Podcasts about Danio

In today's episode, "Fish in Space!", the Gettin' Fishy Crew travel into the great beyond with Dr. Moiya McTier, astrophysicist, folklorist and host of the podcast Pale Blue Pod! We chat about different “fish-stronauts” who have traveled to space for scientific purposes and we get to learn about some very interesting fish related folklore about the stars. Did you know that the mummichog was the first fish into space? Do you not know what a Mummichog is? Did you know that in some cultures, a celestial fish gave birth to the galaxies? Come find out! This podcast is brought to you by Danio margaritatus, or the celestial pearl danio, otherwise known as the galaxy rasbora. These fish are located in a very small part of Burma, Southeast Asia and have become a mainstay in the hobby due to their bright colors and small size. Even though this tiny marvel is named after the heavens, it has sadly never been to space.  It has been said that each galaxy danio looks up into the night sky and yearns to become one with the stars. It has been said only once, by me, just now. Cheers to you, celestial pearl danio! Thanks for listening to Gettin' Fishy With It! You can find our website with show notes at ⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠https://gettingfishypod.substack.com/⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠. You can find us on twitter at ⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠@gettinfishypod⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠, and on Instagram ⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠@gettingfishypod⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠. You can also find us on ⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠Facebook⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠ and ⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠LinkedIn⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠. If you want to drop us an email, you can send your complaints (or questions!) to gettingfishypod@gmail.com. Our theme music is “Best Time” by ⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠FASSOUNDS⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠. Our audio is edited by Amber Park Chiodini. Amber has her own podcast all about movies, called ⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠So What Happens Next?⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠ We very much appreciate you taking the time to listen to our forty-second episode! Please help out the podcast by subscribing on your podcast platform of choice. If you could leave us a review, that would be super helpful! If you would like to support the show, you can sign up as a paid member on our ⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠Substack⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠, or you can ⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠buy us a coffee⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠! Thanks and we'll “sea” you again in two weeks!

En la 1349-a E_elsendo el la 20.09.2024 ĉe www.pola-retradio.org: • La komenca aktualaĵo rilatas al la daŭranta batalo kontraŭ la inundo plaginta Pollandon en la lasta semajno. • Sekve en la kulturkroniko ni informas pri interesa ekspozicio de 150 eldonoj de „Sinjoro Tadeo” en Poznano; pri filmo en la dana-pola-sveda kunprodukto kandidatigita de Danio por Oskar-premio; pri arkeologiaj malkovroj ĉe la konstruplaco en Krakovo. • En la E-komunuma segmento ni informas pri aparta mesaĝo de UEA lige kun la Internacia Tago de Paco (21.09.2024) kaj pri KAEST kun la sama temo kiel la venontjara UK en Brno, Ĉeĥio. • En la rubriko „Minutoj kun literaturo” ni deklamprezentas la poeziaĵon de Lilli Promet "Aŭtuno I" en la traduko de Hilda Dresen, kiu devenas el la libro „Estona Soveta Poezio” (1977). • Muzike akompanas nin ĉi-foje Feri Floro per la kanzono „Ĝui la vivon”. La interreta foto prezentas la E-tradukon de la epopeo „Sinjoro Tadeo” de Adam Mickiewicz, kiun faris Antoni Grabowski. • En unuopaj rubrikoj de nia paĝo eblas konsulti la paralele legeblajn kaj aŭdeblajn tekstojn el niaj elsendoj, kio estas tradicio de nia Redakcio ekde 2003. La elsendo estas aŭdebla en jutubo ĉe la adreso: https://www.youtube.com/results?q=pola+retradio&sp=CAI%253D I.a. pere de jutubo, konforme al individua bezono, eblas rapidigi aŭ malrapidigi la parolritmon de la sondokumentoj, transsalti al iu serĉata fragmento de la elsendo.

Welcome to The Daily Wrap Up, a concise show dedicated to bringing you the most relevant independent news, as we see it, from the last 24 hours (3/2/24). As always, take the information discussed in the video below and research it for yourself, and come to your own conclusions. Anyone telling you what the truth is, or claiming they have the answer, is likely leading you astray, for one reason or another. Stay Vigilant. !function(r,u,m,b,l,e){r._Rumble=b,r[b]||(r[b]=function(){(r[b]._=r[b]._||[]).push(arguments);if(r[b]._.length==1){l=u.createElement(m),e=u.getElementsByTagName(m)[0],l.async=1,l.src="https://rumble.com/embedJS/u2q643"+(arguments[1].video?'.'+arguments[1].video:'')+"/?url="+encodeURIComponent(location.href)+"&args="+encodeURIComponent(JSON.stringify([].slice.apply(arguments))),e.parentNode.insertBefore(l,e)}})}(window, document, "script", "Rumble");   Rumble("play", {"video":"v4e9cxf","div":"rumble_v4e9cxf"}); Video Source Links (In Chronological Order): (20) Olga Bazova on X: "Here's everything you need to know about Google AI. https://t.co/DSUqpxJ4sg" / X (20) Robert Inlakesh on X: "YouTube just removed my channel without giving me any warning or strikes. Essentially all my content is about Palestine, including a number of documentaries I made in the occupied territories. This is what happens if you speak out against the #Gaza_Genocide https://t.co/QzXA6b3E77" / X (21) The Last American Vagabond on X: "Those actively leaning into the #TwoPartyIllusion are dishonest by default, whether they admit it to themselves or not." / X Dane Wigington Interview - Geoengineering, Nanotech & Bio-Carrier Platforms For Pathogen Dispersion X As Americans wait to hear the outcome of a federal courts ruling on water fluoridation, corporate fact checkers are attempting to confuse the public. Let's fact check the "fact checkers". Fluoride exposure causes behavioral, molecular and physiological changes in adult zebrafish (Danio rerio) and their offspring - PubMed (21) Dr Sam Soete on X: "Fluoride accumulation accumulates in pineal gland and disrupts circadian rhythms "Parental fluoride exposure disrupts the circadian rhythm, causes anxiety-like behaviour's, and decreases the levels of brain antioxidant enzymes and melatonin" Drink glass-bottled water and use… https://t.co/UWXraCtf8l" / X (21) The Last American Vagabond on X: "The illusion of their altruism is dissolving day by day. We do not live in the world many think we do. But we can.. #RulesBasedInternationalOrder?" / X GHg6LpnWwAADraB (496×607) X U.S. wants Israel to sign a letter that it won't break international law with weapons use X X (21) The Last American Vagabond on X: "The Prime Minister of Israel says “there is no genocide whatsoever” in Gaza. Instead he refers to the largely debunked narratives repeatedly spread about October 7 as the ONLY thing we should be upset about. It's okay to be outraged by this. #CeasefireNOW https://t.co/sGR9iQyoQZ" / X (104) Forensic Architecture on X: "Israel's legal team repeatedly argued that hospitals, shelters, schools, residential buildings & 'safe zones' had lost protected status & were legitimate military targets—relying on singular cases, themselves unproven, to justify widespread targeting of civilian infrastructure." / X Damning evidence of war crimes as Israeli attacks wipe out entire families in Gaza - Amnesty International An Assessment Of Visual Material Presented By The Israeli Legal Team At The Icj, 12 January 2024 ← Forensic Architecture Israel legal team's evidence at ICJ Gaza case was misleading: research group (94) Richard Grove on X: "The "land without a people" apparently had a Government.

