Podcasts about Epidermis

En esta edición hablamos de las historias y de los anclajes de los síntomas de Dermis y Epidermis desde la mirada de la Bioexistencia Consciente.

Another unscripted episode where I play only epic tracks. In this episode, hear epics from Anubis (AU), Cross (SE), Epidermis, Frumpy, Malibran, Nospun, Odd Logic, Pinnacle (US-PA), and U.K. Do you enjoy Prog-Scure? If so, perhaps you might consider helping me to keep this show afloat by contributing a few dollars at https://patreon.com/zapniles. Any donations […]

Kristin Eifler erzählt, wie sie gemeinsam mit ihrem Bruder Vincent einen Weg gefunden hat, ihre Neurodermitis vollständig zu heilen. Durch die Fokussierung auf das Nervensystem und dessen Beruhigung konnten sie ihre Hautgesundheit nachhaltig verbessern. In diesem Interview teilt Kristin ihre Erfahrungen und gibt wertvolle Tipps für Betroffene. Daily gut ist der fruchtige Drink von Braineffect, der deinen Darm und dein Immunsystem unterstützt. Mit dem Gutscheincode “bio360” bekommst du einen satten Rabatt! >>>Jetzt anschauen

When we talk about the multimodal approach to allergy management, a large portion of that is repairing the skin barrier. As the skin is the largest organ in the body and external, it is important to keep it healthy and hydrated. Pets with atopy have genetic defects in their skin that allow allergens to penetrate easily.But, how can you restore the skin barrier? Learn about three different ways on this week's episode of The Derm Vet podcast! 1. Topicals2. Nutrition3. SupplementsTIMESTAMPS00:00 Intro03:13 Topicals08:41 Topicals That Are Good For Restoring The Skin Barrier11:53 Nutrition14:13 Supplements16:33 Summary

Jeden Tag eine frische Unterhose – das gehört zur Werkseinstellung eines Erwachsenen. DACHTEN wir. Und dann kam diese Umfrage, Ende 2023: Jeder vierte Mann lässt den Wechselturnus hin und wieder schleifen und zieht nur jeden zweiten Tag ein frisches Höschen an. Oder jeden dritten. Ist das Thema Nachhaltigkeit an dieser Stelle wirklich gut platziert? Oder gibt es nicht doch gute Gründe, mindestens alle 24 Stunden nach frischer Wäsche zu kramen? Hygienestandards sind offensichtlich ganz unterschiedlich verteilt. Die einen rubbeln sich täglich zwei Mal unter der Dusche die obere Epidermis klinisch rein, die anderen lassen halt auch mal schleifen. Oder machen sowas wie „No Bathing“ – kein Deo, kein Duschgel, und nur einmal die Woche unter die Dusche springen. Wie viel Hygiene ist zu viel, wann muss man mal nachwischen, und machen wir uns vielleicht völlig umsonst verrückt? Das haben wir Dr. Iris Chaberny gefragt, Leiterin des Instituts für Krankenhaus- und Umwelthygiene am Universitätsklinikum Schleswig-Holstein. Eigentlich gibt es nur einen Grund für einen regelmäßigen Unterwäschewechsel, sagt Frau Dr. Chaberny, und der ist von sozialer Natur. Wer unangenehm riecht, hats im Kollegen- und Freundeskreis einfach schwerer. Krank wird man von Schweißrändern und Müffelbuxen eher nicht. Für Dominik Bloh war eine warme Dusche lange purer Luxus. 11 Jahre war der Hamburger obdachlos. Heute schreibt er Bücher und hat „Go Banyo“ gegründet. Ein umgebauter Linienbus mit 3 kleinen Bädern, in denen Wohnungslose eine viertel Stunde hinter sich abschließen und heiß duschen können. Denn wer sich nicht waschen kann, erzählt Dominik, der vereinsamt. Wer nicht gut riecht, bekommt keine Wohnung, macht keine Behördengänge, der isoliert sich und verliert seine Würde. Und hier Annes Studien: https://www.galaxus.de/de/page/deutschland-im-goblin-mode-jeder-vierte-mann-wechselt-seine-unterwaesche-nicht-taeglich-26725 https://www.galaxus.ch/de/page/koerperhygiene-umfrage-so-ungepflegt-ist-europa-30930 Und das hier legen wir euch ebenfalls ans Herz: OKF https://www.ardaudiothek.de/sendung/okf-ortskontrollfahrt/13548087/ und OZ. Graffiti-Künstler. Schmierfink. Rebell. https://www.ardaudiothek.de/sendung/oz-graffiti-kuenstler-schmierfink-rebell/14107933/ flexikon@ndr.de

In this thought-provoking episode, Christopher delves into the emotional turmoil of discovering that a once-admired figure in ministry has fallen from grace due to poor character and behavior. He reflects on the implications of such revelations, questioning the authenticity of faith and the importance of personal accountability before God. Join Christopher as he navigates feelings of disappointment, the dangers of idolizing others, and the necessity of maintaining a genuine relationship with God amidst the chaos of modern spirituality.

Send us a textIn this episode of Haus of PMU, I am beyond honored to sit down with the legendary Teryn Darling—a true pioneer in the permanent makeup industry. With over 24 years of experience, Teryn has transformed the PMU world through her innovation, education, and game-changing contributions.We cover it all:The challenges and rewards of studio transitions.How to recognize and overcome burnout as a PMU artist.The story behind her brand-new OMG® Brow Pigment Line with Li Pigments.Why her Beyond the Epidermis Color Theory Course is a must-have for every artist (plus an exclusive discount code below!).Teryn has generously provided our listeners with 50% off her Beyond the Epidermis course—a comprehensive guide to mastering color theory that applies to any pigment line. Use the code BROWHAUS50 at checkout to claim your discount!Tune in to hear Teryn's invaluable insights, her passion for elevating the industry, and her advice for artists at every stage of their journey.Resources Mentioned in this Episode:Beyond the Epidermis Color Theory Course: https://www.girlzinktraining.com/beyond-th-epidermis-courseOMG® Brow Pigment Line: https://store.girlzink.com/products/omg-brow-pigment-kit-pre-order-nowTatter-a-fact® Podcast: https://podcasts.apple.com/us/podcast/tatter-a-fact-pmu-podcast/id1563510666Girlz Ink Online Store: www.girlzink.comGirlz Ink Online Academy: https://www.girlzinktraining.comFollow Teryn Darling on Instagram:@girlzinkstudio@tatgirlzink@tatterafactpodcastFollow Christa Nordquist (Haus of PMU) on Instagram:@browhaustulsa@browhausacademyVisit the Haus of PMU Website & Store:www.browhaustulsa.comHaus of PMU Shopify StorefrontFor any inquiries, episode questions, or guest requests, reach out at:Email: info@browhaustulsa.comUpcoming PMU Fundamentals Training:My next PMU Fundamentals training starts in February 2025, and we're offering a HUGE discount for anyone who signs up by January 15, 2025. Don't miss out on this amazing opportunity to get the training you need to take your PMU career to the next level!If you enjoyed this episode, don't forget to follow the show, share it with your artist friends, and leave a review if you found this episode helpful and meaningful. I might even share your review on the show!Thank you, Teryn, for sharing your knowledge and inspiring us all. This episode is packed with wisdom, so don't miss it!

En este episodio, profundizamos en la epidermis, la capa más externa de la piel y nuestra primera línea de defensa contra agentes externos. Exploramos sus cinco subcapas, sus funciones esenciales y cómo los tratamientos y productos específicos pueden optimizar su salud. Descubre cómo exfoliar, hidratar y proteger adecuadamente esta capa para obtener resultados visibles en tus clientes.

¿Cómo es la piel de nuestras mascotas y qué funciones tiene? La piel del perro y el gato es una membrana flexible que cubre todo su cuerpo, representando entre el 12 y el 24% de su peso total, lo que la convierte en su órgano más extenso. Funciona como una barrera protectora contra diversos patógenos. Este escudo natural tiene un grosor que varía entre uno y cinco milímetros, según la zona del cuerpo, y apenas contiene glándulas sudoríparas. Es el principal receptor de estímulos como frío, calor, contacto, dolor o picazón.Sin embargo, la piel de los perros y los gatos guardan muchas similitudes. Ambas se componen de tres capas: Epidermis, que es la capa externa y está en contacto directo con el exterior. Dermis, situada bajo la epidermis, más gruesa y que contiene vasos sanguíneos, linfáticos, nervios, glándulas sebáceas y sudoríparas, además de folículos pilosos. Hipodermis, la capa más profunda. Compuesta principalmente de tejido graso.Eso sí, si hay un aspecto que ...

Psoriasis explained, including the different types and treatment options for each. Also includes psoriasis pathophysiology, causes and aggravating factors. Consider subscribing on YouTube (if you found any of the info useful!): https://www.youtube.com/channel/UCRks8wB6vgz0E7buP0L_5RQ?sub_confirmation=1Patreon: https://www.patreon.com/rhesusmedicineBuy Us A Coffee!: https://www.buymeacoffee.com/rhesusmedicineTimestamps:0:00 What is Psoriasis?0:21 Psoriasis Pathophysiology1:57 Psoriasis Causes and Triggering Factors3:42 Psoriasis Symptoms & Psoriasis Types7:28 Psoriasis Diagnosis8:10 Psoriasis TreatmentReferencesJones, C (2023) - DermNetNZ - “Psoriasis”. Available at https://dermnetnz.org/topics/psoriasisWu, B (2017) - DermNetNZ - “Drug-Induced Psoriasis”. Available at https://dermnetnz.org/topics/drug-induced-psoriasisNational Psoriasis Foundation (2022) - “Psoriasis: Causes & Triggers”. Available at https://www.psoriasis.org/causes/British Association of Dermatologists (2018) - “PSORIASIS – AN OVERVIEW”. Available at https://cdn.bad.org.uk/uploads/2021/12/29200253/Psoriasis-an-overview-update-October-2018-Lay-review-October-2018.pdfWitold Owczarek (2022) - “The role of HLA-Cw6 in psoriasis and psoriatic arthritis**”.** Available at https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9661405/Psoriasis Association (2016) - “Types of Psoriasis “. Available at https://www.psoriasis-association.org.uk/media/InformationSheets/Types.pdfAmerican Academy of Dermatology Association - “Management of Adult Psoriasis with Topicals”. Available at https://assets.ctfassets.net/1ny4yoiyrqia/56bNCO2qMLxBtICdr9biM6/204ac5f5d98243e116bce0b5725b9f92/AAD-Guideline-Topical-Psoriasis-Clinical-Pathway-May-2023-Update.pdfHani, Y - (2024) - “Anatomy, Skin (Integument), Epidermis”. Available at https://www.ncbi.nlm.nih.gov/books/NBK470464/#:~:text=The epidermis, the skin's outermost layer, is composed ofRendon, A (2019) - “Psoriasis Pathogenesis and Treatment**”.** Available at https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6471628/#:~:text=Our discussion begins by addressing the inflammatoryPlease remember this podcast and all content from Rhesus Medicine is meant for educational purposes only and should not be used as a guide to diagnose or to treat. Please consult a healthcare professional for medical advice. 

