Podcasts about Guanine

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2023.06.19.545304v1?rss=1 Authors: Heydasch, M., Hinderling, L., van Unen, J., Dobrzynski, M., Pertz, O. Abstract: Tightly regulated spatio-temporal Rho GTPase activity patterns regulate morphogenetic processes such as cell migration. Emerging evidence suggests that binding of Guanine nucleotide exchange factors (GEFs) and GTPase activating proteins (GAPs) to the cytoskeleton or adhesions mediate feedback regulation to spatio-temporal Rho GTPase activation. To explore such feedback regulation, we study the Rho specific GAP Deleted in Liver Cancer 1 (DLC1) which binds to focal adhesions (FAs) through mechanosensitive interactions. Using a FRET biosensor, we show that DLC1 loss of function leads to global increase in Rho activity and contractility throughout the cell without affecting a striking lamellar RhoA activity band in fibroblasts. To interrogate the Rho GTPase signaling flux, we build a genetic circuit consisting of an optogenetic actuator to control Rho activity, and a Rho activity biosensor. In spreading cells at steady state, optogenetic manipulation of Rho activity reveals that DLC1 controls the rate of Rho activation rather than duration, both at FAs and at the plasma membrane (PM). Local and reversible optogenetic control of contractility shows that DLC1 associates/dissociates with FAs during their reinforcement/relaxation. This might provide positive feedback that locally increases the rate of Rho activation at FAs that experience local tension to facilitate FA disassembly. Our results indicate that DLC1 operates both at the PM and at FAs to regulate global Rho activity levels at steady state, or to amplify local Rho activity at FAs experiencing a strong mechanical input, presumably to induce robust FA disassembly. This provides new insights in the complexity of spatio-temporal Rho GTPase signaling. Copy rights belong to original authors. Visit the link for more info Podcast created by Paper Player, LLC

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2023.04.05.535690v1?rss=1 Authors: Kirchenwitz, M., Halfen, J., von Peinen, K., Prettin, S., Kollasser, J., Brakebusch, C. H., Rottner, K., Steffen, A., Stradal, T. Abstract: Salmonella enterica serovar Typhimurium manipulates cellular Rho GTPases for host cell invasion by effector protein translocation via the Type III Secretion System (T3SS). The two Guanine nucleotide exchange (GEF) mimicking factors SopE and -E2 and the inositol phosphate phosphatase (PiPase) SopB activate the Rho GTPases Rac1, Cdc42 and RhoA, thereby mediating bacterial invasion. S. Typhimurium lacking these three effector proteins are largely invasion-defective. Type III secretion is crucial for both early and later phases of the intracellular life of S. Typhimurium. Here we investigated whether and how the small GTPase RhoB, known to localize on endomembrane vesicles and at the invasion site of S. Typhimurium, contributes to bacterial invasion and to subsequent steps relevant for S. Typhimurium lifestyle. We show that RhoB is significantly upregulated within hours of Salmonella infection. This effect depends on the presence of the bacterial effector SopB, but does not require its phosphatase activity. Our data reveal that SopB and RhoB bind to each other, and that RhoB localizes on early phagosomes of intracellular S. Typhimurium. Whereas both SopB and RhoB promote intracellular survival of Salmonella, RhoB is specifically required for Salmonella-induced upregulation of autophagy. Finally, in the absence of RhoB, vacuolar escape and cytosolic hyper-replication of S. Typhimurium is diminished. Our findings thus uncover a role for RhoB in Salmonella-induced autophagy, which supports intracellular survival of the bacterium and is promoted through a positive feedback loop by the Salmonella effector SopB. Copy rights belong to original authors. Visit the link for more info Podcast created by Paper Player, LLC

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2022.10.17.512253v1?rss=1 Authors: Mahlandt, E. K., Palacios Martinez, S., Arts, J. J. G., Tol, S., van Buul, J. D., Goedhart, J. Abstract: The inner layer of blood vessels consists of endothelial cells, which form the physical barrier between blood and tissue. This vascular barrier is tightly regulated to allow the passage of essential molecules like oxygen, carbon-dioxide, water, ions, and nutrients. The vascular endothelial barrier is defined by cell-cell contacts through adherens and tight junctions. To further investigate the signaling in the endothelium that regulates vascular barrier strength, we focused on Rho GTPases, regulators of the actin cytoskeleton and known to control junction integrity. Rho GTPase signaling is confined in space and time. To manipulate the signaling in a temporal and spatial manner we applied optogenetics. Guanine exchange factor (GEF) domains from ITSN1, TIAM1 and p63RhoGEF, activating Cdc42, Rac and Rho respectively, were integrated into the optogenetic recruitment tool iLID. This tool allows for activation at the subcellular level in a reversible and non-invasive manner and thereby to recruit a GEF to local areas at the plasma membrane, enabling the local activation of specific Rho GTPases. The membrane tag of iLID was optimized and a HaloTag was applied to gain more flexibility for multiplex imaging. The resulting Opto-RhoGEFs were tested in an endothelial cell monolayer and demonstrated precise temporal control of vascular barrier strength by a cell-cell overlap-dependent, VE-cadherin-independent, mechanism. Furthermore, Opto-RhoGEFs enabled precise optogenetic control in endothelial cells over morphological features such as cell-size, -roundness, local extension, and cell contraction. In conclusion, we have optimized and applied the optogenetic iLID GEF recruitment tool i.e. Opto-RhoGEFs, to study the role of Rho GTPases in the vascular barrier of the endothelium and found that membrane protrusions at the junction region can rapidly increase barrier integrity independent of VE-cadherin. Copy rights belong to original authors. Visit the link for more info Podcast created by Paper Player, LLC

