Podcast appearances and mentions of Kimberly Bryant

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Best podcasts about Kimberly Bryant

Latest podcast episodes about Kimberly Bryant

I Am Interchange
The Power of Feminine

I Am Interchange

Play Episode Listen Later Oct 30, 2023 70:06


Many would be surprised to realize that women only secured the right to vote in this country a little over a century ago, and that, perhaps tellingly, they began attending institutions of higher learning in remarkable numbers at about the same time. The female presence in business is further still in its infancy. Though women have outnumbered men in colleges and universities across the U.S. since the mid-90s, they remain an underrepresented minority in the workplace, particularly in positions and industries synonymous with success. But why? Is the problem merely timing? Is it men? Are the patriarchal systems and structures that have defined this nation simply intent on retaining that power, and intelligent in their methods of ensuring it? Or is it more complicated than that?  Here, on this journey through Egypt — guided by the wisdom and waters of the Nile — host Tate Chamberlin brings fellow HATCHers Meredith Marder, Catherine Carlton, and Kimberly Bryant together to share the perspectives and passions that have shaped their respective work as women in business, and their intentions for the generations of trailblazers that will follow in their footsteps.

Missions to Movements
The Magic Behind Scaling Black Girls Code with Kimberly Bryant

Missions to Movements

Play Episode Listen Later May 10, 2023 36:21


Kimberly Bryant started a movement out of a passion project. Black Girls Code has scaled significantly over the years and now has over 15 chapters across the United States and DREAM partnerships with brands like Google, TikTok, Verizon, the NBA, and Capital One.This inspiring conversation touches on so many pillars of growing a nonprofit organization from the ground up. You'll hear Kimberly's “founder's story”, her obsession with building their brand and making it pop, and how she scored all of those incredible brand partnerships for the long-haul. Plus, Kimberly shares the new trailblazing venture she's creating with Black Innovation Lab, a space to help marginalized founders and entrepreneurs build pathways to financial freedom and ownership.In This Episode:How the power of story, authenticity, and vulnerability helped Kimberly expand Black Girls Code so rapidly in the early daysKimberly's scrappy approach to creating their digital footprint, from leveraging volunteers and stock images, to pro-bono supportThe first role Kimberly hired within marketing and how she remained flexible to move people within the organization, especially if it was a better match for their talents and passionsHow Kimberly secured a multimillion dollar grant from Google to fund their New York headquarters (and how she ensures partnerships aren't just “one and done”)The moment where Kimberly saw an article on Twitter, which inspired her to turn an abandoned, historically Black college, into what will become the Black Innovation LabResources & LinksLearn more about Black Girls Code and follow Kimberly on Twitter and Instagram at @6gems. You can follow ASCEND Ventures Tech on LinkedIn to learn more about the Black Innovation Lab.What inspires website visitors to invest in your mission? Are you providing donors with a desirable online journey that keeps them coming back? Join my virtual session at the DonorPerfect Community Conference. I'll see you on June 6th and 7th! Applications for my Monthly Giving Mastermind program are now open. If you have been wanting to start a monthly giving program but haven't had the time or the team to make it happen, this is for you. We start in July, so apply now!Want to make Missions to Movements even better? Take a screenshot of this episode and share it on Instagram. Be sure to tag @positivequation so I can connect with you.  Let's Connect! Send a DM on Instagram or LinkedIn and let us know what you think of the show! Head to YouTube for helpful digital marketing how-to videos and podcast teasers Want to book Dana as a speaker for your event? Click here!

The Op-Ed Page with Elisa Camahort Page
Ep. 86: Why VC? Why Now?

The Op-Ed Page with Elisa Camahort Page

Play Episode Listen Later Mar 30, 2023 28:50


1. Why VC, why now? Kimberly Bryant twitter thread: https://twitter.com/6Gems/status/1640835647173951488 Kimberly Bryant's Linktr.ee: https://linktr.ee/ascendventures 2. Quick Takes: This Week-ish Newsletter re: SVB Collapse: https://elisacp.substack.com/p/financial-crisis-hits-close-to-home TikTok: My Top Book Launch Advice: https://www.tiktok.com/@elisacp/video/7215737194466643243 Funding campaigns: GOLD Comedy on IFundWomen: https://ifundwomen.com/projects/help-more-funny-women-make-more-funny-stuff The Cru on Republic: https://republic.com/the-cru Books: Tales of Potential by Joanna Bloor: https://www.talesofpotential.com/ The Anxious Achiever by Morra Aarons-Mele: https://morraam.com/books The Portfolio Life by Christina Wallace: https://www.penguin.co.uk/books/455973/the-portfolio-life-by-wallace-christina/9781529146349 Minimalista and Organized Living by Shira Gill: https://shiragill.com/books/ The Lit Lovers by Ciara Blume: https://ciarablume.com/ Where to find me: My website: https://elisacp.com Sign up for my new newsletter, This Week-ish with Elisa Camahort Page: https://elisacp.substack.com Calendly: Schedule a session with me!: https://calendly.com/elisacp Thanks to Ryan Cristopher for my podcast music: https://music.apple.com/us/artist/ryan-cristopher/1479898729 Road Map for Revolutionaries by me, Carolyn Gerin and Jamia Wilson: https://elisacp.com/books Social media handles: TikTok: @ElisaCP Mastodon: elisa@sfba.social Spoutible: @ElisaCP Twitter: @ElisaC Insta: @ElisaCP Please share, subscribe, rate and review!

MTG: More Than Graphics
The Ongoing March For Women In Tech With Tech Pioneer Kimberly Bryant

MTG: More Than Graphics

Play Episode Listen Later Mar 19, 2023 63:30


The march continues through #womenshistorymonth for #womenintech with #Technology pioneer #KimberlyBryant. Kimberly is a Memphis, Tenn., native, and always thought of herself as a “nerdy girl.” She loved math and science at an early age and it drove her passion to pursue a STEM career. Bryant was enticed by the early technology of microchips, personal computer and portable cell phones. She worked for numerous companies in the electrical, biotech and pharmaceutical fields such as Pfizer, Merck, Genentech and Novartis. Once Kimberly founded Black Girls Code, a nonprofit organization that focuses on providing technology and computer programming education to African-American girls, Bryant was listed as one of the "25 Most Influential African-Americans In Technology" by Business Insider. FOLLOW KIMBERLY: https://linktr.ee/ascendventures FOLLOW MTG: mtgthepodcast.com CLUBHOUSE TWITTER  CO-HOSTS: @octanedesigns / @bougienursebabe / @getsillycreative

The Pop Culture Green Room
Pop Culture-Loving Twin Sisters Walk Into A Room...

The Pop Culture Green Room

Play Episode Listen Later Feb 20, 2023 33:07


Join twin sisters Blair and Kimberly Bryant as they talk about what's on their minds this week in celebrity news and gossip with the latest on Armie Hammer, Kendall Jenner + Bad Bunny, a discussion of the first two episodes of Season 10 of Vanderpump Rules, and discussing their favorite products Sunga Life Apparel and Diptyque. Any questions, comments, inquiries, etc. email us at thepopculturegreenroom@gmail.com 1:18 - Armie Hammer 6:03 - Vanderpump Rules: Season 10 Episodes 1 + 2 Recap 21:09 - Kendall Jenner + Bad Bunny Dating? Tarot Card Reading 26:32 - Brands we're into: Sunga Life + Diptyque

UnapologeTECH
Expert Interview with Kimberly Bryant Founder and CEO Ascend Ventures | Founder and CEO The Black Innovation LABS | Founder Black Girls CODE

UnapologeTECH

Play Episode Listen Later Feb 10, 2023 48:49


Kimberly Bryant is the Founder and CEO of ASCEND Ventures and the Founder and creator of the groundbreaking and revolutionary non-profit organization Black Girls CODE; a social change organization founded to “change the face of technology” by introducing girls of color (ages 7-17) to the field of technology and computer science with a concentration on entrepreneurial concepts. Under Kimberly's leadership, Black Girls CODE grew from a local grassroots initiative serving only the Bay Area to an international organization reaching more than 30,000 students with chapters across the U.S. and in Johannesburg, South Africa. Kimberly has been nationally recognized as a thought leader for her work to increase opportunities for women and girls in the technology industry and has received numerous awards for her work with Black Girls CODE. Kimberly has been awarded the prestigious Jefferson Award for Community Service for her work to support communities in the Bay Area, named by Business Insider on its list of “The 25 Most Influential African-Americans in Technology”, and named to The Root 100 and the Ebony Power 100 lists in 2013. Kimberly has been named one of FastCompany's Most Creative People. Kimberly was named a White House as a Champion of Change for her work in tech inclusion and for her focus on bridging the digital divide for girls of color and received an Ingenuity Award in Social Progress from the Smithsonian Institute. Tune in today to hear Kimberly's Story as an African American Female Entrepreneur braking barriers and creating a better future for women of color in tech. --- Support this podcast: https://anchor.fm/unapologetech/support

Big Yellow School Bus Podcast
BYSB 11-9-22 A Worthy Cause and Educational Staffing Solutions (ESS)

Big Yellow School Bus Podcast

Play Episode Listen Later Nov 10, 2022 56:50


This episode introduces Brittany Ryan, physical therapist, and Lydia Fowler, registered dietician, and their new non-profit organization called "A Worthy Cause." The goal of this newly formed organization is to provide nutritional health and physical well-being education to elementary students and their families. They hope to accomplish this goal through providing interactive and supplemental activities in Maury County grade schools beginning sometime in the next school year. Also, Kimberly Bryant with Educational Staffing Solutions comes on as a guest to discuss the very pressing need for substitute teachers and the growing opportunities for interested applicants to become substitute teachers in Maury County.

Karen Hunter Show
Kimberly Bryant (Founder & CEO of Black Girls Code) & Azure Butler (Black Girls CODE alum and a political science major at Arizona State University, class of 2024)

Karen Hunter Show

Play Episode Listen Later Oct 11, 2022 35:10


BEHIND THE VELVET ROPE
Alexis Bellino - (on RHOC Past/Current/Future, Whether She Would Really Ever Return & "Jesus Jugs")

BEHIND THE VELVET ROPE

Play Episode Listen Later Sep 6, 2022 74:28


Alexis “Jesus Jugs” Bellino steps Behind The Rope. Today we share this never before released gem from the vault. Alexis takes us back to the beginning of her Real Housewives of Orange County journey, when then husband Jim brought her to the Season One Premiere Party and she met the then cast Vicki Gunvalson, Jo De La Rosa, Jeana Keough, Lauri Peterson and Kimberly Bryant. Fast forward several years, and Alexis was cast as a main RHOC cast member Season Five. The rest is history. Not so fast. Alexis opens up about how much life changed from when we saw her on RHOC as a married woman to Jim Bellino to her current life with love Andy Bohn. Alexis chats about the reasons for the demise of her marriage, the role, or lack thereof RHOC played in this demise, what it is like to now watch these scenes back all these years later, and what is was like to have the general public weigh in on your marriage. Alexis also chats about her time on the hit Bravo show and cast mates Tamra Judge, Heather Dubrow, Gretchen Rossi, and Vicki Gunvalson - who was the hardest to work with and the status of each of these relationships today. Alexis also discusses her Christian Faith and how that played out on the show. Yes, for all those wondering, we do discuss Alexis' moniker “Jesus Jugs” as bestowed upon her all those years ago by Tamra Judge. Speaking of Tamra, we DO bring up the Jim Bellino law suit against her and Shannon Storms Beador. Alexis also weighs in on the current cast and we finally answer why or why not she would really ever go back despite all the rumors out there.  @alexis_bellino @behindvelvetrope @davidyontef BROUGHT TO YOU BY: FREEDOMGROOMING - www.freedomgrooming.com/VELVET (20% Off Now) TALKSPACE - www.talkspace.com ($100 Off Your First Month. Use Code VELVET) RELIEFBAND - www.reliefband.com (20% off Plus Free Shipping. Use Code VELVET) ADVERTISING INQUIRIES - Please contact David@advertising-execs.com MERCH Available at - https://www.teepublic.com/stores/behind-the-velvet-rope?ref_id=13198 Learn more about your ad choices. Visit megaphone.fm/adchoices

Equity
Black Girls Code's developing story offers a complicated look at lots of different things

Equity

Play Episode Listen Later Aug 24, 2022 27:59


Hello and welcome back to Equity, a podcast about the business of startups, where we unpack the numbers and nuance behind the headlines.This is our Wednesday show, where we niche down to a single topic, think about a question and unpack the rest. This week, Natasha asked: What happens when a Black founder was ousted? Alex was back on the mic after a week away, and we brought on Dominic-Madori Davis (follow her on Twitter and Instagram) to talk us through the topic. After all, the core question was inspired by her latest TC+ column.Here's what we got into:The growing tensions at Black Girls Code, which began last December with Bryant's "indefinite suspension."Bryant's eventual ouster, which happened earlier this month, and the outpouring of public support for the leader. That conversation brought us into the reality of who gets to speak up publicly, and who can only do so off the record and without attribution.Davis piece about the symbolism of a Black founder being removed from a position of leadershipInherent bias and how to navigate that in the stories we hear and tips we receive.Equity drops every Monday at 7 a.m. PDT and Wednesday and Friday at 6 a.m. PDT, so subscribe to us on Apple Podcasts, Overcast, Spotify and all the casts.

The Heart of Giving Podcast
Creating Paths for Black Girls to Succeed in Tech

The Heart of Giving Podcast

Play Episode Listen Later Jul 26, 2022 47:41


In this week's episode, our guest is Kimberly Bryant, the Founder and CEO of Black Girls CODE, a non-profit organization dedicated to “changing the face of technology” by introducing girls of color (ages 7-17) to the field of technology and computer science with a concentration on entrepreneurial concepts. Have questions/comments/concerns? Email us at heartofgivingpod@gmail.com. Don't forget to subscribe and leave a comment on iTunes.

