Podcasts about endothelial

Endothelial cells form the inner lining of blood vessels, providing a smooth surface for efficient blood flow. Spanning approximately 60,000 miles of vessels, these specialized cells regulate circulation, immune function, inflammation, and metabolism. They also control vascular tone by producing nitric oxide (NO) and other signaling molecules, ensuring proper cardiovascular function and stability.This week George discusses the impact of endothelial cells and how nutrients like Vitamin E affect them.www.georgebatista.com

In this week's episode we'll learn about asciminib plus dasatinib in Philadelphia chromosome-positive acute leukemia. In a phase 1 study, combining two tyrosine kinase inhibitors with distinct mechanisms of action had encouraging activity in patients with de novo disease. After that: new research demonstrates that the iron exporter ferroportin contributes to the iron-sensing properties of liver endothelial cells. But is it the primary mediator of systemic iron homeostasis? Finally: anemia in pregnant patients with beta-thalassemia minor. In the third trimester, about one-third of patients have hemoglobin levels of 9 grams per deciliter or lower. This report may help to guide the appropriate diagnosis of anemia while limiting unnecessary testing and interventions. Featured Articles:Asciminib plus dasatinib and prednisone for Philadelphia chromosome–positive acute leukemiaThe hepcidin-ferroportin axis modulates liver endothelial cell BMP expression to influence iron homeostasis in miceβ-Thalassemia minor is associated with high rates of worsening anemia in pregnancy

In this episode, we go through the interplay between the oral microbiome, the intestinal microbiome, and brain health, focusing on the mechanisms through which dysbiosis in these ecosystems influences neuroinflammation and central nervous system function. We explore the molecular pathways by which bacterial metabolites and inflammatory mediators from the oral and intestinal microbiota can disrupt the blood-brain barrier, activate glial cells, and impair neuronal processes. We also go through symptoms associated with microbial imbalance as well as oral health practices. Topics: 1. Introduction Focus on neuroinflammation, oral microbiome, and intestinal microbiome Oral-gut-brain axis. 2. Neuroinflammation Overview Inflammatory response within the CNS. Key cell types: Endothelial cells, neurons, glial cells (microglia, astrocytes, oligodendrocytes). Acute vs. chronic neuroinflammation and their implications. 3. Cellular Mechanisms Microglial activation and its role in cytokine and ROS production. Astrocytic contributions to neuroinflammation. 4. Effects of Neuroinflammation on the Brain Mitochondrial dysfunction and energy deficits in neurons. Blood-brain barrier (BBB) disruption and immune cell infiltration. Impacts on synaptic function and neuronal communication. Potential contributors - environmental toxins, sleep deprivation, microbial dysbiosis, infections, and more. 5. Role of the Intestinal Microbiome in Neuroinflammation Dysbiosis and the release of LPS by Gram-negative bacteria. LPS transport across the intestinal barrier (transcellular and paracellular pathways). Systemic inflammation and its impact on BBB integrity and CNS inflammation. 6. The Oral Microbiome and Brain Health Direct effects of oral dysbiosis on systemic inflammation. Indirect effects through oral-gut communication and intestinal dysbiosis. 7. Trigeminal Nerve and Oral Dysbiosis Role of the trigeminal nerve in sensory transmission. Interaction of oral bacteria with the trigeminal nerve via TLRs and LPS recognition. 8. Symptoms and Effects of Oral Dysbiosis Common symptoms Systemic effects on the intestinal microbiome and brain health. 9. Lifestyle Practices Oral health practices. 10. Conclusion Summary of the oral-gut-brain axis and its role in neuroinflammation. Importance of oral health in reducing inflammatory burdens. Thank you to our episode sponsor: 1. Check out Ulyana Organics' Tallow Wild Yam Cream and Healing Facial Oil, and use code CHLOE10 10% off your order. Thanks for tuning in! Get Chloe's Book Today! "⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠75 Gut-Healing Strategies & Biohacks⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠" Follow Chloe on Instagram ⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠@synthesisofwellness⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠ Follow Chloe on TikTok @chloe_c_porter Visit ⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠synthesisofwellness.com⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠ to purchase products, subscribe to our mailing list, and more! --- Support this podcast: https://podcasters.spotify.com/pod/show/chloe-porter6/support

BUFFALO, NY- December 4, 2024 – A new #research paper was #published in Aging (listed by MEDLINE/PubMed as "Aging (Albany NY)" and "Aging-US" by Web of Science) Volume 16, Issue 21 on November 22, 2024, entitled, “The effects of young and aged, male and female megakaryocyte conditioned media on angiogenic properties of endothelial cells.” Researchers Murad K. Nazzal, Hanisha L. Battina, Nikhil P. Tewari, Sarah L. Mostardo, Rohit U. Nagaraj, Donghui Zhou, Olatundun D. Awosanya, Saveda K. Majety, Sue Samson, Rachel J. Blosser, Ushashi C. Dadwal, Patrick L. Mulcrone, and Melissa A. Kacenaat from Indiana University School of Medicine and Richard L. Roudebush Veterans' Administration Medical Center, have uncovered how certain bone marrow cells, called megakaryocytes (MKs), promote the growth of new blood vessels (angiogenesis) to aid in bone healing. Their findings help explain why healing slows with age and offer insights into potential treatments to accelerate fracture recovery in older adults. Broken bones are common in older adults, and slower healing can lead to complications and longer hospital stays. Accelerating the healing process could significantly improve the quality of life for millions. In this study, researchers investigated the effects of substances secreted by MKs, collected from young and older male and female mice. These substances, known as conditioned media (CM), were tested for their ability to stimulate the growth and function of endothelial cells (EC), which form the building blocks of blood vessels. Blood vessels play a critical role in healing by delivering oxygen and nutrients to damaged areas, making angiogenesis a vital part of the recovery process. The results showed that CM from younger MKs mice was more effective at helping blood vessels grow. Interestingly, MKs from female mice performed better than those from males, regardless of age. For example, substances from female MKs mice boosted blood vessel growth by over 115% and significantly improved the movement of cells needed for healing. The researchers also studied changes in genes related to blood vessel growth, and found that aging affects how these genes work. These changes may explain why older people heal more slowly after breaking a bone. “An understanding of which factors regulate which mechanisms of EC functionality may allow for isolation of one or a few factors that influence EC migration changes with aging, resulting in the development of targeted therapy to improve EC migration, subsequent angiogenesis, and fracture healing.” In conclusion, this research paves the way for developing new therapies to help older individuals recover from fractures more quickly, reducing pain and improving mobility. One potential approach could involve creating treatments that replicate the effects of MKs from younger individuals or isolating the specific substances that promote blood vessel growth. This represents an important step toward addressing the growing challenge of delayed healing in an aging population. DOI - https://doi.org/10.18632/aging.206077 Corresponding authors - Patrick L. Mulcrone - pamulcro@iu.edu, and Melissa A. Kacena - mkacena@iupui.edu About Aging-US The mission of the journal is to understand the mechanisms surrounding aging and age-related diseases, including cancer as the main cause of death in the modern aged population. The journal aims to promote 1) treatment of age-related diseases by slowing down aging, 2) validation of anti-aging drugs by treating age-related diseases, and 3) prevention of cancer by inhibiting aging. (Cancer and COVID-19 are age-related diseases.) Please visit our website at https://www.Aging-US.com​​. MEDIA@IMPACTJOURNALS.COM

Click here for Nitric Oxide products https://n1o1.com/ and use “Peakhuman” as a coupon code for 10% off!   Did you know that a tiny molecule could have a monumental impact on your health and well-being? Nitric oxide (NO) might be the unsung hero in your body, influencing everything from blood flow and exercise performance to disease prevention and recovery.But what makes this molecule so powerful?   In this episode of the Peak Human Labs Podcast, Dr. Sanjeev Goel speaks with Dr. Nathan Bryan, a leading expert in nitric oxide physiology. They explore NO's crucial role in human health, including its impact on blood flow, circulation, and oxygen delivery. Dr. Bryan explains the two primary pathways of NO production: enzymatic synthesis from L-arginine and dietary nitrates converted by oral bacteria. They also discuss how diet, oral health, and medications like proton pump inhibitors influence NO levels. Additionally, Dr. Bryan introduces innovative NO supplementation strategies, highlighting their potential benefits for cardiovascular health and disease prevention.   Tune in to discover how this remarkable molecule could be the key to enhancing your health. Key Takeaways   Importance of nitric oxide (NO) in human health Mechanisms of nitric oxide production in the body Pathways of nitric oxide synthesis, including the role of nitric oxide synthase (NOS) enzymes Impact of diet and oral bacteria on nitric oxide levels Implications of nitric oxide deficiency on various health conditions Effects of dietary choices, particularly nitrates from vegetables, on nitric oxide production Relationship between endothelial function and nitric oxide Nitric oxide supplementation strategies and their effectiveness Pharmacokinetics of nitric oxide delivery methods Potential therapeutic applications of nitric oxide in treating diseases like ischemic heart disease and Alzheimer's disease In This Episode:   [01:25] Dr. Bryan's background [03:13] Significance of nitric oxide [03:30] Misconceptions about nitric oxide [06:29] Mechanisms of nitric oxide production [08:20] Dietary influence on nitric oxide [09:34] Impact of oral bacteria [18:26] Antacids and nitric oxide deficiency [21:40] Young people and nitric oxide deficiency [22:55] Endothelial function and glycocalyx [24:17] Nitric oxide supplements [26:13] Introduction to nitric oxide [26:51] Mechanism of action [28:00] Restoring oral microbiome [29:01] Saliva and nitric oxide production [29:30] Dosage and frequency recommendations [31:25] Timing for optimal effects [32:22] Synergistic effects with medications [33:40] Tolerance and nitric oxide depletion [34:18] Details about Dr. Bryan's nitric oxide products and their benefits. [40:09] Conclusion and resources to learn more about nitric oxide.   Our Guest Dr. Nathan Bryan earned his B.S. in Biochemistry from the University of Texas at Austin and his M.D. from Louisiana State University School of Medicine, where he received the Dean's Award for Excellence in Research. He completed post-doctoral training as a Kirschstein Fellow at Boston University's Whitaker Cardiovascular Institute. Recruited by Nobel Laureate Ferid Murad to the University of Texas Health Science Center at Houston, Dr. Bryan has pioneered nitric oxide research for 20 years, resulting in numerous patents and significant advancements in patient care. He is the Founder and CEO of Bryan Therapeutics, Inc., leading in nitric oxide-based therapies for various conditions.   Resources and Links Peak Human Labs https://www.youtube.com/@peakhumanlabs/videos https://www.peakhuman.ca/ https://www.instagram.com/peakhumanlabs/?hl=en https://open.spotify.com/show/5hx9R37ElxgzCrBccRWoHd?si=8atK0n82QbeL3DWg5-vjvg&nd=1&dlsi=ce0f77aa4f304724 Dr. Sanjeev Goel https://www.linkedin.com/in/sanjeevgoelmd/?originalSubdomain=ca Dr. Nathan Bryan https://drnathansbryan.com/ https://www.linkedin.com/in/nathan-bryan-27586b7/ https://www.youtube.com/results?search_query=dr+nathan+s+bryan https://web.facebook.com/humann/?_rdc=1&_rdr

