Podcasts about painfully

Allen covers the debate over Chinese wind turbines in Europe, from data security concerns and unfair subsidies to the risk of trading one energy dependency for another. Sign up now for Uptime Tech News, our weekly email update on all things wind technology. This episode is sponsored by Weather Guard Lightning Tech. Learn more about Weather Guard’s StrikeTape Wind Turbine LPS retrofit. Follow the show on Facebook, YouTube, Twitter, Linkedin and visit Weather Guard on the web. And subscribe to Rosemary Barnes’ YouTube channel here. Have a question we can answer on the show? Email us! Wind energy is one of Europe’s great strengths. Providing twenty percent of European electricity today. Over half by 2050. That’s the plan. Competitive. Homegrown. Quick to build. Almost every wind turbine spinning in Europe today was made in Europe. By European companies. Assembled in European factories. Hundreds of factories across the continent make components for wind turbines. Over Four hundred thousand Europeans punch the clock in wind energy. Every new turbine generates sixteen million euros of economic activity. And this week, proof of that investment. In Germany, the He Dreiht offshore wind farm just sent its first power into the grid. Nine hundred sixty megawatts. Germany’s largest offshore wind farm. VESTAS turbines standing one hundred forty-two meters tall. Sixty-four turbines total. All commissioned by summer 2026. NILS DE BAAR of VESTAS said the fifteen megawatt turbine sets new standards in offshore wind power. European technology. European manufacturing. European energy. In Ireland, more European investment. SSE and FUTURENERGY IRELAND tapped NORDEX to build the Wind Farm in County Donegal. Twelve turbines. Sixty megawatts. One hundred thirty-eight million dollars. Forty thousand Irish homes powered when those blades turn in 2027. And in Scotland and Italy, floating wind is consolidating. NADARA is acquiring BLUEFLOAT ENERGY’s stake in ten floating offshore projects. BROADSHORE. BELLROCK. SINCLAIR. SCARABEN. Nearly three gigawatts of floating wind now under single European ownership. Today’s wind farms save Europe one hundred billion cubic meters of gas imports every year. In Britain alone, consumers saved one hundred four billion pounds between 2010 and 2023. That’s after factoring in the cost of building the wind farms. Wind means lower energy bills. Wind means independence. But here comes the temptation. Chinese turbines are cheaper. Much cheaper. And in times of strained budgets and rising costs… That’s hard to ignore. GILES DICKSON is the CEO of WINDEUROPE. He says… Think about what you’re buying. The European Commission launched an inquiry last year. They suspect Chinese manufacturers offer prices and payment terms backed by unfair government subsidies. European manufacturers can’t legally offer the same deferred payment deals. OECD rules won’t allow it. Then there’s energy security. Europe just weaned itself off Russian gas. Painfully. Expensively. Three years later, high energy prices still drag on the economy. Does Europe want another dangerous dependency? This time on imported equipment instead of imported fuel? And as Giles points out – a modern wind turbine has hundreds of sensors. Hundreds. Gathering performance data. Monitoring operations. European law prohibits exporting that data to China. But Chinese law allows Beijing to require Chinese companies to send data home from overseas operations. There’s a contradiction. Someone’s going to break the law. And those sensors? They don’t just collect data. They can control equipment. The European Union and NATO are voicing concerns. The wind industry has invested over fourteen billion euros in new and expanded European factories in just the last two years. That’s commitment. That’s confidence. And the rest of the world is taking notice. In Japan, FAIRWIND just signed a strategic partnership with WIND ENERGY PARTNERS in YOKOHAMA. MATT CROSSAN, FAIRWIND’s Asia Pacific Director, said Japan’s wind sector is still young compared to Europe. But government support and investment are driving expansion. They want European expertise. European experience. European standards. Wind energy is the last strategic clean tech sector with a truly European footprint. The last one. Solar panels. Batteries. Electric vehicles. Those have already migrated elsewhere. But Wind remains. For now. Four hundred forty thousand workers. Two hundred fifty factories. Fourteen billion euros in new investment. One hundred billion cubic meters of gas imports avoided every year. Germany’s largest offshore wind farm now feeding the grid. Ireland building new capacity. Scotland consolidating floating wind. Japan seeking European partners. Europe can buy cheaper today. Or build stronger tomorrow. GILES DICKSON is sounding the alarm. But, will Europe listen? That's the wind industry news on the 1st of December 2025.

Jase gets lovingly roasted by a self-proclaimed “dork” who calls him out for talking too much and he has to admit, it's true. The listener's tongue-in-cheek “Unashamed Drinking Game” exposes all the guys' funniest and worst habits, leading Zach to suspect it might be a Robertson family member secretly trolling them. The guys pivot to a lively discussion about why unity in Christ matters more than denominational lines and how Jesus' resurrected body defies description yet fills us with hope for our own resurrection. In this episode: John 19; 1 Corinthians 15; 1 John 3; Romans 4; Romans 8; Hebrews 11; Genesis 22. “Unashamed” Episode 1208 is sponsored by: Stand firm for values that matter. Join the fight & give today at https://www.frc.org/unashamed https://tomorrowclubs.org/unashamed — For a limited time, all donations to this “Binga Blitz” will be matched! Join us now and double your impact! https://chministries.org/unashamed — See why Christians are ditching health insurance for good. Get a simpler alternative at half the cost! https://myphdweightloss.com — Find out how Al is finally losing weight! Schedule your one-on-one consultation today by visiting the website or calling 864-644-1900. https://andrewandtodd.com or call 888-888-1172 — These guys are the real deal. Get trusted mortgage guidance and expertise from someone who shares your values! http://unashamedforhillsdale.com/ — Sign up now for free, and join the Unashamed hosts every Friday for Unashamed Academy Powered by Hillsdale College Listen to Not Yet Now with Zach Dasher on Apple, Spotify, iHeart, or anywhere you get podcasts. Check out At Home with Phil Robertson, nearly 800 episodes of Phil's unfiltered wisdom, humor, and biblical truth, available for free for the first time! Get it on Apple, Spotify, Amazon, and anywhere you listen to podcasts! https://open.spotify.com/show/3LY8eJ4ZBZHmsImGoDNK2l Chapters: 00:00-04:33 Jase isn't a great listener 04:34-18:46 Churches need to unify, not divide 18:47-26:42 The Unashamed “drinking game” 26:43-31:35 Jase's chickens come home to roost 31:36-40:26 No words could describe Jesus' resurrected body 40:27-50:10 Abraham got it wrong & still got it right 50:11-57:06 How to grow your faith step-by-step — Learn more about your ad choices. Visit megaphone.fm/adchoices

In a floating city where the rich discard their waste—and people—Rudo is framed for murder and thrown into the Pit, where a hellscape of mutated trash beasts dwell. To survive, he must wield a new power and join the Cleaners. Rudo doesn't just aim to battle monsters, but the corrupt who cast him into Hell. We're going to take a brief hiatus to let the next season catch up to us, then we will go back to Gachiakuta. Our next anime is Secrets of the Silent Witch. Humans couldn't handle magic without chanting until Monica Everett, the Silent Witch and one of the Seven Sages, made unspoken magecraft possible. Painfully shy, she enjoys seclusion. One day, Louis Miller, the Barrier Mage, delivers the king's order: Go undercover at a prestigious school for nobles to guard the second prince. Get ready for her silent mission to begin!

This week, Josh and Bob tackle the hidden beliefs that hold leaders back — inspired by a recent Harvard Business Review article. From perfectionism and ego to the inability to say “no,” they unpack the mental traps that quietly sabotage leadership growth.Through stories, humor, and brutal honesty, they dig into what it really means to lead without making it about you. Whether it's learning that not everyone's wired like you, realizing you're the ceiling for your team, or finally getting comfortable saying “no,” this episode is a mirror for every leader who's brave enough to look.It's part confession, part coaching session — and all real talk about what it takes to lead in today's world.Bob's Book Recommendation: Death March: The Complete Software Developer's Guide to Surviving "Mission Impossible" ProjectsJosh's Book Recommendation - The Mythical Man-Month: Essays on Software Engineering, Anniversary EditionThe Hidden Beliefs That Hold Leaders Back - The HBR article that inspired this episodeStay Connected and Informed with Our NewslettersJosh Anderson's "Leadership Lighthouse"Dive deeper into the world of Agile leadership and management with Josh Anderson's "Leadership Lighthouse." This bi-weekly newsletter offers insights, tips, and personal stories to help you navigate the complexities of leadership in today's fast-paced tech environment. Whether you're a new manager or a seasoned leader, you'll find valuable guidance and practical advice to enhance your leadership skills. Subscribe to "Leadership Lighthouse" for the latest articles and exclusive content right to your inbox.Subscribe hereBob Galen's "Agile Moose"Bob Galen's "Agile Moose" is a must-read for anyone interested in Agile practices, team dynamics, and personal growth within the tech industry. The newsletter features in-depth analysis, case studies, and actionable tips to help you excel in your Agile journey. Bob brings his extensive experience and thoughtful perspectives directly to you, covering everything from foundational Agile concepts to advanced techniques. Join a community of Agile enthusiasts and practitioners by subscribing to "Agile Moose."Subscribe hereDo More Than Listen:We publish video versions of every episode and post them on our YouTube page.Help Us Spread The Word: Love our content? Help us out by sharing on social media, rating our podcast/episodes on iTunes, or by giving to our Patreon campaign. Every time you give, in any way, you empower our mission of helping as many agilists as possible. Thanks for sharing!

Start building your first AI Agent with Lindy (no code!): https://go.lindy.ai/calum-johnsonGet our free AI Agents 2025 Playbook: https://clickhubspot.com/c73275Follow Us!https://www.instagram.com/calumjohnson1/https://x.com/calum_johnson9Guest: @neilpatel Timestamps00:00 Intro04:10 Why this is the new AI Gold Rush (and how to seize it)09:37 Humans + AI: The unique approach to win in the AI era14:22 How Neil spotted his $100M idea before anyone else19:01 Step 1: Find simple, realistic, high-demand ideas (Works every time)24:35 The first 7-figure AI business idea: Build AI Agents for companies30:55 How to pitch and sell your first AI client34:58 The second AI business idea: Launch an AI Content Agency39:20 Why 100% AI content fails (and the human fix)44:13 The third AI business idea: Generative Engine Optimization (GEO)54:01 The future belongs to these type of people....About the EpisodeNeil Patel is one of the world's top marketers and founder of NP Digital, a $100M marketing agency serving global brands. In this episode, we break down three proven AI business ideas you can start right now (these are 7-8 figure ideas).

So easy to fix, but they are morons, it's Dynamite. Oh do we get a joe explanation? Well Hangman will run in right? Stat trys to have a personality Why is Mox and Darby there? Women tag tournament? Tony stares at the camera Sasha yells at a puppet 90210 Briscoe and Cox again???? He is the world champion. Pin. Clean Subscribe on patreon.com/LingusMafia for ad-free and video versions of the show, exclusive PPV/PLE reviews and bonus shows including every Wrestlemania, SummerSlam, Royal Rumble, Survivor Series, and Saturday Night's Main Event ever. Get access to over 10 years of podcasts! Stay connected: All our social media (@LingusMafia) links can be found here: https://linktr.ee/lingusmafia We have merch! Shirts, hoodies, stickers and more: lingusmafiashop.printify.me/ Drop us an email with comments or questions: lingusmafia@gmail.com Check our YouTube out at Wrestle Lingus Show! Remember to leave a comment and rate the show wherever you get your podcast from, we gotta get the word out there, we aren't too proud to beg, please? Hosted by Simplecast, an AdsWizz company. See pcm.adswizz.com for information about our collection and use of personal data for advertising.

