new treatment options. The company's lead clinical-staged program is AZ-3102, a small molecule, orally available, brain penetrant azasugar, with the potential to treat GM1 gangliosidosis and GM2 gangliosidosis (Tay-Sachs and Sandhoff diseases) and Niemann-Pick disease type C (NP-C). This week, the company announced it has been granted two Rare Pediatric Disease Designations (RPDD) by the United States Food and Drug Administration (FDA) for the treatment of GM1 and GM2 gangliosidoses, Orphan Medicinal Product Designation (OMPD) by the European Medicines Agency (EMA) for the treatment of GM2 gangliosidosis, and an Innovation Passport by the UK Medicines and Healthcare Products Regulatory Agency (MHRA) for the treatment of GM1 and GM2 gangliosidoses.GRO BiosciencesGRO Biosciences is leveraging groundbreaking science to expand the amino acid alphabet and deliver on protein therapeutics. The U.S. company is transforming treatments for increasingly prevalent chronic medical conditions including autoimmune and metabolic diseases to improve the lives of patients. GRObio is applying its platform to advance partnered and collaborative programs, as well as its own pipeline of protein therapeutics bearing unique NSAA (non-standard amino acid) chemistries. The company's NSAA therapeutics feature previously unattainable capabilities including unprecedented duration of action and precise regulation of the immune system.

Framtidens E-Handel
144. Hanna Eiderbrant: Principal på Verdane- Om e-handelsinvesteringar, risker med influencer brands, likviditet, och när en e-handel är oinvesterbar

Framtidens E-Handel

Play Episode Listen Later Jan 20, 2023 55:22


Verdanes Hanna Eiderbrant gästar podden Framtidens E-Handel och delar med sig av sina kunskaper inom investering.10:30 min - Vad gör och är Verdane Capital, och vad är Hannas roll?19:10 min - Vilka risker finns med influencer brands och hur ser investerare på dessa riskfaktorer? Vilka benchmarks vill man se i GM1, 2 & 3?32:20 min - Vilka kategorier är mer och mindre intressanta nu inom D2C?38:30 min - Snackar cash flow.46:45 min - När blir en e-handel oinvesterbar?Här hittar du Hanna & Verdane:https://www.linkedin.com/in/eiderbrant/?originalSubdomain=se https://verdane.com/ Följ Björn på LinkedInhttps://www.linkedin.com/in/bjornspenger/ Följ Framtidens E-handel på LinkedInhttps://www.linkedin.com/company/framtidens-e-handel/ Besök vår hemsida & Instagramhttps://www.framtidensehandel.se/ https://www.instagram.com/framtidens.ehandel/ Sponsorhttps://www.juni.co/framtidens Tusen tack för att du lyssnar!Support till showen http://supporter.acast.com/framtidens-e-handel. Hosted on Acast. See acast.com/privacy for more information.

The 365 Days of Astronomy, the daily podcast of the International Year of Astronomy 2009
Travelers in the Night EPs. 639 & 640: Earth Approachers & Rocks or Rockets

The 365 Days of Astronomy, the daily podcast of the International Year of Astronomy 2009

Play Episode Listen Later Jan 15, 2023 5:30


Dr. Al Grauer hosts. Dr. Albert D. Grauer ( @Nmcanopus ) is an observational asteroid hunting astronomer. Dr. Grauer retired from the University of Arkansas at Little Rock in 2006. travelersinthenight.org Today's 2 topics: - During a recent 60 day period asteroid hunters discovered 29 space rocks as they passed closer to us than the Moon.  - Kacper Wierzchos discovered 2021 GM1 in the constellation of Virgo. It might be a Moon rock or a piece of Apollo mission debris.   We've added a new way to donate to 365 Days of Astronomy to support editing, hosting, and production costs.  Just visit: https://www.patreon.com/365DaysOfAstronomy and donate as much as you can! Share the podcast with your friends and send the Patreon link to them too!  Every bit helps! Thank you! ------------------------------------ Do go visit http://www.redbubble.com/people/CosmoQuestX/shop for cool Astronomy Cast and CosmoQuest t-shirts, coffee mugs and other awesomeness! http://cosmoquest.org/Donate This show is made possible through your donations.  Thank you! (Haven't donated? It's not too late! Just click!) ------------------------------------ The 365 Days of Astronomy Podcast is produced by the Planetary Science Institute. http://www.psi.edu Visit us on the web at 365DaysOfAstronomy.org or email us at info@365DaysOfAstronomy.org.

