Podcasts about painfully

Send us a textWhy is it that the tiniest injuries hurt the MOST? In this episode, we break down the dumbest, most annoying, and surprisingly painful injuries that life throws at you—stubbed toes, paper cuts, mystery bruises, and more. These aren't heroic battle scars... they're “I bumped into the coffee table again” injuries. Relatable? Painfully.Grab a drink, cringe with us, and share your own ridiculous injury stories in the comments. Let's bond over the pain that shouldn't have hurt this bad.One Drink PodcastOne topic for One Drink. Topics are different and fun unlike the main media.Gods N GladiatorsCustom Clothing / Wall ArtDisclaimer: This post contains affiliate links. If you make a purchase, I may receive a commission at no extra cost to you.https://www.youtube.com/channel/UC-ytHeiGG6VND5GUmoWij-A

You're not going to like this—but I've come to believe it's true:We're not teaching people empathy.Not in business school.Not in PhD programs.Not in most classrooms.We're teaching performance.Frameworks.Strategy.Execution.But not how to read a room.Not how to ask:“How is this person actually feeling right now?”Or: “How do I show up in a way that helps, not harms?”I used to think empathy was soft.I now believe it's the core skill that makes or breaks your success—in research, in business, and in life.The thing is… I'm naturally empathetic.And for a long time, I assumed everyone else was too.That people were good.That they meant well.That if I showed up with kindness, it would be returned.That was wrong. Painfully wrong.Empathy doesn't work on everyone.Some people take advantage of it.Some people weaponize your kindness.And the more you give, the more they take.But that doesn't mean you give up on empathy.It means you learn to use it wisely.Empathy is not weakness.It's not being a doormat.It's not endlessly giving.It's knowing when to give.And when to walk away.In my house, we talk about feelings.We ask, “How do you think they felt?”And I've seen the difference that makes with my kids.A little empathy changes everything:How you teach.How you parent.How you lead.How you breathe in a room that doesn't fully accept you.But here's the catch:Empathy only works if you also protect yourself.So if you're the kind of person who gives…Who senses everything…Who gets crushed when you're ignored or dismissed—This is your permission:You are not wrong. You are not soft. You are not naive.You're reading the world at a deeper level.Just make sure you don't burn out trying to fix it.Empathy is your superpower.But like all power, it needs boundaries.

If you're waiting for a breakthrough moment, a sign from the universe, or the “perfect” conditions to create change… this episode is your wake-up call.Today I share with you the actual framework I use (and teach) to create meaningful, long-lasting change. No fluff. No hype. Just reality, honesty, and the painfully simple steps we often avoid because they seem too obvious or not enough.Here's what we cover:Why change starts with meeting life exactly as it is.The power of looking back—not to dwell, but to understand how you actually got here.Looking forward—not with fantasy, but clarity on what you'd love life to feel and look like.The unsexy magic of taking the next simple step. Then the next. Then the next.Why playing the long game matters way more than chasing quick wins.If you've been feeling stuck, overwhelmed, or like change is too hard—get this episode in your earballs. Because the truth is, it's not that complicated. It's just uncomfortable. And most of us would rather stay in a known discomfort than face the unknown of change.But if you're willing to show up, get honest, and take those painfully simple steps, everything shifts.Buy my book, Authentic - coming home to your true self - AUS, EUR, USACoach with me 1:1 - Book a 20-minute call nowThe Good Life - sign up nowFree Wheel of Life Assessment - take the test nowSunday Meds - live event by the beach, June 22ndWrite into us - let us know what you want to hearwww.katjohn.com.auSupport the show

Here is a narration of my latest ‘a calm presence', inspired by this quote from Indy Johar's May 12th, 2025 Substack posting, The Stickiness of Want  - And the Systemic Amnesia Behind It :We—you, me, everyone in this room—are the last generation with viable agency before degenerative volatility locks us into conflict and collapse. The window is painfully small but gloriously open.'This posting was written while traveling in India and Japan in April and May of 2025.The narrated version was recorded in one take on May 21, 2025 on the streets of Hakone-Yumoto, Japan with the Haya River and lively birdsong in the background.See the Transcript of this episode for the complete posting.  *END NOTES FOR ALL EPISODESHey conscient listeners, I've been producing the conscient podcast as a learning and unlearning journey since May 2020 on un-ceded Anishinaabe Algonquin territory (Ottawa). It's my way to give back.In parallel with the production of the conscient podcast and its francophone counterpart, balado conscient, I publish a Substack newsletter called ‘a calm presence' see https://acalmpresence.substack.com. Your feedback is always welcome at claude@conscient.ca and/or on social media: Facebook, Instagram, Linkedin, Threads or BlueSky.I am grateful and accountable to the earth and the human labour that provided me with the privilege of producing this podcast, including the toxic materials and extractive processes behind the computers, recorders, transportation systems and infrastructure that made this production possible. Claude SchryerLatest update on March 26, 2025

Join this channel to get access to perks:https://www.youtube.com/channel/UCuvjQYKukKjVyhSVxQibgOw/joinFollow Us!https://www.instagram.com/calumjohnson1/https://x.com/calum_johnson9https://www.youtube.com/@UCf8gbZ4ZyNcc9WIOOEEfpSQ Timestamps00:00 Intro04:48 What makes Malique's message resonate so deeply07:16 “She went without so we could have” (A Mother's Sacrifice!!)16:24 The 300 job rejections — and the moment that changed everything18:25 “No one hired me, so I hired myself”22:40 The first viral video: 100K views in 24 Hours!24:47 Step 1: Wake up — God wants you see this!31:42 How to know you're asleep at the wheel36:26 Signs you're living someone else's dream38:53 Your mom will need you one day — and you must be ready44:09 The real reason your life isn't changing47:31 Step 2: Self-audit and get still50:59 You can't hear God if you're never quiet53:33 How journaling & prayer unlock your purpose58:49 Step 3:"Go Ghost: Upgrade Your Life In Silence" (this will change everything!)1:01:15 Why you must protect your dreams in the early stages1:13:34 Step 4: Build your discipline code (habits, systems, momentum)1:22:45 The 10% rule: how to win on your worst days1:28:08 Why the gym is the most important discipline1:34:19 Identity shift: "I am purpose now"1:38:23 “Life is not about what we achieve. It's about what we overcome.”About The GuestMalique David Lewis is a speaker, creator, and founder of 'Purpose Driven'—a movement empowering men to live with intention, discipline, and faith. From poverty in Miami to building a platform with millions of views, his story is one of spiritual clarity, community leadership, and relentless self-belief.

Post-Dispatch sportswriter Tom Timmermann joined columnist Jeff Gordon to discuss the agonizing end to the Blues-Jets series, but also the better times to come. Find more podcasts from the Post-Dispatch. Subscribe to the Post-Dispatch.

(00:00) The crew recaps the Celtics' Game 4 win over the Magic, highlighting Jayson Tatum's big performance and the team's physical play. McKone feels the Celtics should handle the series more easily, while Beetle calls Tuesday’s Game 5 a must-win to take control. (6:37) Beetle says the Magic have controlled much of the first-round series, calling Game 4 a statement win for the Celtics after the frustrating Game 3 loss. He adds they could have swept the series, but now it’s become more difficult. The crew also breaks down Jaylen Brown’s performance over the last two games. (17:02) The guys discuss Bill Belichick’s CBS interview, focusing on his comments about not mentioning Robert Kraft in his book and the conflicting accounts of his departure from New England. They also touch on Jordon Hudson avoiding a question about how she and Belichick met. (29:14) The guys continue dissecting the CBS interview, zeroing in on Bill Belichick’s relationship with Jordon Hudson and questioning why both declined to answer how they met. They speculate on the secrecy and what it might suggest about the nature of their relationship.

Please Subscribe + Rate & Review KMJ’s Afternoon Drive with Philip Teresi & E. Curtis Johnson wherever you listen! --- KMJ’s Afternoon Drive with Philip Teresi & E. Curtis Johnson is available on the KMJNOW app, Apple Podcasts, Spotify, Amazon Music or wherever else you listen. --- Philip Teresi & E. Curtis Johnson – KMJ’s Afternoon Drive Weekdays 2-6 PM Pacific on News/Talk 580 & 105.9 KMJ DriveKMJ.com | Podcast | Facebook | X | Instagram --- Everything KMJ: kmjnow.com | Streaming | Podcasts | Facebook | X | Instagram See omnystudio.com/listener for privacy information.

Please Subscribe + Rate & Review KMJ’s Afternoon Drive with Philip Teresi & E. Curtis Johnson wherever you listen! --- KMJ’s Afternoon Drive with Philip Teresi & E. Curtis Johnson is available on the KMJNOW app, Apple Podcasts, Spotify, Amazon Music or wherever else you listen. --- Philip Teresi & E. Curtis Johnson – KMJ’s Afternoon Drive Weekdays 2-6 PM Pacific on News/Talk 580 & 105.9 KMJ DriveKMJ.com | Podcast | Facebook | X | Instagram --- Everything KMJ: kmjnow.com | Streaming | Podcasts | Facebook | X | Instagram See omnystudio.com/listener for privacy information.

Join this channel to get access to perks:https://www.youtube.com/channel/UCuvjQYKukKjVyhSVxQibgOw/joinGet 10% off your first month https://betterhelp.com/calumjohnsonStart investing safely with Public https://public.com/calumTry notion for free https://notion.com/calumjohnsonOur Merch drops soon! Be 1 of 50 to get EXCLUSIVE ACCESS: https://bit.ly/StayDelusional2 Follow Us!Instagram: https://www.instagram.com/calumjohnson1?igsh=MTdzbHI1b3c1b2dmag== Twitter https://x.com/calum_johnson9?s=21 Ashley Fox https://www.instagram.com/_ashleymfox?igsh=aW1paWVkcHdlNTB1Watch our episode with Donni Wiggins on how to get rich in 2025 https://youtu.be/GkxGipg1EEM00:00 Intro05:38 Most limitations are imaginary. 08:37 Imposter syndrome on Wall Street!13:01 Your environment determines your dreams 16:30 How wealthy people handle finance25:13 Difference in mindsets between the wealthy and the 99%30:35 How therapy changed her life35:59 I spent $100,000s to heal my trauma44:06 Allow yourself to heal 47:45 Don't just survive, thrive!52:20 How to create your roadmap for success59:56 How to be rich in this Trump economy1:05:00 Breakdown of how the stock market works1:10:00 Easiest way to choose stocks to buy 1:14:46 Full research process to pick the right stocks to invest in1:19:26 Red flags to check for before buying stocks 1:25:17 Stop panic selling1:32:40 Programming yourself for your first million 1:35:11 Separate your identity from your business 1:47:02 You can come back from anything About the video Today's guest is Ashley Fox. She's a financial educator, entrepreneur, and founder of Empify. In this episode, she shares her journey of overcoming self-doubt, healing through therapy, and reprogramming her mindset to unlock abundance. She also breaks down how to understand the stock market, start investing as a beginner and program yourself to make your first million. This is more than investment talk — it's a blueprint for healing and thriving.Public Disclosures: All investing involves the risk of loss, including loss of principal. Brokerage services for US-listed, registered securities, options and bonds in a self-directed account are offered by Public Investing, Inc., member FINRA & SIPC. Public Investing offers a High-Yield Cash Account where funds from this account are automatically deposited into partner banks where they earn interest and are eligible for FDIC insurance; Public Investing is not a bank. Cryptocurrency trading services are offered by Bakkt Crypto Solutions, LLC (NMLS ID 1890144), which is licensed to engage in virtual currency business activity by the NYSDFS. Cryptocurrency is highly speculative, involves a high degree of risk, and has the potential for loss of the entire amount of an investment. Cryptocurrency holdings are not protected by the FDIC or SIPC. A Bond Account is a self-directed brokerage account with Public Investing, member FINRA/SIPC. Deposits into this account are used to purchase 10 investment-grade and high-yield bonds. The 6%+ yield is the average, annualized yield to worst (YTW) across all ten bonds in the Bond Account, before fees, as of 12/13/2024. A bond's yield is a function of its market price, which can fluctuate; therefore, a bond's YTW is not “locked in” until the bond is purchased, and your yield at time of purchase may be different from the yield shown here. The “locked in” YTW is not guaranteed; you may receive less than the YTW of the bonds in the Bond Account if you sell any of the bonds before maturity or if the issuer defaults on the bond. Public Investing charges a markup on each bond trade. See our Fee Schedule. *Terms and Conditions apply.

