Podcasts about hacs

Cromosomas Humanos ArtificialesEn este episodio, apto para todos los públicos, vamos a aprender sobre los cromosomas humanos artificiales (HACs), ¡una verdadera revolución en la biotecnología y la medicina!Descubre de manera sencilla cómo los HACs pueden transformar la terapia génica, la producción de medicamentos y la investigación genética. Te contaremos sobre las aplicaciones actuales y futuras de esta increíble tecnología, y cómo puede cambiar el tratamiento de enfermedades genéticas y el cáncer. Además, exploraremos el impacto de CRISPR en la creación e integración de HACs y discutiremos juntos los desafíos éticos y técnicos que enfrentamos.Un fascinante viaje hacia el futuro de la genética. ¡Y hasta tenemos trabalenguas! Un desafío que nos ayude a aprender y entender la revolución de los cromosomas artificiales.Capítulos: 00:00 Episodio 154403:27 Cromosoma07:57 Universidad de Pensilvania14:46 Multimerización26:25 HACs34:54 J Craig Venter38:34 CRISPR53:05 Medicamentos57:27 TrabalenguasBibliografía:Penn Medicine: Técnica HACsScience: Cromosomas artificialesNature: Artículo sobre CRISPRMedicina y Salud Pública: Cromosoma artificialPhys.org: Técnica HACsSciTechDaily: Implicaciones de HACsForo Económico: Genoma humanoMIT Tech: CRISPR y anemia falciformeBerkeley: Futuro de CRISPRIGI: Ensayos clínicos CRISPRMUNDIARIO: Inmunidad al cáncerEl País: Células inmunes al cáncerAntena 3: Cromosoma humano artificial#cromosomas humanos artificiales, HACs, terapia génica, producción de medicamentos, investigación genética, CRISPR, edición genética, enfermedades genéticas, cáncer, biotecnología, medicina personalizada, medicina, tecnología genética.Conviértete en un seguidor de este podcast: https://www.spreaker.com/podcast/el-siglo-21-es-hoy--880846/support.

Cromosomas Humanos ArtificialesEn este episodio, apto para todos los públicos, vamos a aprender sobre los cromosomas humanos artificiales (HACs), ¡una verdadera revolución en la biotecnología y la medicina!Descubre de manera sencilla cómo los HACs pueden transformar la terapia génica, la producción de medicamentos y la investigación genética. Te contaremos sobre las aplicaciones actuales y futuras de esta increíble tecnología, y cómo puede cambiar el tratamiento de enfermedades genéticas y el cáncer. Además, exploraremos el impacto de CRISPR en la creación e integración de HACs y discutiremos juntos los desafíos éticos y técnicos que enfrentamos.Un fascinante viaje hacia el futuro de la genética. ¡Y hasta tenemos trabalenguas! Un desafío que nos ayude a aprender y entender la revolución de los cromosomas artificiales.Capítulos: 00:00 Episodio 154403:27 Cromosoma07:57 Universidad de Pensilvania14:46 Multimerización26:25 HACs34:54 J Craig Venter38:34 CRISPR53:05 Medicamentos57:27 TrabalenguasBibliografía:Penn Medicine: Técnica HACsScience: Cromosomas artificialesNature: Artículo sobre CRISPRMedicina y Salud Pública: Cromosoma artificialPhys.org: Técnica HACsSciTechDaily: Implicaciones de HACsForo Económico: Genoma humanoMIT Tech: CRISPR y anemia falciformeBerkeley: Futuro de CRISPRIGI: Ensayos clínicos CRISPRMUNDIARIO: Inmunidad al cáncerEl País: Células inmunes al cáncerAntena 3: Cromosoma humano artificial#cromosomas humanos artificiales, HACs, terapia génica, producción de medicamentos, investigación genética, CRISPR, edición genética, enfermedades genéticas, cáncer, biotecnología, medicina personalizada, medicina, tecnología genética.Conviértete en un seguidor de este podcast: https://www.spreaker.com/podcast/el-siglo-21-es-hoy--880846/support.

Cromosomas Humanos ArtificialesEn este episodio, apto para todos los públicos, vamos a aprender sobre los cromosomas humanos artificiales (HACs), ¡una verdadera revolución en la biotecnología y la medicina!Descubre de manera sencilla cómo los HACs pueden transformar la terapia génica, la producción de medicamentos y la investigación genética. Te contaremos sobre las aplicaciones actuales y futuras de esta increíble tecnología, y cómo puede cambiar el tratamiento de enfermedades genéticas y el cáncer. Además, exploraremos el impacto de CRISPR en la creación e integración de HACs y discutiremos juntos los desafíos éticos y técnicos que enfrentamos.Un fascinante viaje hacia el futuro de la genética. ¡Y hasta tenemos trabalenguas! Un desafío que nos ayude a aprender y entender la revolución de los cromosomas artificiales.Capítulos: 00:00 Episodio 154403:27 Cromosoma07:57 Universidad de Pensilvania14:46 Multimerización26:25 HACs34:54 J Craig Venter38:34 CRISPR53:05 Medicamentos57:27 TrabalenguasBibliografía:Penn Medicine: Técnica HACsScience: Cromosomas artificialesNature: Artículo sobre CRISPRMedicina y Salud Pública: Cromosoma artificialPhys.org: Técnica HACsSciTechDaily: Implicaciones de HACsForo Económico: Genoma humanoMIT Tech: CRISPR y anemia falciformeBerkeley: Futuro de CRISPRIGI: Ensayos clínicos CRISPRMUNDIARIO: Inmunidad al cáncerEl País: Células inmunes al cáncerAntena 3: Cromosoma humano artificial#cromosomas humanos artificiales, HACs, terapia génica, producción de medicamentos, investigación genética, CRISPR, edición genética, enfermedades genéticas, cáncer, biotecnología, medicina personalizada, medicina, tecnología genética.Conviértete en un seguidor de este podcast: https://www.spreaker.com/podcast/el-siglo-21-es-hoy--880846/support.

Polo Ramírez estuvo en Ruta Silvestre junto a José Francisco Muñoz, gerente general Termas de Chillán, comentando lo que se viene para el hotel en esta nueva temporada. Además, conversó con Carolina Gainza Cortés, la subsecretaria de Ciencia, Tecnología, Conocimiento e Información que se refirió al primer estudio que sistematiza evidencia sobre Humanidades, Artes y Ciencias Sociales en Chile (HACS).

