Podcasts about animal model

Today, Dr. Patrick Schloss, Professor in the Department of Microbiology and Immunology in the School of Medicine at the University of Michigan, joins the #QualityQuorum to discuss how the human microbiome is studied, possible pitfalls in such data analysis, and what tools he and his coworkers have developed to lead toward repeatable, hypothesis-driven science. Host: Mark O. Martin Guest: Patrick Schloss Subscribe: Apple Podcasts, Spotify Become a patron of Matters Microbial! Links for this episode An overview of how the gut microbiome is analyzed. One of the articles discussed by Dr. Schloss exploring reproducibility in microbiome studies: “Identifying and Overcoming Threats to Reproducibility, Replicability, Robustness, and Generalizability in Microbiome Research.” Another article discussed by Dr. Schloss, regarding the link between the microbiome and obesity:  “Looking for a Signal in the Noise:  Revisiting Obesity and the Microbiome.” An article from Dr. Schloss' research team that explores a link between the human microbiome and a type of colorectal cancer. A link to the MOTHUR project, used to analyze microbiome data. A link to a video by Dr. Schloss:  “Understanding Disease Through the Lens of the Microbiome.” Dr. Schloss' YouTube channel about data analysis. Dr. Schloss' research group website. Dr. Schloss' faculty website. Intro music is by Reber Clark Send your questions and comments to mattersmicrobial@gmail.com

BUFFALO, NY- July 10, 2024 – A new #research paper was #published in Aging (listed by MEDLINE/PubMed as "Aging (Albany NY)" and "Aging-US" by Web of Science) Volume 16, Issue 12, entitled, “Aging exacerbates oxidative stress and liver fibrosis in an animal model of Down Syndrome.” Down Syndrome (DS) is a common genetic disorder characterized by an extra copy of chromosome 21, leading to dysregulation of various metabolic pathways. Oxidative stress in DS is associated with neurodevelopmental defects, neuronal dysfunction, and a dementia onset resembling Alzheimer's disease. Additionally, chronic oxidative stress contributes to cardiovascular diseases and certain cancers prevalent in DS individuals. In this new study, researchers Sebastiano Giallongo, Jessica Ferrigno, Rosario Caltabiano, Giuseppe Broggi, Amer M. Alanazi, Alfio Distefano, Emanuela Tropea, Antonella Tramutola, Marzia Perluigi, Giovanni Li Volti, Eugenio Barone, and Ignazio Alberto Barbagallo from the University of Catania, King Saud University, and Sapienza University of Rome investigated the impact of aging on oxidative stress and liver fibrosis using a DS murine model (Ts2Cje mice). “Our results show that DS mice show increased liver oxidative stress and impaired antioxidant defenses, as evidenced by reduced glutathione levels and increased lipid peroxidation.” DS liver exhibited an altered inflammatory response and mitochondrial fitness as the researchers showed by assaying the expression of HMOX1, CLPP, and the heat shock proteins Hsp90 and Hsp60. DS liver also displayed dysregulated lipid metabolism, indicated by altered expression of PPARα, PPARγ, FATP5, and CTP2. Consistently, these changes might contribute to non-alcoholic fatty liver disease development, a condition characterized by liver fat accumulation. Consistently, histological analysis of DS liver revealed increased fibrosis and steatosis, as showed by Col1a1 increased expression, indicative of potential progression to liver cirrhosis. Therefore, their findings suggest an increased risk of liver pathologies in DS individuals, particularly when combined with the higher prevalence of obesity and metabolic dysfunctions in DS patients. “These results shed a light on the liver's role in DS-associated pathologies and suggest potential therapeutic strategies targeting oxidative stress and lipid metabolism to prevent or mitigate liver-related complications in DS individuals.” DOI - https://doi.org/10.18632/aging.205970 Corresponding author - Giovanni Li Volti - livolti@unict.it Video short - https://www.youtube.com/watch?v=8GlAruy0xfk Sign up for free Altmetric alerts about this article - https://aging.altmetric.com/details/email_updates?id=10.18632%2Faging.205970 Subscribe for free publication alerts from Aging - https://www.aging-us.com/subscribe-to-toc-alerts Keywords - aging, Down Syndrome, oxidative stress, liver About Aging-US The mission of the journal is to understand the mechanisms surrounding aging and age-related diseases, including cancer as the main cause of death in the modern aged population. The journal aims to promote 1) treatment of age-related diseases by slowing down aging, 2) validation of anti-aging drugs by treating age-related diseases, and 3) prevention of cancer by inhibiting aging. (Cancer and COVID-19 are age-related diseases.) Please visit our website at https://www.Aging-US.com​​ and connect with us: Facebook - https://www.facebook.com/AgingUS/ X - https://twitter.com/AgingJrnl Instagram - https://www.instagram.com/agingjrnl/ YouTube - https://www.youtube.com/@AgingJournal LinkedIn - https://www.linkedin.com/company/aging/ Pinterest - https://www.pinterest.com/AgingUS/ Spotify - https://open.spotify.com/show/1X4HQQgegjReaf6Mozn6Mc MEDIA@IMPACTJOURNALS.COM

