Podcasts about viral vector

Viral vectors are a cornerstone of gene therapy and many employ experts in the viral vector services space to help design and produce their specialty vectors. These service providers are experts at making sure you get the vector you want with a titer and purity you need for your application. We're joined in this episode by Dr. Cliff Froelich, Head of Analytical Development for a viral vector services provider. Cliff and his team work with AAV, lentivirus, and other vectors to support multiple, and simultaneous, client projects. Specifically, we dive into how they use various analytical and molecular methods to monitor and assess identity, strength, purity, impurities, potency, efficiency, empty/full ratios, safety, and more. As you might expect, it's not a one-method-does-it-all approach or solution. Yes, digital PCR is in the mix here, and Cliff does a great job of outlining where it shines relative to the other methods they use regularly in their GMP practice. In our career corner portion, you'll hear about Cliff's circuitous career path, which includes stints in the poultry industry and time as a clinical dietitian. Through it all, and into his current role, Cliff brings a passion and genuine interest for the science and its potential to affect lives. Visit the Absolute Gene-ius pageto learn more about the guests, the hosts, and the Applied Biosystems QuantStudio Absolute Q Digital PCR System. 

The fields of Cell and gene therapy are booming and poised to change the treatment and prevention of disease. These research areas require the transfer of genetic material to cells, and viral vectors are commonly used here. Specifically, adeno-associated virus (AAV) and lentiviral vectors (LVV) are vectors of choice. We're joined for this episode by MinGin Kim and Kimberly Gomez, both scientists at Thermo Fisher. With backgrounds and expertise in the areas of cell and gene therapy, they help explain what all the excitement is about and how AAV and LVV are used. We hear about some of the challenges associated with viral vector work and get to hear about how digital PCR (dPCR) and good assay design are helping overcome many of these challenges to enable research and the biopharmaceutical industry. As you might expect from Absolute Gene-ius, you also get to hear their respective career path journeys and some really interesting lab stories.Visit the Absolute Gene-ius page to learn more about the guests, the hosts, and the Applied Biosystems QuantStudio Absolute Q Digital PCR System. 

In this podcast, we spoke with Margherita Neri, Director of Vector Process Development, Milan Site at AGC Biologics, Andrew Laskowski, Global Product Manager Bioreactors at Cytiva and Andreia Pedregal, Upstream Applications Specialist Manager at Cytiva about large-scale viral vector manufacturing. Our conversation included discussions around scalability, AAV (adeno-associated virus) and lentivirus production platforms, adherent culture, and next generation bioreactor improvements. I began the interview by asking Margherita about her work at AGC Biologics. She explained that as the Director of the Vector Process Development Unit, her team is responsible for process development of large scale viral vector production for gene therapy applications. Her team is also the first point of contact for new clients. Next, we talked about the types of viral vector platforms that AGC Biologics operates. Margherita described that at their Milan site, they offer AAV (adeno associated virus) and lentiviral vector production platforms in adhesion and in suspension, at 50-to-200-liter scale with expansion planned for up to 1,000 liters. I then asked her about some of the differences between adherent cell culture and suspension cell culture paths to commercial manufacturing. Margherita said that the first consideration is that most clinical trials in gene therapy have been sustained with vector produced from adherent cells, typically via processes performed using Cell Factory™ or Cell STACK®. Now that those gene therapy products are being commercialized, manufacturers need to increase scale and demonstrate comparability using a minimal comparability exercise. So, systems that allow adherent scale up are very useful in this process. Suspension processes are appealing from a scalability point of view because historically they were used for traditional protein bioproductions which can be scaled up to 20,000 – 30,000 liters. Of course, this scale still needs to be demonstrated for vector production that is performed mainly using transient transfection at 200-500 liter scale for lentivirus and between 500-to-1,000-liter maximum scale for AAV. Margherita went on to say that another important aspect in comparability between adherent and suspension systems is quality of the vector in terms of impurity profiles. She said that with adherent processes, cells are attached to the growth support, and the levels of host cell protein and cell DNA are lower when compared to suspension processes. This is very important for lentiviral vector production that is used in vivo where the requirements for impurity levels are very challenging, especially considering that for lentiviral vectors there is currently no affinity step for purification. I followed up by asking her how AGC Biologics can help customers that want to stay in adherent culture to scale up from current processes, for instance, from flatware to larger-scale production. She explained that when customers ask for a scale increase, they usually offer the iCELLis™ platform. First, they demonstrate at small scale the feasibility of the transition from flatware to the iCELLis bioreactor using the iCELLis Nano bioreactor. Using the iCELLis Nano bioreactor, AGC Biologics has developed a full upstream and downstream process that is highly representative of their process using the full-scale iCELLis bioreactor. AGC Biologics can then propose that customers use the vector produced in the iCELLis scale-down model to perform a comparability study between a clinical vector and the future commercial or large-scale vector. This comparability should be based not only on the comparison of titers, residuals, and all the CQA, but also AGC Biologics suggests performing a test of cell transduction on the target cells (i.e. CD34 or T cells) and evaluation on these cells of transfection efficiency – vector copy number, residuals and functionality. I followed up by asking Margherita about whether the iCEL...

The cell and gene therapy space poses a number of unique challenges when it comes to facility design. In this episode, Róisin McGuigan, Editor, BioInsights, speaks to Yposkesi's Louis-Marie De Montgrand and Morad El Gueddari to discuss the key considerations for designing an advanced therapy manufacturing facility that can meet the needs of future commercial demand.To read this interview and access lots more content, visit the Cell and Gene Therapy Insights website.

This episode has two parts. The first part discusses how Odylia Therapeutics is addressing rare disease, using a novel non-profit model. The second half address two rare genetic eye diseases and the approaches being designed to address them. Dr. Ashley Winslow, CEO/CSO of Odylia, describes how a non-profit is well suited to address these rare diseases, using strategies that leverage capacities in rare disease patient communities coupled to their expertise in drug discovery.  

Lisa isn't a doctor like Dr. Pepper is a doctor... Seth predicts risk taking in the history...and Scott is bad at remembering things. Lisa Keyes comes to talk about her Evo days, her first track day at Road Atlanta, getting a scholarship which ruined her and lead her to GLTC. Oh yeah... she's also an instructor and a real life Doctor. Lisa's Instagram ------ Robertson-Racing.com Track Walking Chats - Group Track Walking - Facebook Track Walking - Instagram

The tangential flow filtration (TFF) unit operation in viral vector manufacturing is a critical step on the path to commercialization. In this episode, Merck viral vector experts Ratish Krishnan and Akshat Gupta discuss best practices – and common misconceptions – when establishing process conditions and utilizing different TFF device formats.  

Four viral vector experts from diverse groups within Pall Corporation's gene therapy manufacturing organization – Denis Kole, Jon Petrone, Marc Bisschops and Nathan Hazi – join BioInsights to share insights in hot topics for the field, including optimizing upstream process yield and titer, full/empty/partially full capsid separation, and platform process development.

Saadia Zakai and Mark Schofield of Pall join Hélène Lebas of leading viral vector CDMO, Yposkesi, to discuss key current trends and challenges in AAV and lentiviral vector manufacturing, with a particular focus on the cutting edge in lentiviral vector and adeno-associated viral (AAV) vector purification tools. 

The tangential flow filtration (TFF) unit operation in viral vector manufacturing is a critical step on the path to commercialization. In this episode, Merck viral vector experts Ratish Krishnan and Akshat Gupta discuss best practices – and common misconceptions – when establishing process conditions and utilizing different TFF device formats.  

Please visit answersincme.com/KAG860 to participate, download slides and supporting materials, complete the post test, and obtain credit. In this activity, an expert on immunization discusses the mechanisms of COVID-19 vaccines, the clinical impact of viral vector–based vaccines and how to improve communication with patients regarding vaccine uptake.Upon completion of this activity, participants should be better able to: Recognize the mechanism of action of available COVID-19 vaccines; Evaluate the clinical impact of available viral vector–based COVID-19 vaccines; and Apply patient-centered counseling strategies to enhance communication with patients to improve COVID-19 vaccine acceptance.

Please visit answersincme.com/KAG860 to participate, download slides and supporting materials, complete the post test, and obtain credit. In this activity, an expert on immunization discusses the mechanisms of COVID-19 vaccines, the clinical impact of viral vector–based vaccines and how to improve communication with patients regarding vaccine uptake. Upon completion of this activity, participants should be better able to: Recognize the mechanism of action of available COVID-19 vaccines; Evaluate the clinical impact of available viral vector–based COVID-19 vaccines; and Apply patient-centered counseling strategies to enhance communication with patients to improve COVID-19 vaccine acceptance.

In this podcast, I spoke with John Ketz and Denis Kole about viral vector production, including current manufacturing challenges, navigating the road to commercialization, and successful scale up strategies. We began the interview by discussing the strides made in cell and gene therapy. Denis shared information about the several  approved therapies and the more than 2,000 ongoing clinical trials. Denis added that while the immense potential of these personalized therapies is becoming more and more clear, the challenges and bottlenecks surrounding their development and manufacturing are also becoming a reality. This is especially true when it comes to producing and delivering sufficient amounts of these complex therapies in a reasonable timeline. He explained that patient demands are increasing and the increase in IND applications for cell and gene therapies is resulting in increased demand as well as competition for resources. Access to qualified labor has become a significant bottleneck and likely will continue to remain so, at least for the foreseeable near future. He went on to say that the lack of standardized approaches for gene therapy modalities is another challenge that can increase the risk of failure. It can result in increased process complexity due to the need to screen large numbers of variables and can result in extended development times, which in turn affect the time to market for these needed therapeutics. In addition, the availability of manufacturing capacity is also becoming quite limited with the field currently reporting a significant backlog that can extend as much as 16 to 18 months. John added that he also sees capacity issues and it is something that Andelyn Biosciences® is trying to address. He also said that they are working on increasing speed and consistency. There are a lot of challenges that need to be addressed when moving from small flask or bench top scale into larger production scales. These may be challenges that you are not aware of or don't encounter at small scale. It is important to be mindful of this during scale up. ADDRESSING CURRENT MANUFACTURING CHALLENGES To continue the discussion, I asked them to share the best way to address these challenges moving forward. Denis began by sharing that the demand for clinical and commercial manufacturing for advanced therapies is expected to continue to increase as more drug development companies entering the space and new therapies continuing their development journey. As a result, the manufacturing backlog currently observed will likely continue to remain present, if not expand. So, while large pharma and a few larger biotech companies may have the resources to internally support their clinical development and manufacturing needs, most of the smaller and medium sized drug development companies will continue to face challenges associated with quick access to qualified labor and access to extensive process expertise and available manufacturing capacity. He went on to say that this is where groups like Pall's AcelleratorSM process development services and Andelyn Biosciences can play an important role with support through partnerships and collaborations. These types of collaborations really can provide the necessary resources and support for therapy developers to target shrinking the development timelines, reducing the risks associated with process development, and scaling to commercial scale. In addition, Pall and Danaher's integrated single use bioprocess offerings, provide scalable solutions that can reduce some of the risks associated with the development and manufacturing of therapeutics. These solutions really aim to alleviate some of the bottlenecks and risks associated with the lack of a standardized approach. John added that at Andelyn Biosciences, they focus on creating a robust small scale model to ensure the consistency of the product when they move into larger scale.