In this episode of the Healthy CEO Podcast, hosted by Jason Wright and Dr. Troy Jackson and sponsored by Authentic Health Partners, the focus is on the critical aspects of cardiovascular health, specifically endothelial health. Dr. Troy and Jason discuss the importance of small habit changes, the benefits of incremental improvements in diet and exercise, and the role of supplements like green seaweed extract and antioxidants. They explore the potential need for medications such as statins and metformin in certain cases and emphasize personalized health interventions amidst modern environmental challenges. The conversation underscores the significance of antioxidants, polyphenols, dietary nitrates, and fiber, while warning against the overconsumption of saturated fats. Practical advice, real-life examples, and personal anecdotes provide listeners with actionable strategies to enhance their cardiovascular health.00:00 Starting Small: The Key to Habit Change00:42 Welcome to the Healthy CEO Podcast01:11 The Silent Killer: Cardiovascular Health02:41 Understanding Endothelial Health04:04 Proactive Steps for Better Health04:52 Recap of Part One: Setting the Stage09:24 Dietary Patterns for Heart Health11:50 The Importance of Fruits and Vegetables13:56 Debunking Myths About Fruits and Fructose25:04 The Role of Fat in Cardiovascular Health35:55 The Benefits of Extra Virgin Olive Oil41:16 The Importance of Fiber49:44 Smoothie Hacks and Bone Broth Benefits50:35 The Power of Exercise52:16 Understanding Zone Training55:28 Encouragement and Long-Term Health Goals01:07:00 Supplements for Endothelial Health01:20:50 The Role of Medications01:28:02 Final Thoughts and Encouragement

Endothelial health refers to the well-being of the endothelium, the thin layer of cells lining the interior surface of blood vessels. This layer plays a crucial role in vascular function, including regulating blood flow, preventing clotting, and maintaining the balance of substances between the bloodstream and surrounding tissues. Maintaining endothelial health is vital for longevity as it ensures optimal blood circulation, reduces the risk of cardiovascular diseases, and supports overall vascular function. Healthy endothelium helps prevent atherosclerosis, hypertension, and other conditions that can lead to heart attacks, strokes, and other serious health issues. Through lifestyle choices like a balanced diet, regular exercise, and stress management, one can promote endothelial health and enhance longevity.Dr. Troy Jackson loves his role as a family medicine physician to develop lasting relationships with his patients while encouraging and guiding them along their journey to a better self. He has a particular interest in genomics-based personalized medicine, but Dr. Jackson finds all aspects of primary care fascinating. After growing up in central Mississippi and attending The University of Mississippi for both his undergraduate and medical school educations, Dr. Jackson completed his family medicine residency at the MAHEC Family Medicine Residency Program here in Asheville, NC.

Endothelial health refers to the well-being of the endothelium, the thin layer of cells lining the interior surface of blood vessels. This layer plays a crucial role in vascular function, including regulating blood flow, preventing clotting, and maintaining the balance of substances between the bloodstream and surrounding tissues. Maintaining endothelial health is vital for longevity as it ensures optimal blood circulation, reduces the risk of cardiovascular diseases, and supports overall vascular function. Healthy endothelium helps prevent atherosclerosis, hypertension, and other conditions that can lead to heart attacks, strokes, and other serious health issues. Through lifestyle choices like a balanced diet, regular exercise, and stress management, one can promote endothelial health and enhance longevity.Dr. Troy Jackson loves his role as a family medicine physician to develop lasting relationships with his patients while encouraging and guiding them along their journey to a better self. He has a particular interest in genomics-based personalized medicine, but Dr. Jackson finds all aspects of primary care fascinating. After growing up in central Mississippi and attending The University of Mississippi for both his undergraduate and medical school educations, Dr. Jackson completed his family medicine residency at the MAHEC Family Medicine Residency Program here in Asheville, NC.

AHK-Cu, also known as Copper Tripeptide-1, has garnered significant attention in dermatology and cosmetology for its many benefits. The cosmeceutical industry has studied it extensively in animal and lab models.  This short peptide, consisting of the amino acids alanine, histidine, and lysine chemically bound to a copper ion, plays a crucial role in skin health, collagen synthesis, and hair growth.  In this podcast, we'll examine how AHK-Cu works and its potential side effects. How does it work? AHK-Cu works in many ways to help with skin health, collagen synthesis, and hair growth. Skin Health: AHK-Cu can increase VEGF (vascular endothelial growth factor) levels. VEGF is a protein found in the body that can make new blood cells when your body needs them. By increasing VEGF levels, AHK-Cu can activate fibroblasts and endothelial cells. This is a good thing because fibroblasts produce collagen and elastin, two molecules that help with skin tone, texture, and flexibility. Endothelial cells line blood vessels and help with blood vessel health and growth. Increased levels of fibroblasts and endothelial cells help improve wound healing and decrease fine lines and wrinkles.  Collagen Synthesis: Collagen is a vital protein that provides structural support to the skin, maintaining its firmness and elasticity. As we mentioned, AHK-Cu boosts collagen synthesis by activating fibroblasts, the cells responsible for producing collagen. The copper ion in AHK-Cu is essential for the cross-linking of collagen and elastin fibers, which helps maintain the skin's structural integrity and reduces the appearance of fine lines and wrinkles. Also, keep in mind that collagen attracts water. This is important because when water enters the skin, it hydrates the cells of the extracellular matrix, which helps reduce the appearance of lines and wrinkles.  Hair Growth: AHK-Cu promotes hair growth in two ways. First, it encourages the growth of blood vessels by stimulating the secretion of VEGF. This is important because blood vessels are needed to supply nutrients to the hair follicles. AHK-Cu also decreases dihydrotestosterone (DHT), the hormone that's involved in male pattern baldness and thinning hair for both men and women as they age.  What are the potential benefits of AHK-Cu? Improved Skin Appearance: Regular use of AHK-Cu can significantly improve skin texture, tone, and overall appearance. It helps reduce signs of aging, such as wrinkles and fine lines and enhances skin firmness and elasticity. Promotes Wound Healing: AHK-Cu accelerates wound and scar healing by promoting cellular repair and reducing inflammation. This makes it an effective ingredient in post-procedure skincare products and treatments for conditions like acne scars. Hair Growth: For individuals experiencing hair thinning or hair loss, AHK-Cu can be a valuable addition to their hair care regimen. It promotes thicker, healthier hair by stimulating the hair follicles and prolonging the hair growth phase. Keep an eye out for these AHK-Cu side effects. While AHK-Cu is generally considered safe and well-tolerated, it is important to be aware of potential side effects: Skin Irritation: Some individuals may experience mild skin irritation, redness, or itching when using products containing AHK-Cu, especially if they have sensitive skin or are using it for the first time. Allergic Reactions: Although rare, allergic reactions to AHK-Cu can occur. Symptoms may include rash, itching, hives, and trouble breathing. To rule out allergic reactions, a patch test should be performed before using a new product containing AHK-Cu. Discoloration: Due to its copper content, AHK-Cu can cause temporary blue or green skin discoloration in some cases. This is typically harmless and fades with continued use or once the product is washed off. Proper use of AHK-Cu can significantly enhance skin vitality and hair density, offering a natural and effective solution to common cosmetic concerns. Thanks again for listening to The Peptide Podcast. We love having you as part of our community. If you love this podcast, please share it with your friends and family on social media, and have a happy, healthy week! We're huge advocates of elevating your health game with nutrition, supplements, and vitamins. Whether it's a daily boost or targeted support, we trust and use Momentous products to supercharge our wellness journey.  Momentous only uses the highest-quality ingredients, and every single product is rigorously tested by independent third parties to ensure their products deliver on their promise to bring you the best supplements on the market.  WHAT TO LISTEN TO NEXT: Losing hair? Hair loss may be preventable. Listen to the hair growth and peptide therapy podcast. Want healthier skin and hair? Learn about copper peptides for skin and hair health. GLP-1's and hair loss: Hair loss isn't directly caused by GLP-1's. Listen to find out what causes hair loss when you take a weight loss medication.

