Podcasts about nscs

Schwarze Katze von rechts: Pech. Schwarze Katze von links: Glück. Schwarze Katze in altem Wald bei D&D: 3 Antworten. In dieser Folge stellen wir euch die glücks- und unglücksbringenden Nichnys und ihre Besonderheiten vor. Sollte euch die Folge zu lang sein oder ihr euch nur für einzelne Teile interessieren, findet ihr hier die Kapitelmarker für die gesamte Folge: (01:42) Trims Geschichte (08:43) Nichnys außerhalb von D&D (09:16) Nichnys in D&D (11:22) Physische Merkmale von Nichnys (13:21) Lebensweise der Nichnys (28:55) Spielwerte von Nichnys (35:44) (Kampf)Taktiken von Nichnys (55:20) Nichny-NSC: Oropurr Download der NSC-Tabelle und von Oropurrs Spielwerten: ⁠⁠dndbpod.webador.de/dokumente⁠⁠ Portraits und Spielwerte der NSCs aus unseren anderen Folgen findet ihr auch auf unserem ⁠⁠Instagram⁠⁠-Account. Wenn ihr uns unterstützen wollt, könnt ihr das auf ⁠⁠PayPal⁠⁠ oder ⁠⁠BuyMeACoffee⁠⁠ machen! Vielen Dank an Kobold Press für die Erlaubnis diese spannende Folge machen zu dürfen. Schaut euch ⁠Tome of Beasts⁠ und andere tolle Bücher auf der Webseite von Kobold Press an. Tome of Beasts wurde von Ulisses auf Deutsch übersetzt und ist ⁠hier⁠ erhältlich. Es handelt sich um eine ehrliche Empfehlung und nicht um bezahlte Werbung. 

Tonight's show will be broken into two halves. Our first Guest WeatherBrain is a retired Army noncommissioned officer with a background in special operations/weather.  He currently does some work for streaming service WeatherNation as a field meteorologist.  He's also a part time police officer as well as Founder/Director of the National Storm Chaser Summit.  Erik Fox, it's an honor to have you join us for this first show of 2024. The 2nd half of our inaugural 2024 show focuses on auroras and features a professional aurora chaser.  He's a photographer and a PhD student at the University of Alaska-Fairbanks.  He has a passion for space weather and shares his expertise through educational content and sharing his incredible aurora photography.  Vincent Ledvina, welcome to the show. Our email officer Jen is continuing to handle the incoming messages from our listeners. Reach us here: email@weatherbrains.com. Diving into WeatherNation (16:30) ChaserCon (19:00) Looking back at El Reno tornado (24:30) Dealing with problematic storm chasers on social media (41:30) Aurora science and key misconceptions (01:03:15) Factors contributing to 2024 auroras (01:06:00) How to start chasing auroras and tools needed (01:12:00) Upcoming equinox and improving aurora prospects (01:34:00) Polar stratospheric clouds (01:38:10) Farthest south for observable auroras (01:40:15) The Astronomy Outlook with Tony Rice (No segment this week) This Week in Tornado History With Jen (58:02) National Weather Round-Up (01:48:00) E-Mail Segment (01:45:00) and more! Web Sites from Episode 937: NSCS 2024 Erik Fox on X Vincent Ledvina: The Aurora Guy on Facebook NOAA/NWS Space Weather Prediction Center Dr. Tamitha Skov on X The Aurora Guy Picks of the Week: Vincent Ledvina - X5 Solar Flare Erupts from Sun James Aydelott - Goodyear Blimp on X: Rose Bowl sunset view Jen Narramore - Foghorn Wiki article Rick Smith - Probabilistic Hazards Outlook Neil Jacobs - Out Troy Kimmel - Out Kim Klockow-McClain - Out Bill Murray - Foghorn James Spann - Chris Vagasky on X: Snow Tornadoes The WeatherBrains crew includes your host, James Spann, plus other notable geeks like Troy Kimmel, Bill Murray, Rick Smith, James Aydelott, Jen Narramore, Dr. Neil Jacobs, and Dr. Kim Klockow-McClain. They bring together a wealth of weather knowledge and experience for another fascinating podcast about weather.

Tief in den Dschungeln der Welten leben Grunge (oder doch Grungs oder Grünge - egal), kleine froschähnliche Humanoide mit einem potenten Gift. In dieser Folge stellen wir euch diese spannenden Kreaturen und ihre Lebensweise vor. Großer Shoutout nochmal an Karl, der sich diese Folge gewünscht hat. Sollte euch die Folge zu lang sein oder ihr euch nur für einzelne Teile interessieren, findet ihr hier die Kapitelmarker für die gesamte Folge: (01:59) Trims Geschichte (05:03) Grunge außerhalb von D&D (09:16) Grunge in D&D (12:02) Physische Merkmale von Grungen (14:59) Lebensweise und Gesellschaft der Grunge (23:13) Die Kasten der Grunge (39:22) Spielwerte von Grungen (45:23) Kampftaktiken von Okiti (58:31) Grung-NSC: Quarl Download der NSC-Tabelle und von Quarls Spielwerten: ⁠dndbpod.webador.de/dokumente⁠ Portraits und Spielwerte der NSCs aus unseren anderen Folgen findet ihr auch auf unserem ⁠Instagram⁠-Account. Wenn ihr uns unterstützen wollt, könnt ihr das auf ⁠PayPal⁠ oder ⁠BuyMeACoffee⁠ machen!

„Down We Go“ heißt es diese Woche, wenn Matthias Patrick in der Mittagspause auf einen Ausflug in das gleichnamige kleine aber feine Rollenspiel entführt. Minimalisitische Regeln treffen hier auf eine Instant-Charaktererschaffung in einem System, das voll auf Schnelligkeit setzt. Dafür erwartet die Spielleitung ein innovativeres und umfangreiches Framework, mit dem sich abwechslungsreiche und frische „Dungeons“ in verschiedensten Formen bauen lassen. Gerade dieser Part macht Down We Go zu einer wertvollen systemübergreifenden. Ressource für das Schreiben von Fantasyabenteuern. Besonders spannend: Mechaniken wie „Lore as Loot“, die immer wieder alle Spielenden anregen, ihre eigenen Ideen ins Spiel zu werfen. Dann ist da noch das Setting mit der sich ständig wandelnden Stadt Infinopolis, die abseits des Dungeons mit vielen Orten, weirden Fraktionen und noch weirderen NSCs ganz eigene Abenteuer bietet. Zum Schluss bietet Down We Go noch Soloregeln. Viel Spaß beim Hören!

Dr. Rajeswari (Raji) Pillai Rajagopalan is the Director of the Centre for Security, Strategy & Technology (CSST) at the Observer Research Foundation, New Delhi. She is also a Senior Fellow at the Australian Strategic Policy Institute (ASPI) in Canberra. In 2020, she was Co-chair for a thematic group on “Strategic Technologies” for Science, Technology, and Innovation Policy (STIP 2020) work, attached to the Office of Principal Scientific Advisor, Government of India and Department of Science and Technology, Government of India. Dr. Rajagopalan was the Technical Advisor to the United Nations Group of Governmental Experts (GGE) on Prevention of Arms Race in Outer Space (PAROS) (July 2018-July 2019). She was also a Non-Resident Indo-Pacific Fellow at the Perth USAsia Centre from April-December 2020. As a senior Asia defense writer for The Diplomat, she writes a weekly column on Asian strategic issues. Dr. Rajagopalan joined ORF after a five-year stint at the National Security Council Secretariat (2003-2007), Government of India, where she was an Assistant Director. Prior to joining the NSCS, she was Research Officer at the Institute of Defence Studies and Analyses, New Delhi. She was also a Visiting Professor at the Graduate Institute of International Politics, National Chung Hsing University, Taiwan in 2012.Dr. Rajagopalan has authored/ co-authored or edited more than ten books including ORF-Global Policy Journal Special Issue, Future Warfare and Technology: Issues and Strategies (2022), Military Ambitions and Competition in Space: The Role of Alliances (2021), Global Nuclear Security: Moving Beyond the NSS (2018), Space Policy 2.0 (2017), Nuclear Security in India (2015), Clashing Titans: Military Strategy and Insecurity among Asian Great Powers (2012), The Dragon's Fire: Chinese Military Strategy and Its Implications for Asia (2009). She has published research essays in edited volumes, and in peer reviewed journals such as India Review, Strategic Studies Quarterly, Air and Space Power Journal, International Journal of Nuclear Law and Strategic Analysis. She has also contributed essays to newspapers such as The Washington Post, The Wall Street Journal, Times of India, and The Economic Times. She has been invited to speak at international fora including the United Nations Disarmament Forum (New York), the UN Committee on the Peaceful Uses of Outer Space (COPUOS) (Vienna), Conference on Disarmament (Geneva), ASEAN Regional Forum (ARF) and the European Union.EPISODE NOTES:Follow NucleCast on Twitter at @NucleCastEmail comments and story suggestions to NucleCast@anwadeter.orgSubscribe to NucleCast podcastRate the show

Ihr wollt einen Nothic in eurer nächsten Sitzung einsetzen? Dann ist dieser Teil genau das Richtige für euch - heute sprechen wir über die Spielwerte und Kampftaktiken dieser verfluchten Magier und würfeln einen lustigen NSC aus. Sollte euch die Folge zu lang sein oder ihr euch nur für einzelne Teile interessieren, findet ihr hier die Kapitelmarker für die gesamte Folge: (01:09) Rekapitulation: Was sind Nothics? (03:47) Eine Betrachtung der Spielwerte von Rankengebietern und Rankenmarionetten (14:57) Kampftaktiken für Rankengebieter (23:58) NSC Download der NSC-Tabelle und von Spielwerten: dndbpod.webador.de/dokumente Portraits und Spielwerte der NSCs aus unseren anderen Folgen findet ihr auch auf unserem Instagram-Account. Wenn ihr uns unterstützen wollt, könnt ihr das auf PayPal oder BuyMeACoffee machen!

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2023.08.04.551937v1?rss=1 Authors: Luciani, M., Garsia, C., Beretta, S., Petiti, L., Peano, C., Merelli, I., Cifola, I., Miccio, A., Meneghini, V., Gritti, A. Abstract: Human induced pluripotent stem cell-derived neural stem/progenitor cells (hiPSC-NSCs) are a promising source for cell therapy approaches to treat neurodegenerative and demyelinating disorders. Despite ongoing efforts to characterize hiPSC-derived cells in vitro and in vivo, we lack comprehensive genome- and transcriptome-wide studies addressing hiPSC-NSC identity and safety, which are critical for establishing accepted criteria for prospective clinical applications. Here, we evaluated the transcriptional and epigenetic signatures of hiPSCs and differentiated hiPSC-NSC progeny, finding that the hiPSC-to-NSC transition results in a complete loss of pluripotency and the acquisition of a radial glia-associated transcriptional signature. Importantly, hiPSC-NSCs share with somatic human fetal NSCs (hfNSCs) the main transcriptional and epigenetic patterns associated with NSC-specific biology. In vivo, long-term observation (up to 10 months) of mice intracerebrally transplanted as neonates with hiPSC-NSCs showed robust engraftment and widespread distribution of human cells in the host brain parenchyma. Engrafted hiPSC-NSCs displayed multilineage potential and preferentially generated glial cells. No hyperproliferation, tumor formation, or expression of pluripotency markers was observed. Finally, we identified a novel role of the Sterol Regulatory Element Binding Transcription Factor 1 (SREBF1) in the regulation of astroglial commitment of hiPSC-NSCs. Overall, these comprehensive in vitro and in vivo analyses provide transcriptional and epigenetic reference datasets to define the maturation stage of NSCs derived from different hiPSC sources, and to clarify the safety profile of hiPSC-NSCs, supporting their continuing development as an alternative to somatic hfNSCs in treating neurodegenerative and demyelinating disorders. Copy rights belong to original authors. Visit the link for more info Podcast created by Paper Player, LLC

Was entsteht, wenn Magiern arkane Experimente missglücken? Ein Nothic. Erfahrt in dieser Folge alles über diese entstellten Monster und ihre geheimnisvollen Fähigkeiten. Sollte euch die Folge zu lang sein oder ihr euch nur für einzelne Teile interessieren, findet ihr hier die Kapitelmarker für die gesamte Folge: (01:11) Trims Geschichte (05:05) Nothics und vergleichbare Sagen aus der echten Welt (07:14) Nothics in D&D (09:25) Physische Merkmale von Nothics (11:17) Entstehung und Lebensweise von Nothics (33:17) Abschluss Portraits und Spielwerte der NSCs aus unseren anderen Folgen findet ihr auch auf unserem ⁠Instagram⁠-Account. Wenn ihr uns unterstützen wollt, könnt ihr das auf ⁠PayPal⁠ oder ⁠BuyMeACoffee⁠ machen!

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2023.07.02.547346v1?rss=1 Authors: Hong, Y., Zhang, Z., Yangzom, T., Chen, A., Lundberg, B. C., Fang, E. F., Sullivan, G. J., Tzoulis, C., Bindoff, L. A., Liang, K. X. Abstract: Alpers' syndrome is an early-onset neurodegenerative disorder usually caused by biallelic pathogenic variants in the gene encoding the catalytic subunit of polymerase-gamma (POLG), which is essential for mitochondrial DNA (mtDNA) replication. The disease is progressive, incurable, and inevitably it leads to death from drug-resistant status epilepticus. The neurological features of Alpers' syndrome are intractable epilepsy and developmental regression, with no effective treatment; the underlying mechanisms are still elusive, partially due to lack of good experimental models. Here, we generated the patient-derived induced pluripotent stem cells from one Alpers' patient carrying the compound heterozygous mutations of A467T (c.1399G greater than A) and P589L (c.1766C greater than T), and further differentiated them into cortical organoids and neural stem cells (NSCs) for mechanistic studies of neural dysfunction in Alpers' syndrome. Patient cortical organoids exhibited a phenotype that faithfully replicated the molecular changes found in patient postmortem brain tissue, as evidenced by cortical neuronal loss and depletion of mtDNA and complex I (CI). Patient NSCs showed mitochondrial dysfunction leading to ROS overproduction and downregulation of the NADH pathway. More importantly, the NAD+ precursor nicotinamide riboside (NR) significantly ameliorated mitochondrial defects in patient brain organoids. Our findings demonstrate that the iPSC model and brain organoids are good in vitro models of Alpers' disease; this first-in-its-kind stem cell platform for Alpers' syndrome enables therapeutic exploration and has identified NR as a viable drug candidate for Alpers' disease and, potentially, other mitochondrial diseases with similar causes. Copy rights belong to original authors. Visit the link for more info Podcast created by Paper Player, LLC

In this episode your hosts, Tony D'Angelo & Alix Cohler continue their discussion on Core Genius and the Commitment Cultivator.

In this episode your hosts, Tony D'Angelo & Alix Cohler discuss the NSCS Session on Core Genius and Empowerment Focuser.

In this episode your hosts, Tony D'Angelo & Alix Cohler discuss the  NSCS Session on Real GPA.