In this podcast, I spoke with John Ketz and Denis Kole about viral vector production, including current manufacturing challenges, navigating the road to commercialization, and successful scale up strategies. We began the interview by discussing the strides made in cell and gene therapy. Denis shared information about the several  approved therapies and the more than 2,000 ongoing clinical trials. Denis added that while the immense potential of these personalized therapies is becoming more and more clear, the challenges and bottlenecks surrounding their development and manufacturing are also becoming a reality. This is especially true when it comes to producing and delivering sufficient amounts of these complex therapies in a reasonable timeline. He explained that patient demands are increasing and the increase in IND applications for cell and gene therapies is resulting in increased demand as well as competition for resources. Access to qualified labor has become a significant bottleneck and likely will continue to remain so, at least for the foreseeable near future. He went on to say that the lack of standardized approaches for gene therapy modalities is another challenge that can increase the risk of failure. It can result in increased process complexity due to the need to screen large numbers of variables and can result in extended development times, which in turn affect the time to market for these needed therapeutics. In addition, the availability of manufacturing capacity is also becoming quite limited with the field currently reporting a significant backlog that can extend as much as 16 to 18 months. John added that he also sees capacity issues and it is something that Andelyn Biosciences® is trying to address. He also said that they are working on increasing speed and consistency. There are a lot of challenges that need to be addressed when moving from small flask or bench top scale into larger production scales. These may be challenges that you are not aware of or don't encounter at small scale. It is important to be mindful of this during scale up. Addressing Current Manufacturing Challenges To continue the discussion, I asked them to share the best way to address these challenges moving forward. Denis began by sharing that the demand for clinical and commercial manufacturing for advanced therapies is expected to continue to increase as more drug development companies entering the space and new therapies continuing their development journey. As a result, the manufacturing backlog currently observed will likely continue to remain present, if not expand. So, while large pharma and a few larger biotech companies may have the resources to internally support their clinical development and manufacturing needs, most of the smaller and medium sized drug development companies will continue to face challenges associated with quick access to qualified labor and access to extensive process expertise and available manufacturing capacity. He went on to say that this is where groups like Pall's AcelleratorSM process development services and Andelyn Biosciences can play an important role with support through partnerships and collaborations. These types of collaborations really can provide the necessary resources and support for therapy developers to target shrinking the development timelines, reducing the risks associated with process development, and scaling to commercial scale. In addition, Pall and Danaher's integrated single use bioprocess offerings, provide scalable solutions that can reduce some of the risks associated with the development and manufacturing of therapeutics. These solutions really aim to alleviate some of the bottlenecks and risks associated with the lack of a standardized approach. John added that at Andelyn Biosciences, they focus on creating a robust small scale model to ensure the consistency of the product when they move into larger scale.

In this podcast, I spoke with Hanna Lesch Ph.D., Chief Technology Officer at Exothera about viral vector manufacturing. We discussed current industry needs and challenges, scalability, end to end solutions, and key insights to a successful manufacturing and approval process. I began the discussion by asking Hanna about the viral vector manufacturing that she is working on at Exothera. She explained that Exothera is a Belgium-based CDMO delivering customized process development and GMP manufacturing services for gene therapy and viral vector-based vaccines. In 18 months, they built their new facilities with a GMP qualified manufacturing area of 2100 square meters. They have flexible manufacturing solutions for adherent or suspension scale and can accommodate small to large scale manufacturing up to 2000 L. Next, I asked if she could share her thoughts on current industry needs in terms of commercial scale production of viral vectors. She said that Exothera was founded to tackle two of the most critical challenges that manufacturers face in bringing advanced therapies to market – lack of production capacity and a shortage of bioprocessing expertise. Another area of focus is on cost of goods, as the cost of manufacturing a gene therapy product is significantly higher compared to conventional products. To reach as many patients as possible these treatments will need to be significantly less expensive. To succeed in this, Hanna believes that the industry needs to overcome several challenges, including development of standardized processes, consistent product quality, and improving manufacturing yields. She added that today there are also major time constraint risks because of delays in raw materials or consumable supply. We then discussed scalability and how Exothera manages their scale up needs. Hanna described how Exothera provides access to platforms, technical know-how and facilities to speed clients' product development time. She said that Exothera has built their capability to serve process development from their parameter screening supported by design of experiments (DOEs), small scale bioreactors for process development, middle scale for process confirmation and large scale systems to meet high yield expectations. All available in both suspension and adherent cell culture. She stated that it is key to be able to provide the full path for the product lifecycle. Then, I asked her how integrated end-to-end solutions can benefit viral vector manufacturing. She explained that end-to-end solutions can provide a fast track to clinic for their clients. This is supported by a strong technical, analytical and bioprocess know-how. These solutions also minimize the potential risks and surprises during development, providing lower cost and better predictability. She shared an example where they started a collaboration project aiming for quick AAV process scale-up into a 2000 Liter bioreactor in a very short time frame. Working with end-to-end providers, like Pall Corporation, is key to helping Exothera implement new technologies and infrastructure very quickly. She emphasized that time is money. I then followed up and asked what advice she had for others who need scale up solutions. Hanna shared that making critical decisions about the manufacturing process carefully is important, as this can drastically increase the chances of succeeding the first time. Second, select an easily scalable technology. Last, know your viral construct to avoid any surprises later and ensure regulatory compliance and a solid testing strategy for both your product and your process to achieve a straightforward approval. I closed the interview by asking Hanna if she had anything else to add for listeners. She said that if you don't have a manufacturing platform ready, it is important to find the right partner who can support your development and your manufacturing success. She also told me that she has an upcoming webinar,

In this episode Ilaria Scarfone Field Application Specialist, Pharma Analytics, Thermo Fisher Scientific speaks to Mike Brewer, Director and Global Principal Consultant, BioProduction Division, Thermo Fisher Scientific about the challenges for residual DNA testing in viral vector manufacture.To read this interview and access lots more content, visit the Cell and Gene Therapy Insights website.

Adeno-associated viral vectors, or AAVs, are the tiny shells of viruses. And today they are the most common vessels for delivering gene-based therapies.  In this episode, we'll launch into the past, present, and future of AAVs. Imagine a rocket ship blasting off from Earth with cargo bound for a distant space station, and you have a pretty good idea what adeno-associated viral vectors are all about. But instead of ferrying hardware and supplies, AAVs carry genes.It's an achievement nearly six decades in the making. That might seem like a long time to tinker with something smaller than the tiniest single-celled organism. But just like building a rocket ship destined for the deep reaches of space, the development of AAV vectors required patience, persistence, and a few leaps of faith.In the era before DNA sequencing and gene cloning, scientists in the 1960s realized that adeno-associated viral vectors could be a window into understanding genetic variations in viruses – and eventually other organisms, too.The fact that AAVs were immunologically distinct from other viruses made them curious things.So in the 1970s, AAV research took off in three directions. One determined that the simple AAV DNA could be rewritten and edited in a lab. The second found that although these small viruses can infect humans, they don't replicate without a “helper virus” (such as adenovirus). In the absence of another virus, they remain latent, and appear to be of little threat to human health. The third investigated whether AAVs could become vectors for transferring genes from one organism to another. This all culminated in 1978, when the first cloned AAV was generated and was successfully transferred to a cell of the E. coli bacterium, where it produced 50 new colonies of AAVs.So now we had proof that adeno-associated viruses could be artificially produced, that theycould be hollowed out and filled with other genetic material, and that they could potentially bea vector for delivering genes without harming their new host.By the 1980s, we had the capability to build lots of viral rocket ships and fill them with genetic cargo, we just needed a destination to send them. Enter the burgeoning field of gene therapy, with its focus on developing treatments for genetic diseases like cystic fibrosis, hemophilia B, Parkinson's, and more.Research has continued and today, adeno-associated viral vectors are a mainstay of gene therapy development. While progress is necessarily slow, gene therapy is a science that is aiming for the stars. And with AAV vectors, they are now within our reach.For more education on gene therapy, visit www.genetherapynetwork.com.

In this episode of Molecule to Market, you'll go inside the outsourcing space of the global drug development sector with Magnus Gustafsson, formerly at the time of recording Head of Global Business Development now Chief Business Officer at Biovian. Your host, Raman Sehgal, discusses the pharmaceutical and biotechnology supply chain with Magnus, covering: The advantages and disadvantages of viral vector products, including the challenge of needing to balance cost with patient access. Insights into a CDMO with a 96% staff retention rate and an enviable NPS score. The ballooning opportunity that exists in the viral vector space but also the risk that potentially comes along with such a popular platform. What makes Finland and the Nordic region so special? Magnus Gustafsson has worked in the biopharma manufacturing space for over 20 years. He holds an MSc in Biochemical Engineering, an MBA in Business Administration and a PhD in Medical Protein Science. Having worked for the likes of AZ, GE, Recipharm and Cobra Biologics, he has a track record of driving business with both commercial and strategic success combined with a customer-centric mindset.  Please subscribe, tell your industry colleagues and join us in celebrating and promoting the value and importance of the global life science outsourcing space. We'd also appreciate a positive rating! Molecule to Market is sponsored and funded by ramarketing. An international content, design and digital agency that helps companies in life sciences get noticed.

So far, about 63% of adult Indians have received both the doses of Covid-19 vaccines. And more than 90% have got at least one dose. India has majorly relied on the viral vector vaccine Covishield for its vaccination drive. Over 88% of the doses given out so far have been of Covishield, the Oxford-AstraZeneca vaccine manufactured locally under licence by the Serum Institute of India. Covaxin accounted for just a fraction. The two new vaccines approved for emergency use last month are Serum Institute of India's Covovax and Biological E's Corbevax. With last month's approval, the country now has eight vaccines in its kitty. Apart from the four discussed earlier, the remaining four are -- Sputnik V, ZyCoV-D, Moderna and Johnson and Johnson. Covishield uses the viral vector technology, while Pfizer and Moderna vaccines are based on messenger RNA or mRNA. Vaccination in western countries has been led by mRNA vaccines. Let us see what all vaccine platforms we have - Broadly, there are four types of platforms which are used in making the vaccine worldwide. They are RNA, viral vector, inactivated virus and protein-based platforms. Here, we are discussing the two most popular platforms on which most vaccines have been developed. They are viral vector and mRNA platforms. Let us understand the difference between the two, starting with the viral vector vaccine. But before that, let us understand that coronaviruses are named so because of the crown-like spikes on their surface, called spike protein or S protein. These spike proteins are the most used target for vaccines.  Viral Vector vaccines Now, let us understand what a viral vector vaccine is. It uses a harmless version of a different virus, called a “vector,” to deliver information to the body that helps it protect you. Genetic material from the target virus, in this case the Covid-19 virus, is placed inside the viral vector.  Once it enters a person's cells, it gives the cells instructions to make harmless copies of the spike protein. As the cells display the spike protein on their surfaces, the immune system starts producing antibodies and a type of white blood cells to fight off what it believes is an infection. If a person is later infected from the Covid -19 virus, these antibodies are already there to fight them. The vaccine doesn't contain the Covid -19 virus or the viral vector virus. Moreover, it does not change the DNA in any way. Covishield, Oxford-AstraZeneca, Janssen (J&J) and Sputnik V and Sputnik Lite use this platform. mRNA vaccine While mRNA vaccine uses genetically engineered messenger RNA created in a laboratory to teach our cells how to make a harmless variant of the spike protein that is found on the surface of Covid-19 virus. The presence of a foreign protein triggers a normal immune response, which produces antibodies that protects us from infection if the real virus enters the body. The vaccine is made of mRNA wrapped in a coating called the lipid nano-particle that makes the delivery easy and keeps the body from damaging it. And just like the viral vector vaccine, mRNA vaccine does not contain any virus, so it cannot give you an infection. After the mRNA delivers the instructions, our cells break it down immediately. Pfizer-BioNTech and Moderna use this technology to fight off the virus.  Watch Video

Pharmacists play an important role in emerging areas, including gene-based and cell-based therapies. Excerpts from this 2020 Midyear Clinical Meeting Presentation share how pharmacists can recommend processes for safe handling of cell- and gene-based therapies, meet standards for storage and preparation of these therapies, and prepare their departments to provide these therapies as more become available. The information presented during the podcast reflects solely the opinions of the presenter. The information and materials are not, and are not intended as, a comprehensive source of drug information on this topic. The contents of the podcast have not been reviewed by ASHP, and should neither be interpreted as the official policies of ASHP, nor an endorsement of any product(s), nor should they be considered as a substitute for the professional judgment of the pharmacist or physician.