Morning Woodward Show
Morning Woodward Show | Thursday, October 20th, 2022

Morning Woodward Show

Play Episode Listen Later Oct 20, 2022 119:23


Adham Beydoun and Jeff Iafrate react to the Detroit Pistons Opening Night win against the Orlando Magic, discuss the impressive debuts for Jaden Ivey and Jalen Duren and so much more!#DetroitPistons #DetroitLions #WoodwardSports #OnePride #Pistons #Lions #LGRW 00:00 Pistons Def. Magic 113-109: Ivey & Duren SHINE in Debut15:50 Ivey & Duren's Impressive Debuts - How Good Can They Be?32:45 Dak Prescott Cleared to Play - Can Lions Take Advantage? 45:40 Looking Back at What Brad Holmes has Done as GM1:01:10 Pistons Young Core Shines - Early Takeaways, Future Potential1:13:30 MicroMike Interview1:36:00 Jakub Vrána Entering Player Assistance Program1:41:20 TNF Preview: Saints vs. Cardinals1:52:00 MailbagWatch Our Shows Live on YouTube 7 Days a Week!Morning Woodward Show | Monday-Friday 8am-10am Big D Energy | Monday-Friday 11am-1pmErmanni and Edwards | Monday-Friday 2pm-4pmWoodward Heavyweights | Monday-Friday 5pm-7pmFollow The Woodward Sports Network!Twitter: https://twitter.com/woodwardsportsTikTok: https://www.tiktok.com/@woodwardsport...Instagram: https://www.instagram.com/woodwardspo...Facebook: https://www.facebook.com/WoodwardSports/Download Our App for Apple: https://apps.apple.com/us/app/wsn-liv...Download Our App for Android: https://play.google.com/store/apps/de...

Lets Go Blues Radio - St. Louis Blues Hockey Podcast
Se10, Ep39: 2022 Rd2 Gm1 Blues @ Avalanche - Breakdown

Lets Go Blues Radio - St. Louis Blues Hockey Podcast

Play Episode Listen Later May 19, 2022 96:34


RockinThatIDLife.com - For 10% OFF, Email Dustin at rockinthatidlife@gmail.com & tell him "Lets Go Blues sent me!" CenterIceBrewery.com - St. Louis brewed hockey-themed beer! Join our Discord Server! www.LetsGoBlues.com/discord Beers of the Show (abbreviated) Follow us on the UnTapped Beer App cprice12 jponder94 billybluenote33 Today in Blues History Curt, Bill & Jeff break down Gm1 of the 2nd rd matchup between the St. Louis Blues & Colorado Avalanche. Dom Leschychyn from The Athletic is a bit of a douche and we talk about him and his computer prediction "models" Rapid Fire Tid Bits From Around the NHL: The Battle for Florida The Battle for Alberta The Battle of... The East Coast? Arizona's Center Ice Logo... LOL  We interact with our live listeners! AND MORE!

Taproom Sports Podcast
094: 5/18/22- NBA EAST FINALS GM 1 RECAP, NHL CSF GM 1 RECAPS + MORE!

Taproom Sports Podcast

Play Episode Listen Later May 18, 2022 45:03


Ben and Jordan are recapping the Eastern Conference Finals Gm 1 between the Heat and the Celtics, and looking ahead to the WCF GM 1 between the Golden State Warriors and Dallas Mavericks. Plus they're recapping Gm1's of the NHL Conference semi's; Lightning vs Panthers, and Avs vs Blues and looking ahead to tomorrow's Game 1 between Oilers and Flames. They close giving you their best bets of the day for Wednesday 5/18/22! Brought to you by Tavour. Go to www.TAVOUR.COM and use promo code: TAPROOM for $10 off your first purchase of $25 or more! TAPROOMSPORTSPODCAST.COM

RBLR Sports
RBLR Lightning: (RD1) Bolts & Leafs Tied 1-1

RBLR Sports

Play Episode Listen Later May 5, 2022 43:15


What a series already! The road Bolts did the job and split the series and now have home-ice advantage. TOR took advantage of a bad GM1, but our boys turned it around in GM2. We talk the ups and downs, and what to expect next. #gobolts #boltsnation Support The Pod! Buy A Shirt! Use Promo […]

Vipers on RBLR Sports
RBLR Lightning: (RD1) Bolts & Leafs Tied 1-1

Vipers on RBLR Sports

Play Episode Listen Later May 5, 2022 43:15


What a series already! The road Bolts did the job and split the series and now have home-ice advantage. TOR took advantage of a bad GM1, but our boys turned it around in GM2. We talk the ups and downs, and what to expect next. #gobolts #boltsnation Support The Pod! Buy A Shirt! Use Promo […]

Hypermobility Happy Hour
40 - Exciting Research on EDS in Animals: Dr. Heather Gray-Edwards and Dr. Abby McElroy

