Podcasts about serine

ReferencesLife Sci . 2019 Sep 1;232:116612 NLM Review 2023. SERINE in disease: https://www.ncbi.nlm.nih.gov/books/NBK592681/J Neurochem. 2018 Aug;146(4):416-428Guerra, DJ. 2025. Unpublished lecturesBeethoven, LV 1808. Symphony VI.in F major, Op. 68, The Pastoralehttps://music.youtube.com/watch?v=Zv4PIFRpYcw&si=kFCqlA6cI9WInpAQMozart, WA 1791.The Magic Flute. K620. Full Operahttps://music.youtube.com/watch?v=irCQ9dig3qk&si=MwQgv7Aj4TL_Sx6jBetts, D. 1972 :Blue Sky. Allman Brothers. Eat a Peach. lp.https://music.youtube.com/watch?v=JSMubgZoL58&si=FMmI8skw_ry0sMgH

This episode goes over the compounds of N-acetylcysteine (NAC) and Glycine, and how they are used to make Glutathione. Also, we go over a couple of use cases for Glutathione. This is strictly for educational purposes. The information in this video is not meant as a substitute for professional medical advice.Please like, comment and share if you find value in this video. Also, please ask any questions you have about supplements. I'll do a video reply as soon as possible.Resources: https://pmc.ncbi.nlm.nih.gov/articles/PMC4684116/https://cen.acs.org/articles/82/i3/METHYLMERCURY-TOXICOLOGY-PROBED.html#:~:text=%22Glutathione%20can%20be%20pumped%20out,brain%20as%20methylmercury%2Dglutathione.%22https://pmc.ncbi.nlm.nih.gov/articles/PMC4540061/https://www.sciencedirect.com/science/article/pii/S0163725821001182https://www.sciencedirect.com/science/article/pii/S0163725821001182 Image Resource:https://pmc.ncbi.nlm.nih.gov/articles/PMC6635095/ https://www.researchgate.net/figure/Overview-of-de-novo-glutathione-synthesis-Reduced-glutathione-or-GSH-is-a-tripeptide_fig2_5690064 https://lpi.oregonstate.edu/book/export/html/71 https://www.nature.com/articles/hr2011133 https://en.wikipedia.org/wiki/Serine_hydroxymethyltransferase - https://www.sciencedirect.com/topics/agricultural-and-biological-sciences/methylenetetrahydrofolate-reductasehttps://chemtymology.co.uk/2020/11/27/cysteine-and-cystine/ https://fda.report/DailyMed/972f09a7-2aa1-4b1b-a6dc-2c4578a07803 https://www.thoughtco.com/definition-of-precipitate-604612 https://pubchem.ncbi.nlm.nih.gov/compound/Acetyl-p-benzoquinone-imine#section=3D-Conformer https://www.semanticscholar.org/paper/DRUG-METABOLISM-IN-THE-LIVER-Nguyen/4a29c6c4d433685a94b94602c012b8731cba5493https://pubchem.ncbi.nlm.nih.gov/compound/S-_Methylmercury_-L-Cysteine#section=2D-Structurehttps://keymerhealth.com/articles/seafood-mercury/https://onlinelibrary.wiley.com/doi/10.1155/2021/6564585 https://www.sciencedirect.com/science/article/abs/pii/S0304416519300133

Discuss my thoughts on whether Dimethylglycine or Trimethylglycine is "better" for Homocysteine conversion to Methionine.This is strictly for educational purposes. The information in this video is not meant as a substitute for professional medical advice.Please like, comment and share if you find value in this video. Also, please ask any questions you have about supplements. I'll do a video reply as soon as possible.Resources: https://pmc.ncbi.nlm.nih.gov/articles/PMC4684116/https://cen.acs.org/articles/82/i3/METHYLMERCURY-TOXICOLOGY-PROBED.html#:~:text=%22Glutathione%20can%20be%20pumped%20out,brain%20as%20methylmercury%2Dglutathione.%22https://pmc.ncbi.nlm.nih.gov/articles/PMC4540061/https://www.sciencedirect.com/science/article/pii/S0163725821001182https://www.sciencedirect.com/science/article/pii/S0163725821001182 Image Resource:https://pmc.ncbi.nlm.nih.gov/articles/PMC6635095/ https://www.researchgate.net/figure/Overview-of-de-novo-glutathione-synthesis-Reduced-glutathione-or-GSH-is-a-tripeptide_fig2_5690064 https://lpi.oregonstate.edu/book/export/html/71 https://www.nature.com/articles/hr2011133 https://en.wikipedia.org/wiki/Serine_hydroxymethyltransferase - https://www.sciencedirect.com/topics/agricultural-and-biological-sciences/methylenetetrahydrofolate-reductasehttps://chemtymology.co.uk/2020/11/27/cysteine-and-cystine/ https://fda.report/DailyMed/972f09a7-2aa1-4b1b-a6dc-2c4578a07803 https://www.thoughtco.com/definition-of-precipitate-604612 https://pubchem.ncbi.nlm.nih.gov/compound/Acetyl-p-benzoquinone-imine#section=3D-Conformer https://www.semanticscholar.org/paper/DRUG-METABOLISM-IN-THE-LIVER-Nguyen/4a29c6c4d433685a94b94602c012b8731cba5493https://pubchem.ncbi.nlm.nih.gov/compound/S-_Methylmercury_-L-Cysteine#section=2D-Structurehttps://keymerhealth.com/articles/seafood-mercury/https://onlinelibrary.wiley.com/doi/10.1155/2021/6564585 https://www.sciencedirect.com/science/article/abs/pii/S0304416519300133https://www.jbc.org/cms/10.1074/jbc.M111.265348/asset/822df563-eda2-4f48-a333-d01370a79e9c/main.assets/gr5_lrg.jpghttps://www.researchgate.net/figure/Betaine-metabolism-Metabolic-pathways-involving-betaine-BHMT-betaine-homocysteine_fig11_225081065https://www.acs.org/molecule-of-the-week/archive/c/l-cysteine.htmlhttps://biologydictionary.net/glutamic-acid/https://www.researchgate.net/figure/An-overview-of-the-reaction-catalyzed-by-SHMT-and-its-context-in-the-folate-cycle-A-The_fig1_338995871https://pubchem.ncbi.nlm.nih.gov/compound/5-Methyltetrahydrofolate#section=2D-Structurehttps://pubchem.ncbi.nlm.nih.gov/compound/Tetrahydrofolic-acid#section=2D-Structurehttps://pubchem.ncbi.nlm.nih.gov/compound/135398652#section=2D-Structure

C'est bientôt les vacances et en tant que grande voyageuse, Serine Ayari a quelques astuces pour nous Merci pour votre écoute Pour écouter TIPIK à tout moment : www.rtbf.be/tipik Retrouvez tous les épisodes du Meilleur du Réveil de Tipik sur notre plateforme Auvio.be : https://auvio.rtbf.be/emission/18841 Et si vous avez apprécié ce podcast, n'hésitez pas à nous donner des étoiles ou des commentaires, cela nous aide à le faire connaître plus largement.

BUFFALO, NY- June 5, 2024 – A new research paper was published in Aging (listed by MEDLINE/PubMed as "Aging (Albany NY)" and "Aging-US" by Web of Science) Volume 16, Issue 10, entitled, “Serine racemase expression profile in the prefrontal cortex and hippocampal subregions during aging in male and female rats.” Aging is associated with a decrease in N-methyl-D-aspartate (NMDA) receptor function, which is critical for maintaining synaptic plasticity, learning, and memory. Activation of the NMDA receptor requires binding of the neurotransmitter glutamate and also the presence of co-agonist D-serine at the glycine site. The enzymatic conversion of L-serine to D-serine is facilitated by the enzyme serine racemase (SR). Subsequently, SR plays a pivotal role in regulating NMDA receptor activity, thereby impacting synaptic plasticity and memory processes in the central nervous system. As such, age-related changes in the expression of SR could contribute to decreased NMDA receptor function. However, age-associated changes in SR expression levels in the medial and lateral prefrontal cortex (mPFC, lPFC), and in the dorsal hippocampal subfields, CA1, CA3, and dentate gyrus (DG), have not been thoroughly elucidated. In this new study, researchers Linda Bean, Prodip K. Bose, Asha Rani, and Ashok Kumar from Indiana University School of Medicine, North Florida/South Georgia Veterans Health System, and the University of Florida aimed to determine the SR expression profile, including protein levels and mRNA, for these regions in aged and young male and female Fischer-344 rats. Their results demonstrate a significant reduction in SR expression levels in the mPFC and all hippocampal subfields of aged rats compared to young rats. No sex differences were observed in the expression of SR. “These findings suggest that the decrease in SR levels may play a role in the age-associated reduction of NMDA receptor function in brain regions crucial for cognitive function and synaptic plasticity.” DOI - https://doi.org/10.18632/aging.205841 Corresponding author - Ashok Kumar - kash@ufl.edu Sign up for free Altmetric alerts about this article - https://aging.altmetric.com/details/email_updates?id=10.18632%2Faging.205841 Subscribe for free publication alerts from Aging - https://www.aging-us.com/subscribe-to-toc-alerts Keywords - aging, serine racemase, hippocampus, medial prefrontal cortex (mPFC), NMDA receptor About Aging-US Aging publishes research papers in all fields of aging research, including but not limited to aging processes (from yeast to mammals), cellular senescence, age-related diseases (such as cancer and Alzheimer's disease) and their prevention and treatment, anti-aging strategies and drug development, and, importantly, the role of signal transduction pathways in aging (such as mTOR) and potential approaches to modulate these signaling pathways to extend lifespan. The journal aims to promote 1) treatment of age-related diseases by slowing down aging, 2) validation of anti-aging drugs by treating age-related diseases, and 3) prevention of cancer by inhibiting aging. (Cancer and COVID-19 are age-related diseases.) Please visit our website at https://www.Aging-US.com​​ and connect with us: Facebook - https://www.facebook.com/AgingUS/ X - https://twitter.com/AgingJrnl Instagram - https://www.instagram.com/agingjrnl/ YouTube - https://www.youtube.com/@AgingJournal LinkedIn - https://www.linkedin.com/company/aging/ Pinterest - https://www.pinterest.com/AgingUS/ Spotify - https://open.spotify.com/show/1X4HQQgegjReaf6Mozn6Mc Media Contact 18009220957 MEDIA@IMPACTJOURNALS.COM

.Les astres l'ont dit, Serine Ayari va bientôt rencontrer le grand amour ! Merci pour votre écoute Pour écouter TIPIK à tout moment : www.rtbf.be/tipik Retrouvez tous les épisodes du Meilleur du Réveil de Tipik sur notre plateforme Auvio.be : https://auvio.rtbf.be/emission/18841 Et si vous avez apprécié ce podcast, n'hésitez pas à nous donner des étoiles ou des commentaires, cela nous aide à le faire connaître plus largement.

La fête de mères puis la fête des pères O.K... Mais quand est-ce qu'on célèbre les super tatas ? Merci pour votre écoute Pour écouter TIPIK à tout moment : www.rtbf.be/tipik Retrouvez tous les épisodes du Meilleur du Réveil de Tipik sur notre plateforme Auvio.be : https://auvio.rtbf.be/emission/18841 Et si vous avez apprécié ce podcast, n'hésitez pas à nous donner des étoiles ou des commentaires, cela nous aide à le faire connaître plus largement.

Serine Ayari fait son grand nettoyage de printemps Merci pour votre écoute Pour écouter TIPIK à tout moment : www.rtbf.be/tipik Retrouvez tous les épisodes du Meilleur du Réveil de Tipik sur notre plateforme Auvio.be : https://auvio.rtbf.be/emission/18841 Et si vous avez apprécié ce podcast, n'hésitez pas à nous donner des étoiles ou des commentaires, cela nous aide à le faire connaître plus largement.

Dans cet épisode, je reçois Serine Ayari, on va revenir sur sa carrière : ses débuts sur scène à NY, sa vision du métier d'humoriste, la radio, sa famille, son enfance ... ; sans oublier, le Quizz'art, mais également ses kiffs musiques, cinés, séries, tout ça et bien plus encore c'est Frat Pack.Retrouve des vidéos inédites sur les réseaux et Youtube (@alexandrezama & @fratpacklemedia)Bon épisode la famille !N'oublie pas de t'abonner pour être informé dès la sortie d'un nouvel épisode, tu peux commenter et noter ce podcast c'est toujours très important pour nous, merci d'avance ! Hébergé par Acast. Visitez acast.com/privacy pour plus d'informations.

Serine Ayari a quelques idées pour se lancer en politique. Faut-il vraiment la laisser faire ? Merci pour votre écoute Pour écouter TIPIK à tout moment : www.rtbf.be/tipik Retrouvez tous les épisodes du Meilleur du Réveil de Tipik sur notre plateforme Auvio.be : https://auvio.rtbf.be/emission/18841 Et si vous avez apprécié ce podcast, n'hésitez pas à nous donner des étoiles ou des commentaires, cela nous aide à le faire connaître plus largement.