BUFFALO, NY- April 30, 2024 – A new #researchpaper was #published on the #cover of Aging (listed by MEDLINE/PubMed as "Aging (Albany NY)" and "Aging-US" by Web of Science) Volume 16, Issue 8, entitled, “Senescent characteristics of human corneal endothelial cells upon ultraviolet-A exposure.” In this new study, researchers Kohsaku Numa, Sandip Kumar Patel, Zhixin A. Zhang, Jordan B. Burton, Akifumi Matsumoto, Jun-Wei B. Hughes, Chie Sotozono, Birgit Schilling, Pierre-Yves Desprez, Judith Campisi (1948-2024), and Koji Kitazawa from Buck Institute for Research on Aging, Kyoto Prefectural University of Medicine, University of Cambridge, and California Pacific Medical Center investigated the senescent phenotypes of human corneal endothelial cells (hCEnCs) upon treatment with ultraviolet (UV)-A. “We assessed cell morphology, senescence-associated β-galactosidase (SA-β-gal) activity, cell proliferation and expression of senescence markers (p16 and p21) in hCEnCs exposed to UV-A radiation, and senescent hCEnCs induced by ionizing radiation (IR) were used as positive controls.” The researchers performed RNA sequencing and proteomics analyses to compare gene and protein expression profiles between UV-A- and IR-induced senescent hCEnCs — they also compared the results to non-senescent hCEnCs. Cells exposed to 5 J/cm2 of UV-A or to IR exhibited typical senescent phenotypes, including enlargement, increased SA-β-gal activity, decreased cell proliferation and elevated expression of p16 and p21. RNA-Seq analysis revealed that 83.9% of the genes significantly upregulated and 82.6% of the genes significantly downregulated in UV-A-induced senescent hCEnCs overlapped with the genes regulated in IR-induced senescent hCEnCs. Proteomics also revealed that 93.8% of the proteins significantly upregulated in UV-A-induced senescent hCEnCs overlapped with those induced by IR. In proteomics analyses, senescent hCEnCs induced by UV-A exhibited elevated expression levels of several factors part of the senescence-associated secretory phenotype. “In this study, where senescence was induced by UV-A, a more physiological stress for hCEnCs compared to IR, we determined that UV-A modulated the expression of many genes and proteins typically altered upon IR treatment, a more conventional method of senescence induction, even though UV-A also modulated specific pathways unrelated to IR.” DOI - https://doi.org/10.18632/aging.205761 Corresponding author - Koji Kitazawa - kkitazaw@koto.kpu-m.ac.jp Sign up for free Altmetric alerts about this article - https://aging.altmetric.com/details/email_updates?id=10.18632%2Faging.205761 Subscribe for free publication alerts from Aging - https://www.aging-us.com/subscribe-to-toc-alerts About Aging-US Aging publishes research papers in all fields of aging research including but not limited, aging from yeast to mammals, cellular senescence, age-related diseases such as cancer and Alzheimer's diseases and their prevention and treatment, anti-aging strategies and drug development and especially the role of signal transduction pathways such as mTOR in aging and potential approaches to modulate these signaling pathways to extend lifespan. The journal aims to promote treatment of age-related diseases by slowing down aging, validation of anti-aging drugs by treating age-related diseases, prevention of cancer by inhibiting aging. Cancer and COVID-19 are age-related diseases. Please visit our website at https://www.Aging-US.com​​ and connect with us: Facebook - https://www.facebook.com/AgingUS/ X - https://twitter.com/AgingJrnl Instagram - https://www.instagram.com/agingjrnl/ YouTube - https://www.youtube.com/@AgingJournal LinkedIn - https://www.linkedin.com/company/aging/ Pinterest - https://www.pinterest.com/AgingUS/ Spotify - https://open.spotify.com/show/1X4HQQgegjReaf6Mozn6Mc MEDIA@IMPACTJOURNALS.COM

The current thoughts around LDL cholesterol center on the fact that there is no scenario in which high LDL numbers could occur in healthy individuals. Traditionally, the belief is high levels of LDL can lead to a greater risk of cardiovascular disease regardless of metabolic health. Today's guest is here to share his hypothesis and research on how higher cholesterol levels in metabolically fit individuals could be a physiological response rather than a pathological response that can lead to disease. Today on The Dhru Purohit Show, Dhru sits down with Dave Feldman to discuss high levels of LDL in metabolically healthy individuals. Dave shares the current research on high LDL and cardiovascular disease in individuals eating a ketogenic diet. He also shares his personal approach to focusing on metabolic health and the key markers critical to overall health. Dave discusses the process and findings of the Oreo experiment conducted by his colleague and explains why this experiment further proves their hypothesis on how LDL particles move through the body. Dave Feldman is a software and platform engineer, entrepreneur, and founder of the Citizen Science Foundation. Through a series of self-experiments and partnering with formal researchers, he has since published the "Lipid Energy Model," which may explain this phenomenon. In this episode, Dhru and Dave dive into (audio version / Apple Subscriber version):Dave's hypothesis on LDL (00:24 /00:24)Rethinking our approach to high LDL (2:17 / 2:17) The research on high LDL and cardiovascular disease (10:00 / 6:48) The Oreo experiment and what it shows about fat adaptation (17:23 / 13:45)The pushback to lean mass hyper responder (27:36 / 24:15) Soft plaque versus hard plaque and the risk of cardiovascular disease (37:50 / 35:04) Dave's personal approach in focusing on overall metabolic health (51:42 / 48:30) Fasting Insulin and Vitamin D (59:33 / 53:45) Endothelial health and cardiovascular health (1:03:28 / 57:45) The vegan's twin study and the correlation to LDL (1:21:17 / 1:15:49)Dhru's experience (1:32:17 / 1:26:36) Also mentioned in this episode:Citizen Science Foundation Oreo Cookie TreatmentNicolas Norwitz, PhD Twitter This episode is brought to you by LMNT, Momentous, and Lumebox. Right now, LMNT is offering my listeners a free sample pack with any purchase. Head over to DrinkLMNT.com/dhru today.Optimize your Omega-3 levels by choosing a quality fish oil made by and used by the best. Go to livemomentous.com and enter promo code DHRU to get 20% off any order. Lumebox is offering my community $260 off their FDA-approved portable Red Light device! That's over 50% off! Go to thelumebox.com/dhru and get your Red Light device. Hosted on Acast. See acast.com/privacy for more information.

In this enlightening conversation, Dr. geo interviews Dr. Miles Spar, a renowned clinical practice leader focusing on chronic diseases and integrative medicine. Dr. Spar brings his knowledge to the table, highlighting the critical yet underappreciated role of endothelial cells and the endothelial glycocalyx in maintaining arterial health. Discover the vital connections between our daily lifestyle choices and the health of our arterial lining, and learn how these factors contribute to the prevention of heart attacks, the enhancement of male sexual health, and the overall well-being of the cardiovascular system. Dr. Spar emphasizes the destructive impact of poor diet and lack of exercise on our endothelial health and offers actionable advice on nurturing this essential component of our arteries.Furthermore, we delve into the significance of nitric oxide in regulating blood flow and explore the promising effects of marine polysaccharides on the endothelial glycocalyx. Dr. Spar also discusses innovative measurement tools and biomarkers, such as lipoprotein and CRP levels, providing listeners with insights into monitoring and improving their arterial health.___________Dr. Spar's website: DrSpar.comTwitter: @DrSparInsta: @DrSparFB: @Myles Spar, MDLinkedIn: @DrSpar___________Thank you to our sponsors.This episode is brought to you by CALROY Health Sciences, our proud sponsor for the HEART SERIES this February, Heart Health Month, on the Dr. Geo Podcast. CALROY is at the forefront of heart wellness with its premier dietary supplements, Arterosil HP® & Vascanox HP®, designed to support cardiovascular health. Arterosil HP® is essential for enhancing the endothelial glycocalyx and crucial for heart and vascular system efficiency. Vascanox HP®, on the other hand, boosts nitric oxide production, which is essential for a healthy heart and vascular system. Together, they're the ultimate duo for vascular support. Join us in exploring these heart health essentials with CALROY Health Sciences.This episode is also brought to you by AG1 (Athletic Greens). AG1 contains 75 high-quality vitamins, minerals, whole-food sourced ingredients, probiotics, and adaptogens to help you start your day right. This unique blend of ingredients supports your gut health, nervous system, immune system, energy, recovery, focus, and aging. All the things. Enjoy AG1 (Athletic Greens).----------------Thanks for listening to this week's episode. Subscribe to The Dr. Geo YouTube Channel to get more content like this and learn how to live better with age.You can also listen to this episode and future episodes of the Dr. Geo Podcast by clicking HERE----------------Follow Dr. Geo on social media. Facebook , Instagram Click here to join Dr. Geo's Health Community.Improve your urological health with Dr. Geo's formulated supplement lines:

Endothelial keratoplasty (EK) procedures have become the preferred method of corneal transplantation in the United States. As the frequency of EKs increase, so do associated costs. Dr. Matt Feng interviews Drs. David S. Portney and Shahzad I. Mian about their recent analysis to assess for differences in surgical costs and surgery length based on type of EK, use of preloaded grafts, and performance of simultaneous cataract surgery with EK from their Ophthalmology article “Cost Drivers of Endothelial Keratoplasty: A Time-Driven Activity-Based Costing Analysis.” Cost Drivers of Endothelial Keratoplasty. Goldstein, Jenna K. et al. Ophthalmology, Volume 130, Issue 10, 1073 – 1079. Sign up for the next Ophthalmology Journal Virtual Club on March 6, 2024, at https://store.aao.org/ophthalmology-virtual-journal-club.html

How can we improve the capacity of our blood vessels to repair themselves?

