Podcasts about Polymerase chain reaction

Send us a textSLAS Scientific Director Lesley Mathews, Ph. D., speaks with several winners of our Tony B. Travel Award who presented at the SLAS Europe 2024 Conference and Exhibition.Congratulations to our winners!(Listed in interview order)Shitanshu Devrani, Ph.D. (University of Gothenburg)An Automated High-Throughput Platform for AI-Guided Development of Nanopore Membrane Biosensors and Real-time Edge Processing, Demonstrated with Wilson's Disease Sample VerificationLeticia Manen, Ph.D. (Bellvitge Biomedical Research Institute)Harnessing Drug Metabolites In Precision Medicine: Deeping into antineoplastic's major metabolites polypharmacologyJoel Sanchez Barea, Ph.D. Candidate (Incheon National University)Multiplexed detection of single-point mutations in human serum by integrating Surface Enhanced Raman Scattering with Polymerase Chain Reaction.What is the Tony B. Travel Award?This annual travel grant program provides funding for the SLAS International Conference and Exhibition and the SLAS Europe Conference and Exhibition for students, graduate students, post-doctoral associates, and junior faculty with less than four years in their first academic appointments.  Visit our Student Resources to learn more about these awards.Stay connected with SLASOnline at www.slas.orgFacebookTwitter @SLAS_OrgLinkedInInstagram @slas_orgYouTubeSLAS (Society for Laboratory Automation and Screening) is an international professional society of academic, industry and government life sciences researchers and the developers and providers of laboratory automation technology. The SLAS mission is to bring together researchers in academia, industry and government to advance life sciences discovery and technology via education, knowledge exchange and global community building.  For more information about SLAS, visit www.slas.org.SLAS publishes two peer-reviewed and MEDLINE-indexed scientific journals, SLAS Discovery and SLAS Technology. For more information about SLAS and its journals, visit slas.orgUpcoming SLAS Events: SLAS 2024 Data Sciences and AI Symposium November 12-13, 2024 Cambridge, MA, USA SLAS2025 International Conference and Exhibition January 25-29, 2025 San Diego, CA, USA SLAS Europe 2025 Conference and Exhibition 20-22 May 2025 Hamburg, Germany View the full events calendar

PodChatLive 145: Why can't Ian pronounce Dermatoscopy, treating plantar warts, and orthotic deformation testing Contact us: getinvolved@podchatlive.com Links from today's episode: Nike CEO's LinkedIn profile Reliability and Validity of the Orthotic Deformation Test Cantharidin (1%), podophyllin (5%), and salicylic acid (30%) formulation in recalcitrant plantar warts Diagnostic Accuracy of Dermatoscopy Versus Microbiological Culture and Polymerase Chain Reaction in the Diagnosis of Onychomycosis

Our hosts, Dom and John, are excited to welcome special guest, Carrie Vyhlidal, to discuss the topic of the 80th episode of “The Weekly Bioanalysis” podcast – The Rapid Evolution of PCR Assays. PCR (or Polymerase Chain Reaction) is like a copying machine for DNA. Scientists have found ways to measure the copies being made throughout the reactions, which is where they get their real-time, quantitative PCR (or qPCR). Carrie does a wonderful job of explaining not only why these advancements in the study of PCRs are so amazing – but also why they play (and will continue to play) such an important part in bioanalysis.“The Weekly Bioanalysis” is a podcast dedicated to discussing bioanalytical news, tools and services related to the pharmaceutical, biopharmaceutical and biomarker industries. Every month, KCAS Bio will bring you another 60 minutes (or so) of friendly banter between our two finest Senior Scientific Advisors as they chat over coffee and discuss what they've learned about the bioanalytical world the past couple of weeks. “The Weekly Bioanalysis” is brought to you by KCAS Bio.KCAS Bio is a progressive growing contract research organization of well over 250 talented and dedicated individuals with growing operations in Kansas City, Doylestown, PA, and Lyon, France, where we are committed to serving our clients and improving health worldwide. Our experienced scientists provide stand-alone bioanalytical services to the pharmaceutical, biopharmaceutical, animal health and medical device industries.

Kryptos Biotechnologies uses photothermal heating technology to generate and control heat for fast and accurate Polymerase Chain Reaction to build an integrated sample-to-answer molecular diagnostics system for point-of-care testing.  

Have you ever wondered what it would be like if a virus could talk? Well, wonder no more! Welcome to the latest episode of Join the Docs, where Professor Jonathan Sackier and Doctor Nigel Guest dive deep into the world of measles, discussing everything from its sneaky cause to its easy-to-spot symptoms and tricky diagnosis because there are no specific treatments, one needs sound prevention strategies.But wait, there's more! The Docs are joined by the one and only Ruby, who isn't just any guest—she's the personification of the measles virus itself! Ruby Ola spills the beans on ‘her' mischievous behaviour and the havoc she mischievously  visits on unsuspecting hosts.Ever thought about the history of measles and how it has evolved over time? The conversation takes a trip down memory lane, highlighting the ancient origins of this pesky virus and the rollercoaster ride of outbreaks and epidemics. Ruby emphasises how misinformation is her best friend and how it keeps her in the spotlight. She reminds everyone that evidence-based medicine is her arch-nemesis and the key to keeping her at bay.Why is vaccination so crucial in the fight against measles? The Docs stress the critical role of those tiny but mighty vaccines in preventing the resurgence of this highly contagious, very nasty and potentially fatal disease. Having explained how the whole “vaccine hesitancy” mythology evolved, they emphasise the importance of getting vaccinated. Ruby admits that she hates Mr. Vaccine because he ruins all her fun… what a prick!Throughout the episode, Professor Sackier and Dr. Guest wince atRuby's gleeful tales of her "adventures" inside the human body. They shake their heads in disbelief at her joy causing rashes and fevers, and how she boasts about evading the immune system's defences. Ruby's playful banter and over-the-top villain persona provide a creepy black comic relief, making the serious topic of measles education, yet strangely entertaining!. The episode wraps up with a strong message: don't let measles crash your party—get vaccinated and keep her out of your life for good! Give it a shot, it might just save your life.—--DISCLAIMER: The views and opinions expressed on Join the Docs are those of Dr. Nigel Guest, Jonathan Sackier and other people on our show. Be aware that Join the Docs is not intended to be medical advice, it is for information and entertainment purposes only - please, always take any health concerns to your doctor or other healthcare provider. We respect the privacy of patients and never identify individuals unless they have consented. We may change details, dates, place names and so on to protect privacy. Listening to Join the Docs, interacting on our social media, emailing or writing to us does not establish a doctor patient relationship.To Contact Us: For a deeper dive on this episode's issue, merchandise and exclusive content, head to www.jointhedocs.comFollow us on youtube.com/JoinTheDocs Follow us on instgram.com/JoinTheDocsFollow us on tiktok.com/JoinTheDocsFollow us on: facebok.com/JoinTheDocsFollow us on: x.com/JoinTheDocs

When you have a good thing going you often want it to last forever, but we know that can never happen. Life and the world around us are fluid, dynamic, and we're always finding the balance of fighting or harnessing entropy and inevitable change.As we encounter unexpected changes, we see them as chances to evaluate the foundations of our podcast's success while finding opportunities to evolve it and make it even better. Join us for a reflection of where we are, how we got here, and a sneak preview at what's to come. We're here to assure you, evolution is a good thing!