Serine Ayari panique ! Elle manque de vitamine D... Merci pour votre écoute Pour écouter TIPIK à tout moment : www.rtbf.be/tipik Retrouvez tous les épisodes du Meilleur du Réveil de Tipik sur notre plateforme Auvio.be : https://auvio.rtbf.be/emission/18841 Et si vous avez apprécié ce podcast, n'hésitez pas à nous donner des étoiles ou des commentaires, cela nous aide à le faire connaître plus largement.

Serine découvre les pouvoirs insoupçonné des ampoules connectées... Merci pour votre écoute Pour écouter TIPIK à tout moment : www.rtbf.be/tipik Retrouvez tous les épisodes du Meilleur du Réveil de Tipik sur notre plateforme Auvio.be : https://auvio.rtbf.be/emission/18841 Et si vous avez apprécié ce podcast, n'hésitez pas à nous donner des étoiles ou des commentaires, cela nous aide à le faire connaître plus largement.

Kawtar en Keyaert stellen 22 Minuten Stomme Vragen aan Serine Ayari. Welke BV zat al eens in haar DM's? Waarom werd ze ooit verdacht als spion? En wat is haar mening over limoenen?

Drs. Jason Crowell and Subhojit Roy discuss evidence that suggests phosphorylated α-synuclein might actually have a role in normal brain cell function. Show Reference: https://www.cell.com/neuron/fulltext/S0896-6273(23)00894-2?_returnURL=https%3A%2F%2Flinkinghub.elsevier.com%2Fretrieve%2Fpii%2FS0896627323008942%3Fshowall%3Dtrue 

Dr. Jason Crowell talks with Dr. Subhojit Roy about a platform for investigating the role of serine-129 in synucleinopathies, with implications for drug development. Read the related article in the Neuron. Disclosures can be found at Neurology.org.

Serine Ayari et la Saint Valentin... Merci pour votre écoute Pour écouter TIPIK à tout moment : www.rtbf.be/tipik Retrouvez tous les épisodes du Meilleur du Réveil de Tipik sur notre plateforme Auvio.be : https://auvio.rtbf.be/emission/18841 Et si vous avez apprécié ce podcast, n'hésitez pas à nous donner des étoiles ou des commentaires, cela nous aide à le faire connaître plus largement.

Ce matin, Serine reconnaît avoir vieilli, puisque c'est maintenant à son tour de se plaindre de la hausse des prix et de dire "avant, c'était mieux". Merci pour votre écoute Pour écouter TIPIK à tout moment : www.rtbf.be/tipik Retrouvez tous les épisodes du Meilleur du Réveil de Tipik sur notre plateforme Auvio.be : https://auvio.rtbf.be/emission/18841 Et si vous avez apprécié ce podcast, n'hésitez pas à nous donner des étoiles ou des commentaires, cela nous aide à le faire connaître plus largement.

Sérine va très bien, elle nous explique pourquoi Merci pour votre écoute Pour écouter TIPIK à tout moment : www.rtbf.be/tipik Retrouvez tous les épisodes du Meilleur du Réveil de Tipik sur notre plateforme Auvio.be : https://auvio.rtbf.be/emission/18841 Et si vous avez apprécié ce podcast, n'hésitez pas à nous donner des étoiles ou des commentaires, cela nous aide à le faire connaître plus largement.

Pour Serine, New York ou l'aéroport, c'est presque pareil ! Merci pour votre écoute Pour écouter TIPIK à tout moment : www.rtbf.be/tipik Retrouvez tous les épisodes du Meilleur du Réveil de Tipik sur notre plateforme Auvio.be : https://auvio.rtbf.be/emission/18841 Et si vous avez apprécié ce podcast, n'hésitez pas à nous donner des étoiles ou des commentaires, cela nous aide à le faire connaître plus largement.

Serine a beaucoup hésité quant à où célébrer son nouvel an 2024… Elle a connu tellement de flops dans le passé !

Elle dort enfin dans son nouvel appartement. Serine a décidé de lister les meilleurs conseils pour devenir proprio, et éviter les galères.

In Nieuwe Normen ga ik elke week in gesprek met een BV die meedraagt aan de nieuwe normen van de Vlaamse maatschappij. Vandaag is Serine Ayari te gast. Je kent haar ongetwijfeld van De Slimte Mens Ter Wereld, Exen de serie en haar ongelooflijk grappige stand-up shows.

The Editor of the Milepost, Serine Reeves, joins Jennie to talk about road trips in Alaska, including her favorite places, road tripping with kids and what to bring on your road trip.The MilepostAlaska road conditions and construction updatesJennie's article about the best Alaska road tripsGet Jennie's Alaska Travel planners and pre made itinerariesBook a trip planning session with JennieGet Jennie's weekly Alaska Travel tips in your inboxFollow Jennie on InstagramLet Jennie plan your trip for you!Music credits:  Largo Montebello, by Domenico Mannelli, CC.

CME credits: 1.25 Valid until: 15-06-2024 Claim your CME credit at https://reachmd.com/programs/cme/the-neutrophil-and-neutrophil-serine-proteases-nsps-in-non-cf-bronchiectasis-ncfbe/15620/ An estimated 350,000-500,000 adults are affected by bronchiectasis in the United States, and up to 90% of patients with bronchiectasis present with chronic cough. This educational activity provides a framework of treatment options for NCFBE, empowering medical practitioners to work collaboratively with pulmonary specialists and confidently proceed with a sustainable treatment plan that is evidence-based and makes sense for both practitioner and patient.

La Virilos SERINE AYARI a bien voulu venir parler de sa carrière de standuppeuse, de ses vies d'avant, de ce qu'elle déteste, de ce que ça veut dire être une femme dans ce métier si étiqueté masculin qu'est l'humour. Et puis on a chié sur la cuisine italienne, et ça fait du bien. Bonne écoute ! Hébergé par Acast. Visitez acast.com/privacy pour plus d'informations.

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2023.04.24.538049v1?rss=1 Authors: Castano, A., Silvestre, M., Wells, C. I., Sanderson, J. L., Ferrer, C. A., Ong, H. W., Lang, Y., Richardson, W., Silvaroli, J. A., Bashore, F. M., Smith, J. L., Genereux, I. M., Dempster, K., Drewry, D. H., Pabla, N. S., Bullock, A. N., Benke, T. A., Ultanir, S., Axtman, A. D. Abstract: Pathological loss-of-function mutations in cyclin-dependent kinase-like 5 (CDKL5) cause CDKL5 deficiency disorder (CDD), a rare and severe neurodevelopmental disorder associated with severe and medically refractory early-life epilepsy, motor, cognitive, visual and autonomic disturbances in the absence of any structural brain pathology. Analysis of genetic variants in CDD have indicated that CDKL5 kinase function is central to disease pathology. CDKL5 encodes a serine-threonine kinase with significant homology to GSK3b, which has also been linked to synaptic function. Further, Cdkl5 knock-out rodents have increased GSK3b activity and often increased long-term potentiation (LTP). Thus, development of a specific CDKL5 inhibitor must be careful to exclude cross-talk with GSK3b activity. We synthesized and characterized specific, high-affinity inhibitors of CDKL5 that do not have detectable activity for GSK3b. These compounds are very soluble in water but blood-brain barrier penetration is low. In rat hippocampal brain slices, acute inhibition of CDKL5 selectively reduces post-synaptic function of AMPA-type glutamate receptors in a dose-dependent manner. Acute inhibition of CDKL5 reduces hippocampal LTP. These studies provide new tools and insights into the role of CDKL5 as a newly appreciated, key kinase necessary for synaptic plasticity. Comparisons to rodent knock-out studies suggest that compensatory changes have limited the understanding of the roles of CDKL5 in synaptic physiology, plasticity and human neuropathology. Copy rights belong to original authors. Visit the link for more info Podcast created by Paper Player, LLC

A new research paper was published in Aging (Aging-US) Volume 15, Issue 7, entitled, “Viral vector-mediated upregulation of serine racemase expression in medial prefrontal cortex improves learning and synaptic function in middle age rats.” An age-associated decrease in N-methyl-D-aspartate receptor (NMDAR)-mediated synaptic function contributes to impaired synaptic plasticity and is associated with cognitive impairments. Levels of serine racemase (SR), an enzyme that synthesizes D-serine, an NMDAR co-agonist, decline with age. In this new study, researchers Brittney Yegla, Asha Rani and Ashok Kumar from the University of Florida's McKnight Brain Institute predicted that enhancing NMDAR function via increased SR expression in middle age (when subtle declines in cognition emerge) may enhance performance on a prefrontal cortex-mediated task sensitive to aging. “We hypothesized that augmenting SR expression within mPFC glutamatergic neurons would improve attention and cognitive flexibility in middle-aged rats and facilitate synaptic responses in the mPFC. Thus, for this study, SR expression was upregulated in pyramidal neurons of the mPFC through lenti-viral technology to enhance NMDAR function and evaluate its impact on cognitive flexibility and NMDAR-mediated synaptic transmission in middle-age rats.” Middle-aged (~12 mo) male Fischer-344 rats were injected bilaterally in the medial prefrontal cortex (mPFC) with viral vector (LV), SR (LV-SR) or control (LV-GFP). Rats were trained on the operant attentional set-shift task (AST) to examine cognitive flexibility and attentional function. LV-SR rats exhibited a faster rate of learning compared to controls during visual discrimination of the AST. Extradimensional set shifting and reversal were not impacted. Immunohistochemical analyses demonstrated that LV-SR significantly increased SR expression in the mPFC. Electrophysiological characterization of synaptic transmission in the mPFC slices obtained from LV-GFP and LV-SR animals indicated a significant increase in isolated NMDAR-mediated synaptic responses in LV-SR slices. Thus, results of the current study demonstrated that prefrontal SR upregulation in middle age rats can improve learning of task contingencies for visual discrimination and increase glutamatergic synaptic transmission, including NMDAR activity. “The results from this study support the beneficial effects of the D-serine pathway involvement in NMDAR-mediated transmission and cognitive function, expanding the literature to emphasize its role in not only the hippocampus but also the PFC. Thus, targeting this pathway could pose a potential route in reversing age-related cognitive decline and should be considered for future research.” DOI: https://doi.org/10.18632/aging.204652 Corresponding Author: Ashok Kumar - kash@ufl.edu Keywords - aging, medial prefrontal cortex, serine racemase, D-serine, NMDA receptor, cognitive flexibility About Aging-US Launched in 2009, Aging-US publishes papers of general interest and biological significance in all fields of aging research and age-related diseases, including cancer—and now, with a special focus on COVID-19 vulnerability as an age-dependent syndrome. Topics in Aging-US go beyond traditional gerontology, including, but not limited to, cellular and molecular biology, human age-related diseases, pathology in model organisms, signal transduction pathways (e.g., p53, sirtuins, and PI-3K/AKT/mTOR, among others), and approaches to modulating these signaling pathways. Please visit our website at https://www.Aging-US.com​​ and connect with us: SoundCloud - https://soundcloud.com/Aging-Us Facebook - https://www.facebook.com/AgingUS/ Twitter - https://twitter.com/AgingJrnl Instagram - https://www.instagram.com/agingjrnl/ YouTube - https://www.youtube.com/@AgingJournal LinkedIn - https://www.linkedin.com/company/aging/ Pinterest - https://www.pinterest.com/AgingUS/ MEDIA@IMPACTJOURNALS.COM

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2023.04.19.537136v1?rss=1 Authors: Osorio, N., Martineau, M., Fortea, M., Rouget, C., Penalba, V., Lee, C. J., Boesmans, W., Rolli-Derkinderen, M., Patel, A. V., Mondielli, G., Conrod, S., Labat-Gest, V., Papin, A., Sasabe, J., Sweedler, J. V., Vanden Berghe, P., Delmas, P., MOTHET, J.-P. Abstract: The enteric nervous system (ENS) is a complex network of diverse molecularly defined classes of neurons embedded in the gastrointestinal wall and responsible for controlling the major functions of the gut. As in the central nervous system, the vast array of ENS neurons is interconnected by chemical synapses. Despite several studies reporting the expression of ionotropic glutamate receptors in the ENS, their roles in the gut remain elusive. Here, by using an array of immunohistochemistry, molecular profiling and functional assays, we uncover a new role for D-serine (D-Ser) and non-conventional GluN1-GluN3 N-methyl D-aspartate receptors (NMDARs) in regulating ENS functions. We demonstrate that D-Ser is produced by serine racemase (SR) expressed in enteric neurons. By using both in situ patch clamp recording and calcium imaging, we show that D-Ser alone acts as an excitatory neurotransmitter in the ENS independently of the conventional GluN1-GluN2 NMDARs. Instead, D-Ser directly gates the non-conventional GluN1-GluN3 NMDARs in enteric neurons from both mouse and guinea-pig. Pharmacological inhibition or potentiation of GluN1-GluN3 NMDARs had opposite effects on mouse colonic motor activities, while genetically driven loss of SR impairs gut transit and fluid content of pellet output. Our results demonstrate the existence of native GluN1-GluN3 NMDARs in enteric neurons and open new perspectives on the exploration of excitatory D-Ser receptors in gut function and diseases. Copy rights belong to original authors. Visit the link for more info Podcast created by Paper Player, LLC

