Podcasts about korber

On episode 744 of WHAT THE TRUCK?!? Dooner is talking about an explosion in Ningbo aboard Yang Ming's YM Mobility. Are lithium ion batteries to blame again?  Korber's Rik Schrader talks about their big deal for MercuryGate.  WeatherOptics' Scott Pecoriello looks at fallout from Hurricane Debby and looks ahead to how the rest of the storm season could impact freight.  Verified Carrier's Andrey Drotenko talks about the $700 million-a-year cargo theft problem this industry faces and how Verified Carrier is fighting back. Plus, June sets import record; freight recession has drivers taking on strange side hustles; trucking in the ‘70s; and more.  Watch on YouTube Check out the WTT merch store Visit our sponsor Subscribe to the WTT newsletter Apple Podcasts Spotify More FreightWaves Podcasts Learn more about your ad choices. Visit megaphone.fm/adchoices

On episode 744 of WHAT THE TRUCK?!? Dooner is talking about an explosion in Ningbo aboard Yang Ming's YM Mobility. Are lithium ion batteries to blame again?  Korber's Rik Schrader talks about their big deal for MercuryGate.  WeatherOptics' Scott Pecoriello looks at fallout from Hurricane Debby and looks ahead to how the rest of the storm season could impact freight.  Verified Carrier's Andrey Drotenko talks about the $700 million-a-year cargo theft problem this industry faces and how Verified Carrier is fighting back. Plus, June sets import record; freight recession has drivers taking on strange side hustles; trucking in the ‘70s; and more.  Watch on YouTube Check out the WTT merch store Visit our sponsor Subscribe to the WTT newsletter Apple Podcasts Spotify More FreightWaves Podcasts Learn more about your ad choices. Visit megaphone.fm/adchoices

On Episode 743 of WHAT THE TRUCK?!?, Dooner is talking about August's red-hot FreightTech M&A. This week, Korber acquired MercuryGate and Bestpass grabbed Drivewyze. What's driving the deals, and what company will be scooped up next? Leonard's Express is teaming up with Optimal Dynamics to enhance driver experience and asset utilization.  We'll find out from Mike McGovern how they're using tech and AI to reshape their transportation network. Engine Technology Forum's Allan Schaeffer is fresh off a trip to Capitol Hill to attend the EESI Energy Efficiency and Renewable Energy Expo. We'll find out the latest on the push for zero-emissions solutions and learn how they're being deployed in the real world. As powerful as tech is, this is still a people industry. IamFr8's Tristan Bordallo is all about community-driven transportation. Bordallo is on a mission to educate the industry – shippers, receivers, brokers, 3PLs and customers – that drivers are human beings and their time and service are valuable. Plus, Aurora raises $483 million; cats and dogs rate the strap work and more. Watch on YouTube Check out the WTT merch store Visit our sponsor Subscribe to the WTT newsletter Apple Podcasts Spotify More FreightWaves Podcasts Learn more about your ad choices. Visit megaphone.fm/adchoices

On Episode 743 of WHAT THE TRUCK?!?, Dooner is talking about August's red-hot FreightTech M&A. This week, Korber acquired MercuryGate and Bestpass grabbed Drivewyze. What's driving the deals, and what company will be scooped up next? Leonard's Express is teaming up with Optimal Dynamics to enhance driver experience and asset utilization.  We'll find out from Mike McGovern how they're using tech and AI to reshape their transportation network. Engine Technology Forum's Allan Schaeffer is fresh off a trip to Capitol Hill to attend the EESI Energy Efficiency and Renewable Energy Expo. We'll find out the latest on the push for zero-emissions solutions and learn how they're being deployed in the real world. As powerful as tech is, this is still a people industry. IamFr8's Tristan Bordallo is all about community-driven transportation. Bordallo is on a mission to educate the industry – shippers, receivers, brokers, 3PLs and customers – that drivers are human beings and their time and service are valuable. Plus, Aurora raises $483 million; cats and dogs rate the strap work and more. Watch on YouTube Check out the WTT merch store Visit our sponsor Subscribe to the WTT newsletter Apple Podcasts Spotify More FreightWaves Podcasts Learn more about your ad choices. Visit megaphone.fm/adchoices

WMS and supply chain systems come in various shapes and sizes. And there are very few systems in the market that could catch up with the speed of the cloud ERP vendors. While NetSuite and Acumatica both had native capabilities for WMS, they both relied on High Jump for advanced WMS capabilities. While High Jump succeeded in being a default choice for a WMS solution, the game has been completely changed since its acquisition by Korber. And now Korber can provide similar capabilities as Manhattan or Blue Yonder. Korber also has some unique layers, such as managing DSD and last mile, as well as transportation spend management. So where do High Jump and Korber stand in their capabilities?In today's episode, we invited a panel of industry experts for a live discussion on LinkedIn to conduct an independent review of Korber/HighJump capabilities. We covered many grounds, including their unique strength in the cloud native market, and how they rule the mid-market, especially in the NetSuite and Acumatica ecosystems. We also covered their other strengths, such as being comparable to larger solutions such as Blue Yonder or Manhattan in the large category space and having unique capabilities such as DSD or freight spend management.For more information on growth strategies for SMBs using ERP and digital transformation, visit our community at wbs. rocks or elevatiq.com. To ensure that you never miss an episode of the WBS podcast, subscribe on your favorite podcasting platform. 