BUFFALO, NY- December 27, 2023 – A new #research paper was #published in Aging (listed by MEDLINE/PubMed as "Aging (Albany NY)" and "Aging-US" by Web of Science) Volume 15, Issue 23, entitled, “Angelica gigas extract inhibits acetylation of eNOS via IRE1α sulfonation/RIDD-SIRT1-mediated posttranslational modification in vascular dysfunction.” Angelica gigas NAKAI (AG) is a popular traditional medicinal herb widely used to treat dyslipidemia owing to its antioxidant activity. Vascular disease is intimately linked to obesity-induced metabolic syndrome, and AG extract (AGE) shows beneficial effects on obesity-associated vascular dysfunction. However, the effectiveness of AGE against obesity and its underlying mechanisms have not yet been extensively investigated. In this new study, researchers Geum-Hwa Lee, Hwa-Young Lee, Young-Je Lim, Ji-Hyun Kim, Su-Jin Jung, Eun-Soo Jung, Soo-Wan Chae, Juwon Lee, Junghyun Lim, Mohammad Mamun Ur Rashid, Kyung Hyun Min, and Han-Jung Chae from Jeonbuk National University and Jeonbuk National University Hospital supplemented 40 high fat diet (HFD) rats with 100–300 mg/kg/day of AGE to determine its efficacy in regulating vascular dysfunction. “[...] the primary aim of this study is to examine the inhibitory effects of AGE on dyslipidemia-associated vascular dysfunction, with a focus on its potential mechanisms of action.” The vascular relaxation responses to acetylcholine were impaired in HFD rats, while the administration of AGE restored the diminished relaxation pattern. Endothelial dysfunction, including increased plaque area, accumulated reactive oxygen species, and decreased nitric oxide (NO) and endothelial nitric oxide synthase (eNOS) Ser1177 phosphorylation, were observed in HFD rats, whereas AGE reversed endothelial dysfunction and its associated biochemical signaling. Furthermore, AGE regulated endoplasmic reticulum (ER) stress and IRE1α sulfonation and its subsequent sirt1 RNA decay through controlling regulated IRE1α-dependent decay (RIDD) signaling, ultimately promoting NO bioavailability via the SIRT1-eNOS axis in aorta and endothelial cells. Independently, AGE enhanced AMPK phosphorylation, additionally stimulating SIRT1 and eNOS deacetylation and its associated NO bioavailability. Decursin, a prominent constituent of AGE, exhibited a similar effect in alleviating endothelial dysfunctions. These data suggest that AGE regulates dyslipidemia-associated vascular dysfunction by controlling ROS-associated ER stress responses, especially IRE1α-RIDD/sirt1 decay and the AMPK-SIRT1 axis. “Ultimately, this study presents clearly evidence that AGE is a promising natural product-based functional food/herbal medicine candidate for preventing or regulating hyperlipidemic cardiovascular complications.” DOI - https://doi.org/10.18632/aging.205343 Corresponding authors - Kyung Hyun Min - khmin1492@jbnu.ac.kr, and Han-Jung Chae - hjchae@jbnu.ac.kr About Aging-US Launched in 2009, Aging-US publishes papers of general interest and biological significance in all fields of aging research and age-related diseases, including cancer—and now, with a special focus on COVID-19 vulnerability as an age-dependent syndrome. Topics in Aging-US go beyond traditional gerontology, including, but not limited to, cellular and molecular biology, human age-related diseases, pathology in model organisms, signal transduction pathways (e.g., p53, sirtuins, and PI-3K/AKT/mTOR, among others), and approaches to modulating these signaling pathways. Visit https://www.Aging-US.com​​ and connect with us: SoundCloud - https://soundcloud.com/Aging-Us Facebook - https://www.facebook.com/AgingUS/ X - https://twitter.com/AgingJrnl Instagram - https://www.instagram.com/agingjrnl/ YouTube - https://www.youtube.com/@AgingJournal LinkedIn - https://www.linkedin.com/company/aging/ Pinterest - https://www.pinterest.com/AgingUS/ MEDIA@IMPACTJOURNALS.COM

Dr. Fardink shares the latest in eye care news and trends. Learn about Omega-3s on Diabetic Retinopathy, Smart Contact Lenses, Corneal Endothelial Cell Therapy, and More.

For this third and final episode recorded at ARM's 2023 Meeting on the Mesa, Cell & Gene: The Podcast Host, Erin Harris, sat down with Greg Kunst, president, CEO, and Board member at Aurion Biotech. Listen in as they discuss the cell therapy used to treat corneal endothelial disease and how Greg navigated the event as a first-timer attending Meeting on the Mesa.

Long COVID, also known as post-acute sequelae of SARS-CoV-2 infection (PASC) or long-haul COVID, refers to a condition where individuals experience persistent symptoms or develop new symptoms after recovering from the acute phase of COVID-19. Long COVID can affect individuals who had mild, moderate, or severe initial COVID-19 infections and can persist for weeks or months after the initial illness. The specific symptoms and their duration can vary widely between individuals, but common symptoms of long COVID include fatigue, shortness of breath, cough, joint pain, chest pain, muscle weakness, brain fog, difficulty concentrating, memory problems, sleep issues, depression, anxiety, and other neurological or psychiatric symptoms. It can also affect multiple organs in the body, such as the heart, lungs, kidneys, and brain. How does COVID-19 affect microcirculation? Microcirculation refers to the circulation of blood in the smallest blood vessels, including arterioles, capillaries, and venules. While COVID-19 primarily affects the respiratory system, there is evidence suggesting that it can have systemic effects, including impacts on the cardiovascular system and microcirculation. Here are some potential ways in which COVID-19 may affect microcirculation: Endothelial Dysfunction: COVID-19 has been associated with endothelial dysfunction, which is a condition where the cells lining blood vessels (endothelial cells) do not function properly. Endothelial dysfunction can lead to impaired regulation of blood flow and increased permeability of blood vessels. In severe cases, viral infection and the resulting immune response may damage endothelial cells, contributing to a pro-inflammatory state and a potential disruption of microcirculation. Blood Clotting and Thrombosis: COVID-19 is known to be associated with an increased risk of blood clot formation (thrombosis). The formation of blood clots can potentially affect microcirculation by blocking small blood vessels. The hypercoagulable state observed in some COVID-19 patients may contribute to microvascular thrombosis, leading to impaired blood flow in affected tissues. Inflammatory Response: The body's inflammatory response to the virus can also impact microcirculation. Inflammation can lead to the release of inflammatory mediators, causing vasodilation (widening of blood vessels) and increased permeability, which may affect blood flow in the microcirculation. Hypoxia and Tissue Damage: Severe cases of COVID-19 may lead to respiratory distress and hypoxia (low oxygen levels). Hypoxia can have detrimental effects on tissues and organs, potentially impacting microcirculation. Tissue damage and inflammation in the lungs may trigger a systemic response that affects microvascular function in other organs. Impaired Oxygen Delivery: In severe cases of COVID-19, where acute respiratory distress syndrome (ARDS) develops, oxygen exchange in the lungs becomes compromised. This can lead to inadequate oxygen delivery to tissues and affect microcirculation. What is grapeseed extract? Grapeseed extract is a dietary supplement derived from the seeds of grapes. It is rich in antioxidants, particularly compounds known as oligomeric proanthocyanidin complexes (OPCs). Additionally, grape seed extract contains flavonoids, another class of polyphenols with antioxidant properties. These antioxidants help protect the body against damage from harmful free radicals, which can play a role in various chronic diseases. Grapeseed extract is commonly used for its potential health benefits, including improved cardiovascular health, reduced inflammation, enhanced immune function, and anti-aging effects. How does grapeseed extract improve microcirculation in Long COVID? The proanthocyanidins in grape seed extract help improve blood flow and circulation. By promoting the dilation of blood vessels, the extract supports the cardiovascular system's ...

References J Diabetes Investig. 2023Oct;14(10):1148-1156 J Pediatr. 2021 Apr; 231: 200–206.e1. Front Physiol. 2020; 11: 796. ClinicaChimicaActa.2020 Volume 508, September Pages 61-68 Mozart,WA. 1787. Eine kleine nachtmusik K.525 https://youtu.be/nPbxIT9W1AY?si=mgzBQzpvuFzsVNtB --- Send in a voice message: https://podcasters.spotify.com/pod/show/dr-daniel-j-guerra/message Support this podcast: https://podcasters.spotify.com/pod/show/dr-daniel-j-guerra/support

Hey podcast community, Dr. Mark here. My team and I are so excited to offer you a 7 Day Free trial of the Dr. Hyman+ subscription for Apple Podcast. For 7 days, you get access to all this and more entirely for free! It's so easy to sign up. Just go click the Try Free button on the Doctor's Farmacy Podcast page in Apple Podcast. In this teaser episode, you'll hear a preview of our latest Dr. Hyman+ Functional Medicine Deep Dive on endothelial health with Dr. Michael Twyman.Learn more about Dr. Twyman at drtwyman.com and follow him on Instagram @drtwymanWant to hear the full episode? Subscribe now. With your 7 day free trial to Apple Podcast, you'll gain access to audio versions of:- Ad-Free Doctor's Farmacy Podcast episodes- Exclusive monthly Functional Medicine Deep Dives- Monthly Ask Mark Anything Episodes - Bonus audio content exclusive to Dr. Hyman+Trying to decide if the Dr. Hyman+ subscription for Apple Podcast is right for you? Email my team at plus@drhyman.com with any questions you have. Please note, Dr. Hyman+ subscription for Apple Podcast only includes Dr. Hyman+ in audio content. Hosted on Acast. See acast.com/privacy for more information.