Lots of SINs descriptions have been updated, including some of the major IT SINs like HACS and Cloud.  Hop into the episode to catch up on the essential details and see how it will effect your GSA ambitions.As always if you have any questions, or if you'd like direct support from a GSA consultant to help with a specific project please feel free to reach out to us at podcast@elevategsa.com Episode Notes:For more episodes: www.elevategsa.com/podcastHelpful Links:GSA Interact Refresh 20: https://buy.gsa.gov/interact/community/6/activity-feed/post/c67499eb-ba3d-4382-980f-73f4808386b2/Advanced_Notice_for_MAS_Refresh_20_and_Upcoming_Mass_Modification?utm_medium=email&utm_source=govDeliveryGSA Interact Refresh 21: https://buy.gsa.gov/interact/community/6/activity-feed/post/ddc2720c-c9cb-4a76-9a44-98a056428ebf/Advanced_Notice_for_MAS_Refresh_21_and_Upcoming_Mass_Modification?utm_medium=email&utm_source=govDeliveryMass Mod Portal: https://mcm.fas.gsa.gov/cmservlet/massMods/initialView.htm

Today's guest is Kathy Dorich, MSN, RN, CCDS, CPHQ, director of Illinois CDI operations for Advocate Health and the recipient of the 2024 CDI Professional of the Year ACDIS Achievement Award, which was presented in a special ceremony at the 2024 ACDIS national conference in Indianapolis. Today's show is part of the “Conversations with Karla” series. In every episode of this series, ACDIS Editor and Product Coordinator Karla Kozak is joined by a guest who's behind one of our most popular and exciting ACDIS educational offerings to share their expertise. Our intro and outro music for the ACDIS Podcast is “medianoche” by Dee Yan-Kay and our ad music is “Take Me Higher” by Jahzzar, both obtained from the Free Music Archive. Have questions about today's show or ideas for a future episode? Contact the ACDIS team at info@acdis.org. Want to submit a question for a future "listener questions" episode? Fill out this brief form!  CEU info: Each ACDIS Podcast episode now offers 0.5 ACDIS CEUs which can be used toward recertifying your CCDS or CCDS-O credential for those who listen to the show in the first two days from the time of publication. To receive your 0.5 CEUs, go to the show page on acdis.org, by clicking on the “ACDIS Podcast” link located under the “Free Resources” tab. To take the evaluation, click the most recent episode from the list on the podcast homepage, view the podcast recording at the bottom of that show page, and click the live link at the very end after the music has ended. Your certificate will be automatically emailed to you upon submitting the brief evaluation. (Note: If you are listening via a podcast app, click this link to go directly to the show page on acdis.org: https://acdis.org/acdis-podcast/quality-deep-dive-psis-and-hacs) Note: To ensure your certificate reaches you and does not get trapped in your organization's spam filters, please use a personal email address when completing the CEU evaluation form. The cut-off for today's episode CEU is Monday, April 15, at 11:00 p.m. eastern. After that point, the CEU period will close, and you will not be eligible for the 0.5 CEUs for this week's episode. Because of the ACDIS conference this week and associated travel, the ACDIS team has extended the deadline for this episode. Today's sponsor: Today's show is brought to you by the 2024 ACDIS Outpatient Pocket Guide. Learn more and order your copy by clicking here. (https://bit.ly/43GQ441) ACDIS update: The ACDIS office will be closed until Monday, April 15, due to the ACDIS conference and associated travel. Feel free to email the team at info@acdis.org, but please note that responses may be delayed this week! Read about the 2024 ACDIS Achievement Award winners and offer your congratulations! (https://bit.ly/4cHoCqQ)

Medicare's Hospital-Acquired Conditions Reduction Program (HACRP) is a value-based-purchasing program for Medicare that aims to link Medicare payments to a hospital's level of inpatient health care quality. Jacob Madden, Research Specialist, Program on Regulation, Therapeutics, and Law, Harvard Medical School and Brigham and Women's Hospital, and Christina Yen, MD, MBE, Medical Director of Antimicrobial Stewardship, Maine Medical Center, discuss how the HACRP works, how hospital-acquired conditions (HACs) affect hospitals' ability to care for patients, how a hospital's HAC score is calculated, what happened to the HACRP during the pandemic, and what a successful post-pandemic HACRP might look like. Jacob recently authored an article for AHLA's Journal of Health and Life Sciences Law about this issue.To learn more about AHLA and the educational resources available to the health law community, visit americanhealthlaw.org.

In this episode of Mind Body Peak Performance, host Nick Urban talks with Regan Archibald, founder of East West Health Clinic and a distinguished authority in the realm of peptides. You'll learn about stem cell rejuvenation, ancient Eastern therapies, and cutting-edge treatments that optimize health across different life stages. Explore diagnostic tools essential for precise health management.Meet our guestRegan Archibald, Lac, FMP, a renowned Peptide Specialist and founder of East West Health and Integrated Pain Specialists. With a trailblazing approach to health, Regan merges Acupuncture, Regenerative Medicine, and Functional Medicine through his Medically Managed Peptide Program at East West Health. As the brains behind the "Health Accelerator Course," he engages over 1000 members weekly in transformative "HACs" and hosts the insightful podcast, Never Stop Healing. Beyond his expertise, you'll find Regan conquering Wasatch mountains, indulging in ice baths, and infusing artistry into medicine, all while cherishing moments with his wife Jessica, and three kids.Thank you to our partners Outliyr Peak Performance Shop: get exclusive discounts on cutting-edge health, wellness, & performance gear Unlock your built-in regenerative capabilities with the latest Brown's Gas technology (read my review and use code URBAN to save 5%) Level up your gut health with the most powerful, 28-ingredient, all-on-one Qualia Synbiotic formula (code URBAN saves 15%)Key takeaways How peptides optimize our health through different stages of life Health benefits of stem-cell rejuvenation How Eastern therapies, such as acupuncture and herbs complement Western protocols like peptides & stem cell therapies Efficacy of Eastern therapies, acupuncture & herbal medicine Importance of using intuition as a lens to manage diagnoses and treatment optionsLinks Watch it on YouTube: https://youtu.be/Vs6vR8Z7-c4 Full episode show notes: https://mindbodypeak.com/118Connect with Nick on social media Instagram Twitter YouTube LinkedInEasy ways to support Subscribe Leave an Apple Podcast review Suggest a guestRelated shows Ep 82: Your Custom Protocol To Reverse Your “Bioage” & Stay Young EP 113: Harnessing Therapeutic Testosterone & Peptides to Optimize Mind, Body & Spirit (P1)Do you have questions, thoughts, or feedback for us? Let me know in the show notes above and one of us will get back to you!Be an Outliyr,Nick