Join Snaxton & Goose as they discuss current events, The Righteous Gemstones, and the life of a painfully average man. Don't forget to rate review and subscribe!

Question: What was the position of 19th century American Jews to the Civil War and Slavery? Answer: Complicated. Very complicated.Painfully and, in some ways, shamefully complicated, according to the historian Richard Kreitner. In his new book, Fear No Pharaoh, Kreitner explores the radically diverse positions that American Jews held toward slavery during the Civil War. He highlights 6 prominent Jewish figures including Judah Benjamin (a Confederate leader), Rabbi Morris Jacob Raphael (who justified slavery using Torah), David Einhorn (an abolitionist rabbi), Isaac Mayer Wise (who advised Jews to stay out of the conflict), August Bondy (who fought with John Brown), and Ernestine Rose (a radical feminist activist). Kreitner explains how American Jews, numbering around 150,000 by 1860, were - like the rest of the (dis)United States - deeply divided on slavery, with most influenced by regional issues that usurped the supposedly universalist religious ethic of their faith. 5 KEEN ON AMERICA TAKEAWAYS * American Jews were deeply divided on slavery and the Civil War, with most adopting the political views of their geographic region rather than having a unified "Jewish position."* The Jewish experience with slavery in Egypt (celebrated in the Passover tradition) created a complex dynamic for American Jews confronting American slavery, with some using it to oppose slavery while others justified the practice.* Jewish figures like Judah Benjamin rose to high positions in the Confederacy, while others like Rabbi David Einhorn were forced to flee for their anti-slavery activism.* Anti-Semitism was relatively subdued in the American South before the Civil War (as Black enslavement served as the primary social hierarchy), but increased during and after the war.* Figures like Ernestine Rose represented an intersection of Jewish identity, abolitionism, women's rights activism, and freethinking, highlighting the diverse ways American Jews engaged with 19th century social reform movements.Richard Kreitner is the author of Break It Up: Secession, Division, and the Secret History of America's Imperfect Union and Booked: A Traveler's Guide to Literary Locations Around the World. He has written for The New York Times, The Washington Post, The Boston Globe, The Nation, Slate, Raritan, The Baffler, and other publications. He lives in the Hudson Valley, New York. In his new Substack podcast, Think Back, Kreitner interview US historians about connections between the past and the present.Named as one of the "100 most connected men" by GQ magazine, Andrew Keen is amongst the world's best known broadcasters and commentators. In addition to presenting the daily KEEN ON show, he is the host of the long-running How To Fix Democracy interview series. He is also the author of four prescient books about digital technology: CULT OF THE AMATEUR, DIGITAL VERTIGO, THE INTERNET IS NOT THE ANSWER and HOW TO FIX THE FUTURE. Andrew lives in San Francisco, is married to Cassandra Knight, Google's VP of Litigation & Discovery, and has two grown children. This is a public episode. If you'd like to discuss this with other subscribers or get access to bonus episodes, visit keenon.substack.com/subscribe

Join this channel to get access to perks:https://www.youtube.com/channel/UCuvjQYKukKjVyhSVxQibgOw/joinSecure your business with $1000 off at Vanta: https://vanta.com/calum Start investing safely with Public: https://public.com/calumOur Merch drops soon! Be 1 of 50 to get EXCLUSIVE ACCESS: https://bit.ly/StayDelusional2  Follow Us!Instagram: https://www.instagram.com/calumjohnson1?igsh=MTdzbHI1b3c1b2dmag== Twitter https://x.com/calum_johnson9?s=21 Greg Isenberg: https://x.com/gregisenberg?s=21Watch our episode with Dom Ashburn on how to get 100k followers in 30 days using AI https://youtu.be/cai40ROkTYM00:00 Intro02:07 AI would change your life!03:10 What is an AI agent?08:07 2 business models to create with smart software 12:19 How you can build a $1M AI startup in 6 months. 17:55 How to use social media to build an AI business24:43 Why he dropped out of college 28:00 How to pick AI business ideas to work on. 36:39 Best time to launch your product 41:47 How to set up a business in 24 hours using AI agents (Live crash course) 55:29 How to turn your product into a movement 57:43 4 step playbook to build an audience in 20251:02:36 How to keep your users coming back 1:09:13 Why you MUST focus on the core loop1:11:10 The blueprint to partner with influencers. 1:14:57 How to build relationships with successful people About the videoToday's guest is Greg Isenberg — a startup entrepreneur, advisor, and CEO of Late Checkout, a company that builds and invests in internet communities. In this episode, he breaks down how AI is reshaping entrepreneurship and shares the exact playbook to build a $1M AI startup in just 6 months; from idea creation to distribution to customer retention — this is your blueprint for winning in 2025.Public Disclosures: All investing involves the risk of loss, including loss of principal. Brokerage services for US-listed, registered securities, options and bonds in a self-directed account are offered by Public Investing, Inc., member FINRA & SIPC. Public Investing offers a High-Yield Cash Account where funds from this account are automatically deposited into partner banks where they earn interest and are eligible for FDIC insurance; Public Investing is not a bank. Cryptocurrency trading services are offered by Bakkt Crypto Solutions, LLC (NMLS ID 1890144), which is licensed to engage in virtual currency business activity by the NYSDFS. Cryptocurrency is highly speculative, involves a high degree of risk, and has the potential for loss of the entire amount of an investment. Cryptocurrency holdings are not protected by the FDIC or SIPC. A Bond Account is a self-directed brokerage account with Public Investing, member FINRA/SIPC. Deposits into this account are used to purchase 10 investment-grade and high-yield bonds. The 6%+ yield is the average, annualized yield to worst (YTW) across all ten bonds in the Bond Account, before fees, as of 12/13/2024. A bond's yield is a function of its market price, which can fluctuate; therefore, a bond's YTW is not “locked in” until the bond is purchased, and your yield at time of purchase may be different from the yield shown here. The “locked in” YTW is not guaranteed; you may receive less than the YTW of the bonds in the Bond Account if you sell any of the bonds before maturity or if the issuer defaults on the bond. Public Investing charges a markup on each bond trade. See our Fee Schedule. *Terms and Conditions apply.

Dive into the world of choral music publishing with this episode featuring Jack Zaino from Alfred Music! Gain insights into Alfred's focus on serving public schools, teachers, students, and community choirs. Aspiring composers will discover essential advice on submitting music and building industry connections. Choral directors will find valuable tips for selecting the perfect repertoire. Explore the collaborative nature of music publishing and hear Jack's reflections on his mentor, Audrey Snyder. A must-listen for anyone involved in the choral music community.

QOTD: Who or what were you painfully wrong about?

The Texans are unlikely to add another starter FA. How do we feel about that? Are you ready to scrap the ‘Tuve in LF experiment? QOTD: Who or what were you painfully wrong about?

In hour one, Crowder wants Appel to know he doesn't respect his wardrobe decisions. Messi not selected for Argentina's national team - bad news for Inter Miami? Crowder pumps his chest over SEC dominance in the NCAA bracket. Plus, Will Manso joins the show to discuss the Heat's losing streak and big picture decisions that loom over the final 15 games of the season.

Today on the Matt Walsh Show, Gavin Newsom pretends to be moderate as he prepares for his inevitable 2028 presidential run. One of the astronauts trapped on the International Space Station confirms Elon Musk's claim that the Biden Administration wouldn't let Musk rescue them. We have another cultural appropriation controversy—haven't had one of those in a while. And a newly unearthed video shows that Representative Jasmine Crockett used to speak like a normal person of at least average intelligence before she was elected to Congress. Click here to join the member-exclusive portion of my show: https://bit.ly/4bEQDy6 Ep.1551 - - - DailyWire+: We're leading the charge again and launching a full-scale push for justice. Go to https://PardonDerek.com right now and sign the petition. Now is the time to join the fight. Watch the hit movies, documentaries, and series reshaping our culture. Go to https://dailywire.com/subscribe today. Get your Matt Walsh flannel here: https://bit.ly/3EbNwyj - - - Today's Sponsors: Grand Canyon University. - Find your purpose at Grand Canyon University. Visit https://gcu.edu today. Policygenius - Head to https://policygenius.com/WALSH to get your free life insurance quotes and see how much you could save. Renewal by Andersen - Text WALSH to 400-400 for a FREE consultation to save $379 off every window and $779 off every door. - - - Socials:  Follow on Twitter: https://bit.ly/3Rv1VeF Follow on Instagram: https://bit.ly/3KZC3oA Follow on Facebook: https://bit.ly/3eBKjiA Subscribe on YouTube: https://bit.ly/3RQp4rs

The 2025 Oscars were held in Hollywood Sunday night and broadcast on ABC. Conan O'Brien hosted the Oscars...but the greatness of Conan O'Brien couldn't overcome what was a painfully boring show. We review and react to the 2025 Academy Awards. We discuss the largely unknown actors and actresses that won Oscars Sunday night. We discuss the decline of Hollywood awards shows...and how shows like the Oscars are becoming irrelevant. We explain how entertainment has become a niche industry...and how it's led to the decline of the Oscars and other awards shows. USE PROMO CODE BTL20 TO SAVE 20% WITH SUGAR MOUNTAIN TRADING: https://sugarmountaintrading.com

Arsenal trudged out of the City Ground with a 0-0 stalemate against Nottingham Forest—a result that felt like swallowing bitter medicine. Defensively resolute?Absolutely. Offensively forgettable? Painfully so. Arsenal's backline stood lively against Forest's physicality, but their attack sputtered like a car out of fuel, mounting on frustration as we count the days and weeks. The first half was a few memorable displays. Saliba, Gabriel, and Timber—operated like a fortified wall, snuffing out Chris Wood's threats. But up front, Merino's false-nine experiment worked...and worked, beyond recycled possession and hopeful crosses into a forest of defenders.  Bright spots flickered briefly: Tierney's introduction sparked width, forcing a sharp save from Sels, while Ødegaard's late blocked shot was simply would-be-victory. Yet Forest's low block proved impenetrable, reducing Arsenal to sideways passes and speculative efforts. The clean sheet? Laudable. The attacking inertia? We all know.   This wasn't a disaster, but we have a vacancy for hero. A point earned is fair. But we need to strive for more. Arguably. Tune in for byte-size breakdown.  Share this episode with fellow Gooners. Onwards, but are in it during these hard times. C'mon Arsenal!