If you're struggling with stiff or aching joints, and you're tired of letting this discomfort steal the joy and freedom from your life, then Joint Support by PureHealth Research is a natural solution you're going to love. It contains 7 of Mother Nature's best superfoods for supporting comfortable, healthy, and flexible joints.   All it takes is 1 small capsule of Joint Support every day to start feeling the positive effects on your joint health. It can promote healthy joint lubrication making it easier to move in comfort. Try Joint Support risk-free today and get a FREE 30-day supply of Omega 3 when you take advantage of this special offer. You are also getting 2 free E-Books, so you can learn more about joint health.   Head over to GetJointHelp.com/Jockers to order Joint Support and claim your free bottle of Omega 3 while supplies last. That's GetJointHelp.com/Jockers   Are hidden toxins and stressors making you feel rundown and tired? Are you worried about oxidative stress from exposure to EMF, 5G, heavy metals, chemicals, processed foods, and the like? Toxic is the 'new normal' in our modern world.    When left to roam free, toxins take on the form of free radicals in our bodies, causing oxidative damage on a cellular level (which can lead to premature aging and a range of health problems).    The good news is Glutathione, one of the world's most powerful antioxidants combats free radicals in our body while repairing DNA and flushing out toxins.   Purality Health has a patented formula that utilizes 'MICELLE LIPOSOMAL' technology which delivers the Glutathione antioxidant straight into your bloodstream and is proven to be 800% more efficient! It's also backed by a 180-day money-back guarantee.    Say goodbye to toxins in your body! Today, we have a 30% OFF coupon for you!  Visit puralityhealth.com and use the coupon "DRJ" to access 30% OFF!   What is the REAL reason we need to be drinking warm lemon water? Well, there are a few great ones actually, and in today's awesome aqua-focused episode of Functional Nutrition, Dr. Jockers is giving us the download on why lemon water is so good for you.   Did you know that just by adding a pinch of salt to your warm lemon water, it will act as an anti-histamine, thus helping with your allergies? This trick can also lower your cortisol levels too!   Learn how to improve your gut microbiome, your bowel movements, and your overall energy and digestion in this short and sweet (ok, slightly lemony) episode from our favorite holistic health practitioner, Dr. Jockers. It's time to activate your Vagus nerve for improved parasympathetic tone, don't you think?   Please join us. Please remember to leave a five-star review for the show if you enjoy our content. Thank you!   “Lemon is rich in Vitamin C as well as bioflavonoids. Citrus bioflavonoids. Those bioflavonoids synergize with Vitamin C and they help improve oxygen extraction and oxygen delivery into the cells." - Dr. David Jockers   Subscribe to the podcast on: Apple Podcast Stitcher Spotify PodBean TuneIn Radio In This Episode:   Starting with a warm drink early in the day How Vitamin C improves our Endothelial lining The power of electrolytes What is structured water? What does it do? Appreciating water as a communication system How lemon triggers our vagus nerve (and calms our body) How lemon's acidity balances our gut microbiome How can you heal your stomach lining? Adding salt to balance your electrolyte profile What happens if your cortisol level gets too high? How much salt should you add? What are the additional benefits of warm lemon water? Combining your warm lemon water with your intermittent fasting routine Resources: Puralityhealth.com - Use coupon DRJ to get 30% off your Glutathione Master Antioxidant today! Pure Health Research - Free bottle of Nano Powered Omega 3 when you buy Joint Support!     Connect with Dr. Jockers: Instagram – https://www.instagram.com/drjockers/ Facebook – https://www.facebook.com/DrDavidJockers YouTube – https://www.youtube.com/user/djockers Website – https://drjockers.com/ If you are interested in being a guest on the show, we would love to hear from you! Please contact us here! - https://drjockers.com/join-us-dr-jockers-functional-nutrition-podcast/

References Telemann Georg P. 1765. Overture D Major, TWV 55:D18 | Tafelmusik https://youtu.be/8r-IFLtN9x4?si=T2hp65oGT--dTivY Leukemia 2020 volume 34, pages 75–86 Nat Cell Biol. 2019 Mar; 21(3): 397–407 --- Send in a voice message: https://podcasters.spotify.com/pod/show/dr-daniel-j-guerra/message Support this podcast: https://podcasters.spotify.com/pod/show/dr-daniel-j-guerra/support

This episode is brought to you by BiOptimizers, Joovv, and Rupa Health.Today on The Dhru Purohit Podcast, Dhru sits down with Dr. Joel Kahn to discuss the different screening tests and biomarkers that can paint a clearer picture of heart health. Dr. Joel Kahn is a practicing cardiologist and a clinical professor of medicine at Wayne State University School of Medicine. He's triple-board-certified in internal medicine, cardiovascular medicine, and interventional cardiology.In this episode, Dhru and Dr. Kahn dive into (audio version / Apple Subscriber version):-How to truly know if your heart is healthy (2:16 / 2:16)-The various blood and metabolic biomarkers for testing cardiac function (4:00 / 4:00)-The Cleerly scan for measuring soft plaque (17:47 / 15:16)-Dr. Kahn's recommendations for HIIT and resistance training (25:15 / 22:33)-Lifestyle tips for reducing soft plaque build-up (34:20 / 30:24)-Endothelial health, nitric oxide production, and the risk for heart disease (40:50 / 36:51)-What specific biomarkers can tell us about heart disease risk and the role of personalized medicine for effective treatment interventions (45:36 / 41:39)-Supplements to aid in lowering soft plaque levels (53:57 / 50:00)-Genetic testing for assessing heart disease risk (1:02:46 / 58:48)-Dr. Kahn's thoughts on dietary protein and resistance training for longevity (1:15:31 / 1:11:58)-What Dr. Kahn does daily to support his longevity (1:31:30 / 1:28:00)Also mentioned in this episode:-Kahn Center for Cardiac Longevity-Heart Doc VIP Dr. Joel Kahn's podcast-What Do I Do With My High Coronary Calcium CT Results: A Guide-HIIT Training Improves Soft Plaque (study)-Try This: VO2 Max as the Best Predictor of Longevity-GB Insight Genetic Testing and Analysis-Maxi Health Kyolic 400 Aged Garlic Extract -Gotu Kola and Pycnogenol French Maritime Pine Bark ExtractsBiOptimizers is offering 10% off plus a special gift with purchase. Just head over to magbreakthrough.com/dhru with code DHRU10.Right now, Joovv is giving my listeners an exclusive offer on your first order. Head over to Joovv.com/dhru and apply my code DHRU.Access more than 3,000 specialty lab tests with Rupa Health. You can check out a free, live demo with a Q&A or create an account at RupaHealth.com today. Hosted on Acast. See acast.com/privacy for more information.

Watch the full video interview on YouTube here: https://bit.ly/555drnathanbryan Dr. Nathan Bryan (IG: @drnathansbryan) is a nitric oxide scientist, entrepreneur and author. He's published a number of highly cited papers. In this episode, we discuss: What is nitric oxide (NO)? The two pathways for nitric oxide production Supplemental arginine is not the NO solution Endothelial dysfunction increases as you age The top 3 things that disrupt nitric oxide production Organic produce has less nitrates than conventional The best advice for buying produce Why soil sampling is important Why you need to stop using mouthwash today! The American lifestyle shuts down nitric oxide production Fix your oral microbiome in 4 days Does tongue scraping affect heart health? The problem with antacids How to wean off acid reflux medications Should you take digestive enzymes? Glutathione and nitric oxide How long does nitric oxide impact the body? Dosing of nitrates Two signs of nitric oxide toxicity Nitric oxide testing options Debunking the myth that nitrites in meat cause cancer The history of meat curing Is beef a good source of nitrates? Nathans's thoughts on plant toxins A MTHFR gene mutation limits nitric oxide production Human optimization defined The difference between nitriceuticals and nutraceuticals Nathan created a product that fixes endothelial dysfunction The truth about erectile dysfunction and Viagra Erectile dysfunction is a warning you NEED to pay attention to The benefits of sunlight and exercise for nitric oxide Show sponsor: Quicksilver Scientific

Diabetic macular oedema is a common complication of diabetes, in which damage to the blood vessels at the back of the eye leads to swelling. Lucentis, Eylea and Avastin are three antiangiogenic drugs that can be injected into the eye to treat these blood vessels and reduce the swelling; and drugs with longer lasting effects have recently become available, such as Vabysmo and Beovue. In June 2023, Katie Curran from Queen's University Belfast in the UK and colleagues, updated their Cochrane review of these drugs and used a network meta-analysis to compare their effects. Here's Katie to tell us more.

Diabetic macular oedema is a common complication of diabetes, in which damage to the blood vessels at the back of the eye leads to swelling. Lucentis, Eylea and Avastin are three antiangiogenic drugs that can be injected into the eye to treat these blood vessels and reduce the swelling; and drugs with longer lasting effects have recently become available, such as Vabysmo and Beovue. In June 2023, Katie Curran from Queen's University Belfast in the UK and colleagues, updated their Cochrane review of these drugs and used a network meta-analysis to compare their effects. Here's Katie to tell us more.