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2023.03.23.533952v1?rss=1 Authors: Jackson, C. B., Marmyleva, A., Awadhpersad, R., Monteuuis, G., Mito, T., Zamboni, N., Tatsuta, T., Vincent, A. E., Wang, L., Langer, T., Carroll, C. J., Suomalainen-Wartiovaara, A. Abstract: Importance of serine as a metabolic regulator is well known in tumors and raising attention also in degenerative diseases. Recent data indicate that de novo serine biosynthesis is an integral component of metabolic response to mitochondrial disease, but the roles of the response have remained unknown. Here, we report that glucose-driven de novo serine biosynthesis maintains metabolic homeostasis in energetic stress. Pharmacological inhibition of the rate-limiting enzyme, phosphoglycerate dehydrogenase (PHGDH), aggravated mitochondrial muscle disease, suppressed oxidative phosphorylation and mitochondrial translation, altered whole-cell lipid profiles and enhanced mitochondrial integrated stress response (ISRmt), in vivo, in skeletal muscle and in cultured cells. Our evidence indicates that de novo serine biosynthesis is essential to maintain mitochondrial respiration, redox balance, and cellular lipid homeostasis in skeletal muscle with mitochondrial dysfunction. Our evidence implies that interventions activating de novo serine synthesis may protect against mitochondrial failure in the skeletal muscle. Copy rights belong to original authors. Visit the link for more info Podcast created by Paper Player, LLC

Today's episode is a bit different - Chelsea sits in the guest seat as her online bestie Serine Goodmond fills in as host! Chelsea just rebranded, and Serine is here to peel back the layers of Chelsea's origin story to understand more context around this rebrand, and where Chelsea is headed next. Tune in to hear: - where Chelsea's love of storytelling came from - why she's obsessed with creating and sharing stories - her journey to podcasting - how podcast coaching and strategy came to be her main focus - funny life stories that are now synchronicities - Chelsea's current focus and obsessions - Chelsea's audio world predictions CONNECT WITH SERINE: www.serinegoodmond.com CONNECT WITH CHELSEA: www.chelseariffe.com @chelseariffe on Instagram AMPLIFY Mastermind - starts February 2023 - 6 spaces open! 1:1 Coaching- waitlist to start in March 2023 Mic Drop Waitlist Leave a voice note to the podcast HERE. Full transcript of episode can be found HERE.

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2023.01.11.523603v1?rss=1 Authors: Ziołkowska, M., Borczyk, M., Cały, A., Nalberczak-Skora, M., Nowacka, A., Sliwinska, M. A., Łukasiewicz, K., Skonieczna, E., Tomaszewski, K. F., Wojtowicz, T., Wlodarczyk, J., Bernas, T., Salamian, A., Radwanska, K. Abstract: The updating of contextual memories is essential for survival in a changing environment. Accumulating data indicate that the dorsal CA1 area (dCA1) contributes to this process. However, the cellular and molecular mechanisms of contextual fear memory updating remain poorly understood. Postsynaptic density protein 95 (PSD-95) regulates the structure and function of glutamatergic synapses. Here, using dCA1-targeted genetic manipulations in vivo, combined with ex vivo 3D electron microscopy and electrophysiology, we identify a novel, synaptic mechanism that is induced during attenuation of contextual fear memories and involves phosphorylation of PSD-95 at Serine 73 in dCA1. Our data provide the proof that PSD-95-dependent synaptic plasticity in dCA1 is required for updating of contextual fear memory. Copy rights belong to original authors. Visit the link for more info Podcast created by Paper Player, LLC

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2022.12.22.521485v1?rss=1 Authors: Parra-Rivas, L. A., Madhivanan, K., Wang, L., Boyer, N. P., Prakashchand, D. D., Aulston, B. D., Pizzo, D. P., Branes-Guerrero, K., Tang, Y., Das, U., Scott, D. A., Rangamani, P., Roy, S. Abstract: Phosphorylation of -synuclein at the Serine-129 site (-syn Ser129P) is an established pathologic hallmark of synucleinopathies, and also a therapeutic target. In physiologic states, only a small fraction of total -syn is phosphorylated at this site, and consequently, almost all studies to date have focused on putative pathologic roles of this post-translational modification. We noticed that unlike native (total) -syn that is widely expressed throughout the brain, the overall pattern of -syn Ser129P is restricted, suggesting intrinsic regulation and putative physiologic roles. Surprisingly, preventing phosphorylation at the Ser-129 site blocked the ability of -syn to attenuate activity-dependent synaptic vesicle (SV) recycling; widely thought to reflect its normal function. Exploring mechanisms, we found that neuronal activity augments -syn Ser-129P, and this phosphorylation is required for -syn binding to VAMP2 and synapsin - two functional binding-partners that are necessary for -syn function. AlphaFold2-driven modeling suggests a scenario where Ser129P induces conformational changes in the C-terminus that stabilizes this region and facilitates protein-protein interactions. Our experiments indicate that the pathology-associated Ser129P is an unexpected physiologic trigger of -syn function, which has broad implications for pathophysiology and drug-development. Copy rights belong to original authors. Visit the link for more info Podcast created by Paper Player, LLC

Sérine Ayari est originaire de Flandre, elle est connue pour son franc parler légendaire. A voir s'il y a un lien de cause à effet. Chaque mercredi elle nous parle d'un sujet qui la chiffonne.. Accrochez-vous, Sérine elle dit souvent tout haut ce que tout le monde pense tout bas..

Pierre Scheurette est de retour pour une nouvelle saison dans le Réveil de Tipik avec Audrey, Lara, Marco et François ! Journaliste, chroniqueur, auteur, Pierre a une vie bien remplie. Entre l'actualité qui part en vrille et sa vie bien chargée, Pierre nous partage chaque semaine sa cueillette de la semaine. "Aujourd'hui, il faut que je vous raconte un peu les coulisses. Normalement je devais venir jeudi, Serine Ayari m'a demandé d'échanger de jour… Au début ça m'arrangeait pas trop, donc j'ai un peu hésité, et puis quand j'ai vu quel jour on était, j'ai dit oui ! 21 décembre ! C'est la journée mondiale de l'orgasme ! J'étais obligé d'y aller ! Mais pas de panique, on va étudier le sujet de manière quasi encyclopédique. Donc sachez le : ça tombe tous les 21 décembre, la journée mondiale de l'orgasme… Bon… Sauf pour ma meuf, chez qui ça tombe plutôt tous les 30 février. La journée mondiale de l'orgasme qui est l'équivalent de la Fête du Travail pour Clara Morgane et qui est une journée que les éjaculateurs précoces, eux, ont déjà fêté hier, évidemment."

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2022.12.05.519070v1?rss=1 Authors: Cariulo, C., Martufi, P., Verani, M., Toledo, L. S., Lee, R., Dominguez, C., Petricca, L., Caricasole, A. Abstract: N-terminal phosphorylation at residues T3 and S13 is believed to have important beneficial implications for the biological and pathological properties of mutant huntingtin, where IKBKB was identified as a candidate regulator of huntingtin N-terminal phosphorylation. The paucity of mechanistic information on IKK pathways, together with the lack of sensitive methods to quantify endogenous huntingtin phosphorylation, prevented detailed study of the role of IKBKB in Huntington's disease. Using novel ultrasensitive assays, we demonstrate that IKBKB can regulate endogenous S13 huntingtin phosphorylation in a manner dependent on its kinase activity and known regulators. We found that the ability of IKBKB to phosphorylate endogenous huntingtin S13 is mediated through a non-canonical IRF3-mediated IKK-pathway, distinct from the established involvement of IKBKB in mutant huntingtin's pathological mechanisms mediated via the canonical pathway. Furthermore, increased huntingtin S13 phosphorylation by IKBKB resulted in decreased aggregation of mutant huntingtin in cells, again dependent on its kinase activity. These findings point to a non-canonical IKK-pathway linking S13 huntingtin phosphorylation to the pathological properties of mutant huntingtin aggregation, thought to be significant to Huntington's disease. Copy rights belong to original authors. Visit the link for more info Podcast created by Paper Player, LLC

Sérine Ayari est originaire de Flandre, elle est connue pour son franc parler légendaire. A voir s'il y a un lien de cause à effet. Chaque mercredi elle nous parle d'un sujet qui la chiffonne.. Accrochez-vous, Sérine elle dit souvent tout haut ce que tout le monde pense tout bas..

Et cette semaine, faites du bruit (où que vous soyez) pour Serine Ayari !Et pour ne pas manquer les prochains épisodes de Montreux Comedy Edition Audio, abonnez-vous sur vos plateformes de podcasts préférées !Suivez-nous partout !- YouTube : bit.ly/YoutubeMCF- Facebook : bit.ly/FacebookMCF- Instagram : bit.ly/InstagramMCF- Twitter : bit.ly/TwitterMCF- TikTok : bit.ly/TikTokMCFEt aussi sur montreuxcomedy.com Hébergé par Acast. Visitez acast.com/privacy pour plus d'informations.

Aujourd'hui, le livre qu'on croque, c'est De Capes et de mots, un roman jeunesse de Flore Vesco, publié aux éditions Didier Jeunesse.On suit les aventures de Serine, une adolescente fougueuse, inventive et avec un bon sens de l'humour, dans ses entrées à la cour en tant que demoiselle de compagnie. Amatrice et amateur de jolis mots, jetez vous sur ce roman doux et sympathique !Pour me contacter, n'hésitez pas à m'envoyer un mail à lacroqueusedelivres@gresille.orgVos retours me sont précieux et n'hésitez pas à me donner vos recommandations de lecture par la même occasion !***Livres mentionnés dans l'épisode :D'or et d'oreillers, de Flore Vesco (il est super, lisez le !), éditions L'Ecole des Loisirs (2021)De Capes et de mots, de Flore Vesco, éditions Didier Jeunesse (2015)***Musique du générique :Credits: Not The King - Ice Tea - Royalty Free Vlog Music — Music By Not The KingMusique d'ambiance pour l'extrait lu: Video Game Blockbuster About the song Written by Rafael Krux. This music is available for commercial and non-commercial purposes. 100% Free Music - Free for Commercial Use, Free Of Royalties, Free Of Attribution, Creative Commons 0.***A bientôt, pour découvrir un nouveau livre à croquer… ou à dévorer !

https://youtu.be/V9z0MP1qZi0******Paula's Choice Skincare Omega+ Complex Moisturizer At Paula's Choice:https://paulachoiceusca.l3km.net/XxO4RMPaula's Choice Skincare Omega+ Complex Moisturizer Full Ingredients List:Water⁠, Butyrospermum Parkii Butter⁠, Ceteareth-6 Olivate⁠, Butylene Glycol⁠, C13-15 Alkane⁠, Glycerin⁠, Glyceryl Stearate⁠, Polyglyceryl-4 Laurate⁠, Decyl Oleate⁠, Linum Usitatissimum (Linseed) Seed Oil⁠, Salvia Hispanica (Chia) Seed Oil⁠, Olea Europaea (Olive) Fruit Oil⁠, Passiflora Edulis Seed Oil⁠, Borago Officinalis Seed Oil⁠, Hydrogenated Olive Oil⁠, Psidium Guajava Fruit Extract⁠, Olea Europaea Oil Unsaponifiables⁠, Limnanthes Alba (Meadowfoam) Seed Oil⁠, Linoleic Acid⁠, Linolenic Acid⁠, Oleic Acid⁠, Euterpe Oleracea (Acai) Sterols⁠, Ceramide AP⁠, Ceramide EOP⁠, Ceramide NP⁠, Serine⁠, Alanine⁠, Glycine⁠, Proline⁠, Lysine HCI⁠, Threonine⁠, Arginine⁠, Squalane⁠, Cholesterol⁠, Phytosphingosine⁠, Anastatica Hierochuntica Extract⁠, Lecithin⁠, Sodium Hyaluronate⁠, Sodium Lactate⁠, Boerhavia Diffusa Root Extract⁠, Adenosine⁠, Phytic Acid⁠, Glutamic Acid⁠, Betaine⁠, Pullulan⁠, PCA⁠, Sodium PCA⁠, Carbomer⁠, Sodium Polyacrylate Starch⁠, Sclerotium Gum⁠, Xanthan Gum⁠, Sodium Lauroyl Lactylate⁠, Phenoxyethanol⁠, Ethylhexylglycerin⁠ *****My second channel, for random things that don't fit here. https://www.youtube.com/channel/UCZwJQwh2qHT9qUrseCNasHg*******Podcast LinksApple - https://b.link/No_BS_Apple_PodcastGoogle - https://b.link/No_BS_Google_PodcastAmazon - https://b.link/No_BS_Amazon_PodcastSpotify - https://b.link/No_BS_Spotify_PodcastRSS - https://feeds.redcircle.com/671dd1b2-a989-41d5-94d5-30c014e06149********Sephora - https://fxo.co/1231867/sephoraUlta - https://fxo.co/1231867/ultaGeek and Gorgeous - https://iaff.geekandgorgeous.com/idevaffiliate.php?id=169&url=131 Amazon - https://www.amazon.com/shop/nobsbeautyYes Style - https://ys.style/kk2Vjrv798Style Korean - http://www.stylekorean.com/?af_id2=nobsbeautyDrmtlgy - https://www.drmtlgy.com/?ref=nobsbeautyThese are affiliate links if you purchase anything from one of these stores using this link No BS Beauty will make a small commission on what you buy.********www.noBSbeauty.com*******My Patreon - https://www.patreon.com/noBSbeauty*******PayPal Tip Jar - https://bit.ly/donate_NBSBIf you want to leave a tip ... Thanks! *****