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613Most parents are struggling on keeping their kids engaged. They tend to keep on looking for ways to keep their children's minds ready to learn more. In this episode, our guest will introduce a great tool to help parents with this kind of dilemma. Discover a great tool to use to start communicating with your kids.In today's 3-21 NoKiddin' episode, I was joined by Ken Korber. He is an author, clinician, and founder of the Center for Functional Learning. Ken left his full-time bedside care to focus on patient education and health promotion as a children's book storyteller and founder of the Center for Functional Learning.Ken was able to publish writings that have led to education and health promotion grant awards. He uses the Musical Adventure™ characters to build reading skills, foster an appreciation for music, and advocate for the wellness of the general public, especially within at-risk/vulnerable communities of children and adults.Join me as he discussed Grace and her musical adventure. And everything that led him to create Grace and her friends.Connect with Ken:Website: https://centerforfunctionallearning.com/Instagram: https://www.instagram.com/centerforfunctionallearning/Happy Friday!------------------------------------------------Did you enjoy the show? Please leave us a rate and review!This helps us reach other gamblers who may need to hear recovery messages.------------------------------------------------Get in touch with me!Email address : bobbie@321NoKiddin.comFacebook          : www.facebook.com/321NoKiddinInstagram         : www.instagram.com/bobbietheawesomestPinterest           : www.pinterest.ph/bobbiemalatestaLinkedIn            : www.linkedin.com/in/bobbiemalatestaYouTube           : www.youtube.com/channel/UCkUV58i4z2Se3jXuDldcXaAWebsite            : www.321nokiddin.com**A super special thanks to Justin Furstenfeld for granting us permission to use his music on the show!Please support and follow the Blue October band on:Facebook  : www.facebook.com/blueoctoberInstagram : www.instagram.com/blueoctoberbandTwitter       : www.twitter.com/blueoctoberYoutube    : www.youtube.com/user/blueoctoberofficialSupport the show9 of us wrote about the concept of Belonging and share our individual stories about how we found belonging.Please check it out in the link below!Link to our book- BELONGING- Secrets to Soothe the Soul https://amzn.to/45qS6pl Get in touch with me! Questions or feedback or just want to connect? Email address : bobbie@321NoKiddin.com Facebook : www.facebook.com/321NoKiddin Instagram : www.instagram.com/bobbietheawesomest Pinterest : www.pinterest.ph/bobbiemalatesta LinkedIn : www.linkedin.com/in/bobbiemalatesta YouTube : www.youtube.com/channel/UCkUV58i4z2Se3jXuDldcXaA Website : www.321nokiddin.com **A super special thanks to Justin Furstenfeld for granting us permission to use his music on the show!Please support and follow the Blue October band on: Facebook : www.facebook.com/blueoctober Instagram : www.instagram.com/blueoctoberband Twitter : www.twitter.com/blueoctober Youtube : www.youtube.com/user/blueoctoberofficial Support the show

613Most parents are struggling on keeping their kids engaged. They tend to keep on looking for ways to keep their children's minds ready to learn more. In this episode, our guest will introduce a great tool to help parents with this kind of dilemma. Discover a great tool to use to start communicating with your kids.In today's 3-21 NoKiddin' episode, I was joined by Ken Korber. He is an author, clinician, and founder of the Center for Functional Learning. Ken left his full-time bedside care to focus on patient education and health promotion as a children's book storyteller and founder of the Center for Functional Learning.Ken was able to publish writings that have led to education and health promotion grant awards. He uses the Musical Adventure™ characters to build reading skills, foster an appreciation for music, and advocate for the wellness of the general public, especially within at-risk/vulnerable communities of children and adults.Join me as he discussed Grace and her musical adventure. And everything that led him to create Grace and her friends.Connect with Ken:Website: https://centerforfunctionallearning.com/Instagram: https://www.instagram.com/centerforfunctionallearning/Happy Friday!------------------------------------------------Did you enjoy the show? Please leave us a rate and review!This helps us reach other gamblers who may need to hear recovery messages.------------------------------------------------Get in touch with me!Email address : bobbie@321NoKiddin.comFacebook          : www.facebook.com/321NoKiddinInstagram         : www.instagram.com/bobbietheawesomestPinterest           : www.pinterest.ph/bobbiemalatestaLinkedIn            : www.linkedin.com/in/bobbiemalatestaYouTube           : www.youtube.com/channel/UCkUV58i4z2Se3jXuDldcXaAWebsite            : www.321nokiddin.com**A super special thanks to Justin Furstenfeld for granting us permission to use his music on the show!Please support and follow the Blue October band on:Facebook  : www.facebook.com/blueoctoberInstagram : www.instagram.com/blueoctoberbandTwitter       : www.twitter.com/blueoctoberYoutube    : www.youtube.com/user/blueoctoberofficialSupport the show

Today, we interview author Ken Korber and how he was able to accelerate his student loan repayment plan by writing several children's books. The best part is that you can do it too!  Ken provides a deep dive into the process of writing your first book so that you can direct your profits to your student loans. Ken is a surgical and cardiovascular physician assistant turned author who left bedside care to focus on patient education and health promotion, as a children's book author. Visit the show notes for all the extra details: https://thestudentloanpodcast.com/episode77 Visit our podcast sponsor at StartNoo.com to see if StartNoo is on your campus and learn how you can exchange service hours in the community for direct payments towards your student loans or tuition.

Kenneth Korber is trained as a surgical and cardiovascular physician assistant (PA).  He has since become an author and entrepreneur. He left full-time bedside care to focus on patient education and health promotion as a children's book storyteller and founder of the Center for Functional Learning.Kenneth's published writings have led to education and health promotion grant awards. He uses the Musical Adventure™ characters to build reading skills, foster an appreciation for music, and advocate for wellness of the general public, especially within at-risk/vulnerable communities of children and adults.To learn more about Kenneth and his organization visit www.centerforfunctionallearning.com Support the show

Ali is a current MSW student at Florida State University. She earned her B.S. degree from the University of Central Florida, where she majored in Clinical Psychology. Ali has worked with many different populations in the human services field. While obtaining her undergraduate degree, she interned with multiple homeless shelters in Pinellas County, where she led many milieu therapy groups, supervised a group of interns, and helped locate various resources for the homeless residents of Pinellas and Pasco counties. During the first year of her graduate education, she served as a graduate assistant for the Unconquered Scholars Program at The Center for Academic Retention and Enhancement. She worked with youth who have experienced homelessness, foster care, relative care, and ward of the state status. In her free time, she volunteers for the National Alliance on Mental Illness, where she implemented and continues to lead a support group centered around the struggles of young adults. Ali is passionate about advocating for others' mental health and bettering the lives of people in her community. --- Support this podcast: https://anchor.fm/normalizetheconversation/support