Kinds of Kindness | Verliebt in die Epidermis von Jesse Plemons Gut ein halbes Jahr nach dem hiesigen Kinostart des mehrfach Oscar-prämierten Films "Poor Things" bringt der griechische Arthouse-Regisseur Yorgos Lanthimos am 4. Juli 2024 bereits seinen nächsten Film "Kinds of Kindness" in unsere Kinos. Der fast drei Stunden lange Episodenfilm ist vollgepackt mit Stars und skurrilen Elementen. Die Handlungen drehen sich um einen Angestellten, dem von seinem Chef alles vorgeschrieben wird, eine Sekte mit bizarren Sauna-Ritualen und einen Ehemann, der seine Frau im wahrsten Sinne des Wortes zum Fressen gern hat. Ob der Film mit Jesse Plemons, Emma Stone und Willem Dafoe mit Lanthimos' besten Werken mithalten kann, darüber diskutieren in dieser Besprechung Lida und Stu. Es geht los, sobald ihr auf Play drückt. Viel Spaß mit der neuen Folge vom Tele-Stammtisch! Trailer Werdet Teil unserer Community und besucht unseren Discord-Server. Dort oder auch auf Instagram könnt ihr mit uns über Filme, Serien und vieles mehr sprechen. Wir liefern euch launige und knackige Filmkritiken, Analysen und Talks über Kino- und Streamingfilme und -serien - immer aktuell, informativ und mit der nötigen Prise Humor. Website | Youtube | PayPal | BuyMeACoffee Großer Dank und Gruß für das Einsprechen unseres Intros geht raus an Engelbert von Nordhausen - besser bekannt als die deutsche Synchronstimme Samuel L. Jackson! Thank you very much to BASTIAN HAMMER for the orchestral part of the intro! I used the following sounds of freesound.org: 16mm Film Reel by bone666138 wilhelm_scream.wav by Syna-Max backspin.wav by il112 Crowd in a bar (LCR).wav by Leandros.Ntounis Short Crowd Cheer 2.flac by qubodup License (Copyright): Attribution 4.0 International (CC BY 4.0) Folge direkt herunterladen Folgt uns ab sofort regelmäßig live auf Twitch: twitch.tv/dertelestammtisch

Agradece a este podcast tantas horas de entretenimiento y disfruta de episodios exclusivos como éste. ¡Apóyale en iVoox! Subterranea Rarities con Carlos Romeo, Carles Pinós y David Pintos. Episodio dedicado a National Health; al fabuloso disco de Salem Hill, The Robbery of Murder; y a las bandas setenteras Epidermis, Guns and Butter y Byzantium. Disfruta de las excelentes rarezas seleccionadas por los «rarities» de Subterranea. Edición: David Pintos www.subterranea.eu Libros de Subterranea Libros Magazine para América y España en: www.davidpintos.com Escucha este episodio completo y accede a todo el contenido exclusivo de Subterranea Podcast. Descubre antes que nadie los nuevos episodios, y participa en la comunidad exclusiva de oyentes en https://go.ivoox.com/sq/17710

Thanks to Cecilio for sending in this question. James Tytko asked University of Leeds Professor of Dermatology, Julia Newton Bishop, for assistance... Like this podcast? Please help us by supporting the Naked Scientists

Beim Sonnenbrand hat sich die Unterhaut entzündet, die betroffenen Körperpartien sind rot und warm, sie schmerzen – und doch ist das alles nur ein Alarmsignal für eine weitaus größere Gefahr.

Imagine a suit that fits you perfectly, doesn't wrinkle for years, repairs itself, is waterproof, has air conditioning and heating, and protects you against damage and radiation. And it's all for free. You're wearing it now. This is your skin. The human body is a beautiful and mysterious machine. We collect the most interesting facts about the human body, and today, we're going to figure out how the human skin works. So, your skin consists of three layers: the Epidermis, Dermis, and Hypodermis. The epidermis is the uppermost layer of your skin. This is your personal waterproof armor that protects the body from bacteria and damage. The lifespan of this armor isn't long, but the old cells are completely replaced with new ones after 4-6 weeks. By the way, half of the dust found in your house is old skin cells! Yuck!... But let's go deeper and find out what your skin is made of, why it's so sensitive, why the skin on your scars looks a little different, and some other interesting facts about the human body's largest organ. Learn more about your ad choices. Visit megaphone.fm/adchoices

“So, it's a very expensive process. It has a lot of inefficiencies, and a lot of those costs are due to failures. I think we are at the point where so many of these methods have shown in studies to be more predictive for humans that it really is an issue of human health. We need to do better for patients we know we can and that these methods exist, so we need to use them.” This past season, we've focused quite a bit on animal research and experimentation, and a lot of our focus has been on just how horrible it is, how terrible it is for the animals. And a lot of our focus has been really on just how bad it is, how bad it is for the animals, how bad it is for science, and how bad it is for taxpayers who are spending so much money on this stuff. What we haven't focused enough on are solutions. And there are solutions. There are many many human relevant methods that are here to replace animal testing and there are many more on the way. Elizabeth is the director of research policy for the Physicians Committee for Responsible Medicine. They're a nationwide organization of physicians and laypersons that promote preventative medicine. They conduct clinical research, and they advocate for more effective, efficient and ethical medical research, product testing and training. pcrm.org

Savanna and Tyler give the skinny on skin in part one of this Q&A series.Shop now HERE.

Savanna and Tyler give the skinny on skin in part one of this Q&A series.Shop now HERE.

Anna and Shannon get deeper than the Epidermis into how much is too much, before it's too far. After a break from Annas incident were back to talk about whats HOT in the MedSpa industry, all the talk is moving towards a more NATURAL look. Who's going to tell you when enough is enough? You need to trust you're in good hands! Hear it here first from Shannannagans with Camas MedSpa

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2023.03.07.531620v1?rss=1 Authors: Zaver, S. A. A., Sarkar, M. K., Egolf, S., Zou, J., Tiwaa, A., Capell, B. C., Gudjonsson, J. E., Simpson, C. L. Abstract: Mutation of the ATP2A2 gene encoding sarco-endoplasmic reticulum calcium ATPase 2 (SERCA2) was linked to Darier disease more than two decades ago; however, there remain no targeted therapies for this disorder causing recurrent skin blistering and infections. Since Atp2a2 knockout mice do not phenocopy its pathology, we established a human tissue model of Darier disease to elucidate its pathogenesis and identify potential therapies. Leveraging CRISPR/Cas9, we generated human keratinocytes lacking SERCA2, which replicated features of Darier disease, including weakened intercellular adhesion and defective differentiation in organotypic epidermis. To identify pathogenic drivers downstream of SERCA2 depletion, we performed RNA sequencing and proteomic analysis. SERCA2-deficient keratinocytes lacked desmosomal and cytoskeletal proteins required for epidermal integrity and exhibited excess MAP kinase signaling, which modulates keratinocyte adhesion and differentiation. Immunostaining patient biopsies substantiated these findings with lesions showing keratin deficiency, cadherin mis-localization, and ERK hyper-phosphorylation. Dampening ERK activity with MEK inhibitors rescued adhesive protein expression and restored keratinocyte sheet integrity despite SERCA2 depletion or chemical inhibition. In sum, coupling multi-omic analysis with human organotypic epidermis as a pre-clinical model, we found that SERCA2 haploinsufficiency disrupts critical adhesive components in keratinocytes via ERK signaling and identified MEK inhibition as a treatment strategy for Darier disease. Copy rights belong to original authors. Visit the link for more info Podcast created by Paper Player, LLC

Another special & extended episode where I break from routine and feature music from artists both Prog-Scure and famous. In each episode of this series, I'm playing one half (one full side) of outstanding and influential albums throughout rock ‘n' roll history, regardless of sub-genre. In this episode, hear diverse artists including 10cc, Epidermis, Freedom, […]

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2022.09.07.507048v1?rss=1 Authors: Coakley, S., Bonacossa-Pereira, I., Hilliard, M. A. Abstract: Spectrins are highly conserved molecules that form a distinct membrane associated periodic scaffold within axons thought to provide mechanical resilience. In C. elegans, UNC-70/{beta}-Spectrin also functions outside the nervous system, within the epidermis, to maintain the integrity of sensory neurons. The precise molecular organization and cellular mechanisms that mediate this protection are unknown. Here, using 3D-structured illumination microscopy, we show that within the epidermis spectrins form a crescent-shaped scaffold with a periodicity of ~ 200 nm that embraces adjacent axons. This epidermal spectrin scaffold is induced by developing axons and reformed during axonal regeneration, and creates a molecular imprint of the adjacent nervous system. Disruption of this epidermal scaffold causes widespread axonal damage in sensory and motor neurons. These findings reveal the existence of a distinct and periodic spectrin framework within the epidermis that is molded by the developing nervous system and is necessary to protect it from mechanical damage. Copy rights belong to original authors. Visit the link for more info Podcast created by PaperPlayer

Our skin is an incredibly important, multifunctional organ. It has many different functions including helping us maintain our body temperature, it assists with the excretion of water and toxins, and most importantly, protective mechanism of acting as a barrier. But in newborns, especially preterm infants, there are developmental differences in the anatomy and physiology of their skin that place them at an increased risk for skin injury. Not only is the immaturity of an infant's skin problematic, but infants in the NICU are predisposed to skin trauma due to the life-saving monitors and equipment necessary for their care. Furthermore, infants with compromised skin integrity are at an increased risk for infection, excess water loss, absorption of toxic substances, and additional sequelae. On this episode, we review the basic anatomy of our skin and all of its important functions. We discuss how the skin of a term and preterm infants differs from adults and why the structural variations place infants at an increased risk for skin trauma and additional complications. The episode will be beneficial for parents of infants, especially preterm infants or those in the NICU to understand why their infant is at an increased risk for compromised skin integrity. NICU clinicians will also hear a great reminder of the anatomical and physiologic variations in infants that place them at an increased risk for skin injury. Our NICU Roadmap: A Comprehensive NICU Journal: https://empoweringnicuparents.com/nicujournal/NeoTech Free Samples: neotechneoshades.comNICU Mama Hats: https://empoweringnicuparents.com/hats/NICU Milestone Cards: https://empoweringnicuparents.com/nicuproducts/Empowering NICU Parents Show Notes: https://empoweringnicuparents.com/episode34Empowering NICU Parents Instagram: https://www.instagram.com/empoweringnicuparents/Empowering NICU Parents FB Group: https://www.facebook.com/groups/empoweringnicuparentsPinterest Page: https://pin.it/36MJjmH

You hear dermatologists mention the "multimodal approach to allergies" but what does that mean beyond prescribing an anti-itch therapy? Find out why the epidermal barrier matters and how you can help restore it for your itchy patients.

Dr. Elizabeth Dinsdale, Matthew Flinders Fellow in Marine Biology in the College of Science and Engineering at Flinders University in Adelaide, Australia, uses genomic techniques to investigate the biodiversity of microbial communities in distinct ecological niches, including coral reefs, kelp forest and shark epidermis. She discusses how shotgun metagenomics is being used to characterize the architecture of microbial communities living in the thin layer of underlying mucus on shark's skin, and how understanding the function of these microbes is providing clues to important host-microbe interactions, including heavy metal tolerance. Ashley's Biggest Takeaways: Sharks belong to a subclass of cartilaginous fish called elasmobranchs and are unique in that their epidermises are covered in dermal denticles—overlapping tooth-like structures that reduce drag and turbulence, helping the shark to move quickly and quietly through the water. These dermal denticles are sharp (if you're going to pet a shark, make sure you go from the head to the tail to avoid getting cut!), and depending on the species of shark, may be more or less spread out across the epidermis. Where do microbes enter the story? Dermal denticles overlay a thin layer of mucus, which provides a distinctive environment for microbial life. Collecting microbial samples from underneath a shark's dermal denticles is quite difficult, and the technique varies by shark species (shark size, water depth and ability to bite all factor into the equation). Liz's team uses a specially designed tool that the group affectionately calls a “supersucker,” to create and capture a slurry of microbes and water for analysis. The team then uses shotgun metagenomics to identify and characterize the microbes in their collected samples. Sequencing has revealed biogeographical difference, as well as similarities in microbial architecture of whale sharks across the globe. There are 2 populations of whale sharks—one in the Atlantic Ocean and the other in the Indian Pacific Ocean. Samples collected from both populations have revealed that each individual whale shark, from within each aggregation, shares many of the same microbes. In fact, unlike algae which may share 1 to 2 microbial species, whale sharks share about 80% of microbes across every individual. Since many of the sharks don't cross aggregations, Liz's team is investigating the possibility of coevolution between microbes and hosts. Metagenomic sequencing also provides information about the function of the sequenced microbes. High presence of heavy metal-tolerant microbes has been found in the epidermis of all shark species that the team has analyzed. Sharks are known to carry high levels of heavy metals in their skin, muscle and even blood. However, muscle tissue samples contain lower concentrations than skin, indicating that there may be a density gradient in place, and raising questions about how microbes might be involved in this regulation. Is there a pathway by which the microbes metabolize and help to remove concentrations of heavy metals across the epidermis? Liz and her team are hoping to find out. Links: Elizabeth Dinsdale https://www.flinders.edu.au/people/elizabeth.dinsdale Tracking Pathogens via Next Generation Sequencing (NGS) https://asm.org/Magazine/2021/Spring/Tracking-Pathogens-via-Next-Generation-Sequencing Microbial Ecology of Four Coral Atolls in the Northern Line Islands https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0001584 Coral Research https://coralandphage.org/research_coral.php  Metagenomic analysis of stressed coral holobionts https://pubmed.ncbi.nlm.nih.gov/19397678/  Metagenomic analysis of the microbial community associated with the coral Porites astreoides  https://pubmed.ncbi.nlm.nih.gov/17922755/