GET HEIRLOOM SEEDS & NON GMO SURVIVAL FOOD HERE: https://heavensharvest.com/ USE Code WAM to save 5%! GET ORGANIC COFFEE, MANUKA HONEY AND MUSHROOM TINCTURES HERE: https://madtravnutrition.com/ Use Code "Josh" and save 10%! Support the work of Jaymie Icke & Ickonic! HELP THE WAM LEGAL DEFENSE FUND HERE: https://gogetfunding.com/wam-legal-defense/ BUY GOLD HERE: https://firstnationalbullion.com/schedule-consult/ GET YOUR APRICOT SEEDS at the life-saving Richardson Nutritional Center HERE: https://rncstore.com/r?id=bg8qc1 Josh Sigurdson reports on the recent bird flu scare which has been propagandized as "100 times more deadly than covid" which in itself was a hoax. As it turns out, chickens are being rapid tested with PCR tests which are amplified to an extent that it will pick up on any illness a chicken once had. Polymerase Chain Reaction tests cannot test specifically for illnesses. It can only test for a spectrum of illnesses so the tests are false from the start. Essentially millions of chickens are being falsely tested positive and killed, much like humans with so-called covid in 2020 and 2021. For this reason, the USDA is calling for the decimation of the food supply to "save lives." Where have we heard that before? Meanwhile, Biden continues to target LNG imports and exports destroying the strength of the grid and therefore also damaging the food supply greatly. Farmers are showing the evidence of bugs ending up in the food supply without people noticing a change. mRNA is also being found in a lot of meat, though Tennesse is banning the use of mRNA lettuce. Another cargo boat has been hacked in New York City's harbor and was stopped just before crashing into a major bridge marking the 3rd example of this in only a week and a half. Another obvious attempt to utilize false flags to devastate the grid, economy and food supply in order to bring in technocratic totalitarian restrictions as a "solution" to move the world into the "Great Reset." Get prepared today! Stay tuned for more from WAM! ORDER QUALITY MEAT TO YOUR DOOR HERE: https://wildpastures.com/promos/save-20-for-life/bonus15?oid=6&affid=321 Save 20% and get $15 off your FIRST order! Support your local farms and stay healthy! HELP SUPPORT US AS WE DOCUMENT HISTORY HERE: https://gogetfunding.com/help-wam-cover-history/ GET AN EXTENDED FREE TRIAL FOR ICKONIC WHEN YOU SIGN UP HERE: https://www.ickonic.com/affiliate/josh10 BUY YOUR PRIVATE CLEARPHONE HERE: https://www.r1kln3trk.com/3PC4ZXC/F9D3HK/ LION ENERGY: Never Run Out Of Power! PREPARE NOW! https://www.r1kln3trk.com/3PC4ZXC/D2N14D/ GET VITAMINS AND SUPPLEMENTS FROM DR. ZELENKO HERE: https://zstacklife.com/?ref=WAM GET TIM'S FREE Portfolio Review HERE: https://bit.ly/redpilladvisor And become a client of Tim's at https://www.TheLibertyAdvisor.com STOCK UP ON STOREABLE FOODS HERE: http://wamsurvival.com/ OUR GOGETFUNDING CAMPAIGN: https://gogetfunding.com/help-keep-wam-alive/ OUR PODBEAN CHANNEL: https://worldaltmedia.podbean.com/ Find us on Vigilante TV HERE: https://vigilante.tv/c/world_alternative_media/videos?s=1 FIND US on Rokfin HERE: https://rokfin.com/worldalternativemedia FIND US on Gettr HERE: https://www.gettr.com/user/worldaltmedia See our EPICFUNDME HERE: https://epicfundme.com/251-world-alternative-media JOIN OUR NEWSLETTER HERE: https://www.iambanned.com/ JOIN our Telegram Group HERE: https://t.me/worldalternativemedia JOIN US on Rumble Here: https://rumble.com/c/c-312314 FIND WAM MERCHANDISE HERE: https://teespring.com/stores/world-alternative-media FIND OUR CoinTree page here: https://cointr.ee/joshsigurdson JOIN US on SubscribeStar here: https://www.subscribestar.com/world-alternative-media We will soon be doing subscriber only content! Follow us on Twitter here: https://twitter.com/WorldAltMedia Help keep independent media alive! Pledge here! Just a dollar a month can help us alive! https://www.patreon.com/user?u=2652072&ty=h&u=2652072 BITCOIN ADDRESS: 18d1WEnYYhBRgZVbeyLr6UfiJhrQygcgNU World Alternative Media 2024

In this episode, Colin C. Pritchard, MD, PhD, a pathologist, and Heather H. Cheng, MD, PhD, a medical oncologist, discuss optimal biomarker testing to guide treatment decisions in advanced prostate cancer, with topics including: Rationale for targeting PARP in prostate cancer with ARI combinationsStudy design nuances and findings from key randomized phase III clinical trials evaluating combination therapy with a PARP inhibitor and ARI, including PROpel, MAGNITUDE, and TALAPRO-2FDA approvals of combination therapy with a PARP inhibitor and ARI, including a comparison of populations based on mutations Optimal biomarker testing for gene mutations in homologous recombination and mismatch repair pathwaysTips for optimal coordination between pathology and medical oncologyPresenters: Heather H. Cheng, MD, PhDAssociate ProfessorDepartment of MedicineDivision of Hematology and OncologyAttending PhysicianDepartment of Genitourinary Medical OncologyFred Hutchinson Cancer CenterSeattle, WashingtonColin C. Pritchard, MD, PhDCo-DirectorGenetics and Solid Tumors LaboratoryUniversity of Washington Medical CenterProfessorDepartment of Laboratory Medicine and PathologyUniversity of WashingtonSeattle, WashingtonContent based on an online CME program supported by an independent educational grant from Pfizer, Inc.Link to full program: https://bit.ly/3PFagxb

Welcome back Gene-iuses!  Jordan and Cassie kick us off with a fun teaser of what's to come in Season 2 of the Absolute Gene-ius series.   We'll be featuring another great season of interesting guests, all using dPCR to progress their diverse research applications. This includes conversations about reproductive biology, liquid biopsy and transplantation research, CAR-T research, the role of bioinformatics in PCR assay design, micro-RNA analysis, differential gene expression analysis, and of course Cassie's Career Corner, where we get to learn about people behind the science.   This teaser, like every episode of Absolute Gene-ius, has the fun baked in to keep it all light and interesting too.  You might even hear some digital PCR jokes!  Visit the Absolute Gene-ius page to access the entire first season and to learn more about the hosts and the Applied Biosystems QuantStudio Absolute Q Digital PCR System.  

David Liew speaks with infectious diseases physician Ella Meumann. They discuss the latest developments in COVID-19 testing and how the pandemic affected our virus-testing strategies. Read the full article by Ella and her co-author, Jennifer Robson, in Australian Prescriber. This interview was conducted on 28 August 2023.

As scientists hone the ability to extract meaningful information from DNA found in the air, Shambhavi Naik and Saurabh Todi discuss the policy challenges this new technological progress presents. Do follow IVM Podcasts on social media. We are @‌IVMPodcasts on Facebook, Twitter, & Instagram. https://twitter.com/IVMPodcasts https://www.instagram.com/ivmpodcasts/?hl=en https://www.facebook.com/ivmpodcasts/ You can check out our website at https://shows.ivmpodcasts.com/featured Follow the show across platforms: Spotify, Google Podcasts, Apple Podcasts, JioSaavn, Gaana, Amazon Music Do share the word with your folks!See omnystudio.com/listener for privacy information.