Hypermobility Happy Hour

Play Episode Listen Later Mar 23, 2022 36:18


On this episode, we have returning guest Dr. Abby McElroy who previously was a guest on the Hypermobility Happy Hour discussing EDS in animals. Today, she returns with her principal investigator Dr. Heather Gray-Edwards to discuss the exciting new research they have been working on. Dr. Gray-Edwards is an assistant professor of Radiology at the University of Massachusetts' Chan Medical School and is a member of the Horae Gene Therapy Center. Much of her research has been focused on evaluating therapeutic interventions in testing novel Adeno-associated viral vectors and delivery routes using both biochemical and molecular biology techniques, as well as in vitro evaluation like neurological examinations, MRIs, and electrodiagnostics of GM1 and GM2 gangliosidosis cats. Dr. McElroy is a doctoral student who previously received her Doctor of Veterinary Medicine degree from Tufts University in 2017. She further obtained a master's degree from Michigan State University in 2018 which focused on the characterization of neurological abnormalities in an equine model of Ehlers-Danlos syndrome. From 2018 to 2021, Dr. McElroy was a Postdoctoral Research Associate in the Department of Neurosurgery at Rhode Island Hospital and the Medical School of Brown University where she studied tethered cord syndrome and myodural bridge dysfunction in patients with Ehlers-Danlos Syndrome. In her current position at the Gray-Edwards laboratory at the University of Massachusetts' Chan Medical School focuses on the development of gene therapy for Ehlers-Danlos syndromes and multisystemic smooth muscle dysfunction syndrome (related to the ACTA2 mutation). Abby also has two cats and two dogs and one of her cats has Ehlers-Danlos syndrome and is a bit of a social media star in his own right (his name is Reed and you can follow him at reed_eds)

RBLR Sports
RBLR Rays: ALDS Tied 1-1

RBLR Sports

Play Episode Listen Later Oct 9, 2021 32:02


The Rays are fine. Time for some group therapy. The ALDS is now tied at 1-1 after two games, a wonderful overall performance in GM1, and an explosion by BOS in GM2. Randy stole home! Luplow hit a Grand Slam! Playoff baseball is awesome & frustrating. Let's talk about it! #relentless #raysup Spotify: RBLR Sports […]

Le Journal des Biotechs
Le JDB : Karen Aiach (Lysogene), Jamila El Bougrini (Invest Securities)

Le Journal des Biotechs

Play Episode Listen Later Jun 16, 2021 31:26


Dans ce numéro du Journal des biotechs, on passe en revue les dossiers chauds du moment avec Jamila El Bourgrini, analyste biotechs chez Invest Securities : AB Science, intros en Bourse, traitement dans Alzheimer... L'entretien est consacré à Karen Aiach, fondatrice et directrice générale de Lysogene. Elle revient sur les annonces récentes de la société, notamment l'accord de licence avec SATT Conectus et le premier patient traité avec LYS-GM101 dans la Gangliosidose à GM1.

RARECast
Targeting CNS Disease with Gene Therapies

RARECast

Play Episode Listen Later Jun 3, 2021 40:40


The translational challenges of moving an experimental therapy from the lab to the clinic can stall the development of life-saving therapies, but Passage Bio has provided a unique solution to that challenge. The company's strategic collaboration and licensing agreement with the University of Pennsylvania's Gene Therapy Program leaves the discovery and preclinical work in the hands of Penn researchers and provides it with enhanced access to a broad portfolio of gene therapy candidates and future innovations. The company has built a pipeline of gene therapy candidates targeted central nervous system disorders. We spoke to Bruce Goldsmith, CEO of Passage Bio, about the company's relationship with Penn's Gene Therapy Program, its focus on CNS conditions, and the company's lead program in the rare lysosomal storage disorder GM1 gangliosidosis.

Business Of Biotech
AAV & Lentivirus Gene Therapies with Sio Gene Therapeutics' Dr. Pavan Cheruvu

Business Of Biotech

Play Episode Play 57 sec Highlight Listen Later May 24, 2021 57:21


Fresh on the heels of a significant data readout on its AXO-AAV-GM1 candidate at the recent ASGCT meeting, Sio Gene Therapies CEO Pavan Cheruvu, M.D. joins the Business of Biotech podcast.  We discuss Dr. Cheruvu's formative years and inspiration, his company's origins, its progress developing AAV and lentiviral gene therapies to treat GM1 gangliosidosis, Tay-Sachs / Sandhoff disease, and Parkinson's disease, and the challenges the company has overcome in a hybrid manufacturing environment that will ultimately transfer entirely in-house. 

Shooters Roll
Orlando bubble NBA finals 2020 LAL VS MIA - Shooters’ Roll Ep42

Shooters Roll

Play Episode Listen Later Oct 1, 2020 35:12


Lloyd hosts the latest episode of Shooters' Roll. 1:05 GM 1 NBA Finals LAL Vs MIA Dex's analytics 1:15, 3:04 RJ's analytics 4:27 Tee's analytics of the game 5:30 Lloyds analytics of GM1 6:41 Dex's opinion on lineup changes/ injuries for the heat 9:17 Tees opinion on lineup / injury changes 10:18 Rj's take on lineup / injury changes for MIA 12:10 Lloyds take on the lakers mamba themed jerseys 12:32 Dex's take on the mamba uniforms/ MIA strategy going forward 14:32 Lloyd/ crew discusses about Katya Henry the ex vs the current 16:51 Lloyd discusses Docs firing / new coach 17:19 Tees take on LAC coaching job 17:54 Dex's take on LAC coaching job 20:15 Rj's take on LAC coaching job 20:53 Lloyd/the crew discusses Nba rumours /potential trades/ transactions 26:14 Dex reminisces /crew discusses about Linsanity 28:08 The crew discusses GM2 31:58 The crew discusses potential Finals MVP --- Send in a voice message: https://anchor.fm/shootersroll/message