Welcome to The Daily Wrap Up, a concise show dedicated to bringing you the most relevant independent news, as we see it, from the last 24 hours (2/29/24). As always, take the information discussed in the video below and research it for yourself, and come to your own conclusions. Anyone telling you what the truth is, or claiming they have the answer, is likely leading you astray, for one reason or another. Stay Vigilant. !function(r,u,m,b,l,e){r._Rumble=b,r[b]||(r[b]=function(){(r[b]._=r[b]._||[]).push(arguments);if(r[b]._.length==1){l=u.createElement(m),e=u.getElementsByTagName(m)[0],l.async=1,l.src="https://rumble.com/embedJS/u2q643"+(arguments[1].video?'.'+arguments[1].video:'')+"/?url="+encodeURIComponent(location.href)+"&args="+encodeURIComponent(JSON.stringify([].slice.apply(arguments))),e.parentNode.insertBefore(l,e)}})}(window, document, "script", "Rumble");   Rumble("play", {"video":"v4dwkrx","div":"rumble_v4dwkrx"}); Video Source Links (In Chronological Order): (20) Olga Bazova on X: "Here's everything you need to know about Google AI. https://t.co/DSUqpxJ4sg" / X (20) Robert Inlakesh on X: "YouTube just removed my channel without giving me any warning or strikes. Essentially all my content is about Palestine, including a number of documentaries I made in the occupied territories. This is what happens if you speak out against the #Gaza_Genocide https://t.co/QzXA6b3E77" / X (21) The Last American Vagabond on X: "Those actively leaning into the #TwoPartyIllusion are dishonest by default, whether they admit it to themselves or not." / X Dane Wigington Interview - Geoengineering, Nanotech & Bio-Carrier Platforms For Pathogen Dispersion X As Americans wait to hear the outcome of a federal courts ruling on water fluoridation, corporate fact checkers are attempting to confuse the public. Let's fact check the "fact checkers". Fluoride exposure causes behavioral, molecular and physiological changes in adult zebrafish (Danio rerio) and their offspring - PubMed (21) Dr Sam Soete on X: "Fluoride accumulation accumulates in pineal gland and disrupts circadian rhythms "Parental fluoride exposure disrupts the circadian rhythm, causes anxiety-like behaviour's, and decreases the levels of brain antioxidant enzymes and melatonin" Drink glass-bottled water and use… https://t.co/UWXraCtf8l" / X (21) The Last American Vagabond on X: "The illusion of their altruism is dissolving day by day. We do not live in the world many think we do. But we can.. #RulesBasedInternationalOrder?" / X GHg6LpnWwAADraB (496×607) X U.S. wants Israel to sign a letter that it won't break international law with weapons use X X (21) The Last American Vagabond on X: "The Prime Minister of Israel says “there is no genocide whatsoever” in Gaza. Instead he refers to the largely debunked narratives repeatedly spread about October 7 as the ONLY thing we should be upset about. It's okay to be outraged by this. #CeasefireNOW https://t.co/sGR9iQyoQZ" / X (104) Forensic Architecture on X: "Israel's legal team repeatedly argued that hospitals, shelters, schools, residential buildings & 'safe zones' had lost protected status & were legitimate military targets—relying on singular cases, themselves unproven, to justify widespread targeting of civilian infrastructure." / X Damning evidence of war crimes as Israeli attacks wipe out entire families in Gaza - Amnesty International An Assessment Of Visual Material Presented By The Israeli Legal Team At The Icj, 12 January 2024 ← Forensic Architecture Israel legal team's evidence at ICJ Gaza case was misleading: research group (94) Richard Grove on X: "The "land without a people" apparently had a Government.

KCSB News speaks with muralist Blaise Danio, who calls herself a 'nomadic artist,' about how she continues to reinvent herself as she creates her art, colorful large-scale murals, around the world. This feature is in connection with the 2024 UCSB Reads selection, "Your Brain on Art." Readings from the book can be heard on KCSB-FM 91.9 Santa Barbara on Thursdays at 5:30 p.m., paired with interviews with local artists and arts organizations.

Hoy os presento a María Jesús Molina Cimadevila, una veterinaria y científica con una sólida trayectoria de 20 años en el campo de la investigación, específicamente en el estudio de enfermedades a través de modelos animales como ratones y peces cebra. La pasión de María Jesús por la ciencia la ha llevado a formarse y trabajar en instituciones de renombre como el Instituto de Neurociencias del CSIC en Alicante. El punto de inflexión en su carrera llega cuando decide tomar una excedencia para embarcarse en un ambicioso proyecto de divulgación científica dirigido a niños. Su proyecto, denominado "Aprende con Danio": https://aprendecondanio.systeme.io/horacio se inspira en su trabajo con los peces cebra, conocidos científicamente como Danio rerio. María Jesús ideó una manera interactiva y educativa de llevar la ciencia al aula: proporciona kits que incluyen huevos de pez cebra para que los estudiantes puedan observar de cerca el proceso de desarrollo embrionario. La observación directa de la vida en desarrollo es una herramienta que María Jesús considera fundamental para sembrar la semilla de la curiosidad científica en los jóvenes estudiantes. Además de los kits, su iniciativa se apoya en una plataforma en línea que ofrece vídeos y materiales educativos, diseñados para complementar la experiencia práctica con información y contexto teórico. El proyecto no solo busca inspirar interés en las ciencias biológicas sino también proporcionar una experiencia de aprendizaje más integral y memorable. Enfrentándose a los desafíos típicos de cualquier nueva empresa, como la búsqueda de un modelo de negocio viable y sostenible, María Jesús no se desanima. Al contrario, utiliza su experiencia científica para aprender y aplicar técnicas de marketing digital. Explora el email marketing, una herramienta que le permite conectar con su audiencia y monetizar su contenido, al punto de crear un curso llamado "More Subscribers": https://www.moresubscribers.org/horacio a través del cual comparte su conocimiento con otros que buscan incursionar en este ámbito y crecer su lista de suscriptores. El episodio refleja una historia inspiradora de transición de la academia a la empresa privada, mostrando cómo las habilidades de investigación y análisis pueden ser transferibles y valiosas en el sector empresarial. María Jesús motiva a sus colegas científicos a considerar el emprendimiento como una vía viable para aplicar sus conocimientos y habilidades, sugiriendo que la capacidad de aprender y adaptarse que desarrollan en la academia puede abrir puertas en múltiples direcciones, y posiblemente con mayor retribución financiera y personal. Aquí te puedes suscribir a la newsletter para recibir material adicional de los episodios y temáticas del podcast, y otras cosas que no te esperas: https://horacio-ps.com/newsletter Aquí accedes a la comunidad de investigadores, donde podrás comentar y aprender con otros, sobre todos los temas del podcast: https://horacio-ps.com/comunidad --- Send in a voice message: https://podcasters.spotify.com/pod/show/horacio-ps/message

Maureen Rutherford, FACET Class of 2020, Chair and Associate Professor of Psychology. Her research lab studies the impact of drugs and environmental stressors on the physiological and behavioral expression of stress responses in the zebrafish (Danio rerio) model.

Danio, Danio, where art thou Danio. Dealing with housing issues so Sam is flying solo this week in a much changed episode as to what what was promised. Soz! Do you suffer from getting as bit boozy now and again? We got your back with some solid advice to the casual beer hound in some peak non-saints related chat! Sam catches up with the 2nd sweariest Fifer and Womens club captain Hannah Clark (who doesn't swear!) as we chat about the season past and next season. we also chat about 'banter with fans' and why you shouldn't have your relatives (especially grandparents) attend a match! Young Sam Porritt sticks his head through the door to chat about Premiership safety and his love for white socks to be worn in any occasion. I was very lonely this week so it would be lovely if you came and said hi! Thanks to Stjarna Apparel for their continued support.