After a world-wide disaster, new rules take effect.Based on a post by barnabus, in 2 parts. Listen to the Podcast at Steamy Stories.Forward:The setting is the nineteen fifties. After a world-wide disaster, new rules take effect.Jolene; A high school cheer leader, awakens to find that a worldwide disaster has occurred killing 85% of the world's population. She has been selected to participate in a breeding program to replenish the world's population. Most of the participants are virgins. It also explains Jolene's surprise when she hears a girl moaning with sexual pleasure and Jim's shock when Jolene becomes 'the aggressive partner' in their relationship. (For those who grew up after the 'free love' period of the 1960s and 1970s, you'll just have to take my word for it: That's the way it was!)Nanette, on the other hand, has obviously been 'sexually active' (to use a modern term: In the 1950s, another description would have been used), but it becomes apparent to the astute reader that although she has been sexually 'active', she probably has not received a great deal of satisfaction from her sexual activity.Chapter 1: Orientation."As you all know, there was a world-wide disaster."As if coming out of a daydream, Jolene's mind drifted back into reality. The last thing she remembered was sitting in a Trigonometry class thinking about a cheer leading routine. She was the head cheerleader and was scheduled to run the next cheer leading practice.There was a man with a clipboard and a briefcase at the head of a long table, talking. Instinctively, Jolene tuned in on what he was saying.The word "disaster" grabbed her attention. He continued: "To our best estimate, as much as 85% of mankind has been destroyed." Jolene was suddenly awake and alert, carefully listening. He went on, "The government, what's left of it, has determined that an organized breeding program must be established, and you have all been selected to be part of the initial phase. Everyone in this group is 18 years old and was a high school student. You're all in good health, and hopefully, you will be able to produce healthy offspring. The girls are all in their fertile period, so this is an ideal time for each of you. You have been pared with a partner who is a favorable genetic match to you, and you have been brought here to see if you are compatible with your match. And if you are, you will be expected to breed to procreate children to help repopulate our country and world."Jolene looked around the room. Everyone was about her age, except the man at the head of the table. They were arranged in boy-girl couples. Jolene recognized two of the girls in the room: she was sitting next to Jim and across the table, Jolene recognized Nanette, a close girlfriend, was sitting next to John. Jim and John were both nerds and not part of Jolene's 'inner circle' of friends. She had seen the another girl and boy from her school in the halls and knew they were a couple, but she didn't know their names. And there were other couples that Jolene didn't know, apparently from other schools.Nanette wore a new, scandalously fashionable see-through blouse which displayed her lacy camisole top that hugged her well-shaped breasts and revealed the lines of Nanette's brassier The bra-straps were clearly visible. There was just a trace of two bumps showing exactly where Nanette's nipples were beneath her undergarments. Nan's skirt was the shortest skirt in the room, stopping slightly above her knees: rather immodest for the mid 1950's.¬All of the girls were wearing blouses that buttoned down the front and skirts. There were no sweaters or slacks.Jolene raised her hand. "Excuse me. What disaster? I don't know about any disaster. The last thing I remember was sitting in Trigonometry class at school. What are you talking about?""That is a normal after effect of the disaster. The disaster occurred several weeks ago. Many people do not remember either the disaster or what happened afterward. At some point, your memory will begin working again and will pick up from that point onward. I'm sorry, Miss," he paused to look at his clipboard, "Miss Jolene, if your memory hasn't begun functioning yet. And in case you don't remember, I am the monitor of this program."He scanned the others in the room. "Are there others that don't remember what has happened before today?" he asked. Several hands in the room went up. "This must be terribly frightening for you."Jolene and several others nodded."I'm sorry that you are finding yourself in this situation without knowing what led up to it. However, it has been decided we cannot delay the program to increase our population any longer." He looked around the room, meeting the many nervous eyes in the room. "I appreciate that this might be especially difficult for many of you. In this world of the 1950's most of you young people live celibate lives, and although you might think about sex, probably most of you have never", he paused and stammered, "taken your fantasies to, um, shall we say" he paused to swallow, "their ultimate conclusion."Many of the young people, especially the girls, were embarrassed the casual way he was talking about their sex-lives; or, rather, the lack of sex in their lives.Again, Jolene raised her hand. "Excuse me, but shouldn't I be matched up with Tom Green?" she asked. Tom was her boyfriend. He was a year older than she was and they had been dating for a little more than a year."Green?" The man asked and pulled a large book from his briefcase. He perused the pages, then looked up sadly. "I'm afraid Tom didn't survive the disaster. I'm sorry, Miss Jolene" He returned his book to his briefcase. "In any case, we believe that, er," he fumbled with a name and pointed toward Jim, the boy sitting next to Jolene, "that he is the best genetic match for you that is available."Jolene gave Jim a withering sideways glance.Resuming his lecture, the man continued, "Each couple will be given its own room, which will be adequately furnished. We would like to get the program started as soon as possible." There was a moment of embarrassed silence in the room before the monitor added: "I know that many of you are embarrassed by this, but let me assure you that there are boys and girls all around the country are meeting in rooms like this and are being told the same things you are. Many are meeting their prospective mates for the very first time. I believe you all have the advantage of already being acquainted with the mate that has been selected for you."A pretty girl, the one from her school that Jolene didn't know, raised her hand. "But I can't do this. I'm not married to; " she pointed to the boy sitting next to her."We chose to start this program without requiring marriage," the monitor stated. "If it turns out that your offspring match our genetic expectations and you wish to get married to your partner, then of course you can. But we'd like to keep the option of rearranging the couples if the genetics don't work out the way we hope they will. We need to replenish our population as rapidly as feasible and to expand our gene pool as widely as possible. Therefore, every girl of childbearing age will be expected to have at least four to six children. Ideally, each child will be sired by a different father since that will give us the widest possible diversity to our gene pool. So for the moment, marriage is not required. If you wish to be married before you proceed, we can furnish you with an appropriate clergyman.The girl who had raised the question looked at the boy sitting next to her, and shook her head. "No, I think marriage can wait." Obviously, although she had been dating her 'mate', she wasn't ready to be married to him, yet. She probably also was not enamored with the idea of deliberately having a baby with him. Or possibly it was simply going through the process of starting a baby that she didn't look forward to.Again, Jolene raised her hand. "I'm only eighteen. That's awfully young to have a baby, or to start a family," she stated."The world is different now," the monitor stated. "Some of the old principles are not practical anymore. This program starts with 18-year-olds and above, but soon, probably even younger girls will be encouraged to have babies."Considering the discussion closed, the monitor concluded his remarks."All right, then. We will take each couple to its room and we will allow you to proceed. Each room is equipped with a sofa and a bed and bathroom facilities. There is a phone in each room. Simply pick up the phone if any of you need help or encouragement. We're here to help you, and we'd like to make things as easy as possible for you."He stood and started toward the door. "Oh, I know this may be difficult for many of you. I suggest that once you are in your room, girls should open their blouses and remove their bras and panties. Boys should remove their trousers and unbutton your shirts. This may help encourage the mood."Also, remember, our goal is for each girl to get pregnant as soon as possible. Therefore, we encourage each of you to copulate several times to increase your odds of getting pregnant. He paused and faced the group with a smile that somehow seemed inappropriate."And now," he said officiously, "be fruitful and multiply."Chapter 2: Getting started.The room had the sterile feel of a hotel room. There were no windows, and Jolene remembered there had not been a window in the room where they had their 'orientation'.Painfully self-consciously, both Jim and Jolene turned away from each other to remove the required garments.When they turned to face each other, both quickly averted their eyes after a quick, curious glance. Jolene was relieved that even though Jim had removed his pants, he had the presence of mind to keep one button on his shirt closed. Thus his shirt hung down far enough to conceal his male anatomy.Jim's glance revealed that although her blouse was open, either she wasn't wearing a bra (something that he had suspected when he had seen her walking around school with an eye-catching bounce) or it had been one of these open-in-the-front bras and she had already opened it. Of course, her skirt hung down revealing nothing improper.Then, as if by mutual agreement they both sat on the sofa facing each other. The sofa was wide enough that Jim could rest his arm on the back behind Jolene's shoulders, but there was still a small distance separating them.Both teen were embarrassed and uncomfortable. And both were afraid to speak the first words.Jolene couldn't imagine how she could be in a hotel room with a boy she scarcely knew. Jolene had good grades in school, was head cheerleader, was planning to attend college. She came from a well-to-do family who lived in a good neighborhood in a big house.She had always known she would give up her virginity someday. Having been raised with the idea of 'saving herself' for her wedding night, she had assumed it would be when she got married. But Tom, her boyfriend, had been pressuring her during the past couple of months. Tom's pressure had led her to question the validity of waiting and she had been seriously considering 'doing it' with Tom.After all, Tom had argued, the hymen was just a small piece of membrane: it's presence or absence is totally invisible to the external eye. In fact, some girls don't even have one. And some have lost it riding bicycles or in some other innocuous way.And if Jolene married someone other than Tom, and virginity was important to her husband, Tom reasoned, Jolene could always assure her husband that he was the first one and her husband would never know the difference.But now, if she was going to have a baby, virginity was really a moot point.Jim was a relative stranger to her. She had seen him in the halls at school and they had spoken briefly, but she really knew very little about him.'It's not the right time of the month for me to get pregnant', Jolene thought. But with a start she realized that apparently some time had passed since the 'disaster', whatever the 'disaster' had been. Undoubtedly, she was at a different place in her cycle than she remembered.Without a word, Jim slipped the band from her head that held her hair in place. Then slowly, one at a time, he removed her hairpins, allowing her hair to fall over her shoulders. When the last pin was removed, Jolene shook her hair loose, allowing it to fall naturally over her shoulders.Finally, Jolene looked into Jim's eyes, her face filled with fear and concern."I guess we're lab partners and we've been given a homework assignment," she stated flatly. "Do you think we should start?" she asked, and, although Jim had only seen the slight movement of her arm, he felt her hand pushing his shirt aside and gently encircled his manhood. It was already erect. He was afraid. He had never done this before."Do you think we should?" He asked looking down.Jolene nodded her head and looked down. Already Jim's teenage penis was pulsing at her soft, feather-like touch.Jolene's boyfriend, Tom, liked it when she held him like this, although she had only held him through his pants. Now she was touching Jim's naked penis. and apparently Jim liked it, too.Staring at her breasts, Jim hesitantly raised his hand, but he stopped short. "Are you sure it's all right?" he asked, unable to believe that he was being touched by the head cheerleader, the most beautiful and popular girl in class, and she was waiting, squeezing his penis ever so gently, her eyes watching his hand, her lips parted expectantly."I think so," she replied, nervously glancing around the room. "Go ahead. You can touch them." She whispered, her eyes again returning to Jim's hand.As gently as he could, he allowed his hand and palm to slide inside her blouse, beneath her breast as if to support it. He weighed it in his hand. She took a deep breath and her fingers surrounding his penis closed a little tighter. Jim closed his fingers around her breast, feeling its softness, and sensed her hold her breath. He wasn't sure what to expect, He had never touched a woman's bare breast before. Jolene's breasts were perfectly shaped, not too large, sagging ever so slightly under the effects of gravity ('would they sag a lot more when she grew older?' he wondered), with perfect nipples protruding slightly from tan aureoles located exactly where they should be. Her breasts were softer than Jim expected. Somehow he thought they would be firm and full, but decided that even though she was a senior in high school and had passed her 18th birthday, perhaps her body had not yet fully matured.His fingers found her nipple and gently grasped it, squeezing very slightly."Oh!" She gave a surprised moan and an unexplained tingle ran up her spine. Her fingers reflexively tightened around Jim's penis. He gently rolled the nipple, and she moaned again she gave a small whimpering sound, and again her grip tightened around his penis. She was holding him so tightly, it almost hurt, but he didn't want to say anything."Am I hurting you?" Jim asked, releasing her breast and lifting his hand slightly, barely maintaining contact with her breast."No!" she answered immediately, and dropping his manhood, her hand flashed to Jim's and she pressed his palm against her breast. "No, you're not hurting me. It's just;  I don't know,; "She hesitated."What is it?" Jim asked, sounding like he sincerely wanted to know what she was thinking."I've never felt this way before," Jolene stammered. "Do that again. I mean, with your fingers on my nipples."Jim took her nipple between his thumb and forefinger and squeezed."No; softer! They're very sensitive! Be gentle!"Jim rolled her nipples as gently as he could between his fingers and heard her moan, "Yes! That's it!"He kept rolling and her hand returned to his lap to find and grip his penis again."I don't know how to explain it," she whispered, her head leaning back against the sofa, her hand tightening around his penis."It; they're sensitive." She gave a small gasp, "and when you, uh, roll them like that, it stimulates them." (Jim could feel the nipples growing bigger and harder between his fingers.) Jolene continued, "and it; makes me feel; funny. It makes me feel funny in other places, too!" She was breathing faster now, and so was Jim.Jolene loved her boyfriend, Tom, and at his insistence, she had reluctantly let him touch her breasts. But he always mauled them like he was mixing meatloaf. had always been impatient and grew irritated if she asked him to be gentler or to do anything differently. Jim, on the other hand, was actually listening to her and doing what she asked him to. The difference was incredible. Although she was not in love with Jim, her body was reacting more strongly to him than it ever had with her boyfriend.Jim changed his hold of her breast, capturing her nipple between his index and middle finger, which freed up his thumb to push back her blouse giving him a better view of those beautifully shaped teen-aged mounds. He kissed her just above the collarbone, right where her blouse met her skin. She arched her back, pressing her breast into Jim's hand. Encouraged, he kissed her chest a little lower and again she seemed to press her chest into the kiss. Jim paused and looked up at her."May I kiss your;" She opened her eyes and met his. "Can I kiss;" Jim swallowed hard, trying to find the courage to speak the words."You can kiss my breasts. And my nipples, too. I think I'd like that. That is, if you want to!""I want to!" Jim replied, returning his attention toward her chest and gently brushing his lips against the flesh where the top of her breast met her chest. He changed his grip on her breast so once again, cradling her protrusion in the palm of his hand, but he still kept her nipple between his thumb and forefinger, gently rolling it. She squirmed a little, but made no attempt to pull away from him."It's funny," Jolene commented. "I always imagined sex as a nighttime thing. I never thought of having sex during the day."Jim nodded. "I guess you can do it anytime you wanted. I never thought about it." Somehow, Jolene doubted that he had really never thought about it.He kissed more of her breast and began moving his lips toward her nipple with each kiss. Shifting his hand to support her breast, he nibbled at the top of her nipple, then softly drew the nipple into his mouth.The electricity swept through Jolene "Oh," she moaned. He was gently, sweetly, sucking on her, pausing every now and then to kiss her nipple. It was exceedingly erotic and generated a ripple in her stomach.She looked down to watch Jim nursing at her teats and instinctively, she raised her hand to support her breast for his lips. As she took over the job of holding her breast steady, Jim's hand slid down her stomach, grazed over her skirt where it covered her pubis, and continued down the inside of her thigh until it came to the hem of her skirt and settled over the bare skin of her leg.Suddenly frightened again, Jolene pulled her breast back and grabbed Jim's hand on her bare leg. "Tom, don't!"Jim stopped his sucking and raised his head to look at her. "I'm not Tom," he whispered. "Tom is not here."Burning from mortification, Jolene quickly apologized. "I'm sorry, Jim! It's just that, well, I've had to stop Tom," Jolene paused and shuddered, "from doing what you're doing. I'm sorry.""I understand," Jim replied sympathetically.