Michele De Luca, MD, shares his research in gene therapy for epidermolysis bullosa and cell therapy for ocular burns. Series: "Stem Cell Channel" [Health and Medicine] [Science] [Show ID: 37671]

Michele De Luca, MD, shares his research in gene therapy for epidermolysis bullosa and cell therapy for ocular burns. Series: "Stem Cell Channel" [Health and Medicine] [Science] [Show ID: 37671]

Michele De Luca, MD, shares his research in gene therapy for epidermolysis bullosa and cell therapy for ocular burns. Series: "Stem Cell Channel" [Health and Medicine] [Science] [Show ID: 37671]

Michele De Luca, MD, shares his research in gene therapy for epidermolysis bullosa and cell therapy for ocular burns. Series: "Stem Cell Channel" [Health and Medicine] [Science] [Show ID: 37671]

Michele De Luca, MD, shares his research in gene therapy for epidermolysis bullosa and cell therapy for ocular burns. Series: "Stem Cell Channel" [Health and Medicine] [Science] [Show ID: 37671]

Michele De Luca, MD, shares his research in gene therapy for epidermolysis bullosa and cell therapy for ocular burns. Series: "Stem Cell Channel" [Health and Medicine] [Science] [Show ID: 37671]

Michele De Luca, MD, shares his research in gene therapy for epidermolysis bullosa and cell therapy for ocular burns. Series: "Stem Cell Channel" [Health and Medicine] [Science] [Show ID: 37671]

Michele De Luca, MD, shares his research in gene therapy for epidermolysis bullosa and cell therapy for ocular burns. Series: "Stem Cell Channel" [Health and Medicine] [Science] [Show ID: 37671]

Michele De Luca, MD, shares his research in gene therapy for epidermolysis bullosa and cell therapy for ocular burns. Series: "Stem Cell Channel" [Health and Medicine] [Science] [Show ID: 37671]

“OK, I mean this is an item that you're laying your entire EPIDERMIS on every single night. Wash your sheets by themselves” -JasonJoin Brigid and Elizabeth as they learn all of the things they've been doing “insufficiently” when it comes to laundry, and more importantly, how to tackle the task correctly.  Jason Ramos, guru of all things house and home, joins the gals for a very illuminating chat discussing everything from mastering the basic steps of laundry (pre-wash, wash, dry) to why you might not be getting the detergent completely rinsed or it takes for-ev-er for your load to dry! And also, pop over to our Instagram to see Jason fold the perfect fitted sheet. Jason's tips: Pre-Wash: Sort by color, then by use/size. Like colors + fabrics wash together. Whites = higher temp. Darks=cold. Large/bulky = wash alone. Wash: Temps are cold, warm, hot. Modes are delicate, permanent press, regular. Hot = anything you want to sanitize (sheets, towels, etc.) Cold = color-rich, dark clothes made of cotton or woven natural fabric. Delicate = bras, silks, fragile items. Regular = everything else! Dry: Different clothes require different drying! Drying rack = delicate sweaters, bras, loosely woven fabrics. Dryer = most things. Sunlight & fresh air = Jason's pick for drying anything!

There is controversy in Green Bay and something happened in Cleveland. Learn more about your ad-choices at https://www.iheartpodcastnetwork.com

Most tattoos tell a sentimental story. This episode tells the story of what happens in our body when we get a tattoo. Alex Machado, a tattoo artist who has been in the industry and tattooing for 28 years, describes the anatomy of a tattoo. He explains where the ink goes in the skin, how the body accepts the ink, and how and why a tattoo really is permanent. Alex also details how different it is to tattoo various skins, such as wrinkly or thick skin. While the audio quality isn't what you are used to hearing from The Anatomy Nerd Podcast, I encourage you to be patient and tune in to this fascinating and fun episode. Alex has a lot to share, and we tried our very best to get clear audio. Buzzsprout - Let's get your podcast launched! Start for FREEDisclaimer: This post contains affiliate links. If you make a purchase, I may receive a commission at no extra cost to you.Support the show (https://www.patreon.com/theanatomynerdpodcast)

We talked about our Gut, we talked about our homes, we are about to get granular. Epidermis. Who remembers that word from High school Biology? Why is it so important to be aware of what we put on our skin? Our friendly science teacher Raquel reminds us that it is our first line of defense. Its not just about that tan girl! Listen in as we talk about our largest organ, Our Skin! Having issue's deciding what's safe to buy while reading those long labels. Download the app Think Dirty wherever you get your apps. Raquel and I want to inspire, inform and encourage you to take that small step, make that 2 degree pivot towards loving your planet not just living on it. It's a Mindset! Flip that Script. Reduce, Reuse and then Recycle. Consider it this way, It's Not just an inconvenience, it's an investment in the future generations. Take the quiz to find out if your home is toxic at www.shellyswellness.com Find us on Instagram @earthygirl.co --- This episode is sponsored by · Anchor: The easiest way to make a podcast. https://anchor.fm/app --- Send in a voice message: https://anchor.fm/rebecca-behnke/message

Murph is joined by Prue Blake and Nick Quon to chat about the Season 6 episode Bart of Darkness

Es ist ein heißer Sommer in Springfield! Maulwürfe brennen, die Chiliverkäufe schießen in den Keller und alle sehnen sich nach Erfrischung. Was könnte da besser Abhilfe schaffen als ein eigener (Mil)Pool! Leider hat Bart nichts von dem Spaß, weil er sich das Bein gebrochen hat. Deswegen sitzt er allein und verbittert in seinem Zimmer, bis er etwas Schockierendes im Hause der Flanders mit ansieht... Was es ist, erfahrt ihr in dieser Folge. Viel Spaß und passt auf eure Epidermis auf! Euer Marcpool und die Königin des Sommers.

Herzlich Willkommen, da ist unsere Haut das größte Organ des menschlichen Körpers und dennoch pflegen wir es nur an ausgewählten Stellen hingebungsvoll und sehr sorgfältig. Was ist denn, wenn die anderen Hautpartien trocken und rissig sind, jucken und Probleme bereiten. Dann liegt es häufig daran, dass der Wassergehalt der Haut von 20% auf unter 10% abgesackt ist - deine Haut schlägt Alarm. In diesem Video erzählen wir dir, aus welchen Schichten deine Haut besteht, warum du eigentlich nur die Epidermis - griechisches Wort für „auf der Haut“ - pflegen musst und warum Jojoba- und Mandelöl nach dem Duschen absolut nicht zu empfehlen sind. Sophie Friedrich ist seit 2013 als PTA bei der MediosApotheke beschäftigt und lebt dort ihre Leidenschaft für Kosmetik voll aus. Wer kommt da auf seine Kosten? Unsere Kunden natürlich. Sophie ist das geborene Beratungstalent, kennt jede Hautschicht aus dem ff und ist Expertin für Kosmetik und Dermatologie. Sophies Pflegetipps gehen quasi unter die Haut und Bakterien, Viren und Pilze haben keine Chance mehr. Aus rechtlichen Gründen weisen wir darauf hin, dass dieses Video keinen Arztbesuch ersetzt oder eine medizinische Behandlung darstellt. Alle Aussagen in diesem Video wurden sorgfältig recherchiert und nach bestem Wissen und Gewissen wiedergegeben und geben den aktuellen Stand der Wissenschaft wieder. Wissenschaftliche Erkenntnisse können sich jedoch im Laufe der Zeit ändern und dadurch können Aussagen in diesem Video im Laufe der Zeit teilweise nicht mehr dem aktuellsten Stand der Wissenschaft entsprechen. Dafür übernehmen wir keine Gewährleistung.

This episode covers epidermis layers!

https://www.instagram.com/drkasten/ Zunächst einmal möchten wir einen Irrtum aufklären☝

Nuestro capitulo de hoy lo dedicamos a hablar sobre: Rejuvenecimiento facial sin cirugía con Bioestimuladores y Hifu con el Cirujano Plástico, Rubén Pérez Parra (Dr.PerezParra). El tratamiento con bioestimuladoores es un combinado inyectable denominado Sculptra (ÁcidoPolilaptico), de @Sofimedical_Ve, que se aplica con el fin de estimular la regeneración de los tejidos y la síntesis de colágeno. Gracias a este tratamiento "anti-edad" se activan y revitalizan los procesos metabólicos de la dermis. El objetivo es restaurar los componentes estructurales de la dermis reduciendo la flacidez, potenciando la tersura y la elasticidad y reduciendo los signos del envejecimiento de la piel. Por su parte el Hifu, es un equipo de “Hight Intensify Focused Ultrasound” o Ultrasonido Focalizado de Alta Intensidad, que como su nombre mismo lo indica es un tratamiento basado en ultrasonido que focaliza la energía en un solo punto para actuar de forma precisa. Su fin es generar un calentamiento controlado en el tejido y actuar directamente en el nivel del “Sistema Músculo Aponerótico Superficial”, es decir, la membrana que recubre los músculos de la cara. Cuando se produce ese calentamiento interno, se da una contracción inmediata de las fibras de colágeno existentes, así como la neocolagénesis y elastogénesis a medio plazo a la vez que permite incidir en todos los niveles: Epidermis, dermis superficial y profunda. Se producen micro lesiones que aumentarán la capacidad de regeneración natural de los tejidos. Los resultados del tratamiento inmediato es una mejora visible de la piel pero cuando realmente se apreciaran es a partir de los 21 días que es el momento en el que se generará el nuevo colágeno, pasado 1 mes, la piel mejora el aspecto y textura, se aprecia más clara, el tejido se ve más compacto y se percibe un ligero lifting.

Everyone, the dads in Stoneybrook SUUUUUUUUUUUCK and—surprise!—Emily has some thoughts about it! We dive into queer theory and Kristy’s question of “what makes a family?” as we meet Emily Michelle and discuss Anne’s affinity for all things carnival-related.

¡Bienvenida a la serie Conociendo tu Tu Tipo de Piel! Este episodio da comienzo a la serie de Conociendo Tu Tipo de piel, pero antes de comenzar hablar de cada tipo de piel, debes saber un poco de cómo Tu piel funciona como está compuesta tu piel. Habló sobre la Epidermis y la Dermis. Espero que te pueda ayudar durante esta serie a que identifiques tu tipo de piel. Espero que les guste recuerda que puedes ver la notas en mi blog de www.cuidatupielhoy.com  

Confessions of a YES woman...

This episode on "The Process" I talk with composer and multi-instrumentalist Dan VanHassel. We listen to his work  "Epidermis" from Transient Canvases' "Wired" album release. Dan and I discuss sampling, working with noise, and the magic of live performance.

From Human Saliva to Covalent Bonds, Vectors to Atomic Numbers, improve your Science Trivia Skills from the comfort of your own home.Get your trivia training up to date as you prepare for greatness.Trivia Podcast.Immerse yourself in trivia training.Level up your Trivia skills the easy way with Pub Quiz Prep.Pub Quiz Prep - Putting the 'edge' in your general knowledge.Invest in success with Pub Quiz Prep.The education you want, the education you deserve.https://rss.whooshkaa.com/rss/podcast/id/5093https://www.pubquizprep.com/https://www.youtube.com/c/PubQuizPrephttps://www.instagram.com/pubquizprep/?hl=enhttps://twitter.com/PubQuizPrephttps://www.tumblr.com/blog/pub-quiz-prephttps://podcasts.google.com/?feed=aHR0cHM6Ly9yc3Mud2hvb3Noa2FhLmNvbS9yc3MvcG9kY2FzdC9pZC81MDkzhttps://open.spotify.com/show/1MzCLQMR64F3oztAeNBf4UWhat does MS stand for?