Dublin has recently spent €600 million on its bus service and is planning on spending €3 billion more. Buses and the service they provide have improved greatly and money has certainly been spent. Even so, the bus service is inadequate and journey times have not improved. All to discuss with Conor Faughnan, Transport Commentator.

This week on Thoughts Off The Stem, we're lighting up the truth about THC potency fraud in the legal cannabis market. Some companies are intentionally mislabelling THC levels, and Health Canada's response? Painfully slow.We'll dig into why regulators ignored industry insiders' warnings, how their “public health first” stance doesn't match their actions, and why the 10mg edible cap might actually be part of the problem.Could more realistic potency ranges stop shady labeling and give consumers what they really paid for? Let's talk corporate greed, government red tape, and the real impact on cannabis culture.Like, Share, Subscribe, leave a Comment and visit Tots420.comYou can read the article referenced on the show here:https://stratcann.com/news/two-potluck-chillows-products-recalled-in-ontario-due-to-excessive-variability-and-exceeding-10-mg-thc-limit/THC label lies,Health Canada controversy,cannabis industry Canada,cannabis mislabelling,THC potency,cannabis comedy podcast,Thoughts Off The Stem,Justin Barone,weed podcast,cannabis culture,cannabis podcast Canada,cannabis regulation,edible THC limit,legal weed Canada,cannabis news,weed humor,cannabis industry expose,THC testing,cannabis consumers,Health Canada,funny cannabis podcast,insight,unfiltered,real talk,comedy podcast,cannabis education

A raw, and painfully honest conversation about mental illness, depression, and suicide. Told through my real experience. Crystal dives deep into the messy truth of what it's like to live inside your own head when it turns against you. Equal parts educational, emotional, and uncomfortably relatable, this episode sheds light on the hidden battles people fight every day… reminding you that even in the darkest moments, you're not alone. Text HOME to 741-741 to speak to a trained counselor 24/7 for FREE!

Rayan, Wilf, and Ivan return to review Channel 4's brand new reality competition, by the makers of The Traitors - The Inheritance. From lacking strategies to main character syndrome, and from Queen Elizabeth to less endearing cast members, they consider what makes this a painfully quintessentially British show. Follow us on social media for more exclusive content! Hosted on Acast. See acast.com/privacy for more information.

Today we run down another solid session for the US market, with enthusiasm in abundance on the Nvidia investment in Intel, a development we discuss with Saxo Equity Strategist Ruben Dalfovo, together with the surge in ASML and Crowdstrike yesterday. Also, a preview of some names reporting next week, including recent darling Micron, key developments in FX as the US dollar is on the comeback path and the JPY churns post-BoJ. Today's pod hosted by Saxo Global Head of Macro Strategy John J. Hardy. Links discussed on the podcast and our Chart of the Day can be found on the John J. Hardy substack (with a one- to two-hour delay from the time of the podcast release). Read daily in-depth market updates from the Saxo Market Call and the Saxo Strategy Team here. Please reach out to us at marketcall@saxobank.com for feedback and questions. Click here to open an account with Saxo. Intro and outro music by AShamaluevMusic

I'm sad Listeners. Today, Charlie Kirk was shot and killed. I recorded the majority of this podcast 2 days ago. I think it has merit but I'm not gonna shout this one too loud beyond how I feel for Charlie and his poor family. Of course, I'm grateful for all your listening time and honored.  What I want you all to know is that I'm going to keep speaking. Our Lord tells us to "fear not," and I will not be afraid. God bless you all.   

Get $350 off the EightSleep Pod 5 Ultra— https://www.eightsleep.com/calumjohnsonFollow Us!https://www.instagram.com/calumjohnson1https://x.com/calum_johnson9Guest: https://www.linkedin.com/in/drbenjaminhardyTimestamps00:00 Intro03:15 Past vs future as tools (end stagnation fast)10:11 Your future self is real (the Harvard insight)12:44 10x your income without working harder!16:04 Set an “impossible” goal — then shorten the timeline18:44 Sponsor: The Eight Sleep upgrade that fueled output20:39 What a true stretch goal looks like (you don't know how)23:26 18 months to a $220K book deal (Ben's path)25:35 Selective attention vs inattentional blindness (find the path)33:56 Your goals are ruining your dreams!36:50 Identity is the reason you are broke!42:14 Survival mode? Journal a new future, then speak it51:33 The "subtract-then-scale" playbook59:49 Are you a Buyer or Seller: Know when to walk away!1:11:28 You must think bigger (The Billion Podcast)About the Guest / About the EpisodeDr. Benjamin Hardy is a bestselling author whose books have sold millions of copies, he has coached multiple 8 figure entrepreneurs on how to scale their companies. In this episode, we build a practical roadmap for stuck founders: set an “impossible” goal, shrink the timeline, raise your floor, operate from identity, and become the buyer (not the seller) so you can 10–100x your business and your life

Want more MTM Vegas? Check out our Patreon for access to our exclusive weekly aftershow! patreon.com/mtmvegas Want to work with us? Reach out! inquiries at mtmvegas dot com Episode Description This week Strat's owners released their earnings including occupancy rates for June and it isn't looking good. What is going well at the property and why is it struggling to attract visitors. Are young people today overlooking the Strip resort for something nicer? What bright spots are there for the Strip icon? In other news the A's stadium has reached another milestone. Concrete! We also discuss: Bottled Blonde vs. Ole Red views, Stage Door/Battista's delays, Suns Out leaving Resorts World, the Labubu rave, Adventuredome's current state, ATMs at table games, how slots actually work and why Downtown's most classic casino is losing all of its table games. Episode Guide 0:00 Labubu rave hits Vegas 1:00 Primm unveils brand new marquee, new hope? 3:26 New restaurants and more - How Primm is trying to rebrand 4:26 Suns Out Buns Out is gone at Resorts World 5:51 Resorts World's new Smashburger concept 7:05 Golden Gate announces drastic casino changes 9:52 Adventuredome is looking very nice & other takeaways 12:40 Bottled Blonde vs. Ole Red fountain views 14:13 Delays affecting Battista & Stage Door's renovations 16:20 ATMs at table games? 18:19 How slot machines actually work 19:33 Strat is having a horrid summer 21:30 Top of the World restaurant / rotating restaurants 22:52 The A's pour concrete! Each week tens of thousands of people tune into our MtM Vegas news shows at http://www.YouTube.com/milestomemories. We do two news shows weekly on YouTube with this being the audio version. Never miss out on the latest happenings in and around Las Vegas! Enjoying the podcast? Please consider leaving us a positive review on your favorite podcast platform! You can also connect with us anytime at podcast@milestomemories.com. You can subscribe on Apple Podcasts, Google Podcasts, Spotify or by searching "MtM Vegas" or "Miles to Memories" in your favorite podcast app. Don't forget to check out our travel/miles/points podcast as well!

Want more MTM Vegas? Check out our Patreon for access to our exclusive weekly aftershow! patreon.com/mtmvegas Want to work with us? Reach out! inquiries at mtmvegas dot com Episode Description This week Strat's owners released their earnings including occupancy rates for June and it isn't looking good. What is going well at the property and why is it struggling to attract visitors. Are young people today overlooking the Strip resort for something nicer? What bright spots are there for the Strip icon? In other news the A's stadium has reached another milestone. Concrete! We also discuss: Bottled Blonde vs. Ole Red views, Stage Door/Battista's delays, Suns Out leaving Resorts World, the Labubu rave, Adventuredome's current state, ATMs at table games, how slots actually work and why Downtown's most classic casino is losing all of its table games. Episode Guide 0:00 Labubu rave hits Vegas 1:00 Primm unveils brand new marquee, new hope? 3:26 New restaurants and more - How Primm is trying to rebrand 4:26 Suns Out Buns Out is gone at Resorts World 5:51 Resorts World's new Smashburger concept 7:05 Golden Gate announces drastic casino changes 9:52 Adventuredome is looking very nice & other takeaways 12:40 Bottled Blonde vs. Ole Red fountain views 14:13 Delays affecting Battista & Stage Door's renovations 16:20 ATMs at table games? 18:19 How slot machines actually work 19:33 Strat is having a horrid summer 21:30 Top of the World restaurant / rotating restaurants 22:52 The A's pour concrete! Each week tens of thousands of people tune into our MtM Vegas news shows at http://www.YouTube.com/milestomemories. We do two news shows weekly on YouTube with this being the audio version. Never miss out on the latest happenings in and around Las Vegas! Enjoying the podcast? Please consider leaving us a positive review on your favorite podcast platform! You can also connect with us anytime at podcast@milestomemories.com.  You can subscribe on Apple Podcasts, Google Podcasts, Spotify or by searching "MtM Vegas" or "Miles to Memories" in your favorite podcast app. Don't forget to check out our travel/miles/points podcast as well!

Why do so many modern songs feel ear-piercing lately? In this episode, we dive deep into reasons why today's mixes are getting brighter, louder, and in some cases Painfully harsh. Were breaking down the evolution of mixing trends, the impact of digital gear vs analog.If you're a producer and want to get on Colossal, and sell more beats, register now at colossal.fm/producers - and it's free, no subscription costs!➡️https://colossal.fmhttps://www.instagram.com/colossal_apphttps://www.tiktok.com/@colossal.apphttps://www.youtube.com/@colossal-app➡️ Get Our Rosetta Plugins: https://cutt.ly/RwAEmuRF➡️ Our Site: https://www.helpmedevvon.com Please subscribe to our YouTube and rate our podcast it helps us a lot!➡️ https://linktr.ee/mystudionerdsFollow Ushttps://www.instagram.com/helpmedevvon➡️Join this channel to get access to perks:https://www.youtube.com/channel/UCGyDjbX9F9ARt_8sgv5kGDg/joinFollow The CastDevvon Terrellhttps://instagram.com/helpmedevvonLJhttps://instagram.com/prodbyljeanHere is L.Jean channel! https://youtube.com/@SweataWeathaCourtney Taylorhttps://instagram.com/officialcourtneytaylor#podcast #mixing #mastering

News with Sean 7-17-2025 ...Be Prepared it is going to Painfully Hot Again

Do you find stripping and nudity empowering or degrading? Burlesque performer Dita Von Teese says feminism is about respecting each other's choices, even if they wouldn't be your own.In this chat with Fearne, Dita charts her life from working in strip clubs in the 90s, to performing in sold out burlesque shows now. Painfully shy as a kid, Dita explains how turning herself into a femme fatale helped her feel confident and powerful in her own skin.Fearne and Dita talk about the joy of getting older and understanding more about how to handle your sexuality, and Dita reveals how she makes herself feel more sensual if she's not particularly in the mood...There's also advice about how to own your unique look and characteristics, and be more assertive in using your voice.Dita's new show, Diamonds and Dust, is on at London's Emerald Theatre now! Dita is also returning to the UK's grand stages in early 2026 with her most enchanting show to date: Nocturnelle. If you liked this episode of Happy Place, you might also like: Munroe Bergdorf Florence Given Zandra Rhodes Hosted on Acast. See acast.com/privacy for more information.