[EP 25-076] James Woods tweeted: Two guys who have more money than they'll ever need are working for NO SALARY 16 hours a day to make the lives of hard-working Americans better. And all the Democrats do is bitch and moan. Former FBI assistant director Frank Figliuzzi just claimed that "If you voted for [Trump], you really need to question whether you're American anymore." This is a particularly chilling statement from a person appointed by Robert Mueller to head counterintelligence operations...I wish Elon Musk would use DOGE to find every anti-American and anti-Trump post on social media, so President Trump knows DIRECTLY who to fire.The Peter Principle, cooked up by Laurence J. Peter, is the brutal truth that in any hierarchy, people get promoted until they're bad at their jobs. You start off great, climb the ladder, and then—oops—you land in a role where you're completely out of your depth. Why? Because being good at one job doesn't mean you'll be good at the next one. It's like promoting a star quarterback to coach, only to realize he can't draw up a play to save his life. The idea was laid out in a 1969 book co-written by Peter and Raymond Hull. It was meant to be satire, but it hit a nerve because, let's face it, we've all worked for someone who proved the point. Now, it's a staple of management theory—and a perfect excuse for why your boss is the way they are. In short: promotions don't stop until incompetence does. Funny? Yes. Painfully accurate? Absolutely.Become a supporter of this podcast: https://www.spreaker.com/podcast/the-kevin-jackson-show--2896352/support.