Shot of Sports
Florida Boys

Shot of Sports

Play Episode Listen Later Sep 30, 2020 64:27


S1E3 Dre and Rony featured their "Liquor of the week " The fireball Cherry Apple Bomb. Paying tribute to the red hot Miami Heat making it to the NBA Finals in Pursuit to their 4th NBA Title in franchise history. The guys talk about GM1 finals Heat Vs Lakers predictions and NFL week 4 recap. Leave feedback to improve further episodes! http://anchor.fm/shotofsports --Florida Boys-- iP S1E3 --------------------------------------------------------- Connect with us on all the things Instagram: @shotofsports Podcast: http://anchor.fm/shotofsports --- Send in a voice message: https://podcasters.spotify.com/pod/show/shotofsports/message

Kinner & Schlemmer
The Justin Kinner Show w/Kev Nash! (FULL SHOW 9/22/20)

Kinner & Schlemmer

Play Episode Listen Later Sep 24, 2020 120:38


The Justin Kinner Show w/Kev Nash! - The Reds get a huge win over Brewers in GM1 and move up in the playoff standings - Ohio State's 2020 season schedule has set them up to cruise into the College Football Playoff - ESPN Radio's Jason Fitz hops on the show to talk CFB & NFL headlines

DosisdeInternas
INFECTOLOGÍA: Cólera

DosisdeInternas

Play Episode Listen Later Sep 24, 2020 10:04


Las enterotoxina se unen al GM1 y se activa la adenilatociclasa causando una diarrea secretora (como agua de lavado de arroz)..... lee el tema antes y luego escucha el audio completo

W.I.S.E. Wisdom in Sports and Entertainment
W.I.S.E. Episode 3 NBA 2020 WCF Lakers vs. Nuggets GM2 Recap, GM3 Preview

W.I.S.E. Wisdom in Sports and Entertainment

Play Episode Listen Later Sep 22, 2020 59:13


Hello Listeners! Skip A. Smith and I, Bob Einstein, are BACK...back again.... Skip and Bob are back.... tell a friend.... We idiots will be discussing GM1 of the 2020 WCF and previewing GM2. The breakdown for each team will be the following: 1. Offensive Adjustments 2. Defensive Adjustments 3. Mindsets Join in to hear our dorky insights into this exciting playoff match up! Thank you for listening! As always, Stay Wise. Stay Silly.

W.I.S.E. Wisdom in Sports and Entertainment
W.I.S.E. Episode 2 NBA 2020 WCF Lakers vs. Nuggets GM1 Recap and GM2 Preview

W.I.S.E. Wisdom in Sports and Entertainment

Play Episode Listen Later Sep 21, 2020 43:18


Hello Listeners! Skip A. Smith and I, Bob Einstein, are BACK (to the future...)! We idiots will be discussing GM1 of the 2020 WCF and previewing GM2. The breakdown for each team will be the following: 1. Offensive Adjustments 2. Defensive Adjustments 3. Mindsets Join in to hear our dorky insights into this exciting playoff match up! Thank you for listening! As always, Stay Wise. Stay Silly.

TABLETOP TALK - A Third Floor War's Podcast
Insider Insight Through the Breach RPG with Dead Aussie Gamer - ep. 84

TABLETOP TALK - A Third Floor War's Podcast

Play Episode Listen Later Jul 28, 2020 94:36


Join Craig as he talks to Michael Kesavan (Dead Aussie Gamer) about Wyrd's Through the Breach roleplaying system. They discuss what makes it great and unique, how to be a better player (and GM1), and what the plans are for the 2020 Gencon events. Check out Michael on Twitch: www.twitch.tv/deadaussiegamer and on YouTube: www.youtube.com/deadaussiegamer https://www.youtube.com/playlist?list=PLWqG_tm-1K8qnUotLMzEh9i3m2HGo-PvR Support us on Patreon!: https://www.patreon.com/Thirdfloorwars The best tokens are at Customeeple.com - use the promo code ThirdFloorFriend for a 5% discount on everything except retail products and playmat. Support us (and get the best mats in the business) at Mats by Mars and get 10% off your entire order by using the promo code: THIRDFLOOR920 https://matsbymars.com/collections/36-x-36-mats Get a cool T-Shirt or mug and help us bring you more content. The store is open! https://thirdfloorwars.com/shop/ Support your favorite podcast and get some killer Malifaux gadgets here: https://www.etsy.com/listing/687047710/third-floor-wars-malifaux-accessory-set?ref=hp_rf-1 Support us (and get free shipping on orders over $100) at Gadzooks Gaming: https://www.gadzooksgaming.com Follow us on Facebook: https://www.facebook.com/thirdfloorwars/ Follow us on Twitter: @ThirdFloorWars Watch our videos: https://www.youtube.com/channel/UCA496705JLkpgAssAhetpdw Give us feedback or leave us a message that we can use in a future episode! You can record your message here: https://anchor.fm/thirdfloorwars/message You can also record your question as an audio file and email it to us admin@thirdfloorwars.com --- Send in a voice message: https://anchor.fm/thirdfloorwars/message Support this podcast: https://anchor.fm/thirdfloorwars/support