Michael Kramer from Mott Capital Management and Reading The Markets on the first half of the year - market recovery has mostly been surprising (0:35). Magnificent 7 now The Troubling 3 (2:05). Regrets from owning Amazon over Nvidia? (9:05). AI has more promise in healthcare (13:25). Painfully long Tesla (16:15). Poor liquidity in marketplace (18:50). US economy health most important, especially employment (24:00).Show Notes:Wall Street ends at a record high after U.S-Vietnam trade dealFlirting With StagflationThe Bond Market Just Issued A Warning On UnemploymentThe Stock Market Looks Great, And That's The TrapThe Dollar Is At A Critical Juncture As Major Currencies Eye BreakoutsEpisode transcriptsFor 20% off access to all investing groups, stock scores and dividend grades, subscribe now: seekingalpha.com/subscriptions

Green Noise Records is proud to present Prime Primitive: 1976–1977, a vital archival release from legendary New York City punk band Testors, available May 30, 2025. Capturing the essence of the band's earliest studio and live recordings, this LP offers a long-overdue look at one of the rawest and most uncompromising acts to emerge from the original NYC punk underground. Founded by Sonny Vincent in 1975, Testors took shape in the grimy backrooms and sweat-soaked stages of Max's Kansas City and CBGB—two epicenters of the East Village scene that gave rise to the Ramones, Richard Hell, the Heartbreakers, and Television. Testors stayed firmly committed to the fire and fury of pure rock'n'roll, leaving behind a trail of scorched earth and unforgettable shows—including a tour with the Dead Boys that spread their chaotic energy nationwide. Far from lacking recorded material, Testors had a wealth of songs and studio sessions, but due to their fiery temperament and their desire to burn every bridge in sight they simply rejected the compromises demanded by record companies. Their often violent, stripped-down sound helped shape the American punk blueprint that thousands of bands would follow. Testors erupted on the New York scene at the epicenter of the punk rock explosion. Absolutely one of the best raw, catastrophic, and shocking bands to ever grace the stages of Max's Kansas City and CBGB's during the mid-70's punk heyday, Their incendiary sound consisted of dueling guitar noise and Sonny Vincent's street poetry. Testors created music that stands the test of time. Painfully delicate, unabashed, unrepentant, explosive, and savagely persuasive. Their earliest recordings are considerably tougher, louder and more desperate than any other music made at that time (or since) and after you let it latch onto your soul, you're forever indebted to New York's toughest, rawest, REALest punk band for the rest of your fucking life. It's a crime against history that the Testors never got their due: a band like this could and should have changed everything. Prime Primitive captures Testors the way they were meant to be heard—on vinyl, no CDs, no digital downloads, no AI. Just the real-deal slab,  in an exclusive silver foil jacket for true believers to have and to hold. Adding to the celebration, a newly unearthed video titled Testors – 1979—featuring rare 16mm footage of the band and candid backstage moments with Testors, the Dead Boys, and Johnny Thunders at Irving Plaza—will be available via the Green Noise Records YouTube channel. Directed and edited by Robert Luttrell, with words and music by Sonny Vincent, the video offers a raw visual snapshot of an era that refuses to fade. Prime Primitive is a sonic time capsule of Testors at their most volatile and vital. Side A compiles nine of their early studio recordings from 1976–77—taut, feral tracks that hiss with desperation and attitude—while Side B delivers five ferocious live cuts that find the band tearing through their set with unhinged intensity. All tracks were newly remastered for this release by Timothy Stollenwerk (Stereophonic Mastering) in close collaboration with Sonny Vincent, ensuring maximum punch, grit, and clarity. Every song was written, arranged, and produced by Vincent, whose compositions and streetwise lyrics remain as bracing now as they were nearly fifty years ago. These aren't just recordings—they're artifacts of a cultural explosion, finally given the fidelity and sequencing they always deserved. D I S C O V E R Bandcamp:https://sonnyvincenttestors.bandcamp.com/Instagram:https://www.instagram.com/sonnyvincent77/Label:https://store.greennoiserecords.com/products/testors-prime-primitive-1976-1977-new-lpFacebook 1:https://www.facebook.com/sonnyvincentprivatepage.1?mibextid=ZbWKwLFacebook 2:https://www.facebook.com/p/Sonny-Vincent-100057660871484 Checkout my YouTube Channel with long form interviews from the Subversives | the History of Lowest of the Low.

痛并快乐着/ 28th May maayot Featured Story安娜：这周末一起去吃麻辣火锅吗？张丽：你不是不能吃辣吗？安娜：我的胃受不了很辣。但是嘴巴还是想吃。张丽：对啊，上次看到你边吃火锅边哭。安娜：我是“痛并快乐着”！张丽：哈哈，对我来说，我是“无辣不欢”。Do you know ? You can slow down or speed up the audio to match your listening level at maayot. You can get personal feedback from real teachers—just click the

Send us a textWhy is it that the tiniest injuries hurt the MOST? In this episode, we break down the dumbest, most annoying, and surprisingly painful injuries that life throws at you—stubbed toes, paper cuts, mystery bruises, and more. These aren't heroic battle scars... they're “I bumped into the coffee table again” injuries. Relatable? Painfully.Grab a drink, cringe with us, and share your own ridiculous injury stories in the comments. Let's bond over the pain that shouldn't have hurt this bad.One Drink PodcastOne topic for One Drink. Topics are different and fun unlike the main media.Gods N GladiatorsCustom Clothing / Wall ArtDisclaimer: This post contains affiliate links. If you make a purchase, I may receive a commission at no extra cost to you.https://www.youtube.com/channel/UC-ytHeiGG6VND5GUmoWij-A

You're not going to like this—but I've come to believe it's true:We're not teaching people empathy.Not in business school.Not in PhD programs.Not in most classrooms.We're teaching performance.Frameworks.Strategy.Execution.But not how to read a room.Not how to ask:“How is this person actually feeling right now?”Or: “How do I show up in a way that helps, not harms?”I used to think empathy was soft.I now believe it's the core skill that makes or breaks your success—in research, in business, and in life.The thing is… I'm naturally empathetic.And for a long time, I assumed everyone else was too.That people were good.That they meant well.That if I showed up with kindness, it would be returned.That was wrong. Painfully wrong.Empathy doesn't work on everyone.Some people take advantage of it.Some people weaponize your kindness.And the more you give, the more they take.But that doesn't mean you give up on empathy.It means you learn to use it wisely.Empathy is not weakness.It's not being a doormat.It's not endlessly giving.It's knowing when to give.And when to walk away.In my house, we talk about feelings.We ask, “How do you think they felt?”And I've seen the difference that makes with my kids.A little empathy changes everything:How you teach.How you parent.How you lead.How you breathe in a room that doesn't fully accept you.But here's the catch:Empathy only works if you also protect yourself.So if you're the kind of person who gives…Who senses everything…Who gets crushed when you're ignored or dismissed—This is your permission:You are not wrong. You are not soft. You are not naive.You're reading the world at a deeper level.Just make sure you don't burn out trying to fix it.Empathy is your superpower.But like all power, it needs boundaries.

If you're waiting for a breakthrough moment, a sign from the universe, or the “perfect” conditions to create change… this episode is your wake-up call.Today I share with you the actual framework I use (and teach) to create meaningful, long-lasting change. No fluff. No hype. Just reality, honesty, and the painfully simple steps we often avoid because they seem too obvious or not enough.Here's what we cover:Why change starts with meeting life exactly as it is.The power of looking back—not to dwell, but to understand how you actually got here.Looking forward—not with fantasy, but clarity on what you'd love life to feel and look like.The unsexy magic of taking the next simple step. Then the next. Then the next.Why playing the long game matters way more than chasing quick wins.If you've been feeling stuck, overwhelmed, or like change is too hard—get this episode in your earballs. Because the truth is, it's not that complicated. It's just uncomfortable. And most of us would rather stay in a known discomfort than face the unknown of change.But if you're willing to show up, get honest, and take those painfully simple steps, everything shifts.Buy my book, Authentic - coming home to your true self - AUS, EUR, USACoach with me 1:1 - Book a 20-minute call nowThe Good Life - sign up nowFree Wheel of Life Assessment - take the test nowSunday Meds - live event by the beach, June 22ndWrite into us - let us know what you want to hearwww.katjohn.com.auSupport the show

Here is a narration of my latest ‘a calm presence', inspired by this quote from Indy Johar's May 12th, 2025 Substack posting, The Stickiness of Want  - And the Systemic Amnesia Behind It :We—you, me, everyone in this room—are the last generation with viable agency before degenerative volatility locks us into conflict and collapse. The window is painfully small but gloriously open.'This posting was written while traveling in India and Japan in April and May of 2025.The narrated version was recorded in one take on May 21, 2025 on the streets of Hakone-Yumoto, Japan with the Haya River and lively birdsong in the background.See the Transcript of this episode for the complete posting.  *END NOTES FOR ALL EPISODESHey conscient listeners, I've been producing the conscient podcast as a learning and unlearning journey since May 2020 on un-ceded Anishinaabe Algonquin territory (Ottawa). It's my way to give back.In parallel with the production of the conscient podcast and its francophone counterpart, balado conscient, I publish a Substack newsletter called ‘a calm presence' see https://acalmpresence.substack.com. Your feedback is always welcome at claude@conscient.ca and/or on social media: Facebook, Instagram, Linkedin, Threads or BlueSky.I am grateful and accountable to the earth and the human labour that provided me with the privilege of producing this podcast, including the toxic materials and extractive processes behind the computers, recorders, transportation systems and infrastructure that made this production possible. Claude SchryerLatest update on March 26, 2025

Join this channel to get access to perks:https://www.youtube.com/channel/UCuvjQYKukKjVyhSVxQibgOw/joinFollow Us!https://www.instagram.com/calumjohnson1/https://x.com/calum_johnson9https://www.youtube.com/@UCf8gbZ4ZyNcc9WIOOEEfpSQ Timestamps00:00 Intro04:48 What makes Malique's message resonate so deeply07:16 “She went without so we could have” (A Mother's Sacrifice!!)16:24 The 300 job rejections — and the moment that changed everything18:25 “No one hired me, so I hired myself”22:40 The first viral video: 100K views in 24 Hours!24:47 Step 1: Wake up — God wants you see this!31:42 How to know you're asleep at the wheel36:26 Signs you're living someone else's dream38:53 Your mom will need you one day — and you must be ready44:09 The real reason your life isn't changing47:31 Step 2: Self-audit and get still50:59 You can't hear God if you're never quiet53:33 How journaling & prayer unlock your purpose58:49 Step 3:"Go Ghost: Upgrade Your Life In Silence" (this will change everything!)1:01:15 Why you must protect your dreams in the early stages1:13:34 Step 4: Build your discipline code (habits, systems, momentum)1:22:45 The 10% rule: how to win on your worst days1:28:08 Why the gym is the most important discipline1:34:19 Identity shift: "I am purpose now"1:38:23 “Life is not about what we achieve. It's about what we overcome.”About The GuestMalique David Lewis is a speaker, creator, and founder of 'Purpose Driven'—a movement empowering men to live with intention, discipline, and faith. From poverty in Miami to building a platform with millions of views, his story is one of spiritual clarity, community leadership, and relentless self-belief.

Post-Dispatch sportswriter Tom Timmermann joined columnist Jeff Gordon to discuss the agonizing end to the Blues-Jets series, but also the better times to come. Find more podcasts from the Post-Dispatch. Subscribe to the Post-Dispatch.

(00:00) The crew recaps the Celtics' Game 4 win over the Magic, highlighting Jayson Tatum's big performance and the team's physical play. McKone feels the Celtics should handle the series more easily, while Beetle calls Tuesday’s Game 5 a must-win to take control. (6:37) Beetle says the Magic have controlled much of the first-round series, calling Game 4 a statement win for the Celtics after the frustrating Game 3 loss. He adds they could have swept the series, but now it’s become more difficult. The crew also breaks down Jaylen Brown’s performance over the last two games. (17:02) The guys discuss Bill Belichick’s CBS interview, focusing on his comments about not mentioning Robert Kraft in his book and the conflicting accounts of his departure from New England. They also touch on Jordon Hudson avoiding a question about how she and Belichick met. (29:14) The guys continue dissecting the CBS interview, zeroing in on Bill Belichick’s relationship with Jordon Hudson and questioning why both declined to answer how they met. They speculate on the secrecy and what it might suggest about the nature of their relationship.