From Human Saliva to Covalent Bonds, Vectors to Atomic Numbers, improve your Science Trivia Skills from the comfort of your own home.Get your trivia training up to date as you prepare for greatness.Trivia Podcast.Immerse yourself in trivia training.Level up your Trivia skills the easy way with Pub Quiz Prep.Pub Quiz Prep - Putting the 'edge' in your general knowledge.Invest in success with Pub Quiz Prep.The education you want, the education you deserve.https://rss.whooshkaa.com/rss/podcast/id/5093https://www.pubquizprep.com/https://www.youtube.com/c/PubQuizPrephttps://www.instagram.com/pubquizprep/?hl=enhttps://twitter.com/PubQuizPrephttps://www.tumblr.com/blog/pub-quiz-prephttps://podcasts.google.com/?feed=aHR0cHM6Ly9yc3Mud2hvb3Noa2FhLmNvbS9yc3MvcG9kY2FzdC9pZC81MDkzhttps://open.spotify.com/show/1MzCLQMR64F3oztAeNBf4UWhat does MS stand for?

Welcome back to The Beauty Pitch for Episode 17 Quick Reminder: This podcast is intended for a MATURE AUDIENCE. As we don't cuss like crazy there might be some words that grace your presence that could be described as profanity. Also to be noted we try to be as factual as possible so we apologize in advance if there is something we are not completely correct on. I do try and correct them in the facts section in the details but still we are human and might miss somethings. Lastly some of the things we discuss are opinions. Other professionals might have a different take on things and we are okay with that. This is what we have found to work for us and our clients. So that being noted we shall move on. This Episode we roll out out new segment called flip the scrip where Yvonne answers skin questions and Kristen answers hair questions. What is the Epidermis...? What layer is the Epidermis and what is in that layer...? What is the layer of skin that is under the Epidermis...? What layer can a non medical aesthetician affect...? As a hair colorist and I am taking out hair color what comes up when I am bleaching the hair...? As natural underlying pigment is coming up what are the natural color that will come up first if they are a lever 3...? What do you tone red color with...? What is the most stubborn form of pigmentation...? Why is melasma so stubborn...? When coloring grey what pigments do you have to put back into the hair in order to get the best coverage...? If I have acne what is something I cold do to help it...? What happens to the skin about once a month...? When determine the right style or haircut for a client what is the best way to determine that...? What is the best tool when cutting someones hair...? What is a chemical peel...? Who would get this service done...? I would like to have a brazil lain blowout treatment how long does it last...? How does it work...? Yvonne unboxes her fab fit fun box and we give tips on how to use the products... --- This episode is sponsored by · Anchor: The easiest way to make a podcast. https://anchor.fm/app

Wie tief ist porentief? Was ist Puddinghaut? Und hattet ihr schon mal Epidermis?

In this episode, Laura and John from Herb Tech Pharmaceuticals join the podcast to discuss their new line of CBD topicals with Penetrating Topical CBD Phase-change Technology designed to be absorbed rapidly, provide relief and enhance your lifestyle. I enjoyed hearing about how their business got started and the passion this company has for bringing to market products that are first class. They have six different topicals, each infused with 1000 mg of CBD, and dose controlled so a pump delivers 20 mg. A bit of information how Herb Tech Pharmaceuticals came about: "Our development took a different pathway and having been focused on the pharmaceutical sector, specifically with drug delivery technology, and in fact, working with a specialty pharmaceutical development company in Boston mass that works on transdermal drug delivery technology, thought that the application of principles developed there with that technology could be directly applicable for CBD. One of the big problems obviously with CBD is that if you smoke it with all the problems with COVID-19 and everything else, obviously smoking is harmful to the lungs. If you are ingesting it, the onset of it is terrible and you've got to push the active components through the walls, into your stomach then you have first pass liver first effect and thereafter, in which case it's highly inefficient and can be problematic as well. Topicals and transdermals is could be an interesting pathway, it's safe, but you need technology to be able to get the CBD to the receptors, cannabidiol receptors. And when Laura approached me and said, Gee, is there a way we could use the technology or develop other technology that would allow us to create a company that could effectively create products to have the benefits of the CBD and other botanicals as well, but the advanced technology that allowed the CBD and the botanicals to interact much more efficiently with the human body, that's how our tech was born. We function on the same focus as really a pharmaceutical business, but just applied to CBD in botanicals."Laura goes on to explain further: "So currently, CBD, there's so many health benefits to it, we all know it. However, the side effects of current delivery methods don't overcome the benefits of the CBD. Smoking, the amount of CBD that's needed to ingest to have an effect, because again, as John mentioned, when your stomach sort of chews up most of the CBD in your stomach, you're actually getting very little into your system. And then there's a side effects of having such a high dose that you're having to take. So there's stomach upset. There's onset of action, it can have effect in 10 minutes, or it can be six hours, right? It's very unpredictable. So those two methods, they weren't efficient and they weren't working. So we said Wow, why can't we take all the wellness of botanicals, not just CBD, all herbal extracts, and really make them more effective, because we all want to use nature. But we have to be able to receive that nature, to receive it safely through the skin, which again, will not happen unless you have some sort of emulsion technology, it will just sit on the outer layer of the skin, that dead skin and it won't have an effect internally. So we're trying to create something that's only effective, but it's safe, and we've priced it so people can afford it. So I'm really excited to introduce this line of products to folks."Check out the video on our phase change technology: https://herbtechpharma.com/technology/Laura continues with a description of their product line: "There are a lot of products out there, one size fits all, take CBD for everything. Herbal extracts can help you in certain lifestyle situations throughout your day. So we created six different need states with other botanicals infused in this delivery system. One is "be sleepy" with melatonin, chamomile, lavender "be calm" with a St. John's wort. We have "be active" with white willow, which is a precursor to aspirin, for you know to put on muscles and joints. We have a "be intimate" which is very different. And that's again infused with Red Ginseng. We have "be healthy" and to "be empowered". And again all these are not just infused with botanicals but this is something that we're very proud of. 1000 Mg's of CBD hemp extract, and again just because someone says they have 1000 mg of extract You have no idea how much CBD is in that extract. You have no idea unless the manufacturer tells you and we tell you on our labels 80 to 95% pure, and then you go to our website, there's a QR code on our bottles. We're very proud of our labels, we're very clear and precise instructions. We again tell you there's 20 mgs per pump."On their very thorough manufacturing, testing, certification and validation process : "What we do is we actually contract with the CBD supplier, and we make sure that under contract, we have a guaranteed amount of CBD actually in it because the other missing link to this whole thing whether it's full spectrum or broad spectrum, how much of the CBD is actually in it. We have guarantees from our infuser, we balance these out with certified analysis, the flower, everything else on a full spectrum, but we guarantee and certify that we have 80 to 95% concentration of CBD in our full spectrum mix. So it's another level that we can guarantee to the consumer. Not only do we have all the parts of the plant, we have a guaranteed amount of CBD in it, and that's all certified. And in addition to that, I mean from a validation standpoint, you know, that's one aspect of what we do relative to the CBD, but the full panel analysis that we do is much more along a cgmp Type Certification process. Our bases for these products are manufactured in a cgmp facility. Basically that means that every aspect of the manufacturing process is validated with this double signature on all the the measurements and all the mixing steps. We then have that validated with microbiological challenge testing to make sure that that's not biologically contaminated. that then gets shipped to our CBD infuser partner who then will mix in blend in the CBD pursuant to our formulation. And then when it gets into full panel certification, we look for everything, we guarantee and certify the amount of actual CBD that's in it. We certify that it's in full compliance with the farm act, meaning that there's less than 0.3% THC in it. Then we make sure that it's full panel is none of the heavy metals and LEDs and other things. And we also do another microbiological challenge test, to make sure and confirm that in fact that there is no microbiological contamination. So when someone is getting our product, they're getting it validated from the very beginning, from the sourcing of all the raw materials that go into the base all the way to the final step, and every part in between."On the process of securing hemp: "Our hemp is sourced from one of the largest and oldest manufacturers and growers in Kentucky, which was around not just for the 2018 Farm Bill, but also for the original federal research pilot program in 2014. This is basically research grade hemp that's being grown and refined into what we believe to be and what's been demonstrated to certification to us, one of the most purest forms of CBD and hemp extract that's on the marketplace today. So just like our botanical extracts are naturally grown, every component of the formulas has been sourced from what we believe and continually verify to be the best product components available."I appreciated the thoroughness of this team in creating a product line that addresses many of the concerns and needs that people have when making a decision to try CBD. They gave meticulous detail of their phase change technology for rapid delivery and quick onset of action, the added botanicals which provide aromatherapy and synergy and contribute the the overall effectiveness of the product, their incredibly thorough testing and certification process, high quality hemp, precise dosage control, with a non greasy feel. I had the opportunity to try several of these products and will give my personal endorsement that they delivered excellent results!! As a runner who deals with chronic muscle pain, especially as I get older, the relief felt after utilizing the "be active" product eliminated the need for post run ibuprofen and other more toxic pain relievers. I highly encourage anyone out there looking for a high quality product, with some bells and whistles that you don't see for such a reasonable price, check any or all of this unique product line, asap!!"Our website is the best places to actually buy the product and to learn about the technology and the information to see about the validation, and watch the video. So we actually encourage people to go directly to the website. At the same token, we're in the process of increasing the number of retail locations that we have. And we're in the process of putting an affiliate program together as well that will basically allow us to have other people on the internet recommending the products to the friends and family."Herb Tech Pharmaceuticals Links:Website: https://herbtechpharma.com/Facebook: https://www.facebook.com/BetterOnCBD/Instagram: https://www.instagram.com/betteroncbd/Twitter: https://twitter.com/BetterOnCBDPinterest: https://www.pinterest.com/herbtechpharma/CONTACT(978) 522 0049info@herbtechpharma.com