PaperPlayer biorxiv biophysics
3D-Visualization of Amyloid-β Oligomer and Fibril Interactions with Lipid Membranes by Cryo-Electron Tomography

PaperPlayer biorxiv biophysics

Play Episode Listen Later Jul 21, 2020


Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2020.07.21.214072v1?rss=1 Authors: Tian, Y., Liang, R., Kumar, A., Szwedziak, P., Viles, J. H. Abstract: Amyloid-{beta} (A{beta}) monomers assemble into mature fibrils via a range of metastable oligomeric and protofibrillar intermediates. These A{beta} assemblies have been shown to bind to lipid bilayers. This can disrupt membrane integrity and cause a loss of cellular homeostasis, that triggers a cascade of events leading to Alzheimers disease. However, molecular mechanisms of A{beta} cytotoxicity and how the different assembly forms interact with the membrane remain enigmatic. Here we use cryo-electron tomography (cryoET) to obtain three-dimensional nano-scale images of various A{beta} assembly types and their interaction with liposomes. A{beta} oligomers bind extensively to the lipid vesicles, inserting and carpeting the upper-leaflet of the bilayer. Furthermore, curvilinear protofibrils also insert into the bilayer, orthogonally to the membrane surface. A{beta} oligomers concentrate at the interface of vesicles and form a network of A{beta}-linked liposomes. While crucially, monomeric and fibrillar A{beta} have relatively little impact on the membrane. Changes to lipid membrane composition highlights a significant role for GM1-ganglioside in promoting A{beta}-membrane interactions. The different effects of A{beta} assembly forms observed align with the highlighted cytotoxicity reported for A{beta} oligomers. The wide-scale incorporation of A{beta} oligomers and curvilinear protofibrils into the lipid bilayer suggests a mechanism by which membrane integrity is lost. Copy rights belong to original authors. Visit the link for more info

The Roundup Podcast
447: On the Couch - All Around the NFL: Week 8 Previews & Predictions, Plus NBA & MLB, with the Guru, Gabe Goldfield - The Roundup Podcast

The Roundup Podcast

Play Episode Listen Later Oct 25, 2019 95:09


Now, back to our regularly scheduled programming. It's been a wild week in the sports world, with the NFL season rolling along, MLB Playoffs in full swing, and in order to properly welcome the NBA back into our lives, we had to put out a special bonus podcast to fit it all in! It's a sports fan's favorite time of year, the annual convergence of the Big Three of American sports, and this Sunday marks a special day on the sports calendar, what I like to call the sports eclipse, where we get a full slate of NFL games, a World Series game, as well as multiple NBA games to watch. In the NFL, good matchups are few and far between, but Panthers/Niners could be good, and Browns/Pats will likely draw some ratings. In the Series, we should, if it's not a Nationals' sweep, get a rematch of GM1's battle between aces Gerrit Cole and Max Scherzer, always a possibility to see a historical postseason pitching duel. And in the NBA, it's my Warriors visiting OKC, and HalleLuka, my boy Luka Doncic vs the Blazers, so I wouldn't be surprised if it was early season hoops dominating the main screen in my house that night. If you want find me Sunday, on the couch is your best bet. The sports eclipse is rare, it's beautiful, and unlike an actual eclipse, I'm actually encouraging everyone to watch. So sit back, the NFL season nears the midway point, the NBA is back, the Nationals are dominating the World Series and we're breaking down how it's all unfolding, next on the Roundup Podcast, so let's get rolling . . .