Jonathan Lowe is one of the foremost experts on ozone therapy for animals in the world and the author of the first book of its kind, The Essential Guide to Ozone Therapy for Animals. He is an entrepreneur, public speaker, and the founder of the International Veterinary Ozone Therapy Summit – the first event of its kind designed to bring together thought leaders from around the world. His dedication to evidence-based medicine that works in synergy with the body's biological mechanisms to bring healing is at the core of his quest to see ozone therapy become a central modality in every veterinary clinic. He is also the founder of O3Vets which received the Innovation Award from the Innovative Veterinary Care Journal. He resides with his wife, five children, and a golden retriever near Lansing, Michigan. Topics covered in this episode: How Jonathan got started in Ozone Therapy Ozone Therapy and how it's slowly finding its way into veterinary medicine  Ozone research and clinical trials How Ozone works and what it's used for The clinical application of Ozone Therapy and deciding which modality to use for treatment The ease and portability of using Ozone Therapy in the house call setting Links and Resources: Visit the O3 Vets website to learn more about Ozone Therapy and upcoming trainings Register or learn more about The Virtual International Veterinary Ozone Therapy Summit Find O3 Vets on Facebook Find O3 Vets on LinkedIn Find O3 Vets on YouTube These studies give us a good glimpse into the range of information available on ozone therapy in veterinary medicine: Ozone therapy in veterinary medicine: A review Ozone and its derivatives in veterinary medicine: A careful appraisal  An Overview of Ozone Therapy in Equine – An Emerging Healthcare Solution Effects of subcutaneous injection of ozone during wound healing in rats Treatment with ozone/oxygen-pneumoperitoneum results in complete remission of rabbit squamous cell carcinomas Ozone Therapy in the Prevention of Dental Plaque Formation in Dogs Topical Application of Ozonated Oils for the Treatment of MRSA Skin Infection in an Animal Model of Infected Ulcer Intramuscular oxygen-ozone therapy in the treatment of acute back pain with lumbar disc herniation: a multicenter, randomized, double-blind, clinical trial of active and simulated lumbar paravertebral injection Intrauterine therapy with ozone reduces subclinical endometritis and improves reproductive performance in postpartum dairy cows managed in pasture-based systems Ozone in Medicine. The Low-Dose Ozone Concept and Its Basic Biochemical Mechanisms of Action in Chronic Inflammatory Diseases Ozone therapy: an overview of pharmacodynamics, current research, and clinical utility The House Call Vet Academy links: Find out about The House Call Vet Academy online CE course  Learn more about Dr. Eve Harrison  Learn more about 1-to-1 coaching for current & prospective house call, mobile, & concierge vets Get House Call Vet swag! Find out about the next House Call & Mobile Vet Virtual Conference Music: In loving memory of Dr. Steve Weinberg. Intro and outro guitar music was written, performed, and recorded by house call veterinarian Dr. Steve Weinberg. Thank you to our sponsors! Chronos This podcast is also available in video on our House Call Vet Cafe YouTube channel

We are all the product of a reproductive process, yet reproductive biology, or the study of the processes and mechanisms involved in reproduction, is not well understood. Deepening our understanding of reproductive biology is crucial to advancing assistive reproductive technologies (ART) and advancing our collective comprehension of inheritance and evolution. Our guests for this episode are a couple, and we mean a literal married couple, of reproductive biology experts. Dr. Pavla Brachova and Dr. Nehemiah Alvarez, both working in the Eastern Virginia Medical School's Department of Physiological Sciences. In their collaborative work they aim to better understand and characterize the role of RNA and cellular events that impact ovarian function in women. We learn about their work with oocytes, which are single cells that grow and mature within the ovary and once fertilized provide the foundations of an embryo capable of maturing to a new individual. They outline how they use digital PCR (dPCR) and other methods to monitor RNA regulation in single cells and how progressing this work and lead to potential RNA-based therapies. In Cassie's career corner we hear childhood stories from each guest and learn about their respective career paths, which eventually collided and merged. They share insights on the importance of having mentors experienced in your field, the challenges of shared job searching, and the joys of collaborating as a couple with shared scientific interests.Visit the Absolute Gene-ius page to learn more about the guests, the hosts, and the Applied Biosystems QuantStudio Absolute Q Digital PCR System. 

Loneliness and social isolation are known to cause several mental health issues, as the COVID-19 pandemic reminded us.  This can cause long-term difficulties and seriously impact the brain and overall brain function.By studying the different biological and behavioural effects of social isolation on mice, Dr Jing Liang and her team have recently identified a promising therapeutic with the power to reverse these changes, a major development in this field.Read the original research: https://www.nature.com/articles/s41598-022-09814-5Read the Research Outreach article here