Avenue 360 "Pets in the Park" -- Book of Mary -- Stages Theater " The Bride"We speak with Dr. Charlene Flash, President CEO of Avenue 360 talks about the organization and the upcoming Pets in the Park event. Avenue 360 Health and Wellness, a 501 (c)3 organization, is a Federally Qualified Health Center with seven clinics in the Houston region providing primary, behavioral health, and dental care to medically underserved communities. Previously, the organization was known as Houston Area Community Services (HACS), founded in 1998 to treat those living with AIDS. Several years ago, HACS partnered with Bering Omega (Omega House), which provided hospice care to people living with HIV/AIDS, to form Avenue 360. The mission of Avenue 360 is to provide high quality and caring services to promote healthy people and communities.   Pet Pics in the Park, hosted by Chris Christopher Properties. With Honorary Chair Ed Holmstrom, this special event will be held on March 25th from 10 am – 2 pm, and photos with your pet will be taken by Houston's #1 Pet Photographer, Jill Garrett. Proceeds from the event will go towards Omega House hospice services, where compassionate hospice care is provided to men and women with HIV/AIDS.  Guest: Dr. Charlene Flash/Christopher Cerdahttps://petpicsinthepark.orgThen, we speak with Mary Hooper about her autobiographical show, "Book of Mary". The Book of Mary (It Costs a Lot to Be Real), is an autobiographical one-woman show, written by and starring Houston's own Mary Hooper, and directed by Ted Swindley, founder of Houston's STAGES theatre and author of the popular musical, Always…Patsy Cline. It's a raucous ride: from her Beaumont beginnings as a shy child of deaf parents, her timely escape to the gay neighborhood of Montrose in the 70s and 80s, to her adventures onstage in Houston's way off Broadway theatre scene. Both funny and poignant, The Book of Mary is a love letter to the diva in us all.Guest: Mary Hooperhttps://matchouston.org/events/2023/book-maryFinally, we speak with Denise Fennel about "The Bride" currently in production at Stages Theater. Beloved fan-favorite known for her role as Sister in Late Nite Catechism, Denise Fennell returns to Stages for an all-new, hysterical comedy showcase. With less than two hours until her wedding, a bride of a certain age begins to question the meaning of love, life, and the ritual of marriage. Drawing inspiration from her own life and the advice of the audience, The Bride will lead to the decision of a lifetime: Will she or won't she?Guest: Denise Fennelhttps://stageshouston.com/event/denise-fennells-the-bride-or-does-this-dress-make-me-look-married/

Hace años monté Home Assistant y no me convenció, me resultó muy farragoso y complejo de configurar, pero a día de hoy las cosas han cambiado mucho y son bastante más sencillas. Os cuento cómo lo he montado, lo que más me está gustando y por qué se va a quedar conmigo.

Pomegranate Health is seeking fresh ears on the Podcast Editorial Group. Responsibilities of group members are to> discuss new podcast topics> suggest themes to explore and people to interview> listen to audio drafts and provide feedback before publicationGroup communication is done informally by email and there's a time commitment of approximately 90 minutes per month. Please download an application form and return it before February 2023 to podcast@racp.edu.au. ******Clinical complications suffered by patients during hospital stays are assumed to be preventable and to provide some metric of quality of care. To assist in their understanding and mitigation the Australian Commission on Safety and Quality in Healthcare established a national programme to track hospital-acquired complications (HACs) in a formalised way. Comparison data can be found through the Health Roundtable reports and it's been understood that hospitals with higher complication rates may have a have a lower standard of care. While the national HAC program has support from providers across all jurisdictions and makes good use of electronic medical records, some questions remain as to its methodology. In a retrospective audit of medical records published in the Internal Medicine Journal, Dr Graeme Duke and colleagues at Eastern Health Intensive Care Research have sought to validate the clinical significance of HACs identified within their service. Their research suggests that HACs are underreported by coding data and that they are more strongly associated with patient-related factors than with deviation from clinical best practice. Dr Graeme and IMJ editor Professor Ian Scott discuss the research article and its implications for the national hospital-acquired complications programme.  GuestsDr Graeme Duke FCICM, FANZCA (Eastern Health Intensive Care Services)Prof Ian Scott FRACP (University of Queensland, Princess Alexandra Hospital)ReferencesGraeme J Duke et al. Clinical evaluation of the national hospital-acquired complication programme Internal Medicine Journal 2021; 52(11); 1910-1916 Access to IMJ, JPCH and OMJ for RACP membersProductionWritten and produced by Mic Cavazzini DPhil. Music licenced from Epidemic Sound includes ‘Reaching for Infinity' by Dawn Dawn Dawn and ‘Nabga Algooah' by Ebo Krdum. Image by SolStock licenced from Getty Images.Please visit the Pomegranate Health web page for a transcript and supporting references. To claim learning credits login to MyCPD at this link, review the prefilled activity details and click save. Subscribe to new episode email alerts or search for ‘Pomegranate Health' in Apple Podcasts, Spotify, Castbox, or 

Rohan and Phil break down the 2022.8 release, Phil discusses a new custom component he's found in HACS for energy dashboard users For complete show notes and more information about the topics discussed in this episode, be sure to check the notes at https://hasspodcast.io/ha113/ This episode was made possible thanks to our sponsor Home Assistant Cloud by Nabu Casa Easily connect to Google and Amazon voice assistants for a small monthly fee that also supports the Home Assistant project. Configuration is via the User Interface so no fiddling with router settings, dynamic DNS or YAML. Website: https://nabucasa.com ----- Hosts ----- Phil Hawthorne Website: https://philhawthorne.com Smart Home Products: https://kit.co/philhawthorne Twitter: https://twitter.com/philhawthorne Buy Phil a Coffee: https://buymeacoff.ee/philhawthorne Rohan Karamandi Website: https://karamandi.com Smart Home Products: https://kit.co/rkaramandi/ Twitter: https://twitter.com/rohank9 Buy Rohan a Coffee: https://buymeacoff.ee/rkaramandi