Testing 1, 2, 3
LANGUAGE with Kimberly Bryant and Eli Kerry

Testing 1, 2, 3

Play Episode Listen Later Jun 8, 2022 26:00


The lightning-fast pace of innovation brought on by the rapid advances in our testing technology owes at least part of its success to building, writing, and deploying a coding language that allows humans to follow and track it in a way that resembles how we think. This week, we explore how software-defined test has helped us improve and optimize our test, the differences between spoken language and coding, and the opportunities that arise when you are armed with a new vocabulary. To explore how we can better speak the language of test, host Derek Burrows welcomes Kimberly Bryant, founder and CEO of Black Girls Code, and NI's  Eli Kerry.  Learn More About: What is Kimberly's perspective on the differences between spoken language and coding language? What are the similarities? How has software-defined test helped us improve and organize in the automotive domain and beyond? While spoken languages may help us navigate and define the world around us, coding languages help us build them.Why was LabView created, and what does LabView code look like? With novel technologies like AI, we are teaching the computer how to solve problems sequentially by following a set of algorithmic steps, much like the teacher teaches their student. As our computers get smarter, will we see the line between our language and theirs start to blur?  Resources Mentioned: NIKimberly BryantBlack Girls Code Eli Kerry 

Living Corporate
Being a Founder While Black (w/ Kimberly Bryant)

Living Corporate

Play Episode Listen Later May 24, 2022 54:31


Zach sits down with Kimberly Bryant, founder and CEO of Black Girls CODE, to talk about her journey, lessons learned, and advice for other Black and brown founders in and outside of tech. Connect with Kimberly on LinkedIn & Twitter. https://bit.ly/3wOxDdo https://bit.ly/3lIWIBn Interested in learning more about Black Girls CODE? Check out the website. https://bit.ly/3yPehHY Interested in supporting Living Corporate? Check out our Support page. https://bit.ly/3egO3Dk

Mental Toughness Mastery Podcast with Sheryl Kline, M.A. CHPC
Building and Supporting an Equitable Talent Pipeline - an Interview with Kimberly Bryant

Mental Toughness Mastery Podcast with Sheryl Kline, M.A. CHPC

Play Episode Listen Later Apr 22, 2022 24:33


http://www.sherylkline.com/blogIn case you missed my LIVE interview with the remarkable Kimberly Bryant (Founder and CEO of Black Girls Code), here are a few of the wisdom gems she shared:  Understand what your core values are, and align yourself and all facets of life with folks that share those values.Being a good student will allow you to be exposed to opportunities.Women are valuable and worthy of exploring their pathways and dreams.Continue on the pathway of growing internally.Understand that the world is changing at a very rapid pace.If we don't evolve as leaders in a way to keep up with that rapid change of growth, we won't be very effective.To be notified for future interviews be sure to connect with me here on LinkedIn (https://www.linkedin.com/in/sherylklinema/) AND to learn more about how to build the mindset and influence for real change, join our community at www.SherylKline.com. #limitlessleader​​ #womenwholead​​ #sherylkline​​ #blackgirlscode #equality

This Is Working with Daniel Roth
Kimberly Bryant on what's next for her and Black Girls CODE

This Is Working with Daniel Roth

Play Episode Listen Later Apr 14, 2022 34:47


Kimberly Bryant spent decades building up her career as an engineer in corporate America – until she decided to leave it all behind to pursue her true passion. In 2011, she drained her retirement savings to launch Black Girls CODE, a non-profit focused on building community while teaching technology skills to Black school-age girls. Today, Black Girls CODE has trained nearly 20,000 girls and is a $40 million powerhouse whose backers include Google, MacKenzie Scott, and the Gates Foundation. In this conversation with LinkedIn Editor-in-Chief Daniel Roth, Bryant discusses her inspiration for the non-profit, the challenges she faced as its leader, and her surprise suspension from the organization she founded. Follow Kimberly Bryant on LinkedIn Follow Daniel Roth on LinkedIn Join our This is Working community by subscribing to Dan's newsletter and posting your thoughts using the hashtag #ThisIsWorking.

I Am Interchange
Rethinking Philanthropy

I Am Interchange

Play Episode Listen Later Mar 8, 2022 38:50


In this podcast, our host Tate Chamberlin reimagines philanthropy with HATCH Rethinking Philanthropy lab facilitator Ada Williams Prince and esteemed lab guests, Asiaha Butler and Kimberly Bryant. Ada Williams Prince is the senior advisor for program strategy and investment at Pivotal Ventures, where she works to accelerate positive social programming and evolution, particularly in the areas of adolescent mental health and empowerment and access for women and girls of color. Former electrical engineer and current founder and CEO of Black Girls CODE, a non-profit that introduces girls of color to technology and computer science, Kimberly Bryant has grown her grassroots initiative since 2011 to reach over 30,000 young women worldwide. And Asiaha Butler, co-founder and CEO of the Resident Association of Greater Englewood or R.A.G.E. in Chicago, works to reinvigorate the greater Englewood neighborhood by mobilizing “residents and resources to force a change in the community.” Through the voices and experiences of these commanding women of color, this podcast provides a glimpse of the struggle, the passion, and the power behind change and its makers.

Techish
Open Sea worth $13B! Elizabeth Holmes Going Jail? Drama at "Black Girls Code", NFT's

Techish

Play Episode Listen Later Jan 13, 2022 35:26


Techish is back with a brand new bonus episode! Abadesi and Michael, break down:- The Molly Mae controversy that has been dominating the headlines O.37- Elizabeth Holmes: Where is the former Theranos CEO now?  10.39- Is the media doing its due diligence on founders and whether their businesses actually work? - 15.21- NFT marketplace OpenSea is now worth $13B+ 19.50- Black Girls Code founder Kimberly Bryant was suspended from her own organization 26.09- Career Karma founder Ruben Harris raises $40M in Seed B - 32.52—————————————————————www.pocitjobs.com for roles for POC in techwww.hustlecrew.co for talks and training to make your company more inclusive—————————————————————Extra Reading:https://www.standard.co.uk/news/uk/molly-mae-hague-apology-podcast-the-diary-of-a-ceo-steven-bartlett-b975901.htmlhttps://techcrunch.com/2022/01/08/theranos-elizabeth-holmes-startup-friends/https://techcrunch.com/2022/01/04/nft-kingpin-opensea-lands-13-3b-valuation-in-300m-raise-from-paradigm-and-coatue/https://peopleofcolorintech.com/break-into-tech/update-black-girls-code-ceo-releases-statement-and-denies-allegations-of-work-place-impropriety/https://peopleofcolorintech.com/front/imposter-syndrome-believing-youre-enough-and-raising-40m-founder-ruben-harris-tells-all/————————————————————Use the hashtag #Techish on Twitter & IGSupport Techish at www.patreon.com/techishAdvertise on Techish: goo.gl/forms/MY0F79gkRG6Jp8dJ2————————————————————Stay In Touch:www.twitter.com/michaelberhane_www.twitter.com/abadesiwww.twitter.com/hustlecrewlivetwitter.com/techishpodEmail us at techishpod@gmail.com

#RolandMartinUnfiltered
Biden-Harris & Voting Rights, Black Girl Code CEO out, Black Virtual Mall, LA's Black Female Sheriff

#RolandMartinUnfiltered

Play Episode Listen Later Jan 12, 2022 117:12


1.11.2022 #RolandMartinUnfiltered: Biden-Harris & Voting Rights, Black Girl Code CEO on leave, Black Virtual Mall, LA 1st Black Female Sheriff President Joe Biden and Vice President Kamala Harris take a trip to the epicenter of the American civil rights, Atlanta. Today, they paid their respects to Martin Luther King, Jr. before speaking at the Atlanta University Center Consortium on the urgency to pass voting rights legislation and protect Americans' sacred right to vote. Georgia Congressman David Scott is here to help us break down what the president said. And a coalition of twenty-five faith leaders from across the nation has been on a hunger strike since the first anniversary of the insurrection to urge Congress to protect democracy by passing voting rights legislation by Martin L. King, Jr. Day. Three of them are here with us tonight. We now know the name of the white off-duty police officer who gunned down a black man who he claims jumped on his truck. We'll have the latest from North Carolina. She was suspended from the organization she founded. Tonight, I'm talking to Kimberly Bryant from the Oakland-based tech nonprofit, Black Girls Code about the suspension and the investigation. Plus, she's making history in Louisiana by being elected the first black female sheriff in the state's history. We will be talking to Orleans Parish Sheriff-Elect Susan Hutson about her plans for the parish. And in our Marketplace segment. She made going to the mall easier, and you don't even have to leave your home. The mastermind behind the first-ever Black Virtual Mall will explain how it works. #RolandMartinUnfiltered partners: Verizon | Verizon 5G Ultra Wideband, now available in 50+ cities, is the fastest 5G in the world.* That means that downloads that used to take minutes now take seconds.

TechCrunch
Daily Crunch 12/29/21

TechCrunch

Play Episode Listen Later Dec 29, 2021 2:33


More companies bow out of in-person CES presence; Kimberly Bryant's suspension surfaces ongoing tensions at Black Girls Code; Religious platforms draw investor attention

TechCrunch
Daily Crunch 12/29/21

TechCrunch

Play Episode Listen Later Dec 29, 2021 2:33


More companies bow out of in-person CES presence; Kimberly Bryant's suspension surfaces ongoing tensions at Black Girls Code; Religious platforms draw investor attention

REVOLT BLACK NEWS
Crime, Cryptocurrency, and The Revolution To Confront Colonialism

REVOLT BLACK NEWS

Play Episode Listen Later Dec 10, 2021 44:04


In this episode of REVOLT Black News Weekly, Eboni K Williams and panel examine the 500+ homicides in Philly, making it the deadliest year in the city's history. Philadelphia Councilman Kenyatta Johnson joins to answer the question - what is going on in Wild West Philly?Host of Maconomics, Ro$$ Mac and Personal Finance Expert Ash Cash are Guests in the roundtable that break down how to get into the game of digital currency and avoid the pitfalls!RBN Special Correspondent Kennedy Rue McCullough takes us through this week's REVOLT Radar which includes Drake withdrawing his 2 Grammy nominations, Jussie Smollett takes the stand, and Meagan Good stops by to discuss her new Amazon show, ‘Harlem.'RBN Special Correspondent Neima Abdulahi moderates a discussion on Barbados breaking free from Buckinham palace. Guests Kobie Broomes, Kareem Smith and Dr. Claire Nelson join to dissect what this means for the island and how Rihanna was declared a national hero.  And in another edition of REVOLT's ‘Stand Up For' Series presented by State Farm, the non-profit Black Girls Code spotlights “Black Innovation.” Its CEO, Kimberly Bryant drops by to highlight their vital work in the Bay Area where they're changing the face of technology!

Housewives Heretics

We enter the gates of Orange County and meet 5 luncheon loving, sky top wearing women and their families. Join us where it all began as we reintroduce ourselves to Kimberly Bryant, Jo De La Rosa, Vicki Gunvalson, Jeana Keough, and Lauri Waring.

The Wonder Women Tech Show
Becoming Undaunted on the Road to Success with Kara Goldin, Founder and CEO of Hint, Inc.

The Wonder Women Tech Show

Play Episode Listen Later May 18, 2021 70:40


Our season 1 finale guest is the extraordinary Kara Goldin! Before becoming a bestselling author and the Founder and CEO of Hint, Inc., Kara was already an incredibly successful business woman working for companies like AOL, Time Inc., and CNN. Never taking "No" for an answer, Kara is determined to carve out her life on her own terms. Look for Kara's inspiring book, Undaunted: Overcoming Doubts and Doubters, in stores now! Listen in as Kara and Lisa Mae Brunson - host of the podcast and Founder of Wonder Women Tech - discuss Kara's vast career, what it takes to become a CEO, and why you should never, ever, doubt yourself. Today's pioneering woman is Kimberly Bryant, an electrical engineer who founded Black Girls Code, a nonprofit that teaches STEM skills to young Black girls. Bryant has been named one of the 25 most influential African Americans in technology by Business Insider. Thank you for your pioneering contributions, Kimberly Bryant. An enormous thank you to all of our listeners for making our first season a success! Please be sure to Rate and Review this and all our podcast episodes! You can connect with @wonderwomentech on Twitter, Facebook, Instagram and Linked In! Learn more about our story and mission at https://wonderwomentech.com/ (wonderwomentech.com) Sound Engineering and Music by Carleigh Strange Art Work By Jessenia Hernandez - @sen.i.a on Instagram

Behind The Tech with Kevin Scott
Kimberly Bryant: CEO & Founder of Black Girls Code

Behind The Tech with Kevin Scott

Play Episode Listen Later Apr 27, 2021 62:43


This charismatic leader talks about untapped potential – and why encouraging young people to study computer science is critical for the future of technology. Find out how Kimberly’s efforts are impacting STEM initiatives across the U.S. Click here for transcript of this episode.  Kevin Scott BlackGirlsCode

Intersectional Insights
Fragility As A Defense, and Male Fragility

Intersectional Insights

Play Episode Listen Later Mar 19, 2021 32:20


Olivia and Raven discuss fragility as a defense, linking personhood to ideas, male fragility, and embracing vulnerability. Note: There's low quality audio on Raven's end until 01:16. Discussion Summary: 0:33: We're talking about fragility as a defense, equating ideas with identity, and resistance to consciously adjusting one's understanding when presented with new information. 04:59: Ideas and standards change over time, and a digression about different areas of the world using different calendars. 07:19: Linking fear of change to hating being wrong, pride paired with intelligence and knowledge, and people feeling attacked when someone points out that they're wrong. 11:04: Male fragility, male entitlement, the one-dimensional view of what it means to be a man, and failing to recognize strengths in women. 14:05: Expanding upon what it means to provide and protect as a partner, men feeling emasculated because women's roles have expanded, and sharing roles and responsibilities doesn't lessen your self-worth. 20:37: emotional fragility in men, the one-dimensional view of vulnerable, and emotional vulnerability as a strength. 26:18: The tough guy persona as a form of male fragility, certain ways of being emotional are viewed positively when expressed by men, 30:59: Black beauty highlight: Kimberly Bryant. 31:46: Outro.   Social media: Twitter @I_squaredpod, our Facebook page, and our public Facebook group, Intersectional Insights. Music credits: Opening: Goestories - Noir Et Blanc Vie Black Beauty Highlight background music: "Bass Walker" Kevin MacLeod (incompetech.com) Closing: First Class - DJ Williams

EdTech Today
How SXSW EDU Lives On, Online

EdTech Today

Play Episode Listen Later Mar 5, 2021 26:13


In this episode of EdTech Today, SXSW EDU executive producers Ron Reed and Greg Rosenbaum detail their pivots this past year and share valuable insights for any ed exec who creates, exhibits, or attends edtech events.Keynote sessions convene the SXSW EDU Online community each morning, bringing together powerful voices for discourse on a topic impacting education. This year, the keynote lineup spotlights incredibly timely and important discussions for all education stakeholders.Commencing SXSW EDU Online, as previously announced, Oprah Winfrey and Dr. Bruce Perry will lead the opening keynote discussion on the impact of childhood trauma on who we become, the decisions we make, and how healing must start with one question ‘what happened to you?' Ms. Winfrey and Dr. Perry have co-authored a book on the topic, What Happened to You?: Conversations on Trauma, Resilience, and Healing, which will be released in April.Newly announced, Kimberly Bryant, Founder and CEO of Black Girls CODE and Reshma Saujani, Founder and CEO of Girls Who Code will be in conversation for Wednesday's keynote discussion on the gender gap in computing and programming, especially in underrepresented groups.On the final day of SXSW EDU Online programming, author Nic Stone and Brittany Hogan, Director of Educational Equity and Diversity at Rockwood School District, will give their individual and collective thoughts and ideas about how diverse literature can change the hearts and minds of students in the keynote, Empathy in Equity: Author and Educator. Join the keynote speakers directly after their session for a live Q&A you won't want to miss.Join Us for SXSW EDU OnlineSecure your pass for SXSW EDU Online, March 9-11, 2021 at the current rate of $139 before passes sell out. SXSW EDU Online also offers group and student discounts.Sign up for the SXSW EDU email update for all the latest news and be sure to follow us on Twitter, Facebook, LinkedIn and Instagram for the latest news and updates.