References Steroids. 2022 Sep;185:109053 Trends Neurosci. 2015 Jun; 38(6): 364–374 Progress in Molecular Biology and Translational Science Volume 168.Pages 257-275 Histol Histopathol. 2018 Jan; 33(1): 1–10. --- Send in a voice message: https://podcasters.spotify.com/pod/show/dr-daniel-j-guerra/message Support this podcast: https://podcasters.spotify.com/pod/show/dr-daniel-j-guerra/support

In today's episode with Dr. Tom Guilliams, you'll hear us take a deep dive on: 1. The secrets of the endothelial glycocalyx 2. Potential biomarkers for assessing glycocalyx activity 3. The role of glycocalyx in cardiovascular health 4. Lifestyle factors that support endothelial health 5. Insights for maintaining cardiovascular health Dr. Tom Guilliams serves as the Director at Point Institute and the Adjunct Associate Professor at University of Wisconsin School of Pharmacy, boasting two decades of experience in the critical assessment of lifestyle, nutritional, and dietary supplement strategies aimed at both preventing and managing chronic diseases. Order tests through Rupa Health, the BEST place to order functional medicine lab tests from 30+ labs - https://www.rupahealth.com/reference-guide

Come for the yoga metaphors, stay for the science. In this episode, Associate Editor Dr. Keith Brunt (Dalhousie University) interviews author Dr. Stacy Hunter (Texas State University) and expert Dr. Annet Kirabo (Vanderbilt University) about the new study by Hunter et al., which examined the impact of hot yoga on sodium-induced pressor responses and endothelial function of Black women. In a randomized control trial that combined the thermal stress of hot yoga and a salt challenge, the authors investigated human homeostatic regulation in terms of whole-body physiology, cardiorenal responses, physical activity, and the exercise environment. In their study, Hunter and collaborators controlled for sodium intake by separating participants into high and low sodium groups, with pre and post analyses of body mass, ambulatory blood pressure, urinalysis, and flow mediated dilation. The authors found that participants who actively engaged in hot yoga showed increased flow mediated dilation but not increased blood pressure. Why did the authors use a 3-day, not 5-day, hot yoga exercise protocol? What insights can be gained about salt-sensitivity in Black women from this study, which incorporated a form of exercise that activated both thermoregulation and the parasympathetic nervous system? Listen to find out. Stacy D. Hunter, Stavros A. Kavouras, and Mitra Rahimi Exploring heated exercise as a means of preventing the deleterious effects of high-sodium intake in Black women Am J Physiol Heart Circ Physiol, published May 4, 2023. DOI: 10.1152/ajpheart.00699.2022

Dr. Matt Feng interviews Drs. Koji Kitazawa and Shigeru Kinoshita about their research on endothelial cell loss after corneal transplantation from their Ophthalmology Science article, “The Biologic Character of Donor Corneal Endothelial Cells Influences Endothelial Cell Density Post Successful Corneal Transplantation.” The Biologic Character of Donor Corneal Endothelial Cells Influences Endothelial Cell Density Post Successful Corneal Transplantation Kitazawa, Koji et al. Ophthalmology Science, Volume 3, Issue 2, 100239

Growing endothelial cells in microgravity is an accurate way to test the effects and toxicity of new vessel-targeting drugs.

A new research perspective was published in Oncotarget's Volume 14 on May 10, 2023, entitled, “HER3- A key survival pathway and an emerging therapeutic target in metastatic colorectal cancer and pancreatic ductal adenocarcinoma.” Colorectal cancer (CRC) and pancreatic ductal adenocarcinoma (PDAC) are highly metastatic cancers with poor survival rates. The tumor microenvironment has been shown to play a critical role in cancer progression and response to therapies. Endothelial cells (ECs) are a key component of the tumor microenvironment and promote cancer cell survival by secreting soluble factors that activate cancer-promoting signaling pathways. In this new perspective, researchers Omkar Desai and Rui Wang from Case Western Reserve University and University Hospitals Cleveland Medical Center discuss their studies and others that have identified HER3 as a key mediator of liver EC-induced chemoresistance and cancer cell growth in metastatic CRC and PDAC. “In complement to our studies, prior preclinical studies have shown that HER3-targeted therapies with antibodies and inhibitors have been effective in blocking tumor growth in several types of cancers [22, 23], specifically breast cancer [24], head and neck squamous cell carcinoma (HNSCC) [25], PDAC [25], and non-small cell lung cancer (NSCLC) [26]. However, translating the preclinical findings to clinical studies has shown limited impact on patient outcomes.” In this article, the researchers discuss that HER3-targeted therapies may be effective in treating patients with HER3-expressing CRC and PDAC, and highlight the importance of applying HER3 expression as a predictive biomarker for patient response to HER3-targeted therapies. They also discuss the challenges encountered in past clinical trials of HER3-targeted therapies, including the role of NRG1 gene fusions, alternative HER3 activation mechanisms, and adaptive resistance mechanisms. Finally, the team concludes by suggesting the future directions of HER3-targeted therapies, including novel approaches to overcome chemoresistance and promote cancer cell death. “In summary, we discovered that the surrounding liver EC microenvironment plays a key role in activating HER3 and promoting cell survival in mCRC and mPDAC, and potentially other types of cancer that metastasize to the liver.” DOI - https://doi.org/10.18632/oncotarget.28421 Correspondence to - Rui Wang - rxw517@case.edu Sign up for free Altmetric alerts about this article - https://oncotarget.altmetric.com/details/email_updates?id=10.18632%2Foncotarget.28421 Subscribe for free publication alerts from Oncotarget - https://www.oncotarget.com/subscribe/ Keywords - HER3, colorectal, pancreatic cancer, metastasis, microenvironment About Oncotarget Oncotarget is a primarily oncology-focused, peer-reviewed, open access journal. Papers are published continuously within yearly volumes in their final and complete form, and then quickly released to Pubmed. On September 15, 2022, Oncotarget was accepted again for indexing by MEDLINE. Oncotarget is now indexed by Medline/PubMed and PMC/PubMed. To learn more about Oncotarget, please visit https://www.oncotarget.com and connect with us: SoundCloud - https://soundcloud.com/oncotarget Facebook - https://www.facebook.com/Oncotarget/ Twitter - https://twitter.com/oncotarget Instagram - https://www.instagram.com/oncotargetjrnl/ YouTube - https://www.youtube.com/@OncotargetJournal LinkedIn - https://www.linkedin.com/company/oncotarget Pinterest - https://www.pinterest.com/oncotarget/ Reddit - https://www.reddit.com/user/Oncotarget/ Media Contact MEDIA@IMPACTJOURNALS.COM 18009220957

You know it's coming — that familiar pre-workout tingle. It creeps up your neck, sweeps across your face, and dances down your arms. Some people love it, and some don't. But have you ever stopped to wonder what causes that curious sensation? Is it a rash? A negative side effect? Do you have an allergy to pre-workout supplements? Or is there something beneficial about that pre-workout tingle or itch you feel a few minutes after drinking it? In this article, I'll explain what makes you feel flushed and itchy or what gives you the tingles. However, I won't get into whether you should regularly use pre-workout or energy drinks. I did cover whether teens should use pre-workout supplements in the past, but I'll get into the pros and cons of pre-workout supplements at another time. Vasodilation and Nitric Oxide: The Cause of the Pre-Workout Tingle Vasodilation, dilation of your blood vessels, causes the pre-workout tingle. As your blood vessels widen, they allow more blood, oxygen, and nutrients to reach your muscles. The relaxation of blood vessels is a normal and healthy response. You dilate and constrict your blood vessels to regulate blood flow, ensuring that every part of your body, from your brain to your muscles, gets the oxygen and nutrients it needs. Vasodilation plays a significant role during physical activity. When you exercise, your muscles need more oxygen to produce energy. At the heart of this process is a molecule called nitric oxide. Nitric oxide signals the smooth muscle in your blood vessels to relax, causing them to expand.Förstermann, U., & Sessa, W. C. (2012). Nitric oxide synthases: regulation and function. European heart journal, 33(7), 829-837. Nitric oxide also signals the blood vessels in your muscles to expand, increasing blood flow and allowing more oxygen to reach your working muscles. Various factors can reduce nitric oxide production, including aging, poor diet, lack of physical activity, and certain health conditions like diabetes and heart disease. When nitric oxide levels decrease, it can lead to a condition known as endothelial dysfunction.Toda, N., & Okamura, T. (2013). The pharmacology of nitric oxide in the peripheral nervous system of blood vessels. Pharmacological reviews, 55(2), 271-324. The endothelium is the inner lining of your blood vessels. It plays a critical role in maintaining vascular health. Endothelial dysfunction leads to vasoconstriction — the narrowing of blood vessels. This reduces blood flow, leading to high blood pressure and other cardiovascular issues.Lundberg, J. O., Weitzberg, E., & Gladwin, M. T. (2008). The nitrate–nitrite–nitric oxide pathway in physiology and therapeutics. Nature Reviews Drug Discovery, 7(2), 156-167. So, even though vasodilators have become extremely popular in pre-workout supplements, they also play an essential role in maintaining cardiovascular health for those who've developed heart disease. Compounds That Trigger the Pre-Workout Tingle Today, beta-alanine is the most popular vasodilator for pre-workout supplements, but other vasodilators exist. I'll briefly explain each. Beta-Alanine Beta-alanine is a non-essential amino acid that, when ingested, combines with histidine to form carnosine. Carnosine acts as a buffer, delaying the onset of muscle fatigue and enhancing your performance. However, when beta-alanine binds to nerve receptors, it can cause a sensation known as paresthesia (par·es·the·si·a) — the infamous pre-workout tingle or a feeling like you're getting poked with pins and needles. A systematic review published in the journal Amino Acids examined 15 studies on beta-alanine. The authors found that beta-alanine supplementation improved performance in high-intensity exercises lasting 1-4 minutes by 2.85%.Hobson, R. M., Saunders, B., Ball, G., Harris, R. C., & Sale, C. (2012). Effects of β-alanine supplementation on exercise performance: a meta-analysis. Amino acids, 43(1), 25-37.