Kenneth Korber & Mylo Schaaf

Kenneth Korber & Mylo Schaaf

Prav v teh dneh po domovih marsikje že zaganjajo peči in odpirajo radiatorje, ob tem pa se številnih polašča strah pred previsokimi stroški. Energetski strokovnjaki pritrjujejo, da je pred nami turbulentna jesen, razmere so kaotične in napovedi, kako bo čez mesec ali dva, so negotove. A kljub temu lahko preverimo, po kakšnih osnovnih ukrepih lahko posežemo doma, da bomo energetsko varčnejši, in kaj pomenijo vladni ukrepi za zmanjševanje energetske draginje: kdo si lahko obeta najdražje položnice in kdo celo prihranke? Pozabili nismo niti na okoljevarstveno vprašanje: zaradi varčevanja bo v peči romalo marsikaj, kar utegne močno onesnažiti zrak. Članek Premešane karte cen energentov je na voljo na spletni strani Moje finance.  Sogovorniki:  Srečko Korber iz revije Moje finance;  dr. Tomislav Tkalec, Fokus - društvo za sonaravni razvoj;  dr. Griša Močnik z Univerze v Novi Gorici in Inštituta Jožefa Stefana, ki se strokovno med drugim posveča bremenu črnega ogljika v zraku;  Bojan Žnidaršič, neodvisni energetski svetovalec.  V oddaji smo predvsem potipali teren pred prihajajočo kurilno sezono, ki prinaša negotovost zaradi višanja cen energentov. Področje bomo z različnih vidikov spremljali še naprej, vabljeni, da svoja vprašanja in premisleke delite z nami na elektronskem naslovu val202@rtvslo.si. uredništvo Vala 202

Christian Wölbeling is an experienced Senior Director with Korber Pharma. He was nominated as a “Pharma Industry Leader” by The International Society for Pharmaceutical Engineering (ISPE) Pharmaceutical Engineering Magazine in 2020, and has been a keynote speaker at many international pharmaceutical engineering conferences. He has a demonstrated history of working for the pharmaceutical industry with over 30 years in the field of Manufacturing IT. As a leader with ISPE, Christian is also focused on supporting the industry with new thinking in the direction of the ISPE Pharma 4.0™ Operating Model. The Industry 4.0 Podcast with Grantek delivers a look into the world of manufacturing, with a focus on stories and trends that lead to better solutions. Our guests will share tips and outcomes that will help improve your productivity. You will hear from leading providers of Industrial Control System hardware and software, Grantek experts and leaders at best-in-class industry associations that serve Life Sciences and Food & Beverage manufactures.

Danis Arbeiten drehen sich um Theater, Musik und Soziales. Er folgt dabei seiner Neugier und heisst Herausforderungen herzlich willkommen. Mal arbeitet er alleine, mal in grossen Produktionen mit Menschen aus allen Ecken der Gesellschaft, mal für bestehende Formationen, mal reisst er selber etwas an.Dani will überraschen, andere und sich selbst.Und er ist Schweizerischer Vizemeister 2014 im Powerpointkaraoke. Das kann nicht jede:r von sich behaupten.Dani hat den Master of Arts in Theater an der ZHdK abgeschlossen, verschiedenste theaterpädagogische Projekte geleitet, tritt selber auf (als Musiker oder Schauspieler), inszeniert Theater und Musicals (wie z.B. "Verona 3000" oder aktuell das "Sturzballett") und wagt sich seit neustem auch als Filmemacher in neue Gefilde. Mehr Informationen zu Dani findest du unter: https://www.danielkorber.com

As Ran Korber, CEO and Founder of BreezoMeter, was trying to find a place to settle and raise his family, he wanted to make sure he was doing so in an area that had minimal air pollution. However, he soon found that there was no solution that catered to his need of localized air and climate data. An experienced environmental engineer, Ran set out to build a system that could provide targeted information about climate conditions in various locales. A few investments, UN recognition, and now a 400 million person user-base later, Ran is redefining climate tech with BreezoMeter and believes that environmental data, aka intelligence, is the way forward in truly understanding how to combat the effects of climate change. Jordan Kastrinsky - https://www.linkedin.com/in/jordan-kastrinsky-9b8b2370/ Ran Korber - https://www.linkedin.com/in/ran-korber-aa721a29/ Join our community: Front Team website - https://www.front-team.com Our telegram channel - https://t.me/front_team

Mercado & Perspectivas, uma iniciativa da FecomercioSP, debate as boas práticas, soluções para retomada e iniciativas inovadoras das empresas brasileiras.Neste episódio Marcelo Korber, head de Relação com Investidores do Grupo Mateus, trata das estratégias de logística e expansão da empresa, a aceleração multicanal experimentada durante a pandemia e as expectativas para 2022.Acesse: www.fecomercio.com.br │ Conheça: http://lab.fecomercio.com.br/©️ FecomercioSP 2022. Todos os direitos reservados.

In this full-on, bold interview, Michael Stietz, SVP and Chief Procurement Officer of Korber, sheds light on how the procurement function has changed over the past few decades and since the Covid-19 pandemic ensued in March of 2020. Michael shares with us his advice on navigating the trade-off between cost reduction, quality, and supplier-led innovation for competitive advantage and gives us a rundown of the next chapter for procurement and his golden rules for digitization.   About The Speaker Michael Stietz is Senior Vice President & Chief Procurement Officer of Körber AG in Hamburg, Germany. Michael is responsible for the management and development of the Group Procurement and Supply Chain Management function. For more than 15 years, he has been working in various Operations, Procurement, SCM functions. He is the initiator and co-founder of the first Procurement Chair at the University of Mannheim, Germany, and a guest lecturer at the Universities of Mannheim, Berlin, and Karlsruhe.

Ran is the CEO & Co-founder at BreezoMeter. His burning ambition is to improve the health and quality of life for billions of people across the globe by providing accurate and actionable air quality data. As co-founder and CEO at BreezoMeter, he is proud to be able to work towards bringing  dream to fruition each day.

durée : 01:30:01 - Avec Serge Korber, réalisateur - par : Benoît Duteurtre - "Serge Korber cinéaste connu pour "L’Homme-orchestre" avec Louis de Funès, "Un idiot à Paris" avec Jean Lefebvre, l'ami de Ricet Barrier, Boby Lapointe à leurs débuts au Cheval d'Or, l'assistant de Guy Debord, le copain de Jean-Louis Trintignant, le complice d'Alain Goraguer..." Benoît Duteurtre - réalisé par : Christine Amado

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2020.11.03.366690v1?rss=1 Authors: Oberbeckmann, E., Krietenstein, N., Niebauer, V., Wang, Y., Schall, K., Moldt, M., Straub, T., Rohs, R., Hopfner, K.-P., Korber, P., Eustermann, S. Abstract: The fundamental molecular determinants by which ATP-dependent chromatin remodelers organize nucleosomes across eukaryotic genomes remain largely elusive. Here, chromatin reconstitutions on physiological, whole-genome templates reveal how remodelers read and translate genomic information into nucleosome positions. Using the yeast genome and the multi-subunit INO80 remodeler as a paradigm, we identify DNA shape/mechanics encoded signature motifs as sufficient for nucleosome positioning and distinct from known DNA sequence preferences of histones. INO80 processes such information through an allosteric interplay between its core- and Arp8-modules that probes mechanical properties of nucleosomal and linker DNA. At promoters, INO80 integrates this readout of DNA shape/mechanics with a readout of co-evolved sequence motifs via interaction with general regulatory factors bound to these motifs. Our findings establish a molecular mechanism for robust and yet adjustable +1 nucleosome positioning and, more generally, remodelers as information processing hubs that enable active organization and allosteric regulation of the first level of chromatin. Copy rights belong to original authors. Visit the link for more info

Mit seinem Bühnen Programm Brot & Rosen, tritt Daniel Korber am 29. Oktober 2020 im Kleintheater auf. Zu diesem Anlass hat uns Daniel Korber, in der Sendung, zwei Songs gespielt.