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2023.07.02.547148v1?rss=1 Authors: Varela, R. B., Boeschen, S. L., Yates, N., Houghton, T., Blaha, C. D., Lee, K. H., Bennet, K. E., Kouzani, A. Z., Berk, M., Quevedo, J., Valvassori, S. S., Tye, S. J. Abstract: Treatment of refractory bipolar disorder (BD) is extremely challenging. Deep brain stimulation (DBS) holds promise as an effective treatment intervention. However, we still understand very little about the mechanisms of DBS and its application on BD. The present study aimed to investigate the behavioural and neurochemical effects of ventral tegmental area (VTA) DBS in an animal model of mania induced by methamphetamine (m-amph). Wistar rats were given 14 days of m-amph injections, in the last day animals were submitted to 20 minutes of VTA DBS in two different patterns: intermittent low frequency stimulation (LFS) or continuous high frequency stimulation (HFS). Immediately after DBS, manic-like behaviour and nucleus accumbens (NAc) phasic dopamine (DA) release were evaluated separately through open-field test and fast-scan cyclic voltammetry. Levels of NAc dopaminergic markers were evaluated by immunohistochemistry. M-amph induced hyperlocomotion in the animals and both DBS parameters reversed this alteration. M-amph increased DA reuptake time post-sham compared to baseline levels, and both LFS and HFS were able to block this alteration. LFS was also able to reduce phasic DA release when compared to baseline. LFS was able to increase dopamine transporter (DAT) expression in the NAc. These results demonstrate that both VTA LFS and HFS DBS exert anti-manic effects and modulation of DA dynamics in the NAc. More specifically the increase in DA reuptake driven by increased DAT expression may serve as a potential mechanism by which VTA DBS exerts its anti-manic effects. Copy rights belong to original authors. Visit the link for more info Podcast created by Paper Player, LLC

A wholesome episode about secure love, forming bonds under stress, and human connection through the lens of a grasslands specialist: the prairie vole. Photos and more are at our site. Primary Sources: NPR on the hot new vole research: https://www.npr.org/sections/health-shots/2023/01/27/1152009605/prairie-voles-oxytocin-love-hormone-bonding-study Why Prairie Voles are our animal model: The Prairie Vole (Microtus ochrogaster): An Animal Model for Behavioral Neuroendocrine Research on Pair Bonding. Wild Prairie Vole Social Organization: Social Organization of the Prairie Vole (Microtus ochrogaster) Contact Website Facebook Twitter info@grasslandgroupies.org

Spironolactone maybe safe in pregnancy - Niacinamide riboside: Worse cancer in an animal model - TikTok has lots of info about AD--but at what cost? - Non-invasive treatments for calcinosis cutis - Behavioral economics in healthcare: Anchoring--with Steve Feldman! Connect with us! Web: https://dermaspherepodcast.com/ Twitter: @DermaspherePC Instagram: @dermaspherepodcast Facebook: https://www.facebook.com/DermaspherePodcast/ Check out Luke and Michelle's other podcast, SkinCast! https://healthcare.utah.edu/dermatology/skincast/ Luke and Michelle report no significant conflicts of interest… BUT check out our friends at: Kikoxp.com (a social platform for doctors to share knowledge) https://www.levelex.com/games/top-derm(A free dermatology game to learn more dermatology!) The University of Utah Dermatology Echo: https://physicians.utah.edu/echo/dermatology-primarycare

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2022.10.24.513470v1?rss=1 Authors: Maisterrena, A., de Chaumont, F., Longueville, J.-E., Balado, E., Ey, E., Jaber, M. Abstract: Autism Spectrum Disorder (ASD) is a progressive neurodevelopmental disorder characterized mainly by deficits in social communication and stereotyped and restricted interests. Deficits in social interactions in ASD animal models are generally analysed using the three chambers test paradigm that is simple to implement and use but fails to detect subtle social deficits or complex social behavior on an extended period of time within a group of mice. Here, we set up a novel procedure entitled the Live Mouse Tracker (LMT) that detects a great number of complex social behaviours that we recorded continuously for up to three days in groups of 4 mice. This was performed in the valproic acid (VPA) mouse model where VPA (450 mg/kg) was injected to pregnant females at E12.5. Studies were performed with a special focus on females given that ASD is 3-4 times more diagnosed in males than in females and that several ASD models failed to detect major social deficits in females, contrary to males. Comparisons were made within groups of 4 female animals with same treatment or within groups of different treatments (saline versus VPA). We report that VPA females show several types of social deficits and that are different in nature and magnitude in relation with time (from 1 hour to 3 days). These deficits were also different when VPA mice were tested together compared to when they were mixed with saline treated mice. Indeed, while social behavior was improved in VPA mice with the presence of saline mice while that of saline mice was negatively affected by the presence of VPA mice. This study indicates that female VPA mice show several social deficits, contrary to the common knowledge. It further implies that ASD related behavior alters normal behavior in a mixed group of mice. Copy rights belong to original authors. Visit the link for more info Podcast created by Paper Player, LLC