Restoration of the Saints (9) (Audio) David Eells - 9/11/22 The Divine Image Destroyed May 4, 2021 - By Christine Beadsworth  Below is a portion of this blog page by Christine Beadsworth: The Holy Spirit has been speaking much to me about the forceful drive being perpetuated worldwide to introduce a purported medicine via needle into every human on the planet. I have received a number of dreams bringing revelation and understanding and I feel led to share these things in a series of posts. He started by showing me the whole scenario from God's point of view as He watches His beautiful creation being sabotaged. The visual picture which the Holy Spirit used was a wonderfully crafted palace, surrounded by a high wall. This amazing edifice was guarded day and night from intruders and only the King and those carefully vetted attendants serving him were allowed access. Then one day, a very destructive device was smuggled into the palace by a trusted page boy and the king was unaware that right within the Throne-room, the enemy had positioned one of his henchmen. We can see the same scenario in the positioning of Haman right next to king Xerxes. Oblivious to the danger, king Xerxes handed Haman his signet ring and gave him permission to send out a death decree (Esther 3:8). The human cell nucleus is that walled palace. The human cell is created with a nucleus wall to protect the scroll of DNA within its nucleus. Just like a wall is built around a palace to protect the ruler of a nation, these very important unique documents (our books or DNA scrolls) are guarded from marauders. Nothing is allowed to breach that wall and only trusted messengers are permitted to enter or leave that hallowed place. From within the nucleus, orders are issued by the DNA and carried by the messenger RNA through the nucleus wall to the golgi apparatus, which is a factory making proteins to build and strengthen the body. No other substance can pass through that wall except human mRNA. It is considered trustworthy to carry and deliver the messages issued.  I once heard a plant virologist describe mRNA like a shopping trolley. You can load whatever you want in it and it will transport it to the intended destination. Now this works well when the human body is not interfered with. We are fearfully and wonderfully made. MRNA faithfully carries the instructions issued by the unique DNA dwelling in the nucleus citadel. Psa 139:14-16  I will praise You; for I am fearfully and wonderfully made; Your works are marvellous and my soul knows it very well. 15  My bones were not hidden from You when I was made in secret and skillfully formed in the lowest parts of the earth. Psa 16 Your eyes saw my unformed substance, and in Your book (scroll) all the days [of my life] were written (engraved) before ever they took shape, when as yet there was none of them. Now, did you know that every single strand of human DNA is marked with the name of God. “How?” you ask… Because there are sulphide bonds holding together the two strands of DNA and they occur after the 10th pair of nucleotides, again after the 5th pair of nucleotides, then 6 pairs later and then again 5 pairs later. The 10th letter of the Hebrew alphabet is yod. The 5th letter is hey. The 6th letter is vav. Yod, hey, vav, hey spells ‘Yahweh'. I will say it again – God's name is on every human chromosome. You are made in His image and sealed with the Name of your Creator. Every cell in your body is hallmarked! And it is this very Divine hallmark that the enemy desires to obliterate. By injecting loaded messenger RNA into cells, one can sneak fragments of foreign enemy DNA through the carefully guarded nuclear cell walls and unload their contents into the scroll of DNA that God wrote for you. That's like pasting some pages of a counterfeit author's book into the middle of the instruction blueprint God wrote. What is actually happening is that the name of God written into the sulphide bond arrangement in the DNA scroll is being erased. The beautiful scroll engraved by God to create a man in His image is being sabotaged and from that moment of splicing onward, instead of instructions being released from the palace for life and health and security of the body, the inserted DNA loads up the mRNA trolley with instructions for the protein factory in the cell outside the palace to start manufacturing enemy spike proteins. The protein factory just does what it is told. Whatever orders arrive from headquarters are immediately carried out. To put it simply, once the potion is injected into the body, every single citadel guarding scrolls signed with the Name of the Creator within you is highjacked and forced to issue instructions to manufacture weapons of death – which will not only kill you at some point, but also the people around you. It is a cleverly engineered coup on a worldwide scale to throw off the rulership of God over His creation. So who is behind this dastardly plot? What does Psalm 2 say? Psa 2:2-3  The kings of the earth set themselves, and the rulers take counsel together, against the LORD, and against his anointed, saying, 3  Let us break their bands asunder, and cast away their cords from us.  The word for ‘band' means ‘bond, halter, restraint'. Is it not the carefully placed sulphide bonds that depict Yahweh's Name which are disrupted and broken asunder by the vaccine contents? The word ‘cord' in Hebrew also means ‘entwined, rope, thick boughs'. Is the DNA not entwined like a rope? Perhaps you can now see the plan of the kings of the earth; the elite, who are banding together and arraying themselves against the Lord. The plan is to highjack His creation, destroy His image, remove His signature and transform the human body into a genetically modified part human/part computer, which can be controlled externally. Sounds like science fiction, but tragically it is the truth. Satan hates all who are made in God's image; every earthen vessel that is intended to become a dwelling place of His Spirit, receiving daily communication from the Throne room of Heaven. For centuries he has been content to defile the bodies and souls of man by luring them into sin of every kind, but, always, his greatest ambition has been to sit in the place intended only for God in the human body. 2 Th 2:4 says that his intention is to ‘sit as God in the temple of God, setting himself forth as if he is God'.  Many are waiting for the third temple to be built so that the antichrist can go and sit in it, but THE BODY OF CHRIST IS THE 3RD TEMPLE; a temple not built with hands – and the diabolical deception currently underway is the first step in the rollout of the plan to install the abomination that causes desolation right within the holy place of the 3rd temple! In the holy place, the place where the unique engraved scroll of your Creator is stored in a walled citadel, the enemy has already lifted up his battle cry.  How apt is the cry of the psalmist in Psa 74:3-11  Direct Your feet [quickly] to the perpetual ruins and desolations; the foe has devastated and desecrated everything in the sanctuary. 4  In the midst of Your Holy Place Your enemies have roared [with their battle cry]; they set up their own [idol] emblems for signs [of victory]. 5  They seemed like men who lifted up axes upon a thicket of trees to make themselves a record. 6  And then all the carved wood of the Holy Place they broke down with hatchets and hammers.  7  They have set Your sanctuary on fire; they have profaned the dwelling place of Your Name by casting it to the ground. …10  O God, how long is the adversary to scoff and reproach? Is the enemy to blaspheme and revile Your name forever? 11  Why do You hold back Your hand, even Your right hand? Draw it out of Your bosom and consume them [make an end of them]!  ‘They have profaned the dwelling place of Your Name' — there is no better description of what is happening worldwide in the human body than these words. And in every nation,  believers are being coerced into taking the supposed health inducing jab. Is this not causing the ‘great falling away' described in 2 Th 2:3? The totality of the scheme is to leave the 3rd temple desolate, destroyed, overtaken and subjugated to an ancient enemy who always coveted God's throne and the worship of God's Bride. Apostasia, the Greek for ‘falling away', means ‘defection from truth' and comes from Strongs G647, which means ‘to separate, the writing of divorcement'. How significant that a fragment of DNA writing is what separates or breaks asunder the record of God's Name in the human DNA! Now obviously the smuggling in of the death warrant to the guarded throne room involves deception and subterfuge. No-one would allow such an intrusion if it was advertised by its true content. The coup is enacted under the guise of delivery of a basket of wishes for health and happiness. (Pity, there is a poisoned apple in the fruit bowl!) Revelation chapter 18 speaks of the judgement that is incurred by those who carry out this deception. As you read the selected verse, remember that the Greek word translated ‘sorceries' is pharmakeia, the word from which we get ‘pharmacy, dispenser of medicine', Rev 18:23b  …For your merchants were the great ones of the earth; for by your sorceries all nations were deceived.  So it is pharmaceutical deceptions which are employed to pull the wool over the eyes of all the nations. 2 Th 2:11 says a ‘strong delusion' is released so that people believe a lie. The Greek for ‘delusion' comes from a root word meaning ‘deceiver or imposter'. So the recurring theme is deception which brings destruction. No surprise that pharmakeia also means ‘witchcraft', and comes from a root word meaning ‘drug, poisoner'. Poison is intended to induce the death of the victim.  The Word tells us that the life is in the blood. It is surely no coincidence that the foreign spike proteins – which the human body is induced to produce after it is highjacked, trigger blood clots; which in turn cause fatal heart attacks and strokes. It is satan who seeks to steal, kill and destroy. Jesus came that you may have life and have it abundantly. Satan desires to remove God's image and replace it with his image (Rev 14:9). He plans to remove God's name and replace it with his name (Rev 14:11). Ask yourself who else could be behind the erasing of God's Name in your DNA scroll. Beloved, God says His people perish for lack of knowledge. Many pastors and shepherds worldwide are urging their congregations to receive the phamakeia they offer. May the sheep wake up and see the edge of the precipice before they plunge over it. I would urge you to guard and keep your temple holy unto the Lord. Do not allow it to be breached. Cherish His Name encoded into the scroll of life within you and take refuge under the shadow of His wings.  Do not run to Egypt or Babylon for help. Do all you can to boost your divinely built immune system… and do not allow yourself to be deceived. Be a lover of Truth. Seek truth. Go after it. 2 Th 2 is clear that it is those who do not love truth who will fall prey to the strong delusion wrapping its fingers around the globe. Do not succumb to those who call evil good and scheme to overthrow and render your holy place desolate. You will pay for it with your life. Rather entrust yourself to Him Who is the Truth, the Life and the way. Choose life that you and your children may live! 2Co 6:16-18  And what agreement does a temple of God have with idols? For you are the temple of the living God, as God has said, “I will dwell in them and walk among them; and I will be their God, and they shall be My people.” 17  Therefore come out from among them and be separated, says the Lord, and DO NOT TOUCH THE UNCLEAN THING. And I will receive you 18  and I will be a Father to you, and you shall be My sons and daughters, says the Lord Almighty.  Is This Why They Want to Change our DNA? Transcript of Rumble Video: Uncensored Sam - 9/9/21   God's true name is recorded within our DNA. So, just let me go ahead and explain. Our chains of DNA contain four nucleotide-based pairs called Adenine, Guanine, Thiamine, and Cytosine. And then there's a unit within the DNA strand called a sulfuric bridge. This sulfuric bridge holds our entire DNA strand together. It only appears after every ten nucleic acids have been laid down, and then after every five nucleic acids, then after every six, and then again, after every five nucleic acids.  So, we get this repeating numerical value of 10, 5, 6, 5 within our DNA strand. Well, it turns out the Biblical name for God, YHWH, contains the same numerical value as our DNA strand. Yod He Vav He equals ten, five, six, five. What's even more interesting is that if you take the Hebrew characters of the  name of YHWH and place it on its side, you get the image of a human being. Part three of this video series suggests that DNA is a language. Many people were wondering what “god" made the language of DNA.  Researchers at Harvard University announced today that they have found what appears to be a message from God written inside the human genome. In a little-explored section of non-coding DNA, a team of top geneticists discovered a 22-word snippet of ancient Aramaic in which God confirms His existence and His role in creating life on Earth. “We knew the patterns weren't naturally occurring,” explains Charles Watson, the lead scientist on the project, “but we couldn't come up with any convincing explanation for them.  On a whim, we started cross-referencing the patterns with language databases,” he explains, “and we were shocked to find that the patterns corresponded to ancient Aramaic.”  Stunned by its discovery, the team contacted language experts familiar with Aramaic – the language Jesus Christ spoke in daily life. Entirely decoded, the message reads: "Hello my children, This is Yahweh, the One True Lord. You have found creation's secret. Now share it peacefully with the world." Go tell the world Who is the true God and Creator of all! DNA: Good and Evil Seed Kenneth (David's notes in red) Here below is an amazing testimony of a 16-year-old young lady from South Africa. It is an astonishing confirmation of the battle of good and evil and how it lives within us from birth. It is again a supernatural confirmation of the truths found in the Bible from Genesis through Revelation. It is a confirmation of the Daniel 2:43 problem where "they" mingle themselves with the seed of man. GLORY TO JESUS. I pray this both edifies and blesses you with the reality of how our science, indeed God's science, is found in our Holy Bible. DNA Structure ~ A Word from I AM to His True Set-Apart Bride in these End Times - July 9, 2013 at 6:23am ... given through His beloved daughter, Sarah (16 years old) on 5th July 2013 ... Please NOTE that this Word from the mouth of Yahushua haMashiach is not specific to South Africa only, but is for His Set-apart Bride collectively. Yeshua stood next to me dressed in white light, He stretched out His pierced hand and said, "Come with Me, My Sarah Princess, My Deborah warrior"... It was as if we were eagles, flying over South Africa. He showed me different things in the country. I saw fires in many places. Yeshua smiled and said, "It's My fire; My Spirit is fanning the flame in the hearts of those who choose to walk the road less traveled, in those who choose to let go of the passions of the flesh, I am busy in this country you call home, My love. There are many things to come as you know". I see a big statue of Nelson Mandela with a rainbow around it. Yeshua looked at it and turned His face... "Sarah, look at the way people make an idol out of man - REPENT THEREOF". Then He said, "The nation's pride will come to a fall". I saw the statue slowly diminishing on the inside until there was nothing left on the inside of the statue, but it was still standing. I saw people crying, lots of people stood in groups and cried. "Tell those who so humbly and faithfully stand in the gap for this nation, pray and plead for this nation that they will soon start to see the fruit of their supplication". While He said that, I saw many people praying and repenting for the things in this country. (This should be done in whatever nation you are. The fruit of that worldly nation will be the elect of God.) "My love, remember I said in My Book, I put before you light and darkness, blessing and curse, and you have the choice; which one do you choose? You are between two Kingdoms, My love. At the same time I am busy, the wicked one is also busy... I AM shining My glorious light through the words of My prophets and messengers and those who give ear will receive blessing, the blessing of knowing the truth and having it make you free. I want to speak to you about what I am busy with so that My people can catch up with My heartbeat, so that they can dance in the rhythm of My ways, My truths, My desires... "I am busy gathering My bride into the ark where she will be safe. It is only those who have cleansed their DNA, those who have pure blood that can enter. Sarah, tell My people to give attention to what I will tell you now; it is very important, it is a matter of purity and harlotry, salvation and deception, luke-warmness and fire... Give ear!" The next moment, it was as if I was inside of a body and I see conception taking place, the sperm cell and the egg cell colliding, merging and 'becoming' a baby. I see the seed in very much detail; there were these little things like white vessels that looked like trees inside the seed (family trees, our genetic inheritance from our parents in the world).  At the same time, while I was looking at that, I also saw that the seed is like a book and there are many, many words inside that very tiny seed. (Words pass along to our physical seed, cursing or blessing, by our nature). (The seed of the spiritual man is like a book also, the Bible. Our meditation on the Word empowers and releases the spiritual man to control the physical man and the demons.) (Kenneth's comment: The Psalmist wrote: Thine eyes did see my substance, yet being unperfect (unformed); and in thy book all my members were written, which in continuance were fashioned, when as yet there was none of them. (Psalm 139:16) Yeshua started to speak to me about the DNA once again... "My love, do you see? Look at it; it's beautiful... out of these little tiny seeds a human grows, inside of these little seeds I put everything for a baby to grow; it is perfect (it was very, very special to see Yeshua so excited about how perfect and amazing and AWESOME He made the human body, soul and spirit). Look inside the seed, do you see the written things? It is a code, it's a DNA code; it's far above what any human can understand.  That code, My love, determines who that baby will be, that code will form its character, personality, likes and dislikes. It forms the body and it forms the soul, but not the spirit. The spirit is born of Spirit, My Spirit. In your spirit you inherit My DNA when you are saved, but this DNA, this seed and inheritance, is not saved yet. Sarah, inside of that seed is blessing and curse, sin and righteousness as an inheritance from your fathers. (For this reason mad scientists want to recreate the DNA after their own liking by perverting the food, water, and air and by va/cci/nes. This way they can create the body of the beast.) "This DNA is very, very important. Inside of the DNA code, there are supposed to be key essentials so that your body and soul can function the way I purposed them to. These seeds in the DNA are love proteins, and when you are born, the seed will sprout if it is exposed to the right elements. The mother and father are to nurture, comfort, affirm, accept and love this gift from Me and the result will be gender identity and spiritual identity, a healthy and whole soul and body.  But now the babies are being exposed to trauma - even at birth - with the way this precious child comes into this world. The children are vulnerable to whatever the parents expose them to, even in the womb, and it has a great effect on them. (Speak blessing over the unborn.) It is the parents' work to build the child's identity through teaching them to find their identity in Me, but they are exposed to the trauma of rejection (opening them up to demonic bondage)... "This DNA speaks all the time. It speaks of every word written inside of it. You can hear it and I can hear it too; you hear it in your mind every day. Sarah, your inherited DNA affects your soul and body in a great, great way. Let Me explain to you how it affects you in a physical and mental way. Your soul area is your mind, will, emotions, desires.  The seed in your DNA grows like trees throughout your soul. For example, if you inherited a seed of rejection, that seed will carry the hurts and pain of the generations before you. (We have seen this to be true. Many don't know why they are the way they are, always lusting for acceptance and admiration from others. Rejecting any correction or person that knows about them.) That seed is in you and when you experience any kind of trauma, trauma at birth, as a child, or any kind of trauma, the seed sprouts and a stronghold forms inside your soul. (Any perceived threat to the ego puts them in survival mode.)  A stronghold forms to suppress the pain. The stronghold is always rooted in pride and fear keeps it in place. These trees are rebellion against Me. This tree bears ungodly fruit in your soul like perfectionism, judgment, fear, pride ... (Rejection is bearing much fruit in these days to separate the tares from the wheat but repentance is possible through faith in Jesus' sacrifice. These two seeds are manifested through one's actions and words. {Mat.13:37} And he answered and said, He that soweth the good seed is the Son of man; {38} and the field is the world; and the good seed, these are the sons of the kingdom; and the tares are the sons of the evil [one]; {39} and the enemy that sowed them is the devil: and the harvest is the end of the world; and the reapers are angels. {40} As therefore the tares are gathered up and burned with fire; so shall it be in the end of the world. {41} The Son of man shall send forth his angels, and they shall gather out of his kingdom all things that cause stumbling, and them that do iniquity, {42} and shall cast them into the furnace of fire: there shall be the weeping and the gnashing of teeth. {43} Then shall the righteous shine forth as the sun in the kingdom of their Father. He that hath ears, let him hear. "Because I love you so dearly and because you struggled so much as a little girl to understand why 'this Father of love' would create imperfect, disabled little babies, I will show you why this happens. "This seed of rejection, for example, can affect your body in many ways; sometimes the sickness manifests in a later stage because of trauma and other times because of open doors in the mother and father. Because of generational curses, spirits latch onto the DNA and the baby can be born with a defect, a disease or disabled. It is part of the curse that came upon man at the fall. (However, a believing parent sanctifies the child through faith, as Paul said in 1 Corinthians 7, and can deliver the child from the curse of defect and disease.)  Sarah, there are many things I want to teach you about the DNA. My people have a lack of knowledge! I also want to teach you about the DNA structure: It is an order. There are two orders: the one comes out of hell and the other out of Who I AM! Out of hell comes The Order of Baal and man plays the harlot with it, and I will call My order The Order of I AM! This is the structure in your DNA. "Inside The Order of Baal is the structure, the nature and the seed of lucifer. Curse is a result of it, most sicknesses are a result of it, spiritual death is a result of it, poverty is a result of it and destruction is a result of it. I have spoken to you a lot about the pure bride and about what it means to be the pure bride. The pure bride has My DNA, she has My structure, My order, My nature and My seed in her. Blessing is a result of it, life is a result of it, prosperity is a result of it, holiness and righteousness are a result of it. "My bride has I AM flowing through her veins, while the harlot bride, who has not cleansed her DNA, has hell operating through her … a mixed seed. My people are playing harlot because they have the order and the structure of Baal in them -that is why they are double-minded, that is why they are still sick. Even though by My stripes you were healed, they have remained still sinful, unholy and unrighteous, but they call themselves Christians. I tell you the word Christian is (misused and is) reserved for those who are lukewarm.  You are either My bride, holy, pure and righteous, building your spirit house upon the Rock of Ages, living in My Kingdom, or you are a 'Christian,' lukewarm, so-called saved but deceived, so-called good but led astray without even knowing it. This 'Christian' is part of the harlot; she is in fact worse than the sinner because she is the same as the sinner in heart, but she thinks she is good. "My people, if you want to go into the ark like Noah, you have to cleanse your DNA through applying My blood through repentance, through petitioning in My heavenly courtroom. Remember, your spirit can be holy and righteous and you can think you are on the right road because of it. But if you do not take the next step through cleansing your DNA and working on your soul dimension, you are divided within yourself - in your spirit, on fire, and in your soul, cold, and therefore you are mixed, mixed and lukewarm. My bride is not mixed, she is single-minded and in unity with Me, one in soul and spirit. (She is one with the Word in her heart and obedience to it.) "I want My people to break down the altar, the order and the structure of Baal in your blood (DNA) that your forefathers have cast up. Like Gideon had to break down the altar of Baal. ("Come out of her My people".) {Jdg.6:25} ... Tear down the altar of Baal that your father has, and cut down the wooden image that is beside it; {26} and build an altar, to the LORD your God on top of this rock laid in proper order [proper arrangement]. I was petitioning and repenting for my DNA and while I did that I saw a vision of inside of my body, I saw the many blood vessels, and Yeshua took me inside of my blood where He showed me how my DNA looks in the spirit. I saw the structure of the DNA and I saw two doors inside of my DNA (blood). There was a black door and a golden door. The golden door was closed and the black door was open. Out of the black door the DNA structure flowed; on the black door was written "Order of Baal/Curse" and on the golden door was written "I AM/Blessing" (which is filled with the FULLNESS of ABBA's character, attributes, nature, in the name of I AM is the fullness of who He is). There were things written on the black structure that flowed out of the black door. I recognized it as pride and rebellion against I AM (everything YHVH stands for), the nature of hasatan. So I started to pray for I AM's light to shine in my DNA and I just started to repent of generational sin as Ruach haKodesh revealed it to me. He went really deep and I carried on for hours. While I was busy repenting, I saw the structure going back into the black door, like it was being reversed. I literally saw Yeshua inside of my blood; He closed the black door and the door and the structure disappeared (shattered the order of Baal and curses). It is as if the black door was a gateway of hell. Yeshua said, "Each and every human has these two doors in their blood; hell operates through the black door through pride, rebellion and curses - it is his nature flowing out of it; while I AM and blessing flows through the way, the truth and the life. (Jesus comes through the spirit man and Satan and his minions through the inherited flesh and its lusts.)  My people, you are in a DNA war. Awaken and see that the battle you are in is a blood battle; the enemy is out to defile your blood. Break down The Order of Baal inside of you through repentance and petitioning. Your DNA is who you are; it is the core of you. I want who you are to be a vein, a vessel for I AM, for I AM to flow through you and be an altar of worship, singing holy (set-apart), holy (set-apart), holy (set-apart), holy (set-apart)!" While He said that, the golden door flung open and a white structure flowed out, with golden and silver writings on it. I AM's character and nature was inside of it - humility, holiness(set-apartness), love, gentleness, etc. "Sarah, My love, this is the message for this hour. Teach My people how to sanctify their DNA because this is what My bride looks like". I see what flows out of that door is His seed [love and righteousness].  If it is the black door, it is hasatan's seed [pride and rebellion]. Father's seed births purity and holiness (set-apartness) and results in a set-apart virgin bride, whereas satan's seed births pride, harlotry, idolatry - it is mixed seed - and this makes you a harlot bride that can give birth to Nephilim babies. (Meaning dark spiritual giants, a mixture of flesh and demons; people who are big in the world because they are of the world, and they are small before God, like famous false Christians.) Yeshua showed me that the written things on our DNA become what our mind meditates upon. (We can meditate on flesh or spirit and bear the fruit thereof. {Isa.26:3} Thou wilt keep [him] in perfect peace, [whose] mind [is] stayed [on thee]; because he trusteth in thee. Rom 12:2  And be not fashioned according to this world: but be ye transformed by the renewing of your mind, that ye may prove what is the good and acceptable and perfect will of God.)  Your mind literally records whatever is inside the DNA. For example, when there is generational racism seed in your DNA, you have a judgmental, prideful, racist stronghold in your mind (of course you don't acknowledge it as that). Because you inherit the seed from your fathers, it hides in you and when trauma occurs it explodes like a bomb in your soul. It is the same with homosexuality; if there is a seed of rejection, to which a spirit latches, that attacks and breaks down your identity physically and because there is no love inside of that seed, it can very easily manifest in one becoming homosexual. The homosexual spirit is the stronghold that the structure of your soul puts up to defend itself, but it comes out of a seed of rejection, out of the pits of hell. (Many other spirits use the spirit of rejection as a doorkeeper to let them in: rebellion, anger, frustration, domineering, Jezebel, witchcraft, faction, drunkenness, drug addiction, self-seeking, etc.) We are in a spiritual battle that manifests in the natural and the war is already won, if we would just start to apply the Blood of the Lamb, we would start to taste victory and freedom. Our weapon is the BLOOD OF THE LAMB (which carries Yeshua's DNA) because it is the fullness of I AM's LOVE, poured out for our salvation, redemption, deliverance, healing, freedom... REPENT, so that you can be called righteous like Noah was righteous in all his generations! See also Deliverance from Rejection and Its Fear UBM site link: dna Restoration of the Body Tommy Hicks - 7/25/61 (David's notes in red) The following is an excerpt of a stunning vision given to American preacher Tommy Hicks, who was a major figure in the powerful 1954 Argentina Revival. VISION OF THE BODY OF CHRIST AND THE END-TIME MINISTRIES “…In the book of Acts we read, "In the last days", God said, "I will pour out my Spirit upon all flesh". I wonder if we realized what he meant when God said, "I will pour out my Spirit upon all flesh". I do not think I fully realized nor could I understand the fullness of it, and then I read from the book of Joel: "Be glad then, ye children of Zion, and rejoice in the Lord your God: for he hath given you the former rain moderately, and he will cause to come down for you the rain, the former rain, and the latter rain -" (Joel 2:23). It is not only going to be the rain, the former rain and the latter rain, but he is going to give to his people in these last days a double portion of the power of God! As the vision appeared to me after I was asleep, I suddenly found myself in a great high distance. Where I was, I do not know. But I was looking down upon the earth. Suddenly the whole earth came into my view. Every nation, every kindred, every tongue came before my sight from the east and the west, the north and the south. I recognized every country and many cities that I had been in, and I was almost in fear and trembling as I beheld the great sight before me: and at that moment when the world came into view, it began to lightning and thunder. As the lightning flashed over the face of the earth, my eyes went downward and I was facing the north. Suddenly I beheld what looked like a great giant, and as I stared and looked at it, I was almost bewildered by the sight. It was so gigantic and so great. His feet seemed to reach to the North Pole and his head to the south. Its arms were stretched from sea to sea. I could not even begin to understand whether this was a mountain or a giant, but as I watched, I suddenly beheld a great giant. I could see his head was struggling for life. He wanted to live, but his body was covered with debris from head to foot, and at times this great giant would move his body and act as though it would even rise up at times. And when it did, thousands of little creatures seemed to run away. Hideous creatures would run away from this giant, and when he would become calm, they would come back. (These are the demons that are keeping God's people in bondage.) All of a sudden this great giant lifted his hand towards heaven, and then it lifted its other hand, and when it did these creatures by the thousands seemed to flee away from this giant and go into the darkness of the night. Slowly this great giant began to rise and as he did, his head and hands went into the clouds. As he rose to his feet he seemed to have cleansed himself from the debris and filth that was upon him, and he began to raise his hands into the heavens as though praising the Lord, and as he raised his hands, they went even unto the clouds. (Representing the great revival through Jesus in the Man-child reformers.) Suddenly, every cloud became silver, the most beautiful silver I have ever known. As I watched this phenomenon it was so great I could not even begin to understand what it all meant. I was so stirred as I watched it, and I cried unto the Lord and I said, "Oh Lord, what is the meaning of this". and I felt as if I was actually in the Spirit and I could feel the presence of the Lord even as I was asleep. And from those clouds suddenly there came great drops of liquid light raining down upon this mighty giant, and slowly, slowly, this giant began to melt, began to sink itself in the very earth itself, and as he melted, his whole form seemed to have melted upon the face of the earth, and this great rain began to come down. Liquid drops of light began to flood the very earth itself and as I watched this giant that seemed to melt, suddenly it became millions of people over the face of the earth. As I beheld the sight before me, people stood up all over the world! They were lifting their hands and they were praising the Lord. At that very moment there came a great thunder that seemed to roar from the heavens. I turned my eyes toward the heavens and suddenly I saw a figure in white, in glistening white - the most glorious thing that I have ever seen in my entire life. I did not see the face, but somehow I knew it was the Lord Jesus Christ, and he stretched forth his hand, and as he did, he would stretch it forth to one, and to another, and to another. And as he stretched forth his hand upon the nations and the people of the world - men and women - as he pointed toward them, this liquid light seemed to flow from his hands into them, and a mighty anointing of God came upon them, and those people began to go forth in the name of the Lord. I do not know how long I watched it. It seemed it went into days and weeks and months. And I beheld this Christ as he continued to stretch forth his hand; but there was a tragedy. There were many people as he stretched forth his hand that refused the anointing of God and the call of God. I saw men and women that I knew. People that I felt would certainly receive the call of God. But as he stretched forth his hand toward this one and toward that one, they simply bowed their head and began to back away. And each of those that seemed to bow down and back away, seemed to go into darkness. Blackness seemed to swallow them everywhere. (Their sin was more valuable to them than the anointing.) I was bewildered as I watched it, but these people that he had anointed, hundreds of thousands of people all over the world, in Africa, England, Russia, China, America, all over the world, the anointing of God was upon these people as they went forward in the name of the Lord. I saw these men and women as they went forth. They were ditch diggers, they were washer women, they were rich men, and they were poor men. I saw people who were bound with paralysis and sickness and blindness and deafness. As the Lord stretched forth to give them this anointing, they became well, they became healed, and they went forth! And this is the miracle of it - this is the glorious miracle of it - those people would stretch forth their hands exactly as the Lord did, and it seemed as if there was this same liquid fire in their hands. As they stretched forth their hands they said, "According to my word, be thou made whole". As these people continued in this mighty end-time ministry, I did not fully realize what it was, and I looked to the Lord and said, "What is the meaning of this?" And he said, "This is that which I will do in the last days. I will restore all that the cankerworm, the palmerworm, the caterpillar - I will restore all that they have destroyed. This, my people, in the end times will go forth. As a mighty army shall they sweep over the face of the earth”. (Representing the great restoration.) As I was at this great height, I could behold the whole world. I watched these people as they were going to and fro over the face of the earth. Suddenly there was a man in Africa and in a moment he was transported by the Spirit of God, and perhaps he was in Russia, or China or America or some other place, and vice versa. All over the world these people went, and they came through fire, and through pestilence, and through famine. Neither fire nor persecution, nothing seemed to stop them. Angry mobs came to them with swords and with guns. And like Jesus, they passed through the multitudes and they could not find them, but they went forth in the name of the Lord, and everywhere they stretched forth their hands, the sick were healed, the blind eyes were opened. There was not a long prayer, and after I had reviewed the vision many times in my mind, and I thought about it many times, I realized that I never saw a church, and I never saw or heard a denomination, but these people were going in the name of the Lord of Hosts. Hallelujah! As they marched forth in everything they did as the ministry of Christ in the end times, these people were ministering to the multitudes over the face of the earth. Tens of thousands, even millions seemed to come to the Lord Jesus Christ as these people stood forth and gave the message of the kingdom, of the coming kingdom, in this last hour. It was so glorious, but it seems as though there were those that rebelled, and they would become angry and they tried to attack those workers that were giving the message. (As it was with the Pharisees and Sadducees.) God is going to give the world a demonstration in this last hour as the world has never known. These men and women are of all walks of life, degrees will mean nothing. I saw these workers as they were going over the face of the earth. When one would stumble and fall, another would come and pick him up. There were no "big I's" and "little you's", but every mountain was brought low and every valley was exalted, and they seemed to have one thing in common - there was a divine love, a divine love that seemed to flow forth from these people as they worked together, and as they lived together. It was the most glorious sight that I have ever known. Jesus Christ was the theme of their life. They continued and it seemed the days went by as I stood and beheld this sight. I could only cry, and sometimes I laughed. It was so wonderful as these people went throughout the face of the whole earth, bringing forth in this last end time. As I watched from the very heaven itself, there were times when great deluges of this liquid light seemed to fall upon great congregations, and that congregation would lift up their hands and seemingly praise God for hours and even days as the Spirit of God came upon them. God said, "I will pour my Spirit upon all flesh", and that is exactly this thing. And to every man and every woman that received this power, and the anointing of God, the miracles of God, there was no ending to it…" UBM Angels Give Some Details of the Coming Great Revival Excerpt from Ministering Angels Through UBM #3 David asked, “What can you tell us about the supernatural methods to get the gospel out and heal, deliver and save?” The angel Baruch said, "The methods of man will not be employed to reach the peoples of the earth. (In the days that are coming.) The methods are all of the spirit realm and will be administered by the angels who are going to distribute the gifts to the saints. (Meaning, the sanctified ones) These include gifts of translation from place to place, language, healing, laying on of hands to impart to others their needs and what they're lacking and to replicate the gifts by passing them on to others whom the angels will point out. Supernatural downloads of wisdom and knowledge will be received for specific situations.”  David asked, “What about deliverances?” Baruch said, “The enemy will be on the run during this time. Deliverances will be instantaneous with no resistance on their part. (Many deliverances are this way now if there is cooperation on the part of the victim in repentance and faith.) They will no longer be able to hide in flesh because of the light of revival and the fire of truth that will sweep the elect of the globe.” (Eve said, “Meaning: Demons will be fleeing left and right out of people as the true fire of the Holy Spirit fills each one and searches the depths of each individual. There won't be any place left for them to hide in the elect of God.") David asked, “Will there be mass healings and deliverances? Baruch said, “ Yes. These will all take place on the largest scale in human history.” (Eve said, "Meaning: Masses of people will supernaturally be delivered and healed all at once for times sake because the need is so great and the darkness so pervasive.") Then the angel Shemuel said, "A great move of God is coming upon the younger generations. (i.e. The millennials - Generation Z) There is a mildew and rottenness that has been cast upon this crop by satan; a perpetual darkness, so that the Son has not shown upon them. They languish and rot in the field and their fruit is poisoned. But the Son is coming to shine down upon them and he will burn up all the disease and mildew of their lives and the heat of His fire will burn through them and cleanse and restore them to the purpose they were created for. There has never been a revival like this that will sweep the whole earth. This will be entirely an act of God; supernatural and undeniable. He will not forsake the youth of this final generation.'  Our Promised DNA Restoration Excerpt from Ministering Angels Through UBM #3 David asked, “What does the coming restoration involve personally? 30 years younger?” The angel Jeruel answered, “Restoration on a DNA cellular level. Faith and power will be imputed to receive healing and restoration. It will be complete restoration not partial. The first fruits are first. They will be the trail blazers. There will also be a mighty supernatural outpouring of spiritual restoration of closeness to God through His Holy Spirit.” Then David asked, “When will the Lord restore our teeth?” The angel Baruch said, “When the DNA restoration takes place, teeth will be restored as well.” Our Corporate Birthday Party Isaac Payne - 8/19/22 (David's notes in red) In this dream I was hosting a birthday party at my doublewide in Florida. (I think Florida is symbolic due to it being called the sunshine state. Also, I think the doublewide represents John 1:16 For of his fullness we all received, and grace for grace. Double grace hence doublewide.) The walls of the doublewide were sky blue and pretty much everything in the doublewide was blue. (Blue represents heavenly.)  As the birthday party began, we welcomed many people inside. What was odd was that it wasn't anyone's birthday in particular, but actually seemed like a shared birthday party. (The corporate birthing of Jesus in the Man-child reformers and then the choosing of the Bride.) I looked around and saw Missy Pollock and her husband Merle at the birthday party sitting at the dining room table. They looked very happy and I remember thinking Merle is a really fit guy and in good shape and Missy too. (This is the timing of the DNA restoration.) I saw my Uncle Ronnie come to the party also. (Ronnie means ruler, advice, decision, bringer of victory.) (Representing the Father.) Also, David Eells (Representing the Man-child David's.) was there at the house for the birthday party as well. I continued to look around and also saw my cousins Josh and Jake. (Joshua means God is Deliverance and Jacob (Jacob represents Israel as a type of the Church.) (Ronnie, Josh, and Jake are my actual family members on my dads side in real life.)    I think this points to spiritual Israel since Jacob was renamed Israel. Romans 9:6 But it is not as though the word of God has come to nought, for they are not all Israel, that are of Israel. But also he took the birthright from Esau. I believe we have been given the birthright from Christ as we are the 42nd generation and have overcome the old man represented by Esau. 2 Co. 1:20 For however many so ever be the promises of God, in him is the yea: wherefore also through him is the Amen, unto the glory of God through us. Then I woke up. (Also, I believe this is confirmation for the DNA regeneration that we were told we would receive.) (There wasn't one feeble one among them when they went out into the wilderness. Moses' eye was not dimmed nor his natural force abated.) The Great Restoration David Eells  The Lord reveals that there will be a restoration of His people's lives in an outpouring of the Spirit after the Northern Army, as a type of the faction in the government and in the Church, is defeated at the spiritual Red Sea. This is primarily a restoration to the Bride, as a first-fruits, and later through the Bride to the Church. The Lord has showed us that this Northern Army attack symbolizes the ongoing D.S. attack on the U.S. using Pestilence, Sword, Famine, and Fire, which is listed in scriptures many times in Babylon's war against the apostate leadership, typed as Jerusalem, and God's apostate people...  The Northern Army of Assyria tried to conquer righteous Jerusalem, the Bride. They had already conquered the apostate northern tribes, which faction and apostates have done, because these conquered ones are not the Bride, who escaped. Here also is mentioned the restoration of the years this enemy had plundered from God's people.  Joel 2:20-27 but I will remove far off from you the northern army, and will drive it into a land barren and desolate, its forepart into the eastern sea, and its hinder part into the western sea; and its stench shall come up, and its ill savor shall come up, because it hath done great things.  21 Fear not, O land, be glad and rejoice; for Jehovah hath done great things. 22 Be not afraid, ye beasts of the field; for the pastures of the wilderness do spring, for the tree beareth its fruit, the fig-tree and the vine do yield their strength. 23 Be  glad then, ye children of Zion, and rejoice in Jehovah your God; for he giveth you the former rain in just measure, and he causeth to come down for you the rain, the former rain and the latter rain, in the first month.  24 And the floors shall be full of wheat, and the vats shall overflow with new wine and oil. 25 And I will restore to you the years that the locust hath eaten, the canker-worm, and the caterpillar, and the palmer-worm, my great army which I sent among you. 26 And ye shall eat in plenty and be satisfied, and shall praise the name of Jehovah your God, that hath dealt wondrously with you; and my people shall never be put to shame.  27 And ye shall know that I am in the midst of Israel, and that I am Jehovah your God, and there is none else; and my people shall never be put to shame. I believe this restoration incorporates the power of the Spirit, health, youth, prosperity, doctrine, fruit, gifts, authority, and a general deliverance from all of the curse that Jesus paid for.  Gal 3:13 Christ redeemed us from the curse of the law, having become a curse for us; for it is written, Cursed is every one that hangeth on a tree. This restoration will begin with the Bride who will spread this gospel to the true Church of the called out ones. Psa 103:1-5 A Psalm of David. Bless Jehovah, O my soul; And all that is within me, bless his holy name. 2 Bless Jehovah, O my soul, And forget not all his benefits: 3 Who forgiveth all thine iniquities; Who healeth all thy diseases; 4 Who redeemeth thy life from destruction; Who crowneth thee with lovingkindness and tender mercies; 5 Who satisfieth thy desire with good things, So that thy youth is renewed like the eagle.  (Eagles, like all other birds, molt. They molt four times before reaching adulthood.  Once they achieve their final "adult" plumage, it is likely that Bald Eagles molt their flight feathers just about every year. This represents new clothing which helps it overcome the gravity of the world.) Rom 13:14 But put ye on the Lord Jesus Christ, and make not provision for the flesh, to fulfil the lusts thereof. Job 33:25 His flesh shall be fresher than a child's; He returneth to the days of his youth. God's people coming out of Egypt at the time of the Red Sea received their youthful restoration. Psa 105:37 And he brought them forth with silver and gold; And there was not one feeble person among his tribes. Deu 34:7 And Moses was a hundred and twenty years old when he died: his eye was not dim, nor his natural force abated. Neh 9:21 Yea, forty years didst thou sustain them in the wilderness, and they lacked nothing; their clothes waxed not old, and their feet swelled not. 2Co 5:17 Wherefore if any man is in Christ, he is a new creature: the old things are passed away; behold, they are become new. Abiding in the mind of Christ, the Lions head, will bring about this new creation man. Psa 132:13-18 For Jehovah hath chosen Zion (the Bride); He hath desired it for his habitation. 14 This is my resting-place for ever: Here will I dwell; for I have desired it. 15 I will abundantly bless her provision: I will satisfy her poor with bread. 16 Her priests also will I clothe with salvation; And her saints shall shout aloud for joy. 17 There will I make the horn of David to bud: I have ordained a lamp for mine anointed. 18 His enemies will I clothe with shame; But upon himself shall his crown flourish. Psa 133:3 Like the dew of Hermon, That cometh down upon the mountains of Zion: For there Jehovah commanded the blessing, Even life for evermore. Act 3:19-21 Repent ye therefore, and turn again, that your sins may be blotted out, that so there may come seasons of refreshing from the presence of the Lord; 20 and that he may send the Christ who hath been appointed for you, even Jesus: 21 whom the heaven must receive until the times of restoration of all things, whereof God spake by the mouth of his holy prophets that have been from of old. Jesus will first come manifested in the Man-child reformation ministry to restore all that was stolen from God's people just as He did in His first body except on a much larger scale.  Hos 6:1-3 Come, and let us return unto Jehovah; for he hath torn, and he will heal us; he hath smitten, and he will bind us up. 2 After two days will he revive us: on the third day he will raise us up, and we shall live before him. 3 And let us know, let us follow on to know Jehovah: his going forth is sure as the morning; and he will come unto us as the rain, as the latter rain that watereth the earth. 1Pe 5:10 And the God of all grace, who called you unto his eternal glory in Christ, after that ye have suffered a little while, shall himself perfect, establish, strengthen you. Psa 71:20-21 Thou, who hast showed us many and sore troubles, Wilt quicken (meaning: Make alive) us again, And wilt bring us up again from the depths of the earth. 21 Increase thou my greatness, And turn again and comfort me. Jer 30:1-3 The word that came to Jeremiah from Jehovah, saying, 2 Thus speaketh Jehovah, the God of Israel, saying, Write thee all the words that I have spoken unto thee in a book. 3 For, lo, the days come, saith Jehovah, that I will turn again the captivity of my people Israel and Judah, saith Jehovah; and I will cause them to return to the land that I gave to their fathers, and they shall possess it. This speaks of the Church coming out of captivity to the beast kingdom to return to the Land of milk and honey; the land of our spiritual forefathers in the Gospels and Book of Acts. We are going to have our own Book of Acts but greater in scope and intensity. Isa 40:31 but they that wait for Jehovah shall renew their strength; they shall mount up with wings as eagles; they shall run, and not be weary; they shall walk, and not faint. What energy Father will put in His people to bring in the harvest. Jer 29:11-14 For I know the thoughts that I think toward you, saith Jehovah, thoughts of peace, and not of evil, to give you hope in your latter end. 12 And ye shall call upon me, and ye shall go and pray unto me, and I will hearken unto you. 13 And ye shall seek me, and find me, when ye shall search for me with all your heart. 14 And I will be found of you, saith Jehovah, and I will turn again your captivity, and I will gather you from all the nations, and from all the places whither I have driven you, saith Jehovah; and I will bring you again unto the place whence I caused you to be carried away captive. Back to the Bride we started from.  Joh 3:29 He that hath the bride is the bridegroom: but the friend of the bridegroom, that standeth and heareth him, rejoiceth greatly because of the bridegroom's voice: this my joy therefore is made full. Isa 60:10-22 And foreigners shall build up thy walls, and their kings shall minister unto thee: for in my wrath I smote thee, but in my favor have I had mercy on thee. 11 Thy gates also shall be open continually; they shall not be shut day nor night; that men may bring unto thee the wealth of the nations, and their kings led captive. 12 For that nation and kingdom that will not serve thee shall perish; yea, those nations shall be utterly wasted. 13 The glory of Lebanon shall come unto thee, the fir-tree, the pine, and the box-tree together, to beautify the place of my sanctuary; and I will make the place of my feet glorious.  14 And the sons of them that afflicted thee shall come bending unto thee; and all they that despised thee shall bow themselves down at the soles of thy feet; and they shall call thee The city of Jehovah, The Zion of the Holy One of Israel. 15 Whereas thou hast been forsaken and hated, so that no man passed through thee, I will make thee an eternal excellency, a joy of many generations. 16 Thou shalt also suck the milk of the nations, and shalt suck the breast of kings; and thou shalt know that I, Jehovah, am thy Saviour, and thy Redeemer, the Mighty One of Jacob.  17 For brass I will bring gold, and for iron I will bring silver, and for wood brass, and for stones iron. A great increase in value in life and posessions. I will also make thy officers peace, and thine exactors righteousness. 18 Violence shall no more be heard in thy land, desolation nor destruction within thy borders; but thou shalt call thy walls Salvation, and thy gates Praise. 19 The sun shall be no more thy light by day; neither for brightness shall the moon give light unto thee: but Jehovah will be unto thee an everlasting light, and thy God thy glory.  20 Thy sun shall no more go down, neither shall thy moon withdraw itself; for Jehovah will be thine everlasting light, and the days of thy mourning shall be ended. 21 Thy people also shall be all righteous; they shall inherit the land for ever, the branch of my planting, the work of my hands, that I may be glorified. 22 The little one shall become a thousand, and the small one a strong nation; I, Jehovah, will hasten it in its time. A great revival with multitudes coming into the Kingdom of God. Isa 61:1-11 The Spirit of the Lord Jehovah is upon me; because Jehovah hath anointed me to preach good tidings unto the meek; he hath sent me to bind up the broken-hearted, to proclaim liberty to the captives, and the opening of the prison to them that are bound; 2 to proclaim the year of Jehovah's favor, and the day of vengeance of our God; to comfort all that mourn; 3 to appoint unto them that mourn in Zion, to give unto them a garland for ashes, the oil of joy for mourning, the garment of praise for the spirit of heaviness; that they may be called trees of righteousness, the planting of Jehovah, that he may be glorified.  4 And they shall build the old wastes, they shall raise up the former desolations, and they shall repair the waste cities, the desolations of many generations. 5 And strangers shall stand and feed your flocks, and foreigners shall be your plowmen and your vine-dressers. 6 But ye shall be named the priests of Jehovah; men shall call you the ministers of our God: ye shall eat the wealth of the nations, and in their glory shall ye boast yourselves.  7 Instead of your shame ye shall have double; and instead of dishonor they shall rejoice in their portion: therefore in their land they shall possess double; everlasting joy shall be unto them. 8 For I, Jehovah, love justice, I hate robbery with iniquity; and I will give them their recompense in truth, and I will make an everlasting covenant with them. 9 And their seed shall be known among the nations, and their offspring among the peoples; all that see them shall acknowledge them, that they are the seed which Jehovah hath blessed.  10 I will greatly rejoice in Jehovah, my soul shall be joyful in my God; for he hath clothed me with the garments of salvation, he hath covered me with the robe of righteousness, as a bridegroom decketh himself with a garland, and as a bride adorneth herself with her jewels. 11 For as the earth bringeth forth its bud, and as the garden causeth the things that are sown in it to spring forth; so the Lord Jehovah will cause righteousness and praise to spring forth before all the nations. Deu 30:1-10 And it shall come to pass, when all these things are come upon thee, the blessing and the curse, which I have set before thee, and thou shalt call them to mind among all the nations, whither Jehovah thy God hath driven thee, 2 and shalt return unto Jehovah thy God, and shalt obey his voice according to all that I command thee this day, thou and thy children,, with all thy heart, and with all thy soul; 3 that then Jehovah thy God will turn thy captivity, and have compassion upon thee, and will return and gather thee from all the peoples, whither Jehovah thy God hath scattered thee. 4 If any of thine outcasts be in the uttermost parts of heaven, from thence will Jehovah thy God gather thee, and from thence will he fetch thee: 5 and Jehovah thy God will bring thee into the land which thy fathers possessed, and thou shalt possess it; and he will do thee good, and multiply thee above thy fathers.  6 And Jehovah thy God will circumcise thy heart, and the heart of thy seed, to love Jehovah thy God with all thy heart, and with all thy soul, that thou mayest live. 7 And Jehovah thy God will put all these curses upon thine enemies, and on them that hate thee, that persecuted thee. 8 And thou shalt return and obey the voice of Jehovah, and do all his commandments which I command thee this day. 9 And Jehovah thy God will make thee plenteous in all the work of thy hand, in the fruit of thy body, and in the fruit of thy cattle, and in the fruit of thy ground, for good: for Jehovah will again rejoice over thee for good, as he rejoiced over thy fathers; 10 if thou shalt obey the voice of Jehovah thy God, to keep his commandments and his statutes which are written in this book of the law; if thou turn unto Jehovah thy God with all thy heart, and with all thy soul. Hos 6:1-3 Come, and let us return unto Jehovah; for he hath torn, and he will heal us; he hath smitten, and he will bind us up. 2 After two days will he revive us: on the third day he will raise us up, and we shall live before him. 3 And let us know, let us follow on to know Jehovah: his going forth is sure as the morning; and he will come unto us as the rain, as the latter rain that watereth the earth. And great revival and restoration will come. Eze 37:1-10 The hand of Jehovah was upon me, and he brought me out in the Spirit of Jehovah, and set me down in the midst of the valley; and it was full of bones. 2 And he caused me to pass by them round about: and, behold, there were very many in the open valley; and, lo, they were very dry. 3 And he said unto me, Son of man, can these bones live? And I answered, O Lord Jehovah, thou knowest. 4 Again he said unto me, Prophesy over these bones, and say unto them, O ye dry bones, hear the word of Jehovah. 5 Thus saith the Lord Jehovah unto these bones: Behold, I will cause breath (Spirit) to enter into you, and ye shall live. 6 And I will lay sinews upon you, and will bring up flesh upon you, and cover you with skin, and put breath (Spirit) in you, and ye shall live; and ye shall know that I am Jehovah.  7 So I prophesied as I was commanded: and as I prophesied, there was a noise, and, behold, an earthquake; and the bones came together, bone to its bone. 8 And I beheld, and, lo, there were sinews upon them, and flesh came up, and skin covered them above; but there was no breath in them. 9 Then said he unto me, Prophesy unto the wind, prophesy, son of man, and say to the wind, Thus saith the Lord Jehovah: Come from the four winds, O breath, and breathe (Spirit) upon these slain, that they may live. 10 So I prophesied as he commanded me, and the breath came into them, and they lived, and stood up upon their feet, an exceeding great army. Isa 2:1-3 The word that Isaiah the son of Amoz saw concerning Judah and Jerusalem. 2 And it shall come to pass in the latter days, that the mountain of Jehovah's house shall be established on the top of the mountains, and shall be exalted above the hills; and all nations shall flow unto it. 3 And many peoples shall go and say, Come ye, and let us go up to the mountain of Jehovah, to the house of the God of Jacob; and he will teach us of his ways, and we will walk in his paths: for out of Zion shall go forth the law, and the word of Jehovah from Jerusalem. Isa 49:13-23 Sing, O heavens; and be joyful, O earth; and break forth into singing, O mountains: for Jehovah hath comforted his people, and will have compassion upon his afflicted. 14 But Zion said, Jehovah hath forsaken me, and the Lord hath forgotten me. 15 Can a woman forget her sucking child, that she should not have compassion on the son of her womb? yea, these may forget, yet will not I forget thee. 16 Behold, I have graven thee upon the palms of my hands; thy walls are continually before me. 17 Thy children make haste; thy destroyers and they that made thee waste shall go forth from thee. The faction is separated from the Bride. 18 Lift up thine eyes round about, and behold: all these gather themselves together, and come to thee. As I live, saith Jehovah, thou shalt surely clothe thee with them all as with an ornament, and gird thyself with them, like a bride.  19 For, as for thy waste and thy desolate places, and thy land that hath been destroyed, surely now shalt thou be too strait for the inhabitants, and they that swallowed thee up shall be far away. 20 The children of thy bereavement shall yet say in thine ears, The place is too strait for me; give place to me that I may dwell. 21 Then shalt thou say in thy heart, Who hath begotten me these, seeing I have been bereaved of my children, and am solitary, an exile, and wandering to and fro? and who hath brought up these? Behold, I was left alone; t