Black Hypothesis
Celebrating Black Engineering Week!

Black Hypothesis

Play Episode Listen Later Feb 22, 2021 34:57


February 21-27 is Engineering Week, but it's also Black History Month. So we decided to remix it and celebrate Black Engineering Week! In this episode, we talk about the incredible feats of these 10 amazing Black engineers: Mary Jackson, Walt Braithwaite, Ursula Burns, William Hunter Dammond, Wanda M. Austin, Lewis Latimer, Jerry Lawson, Mark Dean, Kimberly Bryant, and Mae Carol Jemison.Support the show (https://paypal.me/blackhypothesis?locale.x=en_US)

The Aging Athlete
#20 Interview with Physical Therapist Specializing in Pelvic Floor - Kimberly Bryant, DPT

The Aging Athlete

Play Episode Listen Later Dec 27, 2020 38:27


What an interesting interview with physical therapist and pelvic floor health specialist, Dr. Kimberly Bryant.  Kim's areas of expertise include prevention and wellness, pelvic pain, low back pain, prolapse (pelvic pressure), bowel health, pain with intercourse, incontinence, painful menstrual cycles, and prenatal/postpartum care. Her goal is to empower women to take their wellness into their own hands as they discover their personal healing path.  This was not a specialty back when I had children and I think even though this is an area that is now more well known, many women still haven't heard of this or know that this is available.  Even lower back issues can be traced to pelvic issues so be sure to reach out to Kim or someone with her training to help you - no matter your age (older or younger) because many times problems happen long after having children and not necessarily immediately post-partum.   Dr. Bryant earned her Doctorate in Physical Therapy from A.T. Still University in Mesa, AZ in 2009. She opened her private practice, Thrive Pelvic Health and Wellness in 2017 to provide holistic rehabilitation and wellness care for women with a focus on pregnancy and postpartum support. She provides in person and tele-health treatment sessions.  When away from the office, Kim enjoys spending time with her two young boys, husband, and running out on the AZ mountain trails with friends. Website:  https://thrivepelvichealth.com/Private FB Group:  https://www.facebook.com/groups/440651889879639To Follow Her on Instagram:  https://www.instagram.com/thrivepelvichealth/

TV. Watch. Repeat.
The Real Housewives of Orange County

TV. Watch. Repeat.

Play Episode Listen Later Nov 30, 2020 68:14


In the debut episode of TV. Watch. Repeat., The Dipp's Allison Piwowarski and Kate Ward dig into the pilot that launched a behemoth franchise, Bravo's The Real Housewives of Orange County. Come for the skytops, stay for the story behind what might just be considered television's first hate watch. Plus, discover just how much the show — and its Season 1 stars Vicki Gunvalson, Kimberly Bryant, Jeana Keough, Lauri Waring, Jo De La Rosa, and Slade (the sixth Housewife, of course) — have changed since its 2006 premiere.

WSJ Tech News Briefing
Tech Live: Bridging the Digital Divide and the Road to Tech Equity

WSJ Tech News Briefing

Play Episode Listen Later Oct 23, 2020 23:07


The pandemic has exacerbated the digital divide: who has access to the internet, and who doesn't. On today's episode, FCC Commissioner Jessica Rosenworcel and Black Girls Code Founder and CEO Kimberly Bryant discuss how the public and private sectors can work together to democratize access to tech. Chief Economics Commentator Greg Ip moderates. Amanda Lewellyn hosts. Production assistance from Trenae Nuri. Kateri Jochum is the executive producer of WSJ Podcasts. Learn more about your ad choices. Visit megaphone.fm/adchoices

equity production bridging digital divide tech live kimberly bryant fcc commissioner jessica rosenworcel kateri jochum
EdQuarter: The Education Station Podcast
Kimberly Bryant, Founder & CEO of Black Girls CODE

EdQuarter: The Education Station Podcast

Play Episode Listen Later Oct 18, 2020 28:58


Kimberly Bryant is the founder and CEO of Black Girls CODE – a non-profit organisation dedicated to “changing the face of technology” by introducing girls of colour (ages 7-17) to the field of technology and computer science with a concentration on entrepreneurial concepts. Prior to starting Black Girls CODE, Kimberly enjoyed a successful 20+ year professional career in the pharmaceutical and biotech industries as an engineering manager in a series of technical leadership roles for various Fortune 50 companies such as Genentech, Merck, and Pfizer. Since 2011, Kimberly has helped Black Girls CODE grow from a local grassroots initiative serving only the Bay Area, to an international organisation with fourteen chapters across the U.S. and in Johannesburg, South Africa. Black Girls CODE has currently reached over 10,000 students and continues to grow and thrive. Kimberly has been awarded the prestigious Jefferson Award for Community Service for her work to support communities in the Bay Area, named by Business Insider on its list of ‘The 25 Most Influential African-Americans in Technology’, and featured on The Root 100 and the Ebony Power 100 lists in 2013. Kimberly has been deemed one of FastCompany’s Most Creative People, and was named a White House as a Champion of Change for her work in tech inclusion, as well as for her focus on bridging the digital divide for girls of colour. In this exclusive one-to-one interview, Anna Britten – managing editor of EdQuarter – explores Kimberly’s journey from engineering manager to edtech pioneer. They will discuss Kimberly’s experience as a woman of colour in tech, her campaign for increased diversity and her continuing battle for BAME inclusion.

Have a Blessed Gay
Ep. 21 All Are Welcome (Not Really): How to be active in a church that doesn’t accept you

Have a Blessed Gay

Play Episode Listen Later Sep 29, 2020 39:44


Welcome to Have a Blessed Gay, your weekly spiritual-comedy podcast! Join holy host, Tyler Martin, as he critically discusses all things to do with religion and spirituality from an outcast's perspective. If you search online forums regarding women in religion or the LGBTQ+ community in religion, you’ll find a lot people asking questions like, “How can I be active in a church that doesn’t accept me?” This is a very common thread in many marginalized groups, not strictly within Christianity, but within most main world religions. Holy Host, Tyler Martin, examines why someone might participate or want to be involved in a church that is not affirming. To explore these topics, he looks at the megachurch Hillsong and Chick-Fil-A. Sometimes, like much of our country’s discriminatory legislation, bigotry and hate is hidden behind flowery language and the word “welcome.” This is also an exciting episode because The B Word launches today, a new vertical on the Have a Blessed Gay website for outcasts to share their journeys, ideas, questions, and resources, all to do with religion and spirituality. You’ll be able to read articles from people of various religions, faiths and spiritual walks of life, who are questioning, daring to be different, and making positive changes in their communities. Toward the end of this episode, you’ll hear an excerpt from the very first article, Reclaiming the Divine Feminine Voice, by the author herself, Kimberly Bryant. The full article is now available on haveablessedgay.com/thebword. Read it and subscribe to the newsletter to know when new articles drop! The B Word www.haveablessedgay.com/thebword Reclaiming the Divine Feminine Voice by Kimberly Bryant Sponsored by BetterHelp! Get 10% Off BetterHelp therapy by using my code! www.betterhelp.com/blessedgay Your Host: Tyler Martin Instagram: @tylerisaacmartin Follow Have a Blessed Gay and Reach Out! Instagram/Twitter/Facebook: @haveablessedgay www.haveablessedgay.com Email: hello@haveablessedgay.com U.S. Helplines National Suicide Prevention Lifeline: 1-800-273-8255 Trevor Project Helpline (LGBTQ+ Youth): 1-866-488-7386 Trans Helpline: 877-565-8860

SXSW Sessions
Black Girls CODE: Securing the Future for Black Women in Tech

SXSW Sessions

Play Episode Listen Later Jun 8, 2020 53:56


Led by tech pioneer Kimberly Bryant, Black Girls CODE is an organization dedicated to bringing girls of color into the field of technology as transformational change agents. In this session, Bryant leads a panel featuring BGC students and alumni under the age of 20 for a lively discussion on education, social activism, and securing the future for black women in technology, while encouraging a broader conversation on how intersectionality can create more opportunities for girls of color in STEM and drive social change movements.

Seamless Podcast with Darin Andersen
Kimberly Bryant, KidWonder | Seamless Podcast: GigaRaise Ready, Set, Go!

Seamless Podcast with Darin Andersen

Play Episode Listen Later Feb 20, 2020 21:40


Darin and Mike welcome Kimberly Bryant, CEO and Founder of KidWonder, San Diego's first kinetic learning academy. Kim shares experiences from her childhood and the role sports participation played in her overall development and why it's so important for kids to learn through playing. Kim explains how kinetic learning can allow children to actively grow their confidence while exploring new and fun activities, such as the American Ninja Warrior Junior competition taking place at KidWonder in April. 

The Good News Podcast
Wiki Women: Kimberly Bryant

The Good News Podcast

Play Episode Listen Later Sep 20, 2019 3:01


Kimberly Bryant is an African American electrical engineer. Byant founded Black Girls Code, in 2011. Black Girls Code is a training course that teaches basic programming concepts to black girls who are underrepresented in technology careers. Additionally, the organization offers programs in computer programming, coding, as well as website, robot, and mobile application-building. African-American women make up less than 3% of the workforce in the tech industry and Black Girls Code fights to change and improve this percentage for the better- BGC has a major goal of teaching 1 million girls of color to code by 2040.In 2014, Bryant was the recipient of Smithsonian Magazine's American Ingenuity Award for Social Progress.As of this recording, BGC has reached more than 8,000 young women in 15 chapters around the world! Interested? What to learn more? Know a little who is interested in STEM. Check out http://www.blackgirlscode.com/★ Support this podcast on Patreon ★

Hack the Process: Mindful Action on Your Plans
Contemporary Coworking, Prolific Podcasting, Subscriber Segmentation, and More

Hack the Process: Mindful Action on Your Plans

Play Episode Listen Later Aug 1, 2019 4:12


Contemporary Coworking, Prolific Podcasting, Subscriber Segmentation, and More in Process Hacker News Welcome to the Process Hacker News, your weekly roundup of useful news and updates from Process Hackers who have been guests on Hack the Process with M. David Green. This week we’ve got contemporary coworking, prolific podcasting, subscriber segmentation, and more. For all the links, check out the show notes at https://www.hacktheprocess.com/contemporary-coworking-prolific-podcasting-subscriber-segmentation-and-more-in-process-hacker-news/. Enjoy! Events Catch Jenny Feinberg in the Castro Art Walk in San Francisco, as she showcases her vibrant, expressive paintings on August 1. Seerfest 2019 is a one-day digital marketing event on October 3 in Philadelphia, where April Dunford will be a speaker. All sales from the tickets will go to Hopeworks Camden, an organization set up to help the youth of Camden, New Jersey achieve technology skills and job opportunities. Media In a world where working remotely at home is starting to be more common, why is coworking still valuable? You’ll hear about it in an interview with Alex Hillman on the Building Remote Teams Podcast. On the Inspired Money Podcast, you can find out more about Engel Jones and how he launched his record-setting podcast with the goal of having thousands of meaningful conversations with folks. Bill Wooditch pays a visit to the Breakfast Leadership Podcast to discuss his book, Fail More, and how failures can be used as foundations. In an interview with Bloomberg, Safi Bahcall discusses why he thinks Richard Branson and Elon Musk have outdone NASA. Writing Why do you need to segment your subscriber list and what’s the best way to do it? Brennan Dunn has come up with a beginner’s guide to help you out. When your boss betrays you it can change our career outlook, and most times, it’s for the worse. In a new HBR article, Ron Carucci offers ways to avoid the risk of becoming someone you don’t want to be. Some folks still prefer physical books over ebooks, and Curtis McHale is one of them. He also names a few book titles important enough to buy in hard copy. Recommended Resources Sean Ellis, who was referenced by Vinay Patankar and Maneesh Sethi, is working with Itamar Gilad to present the Breakout Growth Workshop in Barcelona on October 1. The event hopes to accelerate value delivery and achieve breakout growth for both new startups and established companies. On October 3, TEDxSan Francisco will be featuring a wide range of speakers, including Lauren Kunze, who was mentioned by Loic Le Meur, and Kimberly Bryant, who inspired Michelle Kim. Here is a list of interesting UX folks you can follow on Twitter, and one of them is Laura Klein, who was recommended by Rich Mironov in his Hack the Process interview. Thanks for checking out this Process Hacker News update from Hack the Process. If you liked what you saw, please leave a comment to let us know what processes you’re hacking.

Revision Path
Welcome to Revision Path!

Revision Path

Play Episode Listen Later Jul 25, 2019 1:21


This is Revision Path, an award-winning weekly interview podcast that showcases the world's best Black designers, developers, and digital creatives. If you're looking to get inspired, then tune in each week for in-depth conversations that explore the creative journey, including the processes, thoughts, and motivations behind these awesome creators shaping the future of art, design, and technology. Some past guests include Kimberly Bryant (founder and executive director of Black Girls Code), Eddie Opara (partner at Pentagram), Fahamu Pecou (international artist and scholar), Gail Anderson (National Design Award winner), and Hannah Beachler (Academy Award winning production designer). Whether you're a product designer, web designer, software developer, or just someone who works in the tech, design or creative industries, then this podcast is for you! New episodes of Revision Path are available every Monday! We'll see you then!