Ran Korber is Co-Founder and CEO at BreezoMeter, the leading air quality analytics company. It is one of Israel's top startups and has almost 100 million daily users from its customers, including Dyson, L'Oreal, Bosch, Unilever, and many more. In this episode… Good air quality is so critical to life but oftentimes, we take it for granted. Back in 2013 when Ran Korber and his wife were planning on buying a house, they decided that one of their main considerations was having it in an area with the best air quality. To their surprise, there was no reliable data and no accurate way to measure the air quality anywhere. Because of this realization and his desire to protect his family’s health, Ran and his friends, Emil Fisher and Ziv Lautman, decided to create a product that will help address this problem. They developed very sophisticated algorithms capable of forecasting and monitoring air pollution globally. The result of that project is BreezoMeter. They are now a leading air quality analytics company that offers services worldwide with accurate data and actionable recommendations. On this episode of Inspired Insider as Dr. Jeremy Weisz talks with Ran Korber of BreezoMeter about how their brand helps people to protect their health. Ran also shares how he survived COVID-19, the personal story behind BreezoMeter, how he convinced a team of experts to work with their company, and why 2020 is his best and most challenging year yet. Stay tuned.

Welcome back to episode 425 of the Whole View. (0:27)   In previous episodes, Sarah and Stacy have talked a lot about the progression timeline and what is happening in the state of the world.   For context, Stacy tells the audience that at the time of this recording it is October 7th, 2020.   She feels that it is important to know due to the ever-changing atmosphere around the Covid-19 pandemic.   This episode has interesting context for Stacy. Her family (specifically she and her husband) had Covid-19 in late April/early May of 2020.   Sarah and Stacy did a show covering Stacy's experiences with Covid-19, as well as a blog post going through the timelineof her experience.   She does not plan to revisit that in this episode. However, she believes it to be relevant to some of the long-term side effects the Coronavirus can have.   A Message On Patreon We are always looking for ways to spread positivity in 2020. Before we dive into this episode, we'd like to share with you a wonderful message we received.   This message comes from Teri, who is one of our patrons!   In case some of our listeners don't know, you can sign up and support The Whole View by becoming a member on Patreon.   We provide bonus audio on a weekly basis and behind the scenes looks at our unfiltered thoughts for each episode.   Here's what Teri wrote: Long-time listener here, and I adore the Patreon content! I wondered if it would be short content, but "knowing" you, why would I have thought that?! You give so much, and the Patreon episodes are no different. I utterly loved the first two episodes, and really enjoyed hearing about your move from identifying as Paleo and the changes in the Paleo community, especially around racial justice and anti-science.  I'm a Whole30 coach and share your articles and podcasts with my clients so frequently. I also help moderate a Whole30 FB group of about 4,000 over-50 (mostly) women and when it's my turn to moderate I frequently have Science Week - and guess who is featured prominently each time? I adore the science, the friendly chat, the laughs and the tips. Truly, this new Patreon content is a bright gift in this very dark time. Thank you both! Thank you, Teri! For our listeners, if you would like to join our Patreon family, you can find us at patreon.com/thewholeview for bonus and unfiltered content. Stacy notes that Sarah has moved away from swearing in general and uses some very interesting alternative terms. So if you're curious and want to be creative, you know where to go!   A Quick Recap Today Sarah and Stacy will be focusing on the long-tail symptoms after Covid infection, the reinfection stories that have been circulating, "long-haulers," and the potential long-term effects. (4:42)   Here is a quick recap of our previous Covid-19 episodes and where you can find them:   Episode 396: this was early on, so Sarah and Stacy talked about known (at the time) epidemiology and why it was important to take the Coronavirus shut down seriously.    They also talked about autoimmune diseases and Covid-19, antibacterial soaps versus natural soaps, and the health effects of using disinfectants.   They discussed the evidence efficacy (or rather lack-there-of) of silver-containing solutions. And did a bit of myth-busting surrounding elderberry for autoimmune disease.    Finally, Stacy and Sarah talked about taking Vitamin C, Vitamin D, and zinc.   In Episode 401, Stacy and Sarah explored the way out of this pandemic and how to get back to normal life as we know it.They touched a little bit on reinfection cases, which they will be taking at a more in-depth look at today. They also broke down the science behind wearing face masks.  In Episode 412, Stacy and Sarah talked about how isolation can impact immune function. They also myth-busted some of the concerns that have circulated around it. They discussed how isolation might affect the gut microbiome, public health messaging, and the huge importance of vitamin D when reducing the risk of Covid-19.  In today's episode, Sarah reminds us that they will be focusing on: Reinfection versus Reactivation Long haulers (sometimes "Long Covid" Longterm complications/damage   Why This Matters Stacy shares that this has been an ongoing thing for her. (7:50) Stacy has shared in past podcasts that she had a feeling there were two unknowns that we would find out later on about the Coronavirus: First, there's an effect on the brain, which has since been shown. And second, that there is a hormonal impact on the body. Stacy has noticed she gets reactivated symptoms during her menstrual cycles, specifically inflammation. Stacy feels that it is really important to talk about because there's so much focus on how many people have died. As someone who's gone through it and now living with the long-term side effects of it, she feels it's important to acknowledge that millions of people in the world survived but are still feeling the effects months later. She considers herself one of the lucky ones since she had a mild case and cannot be more thankful for that. Stacy would like the audience to understand that Covid-19 is not like the flu. You don't get it, and then you're done, and you have immunity for a short time until the next strain comes out.   Answers Through Science And she thinks that Sarah going through the science of it will be very beneficial to people. Sarah points out that we're now at a point where more research and studies are trying to look at the spectrum of the long term impacts. It's really important to understand the long-term impact of this so that we can monitor at-risk people for potential health-specific complications in the future. Sarah also references potential economic impacts we could see due to prolonged symptoms and brain fog keeping millions of people from returning to work. There are down-stream effects that we need to really understand and be able to wrap our heads around. Sarah also explains that there's a lot of work that still needs to be done to reduce the spread. She also explains that we are still at a really high level of new cases every day. With high community spread, it makes any kind of gathering (school, church, weddings, funerals) all very challenging. As we're trying to figure out how to continue with precautions to reduce spread, Sarah expresses the need to understand this whole other side of the virus that's not related to the two to three weeks of active infection.   