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2022.09.14.507883v1?rss=1 Authors: Iglesias, L. P., Coelho, A. A., da Silva, N. R., Muller, H. K., Moreira, F. A., Wegener, G., Joca, S. Abstract: BACKGROUND: Post-traumatic stress disorder (PTSD) and co-morbid depression are frequently associated with severe symptoms, poor response to treatment and worse prognosis. Due to the absence of a suitable animal model, little is known about the biological basis of the comorbidity, severely limiting the discovery of new and more effective treatment options. The Flinders Sensitive Line rats (FSL) is a well-validated, selectively bred animal model of depression. However, several of its features, such as cognitive deficits and altered hypothalamic-pituitary-adrenal (HPA) axis response, also match symptomatic clusters of PTSD. In parallel, its resistant counterpart, the Flinders Resistant Line (FRL), is extensively used as a simple control. Still, little is known about its performance compared to the original strain, Sprague Dawley (SD), from which the FSL/FRL was originally derived. AIMS: Characterizing the behavioural performance and mechanisms involved in FSL, FRL and SD rats in fear-memory paradigms. METHODS: FSL, SD and FRL animals were submitted to tests assessing hippocampal-dependent and fear-related memory. Subsequently, plasticity factors and endocrine responses to stress were analysed to elucidate the molecular basis for the observed behavioural alterations. RESULTS: We found that FRL animals presented intact recognition memory and innate fear responses but could not properly display conditioned responses in the Conditioned Fear Conditioning (CFC) paradigm. FSL animals, despite a poor performance in the Novel Object Recognition task (NOR), showed similar levels of conditioned responses compared to SD, but impairments in extinction learning, a feature highly related to PTSD. The behavioural alterations were accompanied by alterations in plasma corticosterone levels and hippocampal expression of the glucocorticoid receptor and FKBP51. CONCLUSION: For the first time, we demonstrate an animal model of resilience and vulnerability to PTSD and co-morbid depression. The results suggest that the endophenotypes may be based on aberrant endocrine stress responses in the hippocampus. Copy rights belong to original authors. Visit the link for more info Podcast created by PaperPlayer

This episode explores how to support your loved one when they're struggling with an eating disorder. I talk through the New Maudsley Animal Model - the wonderful model created by the Maudsley Eating Disorder Service in London, UK. The model uses animal metaphors to educate and support carers in their communication with their loved one.   Being a carer is hugely challenging. With the best of intentions, we can go off track with our communication and inadvertently strengthen the ed voice. The Model simplifies communication and empowers carers with the skills to create a motivational and hopeful environment for change. I also talk through some of the nuances of practically putting this Model into practice, based on unexpected personal experience recently of supporting someone close to me.  I hope that you enjoy the episode. To find out more about the New Maudsley Animal Model: https://thenewmaudsleyapproach.co.uk/pdfs/AnimalAnalogies.pdf

Our guest on today's episode is Dr Nancy Padilla-Coreano. Dr Padilla-Coreano is a Professor at the University of Florida. There she runs her own lab and mainly investigates the neural mechanisms that drive social behaviours and dominance in animal models. She was selected as one of the L'Oreal For Women in Science 2020 Fellows and received the inaugural Henry Grass MD Rising Stars in Neuroscience Award back in 2021. She is joining us today to help us break down how our brains allow us to navigate social spaces. - You can find Dr Padilla-Coreano on Twitter @DrNancyPadilla - Academic Profile: https://directory.ufhealth.org/padilla-coreano-nancy - Discussed Publications: https://www.nature.com/articles/s41586-022-04507-5 https://royalsocietypublishing.org/doi/10.1098/rstb.2020.0444 - Keywords: Social Behavior, Science Advocacy, Animal Model, Imposter Syndrome, Electrophysiology

A state-of-the-art research and development centre in Daegu, Korea – K-MEDI hub – is creating a medical industry ecosystem for the research and development of new drugs and medical devices. At its Preclinical Research Centre, one strong research focus is evaluating the impact of stress on the welfare of laboratory animals in the context of preclinical research. The aim is to improve international standards and guidelines for experimental conditions in the future, improving both the welfare of laboratory animals and the quality of research results.Read their original research: http://doi.org/10.1101/2021.08.27.457934Read more in Research Features

Mouse models, based on dietary, chemical or genetic interventions, are designed to serve as tools to understand human liver diseases.With the number of models increasing:What types of models exist for hepatocellular carcinoma?How close do they mirror human liver cancer (subtypes)?Can they be used to predict therapeutic response?What are the best models? For which questions?From idea to conception, are we getting lost in translation?Read this recent Journal of Hepatology article, on which the discussion was be based.Read moreAll EASL Studio Podcasts are available on EASL Campus.

Because of natural exposure to wildfire smoke, nonhuman primates have provided an increased understanding of the long-term effects of smoke inhalation during infancy, shares Lisa Miller, University of California Davis (UC Davis). Dr. Miller also discusses with co-hosts Anne Chappelle and David Faulkner the importance of animal models in human health research and how nonhuman primates can be good models for vaccine testing, as was the case with COVID-19. About the GuestLisa A. Miller, PhD, is a Professor for the UC Davis School of Veterinary Medicine and serves as the Respiratory Diseases Unit Leader for the California National Primate Research Center. She also is the principal investigator or co-investigator for 15 active research grants.Dr. Miller's research focuses on investigating the impact of environmental exposures (air pollution, allergens, microbes) on pulmonary and immune system development during the first year of life. She uses both cell culture approaches and animal models to address questions related to mucosal immune mechanisms in pediatric populations, with an emphasis on understanding the etiology of childhood asthma and susceptibility to infectious disease.Dr. Miller earned her BS and PhD from UC Davis and was a postdoctoral fellow in the Stanford University School of Medicine.