In this episode, Laura and John from Herb Tech Pharmaceuticals join the podcast to discuss their new line of CBD topicals with Penetrating Topical CBD Phase-change Technology designed to be absorbed rapidly, provide relief and enhance your lifestyle. I enjoyed hearing about how their business got started and the passion this company has for bringing to market products that are first class. They have six different topicals, each infused with 1000 mg of CBD, and dose controlled so a pump delivers 20 mg. A bit of information how Herb Tech Pharmaceuticals came about: "Our development took a different pathway and having been focused on the pharmaceutical sector, specifically with drug delivery technology, and in fact, working with a specialty pharmaceutical development company in Boston mass that works on transdermal drug delivery technology, thought that the application of principles developed there with that technology could be directly applicable for CBD. One of the big problems obviously with CBD is that if you smoke it with all the problems with COVID-19 and everything else, obviously smoking is harmful to the lungs. If you are ingesting it, the onset of it is terrible and you've got to push the active components through the walls, into your stomach then you have first pass liver first effect and thereafter, in which case it's highly inefficient and can be problematic as well. Topicals and transdermals is could be an interesting pathway, it's safe, but you need technology to be able to get the CBD to the receptors, cannabidiol receptors. And when Laura approached me and said, Gee, is there a way we could use the technology or develop other technology that would allow us to create a company that could effectively create products to have the benefits of the CBD and other botanicals as well, but the advanced technology that allowed the CBD and the botanicals to interact much more efficiently with the human body, that's how our tech was born. We function on the same focus as really a pharmaceutical business, but just applied to CBD in botanicals." Laura goes on to explain further: "So currently, CBD, there's so many health benefits to it, we all know it. However, the side effects of current delivery methods don't overcome the benefits of the CBD. Smoking, the amount of CBD that's needed to ingest to have an effect, because again, as John mentioned, when your stomach sort of chews up most of the CBD in your stomach, you're actually getting very little into your system. And then there's a side effects of having such a high dose that you're having to take. So there's stomach upset. There's onset of action, it can have effect in 10 minutes, or it can be six hours, right? It's very unpredictable. So those two methods, they weren't efficient and they weren't working. So we said Wow, why can't we take all the wellness of botanicals, not just CBD, all herbal extracts, and really make them more effective, because we all want to use nature. But we have to be able to receive that nature, to receive it safely through the skin, which again, will not happen unless you have some sort of emulsion technology, it will just sit on the outer layer of the skin, that dead skin and it won't have an effect internally. So we're trying to create something that's only effective, but it's safe, and we've priced it so people can afford it. So I'm really excited to introduce this line of products to folks." Check out the video on our phase change technology: https://herbtechpharma.com/technology/ Laura continues with a description of their product line: "There are a lot of products out there, one size fits all, take CBD for everything. Herbal extracts can help you in certain lifestyle situations throughout your day. So we created six different need states with other botanicals infused in this delivery system. One is "be sleepy" with melatonin, chamomile, lavender "be calm" with a St. John's wort. We have "be active" with white willow, which is a precursor to aspirin, for you know to put on muscles and joints. We have a "be intimate" which is very different. And that's again infused with Red Ginseng. We have "be healthy" and to "be empowered". And again all these are not just infused with botanicals but this is something that we're very proud of. 1000 Mg's of CBD hemp extract, and again just because someone says they have 1000 mg of extract You have no idea how much CBD is in that extract. You have no idea unless the manufacturer tells you and we tell you on our labels 80 to 95% pure, and then you go to our website, there's a QR code on our bottles. We're very proud of our labels, we're very clear and precise instructions. We again tell you there's 20 mgs per pump." On their very thorough manufacturing, testing, certification and validation process : "What we do is we actually contract with the CBD supplier, and we make sure that under contract, we have a guaranteed amount of CBD actually in it because the other missing link to this whole thing whether it's full spectrum or broad spectrum, how much of the CBD is actually in it. We have guarantees from our infuser, we balance these out with certified analysis, the flower, everything else on a full spectrum, but we guarantee and certify that we have 80 to 95% concentration of CBD in our full spectrum mix. So it's another level that we can guarantee to the consumer. Not only do we have all the parts of the plant, we have a guaranteed amount of CBD in it, and that's all certified. And in addition to that, I mean from a validation standpoint, you know, that's one aspect of what we do relative to the CBD, but the full panel analysis that we do is much more along a cgmp Type Certification process. Our bases for these products are manufactured in a cgmp facility. Basically that means that every aspect of the manufacturing process is validated with this double signature on all the the measurements and all the mixing steps. We then have that validated with microbiological challenge testing to make sure that that's not biologically contaminated. that then gets shipped to our CBD infuser partner who then will mix in blend in the CBD pursuant to our formulation. And then when it gets into full panel certification, we look for everything, we guarantee and certify the amount of actual CBD that's in it. We certify that it's in full compliance with the farm act, meaning that there's less than 0.3% THC in it. Then we make sure that it's full panel is none of the heavy metals and LEDs and other things. And we also do another microbiological challenge test, to make sure and confirm that in fact that there is no microbiological contamination. So when someone is getting our product, they're getting it validated from the very beginning, from the sourcing of all the raw materials that go into the base all the way to the final step, and every part in between." On the process of securing hemp: "Our hemp is sourced from one of the largest and oldest manufacturers and growers in Kentucky, which was around not just for the 2018 Farm Bill, but also for the original federal research pilot program in 2014. This is basically research grade hemp that's being grown and refined into what we believe to be and what's been demonstrated to certification to us, one of the most purest forms of CBD and hemp extract that's on the marketplace today. So just like our botanical extracts are naturally grown, every component of the formulas has been sourced from what we believe and continually verify to be the best product components available." I appreciated the thoroughness of this team in creating a product line that addresses many of the concerns and needs that people have when making a decision to try CBD. They gave meticulous detail of their phase change technology for rapid delivery and quick onset of action, the added botanicals which provide aromatherapy and synergy and contribute the the overall effectiveness of the product, their incredibly thorough testing and certification process, high quality hemp, precise dosage control, with a non greasy feel. I had the opportunity to try several of these products and will give my personal endorsement that they delivered excellent results!! As a runner who deals with chronic muscle pain, especially as I get older, the relief felt after utilizing the "be active" product eliminated the need for post run ibuprofen and other more toxic pain relievers. I highly encourage anyone out there looking for a high quality product, with some bells and whistles that you don't see for such a reasonable price, check any or all of this unique product line, asap!! "Our website is the best places to actually buy the product and to learn about the technology and the information to see about the validation, and watch the video. So we actually encourage people to go directly to the website. At the same token, we're in the process of increasing the number of retail locations that we have. And we're in the process of putting an affiliate program together as well that will basically allow us to have other people on the internet recommending the products to the friends and family." Herb Tech Pharmaceuticals Links: Website: https://herbtechpharma.com/ Facebook: https://www.facebook.com/BetterOnCBD/ Instagram: https://www.instagram.com/betteroncbd/ Twitter: https://twitter.com/BetterOnCBD Pinterest: https://www.pinterest.com/herbtechpharma/ CONTACT (978) 522 0049 info@herbtechpharma.com

Alex Inc - need U more Vanilla Ace Ordonez - the bomb Angelo Ferreri - real deal - Marvin Aloys extended remix Block & Crown - want you all - Jackin clubmix Killed Kassette - believe it 22 weeks - you're my sunshine DJ Rose - hold on Fuse - bring out the love Lenny Fontana Keva The Diva - I don't want you back - Funkerman club remix Sharam Jey - your body - Sammy Deuce remix Alex Priston - flash mob HRDY - I feel so

Alex reviews skin care routines, your skin's importance as an organ, and how to make your skincare routine work for you. As always, if you enjoyed the show, follow us and subscribe to the show: you can find us on iTunes or on any app that carries podcasts as well as on YouTube. Please remember to subscribe and give us a nice review. That way you’ll always be among the first to get the latest GSMC Health & Wellness Podcasts.We would like to thank our Sponsor: GSMC Podcast NetworkAdvertise with US: http://www.gsmcpodcast.com/advertise-with-us.html Website: http://www.gsmcpodcast.com/health-and-wellness-podcast.html ITunes Feed : https://itunes.apple.com/us/podcast/gsmc-health-wellness-podcast/id1120883564 GSMC YouTube Channel: https://www.youtube.com/watch?v=mnvoV83f1_ATwitter: https://twitter.com/GSMC_Health Facebook: https://www.facebook.com/GSMCHealthandWellness/ Instagram: https://www.instagram.com/gsmc_health/ Disclaimer: The views expressed on the GSMC Health & Wellness Podcast are for entertainment purposes only. Reproduction, copying or redistribution of The GSMC Health & Wellness Podcast without the express written consent of Golden State Media Concepts LLC. prohibited.

The skin positivity movement is one of the best things to come out of social media. One project making major waves across Instagram right now is Epidermis by Sophie Harris Taylor. The series, which features 20 images of bare-faced women showcasing their common skin conditions including rosacea, acne and hyperpigmentation in all their glory, is a refreshing and much welcome move in the age of filters and Photoshop.   Coaching segment   We start the episode with a 5-minute coaching session where you’ll explore how to awaken to your true power on your journey to glow.   In this episode of the podcast Journey To Glow founder Caroline Frenette talks to Sophie Harris Taylor about:   The idea and movement behind Epidermis Why young women feel so much pressure to hide their skin conditions Thoughts on the way image editing apps and social media project an often unattainable type of beauty What is body and skin positivity and how to advance the movement Why the beauty industry is stuck in an illusory representation of women and the LACK of real representation in terms of beauty and skin How Sophie made the women who posed for Epidermis feel comfortable What the women thought of her photos What impact Sophie hopes Epidermis will have How we can change the beauty industry   Download the (FREE) guide Essential Radiance: Caroline's Top 5 Essential Oils For Radiant Skin journeytoglow.com/eosradiance   Download the (FREE) guide My Skin is In Crisis, Now What? journeytoglow.com/crisis   Links + resources mentioned in this episode: journeytoglow.com/38   If you like these podcast episodes, we would love your support by leaving a review on iTunes journeytoglow.com/review   Please share this episode with anyone who needs to hear this information to feel empowered on her journey to glow!   Take a screenshot of the episode you're listening, post it on social media and tag @lovemyjourneytoglow. Use the hashtag #journeytoglow or #lovemyjourneytoglow and we'll give you a shout out on the podcast!   Website journeytoglow.com Instagram instagram.com/lovemyjourneytoglow Facebook facebook.com/lovemyjourneytoglow Youtube youtube.com/journeytoglow Business Coaching & Consulting carolinefrenette.com

Originally released in July 2018, this updated encore episode focuses on the word, "echelon." Host Lynn Hickernell discusses the instances of levels throughout life. Includes "It Came From the Internet," "Wow, That's a Good Question," and a "Do Over" with family therapist Emily King about, "Epidermis." Concludes with the Miss Lynn song, "The Race." Complete show notes at https://www.patreon.com/posts/30260579/

Featuring music from Andrew Roussak, Beggars Opera, Divine Regale, Epidermis, The Gourishankar, Heads In The Sky, Initiator, Jan Dukes De Grey, Leslie’s Motel, Majesty Of Revival, Morly Gray, Protocollo C, Silentium, Steve Unruh, Transnadeznost’, and WarmRain, plus “Spotlight Sets” devoted to Argos and Ken’s Novel. Do you enjoy Prog-Scure? If so, perhaps you might consider […]

Did you ever wonder how ferns used to control their stomata? Or how plants spring back to life after being dehydrated in the desert? We got some of the answers! On top of that, we listen to pine cone disintegration, gaze at microjets from citrus peel and present our favourite plant to you!

Learn the layers of the epidermis using this mnemonic device!

This week on Boldly Boinking, we review The Disease, from Star Trek Voyager, episode 15, season 5.

In this episode, Lauren peels back the layers of your body’s largest organ. Get ready to talk connective tissues, injuries, bacteria, anthropodermic bibliopegy, and more! [Some parts not for the super-squeamish–but Julia sat through the whole recording session, so you are probably okay.] Later, enjoy* a quiz called “By the Skin of Your Teeth (Bible Trivia)”! . [* alternately, by “enjoy,” we mean “feel bad about forgetting everything you ever learned in CCD or bible study”] . . . [Music: 1) Hole, “Celebrity Skin,” 1998; 2) Frau Holle, “Ascending Souls,” 2017. Courtesy of Frau Holle, CC BY-NC 3.0 license.]

Vortragsaudio rund um das Thema Epidermis. Einige Infos zum Thema Epidermis durch dieses kurze Audio Vortrags-Podcast. Eine Ausgabe des Naturheilkunde Podcasts von und mit Sukadev Bretz, Yogalehrer bei Yoga Vidya. Anmerkung: Gesundheitliche Informationen in diesem Podcast sind nicht gedacht für Selbstdiagnose und Selbstbehandlung, sondern Gedankenanstöße aus dem Gebiet der Naturheilkunde. Bei eigener Erkrankung brauchst du … „Epidermis“ weiterlesen

26 February 2016: iona Watt of King's College London discusses her talk "Communication between the epidermis and dermis" presented at the 2015 Montagna Symposium on the Biology of Skin."

Folge 008 - Zelltypen von Pflanzen | Aufbau und Funktion Show Notes: Bitte unterstützt den Biologie Passion Podcast finanziell ➤ paypal.me/biologiepassionpdcst Hier gehts zum zugehörigen Blogartikel auf meiner Webseite. Wenn dir die Podcastfolge gefallen hat, würde mich eine kurze Bewertung auf iTunes freuen. Trag dich in meinen Newsletter ein, wenn du über neue Podcastfolgen informiert werden willst. Vielen Dank fürs Zuhören!

Track Snack is the 1st 1h Combilation between own Tracks, Loops and Samples of r.v.o. ,and presented a Live performing Mixset. Working analog with remixdeck System and Ableton connect, to create a powerful and variedly club mix.. Habitus(3),Equinox of Darkness,Quentin Terpentino,Kambium(PLEH),Kapazität +100,Kobalt(-2),Mann An (SecretMix),Power of Dark Light, Epidermis

Folge 007 - Unterschiede zwischen Sonnenblättern und Schattenblättern Show Notes: Bitte unterstützt den Biologie Passion Podcast finanziell ➤ paypal.me/biologiepassionpdcst Hier gehts zum zugehörigen Blogartikel auf meiner Webseite. Wenn dir die Podcastfolge gefallen hat, würde mich eine kurze Bewertung auf iTunes freuen. Trag dich in meinen Newsletter ein, wenn du über neue Podcastfolgen informiert werden willst. Vielen Dank fürs Zuhören!