The Best in Sports News and Analysis, from Danielle McCartan

WFAN Sunday, October 13, 2019 3am-6am Twitter: @CoachMcCartan www.facebook.com/CoachMcCartan website: www.prosportsrundown.com Guest: Jim Leyrtiz Producer: Kevin Wall :00 Opening Monologue #Yankees: “Feel this Moment” Pitbull feat. Christina Aguilera #Giants: “Everything's Gonna Be Alright” David Lee Murphy & Kenny Chesney #Jets: “Hard to Love” Lee Brice #Mets: “Who Are You?” The Who 15:35 NYY NEEDED to win Game 1, plan for Game 2, #McCartanMath, calls, Tanaka in the 7th, NYY rotation, CC/Hicks for Voit/Wade 28:00 #McCartanMath: Paxton vs. Verlander, pitching matchups, DJ LaMaheiu my breakout star for GM 2, NYY domination good for baseball? NJ Shop Rite has PATS napkins 38:24 So why did Boone yank Tanaka?, calls, Darnold running for his life, CC on roster, starting SS on my varsity team, Nationals 52:00 Defense on display in ALCS 56:13 Jets vs. Cowboys preview & prediction, Falk waived?! , Gase's coaching blunders, 1:02:00 3 reasons why Gase needs to be relieved of duties ASAP, debunking excuses 1:06:28 Exclusive interview with WS winning Jim Leyritz: GM1 recap, GM2 preview, former teammate Girardi a good fit for Mets?, his career in pinstripes 1:22:04 1:23:45 GM1 recap, GM2 look ahead, NYY rotation, Tanaka in the 7th?, CC/Hicks, Torres, on roster 1:28:30 NYM managerial search update, Cano contract implications 1:32:45 2019 Jets and beyond, 5 keys for the Jets to (maybe.. probably not) beat the Cowboys , score prediction 1:45:00 NYY vs HOU team batting, pitching, relief pitching

The 365 Days of Astronomy, the daily podcast of the International Year of Astronomy 2009
Travelers in the Night Eps. 37 & 38: Slow Movers At The Edge & The Big Ones

The 365 Days of Astronomy, the daily podcast of the International Year of Astronomy 2009

Play Episode Listen Later Sep 29, 2019 6:20


Dr. Al Grauer hosts. Dr. Albert D. Grauer ( @Nmcanopus ) is an observational asteroid hunting astronomer. Dr. Grauer retired from the University of Arkansas at Little Rock in 2006. travelersinthenight.org Today's 2 topics: - Imagine a world that is so far from our Sun that it receives less than 1/5,000 of the heat and light that makes our life possible. If you were standing on it, you could cover the Sun with the tip of a ball point pen held at arms length. - Dr. Grauer discovered 2014 GM1, the second Km sized near Earth asteroid to be identified in 2014, while observing with the Catalina Sky Survey telescope on Mt. Lemmon in Arizona.   We've added a new way to donate to 365 Days of Astronomy to support editing, hosting, and production costs. Just visit: https://www.patreon.com/365DaysOfAstronomy and donate as much as you can! Share the podcast with your friends and send the Patreon link to them too! Every bit helps! Thank you! ------------------------------------ Do go visit http://astrogear.spreadshirt.com/ for cool Astronomy Cast and CosmoQuest t-shirts, coffee mugs and other awesomeness! http://cosmoquest.org/Donate This show is made possible through your donations. Thank you! (Haven't donated? It's not too late! Just click!) The 365 Days of Astronomy Podcast is produced by Astrosphere New Media. http://www.astrosphere.org/ Visit us on the web at 365DaysOfAstronomy.org or email us at info@365DaysOfAstronomy.org.

Lets Go Blues Radio - St. Louis Blues Hockey Podcast
Se7, Ep50: Games 1 & 2 - Stanley Cup Final - STL vs BOS

Lets Go Blues Radio - St. Louis Blues Hockey Podcast

Play Episode Listen Later May 31, 2019 124:52


The St. Louis Blues win their first ever Stanley Cup Final game! We talk about that, along with the 4-2 loss in Gm1, St. Louis watch parties, the officiating, Krug's hit on Thomas, Sundqvist gets suspended for 1 game, the disgrace that is Bar Stoolsports & their sponsorship of Boston's rally towels, plus our Beers of the Week, Today in Blues History, and much more in this jam packed episode!

Kinner & Schlemmer
Fired up #Reds fan defends Jesse Winker during Kinner & Schlemmer Show

Kinner & Schlemmer

Play Episode Listen Later May 3, 2019 2:00


Jesse Winker is the Reds HR leader at the moment with 8 and he has 13 RBI's on top of that - Winker is one of the biggest fan favorites on the roster at the moment for his memorable game winning HR vs the Metz in GM1 and his sliding diving catch to end the game and clinch a W vs the Metz in GM3. Schlemmer is still not sold on Winker as the team's HR leader is only hitting .224 and a caller called in fired up and ready to defend him.