Hack the Process: Mindful Action on Your Plans
Chaos, Segmentation, Impossible Goals, and More in Process Hacker News

Hack the Process: Mindful Action on Your Plans

Play Episode Listen Later May 14, 2019 4:35


Welcome to the Process Hacker News, your weekly roundup of useful news and updates from Process Hackers who have been guests on Hack the Process with M. David Green. This week we’ve got chaos, segmentation, impossible goals, and more. For all the links, check out the show notes at https://www.hacktheprocess.com/chaos-segmentation-impossible-goals-and-more-in-process-hacker-news/. Enjoy! Events You can catch Brian Solis, our latest guest on Hack the Process, in person at the free event Open Influence Summit 2019 in Los Angeles on May 15, and at SVB + Create33 Author Series in Seattle on May 16. The first Forbes Opportunity Zones Summit: Investing for Impact is on May 21 in Newark, New Jersey. Catch Anne Driscoll discussing how her coworking startup, Launchpad, is helping entrepreneurs in locations that need it most. Engineering leaders such as Ron Lichty will be presenting at the 2019 San Francisco CTO Summit on May 21 to share their wisdom about better processes and more effective management. Media Alex Cespedes has rebranded his podcast “Or So I Thought.” Listen to the inaugural episode, inspired by Andy Warhol and Bill Murray, as Alex shares his musings on indirectly accomplishing goals. Are you stumped by Facebook Ads and how they work? Learn a few basics from the author of Facebook Ads Manual and founder of Super Spicy Media, Mojca Zove, as she reveals why Facebook Ads can be a good investment, and how they work compared to other social media platforms, on the In the Trenches Podcast with Tom Morkes. Thuy Pham is the founder of Freshly Baked Brand, through which she helps clients with their brand strategies. Hear how she crafts memorable brands in her recent conversation with Engel Jones on the Twelve-Minute Convos Podcast. Marketers can learn the three segmentation essentials they need to know from this video taught by Brennan Dunn. Writing The winners of the 2019 Independent Press Awards have been announced, including Shackled, the biography book written by Adam Siddiq. Congratulations, Adam! Loonshots, a term coined by Safi Bahcall are big ideas or goals that seems impossible. In a Q&A with AEI, Safi talks about the wisdom of nurturing loonshots and how it’s been proven out over and over in history. Recommended Resources Everyday Chaos is a book by David Weinberger coming out on May 14, about how we’re living with technology in today’s complex world. Tara Hunt recommended David during her Hack the Process interview. Let your girls 7-12 explore webmaking at Black Girls Code, Seattle’s Build a Webpage in a Day in Tacoma, Washington on June 15. Kimberly Bryant, founder of Black Girls Code, was a source of inspiration for Michelle Kim. The CMO Podcast was recently launched by Jim Stengel, who was mentioned by Steve Goldbach, and the first episode tackles the mindset of a Chief Marketing Officer. Award-winning author and psychologist Dr. Shefali Tsabary, recommended by Heather Chauvin, breaks down the meaning of great parenting in this fireside chat with Mindvalley’s Vishen Lakhiani, who was suggested by another Hack the Process guest, Michelle Dale. Thanks for checking out this Process Hacker News update from Hack the Process. If you liked what you saw, please leave a comment to let us know what processes you’re hacking.

Politics and Polls
#133: Women of Color in the Digital Space Ft. Kimberly Bryant

Politics and Polls

Play Episode Listen Later Apr 4, 2019 32:34


The technology sector is an important part of the economy, yet there exists a dearth of women in the field — especially females of color. Joining this episode to discuss breaking through these barriers is Kimberly Bryant, founder and CEO of Black Girls CODE, a non-profit organization dedicated to “changing the face of technology” by introducing girls of color (ages 7-17) to the field of technology and computer science. Bryant’s organization will host a workshop for young girls who want to explore artificial intelligence this Saturday, April 6, in New York City. Prior to starting Black Girls CODE, Bryant worked for over 20 years in the pharmaceutical and biotech industries as an engineering manager in a series of technical leadership roles for various Fortune 50 companies such as Genentech, Merck, and Pfizer. Since 2011, Bryant has helped Black Girls CODE grow from a local grassroots initiative serving only the San Francisco Bay Area, to an international organization with fourteen chapters across the United States and in Johannesburg, South Africa. Black Girls CODE has currently reached over 7,000 students and continues to grow and thrive.

Everyday Black History: Afro Appreciation
Kimberly Bryant- electrical engineer and technologist who founded Black Girls Code.

Everyday Black History: Afro Appreciation

Play Episode Listen Later Feb 20, 2019 6:54


Kimberly Bryant is an electrical engineer and technologist who worked at several companies in the biotech and pharmaceutical fields. She is known for establishing Black Girls Code which teaches basic computer programming to young girls to increase the percentage of Black women in the tech fields. Enjoy --- This episode is sponsored by · Anchor: The easiest way to make a podcast. https://anchor.fm/app --- Send in a voice message: https://anchor.fm/EverydayBlackHistory/message Support this podcast: https://anchor.fm/EverydayBlackHistory/support

Hack the Process: Mindful Action on Your Plans
2018-12-04 Process Hacker News

Hack the Process: Mindful Action on Your Plans

Play Episode Listen Later Dec 4, 2018 4:11


Holiday Mentors, Profitable Podcasts, Trauma Therapy, and More Welcome to the Process Hacker News, your weekly roundup of useful news and updates from Process Hackers who have been guests on Hack the Process with M. David Green. This week we’ve got holiday mentors, profitable podcasts, trauma therapy, and more. For all the links, check out the show notes at http://www.hacktheprocess.com/2018-12-04-process-hacker-news-holiday-mentors-profitable-podcasts-trauma-therapy-and-more/. Enjoy! Events Why not eat, drink, be merry, and get mentored by design experts this season? Cascade SF, founded by Andi Galpern, is bringing you Holiday Mentor Night on December 11 in San Francisco. If you missed out on Finish Up Weekend last September, here’s another chance to join! Alex Hillman invites you to get on the list while it’s early and enjoy a weekend of productivity from January 25 to 27 at Indy Hall in Philadelphia. Courses Become a certified coach through Courageous Living Coach Certification, a training program created by Kate Swoboda. Registration for 2019 trainees is open until December 10. Media The National Business Book Awards official podcast, Curating the Business Conversation, recently kicked off their first episode, which features Jennifer Riel discussing integrative thinking as described in her book, Creating Great Choices. Entrepreneur and podcaster Jordan Harbinger joins Tom Morkes on the In the Trenches Podcast to share some of his secrets about starting and growing a profitable podcast. Seth Godin was also a recent guest on Tom’s show. Jessica Spaulding of Harlem Chocolate Factory shares her ups and downs as an entrepreneur on the Side Hustle Pro podcast, hosted by Nicaila Matthews-Okome. Writing Have you heard that Tara Hunt is dropping the word “Social” from the Truly Social brand? Read her newest LinkedIn post to learn why. Christine Henderson just published this interview with Maria Dismondy, in which Maria shares her background, her writing journey, and her approach to social media management. Recommended Resources On The Trauma Therapist with Guy Macpherson, who was recommended by Lisa Dale Miller, Trauma Consultant Margi Bennett speaks about human rights and trauma advocacy. Kimberly Bryant, one of Michelle Kim’s influences, shares her background, the foundation, and the mission of Black Girls Code in an article on Success. The Healing Power of Mindfulness, the third installment of the Coming to Our Senses series by Jon Kabat-Zinn, is now out. Jon is a prominent figure in the mindfulness community who has touched the lives of many practitioners, such as Bill Duane, Rhonda Magee, and Lisa Dale Miller. Thanks for checking out this Process Hacker News update from Hack the Process. If you liked what you saw, please leave a comment to let us know what processes you’re hacking.

Broadband Conversations
Kimberly Bryant, Founder of Black Girls Code

Broadband Conversations

Play Episode Listen Later Oct 31, 2018 21:22


Kimberly Bryant, the founder of Black Girls Code, joined Commissioner Rosenworcel for a conversation about how we can encourage more girls, especially girls of color, to be interested in technology and how through that work we can help close the digital divide.

Hack the Process: Mindful Action on Your Plans
2018-10-02 Process Hacker News

Hack the Process: Mindful Action on Your Plans

Play Episode Listen Later Oct 2, 2018 4:42


Rentable Fashion, Courageous Parenting, Effective Moonlighting, and More Welcome to the Process Hacker News, your weekly roundup of useful news and updates from Process Hackers who have been guests on Hack the Process with M. David Green. This week we’ve got rentable fashion, courageous parenting, effective moonlighting, and more. For all the links, check out the show notes at http://www.hacktheprocess.com/2018-10-02-process-hacker-news-rentable-fashion-courageous-parenting-effective-moonlighting-and-more/ Enjoy! Launches Congratulations to Rent the Runway for opening up a standalone shop in San Francisco! The fashion startup, where Hampton Catlin is VP of Engineering, lets members rent special occasion clothing for a monthly subscription fee. Events Some seats are still available at Integrative Thinking for Leaders on October 5 in Toronto, where Jennifer Riel will teach participants to use differences to create new options. Media Teens can be tough to manage, but you can listen to some tips from Psychologist Dr. Ken Wilgus as he shares his knowledge about effective parenting of teens in the newest episode of the Groundless Ground Podcast by Lisa Dale Miller, As a guest on the Raiseology Podcast, Kate Swoboda discusses the courage habit and how it applies to the challenges of being a parent. On the Building A Business That Lasts Podcast, Gavin Zuchlinski talks about Acuity Scheduling’s history and growth, and how the appropriate work attitude can support learning and decision-making. Curtis McHale compares running a business to running a race, and sheds light on what happens when the going gets tough for a business. In Tiffani Bova’s Let’s Be Clear interview at Dreamforce 2018, she shares the wisdom learned from the thirty companies she talked to for her book, Growth IQ. Writing Got a full-time job? Ryan Waggoner recommends you try moonlighting as a freelancer and provides several great reasons why in his updated article. If you’ve been exercising and eating well, but these healthy habits are leaving you worn out, Julian Hayes II suggests eleven possible reasons in his recent article. As a Mindfulness Instructor, Andrew Nance shares some ways to use performing arts to help children express emotions instead of acting out. On Quora, Rich Mironov gives his thoughts on the challenges of product acquisition for a large tech company, as opposed to new product development. Recommended Resources Tony Robbins is a motivational speaker who was mentioned as an inspiration by Byron Morrison, Jay Wong, Adam Siddiq, and Luis Congdon, and on his recent podcast episode, he chats with author and leadership expert Sam Walker about what it means to be a true leader. Sixth, seventh, and eighth graders can register now for the Black Girls CODE Computer History Museum Enrichment Event on October 28 in Mountain View, California to learn about designing and coding. Kimberly Bryant, the founder of Black Girls Code, was recommended by Michelle Kim. Thanks for checking out this Process Hacker News update from Hack the Process. If you liked what you saw, please leave a comment to let us know what processes you’re hacking.

Black Tribbles
348 - A MisogyNO Conversation w Kimberly Bryant and Chauna Lawson

Black Tribbles

Play Episode Listen Later Sep 7, 2018 115:08


The Tribbles welcome Black Girls Code's KIMBERLY BRYANT and professional organizer/cosplayer/GreekGeek CHAUNA LAWSON to the ongoing conversation about sexism and chauvinism in geek spaces such as the tech world and convention floors. --- Send in a voice message: https://anchor.fm/black-tribbles/message

Hack the Process: Mindful Action on Your Plans
2018-07-10 - Process Hacker News from Hack the Process Podcast

Hack the Process: Mindful Action on Your Plans

Play Episode Listen Later Jul 10, 2018 4:31


Mindful Veterans, Scalable Sales, Training Wheels, and More Welcome to the Process Hacker News, your weekly roundup of useful news and updates from Process Hackers who have been guests on Hack the Process with M. David Green. This week we’ve got mindful veterans, scalable sales, training wheels, and more. For all the links, check out the show notes at http://www.hacktheprocess.com/process-hacker-news-for-july-10-2018-mindful-veterans-scalable-sales-training-wheels-and-more/ Enjoy! Events Get your giggle on with Sarah Cooper at the Laughing Buddha Comedy Showcase at the Triad Theater in New York on July 24. Woman veterans can now enroll in the upcoming Veteran’s Path Anchor Program which begins on July 15. In this four-month program in Colorado, vets can expect to learn and practice mindfulness tools that can support them in their daily lives. Bill Duane is the Vice Chairman of the Board at Veteran’s Path. Awards Congratulations to Jennifer Riel and Roger Martin for winning in the strategy and leadership category of Emerald Publishing’s Awards for Excellence 2018 with their article, An Integrative Methodology for Exploring Decision Choices. Launch RightMessage by Brennan Dunn is launching RightAsk, which is a great way to survey and segment your audience. Media If time is constantly an issue for you, listen to an interview with Curtis McHale as he shares some secrets for making the most of your limited time on The Productivity Show. Discover how to build an effective scalable sales process from this Close.io webinar featuring Steli Efti, Mike Sutherland, Mike Paladino, and Vinay Patankar. Get some tips on side hustling and stock trading from investor Tela Holcomb, who was interviewed by Nicaila Matthews-Okome for the Side Hustle Pro podcast. Nicole Holland guests on the Marketer of the Day Podcast with Robert Plank to discuss interviews that convert, targeting the proper audience, and building your network. Writing What do McDonald’s and Petco have in common? A sales-increasing marketing technique you can learn from! Luis Congdon writes about an effective marketing tactic that you can use for your business. Recommended Resources Akimbo, a podcast by Seth Godin has a new episode in which Seth talks about juggling, bicycles, and training wheels and how all these apply to life. Alex Cespedes and Tara Byrne are both fans of Seth Godin. Congratulations to Kimberly Bryant, one of Michelle Kim’s influences, for being honored at the annual McDonald’s 365Black Awards, which gives recognition to individuals who have had a positive impact on the African-American community. Tune in to Grow Your 1099, a podcast hosted by Josh Jones and Mitchell Levy, who was referenced by Kimberly Wiefling. Mitchell himself takes the guest chair in the latest episode, sharing details about his work-life balance. Thanks for checking out this Process Hacker News update from Hack the Process. If you liked what you saw, please leave a comment to let us know what processes you’re hacking.