In this episode, Renslow Sherer, MD, and Trinh P. Vu, PharmD, BCIDP, discuss strategies for managing ambulatory patients with acute or previous COVID-19 infection, including:Current COVID-19 landscapeRapid antigen tests and PCR testsRisk stratification of patients who have a positive SARS-CoV-2 testAntiviral treatment (nirmatrelvir + ritonavir, remdesivir, and molnupiravir)Long COVIDPresenters:Renslow Sherer, MDDirector, International HIV Training CenterProfessor of MedicineSection of Infectious Diseases and Global HealthDepartment of MedicineUniversity of ChicagoChicago, IllinoisTrinh P. Vu, PharmD, BCIDPClinical Pharmacy Specialist in Infectious DiseasesDepartment of Pharmaceutical ServicesEmory University Hospital MidtownAtlanta, GeorgiaTo download the slides: bit.ly/3oHKC09To view the full online program: bit.ly/4201xcO

In this episode, Renslow Sherer, MD, and Trinh P. Vu, PharmD, BCIDP, discuss strategies for managing ambulatory patients with acute or previous COVID-19 infection, including:Current COVID-19 landscapeRapid antigen tests and PCR testsRisk stratification of patients who have a positive SARS-CoV-2 testAntiviral treatment (nirmatrelvir + ritonavir, remdesivir, and molnupiravir)Long COVIDPresenters:Renslow Sherer, MDDirector, International HIV Training CenterProfessor of MedicineSection of Infectious Diseases and Global HealthDepartment of MedicineUniversity of ChicagoChicago, IllinoisTrinh P. Vu, PharmD, BCIDPClinical Pharmacy Specialist in Infectious DiseasesDepartment of Pharmaceutical ServicesEmory University Hospital MidtownAtlanta, GeorgiaTo download the slides: bit.ly/3oHKC09To view the full online program: bit.ly/4201xcO

In this episode, Renslow Sherer, MD, and Trinh P. Vu, PharmD, BCIDP, discuss strategies for managing ambulatory patients with acute or previous COVID-19 infection, including:Current COVID-19 landscapeRapid antigen tests and PCR testsRisk stratification of patients who have a positive SARS-CoV-2 testAntiviral treatment (nirmatrelvir + ritonavir, remdesivir, and molnupiravir)Long COVIDPresenters:Renslow Sherer, MDDirector, International HIV Training CenterProfessor of MedicineSection of Infectious Diseases and Global HealthDepartment of MedicineUniversity of ChicagoChicago, IllinoisTrinh P. Vu, PharmD, BCIDPClinical Pharmacy Specialist in Infectious DiseasesDepartment of Pharmaceutical ServicesEmory University Hospital MidtownAtlanta, GeorgiaTo download the slides: bit.ly/3oHKC09To view the full online program: bit.ly/4201xcO

In this episode, Tracey Piparo, PA-C, and Renslow Sherer, MD, discuss cases of nonhospitalized patients with COVID-19, including:Young, healthy patient with no risk factorsYoung, healthy patient with risk factorsOlder patient with immunocompromiseOlder patient with renal dysfunctionPatient experiencing hypoxia Presenters: Tracey Piparo, PA-C Department of Palliative Medicine RJWBarnabas New Brunswick, New Jersey Renslow Sherer, MD Director, International HIV Training Center Professor of Medicine Section of Infectious Diseases and Global Health Department of Medicine University of Chicago Chicago, Illinois To download the slides: bit.ly/44cgH0qTo view the full online program: https://bit.ly/4201xcO

In this episode, Renslow Sherer, MD, and Jason E. Farley, PhD, MPH, ANP-BC, FAAN, FAANP, AACRN, discuss patient cases that illustrate key concepts for managing ambulatory patients with acute COVID-19 infection, including:A young, otherwise healthy patientAn older patient with multiple comorbidities A patient with immunocompromiseA patient requiring emergency department-level carePresenters:Renslow Sherer, MDDirector, International HIV Training CenterProfessor of MedicineSection of Infectious Diseases and Global HealthDepartment of MedicineUniversity of ChicagoChicago, IllinoisJason E. Farley, PhD, MPH, ANP-BC, FAAN, FAANP, AACRNProfessorSchool of NursingJohns Hopkins UniversityNurse PractitionerDepartment of Infectious DiseasesJohn G. Bartlett Specialty PracticeBaltimore, MarylandTo download the slides:http://bit.ly/3G9IrJxTo view the full online program:https://bit.ly/3TqnNIX

In this episode, Renslow Sherer, MD, and Trinh P. Vu, PharmD, BCIDP, discuss strategies for managing ambulatory patients with acute or previous COVID-19 infection, including:Rapid antigen tests and PCR testsRisk stratification of patients who have a positive SARS-CoV-2 testAntiviral treatment (nirmatrelvir + ritonavir, remdesivir, and molnupiravir)Monoclonal antibody treatment (bebtelovimab)Long COVIDPresenters:Renslow Sherer, MDDirector, International HIV Training CenterProfessor of MedicineSection of Infectious Diseases and Global HealthDepartment of MedicineUniversity of ChicagoChicago, IllinoisTrinh P. Vu, PharmD, BCIDPClinical Pharmacy Specialist in Infectious DiseasesDepartment of Pharmaceutical ServicesEmory University Hospital MidtownAtlanta, GeorgiaTo download the slides:https://bit.ly/3TkT7sJTo view the full online program:https://bit.ly/3TqnNIX

I hope you are not prone to regrets.  The next time you make the wrong decision, I want you to look back and remember that it seemed like a good idea at the time. You were given incomplete information. The future was unknowable. What is there to regret? Nevertheless, the dull ache of regret came upon me when Kary Mullis died without warning.  I loved Kary for his sense of humor and his wit, and I will always cherish what he wrote in my copy of his book, Dancing Naked in the Mind Field.  And then Loren Lewis died without giving me a heads-up.  Loren was never a father-figure; he was my outrageous older brother. He was bombastic and vain and he taught me how to get things done when I didn't have any money, and he would have taken a bullet for me. And then Perry McKee walked over the horizon without a wink or a wave good-bye.  Perry was extraverted and impulsive and he made everyone laugh. When we were 14, Perry decided the day had finally arrived that he should light a fart and become the world's first jet-propelled human. He wanted me to hold the match for him but I vigorously declined, so Ernie Henry held the match as the rest of us stood anxiously outside the closed door of Perry's windowless bathroom. It was Brother McKee's deep conviction that the miracle of jet-propulsion should be observed in total darkness. When Perry bellowed like a bull and tumbled out the doorway, we knew that Ernie had held the match too close. Ernie Henry is gone now, too.  The immortals from my past are disappearing.The last time I spoke to Kary Mullis, Loren Lewis, Perry McKee and Ernie Henry, I didn't know that it would be the last time I spoke to them. My only regrets are the things I left unsaid. Please don't read too much into these musings. I'm fine. Pennie is fine. No one is dying. It's just that time of year.  The green of the grass is soaking back into the earth and the leaves are turning red and orange. Children are gathering into rooms again where an adult tells them not to talk. Men are chasing a tapered leather ball as escaped convicts blow whistles and toss their handkerchiefs into the air. I look for Andy Griffith to ask if he wants to get a Big Orange drink, but Andy is nowhere to be found.  It won't be long before my lawn pulls a white blanket up to its chin, just outside my front door. The squirrel in his cap and the plants in burlap will all settle down for a long winter's nap.  And then Springtime will pierce the pale heart of winter with a shout of green and a blade of grass, and we will dress in bright colors for Easter. Kary Mullis opened the door of genetic research when he invented Polymerase Chain Reaction. Loren Lewis opened the future of a 15-year-old boy when he showed him how to be unafraid. Perry McKee and Ernie Henry had no regrets. It seemed like a good idea at the time. Roy H. Williams Steve Curtin is ranked as one of the top 30 customer service experts in the world. His clients include Carnival Cruise Line, NAPA Auto Parts, and TJ Maxx. Steve believes every owner and every manager needs to have “the conversation” with every employee about why their job matters, and why their company matters. “The NASA janitor wasn't mopping floors; he was helping to send a man to the moon.” Imagine what would happen if your employees felt the same way about the greater purpose of the work they do in your company! Steve Curtin and roving reporter Rotbart talk about it at MondayMorningRadio.com.