Escaping Continuous Contagion (3) (Audio) David Eells - 11/21/21 The Apostate Leadership of God's People Removed   History must repeat.  Ecc 1:9-10  That which hath been is that which shall be; and that which hath been done is that which shall be done: and there is no new thing under the sun.  10  Is there a thing whereof it may be said, See, this is new? it hath been long ago, in the ages which were before us.   History shows that before the DS Babylon is completely taken down by Cyrus Trump, DS must take down the apostate leadership of God's people. This is because the people in Babylonish religious bondage must be set free to follow the Moses type, reformer Man-child leadership into the tribulation wilderness.  Eze 12:12-16 And the prince (This is Zedekiah, representing the apostate leadership of God's people) that is among them shall bear upon his shoulder in the dark, and shall go forth: they shall dig through the wall to carry out thereby: he shall cover his face, because he shall not see the land with his eyes. 13 My net also will I spread upon him, and he shall be taken in my snare; and I will bring him to Babylon to the land of the Chaldeans; yet shall he not see it, though he shall die there. 14  And I will scatter toward every wind all that are round about him to help him, and all his bands; and I will draw out the sword after them. 15  And they shall know that I am Jehovah, when I shall disperse them among the nations, and scatter them through the countries. 16  But I will leave a few men of them from the sword, from the famine, and from the pestilence; that they may declare all their abominations among the nations whither they come; and they shall know that I am Jehovah. While we agree that the Babylonish religious leadership is already in captivity spiritually they are still in control and that must be removed . Jer 21:4-10  Thus saith Jehovah, the God of Israel (Representing God's NT people today), Behold, I will turn back the weapons of war that are in your hands, wherewith ye fight against the king of Babylon, and against the Chaldeans that besiege you, without the walls; and I will gather them into the midst of this city. 5  And I myself will fight against you with an outstretched hand and with a strong arm, even in anger, and in wrath, and in great indignation. (This is because they have departed from the covenant as written to make up their own covenant and Jesus.) 6  And I will smite the inhabitants of this city (The apostate Jerusalem leadership), both man and beast: they shall die of a great pestilence. 7  And afterward, saith Jehovah, I will deliver Zedekiah king of Judah, and his servants, and the people, even such as are left in this city from the pestilence, from the sword, and from the famine, into the hand of Nebuchadrezzar king of Babylon, and into the hand of their enemies, and into the hand of those that seek their life: and he shall smite them with the edge of the sword; he shall not spare them, neither have pity, nor have mercy. 8  And unto this people thou shalt say, Thus saith Jehovah: Behold, I set before you the way of life and the way of death. 9  He that abideth in this city shall die by the sword, and by the famine, and by the pestilence; but he that goeth out, and passeth over to the Chaldeans that besiege you, he shall live, and his life shall be unto him for a prey. 10  For I have set my face upon this city for evil, and not for good, saith Jehovah: it shall be given into the hand of the king of Babylon, and he shall burn it with fire. (Like these two texts we still get regular warning in our morning meeting that Babylon will strike again. The Lord showed us that we could not pray away completely the effects of the DS plagues for he would use them to drain the swamp in the Church and even take some home who are innocent.)   The Alliance is slowly wiping out the DS leadership They are ratting on each other - https://realrawnews.com/  - look at each category. This has been happening for years and it is a huge job. Cyrus won battles before he took the city of Babylon. Confusion comes when people see someone in public who looks like the person arrested.  Both the DS and the Alliance are replacing these people with doubles or clones for opposite purposes.  Before Trump was elected the first time I told you he would be. Then I told you history would repeat and that he is God's modern day Cyrus, who conquered the Babylonian DS and set the people of God free from Babylonish apostasy to rebuild the Temple in Jerusalem, which is God's people.   Over 4 1/2 years ago I warned you when the Lord told us that man made plagues were coming to destroy multitudes. At that time God gave us dreams of particular enemies being killed by God in the religious and secular realm by the plagues, which hasnt happened yet, meaning something bigger is still coming.  Now the DS is losing the battle with the Alliance and are desperate to cause enough pain that the Alliance will back off their prosecution and execution of the the DS murderers. These de-populationist (eugenicists) have released worse plagues just like the ones in Revelations, which the Lord has said there is no cure for but His. /books/pdf/GV.pdf  Desperate people do desperate things, like loose more and worse plagues. I believe quarantine Lockdowns are coming again. Get supplies to stay indoors. C-19 is not the real killer. The DS population control vax is. Studies have shown Marburg, Ebola, and Aids are in the fake vax too and also ready to be released by other means too.  https://thetruereporter.com/doctor-exposes-the-truth-marburg-ebola-aids-all-is-hidden-inside-the-vaxx  https://tv.gab.com/channel/realstewpeters/view/doctor-marburg-aids-ebola-in-vaxx-618d6e68b3d7d282f9f12e9e  And the booster is 10 times more deadly than the first two jabs.  Some received placebos (saline solution) in the beginning so that the crime would not be discerned immediately.   The Death Jab James Red Pill: Dr. Vladimir Zelenko drops many proofs of the depopulation conspiracy. Very few medical experts have the courage to speak out about the DEMONIC DEMOCRATS' DASTARDLY DEPOPULATION plan. https://rumble.com/vp1a8j-breaking-news-presentation-dr.-zelenko-potus-trumps-doctor-just-risked-ever.html  Smallpox has conveniently been discovered at the CDC, which is DS to the core. This is either a distraction from the vaccine plagues above or a threat of greater things coming if the Alliance doesnt back off the arrests. https://strangesounds.org/2021/11/next-smallpox-pandemic-vials-labeled-smallpox-vaccine-research-facility-pennsylvania-video.html CIA spooks are attempting to defect to the Alliance? We know these people from our own DS faction because they have the same demons. They want to sneak back in but are not capable of repenting of witchcraft, fornication, perversion, hatred, slander, lying, and stealing demons. https://realrawnews.com/2021/11/deep-state-cia-spooks-defecting-to-white-hats/  I suggest taking them into custody and giving them a chance to tell the known truth, which will prove who they are.   On Nov 11, 2021 Missy Pollock sent this:    A Deadly Winter Glynda Lomax - 9/21/19 (David's notes in red) Prepare My children, for an intense winter comes your way and death rides on its winds. There will be much lack and hunger and need of heat. Your little ones will starve if you do not prepare. Prepare, for darkness comes your way. This is not a winter such as you have seen before, this one brings new dangers. I warn that you may prepare. Many will not heed My warning and death will claim them. This is a time to walk in much wisdom and prudence, that you may be spared from the jaws of death a little longer. Prepare for lack, for hunger and for cold. Prepare that you may stay warm in this time. Warn your loved ones. A deadly winter comes your way. Proverbs 6:6-8  Go to the ant, thou sluggard; consider her ways, and be wise: 7 Which having no guide, overseer, or ruler, 8 Provideth her meat in the summer, and gathereth her food in the harvest. Hebrews 11:7  By faith Noah, being warned of God of things not seen as yet, moved with fear, prepared an ark to the saving of his house; by the which he condemned the world, and became heir of the righteousness which is by faith. Proverbs 22:3 A prudent man foreseeth the evil, and hideth himself: but the simple pass on, and are punished. https://wingsofprophecy.blogspot.com/2019/09/a-deadly-winter.html?m=1 This message in video form: https://youtu.be/s5A8PcdsOf0 Below is more detail about what Glynda saw in the Spirit concerning the Dark Winter. She said, "This is a little more detail on the deadly winter and a couple more things I have NOT been able to shake about what is soon coming. The next two days after I made the Deadly Winter video, I kept seeing in my spirit that people were dying in their houses and no one could come and remove the bodies, ... I also saw that no police or ambulances were running. (Editors note: It is because of the fear of contagion of the Pestilences) https://m.youtube.com/watch?v=4iYxVBpDQjs    Yes, I think it will be like AA Allens vision of being sprayed from planes and killing very quickly.  Possibly paralyzing the lungs like the pestilent coral snake snow in Eve's dream.   Vision to Evangelist A.A. Allen in the 1950s  ...As I looked, suddenly from the sky I saw a giant hand reach down. That gigantic hand was reaching out toward the Statue of Liberty. In a moment her gleaming torch was torn from her hand, and in it instead was placed a cup; and I saw protruding from that great cup a giant sword, shining as if a great light had been turned upon its glistening edge. Never before had I seen such a sharp, glistening, dangerous sword. It seemed to threaten all the world. As the great cup was placed in the hand of the Statue of Liberty, I heard these words, "Thus saith the Lord of hosts, drink ye and be drunken and spue and fall and rise no more because of the sword which I will send". As I heard these words, I recognized them as a quotation from Jeremiah 25:27. I was amazed to hear the Statue of Liberty speak out in reply, "I WILL NOT DRINK!" Then as the voice of the thunder, I heard again the voice of the Lord saying, "Ye shall certainly drink". (Jeremiah 25:28) Then suddenly the giant hand forced the cup to the lips of the Statue of Liberty, and she became powerless to defend herself. The mighty hand of God forced her to drink every drop from the cup. As she drank the bitter dregs, these were the words that I heard: "Should ye be utterly unpunished? Ye shall not be unpunished, for I will call for a sword upon all the inhabitants of the earth, saith the Lord of hosts". (Jeremiah 27:29) When the cup was withdrawn from the lips of the Statute of Liberty, I noticed the sword was missing from the cup, which could mean but one thing. THE CONTENTS OF THE CUP HAD BEEN COMPLETELY CONSUMED! I knew that the sword merely typified war, death, and destruction, which is no doubt on the way. ... I saw the Statue of Liberty become unsteady on her feet and begin to stagger and to lose her balance. I saw her splashing in the gulf, trying to regain her balance. I saw her stagger again and again and fall to her knees. As I saw her desperate attempts to regain her balance and rise to her feet again, my heart was moved as never before with compassion for her struggles; but as she staggered there in the gulf, once again I heard these words: "Drink ye and be drunken and spue and fall and rise no more because of the sword which I will send among you". (Jeremiah 25:37) ...Then as I watched, another amazing thing was taking place. Far to the northwest, just out over Alaska, a huge, black cloud was arising. As it rose, it was as black as night. It seemed to be in the shape of a man's head. As it continued to arise, I observed two light spots in the black cloud. It rose further, and a gaping hole appeared. I could see that the black cloud was taking the shape of a skull, for now the huge, white, gaping mouth was plainly visible. Finally, the head was complete. Then the shoulders began to appear; and on either side, long, black arms. It seemed that what I saw was the entire North American continent, spread out like a map upon a table with this terrible skeleton-formed cloud arising from behind the table. It rose steadily until the form was visible down to the waist. At the waist the skeleton seemed to bend toward the United States, stretching forth a hand toward the east and one toward the west--one toward New York and one toward Seattle. As the awful form stretched forward, I could see that its entire attention seemed to be focused upon the United States, overlooking Canada at least for the time being. As I saw the horrible black cloud in the form of a skeleton bending toward America, bending from the waist over, reaching down toward Chicago and out toward both coasts, I knew its one interest was to destroy the multitudes. As I watched in horror, the great black cloud stopped just above the Great Lakes region and turned its face toward New York City. Then out of the horrible, great gaping mouth began to appear wisps of white vapor which looked like smoke, as a cigarette smoker would blow puffs of smoke from his mouth. These whitish vapors were being blown toward New York City. The smoke began to spread until it had covered all the eastern part of the United States. Then the skeleton turned to the west and out of the horrible mouth and nostrils came another great puff of white smoke. This time it was blown in the direction of the west coast. In a few moments' time the entire West Coast and Los Angeles area were covered with its vapors. Then toward the center came a third great puff. As I watched, St. Louis and Kansas City were enveloped in its white vapors. Then on they came toward New Orleans. Then on they swept until they reached the Statue of Liberty where she stood staggering drunkenly in the blue waters of the gulf. As the white vapors began to spread around the head of the statue, she took in but one gasping breath and then began to cough as though to rid her lungs of the horrible vapors she had inhaled. One could readily discern by the coughing that those white vapors had seared her lungs. ...As I watched, the coughing grew worse. It sounded like a person about to cough out his lungs. The Statue of Liberty was moaning and groaning. She was in mortal agony. The pain must have been terrific, as again and again she tried to clear her lungs of those horrible white vapors. I watched her there in the gulf as she staggered, clutching her lungs and her breast with her hands. Then she fell to her knees. In a moment she gave one final cough, made a last desperate effort to rise from her knees, and then fell face forward into the waters of the gulf and lay still as death. Tears ran down my face as I realized that she was dead! Only the lapping of the waves, splashing over her body which was partly under the water and partly out of the water, broke the stillness. (Editors note: This is not saying all of the US will die but Liberty will die. The lockdown and failure of supplies that will surely follow these plagues will take away Liberty. The US will recover as the DS falls.) "A fire devoureth before them, and behind them a flame burneth; the land is as the Garden of Eden before them, and behind them a desolate wilderness, yea, and nothing shall escape them". (Joel 2:3) Suddenly the silence was shattered by the screaming of sirens. The sirens seemed to scream, "RUN FOR YOUR LIVES!" Never before had I heard such shrill, screaming sirens. They seemed to be everywhere--to the north, the south, the east, and the west. There seemed to be multitudes of sirens; and as I looked, I saw people everywhere running, but it seemed none of them ran more than a few paces, and then they fell. And even as I had seen the Statue of Liberty struggling to regain her poise and balance and finally falling for the last time to die on her face, I now saw millions of people falling in the streets, on the sidewalks, struggling. I heard their screams for mercy and help. I heard their horrible coughing as though their lungs had been seared with fire. I heard the moanings and groanings of the doomed and the dying. As I watched, a few finally reached shelters, but only a few ever got to the shelters. Above the moaning and the groaning of the dying multitudes, I heard these words: "A noise shall come even to the ends of the earth, for the Lord hath a controversy with the nations. He will plead with all flesh; he will give them that are wicked to the sword, saith the Lord. Thus saith the Lord of hosts, Behold, evil shall go forth from nation to nation, and a great whirlwind shall be raised up from the coasts of the earth, and the slain of the Lord shall be at that day from one end of the earth even unto the other end of the earth. They shall not be lamented, neither gathered, nor buried; they shall be dung upon the ground". (Jeremiah 25:31-33) ...Then to my ears came another sound--a sound of distant singing. It was the sweetest music I had ever heard. There was joyful shouting and sounds of happy laughter. Immediately I knew it was the rejoicing of the saints of God. I looked, and there, high in the heavens, above the smoke and poisonous gases, above the noise of the battle, I saw a huge mountain. It seemed to be of solid rock, and I knew at once that this was the Mountain of the Lord. The sounds of music and rejoicing were coming from a cleft high up in the side of the rock mountain. It was the saints of God who were doing the rejoicing. It was God's own people who were singing and dancing and shouting with joy, safe from all the harm which had come upon the earth, for they were hidden away in the cleft of the rock. There in the cleft they were shut in, protected by a great, giant hand which reached out of the heavens and which was none other than the hand of God, shutting them in until the storm be overpassed. Missy also found this: The Next Plague Will Be Worse Glynda Lomax - 10/2/20 (Missy's notes in green) (David's Notes in Red)   I was thinking this week about how winter is coming, Covid-19 is still hanging around and that I saw another plague coming before Covid-19 is gone. And this next one is more deadly and kills faster. It keeps coming back to me, so I need to make clear what I keep seeing. This is not a “Thus saith the Lord,” this is a “I saw this and believe it is coming.” The next plague will be released by someone, it looks like, in mild weather or in an area where there is mild weather at the time. In the Spirit, I hear a hissing, like the sound an aerosol can makes, and I wondered if the virus is in a container that the person opens and just sets it down somewhere. (Missy: From what she describes it makes me think of it being sprayed from planes. This may be what Eve saw in her dream with the poisonous snakes/deadly snow falling.) This new plague will kill very quickly. I keep hearing three days. And you will SEE it on people. Some kind of sores on their upper bodies – they look an inch or so across to me, and there are lots of them. I can't see their lower bodies, I think they're wearing pants. It makes them very ill and they don't last long. This one is 100 percent released by man and done on purpose. And it has to do with blaming someone. Something about releasing it has to do with wanting to cause blame. … (The DS is releasing this but they will surely blame Trump because conservatives and forsaking the Vaccines.) Because this virus will be released on purpose and to cause blame, ... there will be no warning whatsoever when the mystery illness starts killing people. (Marburg, Aids, and Ebola will spread from the vaccinated as we have seen. But Smallpox can spread from planes.) It will spread very fast from those people. May God have mercy on His own.  Seeing this plague in the spirit again made me think of the following verse. I'm not saying this plague is a judgment –He did not say that, only that it brought this verse to mind. Take Refuge from the Coming Judgment. Isaiah 26:20 Come, my people, enter your chambers, And shut your doors behind you; Hide yourself, as it were, for a little moment, Until the indignation is past. (As in A.A. Allens saints in the cleft of the rock) Missy sent this on 11/16/21 and said, "This may be the new plague released that Glynda Lomax saw since there were sores on people. The article says that there's a concern that it could be released as as bioweapon. The vaccine for it is very harmful and would not be good to vaccinate people except in extreme cases." According to the Mayo Clinic: Smallpox is a contagious, disfiguring and often deadly disease that has affected humans for thousands of years. Naturally occurring smallpox was wiped out worldwide by 1980 — the result of an unprecedented global immunization campaign. Samples of smallpox virus have been kept for research purposes. And advances in synthetic biology have made it possible to create smallpox from published amino acid sequences. This has led to concerns that smallpox could someday be used as a biological warfare agent. No cure or treatment for smallpox exists. A vaccine can prevent smallpox, but the risk of the vaccine's side effects is too high to justify routine vaccination for people at low risk of exposure to the smallpox virus. The first symptoms of smallpox usually appear 10 to 14 days after you're infected. During the incubation period of seven to 17 days, you look and feel healthy and can't infect others. Following the incubation period, a sudden onset of flu-like signs and symptoms occurs. A few days later, flat, red spots appear first on your face, hands and forearms, and later on your trunk. Within a day or two, many of these lesions turn into small blisters filled with clear fluid, which then turns into pus. Scabs begin to form eight to nine days later and eventually fall off, leaving deep, pitted scars. Lesions also develop in the mucous membranes of your nose and mouth and quickly turn into sores that break open.   My Email to the President in 2020 David Eells Prepare for a second Plague not like the first.  I told you that Covid 19 did not fulfill all that the Lord told us three years ago that He would do with the plague to bring down the factions and apostates and that I expected more plague. Then we received this: On Jul 6, 2020, William Steenland had this dream: In this dream the Lord allowed me to follow these satanists into a very rich high rise private apartment condo in NY.  I knew that they had infiltrated the CDC and WHO and were executing a plan for their satanic New World Order. The dream changed and I was still in the same condo at a small medical facility where people were dropping like flies from some unknown pathogen or contagion. There were three women and two men who were so sick they were unable to function. I tried to grab the one woman closest to me while she was falling. In the dream I knew the elite were experimenting with the next Plandemic. I said ma'am ma'am what's wrong get up ? Again and again, but she was dead.  I went over to the nurse who was in a back room. I could tell she knew something and was divided about it. She was very sad and on her knees. I knew it was partially because she was totally helpless to assist or medicate the sick that were there. I picked her up off the ground and somewhat screamed at her ”what is happening to these people?” She told me “don't ask me that question” as she cried in fear and sadness. I yelled again for her to tell me everything you know,  what's happening to these people?! She again told me not to ask that question. She knew they were victims of a trial run for the next pandemic. In the dream I just knew it. That's is the info that kept coming to me in the dream, next plandemic and trial run experiment.    The Next Scamdemic to be Rolled Out is Marburg Virus Sent to UBM by Frances McKenzie - 10/12/21     The next scamdemic to be rolled out is Marburg. The adverse reactions to the current v@x will be blamed on a deadly outbreak of Marburg. Special jab and test are ready to be deployed. End game is depopulation. https://t.me/songsofascent/9444 Please see previous posts starting here https://t.me/songsofascent/9121     Marburg Virus Disease | CDC Marburg virus disease (MVD) is a rare but severe hemorrhagic fever which affects both people and non-human primates. MVD is caused by the Marburg virus, a genetically unique zoonotic (or, animal-borne) RNA virus of the filovirus family. The six species of Ebola virus are the only other known members of the filovirus family. Marburg virus was first recognized in 1967, when outbreaks of hemorrhagic fever occurred simultaneously in laboratories in Marburg and Frankfurt, Germany and in Belgrade, Yugoslavia (now Serbia). Thirty-one people became ill, initially laboratory workers followed by several medical personnel and family members who had cared for them. Seven deaths were reported. The first people infected had been exposed to Ugandan imported African green monkeys or their tissues while conducting research. One additional case was diagnosed retrospectively. The reservoir host of Marburg virus is the African fruit bat, Rousettus aegyptiacus. Fruit bats infected with Marburg virus do not show obvious signs of illness. Primates (including people) can become infected with Marburg virus, and may develop serious disease with high mortality. Further study is needed to determine if other species may also host the virus.  Marburg (Marburg Virus Disease) | CDC Urgent message from university hospital manager and academic Kieran Morrissey. VIDEO (freedommedia Published September 30, 2021)     COVID FADES AS HEMORRHAGIC FEVER RISES?: BEWARE covid-fades-haemorrhagic-fever-rises-beware Beware of narrow thinking. You have suffered 18 months of fake pandemic & been assaulted from every direction. Covid is being more widely exposed as fake, & there is growing awareness that the vaccines are indeed a weapon of mass destruction. As this article warns, the globalists have had the next plan in place for years & the weakened Vax population will be truly on track to follow the Great Reset, to a world population reduced by 6.5 billion peiple.   A Possible Marburg-RiVax Final Solution By Kieran Morrissey - 9/22/21 While it would now appear from Mainstream Media's propaganda and the relaxation of some of the draconian restrictions on freedom, that the war on the COVID pandemic war is being won, however I believe that this is a ruse, reinforcement part of a larger PSYCOPS to complete an ongoing Eugenics agenda. Features of the COVID-19 pandemic are very worrying as follows: – COVID-19 disease has now been overwhelmingly proven to be just another treatable cold / Influenza which was medically mis-managed by denying early treatment which caused premature deaths in very elderly and people with underlying health conditions, this gave the misconception that there were excess deaths occurring and created mass hysteria. Mainstream Media coverage of a possible Wuhan Lab virus leak heightened the fear that a perceived sudden increase in deaths could have been the result of a deadly bioweapon escape and caused further panic to develop a vaccine to stop the spread of COVID, it was stated that this would take at least 10 years further raising public fears. The use of the now debunked real-time PCR test to diagnose COVID produced large numbers of positive results, dubbed “cases” without any symptoms, i.e. asymptomatic infections which are known not to exist, and this further terrorised the population. We now know that at least 97% of those positive results were false due the excessive cycle thresholds used in testing labs. Real-time PCR was developed as a research tool and is considered not suitable for diagnostic purposes. New COVID mRNA and Viral Vector gene therapy technology “vaccine” injections were hastily developed, These injections were administered in a frenzy, in a mass global unlicensed experiment, without informed consent, causing many adverse reactions including symptoms similar to haemorrhagic fever i.e. bleeding and clots, The clots and bleeding have been found to be caused by the spike proteins generated by the body in response to the injections, and this is compounded by an undisclosed ingredient, Graphene Oxide, in the injections which is also known to cause clots and bleeding. The first and second doses of the COVID injections have now weakened the overall health of the population as can be seen by the current situation in hospitals which are well above capacity during the summer months with patients suffering adverse reactions from the injections, many patients have symptoms similar to haemorrhagic fever. The planned COVID booster injections claimed to be necessary due to waning immunity of the initial vaccinations, plus proposed injection of children, will undoubtedly cause more severe haemorrhagic fever like symptoms and also be more widespread. During my research into the false claims of excess deaths caused by COVID-19 and the pseudo-sciences which support it, I have stumbled over some very worrying developments which I will summarize due to the urgency I feel to highlight them to the public as follows: – Bill Gates GAVI published an article on 22-Apr-2021 titled “The next pandemic: Marburg?”