In many episodes of the podcast, the Water Colors team likes to talk about rare, and often expensive, fish. In this episode: Ben, Amy, and Charles talk about some really cool, cheap fishes. Corrections: - In this episode, we mentioned there being 5 species in the genus Sundadanio. There are currently 8 described species in the genus. - In this episode, we mentioned that Sundadanio rasboras are native to Borneo. They are also native to Sumatra. Fishes Mentioned in This Episode: - Dwarf anchor catfish (Erethistes jerdoni) - Neon tetra (Paracheirodon innesi) - Dwarf emerald danios (Celestichthys erythromicron) - Celestial pearl danio (Celestichthys margaritatus) - Neon blue rasbora (Sundadanio goblinus) - Neon red rasbora (Sundadanio rubellus) - Neon green rasbora (Sundadanio axelrodi) - Cardinal tetra (Paracheirodon axelrodi) - Blue chameleon badis (Badis badis) - Scarlet badis (Dario dario) - Black tiger badis (Dario tigris) - Flame badis (Dario hysginon) - Dwarf green barb (Pethia phutunio) - Rosy barb (Pethia conchonius) - Brown tailed pencilfish (Nannostomus eques) - Green striped pencilfish (Nannostomus marilynae) - Beckford's pencilfish (Nannostomus beckfordi) - Sparkling gourami (Trichopsis pumila) - Giant sparkling gourami (Trichopsis schalleri) - Croaking gourami (Trichopsis vittata) - Coral red pencilfish (Nannostomus mortenthaleri) - Purple pencilfish (Nannostomus rubrocaudatus) - "Really Red" pencilfish (Nannostomus sp. "Really Red") - Zipper kuhli loach (Pangio cuneovirgata) - Kuhli loach (Pangio oblonga) - Black kuhli loach (Pangio oblonga) - Bumblebee goby (Brachygobius nunus) - Betta imbellis - Apistogramma spp. - Blue paradise fish (Macropodus opercularis) - Blind cave tetra (Astyanax mexicanus) - Buenos Aires tetra (Hyphessobrycon anisitsi) - Dwarf red rasbora (Microrasbora rubescens) - Salt and pepper cory (Corydoras habrosus) - Pygmy cory (Corydoras pygmaeus) - Black skirt tetra (Gymnocorymbus ternetzi) - Glowlight danio (Celestichthys choprae) - Giant danio (Devario aequipinnatus) - Black molly (Poecilia sphenops) - Sailfin molly (Poecilia latipinna) - Convict cichlid (Amatitlania nigrofasciata) - Rummynose tetra (Hemigrammus bleheri) - Blue platy (Xiphophorus variatus) - Parrot cichlid (Amphilophus citrinellus × Vieja melanurus) - Lemon tetra (Hyphessobrycon pulchripinnis) - Serpae tetra (Hyphessobrycon eques) - Emperor tetra (Nematobrycon palmeri) - Albino cory (Corydoras aeneus) - Green neon tetra (Paracheirodon simulans) - Clown killifish (Epiplatys annulatus) - Daisy's ricefish (Oryzias woworae) - Mekong ricefish (Oryzias mekongensis) - Songkhram ricefish (Oryzias songkhramensis) - Phoenix rasbora (Boraras merah) - Chili rasbora (Boraras brigittae) - Exclamation point rasbora (Boraras urophthalmoides) - Dwarf hatchetfish (Carnegiella myersi) - Platinum hatchetfish (Thoracocharax stellatus) - Silver hatchetfish (Gasteropelecus sternicla) - Marble hatchetfish (Carnegiella strigata) - Archerfish (Toxotes spp.) - Micropoecilia picta - Poecilia parae - Pearl danio (Danio albolineatus) - Firemouth meeki (Thorichthys meeki)

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Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2022.12.12.520151v1?rss=1 Authors: Gallas-Lopes, M., Bastos, L. M., Benvenutti, R., Panzenhagen, A. C., Piato, A., Herrmann, A. P. Abstract: The zebrafish (Danio rerio) is a model animal that is being increasingly used in neuroscience research. A decade ago, the first study on unpredictable chronic stress (UCS) in zebrafish was published, inspired by protocols established for rodents in the early 1980's. Since then, several studies have been published by different groups, in some cases with conflicting results. We conducted a systematic review to identify studies evaluating the effects of UCS in zebrafish and meta-analytically synthetized the data of neurobehavioral outcomes and relevant biomarkers. Literature searches were performed in three databases (PubMed, Scopus, and Web of Science) and a two-step screening process based on inclusion/exclusion criteria. The included studies underwent extraction of qualitative and quantitative data, as well as risk of bias assessment. Outcomes of included studies (n = 38) were grouped into anxiety/fear-related behaviour, locomotor function, social behaviour, or cortisol level domains. UCS increased anxiety/fear-related behaviour and cortisol levels while decreased locomotor function, but a significant summary effect was not observed for social behaviour. Despite including a significant number of studies, the high heterogeneity and the methodological and reporting problems evidenced in the risk of bias analysis make it difficult to assess the internal validity of most studies and the overall validity of the model. Our review thus evidences the need to conduct well-designed experiments to better evaluate the effects of UCS on the behaviour of zebrafish. Copy rights belong to original authors. Visit the link for more info Podcast created by Paper Player, LLC

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2022.11.22.517541v1?rss=1 Authors: Dotto Fontana, B., Alnassar, N., Parker, M. Abstract: Early-life adversity impacts on anxiety-related behaviors in adulthood. The effects of such adversity not only affects the animal itself, but can be passed on transgenerationally. Pervasive effects of experimentally-induced early-life stress (ELS) have been documented in adult zebrafish but it is not clear if this can be passed on via the germline. Here, we investigated the effects of ELS across three generations, by analyzing the responses of adult animals exposed to ELS in two different anxiety-related tasks, as well as in social behavior, memory, and cognition. Animals exposed to ELS (at 7 days-post-fertilization) showed a marked attenuation of specific anxiety-related behaviors (F0) when adults, and these alterations were maintained across two subsequent generations (F1 and F2). These findings suggest that zebrafish may be a useful model organism to study the transgenerational effects of ELS, and how this pertains to (for example) neuropsychiatric disorders. In addition, our data may naturally provoke questions regarding the consideration of the environment of laboratory-housed zebrafish at early developmental stages. In particular, more work may be necessary to determine how different environmental stressors could affect data variability across laboratories. Copy rights belong to original authors. Visit the link for more info Podcast created by Paper Player, LLC

Ein super Rant wartet auf euch, sonst Update über alles

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2022.11.06.515321v1?rss=1 Authors: Oda, H., Sato, Y., Kawashima, S. A., Fujiwara, Y., Mate, P., Wu, E., Vastenhouw, N. L., Kanai, M., Kimura, H. Abstract: In the cytoplasm, filamentous actin (F-actin) plays a critical role in cell regulation, including cell migration, stress fiber formation, and cytokinesis. Recent studies have shown that actin filaments that form in the nucleus are associated with diverse functions. Here, using live imaging of an F-actin-specific probe, superfolder GFP-tagged utrophin (UtrCH-sfGFP), we demonstrated the dynamics of nuclear actin in zebrafish (Danio rerio) embryos. In early zebrafish embryos up to around the high stage, UtrCH-sfGFP increasingly accumulated in nuclei during the interphase and reached a peak during the prophase. After nuclear envelope breakdown (NEBD), patches of UtrCH-sfGFP remained in the vicinity of condensing chromosomes during the prometaphase to metaphase. When zygotic transcription was inhibited by injecting -amanitin, the nuclear accumulation of UtrCH-sfGFP was still observed at the sphere and dome stages, suggesting that zygotic transcription may induce a decrease in nuclear F-actin. The accumulation of F-actin in nuclei may contribute to proper mitotic progression of large cells with rapid cell cycles in zebrafish early embryos, by assisting in NEBD, chromosome congression, and/or spindle assembly. Copy rights belong to original authors. Visit the link for more info Podcast created by Paper Player, LLC

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2022.10.31.514532v1?rss=1 Authors: Aljiboury, A. A., Ingram, E., Krishnan, N., Ononiwu, F., Pal, D., Manikas, J., Taveras, C., Hall, N. A., Da Silva, J., Freshour, J., Hehnly, H. Abstract: An essential process for cilia formation during epithelialization is the movement of the centrosome to dock with the cell's nascent apical membrane. Our study examined centrosome positioning during the development of Danio rerio's left-right organizer (Kupffer's Vesicle, KV). We found that when KV mesenchymal-like cells transition into epithelial cells that are organizing into a rosette-like structure, KV cells move their centrosomes from random intracellular positions to the forming apical membrane in a Rab11 and Rab35 dependent manner. During this process, centrosomes construct cilia intracellularly that associated with Myo-Va while the centrosomes repositioned towards the rosette center. Once the centrosomes with associated cilia reach the rosette center, the intracellular cilia recruit Arl13b until they extend into the forming lumen. This process begins when the lumen reaches an area of approximately 300 m2. Using optogenetic and depletion strategies, we identified that the small GTPases, Rab11 and Rab35, regulate not only cilia formation, but lumenogenesis, whereas Rab8 was primarily involved in regulating cilia length. These studies substantiate both conserved and unique roles for Rab11, Rab35, and Rab8 function in cilia formation during lumenogenesis. Copy rights belong to original authors. Visit the link for more info Podcast created by Paper Player, LLC