How well do you know Dubai? To many people, it's a country that you only layover in. But if you ever decide to stay and live there, chances are traveling or working there brings its own sets of challenges. This episode aims to show what it's like living or traveling in Dubai; and what rules you should follow to avoid getting into trouble. Episode highlights:Documents you need before entering Dubai.Things and attitude that will get you in trouble.Best time and season to visit.Activities and best places to visit in Dubai.How to keep yourself safe while traveling within Dubai. Quote shared in this episode:"Take only memories. Leave only footprints." ― Chief Seattle Youtube channel mentioned in this episode: Adoptee Kwento Kwento Have questions, comments, or concerns? We'd love to hear from you. Email us at hello@taraletstalk.com.  Subscribe:  Apple Podcasts | Google Podcasts | Spotify | Stitcher | Audible | Amazon Music | Goodpods | iHeartRADIO |  If you enjoyed this episode, please don't forget to give us a five star rating. Or leave us a review on iTunes, Podcast Addict, Podchaser and Castbox. Follow us on Social Media:Taralets Talk Podcast on IGTaralets Talk Podcast on Facebook Taralets Talk is sponsored by Disenyo.co LLC:DISCLAIMER: The opinions, beliefs, and viewpoints expressed by the hosts and guests on this podcast do not necessarily represent or reflect the official policy, opinions, beliefs, and viewpoints of Disenyo.co LLC and its employees. 

In this episode, we review the high-yield topic of Polymerase Chain Reaction (PCR) from the Biochemistry section. Follow Medbullets on social media: Facebook: www.facebook.com/medbullets Instagram: www.instagram.com/medbulletsofficial Twitter: www.twitter.com/medbulletsIn this episode --- Send in a voice message: https://anchor.fm/medbulletsstep1/message

Kevin McKernan is a renowned leader in DNA sequencing technology. He led a research team part of the original Human Genome Project and is the founder and Chief Science Officer of Medicinal Genomics, a life sciences company that distributes genetics-based platforms for quality and safety testing of legal cannabis.Nick and Kevin discussed the Cannabis genome, the genome of Psilocybe cubensis (magic mushrooms); Polymerase Chain Reaction (PCR) technology, how it works, and how it's used for COVID-19 testing; cannabinoids such as cannabidiol (CBD) and recent research indicating their potential anti-SARS-CoV-2 effects; blockchain technology for genomics data; and mRNA vaccines and SARS-CoV-2.USEFUL LINKSSign up for the weekly Mind & Matter newsletter[https://mindandmatter.substack.com/?sort=top]Download the podcast & follow Nick at his website[https://www.nickjikomes.com]Follow Nick's work through Linktree:[https://linktr.ee/trikomes]Athletic Greens, comprehensive daily nutrition (Free 1-year supply Vitamin D w/ purchase)[https://www.athleticgreens.com/mindandmatter]Organize your digital highlights & notes w/ Readwise (2 months free w/ sub)[https://readwise.io/nickjikomes/]Learn more about our podcast sponsor, Dosist[https://dosist.com]Support the show (https://www.patreon.com/nickjikomes)

The Federal Government has imposed travel restrictions on passengers who shun the COVID-19 Polymerase Chain Reaction test.Chairman of the Presidential Steering Committee on COVID-19 and Secretary to the Government of the Federation, Boss Mustapha says that the restrictions came into effect on September 14.He explained that passengers arriving in Nigeria must go through the routine port health screening and present electronic or print-out evidence of pre-boarding COVID-19 PCR test and the Permit to Travel Certificate/QR Code.The SGF says passengers who fail to show up for this test will face travel restrictions on their passports for at least six months and will not be able to travel abroad for this period.

The Federal Government has imposed travel restrictions on passengers who shun the COVID-19 Polymerase Chain Reaction test.Chairman of the Presidential Steering Committee on COVID-19 and Secretary to the Government of the Federation, Boss Mustapha says that the restrictions came into effect on September 14.He explained that passengers arriving in Nigeria must go through the routine port health screening and present electronic or print-out evidence of pre-boarding COVID-19 PCR test and the Permit to Travel Certificate/QR Code.The SGF says passengers who fail to show up for this test will face travel restrictions on their passports for at least six months and will not be able to travel abroad for this period.

The Federal Government has imposed travel restrictions on passengers who shun the COVID-19 Polymerase Chain Reaction test.Chairman of the Presidential Steering Committee on COVID-19 and Secretary to the Government of the Federation, Boss Mustapha says that the restrictions came into effect on September 14.He explained that passengers arriving in Nigeria must go through the routine port health screening and present electronic or print-out evidence of pre-boarding COVID-19 PCR test and the Permit to Travel Certificate/QR Code.The SGF says passengers who fail to show up for this test will face travel restrictions on their passports for at least six months and will not be able to travel abroad for this period.

Learn how COVID tests work; how math can help you with major life decisions; and how some dogs have natural talent. How COVID PCR tests work by Ashley Hamer (Listener question from Lydia) Tiner, S. (2020, March 27). The Science Behind the Test for the COVID-19 VirusDiscovery's Edge. Https://Discoverysedge.mayo.edu/. https://discoverysedge.mayo.edu/2020/03/27/the-science-behind-the-test-for-the-covid-19-virus/ ‌ Amoeba Sisters. (2020). PCR (Polymerase Chain Reaction) [YouTube Video]. In YouTube. https://www.youtube.com/watch?v=a5jmdh9AnS4  ‌PCR: Thirty-five years and counting. (2018, May 10). PCR: Thirty-five years and counting. Science | AAAS. https://www.sciencemag.org/features/2018/05/pcr-thirty-five-years-and-counting ‌ Ask A Scientist Staff. (2020, October 23). Why qPCR is the gold standard for COVID-19 testing. Ask a Scientist; Thermo Fisher Scientific. https://www.thermofisher.com/blog/ask-a-scientist/why-qpcr-is-the-gold-standard-for-covid-19-testing/  Additional resources from David Sumpter: Pick up "The Ten Equations that Rule the World and How You Can Use Them Too": https://www.penguin.co.uk/books/314/314406/the-ten-equations-that-rule-the-world/9780241404546.html  Faculty page: https://katalog.uu.se/profile/?id=N7-525  Twitter: https://twitter.com/soccermatics?lang=en  Dogs have talent, yes they do by Steffie Drucker Not only humans got talent, dogs got it too! (2021, July 7). EurekAlert! https://www.eurekalert.org/news-releases/553737  Fugazza, C., Dror, S., Sommese, A., Temesi, A., & Miklósi, Á. (2021). Word learning dogs (Canis familiaris) provide an animal model for studying exceptional performance. Scientific Reports, 11(1). https://doi.org/10.1038/s41598-021-93581-2  ‌Genius Dog Challenge. (2021). Exceptional DOGS & what they can TEACH us [YouTube Video]. In YouTube. https://www.youtube.com/watch?v=WF6ZpjdH2Sc  Follow Christina Hunger, Speech Pathologist, on Instagram @Hunger4Words https://www.instagram.com/hunger4words/?hl=en  Follow Curiosity Daily on your favorite podcast app to learn something new every day withCody Gough andAshley Hamer. Still curious? Get exclusive science shows, nature documentaries, and more real-life entertainment on discovery+! Go to https://discoveryplus.com/curiosity to start your 7-day free trial. discovery+ is currently only available for US subscribers. See omnystudio.com/listener for privacy information.

In part one of our fifth episode, RCPA President Dr Michael Dray, discusses COVID-19 PCR lab testing with RCPA Past President, Dr Michael Harrison. Dr Michael Harrison is CEO of Sullivan Nicolaides Pathology, Brisbane. SNP is one of Australia's largest diagnostic pathology laboratories and undertakes large scale PCR (polymerase chain reaction) testing daily.Copyright © 2021 The Royal College of Pathologists of Australasia. All rights reserved.