How to get from “data” to “device”: one cool story.What data you need, where you get it – and most importantly – what you do with it, all determine whether a medical device will be successful. Of course, every technology has its own set of caveats, but it always helps to hear from people who have been there.In this episode, VP of Business Development Andy Rogers, and Senior Electrical Engineer/Partner Jake Cowperthwaite, both of Key Tech, talk with Steve Schaefer, CEO at CoolTech, about the quest for data with CoolStat: a new way to manage patient temperature in fever that can develop following a stroke, traumatic brain injury, seizure, or metabolic encephalopathy. There are other technologies that manage patient temperature, but CoolTech partnered with a engineering team to build a way build a better mousetrap. CoolStat generates filtered air that's delivered to the patient via a nasal mask air tubing set. It cools through evaporative cooling, using room temperature air. CoolStat is smaller and lighter than existing devices; it reduces side effects like shivering which can lead to complications – and shortens treatment time. Need to know:●       Understand the commercial product requirements before you start●       Your regulatory path depends on your specific device●       Go where the data leads The nitty-gritty: The initial data that drove CoolStat's development was collected from tests on pigs, who have similar physiology to humans.  In this case, because they were seeking objective data – rate of cooling, rate of air flow and efficiency of cooling – CoolTech was able to save time by using existing temperature probes and storage software.  The first results were okay, but less than optimal. For many companies, this can be a go/no-go point, where you decide to fish or cut bait. CoolTech opted to pause the study, make changes, and go back to the FDA with an improved device. Finding human subjects for testing was another challenge. Patients are typically unconscious in the ICU, so getting family consent is laborious, especially during the COVID pandemic. Patient data is recorded on CRF's and validated within 24 hrs. so engineers can quickly make changes based on this real-world info.   CoolTech found that partnering with outside resources, such as the National Institutes for Health (NIH) university hospitals, and end-user associations can help to expedite development. Clinical studies will be completed soon. Now the critical numbers for CoolStat are the savings that hospitals can reap using this exciting new technology.  Listen in for more data. And more details. HELPFUL LINKS:https://www.cooltechcorp.com/

Dr. Ethan Russo returns to the podcast to help us make sense of the slew of studies and articles claiming cannabis can treat and/or prevent COVID-19 infection. Dr. Russo is a board-certified neurologist, a psychopharmacology researcher, an author, a lecturer, and a consultant. He is a CannMed 2022 Keynote Presenter, where he will present on Cannabis and Psychiatry. Although Dr. Russo is not a virologist, he is a very accomplished medical cannabis researcher, and he is well-qualified to help us find the signal in all the noise. During the conversation, we discuss:  What role the endocannabinoid system plays in preventing and clearing a viral infection The different levels of clinical research The importance of peer review process The University of Chicago study that claims CBD inhibits SARS-CoV-2 replication The Oregon University Study that claims cannabinoid acids can prevent covid infection Animal models showing CBD can calm the cytokine storm A preprint study claiming CBD primes the innate immune system Claims that cannabis smokers have a greater risk for COVID-19 infection  Thank You to This Episode's Sponsor: Project CBD Founded in 2010, Project CBD is the first and longest-running website dedicated to research and education on the benefits of CBD, and the premier destination for both curious and committed CBD consumers. Often the first to report on cutting-edge health issues, Project CBD reporting reflects a deeper understanding of developments in cannabinoid science and therapeutics. Learn more at ProjectCBD.com  Additional Resources Cannabidiol inhibits SARS-CoV-2 replication through induction of the host ER stress and innate immune responsesCannabinoids Block Cellular Entry of SARS-CoV-2 and the Emerging VariantsEvaluation of Serum Cytokines Levels and the Role of Cannabidiol Treatment in Animal Model of AsthmaIn search of preventive strategies: novel high-CBD Cannabis sativa extracts modulate ACE2 expression in COVID-19 gateway tissuesCannMed ArchiveCannMed Community Board [Facebook Group]Healthcare Provider Medical Cannabis Research Study

In this episode of the Sentient Media Podcast, we meet Dr. Aysha Akhtar and hear about her work building a world where animal testing is no longer the default. Join our conversation and find out the facts about animal testing and what the future looks like without it. Aysha's work:https://ayshaakhtar.comhttps://contemporarysciences.org Aysha's articles on Sentient Media: https://sentientmedia.org/this-new-bill-could-phase-out-animal-testing-for-good/https://sentientmedia.org/to-avoid-the-next-pandemic-we-need-to-stop-factory-farming/https://sentientmedia.org/to-love-and-mourn-an-animal/Hit subscribe and find us here: Newsletter: https://sentientmedia.org/newsletter​ Facebook: https://facebook.com/sentientmediaorg​ Twitter: https://twitter.com/sentient_media​ Instagram: https://instagram.com/sentient_media​ LinkedIn: https://www.linkedin.com/company/sent...​Youtube: https://www.youtube.com/channel/UCudaVg7_tLsKzdg0fWko2bg 