Kinner & Schlemmer
Kinner & Schlemmer react to the Reds GM1 loss to the Dodgers

Kinner & Schlemmer

Play Episode Listen Later Apr 16, 2019 9:56


Reds blow a tough one in GM1 vs the Dodgers - Reason to be concerned over Rasiel Iglesias's continued mound struggles? The guys discuss on the Kinner & Schlemmer Show

AggroChat: Tales of the Aggronaut Podcast
AggroChat #244 - Loot and Item Scaling

AggroChat: Tales of the Aggronaut Podcast

Play Episode Listen Later Mar 17, 2019 63:38


Tonight signals the triumphant return of Doctor Grace!  I cannot adequately express how happy we are to have her back among our number.  As far as topics go… we once again punt forward the only topic that we had planned and replace them with a few discussions gathered up moments before we started recording.  Tonight we talk about Anthem and get Grace’s impressions so far… as well as dig into some of the frustrations around high end loot and weird item scaling. Thalen tells us how to learn to love Eureka in Final Fantasy XIV and get past the fact that it is so grindy.  Finally we wrap things up with a discussion about the Super Mario World Rom Hack scene and the differences between Vanilla and Chocolate. Topics Discussed The Return of Doctor Grace Anthem Grace and Thalen Impressions High End Loot Woes Item Scaling Weirdness Final Fantasy XIV Eureka Grinding Relic Weapons Super Mario World Rom Hacks Kaizo Mario Terminus Assembly Editing

Bang Average
Week 2 Preview: KDB Injured Yo

Bang Average

Play Episode Listen Later Aug 17, 2018 69:55


The fella's break down the big KDB injury news with Ben Dinnery, talk replacements and who benefits in City's allstar team. There's also analysis of the rest of GM1's action and a slate of listener questions, including the long awaited 'Carmen's Question of the Week' jingle.

Convos with Cutty
Post Spurs/Warriors GM1 with TheWordisMarc

Convos with Cutty

Play Episode Listen Later Apr 14, 2018 11:48


We discuss GM1 between the Spurs/Warriors with TheWordisMarc.

Rob Dibble Show
CASEY STERN ON WS GM1

Rob Dibble Show

Play Episode Listen Later Oct 26, 2017 14:37


Stern gives us the up close look from LA at Gm1. Temp is above 100 degrees.

Celtics Post Game - Powered by BetOnline
GM1: Cavs @ Boston Celtics | 2016-17 NBA Playoffs, EC Finals | LeBron v Isaiah

Celtics Post Game - Powered by BetOnline

Play Episode Listen Later May 18, 2017 176:00


CLNS Radio brings you game 1 of the Boston Celtics v LeBron James and the Cleveland Cavaliers, NBA EC Finals Series, brought to you by It's playoffs season and having the right players on the court will be the key to success.  That sharp outside shooter or power rebounder can be the difference between winning and losing. Business isn't any different. Your company needs the RIGHT people to be the best. So where do you go to find top talent? At ZipRecruiter.com, you can post your job to 100+ job sites with one click. Then, their powerful technology efficiently matches the RIGHT people to your position. ?And right now my listeners can start forming their own winning team on ZipRecruiter for FREE! Just go to ZipRecruiter.com/sportsfan. Try it for free today at ZipRecruiter.com/sportsfan” The Celtics Post Game Show will go LIVE on CLNS Radio, following the Celtics and the Washington Cavs, game 1. Make sure you return to CLNS Radio after the game to get the immediate reactions. Patrick Gilroy and Nic S. have the hosting honors for GM1. The guys will be taking your TOLL FREE calls throughout at 347-215-7771.

Fearless Parent Radio
Ketogenic Diets for Kids with Cancer - 02.11.15

Fearless Parent Radio

Play Episode Listen Later Feb 12, 2015 57:27


Guest // Thomas Seyfried, PhDHost // Toni Bark, MDHalf of men and one-third of women will receive a cancer diagnosis sometime during their lives. Increasingly, more patients are declining conventional treatment. They are interested in a different kind of approach to cancer management and prevention.. one that is more cutting edge and incorporates the latest research.If we can understand what cancer is, what feeds it, and why metastasis happens, then we can find and apply more effective strategies to shrink (and hopefully eliminate) tumors.Meet Professor Thomas Seyfried. He is a biochemical geneticist who has been investigating the lipid biochemistry of cancer for thirty years. His pioneering research expands on Otto Warburg’s groundbreaking theory that cancer is a disease of energy metabolism. With this framework, he’s able to explain the relationship between cancer and inflammation, vascularization, cell death, drug resistance, and genomic instability. Discussion topics during today’s show include:How does this view differ from the direction in which much of current research and funding is heading? (i.e., genetics)Is the controversy largely a matter of cause and effect (i.e., mitochondrial respiration and genetic damage)?What has been the response of the scientific community?Where is the research heading? (i.e., mouse to man, clinical trials?)What is the ketogenic diet and how is it implemented? How does this differ when applied to children?What is the Press-Pulse metabolic strategy for cancer management?If you have questions, be sure to send them before the show or meet up with us on the blog afterwards to discuss.Thomas Seyfried, PhD focuses on gene environmental interactions related to complex diseases, such as epilepsy, autism, brain cancer, and neurodegenerative (the GM1 and GM2 gangliosidoses) diseases. He investigates many of these diseases from the perspective of, genetics, lipidomics, and energy metabolism. Tom received his Ph.D. in Genetics and Biochemistry from the University of Illinois, Urbana, in 1976. He  was an Assistant Professor in Neurology at Yale University School of Medicine. Tom has received awards from the NIH, The American Society for Neurochemistry, and the Ketogenic Diet Special Interest Group of the American Epilepsy Society. He was Chair, Scientific Advisory Committee for the National Tay-Sachs and Allied Diseases Association and serves on several editorial boards, for Nutrition & Metabolism, Neurochemical Research, the Journal of Lipid Research, and ASN Neuro. Read more about his work here.