Guest: Susan Butler-Wu, PhD, D(ABMM), SM(ASCP) Dr. Susan Butler-Wu, Associate Professor of Clinical Pathology and Director of Medical Microbiology at LAC+USC Medical Center, investigates the differences—and highlights the benefits—of PCR versus antigen testing for COVID-19.

(00:31):But before we get started, Dr. Pritt, could you provide our listeners with a little bit about you and your background? (01:22):Can you provide a brief overview and intended use of this testing? (03:16):Could you describe what clinical action is enabled by the results of this test? (05:31):Now let's discuss which patients should have this testing and when should it be performed?' (06:56):Can you now explain, how does this test improve upon previous testing approaches? (08:04):Are there other options on the market? If so, what makes our testing different or unique?

Joseph Yao, M.D., chair of Mayo Clinic’s Division of Clinical Microbiology, joins this episode of the "Answers From the Lab" podcast. Dr. Yao and Bobbi Pritt, M.D., discuss the current status of COVID-19 testing, including tests that can be used to detect variants of the virus.

Anja Roden, M.D., a medical director for Mayo Clinic's Immunohistochemistry Laboratory, joins this episode of the "Answers From the Lab" podcast. Dr. Roden and Bobbi Pritt, M.D., discuss the development and evaluation of immunohistochemistry in situ hybridization and polymerase chain reaction for detection of SARS-CoV-2 in formalin-fixed paraffin-embedded tissues.

"Global Synchronizer" Hosts: Darren Weeks, Vicky Davis Show website: https://governamerica.com Vicky's Websites: https://thetechnocratictyranny.com http://channelingreality.com COMPLETE SHOW NOTES AND CREDITS AT: https://governamerica.com/radio/radio-archives/22386-govern-america-november-28-2020-global-synchronizer Listen LIVE every Saturday at 11AM Eastern time at http://live.governamerica.com Text GOVERN to 80123 to be notified of special shows and emergency transmissions outside of our regularly-scheduled Saturday broadcast. Transmissions are moved when circumstances warrant. Justice Department asserts its unreviewable discretion to kill American citizens. Community Policing continues to ramp in Washington state, as "community groups" are brought in to decide punishments of some felons. Pope Francis supports government tyranny. Trump legal team distances itself from Sydney Powell. Iran nuclear scientist assassinated as President Trump orders 2,500 troops out of Afghanistan. Anthony Fauci's wife is chief bioethicist for human subjects. Conflict of Interest? State officials continue to push the boundaries of tyranny with COVID restrictions. Will vaccine resisters be forced out of work? Digital travel credentials, biometric smart cards, civic digital identity apps, contact tracing, "freedom passes", snitches and more. Deep dive into the Polymerase Chain Reaction (PCR) from Kary Mullis, the man who won the Nobel prize for inventing it, and more. Phone calls.

Anyone who has worked with DNA in the laboratory is undoubtedly familiar with the polymerase chain reaction - PCR, as it’s usually known.Invented in 1985, PCR is an indispensable molecular biology tool that can replicate any stretch of DNA, copying it billions of times in a matter of hours, providing enough DNA to use in sequencing or further research, or for applications like forensics, genetic testing, ancient DNA analysis or medical diagnostics.It’s hard to overstate the transformation that PCR brought to the world of molecular biology and biomedical research. Suddenly, researchers could amplify and study DNA in a way that had been simply impossible before, kickstarting the genetic revolution that’s still going strong today.So where did this revolutionary technology come from? Officially, PCR was invented in 1985 by a colourful character called Kary Mullis, who won a Nobel Prize for the discovery (more on him later). But, as we’ll see, all the components of PCR were in place by the early 1980s - it just took a creative leap to assemble them into one blockbusting technique.Listen to the full episode and find a transcript at GeneticsUnzipped.com.Genetics Unzipped is the podcast from the UK Genetics Society and is produced by First Create the Media. Follow Genetics Unzipped @geneticsunzip, and the Genetics Society @GenSocUK on Twitter.Listen to Genetics Unzipped on Apple podcasts (iTunes), Spotify, or wherever you get your podcasts.

William Morice, II, M.D., Ph.D., chair of the Department of Laboratory Medicine and Pathology at Mayo Clinic and president of Mayo Clinic Laboratories, joins the "Answers From the Lab" podcast for his weekly leadership update. In this episode, Dr. Morice and Bobbi Pritt, M.D., review the testing options currently available to detect COVID-19.

While virologists and risk analysts have been theorising about the effects of a global pandemic for years, few could have imagined the Covid-19 pandemic’s transformative impact on society. Even after the World Health Organisation declared Covid-19 a public health emergency in January, “how many people, globally, would’ve thought that the Covid-19 would have such a profound impact on the global economy, and the global population?” posits Minerals Council South Africa CEO Roger Baxter. He notes that, fortunately, the local mining industry’s collaboration on other health crises, such as the HIV/Aids epidemic and the country’s persistent challenges with tuberculosis, meant that it was perhaps one of the few industries able to adequately respond and mitigate the spread of the disease. This is evidenced by the fact that the local industry has a mortality rate of 0.1%, or roughly half that of the country, as well as a test rate of 11.83%, which outstrips South Africa’s (7.41%) and the world’s (9.04%) test rate. Aside from its prior experience with health crises, the effectiveness of the industry’s response is owed to its willingness to collaborate and to its proactivity. As Minerals Council spokesperson Charmane Russell explains: “We really got out of the starting blocks early, in terms of communicating with members and other entities. In fact, before the Investing in African Mining Indaba in February, the industry had recognised the virus as a potential threat. That’s when we started producing material for member companies to educate employees on what Covid-19 was and how to prevent transmission.” Baxter notes that the Minerals Council’s guideline publications, including the 10-point plan and the standard operating procedures (SOPs), were instrumental in establishing control measures based on risk, prevention and mitigation, and that significant portions of the SOPs were reflected in the regulations published by the Department of Mineral Resources and Energy (DMRE). He adds that, during the height of the pandemic, the council convened fortnightly CEO Zero Harm forums, during which company CEOs would discuss their measures for addressing the Covid-19 pandemic, to share lessons and successes. Efforts in Testing “In February, well before the first case was diagnosed in South Africa, we recognised that the country would not have sufficient resources to adequately address the pandemic. Strong, advanced and well-resourced health systems in developed countries had struggled and so the initial focus was on increasing capacity and acquiring resources,” explains Minerals Council public affairs and transformation senior executive Tebello Chabana. He adds: “When it was clear that this public health emergency was becoming a pandemic, it became evident that there was a global shortage of testing equipment. Further, it was clear that South Africa’s public testing capacity would become constrained, as many countries were restricting the export of equipment and consumables, and back then there were fewer manufacturers.” He explains that, some of the member companies were “quite visionary”, looking into means of acquiring Polymerase Chain Reaction machines and other equipment such as storage fridges, biosafety cabinets and centrifuges to increase testing capacity and analyse test samples to help speed up diagnoses and curb the spread of the virus. “Companies, individually and through the Minerals Council, agreed to shore up the country’s testing capacity, because at the end of the day, while we needed to test employees, there was also a moral obligation to assist in testing communities. “This pandemic was novel, it was unprecedented, and so companies couldn’t put their hands up and say that government must do everything. Bearing in mind that it is not an occupational disease. Employees were always more likely to contract it outside the mine. Therefore our intervention had to include supporting the government by testing the publ...