Hack the Process: Mindful Action on Your Plans
2018-05-22 Process Hacker News from Hack the Process Podcast

Hack the Process: Mindful Action on Your Plans

Play Episode Listen Later May 22, 2018 3:56


Welcome to the Process Hacker News, your weekly roundup of useful news and updates from Process Hackers who have been guests on Hack the Process with M. David Green. This week we’ve got PR misconceptions, talent strategies, book reviews, and more. Enjoy! Media Increase your focus dramatically by asking yourself this question that Jay Wong has for you. Doing things differently may just help you find your way to success. Listen to how Josh Haynam adjusted his process and approach until his company became a hit on the Make It Snappy Show. Curtis McHale has been publishing book reviews on his podcast, Should I Read It, and his most recent review is of a book by Michael Hyatt titled Your Best Year Ever. Listen in! Pamela Wasley advises entrepreneurs to be strategic with talent on the Absolute Advantage podcast. Writing In honor of International Day Against Homophobia, Biphobia, Intersexism, and Transphobia, Michelle Kim has written a little reminder for the LGBTQI community. What are the three common customer misconceptions about public relations? You can find out from an article written by Ricky Yean. A personal story about how a friend cheated him for money is shared by Luis Congdon, and he reveals the lessons he learned from this unfortunate experience in his latest Entrepreneur article. Michelle Dale gets more personal in her recent blog post and tells about the true story of her path to confidence and self-discovery as a digital nomad. The founder of Burning Man, Larry Harvey, recently passed away, and Loïc Le Meur wrote a post in his honor. Recommended Resources One of the folks that Michelle Kim looks up to, Kimberly Bryant of Black Girls Code, has been honored at Silicon Valley Forum’s 21st Visionary Awards. Congratulations, Kimberly! Are you working your schedule or is your schedule working you? Find out on this episode of the Here and Now Toronto Podcast with guest Eric Termuende, a workplace expert recommendation made by Tara Byrne. Amy Hoy, referenced by Alex Hillman, has written a blog post ranking nine ways to make software-as-a-service (Saas) customers hate you. Thanks for checking out this Process Hacker News update from Hack the Process. If you liked what you saw, please leave a comment to let us know what processes you’re hacking.

Making Ways
One Year of Making Ways: 50 Creators, Artists, and Entrepreneurs on Navigating Your Creative Career Path

Making Ways

Play Episode Listen Later Apr 13, 2018 42:11


It’s time to celebrate one year of Making Ways podcast! 50 creative minds, 50 career journeys, 50 compelling conversations…50 episodes. Over the past year, host, marketer, and illustrator Rob Goodman has sat down with creatives of all stripes to discuss the winding journeys to their most fulfilling careers. Our guests have navigated unexpected paths and come out on the other side to share pitfalls, lessons learned, and amazing advice. These learnings can help any creative-minded professional pursue a career that will bring them joy—and have an oversized impact on their communities too. In today’s episode, we look back at some of the interviews that listeners have loved. Hear insightful stories from artists, innovators, and entrepreneurs like Kelly Inouye, Alexandra Kenin, Windy Chien, Paul Madonna, Susa Cortez, George McCalman, Steve Vassallo, and Kimberly Bryant. Thanks for an amazing first year of Making Ways. Cheers to more stories and inspiration ahead to help you chart a creative path all your own.

Blackademia
Ep 4: Black Women in Ed Are Lit!

Blackademia

Play Episode Listen Later Mar 28, 2018 33:39


In this episode Tiffany and Autumn shed light on Black Women's Literary Societies, highlight Kimberly Bryant (the real life Princess Shuri), and discuss the privilege and toxic masculinity of the people committing school shootings. Black Women's Literary Societies journals.psu.edu/phj/article/viewFile/25504/25273 Black Women and Girls in STEM blavity.com/we-need-to-talk-abo…utm_campaign=buffer www.blackgirlscode.com/about-bgc.html School Shootings www.theguardian.com/world/2018/feb/…how-many-so-far www.harpersbazaar.com/culture/politi…c-masculinity/

NYSAIS-Now
Interview with Kimberly Bryant

NYSAIS-Now

Play Episode Listen Later Mar 13, 2018 18:55


In this NYSAIS-Now interview at the NYSAIS Education & Information Technology conference in January of 2018, Kimberly Bryant discusses the success of her non-profit, Black Girls CODE, and how she grew it from an afterschool program for her daughter into an international organization that has served over 3,000 students. Ms. Bryant also offers advice to schools on child wellness, connecting with students across demographics to get them interested and keep them coming back to programs they would not normally seek out, as well as how partnerships have helped Black Girls CODE throughout their history.

Four Minutes with On The Dot
Episode 37: Kimberly Bryant: She’s Increasing Tech Diversity - On The Dot Woman

Four Minutes with On The Dot

Play Episode Listen Later Dec 14, 2017 4:07


Have you ever noticed the most ignorant opinions often come from people who are stuck in the antiquated days of the past? For instance, I’m thinking of guys who think women don’t belong in certain industries and spout off idiotic notions like, “Women shouldn’t go into space! Women can’t code!” I’m saying no more to sexist ideas, no more to outdated, foolish dudes and no more to women being held back from accomplishing whatever we want in life and work! The post Kimberly Bryant: She’s Increasing Tech Diversity appeared first on On The Dot Woman.

Making Ways
Kimberly Bryant, Founder and Executive Director of Black Girls Code

Making Ways

Play Episode Listen Later Aug 18, 2017 43:13


On this episode of Making Ways, the podcast all about the unexpected paths to a creative career, host Rob Goodman is joined by Kimberly Bryant, founder and executive director of Black Girls Code. Kimberly’s path to starting the organization was anything but a straight shot. After an illustrious career in engineering spent working for Genentech, Novartis Vaccines and Diagnostics, Merck, and Pfizer, among others, she was drawn to launch Black Girls Code after seeing her daughter face the same challenges Kimberly confronted as a young woman of color interested in STEM (science, technology, engineering, and mathematics). Knowing that she could have a hand in improving the technology sector for her daughter and young women everywhere, Kimberly went all in. Today, Kimberly and Black Girls Code are dedicated to empowering young African American women, ages 7–17, by introducing them to and teaching computer programming and technology. In a wide-ranging, uplifting conversation, Kimberly recounts the twists and turns of her journey, from her upbringing in Memphis to the imposed career move into IT that eventually ignited a passion for technology, discussing the difficult times she went through as one of the only African American women studying engineering in college and the mentorship that got her through those rough times. She also shares the lessons she learned as a female leader in a male-dominated field and why she risked it all—even cashing out her 401(k)—to start Black Girls Code, as well as her advice for anyone feeling marginalized in the classroom or the workplace on how to make it through and thrive. We talk about the roadblocks to getting more women and women of color into the engineering field and the reasons diversity is critical to the world of technology: a broader range of voices at the table creates better products that can improve the lives of everyone. Tune in to the discussion with the incredible Kimberly Bryant for inspiration, motivation, and lessons you can apply to your life and creative work today.

52 Reasons Podcast
Ep 1.18 Black Girls Code

52 Reasons Podcast

Play Episode Listen Later May 12, 2017 13:40


We had the privilege to chat with Tiana from Black Girls Code to learn more about the work they are doing in their 13 chapters in the United States (plus one in South Africa) in this week's episode of the 52 Reasons Podcast. Tiana and I chatted about the history of Black Girls Code and the work of their talented and action oriented founder, Kimberly Bryant. We also had a chance to talk about the future of women of color in STEM fields, the importance of getting young girls involved in STEM related activities and most importantly, we talked about how you can get involved. 52 Reasons is focused on creating positive change in our neighborhoods and across the country. To learn more about the topics in this episode and how you can get involved, visit 52reasons.org.

Crosscurrents
4/10: When coding became male

Crosscurrents

Play Episode Listen Later Apr 10, 2017 26:49


Planet Money tells the story of when and why women stopped coding.Kimberly Bryant opens tech up to black girls.A first-hand account of the Apollo 11 mission.

Lean Startup
Using Lean Startup To Do Plenty With Very Little | Kimberly Bryant

Lean Startup

Play Episode Listen Later Mar 23, 2015 13:20


Black Girls Code is a fast-growing non-profit that started with the kernel of an idea in Oakland, CA and has been iterating its way across the country. Kimberly will talk about Lean Startup ideas have helped the organization meet its vision.

OF10podcast
9/10 Kimberly Bryant of BlackGirlsCode & Semil Shah of Haystack Fund

OF10podcast

Play Episode Listen Later Feb 26, 2015 63:00


On this episode, I talk to the dynamic Kimberly Bryant of BlackGirlsCode. Kimberly was a professional biotechnology engineer before founding the program and has been recognized by Oprah Winfrey and Toyota for her work during Oprah's The Life You Want tour. She's been commended by the White House as a tech champion, and has been profiled by Forbes, MademeNoire, Marie Claire, and CNN. Part 2 features a discussion with Semil Shah, a VC at Haystack Fund on his ideas on minority tech entrepreneurship, and his thoughts on how we might find solutions to issues in investment capital.

Your15Minutes Radio Network
In The Spotlight with Jaimi Alexander with Special Guest Kimberly Bryant

Your15Minutes Radio Network

Play Episode Listen Later Nov 9, 2014 30:00


Join Jaimi as she speaks with Black Girls Code Founder Kimberly Bryant during our celebration of the Texas Conference For Women Week.  Follow the conversation @queenjaimi and @your15minradio.

Revision Path
043: Kimberly Bryant

Revision Path

Play Episode Listen Later Sep 8, 2014 45:10


Kimberly Bryant is a force of nature. As the founder and executive director of Black Girls Code, she has been a tireless advocate for increasing the number of women in STEM fields through mentorship, workshops, and networking opportunities for young and pre-teen girls of color. The mission: introduce programming and technology to a new generation of coders who will become builders of technological innovation and of their own futures. But I wanted to go a bit beneath the surface and learn more about Kimberly Bryant herself. We did talk a lot about diversity in the tech fields and how Black Girls Code also embraces the STEAM movement, but we also talked about her own path to success (and where she wants to go in the future). It's a really great interview with someone who is a real game-changer in this industry. Enjoy! Black Girls Code Black Girls Code on Twitter Black Girls Code on Facebook