Welcome to The Sports Docs Podcast with Dr. Ashley Bassett and Dr. Catherine Logan. On each episode we chat about the most recent developments in sports medicine and dissect through all the noise so you know which literature should actually impact your practice.  On today's episode we're focusing on blood flow restriction therapy or “BFR”. Blood flow restriction is one type of ischemic therapy that is thought to increase muscle mass, strength and performance at a lower level of resistance training. And that last part really is the key, because training at high loads is often not possible for our patients undergoing rehab for an injury or postoperatively. BFR involves the use of a cuff or tourniquet system positioned at the upper part of the limb to restrict venous blood return while maintaining arterial inflow. While the exact mechanism of BFR remains unclear, restriction of venous outflow leads to an anaerobic – or oxygen depleted – environment similar to that of higher-intensity training. This anaerobic environment is hypothesized to promote muscle hypertrophy through a combination of cell signaling and hormonal changes.  BFR is not for every patient and there are relative contraindications to its use. These include conditions that may increase the risk of blood clotting, such as vascular disease, obesity, sickle cell trait or disease, cancer and a history of DVT.Article 1: Blood Flow Restriction Training for Athletes: A Systematic Reviewhttps://journals.sagepub.com/doi/abs/10.1177/0363546520964454Article 2:Local and Systemic Effects of Blood Flow Restriction Therapy in an Animal Model https://pubmed.ncbi.nlm.nih.gov/33136456/Article 3:Blood Flow Restriction Training for the Shoulder: A Case for Proximal Benefithttps://pubmed.ncbi.nlm.nih.gov/34110960/

Commentary by Dr. Valentin Fuster

John Hasenau, DVM and Jeffrey Zynda consider practical options for housing and infrastructural designs to sustainably improve animal welfare, data reporting and reproducibility.

John Hasenau, DVM and Jeffrey Zynda consider practical options for housing and infrastructural designs to sustainably improve animal welfare, data reporting and reproducibility.

Commentary by Dr. Giselle Melendez

Tendinopathy is a debilitating tendon disorder that affects millions of Americans and costs billions of health care dollars every year. High mobility group box 1 (HMGB1), a known tissue damage signaling molecule, has been identified as a mediator in the development of tendinopathy due to mechanical overloading of tendons in mice. Metformin (Met), a drug approved by the Food and Drug Administration used for the treatment of type 2 diabetes, specifically inhibits HMGB1. This study tested the hypothesis that Met would prevent mechanical overloading-induced tendinopathy in a mouse model of tendinopathy created by intensive treadmill running (ITR). In conclusion, inhibition of HMGB1 by injections of Met prevented tendinopathy development due to mechanical overloading in the Achilles tendon in mice.   To view the article click here.

Blood flow restriction therapy (BFRT) has been increasingly applied to improve athletic performance and injury recovery. Validation of BFRT has lagged behind commercialization, and currently the mechanism by which this therapy acts is unknown. BFRT is one type of ischemic therapy, which involves exercising with blood flow restriction. Repetitive restriction of muscle blood flow (RRMBF) is another ischemic therapy type, which does not include exercise. In conclusion, ischemic therapy did not induce gains in muscle mass, contractility strength, fiber cross-sectional area, or satellite cell density locally or systemically in this model, although the RRMBF group did have elevated GH levels on ELISA.   Click here to read the article.

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2020.09.09.289348v1?rss=1 Authors: Mollinedo-Gajate, I., Song, C., Sintes-Rodriguez, M., Whelan, T., Soula, A., Selimbeyoglu, A., Hurley, S., Knöpfel, T. Abstract: Autism spectrum disorder (ASD) is characterized by core deficits in social interaction. The classic serotonergic psychedelic psilocybin has been suggested as a therapeutic agent that may ameliorate in the core symptomology of ASD. We found that the acute response to psilocybin was attenuated in the prenatal valproic acid exposure mouse model of ASD, and importantly, psilocybin rescued the social behavioural abnormalities present in these ASD model mice. Copy rights belong to original authors. Visit the link for more info

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2020.08.19.256396v1?rss=1 Authors: Dziorny, A. C., Scott, L. L., Luebke, A. E., Walton, J. P. Abstract: Congenital sensorineural hearing loss (SNHL) affects thousands of infants each year and results in significant delays in speech and language development. Previous studies have shown that early exposure to a simple augmented acoustic environment (AAE) can limit the effects of progressive SNHL on hearing sensitivity. However, SNHL is also accompanied by, hidden hearing loss, that is not assessed on standard audiological exams, such as reduced temporal processing acuity. To assess whether sound therapy may improve these hidden deficits, a mouse model of congenital SNHL was exposed to simple or temporally complex AAE. Peripheral function and sound sensitivity in auditory midbrain neurons improved following exposure to both types of AAE. However, only exposure to a novel, temporally complex AAE significantly improved a measure of temporal processing acuity, neural gap-in-noise detection in the auditory midbrain. These experiments suggest that targeted sound therapy may improve hearing outcomes for children suffering from congenital SNHL. Copy rights belong to original authors. Visit the link for more info

0:00-11:10- Dr. Gary Luker and Adeline Boettcher discuss their paper "Transitioning to a New Normal after COVID-19: Preparing to Get Back on Track for Cancer Imaging" recently publish in Radiology: Imaging Cancer. 11:11-26:04- Dr. Luke Wilkins interviews Medical Student Grant recipient Monica Matsumoto and her mentor Dr. Samdeep Mouli on their recent grant Y90 Radioembolization Combined with Immune Checkpoint Blockade in an Animal Model of Hepatocellular Carcinoma.