Weiter geht's mit Rankengebietern - in diesem Teil sprechen wir über die Spielwerte und Kampftaktiken von den mysteriösen Hütern der Wälder und würfeln unseren unheimlichen NSC aus. Sollte euch die Folge zu lang sein oder ihr euch nur für einzelne Teile interessieren, findet ihr hier die Kapitelmarker für die gesamte Folge: (01:15) Rekapitulation: Was sind Rankengebieter? (04:22) Eine Betrachtung der Spielwerte von Rankengebietern und Rankenmarionetten (20:26) Kampftaktiken für Rankengebieter (38:20) NSC Download der NSC-Tabelle und von Spielwerten: dndbpod.webador.de/dokumente Portraits und Spielwerte der NSCs aus unseren anderen Folgen findet ihr auch auf unserem Instagram-Account. Wenn ihr uns unterstützen wollt, könnt ihr das auf PayPal oder BuyMeACoffee machen!

In der heutigen Folge sprechen wir über Rankengebieter, die geheimnisvollen Hüter und Beschützer der Wälder. Erfahrt in dieser Folge, wie Rankengebieter entstehen, wie sie andere Pflanzenwesen kontrollieren und über Nacht ihre Wälder vergrößern. Sollte euch die Folge zu lang sein oder ihr euch nur für einzelne Teile interessieren, findet ihr hier die Kapitelmarker für die gesamte Folge: (01:06) Trims Geschichte (04:03) Rankengebieterähnliche Phänomene aus Mythologie und der echten Welt (14:20) Rankengebieter in D&D (16:23) Physische Merkmale von Rankengebietern (23:00) Lebensweise von Rankengebietern (43:06) Der Lebenszyklus von Rankenmarionetten und Rankengebietern (54:46) Abschluss Vielen Dank an Kobold Press für die Erlaubnis diese spannende Folge machen zu dürfen. Schaut euch Tome of Beasts und andere tolle Bücher auf der Webseite von Kobold Press an. Tome of Beasts wurde von Ulisses auf Deutsch übersetzt und ist hier erhältlich. Es handelt sich um eine ehrliche Empfehlung und nicht um bezahlte Werbung.  Portraits und Spielwerte der NSCs aus unseren anderen Folgen findet ihr auch auf unserem Instagram-Account. Wenn ihr uns unterstützen wollt, könnt ihr das auf PayPal oder BuyMeACoffee machen!

Weiter geht's mit Grünen Drachen - in diesem Teil sprechen wir über die Spielwerte und Kampftaktiken von diesen Meisterlügnern und würfeln Umerlothtoreir aus - den NSC dieser Folge. Sollte euch die Folge zu lang sein oder ihr euch nur für einzelne Teile interessieren, findet ihr hier die Kapitelmarker für die gesamte Folge: (01:30) Rekapitulation: Was sind Grüne Drachen? (02:55) Eine (kritische) Betrachtung der Spielwerte von D&D (34:46) Kampftaktiken für Grüne Drachen (49:01) NSC: Umerlothtoreir Download der NSC-Tabelle und von Umerlothtoreirs Spielwerten: dndbpod.webador.de/dokumente Portraits und Spielwerte der NSCs aus unseren anderen Folgen findet ihr auch auf unserem Instagram-Account. Wenn ihr uns unterstützen wollt, könnt ihr das auf PayPal oder BuyMeACoffee machen!

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2023.04.17.537129v1?rss=1 Authors: Hardt, R., Dehghani, A., Schoor, C., Goedderz, M., Cengiz Winter, N., Ahmadi, S., Sharma, R., Schork, K., Eisenacher, M., Gieselmann, V., Winter, D. Abstract: Oligodendrocytes are generated via a two-step mechanism from pluripotent neural stem cells (NSCs): after differentiation of NSCs to oligodendrocyte precursor/NG2 cells (OPCs), they further develop into mature oligodendrocytes. The first step of this differentiation process is only incompletely understood. In this study, we utilized the neurosphere assay to investigate NSC to OPC differentiation in a time course-dependent manner by mass spectrometry-based (phospho-) proteomics. We identify double cortin like kinase 1 (Dclk1) as one of the most prominently regulated proteins in both datasets, and show that it undergoes a gradual transition between its short/long isoform during NSC to OPC differentiation. This is regulated by phosphorylation of its SP-rich region, resulting in inhibition of proteolytic Dclk1 long cleavage, and therefore Dclk1 short generation. Through interactome analyses of different Dclk1 isoforms by proximity biotinylation, we characterize their individual putative interaction partners and substrates. Copy rights belong to original authors. Visit the link for more info Podcast created by Paper Player, LLC

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2023.04.13.536811v1?rss=1 Authors: Junaid, M., Choe, H. K., Kondoh, K., Lee, E. J., Lim, S. B. Abstract: The neural stem cells (NSCs) in the hypothalamus are relatively more narrowly defined than in other neurogenic regions of the postnatal brain. By leveraging single-cell RNA sequencing (scRNA-seq) data, we generated an integrated reference dataset comprising 296,282 cells from postnatal hypothalamic regions and the adjacent region, bed nucleus of the stria terminalis (BNST), and identified 30 hypothalamic neuronal and non-neuronal cell populations. The analyses of their gene expression pattern, and specific differentiation trajectories reveal the presence of NSCs and intermediate progenitor cells (IPCs) that show a continuum of activation and differentiation processes in the hypothalamus after birth. Through comparative analyses of the integrated dataset with our lab-generated Connect-seq data obtained from the whole hypothalamus, we further assessed the technical validity of the dataset presented in this study. Our large-scale unified scRNA-seq dataset with harmonized cell-level metadata can serve as a valuable resource for investigating cell type-specific gene expression and cellular differentiation trajectories in the postnatal mouse hypothalamus. Copy rights belong to original authors. Visit the link for more info Podcast created by Paper Player, LLC

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2023.04.13.536749v1?rss=1 Authors: Rodriguez-Perez, L. M., Ojeda-Perez, B., Garcia-Bonilla, M., Lopez-de-San-Sebastian, J., Gonzalez-Garcia, M., Fernandez-Munoz, B., Sanchez-Pernaute, R., Garcia-Martin, M. L., Dominguez-Pino, D., Cardenas-Garcia, C., Jimenez-Lara, A. J., Paez-Gonzalez, P. Abstract: Germinal matrix hemorrhages (GMH) and the consequent posthemorrhagic hydrocephalus (PHH) are among the most common and severe neurological complications of preterm birth that require lifelong complex neurosurgical care. GMH and PHH provoke disruption of neuroepithelium/ependyma development, a key structure implicated in brain development and homeostasis. Neuroepithelial/ependymal damage causes lifelong cognitive and motor deficits; however, no therapy is directed to recover the damaged ependyma. This study is aimed to test the possibilities of ependymal repair in GMH/PHH using neural stem cells (NSCs) or ependymal progenitors (EpPs). Thus, it sets the basis for a therapeutic approach to treating ependymal damage and preventing brain developmental deficits. GMH/PHH was induced in 4-day-old mice using different experimental procedures involving collagenase, blood, or blood serum injections. PHH severity was characterized using magnetic resonance, immunofluorescence, and protein expression quantification with mass spectrometry. Additionally, a new exvivo approach using ventricular walls from mice developing moderate and severe GMH/PHH was generated to study ependymal restoration and wall regeneration after stem cell treatments. NSCs or EpPs obtained from newborn mice were transplanted in the explants, and pretreatment with mesenchymal stem cells (MSCs) was tested. Ependymal differentiation and the effect of MSC-conditioned microenvironment were investigated in both explants and primary cultures. In the animals, PHH severity was correlated with the extension of GMH, ependymal disruption, astroglial/microglial reactions, and ventriculomegaly. In the explants, the severity and extension of GMH hindered the survival rates of the transplanted NSCs/EpPs. In the explants affected with GMH, new multiciliated ependymal cells could be generated from transplanted NSCs and, more efficiently, from EpPs. Blood and TNF negatively affected ciliogenesis in cells expressing Foxj1. Pretreatment with mesenchymal stem cells (MSC) improved the survival rates of EpPs and ependymal differentiation while reducing the edematous and inflammatory conditions in the explants. In conclusion, in GMH/PHH, the ependyma can be restored from either NSC or EpP transplantation, being EpPs in an MSC-conditioned microenvironment more efficient for this purpose. Modifying the neuroinflammatory microenvironment by MSC pretreatment positively influenced the success of the ependymal restoration. Copy rights belong to original authors. Visit the link for more info Podcast created by Paper Player, LLC

Dungeons & Dragons - Drachen stecken im Name des Spiels. In dieser Folge sprechen wir über die Lebensweise von Grünen Drachen, die verschlagenen Meister der Lüge und Intrige. In Teil 2 werden wir uns dann mit Spielwerten und Kampftaktiken beschäftigen und unseren NSC auswürfeln. Sollte euch die Folge zu lang sein oder ihr euch nur für einzelne Teile interessieren, findet ihr hier die Kapitelmarker für die gesamte Folge: (01:06) Trims Geschichte (04:39) Grüne Drachen außerhalb von D&D (09:39) Grüne Drachen in D&D (13:03) Physische Merkmale von Grünen Drachen (25:40) Lebensweise von Grünen Drachen (46:51) Lebensraum und Horte Grüner Drachen (56:33) Abschluss Portraits und Spielwerte der NSCs aus unseren anderen Folgen findet ihr auf unserem Instagram-Account oder unserer Homepage. Wenn ihr uns unterstützen wollt, könnt ihr das auf PayPal oder BuyMeACoffee machen!

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2023.03.14.532547v1?rss=1 Authors: Fuchigami, T., Itokazu, Y., Yu, R. K. Abstract: The postnatal neural stem cell (NSC) pool hosts quiescent and activated radial glia-like NSCs contributing to neurogenesis throughout adulthood. However, the underlying regulatory mechanism during the transition from quiescent NSCs to activated NSCs in the postnatal NSC niche is not fully understood. Lipid metabolism and lipid composition play important roles in regulating NSC fate determination. Biological lipid membranes define the individual cellular shape and help maintain cellular organization and are highly heterogenous in structure and there exist diverse microdomains (also known as lipid rafts), which are enriched with sugar molecules, such as glycosphingolipids. An often overlooked but key aspect is that the functional activities of proteins and genes are highly dependent upon their molecular environments. We previously reported that ganglioside GD3 is the predominant species in NSCs and that the reduced postnatal NSC pools are observed in global GD3-synthase knockout (GD3S-KO) mouse brains. The specific roles of GD3 in determining the stage and cell-lineage determination of NSCs remain unclear, since global GD3S-KO mice cannot distinguish if GD3 regulates postnatal neurogenesis or developmental impacts. Here we show that inducible GD3 deletion in postnatal radial glia-like NSCs promotes the NSC activation, resulting in the loss of the long-term maintenance of the adult NSC pools. The reduced neurogenesis in the subventricular zone (SVZ) and the dentate gyrus (DG) of GD3S-conditional-knockout mice led to impaired olfactory and memory functions. Thus, our results provide convincing evidence that postnatal GD3 maintains the quiescent state of radial glia-like NSCs in the adult NSC niche. Copy rights belong to original authors. Visit the link for more info Podcast created by Paper Player, LLC

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2023.03.04.531101v1?rss=1 Authors: Bielefeld, P., Martirosyan, A., Apresyan, A., Meerhoff, G., Pestana, F., Poovathingal, S., Reijner, N., Koning, W., Clement, R., van de Veen, I., Toledo, E., Dura, I., Hovhannisyan, S., Nilges, B., Bogdoll, A., Kashikar, N., Lucassen, P. J., Belgard, G., Encinas, J. M., Holt, M., Fitzsimons, C. P. Abstract: Moderate Traumatic brain injury (TBI) can result in long-lasting changes in brain function. Although frequently spared from the acute primary injury, the hippocampus becomes affected during a secondary phase that takes place hours, or even days, after TBI, contributing to cognitive deficits. The hippocampus is one of the few brain areas in the adult brain harboring native neural stem cells (NSCs) that continue to generate new neurons (neurogenesis), and to a lesser extent new astrocytes (astrogliogenesis). While deregulation of hippocampal NSCs and neurogenesis have been observed after TBI, very little is known about how TBI may affect hippocampal astrogliogenesis. Here, we aimed to assess how TBI affects hippocampal NSCs and their subsequent commitment to the neuronal or astroglial lineages. Using a controlled cortical impact model of TBI, single cell RNA sequencing and spatial transcriptomics, we observed a cell population-specific increase in NSC-derived neuronal cells and a decrease in NSC-derived astrocytic cells. These cellular changes were associated with cell-population specific changes in gene expression and dysplasia within the dentate gyrus. Overall, our findings support the conclusion that TBI modifies adult hippocampal NSC fate to promote neurogenesis at the cost of astrogliogenesis, and highlights specific cell populations as possible targets to counteract the changes induced by TBI in the hippocampus. Copy rights belong to original authors. Visit the link for more info Podcast created by Paper Player, LLC

Rattenmenschen, Bilgerratten - Okiti haben viele Namen. In unserer vierten Folge stellen wir euch die spannenden Hintergründe zu Okiti vor; ein Volk, das den meisten von euch noch neu sein dürfte. Sollte euch die Folge zu lang sein oder ihr euch nur für einzelne Teile interessieren, findet ihr hier die Kapitelmarker für die gesamte Folge: (01:04) Trims Geschichte (04:29) Okiti außerhalb von D&D (07:35) Okiti in D&D (17:00) Physische Merkmale von Okiti (23:35) Lebensweise und Gesellschaft der Okiti (41:12) Wo findet man Okiti? (47:54) Gifte und Krankheiten (52:51) Spielwerte von Okiti (59:00) Kampftaktiken von Okiti (1:07:05) Okiti-NSC: Kikikatislaminita Vielen Dank an NordGames LLC für die Erlaubnis diese spannende Folge machen zu dürfen. Schaut euch Ultimate Bestiary: Revenge of the Horde und andere tolle Bücher auf der Webseite an: https://nordgamesllc.com/. Es handelt sich um eine ehrliche Empfehlung und nicht um bezahlte Werbung. Download der NSC-Tabelle und von Kikis Spielwerten: dndbpod.webador.de/dokumente Portraits und Spielwerte der NSCs aus unseren anderen Folgen findet ihr auch auf unserem Instagram-Account. Wenn ihr uns unterstützen wollt, könnt ihr das auf PayPal oder BuyMeACoffee machen!