Tiny microbes have a big impact on wine quality. Aria Hahn, CEO and co-founder of Koonkie, Inc., discusses the exciting work her bioinformatics organization is doing in the field of metagenomics. Hahn explains the differences between genetics, genomics, and metagenomics. She shares insights from a project studying yeast populations in British Columbia's Okanagan region, revealing the diversity and distinct clades found on wine grapes. The conversation also covers the broader applications of bioinformatics in agriculture, including regenerative farming, soil health, and potential bioprotectants against wine spoilage. Hahn underscores the impact of microbiome management on wine terroir and the potential of bioinformatics in understanding and improving winemaking processes. Resources:         201: Balance Hot Climate, High Sugar Wine with Green Grape Juice aka Verjus 243: Microbial Communities in the Grapevine 251: Vine SAP Analysis to Optimize Nutrition Aria Hahn – Google Scholar Aria Hahn – LinkedIn Koonkie Make Better Wines with Bioinformatics The Microcosmos - Discover the World of Genomics Apple App Whole genome sequencing of Canadian Saccharomyces cerevisiae strains isolated from spontaneous wine fermentations reveals a new Pacific West Coast Wine clade Vineyard Team Programs: Juan Nevarez Memorial Scholarship - Donate SIP Certified – Show your care for the people and planet   Sustainable Ag Expo – The premiere winegrowing event of the year Vineyard Team – Become a Member Get More Subscribe wherever you listen so you never miss an episode on the latest science and research with the Sustainable Winegrowing Podcast. Since 1994, Vineyard Team has been your resource for workshops and field demonstrations, research, and events dedicated to the stewardship of our natural resources. Learn more at www.vineyardteam.org.   Transcript [00:00:00] Beth Vukmanic: Tiny microbes have a big impact on wine quality. [00:00:09] Welcome to Sustainable Wine Growing with Vineyard Team, where we bring you the latest in science and research for the wine industry. I am Beth Vukmanic, Executive Director. [00:00:19] In today's podcast, Craig McMillan, Critical Resource Manager at Niner Wine Estates, with longtime SIP certified vineyard and the first ever SIP certified winery, speaks with Aria Hahn, CEO and co founder of Koonkie Inc. [00:00:35] She discusses the exciting work her bioinformatics organization is doing in the field of metagenomics. Hahn explains the differences between genetics, genomics, and metagenomics. [00:00:47] She shares insights from a project studying yeast populations in one of British Columbia's wine growing regions, revealing the diversity and distinct clades found on wine grapes. [00:00:58] The conversation also covers the broader applications. bioinformatics in agriculture, including regenerative farming, soil health, and potential bioprotectants against wine spoilage. [00:01:09] Hahn underscores the impact of microbiome management on wine terroir and the potential for bioinformatics in understanding and improving the winemaking process. [00:01:19] We know your customers are looking for sustainable wines. In a recent review of 30 studies, Customers reported a higher preference for eco label and social responsibility labels compared with nutrition labels. Achieving SIP certified gives you third party verification that your vineyard winery or wine has adopted and implemented stringent sustainable standards. Apply today at SIP certified. org. [00:01:46] Now let's listen [00:01:50] Craig Macmillan: Our guest today is Aria Hahn. She is the CEO and co founder of Koonkie, Inc., a bioinformatics organization, business, doing all kinds of exciting stuff. Thanks for being on the podcast, Aria. [00:02:02] Aria Hahn: Yeah, so excited to be here. Thanks for [00:02:04] Craig Macmillan: We're going to get into the thick of it But we were attracted to some work that you folks and your colleagues have done with bioinformatics and yeast, wild yeast. But I wanted to drop back. A little bit first to kind of give some context. All of this kind of comes under the umbrella of metagenomics, correct? [00:02:21] Aria Hahn: Yeah, absolutely. [00:02:22] Craig Macmillan: and what is metagenomics? [00:02:24] Aria Hahn: I'm going to take further step back and talk about genomics um, maybe the distinction between genomics and genetics. [00:02:32] So genetics is something I think most people kind of understand. They have this intuitive sense of it. um, that make up ourselves and all living creatures. But it actually turns out that in most organisms, and not bacteria, and we'll get there in a Most of your DNA is not in genes. It's in between genes. And so genomics is the study of genes and all of the things that are in between genes. So that's kind of the distinction between genetics and genomics. And then metagenomics is when we do that at the community level. [00:03:02] so you could do metagenomics of humans, but metagenomics refers to populations of bacteria, uh, microbes, archaea, viruses, things that you cannot see and I'll say interact with directly. [00:03:17] Craig Macmillan: And then bioinformatics is a subset or is a technique, is that correct? [00:03:23] Aria Hahn: Yeah, it's a technique, you know, it used to be even 10, 15 years ago that everyone kind of did their own bioinformatics. And so really what that means is when we sequence DNA or read that DNA, so it's only four letters, ATCs and Gs, we extract the DNA the sample is, could be the surface of grapes, uh, a human, anything. [00:03:42] Um, Then we put it on a sequencer. There's a bunch of different sequencing technologies right now. Um, But you end up with these like very gross files that aren't openable on regular computers and they're literally just ATCs and Gs. And so bioinformatics is the cross section of high performance computing and biology. And so we develop algorithms and processes and pipelines to really take those gross huge files of ATCs and Gs and make them human readable. make them interesting, figure out, you know, what are the genes that are there? Who is there? What are they doing? And who's doing what? [00:04:19] Craig Macmillan: Okay, and I think that's the important part here is you would take a sample from the environment. We'll talk about this one more in a second, but there are particular sequences that may be associated with a certain type of microbe or even a particular genus or particular species of microbe that can be detected. [00:04:39] Aria Hahn: Yes, absolutely. [00:04:40] So a genome is all of the DNA that makes up that organism. So you and I have distinct genomes, but of course, our genomes are going to be pretty similar to each other compared to a human genome, to a fish, to a plant, to a [00:04:55] Craig Macmillan: why the focus on microbes? [00:04:57] Aria Hahn: Yeah, that's a great question. It depends how philosophical You want to get You know, people are generally [00:05:02] familiar with the concept of like the Higgs boson particle. It's like the God particle that makes up everything and that's great and the physicists are gonna come for me. But when we think about our planet Earth, I always say like If there is a god particle on this planet that is alive and that we interact with, like, it's a microbe. [00:05:21] This is their planet. They were here long before us and they will surely be here long after us. So we think about microbes in terms of they are the destroyers of higher level populations. They keep us healthy. They make us I'm going to say it's a great example, but it wasn't a great thing. [00:05:40] So caveat that. But COVID was a great example about how this is not our planet where we had an of a virus in one location in a very particular place in the world. And all of a sudden it was across the planet. We are vectors for them. [00:05:58] You know, looking at those maps and showing the spread and how quickly it happened, I like to use that often in visual presentations to say, if you don't believe me, like, look at this. It's spread through us. [00:06:10] Craig Macmillan: Right. And I think also this gets to some other things we might talk about later on, but there are communities of microbes that are associated with certain macroorganisms. [00:06:23] Aria Hahn: uh, so are, they're everywhere, they're on your um, there's lots of research in the cosmetic industry that's looking at that. There was this crazy CEO years ago where he was I'm gonna slather this microbial laden cream on my skin and then I'm never gonna shower again and I'm not gonna smell. Not necessarily like my cup of tea, I love a good hot shower. But, you know, it can be there. The soil is the microbial diverse environment on the planet. , But your gut, like you, you as a human being, can't actually digest your food without those microbes. You can't get those vitamins and nutrients that you need without that community in your stomach. [00:07:03] Plants work the same We say charismatic macrofauna, eagles, whales, things that are very Um, They, they don't to, you the seaweed and the weeds and the grass and kind of everything in between. it's All supported by the microbiome, by these microbial communities. [00:07:20] Craig Macmillan: so let's talk about one microbiome in particular, and that would be populations of yeast that we find on wine grapes. [00:07:29] Aria Hahn: Yeah, yeah. So we've looked at yeast and bacteria and they're both cool. [00:07:34] Craig Macmillan: That is super cool. And so this one particular project where you looked at yeast on wine grapes in British Columbia Tell us a little bit about that project [00:07:41] Aria Hahn: there's, So I live in I'm, I'm right in the thick of, you know, BC wine country, which is a fantastic place to live, we were fortunate enough to work with the Wine Research Institute out of the University of British Columbia, Vivian Mease Day's group. them and They do very, very cool work, but they were trying to look at the yeast populations in wineries across the Okanagan region. [00:08:02] We know that the history of lots of commercial. Yeasts are actually from oak trees in Europe. So that's very cool. And what we wanted to see is how are the yeasts that are being used to produce wine in the Okanagan region distinct and similar to commercial yeasts and yeasts that have been characterized from across and so We did just that and we were actually able to sequence a whole bunch of yeast. And so, again, that's like reading the genome effectively there. so we found four distinct clades, um, in the Okanagan region. And a clade is they're related groups, and so it's not like you and you're a twin where you might have an identical, uh, genome to a twin. [00:08:50] It's more like you and your cousins and second cousins and second cousins twice removed and, you're, you're kind of vaguely related to each other. You kind of cluster over here, but you're not necessarily super We've kind of found four clades that the 75 yeast strains that we studied in that particular piece of work Really related to, then we looked at like what is different in their genomes. [00:09:12] So they're all the same species. That's the first thing to, to think about here. So just like you and I are the same species, they're all the same species, but just like you and I, we have different areas of, of specialties. Some people podcasters. Some people are, artists and scientists and, um, kind of everything in between. [00:09:33] And we need everybody. So, we're all the same species, but we have different specialties. And the yeasts work very similarly to that. [00:09:40] Craig Macmillan: all right, so this is interesting to me so You go out and you you said when you looked at 75 species of yeast or different types of yeast Those are ones that you, you found. It wasn't like you went in and said, I want to test for each of these 75. You got information, you got data in and said, Oh, look, here's 75 different types of [00:10:01] organism. Yes, that's a, that's a great Um, so, we And we uh, the ferment or the, the yeast skins and we extract the DNA and then we get rid of the great DNA, which could probably also be really cool, but we didn't look at it in this case. And kind of threw that into the and then said, okay, we're just going to focus on the Saccharomyces cerevisiae Latin term for a very common yeast strain, um, used in wine. And we said, we're going to look for it. [00:10:30] Aria Hahn: Then we found actually hundreds and . And then, um, and I didn't do this work, I don't do a lot of lab work myself, so, uh, this part is kind of the edge of my knowledge. But there is some ways to kind of do microsatellite clustering. And so you look, and you look for tiny differences in the genome, and you say, okay, maybe we found 500, but we actually want to look at ones that are distinct from one another. So we don't want to randomly pick 75, we want to pick 75. strains of this yeast that are different from one another. [00:11:01] And so you could use some lab techniques to make that happen. And then you take those hundreds and we say, these are the 75 that we know are different. We're going to dive deep into those 75 so that we can kind of get this breadth of genetic diversity from the region. [00:11:18] Craig Macmillan: And that was something I was thinking about. You mentioned you took samples from either fermenting wine or recently fermented wine or from skin material. How exactly is this collected so that you know that you're getting just [00:11:30] what you want? [00:11:31] Aria Hahn: Yeah. Painfully is the answer. So like when you do soil sampling, it's actually really And we tell people all the time sampling for yeast or microbes is not that complicated. I say every single time we talk to a client, I'm like, look, wash your hands with ethanol, you know, hand sanitizer, essentially between rinse your tools. And mainly you can't mess this up as long as you don't spit in the bag or bleed in the And I say this every time, and I will say one out of every few hundred samples is full of blood. Hands down like you always think we always the that and then hands down. We're like, this is full of And I think it's just like a matter of working in the field like people nick themselves They don't really realize that but really that kind of thing is really easy [00:12:15] When it comes to sampling a ferment that can also be fairly easy. [00:12:19] You have a lot of it You can kind and put it in a jar, but I will Um, Jay Marknack, who's done a lot of this work and developed a lot of these methods, he actually developed this method that is painstaking. Um, But you have like, he's got this method where he takes the grapes really carefully without touching them off of the and then washes just like very carefully with these like rinse solutions to really just get the microbes and yeast that are on that surface without touching it, without touching other surfaces. It's really just what was there in the field. And rinse all of that off. And you can imagine that's not that fun of a Um, And, and, and so, and it wasn't like he did it on his first try either. So he's now developed that we're copying and using, thank goodness. Uh, But it can be like that kind of painful where it's like washing individual grapes, collecting that rinse water, and then filtering that rinse water, like onto a physical filter, then extracting the DNA from that filter. [00:13:18] It's not fast. [00:13:19] Craig Macmillan: Nope, that's what I wanted to know. I've collected a lot of soil samples in my life for looking at soil microbiome. And you know, technique is everything. You know, contamination will mess you up pretty badly. [00:13:29] Aria Hahn: We had this one study I felt so bad, but they had collected these samples. They sent us the samples and we get the data back and it's, they're soil, they should be teeming with life, right? [00:13:38] And there's like one species basically in this thing, like there's a handful, but like one is dominating. So we go to them and we're like, what is going on here? And they're like, well, I don't see how that could have happened. , we've been storing these in a dark closet for a year. And we're like, that's why. You are studying bottle effects right here. And they're like, oh, we thought it'd be fine because it was dark and cool. And we're like, yeah, but it's not open to the air, and it doesn't have the plants and animals and bugs. You grew one guy. [00:14:07] Craig Macmillan: Yeah. We've been talking about bacteria, or the yeast. Are there other types of organisms, microorganisms, that you can use this technique with? [00:14:14] Aria Hahn: Totally. So you can use this technique on basically anything that's alive. So you could target viruses, uh, not something we've done on wineries, but could absolutely do it. You can target, , archaea, which are very similar to bacteria in that there are a single cell. But they are similar to eukaryotes. [00:14:32] So things that are bigger, um, like us, like mammals, like fish, Uh, but they are kind of small and invisible, , to the naked eye like bacteria. So those, we can, we can do that all the way up to, any animal that we can see, feel, touch, , and kind of anything in between. So it's a really powerful technique. As long as it has DNA, we can make this work. [00:14:53] Craig Macmillan: So you found these 75 types? of yeast organisms, but they fell into groups, they fell into clades. And I thought that was one of the most interesting things about this. Can you tell us a little bit about the natural history of behind these clades and kind of what that means? [00:15:09] Aria Hahn: We found these 75 different strains and they did group into four clades. So four kind of groups of more or less related organisms. So you can think of them as like clustering based on similarity. [00:15:22] The first one was one that is well known and well studied. So that's wine and European. And so those strains are more similar to these that we see in wines out of Europe and commercial strains. [00:15:35] And then the second clade we saw was the trans pacific oak. So a lot of wine yeast are very closely related to yeast that are found on oak trees. And so actually think that, , the original, , European wine yeast strains from, you know, the 1800s are from Mediterranean oak trees. And so it's not uncommon that we see these strains related to oak. [00:15:59] So that was the trans pacific oak. Then we see another group or clade that we called beer one mixed origin. And so we saw similarities to known previously studied yeast strains that are related to beer, sake, so other kind of fermented drinks. also kind of expected. [00:16:18] And then what was really exciting is that we found a new clade that we've designated the Pacific West Coast wine clade. it's always neat when you get to discover something new, of course. And so it has high nucleotide diversity. And so what that means is that even within this clade we do see a lot of genetic diversity kind of in there. [00:16:38] And what we do know is that that whole clade shares a lot of characteristics with wild North American oak strains, but, and this is kind of where like it all kind of comes full circle, but we also see that it has gene flow from the wine European and Ecuadorian clades. It can mean a couple of things. So it could mean that There is just so much selective pressure when you're, when you're trying to make good wine that these genes that are found in European wine strains, commercial wine strains, they're present in Saccharomyces cerevisiae in general, but then when we try to make good wine, we select for strains that have these, genes, , that we know produce good wine, because they produce good wine everywhere. [00:17:27] And so it could just be this process of natural selection. It also could be that most wineries , are not purists. It's not that. never in their history have other wine strains visited their their vineyards. They might have tried a commercial strain. They have wine from others, you know, people track things in, animals track things in. And so it could just be that there is this gene flow, quite literally from, from Europe, from these wine strains that just kind of comes into our population here in the Pacific West Coast. [00:18:00] And so there's kind of these, these two ways that we could have got these things, We do have some evidence to suggest that they were actually transferred in. [00:18:07] So it's called horizontal gene transfer. And my go to example on how horizontal gene transfer works is always , The Matrix, like the movie with Keanu Reeves. But what I've also learned is that if you talk to people that are like younger than me, they don't know that movie anymore, so this only lands with like a certain age of [00:18:23] Craig Macmillan: Right, I know, I know, [00:18:24] Aria Hahn: You know The Matrix where they like plug in and then they have all these new skills? [00:18:28] Bacteria can kind of do that, where you can just take genes from, , a relative, has to be like kind of closely related, and we take them and then we just put it into their genome, and in many cases, not all, but many, they're able to just kind of start making use of those genes right away. [00:18:43] And so that's horizontal gene transfer, which is pretty cool, because for us, the second that sperm hits the egg, that's it. That's all your genes. You're not getting more. You're not losing more. Like you're, you're set. But bacteria are more fluid. [00:18:57] So there is this cool thing called the wine circle, and it's a cluster of five genes that are associated with making commercial wine. [00:19:05] And we do think because we see this wine circle and these particular five genes in so many wine strains, and because of their location and a whole bunch of other kind of genomic characteristics of them, Um, we think that they are horizontally transferred. And so we do see this wine circle of these five genes in the majority of this new clade of British Columbia strains. [00:19:33] Craig Macmillan: So just talking about moving things around the world, you said like people have things on their bodies and whatnot. I, I was fascinated by the Ecuadorian group. And is that literally like it was growing on plants in Ecuador, kind of native to that area that is found