Smoking sucks...but Nicotine...ehh might not be so bad Notes: What is POTS?: https://www.webmd.com/heart-disease/atrial-fibrillation/postural-orthostatic-tachycardia#1 Benefits of Nicotine: https://www.nootropedia.com/benefits-of-nicotine/ Nicotine, The Wonder Drug: https://www.discovermagazine.com/health/nicotine-the-wonder-drug Self Hacked Nicotine: https://selfhacked.com/blog/28-proven-health-benefits-nicotine-4-potential-risks/ Ugh Fields: https://www.lesswrong.com/posts/EFQ3F6kmt4WHXRqik/ugh-fields Immunosuppressive and Anti-Inflammatory Effects of Nicotine Administered by Patch in an Animal Model: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC404586/ Is Nicotine All Bad?: https://www.scientificamerican.com/article/is-nicotine-all-bad/ Is Nicotine Bad for you? : https://veppocig.com/pages/is-nicotine-bad-for-you/ Twins who smoke: https://www.trueactivist.com/this-is-what-7-smoker-vs-non-smoker-identical-twins-look-like-after-years-of-lighting-up/ Benefits of Nicotine Study: https://www.ncbi.nlm.nih.gov/pubmed/1859921 Monster Thread on Nicotine Effects: https://raypeatforum.com/community/threads/nicotine-through-a-peat-prism.9898/ Nicotine increases insulin sensitivity in Mice: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3520975/ Nicotinic Acetylcholine Receptors: https://en.wikipedia.org/wiki/Nicotinic_acetylcholine_receptor The Dangers of Quitting Smoking by Eric Berg: https://www.youtube.com/watch?v=uQP3Gq-IGbA Products: Nicotine Patch: https://amzn.to/38WDIXF Nicotine Lozenge: https://amzn.to/2REzVbW Music by Jenny Jahlee from Live at KBOO

Helen Lee Breton is a researcher who works in Dr. Chen's lab. Scientists at Texas Biomed are using CRISPR technology to try to create a new animal model for liver cancer. Liver cancer can have its roots in infectious diseases or metabolic conditions. And it’s a killer worldwide. Promising therapies developed in mouse models have failed in humans. So the experts at the Southwest National Primate Research Center think a bigger animal like a monkey might work better. The Southwest National Primate Research Center at Texas Biomed is home to more than a thousand of these Old World monkeys. CRISPR came on to the scientific scene in 2012. Christopher Chen, Ph.D., says it’s really making a huge impact in labs around the country.  

Dr. Kelly Brogan - A Mind of Your Own - Podcast #165 Get Show Updates Here: http://www.beyondwellnessradio.com/newsletter You-tube Podcast Subscribe: http://www.youtube.com/subscription_center?add_user=justinhealth Show Transcription: https://justinhealth.com/dr-kelly-brogan-a-mind-of-your-own-podcast-165 Discover some natural ways to be more productive and learn about the different ways to address brain and gut inflammation. Also, stay tuned for some more information about Dr. Kelly's bestselling book, “A Mind of Your Own” and viral articles. In today's video, Dr. Kelly Brogan, an accomplished doctor and author of the New York Times bestselling book “A Mind of Your Own”, joins Dr. Justin Marchegiani as they both discuss the link between gut issues and mental health. Get some useful tips on how to keep your mind clear and active without gut issues hindering it. Get your own mind back with the help of functional medicine. Let's watch and listen! In this episode, we cover: 02:41   Depression: Illness of Modern Civilization, Not a Chemical Imbalance 04:53   Animal Model of Depression 08:20   Multiple Different Lifestyle Pillars 12:00   Meditation and Productivity 16:42   Supplemental Ways to Address Brain Inflammation Subscribe on I-Tunes: http://www.beyondwellnessradio.com/itunes Review us at: http://www.beyondwellnessradio.com/itunes Visit us at: http://www.beyondwellnessradio.com Have a question: http://www.beyondwellnessradio.com/question

This podcast covers the JBJS issue for May 2017. Featured are articles covering Synovial Fluid Cell Count for Diagnosis of Chronic Periprosthetic Hip Infection; recorded commentary by Dr. Bibbo; Return to Play and Patient Satisfaction After ACL Reconstruction - Study with Minimum 2-Year Follow-up; Leptin Elevation as a Risk Factor for SCFE Independent of Obesity Status; recorded commentary by Dr. Adamczyk; Sclerostin Antibody Enhances Rotator Cuff Tendon-to-Bone Healing in an Animal Model.

This podcast covers the JBJS issue for May 2017. Featured are articles covering Synovial Fluid Cell Count for Diagnosis of Chronic Periprosthetic Hip Infection; recorded commentary by Dr. Bibbo; Return to Play and Patient Satisfaction After ACL Reconstruction - Study with Minimum 2-Year Follow-up; Leptin Elevation as a Risk Factor for SCFE Independent of Obesity Status; recorded commentary by Dr. Adamczyk; Sclerostin Antibody Enhances Rotator Cuff Tendon-to-Bone Healing in an Animal Model.