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2023.02.14.528567v1?rss=1 Authors: Li, J., Godoy, M. I., Zhang, A. J., Diamante, G., Ahn, I. S., Cebrian-Silla, A., Alvarez-Buylla, A., Yang, X., Novitch, B., Zhang, Y. Abstract: Embryonic neural stem cells (NSCs, i.e., radial glia) in the ventricular-subventricular zone (V-SVZ) generate the majority of neurons and glia in the forebrain. Postnatally, embryonic radial glia disappear and a subpopulation of radial glia transition into adult NSCs. As this transition occurs, widespread neurogenesis in brain regions such as the cerebral cortex ends. The mechanisms that regulate the postnatal disappearance of radial glia and the ending of embryonic neurogenesis remain poorly understood. Here, we show that PR domain-containing 16 (Prdm16) promotes the disappearance of radial glia and the ending of neurogenesis in the cerebral cortex. Genetic deletion of Prdm16 from NSCs leads to the persistence of radial glia in the adult V-SVZ and prolonged postnatal cortical neurogenesis. Mechanistically, Prdm16 induces the postnatal reduction in Vascular Cell Adhesion Molecule 1 (Vcam1). The postnatal disappearance of radial glia and the ending of cortical neurogenesis occur normally in Prdm16-Vcam1 double conditional knockout mice. These observations reveal novel molecular regulators of the postnatal disappearance of radial glia and the ending of embryonic neurogenesis, filling a key knowledge gap in NSC biology. Copy rights belong to original authors. Visit the link for more info Podcast created by Paper Player, LLC

In dieser Bonusfolge schließen wir mit den Goblinoiden ab und stellen euch Maglubiyet und den Aufbau und die Struktur einer Goblinoidenheerschar vor. Sollte euch die Folge zu lang sein oder ihr euch nur für einzelne Teile interessieren, findet ihr hier die Kapitelmarker für die gesamte Folge: (01:39) Maglubiyet (13:45) Die Ebene Acheron (21:41) Die Goblinoidenheerschar (32:45) Goblins in der Heerschar (38:17) Grottenschrate in der Heerschar (44:41) Hobgoblins in der Heerschar (56:58) Kampftaktik einer Heerschar (1:02:01) Goblinoidenlager (1:09:51) Lager auf Zufallstabellen auswürfeln Download der Lager-Tabelle: dndbpod.webador.de/dokumente Portraits und Spielwerte der NSCs aus unseren anderen Folgen findet ihr auch auf unserem Instagram-Account. Wenn ihr uns unterstützen wollt, könnt ihr das auf PayPal oder BuyMeACoffee machen!

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2023.01.28.526021v1?rss=1 Authors: Hong, Y., Kristiansen, C. K., Chen, A., NIDO, G. S., Hoyland, L. E., Ziegler, M., Sullivane, G. J., Bindoff, L. A., Liang, K. X. Abstract: Diseases caused by POLG mutations are the most common form of mitochondrial disease and associated with phenotypes of varying severity. Clinical studies have shown that patients with compound heterozygous POLG mutations have a lower survival rate than patients with homozygous mutations, but the molecular mechanisms behind this remain unexplored. Using an induced pluripotent stem cell (iPSC) model, we investigate differences between homozygous and compound heterozygous genotypes in different cell types, including patient-specific fibroblasts, iPSCs, and iPSC-derived neural stem cells (NSCs) and astrocytes. We found that compound heterozygous lines exhibited greater impairment of mitochondrial function in NSCs than homozygous NSCs, but not in fibroblasts, iPSCs, or astrocytes. Compared with homozygous NSCs, compound heterozygous NSCs exhibited more severe functional defects, including reduced ATP production, loss of mitochondrial DNA (mtDNA) copy number and complex I expression, disturbance of NAD+ metabolism, and higher ROS levels, which further led to cellular senescence and activation of mitophagy. RNA sequencing analysis revealed greater downregulation of mitochondrial and metabolic pathways, including the citric acid cycle and oxidative phosphorylation, in compound heterozygous NSCs. Our iPSC-based disease model can be widely used to understand the genotype-phenotype relationship of affected brain cells in mitochondrial diseases, and further drug discovery applications. Copy rights belong to original authors. Visit the link for more info Podcast created by Paper Player, LLC

Wir widmen uns in dieser Folge dem letzten goblioniden Volk - Grottenschraten - in Dungeons & Dragons und sprechen über alles, was diese spannenden Kreaturen ausmacht. Sollte euch die Folge zu lang sein oder ihr euch nur für einzelne Teile interessieren, findet ihr hier die Kapitelmarker für die gesamte Folge: (01:25) Trims Kurzgeschichte (03:59) Grottenschrate außerhalb von D&D (07:10) Grottenschrate in D&D (09:12) Physische Charakteristika von Grottenschrate (18:44) Der Grottenschrat-Pantheon (Grankhul, Hruggek, Skiggaret und Maglubiyet) (39:01) Allgemeine Lebensweise von Grottenschraten (44:58) Soziologie von Grottenschraten (41:08) Wo und wie leben Grottenschrate? (50:59) Spielwerte von Grottenschraten (57:56) Kampftaktiken für Grottenschrate (1:08:07) Grottenschrat-NSC: Klarg der Große Download der NSC-Tabelle und Spielwerte von Klarg dem Großen: dndbpod.webador.de/dokumente. Portraits und Spielwerte der NSCs findet ihr auch auf unserem Instagram-Account. Wenn ihr uns unterstützen wollt, könnt ihr das auf PayPal oder BuyMeACoffee machen! Wichtigste Quellen: Volos Almanach der Monster (insb. S. 41-42) Monster Manual (insb. S. 152) The Monsters Know What They're Doing (S. 23)

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2023.01.10.523252v1?rss=1 Authors: del Puerto, A., Marti-Prado, B., Barrios-Munoz, A. L., Lopez-Fonseca, C., Pose-Utrilla, J., Alcover-Sanchez, B., Cesca, F., Schiavo, G., Campanero, M. R., Farinas, I., Iglesias, T., Porlan, E. Abstract: In the adult mammalian brain, neural stem cells (NSCs) located in highly restricted niches sustain the generation of new neurons that integrate into existing circuits. A reduction in adult neurogenesis is linked to ageing and neurodegeneration, whereas dysregulation of proliferation and survival of NSCs have been hypothesized to be at the origin of glioma. Thus, unravelling the molecular underpinnings of the regulated activation that NSCs must undergo to proliferate and generate new progeny is of considerable relevance. current research has identified cues promoting or restraining NSCs activation. Yet, whether NSCs depend on external signals to survive or if intrinsic factors establish a threshold for sustaining their viability remains elusive, even if this knowledge could involve potential for devising novel therapeutic strategies. Kidins220 (Kinase D-interacting substrate of 220 kDa) is an essential effector of crucial pathways for neuronal survival and differentiation. It is dramatically altered in cancer and in neurological and neurodegenerative disorders, emerging as a regulatory molecule with important functions in human disease. Herein, we discover severe neurogenic deficits and hippocampal-based spatial memory defects in Kidins220 deficient mice. Mechanistically, we demonstrate that Kidins220-dependent activation of AKT in response to EGF restraints GSK3 activity preventing NSCs apoptosis. Hence, Kidins220 levels set a molecular threshold for survival in response to mitogens, allowing adult NSCs to proliferate. Our study identifies Kidins220 as a key player for sensing the availability of growth factors to sustain adult neurogenesis, uncovering a molecular link that may help paving the way towards neurorepair. Copy rights belong to original authors. Visit the link for more info Podcast created by Paper Player, LLC

In unserer zweiten Folge geht's um Hobgoblins in Dungeons & Dragons und alles, was es über sie zu wissen gibt: von ihrer Lebensweise und Soziologie bis hin zu Kampftaktiken für Spielleiter:innen. Sollte euch die Folge zu lang sein oder ihr euch nur für einzelne Teile interessieren, findet ihr hier die Kapitelmarker für die gesamte Folge: (01:30) Trims Kurzgeschichte (03:41) Hobgoblins außerhalb von D&D (05:52) Hobgoblins in D&D (08:13) Physische Charakteristika von Hobgoblins (15:57) Der Hobgoblin-Pantheon (Maglubiyet, Nomog-Geaya und Bargrivyek) (22:45) Eisenschatten (27:03) Lebensweise und Soziologie von Hobgoblins (41:08) Wo und wie leben Hobgoblins? (50:30) Spielwerte von Hobgoblins (55:42) Kampftaktiken für Hobgoblins (1:10:40) Hobgoblin-NSC: Norat, die Ehrlose Download der NSC-Tabelle und Spielwerte von Norat, der Ehrlosen: dndbpod.webador.de/dokumente Portraits und Spielwerte der NSCs findet ihr auch auf unserem Instagram-Account. Wenn ihr uns unterstützen wollt, könnt ihr das auf PayPal oder BuyMeACoffee machen! Wichtigste Quellen: Volos Almanach der Monster (insb. S. 42-44) Monster Manual (insb. S. 158-159) The Monsters Know What They're Doing (S. 19-21)

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2022.12.26.521937v1?rss=1 Authors: Mancini, L., Guirao, B., Ortica, S., Labusch, M., Cheysson, F., Bonnet, V., Phan, M. S., Herbert, S., Mahou, P., Menant, E., Bedu, S., Tinevez, J.-Y., Baroud, C., Beaurepaire, E., Bellaiche, Y., Bally-Cuif, L., Dray, N. Abstract: The maintenance of neural stem cells (NSCs) in the adult brain depends on their activation frequency and division mode. We use long-term intravital imaging of NSCs in the zebrafish adult telencephalon to link activation and division mode with predictive cellular and molecular parameters. We reveal that apical surface area and expression of the Notch ligand DeltaA predict NSC activation frequency, while deltaA expression marks NSC commitment to neurogenesis. We also find that deltaA-negative NSCs constitute the bona fide self-renewing NSC pool and systematically engage in asymmetric divisions generating a self-renewing deltaAneg and a neurogenic deltaApos NSC. Finally, modulation of Notch signaling during imaging indicates that the prediction of activation frequency by apical size, and the asymmetric divisions of deltaAneg NSCs, are functionally independent of Notch. These results provide dynamic qualitative and quantitative readouts of NSC lineage progression in vivo and support a hierarchical organization of NSCs in differently fated sub-populations. Copy rights belong to original authors. Visit the link for more info Podcast created by Paper Player, LLC

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2022.12.14.520430v1?rss=1 Authors: Morrow, C. S., Tweed, K., Arndt, Z. P., Walsh, A. J., Peng, B., Risgaard, R. D., Klosa, P. C., Chi, M. M., Wallace, E. P., Jones, M. V., Roopra, A., Skala, M. C., Moore, D. L. Abstract: Neural stem cells (NSCs) in the adult brain are primarily quiescent but can activate and enter the cell cycle to produce newborn neurons. NSC quiescence can be regulated by disease, injury, and age, however our understanding of NSC quiescence is limited by technical limitations imposed by the bias of markers used to isolate each population of NSCs and the lack of live-cell labeling strategies. Fluorescence lifetime imaging (FLIM) of autofluorescent metabolic cofactors has previously been used in other cell types to study shifts in cell states driven by metabolic remodeling that change the optical properties of these endogenous fluorophores. Here we asked whether autofluorescence could be used to discriminate NSC activation state. We found that quiescent NSCs (qNSCs) and activated NSCs (aNSCs) each have unique autofluorescence intensity and fluorescence lifetime profiles. Additionally, qNSCs specifically display an enrichment of a specific autofluorescent signal localizing to lysosomes that is highly predictive of cell state. These signals can be used as a graded marker of NSC quiescence to predict cell behavior and track the dynamics of quiescence exit at single cell resolution in vitro and in vivo. Through coupling autofluorescence imaging with single-cell RNA sequencing in vitro and in vivo, we provide a high-resolution resource revealing transcriptional features linked to rapid NSC activation and deep quiescence. Taken together, we describe a single-cell resolution, non-destructive, live-cell, label-free strategy for measuring NSC activation state in vitro and in vivo and use this tool to expand our understanding of adult neurogenesis. Copy rights belong to original authors. Visit the link for more info Podcast created by Paper Player, LLC

In unserer ersten Folge erzählen wir - das sind Silvan und Sebastian - euch alles, was ihr zu Goblins in Dungeons & Dragons wissen müsst: von ihrer Lebensweise und Soziologie bis hin zu Kampftaktiken für Spielleiter:innen. Sollte euch die Folge zu lang sein oder ihr euch nur für einzelne Teile interessieren, findet ihr hier die Kapitelmarker für die gesamte Folge: (01:20) Trims Kurzgeschichte (03:48) Goblins außerhalb von D&D (08:08) Goblins in D&D (09:24) Physische Charakteristika von Goblins (19:05) Maglubiyet, der Mächtige und die drei goblinoiden Völker (24:18) Eigenschaften von Goblins (33:44) Wo leben Goblins? (42:33) Goblinsoziologe und die Kasten (1:03:42) Spielwerte von Goblins (1:09:25) Kampftaktiken für Goblins (1:25:16)Goblin-NSC: Gnazzt Müllschlucker Download der NSC-Tabelle und Spielwerte von Gnazzt Müllschlucker: dndbpod.webador.de/dokumente Portraits und Spielwerte der NSCs findet ihr auch auf unserem Instagram-Account. Wenn ihr uns unterstützen wollt, könnt ihr das auf PayPal oder BuyMeACoffee machen! Wichtigste Quellen: Volos Almanach der Monster (insb. S. 37-40) Monster Manual (insb. S. 141-142) The Monsters Know What They're Doing (S. 15-19) Noch ein Hinweis: Gnazzt, unser ausgewürfelter NSC ist nonbinär. Leider haben wir es in der Aufregung unserer ersten Aufnahme nicht geschafft, non-binäre Pronomen für Gnazzt zu verwenden, sondern haben von 'ihm' (im Sinne von der Goblin) gesprochen. Bitte seht uns diesen Fehler nach - wir lernen und werden hoffentlich mit jeder Folge besser!