A read through and discussion of Calisto and Melebea, from the press of John Rastell Another in our occasional series of cross overs with our YouTube channel, this is a Second Look read through and discussion of Calisto and Melebea, coming from the printing press of John Rastell around 1525. It's an adaptation of a Spanish book/play Celestina, later published in English as The Spanish Bawd. This is our first take, recorded in 2021, returning to the play after a year or so, as prep for a more detailed run - the video of which can be watched here. https://www.youtube.com/watch?v=GlkGUCoxIwQ With Gregory Musson as Danio; Eric Karoulla as Calisto; Rachael Nicole as Melebea; Lynsey Beauchamp as Celestina; Alex Scott Fairley as Parmeno; Alexandra Kataigida as Sempronio. The host was Robert Crighton. The character of Celestina has many parallels in Dame Sirith and other texts. We haven't released our work on Dame Sirith at time of release, but the mentioned work on Interludium de Clerico et Puella is on the podcast now - https://audioboom.com/posts/7224328-interludium-de-clerico-et-puella-fragment All the videos on Calisto and Melebea (1st, 2nd Looks and character workshop) can be viewed via this handy playlist - https://www.youtube.com/watch?v=jWm5fLrrEqs&list=PLflmEwgdfKoLYUZxhyx34AJpuYvEtfGxp To learn more about John Rastell, go to our Discussion episode on the man and his writing - https://audioboom.com/posts/7013153-discussing-john-rastell The Beyond Shakespeare Podcast is supported by its patrons – become a patron and you get to choose the plays we work on next. Go to www.patreon.com/beyondshakespeare - or if you'd like to buy us a coffee at ko-fi https://ko-fi.com/beyondshakespeare - or if you want to give us some feedback, email us at admin@beyondshakespeare.org, follow us on Twitter, Facebook & Instagram @BeyondShakes or go to our website: https://beyondshakespeare.org You can also subscribe to our YouTube channel where (most of) our exploring sessions live - https://www.youtube.com/channel/UCLa4pXxGZFwTX4QSaB5XNdQ The Beyond Shakespeare Podcast is hosted and produced by Robert Crighton

Transgenic zebrafish engineered to glow various colors have escaped likely illegal aquariums in Brazil into natural waters. The fish are now observed to be mating in the wild and growing in numbers. ThePrint's Sandhya Ramesh explains more. Brought to you by  @Kia India  ----more----Subscribe to the Pure Science Telegram Channel https://t.me/PureScienceWithSandhyaRamesh----more----Supplementary reading: Magalhães, et al., 2022, The fluorescent introduction has begun in the southern hemisphere: presence and life-history strategies of the transgenic zebrafish Danio rerio (Cypriniformes: Danionidae) in Brazil, Studies on Neotropical Fauna and Environment,: https://www.tandfonline.com/doi/full/10.1080/01650521.2021.2024054

In today's video, I'll be revealing 7 reasons why modern faces are shrinking, and what this means for your health and well-being. ______________________________________________________________ Energize Your Day Starter Guide: 5 simple steps to wake up refreshed and ready to go http://energizeyourdaytoday.com Sleep Interrupted: A physician reveals the #1 reason why so many of us are sick and tired. https://amzn.to/3ssXebE Consider the Fork: A History of How We Cook And Eat https://amzn.to/3Fl4g5J A History of Infant Feeding https://www.ncbi.nlm.nih.gov/pmc/arti... Fluorosilicic Acid Material Safety Data Sheet https://www.dudesandbeer.com/KNOWLEDG... The effects of pregnancy and fluoride on orthodontic tooth movements in rats https://academic.oup.com/ejo/article-... Effect of sodium fluoride and sodium pyruvate on palatal development in vitro https://onlinelibrary.wiley.com/doi/a... The Grand Rapids Fluoride Study https://hartkeisonline.com/2011/01/12... Modulation of estrogen causes disruption of craniofacial chondrogenesis in Danio rerio https://www.sciencedirect.com/science... Short exposure to glyphosate induces locomotor, craniofacial, and bone disorders in zebrafish (Danio rerio) embryos https://www.sciencedirect.com/science... Developmental alterations, teratogenic effects, and oxidative disruption induced by ibuprofen, aluminum, and their binary mixture on Danio rerio https://www.sciencedirect.com/science...

Scientists have also studied the nighttime rituals of another autism model: zebrafish (Danio rerio). Gene-editing techniques enable scientists to create zebrafish with autism-linked mutations, and then they can easily assess how the mutations effect behavior.

On this week's The Sci-Files, your hosts Chelsie and Danny interview Cameron Bennett. Cameron is a recent graduate from Lyman Briggs College who majored in genomics and molecular genetics and human biology. Since her sophomore year at MSU, Cameron has done research with the Braasch lab in the Department of Integrative Biology studying the function of duplicated endothelin genes unique to a group of fish called teleosts. The endothelin gene family she studies has an important role in controlling what type of cells that neural crest cells become during early development. Neural crest cells are special as they are unique to vertebrates like fish and humans and they can become many tissues, including heart, pigment cells, enteric (gut) nerve cells, and parts of the skeleton making up the head and face. Over the course of evolution, vertebrates underwent two whole-genome duplication events. The endothelin system consists of three proteins in most vertebrates: Edn1, Edn2, and Edn3. In contrast, following a third whole-genome duplication, the endothelin system in teleost fish consists of six proteins: Edn1, Edn2a, Edn2b, Edn3a, Edn3b, and Edn4. Not only are there extra copies of Edn2 and Edn3 in this fish group, but the presence of an additional unique protein, Edn4. Currently, the function of most Edn proteins remains a mystery. Cameron's research project aims to better understand the developmental role of the Edn proteins in the teleost fish zebrafish, Danio rerio. She has been working on this by developing lines of zebrafish with combinations of mutations across the endothelin gene family using the CRISPR-Cas9 genome editing technique. If you're interested in talking about your MSU research on the radio or nominating a student, please email Chelsie and Danny at scifiles@impact89fm.org. Check The Sci-Files out on Twitter, Facebook, Instagram, LinkedIn, and YouTube! 

Even the most common, captive-bred, pervasive fishes in the aquarium hobby came from somewhere “out there” once, right? Like our friend the Zebra Danio- staple of the hobby for a century. Let’s see what we can find when we delve into scientific, rather than hobby treatises on this fish. Let’s go “off the grid” with The Zebra Danio!

Interesante conversación sobre el Proyecto"Cerro Punta Negra en el Valle de Uco.-

You're listening to the April episode of 3 Minute 3Rs.The papers behind the pod:1. Identification of individual zebrafish (Danio rerio): a refined protocol for VIE tagging whist considering animal welfare and the principles of the 3RS. Animals 11, 616 (2021) https://doi.org/10.3390/ani110306162. Enrichment for laboratory zebrafish – a review of the evidence and challenges. Animals 11, 698 (2021) https://doi.org/10.3390/ani110306983. Research relevant background lesions and conditions: ferrets, dogs, swine, sheep, and goats. ILAR J ilab005 (2021) https://doi.org/10.1093/ilar/ilab0054. *Bonus* Research-relevant background lesions and conditions in common avian and aquatic species. ILAR J ilab008 (2021) https://doi.org/10.1093/ilar/ilab008Transcript: It's the 3rd Thursday of April and you're listening to 3 Minute 3Rs, your monthly recap of efforts to replace, reduce, and refine the use of animals in research. This month we've got some zebrafish refinements, plus a refresher on some basics for a bunch of different animals. But first, ID please. [NC3Rs]If you've got a tank full of fish that look identical, how do you make sure you can tell them apart? The zebrafish research community have tried various tagging methods, but until now there hasn't been a refined and standardized method that can be used on a large scale.In a new paper in Animals, Anita Rácz and colleagues describe such a method for tagging zebrafish using visible implant elastomer, or VIE. This method is widely used for marking large fish and other aquatic organisms and involves injecting a small amount of coloured elastomer under the fish's skin.As well as explaining their refined protocol, including appropriate anaesthesia and analgesia, Rácz and colleagues compare it to a previous protocol and show that it offers significant welfare improvements, including a ten-fold reduction in mortality following the tagging procedure. With zebrafish being used in thousands of labs around the world, particularly in genetic research, this work is bound to make a big splash. While we're thinking about zebrafish, have you thought about their tanks look like lately? [NA3RsC] Zebrafish have rapidly become one of the most common research animal species with over 5 million fish used worldwide. Currently, most of these fish are housed in barren tanks with no environmental enrichment.But in a recent review in Animals, the RSPCA Science Department describes current evidence supporting zebrafish enrichment. Its provision improves zebrafish welfare, behavior, and physiology, which can lead to better science. One well-supported and fairly easily implemented enrichment is using an image of gravel on the bottom of tanks. The paper also discuss evidence for social housing, plastic plants, live food, and more. To learn more about the current evidence for zebrafish enrichment, read the full paper online. [LA] And finally, a double feature. A lot of different animals turn up in research labs beyond rodents. But regardless of what species you are using, you need to know what's normal and what's not for a given animal in order to accurately interpret any experimental results. How well do you know an animals' anatomy and how it differs from the humans they are intended to model? Is that lesion intentional, or a spontaneous... See acast.com/privacy for privacy and opt-out information.