Folge 006 - Der Aufbau des Laubblattes | Der Blattquerschnitt Show Notes: Bitte unterstützt den Biologie Passion Podcast finanziell ➤ paypal.me/biologiepassionpdcst Hier gehts zum zugehörigen Blogartikel auf meiner Webseite. Wenn dir die Podcastfolge gefallen hat, würde mich eine kurze Bewertung auf iTunes freuen. Trag dich in meinen Newsletter ein, wenn du über neue Podcastfolgen informiert werden willst. Vielen Dank fürs Zuhören!

Epidermis is a Dark&Deep Track, with hard kick drum sounds... new and in progress..! but coming soon at Beatport and many other networks... on ANCL Lincor

Prefixes are key morphemes in English vocabulary that begin words. The English prefix epi-, which means “over” or “upon,” appears in a good number of English vocabulary words, such as epidermis and epitaph. You can remember that the prefix epi- means “upon” from the noun epidemic, which is a widespread disease that comes “upon” people; you can also recall that epi- means “over” via the word epidermis, which is the outer skin that lies “over” lower layers of skin.Like this? Build a competent vocabulary with Membean.

Skin 101 Your skin is the largest organ of your body. It has 3 jobs: Protection, Regulation, Sensation Natural complexions are being viewed as more beautiful than a "tan". 3 Layers Epidermis (above skin) - waterproof, gives you your color, the layer we see Dermis-Epidermis junction - a protein layer containing collagen and elastin to give skin flexibility and stretchability Dermis - where your glands, hair follicles, and nerves are Hypodermis - (below skin) - houses adipose tissue for insulation, and blood vessels The Epidermis is made up of several separate layers based on what is happening in the cell's lifetime. The basement membrane sits right on top of the Dermis-Epidermis junction, made up of fibrous proteins to be a solid foundation. Epidermis Layers Basal Layer - Keratinocytes (makes Keratin) and Melanocytes (makes Melanin) - stem cells. Spinous Layer - Cells are actively dividing and getting squished together Granular Layer - Cells start making the proteins (keratin or melanin) that they are coded to make and it fills up all the intracellular space; the organelles of the cells get crowded out Lucid Layer (Clear) - Keratinized cells are clear; Melanin-filled cells are colored. Cells are officially dead.  Cells become coated in a hydrophobic (afraid of water) oil. Hard Layer  - cells are tightly packed together and dry; the layer we can touch. These cells are being continuously produced and shed off and replenished because the skin takes a lot of abuse. Deeper Stuff UV light from sun or tanning beds (heaven forbid!) stimulates melanocytes to divide faster and create more melanin (because melanin is reflective and keeps UV rays away from the important cells).  This is how a tan is created.  But during times of huge exposure, like tanning beds, there's not enough melanin to keep all the UV rays out so those rays can wreak havoc on the collagen and elastin proteins.  This is why over-tanned skin ages faster. Hydrating makes sure that the living and dividing cells are plump and as healthy as possible so when they move to the next layer, they are well nourished. Protection: keeps dirt and bacteria out; protections from UV radiation Regulation: releases sweat to cool the body's temp; subtly moves blood vessel closer or farther away from the surface to either cool the warm blood (like after exercise) or keep it warm (like in the winter). Sensations: allows you to feel things that touch you or come close to you. I can't seem to find any free footage of the original episode (Season 1, Episode 3) without having to sign up for a free streaming service.  The Mythbusters did a "revisit" of their Goldfinger episode.  Netflix the original, if you can. Connect with me Support us on Patreon *NEW* Join the Pharmacist Answers Podcast Community on Facebook Subscribe: iTunes, Stitcher, GooglePlay, TuneIn Radio Like the Facebook page Music Credits:  "Radio Martini" Kevin MacLeod (incompetech.com)  Licensed under Creative Commons: By Attribution 3.0  http://creativecommons.org/licenses/by/3.0/

Back from traveling I hit the ground running with tonight's podcast. I tell the happy tale of how we can keep some of our OneDrive Consumer space. Then I point you towards some good Azure AD resources. I got a Band 2 a couple of weeks ago and next I gush about how much I like it. I finish things up by talking about some patches that break Cloud Search, and some blog posts I've written recently.

Chuck Morse is joined by senior watchdog reporter for the Washington Examiner Luke Rosiak who discusses his series on the cost to the taxpayer when federal employees are "loaned" to charities. Link: http://washingtonexaminer.com/feds-version-of-workplace-giving-means-getting-paid-for-charity/article/2548568 In the second hour Chuck is joined by Bill Frady, host of Lock and Load Radio in a discussion about gun control and by Chris Putnam, author of The Supernatural Worldview. Link: http://lockandloadradio.com/ Link: http://www.supernaturalworldview.com/author/cris-putnam/

Práctica 2: La Epidermis Vegetal. Asignatura: Fisiología Vegetal. Grado en Biotecnología. Profesora: María Asunción Amorós Marco. Dpto. de Biología Aplicada. Área de Fisiología Vegetal. Proyecto PLE 2013. Universidad Miguel Hernández de Elche.

Audiovortrag zum Thema Epidermis Simple und komplexe Fakten und Meinungen rund um dieses Thema aus dem Yoga Blickwinkel von Sukadev, dem Gründer des gemeinnützigen Vereines Yoga Vidya e.V. Dieser Audiovortrag ist eine Ausgabe des Naturheilkunde Podcast. Er ist ursprünglich aufgenommen als Diktat für einen Lexikonbeitrag im Yoga Wiki Bewusst Leben Lexikon. Zum ganzheitlichen Yoga kann man auch die Theorie von Karma und Reinkarnation dazu zählen. In Ayurveda Ausbildungen erfährst du mehr zum Thema Gesundheit und Prävention. Vielleicht magst du ja deine Gedanken dazu in die Kommentare schreiben. Anmerkung: Gesundheitliche Informationen in diesem Podcast sind nicht gedacht für Selbstdiagnose und Selbstbehandlung, sondern Gedankenanstöße. Bei eigener Erkrankung brauchst du einen Arzt oder Heilpraktiker. Hier findest du: Seminare mit Sukadev Seminarübersicht Yoga Vidya YouTube Live Kanal Online Seminare Video Seminare Yoga Vidya kostenlose App Yoga Vidya Newsletter Unseren Online Shop Schon ein kleiner Beitrag kann viel bewegen... Spende an Yoga Vidya e.V.!

Metin, Metin, Metin... Henüz 13 yaşında ve yaşıtlarından çok çok daha hareketli ve yaramaz. Bulduğu her aleti kurcalayan, her şeyi tamir etmeye çalışan ve genelde başarısız olan bir çocuk Metin. Son 2 ayda 5 defa çarpılmasının ardından, neyse ki elektriğe ve prizlere olan ilgisini yitirmiş durumda. Yeni ilgisi ise sanat. En azından kendisi öyle zannediyor. Okul dersi için aldığı maket bıçağı, birkaç dakika içinde, sanata olan bu yoğun ilgisini, bütün keskinliği ile ortadan ikiye kesecek. Ve kesilen tek şey sanata duyduğu ilgi olmayacak, sol başmarmağı da bu keskinlikten nasibini alacak. Nihayetinde, Metin, 2 hafta içinde, “Yara Kabuğu” adını verdiği, çok eski bir arkadaşı ile tekrar görüşecek. Peki, bu tanışma gerçekleşene kadar içeride neler yaşanacak? Metin’in içindeki muhteşem onarım sistemleri, o yarayı nasıl kapatacak? Düşen savunma sistemi kendini nasıl koruyacak? İçeri girip bakmakta yarar var. O an Görüntüyü biraz geriye saralım ve o şanssız an’a geri dönelim. Görüntümüzde, maket bıçağının keskin ucu, kartondan kopuyor ve hızını kesmeden, yolu üzerindeki bir sonraki hedefe, Metin’in bahtsız baş parmağına doğru ilerliyor. İlk Durak Epidermis Kaçınılmaz buluşmada maket bıçağını, Metin’in derisinin en üst tabakası epidermis karşılıyor. Bu ilk tabakanın en üstü, stratum corneum adı verilen ve ölü hücrelerden oluşan katmandan oluşuyor. Hemen altında ise, önemli işlevlere sahip canlı hücreler yer alıyor. Epidermis’te bulunan hücrelerin büyük bir kısmı, ince bir protein ağı ile örülmüş durumda. Bu protein ağının temel maddesi ise keratin. Keratin, aslında, lifsi yapıda bulunan bir grup proteine verilen ad. Lif şeklinde, hücre içinde boydan boya uzandığı için bir nevi hücresel çimento görevi görüyor. Keratin, hücrelere yapısal bütünlük sağlıyor. Nitekim, Metin’in saç ve tırnaklarının bu kadar sağlam olmasının temel sebebi de keratin protein ailesi. Epidermis’in de %90‘ını keratin açısından oldukça zengin olan keratinosit adlı hücreler oluşturuyor. Diğer tüm insanlar gibi, Metin’in epidermis’i de sürekli bir devinim içinde. Ölü derinin alt kısmında yeni hücreler oluşurken, eski hücreler yaklaşık 28 gün süren bir süreçte giderek derinin dış kısmına itiliyor. Bu itilme sırasında, aşağıdaki damarlardan uzaklaştığı için ve dışarıdan gelen fiziksel darbelerle birlikte ölüyorlar. Nihayetinde, ölen hücreler deriden koparak ayrılıyorlar. Bu devinim o kadar etkili gerçekleşiyor ki, Metin her dakika 30.000-40.000 kadar hücreyi sadece derisinden kaybediyor. Kaynak: Wikimedia Epidermis’i oluşturan ölü ve canlı hücreler birbirlerine boşluk kalmayacak halde, bir yapbozu andıracak şekilde düzenlenmiş duruyorlar. Bunun yanı sıra, hücreler desmosom adı verilen hücreler arası bağlantılarla birbirlerine sıkı sıkı kenetlenmiş haldeler. Tüm bu özellikler sayesinde, Metin’in derisi büyük oranda su geçirmez halde. Bu şekilde, vücuttaki fazla su kaybı da önleniyor. Eğer, bu 1 milimetrelik katman olmasaydı, Metin ciddi oranda su kaybı yaşayıp çok kısa zamanda hayatını kaybedebilirdi. Bu tabaka, sık dokusu ile vücuda patojenlerin ve suyun girişini önleyebilse de, hızla gelen keskin bir maket bıçağına karşı yapabileceği pek bir şey yok. Bıçak, sadece 1 milimetre kalınlığındaki bu dış zırhı kolaylıkla kesip geçebilir. Nitekim, geçiyor da. Şu ana kadar, bıçağımız epidermisin hemen altındaki serbest sinir uçlarına varmadığı için, Metin’in bu kazadan henüz haber yok. Ancak, çok yakında olacak. Nitekim, bıçak yoluna devam ediyor ve karşısında, epidermisin hemen altında, onu bekleyen geniş bir sinir ağı var. Birinin canı çok yanacak. Acı yok, Acı Aslında Beyinde! Tabii canım! Bıçak, yolu üzerindeki doku ve hücreleri parçalayarak ilerlerken, normalde hücre içinde olması gereken moleküller (örneğin hücresel enerjinin transferini yapan ATP gibi) hücre dışına sızmaya ve yayılmaya başlıyor. Etrafa saçılan bu hücresel ve moleküler cesetler, bölgedeki serbest sinir uçları tarafından algılanıyor ve acıdan sorumlu sinir ...