its way up the West coast of North America. [00:19:53] Aria Hahn: that's really what we thought happened. I know it is amazing, right? Like does the amount and transfer and you know how you go through the airport and they're like, you and It's like the end of the world. It's like I get it because we don't want to like do that on purpose, but also the ecuadorian yeast like it's coming up here [00:20:12] Craig Macmillan: right, right. Exactly. [00:20:14] So what I think is of interest to winemakers, and also has potential beyond that that I'll ask you about winemakers are looking for increased complexity in their wines, and they're also looking for a sense of place. And I'm really happy to hear more and more people talking about terroir, not just in terms of rocks, but in terms of the whole picture. [00:20:33] The soil microbiome, the practices that are done, as well as light and climate and all those kinds of things. What are some of the things about what you found that indicate or that suggest a uniqueness to that Okanagan area that may make it stand out as different than other locations? How does this translate into sense of place? [00:20:54] Aria Hahn: That's a fantastic question. I'm going to give two answers first on the east side. We see that many of that nucleate. don't have all five of those wine circle genes. And so we see a lot of British Columbian strains have that, but there's this whole clade of these natural yeast used in wine that don't have all five of those. [00:21:17] So then you just have different genes to work with. And since you have different genes to work with, it's not just those genes, but it's all of the genes, and it's the rate that those specific strains are able to break things down. [00:21:28] You do get this added complexity when you're not using a standard commercial yeast. You just have this bigger variety of genes to choose from, and That's going to make the flavors more specific, and different. [00:21:44] It also introduces a certain, the disadvantage of using these is that, you know, they are gonna vary year to year, month to month. Uh, Potentially, and, and so you might get really, really amazing results one year and not the next year, and understanding why, why that might be is a whole exercise in and of itself, probably doable, but it's really exciting to think that these yeasts that are there naturally , they just have that genetic diversity and they want to live in these diverse communities, and so you are going to get that difference and terroir. [00:22:16] The other piece that was really exciting and was a different piece of work, but very similar groups and very similar, , samples, was looking at the microbiome, so the bacteria on the grapes. And we kind of found two things, and so there is some literature that shows if you look at a single farm, a single vineyard, and you look at different red varietals of grapes, you see actually a fairly similar microbiome signature on all of the different varietals. [00:22:46] Okay, but if we look at three distinct vineyards that are all within , one kilometer radius of one another. So they're very close. They have the same rock, to your point. They have the same weather. They have the same climate chaos happening, [00:23:01] but they're managed differently. We actually see very, very distinct signatures on all three that persists year after year. So we looked at two years, , this was again, Jay Martinek's work, , and we see that each one of those, even though it's the same varietal of grape, it is more similar to itself, year over year, than among the three farms. and and that's very interesting because what that suggests is Exactly what you're saying. [00:23:29] It's not the rock. It's not the climate that's driving the microbiome there. It's actually the practices of that vineyard that are changing that. And to me, that's so powerful, because what that means is that there's so much of that craft and art in the management of the vineyard that's then going to go and affect the terroir. , I know that's not the yeast answer, but that's the bacteria answer, and it's like, the power's in your hands. [00:23:54] Craig Macmillan: I'm on the Central Coast of California, and we've had some very hot vintages in this last , 2024 season. We had, and it was 2022 as well, we had these really hot stretches of over 100 Fahrenheit. Not very friendly to yeast in general. [00:24:09] Probably friendly to some, but not to others. And I had conversations with winemakers along the lines of like, could you even do a natural fermentation this year, a native fermentation? Are they there anymore? Or have they been selected against due to the heat? And I now have a total reset of the microbiome, the microflora in my world. this is the kind of thing that bioinformatics would be able to determine. [00:24:34] Aria Hahn: yeah, for sure. So we love that. We love when we get the baseline. We're like, show us your year that you were like, this is my typical year. This is my regular year. We'll live for that because as soon as you have the baseline, then we can go and answer those questions. So we can say, okay, great. We know what your baseline is when you typical year. [00:24:52] Now you have this heat wave that comes in. , Let's go and look. Let's go see who's survived. And I know I anthropomorphize all of these things a ton, but it really is, like, who's there, right? , is it the same bug, but very decreased? Are we getting different E strains coming in? are we seeing less overall diversity? [00:25:13] Do we see the same diversity, but Their population is a quarter of the size, and how does that affect the dynamics? Like, what do we see? And bioinformatics can absolutely absolutely answer these questions. And that can be really powerful. [00:25:26] Craig Macmillan: In my research I didn't pick up on this Can bioinformatics put a quantity on things? Can you quantify the relative size of these different populations? [00:25:34] Aria Hahn: We can, yes. So, you have to use some kind of special techniques. There's a couple of main ways we do them. One is called qPCR, so quantitative PCR. And so we literally take the DNA and we can count the copies of it in a very quantitative way. That's straightforward, pretty inexpensive. [00:25:52] Another way we can do it is a little bit more sophisticated, , but you don't have to know what you're looking for. So with quantitative PCR, we have to know, like, we want to go count saccharomyces cerevisiae. But if we don't know all of the microbes that are there, all of these that are there, then we can't go and target it with qPCR. So then what we have to do is use a spiken. the concept is pretty simple. You put a known quantity of a piece of DNA that we would not expect to appear in nature. And then when we sequence it, we know how many we got back. So if we know we put in a hundred copies of it and we get 200 copies back, now we have a pretty good idea of like, everything there was, sequenced twice or if we get 50 copies back, we're like, okay, well, however many we have, we're going to double that and we have a good idea and we do do this in like a little bit more sophisticated way where we put in like a whole bunch at different quantities so we can double check our math and make sure that it's all good. [00:26:49] But that's the concept is with a spike in so you can do it quantitatively. [00:26:53] Craig Macmillan: Talking about all the things that are out there, there's a lot of interest right now in bioprotectants for fermentation, where you introduce non fermentative yeast, and they kind of take up the ecological niche against foliage organisms, and then you can add a Cerevisiae strain to do that, to do your fermentation. [00:27:10] Would you be able to pick up those other genus, of yeast in a bioinformatic way and gives us a sense of what else is out there. [00:27:18] Aria Hahn: Yeah, for sure. So we sequence the whole community and then we kind of in a. Like a puzzle. I'm going to put together the individual genomes of everyone who's there. And so we can look at not just the targets, but the unknowns as well. And so often, especially in soils, what we get is sometimes up to 80 percent of the genomes that we're able to recover from that sample are totally novel. [00:27:43] So they're new to science. It's really exciting. and we hate it. We love it and we hate it. So, we love it because it's really fun. You, you discover these new species of bacteria, of yeast, or these new strains, and, and you get to name them. You don't have to name them after yourself anymore, you have to name them about the place that they're there. Which is a totally logical thing. But, would have been fun. , [00:28:06] So we get to name these things, it's really exciting. [00:28:09] But it also means it's so much work. Because now you have this genome that's so new. And so now you're trying to figure out. What are all the genes? Do we know the genes it has, but just not quite the way that they're arranged? Do we not know what many of these genes do? And if we don't know what these genes do, like what kind of uncertainty and questions does that bring up? And so it can be really exciting, that discovery phase, and also quite overwhelming, honestly. [00:28:36] Craig Macmillan: what other applications might there be for bioinformatics in wineries or in vineyards? [00:28:41] Aria Hahn: Yeah, that's fantastic. So definitely monitoring. You know, regenerative farming is a really big thing right now. how can we introduce additional species, cover crops, , you know, planting additional or different plants in between. Like, what can we do to really increase the soil health, sequester carbon, the biodiversity of the soil, of the land, and how does that affect it? So we can monitor all those things with environmental DNA or eDNA. [00:29:09] One thing that we've been thinking about a lot is this concept of smoke taint, which I think has kind of affected the whole west coast of North America. [00:29:18] Are there microbial treatments that can kind of mitigate smoke taint, , can we feed bacteria, the bacteria that we know [00:29:29] can kind of break down those volatile phenols that cause the smoke taint. Get them to kind of break that down first before we make the wine. Like we're kind of looking at applications like that. [00:29:40] Obviously those are, I would say further out in terms of technological development biodiversity, which we can absolutely go and do today. , but there's interest in that smoke taint. Application, and we're really interested in that. [00:29:52] Then there's also kind of everything in between. So can we the harvest? Can we increase the quality of the grape? Can we help with years that are dry? Can we help with years that are wet or cold or hot? as we, kind of committed to a certain number of effects of climate change, we have to start thinking creatively. [00:30:14] I was on this call with an unrelated company. They wanted to do similar things but in the mining space, in the reclamation space. And I don't know how it happened, but I was on this call with this man. It was his last day before it was dark where he was. He's in Quebec. He's three hours ahead. [00:30:29] , You know, it was winter. So it's very, the mood was very, like, dark and somber, and this was his final call of his final day of work. And he was so hopeful about microbes, and he spent his career working with them. And just before he signed off the call, he says, I hope microbes save us all. [00:30:50] And then he kills the call. And, and, for, the next few years, I titled every single talk I did, Microbes may save us all because I just, the weight of that conversation was so big and I know that's not what we're talking about here in terms of [00:31:08] smoke taint, but I do think, you know, to bring us full circle to this like omnipresent godlike presence of microbes that there's something to that idea in that I think that they have this potential to save us from ourselves. If we can learn more, [00:31:25] Craig Macmillan: I think what we're talking about is bioremediation and the potential there. And bioremediation would work by identifying an organism that's going to play a certain role and then actually introducing it into the environment. For instance, like introducing it to wine that may have smoke taint, for instance. [00:31:40] Aria Hahn: , so there's a three main approaches to that. [00:31:42] So the first is exactly what we're talking about. You introduce a micro that we know and you, and you put it in there. The main challenge of that is this, this word we call engraftment. We actually steal that word from organ transplants. So, when you put in a new heart, not that I know anything about heart transplants, but when you put in a new heart, you have to engraft it. [00:32:01] And so people need to be on immunosuppressants, is my understanding, to make that heart transplant like stick in their body, have their body accept it. Kind of the same challenge when you introduce a microbe into an open, wild environment where you need that new species to engraft in that community. If you can't do that, you just have to keep adding it. [00:32:21] You have to keep adding it, keep adding it, it's time consuming, it's expensive, all of these things. So engraffing is still a challenge in that field. But that is one way. [00:32:29] The second way is to bioengineer. And so the concept here is that you take species or strains that are naturally occurring, so they do well in that environment, and you change something in their genetics and then reintroduce that. It does get around the concept of, [00:32:50] of engraftment in theory. The major issue with it is, there's not a lot of people or companies that feel ready, I think, to take a biologically engineered synthetic genome and introduce it into the environment en masse. We just don't understand the risks of it, or, or not, we don't know, but I think that's the point, is that we don't know, and so people are a little bit like, Maybe we're not quite there yet. [00:33:19] And then the third way is to say, I'm going to look at who's already there. And I'm going to understand what they like to eat and what their competitors like to eat and I'm going to try to starve their competitors [00:33:31] and really feed the ones who have the capacity to degrade those volatile phenols. I'm going to like try to get their population to do super well and thrive. and and try to kind of starve out and make the populations that can't do the job that I want lesser and less prevalent in the community. [00:33:51] And that approach I think is kind of one of my favorites where we understand and then we put some selective pressures. So this could be adding more nitrogen, adding different carbon sources. [00:34:01] It could be watering less to create a more aerobic environment. It could be you know, kind of drowning them to create an anaerobic environment. It's kind of those bigger controls that we have working with the microbes that are already there. [00:34:17] Craig Macmillan: Yeah, in the same way that we're not afraid to play with plant communities in agricultural systems, with cover cropping or intercropping or anything like that, same kind of idea, where maybe I plant something that I think will out compete a weed. [00:34:28] Same kind of idea. And we're pretty comfortable with that. [00:34:32] And also things will have a way of finding their stasis, finding their, their It's just getting it kind of pushed in the right direction. I think that he's super, super cool. [00:34:44] A lot of interest and work in the soil microbiome in terms of soil health. We mentioned regenerative agriculture. I have put my toe in that, in, in my professional world extremely difficult, extremely confusing, lots of holes you know, and, and trying to find markers or metrics has been. challenging for instance, I was trying to figure out how healthy some soils were. It, healthy in quotes, and I wanted to do analysis of respiration. And this very good soil ecologist said, well, that tells you how many folks are in the room, but it doesn't tell you what they're doing or who they are. [00:35:21] And I was like, that's a really good Point I could have a lot of respiration from organisms. I don't want and I wouldn't know what was who and who was what? What world can bioinformatics play in that [00:35:33] Aria Hahn: , that's a great question. So I would say it's the opposite in general, without the spike ins and kind of specific things, what? we can tell pretty inexpensively, 50 to 100 a sample, is basically who's in the room and in what relative abundance. So it is come down a lot in price. It doesn't tell us a lot about their genetic capability. [00:35:55] So if we know them because they've been previously studied, then we can say like, oh, yeah, these guys are known to do X, Y, and Z. [00:36:02] If we don't know them, for that kind of price point in those methods, we're kind of just like, yeah, we know their names. But that's it. [00:36:08] Then we can do kind of a deeper dive, , to a different type of sequencing called whole genome sequencing. And you get the whole genome. And so there we can actually say not only who they are, but what they're doing. Or what they have the ability to do. And so that's where the limit of DNA is, is that it can tell us the potential. They can potentially do this, but it doesn't actually tell us if they're choosing to do that, so to speak. [00:36:33] There are other techniques that are very related. Metatranscriptomics, it's looking at the RNA, and you could do metabolomics. So you can actually look at the metabolites that they're producing, and then it tells you what they actually did. But we often can start at that base layer of DNA. and build up. So those questions we can answer. [00:36:51] And I think you're right about there are a lot of holes and it's confusing and it's complex. And we say this to clients all the time, like, if you know way to solve a problem, do that. Biology is messy. [00:37:03] But if you don't, like let's look at biology and let's enjoy the mess , there's a lot of beauty in that mess. And that's one of the things we've actually loved about interacting with wineries they are incredibly scientifically minded folks. They're data driven, the amount of innovation and technology they're using. never fails to impress, but you also get that love of the art and the craft from them. We love that. We see art and science as like in a circular spectrum. And so we love when, our clients in the, in the wine start talking to us about kind of their secret sauce and the things that they've tried and how, and they always get a little bit nervous. [00:37:49] And they would, if they always kind of start, they were like, you know what else I do? And we're like, tell us. And then they tell us something and they're like, we just know from experience. Experience that this works that this changes the ferment, but we don't have any evidence for that And and I think they're worried we're gonna judge them but we're like no that is like their science is all way of knowing but [00:38:09] my friend says art is science and love and and I love that idea that is something that's been really really fun about working with wineries and vineyards is they kind of get that they're like, yeah, this is the love piece here [00:38:22] Craig Macmillan: That's cool I think there's beauty in the mess. I might adopt that if you don't mind I mean, I may use that for some of my own stuff. I think that's great What is one thing you would tell growers or wineries, , [00:38:35] Aria Hahn: their choices are directly impacting the microbiome, so that's the bacteria and the yeast And that that is going to affect the terroir, the complexity, the quality of the wine, and it is knowable. [00:38:50] Craig Macmillan: there we go. And we also know that some of the things that we do may affect that and that is part of what makes us special. Where can people find out more about you? [00:38:58] Aria Hahn: We have a website, it is koonke. com, K O O N K I E dot com. can also look me up, Aria Hahn, , and on Google Scholar, the internet, I feel like I'm very findable. [00:39:10] Craig Macmillan: Yeah, you are very findable and we will have a lot of links and other things on the show page. So please check that out. Really fascinating stuff going even beyond this. I want to thank you for being on the podcast. [00:39:21] This has been a great conversation. [00:39:22] Aria Hahn: Yeah, thanks for having me. Super fun. [00:39:25] Craig Macmillan: So our guest today was Aria Hahn. She is CEO and co founder of Koonkie, a bioinformatics company, and is doing some really fascinating stuff, not only around yeast, but lots of other topics. [00:39:35] And I just got lost down the rabbit hole when I took a look at that website, all the different things you folks have been involved in, and it was really fun. [00:39:48] Beth Vukmanic: Thank you for listening. [00:39:49] Today's podcast was brought to you by Sunridge. For over 45 years, Sunridge nurseries has supplied premium quality grapevines. to grape growers worldwide. A pioneer in the industry with a focus on clean quality vines and personalized dedication to their partnered growers has led them to be the largest, most well respected grapevine nursery in the United States. Sunridge Nurseries continues to lead the industry having undergone several expansions to their modern state of the art facilities and is the first and only grapevine nursery to have implemented the most advanced greenhouse Horticulture water treatment technology in North America. [00:40:26] Make sure you check out the show notes for links to Aria, an article titled, make better wines with bioinformatics plus sustainable wine growing podcast episodes, 201 balance hot climate, high sugar wine with green grape juice, 243 microbial communities in the grapevine. And 251 vine sap analysis to optimize nutrition. [00:40:50] If you liked the show, do us a big favor by sharing it with a friend, subscribing and leaving us a review. You can find all of the podcasts at vineyardteam.org/podcast, and you can reach us at podcast at vineyardteam. org until next time, this is sustainable wine growing with the vineyard team.   Nearly perfect transcription by Descript