Today our expert Infectious Disease and Community Medicine doctors discuss the latest on COVID-19. We discuss how the pandemic is affecting immigrants seeking asylum, how the Rochester Institute of Technology is testing sewage for coronavirus, and how Polymerase Chain Reaction analysis can indicate the severity of the virus. As always, join us for all the COVID-19 information you need, explained in clear terms by health experts. --- Send in a voice message: https://anchor.fm/noisefilter/message

Denaturation (Heat to high temp), Primer Annealing (Cool slightly), Primer Extension via polymerase (Heat but not as much as step 1).

Katie and Cory talk about the 80s computing power, the definition of cheating, conservation of energy and LSD. Review our podcast! Head to RateThisPodcast.com/teachme and give us a review. Hit us up on Twitter @TeachMeShow or send us love letters via email: podcast@teachme.show TMSG is a proud member of the Apocalypse Podcast Network. Find your next favorite […]

COVID-19 Testing-Polymerase Chain Reaction (PCR) | Dr RR Baliga's 'Got Knowledge Doc' Podcasts for Physicians Not Medical Advice or Opinion

Yes, Numbers Matter Richard Gale and Gary Null PhD Progressive Radio Network, April 7, 2020   For the millions of people who are following the daily reports and analyses of the COVID-19 pandemic, one thing should be evident. There are far more questions arising than answers, and there are certainly less answers to be relied upon. Consequently, we are being inundated with numbers and more numbers. The number of cases of infection. The number of tests performed or that should be performed. The number of masks, hospital beds and ventilators necessary. And the number of fatalities. Consider the early predictions made by NIAID director Anthony Fauci that we might expect 1.7 million deaths only to later reduce his numbers to that of a regular flu season. Or there is one of the nation's chief physicians serving Congress and the Supreme Court, Dr. Brian Monahan, who pulled unsubstantiated figures from the astral plane to warn NBC News that up to 150 million Americans will become infected.  Moreover, confusion seems to be rampant throughout much of the nation's healthcare infrastructure. We are being deprived of basic facts because even the experts don't have them. Perhaps we have all seen the images of medical personnel having to wear black garbage bags as protective gear in a New York hospital or nurses' reports about having to wash and share face masks due to shortages. One revelation that COVID-19 is mirroring back to us is dire state of our corporate healthcare system and the dearth of doctors compared to other developed nations. But should we be surprised about the failures of American medicine? Funding that should go towards a resilient medical infrastructure and preventative health measures in times of emergency has been neglected for decades. If the nation's healthcare infrastructure had progressed to meet its population's demand it would have lessened the pandemic significantly. Instead the US budget has been funneled off to sustain our imperialist military adventures and provide bailouts for the mega-transnational corporations, banks and billionaires.  Part of the problem is that there are no accurate epidemiological and statistical analyses, even for fatality numbers, that are based upon sound scientific principles. Perhaps one of the clearest examples to try to understand this confusion is to look at the huge discrepancy in infection rates and deaths between different countries, especially neighboring countries in Europe. How does one account for an approximate 1,810 deaths per 103,300 cases in Germany with 8,900 deaths per 98,000 cases right next door in France? Each has comparable economies and advanced healthcare systems. Is it that there are no reliable mechanisms for proper data collection and analysis? It seems to be that every government is doing its own thing and therefore all the data we have to determine the severity of the pandemic is skewed. The distinguished physician and German politician Dr. Wolfgang Wodarg, who earlier declared the H1N1 swine flu pandemic as "one of the greatest medicine scandals of the century," has again warned that the COVID-19 hype is causing "considerable damage to our freedom and personal rights through frivolous and unjustified quarantine measures and restrictions." Commenting on the current situation, Dr. Wodarg argues that "evidence based epidemiological assessment is drowning in the mainstream of fear mongers in labs, media and ministries." In the US, which closely follows (or controls) the World Health Organization (WHO), it is a larger mess. For example, WHO's recent guidelines divide COVID-19 cases into two categories. First are those cases that have been confirmed by properly identifying the virus through laboratory testing. The second are those cases that have been "assigned to a clinical or epidemiology diagnosis of COVID-19 where laboratory confirmation is inconclusive or not available." In other words, people are being ruled as COVID-19 positive without any laboratory confirmation. Furthermore, the WHO now recommends that deaths in this second category should be contributed to the coronavirus.  One does not require a medical degree to realize how this is resulting in faulty tabulation of infection and death rates.  The US has also adopted the WHO guidelines according to the National Vital Statistics System. A NVSS document explaining how deaths should be reported on death certificates states, "COVID-19 should be reported on the death certificate for all decedents where the disease caused or is assumed to have caused or contributed to death." So what impact would this have on fatality numbers if deaths without laboratory confirmation are in fact caused by other illnesses? We already know that the virus is most lethal to the elderly and persons with pre-existing medical conditions.  Below is a chart produced by the NVSS showing weekly pneumonia deaths from 2013 to the present. Note the dramatic unprecedented decrease in deaths associated with pneumonia since the arrival of COVID-19 in the US. How does that happen? Is there an invisible war being waged between COVID-19 and pneumonia-causing pathogens such as flu, respiratory syncytial virus (RSV), bacteria, mycoplasma and fungi to infect victims? The chart is nothing more than an illusion. The reason for this remarkable decrease is simply human error and scientific negligence.  Likewise, as both confirmed and assumed cases of COVID-19 climb, influenza cases are falling. To add to the confusion, just as the CDC now tallies COVID-19 deaths, it has done the same by conflating influenza and pneumonia deaths for many years. The CDC claims approximately 34,000 people die from flu infections annually. However, no distinction is made between the two and influenza and pneumonia deaths are grouped together. It is an excellent propaganda tool for keeping people nervous when flu season arrives in order to get rid of and distribute the 160 million-plus vaccine doses our tax dollars purchase every year.  As physician Dr. Ken Stoller wrote to the Office of the Assistant Secretary for Planning and Evaluation at the Department of Health and Human Services,  "US data on influenza deaths are false and misleading. The Centers for Disease Control and Prevention acknowledges a difference between flu death and flu associated death yet uses the terms interchangeably. Additionally, there are significant statistical incompatibilities between official estimates and national vital statistics data. Compounding these problems is a marketing of fear—a CDC communications strategy in which medical experts "predict dire outcomes" during flu seasons." And now we discover the same agency re-categorizing influenza and pneumonia cases as COVID-19. In the same vein, how many deaths assigned to the flu may actually be caused by a coronavirus?  Dr. Wodarg notes that it is "the well known fact that in every 'flu wave' 7-15% of acute respiratory illnesses (ARI) are coming along with coronaviruses." He further warns that "we are currently not measuring the incidence of coronavirus diseases but the activity of the specialists searching for them." We still do not know COVID-19's pathogenetic role and impact compared to the common seasonal flu.  Even if we erroneously assume the CDC's figures are accurate, as of today, there are 364,700 cases of coronavirus and almost 11,000 deaths. Yet this is a far cry from annual illnesses and deaths associated with the flu. Last year, according to Johns Hopkins University, there were over 35 million cases of flu infection and over 34,100 deaths (see chart below). And when did the entire economy ever shut down during a flu season?  Even during the 2009-2010 H1N1 swine flu scare -- the hyped-up pandemic that never was and was estimated to have killed only 17,000 people -- life went on as normal. The WHO's faux H1N1 pandemic resulted in only half of the CDC's conventional estimates for annual deaths. The world's experts at the WHO, British Health Ministry, and the CDC could not have been more wrong in their predictions in their fear tactics.  Curiously, the Johns Hopkins researchers estimated 61,000 deaths during the 2017-2018 flu season while the CDC reported it being over 80,000, a 25% difference. This also completely ignores co-morbidity, deaths directly due to other medical complications, usually pre-existing, that are independent from the virus.  Equally challenging is the lack of a universal and accurate test for proper viral identification. Polymerase Chain Reaction testing or PCR is the most accurate but is not being utilized at the level it should due to the lack of PCR systems and facilities for high throughput testing. People go to a hospital emergency room and are often only checked and asked about their symptoms. Rapid influenza tests may be performed and if negative a patient is diagnosed with a positive coronavirus diagnosis. This is not sound medical protocol. It is divination.  For whatever reason, America has an allergic reaction against learning anything from previous mistakes in the face of emergencies, whether it is a real crisis such as hurricanes Katrina and Sandy, or false alerts such as the 2009 swine flu scare. We may also remember that the aggressive push to fast track an inadequately studied swine flu vaccine onto the market resulted in spontaneous abortions and miscarriages by pregnant women who were persuaded to receive the vaccine.  But there are some facts we know. Physical distancing, wearing masks, and conscientiously washing hands and cleaning surfaces work as several Asian countries have proven. Perhaps this is a cultural thing. East Asians tend to have a greater sense of civil responsibility towards their neighbors than Americans who regard wearing a mask in public or entering self-isolation as an annoyance and inconvenience. For years, Chinese, Japanese and Korean citizens have frequently worn masks if they are feeling ill in order to protect their those they come in contact with. Other nations have been able to contain the spread of COVID-19; unfortunately Americans' superiority complex of global exceptionalism has been one of our worst enemies. In the meantime we desperately need scientifically valid numbers. No more politicized numbers that unnecessarily stress people out, further weaken their immune systems, and spiral the nation into further confusion and panic. 