Preserved From The Beast (25) David Eells - 1-2-22 (Audio) Babylon Weaponizes Cells and Computers   We just received by faith at random a text we have received many, many times. It is the story of God's apostate people trusting in the strength of man to save them from the DS Babylonish Beast, which is costing their lives. Jer. 43:4-7  So Johanan the son of Kareah, and all the captains of the forces, and all the people, obeyed not the voice of Jehovah, to dwell in the land of Judah.  5  But Johanan the son of Kareah, and all the captains of the forces, took all the remnant of Judah, that were returned from all the nations whither they had been driven, to sojourn in the land of Judah;  6  the men, and the women, and the children, and the king's daughters, and every person that Nebuzaradan the captain of the guard had left with Gedaliah the son of Ahikam, the son of Shaphan; and Jeremiah the prophet, and Baruch the son of Neriah.  7  And they came into the land of Egypt; for they obeyed not the voice of Jehovah: and they came unto Tahpanhes.  God's people went to trust in Egypt to save them from Babylon who then went to Egypt and killed them.  Jer 43:9-11  Take great stones in thy hand, and hide them in mortar in the brickwork, which is at the entry of Pharaoh's house in Tahpanhes, in the sight of the men of Judah;  10  and say unto them, Thus saith Jehovah of hosts, the God of Israel: Behold, I will send and take Nebuchadrezzar the king of Babylon, my servant, and will set his throne upon these stones that I have hid; and he shall spread his royal pavilion over them.  11  And he shall come, and shall smite the land of Egypt; such as are for death shall be given to death, and such as are for captivity to captivity, and such as are for the sword to the sword. I thought to myself this must be very important to the Lord since we have received this text many times concerning his people going down to Egypt and being killed by the DS Babylon, which represents those trusting in the DS depopulationists to give them a vaccine rather than trust the Lord. Jer 17:5-8  Thus saith Jehovah: Cursed is the man that trusteth in man, and maketh flesh his arm, and whose heart departeth from Jehovah.  6  For he shall be like the heath in the desert, and shall not see when good cometh, but shall inhabit the parched places in the wilderness, a salt land and not inhabited.  7  Blessed is the man that trusteth in Jehovah, and whose trust Jehovah is.  8  For he shall be as a tree planted by the waters, that spreadeth out its roots by the river, and shall not fear when heat cometh, but its leaf shall be green; and shall not be careful in the year of drought, neither shall cease from yielding fruit. DS Babylon just burned a thousand homes in Colorado just like in Paradise with D/E/W weapons. Conservatives and Christians were resisting the DS there. It reminds me of this verse about Babylon taking down the apostate leadership of God's people. Jer 52:13  And he burned the house of Jehovah (Churches), and the king's house (Their leaderships houses); and all the houses of Jerusalem, even every great house (The great men), burned he with fire. Based on these verses I said almost 5 years ago that Babylon would burn churches and leadership homes. Many apostate Church leaders have sent their people to get the vac/cin/es and now they are dying. Backlash is coming.   In the US, the Supreme Court has ruled that vaccinated people worldwide are products, patented goods, according to US law, no longer human. Telegram @ Dark Universe Through a modified DNA or RNA vaccination, the mRNA vaccination, the person ceases to be human and becomes the OWNER of the holder of the modified GEN vaccination patent, because they have their own genome and are no longer “human” (without natural people), but “trans-human”, so a category that does not exist in Human Rights. The quality of a natural person and all related rights are lost. This applies worldwide and patents are subject to US law. Since 2013, all people vaccinated with GM-modified mRNAs are legally trans-human and legally identified as trans-human and do not enjoy any human or other rights of a state, and this applies worldwide, because GEN-POINT technology patents are under US jurisdiction and law, where they were registered. Download PDF here: Vaccined No Longer legally human .pdfDownload SOURCE of the decision of the US SUPREME COURT    Then this came to me that Michael had found a video that the DS is able to assassinate people by their cellphone. And also the Grap/hine ox/ide computer/cellphone connection is in those who were vac/cin/ated.  The thought came to me that they are able to execute these people by cell phone or computer which we know is capable to contact the graphine oxide in the vaccinated to affect their person. This could be done on multitudes of people scattered over a large area and people would just consider that they died of Covid and the educated would believe by the vac/cin/e.   Here is the video of a woman who is getting her nails done at a salon. The salon video cameras picked up footage of her cell phone sending out electrical flashes towards her left arm and then she immediately went into convulsions and fell out onto the floor.    One Day In 2022 Your Cell Phone May Kill You Or Turn You Into Zombies Before it's News - 12/26/21   Did they figure out how to depopulate humanity using frequency sound weapons, using cell phone tech? I'm not sure they would even need 5G to do that but I do believe that's the plan!! Remember that 2022 is the final year in their three year plan... Frequency weapon…. some of us have seen the videos where people are being frequency attacked by their phones… that's why this video is important. It's likely the woman had the vaxx… did she live or die on the spot?  Your Cell Phone May Kill You    1980′s Music Video “Experiment IV” About Frequency Sound Weapon   Kate Bush nails it on 1980′s Music Video “Experiment IV” About Frequency Sound Weapons… [Editor's note: This video appears to show an inventor whose heart was to do good to humanity with his invention. I believe this could represent Nicolai Tesla whose revelation and inventions proved that frequencies could heal which was his purpose, however, wicked military people turned this into a weapon on the other end of the scale of frequencies. In the video it appears he was very frustrated and angry this was happening. It appeared to at first be a good thing but ended up being deadly. When it got out in the video it killed many.] The final chilling words of her song, “And the public are warned to stay off.” Here are the lyrics:  [Verse 1] We were working secretly for the military. Our experiment in sound was nearly ready to begin. We only know in theory what we are doing. Music made for pleasure, music made to thrill. It was music we were making here, until…[Chorus] They told us all they wanted was a sound that could kill someone from a distance. So we go ahead, and the meters are over in the red. It's a mistake in the making.  [Verse 2] From the painful cry of mothers. To the terrifying scream. We recorded it and put it into our machine. [Chorus] But they told us all they wanted was a sound that could kill someone from a distance. So we go ahead, and the meters are over in the red. It's a mistake that we're making.  [Bridge] It could feel like falling in love. It could feel so bad. But it could feel so good. It could sing you to sleep. But that dream is your enemy. [Verse 3] We won't be there to be blamed. We won't be there to snitch. (The killers will be far away)  I just pray that someone there can hit the switch. [Editor's note: It appears that Tesla tried to turn the switch off but failed.] [Chorus] But they told us all they wanted was a sound that could kill someone from a distance. So we go ahead, and the meters were over in the red. It's a mistake we've made. [Outro] And the public are warned to stay off.    Weaponized Cell Phone Theory Demonstrated in the Movie “Cell”  [Editor's note: John Cusack and Samuel L. Jackson star in this tale of a mysterious phone signal that turns much of the population into murderous predators. Signature Entertainment releases Cell. [Here is the trailer: https://youtu.be/BCns4w3GA9A ]   Zombie Alert Early 2022 Watch the Movie “Cell” Now… It Is Probably A Documentary!!  [Editor's note: The DS always tells us what they are going to do before they do it.]  (Writer's) Theory: Zombies Created With 5G + Graphene Oxide (Vaxx Ingredient) + Cell Phone!! CONOP 8888 US Dept. Of Defense Strategic Command Zombie Defense Plan Exists!! Airlines Panicking Over 5G Rollout!! Folks… this is important… there IS a zombie event coming… the military AND Pentagon already know!!   U.S. military actually made a zombie apocalypse training manual. Yes, really. Internet Culture Published May 14, 2014 - Updated May 31, 2021  If the U.S. is ever attacked by evil magic zombies, or even the relatively tame creature that is a chicken zombie, rest assured that the Department of Defense has it covered. Seriously, a document has apparently been unearthed by Foreign Policy magazine that appears to show either the incredible level of preparedness of the U.S. national security community, or it demonstrates how the people tasked with keeping the nation safe are insane. The zombie attack training manual, “CONOP 8888,” or “Counter Zombie Dominance,” is dated April 2011, and was supposedly found buried on a secret military computer. It tells military planners how to defeat an invasion of flesh eaters, (Editor's note: Remember he outbreak of zombies that were devouring peoples faces.) and how to preserve the sanctity of “non-zombie life.” From magic zombies to chicken zombies, even vegetarian zombies, they're all in the file, with a plan for how to take them out.  “Zombies pose a threat to all non-zombie human life,” says CONOP8888. “[Strategic Command] will be prepared to preserve the sanctity of human life and conduct operations in support of any human population—including traditional adversaries.” While the manual says in its disclaimer that “this plan was not acutally designed as a joke,” it still kind of is. What the intelligence community has done here is use “zombie” as a kind of code word for “enemy,” so as not to look like the Pentagon is planning for an invasion of a particular country, religion, or plague. To prepare for all possible situations, it says, “We elected to use a completely-impossible scenario that could never be mistaken for a real plan.” All of which begs the question, of course, if zombies are being used as metaphors for real threats, what... are “Vegetarian zombies” doing in there? Chicken zombies could, of course, mean avian bird flu, and evil magic zombies could easily be a thinly veiled reference to Al' Qaeda or some other real enemy. But there can be no such thing as a vegetarian zombie. And second, what threat do vegetarians pose, either in this universe or the one inhabited by the person who wrote this manual? In fact, according to the military zombie survival guide, vegetarian zombies “pose no direct threat to humans because they only eat plant life,” which makes total sense… CONOP 8888 also known as Counter-Zombie Dominance is a U.S. Department of Defense Strategic Command CONOP document that describes a plan for defending against zombies. The April 30, 2011 document depicts fictional scenarios of zombie attacks for training students in military planning. Read the full zombie apocalypse training manual below: CONPLAN 8888 Now if that was all bad enough… the airlines are panicking over 5G (The opening scene of the movie “Cell” takes place in airport!!)  What do they know????    Planned 5G Wireless Deployment Could Compromise Airline Safety, Aviation Executives Warn  Newsweek - Justin Klawans - Dec 21, 2021    Aviation executives expressed their fears Monday that the potential coming deployment of 5G wireless technology could harm the airline industry. In a joint letter acquired by Reuters addressed to the U.S. Department of Transportation (DOT), Boeing CEO Dave Calhoun and Airbus Americas CEO Jeffrey Knittel urged DOT Secretary Pete Buttigieg to postpone the upcoming rollout of the technology, citing multiple concerns. "5G interference could adversely affect the ability of aircraft to safely operate," the letter stated, adding that the rollout of the technology could have "an enormous negative impact on the aviation industry.” Both AT&T and Verizon are planning to deploy C-band spectrum 5G wireless networks across the United States on Jan. 5. The technology was originally slated to go online in November, but the companies agreed to push the date into 2022 after concerns were cited, and now the airline executives are hoping to slide that date even further down the calendar. The airplane manufacturers, who are typically considered direct competitors, came together in a rare show of unity to voice concerns that the 5G initiative could lead to numerous delays at airports throughout the country. The pair of companies represent the vast majority of the airplanes built for commercial aviation use, and they also have a large market share of military aircraft. The letter from Boeing and Airbus cited a statistic from trade group Airlines for America, which stated that if 5G had been in effect in 2019, it would've resulted in the delay or cancellation of 345,000 passenger flights and 5,400 cargo flights. The sentiment from the airline executives echoed a report released Dec. 7 from the U.S. Federal Aviation Administration (FAA) that said 5G technology could lead to scheduling problems and safety issues in the skies. After issuing its report, the FAA went on to present a pair of airworthiness directives that would prohibit aircraft from operating certain radio functions while in the area of 5G signals. "Because radio altimeter anomalies that are undetected by the aircraft automation or pilot, particularly close to the ground ... could lead to loss of continued safe flight and landing," one of the directives stated. However, the FAA later issued a statement in which it said that the "expansion of 5G and aviation will safely co-exist." Verizon also released a statement pushing back against the FAA guidelines, stating that "there is no evidence that 5G operations using C-band spectrum pose any risk to aviation safety, as the real-world experience in dozens of countries already using this spectrum for 5G confirms." The communications giant added that it was taking "additional steps to minimize energy coming from 5G base stations." However, in spite of the statements released by multiple parties, the Air Line Pilots Association stated Monday that the discussions regarding 5G were at a stalemate, adding that it was "a big problem for passengers, shippers and the American economy.”  Equally important to look at is what is in the vaccines: Look At The Freaky Live Blood Sample Of Vaxxed Under Microscope!! “The Thing” Is Definitely Alive, As Are Other Strange Unidentified Creatures Swimming Around In Blood Sample Of Vaxxed!! It's Like An Ecosystem Humanity Has Never Seen Before!!     Dr. Ariyana Love said, "I agree with what one scientist suggested… that the reason the vaxxes are kept under extremely cold storage, is to keep the creatures under a suspended animation… until they are thawed out, and activated by human temperatures.  Now looking at this blood sample.. it's clear we can see several creatures moving about, a blue colourful ‘thing', and a large ‘hydra'-like creature, that some are suggesting might be able to self-regenerate itself, and therefore is basically, immortal.  The tinier critters look rather ‘active' and like they are more developed than simple bacteria… I wonder what everybody is doing… probably eating stuff, eliminating waste and reproducing. Most likely… what this means, is that the blood of the vaxxed has likely become a literal alien-hybrid cesspool of waste byproducts, and who knows what else… we're still finding out! Folks… we could be looking at an alien invasion under microscope… and at the very least, the new blood of the new hybrid (human)!”    Is This Why They Want to Change our DNA?  Source: Uncensored Sam, Published September 9, 2021  God's true name is recorded within our DNA. So, just let me go ahead and explain. Our chains of DNA contain four nucleotide-based pairs called Adenine, Guanine, Thiamine, and Cytosine. And then there's a unit within the DNA strand called a sulfuric bridge.  This sulfuric bridge holds our entire DNA strand together. It only appears after every ten nucleic acids have been laid down, and then after every five nucleic acids, then after every six, and then again, after every five nucleic acids. So, we get this repeating numerical value of 10, 5, 6, 5 within our DNA strand. Well, it turns out the Biblical name for God, YHWH, contains the same numerical value as our DNA strand. Yod He Vav He equals ten, five, six, five. What's even more interesting is that if you take the Hebrew name of YHWH and place it on its side, you get the image of a human being. Part three of this video series suggests that DNA is a language. Many people were wondering what god made the language of DNA. Researchers at Harvard University announced today that they have found what appears to be a message from God written inside the human genome. In a little-explored section of non-coding DNA, a team of top geneticists discovered a 22-word snippet of ancient Aramaic in which God confirms this existence and His role in creating life on Earth. “We knew the patterns weren't naturally occurring,” explains Charles Watson, the lead scientist on the project, “but we couldn't come up with any convincing explanation for them.  On a whim, we started cross-referencing the patterns with language databases,” he explains, “and we were shocked to find that the patterns corresponded to ancient Aramaic.” Stunned by its discovery, the team contacted language experts familiar with Aramaic – the language Jesus Christ spoke in daily life. Entirely decoded, the message reads: Hello my children, This is Yahweh, the One True Lord. You have found creation's secret. Now…go tell the world Who is the true God and Creator of all! Rumble Video: IS THIS Why They Want to Change our DNA?  Covid Shots Turn Humanity Into Patentable Species!! Supreme Court Ruling, And It's In The Patents!! Covid Shots Turn Humanity Into Patentable Species!! Supreme Court Ruling, And It's In The Patents!! Zombie Invasion Begins Mid-January 2022… Along With Mass Sickness And Death Especially For The Jabbed.     Zombie Invasion Begins Mid-January 2022… Along With Mass Sickness And Death Especially For The Jabbed!! Make The Omicron/Zombie/Disease X Connection Now And Then Head For The Hills!! One News - December 23, 2021   People… what we have here, is proof, that there is collusion and conspiracy for mass murder, and zombification of humanity on a scale that defies most people's awareness. For starters you must understand that new technologies especially of the electromagnetic radiation kind, tend to coincide with new illnesses. This has been going on for DECADES. Now… the latest is the 5G rollout… a major step scheduled for early January. I'd like you to simply observe the mass media…. two things are ‘scheduled: 1.  5G rollout… a new wave of 5G tech is set to become active.  https://duckduckgo.com/?q=++5g+rollout+january&atb=v255-1&iar=news&ia=news 2.  Media keeps suggesting people will get sick early January! https://duckduckgo.com/?q=+january+new+variant&atb=v255-1&iar=news&ia=news 3.  They seem to know something about ‘Disease X' aka 5G radiation sickness. https://duckduckgo.com/?q=disease+x&atb=v255-1&iar=news&ia=news Gee how do they know? They know because they know 5G is going to make people sick… and even worse. They know the graphene oxide in the vaxxed, is activated by electromagnetic radiation. This Is What Will Create Zombies!! Watch Graphene Oxide Dance To A Frequency In A Lab!! What This Means Is That Vaxx Ingredient Can Be Frequency Adjusted To Do… They know graphene oxide at the molecular level is equivalent of tiny razor blades; a leading scientist was murdered for telling the world this FACT! If you have the jab or several… you probably have lots to be concerned about with 5G. [Editor's note: Fear not… Lo, I will be with you always. From here on down he is speaking to non-believers.] If I were you… I'd pretty much be heading to the countryside. [Editor's note: Since cities are where most of the sin happens and most of the judgment comes, I always recommend people move out of the cities and away from the fault lines and sea shores.] How are you going to live, when tiny razor blades are slicing through your body… 24/7? [Editor's note: Those who believe the Word of God will live by Psalm 91, where it says, “There shall no evil befall thee." and the Passover, which is where God exercised control over the destroyer for those who lived under the blood. Jesus said in Luk 10:19  Behold, I have given you authority to tread upon serpents and scorpions, and over all the power of the enemy: and nothing shall in any wise hurt you.] How are you going to be healthy? How are you going to survive? [Learn from God's Vaccine Book] How will your body repair itself, as all the organs and capillaries are receiving 1000's of tiny slices… non-stop! [Remember, "nothing shall in any wise hurt you."] 5G is bad enough if you are not jabbed, but if you are, it might be a death sentence, or at the very least, the end of your body as you know it. [Gal 3:13 Christ redeemed us from the curse of the law, having become a curse for us; for it is written, Cursed is every one that hangeth on a tree:] Face it… what if graphene oxide is in the brain, and then you expose yourself to that radiation that is 100x stronger than 4G? [Pro 10:2  Treasures of wickedness profit nothing; But righteousness delivereth from death.] Now… this is also a trend in the news… the airlines are already bracing people for weirdness. Imagine a vaxxed pilot exposed constantly to 5G… to me, it's like a scenario of the rise of the zombies. Their brains will be sliced over and over by tiny razor blades one molecule thick. This will surely be the rise of the zombies. This sounds like a big joke right?  MILITARY DOES NOT THINK SO and they are also EXPERTS IN ELECTROMAGNETIC WARFARE! Hundreds of military, law enforcement and medical personnel will observe the Hollywood-style production of a zombie attack as part of their emergency response training.  We've put some links below: https://duckduckgo.com/?q=military+zombie+training&atb=v255-1&ia=web Marines, police prep for mock zombie invasion | Fox News https://www.foxnews.com › us › marines-police-prep-for-mock-zombie-invasion You need to know that they don't have a real accurate test for omicron… or the other variants, or covid. That's why they are ‘phasing out' the PCR test… they use this term ‘phase out' instead of ‘it never worked' to avoid admission of guilt for crimes against humanity… or let's be blunt, this is the greatest crime against humanity that has ever been perpetrated, and they won't stop unless humanity figures things out and stops them…  GRAPHENE OXIDE AND THE END-OF-DAYS APOCALYPSE Posted on August 12, 2021 by State of the Nation  Dr. José Luis Sevillano expresses his theory that GRAPHENE OXIDE (GO) is the Bio-Tech ingredient chosen for controlling mankind in THE NEW WORLD ORDER as defined by the DAVOS, the Bildebergs and WWII NAZI collaborator, Klaus Schwab, who founded the Word Economic Forum.  Graphene Oxide (GO) is found in the current Covid-19 vaccines.  GO is a super- conductor with unique electromagnetic properties that can facilitate the altering of human genetics.  The Covid-19 mRNA vaccines are the trial run for the “genetic Apocalypse”.  The plan is to alter the human species to install population control and coercive slavery through injection of Graphene Oxide into the blood stream where intra-cellular conductivity will allow neura-link (Elon Musk) nano technology and remote control of the human body and brain using directed electromagnetic radiation of various frequencies… https://www.orwell.city/2021/08/graphene-and-reset.html They are desperate to get this Graphene Oxide into people any way they can including, Spraying it in the Air, putting it in food and the nasal swabs for the PCR tests.   Sep 23, 2021 - RED ALERT: Aerosolized skin-penetrating nanoparticle spike protein attack vector CONFIRMED https://www.brighteon.com/358dc7fe-2580-40c6-8a90-13a13175caf5 Operation Disclosure: "Former FEMA Whistleblower Celeste Solum explains that the Corona PCR “test” is implanting a microchip  So the Nasopharyngeal Covid19 PCR “tests” were never tests at all. They are implanting chips, inserting Nanobots called Nanites with a bioweapons payload for the brain, while at the same time harvesting DNA. The PCR swab is inserted straight to the Nasopharynx behind the nose and forehead where your eyes are. It's at the blood brain barrier and next to your pineal gland. I believe they are knocking out people's senses with these Bioweapons and killing human intuition and your ability to cognitively see what they are doing to us. They're knocking out our senses and targeting the brain with Nanites that carry a payload. Decline the “tests.” Refuse the masks. Don't let them implant the microchip.”  Here are some very informative links explaining Graphene Oxide; It's history and what they plan on using it for: https://www.holistichealthonline.info/graphene-oxide-basics/ https://www.ftwproject.com/ref/6/      GRAPHENE OXIDE, 5G AND COVID PRESENTATION AND NOTES ON SGT REPORT  Here is a quote from Klaus Schwab's book, “Covid - 19: The Great Reset: “Euthanasia for the terminally ill and the aged shall be compulsory. No cities shall be larger than a predetermined number as described in the work of Kalgeri. Essential workers will be moved to other cities if the one they are in becomes overpopulated. Other non-essential workers will be chosen at random and sent to underpopulated cities to fill "quotas”. At least 4 billion “useless eaters” shall be eliminated by the year 2050 by means of limited wars, organized epidemics of fatal rapid-acting diseases and starvation. Energy, food and water shall be kept at subsistence levels for the non-elite, starting with the White populations of Western Europe and North America and then spreading to other races. The populations of Canada, Western Europe and the United States will be decimated more rapidly than on other continents, until the world's population reaches a manageable level of 1 billion, of which 500 million will consist of Chinese and Japanese races, selected because they are people who have been regimented for centuries and who are accustomed to obeying authority without question. From time to time there shall be artificially contrived food and water shortages and medical care to remind the masses that their very existence depends on the good will of the Committee of 300.”    The Culling of the Human Race Part 2  BY GERI UNGUREAN - 11/21/20 Altering DNA – Luciferase Biogel – Implantable Biochip to be Tracked by 5G Technology- It Isn't a Conspiracy Theory When They Are Openly Telling You What They Want To Do First and foremost, I encourage the reader to watch this short video  in which Dr. Carrie Madej explains the mRNA Vaccine in terms that we can understand. She is a brave woman to openly expose the dangerous, DNA-changing vaccine.   DARPA funded implantable biochip can potentially deploy Moderna's mRNA vaccine   From nanotechnology.news In a bid to try to battle the ongoing Wuhan coronavirus (COVID-10) pandemic, the Defense Advanced Research Projects Agency (DARPA) is funding the development of an implantable biochip that could be deployed as soon as next year. The chip is said to be able to deploy an experimental new vaccine, developed jointly by Moderna and U.S government, that could change human DNA. The chip and the vaccine built on a breakthrough made by then-Harvard University professor and eventual Moderna co-founder Derek Rossi in 2010. In his discovery, which the now-retired scientist says came about purely by accident, Rossi claimed that he found a way to “reprogram” messenger ribonucleic acid (mRNA) – the molecules that carry genetic instructions for cell development in the human body. The promise provided by Rossi's breakthrough was such that it allowed him to co-found Moderna with private equity firm Flagship Pioneering. The company then attracted almost half a billion dollars from the federal government to begin developing vaccines using the new technology. In a preliminary report last July funded by the National Institute of Allergy and Infectious Diseases (NIAID) and the National Institutes of Health (NIH), Moderna's mRNA-1273 vaccine was found to have “induced anti–SARS-CoV-2 immune responses in all participants and no trial-limiting safety concerns were identified.” Despite this, however, one obstacle to the deployment of Moderna's vaccine is the method of delivery. While Moderna is developing its own system, it's unlikely to get Food and Drug Administration (FDA) approval any time soon. Enter Profusa, which is developing a nanoscale biochip that can detect symptoms of an infection. Profusa's biochip is made using a technology called “hydrogels” that were a product of the “In Vivo Nanoplatforms” (IVN) program that DARPA's Biological Technologies Office (BTO) launched in 2014 to develop implantable nanotechnologies. These hydrogels are soft, flexible nanomachines that are injected beneath the skin to perform monitoring. This hydrogel includes a specially engineered molecule that sends a fluorescent signal outside the body when it begins to fight infection. This signal can then be detected by a sensor attached to the skin that can then be sent to an app or even to a doctor's website. When it was first tested in 2018, this hydrogel was being used to measure glucose, oxygen and lactate levels. However, this past March, the company quietly announced that it was conducting a study to see if the technology could be used to detect respiratory diseases, including COVID-19. More importantly, Profusa's biochip is expected to receive fast-track authorization from the FDA by early 2021. With this in mind, some are speculating that the biochip will more than likely be the candidate for the mRNA vaccine's delivery. Read rest of article HERE    From mintpress.com The most significant scientific discovery since gravity has been hiding in plain sight for nearly a decade and its destructive potential to humanity is so enormous that the biggest war machine on the planet immediately deployed its vast resources to possess and control it, financing its research and development through agencies like the National Institutes of Health (NIH), the Defense Advanced Research Projects Agency (DARPA) and HHS' BARDA. The revolutionary breakthrough came to a Canadian scientist named Derek Rossi in 2010 purely by accident. The now-retired Harvard professor claimed in an interview with the National Post that he found a way to “reprogram” the molecules that carry the genetic instructions for cell development in the human body, not to mention all biological lifeforms. These molecules are called ‘messenger ribonucleic acid' or mRNA and the newfound ability to rewrite those instructions to produce any kind of cell within a biological organism has radically changed the course of Western medicine and science, even if no one has really noticed yet. As Rossi, himself, puts it: “The real important discovery here was you could now use mRNA, and if you got it into the cells, then you could get the mRNA to express any protein in the cells, and this was the big thing.” It was so big that by 2014, Rossi was able to retire after the company he co-founded with Flagship Pioneering private equity firm to exploit his innovation, – Moderna Inc., attracted almost a half billion dollars in federal award monies to begin developing vaccines using the technology. No longer affiliated with Moderna beyond his stock holdings, Rossi is just “watching for what happens next” and if he's anything like the doting “hockey dad” he is portrayed to be, he must be horrified. Remote control biology As early as 2006, DARPA was already researching how to identify viral, upper respiratory pathogens through its Predicting Health and Disease (PHD) program, which led to the creation of the agency's Biological Technologies Office (BTO), as reported by Whitney Webb in a May article for The Last American Vagabond. In 2014, DARPA's BTO launched its “In Vivo Nanoplatforms” (IVN) program, which researches implantable nanotechnologies, leading to the development of ‘hydrogel'. Hydrogel is a nanotechnology whose inventor early on boasted that “If [it] pans out, with approval from FDA, then consumers could get the sensors implanted in their core to measure their levels of glucose, oxygen, and lactate.” This contact lens-like material requires a special injector to be introduced under the skin where it can transmit light-based digital signals through a wireless network like 5G. Once firmly implanted inside the body, human cells are at the mercy of any mRNA program delivered via this substrate, unleashing a nightmare of possibilities. It is, perhaps, the first true step towards full-on transhumanism; a “philosophy” that is in vogue with many powerful and influential people, such as Google's Ray Kurzweil and Eric Schmidt and whose proponents see the fusion of technology and biology as an inevitable consequence of human progress. The private company created to market this technology, that allows for biological processes to be controlled remotely and opens the door to the potential manipulation of our biological responses and, ultimately, our entire existence, is called Profusa Inc and its operations are funded with millions from NIH and DARPA. In March, the company was quietly inserted into the crowded COVID-19 bazaar in March 2020, when it announced an injectable biochip for the detection of viral respiratory diseases, including COVID-19. Read rest of article HERE This next article REALLY caught my attention. These bad actors on the world stage are not even trying to hide anything from the public. I believe that this has happened because of the FEAR TACTIC. Many people who have given up their liberties and have cloistered themselves in their homes are TERRIFIED. Klaus Schwab is the head of the WEF (World Economic Forum). This man is salivating over the coming New World Order.   Klaus Schwab: Great Reset Will “Lead to a Fusion of Our Physical, Digital and Biological Identity” From summitnews.com Globalist Klaus Schwab made it clear that transhumanism is an integral part of “The Great Reset” when he said that the fourth industrial revolution would “lead to a fusion of our physical, digital and biological identity,” which in his book he clarifies is implantable microchips that can read your thoughts. As we highlighted earlier, “The Great Reset” is attracting a deluge of fresh attention in the aftermath of the coronavirus pandemic, which Canadian Prime Minister Justin Trudeau said was an “opportunity for a reset.” The agenda is primarily based around dismantling the current capitalist system in favor of greater centralized technocrat rule which will lead to lower living standards, less fuel consumption, fewer civil liberties and the accelerated automation of jobs. However, another key aspect to “The Great Reset,” or the “fourth industrial revolution” as Schwab calls it, is merging man with machine. #TheGreatReset #4IR https://t.co/1ZjrP7F8RQ Schwab went on to explain how his book, ‘Shaping the Future of The Fourth Industrial Revolution', was particularly popular in China, South Korea and Japan, with the South Korean military alone purchasing 16,000 copies. In the book, Schwab explains with excitement how upcoming technology will allow authorities to “intrude into the hitherto private space of our minds, reading our thoughts and influencing our behavior.” He goes on to predict that this will provide an incentive for law enforcement to implement Minority Report-style pre-crime programs. “As capabilities in this area improve, the temptation for law enforcement agencies and courts to use techniques to determine the likelihood of criminal activity, assess guilt or even possibly retrieve memories directly from people's brains will increase,” writes Schwab. “Even crossing a national border might one day involve a detailed brain scan to assess an individual's security risk.” Schwab also waxes lyrical about the transhumanist utopian dream shared by all elitists which will ultimately lead to the creation of human cyborgs. “Fourth Industrial Revolution technologies will not stop at becoming part of the physical world around us—they will become part of us,” writes Schwab.  “Indeed, some of us already feel that our smartphones have become an extension of ourselves. Today's external devices—from wearable computers to virtual reality headsets—will almost certainly become implantable in our bodies and brains.” Schwab also openly endorses something the media still claims is solely a domain of discussion for conspiracy theorists, namely “active implantable microchips that break the skin barrier of our bodies.” The globalist hails the arrival of “implanted devices (that) will likely also help to communicate thoughts normally expressed verbally through a ‘built-in' smartphone, and potentially unexpressed thoughts or moods by reading brain waves and other signals.” So in other words, the “fusion of our physical, digital and biological identity” relates to the transhumanist singularity and a future where people have their every movement tracked and every thought read by an implantable microchip. It isn't a “conspiracy theory” when they're openly telling you what they want to do. source I want to say something very important to the brethren. The Cov-19 vaccine is NOT the Mark of the beast! … [Editor's note: This technology now is not the Mark of the Beast because this starts in the middle of the tribulation. So here is the mid-trib Mark of the Beast.] Rev. 13:11-18  “Then I saw another beast coming up out of the earth, and he had two horns like a lamb and spoke like a dragon.  And he exercises all the authority of the first beast in his presence, and causes the earth and those who dwell in it to worship the first beast, whose deadly wound was healed.  He performs great signs, so that he even makes fire come down from heaven on the earth in the sight of men.  And he deceives those who dwell on the earth by those signs which he was granted to do in the sight of the beast, telling those who dwell on the earth to make an image to the beast who was wounded by the sword and lived.  He was granted power to give breath to the image of the beast, that the image of the beast should both speak and cause as many as would not worship the image of the beast to be killed.  He causes all, both small and great, rich and poor, free and slave, to receive a mark on their right hand or on their foreheads, and that no one may buy or sell except one who has the mark or the name of the beast, or the number of his name. Here is wisdom. Let him who has understanding calculate the number of the beast, for it is the number of a man: His number is 666... Those on the earth during this time who come to the realization that Christ is Lord – they will refuse to take the mark. They will also be hunted down by the armies of Antichrist and will be beheaded. But their souls will be in heaven with our Precious Lord Jesus. Remember this passage of Scripture?  Rev. 6:10 And they cried with a loud voice, saying, “How long, O Lord, holy and true, until You judge and avenge our blood on those who dwell on the earth?”   These were those who were slaughtered for their faith by Antichrist. They are asking the Lord “How long before He will judge and avenge their blood?” Brethren, I hope that this has helped some who worry about the vaccine being the actual mark of the beast. But I still say DO NOT GET THE VACCINE!! It changes our DNA which is God-given! Pray and share the saving Gospel of Jesus Christ with the lost. Time is short!   Conquering Your Beast Holiness or sanctification is a refuge from the Beast as a highway of holiness that leads to Zion. The unclean cannot travel this highway. “Righteousness delivereth from death.” The Beast is always given authority to crucify the flesh. If a person is dead to self, which is a gift from God, they never have to worry about the Beast. Isa 35:1-10  The wilderness (Tribulation in Revelation 12 & 17) and the dry land shall be glad; and the desert shall rejoice, and blossom as the rose.  2  It shall blossom abundantly, and rejoice even with joy and singing; the glory of Lebanon shall be given unto it, the excellency of Carmel and Sharon: they shall see the glory of Jehovah, the excellency of our God.  3  Strengthen ye the weak hands, and confirm the feeble knees.  4  Say to them that are of a fearful heart, Be strong, fear not: behold, your God will come with vengeance, with the recompense of God; he will come and save you.  5  Then the eyes of the blind shall be opened, and the ears of the deaf shall be unstopped.  6  Then shall the lame man leap as a hart, and the tongue of the dumb shall sing; for in the wilderness shall waters break out, and streams in the desert.  7  And the glowing sand shall become a pool, and the thirsty ground springs of water: in the habitation of jackals, where they lay, shall be grass with reeds and rushes.  8  And a highway shall be there, and a way, and it shall be called The way of holiness; the unclean shall not pass over it; but is shall be for the redeemed: the wayfaring men, yea fools, shall not err therein.  9  No lion shall be there, nor shall any ravenous beast go up thereon; they shall not be found there; but the redeemed shall walk there:  10  and the ransomed of Jehovah shall return, and come with singing unto Zion; and everlasting joy shall be upon their heads: they shall obtain gladness and joy, and sorrow and sighing shall flee away. Luk 21:34-36  But take heed to yourselves, lest haply your hearts be overcharged with surfeiting, and drunkenness, and cares of this life, and that day come on you suddenly as a snare:  35  for so shall it come upon all them that dwell on the face of all the earth.  36  But watch ye at every season, making supplication, that ye may prevail to escape all these things that shall come to pass, and to stand before the Son of man. {Rev.12:9} And the great dragon was cast down, the old serpent, he that is called the Devil and Satan, the deceiver of the whole world; he was cast down to the earth, and his angels were cast down with him. {10} And I heard a great voice in heaven, saying, Now is come the salvation, and the power, and the kingdom of our God, and the authority of his Christ: for the accuser of our brethren is cast down, who accuseth them before our God day and night... {13} And when the dragon saw that he was cast down to the earth, he persecuted the woman that brought forth the man [child]. {14} And there were given to the woman the two wings of the great eagle, that she might fly into the wilderness unto her place, where she is nourished for a time, and times, and half a time, from the face of the serpent.  