Fakultät für Physik - Digitale Hochschulschriften der LMU - Teil 02/05
Structure, Fluidity and Phase Behavior of Supported Lipid Membranes: An Investigation by X-ray Reflectivity and Fluorescence Microscopy

Fakultät für Physik - Digitale Hochschulschriften der LMU - Teil 02/05

Play Episode Listen Later Jul 31, 2007


The structure of mammalian cell membranes is highly heterogeneous and consists of numerous lipid and protein molecules, which are organized into the cellular lipid bilayer. Understanding membrane processes such as lipid-protein interactions requires an insight into the molecular structure of the cell membrane. Such ångstrøm resolution is offered by X-ray diffraction techniques, which are sensitive to the electron density distribution within macromolecules. Model lipid membranes mimic the composition of natural cell membranes and are used for facilitating experimental investigations. A special class of biomimetic lipid membranes are substrate supported lipid bilayers, which can be studied by surface sensitive methods such as X-ray reflectivity. Using highly brilliant X-rays at modern synchrotron sources allows to obtain detailed structural information on lipid bilayers at solid-liquid interfaces. For this thesis, a novel microfluidic setup for high resolution X-ray reflectivity studies of single biomimetic lipid membranes at solid-liquid interfaces was developed. The setup is also designed for quantitative fluorescence microscopy, which allows us to complement our structural studies with investigations on lipid dynamics within the lipid bilayer. Our approach unifies two experimental characterization techniques on a single sample and offers an integrated view on the biophysical properties of biomimetic lipid membranes, such as molecular structure, lipid fluidity and phase state of the lipid bilayer. We have characterized lipid bilayers on different solid supports to assess the suitability of these membrane/interface systems for biological and biotechnological applications. The surface chemistry of an underlying substrate may considerably influence the structural and dynamical properties of a lipid membrane. The material properties of the thermoplastic polymer 2-norbornene ethylene (Topas), such as optical transparency, high chemical resistivity and ease for lateral structuring, make this compound an interesting candidate as a substrate for lipid membranes. Model lipid bilayers on Topas showed a high homogeneity, though a reduced lipid fluidity (~50%) as compared to lipid bilayers supported on hydrophilic silicon oxide. We also observed on Topas a reduced bilayer thickness of about 20%, which we ascribe to a bilayer conformation with either coiled or interdigitated acyl chains. Another template for biosensoric applications are polyelectrolyte multilayers, which can act as a dielectric between lipid bilayers and semiconductor substrates, such as silicon-on-insulator devices (SOI). We studied homogeneous lipid bilayers on alternating polyanion/polycation layers and characterized the corrugation of the bilayer depending on the number of underlying polyelectrolyte layers. Further, we studied how protein and receptor molecules bound to lipid membranes influence their structure and lipid fluidity. The binding of the protein streptavidin to biotin molecules has a strong noncovalent affinity and is widely used in biotechnological research. We characterized the formation of a streptavidin/avidin layer bound to a supported lipid bilayer containing biotinylated lipids. We resolved a well-defined water layer of 8Å separating the protein and lipid bilayer and showed that the bilayer structure was not affected by the presence of the protein. The lipid fluidity was quantified using continuous bleaching before and after protein binding and we observed a small reduction of 10-15% of the lipid diffusion constant after protein binding. We propose that the separating water layer allows the lipid bilayer to retain its lateral fluidity and structural integrity. Finally, we studied biomimetic membranes with complex mixtures that approximate the lipid composition in mammalian cell membranes. Such lipid membranes with multiple components including cholesterol are capable of phase separation into condensed and non-condensed lipid phases. Condensed lipid domains are more ordered than their environment and localize membrane receptors. We studied the membrane receptor GM1 ganglioside in supported lipid bilayers of ternary compositions including cholesterol and observed membrane condensation, which was induced by the presence of the receptor. Using the high structural resolution available with synchrotron reflectivity, we determined that this receptor-induced condensation can be asymmetric and is restricted to the bilayer leaflet in which GM1 is present. The membrane fluidity was significantly reduced (~50%) by the presence of GM1 and we observed lateral segregation into microscopic domains (~5µm) with fluorescence microscopy. In this thesis, complementary experimental techniques were applied to investigate the ångstrøm scale structure and diffusion properties of biomimetic lipid membranes. We systematically studied how substrate chemistry, lipid-bound macromolecules and lipid ordering influence the structure and fluidity of lipid bilayers. The present microfluidic setup can be used to study other complex lipid membrane systems to improve our physical understanding of lipid membrane interfaces.