This episode is brought to you by Birch Mattress by Helix, Cozy Earth, and Lifeforce. In today's minisode, Dhru discusses the lessons he's learned on his heart health journey and the critical role our endothelial health plays in our risk for heart disease. He shares what damages the endothelial lining, which coats the inside of over 60,000 miles of blood vessels, and how you can strengthen it by supporting your mitochondria, circadian rhythm, sleep, and more. Dhru also discusses his personal experience navigating high cholesterol despite making diet and lifestyle changes and how working with Dr. Michael Twyman and getting a series of lab tests helped him get a deeper look into what's going on with his heart health.In this episode, Dhru dives into:-Crazy heart disease statistics (00:42)-Endothelial dysfunction as the root cause of heart disease (4:30)-The three events that lead up to a heart attack (6:28)-How plaque forms in our arteries (11:30)-Important lab tests for cardiovascular health(12:37)-Dhru's heart health history (13:40)-Simple strategies to protect your endothelial lining (18:53)-Supporting and protecting your mitochondria (19:57)-Tips for programming your circadian rhythm (21:06)-Sleep hygiene and heart health (22:09)-Stress management and heart health (22:36)-Dietary factors (24:37)-Smoking and vaping (27:15)Also mentioned in this episode:-Sign up for the Try This Newsletter here.-Try This: Becoming Heart Attack-Proof by Protecting Your Endothelial Health-Try This: The Miracle Molecule You Need To “NO” About-Episode 158 with Dr. Louis Ignarro-Episode 383 with Dr. Michael Twyman-Dr. Michael Twyman's website, drtwyman.com. -Dr.Michael Twyman's @drtwyman-Episode 378 with Dr. Sunjya Schweig Sleep Master ClassBirch mattress by Helix is made of organic latex, cotton, New Zealand wool, and American steel springs. To get 20% off your Birch Living mattress, head to birchliving.com/dhru today.Cozy Earth has the most comfortable, temperature-regulating, and non-toxic sheets on the market. Right now, get 40% off your Cozy Earth sheets. Just head over to cozyearth.com and use code DHRULifeforce is a revolutionary new health platform that's changing the game when it comes to personalized performance and longevity-based action plans. Right now, get 15% off your first purchase at Mylifeforce.com with code DHRU. Hosted on Acast. See acast.com/privacy for more information.

This episode is brought to you by InsideTracker, WHOOP, and Levels.Today on The Dhru Purohit Podcast, Dhru sits down with his cardiologist, Dr. Michael Twyman, to discuss the many nuances around cardiovascular health, the top underrated risk factors contributing to heart disease, and how to protect your endothelial lining using diet, exercise, sunlight, sleep, and stress management. Dr. Michael Twyman is a board-certified cardiologist focusing on the prevention and early detection of heart disease. Dr. Twyman completed his cardiovascular training at St. Louis University after he completed a four-year active-duty tour as an internist at Naval Hospital Beaufort. He has been in private practice since 2012 and uses the best of conventional, quantum, and Functional Medicine and biohacking to get to the root cause of his patients' cardiovascular issues.In this episode, Dhru and Dr. Michael Twyman dive into:-Top myths in cardiology (3:44)-The limitations of cardiometabolic markers for assessing heart-disease risk (7:22)-Endothelial dysfunction as a risk factor for heart disease (16:49)-LDL cholesterol: is it good or bad? (25:10)-The top drivers damaging endothelial cells (28:30)-Light exposure: an underappreciated area of health (32:58)-How light impacts mitochondrial and heart health (37:45)-The importance of sleep quality for heart health (53:00)-The importance of stress management for health (57:28)-The nuances of cholesterol, lipoproteins, and protecting the endothelial lining (1:04:28)-Dhru's cardiovascular lab test results (1:07:05)-Looking at lipids from a plant-based vs. personalized perspective (1:39:51)-Thoughts on personalized medicine, drugs, and treatment (1:43:20)-Dr. Twyman's thoughts about seed oils and inflammation (1:50:58)Also mentioned in this episode:-Sleep Master Class with Dr. Sunjya Schweig (Episode 378)-Boston Heart Diagnostics-Cleerly ScanFor more on Dr. Michael Twyman, follow him on Instagram @drtwyman, or through his website, drtwyman.com. InsideTracker provides detailed nutrition and lifestyle guidance based on your individual needs. Right now, they're offering my podcast community 20% off. Just go to insidetracker.com/DHRU to get your discount and try it out for yourself.WHOOP is a personalized digital fitness tracker and health coaching platform that monitors your physiology 24/7 and provides personalized recommendations based on what your body needs. To get yours, go to join.whoop.com and to 15% off your membership with code DHRU15.By leveraging biosensors like continuous glucose monitors (CGM), Levels provides real-time feedback on how diet and lifestyle choices impact your metabolic health. Right now, they are offering my listeners two free months of their Levels Membership. Head on over to levels.link/DHRU to learn more. Hosted on Acast. See acast.com/privacy for more information.

Episode #251. Cardiovascular disease is one of the biggest killers in the Western world, and it's vital that we understand cardiovascular health in order to improve lifespan and healthspan. In this episode, I sit down with Dr Thomas Dayspring for Part One of a three-part deep dive into cardiovascular disease and blood lipids. In this introductory episode, we examine how our body absorbs lipids and transports them throughout the body – and what happens when it goes wrong. Specifically, we discuss: Intro (00:00) Turning a family heartache into a mission (05:12) Atherosclerosis and the role of lipids (14:52) Lipoproteins & cholesterol transport and recycling (28:01) Cholesterol's role in lipoproteins (54:00) Is ApoB a more accurate marker of cardiovascular risk? (58:38) Endothelial transcytosis of lipoproteins (1:07:10) LDL aggregation (1:16:42) LDL particle retention and inflammation (1:23:15) Difference between veins and arteries (1:28:55) Atherosclerosis and genetics (1:30:22) High-fat diets and cholesterol (1:44:01) Fiber and cholesterol absorption (1:48:14) Outro (1:57:48) The best place to connect with Dr Thomas Dayspring is on Twitter, @DrLipid. Learn more about Dr Dayspring's career and current work on LinkedIn. Discover more insights and supporting studies in the full show notes. To assist with the digestibility of this content my team and I, along with Dr Dayspring, have added a bunch of illustrations and graphs to the video format of these episodes. So if you are visually inclined you can watch these episodes on our YouTube channel. Even if you prefer listening to the first time through audio, I highly recommend coming back for a second pass with the video versions.  We've also got a Summary PDF being created with the key learnings from each part of the series. To receive a copy of this simply submit your email at theproof.com/lipidseries.  And we are working on transcripts for these episodes too (release date TBC).  Enjoy, friends. Simon Want to support the show? The best way to support the show is to use the products and services offered by our sponsors. To check them out, and enjoy great savings, visit theproof.com/friends. You can also show your support by leaving a review on the Apple Podcast app and/or sharing your favourite episodes with your friends and family. Simon Hill, MSc, BSc (Hons) Creator of theproof.com and host of The Proof with Simon Hill Author of The Proof is in the Plants Watch the episodes on YouTube or listen on Apple/Spotify Connect with me on Instagram, Twitter, and Facebook Nourish your gut with my Plant-Based Ferments Guide Download my complimentary two-week meal plan and high protein Plant Performance recipe book

In today's era, where most men, especially the youth, only know how to eliminate fat, where they should be building muscle and focusing on their testosterone levels, there is a lot of misinformation spread around. In today's episode, Ali, the queen of men's health, shares her amazing insights about the health benefits of building muscles and how to optimize their testosterone. Also, she will give us true information about cholesterol and TRT. Ali helps busy men get jacked, look great naked, optimize testosterone, and conquer nutrition by providing structure and a plan that works for their lifestyle. She has coached thousands of attorneys, doctors, other coaches, CEOs, executives, veterans, current military, and any dude looking to optimize their health.   Key Highlights: [00:01 - 09:49] Opening Segment ●        Ali introduces herself and explains why she was named the queen of men's health. ●        She also explains that statins have not done much to lower cholesterol, and more insulin levels and inflammation contribute to heart disease. ●        She also shares that there is no such thing as good or bad cholesterol. It's just cholesterol. [11:39 - 18:01] What Causes Erectile Dysfunction? ●        Erectile dysfunction results from three Bs, brain, biochemistry, and blood flow. ●        Endothelial dysfunction equals erectile dysfunction, as per Dr. Michael Twyman. ●        Stress affects the brain and signals that go down to the testicles and penis to procreate. ●        Having unhealthy arteries, capillaries, and other things that contribute to healthy blood flow will also have an adverse effect on your penis.  [18:02- 25:09] TRT And Self-Medication ●        Men are often stigmatized when discussing testosterone replacement therapy (TRT), leading to dismissal and self-medication. ●        Ali advises that don't go to a GP for hormone replacement; go to someone with experience and a network of people. ●        In Ali's opinion, insurance-based treatment presents a nightmare due to the arbitrary ranges that are deemed normal. ●        Peptides are amino acids that can be used for fat loss, recovery, and other things and can be bought online as research chemicals.   Key Quotes: “The rate of muscle decline accelerates as we get older.”  Ali Gilbert   “Everyone is going to react different, they have different needs and stuff like that. So sending somebody out the door with testosterone and other things that are not necessary, it's just malpractice, in my opinion.” - Ali Gilbert   “If somebody says, I wanna get jacked, what does that mean to you? Because to me, it's a whole different definition.” - Ali Gilbert   “The more body fat you have, the more inflamed you are because body fat is the most inflammatory tissue on the body.” - Ali Gilbert     CONNECT WITH  ALI : Ali Gilbert website Instagram.com/thealigilbert The Silverback Summit   Get leaner. Live Longer. Be Legendary. Here's how I can help you reach your goals! 1. Visit N8training.com - mastermind 2. Join our 5-Day Morning Routine Challenge 3. Get my super easy and accessible FREE 5-Day Sugar Detox Program. All you have to do is put in your email and receive access together with a handbook! Thefreesugardetox.com 4.  Start by understanding the science and simplicity of carb backloading for fat loss - go to GetNatesBook.Com. to get a free copy of Nate's bestseller “The Million Dollar Body Method” 5. Get more great tips to get leaner by connecting with me on Instagram @lowcarbhustle 6. Join the MDB Mastermind for just a buck! If you want accountability, coaching, and an amazing training program to get leaner, this is what you need. Go to nate.fit to find out more and get your first 2 weeks for just 1 dollar. If you liked the show, please LEAVE A 5-STAR REVIEW, and share it on social media to get reposted to over 12k of the homies.