ADE And How It Works Sarah first wants to look into whether the recent reports that look like reinfection are truly that. (14:26) Stacy shares that this is something especially concerning to her. Sarah explains antibody-dependent enhancement of infection (ADE or ADI). It is a common phenomenon in many other viruses, such as influenza A virus, Coxsackievirus B, respiratory syncytial virus (RSV), Ebola virus, human immunodeficiency virus (HIV) dengue virus. Dengue Fever is the best understood in terms of the ADE mechanism. There are four known strains of the virus; when you contract one strain, you get Dengue Fever, and your body mounts a response. You are then protected for a relatively short time frame of time, not just against the strain you caught but also the other three. As the antibodies fall in number, there's a window where if you get exposed to one of the other three strains, you can develop a much more severe case. If you don't get exposed to one of those three, you're still immune to the first strain you caught even after all the antibodies are gone. If you catch one of the other three strains after the window is closed, you will develop regular Dengue Fever again, but not get the life-threatening reaction. Sarah explains that that magic sweet spot with a low level of antibodies actually magnifies the infection. One thing Sarah notes is that the Dengue Fever virus actually replicates inside immune cells. So when there are high levels of antibodies shortly after an infection, these "eater cells" are able to neutralize the infection. When there are low levels of antibodies, they're not sufficient enough to neutralize the virus. This sweet spot typically starts from a few months out from infection and can last to about a year.   What This Means For Covid-19 Sarah states Covid-19 does not appear to be very good at infecting and replicating inside immune cells.   Because of that very important difference, there's no evidence of ADE in Covid-19 (SARS-CoV-2).   SARS-CoV, which caused the SARS outbreak in 2002 and 2003, is the most closely related coronavirus causing Covid-19.   Sarah explains that from what we know in early vaccine development for SARS-CoV, there was what's called vaccine-enhanced infection.   This takes place due to a similar mechanism as ADE.   Because of that, Sarah explains we can't say for sure whether or not we will see this occur in Covid-19.   Reinfections We only have a few dozen confirmed cases in the medical literature of what looks like reinfection with Covid-19. (20:10)   Sarah reminds us that the pandemic is not even a year old yet.   She explains that there are seven different coronaviruses that infect humans.   Several are responsible for about a quarter to a third of common cold cases annually.   Sarah points out that research on these common cold coronaviruses shows immunity doesn't last that long- maybe as little as a year.   Sarah outlines several studies that measured infection rates and discusses the results in terms of developing immunity.   She references this article for more information.   Sarah explains that many test subjects showed partial immunity. This means their bodies had enough familiarity with the virus to fight it with less symptoms.   However, it is important to note that during that time, you are still very infectious.   Sarah explains that having partial immunity to Covid-19 could potentially result in more asymptomatic reinfections.   This would prove to be incredibly difficult to manage from a containment point of view.   Covid-19 Spread About 40% of spread happening right now is from a combination of asymptomatic and pre-symptomatic people.   This means that even if you're immune enough that you might not get a symptomatic infection upon a subsequentexposure, you may have a higher likelihood of being an asymptomatic carrier.   Sarah tells us that SARS from the 2002 and 2003 epidemic is about 80% the same as the Covid-19 virus in terms ofgnome sequence.   It's comparable to 50% to MERS, and less than that to common cold coronaviruses.   Immunity to SARS seemed to last typically 2 to 3 years (but up to 6), as measured by memory T cells.    Sarah references several more studies that explore results of immunity in different test groups.   Articles with more scientific results and information can be found here, here, and here.   Sarah explains that all this data tells us that reinfection is something that we may need to be concerned about.   But there's not mechanistically a good argument for thinking we're going to see it this early.   Covid-19 Reactivation What's happened in these few dozen cases of possible Covid-19 reinfection is that people tested positive and were sent home. They recovered and later tested negative. Then, months later, they once more test positive for Covid-19. Some are similarly sick as the first time, some are sicker the second time, and others are asymptomatic. This begs the question if it's real reinfection. Sarah claims that of the researchers studying these cases, nobody is convinced that it's true reinfection. None of these cases are from immunocompromised people and aren't thought to be very concerning. Reinfection is a legitimate concern for those with autoimmune disorders, such as HIV. A more likely explanation for what is going on with these cases is that it's actually viral reactivation. This means that these people are long-haulers who had a latent period, combined with testing limitations and failures. It's possible that those negative tests weren't true negatives. Sarah explains this as the immune system almost beat it, but didn't neutralize it fully, and pulled back fighting too soon. There's a really wide range in how long people have measurable virus in their systems, and they may not be infectious the entire time. Sarah explains that there's a lot that we still don't know about the Covid-19 virus and its ability to hide out in our bodies long-term. Data has shown viral shedding in some people lasting as long as eighty-three days out from the first day of symptoms.   What Studies Show Some of the recovered/discharged patients showed a positive viral RNA burden for as long as 10 to 27 days after the discharge (Korber et al., 2020; Ye et al., 2020) Although the median viral shedding duration was 20 days, in some cases, it was observed for 37 days (Zhou et al., 2020). The prolonged SARS-CoV-2 RNA shedding occurred with a median duration of 53 days and a maximum of 83 days (Li, Wang, et al., 2020). In addition to this prolonged carriage of SARS-CoV-2, some patients who had recovered from COVID-19 demonstrated recurrence of SARS-CoV-2 (Xiao et al., 2020; Ye et al., 2020; Yuan et al., 2020). In one study, as much as 9.1% of the discharged COVID-19 patients were shown to be presented with the SARS-CoV-2 reactivation (Ye et al., 2020). Another study revealed that 14.5% of discharged COVID-19 patients with negative (polymerase chain reaction) PCR, had a later positive reverse transcription PCR (Yuan et al., 2020). Still another study indicated that the number of such recurring patients can be as high as 21.4% (Xiao et al., 2020). Another explanation is genetic mutations causing new strains. Sarah explains this is not likely. While there variants, they are not different enough to be considered a new strain.   