. There have been numerous Mainstream Media articles highlighting an upcoming threat, Marburg, and referencing the WHO in recent months. Marburg Virus is a relatively rare haemorrhagic fever which was first described in 1967, there have only been a total of 376 related deaths, and only 16 deaths since 2005. Primerdesign developed a one-step Real-Time PCR test genesig® in 2018 for Marburg haemorrhagic fever. Why would they develop a test in 2018 for an illness which has not had a major outbreak since 2005? Soligenix, are currently rushing to trial a ricin-rich vaccine RiVax® for Marburg haemorrhagic fever. RiVax has Fast Track designation for the prevention of ricin intoxication by the US FDA. Approval of ricin toxin vaccine will utilize the FDA Animal Rule to eliminate the phase 1, 2 & 3 trials. Why such a rush now, to trial a vaccine for which there has only been a total of 376 deaths since 1967 and only 16 deaths since 2005? The main component of the Rivax vaccine is Ricin, this is a lectin and a highly potent toxin produced in the seeds of the castor oil plant. Soligenix shareholders include BlackRrock Fund Advisors, Goldman Sachs & Co. LLC, etc. Ricin is a lectin and a highly potent toxin produced in the seeds of the castor oil plant. Ricin is very toxic if inhaled, injected, or ingested. It acts as a toxin by inhibiting protein synthesis. It prevents cells from assembling various amino acids into proteins according to the messages it receives from messenger RNA in a process conducted by the cell's ribosome (the protein-making machinery) – that is, the most basic level of cell metabolism, essential to all living cells and thus to life itself. A paper titled Asymptomatic Infection of Marburg Virus was published by the NIH in January 2021. The fabrication of the COVID-19 pandemic and its apparent failure leads me to believe that there is a second phase or final solution at hand to catch the public while they are off-guard. Haemorrhagic fever symptoms can be easily increased and made more visible by forcing COVID booster injections on vulnerable, COVID injection injured patients, who are now prisoners in hospitals and nursing homes, plus injecting children. The haemorrhagic fever symptoms would appear as a huge Marburg pandemic and could be confirmed by the new Marburg PCR test causing mass hysteria like the world has never seen. RiVax could be quickly deployed using the existing COVID injection infrastructure and the public would be easily convinced to take it using PSYCOPS on Mainstream Media. The toxic ricin in the RiVax would kill billions of people very quickly and effectively before they realised what was happening as the deaths would be attributed to Marburg haemorrhagic fever based on the symptoms. People who refused the RiVax could be labelled as asymptomatic spreaders and placed in camps like they are doing in Australia claimed to be a COVID protection measure. If you read my previous two COVID articles you will have seen that I began this journey in early 2020 as a vaccine hesitant person. As a result of my recent study of Virology and Immunology in an attempt to understand the SARS‑CoV‑2 virus, my previously held beliefs in the theories being peddled by official science and medicine has been completely upended. I now understand that Louis Pasteur fabricated his Germ Theory of Disease in the 1850's stating that invisible microorganisms known as pathogens or “germs” lead to disease. Pasteur's ideas on Viruses were concocted upon similar theories going back to Ancient Greece and Rome, more like a developing religion or cult rather than real scientific discovery. I now believe that the theory developed by Antoine Bechamp (Pasteur bitter rival) Terrain Theory which states that microorganisms (germs) feed upon the poisonous material which they find in the sick organism and prepare it for excretion, much more similar to the natural immune response which we know to be true. However, Terrain Theory has been actively blocked due to a bias for the Germ Theory by Big Pharma due to its immense profitability. The practice of Variolation dated back to the 16th century in China and is the precursor of the first Vaccine invented for smallpox in 1796 by English physician Edward Jenner. I have discovered that the widespread use of vaccines has killed far more people than they have saved. A bookkeeper, John D. Rockefeller who made his fortune in the oil business was the first philanthropist and his foundations pioneered developments in medical research. The Rockefeller Institute for Medical Research founded in 1901, was in the forefront of research in virology and principal investigator Louis O. Kunkel had researched the biology and pathology of the mosaic virus diseases.  Beginning in 1930, the Rockefeller Foundation provided financial support to the Kaiser Wilhelm Institute of Anthropology, Human Heredity, and Eugenics, which later inspired and conducted eugenics experiments in the Third Reich. The Rockefeller empire in tandem with Chase Manhattan Bank (now JP Morgan Chase), owns over half of the pharmaceutical interests in the United States today. No alternative approach to the Germ Theory in virology, immunology or medicine including the increasing use of vaccines, has been possible due to the indoctrination of the sciences by the most powerful and wealthy industry in the world. Banking and Big Pharma who now control Mainstream Media and all medical and scientific institutions. Please do your own research, quickly, use your critical thinking and let us unite to stop this madness. I believe the Marburg-RiVax final solution could be deployed suddenly while we are relaxing after the worst of COVID and distracted by the now daily revelations on Mainstream Media regarding the Government fraud and public health mismanagement which created the COVID emergency. Watch Kieran Morrissey's full uncensored interview from the Right Now. Stream the full episode now on Ickonic.com   Marburg, Aids and Ebola in the V@X Finish naturopathic Doctor Ariyana Love says there's new research out of Spain supporting the idea that at least some batches of the coronavirus vaccine have graphene oxide in them. She tells Stew Peters that graphene oxide is included as a way to deliver nanotechnology into the blood of the vaccinated, as well as components of Ebola and the Marburg virus. Michelle Malkin has been one of the real warriors on the right over the past few years. She has a new article out: “What Every Parent Must Know About Pfizer.” She says Pfizer has a history of killing kids using the Marburg virus in vac/cines and she joins Stew to discuss the crimes the drug company got away with 25 years ago in Nigeria and is trying to get away with today.      Dr. Judy Mikovits: Antidote for Vaccine Toxin and Warns Against Dangerous Fake One Before its news - Wednesday, November 17, 2021 In this stunning interview, Dr Judy Mikovits joins Ann Vandersteel, who asks her whether COVID-19 is a real virus. Dr Judy, who worked at Fort Detrick and who worked for years with Anthony Fauci at NIH replies, “No, COVID-19, the disease is not caused by SARS-CoV-2, the coronavirus. “SARS-CoV-2 is not a real human virus, it's a monkey virus grown in the vero monkey cell line, always has been, just as SARS was created in that cell line and the variants are in that cell line. “So the disease, COVID never satisfied any of Koch's Postulates or the Hill's Criteria for a causative agent of a disease, because in order for the virus, SARS-CoV-2 to have caused COVID, everybody with evidence of infection has to have disease. “And what we know is, essentially, nobody with evidence of infection by that fraudulent PCR test – that is not testing for SARS-C0V-2 – and PCR does not test for an infectious virus. “Nobody's sick. This has a 99.7% survival rate, so there's no such thing as an ‘asymptomatic carrier' of a disease-causing virus. A virus either makes you sick or it doesn't. “And what we know is this disease called COVID was caused by contaminated flu shots – which are all contaminated with coronaviruses – and they are, every year and that those viruses recombine – the test was testing for the influenza, so this, at 4,000 people a day, in January of 2021 were dying of influenza and the CDC was labeling it, calling it “COVID”. “We know that 5G and the measures; the remdesevir, so that which is killing people by shutting down their kidneys. Everything that's been done: the masks, the shots, the isolation have caused the death and the destruction worldwide, not SARS-CoV-2.” Ann says, “So, essentially, COVID-19 is a marketing hype name to promote fear and get people running to the doctors to get these PCR tests, which are, of course, completely falsely-calibrated and they're not measuring COVID-19. So, has COVID-19 ever been isolated in a lab, then?” Dr Judy replies, “SARS-CoV-2 has been isolated from vero monkey kidney cells – in a lab, grown in –” Ann interrupts, “But not in people.” “Not from people-to-people,” Dr Judy says, “Not from a person with disease and sending it to another…It's a lab virus. All the variants are, they're sequences in a database and they always have been, since 2003 and SARS.” Ann asks, “OK, so these COVID vaccines – or what they're touting to be vaccines, these shots. I call them Clot Shots, Kill Shots. What are they, if they're not supposedly treating or preventing coronavirus?” “Well, they're synthetic, they're gene therapy, number one. They're not vaccines, they don't meet the legal or scientific definition of a vaccine, which is usually a piece of a virus or a bacteria or an ‘attenuated virus', means weakened, you took out the part that usually kills you and then you use that in a vaccine… “The simple answer is they are injecting in you a deadly synthetic virus. Synthetic virus. You are expressing the deadliest – the spike protein from HIV, the XMRV and SARS, so the three deadliest plandemics of our time. You're injecting the disease and you are shedding and spreading it, if your immune system cannot break down that synthetic lipid nanoparticle. “It's a synthetic virus. It's not a vaccine, never was, never was intended – and it's why we've been spreading the news.” Dr Judy says that the spike proteins in the flu and COVID vaccines are shed and spread and have been for years but the good news, she says is that whether you get a real virus or a synthetic virus, the treatment is the same: ivermectin, hydroxychloroquine and ozonated creams on your skin. “Keep your immune system healthy, don't ever wear a mask, because that cripples your own immune system, as you know from our book, ‘The Truth About Masks'… “The good news is, as long as people stop, right now getting any other shot, we can heal this world. Because any other shot is going to kill you and I mean a flu shot, because they have coronaviruses… “This is how they've driven this agenda for the past 30 years. They've weaponized SARS, they've weaponized Ebola – and that was 2014, when Tony Fauci released that virus on the Sierra Leone, on the Liberians, you know by way of a vaccine program. “You know, ‘We're gonna go over there and help you!' with Christian missionaries, and that's all described in our New York Times Bestseller, ‘Plague of Corruption', which came out April 14th, 2020. “I was first on the Epoch Times, April 8th, 2020, telling them all of this, showing them the Journal of Virology paper that showed hydroxychloroquine is a prophylactic treatment strategy for HIV, for SARS. And that means it's a vaccine! “Every 21 days, you got a booster shot, so the entire world could have been saved by hydroxychloroquine, just as in this book, ‘Called for Life', by Kent and Amber Brantly. He's the doctor that got infected with Ebola when Ebola was aerosolized. “And this was in the summer of 2014 and this was the cover-up by Tony Fauci of the William Thompson confession, after 15 years that MMR, giving Blacks and people of color, Hispanics, before the age of 3 had a 2-to-4 times greater risk of getting autism or severe neuro-developmental disease from that shot. “So yes, MMR in susceptible populations, three RNA viruses that'll cause autism. So what does Tony Fauci do? Oh, he released contagious Ebola on Black people, to take everybody's attention away, just like the game they played in COVID, to take everybody's attention away from the corruption… “We caught them. Not ‘we', Judy Mikovits, the Royal We: Children's Health Defense, The Highwire, the Informed Consent Action Network. Lawsuit, lawsuit, lawsuit. Proved that vaccines are not safe, have never been tested since 1989, not a single double-blind placebo controlled study, safe study has been done, as required by Federal Law, by the HHS. “That was the proof in the Fall of 2019…Was COVID started because they cloned the deadly H1N1 in 2005 – cloned – from people who died of the Spanish Flu? “It's all in here. And in our latest book, ‘Ending Plague', because we tell you the solutions are there. We tell you ozone therapy, we tell you Type 1 Interferon, we tell you Peptide T and immune modulation peptides could have stopped AIDS, could have stopped all of these diseases. “And the biggest thing our books tell you is that, ‘Never again get another injection' and you will live and we as a world will experience health.” The Public is Being Warned! Will They Try Smallpox Next or is This Another Distraction?   Vials Labeled ‘Smallpox' Found at Vaccine Research Facility in Pennsylvania The next pandemic is smallpox? - Strange Sounds     Several vials labeled “smallpox” have been found at a vaccine research facility in Pennsylvania, the US Centers for Disease Control and Prevention said Tuesday. “There is no indication that anyone has been exposed to the small number of frozen vials,” the CDC said in a statement emailed to CNN. https://youtu.be/gBmckTa4pC4 “The frozen vials labeled ‘Smallpox' were incidentally discovered by a laboratory worker while cleaning out a freezer in a facility that conducts vaccine research in Pennsylvania. CDC, its Administration partners, and law enforcement are investigating the matter and the vials' contents appear intact,” the CDC added. “The laboratory worker who discovered the vials was wearing gloves and a face mask. We will provide further details as they are available.” https://youtu.be/N2qosli3I8Q Smallpox, also known as variola, was declared eradicated in 1980 by the World Health Organization after a concerted global vaccination effort. Before that, the virus, which passes easily from person to person, infected 15 million people a year and killed about 30% of them. The last known outbreak in the US was in 1947. In 2014, employees of the National Institutes of Health found six vials of smallpox in an unused storage room as they packed up a lab at the NIH's Bethesda, Maryland, campus to move it. Two of the vials contained viable virus. The CDC said at the time there was no evidence anyone had been exposed to the contents of any of the vials. https://youtu.be/mFItWdsVnPg Governments have argued about whether to keep samples of the virus or to destroy all known copies. Most routine vaccination stopped in 1972 but military personnel and some researchers are still vaccinated. The CDC recommends that people get smallpox boosters every 3-5 years to stay protected although at least one study indicated vaccinated people may have at least some immunity for life Terri McGinley found this on twitter - November 13, 2021     BOOM! THIS IS IT. THE U.S. GOVERNMENT IS DEFINITELY GOING TO RELEASE SMALLPOX! Up to Approximately $113 Million of Oral TPOXX® Targeted for Delivery to the U.S. Government in the Fourth Quarter   Marketscreener.com - 11/04/21   - Up to Approximately $113 Million of Oral TPOXX® Targeted for Delivery to the U.S. Government in the Fourth Quarter - - Approximately $13 Million of International Sales for the First Nine Months of 2021 - - Corporate Update Conference Call Today at 4:30 PM ET November 4, 2021, 4:05 pm ET NEW YORK -- SIGA Technologies, Inc. (SIGA) (NASDAQ: SIGA), a commercial-stage pharmaceutical company, today reported financial results for the three and nine months ended September 30, 2021. "SIGA's financial results for the first nine months of 2021 include approximately $13 million of international sales, which represents a more than fourfold increase in comparison to the comparable period in 2020. Additionally, we continue to work with Meridian in a new foreign jurisdiction toward finalizing an order that would be similar in size to the initial orders received from Canada in 2020 and early 2021. The timing of the completion of the order has been and may continue to be impacted by the COVID-19 pandemic," said Phil Gomez, CEO of SIGA.  "Looking forward to the fourth quarter of 2021, we are coordinating with our supply chain to deliver up to approximately $113 million in total of oral TPOXX® to the U.S. government, almost 30% of which was delivered in October. This progress highlights the strong and growing revenue base of TPOXX, as well as its continuing importance in orthopox health security preparedness."     According to the article below, the DS had already done a mock simulation of a widespread Smallpox pandemic back in 2001, code named “Dark Winter" and now they are prepared to give the leftists a pass?? knowing that the right wing people don't trust them and won't receive the CV vaccines.  On 11-7-21, Q The Storm Rider Posted: [DARK WINTER] Now you understand why BILL GATES is TALKING about a Smallpox terror ATTACK → It's all PLANNED  The FINAL stages of the DEEP STATE PLAN are getting ready to hit.  Their Final Chess MOVES of more lockdowns, more Bioweapon release, a controlled COLLAPSE of food, gas, supply chain and weather WEAPONS are coming. https://operationdisclosureofficial.com/2021/11/07/kat-anonup-update-field-mcconnell-on-good-revelations-coming-qtsr-gene-decode-and-ron-giles/   DS Unleashes Small Pox? Missy Pollock - 10/23/19 I dreamed about something having to do with blankets. My thought in the dream was, "They are going to do something like they did to the American Indians." Then I woke up. (What came to me is the DS is going to do something that is going to appear to be helping people but instead it will be to kill people. The dream is just a parable, they aren't going to repeat what was done before by using blankets infected with Smallpox to kill people. [They could just unleash Smallpox through a more modern method upon people.])     The Vaccine Was Designed to Kill the Parents So They Could Have ALL THE CHILDREN! Reader Posted Dream on Operation Disclosure | By Scottish Castle Sent to UBM by Diane Steva - 11/13/21 (David's notes in red) Is this dream actually a nightmare? Sometimes a dream will tell you the truth that no one wants to know. The reader said, "In a recent dream, I was walking down a long, wide, and tall hallway. The floor, walls and ceiling were all stone. I ‘knew' I was traversing the third-floor long hallway from the stairs to the huge balcony overlooking the large courtyard and the fields beyond in front of this majestic Scottish style castle. I was puzzled at first about the noises I heard coming from the balcony door that was about a hundred feet in front of me. The closer I got, the louder the noise got. As I approached the doorway and stepping onto the balcony, I finally realized it was the sound of children crying, thousands of children crying. As I stepped further onto that balcony, I was awestruck by the sheer number of children I saw. I saw children crying by the tens of thousands in the courtyard and immediate fields in front of me. Then I looked to both sides and realized this castle was surrounded by hundreds of thousands of crying children, from infants to teenagers.  I then heard a voice off my left shoulder, and HE (The Lord) said, “All their parents perished from the covid vaccine. They are all alone now.” (Not only are the DS injections causing children to lose their parents but they are also going to cause a mass die off and crippling and maiming of children. There will be great suffering for all those that survive these atrocities committed by the satanist DS who are determined to sacrifice as many as they can to their god satan in exchange for maintaining their world power and dominance.) I awoke at that point, thankfully. I laid there and re-ran the whole dream through my mind a couple of times. ... In another dream last night, I was back on that balcony and the children were still weeping and I heard that voice off my left shoulder from HIM (the Lord) and He said, “The vaccine was designed to kill the parents so they could have ALL THE CHILDREN!” (Is this why they are pushing the vaccines, to GET THE CHILDREN, ALL THE CHILDREN? You know who they are. We hear about them every day. They are the “Deep State; the Satanists.” or whatever label you choose to use for EVIL.)” (The DS satanists would definitely have access to many more children in the orphanages and the foster care system because of the mass die offs. Here is an interview that Stew Peters did with a young lady in her late 20's named Ally Carter who was introduced into this system at a very young age by her mother. It is titled, “Obama and Biden Raped Me: Powerful Elites, Celebs, Demonic Sex Abuse Ring." She is speaking out about her experiences and naming high profile names.)    Witches Using Witchcraft to Spread the Plague Isaac Payne - 10/27/21 In this dream I was in some type of brick, government building about three stories high. I was translated to the second floor of this building where there were a bunch of desks and offices that didn't have walls; they were all in an open concept style. This room also joined another room that looked like a ball room used to host events.   There were women working feverishly, answering calls at the desks, and running around delivering supplies to other desks, and collaborating with the other female workers. It was utter pandemonium on the second floor. I remember phones were ringing off the hook as everyone of them were just a bunch of busy bodies. (This reminds me of Eve's dream of the workers in the kingdom working feverishly on the second floor of the "kingdom warehouse" to round up all the dogs. Isaac's dream here is showing us the flip side of that dream. The enemies of God are also working feverishly to fight against God's kingdom and His elect.) As I continued to observe, I started seeing a few of these women workers slithering like snakes from one end of the room to the other. They slithered extremely fast and in straight lines using neither their hands nor feet. What they used to propel themselves while slithering was their long, black tongues. They would lick the floor as they slithered. (The only power they have is their witchcraft words and prayers.) At this point in time they could not see me as I was invisible to them. As I observed, I began to understand why everyone was working so feverishly in a group effort. They were in distress because the plague they previously released on the world did not take affect as they had hoped, and they were trying to conjure up the strength of this plague back to its original form. However, there was nothing they could do to bring back to life their original plan of mass destruction. (Currently Biden's vaccine mandate is being struck down and ruled against by OSHA and the 5th and 6th district courts. In the state of Tennessee the Governor just passed a bill making the vaccine mandates illegal. This is beginning to happen in many other states too.) I then saw the witches divert to plan B. In a panic, they began to speak cursing over the public and then created a potion and poured the contents into a squirt bottle. (I remember seeing a glimpse of the periodic table and seeing the chemicals added together to create something called Thylutine or Toluene something in that regards I'm not sure. Whatever it was it did start with a T.) (This represents the worse plague that they are planning to replace CV-19.) I watched the witches spread out individually as each of them went to their own designated area. The began to spray many doorknobs in high traffic areas. The intent of these precise locations was because of the population density. The witches knew people would come into contact with the doorknobs and spread this conjured plague. (It has been proven that they have created the vaccines to be able to penetrate the eyes, nasal membranes and even penetrate the skin through aerosolized particles.) As I watched the contents come into contact with the doorknobs it began to instantly rust and corrode the door handles and doorknobs. Afterwards, I watched as black mold began to grow on the surfaces of everything the plague touched. The scene changed and I was back on the second floor of the building and the joy of the Lord instantaneously fell upon me in the midst of these atrocities. I began to praise, speak in tongues, and jump up and down thanking God for the day he had made and the provisions he has given. (This is how God defeated the enemies of Jehoshaphat. He told them to send the praisers out ahead of the army and then God defeated the enemies of Israel for them through the praises of His people. 2Ch 20:14-25) This time the witches could see me as they were still slithering on the floor with their black tongues. I walked up to them with great boldness and started praising God in front of them.  I was saying, “This is the day the Lord has made! He is God and God alone!” I shouted many other praises in front of the witches. They instantly stopped their slithering and looked at me with total confusion.  One of the witches stood before me in shock. She had black purplish hair, pale skin and was wearing a long black dress, and a black pointed witch's hat. At this point, all their evil, feverish work halted. The entire floor of witches stood confounded and halted in their progress they had made spreading this potion or plague. (There is an angel named Jeruel who stands above me in the balcony at my house and he too goes out and fights for UBM with our praises.) I walked up to the head witch and began to praise God in front of her. She couldn't slither anymore because she was confused and her attention was on me. She had blonde hair that seemed dyed, pale skin, pale blue eyes, a long black tongue, and wearing all black garments. She seemed to be younger; in her late 20's.  I looked at her and said, “Blessed be the God of all the universe! The earth is his footstool!” I was shouting and clapping; jumping up and down and smiling as His peace was upon me surpassing all understanding. She began to yell and argue with me saying, “Your God is not more powerful than mine! No, he's not!” She was incoherently babbling the same things over and over.  I looked right at her and said, “My God created your god and at no point did He intend to share his power with your god! My God is Sovereign, and the beginning and the end has already been fulfilled! Your god has already been judged!” Then I said one last thing to her, “You will submit to Jesus!” She couldn't even function for disbelief, confusion, and amazement. (Their god is satan and he has already been defeated.) The second floor of witches could not pick up where they left off conjuring their evil plague because I was praising God uncontrollably! The praising of God also fixed all the doorknobs that were saturated with this potion and all door knobs became as brand new. Praising God halted all their wicked plans! Then I woke up. (Let's remember the power that is in our praises! The demons hate true praise that glorifies God. It will put them to flight every time!)   Be Anxious for Nothing Angelica Garza - 10/29/21 I felt to share what the Lord was saying to me when I was finishing up on a Word study on anxiety, He said, …Don't allow Satan to get you to be anxious. He wants to draw you in different directions, so that your heart, mind, will, and emotions are divided from God. Satan does not want you to give your full attention to our relationship, and your purpose. The time is short, and he will use your emotions to prevent you from bearing fruit, and from having faith and believing the truth of my word. If you're looking  at your emotions, (moved by them) you are walking by sight, and you will be blind. You will be unable to have peace, and unable to enter into my rest. You will always be double-minded, and unable to receive from me. This will ultimately cause you to be drawn away from me, alienated from my life, and be counted as an unbeliever. It is of the utmost importance that you be continually in prayer. Asking me for all that I said I would provide for you. For I care for you. I desire to have communion with you, to interface, to connect with you my daughter. I desire to hear your heart, and answer your requests. There is no shame in asking me for everything. I am the provider and sustainer of all life, and of my children. I desire that you show me thankfulness, for my great love for you. Praise me, worship me, I am your sustainer, I AM Supreme! Humble yourself as my creation. The creation cannot sustain itself. Cry out to me, and I will give you the life that you desire in me. Without me, all things are meaningless, and without purpose. I AM your life; I AM your breath. When you do this, your life will have meaning again. It will be whole and undivided.  Your mind will be sound, and you will not fear! You will be fully assured when you wake, when you sleep, when you go out, when you come in. No weapon formed against you shall prosper. No curse can come upon you. You will have perfect peace, when your mind is solely stayed on me. You will be confident in the knowledge of your salvation, and in the giftings I have given you. You will be content in all things, you will want for nothing, your will be filled by my spirit continually! You will be fully assured that I have called you, and of where you are, and of who you are. You will find your place in the body, and as my daughter (my son). You will prosper in all that you do, so long as you are looking to me to sustain you, to keep you.  The peace that you so desire, surpasses, and is above everything you can see with your eyes, and what you can feel with your senses and emotions. It is real, it is tangible (to the spirit man), it surrounds the one who trusts me beyond their own ability to comprehend what I have planned. I said, “lean not on your own understanding” because it is weak, and limited. I gave you your mental capacity, and designed it. Should not the creation submit to its designer, it creator? My grace is sufficient for you. Your weakness, is my Glory. I am glorified in your weakness. If you had no weakness, you would not need me. How soon am I forgotten by my children when they have strength. Glory in your weaknesses, for in it, my power is shown to all! Guard your heart. Guard your thoughts, mind, submit your will, and emotions to me. No don't exchange my truth for lies. Satan wants to invade your mind, and destroy the fruit that my Son paid for you to have. Is it not precious? Is not my nature longed for and most beloved? Does it not bring peace? Does it not bring joy? There is none like me! Should it not be guarded with truth, and faith? Fight the good fight of faith, and lay hold of life eternal. War against the enemy, and do not submit your mind, will and emotions to him, or he will become your master. If you believe me at my word, then you will receive my peace, you will not be distracted with the things that I said I would do for you, if you let me. In my Son, is peace. He is the Prince of Peace, my beloved Son. Stay connected to the Vine. Please prepare yourselves, friends and family for this plague. We make zero money on these books. Learn from Free God's Vaccine E-Book, How to be Protected Also you can order it in print here: httpss://www.ubm1.org/?page=ubmbooks Listen to the Audio Teachings “God's Vaccine” Parts 1-6:

Welcome to Imagine the Possibilities with Intellia, where we showcase Intellia talent across departments. On this show, we take deep dives into all things culture and careers. We talk about career steps (and missteps), development, growth, and more. Today we are imagining the possibilities with Francesca Vitelli, Vice President Viral Vector and Cell Therapy Process Development. Just imagine the possibilities of what we can learn together! This podcast is intended to provide individuals who are pursuing a career in the biopharmaceutical industry an opportunity to learn from Intellia employees about our industry and potential career paths. The views and opinions expressed represent those of the participating individuals and do not necessarily represent the views or opinions of Intellia Therapeutics or endorsement by Intellia Therapeutics.

Pharmacy Times Continuing Education (PTCE) provides industry leading pharmacy CE to retail, oncology, managed care, specialty and health-systems pharmacists. They use multiple deliverables in the live, virtual, on-demand, and print formats created by in-house pharmacists to deliver tailored multi-specialty education. Adverse Effects versus Convenience of the COVID-19 Viral-Vector Vaccines Educational Objectives: Articulate the benefits of viral-vector vaccines compared with the risk of thrombosis with thrombocytopenia syndrome (TTS) Elaborate on current viral-vector TTS adverse effect incidence data and risk among patients who have received or will receive a viral-vector vaccine  Faculty: James Lewis, PharmD, FIDSA ID Clinical Pharmacy Supervisor Oregon Health & Sciences University Portland, Oregon James Lewis, PharmD, FIDSA, has no financial relationships with commercial interests to disclose. Pharmacy Times Continuing Education™ is accredited by the Accreditation Council for Pharmacy Education (ACPE) as a provider of continuing pharmacy education. This activity is approved for 0.5 contact hours (0.05 CEU) under the ACPE universal activity number 0290-0000-21-264-H06-P. The activity is available for CE credit through June 29, 2022. This activity is supported by an educational grant from Janssen Therapeutics, Division of Janssen Products, LP. See omnystudio.com/listener for privacy information. Learn more about your ad choices. Visit megaphone.fm/adchoices