Die vorliegende Dissertation befasst sich mit den durch S-Lost-verursachten Symptomen in der Haut, die zunächst durch starke Blasenbildung und später durch eine verzögerte Wund-heilung charakterisiert sind. Bei S-Lost handelt es sich um einen chemischen Kampfstoff, der erstmals im ersten Weltkrieg zum Einsatz kam und bis heute in vielen internationalen Kon-flikten großen Schaden anrichtete, obwohl der Gebrauch schon 1925 durch die Genfer Konvention verboten wurde. Aktuell stellt S-Lost zudem eine Bedrohung durch terroristische Aktivitäten dar. Da für S-Lost-induzierte Verletzungen bislang keine spezifisch wirksamen Behandlungs-methoden verfügbar sind, besteht großes Interesse an der Aufklärung der dem Krankheitsbild zugrunde liegenden molekularen Pathomechanismen, um daraus Rückschlüsse auf besser ge-eignete therapeutische Maßnahmen ziehen zu können. In unseren ersten Experimenten wurden als mögliche Auslöser der Blasenbildung die Expression und Sekretion ausgewählter Matrix-Metalloproteinasen (MMPs) und deren endogenen Inhibitoren, den tissue inhibitors of matrix metalloproteinases (TIMPs) in einem 3D-Haut-modell und in verschiedenen Zelltypen der Haut (Keratinozyten, Fibroblasten, mikrovaskuläre Endothelzellen, mesenchymale Stammzellen, monozytäre Zellen, PMN-Granulozyten) sowohl in Mono- als auch in Mischkultur untersucht. Unter Verwendung von molekularbiologischen und proteinbiochemischen Methoden wie qRT-PCR, Zymographie und Western Blot gelang der Nachweis, dass MMPs - insbesondere MMP-9 - nach Exposition der Zellen (v.a. Fibroblasten und monozytäre Zellen) mit S-Lost deutlich hochreguliert wurden. Zu Erhärtung der Annahme, dass MMP-9 durch Degradation der Basalmembran zwischen Epidermis und Dermis zur Blasenbildung beiträgt, konnte als Pathomechanismus nun erstmals eine parakrine Stimulation von Fibroblasten durch S-Lost-behandelte Keratinozyten identifiziert werden, als deren Folge eine vermehrte MMP-9-Sekretion resultierte. Darüber hinaus zeigte sich in weiteren Versuchen unter Verwendung des sog. Scratch-Assays und eines Transwell-basierten Invasionsassays, dass das Migrations- und Invasionsverhalten der Fibroblasten in Gegenwart des konditionierten Mediums der S-Lost-behandelten Keratinozyten positiv beeinflusst wurde. Aus klinischer Sicht sprechen diese Erkenntnisse für neue therapeutische Ansätze, die darauf beruhen sollten, die S-Lost-induzierte, auf proteolytischer Aktivität basierende Blasenbildung der Haut durch Applikation spezifischer MMP-Inhibitoren zu behandeln. In einem weiteren Projekt wurde die verzögerte Wundheilung als spätes Symptom der S-Lost-Vergiftung auf zellulärer Ebene untersucht, bei der eine eingeschränkte Re-Epithelialisierung der betroffenen Hautstellen beobachtet wird. Sowohl für den Prozess der Wundheilung als auch für die stetige Erneuerung der Haut werden epidermale Stammzellen benötigt, die für die Bildung von Keratinozyten verantwortlich sind. Diese unipotenten Progenitorzellen befinden sich in der basalen Schicht der Epidermis und sind in der Lage zu proliferieren und anschließend terminal zu differenzieren. Um eine Beeinflussung dieser Prozesse durch S-Lost zu untersuchen, wurden primäre unreife Keratinozyten (NHEK) verwendet und hinsichtlich ihres Differenzierungspotenzials untersucht. Dabei erwies sich S-Lost als potenter Induktor der Differenzierung von NHEK, was durch Bestimmung der Expression typischer Markerproteine wie Keratin-1, Involucrin und Loricrin gezeigt wurde. Die Induktion des Reifungsprozesses war sowohl von einem Rückgang der Proliferation als auch von einer verminderten Migrationsrate der Zellen begleitet. Die eingehende Analyse von mitogen-activated protein kinase (MAPK)-Signaltransduktionswegen führte zu der Erkenntnis, dass die Aktivitäten von p38 und ERK1/2 gegenteilige Rollen im Differenzierungsprozess einnehmen. Studien mit spezifischen Inhibitoren der MAPK be¬legten, dass p38 für den Reifungsvorgang in NHEK essentiell ist, während ERK1/2 diesem entgegen wirkt. So konnte durch Blockade von p38 die von S-Lost ausgelöste Differenzierung der Zellen verhindert werden. Ebenso war es durch diese Behandlung möglich, die von S-Lost stark beeinträchtige Migrationsfähigkeit der Keratinozyten wiederherzustellen, welche mit einer erhöhten MMP-1-Expression einherging. Davon abgeleitet erscheint es therapeutisch sinnvoll, selektive p38-Inhibitoren für die Behandlung von Wundheilungsstörungen nach Exposition der Haut mit S-Lost einzusetzen. Zusammenfassend erbrachten unsere Studien also den Nachweis, dass der S-Lost-induzierten Blasenbildung (als frühes Symptom) die spezifische Induktion der MMP-9 zugrunde liegt. Darüber hinaus konnte erstmals eine verfrühte Differenzierung in unreifen Keratinozyten der Haut (als mögliche Ursache für die verzögerte Wundheilung) nachgewiesen werden, wobei die MAPK p38 bei der Initiierung des Prozesses von entscheidender Bedeutung ist. Aufgrund dieser Resultate empfiehlt sich eine kombinierte Applikation von Inhibitoren der Aktivitäten von MMP-9 und p38, wobei der Einsatz jedoch zeitlich abgestimmt erfolgen sollte, um pathologische Effekte (Blasenbildung bzw. Differenzierungsinduktion in Keratinozyten) zu blockieren, ohne die positiven Auswirkungen von MMP-9 und p38 auf die Heilung (Migration von Immunzellen und Keratinozyten bzw. Reepithelialisierung) zu hemmen.

Background: Due to its strong water binding potential, hyaluronic acid (HA) is a well-known active ingredient for cosmetic applications. However, based on its varying molecular size, skin penetration of HA may be limited. Recent studies have demonstrated that low-molecular-weight HA (LMW HA) may show a certain proinflammatory activity. We thus aimed to characterize an LMW-sized HA molecule that combines strong anti-aging abilities with efficient skin penetration but lacks potential proinflammatory effects. Methods: Total RNA and total protein were isolated from reconstituted human epidermis following incubation with HAs of various molecular weights (20, 50, 130, 300, 800 and 1,500 kDa). Tumor necrosis factor-alpha expression was determined using quantitative PCR. Genonnic and proteomic expression of various junctional proteins was determined using Affymetrix and common Western blotting techniques. Results: LMW HA of approximately 50 kDa did not significantly alter tumor necrosis factor-alpha expression compared to 20-kDa HA, but revealed significantly higher skin penetration rates than larger sized HA associated with increased expression of genes and proteins known to be involved in tight junction formation and keratinocyte cohesion. Conclusion: LMW HA of approximately 50 kDa shows better penetration abilities than larger-sized HA. In addition, LMW HA influences the expression of various genes including those contributing to keratinocyte differentiation and formation of intercellular tight junction complexes without showing proinflammatory activity. These observations contribute to current knowledge on the effects of LMW HA on keratinocyte biology and cutaneous physiology. Copyright (C) 2011 S. Karger AG, Basel

Discussion of the potential of stem cells to produce skin epidermis.

Ziel der vorliegenden Dissertationsarbeit war es, die mögliche Empfänglichkeit von Karpfen (Cyprinus carpio), Koi-Karpfen (Cyprinus carpio koi) und Zebrabärblingen (Danio rerio) gegenüber der Drehkrankheit mittels Nested-PCR und histologischen Untersuchungen zu klären. Zum Vergleich wurden Drehkrankheit-empfindliche Regenbogenforellen (Oncorhynchus mykiss) mit untersucht. Für die Untersuchungen zum Eintritt von Triactinomyxon-Sporen wurden Regenbogenforellen, Karpfen, Koi-Karpfen und Zebrabärblinge exponiert und jeweils 5 Minuten, 4, 6, 8, 12 und 18 Stunden, 1, 2, 3 und 5 Tage nach der Exposition die Parasitenstadien in der Schwanzflosse und in der Haut (an der lateralen Seite) der Forellen gezählt. Bei den Regenbogenforellen wurden in den Schwanzflossen nach fünf Minuten und vier Stunden Parasitenzahlen zwischen 16 bis 25 auf einer Fläche von 1 cm² pro Hautstück (an der lateralen Seite) gefunden, während bei allen drei untersuchten Cypriniden durchschnittlich 5 intakte eingedrungene Amöboidkeimzellen pro Fisch histologisch nachweisbar waren. In der Schwanzflosse und in der Haut von Karpfen, Zebrabärblingen, Koi-Karpfen und Regenbogenforellen war zu den Untersuchungszeitpunkten 1, 4 und 8 Stunden p.e. DNA von M. cerebralis nachweisbar. Nach acht Stunden waren bei den Regenbogenforellen und bei allen drei untersuchten Cypriniden die Ambödkeimzellen noch intakt. Außerdem war eine Abnahme der Anzahl an Parasitenstadien in der Haut nur bei den Regenbogenforellen zu detektieren. Ab 8 Stunden bis 60 Tage post expositionem konnte bei Regenbogenforellen in den Schwanzflossen ein Rückgang der Infektionsintensität festgestellt werden. In der Epidermis der Schwanzflossen war die durchschnittliche Triactinomyxon-Sporen-Dichte mit Abstand am höchsten. Es konnte jedoch zum Untersuchungszeitpunkt 60 Tage bei Regenbogenforellen Parasiten-DNA mittels Nested-PCR nachgewiesen werden, obwohl diese Fische bei der histologischen Untersuchung negativ getestet wurden. Ab 50 Tage post expositionem waren nur bei den Regenbogenforellen im Kopfknorpel Entwicklungsstadien von M. cerebralis und Veränderungen in Form von Degenerationen zu finden. Alle drei untersuchten Cypriniden wiesen im Kopfknorpel keine Entwicklungsstadien von M. cerebralis oder Schädigungen auf. Nach den Ergebnissen der vorliegenden Arbeit kann somit vermutet werden, dass viele Sporoplasmen in die Epidermis der Cypriniden eindrangen, aber den Knorpel nicht erreichen konnten. Der Beweis für die erfolgte Infektion des Schädelknorpels der Regenbogenforellen mit M. cerebralis wurde anhand der klinischen und histologischen Befunde durchgeführt, während in allen drei untersuchten Cyprinidenarten, der Parasit die Haut eindrangenx , aber nicht weiter entwickeln konnte. Die aus Hautproben, Schwanzflossen und Knorpelproben der infizierten Karpfen, Koi-Karpfen, Zebrabärblinge und Regenbogenforellen gewonnene DNA wurde mittels Nested-PCR amplifiziert. Dabei stellte sich heraus, dass die Nested-PCR deutlich sensitiver war als die histologische Untersuchung mit Hämatoxylin- und Eosin- Färbung. Aufgrund der vorliegenden Ergebnisse können weiterführende Untersuchungen durchgeführt werden, um die Resistenzmechanismen der Cypriniden gegenüber der Drehkrankheit klären zu können.