What Explains Covid-19 Reactivation? Sarah sums up that the most likely explanation for the resurgence of symptoms is due to the virus's ability to hide inside our bodies. (36:03) She goes a bit more in-depth on how exactly a virus can hide from our immune systems. One of the implications of this is that we could have more lasting immunity from a vaccine than natural infection. It may hide out in exosomes within cells, which are little tiny bubbles inside the cell membrane. What this all data points to is herd immunity through natural infection is unlikely. And that we may be able to achieve a more lasting immunity through vaccination. Links to the Studies Sarah Recommends: https://www.frontiersin.org/articles/10.3389/fimmu.2020.01949/full https://www.tandfonline.com/doi/full/10.1080/07391102.2020.1790426 https://pubmed.ncbi.nlm.nih.gov/32838134/ https://pubmed.ncbi.nlm.nih.gov/32340768/ https://pubmed.ncbi.nlm.nih.gov/32619697/   Long-Haulers Stacy references the Scientific Method from a previous episode and explains how we're still mid-process. (40:41) Sarah wants to acknowledge listeners that this doesn't feel like good news. The main message that this scientific research has for Sarah is that the efforts going in to protect herself and others are still really important to do, even though we may be getting tired of it. The science behind wearing masks to slow the spread just gets stronger and stronger. It's potentially a large percentage of people who fall into the long-hauler or long-Covid category. Over 30% of long-haulers still have symptoms at 3 weeks, and approximately 10% still have symptoms at 2 months. Sarah also notes that data might be lacking because not all long-haulers are seeking medical attention if their symptoms are tolerable. The most common symptoms for long-haulers are fatigue, ongoing shortness of breath, joint aches, muscle aches, brain fog, and difficulty focusing. Some people report feeling better for a few weeks, while others report not feeling better at all.   The Body's Response Sarah explains how autoimmune diseases work and how it relates to Chronic Fatigue Disorder, which shows similar symptoms to long-Covid. Some researchers think what's happening in long-haulers isn't exactly continued infection. But more of the infection melding into Chronic Fatigue. Another thing that could affect long-haulers is organ and tissue damage, which takes a long time to heal. Researchers have now started to note Multisystem Inflammatory Syndrome cases in some Covid patients.  This was thought to be more common in children but is showing up increasingly in adults.  MIS typically happens in people who had Covid and recovered from it. It's a very severe, life-threatening (but treatable) inflammatory syndrome. Clinical features in children have varied but include shock, cardiac dysfunction, abdominal pain, and elevated inflammatory markers. Adults show severe dysfunction of one or more extrapulmonary organ systems (e.g., hypotension or shock, cardiac dysfunction, arterial or venous thrombosis or thromboembolism, or acute liver injury); 4) laboratory evidence of severe inflammation (e.g., elevated CRP, ferritin, D-dimer, or interleukin-6);  Sarah sums up that in its severe form, patients can develop MIS. But in mild-form, patients show symptoms of long-Covid.   What This Means For Covid-19 Patients The way this is currently treated mostly by symptom relief, rest, and stress reduction. Stacy shares that until we know more and the science can catch up with medial solutions, we know what we need to do to at least feel better. And how to continue to reduce the spread. Stacy reminds us that drinking alcohol can exacerbate long-haul symptoms on the front end. Sarah explains that alcohol is actually the opposite that's needed to support immune function. Because we focus so much on the physical side of Covid-19, Stacy reminds us that there's a lot of mental health at play from anxiety. This anxiety comes from all the things we don't know.  Even if you've already had it, like Stacy, we don't know if we can get it again, if it will be worse the second time, or if we'll be asymptomatic and infect others. You're not alone. You're not the only one feeling frustrated and exhausted and over it and ready for change. We're on the same team. Sarah's Helpful Links: https://www.nature.com/articles/d41586-020-025986 https://jamanetwork.com/journals/jama/fullarticle/2771111 https://www.cdc.gov/mmwr/volumes/69/wr/mm6940e1.htm   Long-Term Damage From Covid-19 Because of the way Covid-19 is effecting our bodies, there's the potential for organ damage that's important for us to understand. (56:11) Sarah underlines that the most concerning piece of that is cardiac damage. Unlike the other longterm challenges from organ damage, it's something that happens independently of severity. Sarah talks about a study done in Germany that looked at MRIs of their hearts. What they found was abnormalities in 78 of the 100 participants with ongoing inflammation. They showed that this was independent of preexisting conditions. Sarah believes it's very important for people to understand that this illness isn't just about whether you live or die, or whether or not you need to be hospitalized. Sarah goes on to say that the unknown factors make the idea of herd immunity much scarier. By allowing the number of people needed to be infected for herd immunity, we're potentially putting a lot of people in danger of long-lasting complications from Covid-19.   Not Just The Heart Kidney damage has been seen in severe infections. Acute kidney injury (AKI) has been reported in up to 25% of critically-ill patients, especially in those with underlying comorbidities.  The type of pneumonia often associated with COVID-19 can cause long-standing breathing issues.  The resulting scar tissue can lead to long-term breathing problems. An Austrian study also found that lung damage lessened with time.  88% of participants had visible damage 6 weeks after being discharged from the hospital, but by 12 weeks, this number had fallen to 56% (see go.nature.com/3hiiopi). Sarah also tells us that research suggests Covid-19 does have an effect on the brain as well, but there is still a lot that doctors and scientists don't know. Studies show Covid-19 can cause strokes and seizures, even in young people. Covid-19 may also exacerbate and potentially increase the risk of developing Parkinson's disease and Alzheimer's disease. Sarah's Suggested Further Reading: https://www.sciencemag.org/news/2020/09/evidence-builds-covid-19-can-damage-heart-doctors-are-racing-understand-it https://www.hopkinsmedicine.org/health/conditions-and-diseases/coronavirus/coronavirus-kidney-damage-caused-by-covid19 https://www.mayoclinic.org/diseases-conditions/coronavirus/in-depth/coronavirus-long-term-effects/art-20490351 Final Thoughts Sarah explains that Covid-19 is the perfect storm of virus. (1:15:25) The reason this is something we've not really experienced in the last hundred years is because the asymptomatic and pre-symptomatic spread makes it very difficult to control. Sarah adds that the long span of time for infection adds to the difficulty. Sarah explains the difference between aerosol and respiratory droplets, and how masks prevent the virus from spreading. Stacy adds that it's not enough to wear a mask, but to make sure it's properly fitted to your face to keep you and others safe. Sarah also notes that 6 feet is not enough distance indoors, and that masks will still be needed even after a vaccine rolls out. Sarah takes a minute to remind about immune health. This means get enough sleep, manage stress, get activity, address vitamin D, eat a nutrient-dense diet, look after gut health, and avoid alcohol. This is the pattern that Sarah used for her mask, as well as the Fruit of the Loom Masks, and nose pieces. Here's Sarah's post for more information. Sarah also underlines how important masks are. They're our greatest defense and offense for controlling infection. Stacy adds that there is nothing political about wearing a mask either. She and Sarah are here to give the science needed to live life as healthy as possible.   A Big Thank You! Stacy would like to encourage everyone to have compassion and says thank you anyone continuing to fight the spread. Your efforts are appreciated! Sarah also expresses her gratitude toward the audience for following the science and taking action. Sarah and Stacy will be sharing more of their unfiltered thoughts on Patreon as they recap the episode. Thank you so much for being here today and being a part of this community we so appreciate. We'll be back next week (hopefully with something happier to talk about)!  