Pharmacy Times Continuing Education (PTCE) provides industry leading pharmacy CE to retail, oncology, managed care, specialty and health-systems pharmacists. They use multiple deliverables in the live, virtual, on-demand, and print formats created by in-house pharmacists to deliver tailored multi-specialty education. Adverse Effects versus Convenience of the COVID-19 Viral-Vector Vaccines Educational Objectives: Articulate the benefits of viral-vector vaccines compared with the risk of thrombosis with thrombocytopenia syndrome (TTS) Elaborate on current viral-vector TTS adverse effect incidence data and risk among patients who have received or will receive a viral-vector vaccine Faculty: James Lewis, PharmD, FIDSA ID Clinical Pharmacy Supervisor Oregon Health & Sciences University Portland, Oregon James Lewis, PharmD, FIDSA, has no financial relationships with commercial interests to disclose. Pharmacy Times Continuing Education™ is accredited by the Accreditation Council for Pharmacy Education (ACPE) as a provider of continuing pharmacy education. This activity is approved for 0.5 contact hours (0.05 CEU) under the ACPE universal activity number 0290-0000-21-264-H06-P. The activity is available for CE credit through June 29, 2022. This activity is supported by an educational grant from Janssen Therapeutics, Division of Janssen Products, LP. See omnystudio.com/listener for privacy information. Learn more about your ad choices. Visit megaphone.fm/adchoices