Matt Landman is an activist and the creator of the documentary Frankenskies. In this super-show we sift through the scientific statistics and ask several important questions that throw the mainstream explanation of the COVID-19 crisis into serious doubt. We cover a lot of ground on this one with the hope of keeping you grounded during the storm. Topics include the unproven theory of the what the virus even is, the enormous flaws in PCR testing (Polymerase Chain Reaction), the facts that impact the death statistics, and the problematic treatment protocols, and that's just scratching the surface. We also look at the global lockdown quarantine, how its effects on the economy could lead to drastic totalitarian action, the evidence of a "plan-demic" plot to capitalize on crisis, corporate CEOs and government insiders cashing out their stocks, and the unlikelihood that 5G is to blame (although its still not a good thing). To top things off we examine some of the super-sketchy anti-freedom laws that are being considered in Congress. All that and much more, so tune in and enjoy a fresh perspective on these strange times.SUPPORT INNERVERSEBecome a Plus+ Member for extended episodes & early access to shows!https://www.patreon.com/innerverseRESOURCESDavid Crowe's "Coronavirus Panic" White Paper With Official Data Citations & Bibliography - LINKPODCASTSWhat Really Makes Us Ill, Germ Theory, & The Four Factors - LINK Coronavirus Chaos, Mr. Global, & The Technocratic Plot - LINK Coronavirus COVID-19: The Risks, The Testing, & The Treatments - LINK FIND MATT LANDMAN ONLINEFrankenskies film - https://www.frankenskiesthemovie.comSPERO Protection Clothing - https://speroprotectionclothing.com/Actual Activists - https://www.actualactivists.comMatt Landman on Facebook @Frankenskiestour on InstagramWE TALKED ABOUT...Trauma as psychological kindling that can inflame a fearful mentality, but can also illuminate the truthHow non-stop scary news keeps people locked in fight or flight modeThe big increase of untrue rumors spreading like wildfire through social mediaHas the COVID-19 been isolated and purified in a lab, to prove it's an infectious virus? Nope!David Crowe - The Infectious Myth & Coronavirus Panic (Research Paper) - LINKMedical Journal Article: A Novel Coronavirus from Patients With Pneumonia in China - LINKThe psychological impact on the elderlyFinding a way to offer our deepest humanity to othersThe effect of fear on our biology and healthAggressive treatment methods that are likely adding to the death count - LINKHow quarantine has limited activist resistance to 5G implementationThe Secure 5G And Beyond Act of 2020 - LINKThe biggest-ever mass exodus of insider CEOs in January 2020 - LINKEvent 201 - 2019's wargame simulation of a global coronavirus pandemic and economic meltdown - LINKNo proof that COVID-19 is caused by 5G, and why it's harmful to spread ideas without proofMaking the "end of the old world" be the time you create your own new worldThe reflection of our situation in the space weather (astrology)80% false positive rate with coronavirus Polymerase Chain Reaction testing - LinkDr Anthony Fauci (Faust?) predicts "surprise virus outbreak" during Trump Presidency - LINKHow our collective mental patterns create the big picture of societal behaviorWhy the "official" definition of what makes a confirmed case is what determines big or small statistics - LINKThe real reason the rate of confirmed cases in China have gone down (it's not because of quarantine)Over-sterilization dangers and hand sanitizers disrupting endocrine systems - LINKTiptoeing towards fascism, government seeking control over corporations - LINKAnonymous, Q-Anon, David Wilcock & Magical Savior Aliens--comfort culture and the problem with thinking insiders are going to secretly fix everything without us having to actWhy things don't go viral organically anymore, Facebook & Youtube censorshipThe lifting of the worldwide ban on bio-weapons research in 2015 - LINKDisputing the idea of infectious diseases based on real world exposure cases - LINKMaterialism as the foundational incorrect assumption at the bottom of our society's problemsThe Federal Reserve changes its capital reserve rules - LINKMore about the Federal Reserve: Century of Enslavement (Documentary by James Corbett) & The Creature From Jekyll Island (Book by G. Edward Griffin)The EARN IT act - attempting to illegalize online encryption & privacy - LINKHR 5717 and the government's desire to get rid of gun ownership in the US - LINKReal ID 2020 (LINK) and social credit scores in ChinaHow local and national media simply repeat the same information they're handed from aboveFrequency-Thinkers worth researching: Masaru Emoto (LINK), Nicola Tesla (LINK), Royal Rife (LINK), Wilhelm Reich (LINK)Alex Jones is Bill Hicks (LINK)The climate change alarmism build up to the coronavirus lockdown5G being installed around the country and in schools during quarantine when nobody is watching (LINK)The Handmaids Tale - 1990 movie revealing the plan to make humanity infertile (LINK)Spero Protection Clothing and being the hope (not hopium) the world needs (LINK)Pasco FL man surprised to see Florida announce his coronavirus death (LINK) SUPPORT INNERVERSE WITH SECRET ENERGY POWER-UPSBlessed Herbs Colon Cleanse Kit Shilajit - "The Destroyer of Weakness"Become a Secret Energy affiliate and generate Karma Free wealth - Link To Spiritech Specialist Program MUSIC IN THIS EPISODEIntro: "Foggy Dreams" by Wisdom TradersOutro: "Zombishh" by Lusid ft. Phi Lo See acast.com/privacy for privacy and opt-out information.

This episode covers the latest updates since our previous deep-dive on the novel coronavirus outbreak. We cover the latest developments -- new name, new declarations, new numbers, new definitions -- as well as:practical implications for the U.S. healthcare system given how it works today, and where we might go in the future -- with a16z general partner Julie Yoo, given our vantage point in tech; andhow the rt-PCR test works, and other updates about what the data do and don't tell us, and whether and what terms are useful or not -- with a16z bio partner Judy Savitskaya;...in conversation with Sonal Chokshi.Sources for this episode and/or other background links:latest numbers: cases in the U.S. (CDC, as of February 17, 2020); global cases (WHO situation report #25, February 14, 2020); spike in diagnosing cases as reported in China (SCMP, Scott Gottlieb)situation & policy statements/reports: CDC summary (as of February 14, 2020); "Annual report on global preparedness for health emergencies", WHO (Global Preparedness Monitoring Board, 2019)on definitions (of pandemics, endemics), other terms, and various naming conventions: "Understanding pandemics: What they mean, what they don't mean, and what comes next with the coronavirus", Helen Branswell, StatNews (February 12, 2020); on disease occurrence and levels (CDC); "misinfodemic"; best practices on naming new human infectious diseases (WHO); qPCR (Keith Robison)image: 2019 Novel Coronavirus (2019-nCoV), CDC, via Wikimedia Commons

Listen as we interview Becky Kucera, who was at the bench during the early years after the concept of the polymerase chain reaction (PCR) was conceived by Kary Mullis.