Spectrum
Tanya Woyke and Chris Rinke

Spectrum

Play Episode Listen Later Oct 4, 2013 30:00


TranscriptSpeaker 1: Spectrum's. Next. Speaker 2: N. N. N. N. Speaker 3: [inaudible].Speaker 1: Welcome to spectrum the science and technology show on k a l x, [00:00:30] Berkeley, a biweekly 30 minute program, bringing you interviews, featuring bay area scientists and technologists as well as a calendar of local events and news. Speaker 4: Good afternoon. I'm Rick Karnofsky. Brad swift and I are the hosts of today's show. Today we're talking with doctors, Tonya Wilkie and Chris Rink of the Department of Energy Joint Genome Institute in Walnut Creek. They recently published an article entitled insights into the Phylogeny and coding potential [00:01:00] of microbial dark matter in which they have to characterized through relationships between 201 different genomes and identified some unique genomic features. Tonya and Chris, welcome to spectrum. Speaker 5: Thanks for having us. Thank you. Speaker 4: So Tanya, what is microbial dark matter? Speaker 5: We like to take life as we know it and put it in an evolutionary tree in a tree of life. And what this assists us is to figure out the evolutionary histories of organisms and the relationships between [00:01:30] related groups of organisms. So what does this mean? It's to say we take microbial diversity as we know it on this planet and we place it in this tree of life. What you will find is that there will be some major branches in this tree, about 30 of them, and we call these major branches Fila that are made up of organisms that you can cultivate. So we can grow them on plates in the laboratory, we can grow them in Allen Meyer, flask and liquid media. We can study that for CLG. We can figure out what substrates they metabolize, [00:02:00] we can figure out how they behave under different conditions. Speaker 5: Many of them we can even genetically modify. So we really know a lot about these organisms and we can really figure out, you know, how do they function, what are the genetic underpinnings that make them function the way they do in the laboratory and also in the environment where they come from. So now coming back to this tree of life, if you keep looking at this tree of life, uh, we will find at least another 30 off these major branches that we refer to as [00:02:30] Canada. Dot. Sila and these branches have no cultivators, representatives, so all the organisms that make up these branches, we have not yet been able to cultivate in the laboratory. We call these kind of dot, Fila or microbial dark matter. And the term dark matter. All biological dark matter has been coined by the Steve Craig Laboratory at Stanford University when they published the first genomes after a candidate, phylum TM seven. We know that dark matter is in most if not all [00:03:00] ecosystems. So we find it in most ecosystems, but to get at their complete genetic makeup. That's the key challenge. Speaker 4: Yeah. And if you, if you want to push it through the extreme, there are studies out there estimating the number of bacteria species they are and how many we can cultivate. And the result is all there. The estimation of the studies we can cultivate about, you know, one or 2% of all the microbial species out there. So basically nine to 9% is still out there and we haven't even looked at it. So this really, this major on culture microbes and majority is [00:03:30] still waiting out there to be explored. So that sort of carries on the analogy to cosmological dark matter in which there's much more of it than what we actually see and understand. Right. Speaker 5: So how common and how prevalent are, are these dark matter organisms? Yeah, that's a really good question. So in some environments they are what we would consider the rabbi biosphere. So they are actually at fairly low abundance, but our methods are sensitive enough to still pick them up. [00:04:00] In other environments. We had some sediment samples where some of these candidate file, our, actually what we would consider quite abandoned, it's a few percent, let's say 2% of opiate candidate phylum that to us, even 2% is quite abandoned. Again, you have to consider the whole community. And if one member is a 2%, that's, that's a pretty dominant community members. So I'd arise from environment, environment Speaker 4: and Chris, where were samples collected from? So altogether we sampled nine sampling sites all over the globe [00:04:30] and we tried to be as inclusive as possible. So we had marine samples, freshwater samples, sediment samples, um, some samples from habitats with very high temperatures and also a sample from a bioreactor. And there were a few samples among them that for which we had really great hopes. And among them were um, samples from the hot vans from the bottom of Pacific Ocean. The samples we got were from the East Pacific virus sampling side, and that's about 2,500 meters below the store phase. And [00:05:00] the sample there, you really need a submersible that's a small submarine and you can launch from a research vessel. In our case, those samples were taken by Elvin from the woods hole oceanographic institution and now you have a lot of full Canik activity and also the seawater seeps into the earth crust goes pretty deep and gets heated up. Speaker 4: And when it comes back out as a hydrothermal event, it has up to [inaudible] hundred 50 to 400 degrees Celsius. And it is enriched in chemicals such as a sulfur or iron. [00:05:30] It makes us immediately with the surrounding seawater, which is only about a two degrees Celsius. So it's a very, it's a very challenging environment because you have this gradient from two degrees to like 400 degrees within a few centimeters and you have those chemicals that uh, the organisms, the micro organisms could use blast. There is no sunlight. So we thought that's a very interesting habitat to look for. Microbial, dark matter. There were several samples. That's a to us. One of them is the Homestake [00:06:00] mine in South Dakota and that's an old gold mine that is not used anymore since 2002 but are there still scientific experiments going on there? It's a very deep mine, about 8,000 feet deep and we could all sample from about 300 feet. Speaker 4: And we were surprised about this Ikea diversity we found in those samples. There were a few Akia that were not close to any, I don't know another key out there for some of them. We even had to propose new archaeal Fila. Stepping back a bit, Chris, [00:06:30] can you tell us more about Ikea and perhaps the three domains of life? The three domains were really established by Culver's with his landmark paper in 1977 and what he proposed was a new group of Derek here. So then he had all together three domains. You had the bacteria and archaea and the eukaryotes, the eukaryote state. There are different one big differences to have the nucleus, right? They have to DNA in the nucleus and it also includes all the higher taxa. But then you have also their key and the bacteria. [00:07:00] And those are two groups that only single cell organisms, but they are very distant related to each other, the cell envelope, all. And also the cell duplication machinery of the archaea is closer to the eukaryotes than it is to the bacteria. Speaker 5: Yeah, and it's interesting, I mean Ikea, I guess we haven't sequenced some that much yet, but Ikea are very important too, but people are not aware of them. They know about bacteria, but Ikea and maybe because there aren't any RKO pathogen [00:07:30] and we'd like to think about bacteria with regards to human health, it's very important. That's why most of what we sequence are actually pathogens, human pathogens. So we sequence, I don't know how many strains of your senior pastors and other pathogenic bacteria, but archaea are equally important, at least in the environment. But because we rarely find them associated with humans, we don't really think about archaea much. Our people aren't really aware of Ikea. Speaker 4: Talk about their importance, Speaker 5: the importance [00:08:00] in the environment. So Ikea are, for example, found in extreme environments. We find them in Hydro Soma environments. We find them in hot springs. Uh, we, they have, they have biotechnological importance and not a lot of, quite useful in enzymes that are being used in biotechnology are derived from Ikea in part because we find them in these extreme environments and hot environments and they have the machinery to deal with this temperature. So they have enzymes that function [00:08:30] properly at high temperature and extreme conditions, really extreme on the commerce extreme or fields. And that makes them very attractive bio technologically because some of these enzymes that we would like to use should be still more tolerant or should have these features that are sort of more extreme. Um, so we can explain it them for a biotech technological applications. [inaudible] Speaker 6: [inaudible] [00:09:00] you are listening to spectrum on k l x Berkeley. I'm Rick [inaudible] and I'm talking with Kanya vulgate and Chris, her and Kate about using single cell genomics. You're expand our knowledge that the tree of life, Speaker 5: [00:09:30] so again, we called up a range of different collaborators and they were all willing to go back to these interesting sites, even to the hydrothermal vent and get us fresh sample. No one turned us down. So we, we, we screened them again to make sure they are really of the nature that we would like to have them and the ones that were suitable. We then fed into our single cell workflow. Can you talk briefly about that screening? There were two screens in waft. One screen was narrowing down the samples themselves and we received a lot more sample, I would say at least [00:10:00] three times as many sample as we ended up using. And we pre-screened these on a sort of barcode sequencing level. And so we down selected them to about a third. And then within this third we sorted about 9,000 single cells and within these 9,000 single cells, only a subset of them went through successful single cell, whole genome amplification. And out of that set then we were only, we were able to identify another subset. And [00:10:30] in the end we selected 200 for sequencing 201 Speaker 4: and how does single cell sequencing work? Speaker 5: So to give you a high level overview, you take a single cell directly from the environment, you isolate it, and there's different methodologies to do that. And then you break it open, you expose the genetic material within the cell, the genome, and then you amplify the genome. And some single cells will only have one copy of that genome. And we have a methodology, it's a whole genome amplification process that's called multiple displacement amplification [00:11:00] or MDA. And that allows us to make from one copy of the genome, millions and billions of copies. One copy of the genome corresponds to a few family or grams of DNA. We can do much with it. So we have to multiply, we have to make these millions and billions of copies of the genome to have sufficient DNA for next generation sequencing. Speaker 4: Are there other extreme environments that you guys didn't take advantage of in this study that might be promising? Definitely. Um, so we, [00:11:30] we created the list already off environments that would be interesting to us based on, you know, on the results from the last start in the experience we have with environmental conditions and the is microbes we've got out of it. So we're definitely planning to have a followup study where we explore all those, um, habitats that we couldn't include in this, uh, study. Speaker 5: So some examples of the Red Sea and some fjords in Norway and their various that were after Speaker 4: the, that the Black Sea is a very interesting environment too. It's, it's completely anoxic, high levels of sulfide [00:12:00] and it's, it's really, it's huge. So that's a very interesting place to sample too. And how historically have we come to this tree in the old days? And I mean the, the, the pre sequencing area, um, the main criteria that scientists use to categorize organisms whilst the phenotype. That's the, the morphology, the biochemical properties, the development. And that was used to put, uh, organisms into categories. And then with the dawn of the sequencing area, and that was [00:12:30] mainly, um, pushed by the Sanger sequencing, the development of the Sanger sequencing in the 70s. We finally had another and we could use and that was the DNA sequence of organisms. And that was used to classify and categorize organisms. Does a phenotyping still play a role in modern phylogeny? It still does play a role in modern philosophy in the, especially for eukaryotes. Speaker 4: Well you have a very significant phenotype. So what you do there is you can compare a phenotyping information with the [00:13:00] genomic information and on top of that even, uh, information from all the ontology and you try to combine all the information you have doing for, let's say, for the evolutionary relationships among those organisms in modern times, the phylogeny of bacteria, Nokia, it's mainly based on molecular data. Part of our results were used to infer phylogenetic relationships into the started. The evolutionary history of those microbes. We'll be, well do you have for the first time is we now have chine [00:13:30] ohms for a lot of those branches of the tree where before we only had some barcodes so we knew they were there, but we had no information about the genomic content and they'll seem to be hafted for the first time. We can actually look at the evolutionary history of those microbes and there were two, two main findings in our paper. Speaker 4: One was that for a few groups, the f the placement that taxonomic placement in the tree of life was kind of debated in the past. We could help to clarify that. For example, one group is they clock chemo needs [00:14:00] and it was previously published. It could be part of the farm of the spiral kids, but we could Cully show with our analysis that they are their own major branch entry of laughter or their own file them and a a second result. That's, I think it's very important that that's because they didn't share a lot of jeans with others. Bifurcates is that, that's, that's right. So if you placed him in a tree of life, you can see that the don't cluster close parakeets, they'll come out on the other side by out by themselves, not much resembling if the spark is there. And the second result was [00:14:30] that, uh, we found several of those main branches of the tree of life, those Fila the class of together consistently in our analysis. Speaker 4: And so we could group them together and assign super filer to them. One example is a sweet book, Zero Fila Debra Opa 11 or the one and Chino too, and also almost clustered together. So we proposed a super final name. Potesky and Potesky means I'm bear or simple. And we choose that because they have a reduced and streamlined genome. That's another common feature. [00:15:00] I'm Andrea and I, I have to say that, you know, looking into evolutionary relationships, it is, it is a moving target because as Tanya mentioned, especially for microbes and bacteria and like here, there's still so many, um, candidates that are out there for which we have no genomic information. So we definitely need way more sequences, um, to get a better idea of the evolutionary relationships of all the books. Your Nokia out there Speaker 6: [00:15:30] spectrum is a public affairs show about science on k a l x Berkeley. Our guests today are Tanya. Okay. And Chris Rink k you single cell genomics to find the relationships between hundreds of dark matter of microbes. Speaker 4: And can you speak to the current throughput? I would have thought that gathering up organisms in such extreme environments was really the time limiting factor. [00:16:00] But I suppose if you have this archive, other steps might end up taking a while. I will say the most time consuming step is really to to sort those single cells and then to lyse the single cells and amplify the genome and then of course to screen them for the, for genomes of interest for microbial like metagenomes [inaudible] that was a big part of the study. So actually getting the genomic information out of the single cells and if that can be even more streamlined than uh, and push to a higher or even more stupid level, I think [00:16:30] that will speed up the recovery of, of novel microbial dogmatic genomes quite a bit. Speaker 5: Well, we have a pretty sophisticated pipeline now at the JGI where we can do this at a fairly high throughput, but as Chris said, it still takes time and every sample is different. Every sample behaves different depending on what the properties of the samples are. You may have to be treated in a certain way to make it most successful for this application and other staff in the whole process that takes a long time is the key. The quality control [00:17:00] of the data. So the data is not as pretty as a sequencing data from an isolet genome where you get a perfect genome back and the sequence data that you get back is fairly, even the coverage covered all around the genome. Single cell data is messy. The amplification process introduces these artifacts and issues. It can introduce some error because you're making copies of a genome. Speaker 5: So errors can happen. You can also introduce what we call comeric rearrangement. That means that pieces of DNA [00:17:30] go together that shouldn't go together. Again, that happens during the amplification process. It's just the nature of the process. And on top of that, parts of the genome amplify nicely and other parts not so nice. So the overall sort of what we call sequence coverage is very uneven. So the data is difficult to deal with. We have specific assembly pipelines that we do. We do a sort of a digital normalization of the data before we even deal with the data, so it's not as nice. And then on top of that you can have contamination. So the whole process is very [00:18:00] prone to contamination. Imagine you only have one copy of a single cell, five Phantogram, one circle of DNA and any little piece of DNA that you have in that prep that sometimes as we know comes with the reagents. Speaker 5: Because reagents are not designed to deal with such low template molecules. They will call amplify, they will out-compete or compete with your template. So what you end up with in your sequence is your target and other stuff that was in was in the reagents or again, in your prep. We have very rigorous [00:18:30] process of cleaning everything. We you read a lot of things we sterilize, so we need to get rid of any DNA to not, um, to, to have a good quality genome in the end. And so that said, we have developed tools and pipelines at our institute now that specifically help us detect contamination. Sometimes it's not easy to detect it and then remove it. We want to make sure that the single cell genomes that we released at as single cell genome ABC are really ABC and not a plus x and [00:19:00] B plus k because accidentally something came along and contaminated the prep. And especially with candidate Fila, it's, it's fairly difficult to detect tech contamination because what would help us would be if we would have referenced genomes, we're actually generating this reference genome so we don't have a good reference to say, yeah, this is actually, that's our target organism and the rest is public contamination, so it's very tricky. Speaker 4: Are there other examples for [00:19:30] single cell sequencing being used on this many organisms Speaker 5: on this many organisms? No, not that I'm aware of. I know there's an effort underway and the h and p, the human microbiome project where they also identified there, they nicely call it the most wanted list, so they have the target organisms that are quite abundant in different microbiomes within the human body associated with the human body and they've been very successfully able to cultivate. A lot of them bring a lot of them in culture [00:20:00] and it may be easier for the h and p because we can mimic the conditions within the body a little bit better and more controlled. We know our body temperature and we know sort of what the middle year is in the different parts of our body. So it's a little bit easier to bring these organisms and culture than going to the hydrothermal vent and try and recreate these conditions which are extremely difficult to recreate. So that said, um, there are some that they are now targeting with single cell sequencing. So that's another large effort [00:20:30] that I know of that's specifically using single cell genomics to get at some of these reference genomes. Speaker 4: Can you get more out of this then? Sort of phylogenetic links? We found a few unique genomic features and one on one dimension is we found a recode. It's stopped caught on in, in two of those, a bacteria from the hot vans I mentioned earlier. And to give you a little bit of background, so, um, it's, we know the genetic information of each sale is and coded in its DNA, but in order to [00:21:00] make use of this genomic information, this genetic information has to be translated into proteins. And then proteins that could be enzymes that are employed in the metabolism to keep the cell going. And a dispensation is pretty universal between the three domains of life. The way it works, we have three basis in your DNA and three basis are called the core done. And each call is translated in the one amino acid. Speaker 4: So this way you'll build a chain of amino acids and then this chain is for a folder [00:21:30] and then you have your ready made protein. This call them triplet. This three basis also work for start and stop. So there are certain colons that tell the cell, okay, that's where you start a protein. And another called in to tell us the cell. So that's, that's where you enter prod and you're done with it. There are some slight variations, but in general does a universally called, is perceived between all three domains of life. And what we found was very interesting in two of those bacteria from the hot vans. Ah, those two caecilian bacteria, we found the [00:22:00] recording. So one of the accord on did not called for a stop code on anymore, but in the quarter's for an amino acid in that case, glycine. And that has never been seen before. Were you surprised by these results? Speaker 5: To us, they were surprising because they were unique and they were different. On the other hand, I have to say I'm not that surprised because we haven't, like Russ said, we haven't looked at heart yet and considering that we can only cultivate a few percent of all the microbial diversity that exists on this planet as far as, [00:22:30] as far as we know it, it's not that surprising that you find these novel functions and there's these unique features and novel genetic codes because it's really, it's a highly under-explored area. Speaker 4: It is very rewarding. But if you look in the future, um, how much is still out of the sequence? Of course we're interested in that. So we looked at all the files show diversity that's known, that's out there based on this, um, biomarkers that Tony mentioned earlier and we just compared it to the genomes that we have sequenced so far. And we really want [00:23:00] to know, so if you want to cover let's say about 50% of all the fall diversity that's out there, how many achievements do we still have to sequence and the number of the estimate was we need to sequence at least 16,004 more genomes Speaker 5: and this is a moving target. So this is as we know, diversity of today it and every day we sample my environments, we sequence them deeper and everyday our diversity estimates increase. So what we've done with these 201 it's the tip of the iceberg but it's a start. Speaker 4: [00:23:30] Well Tanya and Chris, thanks for joining us. Thanks for having us. Thanks for having us. Yeah. Speaker 6: [inaudible] that's what shows are archived on iTunes to you. We've queued a simple link for you. The link is tiny, url.com/calex Speaker 7: spectrum Speaker 8: irregular feature of spectrum is a calendar [00:24:00] of some of the science and technology related events happening in the bay area over the next two weeks. Here's Brad swift and Renee Rao here today. Majority tomorrow. Expanding technological inclusion, technological inclusion is not an issue for some of us. It is an issue for all of us. Mitchell Kapore, co-chair of [inaudible] center for social impact and a partner at Kapore capital. We'll moderate a panel discussion among the following [00:24:30] presenters, Jennifer r Guayle, executive director of Latino to Kimberly Bryant, founder of Black Girls Code Connie Mack Keebler, a venture capitalist with the collaborative fund. Vivek Wadhwa academic researcher, writer and entrepreneur here today. Majority tomorrow is free and open to everyone on a first come first seated basis. This is happening on the UC Berkeley campus in Soutar de Di Hall [inaudible] [00:25:00] Auditorium Monday October 7th at 4:00 PM Speaker 7: the second installment of the six part public lecture series, not on the test. The pleasure and uses of mathematics will be held this October 9th Dr. Keith Devlin will deliver a lecture on underlying mathematics in video games. Dr Devlin will show how casual video games that provide representation of mathematics enabled children and adults to learn basic mathematics by playing in the same way people [00:25:30] learn music by learning to play the piano. Professor Devlin is a mathematician at Stanford, a Co founder and president of Inner Tube Games and the math guy of NPR. The lecture will be held on October 9th at 7:00 PM in the Berkeley City College Auditorium located at 2050 Center street in Berkeley. The event is free and open to the public. Speaker 8: The Leonardo arts science evening rendezvous or laser is a lecture series with rotating barrier venues. October 9th there will be a laser [00:26:00] at UC Berkeley. Presenters include Zan Gill, a former NASA scientists, Jennifer Parker of UC Santa Cruz, Cheryl Leonard, a composer, Wayne Vitali, founding member of gamelons Sakara [inaudible]. This is Wednesday, October 9th from 6:30 PM to 9:00 PM on the UC Berkeley campus in barrels hall room 100 Speaker 7: how can we prevent information technology [00:26:30] from destroying the middle class? Jaron Lanier, is it computer scientists, Kim Poser, visual artist and author. October 14th linear will present his ideas on the impact of information technology on his two most recent books are title. You are not a gadget and who owns the future. The seminar will be held in Sue Taja, Dai Hall, but not auditorium on the UC Berkeley campus. Monday, October 14th from 11:00 AM to noon [00:27:00] and that with some science news headlines. Here's the Renee, the intergovernmental panel on climate change released part of its assessment report. Five last Friday. The more than 200 lead authors on their report included Lawrence Berkeley National Labs, Michael Warner and William Collins who had a chapters on longterm climate change productions and climate models. The report reinforces previous conclusions that over the next century, the continents will warm [00:27:30] with more hot extremes and fewer cold extremes. Precipitation patterns around the world will also continue changing. One-Arm Collins noted that climate models since the last report in 2007 have improved significantly as both data collection and mechanistic knowledge have grown using these models. Scientists made several projections of different scenarios for the best, worst and middling cases of continued greenhouse emissions. Speaker 7: [00:28:00] Two recent accomplishments by commercial space programs are notable. Orbital Sciences launched their sickness spacecraft on September 18th a top the company's rocket and Tara's from wallops island, Virginia. On September 28th the Cygnus dock did the international space station for the first time, a space x rocket carrying and Canadian satellite has launched from the California coast in a demonstration flight of a new Falcon rocket. The next generation. Rocket boasts [00:28:30] upgraded engines designed to improve performance and carry heavier payloads. The rocket is carrying a satellite dead kiss IOP, a project of the Canadian Space Agency and other partners. Once in orbit it will track space weather. Speaker 2: Mm mm mm. Mm Huh. Speaker 7: The music [00:29:00] heard during the show was written and produced by Alex Simon. Yeah. Speaker 3: Thank you for listening to spectrum. If you have comments about the show, please send them to us via email. Address is [inaudible] dot [inaudible] dot com Speaker 9: [inaudible]. Hosted on Acast. See acast.com/privacy for more information.