Find out more about Rep. Josey Garcia here.  all the podcasts.our FB pageour website.our Youtube channel.our patreon.our Twitter.our Insta.Our TikTok.Our Reddit.Our newsletter signupCome see our live show! October 11-13th, 2024 at the Scottish Rite Theater in Austin, Texas. .

We revisit the movie where Rachel McAdams and Jason Bateman are the comedy duo of the century. Zach, Kelsey, and Garrick lust over the witty jokes and brilliant performances. Learn more about your ad choices. Visit megaphone.fm/adchoices

Guest: Vice-Admiral Mark Norman, former Commander of the Royal Canadian Navy and former Vice-Chief of the Defence Staff. Learn more about your ad choices. Visit megaphone.fm/adchoices

Join me with my guest Ryan Tuckwood, Australia's top sales coach, who highlights the importance of skill development in sales and job interviews, emphasizing that being yourself isn't enough to reach your goals. Learn more about cold calling, building relationships, and personal branding in today's digital age. Don't miss out on Ryan's powerful reminder to take risks and roll the dice—sometimes, that's exactly what you need to unlock your full potential. Episode Highlights: 00:00 - Episode trailer 02:33 - Client Success Stories 05:03 - Overcoming Introversion 07:18 - The 10-step Negotiation Ladder 10:04 - Making a Strong First Impression 12:53 - How to Shift Emotions Positively in Interviews 19:15 - Understanding Personality Types 27:39 - Decision-Making Strategies 30:44 - Barack Obama's 60% Rule 33:38 - Understanding Worst-Case Scenarios 36:27 - How Skills Build True Confidence 39:39 - Crafting the Perfect Cold Call 45:36 - Exploring the Psychology of Authority 52:23 - Using Subtle Language for Authority 57:08 - The Power of Preempting Scripts 01:05:17 - The Two Words You Don't Want on Your Deathbed About my guest: Ryan Tuckwood is a renowned sales coach and the co-founder of SWISH Sales Coaching, widely recognized as the No. 1 ethical sales coach globally. Once a struggling, reluctant sales professional with just 31 cents to his name, Ryan turned his life around through relentless study and a deep understanding of sales as a process. He has since coached nearly 6,000 businesses and individuals worldwide, helping them generate over $1 billion in new sales revenue. Ryan's clients include top companies like Mercedes-Benz, AMEX, and Westpac. His expertise has earned him the opportunity to share the stage with figures such as Grant Cardone and Jordan Belfort. In 2018, Ryan and his co-founder Jack Corbett made history on Channel Ten's Shark Tank, securing the support of three Australian entrepreneurial heavyweights—Dr. Glen Richards, Steve Baxter, and Andrew Banks—who are now shareholders and advisors to SWISH Sales Coaching. Connect with Ryan Website: https://www.swishsalescoaching.com/ Instagram: https://www.instagram.com/ryantuckwoodofficial/ YouTube: https://www.youtube.com/channel/UCL93CDfMKRqglmU_8ey_YbA Become a globally recognized Trainer and Speaker in just 90 days! https://www.speaktoleadblueprint.com/speaktoleadblueprint Want to manage your life the same way that helped me make $1 million by 28 and travel the world at the same time? Grab my FREE one-page Life By Design productivity planner below ⁠https://planner.morgantnelson.com/optinplanner Join the Dream Out Loud Facebook Community https://bit.ly/49QXClW Follow me on Instagram here https://www.Instagram.com/ Subscribe to my YouTube channel https://www.youtube.com/@morgantnelson

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“Family Proclamations” is the current podcast by Blair Hodges, host of Fireside. Enjoy this sample episode and be sure to subscribe directly to Family Proclamations now, because this episode will fall out of the Fireside feed next month!

Paul Scheer is an award-winning actor, screenwriter, comedian and podcast host of How Did This Get Made? So you'd think his most interesting life stories would be about his audition for Saturday Night Live or how he came up through improv comedy to star in shows like The League and Black Monday. Those are interesting stories, but in his new memoir, Paul gives center stage to family stories. Growing up with a loving mom and dad who divorced, the tyranny of an abusive stepfather, and what it's like to become a marriage partner and a parent in the shadow of traumatic experiences.  About the Guest Paul Scheer is author of the New York Times best-selling memoir, Joyful Recollections of Trauma. He's a comedian and a Screen Actors Guild Award-winning actor from shows like The League, Veep, Fresh Off the Boat, and Black Monday. He's a podcaster of the smash-hit How Did This Get Made and also Unspooled with his co-host Amy Nicholson. He lives in LA with his partner June Diane Raphael and their two kids.  Full transcript available at familyproclamations.org. 

In this episode, transformational coach Emile shares the hidden challenges faced by high achievers, sharing his own journey from the modeling world to realizing the importance of self-acceptance. Discover how the quest for external validation can lead to burnout and offers practical strategies for fulfillment, including setting boundaries, celebrating small wins, and creating personalized routines. Emile also shares grounding practices like meditation and tapping to help high performers balance ambition with self-care and break free from self-sabotage. Episode Highlights: 00:00 - Episode trailer 02:47 - The Disconnect Between External Success and Internal Fulfillment 07:22 - The High Achiever Syndrome 11:07 - Celebrating the Simple Wins 14:56 - Understanding Where You're Creating From 17:30 - How Who You Believe You Are Shapes Your Actions 21:39 - Blind Spots of High Achievers 25:30 - Slowing Down to Speed Up 34:35 - Creating a Personalized Morning Routine 38:13 - Mastering Your Nighttime Routine 42:59 - Exploring Self-Sabotage Patterns in High Performers 46:05 - Why We Self-Sabotage 47:54 - The Emotional Connection to Money 52:35 - The Impact of Early Unacknowledged Dreams on Career Success About my guest: Emile Steenveld is a Human Behavior Expert and Transformative Guide, assisting countless individuals in their journey toward self-fulfillment and self-actualization. He leads his clients through deep internal shifts, addressing and mending long-held limiting beliefs and dismantling internal barriers that prevent them from realizing their full potential and success. His mission is to facilitate profound personal and professional transformation. He is dedicated to guiding individuals, particularly high achievers and entrepreneurs, on a journey of self-discovery, reconnection, and authentic expression. He recognizes the contemporary challenges faced by ambitious individuals who relentlessly pursue success, often at the expense of personal well-being and genuine relationships. His goal is to provide a comprehensive methodology that empowers them to overcome burnout, embrace self-acceptance, and excel both personally and professionally. Connect with Emile  Website: https://www.emilesteenveld.com/ Instagram: https://www.instagram.com/emilesteenveld/# YouTube: https://www.youtube.com/channel/UC0_RUx6ziLfhAw_6o1YVoXw Become a globally recognized Trainer and Speaker in just 90 days! https://www.speaktoleadblueprint.com/speaktoleadblueprint Want to manage your life the same way that helped me make $1 million by 28 and travel the world at the same time? Grab my FREE one-page Life By Design productivity planner below ⁠https://planner.morgantnelson.com/optinplanner Join the Dream Out Loud Facebook Community https://bit.ly/49QXClW Follow me on Instagram here https://www.Instagram.com/ Subscribe to my YouTube channel https://www.youtube.com/@morgantnelson

In this episode, we're diving into one of the most enduring cybersecurity challenges—weak passwords. We'll explore how poor password practices and identity management pitfalls leave organizations vulnerable to compromise. From understanding the most common mistakes to implementing effective defenses, we're breaking down what it takes to fortify your systems against attackers exploiting the weakest link. Sourceshttps://www.verizon.com/about/news/2023-data-breach-investigations-report?utm_source=chatgpt.comhttps://blog.1password.com/challenges-of-shadow-it/https://www.microsoft.com/en-us/security/security-insider/intelligence-reports/microsoft-digital-defense-report-2024?msockid=2e875ee0e1fe64d22f854aa6e0746523Blog: https://offsec.blog/Youtube: https://www.youtube.com/@cyberthreatpovTwitter: https://twitter.com/cyberthreatpovWork with Us: https://securit360.com

Use code ‘CALUMNOV10' to get 10 free meals from Hello Fresh: https://bit.ly/HelloFreshCJShow Sign up to Notion- https://www.notion.com/calumjohnson Join this channel to get access to perks: https://www.youtube.com/channel/UCuvjQYKukKjVyhSVxQibgOw/join Our Merch drops soon! Be 1 of 50 to get EXCLUSIVE ACCESS: https://bit.ly/StayDelusional2 Follow Us! Instagram: https://www.instagram.com/calumjohnson1?igsh=MTdzbHI1b3c1b2dmag== Twitter: https://x.com/calum_johnson9?s=21 Full episode with Ashley Fox https://youtu.be/yAPu61XYxKs Full episode with Tiffany Aliche https://youtu.be/4Hgy8WIJ7mg Full episode with Kayla Parrilla https://youtu.be/v4_FVm3bmyQ Full episode with Tricia Lee https://youtu.be/29Hv3GJAYUs 00:00 Intro 00:16 I was searching for approval! (Ashley Fox) 05:35 Shame will ruin your life (Tiffany Aliche) 15:12 Ignore the chaos and stay focused! (Kayla Parrilla) 19:05 I was dealing with RACISM but I succeeded anyway! (Tricia Lee) 24:23 I was being guided in this moment! (Ashley Fox) 33:09 The ONLY way to win (Tricia Lee) 36:32 How to ESCAPE negative emotions (Kayla Parrilla) 38:46 This is my darkest moment! (Tiffany Aliche) 43:39 This is what I'd tell my DAD! (Emotional!) About the episode In this episode, 4 successful women explain how they overcame challenging circumstances to build multi-million dollar businesses.