Pharmacy Times Continuing Education (PTCE) provides industry leading pharmacy CE to retail, oncology, managed care, specialty and health-systems pharmacists. They use multiple deliverables in the live, virtual, on-demand, and print formats created by in-house pharmacists to deliver tailored multi-specialty education. Adverse Effects versus Convenience of the COVID-19 Viral-Vector Vaccines Educational Objectives: Articulate the benefits of viral-vector vaccines compared with the risk of thrombosis with thrombocytopenia syndrome (TTS) Elaborate on current viral-vector TTS adverse effect incidence data and risk among patients who have received or will receive a viral-vector vaccine  Faculty: James Lewis, PharmD, FIDSA ID Clinical Pharmacy Supervisor Oregon Health & Sciences University Portland, Oregon James Lewis, PharmD, FIDSA, has no financial relationships with commercial interests to disclose. Pharmacy Times Continuing Education™ is accredited by the Accreditation Council for Pharmacy Education (ACPE) as a provider of continuing pharmacy education. This activity is approved for 0.5 contact hours (0.05 CEU) under the ACPE universal activity number 0290-0000-21-264-H06-P. The activity is available for CE credit through June 29, 2022. This activity is supported by an educational grant from Janssen Therapeutics, Division of Janssen Products, LP. See omnystudio.com/listener for privacy information.

In today's episode we're talking all about the vaccine and your cycle. Before you listen to this episode, make sure you're okay with hearing the truth.  Feel free to take what you need and leave what you don't.  Things we discuss: Conventional vaccines vs. the mRNA, Viral Vector, & Protein Subunit S Proteins and what they mean Why we need to believe women  Potential solutions for supporting your body Voluntary Research Study: https://redcap.healthinstitute.illinois.edu/surveys/index.php?s=LL8TKKC8DP mRNA: https://www.genome.gov/genetics-glossary/messenger-rna Different Types of Vaccines: https://www.gavi.org/vaccineswork/there-are-four-types-covid-19-vaccines-heres-how-they-work#:~:text=The%20four%20main%20types%20of,RNA%20and%20DNA) Understanding Spiked Protein:- https://pubmed.ncbi.nlm.nih.gov/33053430/- https://www.nature.com/articless41401-020-0485-4#:~:text=The%20S%20protein%20is%20the,protective%20immunity%20against%20viral%20infection  Connect with Me Here: Period Nutrition Plan  Ready to learn how to work with your monthly flo rather than against it? Enroll in Optimize Your Flo today! Favorite Supps for Managing Cramps: My Period Tea & Period Pain Killer Semaine Use BERRI20 for a discount Did you love this episode? Leave a review and share on the 'gram with your friends! Speaking of friends, want to connect? Instagram - @berrionlberry

American health regulators have paused the use of the Johnson & Johnson coronavirus vaccine, following reports of rare blood clotting in six recipients. Like the AstraZeneca vaccine, Johnson & Johnson is what's known as a viral vector vaccine - where another virus is used like a taxi to deliver a bit of coronavirus to help teach the immune system. So on today's Coronacast, what are viral vector vaccines and could the rare blood-clots be a problem with them more broadly? Also on today's show: * What do the data show regarding the risks of clotting for people over the age of 50? * What can we learn from the US vaccine rollout? (We hear from special guest Anthony Fauci, who Tegan interviewed as part of an event presented by UNSW)

Gene therapy is promising to provide treatments and potential cures for a long list of rare, genetic diseases. A key element of these therapies are the viral vectors that are used to deliver and insert the genetic material used to treat a patient. Guangping Gao, co-director of the Li Weibo Institute for Rare Diseases Research, director of the Horae Gene Therapy Center and Viral Vector Core, and professor at the University of Massachusetts Medical School; and Phillip Tai, assistant professor at the University of Massachusetts Medical School, discuss a recent review article that they co-authored in Nature's journal Signal Transduction and Targeted Therapy that looks at viral vector platforms for gene therapy. We spoke to the researchers about viral vectors, the role they play in gene therapy, and the decision process that goes into the selection of a vector of a specific gene therapy. Thanks to Pfizer, Inc., Bluebird, and Novartis Gene Therapies for their support of this podcast, part of our Platforms of Hope: Advances in Gene Therapy and Gene Editing series.