Spectrum
Tanya Woyke and Chris Rinke

Spectrum

Play Episode Listen Later Oct 4, 2013 30:00


TranscriptSpeaker 1: Spectrum's. Next. Speaker 2: N. N. N. N. Speaker 3: [inaudible].Speaker 1: Welcome to spectrum the science and technology show on k a l x, [00:00:30] Berkeley, a biweekly 30 minute program, bringing you interviews, featuring bay area scientists and technologists as well as a calendar of local events and news. Speaker 4: Good afternoon. I'm Rick Karnofsky. Brad swift and I are the hosts of today's show. Today we're talking with doctors, Tonya Wilkie and Chris Rink of the Department of Energy Joint Genome Institute in Walnut Creek. They recently published an article entitled insights into the Phylogeny and coding potential [00:01:00] of microbial dark matter in which they have to characterized through relationships between 201 different genomes and identified some unique genomic features. Tonya and Chris, welcome to spectrum. Speaker 5: Thanks for having us. Thank you. Speaker 4: So Tanya, what is microbial dark matter? Speaker 5: We like to take life as we know it and put it in an evolutionary tree in a tree of life. And what this assists us is to figure out the evolutionary histories of organisms and the relationships between [00:01:30] related groups of organisms. So what does this mean? It's to say we take microbial diversity as we know it on this planet and we place it in this tree of life. What you will find is that there will be some major branches in this tree, about 30 of them, and we call these major branches Fila that are made up of organisms that you can cultivate. So we can grow them on plates in the laboratory, we can grow them in Allen Meyer, flask and liquid media. We can study that for CLG. We can figure out what substrates they metabolize, [00:02:00] we can figure out how they behave under different conditions. Speaker 5: Many of them we can even genetically modify. So we really know a lot about these organisms and we can really figure out, you know, how do they function, what are the genetic underpinnings that make them function the way they do in the laboratory and also in the environment where they come from. So now coming back to this tree of life, if you keep looking at this tree of life, uh, we will find at least another 30 off these major branches that we refer to as [00:02:30] Canada. Dot. Sila and these branches have no cultivators, representatives, so all the organisms that make up these branches, we have not yet been able to cultivate in the laboratory. We call these kind of dot, Fila or microbial dark matter. And the term dark matter. All biological dark matter has been coined by the Steve Craig Laboratory at Stanford University when they published the first genomes after a candidate, phylum TM seven. We know that dark matter is in most if not all [00:03:00] ecosystems. So we find it in most ecosystems, but to get at their complete genetic makeup. That's the key challenge. Speaker 4: Yeah. And if you, if you want to push it through the extreme, there are studies out there estimating the number of bacteria species they are and how many we can cultivate. And the result is all there. The estimation of the studies we can cultivate about, you know, one or 2% of all the microbial species out there. So basically nine to 9% is still out there and we haven't even looked at it. So this really, this major on culture microbes and majority is [00:03:30] still waiting out there to be explored. So that sort of carries on the analogy to cosmological dark matter in which there's much more of it than what we actually see and understand. Right. Speaker 5: So how common and how prevalent are, are these dark matter organisms? Yeah, that's a really good question. So in some environments they are what we would consider the rabbi biosphere. So they are actually at fairly low abundance, but our methods are sensitive enough to still pick them up. [00:04:00] In other environments. We had some sediment samples where some of these candidate file, our, actually what we would consider quite abandoned, it's a few percent, let's say 2% of opiate candidate phylum that to us, even 2% is quite abandoned. Again, you have to consider the whole community. And if one member is a 2%, that's, that's a pretty dominant community members. So I'd arise from environment, environment Speaker 4: and Chris, where were samples collected from? So altogether we sampled nine sampling sites all over the globe [00:04:30] and we tried to be as inclusive as possible. So we had marine samples, freshwater samples, sediment samples, um, some samples from habitats with very high temperatures and also a sample from a bioreactor. And there were a few samples among them that for which we had really great hopes. And among them were um, samples from the hot vans from the bottom of Pacific Ocean. The samples we got were from the East Pacific virus sampling side, and that's about 2,500 meters below the store phase. And [00:05:00] the sample there, you really need a submersible that's a small submarine and you can launch from a research vessel. In our case, those samples were taken by Elvin from the woods hole oceanographic institution and now you have a lot of full Canik activity and also the seawater seeps into the earth crust goes pretty deep and gets heated up. Speaker 4: And when it comes back out as a hydrothermal event, it has up to [inaudible] hundred 50 to 400 degrees Celsius. And it is enriched in chemicals such as a sulfur or iron. [00:05:30] It makes us immediately with the surrounding seawater, which is only about a two degrees Celsius. So it's a very, it's a very challenging environment because you have this gradient from two degrees to like 400 degrees within a few centimeters and you have those chemicals that uh, the organisms, the micro organisms could use blast. There is no sunlight. So we thought that's a very interesting habitat to look for. Microbial, dark matter. There were several samples. That's a to us. One of them is the Homestake [00:06:00] mine in South Dakota and that's an old gold mine that is not used anymore since 2002 but are there still scientific experiments going on there? It's a very deep mine, about 8,000 feet deep and we could all sample from about 300 feet. Speaker 4: And we were surprised about this Ikea diversity we found in those samples. There were a few Akia that were not close to any, I don't know another key out there for some of them. We even had to propose new archaeal Fila. Stepping back a bit, Chris, [00:06:30] can you tell us more about Ikea and perhaps the three domains of life? The three domains were really established by Culver's with his landmark paper in 1977 and what he proposed was a new group of Derek here. So then he had all together three domains. You had the bacteria and archaea and the eukaryotes, the eukaryote state. There are different one big differences to have the nucleus, right? They have to DNA in the nucleus and it also includes all the higher taxa. But then you have also their key and the bacteria. [00:07:00] And those are two groups that only single cell organisms, but they are very distant related to each other, the cell envelope, all. And also the cell duplication machinery of the archaea is closer to the eukaryotes than it is to the bacteria. Speaker 5: Yeah, and it's interesting, I mean Ikea, I guess we haven't sequenced some that much yet, but Ikea are very important too, but people are not aware of them. They know about bacteria, but Ikea and maybe because there aren't any RKO pathogen [00:07:30] and we'd like to think about bacteria with regards to human health, it's very important. That's why most of what we sequence are actually pathogens, human pathogens. So we sequence, I don't know how many strains of your senior pastors and other pathogenic bacteria, but archaea are equally important, at least in the environment. But because we rarely find them associated with humans, we don't really think about archaea much. Our people aren't really aware of Ikea. Speaker 4: Talk about their importance, Speaker 5: the importance [00:08:00] in the environment. So Ikea are, for example, found in extreme environments. We find them in Hydro Soma environments. We find them in hot springs. Uh, we, they have, they have biotechnological importance and not a lot of, quite useful in enzymes that are being used in biotechnology are derived from Ikea in part because we find them in these extreme environments and hot environments and they have the machinery to deal with this temperature. So they have enzymes that function [00:08:30] properly at high temperature and extreme conditions, really extreme on the commerce extreme or fields. And that makes them very attractive bio technologically because some of these enzymes that we would like to use should be still more tolerant or should have these features that are sort of more extreme. Um, so we can explain it them for a biotech technological applications. [inaudible] Speaker 6: [inaudible] [00:09:00] you are listening to spectrum on k l x Berkeley. I'm Rick [inaudible] and I'm talking with Kanya vulgate and Chris, her and Kate about using single cell genomics. You're expand our knowledge that the tree of life, Speaker 5: [00:09:30] so again, we called up a range of different collaborators and they were all willing to go back to these interesting sites, even to the hydrothermal vent and get us fresh sample. No one turned us down. So we, we, we screened them again to make sure they are really of the nature that we would like to have them and the ones that were suitable. We then fed into our single cell workflow. Can you talk briefly about that screening? There were two screens in waft. One screen was narrowing down the samples themselves and we received a lot more sample, I would say at least [00:10:00] three times as many sample as we ended up using. And we pre-screened these on a sort of barcode sequencing level. And so we down selected them to about a third. And then within this third we sorted about 9,000 single cells and within these 9,000 single cells, only a subset of them went through successful single cell, whole genome amplification. And out of that set then we were only, we were able to identify another subset. And [00:10:30] in the end we selected 200 for sequencing 201 Speaker 4: and how does single cell sequencing work? Speaker 5: So to give you a high level overview, you take a single cell directly from the environment, you isolate it, and there's different methodologies to do that. And then you break it open, you expose the genetic material within the cell, the genome, and then you amplify the genome. And some single cells will only have one copy of that genome. And we have a methodology, it's a whole genome amplification process that's called multiple displacement amplification [00:11:00] or MDA. And that allows us to make from one copy of the genome, millions and billions of copies. One copy of the genome corresponds to a few family or grams of DNA. We can do much with it. So we have to multiply, we have to make these millions and billions of copies of the genome to have sufficient DNA for next generation sequencing. Speaker 4: Are there other extreme environments that you guys didn't take advantage of in this study that might be promising? Definitely. Um, so we, [00:11:30] we created the list already off environments that would be interesting to us based on, you know, on the results from the last start in the experience we have with environmental conditions and the is microbes we've got out of it. So we're definitely planning to have a followup study where we explore all those, um, habitats that we couldn't include in this, uh, study. Speaker 5: So some examples of the Red Sea and some fjords in Norway and their various that were after Speaker 4: the, that the Black Sea is a very interesting environment too. It's, it's completely anoxic, high levels of sulfide [00:12:00] and it's, it's really, it's huge. So that's a very interesting place to sample too. And how historically have we come to this tree in the old days? And I mean the, the, the pre sequencing area, um, the main criteria that scientists use to categorize organisms whilst the phenotype. That's the, the morphology, the biochemical properties, the development. And that was used to put, uh, organisms into categories. And then with the dawn of the sequencing area, and that was [00:12:30] mainly, um, pushed by the Sanger sequencing, the development of the Sanger sequencing in the 70s. We finally had another and we could use and that was the DNA sequence of organisms. And that was used to classify and categorize organisms. Does a phenotyping still play a role in modern phylogeny? It still does play a role in modern philosophy in the, especially for eukaryotes. Speaker 4: Well you have a very significant phenotype. So what you do there is you can compare a phenotyping information with the [00:13:00] genomic information and on top of that even, uh, information from all the ontology and you try to combine all the information you have doing for, let's say, for the evolutionary relationships among those organisms in modern times, the phylogeny of bacteria, Nokia, it's mainly based on molecular data. Part of our results were used to infer phylogenetic relationships into the started. The evolutionary history of those microbes. We'll be, well do you have for the first time is we now have chine [00:13:30] ohms for a lot of those branches of the tree where before we only had some barcodes so we knew they were there, but we had no information about the genomic content and they'll seem to be hafted for the first time. We can actually look at the evolutionary history of those microbes and there were two, two main findings in our paper. Speaker 4: One was that for a few groups, the f the placement that taxonomic placement in the tree of life was kind of debated in the past. We could help to clarify that. For example, one group is they clock chemo needs [00:14:00] and it was previously published. It could be part of the farm of the spiral kids, but we could Cully show with our analysis that they are their own major branch entry of laughter or their own file them and a a second result. That's, I think it's very important that that's because they didn't share a lot of jeans with others. Bifurcates is that, that's, that's right. So if you placed him in a tree of life, you can see that the don't cluster close parakeets, they'll come out on the other side by out by themselves, not much resembling if the spark is there. And the second result was [00:14:30] that, uh, we found several of those main branches of the tree of life, those Fila the class of together consistently in our analysis. Speaker 4: And so we could group them together and assign super filer to them. One example is a sweet book, Zero Fila Debra Opa 11 or the one and Chino too, and also almost clustered together. So we proposed a super final name. Potesky and Potesky means I'm bear or simple. And we choose that because they have a reduced and streamlined genome. That's another common feature. [00:15:00] I'm Andrea and I, I have to say that, you know, looking into evolutionary relationships, it is, it is a moving target because as Tanya mentioned, especially for microbes and bacteria and like here, there's still so many, um, candidates that are out there for which we have no genomic information. So we definitely need way more sequences, um, to get a better idea of the evolutionary relationships of all the books. Your Nokia out there Speaker 6: [00:15:30] spectrum is a public affairs show about science on k a l x Berkeley. Our guests today are Tanya. Okay. And Chris Rink k you single cell genomics to find the relationships between hundreds of dark matter of microbes. Speaker 4: And can you speak to the current throughput? I would have thought that gathering up organisms in such extreme environments was really the time limiting factor. [00:16:00] But I suppose if you have this archive, other steps might end up taking a while. I will say the most time consuming step is really to to sort those single cells and then to lyse the single cells and amplify the genome and then of course to screen them for the, for genomes of interest for microbial like metagenomes [inaudible] that was a big part of the study. So actually getting the genomic information out of the single cells and if that can be even more streamlined than uh, and push to a higher or even more stupid level, I think [00:16:30] that will speed up the recovery of, of novel microbial dogmatic genomes quite a bit. Speaker 5: Well, we have a pretty sophisticated pipeline now at the JGI where we can do this at a fairly high throughput, but as Chris said, it still takes time and every sample is different. Every sample behaves different depending on what the properties of the samples are. You may have to be treated in a certain way to make it most successful for this application and other staff in the whole process that takes a long time is the key. The quality control [00:17:00] of the data. So the data is not as pretty as a sequencing data from an isolet genome where you get a perfect genome back and the sequence data that you get back is fairly, even the coverage covered all around the genome. Single cell data is messy. The amplification process introduces these artifacts and issues. It can introduce some error because you're making copies of a genome. Speaker 5: So errors can happen. You can also introduce what we call comeric rearrangement. That means that pieces of DNA [00:17:30] go together that shouldn't go together. Again, that happens during the amplification process. It's just the nature of the process. And on top of that, parts of the genome amplify nicely and other parts not so nice. So the overall sort of what we call sequence coverage is very uneven. So the data is difficult to deal with. We have specific assembly pipelines that we do. We do a sort of a digital normalization of the data before we even deal with the data, so it's not as nice. And then on top of that you can have contamination. So the whole process is very [00:18:00] prone to contamination. Imagine you only have one copy of a single cell, five Phantogram, one circle of DNA and any little piece of DNA that you have in that prep that sometimes as we know comes with the reagents. Speaker 5: Because reagents are not designed to deal with such low template molecules. They will call amplify, they will out-compete or compete with your template. So what you end up with in your sequence is your target and other stuff that was in was in the reagents or again, in your prep. We have very rigorous [00:18:30] process of cleaning everything. We you read a lot of things we sterilize, so we need to get rid of any DNA to not, um, to, to have a good quality genome in the end. And so that said, we have developed tools and pipelines at our institute now that specifically help us detect contamination. Sometimes it's not easy to detect it and then remove it. We want to make sure that the single cell genomes that we released at as single cell genome ABC are really ABC and not a plus x and [00:19:00] B plus k because accidentally something came along and contaminated the prep. And especially with candidate Fila, it's, it's fairly difficult to detect tech contamination because what would help us would be if we would have referenced genomes, we're actually generating this reference genome so we don't have a good reference to say, yeah, this is actually, that's our target organism and the rest is public contamination, so it's very tricky. Speaker 4: Are there other examples for [00:19:30] single cell sequencing being used on this many organisms Speaker 5: on this many organisms? No, not that I'm aware of. I know there's an effort underway and the h and p, the human microbiome project where they also identified there, they nicely call it the most wanted list, so they have the target organisms that are quite abundant in different microbiomes within the human body associated with the human body and they've been very successfully able to cultivate. A lot of them bring a lot of them in culture [00:20:00] and it may be easier for the h and p because we can mimic the conditions within the body a little bit better and more controlled. We know our body temperature and we know sort of what the middle year is in the different parts of our body. So it's a little bit easier to bring these organisms and culture than going to the hydrothermal vent and try and recreate these conditions which are extremely difficult to recreate. So that said, um, there are some that they are now targeting with single cell sequencing. So that's another large effort [00:20:30] that I know of that's specifically using single cell genomics to get at some of these reference genomes. Speaker 4: Can you get more out of this then? Sort of phylogenetic links? We found a few unique genomic features and one on one dimension is we found a recode. It's stopped caught on in, in two of those, a bacteria from the hot vans I mentioned earlier. And to give you a little bit of background, so, um, it's, we know the genetic information of each sale is and coded in its DNA, but in order to [00:21:00] make use of this genomic information, this genetic information has to be translated into proteins. And then proteins that could be enzymes that are employed in the metabolism to keep the cell going. And a dispensation is pretty universal between the three domains of life. The way it works, we have three basis in your DNA and three basis are called the core done. And each call is translated in the one amino acid. Speaker 4: So this way you'll build a chain of amino acids and then this chain is for a folder [00:21:30] and then you have your ready made protein. This call them triplet. This three basis also work for start and stop. So there are certain colons that tell the cell, okay, that's where you start a protein. And another called in to tell us the cell. So that's, that's where you enter prod and you're done with it. There are some slight variations, but in general does a universally called, is perceived between all three domains of life. And what we found was very interesting in two of those bacteria from the hot vans. Ah, those two caecilian bacteria, we found the [00:22:00] recording. So one of the accord on did not called for a stop code on anymore, but in the quarter's for an amino acid in that case, glycine. And that has never been seen before. Were you surprised by these results? Speaker 5: To us, they were surprising because they were unique and they were different. On the other hand, I have to say I'm not that surprised because we haven't, like Russ said, we haven't looked at heart yet and considering that we can only cultivate a few percent of all the microbial diversity that exists on this planet as far as, [00:22:30] as far as we know it, it's not that surprising that you find these novel functions and there's these unique features and novel genetic codes because it's really, it's a highly under-explored area. Speaker 4: It is very rewarding. But if you look in the future, um, how much is still out of the sequence? Of course we're interested in that. So we looked at all the files show diversity that's known, that's out there based on this, um, biomarkers that Tony mentioned earlier and we just compared it to the genomes that we have sequenced so far. And we really want [00:23:00] to know, so if you want to cover let's say about 50% of all the fall diversity that's out there, how many achievements do we still have to sequence and the number of the estimate was we need to sequence at least 16,004 more genomes Speaker 5: and this is a moving target. So this is as we know, diversity of today it and every day we sample my environments, we sequence them deeper and everyday our diversity estimates increase. So what we've done with these 201 it's the tip of the iceberg but it's a start. Speaker 4: [00:23:30] Well Tanya and Chris, thanks for joining us. Thanks for having us. Thanks for having us. Yeah. Speaker 6: [inaudible] that's what shows are archived on iTunes to you. We've queued a simple link for you. The link is tiny, url.com/calex Speaker 7: spectrum Speaker 8: irregular feature of spectrum is a calendar [00:24:00] of some of the science and technology related events happening in the bay area over the next two weeks. Here's Brad swift and Renee Rao here today. Majority tomorrow. Expanding technological inclusion, technological inclusion is not an issue for some of us. It is an issue for all of us. Mitchell Kapore, co-chair of [inaudible] center for social impact and a partner at Kapore capital. We'll moderate a panel discussion among the following [00:24:30] presenters, Jennifer r Guayle, executive director of Latino to Kimberly Bryant, founder of Black Girls Code Connie Mack Keebler, a venture capitalist with the collaborative fund. Vivek Wadhwa academic researcher, writer and entrepreneur here today. Majority tomorrow is free and open to everyone on a first come first seated basis. This is happening on the UC Berkeley campus in Soutar de Di Hall [inaudible] [00:25:00] Auditorium Monday October 7th at 4:00 PM Speaker 7: the second installment of the six part public lecture series, not on the test. The pleasure and uses of mathematics will be held this October 9th Dr. Keith Devlin will deliver a lecture on underlying mathematics in video games. Dr Devlin will show how casual video games that provide representation of mathematics enabled children and adults to learn basic mathematics by playing in the same way people [00:25:30] learn music by learning to play the piano. Professor Devlin is a mathematician at Stanford, a Co founder and president of Inner Tube Games and the math guy of NPR. The lecture will be held on October 9th at 7:00 PM in the Berkeley City College Auditorium located at 2050 Center street in Berkeley. The event is free and open to the public. Speaker 8: The Leonardo arts science evening rendezvous or laser is a lecture series with rotating barrier venues. October 9th there will be a laser [00:26:00] at UC Berkeley. Presenters include Zan Gill, a former NASA scientists, Jennifer Parker of UC Santa Cruz, Cheryl Leonard, a composer, Wayne Vitali, founding member of gamelons Sakara [inaudible]. This is Wednesday, October 9th from 6:30 PM to 9:00 PM on the UC Berkeley campus in barrels hall room 100 Speaker 7: how can we prevent information technology [00:26:30] from destroying the middle class? Jaron Lanier, is it computer scientists, Kim Poser, visual artist and author. October 14th linear will present his ideas on the impact of information technology on his two most recent books are title. You are not a gadget and who owns the future. The seminar will be held in Sue Taja, Dai Hall, but not auditorium on the UC Berkeley campus. Monday, October 14th from 11:00 AM to noon [00:27:00] and that with some science news headlines. Here's the Renee, the intergovernmental panel on climate change released part of its assessment report. Five last Friday. The more than 200 lead authors on their report included Lawrence Berkeley National Labs, Michael Warner and William Collins who had a chapters on longterm climate change productions and climate models. The report reinforces previous conclusions that over the next century, the continents will warm [00:27:30] with more hot extremes and fewer cold extremes. Precipitation patterns around the world will also continue changing. One-Arm Collins noted that climate models since the last report in 2007 have improved significantly as both data collection and mechanistic knowledge have grown using these models. Scientists made several projections of different scenarios for the best, worst and middling cases of continued greenhouse emissions. Speaker 7: [00:28:00] Two recent accomplishments by commercial space programs are notable. Orbital Sciences launched their sickness spacecraft on September 18th a top the company's rocket and Tara's from wallops island, Virginia. On September 28th the Cygnus dock did the international space station for the first time, a space x rocket carrying and Canadian satellite has launched from the California coast in a demonstration flight of a new Falcon rocket. The next generation. Rocket boasts [00:28:30] upgraded engines designed to improve performance and carry heavier payloads. The rocket is carrying a satellite dead kiss IOP, a project of the Canadian Space Agency and other partners. Once in orbit it will track space weather. Speaker 2: Mm mm mm. Mm Huh. Speaker 7: The music [00:29:00] heard during the show was written and produced by Alex Simon. Yeah. Speaker 3: Thank you for listening to spectrum. If you have comments about the show, please send them to us via email. Address is [inaudible] dot [inaudible] dot com Speaker 9: [inaudible]. See acast.com/privacy for privacy and opt-out information.