Please Subscribe + Rate & Review KMJ’s Afternoon Drive with Philip Teresi & E. Curtis Johnson wherever you listen! --- KMJ’s Afternoon Drive with Philip Teresi & E. Curtis Johnson is available on the KMJNOW app, Apple Podcasts, Spotify, Amazon Music or wherever else you listen. --- Philip Teresi & E. Curtis Johnson – KMJ’s Afternoon Drive Weekdays 2-6 PM Pacific on News/Talk 580 & 105.9 KMJ DriveKMJ.com | Podcast | Facebook | X | Instagram --- Everything KMJ: kmjnow.com | Streaming | Podcasts | Facebook | X | Instagram See omnystudio.com/listener for privacy information.

Please Subscribe + Rate & Review KMJ’s Afternoon Drive with Philip Teresi & E. Curtis Johnson wherever you listen! --- KMJ’s Afternoon Drive with Philip Teresi & E. Curtis Johnson is available on the KMJNOW app, Apple Podcasts, Spotify, Amazon Music or wherever else you listen. --- Philip Teresi & E. Curtis Johnson – KMJ’s Afternoon Drive Weekdays 2-6 PM Pacific on News/Talk 580 & 105.9 KMJ DriveKMJ.com | Podcast | Facebook | X | Instagram --- Everything KMJ: kmjnow.com | Streaming | Podcasts | Facebook | X | Instagram See omnystudio.com/listener for privacy information.

Join this channel to get access to perks:https://www.youtube.com/channel/UCuvjQYKukKjVyhSVxQibgOw/joinGet 10% off your first month https://betterhelp.com/calumjohnsonStart investing safely with Public https://public.com/calumTry notion for free https://notion.com/calumjohnsonOur Merch drops soon! Be 1 of 50 to get EXCLUSIVE ACCESS: https://bit.ly/StayDelusional2 Follow Us!Instagram: https://www.instagram.com/calumjohnson1?igsh=MTdzbHI1b3c1b2dmag== Twitter https://x.com/calum_johnson9?s=21 Ashley Fox https://www.instagram.com/_ashleymfox?igsh=aW1paWVkcHdlNTB1Watch our episode with Donni Wiggins on how to get rich in 2025 https://youtu.be/GkxGipg1EEM00:00 Intro05:38 Most limitations are imaginary. 08:37 Imposter syndrome on Wall Street!13:01 Your environment determines your dreams 16:30 How wealthy people handle finance25:13 Difference in mindsets between the wealthy and the 99%30:35 How therapy changed her life35:59 I spent $100,000s to heal my trauma44:06 Allow yourself to heal 47:45 Don't just survive, thrive!52:20 How to create your roadmap for success59:56 How to be rich in this Trump economy1:05:00 Breakdown of how the stock market works1:10:00 Easiest way to choose stocks to buy 1:14:46 Full research process to pick the right stocks to invest in1:19:26 Red flags to check for before buying stocks 1:25:17 Stop panic selling1:32:40 Programming yourself for your first million 1:35:11 Separate your identity from your business 1:47:02 You can come back from anything About the video Today's guest is Ashley Fox. She's a financial educator, entrepreneur, and founder of Empify. In this episode, she shares her journey of overcoming self-doubt, healing through therapy, and reprogramming her mindset to unlock abundance. She also breaks down how to understand the stock market, start investing as a beginner and program yourself to make your first million. This is more than investment talk — it's a blueprint for healing and thriving.Public Disclosures: All investing involves the risk of loss, including loss of principal. Brokerage services for US-listed, registered securities, options and bonds in a self-directed account are offered by Public Investing, Inc., member FINRA & SIPC. Public Investing offers a High-Yield Cash Account where funds from this account are automatically deposited into partner banks where they earn interest and are eligible for FDIC insurance; Public Investing is not a bank. Cryptocurrency trading services are offered by Bakkt Crypto Solutions, LLC (NMLS ID 1890144), which is licensed to engage in virtual currency business activity by the NYSDFS. Cryptocurrency is highly speculative, involves a high degree of risk, and has the potential for loss of the entire amount of an investment. Cryptocurrency holdings are not protected by the FDIC or SIPC. A Bond Account is a self-directed brokerage account with Public Investing, member FINRA/SIPC. Deposits into this account are used to purchase 10 investment-grade and high-yield bonds. The 6%+ yield is the average, annualized yield to worst (YTW) across all ten bonds in the Bond Account, before fees, as of 12/13/2024. A bond's yield is a function of its market price, which can fluctuate; therefore, a bond's YTW is not “locked in” until the bond is purchased, and your yield at time of purchase may be different from the yield shown here. The “locked in” YTW is not guaranteed; you may receive less than the YTW of the bonds in the Bond Account if you sell any of the bonds before maturity or if the issuer defaults on the bond. Public Investing charges a markup on each bond trade. See our Fee Schedule. *Terms and Conditions apply.

Join Snaxton & Goose as they discuss current events, The Righteous Gemstones, and the life of a painfully average man. Don't forget to rate review and subscribe!

Question: What was the position of 19th century American Jews to the Civil War and Slavery? Answer: Complicated. Very complicated.Painfully and, in some ways, shamefully complicated, according to the historian Richard Kreitner. In his new book, Fear No Pharaoh, Kreitner explores the radically diverse positions that American Jews held toward slavery during the Civil War. He highlights 6 prominent Jewish figures including Judah Benjamin (a Confederate leader), Rabbi Morris Jacob Raphael (who justified slavery using Torah), David Einhorn (an abolitionist rabbi), Isaac Mayer Wise (who advised Jews to stay out of the conflict), August Bondy (who fought with John Brown), and Ernestine Rose (a radical feminist activist). Kreitner explains how American Jews, numbering around 150,000 by 1860, were - like the rest of the (dis)United States - deeply divided on slavery, with most influenced by regional issues that usurped the supposedly universalist religious ethic of their faith. 5 KEEN ON AMERICA TAKEAWAYS * American Jews were deeply divided on slavery and the Civil War, with most adopting the political views of their geographic region rather than having a unified "Jewish position."* The Jewish experience with slavery in Egypt (celebrated in the Passover tradition) created a complex dynamic for American Jews confronting American slavery, with some using it to oppose slavery while others justified the practice.* Jewish figures like Judah Benjamin rose to high positions in the Confederacy, while others like Rabbi David Einhorn were forced to flee for their anti-slavery activism.* Anti-Semitism was relatively subdued in the American South before the Civil War (as Black enslavement served as the primary social hierarchy), but increased during and after the war.* Figures like Ernestine Rose represented an intersection of Jewish identity, abolitionism, women's rights activism, and freethinking, highlighting the diverse ways American Jews engaged with 19th century social reform movements.Richard Kreitner is the author of Break It Up: Secession, Division, and the Secret History of America's Imperfect Union and Booked: A Traveler's Guide to Literary Locations Around the World. He has written for The New York Times, The Washington Post, The Boston Globe, The Nation, Slate, Raritan, The Baffler, and other publications. He lives in the Hudson Valley, New York. In his new Substack podcast, Think Back, Kreitner interview US historians about connections between the past and the present.Named as one of the "100 most connected men" by GQ magazine, Andrew Keen is amongst the world's best known broadcasters and commentators. In addition to presenting the daily KEEN ON show, he is the host of the long-running How To Fix Democracy interview series. He is also the author of four prescient books about digital technology: CULT OF THE AMATEUR, DIGITAL VERTIGO, THE INTERNET IS NOT THE ANSWER and HOW TO FIX THE FUTURE. Andrew lives in San Francisco, is married to Cassandra Knight, Google's VP of Litigation & Discovery, and has two grown children. This is a public episode. If you'd like to discuss this with other subscribers or get access to bonus episodes, visit keenon.substack.com/subscribe

Join this channel to get access to perks:https://www.youtube.com/channel/UCuvjQYKukKjVyhSVxQibgOw/joinSecure your business with $1000 off at Vanta: https://vanta.com/calum Start investing safely with Public: https://public.com/calumOur Merch drops soon! Be 1 of 50 to get EXCLUSIVE ACCESS: https://bit.ly/StayDelusional2  Follow Us!Instagram: https://www.instagram.com/calumjohnson1?igsh=MTdzbHI1b3c1b2dmag== Twitter https://x.com/calum_johnson9?s=21 Greg Isenberg: https://x.com/gregisenberg?s=21Watch our episode with Dom Ashburn on how to get 100k followers in 30 days using AI https://youtu.be/cai40ROkTYM00:00 Intro02:07 AI would change your life!03:10 What is an AI agent?08:07 2 business models to create with smart software 12:19 How you can build a $1M AI startup in 6 months. 17:55 How to use social media to build an AI business24:43 Why he dropped out of college 28:00 How to pick AI business ideas to work on. 36:39 Best time to launch your product 41:47 How to set up a business in 24 hours using AI agents (Live crash course) 55:29 How to turn your product into a movement 57:43 4 step playbook to build an audience in 20251:02:36 How to keep your users coming back 1:09:13 Why you MUST focus on the core loop1:11:10 The blueprint to partner with influencers. 1:14:57 How to build relationships with successful people About the videoToday's guest is Greg Isenberg — a startup entrepreneur, advisor, and CEO of Late Checkout, a company that builds and invests in internet communities. In this episode, he breaks down how AI is reshaping entrepreneurship and shares the exact playbook to build a $1M AI startup in just 6 months; from idea creation to distribution to customer retention — this is your blueprint for winning in 2025.Public Disclosures: All investing involves the risk of loss, including loss of principal. Brokerage services for US-listed, registered securities, options and bonds in a self-directed account are offered by Public Investing, Inc., member FINRA & SIPC. Public Investing offers a High-Yield Cash Account where funds from this account are automatically deposited into partner banks where they earn interest and are eligible for FDIC insurance; Public Investing is not a bank. Cryptocurrency trading services are offered by Bakkt Crypto Solutions, LLC (NMLS ID 1890144), which is licensed to engage in virtual currency business activity by the NYSDFS. Cryptocurrency is highly speculative, involves a high degree of risk, and has the potential for loss of the entire amount of an investment. Cryptocurrency holdings are not protected by the FDIC or SIPC. A Bond Account is a self-directed brokerage account with Public Investing, member FINRA/SIPC. Deposits into this account are used to purchase 10 investment-grade and high-yield bonds. The 6%+ yield is the average, annualized yield to worst (YTW) across all ten bonds in the Bond Account, before fees, as of 12/13/2024. A bond's yield is a function of its market price, which can fluctuate; therefore, a bond's YTW is not “locked in” until the bond is purchased, and your yield at time of purchase may be different from the yield shown here. The “locked in” YTW is not guaranteed; you may receive less than the YTW of the bonds in the Bond Account if you sell any of the bonds before maturity or if the issuer defaults on the bond. Public Investing charges a markup on each bond trade. See our Fee Schedule. *Terms and Conditions apply.

Dive into the world of choral music publishing with this episode featuring Jack Zaino from Alfred Music! Gain insights into Alfred's focus on serving public schools, teachers, students, and community choirs. Aspiring composers will discover essential advice on submitting music and building industry connections. Choral directors will find valuable tips for selecting the perfect repertoire. Explore the collaborative nature of music publishing and hear Jack's reflections on his mentor, Audrey Snyder. A must-listen for anyone involved in the choral music community.

The Texans are unlikely to add another starter FA. How do we feel about that? Are you ready to scrap the ‘Tuve in LF experiment? QOTD: Who or what were you painfully wrong about?