It’s a question that has troubled providers for years – how do you keep patients on track with behavior change between appointments? Longtime physician assistant and educator Ken Korber hit upon a novel idea: use story books to educate the patient’s young children about healthy behaviors, and turn them into “little health ambassadors” in the home. Korber, who is also president of the Association of Family Practice PAs and NPs and a Clinical Instructor at Mount St. Joseph University, says the books allow him to bridge his clinical experience with his passion for writing and education, and will hopefully improve the health of adults and kids alike. It’s also in keeping with the focus many PAs and NPs have on primary care and prevention. His latest book "Grace Fights COVID-19" is the first resource of its kind in the U.S. for parents and kids. He also has advice for newly minted PAs and NPs about their role on the healthcare team, so there’s lots to check out in this episode with host Dr. Rishi Desai.

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2020.07.26.219741v1?rss=1 Authors: Mansbach, R. A., Chakraborty, S., Nguyen, K., Montefiori, D., Korber, B., Gnanakaran, S. Abstract: The COVID-19 pandemic underwent a rapid transition with the emergence of a SARS-CoV-2 variant that carried the amino acid substitution D614G in the Spike protein that became globally prevalent. The G-form is both more infectious in vitro and associated with increased viral loads in infected people. To gain insight into the mechanism underlying these distinctive characteristics, we employed multiple replicas of microsecond all-atom simulations to probe the molecular-level impact of this substitution on Spike closed and open states. The open state enables Spike interactions with its human cellular receptor, ACE2. Here we show that changes in the inter-protomer energetics due to the D614G substitution favor a higher population of infection-capable (open) states. The inter-protomer interactions between S1 and S2 subunits in the open state of the D-form are asymmetric. This asymmetry is resolved in the G-form due to the release of tensile hydrogen bonds resulting in an increased population of open conformations. Thus, the increased infectivity of the G-form is likely due to a higher rate of profitable binding encounters with the host receptor. It is also predicted to be more neutralization sensitive due to enhanced exposure of the receptor binding domain, a key target region for neutralizing antibodies. Copy rights belong to original authors. Visit the link for more info

Perpetual Investments Head of Credit and Fixed Income; Perpetual Credit Income Trust (PCI) Portfolio Manager, Michael Korber speaks with Tom Piotrowski about the current status of the fixed income universe and his views on the current market conditions & recovery affected by the COVID-19 crisis. This report is approved and distributed in Australia by Commonwealth Securities Limited ABN 60 067 254 399, AFSL 238814 (CommSec) a wholly owned but non-guaranteed subsidiary of Commonwealth Bank of Australia ABN 48 123 123 124, AFSL 234945 (the Bank). The Bank and its subsidiaries have effected or may effect transactions for their own account in any investments or related investments referred to in this report. This report is not a recommendation to buy, sell or hold any securities or financial products, and has been prepared without taking account of the objectives, financial or taxation situation or needs of any particular individual. For this reason, any individual should, before acting on the information in this report, consider the appropriateness of the information, having regard to the individual's objectives, financial or taxation situation and needs and, if necessary, seek appropriate professional advice. This report is produced by Commonwealth Research based on information available at the time of publishing. We believe that the information in this correspondence is correct and any opinions, conclusions or recommendations are reasonably held or made as at the time of its compilation, but no warranty is made as to accuracy, reliability or completeness. To the extent permitted by law, neither the Bank nor any of its subsidiaries accept liability to any person for loss or damage arising from the use of this report.  

Hollister interviews Sabrina Korber, who was at Fox News, around all things Bombshell, which they saw together. Long before the Me Too movement. A film for women to be sure.

On this 116th episode of the Arlington-Prospect Advice Givers Podcast. Ken Korber, Founder of The Center For Functional Learning, is our guest. Ken takes us on a journey with Grace and her cast of friends in his series of published children’s books. Created based on the music note ‘Grace’, Ken’s stories are rooted in the...

#podcast |A l'occasion du 150e anniversaire du Grand Louis de Funès, Cinémaradio ressort La Saga De Funès d'Eric Desmet et s'intéresse à toute la vie et l'oeuvre de ce grand du cinéma français.#louisdefunes #defunes #cinema #cinemaradio #film #cine

Cinémaradio ressort La Saga De Funès d'Eric Desmet et s'intéresse à toute la vie et l'oeuvre de ce grand du cinéma français. #louisdefunes #defunes #cinema #cinemaradio #film #cine

Mettre la technologie spatiale au service des équipes de secours lors des grandes catastrophes, tel est l'objectif de la Charte internationale "Espace et catastrophes majeures". Initiée par le CNES et l’ESA en 1999, cette charte regroupe aujourd'hui 16 agences spatiales. Afin d’apporter au plus vite une aide aux populations sinistrées et aux équipes de secouristes engagées sur les zones touchées, les membres de la Charte assurent une veille permanente : après avoir vérifié la pertinence et l’honnêteté de la demande, l’objectif est de programmer des prises de vue spécifiques et de faire parvenir au plus vite des images aux personnes et aux services qui en ont le besoin. Les intervenantes : Claire Tinel et Déborah Korber

1- Brilliant guests: Jeff Korber, Owen Benjamin & Clayton Thomas 2- Don't ask me that 3- Preemptive impeachment 4- Constitution? We shot it. 5- where's my Oxy 6- Forget Oxy -- where's my heroin 7- I'm Tom Brady. Please don't like me 8- Don't steal from Superwoman

Jedi, Kevin, and Korber talk about gaming in the second half of 2011

Jedi, Kevin, and Korber talk about gaming in the second half of 2011

Hillis and Baird plus a little bit of Korber talk about E3.  Terrible audio problems persist for some comic relief.