Getting to the clinical truth is the real purpose of coding, according to our two experts on this episode of our award-winning podcast. We'll continue our discussion on avoiding defensive coding practices and improving clinical data and reimbursement.   Guest speakers: Jim Tamburini, BS, RHIT, CCS, CCS-P, CDIP Senior Consulting Director Vizient   Sheila Bowlds, MBA Senior Consulting Director Vizient   Moderator: Tomas Villanueva, DO, MBA, FACPE, SFHM Principal Clinical Operations and Quality Vizient   Show Notes: [01:15] Fear of denials: the basis of conservative coding practices [01:55] Proactive coding [02:32] Getting to the true clinical story [04:11] Physician involvement in getting to the truth [06:22] Dealing with patient safety indicators (PSIs) and hospital-acquired conditions (HACs) [08:47] Vizient's support [10:38] Clinical language vs. coding language [12:08] Issues around copying and pasting   Links | Resources: To contact Modern Practice: modernpracticepodcast@vizientinc.com Jim Tamburini's emaill: james.tamburini@vizientinc.com Sheila Bowlds' email: sheila.bowlds@vizientinc.com Are you prepared to defend your coding? (AAFP) Click Here   Subscribe Today! Apple Podcasts Amazon Podcasts Android Google Podcasts Spotify Stitcher RSS Feed

The gang brings on real fleet commanders to discuss the changes that fleet formations will bring to the game. We also talk about updates to Marauders, heavy assault cruisers, and more. --- Support this podcast: https://anchor.fm/trashtalktuesday/support

In this final episode of our series on HACs, we examine the challenge of Clostridium difficile infection (C. diff, or CDI) for clinicians and patients. Highly infectious and unsusceptible to alcohol, CDI is cause for concern, with infection rates rising after COVID-19, as they are for all HACs. Yet, a systematic approach according to new guidelines can curb and eliminate this condition.   Guest speaker: Richard Beaver, B.S. Chem, MBB, CPHQ Senior Consulting Director Vizient   Moderator: Tomas Villanueva, DO, MBA, FACPE, SFHM Principal Clinical Operations and Quality Vizient   Show Notes: [00:31] New CDI guidelines [01:21] Insight on major causes – and an example [03:31] Benefit of early detection at hospital [05:57] Issues with testing protocols, isolation practices and use of antibiotics [09:21] A multi-faceted approach   Links | Resources: Rick Beaver's contact email: richard.beaver@vizientinc.com New CDI guidelines (IDSA and SHEA) Click here CMS Hospital-Acquired Condition (HAC) fact sheet Click here CMS Hospital-Acquired Condition (HAC) reduction program Click here Information on Vizient's Clinical Data Base (CDB) Click here AHRQ tools to reduce HACs Click here   Subscribe Today! Apple Podcasts Spotify Android Google Podcasts Stitcher RSS Feed

Surgical site infections (SSIs) are persistent and challenging, no more so than following the COVID-19 outbreak. Now that elective surgeries are ramping up again, it is more important than ever to prevent SSIs, and that is the focus for this episode in our series addressing HACs.   Guest speaker: Richard Beaver, B.S. Chem, MBB, CPHQ Senior Consulting Director Vizient   Moderator: Tomas Villanueva, DO, MBA, FACPE, SFHM Principal Clinical Operations and Quality Vizient   Show Notes: [00:36] The upsurge in sepsis [02:25] Limited SSI improvement in some cases [03:39] A combination of causes [04:50] Lack of compliance [05:59] Prophylactic antibiotics [08:35] Chlorhexidine [10:33] Accurate metrics and analysis for key drivers of improvement [11:35] 360-degree approach   Links | Resources: Rick Beaver's contact email: richard.beaver@vizientinc.com CMS Hospital-Acquired Condition (HAC) fact sheet Click here CMS Hospital-Acquired Condition (HAC) reduction program Click here Information on Vizient's Clinical Data Base (CDB) Click here AHRQ tools to reduce HACs Click here   Subscribe Today! Apple Podcasts Spotify Android Google Podcasts Stitcher RSS Feed  

The outbreak of COVID-19 exacerbated the occurrence of Hospital-Acquired Conditions (HACs) in health care organizations, and bringing infection rates back down is a struggle. This series examines the steps we can take to reduce HACs, and on this episode, we focus on catheter-associated urinary tract infections, or CAUTIs.   Guest speaker: Richard Beaver, B.S. Chem, MBB, CPHQ Senior Consulting Director Vizient   Moderator: Tomas Villanueva, DO, MBA, FACPE, SFHM Principal Clinical Operations and Quality Vizient   Show Notes: [00:33] Occurrences of CAUTI [01:11] Foley removal practice [05:51] Other causes and managing the perineum [08:25] Addressing the bundle components   Links | Resources: Rick Beaver's contact email: richard.beaver@vizientinc.com CMS Hospital-Acquired Condition (HAC) fact sheet Click here CMS Hospital-Acquired Condition (HAC) reduction program Click here Information on Vizient's Clinical Data Base (CDB) Click here AHRQ tools to reduce HACs Click here   Subscribe Today! Apple Podcasts Spotify Android Google Podcasts Stitcher RSS Feed  

Hospital-Acquired Conditions (HACs) have risen in frequency since the outbreak of COVID-19 after years when rates were falling. This series examines the reasons and steps we can take to reduce these infections. On this episode, we focus on central line-associated bloodstream infections, or CLABSIs.   Guest speaker: Richard Beaver, B.S. Chem, MBB, CPHQ Senior Consulting Director Vizient   Moderator: Tomas Villanueva, DO, MBA, FACPE, SFHM Principal Clinical Operations and Quality Vizient   Show Notes: [00:31] Effect of placement on line management [04:12] Impact of shaving [06:18] Actions for preventing CLABSIs [08:23] Engaging physicians and nurses – not dictating to them   Links | Resources: Rick Beaver's contact email: richard.beaver@vizientinc.com CMS Hospital-Acquired Condition (HAC) fact sheet Click here CMS Hospital-Acquired Condition (HAC) reduction program Click here Information on Vizient's Clinical Data Base (CDB) Click here AHRQ tools to reduce HACs Click here   Subscribe Today! Apple Podcasts Spotify Android Google Podcasts Stitcher RSS Feed  