Are you someone who regrets investing in a business coach, so much so that it keeps you up at night, and you can't believe what you paid for? Are you tired of spending your life savings and not getting the results you want for your biz? Is it really on you, or is it because the business coaching industry has a few cracks that need to be sealed, like yesterday? On this week's episode, Chelsea sits down with Serine Goodmond to discuss: “The Big Boom” in the business coaching industry - where did this demand even come from? Marketing your dollar signs - this is worsening capitalism in the business coaching industry Issues with business coaches - and the shocking results from Chelsea's Instagram poll (given by business owners who hired business coaches themselves!) Personal experiences - what worked out and didn't. Serine and Chelsea don't hold back. Spotting red flags - how to use discernment to avoid being straight up scammed How clients also play a part in their expectations falling short, and how to speak up for what they really need and want “We're in this American culture where we want things to be fixed instantaneously, you then have all these people who wanna work for themselves tomorrow and they need someone that's gonna help them get there yesterday…I think what we're seeing if we zoom out is that people really need to divest from capitalism as the baseline business structure. There's a huge over emphasis on money and income, and not the actual service that we're providing to our clients.” - Serine Goodmond CONNECT WITH SERINE: Instagram: https://www.instagram.com/sista.boss/?hl=en Website: https://www.shiftfromshame.com/ Join the next Daydream session here: https://www.shiftfromshame.com/daydream CONNECT WITH CHELSEA: Website: www.chelseariffe.com Instagram: https://www.instagram.com/chelseariffe/ 1:1 Podcast Coaching: https://www.chelseariffe.com/1-1-podcast-coaching Mic Drop Podcast Launching Course: https://www.chelseariffe.com/micdropcourse (EARLY BIRD CODE: MDEARLYBIRD for $100 off!) SUBMIT A QUESTION OR LEAVE A VOICE MESSAGE FOR THE PODCAST (NEW)! This episode is sponsored by BetterHelp. Get 10% off your first month of therapy here, or use code "riffe10" at check-out!

Chelsea sits down with her friend, Serine Goodmond, and gets into a deep conversation about social media, coaching, leadership, marketing, and more. Why are we spending so much time on Instagram? How can we stop centering ourselves in our marketing? How can we be better leaders online and in our spaces? These are just a few of the questions they get into in today's juicy ep! CONNECT WITH SERINE: www.shiftfromshame.com CONNECT WITH CHELSEA: Website: chelseariffe.com @chelseariffe + @nonexpertopinionpod Email: info@chelseariffe.com Work with Chelsea: https://linktr.ee/Chelseariffe Want to launch your own podcast? Chelsea's 1:1 podcast coaching is open until April 8th! Find more info and apply right HERE. Try Curious Elixirs booze-free cocktails! Get $10 off an order of $50 or more with code RIFFE22, or click HERE. Take advantage of the O-P-E-N app and join the April Challenge right HERE.

NIOD Modulating Glucosides Facial SerumAt SkinStore: https://fxo.co/DNs0NIOD Modulating Glucosides Full Ingredients List:Aqua (Water), Squalane, Isodecyl Neopentanoate, Glycerin, Caprylic/Capric Triglyceride, Pentylene Glycol, Propanediol, Hexyldecanol, Bisabolol, Butylene Glycol, Epigallocatechin Gallatyl Glucoside, Rosmarinyl Glucoside, Caffeyl Glucoside, Gallyl Glucoside, Tetrasodium Tetracarboxymethyl Naringeninchalcone, Hydroxymethoxyphenyl Decanone, 4-T-Butylcyclohexanol, Cetylhydroxyproline Palmitamide, Hydroxyphenyl Propamidobenzoic Acid, Palmitoyl Tripeptide-8, Superoxide Dismutase, Sodium Pca, Pca, Arginine, Glycine, Alanine, Serine, Valine, Isoleucine, Proline, Threonine, Histidine, Phenylalanine, Aspartic Acid, Sodium Lactate, Mirabilis Jalapa Callus Extract, Tasmannia Lanceolata Fruit/Leaf Extract, Zingiber Officinale (Ginger) Root Extract, Curculigo Orchioides Root Extract, Isochrysis Galbana Extract, Brassica Campestris (Rapeseed) Sterols, Stearic Acid, Dextran, Isoceteth-20, Polyacrylate Crosspolymer-6, Xanthan Gum, Tocopherol, Propyl Gallate, Tromethamine, Dehydroacetic Acid, 1,2-Hexanediol, Benzyl Alcohol, Phenoxyethanol, Caprylyl Glycol.*****I'm launching a second channel in 2022 to post some of the things that don't really fit with No BS Beauty. I'm not planning on posting immediately but wanted to let you know if you want to subscribe so you won't miss anything. https://www.youtube.com/channel/UCZwJQwh2qHT9qUrseCNasHg*******Podcast LinksApple - https://b.link/No_BS_Apple_PodcastGoogle - https://b.link/No_BS_Google_PodcastAmazon - https://b.link/No_BS_Amazon_PodcastSpotify - https://b.link/No_BS_Spotify_PodcastStitcher - https://b.link/No_BS_Stitcher_PodcastRSS - https://feeds.redcircle.com/671dd1b2-a989-41d5-94d5-30c014e06149********Sephora - https://fxo.co/1231867/sephoraUlta - https://fxo.co/1231867/ultaAmazon - https://www.amazon.com/shop/nobsbeautyYes Style - https://ys.style/kk2Vjrv798Style Korean - http://www.stylekorean.com/?af_id2=nobsbeautyThese are affiliate links if you purchase anything from one of these stores using this link No BS Beauty will make a small commission on what you buy.********I am proud to offer my very own beauty products at Amazon. We are starting small but hope to grow these offerings. Take a look and if you can pick one or two up, it helps keep this channel truly independent.My Products:No BS Beauty Travel Set - https://amzn.to/2PgPzFZNo BS Beauty Airless Jars - https://bit.ly/2Ev6X6N or https://amzn.to/2RCEq4sNo BS Beauty Color Switcher - https://amzn.to/2RCEAJ6See my own page on Amazon - https://www.amazon.com/shop/nobsbeautywww.noBSbeauty.net*******My Patreon - https://www.patreon.com/noBSbeauty*******PayPal Tip Jar - https://bit.ly/donate_NBSBIf you want to leave a tip ... Thanks! *****

References Infect Immun. 2018 Jul; 86(7): e00035-18 J Biol Chem. 2019 Jun 7;294(23):9213-9224 Egyptian Journal of Basic and Applied Sciences. 2018. (5) 4 : 237-244 Arteriosclerosis, Thrombosis, and Vascular Biology. 2019. (39) 3: 432-445 --- Send in a voice message: https://anchor.fm/dr-daniel-j-guerra/message Support this podcast: https://anchor.fm/dr-daniel-j-guerra/support

In this week's episode, Bonnie Galam chats with attorney Trevor Serine about how asset protection tools play out during a lawsuit. We cover things from offshore account to the insider threat no defensive tool can protect you from.In this episode you'll learn: Why LLCs sometimes provide limited asset protection in the event of a lawsuitWhat type of defensive asset protection is most protectiveWhat you can do to improve your chances of winning a future lawsuit today

Watch this at YouTube - https://youtu.be/gVR14DMm_D0Hyggee Natural Repair Serum at yesstyle: https://ys.style/tmRXARN7gkbUse Rewards Code NOBSBEAUTY for an additional discount!Hyggee Natural Repair Serum Full Ingredients List:Panax Ginseng Root Extract, Glycerin, Propanediol, 1,2-Hexanediol, Butylene Glycol, Niacinamide, Water, Simethicone, Panthenol, Gelidium Cartilagineum Extract, Laminaria Japonica Extract, Hizikia Fusiforme Extract, Ascophyllum Nodosum Extract, Ascorbic Acid Polypeptide, Lactobacillus Ferment, Glycyrrhiza Glabra (Licorice) Root Extract, Dextrin, Centella Asiatica Extract, Scutellaria Baicalensis Root Extract, Glycine, Polygonum Cuspidatum Root Extract, Serine, Glutamic Acid, Sesamum Indicum (Sesame) Seed Extract, Sophora Flavescens Root Extract, Camellia Sinensis Leaf Extract, Cimicifuga Racemosa Root Extract, Morus Alba Bark Extract, Paeonia Lactiflora Root Extract, Phellinus Linteus Extract, Angelica Gigas Root Extract, Polygonum Multiflorum Root Extract, Aspartic Acid, Leucine, Rosmarinus Officinalis (Rosemary) Leaf Extract, Chamomilla Recutita (Matricaria) Flower Extract, Paeonia Suffruticosa Root Extract, Alanine, Lysine, Arginine, Tyrosine, Phenylalanine, Valine, Pancratium Maritimum Extract, Threonine, Proline, Isoleucine, Histidine, Methionine, Cysteine, Cellulose Gum, Ammonium Acryloyldimethyltaurate/VP Copolymer, Sodium Polyacryloyldimethyl Taurate, Hydrogenated Polydecene, Ethylhexylglycerin, Adenosine, Trideceth-10, Theobroma Cacao (Cocoa) Extract, Disodium EDTA, t-Butyl Alcohol, Carbomer, Polyglutamic Acid, PVM/MA Copolymer, Betaine*******Podcast LinksApple - https://b.link/No_BS_Apple_PodcastGoogle - https://b.link/No_BS_Google_PodcastAmazon - https://b.link/No_BS_Amazon_PodcastSpotify - https://b.link/No_BS_Spotify_PodcastStitcher - https://b.link/No_BS_Stitcher_PodcastRSS - https://feeds.redcircle.com/671dd1b2-a989-41d5-94d5-30c014e06149********Sephora - https://fxo.co/1231867/sephoraUlta - https://fxo.co/1231867/ultaAmazon - https://www.amazon.com/shop/nobsbeautyYes Style - https://ys.style/kk2Vjrv798Style Korean - http://www.stylekorean.com/?af_id2=nobsbeautyThese are affiliate links if you purchase anything from one of these stores using this link No BS Beauty will make a small commission on what you buy.********I am proud to offer my very own beauty products at Amazon. We are starting small but hope to grow these offerings. Take a look and if you can pick one or two up, it helps keep this channel truly independent.My Products:No BS Beauty Travel Set - https://amzn.to/2PgPzFZNo BS Beauty Airless Jars - https://bit.ly/2Ev6X6N or https://amzn.to/2RCEq4sNo BS Beauty Color Switcher - https://amzn.to/2RCEAJ6See my own page on Amazon - https://www.amazon.com/shop/nobsbeautywww.noBSbeauty.net*******My Patreon - https://www.patreon.com/noBSbeauty*******PayPal Tip Jar - https://bit.ly/donate_NBSBIf you want to leave a tip ... Thanks! *****

Watch on YouTube - https://youtu.be/CeDDa7e_YdgProduct Link at Sephora - https://fxo.co/BtzaSummer Fridays Cloud Dew Oil Free Moisturizer Full Ingredients List:Water/Aqua/Eau, Propanediol, Dicaprylyl Carbonate, Glycerin, Pentylene Glycol, Sodium Hyaluronate, Hydrolyzed Sodium Hyaluronate, Glycine, Serine, Glutamic Acid, Aspartic Acid, Leucine, Alanine, Lysine, Arginine, Tyrosine, Phenylalanine, Proline, Threonine, Valine, Isoleucine, Histidine, Ascorbic Acid, Saccharide Isomerate, Haematococcus Pluvialis Extract, Ceramide NP, Tocopheryl Acetate, Tocopherol, Hexapeptide-11, Palmitoyl Tripeptide-1, Ananas Sativus (Pineapple) Fruit Extract, Saccharomyces/Magnesium Ferment, Saccharomyces/Copper Ferment, Saccharomyces/Silicon Ferment, Butylene Glycol, Glyceryl Acrylate/Acrylic Acid Copolymer, Glyceryl Caprylate, Sucrose Stearate, Lecithin, Polyglyceryl-10 Laurate, Xanthan Gum, Cetearyl Olivate, Sorbitan Olivate, Sodium Citrate, Carbomer, Citric Acid, Cetearyl Alcohol, Sodium Hydroxide, Lactobacillus Ferment, Leuconostoc/Radish Root Ferment Filtrate, Hydroxyacetophenone, Iron Oxides (CI 77491).*******Podcast LinksApple - https://b.link/No_BS_Apple_PodcastGoogle - https://b.link/No_BS_Google_PodcastAmazon - https://b.link/No_BS_Amazon_PodcastSpotify - https://b.link/No_BS_Spotify_PodcastStitcher - https://b.link/No_BS_Stitcher_PodcastRSS - https://feeds.redcircle.com/671dd1b2-a989-41d5-94d5-30c014e06149********Sephora - https://fxo.co/1231867/sephoraUlta - https://fxo.co/1231867/ultaAmazon - https://www.amazon.com/shop/nobsbeautyYes Style - https://ys.style/kk2Vjrv798Style Korean - http://www.stylekorean.com/?af_id2=nobsbeautyThese are affiliate links if you purchase anything from one of these stores using this link No BS Beauty will make a small commission on what you buy.********I am proud to offer my very own beauty products at Amazon. We are starting small but hope to grow these offerings. Take a look and if you can pick one or two up, it helps keep this channel truly independent.My Products:No BS Beauty Travel Set - https://amzn.to/2PgPzFZNo BS Beauty Airless Jars - https://bit.ly/2Ev6X6N or https://amzn.to/2RCEq4sNo BS Beauty Color Switcher - https://amzn.to/2RCEAJ6See my own page on Amazon - https://www.amazon.com/shop/nobsbeautywww.noBSbeauty.net*******My Patreon - https://www.patreon.com/noBSbeauty*******PayPal Tip Jar - https://bit.ly/donate_NBSBIf you want to leave a tip ... Thanks! *****Since so many of you asked for it, here is a link to my favorite PH testing strips https://amzn.to/33ojjIY

Phosphatidyl Serine (PS) can play a critical role in both maintaining and improving mental performance – name and face recognition, recalling locations of misplaced objects, concentration, and focus – which is often an unfortunate component of the normal aging process. A multitude of scientific studies have shown that SerinAid PS can significantly help maintain and improve mental performance, can help improve mood, and can make a valuable contribution to lowering both physical and psychological stress. FDA Approved Qualified Claim Recognizing how effective PS really is, the US Food and Drug Administration (FDA), in February, 2003, granted two significant health claims for PS, indicating that PS may reduce the risk of cognitive dysfunction and dementia in the elderly. The post 395 – Phosphatidyl Serine for Dementia and Cognitive Decline appeared first on Health Quest Podcast.