Join my upcoming Positive Intelligence© Program: https://www.vickibaird.com/positive-intelligence-purchase-vicki-baird I'm tackling the powerful duo of intuition and mindset—how combining them can bring you inner peace, success, and a little less head drama. And yes, I'm navigating the occasional tongue twister thanks to some dental adventures, but hey, challenges are just life's way of keeping it interesting, right? We'll discuss how understanding your intuitive strengths and building a supportive mindset can improve your business and life. I'll also introduce the Positive Intelligence© Program (a game-changer, seriously) and share practical tools, like PQ Reps, to help quiet your brain's noise and get back to focusing on what actually matters.  This episode is packed with tips for creating a calmer, more confident you while learning to embrace joy and success—whether you're running a business or just trying to run your day. Let's align that intuition with a mindset that works as hard as you do (but without all the stress). Give it a listen—you've got nothing to lose except maybe some mental clutter! ---

The Torah in Parashat Vayera tells the story of Hashem's destruction of the wicked city of Sedom and its neighboring towns. Before annihilating the region, Hashem informed Abraham Abinu of what He was planning to do. Abraham, the paragon of loving kindness and compassion, pleaded on Sedom's behalf, asking G-d to spare the city if He would find fifty righteous residents. G-d agreed, whereupon Abraham went further, pleading that the city be spared if there were even just forty-five worthy inhabitants. Hashem again consented, and Abraham then continued, begging for the city to be saved in the merit of even just thirty righteous people – and then twenty, and then ten. In the end, not even ten righteous people were found in Sedom, and so it and four other cities in the region were destroyed. The question arises as to why Hashem allowed Abraham to continue praying after presenting his initial request that the city should be spared in the merit of fifty righteous residents. Quite obviously, Hashem knew from the outset that there were not even ten people in Sedom worthy of being saved, not to mention twenty, thirty, forty, or forty-five. And yet, He allowed Abraham to continue pleading on the city's behalf, lowering the number all the way down to ten. Knowing that there were not enough righteous people in Sedom to spare the city, shouldn't Hashem have told Abraham to stop at the very beginning? Didn't He know that Abraham was wasting his time by continuing to pray for Sedom? The answer is that this question is predicated on a woefully mistaken assumption about the value and significance of prayer. It presumes that the value of prayer lies solely in its effectiveness in bringing the desired result. If the person will not attain that which he prays for, according to this logic, the prayer is a waste of time. But this is a grave mistake. When we pray for somebody else, we exercise our sensitivity muscle, so-to-speak. We become kinder, more compassionate, more caring, and more empathetic. By beseeching G-d on our fellow's behalf, we build our characters, as we develop within ourselves greater sensitivity for the needs of other people. Hashem therefore allowed Abraham to continue praying – not for Sedom's benefit, as the city was going to be destroyed anyway, but for Abraham's benefit, as the experience of praying made him even greater than he already was. So often we are called upon to pray for an ill patient, for those struggling to find a marriage partner or to have children, for the IDF soldiers, for the Israeli hostages, or for other beloved Jews in need. Painfully, our prayers do not always yield the results we wish for. We have all had the agonizing experience of praying regularly and passionately for an ill patient, and then finding out that the patient did not make it, Heaven forbid. Naturally, we feel very disappointed. And, some people become discouraged by these experiences, and begin questioning the value of their Tefilot. We must remember that prayer is always precious and significant, even if our request was not granted – not only because of how it builds our connection to Hashem, but also because how it builds our character, helping us develop into kinder and more compassionate people, who are sensitive to the needs and concern of others.

Ben and Rob unmask ‘Zodiac', David Fincher's tale of the hunt for the self-proclaimed “Zodiac Killer”, responsible for a spate of grisly murders across California from the late sixties through to the early seventies. Starring Jake Gyllenhaal, Mark Ruffalo and Robert Downey Jr, Fincher's ‘Zodiac' is a long, uncompromising film beloved by critics but widely ignored by audiences upon its release in 2007.But is this the most painfully personal movie in David Fincher's whole filmography? What drove Robert Downey Jr to compare working with Fincher to Soviet prison camps? Which scene in this darkest of movies is the one that most upset Ben? And has starting a Patreon butchered the usual 60 minute show available for free?CONSUUUME to find out all this and much MUCH more!BRAND NEW!!! We now have a Patreon with EXCLUSIVE content just for you starting at just ONE POUND a month - click the link below!Find us on your socials of choice or WATCH this episode at www.linktr.ee/everymovieeverpodcast

We hope you enjoy this podcast brought to you by Nick Conner and Danny Motta! We hope to cover all your favorite anime as well as any current noteworthy news! New episodes will be releasing each week, hope to see you at the next one! Danny Motta's Youtube Channel: https://www.youtube.com/@DannyMotta Nick aka Nchammer23's Youtube Channel: https://www.youtube.com/@NCHammer23 Otakus Anonymous Highlights Page: https://www.youtube.com/@otakusanonymoushighlights1698 Become a Patreon and get access to the Discord and livestreams! https://patreon.com/user?u=103227750 Buy OtakusAnonymous, NCHammer23, or Danny Motta merch: https://otakusanonymous.net/ Edited with love by: Stanktheanimedude 0:00 - Happy Halloween! 5:24 - We Can Do Better Than This. 17:37 - Scooby Doo Or Dictator? 38:03 - This Is A Compliment.. Right? 40:36 - Nick Loves Orb (Spoilers) 43:59 - Dandadan is FEET Focused 1:10:20 - Daima is Painfully Uncreative 1:19:48 - Blue Lock Got Better? 1:31:39 - This Week's Best Bois! 1:37:36 - The Boys Dox Each Other 1:43:37 - Brando Sando Talk 1:51:17 - This Week's Love Letters!

Spencer gives his immediate thoughts and opinions, from the field, Sunday after what could be looked at as a disaster game against the Bengals which included the season ending injury of Deshaun Watson. How bad was the injury? What's next for the Browns? How will the team look without him? Listen in NOW for the answers to all of these questions!

Text your thoughts directly to Rich. Starting out as a consulting business was a great way to learn what customers wanted, but caused problems as the other founders didn't want to give up the consulting work. About FulcrumFulcrum applies modern technology to manufacturing processes, helping makers focus on their craft and grow their businesses. The innovation of American manufacturers is critical to a better world, and Fulcrum aims to revitalize the industry with automation software.About AVL Growth PartnersAVL Growth Partners, founded in 2009, is the leading fractional Finance and Accounting firm supporting organizations in pivoting from growth to scale. AVL brings an experienced team of CFOs, Controllers, and Accountants to your organization, delivering transparent, strategic actions for short and long-term success. Transform your financial approach affordably with AVL, supporting companies coast to coast - get to know AVL Growth Partners at avlgrowth.com. (Sponsored)About SpringTime VenturesSpringTime Ventures seeds high-growth startups in healthcare, fintech & insurtech, and logistics & supply chain. We look for founders with domain expertise, forging a path with a truly transformative technology. We only invest in software-based businesses in the USA. We bring a people-focused approach, work quickly, and reach conviction independently. Our initial check size is $600k. You can learn more about us and our approach.    

Feeling trapped in a cycle of boredom? In this episode of Put Em On The Couch, Jason and Nelson invite you to Shock Your Boredom as they explore the rich history and surprising science behind this often-misunderstood emotion. From the Ancient Greeks' concept of Acedia to modern experiments that reveal our responses to boredom, discover why this feeling can be both challenging and transformative. Join us as we delve into shocking findings, including a study where participants opted for electric shocks over sitting in silence. With insights from comedian Louie CK and leading researchers like Dankert and Eastman, we'll uncover how boredom can be a catalyst for creativity and self-discovery. Tune in and learn how embracing boredom might just spark your next big idea! Don't forget to subscribe, rate, and review our podcast on your favorite platform. Share your thoughts on boredom and let us know how you cope with it on social media!

SHY GIRLS CAN BE CONFIDENT TOO! Confidence is not something you are born with, it's something that is practiced and learnt overtime through the power of self concept, positive affirmation, and detaching from the outside opinions of others. Would you believe me if I told you that before I learned to be the confident girl you see on your screen preaching self-worth and positive mindset, I used to be PAINFULLY shy. In today's episode, your host Brianna Gomez breaks down the best hot girl secrets to strength your self-concept in order to become confident, magnetic and release fear of judgement from others.Tune in to learn how to unlock your highest, hottest, & HAPPIEST self and truly GLOW UP from the inside and out.NEW COLORS OF MY CLOTHING COLLECTION JUST RELEASED!!!

In today's Daily Fix:Sony's Concord is live on Steam and PlayStation 5...and no one seems to care. On Steam, the game peaked with just 697 concurrent players during its launch weekend. By contrast, that's only 5% of what Suicide Squad: Kill the Justice League was able to pull durings that game's first weekend. In other news, Black Myth: Wukong sold an impressive 10 million copies in just three days, matching the pace of Nintendo's Pokémon Scarlet and Violet (which is technically two different games). It's also the most-played single-player game of all time on Steam, overtaking Cyberpunk 2077, and is just behind PUBG as most-played game across all genres in terms of concurrent players. And finally, Nintendo has announced a pair of showcases for tomorrow morning, starting at 7am Pacific Time. And, no, there won't be a Switch 2 there, and also we don't know when Nintendo will announce one.

In episode 579, Mike and James invite Nicholas Dugré back to the podcast to talk about the evidence around the benefits and harms of a new class of medications for the acute treatment of episodic migraines. Ubrogepant is an oral calcitonin gene-related peptide antagonist. In this case, amazing as it may sound, there are triple […]

Okay whats one thing that you wish someone would have told you before we got married..

Joe Heschmeyer picks apart a popular 101 video on Martin Luther’s revolt, pointing out many painfully common factual inaccuracies. Transcription: Welcome back to Shameless Popery. I’m Joe Heschmeyer. So we’re talking about the reformation, particularly the beginning of the Reformation with Martin Luther. I think a lot of people feel like they have a pretty good grip on the basic story, and the basic story tends to go something like this. On October the 31st of 1517, a Catholic monk and priest named Martin Luther nailed 95 theses to the door of the church in Wittenberg, German…

My how THE TURN tables! Chad Zumock has seemingly worn out his welcome for the third time as a guest on the MLC podcast with Kevin Brennan. Kevin unloads on Chad in a tirade as nonsensical as it was unexpected, referencing everything from crashing his car to wishing Chrissy Mayr's baby death. When Kevin is done with you, he's done. Pat Dixon gets caught in the crossfire. Michael Ray Bower aka Donkeylips is depressed about something that may or may not be happening to him. It's hard to tell when paranoia seems to run his life. Heather Gillespie has had her baby taken away from her, and she has a lot of advice and information for everyone else on how to live while she waits to earn that baby back! ...

**Use our code "VAULT10" for 10% off your next purchase at Manta Sleep: http://tinyurl.com/bdetkeuk Miami Dolphins wide receiver Tyreek Hill recently appeared on The Pivot Podcast and shared something about the Ravens that was painfully true to many in Baltimore. Also, we'll cross compare Daniel Jeremiah's “Blue chip” players he has listed for both Baltimore and the Chiefs. On this edition of the Vault, Sarah & Bobby dive into that along with the following: Combing through free agency rumors: Patrick Queen, Joe Flacco, etc. Reflecting on a wild Lamar Jackson realization from a year ago And more... Interested in supporting the show? Check out what we're now offering on Patreon: https://bit.ly/3RorjU2 Hit us up: BaltimoreRavensVault@gmail.com Interested in advertising on this podcast? Email sales@bluewirepods.com Learn more about your ad choices. Visit podcastchoices.com/adchoices