Even before the pandemic, health care organizations struggled with Hospital-Acquired Conditions (HACs), and the outbreak of COVID-19 only worsened infection rates. In this five-part series, we will examine four leading HACs to discuss why their rates went up and proven approaches hospital staff can use to reduce them.   Guest speaker: Richard Beaver, B.S. Chem, MBB, CPHQ Senior Consulting Director Vizient   Moderator: Tomas Villanueva, DO, MBA, FACPE, SFHM Principal Clinical Operations and Quality Vizient   Show Notes: [01:05] Situation today regarding HACs, as seen in the Vizient Clinical Data Base (CDB) [01:45] Major HACs [02:21] Effect of the pandemic [03:17] Insights post-COVID [07:15] Line placement: who owns the lines [08:52] Impactful HACs [10:06] Role of clinician resilience [11:24] Financial implications/penalties   Links | Resources: Rick Beaver's contact email: richard.beaver@vizientinc.com CMS Hospital-Acquired Condition (HAC) fact sheet Click here CMS Hospital-Acquired Condition (HAC) reduction program Click here Information on Vizient's Clinical Data Base (CDB) Click here AHRQ tools to reduce HACs Click here   Subscribe Today! Apple Podcasts Spotify Android Google Podcasts Stitcher RSS Feed

『新 基礎情報学：機械をこえる生命』 西垣通｜NTT出版｜2021年6月11日 朗読箇所｜第3部「人間のための情報技術／AIという衝撃」「ホモデウスが到来するとき」より（P.161〜182） 反ホモ・デウスのために―トランス・ヒューマニズムとデータ至上主義の誤謬を証し、ディストピアを回避する方途を理論的に探る、〈基礎情報学〉の決定版。多様な人間/生命が息づく未来に向け西垣情報学理論の集大成。 【目次】 序論　反ホモ・デウスのために 　復活したトランス・ヒューマニズムの亡霊 　AIをめぐる難題と混乱 　二つのパラダイムそしてネオ・サイバネティクス 　基礎情報学から見た“人間=機械〝複合系〟 　本書の構成 第Ⅰ部　基礎情報学にいたるアプローチ/情報と意味創出 第1章　ネオ・サイバネティクスの誕生 　1・1　情報をめぐる二つのパラダイム 　1・2　論理主義とコンピューティング・パラダイム 　1・3　コンピュータと情報理論 　1・4　サイバネティック・パラダイムの端緒 　1・5　多元宇宙の哲学 　1・6　二次サイバネティクスと構成主義 第2章　ネオ・サイバネティクスの展開 　2・1　オートポイエーシス理論 　2・2　機能的分化社会理論 　2・3　ラディカル構成主義心理学 　2・4　システム文学論 第Ⅱ部　基礎情報学の核心/生命にもとづく情報学 第3章　APSからHACSへ 　3・1　情報を定義する 　3・2　生命情報/社会情報/機械情報 　3・3　HACS 　3・4　情報/コミュニケーション/メディア 　3・5　プロパゲーション 　3・6　個人と社会の知識形成 　3・7　新たな発展へ 第4章　新実在論と生命哲学 　4・1　情報学的転回 　4・2　新実在論の台頭 　4・3　生命論からの考察 　4・4　内部観測 第Ⅲ部　人間のための情報技術/AIという衝撃 第5章　AIの論理と誘惑 　5・1　AIのもたらす「偽―情報学的転回」 　5・2　トランス・ヒューマニストたちの主張 　5・3　ホモ・デウスが到来するとき 　5・4　情報圏という罠 　5・5　この国のAIアプローチ 第6章　データ至上主義からの脱出 　6・1　情報学と物質科学の違い 　6・2　ホモ・デウスを拒む 　6・3　二つのパラダイムの接点 　6・4　AI時代の癒し 　6・5　AIのコントロール 　6・6　AI時代の自律性 　6・7　次世代の情報教育 https://www.nttpub.co.jp/search/books/detail/100002518.html 企画・朗読：若林恵 録音・編集：山口宜大（Magic Mill Sounds） 音楽：yasuhiro morinaga + maiko ishii 黒鳥福祉センターにて収録

Costruire un sistema domotico in pochi e semplici passi, senza impazzire con le configurazioni, usando Docker, ma senza saperlo usare. Insomma, un sogno che si avvera. Se avete tutti i componenti (Un Raspberry Pi, Una microSD seria, e un alimentatore), il sistema si tira su in una serata e funziona tutto al primo colpo! Introduzione a DockerIl sito di IOTStackDove scaricare l'immagine di Raspberry OSLa guida per iniziareL'installazione va fatta con il cavo ETH collegato e non con la WiFi, perché la procedura vi sconfigura la rete almeno 3 volteDal menu installate Portainer-ce e Portainer-agent (si accede via web da http://ip_raspberry:9000)Installare da riga di comando due pacchetti indispensabili (non lo abbiamo detto nel podcast, ma è importante): sudo apt-get install network-manager apparmor-utilsPer fare l'installazione più completa: Native Install e poi Hass.io SupervisorPer installare HACS: wget -q -O - https://hacs.xyz/install | bash - (dalla console del suo container)Backup e restore delle SD dei raspberryLe valvole termostatiche NetAtmoOptimus Prime e i suoi 27 servomotori Pillole di Geek è un podcast di Francesco e Giuliano, per entrare in contatto con la community puoi iscriverti e partecipare al forum. L'hosting è gentilmente offerto da ThirdEye, per avere un'offerta dei loro servizi scrivi a domini[at]thirdeye.it Se vuoi contribuire economicamente al podcast (cosi possiamo cedere alle tentazioni), puoi donare con Satispay o con Paypal, ci affidiamo al tuo buon cuore, grazie! La sigla è "Soundside" dell'album "Hi Tech Trailer"

Dula Mac - HACS [BayAreaCompass] by BayArea Compass

Esta semana El Tren Sin Pasajeros hace homenaje a Héctor Cervantes, Alias el HACS, les invito a escucharlo. https://www.youtube.com/channel/UC2C3j4K8j06NWBiIVZHPkuQ Está historia y si música son 9bra única y hermosa de Alis el HACS. Escúchenla y déjenme su bonito comentario. Buenas noches --- Send in a voice message: https://anchor.fm/oscarglez/message

Esta semana El Tren Sin Pasajeros hace homenaje a Héctor Cervantes, Alias el HACS, les invito a escucharlo. https://www.youtube.com/channel/UC2C3j4K8j06NWBiIVZHPkuQ --- Send in a voice message: https://anchor.fm/oscarglez/message