En este episodio, nos sumergimos en la tesis doctoral de: -Beatriz Carnicero, PhD en Ciencias, manejo de Recursos Acuáticos Renovables (MaReA) en el Centro de Biotecnología de la Universidad de Concepción en Chile. Hablamos de modelos experimentales con “Danio recio”, este pececillo que comparte un 70% de nuestro genoma. Y Beatriz también nos cuenta su enrevesado camino por la investigación hasta llegar a la Universidad de Chile para hacer su doctorado. ¿Qué diferencias hay entre Chile y España en relación a los predocs? ¿Cómo es el mundo de la investigación en este país sudamericano? ¡No os perdáis lo que nos cuenta Beatriz sobre su experiencia personal buscando su camino en la investigación! Y no os olvidéis de seguirnos en nuestras RRSS para que sigamos contándoos experiencias de PhDs por todo el mundo: Instagram: @phdtalks_ Twitter: @PhDtalks___ Email: phdtalks.es@gmail.com Abrazos, compañeros

Conversamos con don Danio Ramonda de la Camara de Comercio del Valle de Uco; realizando un balance de estos primeros 15 días de enero 2021.-

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2020.11.16.385930v1?rss=1 Authors: Mason, D., Zajitschek, S., Anwer, H., O'Dea, R., Hesselson, D., Nakagawa, S. Abstract: Aversive learning, avoiding certain situations based on negative experiences, can profoundly increase fitness in animal species. The extent to which this cognitive mechanism could evolve depends upon individual differences in aversive learning being stable through time, and heritable across generations, yet no published study has quantified the stability of individual differences in aversive learning using the repeatability statistic, R (also known as the intra-class correlation). We assessed the repeatability of aversive learning by conditioning approximately 100 zebrafish (Danio rerio) to avoid a colour cue associated with a mild electric shock. Across eight different colour conditions zebrafish did not show consistent individual differences in aversive learning (R = 0.04). Within conditions, when zebrafish were twice conditioned to the same colour, blue conditioning was more repeatable than green conditioning (R = 0.15 and R = 0.02). In contrast to the low repeatability estimates for aversive learning, zebrafish showed moderately consistent individual differences in colour preference during the baseline period (i.e. prior to aversive conditioning; R {approx} 0.45). Overall, aversive learning responses of zebrafish were weak and variable (difference in time spent near the aversive cue

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2020.11.13.381954v1?rss=1 Authors: Parkinson, L., Stawicki, T. M. Abstract: Multiple cilia-associated genes have been shown to affect hair cells in zebrafish ()Danio rerio), including the human deafness gene dcdc2 , the radial spoke gene rsph9 , and multiple intraflagellar transport (IFT) and transition zone genes. Recently a zebrafish alms1 mutant was generated. The ALMS1 gene is the gene mutated in the ciliopathy Alstrom Syndrome a disease that causes hearing loss among other symptoms. The hearing loss seen in Alstrom Syndrome may be due in part to hair cell defects as Alms1 mutant mice show stereocilia polarity defects and a loss of hair cells. Hair cell loss is also seen in postmortem analysis of Alstrom patients. The zebrafish alms1 mutant has metabolic defects similar to those seen in Alstrom syndrome and Alms1 mutant mice. We wished to investigate if it also had hair cell defects. We, however, failed to find any hair cell related phenotypes in alms1 mutant zebrafish. They had normal lateral line hair cell numbers as both larvae and adults and normal kinocilia formation. They also showed grossly normal swimming behavior, response to vibrational stimuli, and FM1-43 loading. Mutants also showed a normal degree of sensitivity to both short-term neomycin and long-term gentamicin treatment. These results indicate that cilia-associated genes differentially affect different hair cell types. Copy rights belong to original authors. Visit the link for more info

Interesante conversación con don Danio Ramonda de la CIAT de Tunuyán - Valle de Uco - Mendoza; sobre la Zonificación de la Reserva Manzano - ...Piuquenes.

Conversamos con Danio Ramonda del evento Binacional "JORNADAS DE INTEGRACIÓN HISTÓRICA CULTURAL CHILENO - ARGENTINO"

Conversamos con el prosecretario de la Cámara de Comercio, Industria, Agricultura y Turismo de Tunuyán - Valle de Uco - Mendoza; sobre el desafío del Paso Fronterizo Portillo - Piuquenes.

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2020.09.21.305979v1?rss=1 Authors: Garcia - Jaramillo, M., Beaver, L. M., Truong, L., Axton, E. R., Keller, R. M., Prater, M. C., Magnusson, K. R., Tanguay, R. L., Stevens, J. F., Hord, N. G. Abstract: Introduction Dietary nitrate lowers blood pressure and improves athletic performance in humans, yet data supporting observations that it may increase cerebral blood flow and improve cognitive performance are mixed. Here we tested the hypothesis that nitrate and nitrite treatment would improve indicators of learning and cognitive performance in a zebrafish ( Danio rerio ) model. We also explored the extent to which nitrate and nitrite treatment affected the brain metabolome in order to understand how nitrate and nitrite supplementation may affect indices of cognitive function. Methods Fish were exposed to sodium nitrate (606.9 mg/L), sodium nitrite (19.5 mg/L), or control water for 2-4 weeks and free swim, startle response, innate predator avoidance, social cohesion, and shuttle box assays were performed. Results Nitrate and nitrite treatment did not change fish weight, length, predator avoidance, or distance and velocity traveled in an unstressed environment. Nitrate- and nitrite-treated fish initially experienced more negative reinforcement and increased time to decision in the shuttle box assay, which is consistent with a decrease in associative learning or executive function however, over multiple trials, all treatment groups demonstrated behaviors associated with learning. Nitrate and nitrite treatment significantly increased anxiety-like behavior but did not alter epinephrine, norepinephrine or dopamine levels. Targeted LC-MS/MS analysis revealed no significant increase in brain nitrate or nitrite concentrations with treatment. An untargeted metabolomics analysis found 47 metabolites whose abundance was significantly altered in the brain with nitrate and nitrite treatment including an 18-19% reduction in the neurotransmitter {gamma}-aminobutyric acid (GABA), and 17-22% reduction in its precursor, glutamine, which may contribute to the increased anxiety-like behavior. Conclusion Nitrate and nitrite treatment did not adversely affect multiple parameters of zebrafish health but was associated with mild anxiety-like behavior, changes in the brain metabolome, and caused a short-term decrease in executive function or associative learning. Copy rights belong to original authors. Visit the link for more info

Danio is a London based hip hop producer who has worked with the likes of Kari Faux, twst and SBK. He also fronts the experimental rock band Husky Loops who released their superb debut record "I CAN'T EVEN SPEAK ENGLISH" last August and have supported the likes of David Byrne. We talk all things Husky Loops, innocence, design, brands, personality, his passion for fashion, their live show, honesty, Kari Faux, twst, outsiders, risks, Song Confessional, the darker sides of personalities, phones, Pet Sounds, Elephant, closure with songs and Gucci sunglasses. Theme Music: "Breakfast Burger" by Snack Villain See acast.com/privacy for privacy and opt-out information.