How does radio reach out of the studio? This week, we tune in explore the science and technology of broadcasting to find out how a voice hits the airwaves. We discover the difference between AM, FM and DAB, and use basic physics to build our own microphone. Plus, the 7000 year old cheese and the surprisingly simple solution to a box jellyfish sting. Like this podcast? Please help us by supporting the Naked Scientists

How does radio reach out of the studio? This week, we tune in explore the science and technology of broadcasting to find out how a voice hits the airwaves. We discover the difference between AM, FM and DAB, and use basic physics to build our own microphone. Plus, the 7000 year old cheese and the surprisingly simple solution to a box jellyfish sting. Like this podcast? Please help us by supporting the Naked Scientists

Geoff Butcher has Parkinson's disease. Here we hear him interview a scientist who uses Marmosets as an animal model to investigate Parkinson's disease. The scientist does this by using a chemical called MPTP to destroy the substantia nigra in the Marmosets. This is the part of the brain that is associated with the fine control of movement. It is damage to the substantia nigra that caused the symptoms of Parkinson's disease. The discovery of MPTP was an accident. Drug-users took contaminated material and developed Parkinsonian-like symptoms. This led to the unravelling of a medical mystery described in The Case of The Frozen Addicts reviewed here: http://www.nejm.org/doi/full/10.1056/NEJM199612263352618

Mon, 23 Jan 2012 12:00:00 +0100 https://edoc.ub.uni-muenchen.de/13985/ https://edoc.ub.uni-muenchen.de/13985/2/Radic_Tamara.pdf Radic, Tamara ddc:61

Background: Most studies on biodegradable magnesium implants published recently use magnesium-calcium-alloys or magnesium-aluminum-rare earth-alloys. However, since rare earths are a mixture of elements and their toxicity is unclear, a reduced content of rare earths is favorable. The present study assesses the in vivo biocompatibility of two new magnesium alloys which have a reduced content (ZEK100) or contain no rare earths at all (AX30). Methods: 24 rabbits were randomized into 4 groups (AX30 or ZEK100, 3 or 6 months, respectively) and cylindrical pins were inserted in their tibiae. To assess the biodegradation mu CT scans and histological examinations were performed. Results: The mu CT scans showed that until month three ZEK100 degrades faster than AX30, but this difference is leveled out after 6 months. Histology revealed that both materials induce adverse host reactions and high numbers of osteoclasts in the recipient bone. The mineral apposition rates of both materials groups were high. Conclusions: Both alloys display favorable degradation characteristics, but they induce adverse host reactions, namely an osteoclast-driven resorption of bone and a subsequent periosteal formation of new bone. Therefore, the biocompatibility of ZEK100 and AX30 is questionable and further studies, which should focus on the interactions on cellular level, are needed.

Mice can be used to mimic Alzheimer's disease in humans. Alzheimer's disease is the most common type of dementia. Currently there are no effective treatments so mice models offer one approach to both understanding and developing treatments for Alzheimer's. Here we see mice digging and burrowing. Find more information about Alzheimer's disease here: http://alzheimers.org.uk/ More science here: Deacon, R.M.J. Digging and marble burying in mice: simple methods for in vivo identification of biological impacts. Nat. Protocols 1, 122 - 124 (2006). http://www.nature.com/nprot/journal/v1/n1/full/nprot.2006.20.html

Our ageing society is confronted with a dramatic increase in patients suffering from tauopathies such as Alzheimer's disease, frontotemporal dementia and others. Typical neuropathological lesions including tangles composed of hyper-phosphorylated tau protein as well as severe neuronal cell death characterize these disorders. No mechanism-based cures are available at present. Genetically modified animals are invaluable models to understand the molecular disease mechanisms and to screen for modifying compounds. We recently introduced tau-transgenic zebrafish as a novel model for tauopathies. Our model allows recapitulating key pathological features of tauopathies within an extremely short time. Moreover, life imaging of tau-dependent neuronal cell death was performed for the very first time. This demonstrated tau-dependent neuronal cell loss independent of tangle formation. Finally, we exemplified that the zebrafish frontotemporal dementia model can be used to screen for drugs that prevent abnormal tau phosphorylation and neuronal cell death. Copyright (C) 2010 S. Karger AG, Basel

Appropriately sized arteries in small animals may be possible models for studying the remodeling process as occurs after arterial balloon injury in humans. Magnetic resonance imaging (MRI) is able to noninvasively image tissue in vivo. To date, small animal angiog raphy models have mostly used research-dedicated instruments and resolution, which are not universally available.Experiments were carried out on a rat aorta model of remodeling in vivo (n=40). Arteries were injured by oversized balloon dilation; control arteries were uninjured. Angiography imaging was performed immediately before sacrifice with an unmodified clinical MRI unit, a 1.5 Tesla MR tomograph with a 20-cm-diameter coil. Longitudinal MRI pictures of the aorta and morphometry of tissue sections to measure luminal and arterial wall areas were analyzed with use of computer-assisted techniques.Comparison of dimensions demonstrated correlation between MRI and histology measurements of the lumen. MRI and morphometry showed a gradual increase in mean luminal area over 6 weeks following injury. The lumen increase correlated with total arterial area and thickness.In this rat aorta model, remodeling documented at histology was followed-up in vivo. The use of such clinical MRI scanners has potential to reduce animal numbers needed to follow-up the remodeling process after therapeutic intervention.

Fri, 1 Jan 1993 12:00:00 +0100 https://epub.ub.uni-muenchen.de/6908/1/chronopharmacology_of_mitoxantrone_6908.pdf Emmerich, B.; Kammermeier, I.; Langenmayer, Irmgard; Levi, F.; Hallek, M.