A manera de réplica y cerrando este ciclo en que he querido hacer un homenaje al talento de mis grandes amigos, les comparto este pequeño texto agradeciendo como siempre su compañía en este Tren Sin Pasajeros. Yo soy el Halcón nocturno. Buenas noches. Héctor Cervantes es Alias el HACS, les invito a escucharlo. https://www.youtube.com/channel/UC2C3j4K8j06NWBiIVZHPkuQ --- Send in a voice message: https://anchor.fm/oscarglez/message

Esta semana El Tren Sin Pasajeros hace homenaje a Héctor Cervantes, Alias el HACS, les invito a escucharlo. https://www.youtube.com/channel/UC2C3j4K8j06NWBiIVZHPkuQ --- Send in a voice message: https://anchor.fm/oscarglez/message

Esta semana El Tren Sin Pasajeros hace homenaje a Héctor Cervantes, Alias el HACS, les invito a escucharlo. https://www.youtube.com/channel/UC2C3j4K8j06NWBiIVZHPkuQ --- Send in a voice message: https://anchor.fm/oscarglez/message

Esta semana El Tren Sin Pasajeros hace homenaje a Héctor Cervantes, Alias el HACS, les invito a escucharlo. https://www.youtube.com/channel/UC2C3j4K8j06NWBiIVZHPkuQ --- Send in a voice message: https://anchor.fm/oscarglez/message

Esta semana El Tren Sin Pasajeros hace homenaje a Héctor Cervantes, Alias el HACS, les invito a escucharlo. https://www.youtube.com/channel/UC2C3j4K8j06NWBiIVZHPkuQ --- Send in a voice message: https://anchor.fm/oscarglez/message

This week I'm doing what normally gets a lot of listens on The Lone Wolf Podcast, Having a Conversation. The show where I talk to people for no reason whatsoever. For this week, I'm with Pink Penguin this week and all we did just talked about Yu-Gi-Oh and episode 38 of Yu-Gi-Oh Sevens while we were at. Hopefully I able to have more HACs with this podcast in the future. *IMPORTANT I am a human being with opinions, therefore my opinion is biased to an extent. PLEASE for my sanity's sake DO NOT go to my social media or email and say that I'm wrong and here's why. It's okay to disagree with me, as long you give evidence to support your claim (via social media) Please respect my opinion and I will respect yours. (Even though this is a very small podcast, I still have an audience and wanted to say this earlier than later because people are scary and I don't know when they will attack me) SOCIALS Twitter: https://twitter.com/LoneWolfNerd Instagram: https://www.instagram.com/the_lone_nerd_26 Questions, Comments or Concerns: wolfthelone90@gmail.com Listen to The Lone Wolf on Apple Podcasts: https://podcasts.apple.com/podcast/id1508766768 Listen to The Lone Wolf on Spotify: https://open.spotify.com/show/3oO38FBABdFEwMToY7v4Tx Listen to The Lone Wolf Dormitory on Spotify: https://open.spotify.com/show/1a5k44dH9TWIjnAvu4rDYn Listen to The Lone Wolf Dormitory on Apple Podcasts: https://podcasts.apple.com/us/podcast/the-lone-wolf-dormitory/id1536830665 Follow P Egoist YouTube: https://www.youtube.com/c/PEgoist/featured Follow Pink Penguin on Youtube: https://www.youtube.com/channel/UCk69JuvgSh9y3cvrkLxjFLw/featured Follow Pink Penguin on Twitch: https://www.twitch.tv/shiningbutterfly4 --- This episode is sponsored by · Anchor: The easiest way to make a podcast. https://anchor.fm/app

HACs, Interdictors, and Marauders. 

Successful open-source projects all seem to struggle with one major gorilla. Who it is, and what their options are now. Special Guests: Drew DeVore and Jonathan Corbet.

In this episode of Author to Author, Dr. Cynthia Toolin-Wilson interviews Dr. Arthur William McVey on his book Soulful Organizational Leadership (January 6, 2021)In the “Postmodern” organizational era, the concept of soul, spirituality, rationality, telos, virtue, happiness, and meaning is becoming increasingly a mainstream component in scientific psychology. This situation presents a promising challenge for a Thomistic, organizational faculty-behavioral-psychology. As contemporary organizations emerge into hierarchical-heterarchical structures, it is argued that a return to a premodern Aristotelian-Thomistic, faculty-behavioral-psychology is the most appropriate organizational psychology for the digital-disruptive culture in quest of an executive-exemplar realist leadership. In responding to this cultural situation, we will develop a psychology of soulful organizational leadership grounded on a Thomistic, organizational faculty-behavioral-psychology.https://enroutebooksandmedia.com/leadership/

Mais um novo produto aqui pra você: “Um diário velho e surrado foi encontrado, nele O post Diário de Hacs – Capítulo 1 – Parte 4 apareceu primeiro em FLIPERAMA DE BOTECO.

Mais um novo produto aqui pra você: “Um diário velho e surrado foi encontrado, nele O post Diário de Hacs – Capítulo 1 – Parte 4 apareceu primeiro em FLIPERAMA DE BOTECO.

Mais um novo produto aqui pra você: “Um diário velho e surrado foi encontrado, nele O post Diário de Hacs – Capítulo 1 – Parte 3 apareceu primeiro em FLIPERAMA DE BOTECO.

Mais um novo produto aqui pra você: “Um diário velho e surrado foi encontrado, nele O post Diário de Hacs – Capítulo 1 – Parte 3 apareceu primeiro em FLIPERAMA DE BOTECO.

Mais um novo produto aqui pra você: “Um diário velho e surrado foi encontrado, nele O post Diário de Hacs – Capítulo 1 – Parte 2 apareceu primeiro em FLIPERAMA DE BOTECO.

Mais um novo produto aqui pra você: “Um diário velho e surrado foi encontrado, nele O post Diário de Hacs – Capítulo 1 – Parte 2 apareceu primeiro em FLIPERAMA DE BOTECO.

Mais um novo produto aqui pra você: “Um diário velho e surrado foi encontrado, nele O post Diário de Hacs – Capítulo 1 – Parte 1 apareceu primeiro em FLIPERAMA DE BOTECO.

Mais um novo produto aqui pra você: “Um diário velho e surrado foi encontrado, nele O post Diário de Hacs – Capítulo 1 – Parte 1 apareceu primeiro em FLIPERAMA DE BOTECO.