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2020.06.19.160804v1?rss=1 Authors: Guggiana Nilo, D. A., Riegler, C., Hübener, M., Engert, F. Abstract: Larval zebrafish (Danio rerio) are an ideal organism to study color vision, as their eye possesses four types of cone photoreceptors, covering most of the visible range and into the UV [1,2]. Additionally, their entire eye and nervous system are accessible to imaging, given they are naturally transparent [3-5]. Relying on this advantage, recent research has found that, through a set of color specific horizontal, bipolar and retinal ganglion cells (RGCs) [6-8], the eye then relays tetrachromatic information to several retino-recipient areas (RAs) [9,10]. The main RA is the optic tectum, receiving 97% of the RGC axons via the neuropil mass termed Arborization Field 10 (AF10) [11,12]. In this work, we aim to understand the processing of color signals at the interface between RGCs and their targets in the brain. We used 2-photon calcium imaging to separately measure the responses of RGCs and neurons in the dorsal brain to stimulation with four different colors in awake animals. We find that color information is widespread throughout the larval brain, with a large variety of color responses among RGCs, and an even greater diversity in their targets. Specific combinations of response types are localized to specific nuclei, but we observe no single color processing structure. In the main interface in this pathway, the connection between Arborization Field 10 and the tectum, we observe key elements of color processing such as enhanced signal decorrelation and improved decoding [13,14]. Finally, when presenting a richer set of stimuli, we identify parallel processing of color, motion and luminance information in the same cells/terminals, evidence of a rich color vision machinery in this small vertebrate brain. Copy rights belong to original authors. Visit the link for more info

Game 1 of the 2015 World Series arrived on the same day as the death of Edinson Volquez’s father, Danio. The news reached Royals general manager Dayton Moore, who relayed it to manager Ned Yost, before Volquez knew. With game time approaching and Volquez slated to take the ball for the Royals, a decision was needed. Would the team let Volquez know? The team made the call in consultation with the Volquez family. Edinson wouldn’t be told. He pitched six solid innings and the Royals went on to win in 14 innings. On today’s SportsBeat KC, Yost recounts the hours before the game and what communication was happening behind the scenes with Star columnist Vahe Gregorian and host Blair Kerkhoff. Story links: In postseason of epic Royals comebacks, none were more poignant than Edinson Volquez’s Relive the crowing of the 2015 Royals: Here’s how they won it in Game 5 Learn more about your ad choices. Visit megaphone.fm/adchoices

On MIA Radio this week, MIA's Zenobia Morrill interviews Dr. Vance Trudeau, a professor at the University of Ottawa in Canada. Dr. Trudeau describes a recent study he conducted, alongside a team of researchers, led by Dr. Marilyn Vera-Chang, that has implications for understanding of the long-term impact of antidepressant drug exposure (see MIA report). The study, titled Transgenerational hypocortisolism and behavioral disruption are induced by the antidepressant fluoxetine in male zebrafish Danio rerio linked antidepressant exposure to decreased coping behaviors in zebrafish that lasted several generations. Dr. Trudeau is the research chair in neuroendocrinology at the University of Ottawa, where he studies how the brain regulates hormonal activity in fish and frogs. Such analyses offer important insights into the effects of environmental exposures on human health because these hormonal systems are shared across species. © Mad in America 2019

Gościem 18 odcinka jest dr Artur Gołaś z katowickiej AWF. Trener przygotowania motorycznego, który specjalizuje się w zakresie struktury wewnętrznej ruchu, siły mięśniowej oraz przygotowania motorycznego. Jak sam mówi łączy teorię z praktyką, dlatego oprócz pracy na uczelni pracuje z piłkarzami zespołu Zagłębie Sosnowiec oraz sportowcami różnych dyscyplin sportowych.Z Arturem rozmawiamy o tym, że wyniki sportowe nie są efektem tylko pracy na treningu ale wynikają z tego, jaki jesteś zawodniku, trenerze czy człowieku, który lubi aktywność fizyczną, lubi biegać, chodzić na siłownię czy uprawiać inną aktywność na co dzień. Że jest to efekt tego jak podchodzisz do codziennych , zwykłych spraw takich jak odżywianie, sen, tryb życia czyli jak ważna jest ŚWIADOMOŚĆ . To także a może głównie relacja z samym sobą: jaki jesteś, jak sam siebie postrzegasz. To suma tego co robisz w tej chwili i co robiłeś jak byłeś dzieckiem, jakie wzorce ruchowe ze sobą nosisz przez całe życie, jak pracowałeś na wf-ie w szkole czy twój nauczyciel wf zwracał ci uwagę jak robisz przysiad albo skłon. Czy potrafisz prawidłowo podnieść z ziemi np. wiadro z wodą. To są wzorce ruchowe, o których dużo rozmawiamy. Nasza rozmowa dotyczy głównie tego co przed zawodami - jak przygotować swoje ciało, jak nastawić swój umysł do wysiłku. Jak trenować żeby nie zrobić sobie krzywdy? Jak kształtować dobre zdrowe nawyki już od najmłodszych lat ( Danio nie jest na głoda jak mówi mój gość :) Co warto a czego nie warto żeby osiągnąć sukces czyli to co sobie zakładasz?Serdecznie zapraszam do wysłuchania odcinka. Będzie fajnie jeśli zostawisz recenzję lub ocenę tego odcinka , jeśli udostępnisz u siebie lub polecisz go znajomym. Im więcej ludzi będzie miało okazję wysłuchać tego podcastu tym lepsze i zdrowsze będziemy mieć społeczeństwo. To dobrze, nie?Jeśli chciałbyś się skontaktować z moim gościem - Arturem Gołasiem to znajdziesz go na AWF w Katowicach w Pracowni Siły i Mocy Mięśniowej lun na FBhttps://www.facebook.com/profile.php?id=100000539680003https://awf.katowice.pl/pracownicy/artur-golas

Gościem 18 odcinka jest dr Artur Gołaś z katowickiej AWF. Trener przygotowania motorycznego, który specjalizuje się w zakresie struktury wewnętrznej ruchu, siły mięśniowej oraz przygotowania motorycznego. Jak sam mówi łączy teorię z praktyką, dlatego oprócz pracy na uczelni pracuje z piłkarzami zespołu Zagłębie Sosnowiec oraz sportowcami różnych dyscyplin sportowych.Z Arturem rozmawiamy o tym, że wyniki sportowe nie są efektem tylko pracy na treningu ale wynikają z tego, jaki jesteś zawodniku, trenerze czy człowieku, który lubi aktywność fizyczną, lubi biegać, chodzić na siłownię czy uprawiać inną aktywność na co dzień. Że jest to efekt tego jak podchodzisz do codziennych , zwykłych spraw takich jak odżywianie, sen, tryb życia czyli jak ważna jest ŚWIADOMOŚĆ . To także a może głównie relacja z samym sobą: jaki jesteś, jak sam siebie postrzegasz. To suma tego co robisz w tej chwili i co robiłeś jak byłeś dzieckiem, jakie wzorce ruchowe ze sobą nosisz przez całe życie, jak pracowałeś na wf-ie w szkole czy twój nauczyciel wf zwracał ci uwagę jak robisz przysiad albo skłon. Czy potrafisz prawidłowo podnieść z ziemi np. wiadro z wodą. To są wzorce ruchowe, o których dużo rozmawiamy. Nasza rozmowa dotyczy głównie tego co przed zawodami - jak przygotować swoje ciało, jak nastawić swój umysł do wysiłku. Jak trenować żeby nie zrobić sobie krzywdy? Jak kształtować dobre zdrowe nawyki już od najmłodszych lat ( Danio nie jest na głoda jak mówi mój gość :) Co warto a czego nie warto żeby osiągnąć sukces czyli to co sobie zakładasz?Serdecznie zapraszam do wysłuchania odcinka. Będzie fajnie jeśli zostawisz recenzję lub ocenę tego odcinka , jeśli udostępnisz u siebie lub polecisz go znajomym. Im więcej ludzi będzie miało okazję wysłuchać tego podcastu tym lepsze i zdrowsze będziemy mieć społeczeństwo. To dobrze, nie?Jeśli chciałbyś się skontaktować z moim gościem - Arturem Gołasiem to znajdziesz go na AWF w Katowicach w Pracowni Siły i Mocy Mięśniowej lun na FBhttps://www.facebook.com/profile.php?id=100000539680003https://awf.katowice.pl/pracownicy/artur-golas