El programa de esta semana lo dedicamos a discutir varios temas relacionados con malaria a propósito del reporte de la Organización Mundial de la Salud correspondiente al 2017 (con datos del 2016) referente a este tema.   Referencias: WHO. World Malaria Report 2017 La malaria avanza sin frenos. Por Marielba Nunez. El Nacional. Nov 26, 2017. Polycarp Mogeni y colaboradores. Detecting Malaria Hotspots: A Comparison of Rapid Diagnostic Test, Microscopy, and Polymerase Chain Reaction. J Infect Dis. Nov 2017. Patricia Solis, Mariano Gustavo Zolis y colaboradores. Outbreak of human malaria caused by Plasmodium simium in the Atlantic Forest in Rio de Janeiro: a molecular epidemiological investigation  Lancet Glob Health 2017. A. Ashley y colaboradores. Spread of Artemisinin Resistance in Plasmodium falciparum Malaria. N Engl J Med. 2014 Mallika Imwong y colaboradores. The spread of artemisinin-resistant Plasmodiumfalciparumin the Greater Mekongs ubregion: amolecular epidemiology observational study. Lancet Infectious Dis. May 2017 Mallika Imwong y colaboradores. Spread of a single multidrug resistant malaria parasite lineage (PfPailin) to Vietnam. Lancet Infect Dis. Octubre 2017. WHO . Status Report on Aatemisinin and artemisinin-based combination therapy resistance. April 2017   La Frase de la Semana: La tomamos de Richard Phillips Feynman (11 de mayo de 1918 - 15 de febrero de 1988) fue un físico teórico estadounidense conocido por su trabajo en la formulación integral de ruta de la mecánica cuántica, la teoría de la electrodinámica cuántica, así como en la física de partículas. Ayudó en el desarrollo de la bomba atómica, amplió la comprensión de la electrodinámica cuántica, tradujo jeroglíficos mayas y e identifico la base del desastre del transbordador espacial Challenger. Por sus contribuciones al desarrollo de la electrodinámica cuántica, Feynman, , recibió el Premio Nobel de Física en 1965.  La frase dice asi: “La religión es la cultura de la fe; la ciencia es la cultura de la duda.”      

A teaching assistant explains the polymerase chain reaction (PCR), and examples of its common uses.

Interview with Joseph A. Califano, MD, author of Saliva and Plasma Quantitative Polymerase Chain Reaction–Based Detection and Surveillance of Human Papillomavirus–Related Head and Neck Cancer

Areas of discussion include Overview of nucleic acid amplification technologies, and Overview of Select Hospital Acquired Infections (Methicillin Resistant Staphylococcus Aureus, Vancomycin Resistant Enterococci, and Clostridium difficile).

At the end of the session, the participant will be able to 1) differentiate between PCR, TMA, SDA, and bDNA amplification technologies, 2) distinguish between end-point and real-time PCR, and 3) comprehend the impact on the laboratory of using an ASR versus an IVD product. Speaker: Nicole Robinson, Ph.D., Medical and Scientific Affairs, Roche Diagnostics Duration: 60 minutes Credit hours: 1.0

DNA / PCR-laboration

DNA / PCR-laboration

Mon, 12 Jul 2010 12:00:00 +0100 https://edoc.ub.uni-muenchen.de/11809/ https://edoc.ub.uni-muenchen.de/11809/1/Mayer_Veronika.pdf Mayer, Veronika ddc:550, ddc:500, Fakult

Sat, 1 Jan 1994 12:00:00 +0100 https://epub.ub.uni-muenchen.de/9394/1/9394.pdf Cremer, Thomas; Featherstone, Terry; Dunham, Ian; Lengauer, Christoph

Carcinoembryonic antigen (CEA) is a tumor marker that belongs to a family of closely related molecules with variable expression patterns. We have developed sets of oligonucleotide primers for the specific amplification of transcripts from individual CEA-family members using the reverse transcriptase/ polymerase chain reaction (RT/PCR). Specific primer sets were designed for CEA, non-specific cross-reacting antigen (NCA), biliary glycoprotein (BGP), carcinoembryonic antigen gene-family members 1, 6 and 7 (CGMI, CGM6 and CGM7), and one set for all pregnancy-specific glycoprotein (PSG) transcripts. Primers were first tested for their specificity against individual cDNA clones and product-hybridization with internal, transcript-specific oligonucleotides. Total RNA from 12 brain and 63 gynecological tumors were then tested for expression of CEA-related transcripts. None were found in tumors located in the brain, including various mesenchymal and neuro-epithelial tumors. CEA and NCA transcripts were, however, present in an adenocarcinoma located in the nasal sinuses. In ovarian mucinous adenocarcinomas, we always found co-expression of CEA and NCA transcripts, and occasionally BGP mRNA. CEA-related transcripts were also found in some serous, endometrioid and clear-cell ovarian carcinomas. CEA, NCA and BGP transcripts were present in endometrial carcinomas of the uterus and cervical carcinomas, whereas uterine leiomyomas were completely negative. No transcripts were found from CGM 1, CGM6, CGM7 or from PSG genes in any of the tumors tested. The PCR data were compared with immunohistochemical investigations of ovarian tumors at the protein level using CEA (26/3/13)-, NCA-50/90 (9A6FR) and NCA-95 (80H3)-specific monoclonal antibodies.

Fri, 1 Jan 1993 12:00:00 +0100 https://epub.ub.uni-muenchen.de/5984/1/Gerbes_ALexander_5984.pdf Nemer, M.; Gerbes, Alexander L.

Non-isotopic in situ hybridization of chromosome-specific alphoid DNA probes has become a potent tool in the study of numerical aberrations of specific human chromosomes at all stages of the cell cycle. In this paper, we describe approaches for the rapid generation of such probes using the polymerase chain reaction (PCR), and demonstrate their chromosome specificity by fluorescence in situ hybridization to normal human metaphase spreads and interphase nuclei. Oligonucleotide primers for conserved regions of the alpha satellite monomer were used to generate chromosome-specific DNA probes from somatic hybrid cells containing various human chromosomes, and from DNA libraries from sorted human chromosomes. Oligonucleotide primers for chromosome-specific regions of the alpha satellite monomer were used to generate specific DNA probes for the pericentromeric heterochromatin of human chromosomes 1, 6, 7, 17 and X directly from human genomic DNA.

We describe a new method for amplification, by polymerase chain reaction (PCR), of rearranged segments encoding the variable part of light and heavy chains of an antibody (Ab) from the chromosomal DNA of hybridoma cells for the chimerization ofAbs. A fundamental prerequisite for this is the knowledge ofthe exact sequences in the 5’-untranslated region of light and heavy chain mRNA, and of the joining segment used for rearrangement. This allows the design of nondegenerated oligodeoxyribonucleotides for PCR. The primer design permits directional cloning of the amplified, promoterless fragments into cassette vectors, in which they will be linked to the appropriate human constant domains and immunoglobulin (Ig) promoter/enhancer elements. The method is illustrated for chimerization of an Ab directed against the human T-lymphocyte antigen, CD4. The chimerized Ab is secreted in abundant quantities after transfection of the engineered plasmids into non-Ig-producing myeloma cells.