If there is one rule in biology, it is that there is an exception to every rule. This includes even the basic biochemistry of DNA, which was once thought to be universal. On this episode, host Lauren Richardson and Judy Savitskaya (a16z bio deal team member and synthetic biology expert), discuss the results and implications three related articles co-published in Science, which all advance our understanding of a very unique kind of DNA. If you open any biology text book, it will say that the genetic code is made up of 4 bases: Adenine, Thymine, Cytosine, and Guanine, or ATCG. But, back in 1977, scientists discovered a phage — the technical term a virus that infects bacteria — that encodes its genome in ZTCG. Z is a derivative of A that has an extra amino group tagged on, and while that may sound minor, it changes some of the key properties of DNA. These three new articles seek to understand how Z is made and how it is incorporated into DNA. This is essential information for taking Z from a weird, wild bio story into a practical application. The conversation covers what makes Z different than other bases, what these three articles reveal about the synthesis and polymerization of Z, and how we can use use Z in a wide range of applications, from bio-containment to new therapeutics to DNA storage.The three articles discussed are:"A widespread pathway for substitution of adenine by diaminopurine in phage genomes" by  Yan Zhou, Xuexia Xu, Yifeng Wei, Yu Cheng, Yu Guo, Ivan Khudyakov, Fuli Liu, Ping He, Zhangyue Song, Zhi Li, Yan Gao, Ee Lui Ang, Huimin Zhao, Yan Zhang, and Suwen Zhao"A third purine biosynthetic pathway encoded by aminoadenine-based viral DNA genomes" by Dona Sleiman, Pierre Simon Garcia, Marion Lagune, Jerome Loc’h, Ahmed Haouz, Najwa Taib, Pascal Röthlisberger, Simonetta Gribaldo, Philippe Marlière, and Pierre Alexandre Kaminski"Noncanonical DNA polymerization by aminoadenine-based siphoviruses" by  Valerie Pezo, Faten Jaziri, Pierre-Yves Bourguignon, Dominique Louis, Deborah Jacobs-Sera, Jef Rozenski, Sylvie Pochet, Piet Herdewijn, Graham F. Hatfull, Pierre-Alexandre Kaminski, and Philippe Marliere 