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2022.12.05.518892v1?rss=1 Authors: Pastor-Alonso, O., Syeda Zahra, A., Kaske, B., Garcia-Moreno, F., Tetzlaff, F., Bockelmann, E., Grunwald, V., Martin-Suarez, S., Riecken, K., Witte, O. W., Encinas, J. M., Urbach, A. Abstract: In the hippocampus, lifelong neurogenesis is maintained by a pool of multipotent adult neural stem cells (aNSCs) residing in the subgranular zone of the dentate gyrus (DG). Yet, the mechanisms guiding the transition of NSCs from developmental to adult remain unclear. By using nestin-reporter mice deficient for D2, a cyclin expressed mainly postnatally, we show that the aNSC pool is established through D2-dependent proliferation during the first two weeks of life. The absence of D2 allows the normal development of the DG until birth but prevents the postnatal formation of radial glia-like aNSCs. Additionally, retroviral fate mapping demonstrates that aNSCs are born on-site from precursors located in the DG shortly after birth. Altogether, our data suggest that aNSCs are a population distinct from developmental NSCs and thus imply that adult hippocampal neurogenesis is not a mere continuation of development. Copy rights belong to original authors. Visit the link for more info Podcast created by Paper Player, LLC

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2022.12.02.518869v1?rss=1 Authors: Baur, K., Carrillo Garcia, C., San, S., von Hahn, M., Strelau, J., Hölzl-Wenig, G., Mandl, C., Ciccolini, F. Abstract: Late in neural development, the expression of growth/differentiation factor (GDF) 15 increases in the germinal epithelium of the murine ganglionic eminence (GE). However, the function of GDF15 in this region is unknown. We here show that ablation of GDF15 leads to an increase in proliferation of apically and subapically dividing progenitors in the GE. This is associated with faster cell cycle progression in both progenitor groups, and an increase in the total number of cycling progenitors. Enhanced proliferation of apically dividing progenitors leads to a permanent significant increase in the number of ependymal and apical neural stem cells (NSCs). Our data also indicate that the extra proliferation of subapically dividing progenitors causes a transient increase in the number of neuronal progenitors, which is compensated by increased apoptosis. Independent of the genotype, activity of endogenous epidermal growth factor (EGFR) signalling is essential for the proliferation of apically and subapically dividing progenitors. However, lack of GDF15 leads to a reduced cell surface expression of EGFR and altered dynamics of MAPK activation in response to EGF stimulation. Application of exogenous GDF15 rescued the effect of the genotype on the expression of EGFR and decreased proliferation in the mutant GE. Taken together, our results indicate that GDF15 modulates proliferation and growth factor responsiveness of apical progenitors in the developing GE, thereby regulating the number of total ependymal and NSCs. Copy rights belong to original authors. Visit the link for more info Podcast created by Paper Player, LLC

Growth of A Massive Black Hole Via Tidal Disruption Accretion by Seungjae Lee et al. on Wednesday 30 November Stars that are tidally disrupted by the massive black hole (MBH) may contribute significantly to the growth of the MBH, especially in dense nuclear star clusters (NSCs). Yet, this tidal disruption accretion (TDA) of stars onto the MBH has largely been overlooked compared to the gas accretion (GA) channel in most numerical experiments until now. In this work, we implement a black hole growth channel via TDA in the high-resolution adaptive mesh refinement code Enzo to investigate its influence on a MBH seed's early evolution. We find that a MBH seed grows rapidly from $10^3,mathrm{M}_odot$ to $gtrsim 10^6,mathrm{M}_odot$ in 200,Myrs in some of the tested simulations. Compared to a MBH seed that grows only via GA, TDA can enhance the MBH's growth rate by up to more than an order of magnitude. However, as predicted, TDA mainly helps the early growth of the MBH (from $10^{3-4},mathrm{M}_odot$ to $lesssim10^{5},mathrm{M}_odot$) while the later evolution is generally dominated by GA. We also observe that the star formation near the MBH is suppressed when TDA is most active, sometimes with a visible cavity in gas (of size $sim$ a few pc) created in the vicinity of the MBH. It is because the MBH may grow expeditiously with both GA and TDA, and the massive MBH could consume its neighboring gas faster than being replenished by gas inflows. Our study demonstrates the need to consider different channels of black hole accretion that may provide clues for the existence of supermassive black holes at high redshifts. arXiv: http://arxiv.org/abs/http://arxiv.org/abs/2211.02376v2

Growth of A Massive Black Hole Via Tidal Disruption Accretion by Seungjae Lee et al. on Tuesday 29 November Stars that are tidally disrupted by the massive black hole (MBH) may contribute significantly to the growth of the MBH, especially in dense nuclear star clusters (NSCs). Yet, this tidal disruption accretion (TDA) of stars onto the MBH has largely been overlooked compared to the gas accretion (GA) channel in most numerical experiments until now. In this work, we implement a black hole growth channel via TDA in the high-resolution adaptive mesh refinement code Enzo to investigate its influence on a MBH seed's early evolution. We find that a MBH seed grows rapidly from $10^3,mathrm{M}_odot$ to $gtrsim 10^6,mathrm{M}_odot$ in 200,Myrs in some of the tested simulations. Compared to a MBH seed that grows only via GA, TDA can enhance the MBH's growth rate by up to more than an order of magnitude. However, as predicted, TDA mainly helps the early growth of the MBH (from $10^{3-4},mathrm{M}_odot$ to $lesssim10^{5},mathrm{M}_odot$) while the later evolution is generally dominated by GA. We also observe that the star formation near the MBH is suppressed when TDA is most active, sometimes with a visible cavity in gas (of size $sim$ a few pc) created in the vicinity of the MBH. It is because the MBH may grow expeditiously with both GA and TDA, and the massive MBH could consume its neighboring gas faster than being replenished by gas inflows. Our study demonstrates the need to consider different channels of black hole accretion that may provide clues for the existence of supermassive black holes at high redshifts. arXiv: http://arxiv.org/abs/http://arxiv.org/abs/2211.02376v2

Growing Black Holes through Successive Mergers in Galactic Nuclei: I Methods and First Results by Dany Atallah et al. on Monday 21 November We present a novel, few-body computational framework designed to shed light on the likelihood of forming intermediate-mass (IM) and supermassive (SM) black holes (BHs) in nuclear star clusters (NSCs) through successive BH mergers, initiated with a single BH seed. Using observationally motivated NSC profiles, we find that the probability of a ${sim}100 , M_odot$ BH to grow beyond ${sim}1000 , M_odot$ through successive mergers ranges from ${sim}0.1%$ in low-density, low-mass clusters to nearly $90%$ in high-mass, high-density clusters. However, in the most massive NSCs, the growth timescale can be very long ($gtrsim 1,$Gyr); vice versa, while growth is least likely in less massive NSCs, it is faster there, requiring as little as ${sim}0.1,$Gyr. The increased gravitational focusing in systems with lower velocity dispersions is the primary contributor to this behavior. We find that there is a simple "7-strikes-and-you're-in" rule governing the growth of BHs: our results suggest that if the seed survives 7 to 10 successive mergers without being ejected (primarily through gravitational wave recoil kicks), the growing BH will most likely remain in the cluster and will then undergo runaway, continuous growth all the way to the formation of an SMBH (under the simplifying assumption adopted here of a fixed background NSC). Furthermore, we find that rapid mergers enforce a dynamically-mediated "mass gap" between about ${50-300 , M_odot}$ in an NSC. arXiv: http://arxiv.org/abs/http://arxiv.org/abs/2211.09670v2

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2022.11.08.515655v1?rss=1 Authors: Zhang, R., Mao, W., Niu, L., Bao, W., Wang, Y., Yang, Z., Zhu, Y., Song, H., Chen, J., Li, G., Cai, M., Yuan, Z., Dong, J., Zhang, M., Xiong, N., Wei, J., Dong, Z. Abstract: Transplantation of neural stem cells (NSCs) has been proved to promote functional rehabilitation of brain lesions including ischemic stroke. However, the therapeutic effects of NSC transplantation is limited by the low survival and differentiation rates of NSCs due to the harsh environment in the brain after ischemic stroke. Here, we employed NSCs derived from human induced pluripotent stem cells (iPSCs) together with exosomes extracted from NSCs to treat cerebral ischemia induced by middle cerebral artery occlusion/reperfusion (MCAO/R) in mice. The results showed that NSC-derived exosomes significantly reduced the inflammatory response, alleviated oxidative stress after NSC transplantation, and facilitated NSCs differentiation in vivo. The combination of NSCs with exosomes ameliorated the injury of brain tissue including cerebral infarct, neuronal death and glial scarring, and promoted the motor function recovery. To explore the underlying mechanisms, we analyzed the miRNA profiles of NSC-derived exosomes and the potential downstream genes. Our study provided the rationale for the clinical application of NSC-derived exosomes as a supportive adjuvant for NSC transplantation after stroke. Copy rights belong to original authors. Visit the link for more info Podcast created by Paper Player, LLC

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2022.10.31.514562v1?rss=1 Authors: Rieskamp, J. D., Rosado-Burgos, I., Christofi, J. E., Ansar, E., Einstein, D., Walters, A. E., Valentini, V., Bruno, J. P., Kirby, E. D. Abstract: Within the adult mammalian dentate gyrus (DG) of the hippocampus, glutamate stimulates neural stem cell (NSC) self-renewing proliferation, providing a link between adult neurogenesis and local circuit activity. Here, we show that glutamate-induced self-renewal of adult DG NSCs requires glutamate transport via excitatory amino acid transporter 1 (EAAT1) to stimulate lipogenesis. Loss of EAAT1 prevented glutamate-induced self-renewing proliferation of NSCs in vitro and in vivo, with little role evident for canonical glutamate receptors. Transcriptomics and further pathway manipulation revealed that glutamate simulation of NSCs relied on EAAT1 transport-stimulated lipogenesis, likely secondary to intracellular Ca2+ release and glutamate metabolism. Our findings demonstrate a critical, direct role for EAAT1 in stimulating NSCs to support neurogenesis in adulthood, thereby providing insights into a non-canonical mechanism by which NSCs sense and respond their niche. Copy rights belong to original authors. Visit the link for more info Podcast created by Paper Player, LLC

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2022.10.18.512724v1?rss=1 Authors: Micali, N., Ma, S., Li, M., Kim, S.-K., Mato-Blanco, X., Sindhu, S., Arellano, J. I., Gao, T., Duque, A., Santpere, G., Sestan, N., Rakic, P. Abstract: Early telencephalic development involves patterning of the distinct regions and fate specification of the neural stem cells (NSCs). These processes, mainly characterized in rodents, remain elusive in primates and thus our understanding of conserved and species-specific features. Here, we profiled 761,529 single-cell transcriptomes from multiple regions of the prenatal macaque telencephalon. We defined the molecular programs of the early organizing centers and their cross-talk with NSCs, finding primate-biased signaling active in the antero-ventral telencephalon. Regional transcriptomic divergences were evident at early states of neocortical NSC progression and in differentiated neurons and astrocytes, more than in intermediate transitions. Finally, we show that neuropsychiatric disease- and brain cancer-risk genes have putative early roles in the telencephalic organizers' activity and across cortical NSC progression. Copy rights belong to original authors. Visit the link for more info Podcast created by Paper Player, LLC

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2022.10.03.510613v1?rss=1 Authors: Roberts, L. D., Hornsby, A. K. E., Thomas, A., Sassi, M., Kinzett, A., Hsiao, N., David, B. R., Good, M., Wells, T., Davies, J. S. Abstract: New neurones are generated throughout life in the mammalian brain in a process known as adult hippocampal neurogenesis (AHN). Since this phenomenon grants a high degree of neuroplasticity influencing learning and memory and mood related behaviour, identifying factors that regulate AHN may be important for ameliorating age-related cognitive decline and neurodegeneration. Calorie restriction (CR), in the absence of malnutrition, has been shown to enhance AHN and improve hippocampal-dependent memory, mediated by the stomach hormone, ghrelin. Intermittent fasting (IF), a dietary strategy offering more flexibility than conventional CR, also promotes aspects of AHN. The 5:2 diet is a popular form of IF linked to a range of health benefits, however its effects on AHN and spatial memory are not well characterised. We hypothesised that the 5:2 diet would enhance AHN in a ghrelin-dependent manner. To assess this, we used immunohistochemistry to quantify new adult-born neurones and new neural stem cells (NSCs) in the hippocampal DG of adolescent and adult wild-type and mice lacking the ghrelin receptor following six weeks on a 5:2 diet. We report an age-related decline in neurogenic processes and identify a novel role for ghrelin-receptor in regulating the formation of new adult born NSCs in an age-dependent manner. However, the 5:2 diet did not affect new neurone or NSC formation in the DG. Consistent with this finding the 5:2 diet did not alter performance on a spatial learning and memory task. These data suggest that the 5:2 diet used in this study does not increase AHN or improve associated spatial memory function. Copy rights belong to original authors. Visit the link for more info Podcast created by PaperPlayer

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2022.09.07.507054v1?rss=1 Authors: de Vincentiis, S., Baggiani, M., Merighi, F., Cappello, V., Lopane, J., Di Caprio, M., Costa, M., Mainardi, M., Onorati, M., Raffa, V. Abstract: Mechanical stimulation modulates neural development and neuronal activity. In a previous study, we proposed magnetic nano-pulling as a tool to generate active forces. By loading neural cells with magnetic nanoparticles (MNPs), a precise force vector is remotely generated through static magnetic fields. In the present study, human neural stem cells (NSCs) were subjected to a standard differentiation protocol, in the presence or absence of nano-pulling. Under mechanical stimulation, we found an increase in the length of the neural processes which showed an enrichment in microtubules, endoplasmic reticulum, and mitochondria. A stimulation lasting up to 52 days induced a strong remodelling at the level of synapse density and a re-organization of the neuronal network, halving the time required for the maturation of neural precursors into neurons. We then injected the MNP-loaded NSCs into mouse spinal cord slices, demonstrating that nano-pulling stimulates the elongation of the NPC processes and modulates their orientation even in an ex vivo model system. To the best of our knowledge, this is the first evidence showing that active mechanical stimuli can guide the outgrowth of NSCs transplanted into the spinal cord tissue. Our findings suggest that MNPs play an important role in neuronal maturation which could be applied in regenerative medicine. Copy rights belong to original authors. Visit the link for more info Podcast created by PaperPlayer

The National Counterintelligence and Security Center is among the many federal offices looking to shore up the security of U.S. supply chains. Jeanette McMillian is the NSCS's assistant director of supply chain and cyber. "Everything from software to semiconductors, pipelines to peanut butter, malware to meat packing, we have seen it all with regards to how global supply chains have impacted our lives," McMillian told me in today's show.

NSC's sind ein wichtiger Bestandteil im Rollenspiel, daher können wir nie genug davon im Petto haben. Heute präsentiere ich euch eine Methode, wie ihr anhand von Kreativtechniken ganz leicht NSC-Konzepte erstellen könnt. Ohne eigene Ideen! In wenigen Minuten!

Despite drawing criticism from Governor Pete Ricketts, receiving hundreds of emails, and hearing public comments for the first time, the Nebraska State Colleges Board passed two policies that protect against gender identity and more, late Thursday.