"Eine Signalwirkung auf die osteuropäischen Staaten" - der Berliner Justizsenator Horst Korber bewertet den deutsch-deutschen SportvertragDie „Frankfurter Allgemeine Zeitung“ hat in ihrer Ausgabe vom 8. Mai 1984 resümiert: „Ein Jubiläum, aber kein Grund zum Feiern“. Auf den Tag genau waren zu diesem Zeitpunkt bereits zehn Jahre verstrichen, als man das deutsch-deutsche Sportabkommen unterzeichnete. Zwei Sondierungsgespräche und fünf Verhandlungsrunden waren notwendig, um einen Vertrag zwischen dem Deutschen Sportbund der BRD und dem Turn- und Sportbund der DDR unterschriftsreif auszuhandeln, der dann auch am 8. Mai 1974 unterzeichnet wurde. Eine „neue Ära“ der Sportbeziehungen zwischen beiden deutschen Staaten sollte nun eingeläutet werden, zumal sie seit 13 Jahren – also seit dem Bau der Mauer – völlig brach lagen. „Berlin-Ost“ und „Berlin-West“ beim bilateralen Sportverkehr Neben zahlreichen anderen, gab es bei den Verhandlungen einen besonders schwierigen Knackpunkt. Die bundesdeutsche Delegation bestand darauf, auch die Sportorganisation von West-Berlin uneingeschränkt in den bilateralen Sportverkehr einzubeziehen. Dem hat schließlich die DDR-Delegation zugestimmt, doch die geographische Bezeichnung für die geteilte Stadt bereitete nach wie vor einige Schwierigkeiten. Denn vor allem die durch die DDR geführte offizielle Bezeichnung für Berlin mit dem Zusatz „Hauptstadt der DDR“ war für die bundesdeutsche Delegation inakzeptabel. Schließlich einigte man sich auf die Bezeichnung „Berlin-Ost“ und „Berlin-West“. Noch bis zum Ende des Jahres 1974 sollten rund 50 Sportbegegnungen stattfinden, davon vier im Westen Berlins und fünf im Osten. Dabei hat die DDR-Führung niemals von „Freundschaftsspiel“ gesprochen, denn schließlich handelte es sich bei der BRD um den „Klassenfeind“. Zehn Jahre danach bilanzierte die „Frankfurter Allgemeine Zeitung“ am 8. Mai 1984: „Unter dem Strich bleibt nach zehn Jahren deutsch-deutscher Sportverkehr die nüchterne Erkenntnis der Unfähigkeit zweier nach Millionen zählenden Massenorganisationen, die ihnen angeschlossenen Menschen zueinander kommen zu lassen“. Am Tag der Vertragsunterzeichnung, also am 8. Mai 1974, sprach DW-Redakteurin Renate Deutsch mit dem Berliner Justizsenator Horst Korber über den deutsch-deutschen Sportvertrag und die Rolle West-Berlins bei diesem Abkommen. Andreas Zemke Redaktion: Diana Redlich

The PHO5 and PHO8 genes in yeast provide typical examples for the role of chromatin in promoter regulation. Both genes are regulated by the same transcriptional activator, Pho4, which initiates nucleosome remodeling and transcriptional activation. In spite of this co-regulation, there are important differences in gene activity and in the way promoter chromatin undergoes chromatin remodeling. First, PHO5 belongs to one of the most strongly induced genes in yeast being 10-fold more active than the PHO8 gene (Oshima, 1997; Barbaric et al., 1992). Second, chromatin remodeling at the PHO5 promoter affects four nucleosomes (Almer et al., 1986), whereas only two nucleosomes are afffected at the PHO8 promoter (Barbaric et al., 1992). Third, neither the histone acetyl transferase Gcn5 nor chromatin remodeling complex Swi/Snf seem to be critically required for chromatin remodeling at the PHO5 promoter (Barbaric et al., 2001; Reinke and Hörz, 2003; Dhasarathy and Kladde, 2005; Neef and Kladde, 2003). At the PHO8 promoter, on the other hand, absence of Swi/Snf results in the complete loss of chromatin remodeling under inducing conditions. Furthermore, Gcn5 is required for full remodeling and transcriptional activation at this promoter (Gregory et al., 1999). Ever since these differences were recognized there have been speculations about the underlying reasons. This work shows that these discrepancies are not a direct consequence of the position or strength of the UASp elements driving the activation of transcription. Instead, these differences result from different stabilities of the two promoter chromatin structures. The basis for these results was the development of a competitive yeast in vitro assembly technique in which differences in nucleosome stability between promoter regions could be directly compared. This technique originated from a yeast in vitro chromatin assembly system that generated the characteristic PHO5 promoter chromatin structre (Korber and Hörz, 2004). As shown here, this system also assembles the native PHO8 promoter nucleosome pattern. Using the competitive assembly system it was shown that the PHO8 promoter has greater nucleosome positioning power, and that the properly positioned nucleosomes are more stable than at the PHO5 promoter. This provided for the first time evidence for the correlation of inherently more stable chromatin with stricter co-factor requirements. Remarkably, the positioning information for the in vitro assembly of the native PHO5 and PHO8 promoter chromatin patterns was specific to the yeast extract. Salt gradient dialysis or Drosophila embryo extract assemblies did not support the proper nucleosome positioning. However, nucleosomes in chromatin generated in these systems could be shifted to their in vivo-like positions by the addition of yeast extract. This indicates that the nucleosome positioning mechanisms in vitro are uncoupled from the nucleosome loading machinery. The nucleosome positioning at the PHO5 and PHO8 promoters was energy dependent suggesting a role of chromatin remodeling machines in generation of the repressed promoter chromatin structure. In spite of this, the chromatin remodeling machines Swi/Snf, Isw1, Isw2 and Chd1 were dispensable nucleosome positioning at both promoters.