Serine Avetisyan is a former COAF Debate Monitoring Specialist who is now working for the Girls of Armenia Leadership Soccer, aka GOALS NGO. Serine shares insights on GOALS' initiatives in Armenia and Artsakh.Website: www.coaf.orgFacebook: https://www.facebook.com/coafkidsInstagram: https://www.instagram.com/coafkids/Twitter: https://twitter.com/coafkidsLinkedIn: https://www.linkedin.com/company/coafkids/ 

Drunk Elephant Protini Powerpeptide Resufacing Serum At Sephora: https://fxo.co/CaroFull Ingredients List:  Water/Aqua/Eau, Glycerin, Lactic Acid, Dicaprylyl Carbonate, Squalane, Sodium Hydroxide, Glycine Max (Soybean) Seed Extract, Sclerocarya Birrea Seed Oil, Camellia Sinesis Seed Oil, Sodium PCA, Caprylic/Capric Triglyceride, Borago Officinalis Seed Oil, Tremella Fuciformis Polysaccharide, Tocotrienols, Sh-Oligopeptide-1, Sh-Oligopeptide-2, Sh-Polypeptide-1, Sh-Polypeptide-9, Sh-Polypeptide-11, Copper Palmitoyl Heptapeptide-14, Heptapeptide-15 Palmitate, Palmitoyl Tetrapeptide-7, Palmitoyl Tripeptide-1, Palmitoyl Tripeptide-38, Palmitoyl Hexapeptide-12, Tripeptide-1, Pyrus Malus (Apple) Fruit Extract, Propanediol, Sodium Hyaluronate, Panthenol, Sodium Hyaluronate Crosspolymer, Sodium Lactate, PCA, Alanine, Arginine, Glycine, Histidine, Isoleucine, Phenylalanine, Proline, Serine, Threonine, Valine, Adenosine, Nymphaea Alba Root Extract, Bacillus/Folic Acid Ferment Filtrate Extract, Symphytum Officinale Callus Culture Extract, Dextran, Oryza Sativa (Rice) Bran Oil, Linoleic Acid, Linolenic Acid, Butylene Glycol, Acetyl Glutamine, Coconut Alkanes, Coco-Caprylate/Caprate, Pentylene Glycol, Isomalt, Lecithin, Xanthan Gum, Aspartic Acid, Phytosphingosine, Phospholipids, Glycine Soja (Soybean) Sterols, Tocopherol, Citric Acid, Lactic Acid/Glycolic Acid Copolymer, Polyvinyl Alcohol, Phenoxyethanol, Sodium Benzoate, Hydroxypropyl Cyclodextrin, Polysorbate 20, Caprylyl Glycol, Phenylpropanol, Chlorphenesin, Carbomer, Glyceryl Caprylate, Ethylhexylglycerin.Sunday Riley Good Genes All in One Lactic Acid TreatmentAt Sephora: https://fxo.co/CarpFull Ingredients List:  Botanical Blend [Aqua, Opuntia Tuna Fruit Extract, Cypripedium Pubescens Extract, Opuntia Vulgaris Leaf Extract, Agave Tequilana Leaf Extract, Arnica Montana Flower Extract, Aloe Barbadensis Leaf Extract, Saccharomyces Cerevisiae (Yeast) Extract, Leuconostoc/Radish Root Ferment Filtrate], Caprylic/Capric Triglyceride, Squalane, Lactic Acid, PPG-12 / SMDI Copolymer, Propanediol, Butylene Glycol, Stearic Acid, Dimethicone, Cetearyl Alcohol, Phenyl Trimethicone, Disiloxane, Ceteareth-20, Glyceryl Stearate, PEG-100 Stearate, Stearyl Glycyrrhetinate, Glycyrrhiza Glabra Root Extract, Cymbopogon Schoenanthus (Lemongrass) Oil, Potassium Hydroxide, Phenoxyethanol, Xanthan gum, Caprylyl Glycol, Chlorphenesin, Sodium Phytate.*******Podcast LinksApple - https://b.link/No_BS_Apple_PodcastGoogle - https://b.link/No_BS_Google_PodcastAmazon - https://b.link/No_BS_Amazon_PodcastSpotify - https://b.link/No_BS_Spotify_PodcastStitcher - https://b.link/No_BS_Stitcher_PodcastRSS - https://feeds.redcircle.com/671dd1b2-a989-41d5-94d5-30c014e06149********Sephora - https://fxo.co/1231867/sephoraUlta - https://fxo.co/1231867/ultaAmazon - https://www.amazon.com/shop/nobsbeautyYes Style - https://ys.style/kk2Vjrv798Style Korean - http://www.stylekorean.com/?af_id2=nobsbeautyThese are affiliate links if you purchase anything from one of these stores using this link No BS Beauty will make a small commission on what you buy.********I am proud to offer my very own beauty products at Amazon. We are starting small but hope to grow these offerings. Take a look and if you can pick one or two up, it helps keep this channel truly independent.My Products:No BS Beauty Travel Set - https://amzn.to/2PgPzFZNo BS Beauty Airless Jars - https://bit.ly/2Ev6X6N or https://amzn.to/2RCEq4sNo BS Beauty Color Switcher - https://amzn.to/2RCEAJ6See my own page on Amazon - https://www.amazon.com/shop/nobsbeautywww.noBSbeauty.net*******My Patreon - https://www.patreon.com/noBSbeauty*******PayPal Tip Jar - https://bit.ly/donate_NBSBIf you want to leave a tip ... Thanks! *****Since so many of you asked for it, here is a link to my favorite PH testing strips https://amzn.to/33ojjIY

Drunk Elephant Protin Resurf Serum at Sephora: https://fxo.co/CSydDrunk Elephant Protini Powerpeptide Resurfacing Serum Full Ingredients List:Water/Aqua/Eau, Glycerin, Lactic Acid, Dicaprylyl Carbonate, Squalane, Sodium Hydroxide, Glycine Max (Soybean) Seed Extract, Sclerocarya Birrea Seed Oil, Camellia Sinesis Seed Oil, Sodium PCA, Caprylic/Capric Triglyceride, Borago Officinalis Seed Oil, Tremella Fuciformis Polysaccharide, Tocotrienols, Sh-Oligopeptide-1, Sh-Oligopeptide-2, Sh-Polypeptide-1, Sh-Polypeptide-9, Sh-Polypeptide-11, Copper Palmitoyl Heptapeptide-14, Heptapeptide-15 Palmitate, Palmitoyl Tetrapeptide-7, Palmitoyl Tripeptide-1, Palmitoyl Tripeptide-38, Palmitoyl Hexapeptide-12, Tripeptide-1, Pyrus Malus (Apple) Fruit Extract, Propanediol, Sodium Hyaluronate, Panthenol, Sodium Hyaluronate Crosspolymer, Sodium Lactate, PCA, Alanine, Arginine, Glycine, Histidine, Isoleucine, Phenylalanine, Proline, Serine, Threonine, Valine, Adenosine, Nymphaea Alba Root Extract, Bacillus/Folic Acid Ferment Filtrate Extract, Symphytum Officinale Callus Culture Extract, Dextran, Oryza Sativa (Rice) Bran Oil, Linoleic Acid, Linolenic Acid, Butylene Glycol, Acetyl Glutamine, Coconut Alkanes, Coco-Caprylate/Caprate, Pentylene Glycol, Isomalt, Lecithin, Xanthan Gum, Aspartic Acid, Phytosphingosine, Phospholipids, Glycine Soja (Soybean) Sterols, Tocopherol, Citric Acid, Lactic Acid/Glycolic Acid Copolymer, Polyvinyl Alcohol, Phenoxyethanol, Sodium Benzoate, Hydroxypropyl Cyclodextrin, Polysorbate 20, Caprylyl Glycol, Phenylpropanol, Chlorphenesin, Carbomer, Glyceryl Caprylate, Ethylhexylglycerin.********Podcast LinksApple - https://b.link/No_BS_Apple_PodcastGoogle - https://b.link/No_BS_Google_PodcastAmazon - https://b.link/No_BS_Amazon_PodcastSpotify - https://b.link/No_BS_Spotify_PodcastStitcher - https://b.link/No_BS_Stitcher_PodcastRSS - https://feeds.redcircle.com/671dd1b2-a989-41d5-94d5-30c014e06149********Sephora - https://fxo.co/1231867/sephoraUlta - https://fxo.co/1231867/ultaAmazon - https://www.amazon.com/shop/nobsbeautyYes Style - https://ys.style/kk2Vjrv798Style Korean - http://www.stylekorean.com/?af_id2=nobsbeautyThese are affiliate links if you purchase anything from one of these stores using this link No BS Beauty will make a small commission on what you buy.********I am proud to offer my very own beauty products at Amazon. We are starting small but hope to grow these offerings. Take a look and if you can pick one or two up, it helps keep this channel truly independent.My Products:No BS Beauty Travel Set - https://amzn.to/2PgPzFZNo BS Beauty Airless Jars - https://bit.ly/2Ev6X6N or https://amzn.to/2RCEq4sNo BS Beauty Color Switcher - https://amzn.to/2RCEAJ6See my own page on Amazon - https://www.amazon.com/shop/nobsbeautywww.noBSbeauty.net*******My Patreon - https://www.patreon.com/noBSbeauty*******PayPal Tip Jar - https://bit.ly/donate_NBSBIf you want to leave a tip ... Thanks! *****Since so many of you asked for it, here is a link to my favorite PH testing strips https://amzn.to/33ojjIY

Kinderen opvoeden wordt kinderspel dankzij de nieuwe opvoedingstechnieken van Fien, Serine, Bert en special guest Jani Kazaltzis!

Serine Ayari is actrice en stand-upcomedian, die optreedt in het Engels, Frans én het Nederlands. Vorig jaar was Ayari ook te zien in De Slimste Mens. Maar daar hebben we het in deze aflevering amper over. In dit gesprek toont Serine haar kwetsbare kant en praat ze openhartig over haar ADHD, indruisen tegen de norm, emotionele rollercoasters en nog zoveel meer.

#VideoGames #Arcade #Asteroids #RetroAnd with those words, the story of Asteroids on the Atari 2600 began.  Mike never had an Atari back in the day, but he got to play this game on a cold, quiet serine evening in January 1980.  Want to know more about the history of this arcade port?  Watch and learn!5 min. Gaming With Mike on YouTube:  https://www.youtube.com/playlist?list=PLvke-gQ4wgZLVdfctzPZw7P-UERFmN6TEYouTube link to this show:  COMING SOON!If you like this video, please hit that Like button! Subscribe and hit that notification bell, as well! Follow us here:Email: TheWatchMenShow@gmail.comFacebook: https://www.facebook.com/TheWatchMenShow/Twitter: https://twitter.com/TheWatc63749470Thank you for spending your time with The Watch Men and Gaming With Mike For 5 Minutes!