QOTD: Who or what were you painfully wrong about?

In hour one, Crowder wants Appel to know he doesn't respect his wardrobe decisions. Messi not selected for Argentina's national team - bad news for Inter Miami? Crowder pumps his chest over SEC dominance in the NCAA bracket. Plus, Will Manso joins the show to discuss the Heat's losing streak and big picture decisions that loom over the final 15 games of the season.

Today on the Matt Walsh Show, Gavin Newsom pretends to be moderate as he prepares for his inevitable 2028 presidential run. One of the astronauts trapped on the International Space Station confirms Elon Musk's claim that the Biden Administration wouldn't let Musk rescue them. We have another cultural appropriation controversy—haven't had one of those in a while. And a newly unearthed video shows that Representative Jasmine Crockett used to speak like a normal person of at least average intelligence before she was elected to Congress. Click here to join the member-exclusive portion of my show: https://bit.ly/4bEQDy6 Ep.1551 - - - DailyWire+: We're leading the charge again and launching a full-scale push for justice. Go to https://PardonDerek.com right now and sign the petition. Now is the time to join the fight. Watch the hit movies, documentaries, and series reshaping our culture. Go to https://dailywire.com/subscribe today. Get your Matt Walsh flannel here: https://bit.ly/3EbNwyj - - - Today's Sponsors: Grand Canyon University. - Find your purpose at Grand Canyon University. Visit https://gcu.edu today. Policygenius - Head to https://policygenius.com/WALSH to get your free life insurance quotes and see how much you could save. Renewal by Andersen - Text WALSH to 400-400 for a FREE consultation to save $379 off every window and $779 off every door. - - - Socials:  Follow on Twitter: https://bit.ly/3Rv1VeF Follow on Instagram: https://bit.ly/3KZC3oA Follow on Facebook: https://bit.ly/3eBKjiA Subscribe on YouTube: https://bit.ly/3RQp4rs

Arsenal trudged out of the City Ground with a 0-0 stalemate against Nottingham Forest—a result that felt like swallowing bitter medicine. Defensively resolute?Absolutely. Offensively forgettable? Painfully so. Arsenal's backline stood lively against Forest's physicality, but their attack sputtered like a car out of fuel, mounting on frustration as we count the days and weeks. The first half was a few memorable displays. Saliba, Gabriel, and Timber—operated like a fortified wall, snuffing out Chris Wood's threats. But up front, Merino's false-nine experiment worked...and worked, beyond recycled possession and hopeful crosses into a forest of defenders.  Bright spots flickered briefly: Tierney's introduction sparked width, forcing a sharp save from Sels, while Ødegaard's late blocked shot was simply would-be-victory. Yet Forest's low block proved impenetrable, reducing Arsenal to sideways passes and speculative efforts. The clean sheet? Laudable. The attacking inertia? We all know.   This wasn't a disaster, but we have a vacancy for hero. A point earned is fair. But we need to strive for more. Arguably. Tune in for byte-size breakdown.  Share this episode with fellow Gooners. Onwards, but are in it during these hard times. C'mon Arsenal!

[EP 25-076] James Woods tweeted: Two guys who have more money than they'll ever need are working for NO SALARY 16 hours a day to make the lives of hard-working Americans better. And all the Democrats do is bitch and moan. Former FBI assistant director Frank Figliuzzi just claimed that "If you voted for [Trump], you really need to question whether you're American anymore." This is a particularly chilling statement from a person appointed by Robert Mueller to head counterintelligence operations...I wish Elon Musk would use DOGE to find every anti-American and anti-Trump post on social media, so President Trump knows DIRECTLY who to fire.The Peter Principle, cooked up by Laurence J. Peter, is the brutal truth that in any hierarchy, people get promoted until they're bad at their jobs. You start off great, climb the ladder, and then—oops—you land in a role where you're completely out of your depth. Why? Because being good at one job doesn't mean you'll be good at the next one. It's like promoting a star quarterback to coach, only to realize he can't draw up a play to save his life. The idea was laid out in a 1969 book co-written by Peter and Raymond Hull. It was meant to be satire, but it hit a nerve because, let's face it, we've all worked for someone who proved the point. Now, it's a staple of management theory—and a perfect excuse for why your boss is the way they are. In short: promotions don't stop until incompetence does. Funny? Yes. Painfully accurate? Absolutely.Become a supporter of this podcast: https://www.spreaker.com/podcast/the-kevin-jackson-show--2896352/support.