This is the January episode of 3-Minute 3Rs, brought to you by the North American 3Rs Collaborative (www.na3rsc.org, the NC3Rs (www.nc3rs.org.uk), and Lab Animal (www.nature.com/laban) The papers behind the pod: 1. An Atypical Parvovirus Drives Chronic Tubulointerstitial Nephropathy and Kidney Fibrosis https://bit.ly/2FA6dPA 2. Remote Welfare Monitoring of Rodents Using Thermal Imaging https://bit.ly/2suGlgm 3. No experimental evidence of stress-induced hyperthermia in zebrafish (Danio rerio)https://bit.ly/2Db8m2P [NA3RsC] Chronic kidney disease results from fibrosis of the kidney parenchyma due to a variety of inciting factors. Existing models of tubulointerstitial nephropathy and fibrosis involves administration of nephrotoxic compounds or surgical interventions, but these models often fail to recapitulate the chronic, insidious nature of fibrotic disease. In a recent publication from the October 4, 2018 issue of Cell, Ben Roediger and his colleagues at the University of Sidney Australia observed a high incidence of kidney disease in 3 separate colonies of immunodeficient mice; disease was characterized by enlarged nuclei in tubular epithelial cells in addition to intranuclear inclusions and marginated chromatin. RNA sequencing identified a complete novel viral genome that was given the name ‘mouse kidney parvovirus.' Resulting pathology included extensive fibrotic changes to the kidneys with significantly reduced renal mass. Virus was absent in mouse colonies free of kidney disease, transmissible following co-housing and infected mice exhibited similar transcriptional changes to humans with fibrotic disease. Ultimately, mouse kidney parvovirus may represent a new tool for understanding viral nephropathy as well as the fibrotic processes and pathogenesis of chronic kidney disease. [NC3RS] To assess the well-being of an animal, it is useful to be able to continuously monitor their vital signs, such as heart rate and breathing rate, and the amount they move. Vital signs can be assessed by implanting a telemetry sensor or using electrocardiography. However, these methods can lead to unnecessary discomfort and stress for the animal and can compromise their welfare. A recent study by Pereira et al. addresses these issues by using thermal imaging to monitor animal welfare. Thermal imaging is a remote and passive monitoring technique that can continuously record the heat emitted from the animal's body. Using this technology, the researchers were able to remotely monitor breathing rate of anaesthetised rats and mice, and the position and velocity of active rodents during open field tests. This promising technique has the potential to refine current monitoring practice and improve animal welfare. Follow the link in the description to find out more. [LA] When endothermic animals, like ourselves or our laboratory mammals, get stressed, we often get hot. Literally. But not all animals control their internal body temperature. A 2015 paper published in the Proceedings of the Royal Society B indicated that zebrafish stressed out from temporary confinement will deliberately move to a warmer area of their tank in order to raise their body temperature. This suggests that they too exhibit stress-induced hyperthermia. But questions have lingered about whether the fish were actively seeking heat or rather, just moving away from the chamber where they had been confined. Writing in the Journal of Experimental Biology, researchers from the University of St. Andrews repeated that experiment, but included control tanks without a temperature gradient. They saw no discernible effect. They also developed a second tank set up - in that case, stressed fish actually spent a little less time in warmer water. Don't neglect zebrafish stress, the authors stress, but their results suggest that the fish don't experience stress-induced hyperthermia in the same as other animals. See acast.com/privacy for privacy and opt-out information.

In this month's 3-minute 3R's, we're talking temperature, environmental enrichment to improve stress responses in zebrafish, and better ways to handle mice. The papers behind the pod: Mice Housed at Elevated Vivarium Temperatures Display Enhanced T-cell Response and Survival to Francisella tularensis: http://www.ingentaconnect.com/content/aalas/cm/2017/00000067/00000006/art00004 Heat or Insulation: Behavioral Titration of Mouse Preference for Warmth or Access to a Nest: http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0032799 International survey on the use and welfare of zebrafish Danio rerio in research: http://onlinelibrary.wiley.com/doi/10.1111/jfb.13278/abstract Environmental enrichment modulates the response to chronic stress in zebrafish: http://jeb.biologists.org/content/early/2018/01/09/jeb.176735 Handling method alters the hedonic value of reward in laboratory mice: https://www.nature.com/articles/s41598-018-20716-3 The pod was produced this month with contributions from Lab Animal (www.nature.com/laban), the NC3Rs in the UK (www.nc3rs.org.uk), and the North American 3Rs Collaborative (www.na3rsc.org). See acast.com/privacy for privacy and opt-out information.

The sandwich generation is a generation of people who care for their aging parents while supporting their own children.There are many emotions that go along with being a sandwich generation caregiver such as physical & emotional stress, financial burden, and burnout.  For many individuals with senior parents who live in their own homes, mobility and safety is a constant worry. When someone you love is receiving care, you want to stay informed, whether you live in the same town or thousands of miles away. With Danio Diary™, you can be sure you get real-time updates from the people providing care to your loved one. Whether you’re helping to coordinate care or simply want the peace of mind that comes from knowing that you’re getting information when you need it, Danio is there for you.  Listen in to this evening's Podcast as I talk to Anthony Wright, Marketing Director for the Danio Diary™ a free app that will give you "Peace of Mind in Real Time" about our loved ones' care.  

The microbiome of hibernating bears, and zebrafish as a model for bacterial sepsis feature in this animal-centric episode of TWiM hosted by Vincent, Michael, and Michele. Hosts: Vincent Racaniello, Michele Swanson, and Michael Schmidt. Subscribe to TWiM (free) on iTunes, Stitcher, RSS, or by email. You can also listen on your mobile device with the Microbeworld app. Links for this episode Microbiome of hibernating brown bear and energy metabolism (Cell Rep) Ten animals that hibernate (Cons Inst) Zebrafish model of sepsis (mSphere) Image credit Letters read on TWiM 126 This episode is sponsored by ASM Agar Art Contest and ASM Microbe 2016 Send your microbiology questions and comments (email or mp3 file) to twim@twiv.tv, or call them in to 908-312-0760. You can also post articles that you would like us to discuss at microbeworld.org and tag them with twim.  

The phylotypic stage, as part of the embryonic period, is the stage where embryos of different species of a phylum show a high degree of similarity. Johann Friedrich Meckel, Karl Ernst von Baer and Ernst Haeckel already described it for vertebrates in the 19th century. They observed that vertebrate embryos pass through a period of morphological similarity. Since then, scientists have researched the field of the phylotypic stage and it was subject of many controversial discussions. The name “phylotypic stage” was coined by Klaus Sander in 1983 and describes not only the stage of the highest similarity but also the stage, typical (characteristic) for a phylum. The following study examines the phylotypic stage of zebrafish (Danio rerio). Looking at different conserving mechanisms like internal constrains and stabilizing selection, different hypothesis and concepts by several researchers were tested. To test if the phylotypic stage is accessible to selection (although it generally is considered a conserved evolutionary stage) I have studied patterns of variation during embryogenesis. I have looked at the phenotypic variance and the number of significant correlations among embryonic traits and described the phylotypic stage as a period characterized by a high number of internal correlations and declining phenotypic variance. Then, I tested if changes in the raising conditions could elicit phenotypic changes. Therefore, zebrafish embryos have been raised under different experimental conditions to see if developmental plasticity can be induced during the early developmental period and if clearly defined modules can be identified. Eggs of zebrafish were raised in: (1) different temperatures; (2) different salinities; and (3) different levels of oxygen concentration. Up to 14 characters of individual embryos were measured during early development, encompassing the phylotypic stage. In particular I found a considerable degree of heterochrony and modularity. Embryos grew slower at lower temperatures and lower oxygen levels. Plasticity was detected in the overall size of the embryo and the size of somites in the oxygen and temperature experiment. The development of the eye and otic vesicle was shifted to a later x stage under severe hypoxia. Thus, eye and otic vesicle could be identified as modules, which can be dissociated from other characters of the developing embryo (heterochrony). Changes in raising condition affect early development of the zebrafish on three levels: (1) developmental rate (2) size and shape, and (3) dissociation of modules. Thus, plasticity and modularity are effective during early embryonic development. Finally I studied the heritability of embryonic traits to examine how inheritance contributes to the stabilization of the phylotypic stage in variable environments. Following the heritabilities of certain traits reveals that the phylotypic stage is not characterized by a certain pattern of decreased heritability and thus decreased additive genetic variance. The results suggest that the phylotypic stage of zebrafish is constrained by multiple internal correlations when embryos are developing in standard conditions. However, under marginal developmental conditions so far ineffective modules become effective and buffer the embryo against disruptive effects of the environment. Patterns of family resemblance are present, indicating an inherited genetic portion of the phylotypic stage. However, under strong environmental influence it is dominated by variation associated with phenotypic plasticity. My general conclusion is that the phylotypic stage is not established because additive genetic variance is exhausted during the early period of vertebrate development but that it is under environmental and genetic influence, thus is accessible to selection. Internal constraints could be identified to stabilize morphology during the phylotypic stage, but a certain degree of phenotypic variation can be observed.