My AP Biology Thoughts  Episode #48Welcome to My AP Biology Thoughts podcast, my name is Pauline and I am your host for episode #48 called Unit #1 Chemistry of Life: Nucleic Acids Structure Function & Examples. Today we will be discussing an overview of nucleic acids in terms of their components, function, and examples. Segment 1: Introduction to Nucleic Acids Structure Function & Examples Nucleic Acids are one of the big macromolecules necessary for life. They are complex organic substances present in living cells whose molecules consist of many nucleotides linked in a long chain. Let's first talk about what makes up nucleic acids. Their monomers are nucleotides which are all made up of a phosphate group, a pentose sugar, and a nitrogenous base as shown in the diagram. The nitrogen atoms in the nitrogenous base are the extra element that differentiates nucleic acids from the three other macromolecules with the abbreviation CHOPN. Through a dehydration reaction, nucleotides link together with a phosphodiester linkage which is between the sugar and phosphate groups. The drawing shows a water molecule taken out to form this a covalent bond. The nucleotides bonded together make up the polymers of nucleic acids which are DNA and RNA. Segment 2: Example of Nucleic Acids Structure FunctionDNA and RNA are the main two examples of nucleic acids. There are major differences as well as similarities between DNA and RNA that are important to know. DNA, also known as deoxyribonucleic acid, is made up of the deoxyribose sugar, is double stranded and arranged in an antiparallel pattern. DNA is always found in the nucleus of eukaryotic cells and contains information for thousands of proteins. Its bases are cytosine, guanine, adenine, and thymine. On the other hand, RNA uses all of these bases except thymine is replaced by uracil. RNA is also only single stranded and contains information for only one protein as it leaves the nucleus after formation. They are similar in their directionality and bonding. Their nucleotides are all linked by covalent bonds and have a 3' to 5' directionality. The linear sequence of nucleotides have ends, defined by the 3' hydroxyl and 5' phosphates of the sugar in the nucleotide. During DNA and RNA synthesis, nucleotides are added to the 3' end of the growing strand, resulting in the formation of a covalent bond between nucleotides.  Since DNA is structured as an antiparallel double helix, each strand runs in the opposite 5' to 3' orientation. This means that adenine nucleotides pair with thymine nucleotides via two hydrogen bonds. Cytosine nucleotides pair with guanine nucleotides by three hydrogen bonds. This is all shown in this diagram where the black boxes are where another nucleotide would be added, and there are more hydrogen bonds highlighted in pink between Guanine and cytosine because of the antiparallel double helix.  Segment 3: Digging Deeper Nucleic Acids Structure Function & ExamplesNucleic acids fit in the greater picture of the unit called Chemistry of Life because of their functions. As mentioned before, they carry information for making proteins which brings back the topic of the central dogma. Through transcription, the information in a strand of DNA is copied into a new molecule of messenger RNA, which is then decoded during the process of translation to build a polypeptide. Humans cannot survive without all nine essential amino acids found in proteins, so nucleic acids are essential for protein synthesis. DNA and RNA also have a hereditary function because it is inherited from parent to offspring, so is responsible for any gene mutations and the genetic biodiversity of populations. Just to highlight just how important nucleic acids are, DNA is the blueprint for life. Thank you for listening to this episode of My AP Biology Thoughts. For more student-ran podcasts and digital content, make sure that you...