Tiny microbes have a big impact on wine quality. Aria Hahn, CEO and co-founder of Koonkie, Inc., discusses the exciting work her bioinformatics organization is doing in the field of metagenomics. Hahn explains the differences between genetics, genomics, and metagenomics. She shares insights from a project studying yeast populations in British Columbia's Okanagan region, revealing the diversity and distinct clades found on wine grapes. The conversation also covers the broader applications of bioinformatics in agriculture, including regenerative farming, soil health, and potential bioprotectants against wine spoilage. Hahn underscores the impact of microbiome management on wine terroir and the potential of bioinformatics in understanding and improving winemaking processes. Resources:         201: Balance Hot Climate, High Sugar Wine with Green Grape Juice aka Verjus 243: Microbial Communities in the Grapevine 251: Vine SAP Analysis to Optimize Nutrition Aria Hahn – Google Scholar Aria Hahn – LinkedIn Koonkie Make Better Wines with Bioinformatics The Microcosmos - Discover the World of Genomics Apple App Whole genome sequencing of Canadian Saccharomyces cerevisiae strains isolated from spontaneous wine fermentations reveals a new Pacific West Coast Wine clade Vineyard Team Programs: Juan Nevarez Memorial Scholarship - Donate SIP Certified – Show your care for the people and planet   Sustainable Ag Expo – The premiere winegrowing event of the year Vineyard Team – Become a Member Get More Subscribe wherever you listen so you never miss an episode on the latest science and research with the Sustainable Winegrowing Podcast. Since 1994, Vineyard Team has been your resource for workshops and field demonstrations, research, and events dedicated to the stewardship of our natural resources. Learn more at www.vineyardteam.org.   Transcript [00:00:00] Beth Vukmanic: Tiny microbes have a big impact on wine quality. [00:00:09] Welcome to Sustainable Wine Growing with Vineyard Team, where we bring you the latest in science and research for the wine industry. I am Beth Vukmanic, Executive Director. [00:00:19] In today's podcast, Craig McMillan, Critical Resource Manager at Niner Wine Estates, with longtime SIP certified vineyard and the first ever SIP certified winery, speaks with Aria Hahn, CEO and co founder of Koonkie Inc. [00:00:35] She discusses the exciting work her bioinformatics organization is doing in the field of metagenomics. Hahn explains the differences between genetics, genomics, and metagenomics. [00:00:47] She shares insights from a project studying yeast populations in one of British Columbia's wine growing regions, revealing the diversity and distinct clades found on wine grapes. [00:00:58] The conversation also covers the broader applications. bioinformatics in agriculture, including regenerative farming, soil health, and potential bioprotectants against wine spoilage. [00:01:09] Hahn underscores the impact of microbiome management on wine terroir and the potential for bioinformatics in understanding and improving the winemaking process. [00:01:19] We know your customers are looking for sustainable wines. In a recent review of 30 studies, Customers reported a higher preference for eco label and social responsibility labels compared with nutrition labels. Achieving SIP certified gives you third party verification that your vineyard winery or wine has adopted and implemented stringent sustainable standards. Apply today at SIP certified. org. [00:01:46] Now let's listen [00:01:50] Craig Macmillan: Our guest today is Aria Hahn. She is the CEO and co founder of Koonkie, Inc., a bioinformatics organization, business, doing all kinds of exciting stuff. Thanks for being on the podcast, Aria. [00:02:02] Aria Hahn: Yeah, so excited to be here. Thanks for [00:02:04] Craig Macmillan: We're going to get into the thick of it But we were attracted to some work that you folks and your colleagues have done with bioinformatics and yeast, wild yeast. But I wanted to drop back. A little bit first to kind of give some context. All of this kind of comes under the umbrella of metagenomics, correct? [00:02:21] Aria Hahn: Yeah, absolutely. [00:02:22] Craig Macmillan: and what is metagenomics? [00:02:24] Aria Hahn: I'm going to take further step back and talk about genomics um, maybe the distinction between genomics and genetics. [00:02:32] So genetics is something I think most people kind of understand. They have this intuitive sense of it. um, that make up ourselves and all living creatures. But it actually turns out that in most organisms, and not bacteria, and we'll get there in a Most of your DNA is not in genes. It's in between genes. And so genomics is the study of genes and all of the things that are in between genes. So that's kind of the distinction between genetics and genomics. And then metagenomics is when we do that at the community level. [00:03:02] so you could do metagenomics of humans, but metagenomics refers to populations of bacteria, uh, microbes, archaea, viruses, things that you cannot see and I'll say interact with directly. [00:03:17] Craig Macmillan: And then bioinformatics is a subset or is a technique, is that correct? [00:03:23] Aria Hahn: Yeah, it's a technique, you know, it used to be even 10, 15 years ago that everyone kind of did their own bioinformatics. And so really what that means is when we sequence DNA or read that DNA, so it's only four letters, ATCs and Gs, we extract the DNA the sample is, could be the surface of grapes, uh, a human, anything. [00:03:42] Um, Then we put it on a sequencer. There's a bunch of different sequencing technologies right now. Um, But you end up with these like very gross files that aren't openable on regular computers and they're literally just ATCs and Gs. And so bioinformatics is the cross section of high performance computing and biology. And so we develop algorithms and processes and pipelines to really take those gross huge files of ATCs and Gs and make them human readable. make them interesting, figure out, you know, what are the genes that are there? Who is there? What are they doing? And who's doing what? [00:04:19] Craig Macmillan: Okay, and I think that's the important part here is you would take a sample from the environment. We'll talk about this one more in a second, but there are particular sequences that may be associated with a certain type of microbe or even a particular genus or particular species of microbe that can be detected. [00:04:39] Aria Hahn: Yes, absolutely. [00:04:40] So a genome is all of the DNA that makes up that organism. So you and I have distinct genomes, but of course, our genomes are going to be pretty similar to each other compared to a human genome, to a fish, to a plant, to a [00:04:55] Craig Macmillan: why the focus on microbes? [00:04:57] Aria Hahn: Yeah, that's a great question. It depends how philosophical You want to get You know, people are generally [00:05:02] familiar with the concept of like the Higgs boson particle. It's like the God particle that makes up everything and that's great and the physicists are gonna come for me. But when we think about our planet Earth, I always say like If there is a god particle on this planet that is alive and that we interact with, like, it's a microbe. [00:05:21] This is their planet. They were here long before us and they will surely be here long after us. So we think about microbes in terms of they are the destroyers of higher level populations. They keep us healthy. They make us I'm going to say it's a great example, but it wasn't a great thing. [00:05:40] So caveat that. But COVID was a great example about how this is not our planet where we had an of a virus in one location in a very particular place in the world. And all of a sudden it was across the planet. We are vectors for them. [00:05:58] You know, looking at those maps and showing the spread and how quickly it happened, I like to use that often in visual presentations to say, if you don't believe me, like, look at this. It's spread through us. [00:06:10] Craig Macmillan: Right. And I think also this gets to some other things we might talk about later on, but there are communities of microbes that are associated with certain macroorganisms. [00:06:23] Aria Hahn: uh, so are, they're everywhere, they're on your um, there's lots of research in the cosmetic industry that's looking at that. There was this crazy CEO years ago where he was I'm gonna slather this microbial laden cream on my skin and then I'm never gonna shower again and I'm not gonna smell. Not necessarily like my cup of tea, I love a good hot shower. But, you know, it can be there. The soil is the microbial diverse environment on the planet. , But your gut, like you, you as a human being, can't actually digest your food without those microbes. You can't get those vitamins and nutrients that you need without that community in your stomach. [00:07:03] Plants work the same We say charismatic macrofauna, eagles, whales, things that are very Um, They, they don't to, you the seaweed and the weeds and the grass and kind of everything in between. it's All supported by the microbiome, by these microbial communities. [00:07:20] Craig Macmillan: so let's talk about one microbiome in particular, and that would be populations of yeast that we find on wine grapes. [00:07:29] Aria Hahn: Yeah, yeah. So we've looked at yeast and bacteria and they're both cool. [00:07:34] Craig Macmillan: That is super cool. And so this one particular project where you looked at yeast on wine grapes in British Columbia Tell us a little bit about that project [00:07:41] Aria Hahn: there's, So I live in I'm, I'm right in the thick of, you know, BC wine country, which is a fantastic place to live, we were fortunate enough to work with the Wine Research Institute out of the University of British Columbia, Vivian Mease Day's group. them and They do very, very cool work, but they were trying to look at the yeast populations in wineries across the Okanagan region. [00:08:02] We know that the history of lots of commercial. Yeasts are actually from oak trees in Europe. So that's very cool. And what we wanted to see is how are the yeasts that are being used to produce wine in the Okanagan region distinct and similar to commercial yeasts and yeasts that have been characterized from across and so We did just that and we were actually able to sequence a whole bunch of yeast. And so, again, that's like reading the genome effectively there. so we found four distinct clades, um, in the Okanagan region. And a clade is they're related groups, and so it's not like you and you're a twin where you might have an identical, uh, genome to a twin. [00:08:50] It's more like you and your cousins and second cousins and second cousins twice removed and, you're, you're kind of vaguely related to each other. You kind of cluster over here, but you're not necessarily super We've kind of found four clades that the 75 yeast strains that we studied in that particular piece of work Really related to, then we looked at like what is different in their genomes. [00:09:12] So they're all the same species. That's the first thing to, to think about here. So just like you and I are the same species, they're all the same species, but just like you and I, we have different areas of, of specialties. Some people podcasters. Some people are, artists and scientists and, um, kind of everything in between. [00:09:33] And we need everybody. So, we're all the same species, but we have different specialties. And the yeasts work very similarly to that. [00:09:40] Craig Macmillan: all right, so this is interesting to me so You go out and you you said when you looked at 75 species of yeast or different types of yeast Those are ones that you, you found. It wasn't like you went in and said, I want to test for each of these 75. You got information, you got data in and said, Oh, look, here's 75 different types of [00:10:01] organism. Yes, that's a, that's a great Um, so, we And we uh, the ferment or the, the yeast skins and we extract the DNA and then we get rid of the great DNA, which could probably also be really cool, but we didn't look at it in this case. And kind of threw that into the and then said, okay, we're just going to focus on the Saccharomyces cerevisiae Latin term for a very common yeast strain, um, used in wine. And we said, we're going to look for it. [00:10:30] Aria Hahn: Then we found actually hundreds and . And then, um, and I didn't do this work, I don't do a lot of lab work myself, so, uh, this part is kind of the edge of my knowledge. But there is some ways to kind of do microsatellite clustering. And so you look, and you look for tiny differences in the genome, and you say, okay, maybe we found 500, but we actually want to look at ones that are distinct from one another. So we don't want to randomly pick 75, we want to pick 75. strains of this yeast that are different from one another. [00:11:01] And so you could use some lab techniques to make that happen. And then you take those hundreds and we say, these are the 75 that we know are different. We're going to dive deep into those 75 so that we can kind of get this breadth of genetic diversity from the region. [00:11:18] Craig Macmillan: And that was something I was thinking about. You mentioned you took samples from either fermenting wine or recently fermented wine or from skin material. How exactly is this collected so that you know that you're getting just [00:11:30] what you want? [00:11:31] Aria Hahn: Yeah. Painfully is the answer. So like when you do soil sampling, it's actually really And we tell people all the time sampling for yeast or microbes is not that complicated. I say every single time we talk to a client, I'm like, look, wash your hands with ethanol, you know, hand sanitizer, essentially between rinse your tools. And mainly you can't mess this up as long as you don't spit in the bag or bleed in the And I say this every time, and I will say one out of every few hundred samples is full of blood. Hands down like you always think we always the that and then hands down. We're like, this is full of And I think it's just like a matter of working in the field like people nick themselves They don't really realize that but really that kind of thing is really easy [00:12:15] When it comes to sampling a ferment that can also be fairly easy. [00:12:19] You have a lot of it You can kind and put it in a jar, but I will Um, Jay Marknack, who's done a lot of this work and developed a lot of these methods, he actually developed this method that is painstaking. Um, But you have like, he's got this method where he takes the grapes really carefully without touching them off of the and then washes just like very carefully with these like rinse solutions to really just get the microbes and yeast that are on that surface without touching it, without touching other surfaces. It's really just what was there in the field. And rinse all of that off. And you can imagine that's not that fun of a Um, And, and, and so, and it wasn't like he did it on his first try either. So he's now developed that we're copying and using, thank goodness. Uh, But it can be like that kind of painful where it's like washing individual grapes, collecting that rinse water, and then filtering that rinse water, like onto a physical filter, then extracting the DNA from that filter. [00:13:18] It's not fast. [00:13:19] Craig Macmillan: Nope, that's what I wanted to know. I've collected a lot of soil samples in my life for looking at soil microbiome. And you know, technique is everything. You know, contamination will mess you up pretty badly. [00:13:29] Aria Hahn: We had this one study I felt so bad, but they had collected these samples. They sent us the samples and we get the data back and it's, they're soil, they should be teeming with life, right? [00:13:38] And there's like one species basically in this thing, like there's a handful, but like one is dominating. So we go to them and we're like, what is going on here? And they're like, well, I don't see how that could have happened. , we've been storing these in a dark closet for a year. And we're like, that's why. You are studying bottle effects right here. And they're like, oh, we thought it'd be fine because it was dark and cool. And we're like, yeah, but it's not open to the air, and it doesn't have the plants and animals and bugs. You grew one guy. [00:14:07] Craig Macmillan: Yeah. We've been talking about bacteria, or the yeast. Are there other types of organisms, microorganisms, that you can use this technique with? [00:14:14] Aria Hahn: Totally. So you can use this technique on basically anything that's alive. So you could target viruses, uh, not something we've done on wineries, but could absolutely do it. You can target, , archaea, which are very similar to bacteria in that there are a single cell. But they are similar to eukaryotes. [00:14:32] So things that are bigger, um, like us, like mammals, like fish, Uh, but they are kind of small and invisible, , to the naked eye like bacteria. So those, we can, we can do that all the way up to, any animal that we can see, feel, touch, , and kind of anything in between. So it's a really powerful technique. As long as it has DNA, we can make this work. [00:14:53] Craig Macmillan: So you found these 75 types? of yeast organisms, but they fell into groups, they fell into clades. And I thought that was one of the most interesting things about this. Can you tell us a little bit about the natural history of behind these clades and kind of what that means? [00:15:09] Aria Hahn: We found these 75 different strains and they did group into four clades. So four kind of groups of more or less related organisms. So you can think of them as like clustering based on similarity. [00:15:22] The first one was one that is well known and well studied. So that's wine and European. And so those strains are more similar to these that we see in wines out of Europe and commercial strains. [00:15:35] And then the second clade we saw was the trans pacific oak. So a lot of wine yeast are very closely related to yeast that are found on oak trees. And so actually think that, , the original, , European wine yeast strains from, you know, the 1800s are from Mediterranean oak trees. And so it's not uncommon that we see these strains related to oak. [00:15:59] So that was the trans pacific oak. Then we see another group or clade that we called beer one mixed origin. And so we saw similarities to known previously studied yeast strains that are related to beer, sake, so other kind of fermented drinks. also kind of expected. [00:16:18] And then what was really exciting is that we found a new clade that we've designated the Pacific West Coast wine clade. it's always neat when you get to discover something new, of course. And so it has high nucleotide diversity. And so what that means is that even within this clade we do see a lot of genetic diversity kind of in there. [00:16:38] And what we do know is that that whole clade shares a lot of characteristics with wild North American oak strains, but, and this is kind of where like it all kind of comes full circle, but we also see that it has gene flow from the wine European and Ecuadorian clades. It can mean a couple of things. So it could mean that There is just so much selective pressure when you're, when you're trying to make good wine that these genes that are found in European wine strains, commercial wine strains, they're present in Saccharomyces cerevisiae in general, but then when we try to make good wine, we select for strains that have these, genes, , that we know produce good wine, because they produce good wine everywhere. [00:17:27] And so it could just be this process of natural selection. It also could be that most wineries , are not purists. It's not that. never in their history have other wine strains visited their their vineyards. They might have tried a commercial strain. They have wine from others, you know, people track things in, animals track things in. And so it could just be that there is this gene flow, quite literally from, from Europe, from these wine strains that just kind of comes into our population here in the Pacific West Coast. [00:18:00] And so there's kind of these, these two ways that we could have got these things, We do have some evidence to suggest that they were actually transferred in. [00:18:07] So it's called horizontal gene transfer. And my go to example on how horizontal gene transfer works is always , The Matrix, like the movie with Keanu Reeves. But what I've also learned is that if you talk to people that are like younger than me, they don't know that movie anymore, so this only lands with like a certain age of [00:18:23] Craig Macmillan: Right, I know, I know, [00:18:24] Aria Hahn: You know The Matrix where they like plug in and then they have all these new skills? [00:18:28] Bacteria can kind of do that, where you can just take genes from, , a relative, has to be like kind of closely related, and we take them and then we just put it into their genome, and in many cases, not all, but many, they're able to just kind of start making use of those genes right away. [00:18:43] And so that's horizontal gene transfer, which is pretty cool, because for us, the second that sperm hits the egg, that's it. That's all your genes. You're not getting more. You're not losing more. Like you're, you're set. But bacteria are more fluid. [00:18:57] So there is this cool thing called the wine circle, and it's a cluster of five genes that are associated with making commercial wine. [00:19:05] And we do think because we see this wine circle and these particular five genes in so many wine strains, and because of their location and a whole bunch of other kind of genomic characteristics of them, Um, we think that they are horizontally transferred. And so we do see this wine circle of these five genes in the majority of this new clade of British Columbia strains. [00:19:33] Craig Macmillan: So just talking about moving things around the world, you said like people have things on their bodies and whatnot. I, I was fascinated by the Ecuadorian group. And is that literally like it was growing on plants in Ecuador, kind of native to that area that is found