An interview with author Elizabeth Kirke. She's a veteran of Utopia Con, has been there since year one, and is the author of the young adult series More Than Magic, which includes: Semester Aboard, Snow Bound, Soul Choice, and Danio's Prelude. Also in her YA arsenal is the Curse Collectors series, which kicked off last year with Carved in Cherry. You can find Elizabeth on the web at elizabeth-kirke.blogspot.com. Not only is she an author, she also does formatting for print on demand titles. Find Jo here: Jo Michaels Blog Find Ren here: A Little Bit of R&R

Mutations in the gene encoding the E3 ubiquitin ligase parkin (PARK2) are responsible for the majority of autosomal recessive parkinsonism. Similarly to other knockout mouse models of PD-associated genes, parkin knockout mice do not show a substantial neuropathological or behavioral phenotype, while loss of parkin in Drosophila melanogaster leads to a severe phenotype, including reduced lifespan, apoptotic flight muscle degeneration and male sterility. In order to study the function of parkin in more detail and to address possible differences in its role in different species, we chose Danio rerio as a different vertebrate model system.

Vorangegangene Studien zeigten, dass das Prion-Protein (PrP) an der ER-Membran in verschiedenen topologischen Isoformen synthetisiert wird und teilweise sogar im Zytosol vorliegen kann. Sowohl die ER-Signalsequenz als auch die hydrophobe Domäne von PrP wurden dabei als Domänen identifiziert, die eine Rolle in der Translokation spielen. Die hier durchgeführte Analyse des ER-Imports von PrP und verschiedenen chimären Proteinen hat nun erstmals gezeigt, dass auch der Faltungszustand von Polypeptiden Einfluss auf deren Translokation ins ER-Lumen haben kann. Die vorliegende Studie ergab, dass - unabhängig von der Primärstruktur - ein gewisses Maß an α-helikalen Bereichen notwendig für einen produktiven ER-Import ist. Sowohl die Länge der Polypeptide als auch posttranslationelle Modifikationen wie die GPI-Verankerung, die N-Glykosylierung oder die Ausbildung einer Disulfidbrücke beeinflussen die Translokation nicht. Darüber hinaus deuten die Ergebnisse der vorliegenden Arbeit darauf hin, dass Proteine mit ausgedehnten unstrukturierten Domänen am N-Terminus einer kotranslokationalen Qualitätskontrolle unterliegen und noch vor der Translokation ins ER-Lumen einer proteasomalen Degradierung im Zytosol zugeführt werden. Die in dieser Doktorarbeit dargestellten Ergebnisse legen daher die Vermutung nahe, dass die Ausbildung von Sekundärstrukturen vor oder während der Translokation die weitere Biogenese des naszierenden Polypeptids beeinflusst. Die gewonnenen Erkenntnisse können dazu beitragen die physiologischen aber auch die möglichen pathophysiologischen Konsequenzen der Regulation der Translokation besser zu verstehen. Der zweite Teil der Arbeit erbrachte erstmals experimentelle Evidenzen, dass trotz sehr geringer Sequenzhomologie zwischen den PrP-Homologen im Zebrabärbling (Danio rerio) und Säugetier-PrP die charakteristischen posttranslationalen Modifikationen, wie beispielsweise die komplexe Glykosylierung und der C-terminale GPI-Anker, konserviert sind. Die neu etablierten Zellkulturmodelle zur Analyse von PrP-homologen Proteinen deuten auf eine evolutionär konservierte Funktion von PrP hin und könnten dazu beitragen, neue Einsichten in die physiologische Aktivität von PrP zu gewinnen.

Ziel der vorliegenden Dissertationsarbeit war es, die mögliche Empfänglichkeit von Karpfen (Cyprinus carpio), Koi-Karpfen (Cyprinus carpio koi) und Zebrabärblingen (Danio rerio) gegenüber der Drehkrankheit mittels Nested-PCR und histologischen Untersuchungen zu klären. Zum Vergleich wurden Drehkrankheit-empfindliche Regenbogenforellen (Oncorhynchus mykiss) mit untersucht. Für die Untersuchungen zum Eintritt von Triactinomyxon-Sporen wurden Regenbogenforellen, Karpfen, Koi-Karpfen und Zebrabärblinge exponiert und jeweils 5 Minuten, 4, 6, 8, 12 und 18 Stunden, 1, 2, 3 und 5 Tage nach der Exposition die Parasitenstadien in der Schwanzflosse und in der Haut (an der lateralen Seite) der Forellen gezählt. Bei den Regenbogenforellen wurden in den Schwanzflossen nach fünf Minuten und vier Stunden Parasitenzahlen zwischen 16 bis 25 auf einer Fläche von 1 cm² pro Hautstück (an der lateralen Seite) gefunden, während bei allen drei untersuchten Cypriniden durchschnittlich 5 intakte eingedrungene Amöboidkeimzellen pro Fisch histologisch nachweisbar waren. In der Schwanzflosse und in der Haut von Karpfen, Zebrabärblingen, Koi-Karpfen und Regenbogenforellen war zu den Untersuchungszeitpunkten 1, 4 und 8 Stunden p.e. DNA von M. cerebralis nachweisbar. Nach acht Stunden waren bei den Regenbogenforellen und bei allen drei untersuchten Cypriniden die Ambödkeimzellen noch intakt. Außerdem war eine Abnahme der Anzahl an Parasitenstadien in der Haut nur bei den Regenbogenforellen zu detektieren. Ab 8 Stunden bis 60 Tage post expositionem konnte bei Regenbogenforellen in den Schwanzflossen ein Rückgang der Infektionsintensität festgestellt werden. In der Epidermis der Schwanzflossen war die durchschnittliche Triactinomyxon-Sporen-Dichte mit Abstand am höchsten. Es konnte jedoch zum Untersuchungszeitpunkt 60 Tage bei Regenbogenforellen Parasiten-DNA mittels Nested-PCR nachgewiesen werden, obwohl diese Fische bei der histologischen Untersuchung negativ getestet wurden. Ab 50 Tage post expositionem waren nur bei den Regenbogenforellen im Kopfknorpel Entwicklungsstadien von M. cerebralis und Veränderungen in Form von Degenerationen zu finden. Alle drei untersuchten Cypriniden wiesen im Kopfknorpel keine Entwicklungsstadien von M. cerebralis oder Schädigungen auf. Nach den Ergebnissen der vorliegenden Arbeit kann somit vermutet werden, dass viele Sporoplasmen in die Epidermis der Cypriniden eindrangen, aber den Knorpel nicht erreichen konnten. Der Beweis für die erfolgte Infektion des Schädelknorpels der Regenbogenforellen mit M. cerebralis wurde anhand der klinischen und histologischen Befunde durchgeführt, während in allen drei untersuchten Cyprinidenarten, der Parasit die Haut eindrangenx , aber nicht weiter entwickeln konnte. Die aus Hautproben, Schwanzflossen und Knorpelproben der infizierten Karpfen, Koi-Karpfen, Zebrabärblinge und Regenbogenforellen gewonnene DNA wurde mittels Nested-PCR amplifiziert. Dabei stellte sich heraus, dass die Nested-PCR deutlich sensitiver war als die histologische Untersuchung mit Hämatoxylin- und Eosin- Färbung. Aufgrund der vorliegenden Ergebnisse können weiterführende Untersuchungen durchgeführt werden, um die Resistenzmechanismen der Cypriniden gegenüber der Drehkrankheit klären zu können.

Fri, 19 Dec 2003 12:00:00 +0100 https://edoc.ub.uni-muenchen.de/5849/ https://edoc.ub.uni-muenchen.de/5849/1/Lang_Michael_R.pdf Lang, Michael ddc:500, dd