Rac1 is a ubiquitously expressed member of the Rho family of small GTPases, which acts as a molecular switch by shuttling in a highly regulated manner between an active (GTP-bound) and an inactive (GDP-bound) state. Different signalling pathways, which involve integrins, growth factor receptors, cadherins as well as other Rho GTPases, can induce Rac1 activation. Only in the GTP bound form, Rac1 can associate with different effector molecules to initiate cellular responses. Initially described as an important regulator of the actin cytoskeleton, Rac1 was later found to be also involved in the modulation of other processes such as cell adhesion, proliferation, survival, differentiation and migration. In epithelial cells, Rac1 was shown to regulate the formation and maintenance of cadherin dependent cell cell contacts, which are essential for the establishment of the polarized cell morphology. Before this project was initiated, almost all knowledge about the function of Rac1 was based on in vitro studies. As constitutive deletion of the murine rac1 gene leads to early embryonic lethality, mice allowing for a conditional inactivation of the rac1 gene were generated in this study to enable the analysis of the function of Rac1 in selected tissues. To investigate the role of Rac1 in the epidermis and hair follicles and to determine its function in the establishment and maintenance of cell cell contacts between epithelial cells in vivo, mice with a keratinocyte-restricted ablation of the rac1 gene were generated and analyzed. The results obtained in this study showed that the absence of Rac1 in the murine epidermis leads to a progressive hair loss but surprisingly has no effect on the maintenance of the epidermis. The hair loss is caused by the inability of hair follicle keratinocytes to maintain their differentiation state, which leads to the phagocytic removal of the non permanent parts of the hair follicles by infiltrating macrophages. In contrast, differentiation and proliferation of epidermal keratinocytes as well as the formation and maintenance of cell-cell and cell-matrix contacts, and the deposition of the basement membrane in the epidermis are not affected by the loss of Rac1. Biochemical analysis of epidermal lysates demonstrated that the absence of epidermal defects in vivo is not a result of compensatory upregulation of closely related members of the Rho family of GTPases, further indicating that the function of Rac1 in epithelial cells in vivo is limited. Also, the analysis of the formation of the embryoid bodies from Rac1-deficient embryonic stem cells showed that the presence of Rac1 is not required for the establishment of cell cell contacts during differentiation of the polarized primitive ectoderm and for the formation of epithelial sheets, supporting the conclusion that the function of Rac1 in the regulation of cell cell adhesion between epithelial cells is dispensable. However, the re epithelialization after wounding was impaired in the mutant epidermis, demonstrating that Rac1 plays an important role in pathological conditions. The delayed wound closure in the absence of Rac1 is caused by impaired cell migration and proliferation of neo epidermal keratinocytes. Another interesting finding of this study was the observation that, in contrast to the steady state in vivo situation, isolated Rac1 deficent primary keratinocytes display severe defects in cell culture, which lead to their detachment from the matrix. While the initial adhesion is only mildly affected by the lack of Rac1, mutant keratinocytes are unable to spread, show an impaired organization of the actin cytoskeleton and fail to form mature focal adhesions. The differences between in vivo and in vitro effects resulting from the inactivation of the rac1 gene indicate that the function of Rac1 in epithelial cells depends on the complexity of the cellular system and emphasize the importance of performing in vivo studies to fully understand its role. Taken together, the data presented in this study show that Rac1 plays an important role in the maintenance of hair follicles and during epidermal wound healing, but that it is not essential for the homeostasis of the epidermis in physiological conditions and for the formation and maintenance of cell cell contacts between epithelial cells in vivo.

This week we look at new developments in the science of cancer with Fiona Watt and Andy Futreal, we will see how new gene technologies could be key to understanding cancer, and knowing the role of stem cells could be essential to find effective cures. Meanwhile, Dave tells you how to make your own electric slime. Like this podcast? Please help us by supporting the Naked Scientists

This week we look at new developments in the science of cancer with Fiona Watt and Andy Futreal, we will see how new gene technologies could be key to understanding cancer, and knowing the role of stem cells could be essential to find effective cures. Meanwhile, Dave tells you how to make your own electric slime. Like this podcast? Please help us by supporting the Naked Scientists

Die Differentialdiagnose entzündlicher Hauterkrankungen setzt seitens des Dermatologen ein hohes Maß an klinischer Erfahrung voraus. Labortechnische und histologische Untersuchungsverfahren sind dabei nur eingeschränkt hilfreich. Ausgehend von der Annahme, dass erstens das atopische Ekzem ein krankheitsspezifisches Mikromilieu in der Epidermis und Dermis aufweist und dass sich zweitens dieses spezifische Mikromilieu im Phänotyp der immunkompetenten CD1a positiven epidermalen dendritischen Zellpopulation widerspiegelt, wurde seit 1995 ein Verfahren zur Immunphänotypisierung von Langerhans Zellen und IDEC entwickelt. Es konnte gezeigt werden, dass das atopische Ekzem durch eine hohe Expression des hochaffinen IgE-Rezeptors (FceRI) bei niedriger Expression des IgG-Rezeptors FcgRII auf den dentritischen Zellen der Epidermis hoch sensitiv und spezifisch von anderen Dermatosen abgegrenzt werden kann. Ziel der vorgelegten Arbeit war es, die standardisierte Phänotypisierung CD1a positiver epidermaler Zellen so zu erweitern, dass neben dem atopischen Ekzem auch die Diagnose anderer entzündlicher Hauterkrankungen möglich wird. Es wurden 296 Patienten mittels standardisierter Phänotypisierung epidermaler dendritischer Zellen untersucht. Bei 192 Patienten konnte nach Abschluss aller klinisch diagnostischen Untersuchungen eine eindeutige Diagnose gestellt werden, so dass die an diesen Patienten erhobenen Phänotypisierungsdaten für die Analyse krankheitsspezifischer Veränderungen im Oberflächenmarkerprofil von Langerhans Zellen und IDEC herangezogen wurden. Die Patienten verteilten sich auf 8 Gruppen: Normale Haut (n=31); Atopisches Ekzem (n=59); Psoriasis (n=41); Allergisches Kontaktekzem (n=23); Kontaktekzem bei atopischer Diathese (n=14); Lichen ruber (n=6); Mycosis fungoides (n=10) und Nummuläres Ekzem (n=8). Es konnte gezeigt werden, dass der prozentuale Anteil epidermaler dendritischer Zellen in Abhängigkeit von der Diagnose variiert und dass die bei allen entzündlichen Dermatosen nachweisbare Vermehrung epidermaler dendritischer Zellen vorrangig auf eine de novo Einwanderung von IDEC in die Epidermis zurückzuführen ist. Das wesentliche Ergebnis der vorgelegten Arbeit ist die Erweiterung der diagnostischen Phänotypisierung auf die Psoriasis und das allergische Kontaktekzem. So konnte die Psoriasis durch eine Ratio von CD64/CD11b auf IDEC sinnvoll abgegrenzt werden. Das Kontaktekzem war mittels einer Ratio aus E-Cadherin/CD86 auf Langerhans Zellen zu identifizieren. Die Gruppe der Patienten mit Kontaktekzem bei atopischer Diathese wurde durch die Ratio E-Cadherin/CD80 auf IDEC erkannt. Die bereits für das atopische Ekzem publizierte Ratio FcRI/CD32 auf allen CD1a positiven Zellen wurde mit der gleichen Ratio, ermittelt nur für IDEC, verglichen. Eine Verbesserung der Sensitivität und Spezifität für die Diagnosestellung des atopischen Ekzems konnte hierdurch nicht erreicht werden. Die starke Expression von FceRI auf LC und IDEC kennzeichnet das atopische Ekzem. Typisch für das atopische Ekzem ist weiterhin die Expression von CD36 auf LC und IDEC. Die Psoriasis ist durch eine besonders starke Expression der Fcg-Rezeptoren auf IDEC gekennzeichnet. Darüber hinaus findet sich eine erhöhte passive Anlagerung von sCD23 an IDEC bei Psoriasis. Gegenüber anderen Diagnosen ist die Expression von CD11b und CD11c auf IDEC vermindert. Für das Kontaktekzem ist die im Vergleich mit anderen entzündlichen Dermatosen niedrige CD49d Expression auf LC kennzeichnend. Die Einführung neuer diagnostischer Algorithmen in die Phänotypisierung epidermaler dendritischer Zellen konnte das diagnostische Spektrum dieser Methode deutlich erweitern. Neben atopischem Ekzem und normaler Haut wurden auch Algorithmen für die Psoriasis und das allergische Kontaktekzem beschrieben. Die systematische Analyse weiterer Dermatosen mit epidermaler Beteiligung dürfte auch für diese Erkrankungen diagnostische Algorithmen aufzeigen, die das Mikromilieu der entsprechenden Krankheiten widerspiegeln. Die Phänotypisierung epidermaler dendritischer Zellen dürfte insbesondere im Rahmen klinisch-therapeutischer Studien vermehrt zur Anwendung kommen, da mit dieser Methode eine objektive, quantitative Untersuchung einzelner behandelter oder unbehandelter Hautareale im zeitlichen Verlauf möglich ist.

Die entzündlich veränderte Epidermis unterscheidet sich von der Epidermis der normalen Haut durch das Auftreten einer zweiten dendritischen, von Langerhans Zellen (LC) abgrenzbaren Zellpopulation. Während Herkunft und Funktion dieser Inflammatorischen Dendritischen Epidermalen Zellen (IDEC) noch unbekannt sind, deuten die bislang durchgeführten immunphänotypischen Untersuchungen auf eine Monozyten-abhängige Genese hin. In der vorgelegten Arbeit wurden Untersuchungen an verschiedenen epidermalen Zellsuspensionen, sowie in vitro aus Monozyten hergestellten unreifen dendritischen Zellen (MoDC) durchgeführt, um durch eine standardisierte Untersuchung weiterführende Erkenntnisse zur Immunbiologie dieser dendritischen Zellen zu gewinnen. Zunächst konnte gezeigt werden, daß die in vitro hergestellten MoDC immunphänotypisch relativ genau den IDEC entsprechen, während der Immunphänotyp der LC deutliche Unterschiede zu dem der MoDC aufweist: Während sowohl IDEC als auch MoDC stark CD36, CD11b und CLA exprimieren, sind auf LC CD36 und CD11b nur minimal und CLA deutlich weniger stark exprimiert. Außerdem konnte gezeigt werden, daß die CD1a- Expression der MoDC mit längerer Kulturdauer stärker wird, und daß eine Zugabe von autologem Serum in die Kultur diese Ausreifung von Monozyten zu MoDC hemmt. Der zweite Teil dieser Arbeit befasste sich mit vergleichenden Zellzyklusuntersuchungen von LC und Keratinozyten aus normaler und entzündlich veränderter Haut. Hierzu wurde zunächst eine Untersuchungsmethode zur gleichzeitigen Bestimmung von Zellzyklus, CD1a- Expression und Vitalität etabliert. Es wurden verschiedene Parameter wie Zellzahl, Farbstoffkonzentration, Fixierung und Vitalfärbung an einem auf den Zellinien MOLT4 und U937 basierenden Modell optimiert, und später auf epidermale Zellsuspensionen übertragen. Dieses aus einem Zellgemisch einer CD1a-exprimierenden und einer CD1a-negativen Zellinie in seinen Anteilen frei wählbare Modell erlaubte die Optimierung der Färbetechnik und wies den vermehrten DNA-Gehalt der MOLT4-Zellen nach, was auf eine chromosomale Aberration in der MOLT4-Zellinie hindeutet. Keratinozyten und Langerhans Zellen wiesen einen normalen Zellzyklus innerhalb der normalen Haut auf, was die Resultate anderer Arbeitsgruppen bestätigte. In entzündlicher Haut war die Zellteilungsrate bei Keratinozyten und epidermalen dendritischen Zellen erhöht. Die erhöhte Zellteilungsrate der epidermalen dendritischen Zellen war bislang noch nicht untersucht worden. Schließlich wurden funktionelle Untersuchungen der immunphänotypisch nachgewiesenen kostimulatorischen Moleküle durchgeführt. Hierzu wurde die antigenpräsentierende Funktion mit der gemischten Haut-Lymphozyten-Reaktion untersucht. Durch den CD86 Antikörper konnte eine T-Zell-Proliferation in vitro effektiv gehemmt werden. Zusammenfassend konnte in dieser Arbeit eine Methode zur Untersuchung epidermaler dendritischer Zellen etabliert werden. IDEC ähneln in zahlreichen Aspekten den MoDC, wogegen LC deutliche Unterschiede aufweisen. Weitere Untersuchungen am Zellzyklus entzündlicher Haut, die auf die in der vorgelegten Arbeit etablierten Methode aufbauen, werden Aufschluß über die Teilungsraten von LC und IDEC geben. Anhand der Erkenntnisse über die Rezeptorexpression auf IDEC und MoDC ist eine genauere immunbiologische Einordnung der IDEC in die Gruppe der myeloiden dendritischen Zellen wahrscheinlich.