Background music for Mindfulness and Consciousness purposes

Ça parle d'un énorme silence (10 secondes sur scène = 5 ans dans la vie) suite à la mauvaise interprétation d'une spectatrice... Retrouvez Serine Ayari : ⚫Facebookhttps://www.facebook.com/serineayaricomedy/ ⚫Instagramhttps://instagram.com/serine.ayari ⚫Twitterhttps://twitter.com/Serineayari_ ⚫YouTubehttps://youtube.com/channel/UCNT4Y_o3BgXU5EGZyv-ZuEw Et bien sûr vous pouvez également me retrouver sur les réseaux sociaux: ⚫Facebookhttps://www.facebook.com/SofianeHumoriste ⚫Instagramhttps://www.instagram.com/sofiane_ettai ⚫Twitterhttps://twitter.com/SofianeETTAI ⚫YouTubehttps://www.youtube.com/channel/UC6ZmnGo-1bsiK8t2CptHn1g

Question:  Do low serine levels require IV supplements?You're probably just depleting serine through the CBS pathway and just eat more protein and consider supplementing glycine or collagen along with it. I don't see why that would require IV supplements.  If you would like to be part of the next live Ask Me Anything About Nutrition, sign up for the CMJ Masterpass, which includes access to these live Zoom sessions, a private discussion group, premium features on all my content, and hundreds of dollars of exclusive discounts. You can sign up at https://chrismasterjohnphd.com/masterpass/ and use the code QANDA to get 10% off the membership for life. For the remainder of 2020, I will be working full-time on finishing my Vitamins and Minerals 101 book, while reserving a portion of my time for consulting clients. You can pre-order my book at https://chrismasterjohnphd.com/book. You can sign up for a consultation at https://chrismasterjohnphd.com/consultations  DISCLAIMER: I have a PhD in Nutritional Sciences and my expertise is in performing and evaluating nutritional research. I am not a medical doctor and nothing herein is medical advice.

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2020.10.28.358432v1?rss=1 Authors: Akram, S., Thakur, J., Shree, M., Masakapalli, S. K., Nanda, R. K. Abstract: In vitro studies involving cell lines or primary cells, provide critical insights into their physiology under normal and perturbed conditions like cancer and infection. Given that there are multiple sources of carbon, nitrogen, and other nutrients available in routinely used standard media (such as DMEM, RPMI), it is vital to quantify their contribution to cellular metabolism. 13C based Isotopic tracers of the media components can be used to kinetically track their oxidation by the cell systems such as Human Lung Carcinoma (A549) cells. In this study, a universally labelled glucose tracer ([13C6]glucose) was used to quantify its metabolic contribution that provided further insights into the central carbon metabolism of A549 cells. Gas chromatography and mass spectrometry (GC-MS) based mass isotopomer analysis (average 13C) of methanolic extracts (glycerol: 5.46{+/-}3.53 % and lactate: 74.4{+/-}2.65 %), amino acids derived from acid hydrolysates of protein (Serine: 4.51{+/-}0.21 %, Glycine: 2.44{+/-}0.31 %, Alanine: 24.56{+/-}0.59 %, Glutamate: 8.81{+/-}0.85 %, Proline: 6.96{+/-}0.53 % and Aspartate: 10.72{+/-}0.95 %) and the metabolites of the culture filtrate (glycerol: 43.14{+/-}1.45 % and lactate: 81.67{+/-}0.91 %), allowed to capture the relative contribution of glucose. We observed the Warburg effect and a significant amount of lactate contributed from glucose, was released to the media. 13C glycerol of glucogenic origin was kinetically released to the culture filtrate and might be playing a critical role in metabolic reprogramming of A549 cells. Part of the protein biomass contributed from amino acids were of glucogenic origin. Besides, the workflow adopted for 13C analysis and derived average 13C of each metabolite provided a standard methodology that could be useful in defining the metabolic phenotypes of cells in normal and perturbed conditions. Understanding precisely the altered cellular metabolism to meet the biomass demand under a range of physiological conditions, kinetically, may identify pathways for targeted and effective therapeutic interventions. Copy rights belong to original authors. Visit the link for more info

DE EERSTE TAJINE TALKS LIVE!Op het POINT OF U event in Antwerpen.We hebben het over de diversiteit in de media, samen met comedian Serine Ayari!Hoe zou het komen dat niet iedereen de weg vindt naar de media? Niet gerepresenteerd?Ergens binnenkomen en je meteen de vreemde eend in de bijt voelen?Herkennen steeds minder POC kijkers en makers zichzelf in het televisieaanbod?En hoe kunnen we dat oplossen?Enjoy, like swipe, press, push, rate, dance, en laat maar weten! Volgen kan en mag! via instagram of facebookVragen, opmerkingen of meningen? info@tajinetalks.beSocials:HakimmJolien RoetsSerine AyariPoint Of U Festival

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2020.09.18.304170v1?rss=1 Authors: Jami, S. A., Cameron, S., Wong, J. M., Daly, E. R., McAllister, A. K., Gray, J. A. Abstract: There is substantial evidence that both NMDA receptor (NMDAR) hypofunction and dysfunction of GABAergic neurotransmission contribute to schizophrenia, though the relationship between these pathophysiological processes remains largely unknown. While models using cell-type-specific genetic deletion of NMDARs have been informative, they display overly pronounced phenotypes extending beyond those of schizophrenia. Here, we used the serine racemase knockout (SRKO) mice, a model of reduced NMDAR activity rather than complete receptor elimination, to examine the link between NMDAR hypofunction and decreased GABAergic inhibition. The SRKO mice, in which there is a >90% reduction in the NMDAR co-agonist D-serine, exhibit many of the neurochemical and behavioral abnormalities observed in schizophrenia. We found a significant reduction in inhibitory synapses onto CA1 pyramidal neurons in the SRKO mice. This reduction increases the excitation/inhibition balance resulting in enhanced synaptically-driven neuronal excitability and elevated broad-spectrum oscillatory activity in ex vivo hippocampal slices. Consistently, significant reductions in inhibitory synapse density in CA1 were observed by immunohistochemistry. We further show, using a single-neuron genetic deletion approach, that the loss of GABAergic synapses onto pyramidal neurons observed in the SRKO mice is driven in a cell-autonomous manner following the deletion of SR in individual CA1 pyramidal cells. These results support a model whereby NMDAR hypofunction in pyramidal cells disrupts GABAergic synapse development leading to disrupted feedback inhibition and impaired neuronal synchrony. Copy rights belong to original authors. Visit the link for more info

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2020.08.18.255380v1?rss=1 Authors: Vongsouthi, V., Whitfield, J. H., Unichenko, P., Mitchell, J. A., Breithausen, B., Khersonsky, O., Kremers, L., Monai, H., Hirase, H., Fleishman, S. J., Henneberger, C., Jackson, C. J. Abstract: Periplasmic solute-binding proteins (SBPs) have evolved to balance the demands of ligand affinity, thermostability and conformational change to carry out diverse functions in small molecule transport, sensing and chemotaxis. Although the ligand-induced conformational changes that occur in SBPs make them useful components in biosensors, their complexity can be difficult to emulate and they are challenging targets for protein engineering and design. Here we have engineered a fluorescent biosensor with specificity for the signalling molecule D-serine (D-SerFS) from a D-alanine-specific SBP. Through a combination of binding site and remote mutations, the affinity, specificity and thermostability were optimized to allow the detection of changes in D-serine levels using two-photon excitation fluorescence microscopy in situ and in vivo. This work illustrates the multidimensional constraints that are imposed by the trade-offs between structural dynamics, ligand affinity and thermostability, and how these must be balanced to achieve desirable activities in the engineering of complex, dynamic proteins. Copy rights belong to original authors. Visit the link for more info

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2020.08.11.244939v1?rss=1 Authors: Raskar, T., Niebling, S., Devos, J. M., Yorke, B. A., Härtlein, M., Huse, N., Forsyth, T. V., Seydel, T., Pearson, A. R. Abstract: Incoherent neutron spectroscopy, in combination with dynamic light scattering was used to investigate the effect of ligand binding on the center-of-mass self-diffusion and internal diffusive dynamics of E.coli aspartate -decarboxylase (ADC). The X-ray crystal structure of the D-serine inhibitor complex with ADC was also determined, and molecular dynamics simulations used to further probe the structural rearrangements that occur as a result of ligand binding. These experiments reveal the existence of higher order oligomers of the ADC tetramer on ns-ms time-scales, and also show that ligand binding both affects the ADC internal diffusive dynamics and appears to further increase the size of the higher order oligomers. Copy rights belong to original authors. Visit the link for more info

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2020.08.10.244822v1?rss=1 Authors: Wiriyasermkul, P., Moriyama, S., Tanaka, Y., Kongpracha, P., Nakamae, N., Suzuki, M., Kimura, T., Mita, M., Sasabe, J., Nagamori, S. Abstract: Delay in diagnosis of renal injury has profound impacts on morbidity and mortality. Damage of proximal tubules by the injury impairs not only individual membrane transport proteins but also coordinated transport systems. In this study, we analyzed the proteome of apical membrane of proximal tubular epithelium from the mouse kidney with early ischemiareperfusion injury (IRI), a well-known acute kidney injury (AKI) model. The proteome showed drastic elevations of the injury-responsive proteins and depressions of abundant transporters, leading to the prediction of biomarkers for early IRI. As the benchmark of our in-depth analysis from the proteome, we characterized transporters for D-serine, a promising renal diseases' biomarker. Using cell-based and cell-free methods, we identified sodium-coupled monocarboxylate transporters (SMCTs) as the D-serine transporters. This finding enlightens the role of non-canonical substrate transport and clarifies the D-serine transport systems in the kidney. Our approaches can be applied for investigation of other membrane transport systems. Copy rights belong to original authors. Visit the link for more info

In this session Dr Guerra discusses the physiological and pathophysiological roles of serine protease inhibitors (SERPINS). For example, the physiological role of plasminogen activator inhibitor 1 (PAI-1) involves control over fibrinolysis which is an essential hemostatic mechanism for regulating the dissolution of fibrin clots associated with airway passage remodeling systemic wound repair, and the inflammatory response as linked to cytokine mediated immune responses and circulating t-PA mediated cardiovascular function. Dr Guerra also mentioned that he has established a PATREON site: https://www.patreon.com/user?u=37615512&fan_landing=true&fbclid=IwAR0L8ADIhtIgZM_WtkJejz_x-3hAdzr39mrvoZxdAu6glm9zkAG5L4NnhCo Lease strongly consider recombining with the Patreon link as a means to provide monetary donations to the Authentic Biochemistry Podcast and YouTube lecture series plus the VerEvMed Facebook postings that include Authentic Biochemistry "Minutes" and some original (North)Western philosophy, courtesy of Dr Guerra and his existentialist individualism. --- Support this podcast: https://anchor.fm/dr-daniel-j-guerra/support

Serine comes back to the podcast for a special Black Lives Matter-focused series of episodes during this movement. It's a serious talk about the perspective of an ally during this momentous civil rights surge and how best to support & amplify voices of the movement. Music by KarpaBeats

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2020.06.16.155572v1?rss=1 Authors: Wong, J. M., Folorunso, O., Barragan, E. V., Berciu, C., Harvey, T. L., DeChellis-Marks, M. R., Glausier, J. R., MacDonald, M. L., Coyle, J. T., Balu, D. T., Gray, J. A. Abstract: D-serine is the primary NMDA receptor (NMDAR) co-agonist at mature forebrain synapses and is synthesized by the enzyme serine racemase (SR). However, our understanding of the mechanisms regulating the availability of synaptic D-serine remains limited. Though early studies suggested D-serine is synthesized and released from astrocytes, more recent studies have demonstrated a predominantly neuronal localization of SR. More specifically, recent work intriguingly suggests that SR may be found at the postsynaptic density, yet the functional implications of postsynaptic SR on synaptic transmission are not yet known. Here, we show an age-dependent dendritic and postsynaptic localization of SR and D-serine by immunohistochemistry and electron microscopy in mouse CA1 pyramidal neurons, as well as the presence of SR in human hippocampal synaptosomes. In addition, using a single-neuron genetic approach in SR conditional knockout mice, we demonstrate a cell-autonomous role for SR in regulating synaptic NMDAR function at Schaffer collateral (CA3)-CA1 synapses. Importantly, single-neuron genetic deletion of SR resulted in the elimination of LTP at one month of age. Interestingly, there was a restoration of LTP by two months of age that was associated with an upregulation of synaptic GluN2B. Our findings support a cell-autonomous role for postsynaptic neuronal SR in regulating synaptic NMDAR function and suggests a possible autocrine mode of D-serine action. Copy rights belong to original authors. Visit the link for more info

Godswill gets serious for once with his friend Serine to talk about Muslims fasting the month of Ramadan! Godswill with his Christian perspective takes the time to ask questions about Islam and to reflect on his experience with fasting, especially now during the shelter-in-place conditions. Check out this enlightening episode now. Music by KarpaBeats

As we continue to explore how this period fosters (or hinders) creativity, Serine Ayari came on as a guest for this episode. She is a multilingual comedian/actress based in Belgium. We discussed her ability to write jokes for audiences with different sensibilities, the origins of her comedy and how she deals with political correctness. The future is female, so mark your calendar to attend her upcoming one woman show "Still Standing"!Listen & share, it's the Brooklyn way!

We have shown love to Serine Karthage for a long time on SISTER and we finally got her in to the studio to talk with Carly Wilford about her recent musical journey and plans for future releases...