its way up the West coast of North America. [00:19:53] Aria Hahn: that's really what we thought happened. I know it is amazing, right? Like does the amount and transfer and you know how you go through the airport and they're like, you and It's like the end of the world. It's like I get it because we don't want to like do that on purpose, but also the ecuadorian yeast like it's coming up here [00:20:12] Craig Macmillan: right, right. Exactly. [00:20:14] So what I think is of interest to winemakers, and also has potential beyond that that I'll ask you about winemakers are looking for increased complexity in their wines, and they're also looking for a sense of place. And I'm really happy to hear more and more people talking about terroir, not just in terms of rocks, but in terms of the whole picture. [00:20:33] The soil microbiome, the practices that are done, as well as light and climate and all those kinds of things. What are some of the things about what you found that indicate or that suggest a uniqueness to that Okanagan area that may make it stand out as different than other locations? How does this translate into sense of place? [00:20:54] Aria Hahn: That's a fantastic question. I'm going to give two answers first on the east side. We see that many of that nucleate. don't have all five of those wine circle genes. And so we see a lot of British Columbian strains have that, but there's this whole clade of these natural yeast used in wine that don't have all five of those. [00:21:17] So then you just have different genes to work with. And since you have different genes to work with, it's not just those genes, but it's all of the genes, and it's the rate that those specific strains are able to break things down. [00:21:28] You do get this added complexity when you're not using a standard commercial yeast. You just have this bigger variety of genes to choose from, and That's going to make the flavors more specific, and different. [00:21:44] It also introduces a certain, the disadvantage of using these is that, you know, they are gonna vary year to year, month to month. Uh, Potentially, and, and so you might get really, really amazing results one year and not the next year, and understanding why, why that might be is a whole exercise in and of itself, probably doable, but it's really exciting to think that these yeasts that are there naturally , they just have that genetic diversity and they want to live in these diverse communities, and so you are going to get that difference and terroir. [00:22:16] The other piece that was really exciting and was a different piece of work, but very similar groups and very similar, , samples, was looking at the microbiome, so the bacteria on the grapes. And we kind of found two things, and so there is some literature that shows if you look at a single farm, a single vineyard, and you look at different red varietals of grapes, you see actually a fairly similar microbiome signature on all of the different varietals. [00:22:46] Okay, but if we look at three distinct vineyards that are all within , one kilometer radius of one another. So they're very close. They have the same rock, to your point. They have the same weather. They have the same climate chaos happening, [00:23:01] but they're managed differently. We actually see very, very distinct signatures on all three that persists year after year. So we looked at two years, , this was again, Jay Martinek's work, , and we see that each one of those, even though it's the same varietal of grape, it is more similar to itself, year over year, than among the three farms. and and that's very interesting because what that suggests is Exactly what you're saying. [00:23:29] It's not the rock. It's not the climate that's driving the microbiome there. It's actually the practices of that vineyard that are changing that. And to me, that's so powerful, because what that means is that there's so much of that craft and art in the management of the vineyard that's then going to go and affect the terroir. , I know that's not the yeast answer, but that's the bacteria answer, and it's like, the power's in your hands. [00:23:54] Craig Macmillan: I'm on the Central Coast of California, and we've had some very hot vintages in this last , 2024 season. We had, and it was 2022 as well, we had these really hot stretches of over 100 Fahrenheit. Not very friendly to yeast in general. [00:24:09] Probably friendly to some, but not to others. And I had conversations with winemakers along the lines of like, could you even do a natural fermentation this year, a native fermentation? Are they there anymore? Or have they been selected against due to the heat? And I now have a total reset of the microbiome, the microflora in my world. this is the kind of thing that bioinformatics would be able to determine. [00:24:34] Aria Hahn: yeah, for sure. So we love that. We love when we get the baseline. We're like, show us your year that you were like, this is my typical year. This is my regular year. We'll live for that because as soon as you have the baseline, then we can go and answer those questions. So we can say, okay, great. We know what your baseline is when you typical year. [00:24:52] Now you have this heat wave that comes in. , Let's go and look. Let's go see who's survived. And I know I anthropomorphize all of these things a ton, but it really is, like, who's there, right? , is it the same bug, but very decreased? Are we getting different E strains coming in? are we seeing less overall diversity? [00:25:13] Do we see the same diversity, but Their population is a quarter of the size, and how does that affect the dynamics? Like, what do we see? And bioinformatics can absolutely absolutely answer these questions. And that can be really powerful. [00:25:26] Craig Macmillan: In my research I didn't pick up on this Can bioinformatics put a quantity on things? Can you quantify the relative size of these different populations? [00:25:34] Aria Hahn: We can, yes. So, you have to use some kind of special techniques. There's a couple of main ways we do them. One is called qPCR, so quantitative PCR. And so we literally take the DNA and we can count the copies of it in a very quantitative way. That's straightforward, pretty inexpensive. [00:25:52] Another way we can do it is a little bit more sophisticated, , but you don't have to know what you're looking for. So with quantitative PCR, we have to know, like, we want to go count saccharomyces cerevisiae. But if we don't know all of the microbes that are there, all of these that are there, then we can't go and target it with qPCR. So then what we have to do is use a spiken. the concept is pretty simple. You put a known quantity of a piece of DNA that we would not expect to appear in nature. And then when we sequence it, we know how many we got back. So if we know we put in a hundred copies of it and we get 200 copies back, now we have a pretty good idea of like, everything there was, sequenced twice or if we get 50 copies back, we're like, okay, well, however many we have, we're going to double that and we have a good idea and we do do this in like a little bit more sophisticated way where we put in like a whole bunch at different quantities so we can double check our math and make sure that it's all good. [00:26:49] But that's the concept is with a spike in so you can do it quantitatively. [00:26:53] Craig Macmillan: Talking about all the things that are out there, there's a lot of interest right now in bioprotectants for fermentation, where you introduce non fermentative yeast, and they kind of take up the ecological niche against foliage organisms, and then you can add a Cerevisiae strain to do that, to do your fermentation. [00:27:10] Would you be able to pick up those other genus, of yeast in a bioinformatic way and gives us a sense of what else is out there. [00:27:18] Aria Hahn: Yeah, for sure. So we sequence the whole community and then we kind of in a. Like a puzzle. I'm going to put together the individual genomes of everyone who's there. And so we can look at not just the targets, but the unknowns as well. And so often, especially in soils, what we get is sometimes up to 80 percent of the genomes that we're able to recover from that sample are totally novel. [00:27:43] So they're new to science. It's really exciting. and we hate it. We love it and we hate it. So, we love it because it's really fun. You, you discover these new species of bacteria, of yeast, or these new strains, and, and you get to name them. You don't have to name them after yourself anymore, you have to name them about the place that they're there. Which is a totally logical thing. But, would have been fun. , [00:28:06] So we get to name these things, it's really exciting. [00:28:09] But it also means it's so much work. Because now you have this genome that's so new. And so now you're trying to figure out. What are all the genes? Do we know the genes it has, but just not quite the way that they're arranged? Do we not know what many of these genes do? And if we don't know what these genes do, like what kind of uncertainty and questions does that bring up? And so it can be really exciting, that discovery phase, and also quite overwhelming, honestly. [00:28:36] Craig Macmillan: what other applications might there be for bioinformatics in wineries or in vineyards? [00:28:41] Aria Hahn: Yeah, that's fantastic. So definitely monitoring. You know, regenerative farming is a really big thing right now. how can we introduce additional species, cover crops, , you know, planting additional or different plants in between. Like, what can we do to really increase the soil health, sequester carbon, the biodiversity of the soil, of the land, and how does that affect it? So we can monitor all those things with environmental DNA or eDNA. [00:29:09] One thing that we've been thinking about a lot is this concept of smoke taint, which I think has kind of affected the whole west coast of North America. [00:29:18] Are there microbial treatments that can kind of mitigate smoke taint, , can we feed bacteria, the bacteria that we know [00:29:29] can kind of break down those volatile phenols that cause the smoke taint. Get them to kind of break that down first before we make the wine. Like we're kind of looking at applications like that. [00:29:40] Obviously those are, I would say further out in terms of technological development biodiversity, which we can absolutely go and do today. , but there's interest in that smoke taint. Application, and we're really interested in that. [00:29:52] Then there's also kind of everything in between. So can we the harvest? Can we increase the quality of the grape? Can we help with years that are dry? Can we help with years that are wet or cold or hot? as we, kind of committed to a certain number of effects of climate change, we have to start thinking creatively. [00:30:14] I was on this call with an unrelated company. They wanted to do similar things but in the mining space, in the reclamation space. And I don't know how it happened, but I was on this call with this man. It was his last day before it was dark where he was. He's in Quebec. He's three hours ahead. [00:30:29] , You know, it was winter. So it's very, the mood was very, like, dark and somber, and this was his final call of his final day of work. And he was so hopeful about microbes, and he spent his career working with them. And just before he signed off the call, he says, I hope microbes save us all. [00:30:50] And then he kills the call. And, and, for, the next few years, I titled every single talk I did, Microbes may save us all because I just, the weight of that conversation was so big and I know that's not what we're talking about here in terms of [00:31:08] smoke taint, but I do think, you know, to bring us full circle to this like omnipresent godlike presence of microbes that there's something to that idea in that I think that they have this potential to save us from ourselves. If we can learn more, [00:31:25] Craig Macmillan: I think what we're talking about is bioremediation and the potential there. And bioremediation would work by identifying an organism that's going to play a certain role and then actually introducing it into the environment. For instance, like introducing it to wine that may have smoke taint, for instance. [00:31:40] Aria Hahn: , so there's a three main approaches to that. [00:31:42] So the first is exactly what we're talking about. You introduce a micro that we know and you, and you put it in there. The main challenge of that is this, this word we call engraftment. We actually steal that word from organ transplants. So, when you put in a new heart, not that I know anything about heart transplants, but when you put in a new heart, you have to engraft it. [00:32:01] And so people need to be on immunosuppressants, is my understanding, to make that heart transplant like stick in their body, have their body accept it. Kind of the same challenge when you introduce a microbe into an open, wild environment where you need that new species to engraft in that community. If you can't do that, you just have to keep adding it. [00:32:21] You have to keep adding it, keep adding it, it's time consuming, it's expensive, all of these things. So engraffing is still a challenge in that field. But that is one way. [00:32:29] The second way is to bioengineer. And so the concept here is that you take species or strains that are naturally occurring, so they do well in that environment, and you change something in their genetics and then reintroduce that. It does get around the concept of, [00:32:50] of engraftment in theory. The major issue with it is, there's not a lot of people or companies that feel ready, I think, to take a biologically engineered synthetic genome and introduce it into the environment en masse. We just don't understand the risks of it, or, or not, we don't know, but I think that's the point, is that we don't know, and so people are a little bit like, Maybe we're not quite there yet. [00:33:19] And then the third way is to say, I'm going to look at who's already there. And I'm going to understand what they like to eat and what their competitors like to eat and I'm going to try to starve their competitors [00:33:31] and really feed the ones who have the capacity to degrade those volatile phenols. I'm going to like try to get their population to do super well and thrive. and and try to kind of starve out and make the populations that can't do the job that I want lesser and less prevalent in the community. [00:33:51] And that approach I think is kind of one of my favorites where we understand and then we put some selective pressures. So this could be adding more nitrogen, adding different carbon sources. [00:34:01] It could be watering less to create a more aerobic environment. It could be you know, kind of drowning them to create an anaerobic environment. It's kind of those bigger controls that we have working with the microbes that are already there. [00:34:17] Craig Macmillan: Yeah, in the same way that we're not afraid to play with plant communities in agricultural systems, with cover cropping or intercropping or anything like that, same kind of idea, where maybe I plant something that I think will out compete a weed. [00:34:28] Same kind of idea. And we're pretty comfortable with that. [00:34:32] And also things will have a way of finding their stasis, finding their, their It's just getting it kind of pushed in the right direction. I think that he's super, super cool. [00:34:44] A lot of interest and work in the soil microbiome in terms of soil health. We mentioned regenerative agriculture. I have put my toe in that, in, in my professional world extremely difficult, extremely confusing, lots of holes you know, and, and trying to find markers or metrics has been. challenging for instance, I was trying to figure out how healthy some soils were. It, healthy in quotes, and I wanted to do analysis of respiration. And this very good soil ecologist said, well, that tells you how many folks are in the room, but it doesn't tell you what they're doing or who they are. [00:35:21] And I was like, that's a really good Point I could have a lot of respiration from organisms. I don't want and I wouldn't know what was who and who was what? What world can bioinformatics play in that [00:35:33] Aria Hahn: , that's a great question. So I would say it's the opposite in general, without the spike ins and kind of specific things, what? we can tell pretty inexpensively, 50 to 100 a sample, is basically who's in the room and in what relative abundance. So it is come down a lot in price. It doesn't tell us a lot about their genetic capability. [00:35:55] So if we know them because they've been previously studied, then we can say like, oh, yeah, these guys are known to do X, Y, and Z. [00:36:02] If we don't know them, for that kind of price point in those methods, we're kind of just like, yeah, we know their names. But that's it. [00:36:08] Then we can do kind of a deeper dive, , to a different type of sequencing called whole genome sequencing. And you get the whole genome. And so there we can actually say not only who they are, but what they're doing. Or what they have the ability to do. And so that's where the limit of DNA is, is that it can tell us the potential. They can potentially do this, but it doesn't actually tell us if they're choosing to do that, so to speak. [00:36:33] There are other techniques that are very related. Metatranscriptomics, it's looking at the RNA, and you could do metabolomics. So you can actually look at the metabolites that they're producing, and then it tells you what they actually did. But we often can start at that base layer of DNA. and build up. So those questions we can answer. [00:36:51] And I think you're right about there are a lot of holes and it's confusing and it's complex. And we say this to clients all the time, like, if you know way to solve a problem, do that. Biology is messy. [00:37:03] But if you don't, like let's look at biology and let's enjoy the mess , there's a lot of beauty in that mess. And that's one of the things we've actually loved about interacting with wineries they are incredibly scientifically minded folks. They're data driven, the amount of innovation and technology they're using. never fails to impress, but you also get that love of the art and the craft from them. We love that. We see art and science as like in a circular spectrum. And so we love when, our clients in the, in the wine start talking to us about kind of their secret sauce and the things that they've tried and how, and they always get a little bit nervous. [00:37:49] And they would, if they always kind of start, they were like, you know what else I do? And we're like, tell us. And then they tell us something and they're like, we just know from experience. Experience that this works that this changes the ferment, but we don't have any evidence for that And and I think they're worried we're gonna judge them but we're like no that is like their science is all way of knowing but [00:38:09] my friend says art is science and love and and I love that idea that is something that's been really really fun about working with wineries and vineyards is they kind of get that they're like, yeah, this is the love piece here [00:38:22] Craig Macmillan: That's cool I think there's beauty in the mess. I might adopt that if you don't mind I mean, I may use that for some of my own stuff. I think that's great What is one thing you would tell growers or wineries, , [00:38:35] Aria Hahn: their choices are directly impacting the microbiome, so that's the bacteria and the yeast And that that is going to affect the terroir, the complexity, the quality of the wine, and it is knowable. [00:38:50] Craig Macmillan: there we go. And we also know that some of the things that we do may affect that and that is part of what makes us special. Where can people find out more about you? [00:38:58] Aria Hahn: We have a website, it is koonke. com, K O O N K I E dot com. can also look me up, Aria Hahn, , and on Google Scholar, the internet, I feel like I'm very findable. [00:39:10] Craig Macmillan: Yeah, you are very findable and we will have a lot of links and other things on the show page. So please check that out. Really fascinating stuff going even beyond this. I want to thank you for being on the podcast. [00:39:21] This has been a great conversation. [00:39:22] Aria Hahn: Yeah, thanks for having me. Super fun. [00:39:25] Craig Macmillan: So our guest today was Aria Hahn. She is CEO and co founder of Koonkie, a bioinformatics company, and is doing some really fascinating stuff, not only around yeast, but lots of other topics. [00:39:35] And I just got lost down the rabbit hole when I took a look at that website, all the different things you folks have been involved in, and it was really fun. [00:39:48] Beth Vukmanic: Thank you for listening. [00:39:49] Today's podcast was brought to you by Sunridge. For over 45 years, Sunridge nurseries has supplied premium quality grapevines. to grape growers worldwide. A pioneer in the industry with a focus on clean quality vines and personalized dedication to their partnered growers has led them to be the largest, most well respected grapevine nursery in the United States. Sunridge Nurseries continues to lead the industry having undergone several expansions to their modern state of the art facilities and is the first and only grapevine nursery to have implemented the most advanced greenhouse Horticulture water treatment technology in North America. [00:40:26] Make sure you check out the show notes for links to Aria, an article titled, make better wines with bioinformatics plus sustainable wine growing podcast episodes, 201 balance hot climate, high sugar wine with green grape juice, 243 microbial communities in the grapevine. And 251 vine sap analysis to optimize nutrition. [00:40:50] If you liked the show, do us a big favor by sharing it with a friend, subscribing and leaving us a review. You can find all of the podcasts at vineyardteam.org/podcast, and you can reach us at podcast at vineyardteam. org until next time, this is sustainable wine growing with the vineyard team.   Nearly perfect transcription by Descript