Students want things easy. We often make it hard for them. Host Kevin Patton discusses desirable difficulty and contrasts it with undesirable difficulty. Did you know that healthy human cells have little sections of 4-stranded DNA? We can be better in our web meeting skills. And don't forget our new online community of anatomy & physiology faculty! 00:46 | G4 DNA 05:58 | Sponsored by AAA 06:38 | Even More Web Meeting Ideas 18:55 | Sponsored by HAPI 19:55 | Desirable Difficulty 35:35 | Sponsored by HAPS 36:26 | Our New Online Community 39:54 | Staying Connected If you cannot see or activate the audio player click here. Apply for your credential (badge/certificate) for listening to this episode. Please take the anonymous survey: theAPprofessor.org/survey Questions & Feedback: 1-833-LION-DEN (1-833-546-6336) Follow The A&P Professor on Twitter, Facebook, Blogger, Nuzzel, Tumblr, or Instagram! There are no secrets to success. It is the result of preparation, hard work, and learning from failure. (Colin Powell)   G4 DNA 5 minutes Oh, come on! Is there really a quadruple-strand DNA in our normal, healthy cells? Or is that only in space aliens? Or zombies? Quadruple-stranded DNA seen in healthy human cells for the first time (news summary of the discovery) my-ap.us/2RXp7Vt Single-molecule visualization of DNA G-quadruplex formation in live cells (journal article in Nature Chemistry) my-ap.us/2EwXr6O Image: G-quadruplex by Julian Huppert my-ap.us/3i70AIv   Sponsored by AAA 1 minute A searchable transcript for this episode, as well as the captioned audiogram of this episode, are sponsored by the American Association for Anatomy (AAA) at anatomy.org. Searchable transcript Captioned audiogram  Don't forget—HAPS members get a deep discount on AAA membership!   Even  More Web Meeting Ideas 12 minutes Yep, more Zoom. In this segment, Kevin talks about unintended harmful effects of banter, comments on home webcam locations, and turning off video. Plus some advice on backgrounds, both real and virtual. And stuff. Communication, Clarity, & Medical Errors | Episode 55 Pandemic Teaching Zooming While Black | Videoconferencing from our private spaces opens a lens on cultural authenticity, professional image, workplace code-switching and white privilege. (article by Shonda Buchanan) my-ap.us/2S3doEQ Krisp artificial-inteligence noise-eliminator theAPprofessor.org/krisp 4 Tips for Choosing the Best Virtual Backgrounds on Zoom Meetings (blog post) my-ap.us/2EBNN2Z pxhere (free photo site) pxhere.com Unsplash (free photo site) unsplash.com Some sample images suitable for Zoom virtual backgrounds: Dramatic sky my-ap.us/3j6Mk3F Wilderness my-ap.us/2G9yU8z Sunrise my-ap.us/2GcetHU Forest road my-ap.us/2S49P1f Misty my-ap.us/2FXAQ4j Broken sunlight my-ap.us/349gA7w Chalk board (black) my-ap.us/3cA78xW Green chalk boards my-ap.us/338dFwV Geometric shadows my-ap.us/3mWAL1f Wood planks my-ap.us/36dHiPb Book shelves my-ap.us/337maIg Leeds Library my-ap.us/335wwZl Gladstone's Libary my-ap.us/3cz4l86   Sponsored by HAPI Online Graduate Program 1 minute The Master of Science in Human Anatomy & Physiology Instruction—the MS-HAPI—is a graduate program for A&P teachers, especially for those who already have a graduate/professional degree. A combination of science courses (enough to qualify you to teach at the college level) and courses in contemporary instructional practice, this program helps you be your best in both on-campus and remote teaching. Kevin Patton is a faculty member in this program. Check it out! nycc.edu/hapi   Desirable Difficulty 15.5 minutes Robert Bjork proposed that the difficulties posed by retrieval practice, spacing, and interleaving are desirable difficulties that improve learning. But there are undesirable difficulties that do not help learning. Why must learning be difficult? How can we avoid undesirable difficulty? Hey, wait! Aren't we supposed to make learning easy for students?! Communication, Clarity, & Medical Errors | Episode 55 More on Spelling, Case, & Grammar | Episode 56 Desirable Difficulties Perspective on Learning (Robert Bjork's brief summary of his concept) my-ap.us/3kM0asE Making Things Hard on Yourself, But in a Good Way: Creating Desirable Difficulties to Enhance Learning (Elizabeth and Robert Bjork's contribution to Psychology in the Real World) my-ap.us/3i1Sv7J   Sponsored by HAPS 1 minute The Human Anatomy & Physiology Society (HAPS) is a sponsor of this podcast.  You can help appreciate their support by clicking the link below and checking out the many resources and benefits found there. Watch for virtual town hall meetings and upcoming regional meetings! Anatomy & Physiology Society theAPprofessor.org/haps   Our New Community 3.5 minutes Take The A&P Professor experience to a new level by joining the new online private community away from distracting social media platforms, tangle email threads, and the roiling sea of available webinars. 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Serendipity: Miracles Every Moment   drerin.tv soulciete.com IG & FB: @drerin.tv | @soulciete   The game of life is a game of boomerangs. Our thoughts, deeds and words return to us sooner or later with astounding accuracy. ~ Florence Scovel Shinn   On June 22, 1998, the Universe rotated 180 degrees. Not in reality, but in a multiverse kind of way. I was watching the classic movie The Wizard of Oz late at night, when I had a major epiphany.     This famous movie is about a young girl from Kansas named Dorothy who is swept away in her house by a cyclone with her dog Toto. Her home is dropped on top of the Wicked Witch of the East in the Land of Oz, where she is met by the Good Witch of the North. The witch gives her a pair of red ruby slippers and tells her that the only way for her to return home is for her to visit the Emerald City and ask the Wizard of Oz for help.     Following the yellow brick road, she meets a Scarecrow who wants a brain, a Tin Man who wants a heart, and a Cowardly Lion who wants courage, and invites them to go with her to meet the Wizard of Oz with the hope he can help all of them. After a long stream of events and realizing no one can help them, Glinda, the Good Witch, tells Dorothy, “You’ve always had the power to go back to Kansas.” Then the Scarecrow asked, “Then why didn’t you tell her before?” Glinda replies, “Because she wouldn’t have believed me. She had to learn it for herself.”     Right in front of my eyes, directly from the television screen, came the secret of Awakening, summed up in the greatest statement I had ever heard, “You had the power the entire time!”     Chills trickled down my arms. It was the answer I had been seeking; the key to my Awakening summed up in one clear statement backed by one thing that unlocked that power: Belief. The only thing that holds us back from Awakening to the power within is our beliefs!     ALL OF LIFE IS DESIGNED FOR YOUR AWAKENING     As you go down your own yellow brick road of life, experiencing the good times and the challenging times, realize that every drop of life is designed for your fate: Awakening. And, in your Awakening, all of life becomes a wonderland of your creation.     Wouldn’t it be amazing if you could walk through life having everything you have ever desired show up in a divinely succinct way? What if everywhere you went, miracles and manifestations of your choosing just appeared? What if life was one big serendipitous symphony, reflecting to you your innermost thoughts and desires? I invite you to consider that you are already there. Yes, everything you are experiencing in this moment is mirroring what is happening within your Consciousness in a serendipitous manner.     MIRACLES ARE ONLY GLITCHES IN YOUR CURRENT BELIEF SYSTEM     The word serendipity was first coined in 1754 by Horace Walpole to describe a fortunate happenstance, a pleasant surprise, wherein one discovers something seemingly without instigating a search to find it. Today, the Oxford English Dictionary (1989) defines serendipity as “the occurrence and development of events by chance in a happy or beneficial way.” Serendipity has always been a word synonymous with a positive, joyous response to our surroundings or life experience; a beautiful moment where life feels extraordinary. I am sure you can recall a time when you had the urge to phone a friend just seconds before they call you. Perhaps you have always wanted to live in San Francisco, and then your boss promotes you into your company’s San Francisco location. Maybe you meet someone and connect in a way that feels as if you have known that person your entire life. Perhaps you stumble upon a heart-shaped shell as you walk along the beach after asking the Universe for a sign to validate your feelings. Serendipitous instances are all around you. We all have experienced them in one way or another. It is the Universe giving you a reassuring wink that you are on the right path.     You see, serendipitous moments are not accidents. What if you could harness the power to tap into miraculous discoveries on a regular basis and attract them into your daily life? Wouldn’t that be amazing? Over the years, scientists have expressed incredulous awareness that major breakthroughs, discoveries, and inventions have occurred because of serendipitous moments. Had the apple fallen behind Sir Isaac Newton, rather than hitting him on his head, would he still have discovered gravity? Or would we still be mystified, contemplating whether what goes up really comes down? What if biologist, Alexander Fleming, had tidied his laboratory before taking a vacation? Would his staphylococci culture have had the time and atmosphere to grow a fungus that would lead him to discover penicillin? Would humanity have missed out on one of the most important discoveries in medicine? The invention of penicillin, a drug that has saved millions and millions of lives, is a direct result of this so-called accident (“ACS, n.d.).     Is a serendipitous instance what leads to innovation, or is it the observation of the moment and its psychological process which produces such phenomenon? Psychologist Drew Boyd (2013) poses that what we generally consider serendipitous inventions, can in fact, all be reduced to either a heuristic or an algorithm. If we chart serendipitous moments over time, patterns surface which can be used and codified as innovation on demand. Psychologically, the happy, joyous, rewarding feelings these discoveries bring can be misidentified as serendipitous, when in fact they are not coincidental. Serendipity is a name we have given the phenomenon of Consciousness.      If you take the Dalai Lama’s (2005) perception of the Universe as being one giant living organism like a structure of cells that all exist and operate in relation to one another, then surely you must acknowledge the potential of serendipitous patterns and how they relate to the vibrations you are putting out to the Universe. All your cells have their own Consciousness and can communicate with each other even when there is distance between them (Backster, 1968). Information and biological instruction are encoded in every living cell. Organisms grow and advance as these cells divide, reorganize, and replicate, carrying the encoded information and copying it into the new cell. A child can grow and develop because cells are able to replicate themselves, carrying all the encoding information and copying it into the new cell. Deoxyribonucleic acid (DNA) is the molecule that holds these codes, the genetic instructions that tell your cells how to develop and function (The Tech, 2013).     What if this encoded information was present not just in human beings and animals, but in every cell, atom, and particle that makes up the Universe? Information is transferred, duplicated, and functions and develops in every morsel of the Universe. All you need to do to communicate is listen and respond. Now imagine that these serendipitous moments are one way that the Universe is communicating with you.     In 2011, NASA confirmed they had located the nucleobases (molecules that carry information), Adenine and Guanine, in pieces of meteorites found in Alaska. The matter, proven to have come from space, contained these two essential building blocks for our very own DNA, telling us that meteorites and rocks impacting our planet were likely to have influenced the creation of life. Moreover, this information supports the notion that all matter is constructed from the same base (Choi, 2011).     Hypoxanthine and xanthine, two chemicals found in muscle tissue and used in biological processes, were also present in these rocks. The cells can communicate and instruct each other to grow when they are in close proximity, as proven by the development of the human body. The Universe has also been shown to be constructed from the same chemicals, which tells us that it no longer seems farfetched for us to be able to communicate with the Universe (Choi, 2011).     While many consider serendipity to be a magical force outside of our control, it is likely that these same people believe negative events or not-so-lucky occurrences do not follow the same patterns. Zemblanity, though not yet formally defined in dictionaries, is the term used to describe when unhappy, unlucky, negative, and expected discoveries are made by design (‘Zemblanity, n.d.). This seems easy to accept considering the human psychological need to use patterns to explain experience.   The Law of Correlation teaches you that the Universe reflects to you exactly what you emanate, as a direct result of your beliefs, thoughts, feelings, and actions. Positive, joyous, and serendipitous instances are not the only moments that give you guidance. Those troublesome situations, those negative instances, are also in place to move you forward. Having awareness of which thoughts and beliefs are keeping you from your full potential, and feeling and understanding when you are in tune with your inner being, will ultimately lead to the all-knowing connection: Awakening.     Serendipity and zemblanity are both wake up calls. The Universe is constantly nudging you to your Awakening and to be the greatest possible version of yourself. Your Subconscious is working behind the scenes 24/7, in cahoots with life, scheming up Divine plans of perfect reflections of your beliefs. The situations you experience are there to remind you that you are a miraculous being, and to present lessons to assist in your personal development.     The question is, are you ready to tap into your own unique personal power? Are you ready to experience love and serendipity everywhere in your life? Harnessing this ability is simple. Take anything that has happened in your life that you perceive as negative, find a way to see these circumstances as a blessing for you to wake up, and watch your life transform. For example, you might think of a time you lost your job. At the time, you may have considered yourself to be in a very unfortunate situation, but looking back, you can see how this situation has now placed you in a circumstance that is much better than what you ever expected.     It can be difficult to see the positive or galvanizing qualities that painful or difficult experiences provide, but if you focus, you will see how every single experience shaped, molded, and directed your life today. Every drop was created from the very core of your Consciousness. Some of you will wonder, “how can losing my job, or suffering a broken relationship or illness be the best thing that ever happened to me? How did I create it?” This is the shift that must take place for your Awakening. Once you open your heart with gratitude and acknowledge the painful circumstance as the impetus for your Awakening, then you will find a new opportunity to expand. When you truly believe that the Universe is working for your greater good, you will begin to observe how it is all miraculous, guiding you to the grandest version of yourself. You are the creator of your experience. The less you resist what you naturally want to attract, and the more you embrace the circumstances of your life as the opportunity to expand your Consciousness, the faster you will experience your Awakening.   Day 1 Practice   DECIDE TO WAKE UP     LAW OF PURE POTENTIAL At the core of you is infinite Consciousness; the connection between your individual Consciousness and your Cosmic Consciousness. There is an unending Divine Source within. All energy is pure potential until it is observed and informed. Your beliefs set the limit of demonstration of principle, which of itself is without limit. It is ready to fill everything because it is infinite. Your ability to have abundance is entirely a question of your own reciprocity to this Universal Intelligence.     Today’s practice is to DECIDE TO WAKE UP!   On a scale from 1-10 answer the following: (1= No, not at all & 10 = Yes, completely)   How committed are you to waking up? 1    2    3    4    5    6    7    8    9    10   How much do you believe you have the power to create your reality? 1    2    3    4    5    6    7    8    9    10   How much do you believe that you create your financial situation? 1    2    3    4    5    6    7    8    9    10   How much do you believe that you create the relationships in your life? 1    2    3    4    5    6    7    8    9    10   How much do you believe that you create the status of your health? 1    2    3    4    5    6    7    8    9    10   List 3 beautiful things you have created in your life: 1. ___________________________________________ 2. ___________________________________________ 3. ___________________________________________     I, _________________________ (your name) have decided to WAKE UP to the Truth of who I am and powerfully create a life I love!

-Deoxyribonucleic Acid -Progeny means descendent -DNA carries the living code for living organisms -Meteorites carry DNA through space -multi-cell organisms carry DNA in the nucleus -Adenine pairs with thymine -Guanine pairs with cytosine -these combos pair with sugar phosphate 'backbone' -fiber of every living being is negatively charged -oxidative phosphorylation breaks down food into energy for cells -every cell contains all information to rebuild entire body -cells have "limiters" which decide how each cell will behave -cancer is uncontrolled cell growth -RNA is unstable, cannot pack 2 meters(6feet) of information within each cell -2 to the 23rd power is the probability that you came into existence -Outside stress will rewrite your DNA -https://www.facebook.com/leigh.douglas.773?fref=ts -Lucas Ohio Pattie has a concert coming up Saturday, June 20, 2015 at Armando's 707 Marina Vista, Martinez, CA 94553 -Check him out at lucasohio.com -Keep an eye, or ear, out for his upcoming album

-Deoxyribonucleic Acid -Progeny means descendent -DNA carries the living code for living organisms -Meteorites carry DNA through space -multi-cell organisms carry DNA in the nucleus -Adenine pairs with thymine -Guanine pairs with cytosine -these combos pair with sugar phosphate 'backbone' -fiber of every living being is negatively charged -oxidative phosphorylation breaks down food into energy for cells -every cell contains all information to rebuild entire body -cells have "limiters" which decide how each cell will behave -cancer is uncontrolled cell growth -RNA is unstable, cannot pack 2 meters(6feet) of information within each cell -2 to the 23rd power is the probability that you came into existence -Outside stress will rewrite your DNA -https://www.facebook.com/leigh.douglas.773?fref=ts -Lucas Ohio Pattie has a concert coming up Saturday, June 20, 2015 at Armando's 707 Marina Vista, Martinez, CA 94553 -Check him out at lucasohio.com -Keep an eye, or ear, out for his upcoming album