Ready for a refresher on why micronutrients matter? Wonder if nutrient deficiency could be at the root of your health concerns? Tune in to hear Ali and Becki discuss folate, serine and niacin as 3 micronutrients that matter! Learn about their function, symptoms of deficiency as well as food sources of these important nutrients and when you should turn to supplementation for repletion.    In this episode, Ali and Becki break down the reasons stress can deplete micronutrients, especially B vitamins like folate and niacin and those used in regulation of cortisol like serine. Learn about symptoms and disease states associated with deficiency of these nutrients and when and why you want to consider supplementation. Plus, hear our take on the importance of avoiding synthetic folate, whether NAD+ is a miracle supplement and why you need to be including organ meats and grassfed whey in your diet!    Also in This Episode: Past Episodes on MicronutrientsEpisode 5: 3 Micronutrients You Need in Your Diet and Why Episode 72: 3 Micronutrients: Magnesium, Vitamin C and Glutamine Episode 85: 3 Micronutrients: Asparagine, CoQ10 and Zinc Episode 102: 3 Micronutrients: Pantothenate, Choline and Selenium Why Nutrient Deficiency OccursEpisode 69: Epigenetics and Methylation Episode 70: Dirty Genes with Dr. Ben Lynch Episode 136: Prescription Drivers of Body Imbalance How Stress Impacts Micronutrient StatusThe Anti-Anxiety Diet Micronutrient Panel FolateFunction Symptoms of Deficiency Food SourcesLiver Pills Why Watch Out for Folic Acid?Unenriched Nutritional Yeast How to Supplement with FolateMultiAvail Mama Multidefense with Iron Multidefense without Iron B Complex Blisphora: Sam-e, Methylated B-12 and Folate Why Calm and Clear is Folate Free SerineSymptoms of Deficiency Food Sources of SerineNaturally Nourished Grassfed Whey Supplement Strategy for SerineCalm and Clear NiacinFunction of Niacin in the Body Impact on Cardiovascular Health and Lipid Panels Niacin Supplementation500mg CRT Niacin - Designs for Health NAD+, Metabolic Miracle?   Sponsors for This Episode: This episode is sponsored by Wild Foods, a company that puts quality, sustainability, and health first in all of their products. They have everything from coffee to turmeric to medicinal mushrooms, and every single product is painstakingly sourced from small farms around the globe. They take their mission seriously to fix the broken food system, and believe real food is medicine. They've partnered with us to give you guys an exclusive discount, so use the code ALIMILLERRD for 12% off your order at WildFoods.co!    This episode is also sponsored by FBomb, makers of high-quality fat-fueled snacks and products like macadamia nut butter packs and premium oils as well as their new Keto Krunch and Pork Sticks. Click here for more on why I love FBomb and use code ALIMILLERRD for 10% off your order!  

Serine Ayari is een Vilvoordse die scoort in het buitenland! Ze is nog niet zo lang bezig als stand-up comedian maar speelde al in Frankrijk en New York, en binnenkort is er de premiere van haar eerste zaalshow, in de Arenberg in Antwerpen.

Dr Carolyn Lam:                Welcome to Circulation on the Run, your weekly podcast summary and backstage pass to the Journal and its editors. We're your cohosts. I'm Dr Carolyn Lam, Associate Editor from the National Heart Center and Duke National University of Singapore. Dr Greg Hundley:             I'm Greg Hundley, Associate Editor of Circulation, Director of the Pauley Heart Center at VCU Health in Richmond, Virginia. Dr Carolyn Lam:                Greg, guess what? We are going to be talking later about non-inferiority trials. Now, you're going to go like, "Huh? What?," but then we see more and more non-inferiority cardiovascular trials. And do we really know the advantages and limitations of this type of trial design? Which is so important to understand, because we need to understand the factors that may impact our confidence and interpretation of these results. So, that's going to be a really important feature discussion, coming up right after our coffee chat. Greg, what are your papers? Dr Greg Hundley:             Thanks Carolyn. Boy, I can't really wait to get to that feature discussion. That's something that we deal with all the time, and I look forward to that explanation and that discussion. I'm going to talk a little bit of basic science, with two papers right in a row.                                                 And the first one involves catecholaminergic polymorphic ventricular tachycardia through inhibition of calcium/calmodulin-dependent kinase II. The lead author is Dr Vassilios Bezzerides from Boston Children's Hospital.                                                 Carolyn, this paper focuses on treatment of catecholaminergic polymorphic ventricular tachycardia, an underlying diagnosis in at least 12% of pediatric patients who present with unheralded cardiac arrest. ICDs, as you know, are frequently implanted, but are problematic because of increased complication rates in pediatric patients, failure to convert ventricular arrhythmias, and the risk of fatal ICD-induced electrical storm. Modulating CaM Kinase II within the heart shows promise to treat this, but CaM Kinase II is essential in other tissues, most notably the brain. Dr Carolyn Lam:                How interesting. So, what did the study show? Dr Greg Hundley:             Well, the investigator used adeno-associated viral gene therapy, which is proven to be a safe and efficient vector for sustained gene transfer into many cell types to selectively inhibit CaM Kinase II in cardiomyocytes. They were able to express the specific CaM kinase II inhibitory peptide AIP in cardiomyocytes without significant extra cardiac expression, and an inhibition of CaM Kinase II effectively suppressed ventricular arrhythmias in a murine model of catecholaminergic polymorphic ventricular tachycardia after a single therapeutic dose. So thus, in animal models, delivery of a CaM Kinase inhibitory peptide by AAV represents a novel single dose gene therapy for catecholaminergic polymorphic ventricular tachycardia. How about that? Dr Carolyn Lam:                Wow. You've got a second paper? Dr Greg Hundley:             I sure do. So now we're going to jump into, again, looking at polymorphic VT from engineered human heart tissue. And this article is from Kevin Parker at Harvard University, "Modeling of Human Arrhythmias Using Induced Pluripotent Stem Cell-Derived Cardiomyocytes has Focused on Single-Cell Phenotypes."                                                 With this said, it is important to realize that arrhythmias are emergent properties of cells assembled into tissues and the impact of inherited Arrhythmia mutations on tissue level properties of human heart tissue. And therefore, newer technologies are needed to develop more satisfactory therapeutic interventions. Ones that encompass all of the tissue, not just single cells. Dr Carolyn Lam:                Interesting. So, what did this particular study do? Dr Greg Hundley:             So, Carolyn, in this study, the investigators report on an optogenetically-based human-engineered tissue model of catecholaminergic polymorphic VT, which as we have discussed in the previous article is promoted by mutation of the cardiac ryanodine channel 2, which is promoted by mutation of cardiac ryanodine channel and triggered by exercise. They developed a human IPSC cardiomyocyte-based platform to study the tissue level properties and investigated pathogenic mechanisms in polymorphic VT by combining this novel platform with genome editing. The authors found that engineered heart tissue, fabricated from human pluripotent stem cell derived cardiomyocytes, effectively modeled catecholaminergic polymorphic VT caused by dominant mutations in the cardiac ryanodine receptor, including induction of arrhythmias by conditions that stimulate exercise. Using selective pharmacology and genome editing, the authors identified activation of calcium/calmodulin-dependent kinase II, or CaM Kinase II, and CaM Kinase II mediated phosphorylation of ryanodine at Serine 2814 as critical events that are required to unmask the latent arrhythmic potential of catecholaminergic polymorphic VT, causing ryanodine mutations, highlighting a molecular pathway that links beta adrenergic stimulation to arrhythmogenesis in this disease. Dr Carolyn Lam:                Wow Greg! So, two very interesting and important linked genetic papers. Well, we're going to switch tracks a little bit and talk about, well, my favorite topic: heart failure with preserved ejection fraction and the whole complex issue of the diagnosis of this syndrome. Now we know that the diagnosis is kind of complex and there is currently no consensus but several proposed definitions. So how do the clinical and hemodynamic profile of patients vary across the different definitions of HFpEF?                                                 So, this question was answered by Dr Jennifer Ho from Massachusetts General Hospital and her colleagues, who examined consecutive patients with chronic exertional dyspnea and an ejection fraction above 50% who are referred for comprehensive cardiopulmonary exercise testing with invasive hemodynamic monitoring. They applied societal and clinical trial HFpEF definitions and compared the clinical profiles, exercise responses, and cardiovascular outcomes by these different definitions. So, of 461 patients, 416 met the ACC/AHA definition, 205 met the ESC definition, and 55 met the HFSA criteria for HFpEF.                                                 The clinical profiles and exercise capacity varied vastly across the definitions, with peak oxygen uptake averaging 16.2 for those with the ACC/AHA definition and down to 12.7 in those satisfying the HFSA definition. Dr Greg Hundley:             Wow. What a difference from these societies. Dr Carolyn Lam:                Mm-hmm (affirmative). Dr Greg Hundley:             So Caroline, it sounds like they looked at all comers with exertional dyspnea. Now how about those that had hemodynamic evidence of heart failure with preserved ejection fraction? Dr Carolyn Lam:                Yeah, good question Greg. So, you caught me telling you that all these patients had hemodynamic cats as well, and a total of 243 had hemodynamic evidence of HFpEF, which was defined as an abnormal rest or exercise feeling pressure. Of these, 222 met the ACC/AHA criteria, 161 met the ESC criteria, and only 41 met the HFSA criteria. Over a mean follow-up of 3.8 years, the incidents of cardiovascular outcomes range from 75 for the ACC/AHA criteria to 298 events per thousand-person years for the HFSA criteria.                                                 The application of clinical trial definitions of HFpEF similarly resulted in distinct patient classification and prognostication. So in summary, the authors demonstrated significant diversity in the number of patients meeting HFpEF criteria. And using different HFpEF classifications variably enriched for future cardiovascular events, but at the expense of not including up to 85% of individuals with physiologic evidence of HFpEF. Comprehensive phenotyping of patients with suspected heart failure really highlighted the limitations and heterogeneity of current HFpEF definitions and may help to refine HFpEF sub-grouping to test therapeutic interventions. Now, these are all discussed in an important accompanying editorial by Michele Senni, Sergio Caravita, and Walter Paulus. Dr Greg Hundley:             Wow Carolyn. It appears, depending upon the definition, we could really classify patients drastically differently. Dr Carolyn Lam:                Yeah, an important paper indeed. And again, I would strongly encourage everyone to read that editorial as well. For my second pick, we're going to switch to CPR in children.                                                 So these authors, now led by Dr Rohan Khera from UT Southwestern, examined the prevalence and predictors of survival of children who progress from bradycardia to pulselessness in in-hospital cardiac arrest despite cardiopulmonary resuscitation. So they looked at almost 5,600 pediatric patients age more than 30 days to under 18 years of age, who received CPR at hospitals participating in the Get With The Guidelines - Resuscitation during 2000 to 2016 each CPR event was classified as bradycardia with pulse, bradycardia with subsequent pulselessness, and initial pulseless cardiac arrest. And the authors assessed for risk adjusted rates of survival to hospital discharge. Dr Greg Hundley:             So Carolyn, what did they find? This is really interesting. Dr Carolyn Lam:                Well, among hospitalized children in whom CPR was initiated, half had bradycardia with poor perfusion at the initiation of chest compressions and nearly one third of these progressed to pulseless in-hospital cardiac arrest despite CPR. Survival was significantly lower for children who progressed to pulselessness despite CPR compared to those who were initially pulseless. So, these findings suggest that pediatric patients who lose their pulse despite CPR are at particularly high risk and require a renewed focus on post resuscitation care. Dr Greg Hundley:             Very interesting, Carolyn. Dr Carolyn Lam:                Well, that wraps it up for our discussion. Let's go onto the featured discussion. Shall we, Greg? Dr Greg Hundley:             You bet. Dr Carolyn Lam:                Non-inferiority cardiovascular trials are increasingly being published and in the highest impact journals. Yet how much do we really know about these designs of the trial, of the non-inferiority trials? Well, I have to admit not much in my point of view, and I was so pleased to see our feature discussion paper really published in this week's journal, which really digs deep into non-inferiority trials and talks about time trends and perhaps some lessons that we should all bear in mind when we look at these. I'm so pleased to have the first author, Dr Behnood Bikdeli, to tell us about the study. And he is from Columbia University Medical Center, New York Presbyterian Hospital, Yale Center of Outcomes, Research and Evaluation Core, as well as the Cardiovascular Research Foundation in New York. We also have Dr Naveed Sattar, associate editor from the University of Glasgow. Behnood, could you tell us, so what made you look at this question and what did you find? Dr Behnood Bikdeli:        For a while we've been very interested in profiling the cardiovascular trials, trying to understand a little better, what are the specific characteristics of the major child that we rely upon for research but also for clinical practice? Years ago, we did some studies for surrogate outcome trials and this, let's just say subsequent piece, where we tried to look into a randomized cardiovascular trial that use a non-inferiority design. We had a series of features in terms of quality metrics and methodological metrics that we wanted to look into. The over eight almost 27-year period, we identified 111 of these trials. Reassuringly, most of these trials inherited several important quality and methodological metrics that we were looking into. However, we also saw a significant room for improvement. There were quite a few quality or methodological metrics that some of these trials were not adhering to and we think it's important because ultimately for the design reporting and last reading of these trials, knowing these pluses and minuses would help inform people. Dr Carolyn Lam:                That's great, Behnood. Now for those of us listening who don't think about this every day, could you give us some examples of the top errors perhaps to look out for? Dr Behnood Bikdeli:        For example, in the typical superiority trials, when we want to test an intervention a versus intervention B, all that matters is we do a very good and adequately sized trial and rest of it is up to the study and how it goes to see whether or not something panned out. There was a significant difference between the new intervention versus the older ones, but in non-inferiority trials we have something called the non-inferiority margin and that's very highly relevant when it comes to the outcome that you're assessing when it comes to the ultimate results of the trial.                                                 If the investigators choose a very lenient y non-inferiority margin, they may end up calling an intervention non-inferior. They may give it a pass. While in reality the intervention has quite a lot of a risk or harmful profile compared with standard of care. But in the other side, as a clinical example, we have several interventional tools at hand, like transcatheter aortic valve replacement. Most of the indications where it's currently used came from large bell designed non-inferiority trials. Where they showed that it was almost as good as surgery, in some cases better, but also it had a lot of ancillary advantages. Dr Carolyn Lam:                Thanks, Behnood. And you know here, I just want to call out to the readers. You have to look at this paper. Look at the tables and figures which are really so helpful. And Naveed, can I bring you in on this now? I mean, I just love this paper. It's such an important topic and I've never seen it addressed like this before. Could you tell us a little bit about what the editors discussed when we looked at this? Dr Naveed Sattar:            I've been involved in a few non-inferiority trials and some of the factors that many of us discuss and some of course associated, sort of our clinical trials, and I've been involved normally in superiority trials, but increasingly we have cut our teeth in non-inferiority trials. So, some of the points that the paper picks up resonated well with us in terms of, one of the examples was Behnood and his team found that around about 40% of trials didn't even justify what their non-inferiority margin was. And that's something I've actually had detailed discussions involved in various trials with. And that's a really important point, but it isn't a, you have to be able to justify why you choose particular margin and what that margin would mean to the community. Otherwise you potentially could just pick something out there which really doesn't allow you to make a really strong non-inferiority claim. And I think that's one of the factors that you found, Behnood. Is that correct? Behnood Bikdeli:              Absolutely. And that's a great point. Thank you. To us, there were two things about it. One was whether or not they provided any detailed justification for it exactly as you said, not that they're just picking up something because that's the sample size that they could achieve or that's the number that they felt comfortable with. But also the second piece of it, a respective of how they calculated or came up to the number, their readers have a right to know how this was calculated or were this came from, so it's the reporting part of it. Sometimes they reported both in the published paper and a study protocol or a design paper. Sometimes it was only in one of them. Sometimes as you mentioned, it was not mentioned in either, which puts the reader in a very difficult situation.                                                 So we think, and these were the best of the best trials in the highest impact journals. Probably if we look high and low elsewhere it's going to be even more challenging. So, we think there's a lot of room for improvement for the readers to expect cleaner, more comprehensive papers to come, but also for the trialist to report them with more clarity. Dr Naveed Sattar:            And going forward, issue a nice figure that shows that the trend is that we are going to see more of these trials probably because you've got lots of better treatments now. So, you know it's getting harder, in the sense, in many areas of cardiovascular medicine to show superiority. So, there is a need for more trials which actually show benefits beyond just perhaps the main outcome in ways that you've explained in the particular paper.                                                 Do you think the FDA does enough in this particular area in terms of this helping investigators decide what the non-inferiority margins are? Or is that something in terms of the quality of the trials that needs a bit more investigation? I think your papers partly are pushed to say, "Actually we need to do these better. We need to justify them better. We need to look at them better." Because actually they do have a greater influence going forward. Dr Behnood Bikdeli:        First, I cannot agree more. We are going to see a lot more of non-inferiority trials sort of, maybe because we have reached a ceiling effect when traditional intervention for superiority, but there's a lot of room to find interventions that are at least as safe or as good but have a lot of side advantages and ancillary benefits that's happened with some of the anticoagulants among other therapies available.                                                 In terms of the regulatory aspects, one of the things we were fortunate about was within our team, we had people with expertise on the trialist side while communicating with the regulatory bodies and also from people who were consulting to the FDA for assessment of non-inferiority trials. So, we were fortunate to look into several of the methodological or quality metrics that were being thought of and we consulted with the in-source guidelines and FDA guidelines. That said, I completely agree that, for example, the suggestions that you provided in one of the tables could hopefully help shape some of these trials in a more rigorous way. Or at least a reporting, which is also an important piece, would be more transparent ultimately for the readership. Dr Naveed Sattar:            Absolutely. And transparency is really pivotal so that the readers completely understand what was done, what was predefined, and what was found so that they can make a proper judgment. And probably the final question I have in terms of, you make a good point that actually if the trials are not done well and there's a bit of slippage either in terms of loss of data or methodological issues, that then really pushes a trial towards a "no", in a sense you get a false reassurance of non-inferiority, but partly because the methodology wasn't robust enough. And it's really very critical for these trials, perhaps at least as critical as they are in superiority trials, but perhaps even more so. Is that a fair judgment? Dr Behnood Bikdeli:        No, no, no. You're absolutely right. That's another very important point in the typical superiority trial, if any bias drives the results toward no difference. Investigators are naturally guarding against that because it's going to be very problematic in non-inferiority trials. Depending on the effect measure that they choose, it could actually falsely favor the intervention of interest because it might show a false assessment of non-inferiority, and there are ways to work around it. There are ways to correct for it, such as choosing both absolute and relative effect measures, which practically addresses this concern. Again, gets back to the importance of appropriate design and appropriate transparency to report the results in a robust way, both intentions to treat and has treated or per protocol, both relative effect measures and absolute effect measures. Dr Naveed Sattar:            My sense of and getting back to you, but I think this will be a really seminal paper for the community to look at and really help us as a community to improve our conduct of such trials in the future because there will be more of these coming forward. Dr Carolyn Lam:                And I couldn't have said that better, Naveed. I think the take home message is right there. Pick it up, have a look and especially have a look at those tables and figures. It's really going to help you read many, many journals.                                                 Thank you so much, Naveed and Behnood. Thank you audience for joining us on Circulation on the Run. Talk to you next week.                                                 This program is copyright American Heart Association 2019.  