In this episode of The Two Wings Seminar, Dr. Daniel Van Slyke, former Academic Dean of Online Learning at Holy Apostles College & Seminary, interviews Fr. John Cush, current Academic Dean of the Pontifical North American College in Rome (December 29, 2019)

In this episode of The Two Wings Seminar, Dr. Daniel Van Slyke, former Academic Dean of Online Learning at Holy Apostles College & Seminary, interviews Fr. John Cush, current Academic Dean of the Pontifical North American College in Rome (December 29, 2019)

The General Services Administration has updated a cybersecurity services offering available to agencies using its IT Schedule 70 program. The highly adaptable cybersecurity services, or HACS, deal exists as what GSA calls a special item number, or SIN. For what's new and what it will do for cybersecurity, the director of the IT security subcategory, in the Office of IT Category Management Larry Hale joined Federal Drive with Tom Temin.

Avoidable Hospital Infections Guest Opportunity: Ty and Carole Moss - Preventative Hospital Solutions Crusaders and Founders of “Nile’s Project” A set of 14 hospital-acquired conditions that the CMS considers avoidable accounted for 48,771 adverse patient outcomes, 3,219 deaths and more than $2 billion in excess hospital costs in 2016, according to a new research brief. Patients experiencing these hospital-acquired conditions (HACs) were in the hospital about 8 days longer on average than other patients, with an average excess hospital cost of $41,917and their increased mortality risk was 72.3%. How can we eliminate unnecessary deaths from MRSA, VRE, Staph and other preventable hospital acquired infections. You and your family deserve clean, safe care. Ask for it. Here are some steps…… Ask that hospital staff clean their hands before treating you and ask visitors to clean their hands. Before your doctor uses a stethoscope ask that the diaphragm (the flat surface) be wiped with alcohol. If you need a “central line” catheter, ask your doctor about the benefits of one that is antibiotic-impregnated or antiseptic-coated to reduce infections. If you need surgery, choose a surgeon with a low infection rate. Ask your doctor about keeping you warm during surgery. These are just a few of the steps needed to stay clean. Ty and Carole Moss have been leaders in the awareness charge when it comes to hospital cleanliness and can elaborate on the rest of the steps for you or a loved one to stay clean! Ty and Carole Moss can use their experience with the unfortunate death of their 15 year old to elaborate on the need to act fast, early identification, appropriate rapid treatment and preventative solutions they have learned by their scientific and expert partners at the CDC (The CDC Centers For Disease Control and Prevention). The Moss’s include lifesaving information the CDC recently launched their national Get Ahead of Sepsis "know the signs act fast” campaign and offer their take on "Nile's Project" as it gives back to the community. For the last several years the Moss family has been engaged in making healthcare safer from a local, state and federal level. They have identified many critical areas of healthcare, policy, prevention, and awareness that cost the life of their son. They are eager to share their findings along with solutions that can prove to be lifesaving. Meet Ty and Carole Moss: Ty Moss is an American Musician, Song Writer Music Producer wi

HESTA chief executive Debby Blakey on a new report that explores the movements of health and community services (HACS) workers.

HESTA chief executive Debby Blakey on a new report that explores the movements of health and community services (HACS) workers.

Following the lead of Pope Benedict XVI and synthesizing insights from his book on the subject, in this lecture Dr. Matthew Ramage will set out to answer these above questions by wedding the historical-critical approach with a theological reading of Scripture based in the patristic-medieval tradition. Whereas these two approaches are often viewed as mutually exclusive or even contradictory, Ramage will insist that the two are mutually enriching and necessary for doing justice to the Bible's most challenging texts. (Recorded and aired on Friday, February 2, 2018.)Dr. Matthew Ramage is Adjunct Professor of Theology at HACS and Associate Professor of Theology at Benedictine College. He is author, contributing author, or co-translator of several books, including the monographs Dark Passages of the Bible: Engaging Scripture with Benedict XVI and Thomas Aquinas and Jesus, Interpreted: Benedict XVI, Bart Ehrman, and the Historical Truth of the Gospels. Dr. Ramage's work has appeared in a number of scholarly journals including Nova et Vetera, Scripta Theologica, Cithara, and Homiletic and Pastoral Review as well as popular online venues such as Strange Notions, The Gregorian Institute, and Crisis. He has been interviewed by news outlets such as the National Catholic Register andFirst Things and has made periodic appearances on the EWTN programs Catholic Answers Live, Catholicism on Campus, and The Son Rise Morning Show. See https://www.catholic.com/audio/cal/8360#.WlmNNGt_vzAFor more on his work and his CV, visit Dr. Ramage's website www.truthincharity.com.

Following the lead of Pope Benedict XVI and synthesizing insights from his book on the subject, in this lecture Dr. Matthew Ramage will set out to answer these above questions by wedding the historical-critical approach with a theological reading of Scripture based in the patristic-medieval tradition. Whereas these two approaches are often viewed as mutually exclusive or even contradictory, Ramage will insist that the two are mutually enriching and necessary for doing justice to the Bible's most challenging texts. (Recorded and aired on Friday, February 2, 2018.)Dr. Matthew Ramage is Adjunct Professor of Theology at HACS and Associate Professor of Theology at Benedictine College. He is author, contributing author, or co-translator of several books, including the monographs Dark Passages of the Bible: Engaging Scripture with Benedict XVI and Thomas Aquinas and Jesus, Interpreted: Benedict XVI, Bart Ehrman, and the Historical Truth of the Gospels. Dr. Ramage's work has appeared in a number of scholarly journals including Nova et Vetera, Scripta Theologica, Cithara, and Homiletic and Pastoral Review as well as popular online venues such as Strange Notions, The Gregorian Institute, and Crisis. He has been interviewed by news outlets such as the National Catholic Register andFirst Things and has made periodic appearances on the EWTN programs Catholic Answers Live, Catholicism on Campus, and The Son Rise Morning Show. See https://www.catholic.com/audio/cal/8360#.WlmNNGt_vzAFor more on his work and his CV, visit Dr. Ramage's website www.truthincharity.com.