Find out more about Rep. Josey Garcia here.  all the podcasts.our FB pageour website.our Youtube channel.our patreon.our Twitter.our Insta.Our TikTok.Our Reddit.Our newsletter signupCome see our live show! October 11-13th, 2024 at the Scottish Rite Theater in Austin, Texas. .

We revisit the movie where Rachel McAdams and Jason Bateman are the comedy duo of the century. Zach, Kelsey, and Garrick lust over the witty jokes and brilliant performances. Learn more about your ad choices. Visit megaphone.fm/adchoices

Join me with my guest Ryan Tuckwood, Australia's top sales coach, who highlights the importance of skill development in sales and job interviews, emphasizing that being yourself isn't enough to reach your goals. Learn more about cold calling, building relationships, and personal branding in today's digital age. Don't miss out on Ryan's powerful reminder to take risks and roll the dice—sometimes, that's exactly what you need to unlock your full potential. Episode Highlights: 00:00 - Episode trailer 02:33 - Client Success Stories 05:03 - Overcoming Introversion 07:18 - The 10-step Negotiation Ladder 10:04 - Making a Strong First Impression 12:53 - How to Shift Emotions Positively in Interviews 19:15 - Understanding Personality Types 27:39 - Decision-Making Strategies 30:44 - Barack Obama's 60% Rule 33:38 - Understanding Worst-Case Scenarios 36:27 - How Skills Build True Confidence 39:39 - Crafting the Perfect Cold Call 45:36 - Exploring the Psychology of Authority 52:23 - Using Subtle Language for Authority 57:08 - The Power of Preempting Scripts 01:05:17 - The Two Words You Don't Want on Your Deathbed About my guest: Ryan Tuckwood is a renowned sales coach and the co-founder of SWISH Sales Coaching, widely recognized as the No. 1 ethical sales coach globally. Once a struggling, reluctant sales professional with just 31 cents to his name, Ryan turned his life around through relentless study and a deep understanding of sales as a process. He has since coached nearly 6,000 businesses and individuals worldwide, helping them generate over $1 billion in new sales revenue. Ryan's clients include top companies like Mercedes-Benz, AMEX, and Westpac. His expertise has earned him the opportunity to share the stage with figures such as Grant Cardone and Jordan Belfort. In 2018, Ryan and his co-founder Jack Corbett made history on Channel Ten's Shark Tank, securing the support of three Australian entrepreneurial heavyweights—Dr. Glen Richards, Steve Baxter, and Andrew Banks—who are now shareholders and advisors to SWISH Sales Coaching. Connect with Ryan Website: https://www.swishsalescoaching.com/ Instagram: https://www.instagram.com/ryantuckwoodofficial/ YouTube: https://www.youtube.com/channel/UCL93CDfMKRqglmU_8ey_YbA Become a globally recognized Trainer and Speaker in just 90 days! https://www.speaktoleadblueprint.com/speaktoleadblueprint Want to manage your life the same way that helped me make $1 million by 28 and travel the world at the same time? Grab my FREE one-page Life By Design productivity planner below ⁠https://planner.morgantnelson.com/optinplanner Join the Dream Out Loud Facebook Community https://bit.ly/49QXClW Follow me on Instagram here https://www.Instagram.com/ Subscribe to my YouTube channel https://www.youtube.com/@morgantnelson

Scott be like

“Family Proclamations” is the current podcast by Blair Hodges, host of Fireside. Enjoy this sample episode and be sure to subscribe directly to Family Proclamations now, because this episode will fall out of the Fireside feed next month!

Paul Scheer is an award-winning actor, screenwriter, comedian and podcast host of How Did This Get Made? So you'd think his most interesting life stories would be about his audition for Saturday Night Live or how he came up through improv comedy to star in shows like The League and Black Monday. Those are interesting stories, but in his new memoir, Paul gives center stage to family stories. Growing up with a loving mom and dad who divorced, the tyranny of an abusive stepfather, and what it's like to become a marriage partner and a parent in the shadow of traumatic experiences.  About the Guest Paul Scheer is author of the New York Times best-selling memoir, Joyful Recollections of Trauma. He's a comedian and a Screen Actors Guild Award-winning actor from shows like The League, Veep, Fresh Off the Boat, and Black Monday. He's a podcaster of the smash-hit How Did This Get Made and also Unspooled with his co-host Amy Nicholson. He lives in LA with his partner June Diane Raphael and their two kids.  Full transcript available at familyproclamations.org. 

In this episode, transformational coach Emile shares the hidden challenges faced by high achievers, sharing his own journey from the modeling world to realizing the importance of self-acceptance. Discover how the quest for external validation can lead to burnout and offers practical strategies for fulfillment, including setting boundaries, celebrating small wins, and creating personalized routines. Emile also shares grounding practices like meditation and tapping to help high performers balance ambition with self-care and break free from self-sabotage. Episode Highlights: 00:00 - Episode trailer 02:47 - The Disconnect Between External Success and Internal Fulfillment 07:22 - The High Achiever Syndrome 11:07 - Celebrating the Simple Wins 14:56 - Understanding Where You're Creating From 17:30 - How Who You Believe You Are Shapes Your Actions 21:39 - Blind Spots of High Achievers 25:30 - Slowing Down to Speed Up 34:35 - Creating a Personalized Morning Routine 38:13 - Mastering Your Nighttime Routine 42:59 - Exploring Self-Sabotage Patterns in High Performers 46:05 - Why We Self-Sabotage 47:54 - The Emotional Connection to Money 52:35 - The Impact of Early Unacknowledged Dreams on Career Success About my guest: Emile Steenveld is a Human Behavior Expert and Transformative Guide, assisting countless individuals in their journey toward self-fulfillment and self-actualization. He leads his clients through deep internal shifts, addressing and mending long-held limiting beliefs and dismantling internal barriers that prevent them from realizing their full potential and success. His mission is to facilitate profound personal and professional transformation. He is dedicated to guiding individuals, particularly high achievers and entrepreneurs, on a journey of self-discovery, reconnection, and authentic expression. He recognizes the contemporary challenges faced by ambitious individuals who relentlessly pursue success, often at the expense of personal well-being and genuine relationships. His goal is to provide a comprehensive methodology that empowers them to overcome burnout, embrace self-acceptance, and excel both personally and professionally. Connect with Emile  Website: https://www.emilesteenveld.com/ Instagram: https://www.instagram.com/emilesteenveld/# YouTube: https://www.youtube.com/channel/UC0_RUx6ziLfhAw_6o1YVoXw Become a globally recognized Trainer and Speaker in just 90 days! https://www.speaktoleadblueprint.com/speaktoleadblueprint Want to manage your life the same way that helped me make $1 million by 28 and travel the world at the same time? Grab my FREE one-page Life By Design productivity planner below ⁠https://planner.morgantnelson.com/optinplanner Join the Dream Out Loud Facebook Community https://bit.ly/49QXClW Follow me on Instagram here https://www.Instagram.com/ Subscribe to my YouTube channel https://www.youtube.com/@morgantnelson