How To Buy NFTs What is a Non-Fungible Token NFT How To Make Your Own NFT Best NFT InvestmentsEpisode SummaryJoining us today for a SPECIAL episode of The Roadmap - Blockchain Boys Club!Blockchain Boys is a community for founders, developers, and investors to discover new projects, stay on top of blockchain trends, discover investment opportunities, and get quality trading signals.Check them out here! https://www.blockchainboys.club/Follow Blockchain Boys on Twitter!https://twitter.com/bcboys_nftChris KatjeMazhttps://bitclout.com/u/mazFollow The Roadmap on Twitter!Disclaimer: All of the information, material, and/or content contained in this program is for informational purposes only. Investing in stocks, options, and futures is risky and not suitable for all investors. Please consult your own independent financial adviser before making any investment decisions.Unedited Transcript:Hey everyone. Welcome to the roadmap. Benzing. As new NFT show, we've had some great teams on past episodes. Pixel vault, art blocks, the vole collective robots, the dos pound owning force, crypto dads, dizzy dragons, sub doc, and mobile. Today we have the team from lazy lions joining us on the 6:00 PM Eastern time, special edition of the roadmap.Stay tuned, everyone. We've got a great show coming up. This is the road.All right, everyone. Yet, if you did not hear me at the start, Benzing his new show, the roadmap covering NFT news and doing interviews with top projects out there. And we've got a great interview coming up to night. But before I talk about that, let me go ahead and bring on my cohost mass mass. What's going on, buddy?Yeah, what's going on? It's a pleasure to be here it's Monday. So it's throwing off stuff a little bit, you know, different time, you know, different day. Uh, it's going to be a great episode. Yeah. You know, we, we ere Tuesday, Wednesday, Thursday, 2:00 PM. Eastern time. We're three days a week now, but we had the opportunity to get this team on a stream live at night.And we said, of course, let's go because mass lazy lions. I mean, we talk all the time about projects. We talk about community. I mean, this is a big one and they're showing up in the chat. They've got 6,000 people in the discord, almost 50,000 followers on Twitter. And this isn't a new project. I mean, this minted back in August and this community is just getting stronger and stronger.Absolutely. So funny story, right. We first found out about this community when we shot an episode and then the comments, you know, they started, you know, talking to the cops. We tweeted something out when Zynga, I don't know if you remember that Chris and the crazy. So when Zynga is now, so, you know, thank you guys for tuning in.It's going to be a great episode. Yeah. I mean, the chat spoke, right. And also, I mean, Matt, as you saw it, right, we put out a couple of tweets sharing the preview of this video. Right. And the engagement on those tweets was insane. And that's how we know that there is going to be a lot of people here. A lot of people excited for this interview when we get that kind of crazy engagement on Twitter.So if you guys are new to the roadmap, please like this video, please subscribe to Benzinga channel. Again. We air the roadmap Tuesday, Wednesdays, and Thursdays, 2:00 PM. Eastern time. Mass before we get into the interview. I think we need everyone in the chat to roar. Right? I want to see some roars out there and we can play our trailer and get started with this interview again, mass, I mean this minted at 0.05, we've got a floor price of over one it's sold out in its first day.And again, this was back in. You know, so a different time than what we're seeing now. There come the roars here. Uh, yeah,go. And let's get those roars go. And guys it's Monday night here in the U S but I know everyone is joining us from different countries, different time zones. That's something we're going to get into in the interview later. I mean, they, they've got a nice base of users from different areas and I love that they break that down.Um, but yeah, I think we've got enough roars now, mass, what do you think that's run it. Let's hear it. Let's see the trailer.How about that at the end? Love it. I love it, man. I'm excited. Let's do it. All right. So joining us on the roadmap today, we have Asher the co-founder of lazy lions and nine. The developer got to go ahead, bring these guys on the stream. What's going on here.We've been doing that lately, where we do these trailers. We love putting those trailers out, you know, on Twitter, getting some engagement built up, but it's a great way to bring on our guests, right? It's almost like entrance music to play you guys onto the stream. So we're glad that you liked it. And we're super excited to have you guys on the stream and also this loyal community in the chat.Right.Super excited for this one. So, uh, yeah, that's definitely going to be exciting. And I just noticed now, as I'm looking at this. I came in a bit more casual with my hoodie and I see you've got the Riyadh Madrid shirt and all that.for this one. All right, guys. Well, let's dive into some questions, uh, before we get into Lacy lions, one of the first questions I always like to start with is. Tell us about your experience with NFTs prior to lazy lions where you collectors, where you investors, or was lazy lions, your first entry into the NFT space.Uh, let's start with nine on this one and, uh, hear from you. Yeah, sure. Um, so T is for us was very interesting actually, because when we first started, we were very into sort of like, okay, Um, so we used to play a lot of games when we were younger, um, in that. And then we start to learn about, you know, crypto.It first started when we got into cryptocurrencies. Uh, we got a little bit about that and it started to move into NFTs. I personally had an actual collected any of NFTs at the time, but it just tied in like the way that I understood it was like, oh yeah, it's on a blockchain, you know, proof of ownership.There was rarely factor involved. And that just took me back to our younger days when we used to play games and it just resonated with us and we spoke about it and we're like, yeah, this is, this is space. And when you were, and then we started to work together and get into the NFV space and start looking.I was building lazy lines from the ground up. Um, that's really how it started. I personally didn't collect any, I know Ash, I think he, I think he bought one, but yeah, he would touch on that more. Yeah. Ashley let's hear from you. Uh, what and FTS were you in before lazy lions? Yeah, for sure. Um, I mean, I just like to answer that question both.Uh, code up different things and then, um, you know, taken to mark. And that was always a lot of fun, um, with crypto, for me, um, I wasn't like a big crypto guy, like even during the off season. Uh, David, is that the lie that really interested me because I saw the value of the metal sets. It still makes a lot of sense, but it didn't hit me as like, oh, this is going to be a really big thing because I couldn't see it being like a cultural impact, but NFTs for me, it was beginning of this year.And as soon as I like started. To me, I'm like, this is not going anyway. Like digital ownership. Like we grew up, you know, playing video games where you've spent all that time and energy and money as well, but you can never take that thing outside of the game. And it's like, now you can actually do that.It's just, so for me, it's a no brainer. This is definitely not going anywhere. Um, one of my favorite entities actually like full AC lines. In our bungalow and a bungalow is an entity that we dropped where you can showcase your other entities. Um, it's actually forgotten ruins character. Um, and this probably ties in with what nine was saying, you know, would be gay.This girl playing greenscape loved that game and that little. Like, he just reminds me so much of my childhood. I tell you, I don't know how much that could go up in value, but I don't think I'm ever going to sell it. So, um, I guess a collector before, before those lines, if you want to do, yeah, it sounds like he's not going to sell it.Well, awesome love here in the video game. Talk as well. That's a common thing that we hear from different people behind NFT projects. Uh, you know, nine, you mentioned a little bit, uh, U2, uh, you know, getting involved together. Wondering if we can hear, or if we can hear more of the background, how did this team come to be and why the decision, you know, to, to launch lazy lions, uh, why a lion, why this type of artwork any a behind the scenes insight there?Yeah, sure. So it was, it first started. I think it was actually my birthday when we first started discussing like, um, cryptocurrencies and. We started getting into it. And we were talking just back talking about different coins that we're interested in. Um, and then later down the track, I believe it was Ashley that came up to me was like, Hey man, have you heard of NFTs?And I was like, yeah, I've heard of them. They pretty much just like JPEGs. Right. And he's like, yeah, but you can actually own, like, you could see these and there's a very flat line and it all started resonating for. And we're like, you know what? This makes sense. This makes sense to us being video game players, back in the days, it all like proof of ownership was a big thing for us being able to actually prove that you own this NFL, um, just made sense.Uh, it just made sense to us and we started just discussing it and we sort of just branched out to the team as well. And they all understood the. And then pretty much from there, we just thought, you know what let's just think of, um, think of the next best thing. And with that with us, we always went back to our heritage.So lions were pretty much a part of our heritage because of where we're from an student. So that just resonated with us and we just yet went full throttle with that. Yeah. I mean, just a few other things as well. Like the whole Kings and Queensland, hopefully my audio is working better now. I'm using AirPods and I realized it was a different setting.Um, like just growing up, but we've always referred to each other as Kings, right. Like even just with mates around. Um, and I feel like that's something that's just hit off for you. What would the community, and, uh, it's probably a small thing. Like they might be people in the audience outside of the lazy lines community.Going to their king or queen, but I think it goes more than that. Like it ties in with that values, which is yes, we're all Kings and Queens, but no one's above anyone else. And I feel like that's something that resonates with a lot of people in web three. Um, so yeah, just a few little things like that. A lot of that.So guys, so the floor is over one eighth, you guys have over 86 K into. And the 49 K on Twitter. So how do you guys personally measure the success of this project? Uh, let's start with you Asher, if you can tell us a little bit more. Yeah. You know, it's, it's interesting. Like there are a few different ways you can, you can look at it.Um, and that, and that we do track, right? Like obviously we've got our own KPIs that, you know, we had really, really smart people who know the ins and outs of, um, the whole economics behind it. Yeah. Tell us what metrics we should be looking. Um, and the community knows that, um, it, it ties in with down mission, which is simply it's to become the, the biggest and best entity community there is.That's, that's our mission. Um, obviously we're a bit biased. We think we've already got one of those. Um, but to be the biggest, it's very black and white, so that's what we're aiming for. Not to put anyone else down. I, I love some of the other projects that are out there, but that's just our mission anyways.Right. Um, so that's one way that we track success. Um, That's small quantitative like qualitative, just speaking with community members, the five, particularly in this dip right now. I feel like for me, um, that's what really just reassures me that we're on the right path. Just, you know, you jump into the discord, you see what's being posted.You have chats with community members. I try to say. Um, spend a fair bit of my time just having those, like one-on-one chats with community members. Um, I feel like that's a really important thing. Like just, um, like really like, Hey, like how are things going? Like, you know, what do you feel is blocking us like real deeper conversation?Um, and that's just been like, it's not obviously like all positive, like they're always things that we can be improving on, but overall it feels like we're on the right track. So that's, that's the way we measure it. Yes. We've got data that having those conversations with community members, um, tells me we were on the right track.Love that. Yeah, same. I like to hop in a discord and you know, you see the activity level. You know, there's been so many discourse throughout this period that are just dead, you know, cause it's, we're kind of in a bearish market, so there's no one, you know, having a conversation, seeing you guys have this activity is amazing.Uh, how about you, uh, nine? What, what, how do you measure the success of the. Yeah. So similar to what, similar to what Ash said. I think for me personally, it's during this fast success of the project, just jumping into the discord and seeing how active it is on a, on a daily basis, being able to hold these, um, conversations with, you know, my team members and, you know, moderators and community members.And they're all, we're all on the same page. We all understand what the, you know, what we're here to do. And just, yeah, just seeing how active for me personally. Yeah, more on the community is just how active it actually is. And seeing the word being spread out, spread out on Twitter, spread out in discord.That really just resonates with me. So I love that. So can you guys tell us a little bit more about bungalows and how they, uh, you know, come into the lazy lion world? Yeah, absolutely. Absolutely. Um, and so I guess when we started, right, like we had a few things, um, In mind for the roadmap. Um, and two, we didn't want to be different just for the sake of being different, but at the same time, it's like, it was very obvious to us that if we just, I guess, copied what other projects were doing, like we could find our own way.I think that that was a bit lame. Like that's not what we're about. Um, we're in a new space. I think there's a really good opportunity for us to build something unique and that's fun to us. Right. And it's something that was that we noticed during our research of NFTs, like at the beginning of the year was like, how do you show these things?Yeah, that was the question I had. Um, and I spent a fair bit of time looking into that. I still feel like there's no great solution, um, in real life, if anyone has something like, you know, a great way to like show them off in frames or whatever the ambulance put down, I'd love to know because I want to bring out obviously my line, um, and a few other odd pieces.Yeah, that's in the real world. And, um, you know, in the digital world, you know, how do you show these things off? I've seen a few like, um, social media websites, but nothing great. So just to us, it was like, well, Twitter event is on being used profile photos are what if we could show off entities in the Twitter banner, um, and you know, sticking with our values as well, which is to be inclusive rather than being exclusive, which I think a lot of.Projects really focus on, you know, they're trying to build this exclusive community, um, and that works for them and that's great, but where we're different to that, you know, we're trying to, you know, we're aiming to be inclusive. So, um, sticking with that nature, it's like, you don't just have to show off your lazy lines indices, um, without new bungalows DAP, which is on all these loans.And if the.com you can edit any bungalow, you have to include any NFT that you weren't. Even if it's not lazy lines for later. So, um, yeah, that's, that's how it works. It's a bit of background as to that's awesome. So me and Chris liked to ask this question on a tour guests, every episode, what are some of your favorite traits from the lazy lion?So are there any unique traits that you particularly liked that no one knows about let's start with United. Can you tell us a little bit more, you know, which lions do you like the best or what traits do you like the most. That's a good question. That's that's a great question. Um, which ones are the ones that are being slept on?Let's let you will personally love, you know, uh, honestly I think I used to love the, I like actually like the, really like the lederhosen, um, Um, it is one of the ones that I had originally as my PFE or my line had it. You had the, um, lederhosen shirt out of my eyes and I believe it had the halo just look very innocent.So I was like, yeah, this one, this one resonates with me. Um, but I think that one's a little bit slipped on as well. It's, it's, you know, it is one of the real ones as well, but you don't see too many people will actually actually with it. Sowhat about you Asher? Um, there's a couple other big. Um, a big party, that guy, just because of the whole childhood thing, but, um, basically these, uh, these leads of fire, I think. Um, and I know I'll get in. Um, I'll get a bit of stick if I, Aaron. And he's part of that team as well. He is a community manager. I know he loves the lazy hat.Um, so that one should probably get some recognition as well. I think that one's probably a fan favorite. Yeah.And some call-outs for us steak mouth in the chat as well. It looks like there's some fans of the meat and the steak out there in the chat right now that guy's going to get about 50 steak bouts. So that's why, that's why, that's why. So there's a little bit of a bias going on. Oh man. Well, Sorry. There's a little bit of a little trait combo that I like as well.So if you have like a, if there's a scratch body and they have a ripped shirt, you can see the scratches underneath the shirt, which is what just comes out really nice. So it's a little thing that I sort of go for as well. I like that. Nice, nice call out there. What about you guys? You know, I see in them, I like the steak right there, you know, and again, I know there's someone in the chat, you know, bias towards that, but I like the steak.I like the party hat. I know there was the halo, um, you know, some of the different, uh, the headpiece traits. Um, I like, but I also have seen some real clean ones. Uh, you know, that are impressive. Uh, what about UMass, um, football Jersey, you know, big football guy. And then I do like, so I do like anything Miriam based from any collection.I'm always trying to find like, is there an Ethereum chain or anything like that? I saw you guys having a theorem business shirt. I like that one as well. So yeah, exactly that one right there. So always those little subtle pieces. I think that's cool. Definitely. All right. Well, you guys also have roar words for your owners to reward the community members.Can you tell us a little bit about this? Yeah, absolutely. Absolutely. Um, this is probably what been one of the biggest, um, I guess catalysts of the project, particularly at the beginning, uh, at the early stage of, sorry. To put it simply, uh, rewards is what it sounds like it's a rewards program exclusively for lazy lion holders.So this is one of the advantages, or I guess, utility of being a lazy lion NFT holder. Um, every month you're eligible for rewards in the form of eight by completing activities that, uh, That benefit the project. So, you know, community engaging activities and part of those, you know, they change from month to month, but one of those might be, you know, sending your profile photo on Twitter, to your lazy lion and they, um, and you know, I feel the little things like that.And that might be why you see, you know, quite a few, um, you know, about how those changing their profile photo, um, to the lines, particularly the, the early stages, but what we found now, it's really interesting. Right. Um, And I should probably explain why we did this, but what we've seen is like, it was a good reason.It was a reason why a lot of, um, people I guess, came to the project. Um, but what's happened now is like, it's become so insignificant where they just, they love their lines so much. They don't really care about rewards anymore. Like, that's a lot of the feedback that we get is like, yeah, that was really nice at the beginning, but it's like now I love my profile photo so much.I'm not gonna change it. Even if you would've paid me to. So it's like, it's sort of changed, um, how they, how they view it. Um, but I think it's important to like, to explain why we did this. Um, it's sort of like a marketing expense, I guess if we, if we look at it that way, like from the project's point of view, like I know a lot of other collections and then this isn't me bashing anyone else.Go down the route, which is the easy route of, you know, paying like an influence it's changed their profile better and, you know, paying a big amount, a large amount of Psalm, one influence, a change, that profile photo. Great. We saw that as a bit cringe and we're like, is there another way to do this? Um, but obviously we still need to do marketing.Like obviously the project needs to be marketed and it was like, what if instead of just giving it to one celebrity or influencer. What if we just give that to the community and instead of just one big amount to one person, we just spread it out to the community. I'm like, well, that ties in with the whole, you know, web three N model.So that's how we went. And we just created a system that allows us to do that. Essentially the community gets rewarded for, um, marketing the, the project. And I feel like that's worked one just for us and. Yeah. I mean, that's my, that's my opinion, but I think, you know, if you jump on Twitter and you just look at all the activity from lazy lion holdings, it feels like it's sort of evolved beyond that as well.This is awesome, dude, this is some alpha for anybody looking to start a project. Seriously, notice that there's your marketing right there. And if you're looking to create an NFT project, yeah. I mean, what a great way, uh, love that. And, you know, look at the numbers you guys have too in building the community and the community.I mean, being so loyal months after the launch. So clearly that rewards is worth. Um, I saw a picture on Twitter that you guys had a meetup in New York city, some lions holders. Um, were you guys involved in that? Have you met any, uh, lions holders and, uh, you know, talk to us about future events for lion? Hey, the Kings and I wish we were there.So where, um, we're still in lock down here and study CD. Um, so I think we just now started to get out of lockdown, but as soon as we can fly out, like desperately, we'll be meeting up with laser line holders, but no, that was community led. Um, a lot of, a lot of really good feedback actually from that one.Um, everybody seemed to really enjoy themselves. Um, but yeah, that was a little community, but we'll definitely look at doing more of those. Um, as soon as we're able to fly out. That's awesome. I mean, showing the initiative by the community there, I mean, that's something we don't see out of every project, uh, doing these, you know, these meetups.So a, another great sign of how big this community really is. Um, I did see, you know, that there are quite a few celebrities that also own lazy lions. Um, that's something we talk about a lot on the show. A lot of athletes does Bryant, Josh Hart, Seth Curry, uh, Tyrese Haliber and also we've got Jake Paul John rule.Uh, talk to us a little bit, you know, I know you guys didn't go after all these celebrities, what does it mean to see some of them get involved in the project in terms of validation going forward? Man, it's really cool. Uh, it's really cool, but. Uh, just in general, just with the community, like what I know it's sort of gone away from the question, but just what we love, 4,600 holders, like jumping into the, or like sometimes find him, must find myself just going like, well, is this sort of like a dream?Like you sorta just have to step back. You're like, am I just going to wake up? And that damn like, went back in August, this never happened. Or just, you know, continuing on with whatever else we're doing. Um, so it's sort of surreal like that just with the community, which is a really cool thing. But, um, no, I mean like the celebrities in the.Uh, the athletes, um, yeah, that's been organic or organic. Sorry. I'm like nothing from us anyways. Like the, the teams specifically bringing in those ones. Um, but yeah, I think it's really cool. Like it just goes to back, I guess they believe in either the project and the community or, you know, it's probably because they resonate with the art as well.Um, like from a few of the things that I've seen. Um, which I guess makes sense. You know, everyone feels like a, you know, a lot of people feel like they're aligned, particularly athletes. Uh, um, yeah, it's it. Awesome. I got a highlight Tyrese Halliburton for a minute. Cause we talked about him last week on the show because the big news was that he, uh, wore a pair of sneakers during an NBA game, featuring his board