Pioneers in Engineering is a UC Berkeley student-run project that provides STEM outreach in local high schools. PIE sponsors and supports a Spring semester robot competition. Guests include Vivek Nedyavila, Andrew Vanderburg, and David Huang. pioneers.berkeley.eduTranscriptsSpeaker 1: Spectrum's next Speaker 2: [inaudible].Speaker 1: [00:00:30] Welcome to spectrum the science and technology show on k a l x Berkeley, a biweekly 30 minute program bringing you interviews featuring bay area scientists and technologists as well as a calendar of local events and news. Speaker 3: Hi and good afternoon. My name is Brad swift and I'm the host of today's show. Our interview is with representatives of Pioneers and engineering, also known as Pi, [00:01:00] a UC Berkeley student run project. Since 2008 Pi has been doing stem outreach in bay area high schools, Pi sponsors and supports and annual spring semester robot competition, high school teams design, build and operate robots over seven weeks culminating in a thrilling final competition at the Lawrence Hall of Science Pineys UC Berkeley students to be mentors during this year as robot competition. Each [00:01:30] team gets a set of mentors to encourage and guide the team, helping them to realize their potential, explaining Pi, the stem outreach they do and why you may want to join our Vivek Nay Diallo Vala, Andrew Vanderburg and David Hawaiian onto the interview. I want to welcome you all to spectrum. And would you introduce yourselves and tell us what your major is? Speaker 1: Hi, my name is Vivek. I'm a UX major, electrical engineering and [00:02:00] computer sciences. I'm a junior. Speaker 4: I'm Andrew. I'm a senior physics and astronomy major. Speaker 3: Hi, my name is David. I'm a fourth year apply math and computer science major. Andrew, can you explain the history and goals of Pioneers and engineering? Speaker 4: Sure, so pioneer's engineering was founded in 2008 by Berkeley engineers. The general idea is that while there are a lot of good robotics competitions that provide science outreach to high school students, [00:02:30] a lot of them aren't very good at providing outreach to the students who need it. Most. The ones in the underprivileged schools. So pioneers in engineering or pie as we like to call it, is focusing on trying to provide that outreach. So we try to make it more sustainable so that they don't have to pay as much money every year and they don't have to have corporate sponsors. And we also try to make it more friendly so that they don't have to go out and search for their own mentors. They get their own mentors from UC Berkeley and we provide [inaudible]. Speaker 1: [00:03:00] And how did you decide on robots as the focus of your engineering challenge? Speaker 4: I think that robots are kind of a gimmick. They're cool, they're exciting and they have a lot of pop culture and references. But the lessons that we teach them could be applied to engineering, all sorts of different things. Perhaps we could do a science competition and get the same teaching out of it. Robots just provide something exciting. They provide a hook and they provide a climactic final competition where they can [00:03:30] have their robots, you know, compete head to head. [inaudible] Speaker 1: there is a certain kit aspect to what you're doing with the robots in terms of a known entity. A constraint. Speaker 4: Yeah. So we um, give them a very well-defined kit of parts which they can use so they don't have to start from scratch because building a robot from basic electronic components and pieces of metal or plywood is really hard. So we give them a good start. We give [00:04:00] them a kit which they can build upon. They don't have to do all of the electronics. They don't have to do a lot of the tedious work, but they can do something really cool with them in the end. Speaker 1: What's the funding source that you use for this competition? Speaker 4: We see corporate sponsorships. We go to companies like Google, Qualcomm, Boeing, and we ask them if they can support us, if they can. We advertise for them. We put their logos on our banners and our tee shirts [00:04:30] and they also get deductions for supporting charitable causes. [inaudible] Speaker 1: and are you a club? What is your organizational status? Speaker 4: We are technically a project of Tau Beta Pi, which is the engineering honor society and our finances and our organization go through them. Many of our members have or no, not affiliated with Beta Pi. They are recruited by us Speaker 1: beside the robot competition. Are there other projects within Pi [00:05:00] that you're working on? We have a team that actually goes to a high school called Ralph Bunche High School in West Oakland and this team does a program called Pie prep for these kids in which they have 13 or 14 modules of stem outreach kind of and they basically teach them cool things about science and technology and a little bit about robotics and physics and stuff like that and it's, it's once a week. It's intended to be fun and just spark their interest and also give them [00:05:30] a little bit of theoretical knowledge. This has been going very well this semester and from the results in the surveys that we've been taking, we're most likely gonna ramp it up next fall to even more schools. The exact number, we're not sure, but it's going to continue ramping up in the next few years and hopefully touch in the realm of 1314 schools in the area. We're hoping that this is going to be a very successful program and also inspire more interest in our robotics competition for the so we can have something good going on in the fall. It's [00:06:00] something in interest spring so it's like a year round kind of thing. Speaker 3: This is spectrum on k a l x Berkeley. Today's topic is pioneers in engineering. Three representatives from Pi join us. They are Vivek, Andrew and David. Andrew. How is it that high school's become involved in the [00:06:30] competition? Speaker 4: We do a lot of recruiting into high schools who fit our core mission, the ones who probably wouldn't be able to compete sustainably and the other robotics competitions that are out there. So we contact teachers and the sciences and we ask them if they're interested and if their students are interested in putting together a team and then they apply for a team and if we have room we'll take them. Speaker 3: What is the limit on teams? You have a capacity issue. Speaker 4: Yeah. We have a limit of about 20 teams could be up as many as 24 this year and the limitations [00:07:00] are put in place by our ability to produce kits and to provide mentors for them. We would rather have a good competition with 25 teams than one that stretched too thin with 35 Speaker 3: and do schools stick with it. Speaker 4: There is a core group of schools who seem to be building up somewhat of a legacy. They'll come back year after year. We actually just had our first student who is a four year high school participant in Pi Join Pi as a staff member [00:07:30] in college. Speaker 3: Great. That's the goal, right? In a way that's sort of the ideal. Andrew, when the teams are picked, they're picked by the teachers at the high schools. Speaker 4: The teams are I guess collected by the teachers at the high school, but they're based on interest. We've in the past tried to limit the number of people on the team, but we're moving away from that because um, we have a lot more mentors than we have in the past. Speaker 3: How do you try to keep the parody of the experience within [00:08:00] the teams and the resources that they have access to the equipment, the time spent? How do you, how do you try to balance all that? Keep everybody kind of on the same level. Speaker 4: So there are teams who have access to a machine shop in their high school and we can't provide that to everyone. But we do provide as a basic set of tools to anyone who wants them. We loan them out if they want to go to the high school and work with their team. And sometimes the high schools come to UC Berkeley and they can use our tools and our workspace in O'Brian Hall [00:08:30] in north side, we also try to ration the experience level of the mentors. We tried to provide the more experienced mentors to the less experienced teams. As a general rule, we try to provide equal experience and different types of engineering to each school. So each school should hope to have a mechanical engineer or someone who's mechanically inclined and someone who is electrically inclined or programming inclined. Speaker 1: And the number of mentors per team. Last year it ranged between four to six [00:09:00] of AVEC. Talk about your experience as a mentor on the robot competition. My experience at Ralph Bunche high school mentoring and was a series of ups and downs. But in the end it kind of culminated in something special. So started off with a few weeks of mentorship prep by um, Andrew and his mentorship team. They prepped us for what we would encounter a little bit of the social aspect of the kids, but mostly about the uh, technical mentorship. Ralph [00:09:30] onto high is a rather underprivileged high school in West Oakland. There were only three of them in the team and we had to struggle with people dropping out, people coming in because of the small size of the team, small quarrels that were involved, a lot of social issues that we were not as equipped for as mentors coming from UC Berkeley. Speaker 1: Um, not to mention the social barrier itself of where we have all come from in our lives compared to where these kids have come from. And [00:10:00] it was a really interesting experience for me because I actually have had a little bit of experience with kids from underprivileged backgrounds and the experience that I had in pulling my mentorship team into it with me trying to get everyone on the same page with these kids to not get frustrated with them, to not unequivocally say something and like have it mar the rest of our mentorship semesters. So it was a journey and it ended up being very rewarding, um, in the sense that [00:10:30] we got second place in the robotics competition and this team of three kids who were definitely the underdogs and it was just, you know, one of those quintessential underdog stories. They ended up getting second place and I was super proud of them. Speaker 1: So very rewarding experience. David, tell us about your experience last year as a mentor. I think the biggest and rather pleasant surprise, uh, during the tournament was at discrimination the week before and during the actual [00:11:00] tournament at the end of the season. The atmosphere was just absolutely incredible. We had, um, PAC has of spectators. We had epic music classing in the background and in both hers mining hardware. We had the scrimmage and the Lawrence Hom signs where we had to file tournament. The stage was very well prepared and when each team sent up their team members send their robot on the stage to compete. It gives you the feeling that you're these [00:11:30] stars on stage, sort of like maybe no gladiators in ancient Roman stadiums where you're the center of the attention of everyone around you and really at some level I feel like that's where colleges should be about is motivating students, motivating students, intellectual growth and also highlighting their achievements and I think in that sense Speaker 5: the Pi robotic competition has totally exceeded my expectation. I remember seeing a couple up the high school students [00:12:00] who ended up winning the competition, just crying on the stage and joy. I have no doubt that it had been a parade and really life changing experience for them. Speaker 3: Spectrum is on KALX Berkeley alternating Fridays. Today, we are talking with Vivec, Andrew and David about pioneers in engineering Speaker 1: as your involvement [00:12:30] in Pi giving you some insights into where you might want to go with your major. Speaker 4: My involvement in Pi has really been my first major experience in teaching and it turns out that teaching is a lot harder than you would think, especially teaching some of the difficult concepts that we have to do so quickly in our decal. It turns out that trying to break down the concepts into logical chunks and presenting them in a logical way is almost as hard, if not harder than learning them yourself. [00:13:00] So I found that teaching and learning to teach was a really good experience for me and it will help me presumably as I graduate and go to Grad school [inaudible] Speaker 1: because are you thinking of being a teacher? Speaker 4: I'm thinking of being hopefully a professor in the future. I hope that my experience in Pi will give me a leg up from working on that and hopefully make it easier for my students to learn in the future. Speaker 3: [inaudible] David, anything. Yeah. Speaker 5: So I try and Pi as a part of my effort to explore [00:13:30] more in computer science, which I started taking classes last year and I have to say during the course of last semesters tournament, I really enjoy working with the staff member, other fellow UC Berkeley students and Pi. And I also really enjoy working with the high school students on my team to the extent that, uh, I'm starting to look more and more into the idea of working at a technology startup. And I'm also fairly sure I'm going to do computer science as a second major along with math. [00:14:00] And so in that sense, I think it's really solidify my interests in this field. Speaker 1: VEC, how has pi affected your plans for the future? I've actually had, I guess in the last few weeks to think about this very seriously. And through talking with a number of people in Pie, I'm very, very inclined to do something kind of like this as a job in the future. Like being scientific outreach. Yeah, exactly. Scientific kind [00:14:30] of stem education. Stem outreach. Yeah. So there's um, a company called sparkfun that we have grown closer to over the last year and this is kind of exactly what they do. They have a sparkfun kit circuit skit and it's a solderless circuit skit where they can bring it to elementary, middle school classrooms and have these kids play around with circuits. They want to fund a trip across the nation teaching stuff like this to little kid. Just seeing things like this happen in the world makes me really rethink, do [00:15:00] I just want to become a fabrications engineer or something or like do I want to be a programmer or do I need something like this without there the risks are higher, but the reward, the potential reward is greater. Yeah, that's, that's how it's changed my outlook. What sort of a time commitment is there to being a Pi staffer or a mentor? Speaker 4: So being a mentor, we ask that you attend a two hour day call once a week. We ask that you mentor your teams [00:15:30] for at least two hours a week. And we also ask that you do a five minute progress report so that we know how your teams are doing. So if you add in transportation time, it's probably adds up to about six to eight hours a week of time commitment. That won't be distributed evenly necessarily because there'll be weeks where you have weekend events, which lasts all day. But I think that most peer mentors have found that the time commitment really isn't a problem because by the time that the time coming and gets large, [00:16:00] you really want to be there and it's a lot of fun. Speaker 1: And then for staff, so I know this isn't the time for staff to get involved or are you always looking for staff or is it really just at the fall? Speaker 4: So we're always looking for staff. We do need mentors more than staff at this moment, but as a staff member, the time commitment is probably larger, probably order of 10 hours a week for the seven or eight weeks around the competition. At other times it's less, more [00:16:30] of a year long job than this intense seven week period as it would be for a mentor. Speaker 1: Andrew, if you want to become a mentor, what's the process? Okay. Speaker 4: For people who are interested in being mentors to the high school students, we are going to have a mentoring decal which starts in early February. On February 4th that decal will run from six to 8:00 PM on Mondays and Thursdays. And it's once a week. You choose one of those two times and uh, you come to that, you learn [00:17:00] about robotics and then we scheduled for a seven week period starting in March time for you to go to your high schools every week. That's flexible, depends on your schedule, on the high school schedule. The final competition will wrap up around April 28th Speaker 1: and the kind of people you're looking for talk about who can be a mentor, Speaker 4: right? So we accept mentors from every background. We believe that our decal will teach them the basics that can get them [00:17:30] to help their high school students out. And we also believe that learning about engineering is not the only purpose of Pi. We think that other students from other backgrounds can contribute just as much as engineers can because in the end it's not just about teaching them to be engineers, it's about teaching them to go to college, what it's like to be in college, what it's like, enjoy learning and some of our best mentors in the past have not been engineers. Speaker 6: [inaudible]Speaker 3: [00:18:00] pioneers in engineering on spectrum detailing their stem outreach. This is k a l X. Speaker 6: [inaudible].Speaker 3: Do you all find Pi to be a real supportive community for your own personal interests as well as the collective interest of doing the competition and start with the Vac, right. [00:18:30] Then we'll go around. Speaker 1: For me it's the spirit of kind of like self-expression. You're doing something very special for these kids. It's a form of giving someone else what I had when I was a kid in the form of my dad or in the form of other people in my life who influenced me towards engineering and to motivate kids or like allow them to have that confidence in themselves. To go towards stem and at least higher education, one of the main goals of Pie. [00:19:00] Don't be afraid to apply to college and stuff like that. That form of self expression and just kind of helping these kids and self fulfillment through that, that the perk that I get, Speaker 4: I feel as if Pi is a really supportive community because even though the going is often tough as a staff member, there's a lot of pressure because he wants to deliver a good competition to the students. Everyone's willing to help each other out. And I think that it's a really good community to have around you because [00:19:30] even though we're all doing a lot of work and sometimes we can get stressed, we remember that we have each other and that we're all working towards a common goal, which is to give these students a good educational experience. And that's something that a lot of them don't get in school. Speaker 5: So coming from the perspective of surf a semi insider outsider, uh, as a pass mentor, um, I think Pi has given me the opportunity to meet a lot of other people who are similarly interested in science and engineering [00:20:00] from the perspective that these are wonderful things to learn about and to see happen in everyday life instead of just something that you learned together job. And going along that perspective, having met all these really interesting people, empire has given me more social avenues to while to hang out, for instance, for Thanksgiving or just took walk around campus and to know that there are all these people around me who are also likewise striving for a similar goal. And that's comforting to know. Speaker 3: [00:20:30] Vivek, Andrew and David, thanks very much for being on spectrum. Thank you. Thank you for having us. Speaker 2: [inaudible] now our calendar of local science and technology events over the next two weeks, Renee Rao and Ricardo [inaudible] present the calendar. Speaker 7: [00:21:00] Okay. Dr. Shannon Bennett, associate curator of microbiology at the California Academy of Sciences. We'll be hosting a lecture by HIV expert, Dr Leo Weinberger, who will discuss the engineering of a retro virus to cure HIV. While progress has been made in controlling the virus with heavy cocktails or combinations of drugs, more virulent and resistant varieties continue to arise, Weinberger will explore his idea of using the same virus that causes the disease to deliver [00:21:30] the cure. The event will be held at 12:00 PM on Saturday, January 26 tickets will be on sale at the California Academy of Sciences website, $15 for adults and seven for students or seniors. Martin Hellman, Speaker 8: the co-inventor of public key cryptography is presenting the free Stanford engineering hero lecture at the Long Engineering Center at Stanford on Tuesday, January 29th from seven to 9:00 PM [00:22:00] with reception after his talk on the wisdom of foolishness, explorers, how tilting at windmills can turn out. Well in the 1970s Homan was competing with the national security agency who had a much larger budgets than he had, and it was warned that the NSA may classify any accomplishments he made. Despite this with help from Whitfield Diffie and Ralph Merkle, Hellman spearheaded systems that are still used to secure Chileans of dollars of financial [00:22:30] transactions a day. Visit www. That's certain.com for more info Speaker 7: east based first nerd night of 2013 we'll feature three Speakers, Daniel Cohen, a phd candidate in the joint UC Berkeley UCLA program. We'll speak about the theme of collective behavior, discussing the mechanism for everything from hurting sheep to sell your cooperation. Andrew Pike, a u Penn geologist by trade has also been [00:23:00] a contender in the competitive rock paper, Scissors League of Philadelphia. He will discuss some of the surprisingly complex strategies to the game. Lena Nielsen, the Innovation Director at the Bluhm center for developing economies at UC Berkeley. We'll explore technological solutions to extreme global problems that are also financially feasible. The event will start at eight but doors open at seven the event is held on January 28th at the new parkway located at four seven four [00:23:30] 24th street in Oakland. Science fans of all ages are welcome and can purchase the $8 tickets online. Speaker 8: On Tuesday, February 5th at 6:00 PM the Felix Block, a professor in theoretical physics at and the director of the Stanford Institute for theoretical physicist, Leonard Susskind is talking to the Commonwealth Club of San Francisco located at five nine five market street. The presentation is entitled the theoretical minimum, [00:24:00] what you need to know to start doing physics Susskind. We'll discuss how to learn more about physics and how to think more like a scientist. He will provide a toolkit to help people advance at their own pace. The cost is $20 to the public, $8 to members and $7 to students. Visit www that commonwealth club.org four tickets. Speaker 7: UC Berkeley's center for emerging and neglected diseases will hold its fifth annual [00:24:30] symposium this year. A variety of Speakers will present their work in various areas of infection and host response. The theme of the symposium, the keynote Speaker, dawn Ghanem will explore new developments in malaria drugs across the world. Sarah Sawyer, another Speaker. We'll discuss what typically keeps animal viruses from infecting humans. Other topics will include emerging African biomedical research on HIV AIDS, mycobacterium [00:25:00] tuberculosis, and new testing protocols for infectious diseases in developing countries. The symposium will be held in Stanley Hall on the UC Berkeley campus on February 11th from 9:00 AM to 5:00 PM it's open to anyone who registers@www.global health.berkeley.edu Speaker 2: [inaudible]Speaker 8: [00:25:30] the two news items [inaudible] that can Renee, university of Cambridge researchers published an article in Nature Chemistry on January 20th that indicates DNA conform not only the classic double stranded Helix, but also structures that are made from four strands. It's been thought that these square shaped g quadroplex structures may form in the DNA of cells, but this paper is one of the first to provide evidence that they do exist [00:26:00] in human cells. They forum when four Guanines make a special type of hydrogen bond. Speaker 8: The telomeres that protect Chromosomal DNA are Irish and Guanine and research points to quadroplex formation. And there is evidence that suggests quadruplex formation could damage these Tila mirrors and may play a role in how certain genes contribute to cancer. The team created a simple antibody that stabilizes these g quadroplex structures and showed how the structures are [00:26:30] formed and trapped in human DNA. When describing the long term goals of the research, the team told science daily that many current cancer treatments attack DNA, but it's not clear what the rules are. We don't aware in the genome some of them react. It can be a scattergun approach. The possibility that particular cancer cells harboring genes with these motifs can now be targets and appear to be more vulnerable to interference than normal cells is that thrilling prospect. Speaker 7: Okay. A joint [00:27:00] UC Berkeley Duke University Study of couches across the nation reveals a disturbingly high percentage of our sofas contained noticeable levels of toxins. 102 couches in 27 states were examined in this study. Of these 41% were found to contain the chemical chlorinated Tris, a known carcinogen. 17% of the couches also contain Penta BDE, which can cause hormonal disruptions. While chlorinated Tris was banned [00:27:30] from use in children's clothing in the 1970s it continues to be routinely used by companies seeking to make foam furniture more fire resistant. Currently, California State Law requires a certain degree of flame retardancy, but does not require that the types or amount of chemicals used to achieve this be disclosed. Well, most cotton will or down catches are naturally flame resistant. Any foam catches will almost certainly require added chemicals to meet current standards. Last June, [00:28:00] Governor Jerry Brown advised the state legislature to reform flammability standards for furniture. Once the new regulations are adopted, the chemical free couches should be available. Speaker 2: [inaudible] [inaudible] [inaudible]. The music art during the show is by on a David from his album folk and acoustic released under [00:28:30] a creative Commons license 3.0 attributes. [inaudible] [inaudible] [inaudible] [inaudible]. [00:29:00] Yeah. Thank you for listening to spectrum. If you have common staff to show, please send them to us via email. All right, email address is spectrum dot klx@yahoo.com join us in two weeks. This same time. Speaker 9: [inaudible] [00:29:30] [inaudible] [inaudible]. Hosted on Acast. See acast.com/privacy for more information.