Women's Media Center Live with Robin Morgan
WMC Live #24: Kim Gandy, Suzanne Braun Levine, Kimberly Bryant. (Original Airdate 2/2/2013)

Women's Media Center Live with Robin Morgan

Play Episode Listen Later Feb 2, 2013 55:31


Robin demolishes the "clash of civilizations" with Islam, and speaks with Kim Gandy, president of the National Network to End Domestic Violence, on the Violence Against Women Act; Kimberly Bryant on teaching kids tech in "Black Girls Code;" and author Suzanne Braun Levine on women's second adulthood and journalism now.

Wanda's Picks
Wanda's Picks Radio Show: SFBFF, SF Ethnic Dance Fest

Wanda's Picks

Play Episode Listen Later Jun 15, 2012 140:00


Today we are joined by: Bill Doggett, scholar, archivist and curator and Andy Kimmel, Remembus; Doggett speaks about his upcoming program, "The Civil War@150 Years: the Negro Spiritual and the Undergraound Railroad," which includes a short film created by Kimmel, at the African American Museum and Library at Oakland, 14th Street @ MLK Jr. Way, Sat., June 16, 2012, 2 PM. Almost 50 years ago, James Baldwin, noted author and activist visited San Francisco's Bayview District to talk to the black youth about what they were experiencing there. From that visit came the film: Take this Hammer, KQED's cinéma vérité record of the visit. Caroline Dijckmeester, Dutch director, stumbles upon this footage and decides to see if she can find some of these young men. She responded with a short film in response which is screening with the original at The Luggage Store Gallery in San Francisco, June 19, 2012, 7:30 PM. James Lockett, one of the subjects also joins us to talk about HP then and now. As a part of a series, Wanda's Picks has been profiling choreographers for the 34th Annual SF Ethnic Dance Festival which continues this weekend three, June 16-17 at YBCA. We speak to Rasika Kumar, Abhinaya Dance Company, about the work, "Synergy," a collaboation between her south Asian or Indian dance company and San Jose Taiko. Naomi Diouf, Artistic Director of Diamano Coura West African Dance Company, shares what her company is presenting this year, closing weekend, June 30-July 1. Kimberly Bryant, founder, Black Girls CODE, talks about blacks and the digital divide, even more pronounced when one looks at the absence of women and girls. Sunday afternoon, June 17, as a part of the San Francisco Black Film Festival, June 15-17, 2012, her organization is hosting a free workshop for boys and girls. Visit www.sfbff.org and http://blackgirlscode.org/

Hanselminutes - Fresh Talk and Tech for Developers
Improving Diversity in Technology with Kimberly Bryant from BlackGirlsCode.com

Hanselminutes - Fresh Talk and Tech for Developers

Play Episode Listen Later Jan 25, 2012 33:21


Scott sits down with Kimberly Bryant, a Biotechnology/Engineering professional and also the founder of BlackGirlsCode. Do we need more diversity in technology? Why? Are kids getting exposed to engineering as an option, and if not, why not?

(BIT) Blacks In Technology
#BITTechTalk ep. 23 w/ Kimberly Bryant

(BIT) Blacks In Technology

Play Episode Listen Later Nov 18, 2011 48:27


In this episode Greg speaks with Kimberly Bryant, Biotechnology/Engineering professional and the founder of Black Girls Code, a program that is focused on introducing girls of color between the ages of 7-14 to the field of digital technology and computer programming with a focus on emerging entrepreneurial concepts.Follow Kim on Twitter: @BlackGirlsCode

(BIT) Blacks In Technology
#BITTechTalk ep. 23 w/ Kimberly Bryant

(BIT) Blacks In Technology

Play Episode Listen Later Nov 17, 2011 48:27


In this episode Greg speaks with Kimberly Bryant, Biotechnology/Engineering professional and the founder of Black Girls Code, a program that is focused on introducing girls of color between the ages of 7-14 to the field of digital technology and computer programming with a focus on emerging entrepreneurial concepts. Follow Kim on Twitter: @BlackGirlsCode