Für die stabile, regulierte Expression eines therapeutischen Gens in Zielzellen stellen künstliche menschliche Chromosomen (HACs) derzeit das einzige Konzept dar, das hohe Sicherheit und technische Realisierbarkeit bietet. Künstliche Chromosomen (HACs) mit dem CFTR-Gen sollen sich durch den Transfer definierter HAC-Konstrukte effizient formieren, ohne dabei die Zielzellen zu stören. Im Augenblick werden künstliche Chromosomen zur Klärung vieler grundlegender Fragen auf dem Gebiet der Chromosomenstruktur und -funktion sowie der Genregulation konstruiert. In der vorliegenden Arbeit sollte die zugrundeliegende de novo HAC-Technologie der Arbeitsgruppe, die sich auf die Konstruktion und den intakten Transfer von PACs mit den funktionellen Komponenten menschlicher Chromosomen (zentromerische, telomerische und genomische Sequenzen mit oris und Gene) konzentriert, weiterentwickelt werden. Die Verwendung von PACs als Kloniervektoren erlaubt die stabile Klonierung langer, genomischer DNA, die biochemische Verknüpfung über lox/Cre vermittelte Rekombination, sowie die Darstellung grosser Mengen intakter, supercoiled PAC DNA durch Anwendung einer in der Arbeitsgruppe entwickelten Technik (Reinigung der supercoiled DNA Fraktion in Agaroseplugs von gebrochener und genickter DNA im elektrischen Feld). Eine Verbesserung der Vektoren ist nötig, da die Effizienz der HAC Formierung bisheriger Vektoren für eine klinische Anwendung nicht ausreicht. Dafür wurden zunächst Marker benötigt, die Anzeigen können wieviele Zellen mit wievielen Vektormolekülen transfiziert wurden und wieviele der physikalisch erfolgreich transfizierten Zellen stabile HACs bilden (genetische Funktion). Im Rahmen dieser Arbeit wurden Expressionsmarker entwickelt, die eine Formierung stabiler HAC-Linien durch grüne Fluoreszenz anzeigen können. So wurde ein tetratelomerischer PAC-Vektor „pTT“ konstruiert, der stabil eine EGFP-Kassette exprimiert, ein funktionelles Zentromer trägt (TTE1), und für die Klonierung weiterer genomischer Komponenten eine weiss/blau selektionierbare Sal I Stelle enthält. Ausserdem wurde ein CFTR-Gen-basierender „genomischer“ Marker (159 kb) vorgestellt, der den intakten Transfer langer, genomischer DNA und die Expression vom CFTR Promoter anzeigen kann. Besonders hervorzuhebende Ergebnisse aus der Arbeit sind: 1) Kopiezahlabhängigkeit bei der transienten Expression. Einzelne Markergen-Kopien genügen nicht, um Anwesenheit der transfizierten DNA mittels transienter Expression nachzuweisen. 2) Sichtbar transient exprimierende HAC Konstrukte (große Zahl) führen nicht zu stabilen Linien, was nahelegt, dass ein „low copy“ HAC Transfer benötigt wird. Für eine Optimierung und besseres „low copy“ HAC-Monitoring wurden multimere Marker (EGFP Array) und DNA-tags (Gal4-BD, Lac-Operator) entwickelt und stehen nun für einen Einsatz bereit. 3) Für den „low copy“ Transfer wurden neben Lipofektionsassays und der Mikroinjektion insbesondere eine neue Methode, die Baktofektion, eingesetzt, bei der die DNA nicht aus Bakterien isoliert werden muss: Modifizierte Transferbakterien dringen in die Zelle ein und geben die DNA-Konstrukte nach Autolyse frei („suicidal transfer“). Dabei wurde ein funktioneller Transfer genomischer DNA nachgewiesen. Es konnte zum einen gezeigt werden, dass Zentromer tragende Konstrukte effizient de novo HACs bildeten, und zum anderen, dass das lange genomische CFTR Expressionskonstrukt CGT21 stabil vom CFTR Promoter exprimiert wird. Damit steht nun die Baktofektion als effizienteste Methode zur HAC Optimierung zur Verfügung. 4) Durch Auszählung der stabilen Klone, Isolierung von Stichproben klonaler Linien und einem HAC-Formierungsassay mittels FISH Analyse, wurden folgende grundlegende Beobachtungen gemacht: Die Rate der Formierung stabiler Klone mit HAC Konstrukten hängt nicht von a) der Zahl der im einzelnen Konstrukt vorhandenen, oder cotransfizierten Zahl der BS Marker, b) nicht von der Orientierung der alpha-sat DNA relativ zum BS-Gen oder dem entgegengesetzt gerichteten EGFP-Gen, und c) nicht absolut von der Verwendung unterschiedlicher alpha-sat Sequenzen der zwei homogenen Array Typen auf Chr.5 ab, wobei der Vektor pTT mit dem Zentromer E1 die besten Ergebnisse der HAC Bildung erzielte und die höchsten Klonzahlen in Cotransfektionen mit einem telomerisierten Genkonstrukt erhalten wurden, nicht aber in Cotransfektionen mit dem Telomervektor ohne einklonierte genomische DNA. Diese Erkenntnisse haben direkte Relevanz für die Weiterentwicklung CFTR exprimierender HAC Vektoren. Mit den Konstrukten pTTE1 und CGT21, und den zukünftig erweiterten Konstrukten mit multimeren Markern bzw. Tags und einem kompletten CFTR Gen, kann nun auch der physikalische Transfer der HAC Konstrukte in Zielzellen, sowie deren Funktion effizient untersucht werden. Damit konnten wichtige Voraussetzungen für die Weiterentwicklung einer stabilen CFTR Gentherapie geschaffen werden.

Human artificial chromosomes (HACs) were generated by transfer of telomerized PAC constructs containing alpha satellite DNA of various human chromosomes. To monitor which cells took up constructs and subsequently formed stable clones under blasticidin S (BS) selection, a CMV/EGFP expression cassette was inserted into a HAC construct based on chromosome 5 alpha satellite DNA (142 kb). Lipofection into HT1080 cells resulted in a small proportion of cells exhibiting bright green fluorescence on day 1. Areas containing such early green cells were marked, and plates monitored over 2 weeks. In only one out of 41 marked areas, a viable clone developed. In the remaining 40 areas, the green cells ceased division at 1-8 cells. In contrast, outside the marked areas, 16 stable clones formed which did not exhibit green fluorescence during the first cell divisions, but all cells of each became green around day 4 -6. Fluorescence in situ hybridization (FISH) analysis of isolated clonal lines demonstrated low copy HAC formation without integration. We conclude that transient expression of an EGFP marker on HAC DNA is not a suitable means for the identification of the proportion of transfected cells which are capable of forming viable clones. One explanation could be that the high copy number required to consistently detect transient EGFP expression (Schindelhauer and Laner, 2002) impairs viability and clone formation. Copyright (C) 2004 S. Karger AG, Basel.