Pioneers in Engineering is a UC Berkeley student-run project that provides STEM outreach in local high schools. PIE sponsors and supports a Spring semester robot competition. Guests include Vivek Nedyavila, Andrew Vanderburg, and David Huang. pioneers.berkeley.eduTranscriptsSpeaker 1: Spectrum's next Speaker 2: [inaudible].Speaker 1: [00:00:30] Welcome to spectrum the science and technology show on k a l x Berkeley, a biweekly 30 minute program bringing you interviews featuring bay area scientists and technologists as well as a calendar of local events and news. Speaker 3: Hi and good afternoon. My name is Brad swift and I'm the host of today's show. Our interview is with representatives of Pioneers and engineering, also known as Pi, [00:01:00] a UC Berkeley student run project. Since 2008 Pi has been doing stem outreach in bay area high schools, Pi sponsors and supports and annual spring semester robot competition, high school teams design, build and operate robots over seven weeks culminating in a thrilling final competition at the Lawrence Hall of Science Pineys UC Berkeley students to be mentors during this year as robot competition. Each [00:01:30] team gets a set of mentors to encourage and guide the team, helping them to realize their potential, explaining Pi, the stem outreach they do and why you may want to join our Vivek Nay Diallo Vala, Andrew Vanderburg and David Hawaiian onto the interview. I want to welcome you all to spectrum. And would you introduce yourselves and tell us what your major is? Speaker 1: Hi, my name is Vivek. I'm a UX major, electrical engineering and [00:02:00] computer sciences. I'm a junior. Speaker 4: I'm Andrew. I'm a senior physics and astronomy major. Speaker 3: Hi, my name is David. I'm a fourth year apply math and computer science major. Andrew, can you explain the history and goals of Pioneers and engineering? Speaker 4: Sure, so pioneer's engineering was founded in 2008 by Berkeley engineers. The general idea is that while there are a lot of good robotics competitions that provide science outreach to high school students, [00:02:30] a lot of them aren't very good at providing outreach to the students who need it. Most. The ones in the underprivileged schools. So pioneers in engineering or pie as we like to call it, is focusing on trying to provide that outreach. So we try to make it more sustainable so that they don't have to pay as much money every year and they don't have to have corporate sponsors. And we also try to make it more friendly so that they don't have to go out and search for their own mentors. They get their own mentors from UC Berkeley and we provide [inaudible]. Speaker 1: [00:03:00] And how did you decide on robots as the focus of your engineering challenge? Speaker 4: I think that robots are kind of a gimmick. They're cool, they're exciting and they have a lot of pop culture and references. But the lessons that we teach them could be applied to engineering, all sorts of different things. Perhaps we could do a science competition and get the same teaching out of it. Robots just provide something exciting. They provide a hook and they provide a climactic final competition where they can [00:03:30] have their robots, you know, compete head to head. [inaudible] Speaker 1: there is a certain kit aspect to what you're doing with the robots in terms of a known entity. A constraint. Speaker 4: Yeah. So we um, give them a very well-defined kit of parts which they can use so they don't have to start from scratch because building a robot from basic electronic components and pieces of metal or plywood is really hard. So we give them a good start. We give [00:04:00] them a kit which they can build upon. They don't have to do all of the electronics. They don't have to do a lot of the tedious work, but they can do something really cool with them in the end. Speaker 1: What's the funding source that you use for this competition? Speaker 4: We see corporate sponsorships. We go to companies like Google, Qualcomm, Boeing, and we ask them if they can support us, if they can. We advertise for them. We put their logos on our banners and our tee shirts [00:04:30] and they also get deductions for supporting charitable causes. [inaudible] Speaker 1: and are you a club? What is your organizational status? Speaker 4: We are technically a project of Tau Beta Pi, which is the engineering honor society and our finances and our organization go through them. Many of our members have or no, not affiliated with Beta Pi. They are recruited by us Speaker 1: beside the robot competition. Are there other projects within Pi [00:05:00] that you're working on? We have a team that actually goes to a high school called Ralph Bunche High School in West Oakland and this team does a program called Pie prep for these kids in which they have 13 or 14 modules of stem outreach kind of and they basically teach them cool things about science and technology and a little bit about robotics and physics and stuff like that and it's, it's once a week. It's intended to be fun and just spark their interest and also give them [00:05:30] a little bit of theoretical knowledge. This has been going very well this semester and from the results in the surveys that we've been taking, we're most likely gonna ramp it up next fall to even more schools. The exact number, we're not sure, but it's going to continue ramping up in the next few years and hopefully touch in the realm of 1314 schools in the area. We're hoping that this is going to be a very successful program and also inspire more interest in our robotics competition for the so we can have something good going on in the fall. It's [00:06:00] something in interest spring so it's like a year round kind of thing. Speaker 3: This is spectrum on k a l x Berkeley. Today's topic is pioneers in engineering. Three representatives from Pi join us. They are Vivek, Andrew and David. Andrew. How is it that high school's become involved in the [00:06:30] competition? Speaker 4: We do a lot of recruiting into high schools who fit our core mission, the ones who probably wouldn't be able to compete sustainably and the other robotics competitions that are out there. So we contact teachers and the sciences and we ask them if they're interested and if their students are interested in putting together a team and then they apply for a team and if we have room we'll take them. Speaker 3: What is the limit on teams? You have a capacity issue. Speaker 4: Yeah. We have a limit of about 20 teams could be up as many as 24 this year and the limitations [00:07:00] are put in place by our ability to produce kits and to provide mentors for them. We would rather have a good competition with 25 teams than one that stretched too thin with 35 Speaker 3: and do schools stick with it. Speaker 4: There is a core group of schools who seem to be building up somewhat of a legacy. They'll come back year after year. We actually just had our first student who is a four year high school participant in Pi Join Pi as a staff member [00:07:30] in college. Speaker 3: Great. That's the goal, right? In a way that's sort of the ideal. Andrew, when the teams are picked, they're picked by the teachers at the high schools. Speaker 4: The teams are I guess collected by the teachers at the high school, but they're based on interest. We've in the past tried to limit the number of people on the team, but we're moving away from that because um, we have a lot more mentors than we have in the past. Speaker 3: How do you try to keep the parody of the experience within [00:08:00] the teams and the resources that they have access to the equipment, the time spent? How do you, how do you try to balance all that? Keep everybody kind of on the same level. Speaker 4: So there are teams who have access to a machine shop in their high school and we can't provide that to everyone. But we do provide as a basic set of tools to anyone who wants them. We loan them out if they want to go to the high school and work with their team. And sometimes the high schools come to UC Berkeley and they can use our tools and our workspace in O'Brian Hall [00:08:30] in north side, we also try to ration the experience level of the mentors. We tried to provide the more experienced mentors to the less experienced teams. As a general rule, we try to provide equal experience and different types of engineering to each school. So each school should hope to have a mechanical engineer or someone who's mechanically inclined and someone who is electrically inclined or programming inclined. Speaker 1: And the number of mentors per team. Last year it ranged between four to six [00:09:00] of AVEC. Talk about your experience as a mentor on the robot competition. My experience at Ralph Bunche high school mentoring and was a series of ups and downs. But in the end it kind of culminated in something special. So started off with a few weeks of mentorship prep by um, Andrew and his mentorship team. They prepped us for what we would encounter a little bit of the social aspect of the kids, but mostly about the uh, technical mentorship. Ralph [00:09:30] onto high is a rather underprivileged high school in West Oakland. There were only three of them in the team and we had to struggle with people dropping out, people coming in because of the small size of the team, small quarrels that were involved, a lot of social issues that we were not as equipped for as mentors coming from UC Berkeley. Speaker 1: Um, not to mention the social barrier itself of where we have all come from in our lives compared to where these kids have come from. And [00:10:00] it was a really interesting experience for me because I actually have had a little bit of experience with kids from underprivileged backgrounds and the experience that I had in pulling my mentorship team into it with me trying to get everyone on the same page with these kids to not get frustrated with them, to not unequivocally say something and like have it mar the rest of our mentorship semesters. So it was a journey and it ended up being very rewarding, um, in the sense that [00:10:30] we got second place in the robotics competition and this team of three kids who were definitely the underdogs and it was just, you know, one of those quintessential underdog stories. They ended up getting second place and I was super proud of them. Speaker 1: So very rewarding experience. David, tell us about your experience last year as a mentor. I think the biggest and rather pleasant surprise, uh, during the tournament was at discrimination the week before and during the actual [00:11:00] tournament at the end of the season. The atmosphere was just absolutely incredible. We had, um, PAC has of spectators. We had epic music classing in the background and in both hers mining hardware. We had the scrimmage and the Lawrence Hom signs where we had to file tournament. The stage was very well prepared and when each team sent up their team members send their robot on the stage to compete. It gives you the feeling that you're these [00:11:30] stars on stage, sort of like maybe no gladiators in ancient Roman stadiums where you're the center of the attention of everyone around you and really at some level I feel like that's where colleges should be about is motivating students, motivating students, intellectual growth and also highlighting their achievements and I think in that sense Speaker 5: the Pi robotic competition has totally exceeded my expectation. I remember seeing a couple up the high school students [00:12:00] who ended up winning the competition, just crying on the stage and joy. I have no doubt that it had been a parade and really life changing experience for them. Speaker 3: Spectrum is on KALX Berkeley alternating Fridays. Today, we are talking with Vivec, Andrew and David about pioneers in engineering Speaker 1: as your involvement [00:12:30] in Pi giving you some insights into where you might want to go with your major. Speaker 4: My involvement in Pi has really been my first major experience in teaching and it turns out that teaching is a lot harder than you would think, especially teaching some of the difficult concepts that we have to do so quickly in our decal. It turns out that trying to break down the concepts into logical chunks and presenting them in a logical way is almost as hard, if not harder than learning them yourself. [00:13:00] So I found that teaching and learning to teach was a really good experience for me and it will help me presumably as I graduate and go to Grad school [inaudible] Speaker 1: because are you thinking of being a teacher? Speaker 4: I'm thinking of being hopefully a professor in the future. I hope that my experience in Pi will give me a leg up from working on that and hopefully make it easier for my students to learn in the future. Speaker 3: [inaudible] David, anything. Yeah. Speaker 5: So I try and Pi as a part of my effort to explore [00:13:30] more in computer science, which I started taking classes last year and I have to say during the course of last semesters tournament, I really enjoy working with the staff member, other fellow UC Berkeley students and Pi. And I also really enjoy working with the high school students on my team to the extent that, uh, I'm starting to look more and more into the idea of working at a technology startup. And I'm also fairly sure I'm going to do computer science as a second major along with math. [00:14:00] And so in that sense, I think it's really solidify my interests in this field. Speaker 1: VEC, how has pi affected your plans for the future? I've actually had, I guess in the last few weeks to think about this very seriously. And through talking with a number of people in Pie, I'm very, very inclined to do something kind of like this as a job in the future. Like being scientific outreach. Yeah, exactly. Scientific kind [00:14:30] of stem education. Stem outreach. Yeah. So there's um, a company called sparkfun that we have grown closer to over the last year and this is kind of exactly what they do. They have a sparkfun kit circuit skit and it's a solderless circuit skit where they can bring it to elementary, middle school classrooms and have these kids play around with circuits. They want to fund a trip across the nation teaching stuff like this to little kid. Just seeing things like this happen in the world makes me really rethink, do [00:15:00] I just want to become a fabrications engineer or something or like do I want to be a programmer or do I need something like this without there the risks are higher, but the reward, the potential reward is greater. Yeah, that's, that's how it's changed my outlook. What sort of a time commitment is there to being a Pi staffer or a mentor? Speaker 4: So being a mentor, we ask that you attend a two hour day call once a week. We ask that you mentor your teams [00:15:30] for at least two hours a week. And we also ask that you do a five minute progress report so that we know how your teams are doing. So if you add in transportation time, it's probably adds up to about six to eight hours a week of time commitment. That won't be distributed evenly necessarily because there'll be weeks where you have weekend events, which lasts all day. But I think that most peer mentors have found that the time commitment really isn't a problem because by the time that the time coming and gets large, [00:16:00] you really want to be there and it's a lot of fun. Speaker 1: And then for staff, so I know this isn't the time for staff to get involved or are you always looking for staff or is it really just at the fall? Speaker 4: So we're always looking for staff. We do need mentors more than staff at this moment, but as a staff member, the time commitment is probably larger, probably order of 10 hours a week for the seven or eight weeks around the competition. At other times it's less, more [00:16:30] of a year long job than this intense seven week period as it would be for a mentor. Speaker 1: Andrew, if you want to become a mentor, what's the process? Okay. Speaker 4: For people who are interested in being mentors to the high school students, we are going to have a mentoring decal which starts in early February. On February 4th that decal will run from six to 8:00 PM on Mondays and Thursdays. And it's once a week. You choose one of those two times and uh, you come to that, you learn [00:17:00] about robotics and then we scheduled for a seven week period starting in March time for you to go to your high schools every week. That's flexible, depends on your schedule, on the high school schedule. The final competition will wrap up around April 28th Speaker 1: and the kind of people you're looking for talk about who can be a mentor, Speaker 4: right? So we accept mentors from every background. We believe that our decal will teach them the basics that can get them [00:17:30] to help their high school students out. And we also believe that learning about engineering is not the only purpose of Pi. We think that other students from other backgrounds can contribute just as much as engineers can because in the end it's not just about teaching them to be engineers, it's about teaching them to go to college, what it's like to be in college, what it's like, enjoy learning and some of our best mentors in the past have not been engineers. Speaker 6: [inaudible]Speaker 3: [00:18:00] pioneers in engineering on spectrum detailing their stem outreach. This is k a l X. Speaker 6: [inaudible].Speaker 3: Do you all find Pi to be a real supportive community for your own personal interests as well as the collective interest of doing the competition and start with the Vac, right. [00:18:30] Then we'll go around. Speaker 1: For me it's the spirit of kind of like self-expression. You're doing something very special for these kids. It's a form of giving someone else what I had when I was a kid in the form of my dad or in the form of other people in my life who influenced me towards engineering and to motivate kids or like allow them to have that confidence in themselves. To go towards stem and at least higher education, one of the main goals of Pie. [00:19:00] Don't be afraid to apply to college and stuff like that. That form of self expression and just kind of helping these kids and self fulfillment through that, that the perk that I get, Speaker 4: I feel as if Pi is a really supportive community because even though the going is often tough as a staff member, there's a lot of pressure because he wants to deliver a good competition to the students. Everyone's willing to help each other out. And I think that it's a really good community to have around you because [00:19:30] even though we're all doing a lot of work and sometimes we can get stressed, we remember that we have each other and that we're all working towards a common goal, which is to give these students a good educational experience. And that's something that a lot of them don't get in school. Speaker 5: So coming from the perspective of surf a semi insider outsider, uh, as a pass mentor, um, I think Pi has given me the opportunity to meet a lot of other people who are similarly interested in science and engineering [00:20:00] from the perspective that these are wonderful things to learn about and to see happen in everyday life instead of just something that you learned together job. And going along that perspective, having met all these really interesting people, empire has given me more social avenues to while to hang out, for instance, for Thanksgiving or just took walk around campus and to know that there are all these people around me who are also likewise striving for a similar goal. And that's comforting to know. Speaker 3: [00:20:30] Vivek, Andrew and David, thanks very much for being on spectrum. Thank you. Thank you for having us. Speaker 2: [inaudible] now our calendar of local science and technology events over the next two weeks, Renee Rao and Ricardo [inaudible] present the calendar. Speaker 7: [00:21:00] Okay. Dr. Shannon Bennett, associate curator of microbiology at the California Academy of Sciences. We'll be hosting a lecture by HIV expert, Dr Leo Weinberger, who will discuss the engineering of a retro virus to cure HIV. While progress has been made in controlling the virus with heavy cocktails or combinations of drugs, more virulent and resistant varieties continue to arise, Weinberger will explore his idea of using the same virus that causes the disease to deliver [00:21:30] the cure. The event will be held at 12:00 PM on Saturday, January 26 tickets will be on sale at the California Academy of Sciences website, $15 for adults and seven for students or seniors. Martin Hellman, Speaker 8: the co-inventor of public key cryptography is presenting the free Stanford engineering hero lecture at the Long Engineering Center at Stanford on Tuesday, January 29th from seven to 9:00 PM [00:22:00] with reception after his talk on the wisdom of foolishness, explorers, how tilting at windmills can turn out. Well in the 1970s Homan was competing with the national security agency who had a much larger budgets than he had, and it was warned that the NSA may classify any accomplishments he made. Despite this with help from Whitfield Diffie and Ralph Merkle, Hellman spearheaded systems that are still used to secure Chileans of dollars of financial [00:22:30] transactions a day. Visit www. That's certain.com for more info Speaker 7: east based first nerd night of 2013 we'll feature three Speakers, Daniel Cohen, a phd candidate in the joint UC Berkeley UCLA program. We'll speak about the theme of collective behavior, discussing the mechanism for everything from hurting sheep to sell your cooperation. Andrew Pike, a u Penn geologist by trade has also been [00:23:00] a contender in the competitive rock paper, Scissors League of Philadelphia. He will discuss some of the surprisingly complex strategies to the game. Lena Nielsen, the Innovation Director at the Bluhm center for developing economies at UC Berkeley. We'll explore technological solutions to extreme global problems that are also financially feasible. The event will start at eight but doors open at seven the event is held on January 28th at the new parkway located at four seven four [00:23:30] 24th street in Oakland. Science fans of all ages are welcome and can purchase the $8 tickets online. Speaker 8: On Tuesday, February 5th at 6:00 PM the Felix Block, a professor in theoretical physics at and the director of the Stanford Institute for theoretical physicist, Leonard Susskind is talking to the Commonwealth Club of San Francisco located at five nine five market street. The presentation is entitled the theoretical minimum, [00:24:00] what you need to know to start doing physics Susskind. We'll discuss how to learn more about physics and how to think more like a scientist. He will provide a toolkit to help people advance at their own pace. The cost is $20 to the public, $8 to members and $7 to students. Visit www that commonwealth club.org four tickets. Speaker 7: UC Berkeley's center for emerging and neglected diseases will hold its fifth annual [00:24:30] symposium this year. A variety of Speakers will present their work in various areas of infection and host response. The theme of the symposium, the keynote Speaker, dawn Ghanem will explore new developments in malaria drugs across the world. Sarah Sawyer, another Speaker. We'll discuss what typically keeps animal viruses from infecting humans. Other topics will include emerging African biomedical research on HIV AIDS, mycobacterium [00:25:00] tuberculosis, and new testing protocols for infectious diseases in developing countries. The symposium will be held in Stanley Hall on the UC Berkeley campus on February 11th from 9:00 AM to 5:00 PM it's open to anyone who registers@www.global health.berkeley.edu Speaker 2: [inaudible]Speaker 8: [00:25:30] the two news items [inaudible] that can Renee, university of Cambridge researchers published an article in Nature Chemistry on January 20th that indicates DNA conform not only the classic double stranded Helix, but also structures that are made from four strands. It's been thought that these square shaped g quadroplex structures may form in the DNA of cells, but this paper is one of the first to provide evidence that they do exist [00:26:00] in human cells. They forum when four Guanines make a special type of hydrogen bond. Speaker 8: The telomeres that protect Chromosomal DNA are Irish and Guanine and research points to quadroplex formation. And there is evidence that suggests quadruplex formation could damage these Tila mirrors and may play a role in how certain genes contribute to cancer. The team created a simple antibody that stabilizes these g quadroplex structures and showed how the structures are [00:26:30] formed and trapped in human DNA. When describing the long term goals of the research, the team told science daily that many current cancer treatments attack DNA, but it's not clear what the rules are. We don't aware in the genome some of them react. It can be a scattergun approach. The possibility that particular cancer cells harboring genes with these motifs can now be targets and appear to be more vulnerable to interference than normal cells is that thrilling prospect. Speaker 7: Okay. A joint [00:27:00] UC Berkeley Duke University Study of couches across the nation reveals a disturbingly high percentage of our sofas contained noticeable levels of toxins. 102 couches in 27 states were examined in this study. Of these 41% were found to contain the chemical chlorinated Tris, a known carcinogen. 17% of the couches also contain Penta BDE, which can cause hormonal disruptions. While chlorinated Tris was banned [00:27:30] from use in children's clothing in the 1970s it continues to be routinely used by companies seeking to make foam furniture more fire resistant. Currently, California State Law requires a certain degree of flame retardancy, but does not require that the types or amount of chemicals used to achieve this be disclosed. Well, most cotton will or down catches are naturally flame resistant. Any foam catches will almost certainly require added chemicals to meet current standards. Last June, [00:28:00] Governor Jerry Brown advised the state legislature to reform flammability standards for furniture. Once the new regulations are adopted, the chemical free couches should be available. Speaker 2: [inaudible] [inaudible] [inaudible]. The music art during the show is by on a David from his album folk and acoustic released under [00:28:30] a creative Commons license 3.0 attributes. [inaudible] [inaudible] [inaudible] [inaudible]. [00:29:00] Yeah. Thank you for listening to spectrum. If you have common staff to show, please send them to us via email. All right, email address is spectrum dot klx@yahoo.com join us in two weeks. This same time. Speaker 9: [inaudible] [00:29:30] [inaudible] [inaudible]. See acast.com/privacy for privacy and opt-out information.

Ulrich Hübscher, Institute of Veterinary Biochemistry and Molecular Biology, University of Zürich-Irchel, Zürich, SWITZERLAND speaks on "Oxygen as a friend and enemy: how to combat the mutational potential of 8-oxo-guanine". This seminar has been recorded by ICGEB Trieste

Dictyostelium, an amoeboid motile cell, harbors several paralogous Sec7 genes that encode members of three distinct subfamilies of the Sec7 superfamily of Guanine nucleotide exchange factors. Among them are proteins of the GBF/BIG family present in all eukaryotes. The third subfamily represented with three members in D. discoideum is the cytohesin family that has been thought to be metazoan specific. Cytohesins are characterized by a Sec7 PH tandem domain and have roles in cell adhesion and migration. Dictyostelium SecG exhibits highest homologies to the cytohesins. It harbors at its amino terminus several ankyrin repeats that are followed by the Sec7 PH tandem domain. Mutants lacking SecG show reduced cell-substratum adhesion whereas cell-cell adhesion that is important for development is not affected. Accordingly, multicellular development proceeds normally in the mutant. During chemotaxis secG(-) cells elongate and migrate in a directed fashion towards cAMP, however speed is moderately reduced. The data indicate that SecG is a relevant factor for cell-substrate adhesion and reveal the basic function of a cytohesin in a lower eukaryote.

Transcript -- Dr Norman Cohen introduces four key characteristics any set of inherited instructions needs to have.

Dr Norman Cohen introduces four key characteristics any set of inherited instructions needs to have.

Transcript -- Dr Norman Cohen introduces four key characteristics any set of inherited instructions needs to have.

Dr Norman Cohen introduces four key characteristics any set of inherited instructions needs to have.

highwaybluespodcast2

Cytokinesis is the process that divides the cytoplasm of a parent cell into two. In animal cells, cytokinesis requires the formation of the central spindle and the contractile ring structures. The onset of cytokinesis is marked during anaphase with the specification of the division site, followed by cleavage furrow formation and ingression, midbody formation and abscission. The astral microtubules that originate from the centrosomes and the anti-parallel microtubules of the central spindle are proposed to determine the site of cleavage furrow formation (Bringmann and Hyman, 2005). The acto-myosin based contractile ring assembles at the division site and constricts the cytoplasm which is supported by the fusion of membrane vesicles to the ingressing plasma membrane. All these processes together result in the formation of two daughter cells. The small GTPase RhoA is one of the most upstream regulators of contractile ring assembly at the cortex. Rho proteins are activated by GEF’s (guanine nucleotide exchange factors) and one GEF that is required for cytokinesis is Ect2 (epithelial cell transforming protein2) (Tatsumoto et al., 1999). The Drosophila pebble (pbl) gene product is the founding member of the Ect2 protein family and has been shown to be required for cytokinesis (Lehner, 1992). In mammals, Ect2 was originally identified as a transforming protein in an expression cloning assay (Miki et al., 1993) and subsequently shown to be essential for cytokinesis. In this study, we have explored the temporal and spatial mechanisms that regulate Ect2 function. In agreement with previous studies, we show that Ect2 is a cell cycle regulated protein and is phosphorylated during mitosis. We identify a number of potentially interesting endogenous phosphorylation sites in Ect2, including potential Plk1 and Cdk1 sites. Although we have not been able to determine the function of these phosphorylation sites, their strong conservation among different species implies that they accomplish evolutionarily conserved roles.The identification of these phosphorylation sites sets the stage for future functional analyses. In complementary studies, we have shown that the central spindle and cell cortex localizations of Ect2 are facilitated by the BRCT and PH domains, respectively. The targeting of Ect2 to the central spindle is mediated by the MKlp1/MgcRacGAP and MKlp2/Aurora-B complexes. Of the two complexes, we show that Ect2 interacts and colocalizes only with the MKlp1/MgcRacGAP complex in telophase and propose that this interaction is mediated by a phosphorylation dependent docking mechanism that targets Ect2 to the central spindle. Interestingly, the displacement of Ect2 from the central spindle did not prevent cytokinesis, suggesting that localized GEF activity is not absolutely essential for cleavage furrow ingression and cytokinesis. In the second part of this thesis, we have explored the role of Ect2 during cytokinesis and show that, in Ect2 depleted cells, levels of RhoA and Citron kinase are diminished at the cleavage site, concomitant with the impairment of cleavage furrow formation and ingression. Additionally, overexpression of appropriate amino-terminal Ect2 fragments in cells also hinders cytokinesis. In these cells, RhoA and Citron kinase localize to the cortex and cleavage furrow ingression occurs, but, the subsequent abscission fails. Taken together, these results suggest that proper function of Ect2 is not only important for cleavage furrow ingression, but also for cell abscission. Finally, we investigate the overexpression phenotypes of different Ect2 truncation mutants. We show that abscission failure correlates with the persistence of amino-terminal Ect2 fragments at striking ring-like structures surrounding the midbody, indicating that completion of cell division requires the displacement of Ect2 from the contractile ring and its re-import into the reforming cell nucleus. Collectively, our data indicate that multiple mechanisms cooperate to regulate Ect2 in a spatial-temporal manner.

Fri, 15 Jan 1993 12:00:00 +0100 https://epub.ub.uni-muenchen.de/8828/1/kinetic_and_structural_analysis_of_the_mg2plus-binding_site_8828.pdf Wittinghofer, A.; Goody, R. S.; Borasio, Gian Domenico; Vetter, I.; Schlichting, I.; Rensland, H.; John, J.

G-Proteins are membrane-bound heterotrimeric polypeptides that couple receptor signals to second messenger systems such as cAMP. Recently, point mutations at 2 codons of the highly preserved alpha-chain of Gs, the adenyl cyclase-stimulating G-protein, were found in GH-secreting pituitary tumors. These mutations resulted in constitutively activated Gs alpha and high intracellular cAMP levels. In addition, point mutations at similar codons of a different G-protein, G(i) alpha 2, were reported in adrenocortical neoplasms, suggesting a potential role of this isoform in the genesis of these tumors. We reevaluated the frequency of constitutively activating point mutations in the alpha- chain of the stimulatory (Gs alpha) and inhibitory (G(i) alpha 2) G- proteins in human adrenocortical tumors. Seven adrenocortical carcinomas, 2 human adrenocortical tumor cell lines, and 11 adrenocortical adenomas were studied. Genomic DNA was purified from either frozen tumor tissue or paraffin-embedded sections. Using specific primers and the polymerase chain reaction, DNA fragments surrounding codons 201 and 227 (Gs alpha) and 179 and 205 (G(i) alpha 2) were amplified and visualized on a 2% agarose gel. In a second asymmetric polymerase chain reaction, using nested primers, single stranded DNA was generated using 1-10 microL of the initial amplification mixture and directly sequenced using the dideoxy chain termination method of Sanger. We found no mutations at codons 201, 227 and 179, 205 of Gs alpha and G(i) alpha 2, respectively, in the tumors studied. We conclude that previously identified oncogenic point mutations in the stimulatory and inhibitory alpha-chain of G-proteins do not appear to be present at high frequency in adrenal neoplasms. Thus, the mechanism(s) of tumorigenesis in these tumors is different from that in GH-secreting adenomas and may involve oncogenic mutations of other cell constituents.