ape on his shoe.So my question for you is, you know, has there been any talks of Tyrese Haliber and having his lion on his shoe? Is that something you guys would love to see? And what has the reaction been from the community of that possible? I think that's so cool. Like now that we're seeing it, he's going into a culture like that.Yeah. That's um, that's awesome. Um, I got a lot of respect for board apes as well. I mean, they've sort of paved the way for a lot of projects out outta here right now. I think it'd be really cool if you wanted to do something like that. And with the lazy. I mean, I don't know. How's the community feel about that when you recognize that guys in the chat, let us know.I mean, I'm seeing a comment here when lion shoe, uh, you know, is that something you guys would love to see an NBA game with lazy lion sneakers being worn? Uh, you know, I know we got a ton of press about the board ape shoes last week, and it was really cool to just see an NFT, you know, on a shoe in real life.The screen, um, you know, and Burton's changed his shoes, uh, you know, pretty much every game. So I have a feeling, you know, that he's not done and there's his lazy lie in there on screen. So, uh, can we get that on a shoe? I think that'd be pretty sweet. Yeah, that's awesome. You called it Chris. Let's see. Let's make it happen.Let's make it happen. The crowd would be roaring. David says great comment. There. All right, guys. So back to the discord really quick. So again, you guys have over 80 K discord members, you know, I'm sure we've all been crazy. Discords where they're spamming and just out of control. So what do you guys do to manage the discord?Yeah, we've got a great team, great team. And that's, um, you know, not just, uh, uh, just the community managers that we have in there, but even the moderators as well. And I think anyone in the community with. Um, you know, a lot of people I could shout out individually, but yeah, the modes, the community managers, um, support team, and even just like the w we've got like different roles.Like we have one called like a role, uh, Royal king, sorry. And these are like community members that are sorta like self appointed by the community itself. It's funny. It's like, we don't even choose. It's like, it's very obvious it should be this role. And they're the ones that are sort of. The community outside of the actual moderators.And I feel like the community itself just does a great job of like, self-governing like if somebody comes in and sends somebody that something that they shouldn't it's very quickly resolved by itself, which is just a beautiful thing. Um, so it's like, even though we have, you know, the more and more we scale up and grow, um, it hasn't been too much of the.Um, but yeah, that's, that's how it sort of does it, so it doesn't get, like, it gets crazy at times, for sure. Especially on like, you know, Lauren's days and like rewards, especially. And we've got that one coming up this Friday, um, when the, uh, Downey's is sent out, you know, it gets pretty dense pretty well of that day because it's a bit of fun, but, uh, now it's all governed quite well.I think. Um, that's awesome. How active are you guys? Both in the discord. And do you have any like favorite channels in there? I'm probably a bit too active in the, um, in the disc, like considering like, um, also, um, like leading up like the product team and obviously like, uh, just trying to keep my eyes on everything else in, uh, in the project.Um, but man, it's just so addicting being in the discordant. Like what do you, I guess it's just like, it's hard not to be what up in the, in the moment. And then you're sitting there for like half an hour, just chatting back and forth with everybody in the. And the community. I think we get FOMO too. You want to scroll back and see what you've missed.Finally, you said the scrolling through your phone for a little bit to try to catch up with everybody and what they're saying, but in terms of favorite channel, I would have to say it's probably, um, it's probably, it's probably cabana bar. That's our, that's our main channel for lazy line holders. Um, so it's sort of, you know, it's only available to them.Um, but when that channel starts to pick up, it's really fast to pick up on. There's a lot of great discussions that, um, Uh, so yeah, you learn a lot and it's, it's great. Speaking of the community, uh, specifically lazy line holders and then, yeah, that would be probably my favorite channel. I'd say there's lots of London.Yeah, love that. So we've seen a lot of discord hacks over the last couple of weeks. You know, this court, Twitter hacks, you know, different kinds of schemes going on there. Are you guys taking any precautions towards, you know, protecting the discord? Uh, let's start with you. Yeah. Sure. So recently we've done like a, an audit of our bots just to ensure that there is no, no left over bots that are lurking, that we sort of don't need.Um, majority of our web hooks. I know they can be a little bit risky, um, in regards to, yeah. So I know that one of the discourse I apparently got, you know, got it. They got into it because of a web hook. So we started to, you know, just review the web books, make sure they're all clean. Make sure there's nothing we don't know about.Um, we've started to leave sticky bots, uh, sort of sticky notes in each of our. I know that sometimes you can get DMS from fake collab land when you try to verify your NFTs. So we have sticky notes that, um, you know, just say, make sure, like, just confirm correct collab, land, which other scans, it leads the country, uh, of that channel.So nobody can see like, so no collab then can sort of see who's typing, you know, exclamation mark join to verify that. And if they, so just little things here and there to really just clean up the discord, make sure there's nothing that's sort of. Leftover to ensure the safety of the community members.That's, that's what we've been doing. And we'll continue to automate it just to make sure that nothing happens. Yeah, yeah, yeah. Pretty much that. And um, I mean, cause our discord is like so important to us as well, right? Like it's such like it's front and center of our. Um, like the project strategy, right?It's like, that's what we're really trying to grow and build a community. Cause like we've always said that like the project is the community, the community is the project. And like we're making a, we're making our bed on the discord platform. Um, that's not to say it's perfect. Like I feel like there's, you know, there's a few things that I, you know, I'd love to suggest to the disco, um, team members and just, you know, things that I think would benefit the platform.Um, Overall, particularly as like entity and crypto is becoming more and more of a thing that's using discord. Um, cause like we've let so many important, um, positives, like we've got like a professional networking section. Um, cause it's funny. It, it goes, it's sort of gone away from the question, but it sort of ties into like what's the value in an inequity?Um, like that's a question that I've, you know, I had asked, uh, a few times basically for people that aren't in crypto, like why would you get an NFTA? Like what do you actually get out of it? Yeah. Uh, like one of the most obvious things is the community. And some people just look at that and go, oh, like, what is that?It's like, well, like, you know, it's just because they're cartoon characters. I think the stigma is like, people think it's it's little children or like fat kids in their mother's basements that are buying these entities. Right. And it's like, that's not the, that's definitely not the case. Right. Like, Like we're speaking with, you know, with like C level executives with doctors, with lawyers, like whatever their industry they're in, you know, our reporters, like people at the top of their industry, right.Are like really smart, intelligent people. So it's like, um, how much does it cost to get into like a professional networking? Um, uh, you know, like, oh, like, you know, how will, what's the cost of that to be a part of that? Create those kinds of connections. Right. And that's something we've doubled down on. So we have like a whole professional networking section and in the discord, um, like we have gaming sections, um, marketplace, um, like all these other things.So it's really critical to us. And security is really important as well. Um, those hacks are pretty scary, so it's just things that were, yeah. Like , those are some of the things that we're doing to make sure that, that Allstate. Uh, secure love that. So you actually just led me on to the next question I had for you guys.I'm going to have a list of pull up this chart here. So someone posted this in the tweet and the tweet last night, and it's interesting because it breaks down all the demographics, right? So there's, you know, 4.6 owners. Yeah. So this is really interesting, right? Gender, you know, 92% male, 8% female, top three age groups, 26 to 35 leads.Uh, 36 to 45 is second. See, I wouldn't have guessed that 19 to 20 five-year-olds is third. Uh, this one to 53% are married. 44% are single 60%, no kids, 33%, one to two kids. Can you guys tell us a little bit more about all these stats, man? This is really cool. Yeah, it's really interesting. I think some of those numbers would love for them to be a little bit different, um, particularly cause we have.I think if you compare that to the average would probably be a bit better. It's basically in terms of, you know, having more or, um, more females in the, in the project being inclusive is obviously one of our core values rather than being exclusive. But this was just part of a survey that we, we did a while back.Um, Uh, yeah. And it's interesting, like target market is, you know, 25 to 45 year old males, obviously high disposable income as well. Um, but, um, yeah, it's definitely interesting. We thought other brands would be, you know, we'd love to see that kind of information, particularly now as we've got the collectibles, um, you know, probably sneak, peeking a bit too much of that.Um, but just to look at about that, but that's something that we've got coming out. Um, Quite soon. Uh, and that, I think it's an opportunity for, you know, we've had so many requests to do like collabs, um, you know, so many projects that have hit us up and we're like, Hey, can we do something? And we just haven't done anything with them strictly because we just haven't wanted to pay like any influences.That's not been our strategy on the really cringe. Um, it just goes against that values. So this is an opportunity for us to actually partner up with brands in a meaningful way. So that's why we shared that. That data. Um, and I think it's quite valuable daughter to Danielson and entities, but yeah, that's, that was the reasoning behind it.Awesome. So one of the things we've talked a lot about on the roadmap, obviously NFTs have seen huge interest this year. And we also have news that Coinbase a leading crypto platform is launching their own NFT marketplace. So they actually pulled their audience asking for favorite NFT communities. And of course the lions community.Came through and roared and Coinbase actually retweeted. And they said roar louder for the people in the back. Please. Uh, tell us what this means. Uh, you know, have you been in talks with Coinbase at all or is this just validation that Coinbase sees the large community here? Um, that's one of those things where I probably shouldn't say too much, maybe just because I can't say too much, but I think that might be enough there just to say that you can't comment.So maybe we got it. We got it out of here anyway. But I think it's really cool. All right. I think it's really cool and it's really exciting time. So we'll see what, um, you know, we'll see what happens with the platform and then we've all got a lot of hopes. Um, but at the same time, you know, like we've got to be a little bit skeptical.Like there's a bit of pressure on them to make sure everything comes out. Well, it's not a guarantee or anything. Like we saw what happened with like Google plus, right. Um, just because you're the biggest doesn't mean it's going to be a guarantee, but, um, I think the fact that they moving into NFT.Entities and they're focusing on it is extremely bullish, right? Um, so I've got, I've got high hopes and they got my support. Love it, Google plus. Wow. Take taken me back there. I definitely, I definitely had a Google plus account where I would share links to articles that I wrote to try to get increased page views, but yeah, goo Google plus a diet and unfortunate death there.But yeah, that's a phrase I haven't heard in a while. Um, I think we have AR in the chat and I believe there was a mention of a tattoo. We do have a picture and a video of this tattoo. Uh, again, we, we talked to a lot of NFTE teams, right? And w we occasionally see strong engagement and we do not see too many tattoos.So tell us a little bit about, you know, uh, why this tattoo came to be, um, looks like AR you know, called out for the floor price and then got the tattoo. Is this something you guys do about at a time and how did the community. Uh, man, I think everybody loves it. Like look at how clean that thing. Like man, whoever the tattoo was like, yeah, he's got some really good work, but um, no that was all right.That was something he called out back when we first, when we first launched it, I remember seeing that and it was like, why did he say it's like, when were you reached to a, I'm gonna get my live. And I remember looking at that and like, obviously, like I'm extremely bullish on what I've got completely.Like from the early days, I was like, sure, like, you know, we can do, we can achieve whatever we set our minds to. Right. And I still believe that. Um, so I was like this, guy's going to have to get a tattoo of his life. That is a sweet looking tattoo. Uh, I mean, loved watching that video. So clean, so shout out to the artists too.Um, but yeah, I mean, seeing someone get a tattoo, I mean, that's great validation for, for the longevity of this project and the community. So, uh, an AR in the chat, I mean, a nice looking tattoo and thanks for joining us for the stream today. Uh, along with the tattoo talk and validation, another area where we've seen NFT projects, you know, connect with their community.As we just had Halloween and I saw some lazy lions, Halloween costumes. Um, you know, is that something that you guys, uh, you know, called for, did a contest at all? Or is this just people happen in because they wanted to dress as their lion. Yeah, this was just, this was purely community-based. Um, when I actually saw that image and retweeted it on the main Twitter account, that was absolutely awesome that people were getting so involved.I really liked it. I'd love that lazy hat as well. It looks so clean. I want it personally, but it was, yeah, that was just completely community driven in hold a competition. They just went out of their way and yeah, it's just beautiful to see. That's awesome. Uh, yeah, I mean, what a cool Halloween costume.That is a sweet looking hat too. Um, but keep that in mind, any lions in the chat, if you're looking for a Halloween costume next year, I know it's a year away, but start planning to work on your lion costume now. Um, so you can be ready. Uh, you mentioned a little bit about collectibles, right? So we got a little bit of a teaser.One of the things we are called the roadmap after all is we always try to hear about, you know, what's next. So I know you guys can't share too much, but can we get a little bit more information on collectibles and maybe what's ahead for lion holders. Nine, if you don't have a problem. I mean, why not? Like I've got a lot of respect for this show, so it looks sneaky.All right. So essentially this ties in with our values and our mission as well. Right. Which is obviously like our value is one of our values is, you know, rather than being an exclusive community, which you don't know a lot of. Uh, you know, focusing on being, which is fine and like that works really well.And I've got a lot of respect for a lot of other projects. I would never bash another one, but I'm like ours is to be inclusive rather than exclusive. So, um, we're trying to build obviously the biggest and it's the community, that's our mission. And part of that is bringing new people that aren't necessarily in NSCS right now.And I feel like we sort of shoulder that responsibility as we've become. Um, you know, just from what other people have told me, it seems like we were kind of one of the leading projects now, at least like in the top, you know, I think like on open seat, like top 40 or all the time traded those types of things.So I feel like we have a responsibility to actually, um, you know, to try and lead the space and, and bring in new people into the community rather than just, you know, those existing members. We already have enough teas. And I think the challenge is. Because it's still so early, like the interfaces and everything, it's not super easy to get into it.Like know just the act of having to create a wallet and minting and gas. It's sort of like, you know, I said, it's like, if you're telling a friend or family member of someone that you know, coworker about entities and they're not in it already, it's like, Hey, we've got a lazy lion. This community is amazing.Oh, cool. How much is it? And he's like one eighth and I look up one eight. They're like what? That's not how I'm going to get started. Right. So it's like, okay, how can we say. I have those, um, people still become a part of the community without having to fork out that much straightaway. And that's where this sort of referral program collectible thing came to mind.So essentially what it is is every community member or every new person that you invite to the community, all they have to do is join discord and they will get a pack of tokens for free that they can claim on our website. Um, and this pack will be a random, you know, it'll be a set of cards. Um, you know, like we had to have a lane, next one will be, um, uh, like, uh, Thanksgiving.Right. Which is what we've got planned. So that's probably a sneak peek there more than I should. Um, so there'll be, you know, a set of eight, eight, uh, Thanksgiving cards. Um, and every pack that you open, you'll get two random ones. And the idea is complete the set and you enter to win a lazy lions. Um, so this way, anyone who's not in the community already can just join up, join the disco and get a pack.And if they want to get more packs, they just invite more people. And we cover the gas company. These tokens appear on open. See they can list them free without having to pay any gas that's all covered. So someone could come in, not pay a single cent, get one of the rare tokens, list them, and then, you know, make a profit or try and complete the sentence, win a prize.Um, and I think it just ties in, like we said, without values gives them an opportunity to experience what mentoring is. Um, and then, you know, bringing in the ecosystem and understand the true benefits of web three. So. A bit of a lengthy explanation, but yeah, that's, that's what the collectibles ideas. And what does that mean?We appreciate the background there. Yeah, when's the launch. Yep. So right now that is in, um, if we've got like a soft Bader internally, um, that's scheduled for launch, uh, next with next week. So we've got rewards this week. Oh, wow. Is that a big thing? And then we've got this coming out next week. That's awesome.That's awesome. Yeah. Yeah. Yeah. So yeah, pretty excited. You'll definitely see. Um, I think this will, yeah, we'll see how it goes, but I can see it having a pretty positive effect. I think so. Yeah. I, we we've seen some of these card ones do well too, and that's a great way. Like you said, to bring new people into the community and also the referral program, uh, you know, I'm a big fan of that.So. You know, uh, we wish you luck with that. And thanks for breaking that news here on the roadmap, it's always exciting to, you know, hear what's next from these projects. And, you know, I think that a community out there is loving this. Absolutely. I mean, that's amazing bringing new people in is the biggest part of the game.That's that's really awesome. Uh, let's take it to, we have some questions from the chat here. Um, the first question that I saw earlier in the interview today, we had a question from Brendan bald. Uh, he's asking, do you guys have any plans or hopes to develop the IP into film or TV or even comic books?So the cool thing is I've already seen a few projects do it. Um, and I think they're just, you know, they hold the, uh, lazy lines and if feed themselves, once you hold it, you're able to go and create these things yourself, um, are stressing. Like we're open to it, but there's no immediate plans for it, but I've seen a few cool things going around on Twitter.Yeah. I love that. Um, let's see. Do we have any more questions from the chat here, Chris, that we want to think we got most of the questions answered. Well, you know, the, uh, people loved hearing the Coinbase conversation again, and people loving this roadmap talk. Um, we're seeing merge now asked from Morgan.Um, you guys, I think you, do you have some merch already or ends for Merck in the works? Uh, we've got much in the works. So, um, you know, I've seen, what's been being worked on, um, I think the community's going to love what we, what we release with this. Um, so yeah, not, not quite yet, but that's in the works.It's coming out soon. And then mass. How about that? A question there from a breezy in the chat, uh, that, that could be a fun one. Oh, yeah. So when Arizona ice tea collab, can you guys tell us more about that? Is that a rumor or, you know, is this question coming out of nowhere? Um, yeah, I mean, there's nothing like official going on.Um, what's nice. Is it sort of ties back into the collective. Um, to is now like, again, if there are any brands listening as well, I feel like there's definitely an opportunity for the brands who are wanting to get into entities, particularly with like more established projects. Um, at least for us, this is an opportunity for us to actually partner up with brands in a way that's actually beneficial for everyone involved.So, um, you know, if we were doing those, you know, biweekly, um, releases of collectible cards and one of them. The Arizona theme, just for the sake of argument. Um, but yeah, not nothing official, but we're definitely open. Perfect. Yeah, I did see Arizona as one of the owners mentioned in the, uh, celebrity spotlight, um, on that cool graphic and the facts that we shared.So I think that's where maybe that rumor comes from. Um, but we'll wait. We'll wait to hear more details from you guys in the future. Well, I think that's going to do it for today's interview, you know, nine Asher big. Thank you for both of you coming on our show. I know it's early morning where you guys are at, um, your flexibility with doing that.Also shout out to the latest science community. I mean, mass, look at them. They're in full force here in the chat during this whole interview. So a shout out to lazy. Absolutely. I mean, this has honestly been one of the biggest communities I've seen and I'm personally measured by the Twitter. You know, I see on my Twitter, the profile picture, I mean, I see a lie and I would say one out of every 10, if that, I mean, you guys are killing it.I can't wait to have you guys come on again and just, you know, do some updates in the. Yeah, man, this is a lot of fun. Um, I have like, everyone's been waiting for it for a while. So this is tape spot. It went by a lot quicker than I thought it would. Alice. That's how you know, but, uh, yeah, not, King's definitely out to doing this.Yeah, well, we will, we will have to have you guys back on. Um, so thanks again, nine and Asher, uh, before I let everyone go here, uh, mass, we do not have a show tomorrow. Um, I know I said we're going Tuesday, Wednesdays and Thursdays, 2:00 PM. Eastern time. Benzinga is hosting. PAC awards. And actually there's going to be some awards given in crypto, in NFT categories.So if you're interested in the space, we have FinTech awards, airing live on YouTube, beginning at 9:00 AM, Eastern time and going all day. And also if you're into crypto, we do have our Monday night show. After this show is done with, through there, he will be talking some metaverse crypto plays. Um, so I know I'll be sticking around to hear more from Ruelle on.But yeah. Thanks for everyone for tuning in and yeah. Thank you. I mean, let's chat, they're so appreciative to mass here. I am trying to tell them. Thank you. And they're all thinking. Yeah. Thank you guys. If you guys liked this content, you know, we're, Benzinga, there's there, we're starting to test this out.We want to bring more NFT content, make sure to like subscribe, share this content. We're going to continue to bring more of it as the NFC community expands as well. So thank you guys for watching. We appreciate. Definitely. So stay tuned, everyone for a root welds report talking crypto plays, and we will see everyone on the roadmap on Wednesday, 2:00 PM.Eastern time. Take care, everyone. And thanks again.Support this podcast at — https://redcircle.com/the-roadmap/donations