Thank you for joining us again for episode 10 of COVID Conversations. This series is a special presentation of OccPod, the official podcast of the American College of Occupational and Environmental Medicine. ACOEM is dedicated to sharing the latest COVID-19 news, and host conversations grounded in science with a focus on OEM, or occupational and environmental medicine. In today's episode, Erin and Dr. Nabeel discuss viral vector vaccines, and the differences between current inoculations offered in the United States. With President Biden's '100 million doses in the first 100 days' promise met ahead of schedule, Erin and Dr. Nabeel break down the unique attributes of what makes these vaccinations successful against COVID-19. Produced and recorded in tandem with ACOEM's COVID-19 Q&A Forum, these recordings aim to educate a general audience on SARS CoV2 and COVID-19. Be sure to check back soon for a new episode!

EP214.1 10 Technologies to Watch ภาค 1 แบ่งปันการเข้าร่วมงาน Thailand Tech Show 2020 งานแสดงเทคโนโลยีและนวัตกรรมครั้งใหญ่ ในรูปแบบ Online Event จัดโดยสำนักงานพัฒนาวิทยาศาสตร์และเทคโนโลยีแห่งชาติ (สวทช.) กระทรวงการอุดมศึกษา วิทยาศาสตร์ วิจัยและนวัตกรรม (อว.) เพื่อขับเคลื่อนเวทีต่อยอดผลงานวิจัยพร้อมใช้จาก สวทช. และพันธมิตรหน่วยงานวิจัยทั้งภาครัฐและเอกชน สถาบันการศึกษาจากทุกภาคของประเทศ ในรูปแบบตลาดเทคโนโลยี ในวันที่ 2 ธันวาคม 2563 เวลา 9.15 – 10.00 น. ภายใต้แนวคิด “วิถีชีวิตใหม่ นวัตกรรม เพื่อการลงทุน” สำหรับเนื้อหาที่ฟังและนำมาส่งต่อ หัวข้อเรื่อง “10 เทคโนโลยีที่น่าจับตามองสำหรับธุรกิจ” (10 Technologies to Watch) โดย ดร.ณรงค์ ศิริเลิศวรกุล ผู้อำนวยการสำนักงานพัฒนาวิทยาศาสตร์และเทคโนโลยีแห่งชาติ (สวทช.) - กระทรวงการอุดมศึกษา วิทยาศาสตร์ วิจัยและนวัตกรรม 10 เทคโนโลยีที่น่าจับตามองสำหรับธุรกิจ (10 Technologies to Watch) ใน EP นี้จะฟังเฉพาะหัวข้อ 1-4 ดังนี้ Thailand Tech Show 2020 >> 10 Technologies to Watch 1. วัคซินโควิด-19 (COVID-19 Vaccines) 1.1 KILL VIRUS 1.2 PROTEIN-BASED 1.3 NUCLEIC ACID 1.4 VIRAL VECTOR (4 เทคโนโลยีที่ผลิตในปัจจุบัน) 2. ยาแก้ไขความชรา (Rejuvenating Drug) >> การทำงานของยาอายุวัฒนะมณีแดง (REDGEMs) 3. อินเทอร์เน็ตของสรรพสิ่งสุขภาพ (Internet of Health Things : IoHT) >> Wearable Medical Device + Remote Patient Monitoring ผ่าน IoHT 4. ชิปสายพันธุ์ใหม่ (Neuromorphic Chip) ฟังได้ที่ youtube - https://youtu.be/bqJeX2L_XbM Podcast - https://anchor.fm/ratree อ่านเรื่องราวที่ https://www.facebook.com/girlone2020 *** บันทึกเสียง 2/12/2020 ***[6/12/2020] อ้างอิง https://www.tts2020-online.com/agenda --- This episode is sponsored by · Anchor: The easiest way to make a podcast. https://anchor.fm/app Support this podcast: https://anchor.fm/ratree/support

On today's show Dr. Drew Colfax runs the local Coronavirus numbers and our local response to the new curfew. He also answers listener questions about differences between the vaccines, testing on the south coast, intranasal vaccines, and the viral vector method.

We discuss how an RNA therapy that’s delivered by a viral vector may hold potential to treat SMA. Also, SMA News Today’s forums moderator, DeAnn Runge discusses how airlines do not allow wheelchair users to stay in their chairs during the flights. She describes how vulnerable it feels being separated from your chair during airline travel. Are you interested in learning more about the latest treatment for spinal muscular atrophy? Visit TreatSMA.com to see how this treatment works, hear about family stories, and learn about the steps to starting treatment. Visit TreatSMA.com.

In this podcast, we interviewed Dr. Mats Lundgren, Ph.D. Customer Applications Director, Life Sciences, GE Healthcare, Sweden about developing platform processes for viral vector manufacturing and Mats describes a recently developed platform process for adenovirus production.  View show notes at cellculturedish.com/viral-vector-manufacturing-for-gene-therapy-developing-a-platform-process/

Dr. Stephen Russell discusses his group's recent publication in Lancet (http://www.thelancet.com/journals/lancet/article/PIIS0140-6736(17)31868-8/fulltext) regarding successful phase 3 trial results of viral vector RPE65 gene therapy, the first such successful trial in medicine.

In this podcast, we were fortunate to have a group of experts for a panel discussion on vaccine manufacturing and viral vector production. Interviewees: Hanna P. Lesch, PhD, Research and Development Director at Finvector Vision Therapies Jose Castillo, PhD, Co-Founder and CTO of Univercells Mario Philips, Vice President and General Manager, Single-Use Technologies, Pall Life … Continue reading Enabling Vaccine Manufacturing and Viral Vector Production using the iCELLis – a single-use, automated, and closed manufacturing platform →

New vectors for successful transgene delivery in patients are more than needed. Current gene therapeutic vectors are mostly based on virus genomes. A main reason is the very efficient administration of the vector into the target cells. The viral background of these systems also has severe side effects like e.g. immunological reactions of the patient or the activation of oncogenes due to insertional mutagenesis, which can result in cancer development. A possibility to overcome these problems is the establishment of extrachromosomally maintained, autonomously replicating and non-viral vectors. The goal of this work was to establish such a novel system with the help of the CENH3 protein. The centromere-specific histone 3 variant CENH3 (H.sapiens: CENH3CENP-A, D.melanogaster: CENH3CID) is sufficient for centromere formation. It confers a stable and epigenetically heritable mark at the centromeric region and does this also on plasmids. With the help of CENH3 I changed the passive piggyback segregation mechanism of Epstein-Barr virus-derived vectors into an active segregation mechanism for plasmid DNA (pDNA) vectors. I demonstrated that the new and active pDNA vector segregation mechanism prolongs pDNA vector retention in cells. The information gained from basic research might have great impact in the field of gene-therapeutic research, as this mechanism might be a helpful tool in future gene therapeutic vectors.

Background: A key issue for safe and reproducible gene therapy approaches is the autologous and tissue-specific expression of transgenes. Tissue-specific expression in vivo is either achieved by transfer vectors that deliver the gene of interest into a distinct cell type or by use of tissue-specific expression cassettes. Here we present the generation of non-viral, episomally replicating vectors that are able to replicate in a tissue specific manner thus allowing tissue specific transgene expression in combination with episomal replication. The episomal replication of the prototype vector pEPI-1 and its derivatives depends exclusively on a transcription unit starting from a constitutively active promoter extending into the scaffold/matrix attachment region (S/MAR). Results: Here, we exchanged the constitutive promoter in the pEPI derivative pEPito by the tumor specific alpha fetoprotein (AFP) or the muscle specific smooth muscle 22 (SM22) promoter leading to specific transgene expression in AFP positive human hepatocellular carcinoma (HUH7) and in a SM22 positive cell line, respectively. The incorporation of the hCMV enhancer element into the expression cassette further boosted the expression levels with both promoters. Tissue specific-replication could be exemplary proven for the smooth muscle protein 22 (SM22) promoter in vitro. With the AFP promoter-driven pEPito vector hepatocellular carcinoma-specific expression could be achieved in vivo after systemic vector application together with polyethylenimine as transfection enhancer. Conclusions: In this study we present an episomal plasmid system designed for tissue specific transgene expression and replication. The human AFP-promoter in combination with the hCMV enhancer element was demonstrated to be a valuable tissue-specific promoter for targeting hepatocellular carcinomas with non-viral gene delivery system, and tissue specific replication could be shown in vitro with the muscle specific SM22 promoter. In combination with appropriate delivery systems, the tissue specific pEPito vector system will allow higher tissue-specificity with less undesired side effects and is suitable for long term transgene expression in vivo within gene therapeutical approaches.

Dr Arturo Reyes-Sandoval tells us about his research on a vaccine against Plasmodium vivax. Plasmodium vivax, one of the four malaria parasites that affect humans, is difficult to eradicate due to its ability to lay dormant in the liver for long periods of time. This parasite is found in Africa, Asia, Latin America and the Western Pacific. 40 percent of the world's population is exposed to the disease that is responsible for around 130 to 350 million clinical cases every year. Dr Arturo Reyes-Sandoval aims to develop a novel malaria vaccine against Plasmodium vivax.

Dr Arturo Reyes-Sandoval tells us about his research on a vaccine against Plasmodium vivax. Plasmodium vivax, one of the four malaria parasites that affect humans, is difficult to eradicate due to its ability to lay dormant in the liver for long periods of time. This parasite is found in Africa, Asia, Latin America and the Western Pacific. 40 percent of the world's population is exposed to the disease that is responsible for around 130 to 350 million clinical cases every year. Dr Arturo Reyes-Sandoval aims to develop a novel malaria vaccine against Plasmodium vivax.

Dr Lucy Dorrell tells us how our immune system controls HIV and how we can live with this virus. There are currently around 91,000 people in the UK living with HIV/AIDS. HIV is a challenging target for a vaccine because it can mutate its genetic makeup. Dr Lucy Dorrell aims to develop immunotherapy to reduce dependence on antiretroviral therapy (ART), the current standard treatment for those infected with HIV-1. Worldwide, 9 million of the estimated 33 million people living with HIV/AIDS today are not able to access the ARTs which they are in immediate need of.

Dr Lucy Dorrell tells us how our immune system controls HIV and how we can live with this virus. The aim of Dr Lucy Dorrells' research is to develop immunotherapy to reduce the dependence of those infected with HIV-1 on their current treatment - antiretroviral therapy (ART). This is because 9 million of the estimated 33 million people living with HIV/AIDS today are not able to access the ARTs which they are in immediate need of.

Dr Lucy Dorrell tells us how our immune system controls HIV and how we can live with this virus. There are currently around 91,000 people in the UK living with HIV/AIDS. HIV is a challenging target for a vaccine because it can mutate its genetic makeup. Dr Lucy Dorrell aims to develop immunotherapy to reduce dependence on antiretroviral therapy (ART), the current standard treatment for those infected with HIV-1. Worldwide, 9 million of the estimated 33 million people living with HIV/AIDS today are not able to access the ARTs which they are in immediate need of.

Dr Lucy Dorrell tells us how our immune system controls HIV and how we can live with this virus. The aim of Dr Lucy Dorrells' research is to develop immunotherapy to reduce the dependence of those infected with HIV-1 on their current treatment - antiretroviral therapy (ART). This is because 9 million of the estimated 33 million people living with HIV/AIDS today are not able to access the ARTs which they are in immediate need of.