Serine proteinases and their peptide inhibitors, the SERPINS, have a role to play in liver pathology and disease potentiation leading to subcellular protein aggregation, enhanced inflammation and ultimate hepatocellular carcinoma. Dr. Guerra of Authentic Biochemistry introduces this new plank in the knowledge platform being constructed to apprehend either the necessary or sufficient conditions in HCC. --- Support this podcast: https://anchor.fm/dr-daniel-j-guerra/support

BMAA is a toxin that may cause some forms of sporadic ALS. Here we discuss how L-serine, an inexpensive, widely available amino acid supplement, might block BMAA toxicity and thus be of use for preventing or slowing ALS progression.

BMAA is a toxin that may cause some forms of sporadic ALS. Here we discuss how L-serine, an inexpensive, widely available amino acid supplement, might block BMAA toxicity and thus be of use for preventing or slowing ALS progression.

Commentary by Dr. Valentin Fuster

This podcast leads you into the lives of two Muslim American women. They’re smart, funny, and huge dorks. Merium is an accountant and Serine is an economic consultant, both slowly figuring out the business world. While trying to navigate through life using an Islamic moral compass, they come across obstacles that force them to reevaluate their understanding of religion, society, and culture. Follow us on Twitter! @Sawtmarathawra Merium @imalittoteapot Serine @Sinseriously

http://radioen.net/podcast/Utvalgte/Anne-Jorunn%2016.11.13%20Jonathan%20Sena%20og%20Ida%20Serine%20Aanensen.mp3

Phosphatidyl Serine (PS) can play a critical role in both maintaining and improving mental performance - name and face recognition, recalling locations of misplaced objects, concentration, and focus. A multitude of scientific studies have shown that SerinAid PS can significantly help maintain and improve mental performance. The post 104 – Phosphatidyl Serine for Dementia and Cognitive Decline – My Interview with Scott Hagerman appeared first on Health Quest Podcast.

Serine proteases in cytoplasmic granules of neutrophils (NSPs), namely neutrophil elastase, cathepsin G (CG) and proteinase 3, have been under intense investigation for several decades. They are mainly known for their role in intracellular killing of pathogens and are also increasingly recognized as key regulators of innate immune responses. In 2009, I identified a fourth serine protease in neutrophils that has been completely overlooked and neglected so far. The aim of this thesis was an in-depth biochemical and functional characterization of this novel serine protease 4 (NSP4) of human neutrophils. Using monoclonal antibodies to NSP4, the distribution of NSP4 in normal human tissues was studied. NSP4 was observed only in neutrophils and neutrophil precursors of the bone marrow. The content of NSP4 in neutrophil lysates was about 20-fold lower compared to CG. Nevertheless, NSP4 was found to be released into the supernatant upon neutrophil activation. NSP4 could be further identified as a novel azurophil granule protein of neutrophils by Western blot analyses of subcellular fractions. For the functional analysis, the production and yield of recombinant NSP4 was clearly improved using different expression systems and DNA construct modifications. The proteolytic specificity was analyzed using E. coli peptide libraries, mass spectrometry and several synthetic peptide libraries. All these analyses clearly revealed an arginine specificity for NSP4. Consistent with this, NSP4 was strongly inhibited by heparin-accelerated antithrombin and C1 inhibitor and, with lower efficacy, by α1-proteinase inhibitor (α1PI). The data allowed me to generate an NSP4-specific α1PI variant that was shown to form covalent complexes with all NSP4 of neutrophil lysates and supernatants of activated neutrophils. This finding strongly indicated that NSP4 is fully processed and stored as an already activated enzyme in azurophil granules. In addition, dipeptidyl peptidase I (DPPI) was identified as the activator of NSP4 in vivo, as DPPI deficiency resulted in complete absence of NSP4 in a Papillon-Lefèvre patient. Analysis of cell-based calcium assays revealed that proteinase-activated receptor-2 may represent a potential natural substrate of NSP4. So far, NSP4-deficient mice did not show an abnormal phenotype under clean housing conditions. Activation of isolated neutrophils by phorbol esters or immune complexes was also not impaired. This study establishes NSP4 as the only arginine-specific pre-activated serine protease stored in azurophil granules of neutrophils that may fullfil a quite distinct, supportive role in neutrophil responses to tissue damage and bacterial infections.

Serine-threonine phosphorylation events regulate protein-protein interactions downstream of T cell receptor activation.

Wed, 7 Jan 2009 12:00:00 +0100 https://edoc.ub.uni-muenchen.de/9518/ https://edoc.ub.uni-muenchen.de/9518/1/Kessenbrock_Kai.pdf Kessenbrock, Kai ddc:570, ddc:500, Fakultä

Tue, 4 Dec 2007 12:00:00 +0100 https://edoc.ub.uni-muenchen.de/7819/ https://edoc.ub.uni-muenchen.de/7819/1/Fellows_Edward.pdf Fellows, Edward ddc:500, ddc:570, Fakultät für Biologie

Wed, 23 May 2007 12:00:00 +0100 https://edoc.ub.uni-muenchen.de/6930/ https://edoc.ub.uni-muenchen.de/6930/1/Zenner_Gerhardt.pdf Zenner, Gerhardt ddc:500, ddc:570, Fakul

The transcription factor C/EBPa regulates early steps of normal granulocyte differentiation since mice with a disruption of the C/EBPa gene do not express detectable levels of the G-CSF receptor and produce no neutrophils. We have recently shown that C/EBPa function is also impaired in acute myeloid leukemias. However, how the transcriptional activity of C/EBPa is regulated both in myelopoiesis and leukemogenesis, is not fully understood. The current study demonstrates that activated Ras enhances the ability of C/EBPa to transactivate the G-CSF receptor promoter and a minimal promoter containing only C/EBP DNA binding sites. Ras signaling activates C/EBPa via the transactivation domain, because it enhances the transactivation function of a fusion protein containing a Gal4 DNA binding domain and the C/EBPa transactivation domain, and does not change C/EBPa DNA binding. Ras acts on serine 248 of the C/EBPa transactivation domain, as it does not enhance the transactivation function of a C/EBPa serine 248 to alanine point mutant. Interestingly, serine 248 of C/EBPa is a PKC consensus site, and a PKC inhibitor blocks the activation of C/EBPa by Ras. Ras signaling phosphorylates C/EBPa on serine 248 in vivo. Finally, mutation of serine 248 to alanine obviates the ability of C/EBPa to induce granulocytic differentiation. These data suggest a model where Ras signaling enhances the activity of C/EBPa to induce granulocytic differentiation by phosphorylation of serine 248.

We have isolated and characterized cDNAs from Hydra which encode antistasin, a potent inhibitor of factor Xa in the vertebrate blood clotting cascade. Hydra antistasin is expressed in gland cells and represents a major class of transcripts from Hydra's head. Sequence analysis revealed that Hydra antistasin contains 6 internal repeats of a 25–26 amino acid sequence with a highly conserved pattern of 6 cysteine and 2 glycine residues identical to that in leech antistasin. Conservation of antistasin in a lower metazoan provides a potential link between the vertebrate and invertebrate coagulation systems.