Kommunikation ist ganz schön schwer. Und gerade im Rollenspiel führt es oft zu Problemen, wenn die Kommunikation zwischen Spielern und SL (oder den Spielern untereinander) nicht ganz rund läuft. In Folge 9 besprechen wir deshalb die Bedeutung von klar lesbaren Beschreibungen, Charakteren und Spielsituationen. Kommt in unseren pädagogischen Stuhlkreis - den Vorsitz hat unsere alte Bekannte Miss Verständnis! 00:00 Hail and well met 01:01 Was bedeutet Lesbarkeit? 07:20 Vaylans Strohmann-Parade 17:36 Pacing, Stimmung & Regie 36:02 NSCs & Kämpfe 53:56 Schwierigkeiten & Detailgrad 1:07:31 Fazit Musik-Credits: River Valley Breakdown by Kevin MacLeod Link: https://incompetech.filmmusic.io/song/4295-river-valley-breakdown License: https://filmmusic.io/standard-license https://www.youtube.com/channel/UCY-YM2jeo8-x4q5G7JyNXtQ

The Wendy Love Edge Show with Topher Kogen does not dispense medical advice and all your health choices are your own. Guests: Pot Brothers at Law Marc and Craig Wasserman. http://www.potbrothersatlaw.com/ Kevin Kennedy: Casualty of the drug war and former plant prisoner Find him on FB https://www.facebook.com/groups/321371855600475 James Russell III Gubernatorial Candidate for 2022 in Arkansas https://vote4rus.com/ Nico CASH-N Is a songwriter/ entertainment industry professional from Houston, Tx. Volunteer mentor to students through Texas Southern University's NSCS chapter. Houston Community College Alunmus. Nico Cash-N is the founder of CA$H-NTERTAINMENT which has produced various music productions and have assisted in the production of six short films. He seeks to produce powerful content to help influence the world through various forms of art. This show is written and created by Wendy Love Edge and Topher Kogen Show Management A. Edge Productions Producer: A. Edge Productions Editing: Flint Woods Booking Music and Art: Mike Kinkle Join us on Patreon: https://patreon.com/thewendyloveedgeshow Sponsors: Relevnt 131 Inclusion Gallery Karas Healthcare Purely Natural CDB Highlands Residential Mortgage Uptown Kitchen and Taphouse Mary Will Nourish KJ's Wellness Arkansas Hemp Genetics Opalstack --- This episode is sponsored by · Anchor: The easiest way to make a podcast. https://anchor.fm/app Support this podcast: https://anchor.fm/thewendyloveedgeshow/support

This episode talks about the how to shop online safely.  After listening please visit National Cyber Security Centre, Action Fraud, Take Five and the West Midlands Cyber Protect Websites for more guidance on all things relating to online Security.   To learn more about typosquatting visit the NSCS and this article. Please forward any Phishing emails you receive on to report@phishing.gov.uk and any text messages on to 7726. Our hosts today were Patrick, a Detective and Cyber Protect officer for the West Midlands Regional Cyber Crime Unit (WMRCCU) part of the West Midlands Regional Organised Crime Unit (WMROCU) and Demi the WMRCCU as an intern taking a year out from her University Studies. Also, a member of the WMRCCU is Sean Long – WMPDigitalPCSO – West MidlandsJames Squire - cyberpcsoWarwickshire and West Mercia   To contact us please email us at wmcyber@west-midlands.pnn.police.uk to signup for our newsletter please visit www.wmcyber.org/subscribe. 

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2020.11.10.374876v1?rss=1 Authors: Olmsted, Z. T., Stigliano, C., Scimemi, A., Marzullo, B., Wolfe, T., Cibelli, J., Horner, P. J., Paluh, J. L. Abstract: Improved human stem cell interventions to treat CNS trauma requires continued expansion of in vitro models and delivery platforms to fill gaps in analysis and treatment. Transplanted neural stem cells (NSCs) face unique, multi-faceted challenges beyond survival that include differentiation, maturation, and integration into a complex cytokine-releasing microenvironment that impinges on a multipotent cell type. Alternate strategies to transplant neurons and neuronal networks deserve reevaluation, particularly since novel differentiation protocols mimicking region-specific developmental and positional cues have recently emerged. To investigate transplantation of neurons and their early networks, we generate in vitro neural ribbons containing spinal neurons and support cells anatomically matched for cervical spinal cord injury (SCI). These glutamate-responsive, electrically-active neural ribbons apply a new hiPSC differentiation strategy transiting through neuromesodermal progenitors (NMps) to derive developmentally relevant spinal motor neurons (SMNs), interneurons (INs), and oligodendrocyte progenitor cells (OPCs). Bioinformatic profiling validates region-specific identities. Neurons and neuronal networks are functionally evaluated for action potential firing, calcium signaling, population activity, and synaptogenesis. NMp-derived neurons survive in vivo within the subacute phase hemi-contusion injury cavity when delivered either as free suspension or as encapsulated networks of pre-formed CNS cytoarchitectures. Delivery as encapsulated networks further supports survival of lower cell numbers and rapid graft penetration into host tissue. Neural network ribbons therefore provide a novel intermediary approach between cell suspensions and complex organoids for investigating network formation and early transplantation events with hiPSC-derived neurons, providing flexibility to rapidly tune cell type(s), cell ratios, and traceable biomarkers. Copy rights belong to original authors. Visit the link for more info

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2020.09.04.282046v1?rss=1 Authors: Zuccoli, G. S., Nascimento, J. M., Codo, A. C., Moraes-Vieira, P. M., Rehen, S. S., Martins-de-Souza, D. Abstract: Schizophrenia is a severe psychiatric disorder of neurodevelopmental origin that affects around 1% of the world's population. Proteomic studies and other approaches have provided evidence of compromised cellular processes in the disorder, including mitochondrial function. Most of the studies so far have been conducted on postmortem brain tissue from patients and do not allow the evaluation of the neurodevelopmental aspect of the disorder. To circumvent that, we studied the mitochondrial and nuclear proteomes of neural stem cells (NSCs) and neurons derived from induced pluripotent stem cells (iPSCs) from schizophrenia patients versus healthy controls. Our results revealed differentially regulated proteins in pathways related to mitochondrial function, oxidative phosphorylation, cell cycle control, DNA repair, and neuritogenesis. Moreover, metabolic analysis of NSCs revealed alterations in mitochondrial function in schizophrenia-derived cells. Hence, this study shows that changes in important cellular processes are present during neurodevelopment and could be involved with the establishment of schizophrenia, as well as the phenotypic traits observed in adult patients. Copy rights belong to original authors. Visit the link for more info

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2020.08.12.247627v1?rss=1 Authors: Goepferich, M., George, N. O., Domingo Muelas, A., Bizyn, A., Pascual, R., Fijalkowska, D., Kalamakis, G., Müller, U., Krijgsveld, J., Mendez, R., Farinas, I., Huber, W., Anders, S., Martin-Villalba, A. Abstract: Autism spectrum disorder (ASD) is a neurodevelopmental disease affecting social behavior. Many of the high-confident ASD risk genes relate to mRNA translation. Specifically, many of these genes are involved in regulation of gene expression for subcellular compartmentalization of proteins. Cis-regulatory motifs that often localize to 3'- and 5'-untranslated regions (UTRs) offer an additional path for posttranscriptional control of gene expression. Alternative cleavage and polyadenylation (APA) affect 3'UTR length thereby influencing the presence or absence of regulatory elements. However, APA has not yet been addressed in the context of neurodevelopmental disorders. Here we used single cell 3'end sequencing to examine changes in 3'UTRs along the differentiation from neural stem cells (NSCs) to neuroblasts within the adult brain. We identified many APA events in genes involved in neurodevelopment, many of them being high confidence ASD risk genes. Further, analysis of 3'UTR lengths in single cells from ASD and healthy individuals detected longer 3'UTRs in ASD patients. Motif analysis of modulated 3'UTRs in the mouse adult neurogenic lineage and ASD-patients revealed enrichment of the cytoplasmic and polyadenylation element (CPE). This motif is bound by CPE binding protein 4 (CPEB4). In human and mouse data sets we observed co-regulation of CPEB4 and the CPEB-binding synaptic adhesion molecule amyloid beta precursor-like protein 1 (APLP1). We show that mice deficient in APLP1 show aberrant regulation of APA, decreased number of neural stem cells, and autistic-like traits. Our findings indicate that APA is used for control of gene expression along neuronal differentiation and is altered in ASD patients. Copy rights belong to original authors. Visit the link for more info

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2020.07.23.212423v1?rss=1 Authors: Badner, A., Reinhardt, E. K., Nguyen, T. V., Midani, N., Marshall, A. T., Lepe, C., Echeverria, K., Lepe, J., Torrecampo, V., Bertan, S. H., Tran, S. H., Anderson, A. J., Cummings, B. J. Abstract: Human neural stem cells (hNSCs) have potential as a cell therapy following traumatic brain injury (TBI). While various studies have demonstrated the efficacy of NSCs from on-going culture, there is a significant gap in our understanding of freshly thawed cells from cryobanked stocks - a more clinically-relevant source. To address these shortfalls, the therapeutic potential of our previously validated Shef-6.0 human embryonic stem cell (hESC)-derived hNSC line was tested following long-term cryostorage and thawing prior to transplant. Immunodeficient athymic nude rats received a moderate unilateral controlled cortical impact (CCI) injury. At 4-weeks post-injury, 6x105 freshly thawed hNSCs were transplanted into six injection sites (2 ipsi- and 4 contra-lateral) with 53.4% of cells surviving three months post-transplant. Interestingly, most hNSCs were engrafted in the meninges and the lining of lateral ventricles, associated with high CXCR4 expression and a chemotactic response to SDF1alpha (CXCL12). While some expressed markers of neuron, astrocyte, and oligodendrocyte lineages, the majority remained progenitors, identified through doublecortin expression (78.1%). Importantly, transplantation resulted in improved spatial learning and memory in Morris water maze navigation and reduced risk-taking behavior in an elevated plus maze. Investigating potential mechanisms of action, we identified an increase in ipsilateral host hippocampus cornu ammonis (CA) neuron survival, contralateral dentate gyrus (DG) volume and DG neural progenitor morphology as well as a reduction in neuroinflammation. Together, these findings validate the potential of hNSCs to restore function after TBI and demonstrate that long-term bio-banking of cells and thawing aliquots prior to use may be suitable for clinical deployment. Copy rights belong to original authors. Visit the link for more info

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2020.07.15.205021v1?rss=1 Authors: Dray, N., Mancini, L., Binshtok, U., Cheysson, F., Supatto, W., Mahou, P., Bedu, S., Ortica, S., Krecsmarik, M., Herbert, S., Masson, J.-B., Tinevez, J.-Y., Lang, G., Beaurepaire, E., Sprinzak, D., Bally-Cuif, L. Abstract: Neural stem cell (NSC) populations persist in the adult vertebrate brain over a life time, and their homeostasis is controlled at the population level. The nature and properties of these coordination mechanisms remain unknown. Here we combine dynamic imaging of entire NSC populations in their in vivo niche over weeks, pharmacological manipulations, mathematical modeling and spatial statistics, and demonstrate that NSCs use spatiotemporally resolved local feedbacks to coordinate their decision to divide. These involve a Notch-mediated inhibition from transient neural progenitors, and a dispersion effect from dividing NSCs themselves, exerted with a delay of 9-12 days. Simulations from a stochastic NSC lattice model capturing these interactions demonstrate that they are linked by lineage progression and control the spatiotemporal distribution of output neurons. These results highlight how local and temporally delayed interactions occurring between brain germinal cells generate self-propagating dynamics that maintain NSC population homeostasis with specific spatiotemporal correlations. Copy rights belong to original authors. Visit the link for more info

Weihnachtsgeschenke im April und Gäste im Jacuzzi. Diese Folge hat einfach alles. Georg Clowney, Bruce Chilles und Jessica Albern hatten keine Zeit, darum haben wir den viert-besten Gast eingeladen. Glücklicherweise ist dieser auch gekommen, sonst hätten wir schon alt ausgesehen. Ach so, Thema. Wir reden über NSCs, und im Speziellen über „Hochstatus-NSCs“. Vielen Dank an […]

Weihnachtsgeschenke im April und Gäste im Jacuzzi. Diese Folge hat einfach alles. Georg Clowney, Bruce Chilles und Jessica Albern hatten keine Zeit, darum haben wir den viert-besten Gast eingeladen. Glücklicherweise ist dieser auch gekommen, sonst hätten wir schon alt ausgesehen. Ach so, Thema. Wir reden über NSCs, und im Speziellen über „Hochstatus-NSCs“. Vielen Dank an […]

Nathanel Noiraud est le fondateur et CEO de N-strategy consulting services (NSCS), une société basée à Kuala Lumpur qui aide les start-ups et entreprises de la tech à se développer sur les marchés de l’Asie Pacifique. Nathanael se lance dans l’entrepreneuriat avec NSCS en 2016, après plusieurs expériences  internationales : en Amérique du Sud, aux Etats Unis, au Portugal et enfin en Asie pour des grands groupes dans le digital. NSCS compte aujourd’hui 60 collaborateurs, présents dans 19 pays. Avec Nathanael on a parlé de rêves d’explorations et de conquête de l’ouest. On a parlé du développement éclair de l’Asie, de comment le continent est passé du statut d’usine globale à celui d’un des marchés le plus attrayant du monde. On a aussi discuté du financement des start-ups, de la course aux levées de fond, et de l’impact que cela peut avoir sur l’entreprise. Pour suivre Nathanael et son actualité rendez-vous sur LinkedIn : https://www.linkedin.com/in/nathanael-noiraud-96b3b558/

Häufig haben sie nicht mal einen richtigen Namen, aber schlussendlich sind sie das das Salz in der Suppe: Die NSC, denen die Spieler auf ihrem Weg durch das Abenteuer begegnen, seien sie ihnen feindlich gesonnen oder hilfreiche Ratgeber. Wie man sie in Fate baut und verwendet, erklären André, Alex und Friederike euch in dieser Doppelfolge

Häufig haben sie nicht mal einen richtigen Namen, aber schlussendlich sind sie das das Salz in der Suppe: Die NSC, denen die Spieler auf ihrem Weg durch das Abenteuer begegnen, seien sie ihnen feindlich gesonnen oder hilfreiche Ratgeber. Wie man sie in Fate baut und verwendet, erklären André, Alex und Friederike euch in dieser Doppelfolge

Heute haben wir uns in voller Besetzung extra viel Zeit genommen, um uns einem sehr großen Thema zu widmen: Nichtspielercharaktere. Wir haben über ihre Funktion, Möglichkeiten und Darstellung gesprochen und waren uns nicht immer einig. Hört am besten einfach selbst und wir freuen uns über eure Meinung!

The Pit Stop hosted by Tim Disspain and Steven Wilson live from Talladega, AL every Tuesday night at 7/Eastern 6/Central covering the NSCS,NXS,NCWTS,ARCA,NK&N Series and local dirt track racing from Talladega Short Track.We also give you the latest penalties and fines for crew members and teams issued by Nascar. We are the only live Nascar talk show in the state of Alabama our guest range from drivers,spotters, team owners to track presidents and Nascar officals.Tune in and call (215) 383-3681.

The Pit Stop hosted by Tim Disspain and Steven Wilson live from Talladega, AL every Tuesday night at 7/Eastern 6/Central covering the NSCS,NXS,NCWTS,ARCA,NK&N Series and local dirt track racing from Talladega Short Track.We also give you the latest penalties and fines for crew members and teams issued by Nascar. We are the only live Nascar talk show in the state of Alabama our guest range from drivers,spotters, team owners to track presidents and Nascar officals.Tune in and call (215) 383-3681.

The Pit Stop hosted by Tim Disspain and Steven Wilson live from Talladega, AL every Tuesday night at 7/Eastern 6/Central covering the NSCS,NXS,NCWTS,ARCA,NK&N Series and local dirt track racing from Talladega Short Track.We also give you the latest penalties and fines for crew members and teams issued by Nascar. We are the only live Nascar talk show in the state of Alabama our guest range from drivers,spotters, team owners to track presidents and Nascar officals.Tune in and call (215) 383-3681.

The Pit Stop hosted by Tim Disspain and Steven Wilson live from Talladega, AL every Tuesday night at 7/Eastern 6/Central covering the NSCS,NXS,NCWTS,ARCA,NK&N Series and local dirt track racing from Talladega Short Track.We also give you the latest penalties and fines for crew members and teams issued by Nascar. We are the only live Nascar talk show in the state of Alabama our guest range from drivers,spotters, team owners to track presidents and Nascar officals.Tune in and call (215) 383-3681.

The Pit Stop hosted by Tim Disspain and Steven Wilson live from Talladega, AL every Tuesday night at 7/Eastern 6/Central covering the NSCS,NXS,NCWTS,ARCA,NK&N Series and local dirt track racing from Talladega Short Track.We also give you the latest penalties and fines for crew members and teams issued by Nascar. We are the only live Nascar talk show in the state of Alabama our guest range from drivers,spotters, team owners to track presidents and Nascar officals.Tune in and call (215) 383-3681.

The Pit Stop hosted by Tim Disspain and Steven Wilson live from Talladega, AL every Tuesday night at 7/Eastern 6/Central covering the NSCS,NXS,NCWTS,ARCA,NK&N Series and local dirt track racing from Talladega Short Track.We also give you the latest penalties and fines for crew members and teams issued by Nascar. We are the only live Nascar talk show in the state of Alabama our guest range from drivers,spotters, team owners to track presidents and Nascar officals.Tune in and call (215) 383-3681.

Heath White and Tyler Overstreet talk about Daytona, the Oyster Pub, The JRM 2016 Tour and who their picks are for this weekend's NCWTS, NXS and NSCS races. Learn more about your ad choices. Visit podcastchoices.com/adchoices

Monday means another edition of Mr C.J. Sports and Steve "Big Sweat" Billmyer talking about the past weekend's NASCAR activities on "Rubbin Is Racin" produced by The 110 Nation Sports Radio Network. We'll discuss the NSCS and NXS racing at Charlotte Motor Speedway and what we expect to happen at Kansas Speedway this coming weekend. So grab a "frosty beverage', kick back, and listen in. We'll make it worth your while.

Wenn wir wöchentlich sagen, dann meinen wir wöchentlich: Hier ist die neue Podcast-Folge. Unsere Themen: Dungeons and Dragons Classics: D&D-Material ist jetzt wieder als PDF käuflich zu erwerben Lebendige NSCs erschaffen und verkörpern Polyeder Podcast, Folge 19: D&D Classics mit … Weiterlesen →

Studies have suggested that there is a strong correlation between the masses of nuclear star clusters (NSCs) and their host galaxies, a correlation which is said to be an extension of the well-known correlations between supermassive black holes (SMBHs) and their host galaxies. But careful analysis of disk galaxies-including 2D bulge/disk/bar decompositions-shows that while SMBHs correlate with the stellar mass of the bulge component of galaxies, the masses of NSCs correlate much better with the total galaxy stellar mass. In addition, the mass ratio M-NSC/M-star,M- tot for NSCs in spirals (at least those with Hubble types Sc and later) is typically an order of magnitude smaller than the mass ratio M-BH/M-star,M- bul of SMBHs. The absence of a universal ``central massive object'' correlation argues against common formation and growth mechanisms for both SMBHs and NSCs. We also discuss evidence for a break in the NSC-host galaxy correlation, galaxies with Hubble types earlier than Sbc appear to host systematically more massive NSCs than do types Sc and later.

Heute gehe ich auf ein kleines Konzept namens "Conservation of NPCs" ein, dass für Spieler wie Spielleiter aller Spielstile hilfreich sein kann und eigentlich nur eine kleine Erinnerung ist, nicht für jede Rolle einen neuen Charakter ins Spiel einzuführen. Tja-ja, so ist das manchmal mit der Pünktlichkeit. Dafür ist allerdings die Seite AnalogSpieler.de endlich "fertig gestellt" und voll betriebsbereit. Schaut doch mal vorbei, falls Ihr Euch für Podcasts zum Thema Spiele (und mehr!) interessiert. Vielen Dank fürs Zuhören und bis zum nächsten Mal! 7:46 minutes (5.35 MB)

Debates of the latest NASCAR headlines, previews and predictions for NNS and NSCS in Atlanta and NCWTS in Kentucky, race weekend weather forecast with Brian Neudorff, and Jimmy Joe's Atlanta NASCAR update.

Debates of the latest NASCAR headlines, previews and predictions for NNS and NSCS in Atlanta and NCWTS in Kentucky, race weekend weather forecast with Brian Neudorff, and Jimmy Joe's Atlanta NASCAR update.

Join us as Nate Ryan from USA Today stops by to help discuss and debate the latest NASCAR hot topics. We will also get you revved up for NSCS and NNS in Michigan and NCWTS in Darlington!

Join us as Nate Ryan from USA Today stops by to help discuss and debate the latest NASCAR hot topics. We will also get you revved up for NSCS and NNS in Michigan and NCWTS in Darlington!

Join us as we discuss and debate the latest NASCAR hot topics. We will also get you revved up for NSCS and NNS in Watkins Glen and NCWTS Nashville! Guest TBA.

Join us as we discuss and debate the latest NASCAR hot topics. We will also get you revved up for NSCS and NNS in Watkins Glen and NCWTS Nashville! Guest TBA.

NASCAR Sprint Cup Series champion Jimmie Johnson and crew chief Chad Knaus talk about celebrating their third consecutive title in New York City.

Erinnert ihr euch an die guten alten Zeiten, als Abenteuer unter der Erde stattfanden, NSCs zum Töten da waren und das Finale eines jeden Verlieses der Drache in der 20. Verliesebene war? Diese Zeiten sind zurück. Dungeon Crawl Classics verschwenden eure Zeit nicht mit langweiligen Reden, seltsamen Kampagnenhintergründen oder NSCs, die nicht getötet werden sollen. […]