Did you know that some ED medications can have serious side effects that go far beyond the bedroom? From sudden vision loss to an increased risk of heart attack, these drugs can have a significant impact on your health.If you're curious to learn more about the potential risks associated with ED medication, tune in - the answers may surprise you.CHAPTERS0:00 - INTRO1:18 - How do you get an erection?3:21 - Endothelial dysfunction and blood flow.5:07 - Medications that are most likely to cause a DDS.6:29 - Dr. Anne Truong's free gift is going to give you more sex.8:37 - How to test your nitrous oxide level in your saliva.10:10 - What are the side effects of EDI medication?12:24 - What's the effectiveness of these medications like?14:39 - Medication Interaction17:06 - Why taking medication does not cure your disease20:56 - OUTROIf you like this episode, please SUBSCRIBE, like, leave a comment, and share so we can keep bringing you valuable content that gets results!--------------Follow Me On:InstagramTwitterFacebookTikokYouTube--------------Want to regain control of your sex life? It's time to reverse the effects of ED on your life. Join the Modern Man Club and start your road to full recovery and community.https://mensexualityclub.com/salespage--------------For all links and resources mentioned on the show and where to subscribe to the podcast, please visit https://sexualhealthformenpodcast.com/ed-medications-side-effects/--------------Reveal the FREE treatment most men ignore that solves thousands of erectile dysfunction cases every year, plus the 5 biggest mistakes you must avoid if you want to say goodbye to your ED. Uncover it all in my free ebook, available to download now.https://ed.truongrehab.com/ebook?utm_source=podcastandutm_campaign=eBook

Nathan S. Bryan, PhD is an academic and entrepreneur who has focused his career on the study of nitric oxide for nearly 25 years. He joins the podcast to contribute to the Evolution of Medicine's current focus on cardiovascular and endothelial health.   Nitric oxide production is foundational to overall health. Unfortunately, many contemporary environmental and lifestyle factors reduce or prevent nitric oxide production in the body. Suboptimal nitric oxide production is a factor in insulin resistance, cardiovascular issues, erectile dysfunction, long COVID and other post-viral syndromes, chronic fatigue, cognitive disorders, and more. Tune into this episode to learn about the many lifestyle and environmental factors that can impede or support the production of this important molecule. Some may even surprise you! Listen to the episode to learn more about: Nitric oxide's role in cardiovascular, immune and nervous system function The gold standards for measuring functional nitric oxide production James' and Dr. Bryan's views on the public health response during the COVID era Using nitric oxide to heal inflammation caused by excess spike protein The risks of using some commercially available products that claim to boost nitric oxide Also, check out Dr. Bryan's educational website, which includes a blog, videos and more: https://drnathansbryan.com/

#6 What happens when your heart doesn't perform as it should, and the answer isn't clear? In this episode, Rob Thornett reveals his fight against an elusive heart condition, his eventual lifesaving surgery, and the aftermath. He and Boots Knighton discuss the steep learning curve with medical terminologies, the power of patient advocacy, and the unspoken toll of being misdiagnosed. Rob also uncovers fascinating insights about the genetic links of myocardial bridge conditions and the remarkable role of Facebook support groups. Experience their stories of recovery, resilience, and the community's role in healing.Website: The Heart Chamber (theheartchamberpodcast.com)Transcript: Joyful Beat | The Heart Chamber (theheartchamberpodcast.com)The Heart Chamber (@theheartchamberpodcast)Thanks to Michael Moeri for being my right hand man. Michael Moeri - Audio Editor, Podcast Producer and Marketing DirectorThanks to Denise Hardy for your support and believing in me. linkedin.com/in/denise-hardy-30a51086Music by AudioCoffee0:00 intro1:30 Boots explains her reason for bringing Rob onto the Podcast. 2:10 Rob summarizes Boots's story and how they came to know each other.2:50 Rob tells us about his teaching career and how he always had a nagging "issue" and how many of us Heart Warriors are told we have anxiety4:20 Rob dives right into the myths of myocardial bridges6:00 Then, while in China, he began to really struggle7:40 He rattles through the various ER visits 8:10 He begs for a heart CT and offers to pay in cash at a rural Chinese hospital9:15 Rob notices there is "note" on the Chinese CT report and pushes for translation11:10 Rob finds himself infront of a Chinese hospital wondering what to do now with the knowledge that he had a myocardial bridge12:45 No one found Rob's myocardial bridge in the United States and often patients in the US are not told that myocardial bridges are found14:00 Boots emphasizs the importance of reading your own doctor's reports after doctor visits15:00 Rob gets to work trying to find someone in the US to help him16:00 Stanford is the main team in the US for myocardial bridging17:30 Rob walks us through how he got insurance back in the States 18:00 Boots shares which arteries were bridged on her heart20:30 Rob explains the holding pattern he entered as he waited21:30 Rob begins to deteriorate even as he taught school23:00 As Rob waits for Stanford, he unsuccessfully pursues robotic surgery28:00 Side story: Rob shares stories of other patients experiencing the issues with getting diagnosed properly28:00 Rob finally had surgery at Stanford and walks us through the process32:10 Boots shares her surgery process during Covid40:00 Rob goes into the Facebook support group for myocardial bridging42:30 Boots reflects on getting to wake up every day44:00 Rob goes back to work after surgery 17 days after surgery!47:00 Doctors who are doing robotic surgery now with success that have been shared on the Facebook site49:00 Do not let a doctor do a bypass on your bridge!52:00 Six months post op until present day 52:30 Endothelial dysfunction explained and how it takes time to heal55:00 Boots reflects on walking the day after surgery as well as her recovery57:00 Boots has been in a...

Until five months ago, I was getting gout attacks once or twice a month. My doctor had upped my medication and added another medication as well. No help! But since I started taking a nitric oxide supplement (Cardio Miracle) five months ago, I have only had three gout attacks and have dropped one of the medications. This is on top of the other significant changes in my labs and hard measurements of health. In this episode, we talk about endothelial cells, their function in the body, and their role in inflammation and sickness. And of course, we talk about the hard, scientific studies on nitric oxide in the body. More on Cardio Miracle- https://cwic.qwkcheckout.com Website- www.cwicmedia.com 

Is inflammation the driving force for diastolic dysfunction in heart failure with preserved ejection fraction (HFpEF)? In this episode, Deputy Editor Dr. Zamaneh Kassiri (University of Alberta) interviews author Dr. Thassio Mesquita (Cedars-Sinai Medical Center) and expert Dr. Darryl Davis (University of Ottawa Heart Institute) about the research by de Couto et al. Using a Dahl salt-sensitive rat model of HFpEF, the authors delivered cardiosphere-derived cells (CDCs) via intracoronary injection into the microcirculation. After 2 weeks of treatment with CDCs, the hypertensive rats showed improved endothelial-dependent vasodilation, reduced oxidative stress, restored expression of endothelial nitric oxide synthase, and reduced inflammation. Overall, the authors found that CDCs made significant improvements in the cardiovascular health of hypertensive rats with HFpEF. What is the therapeutic potential of cardiosphere-derived cells for treating heart failure with preserved ejection fraction (HFpEF)? Listen and learn more.   Geoffrey de Couto, Thassio Mesquita, Xiaokang Wu, Alex Rajewski, Feng Huang, Akbarshakh Akhmerov, Na Na, Di Wu, Yizhou Wang, Liang Li, My Tran, Peter Kilfoil, Eugenio Cingolani, and Eduardo Marbán Cell therapy attenuates endothelial dysfunction in hypertensive rats with heart failure and preserved ejection fraction Am J Physiol Heart Circ Physiol, published October 17, 2022. DOI: 10.1152/ajpheart.00287.2022

This month on Episode 44 of Discover CircRes, host Cynthia St. Hilaire highlights four original research articles featured in the January 6th and January 20th issue of Circulation Research. This episode also features an interview with Dr Timothy McKinsey and Dr Marcello Rubino about their study, Inhibition of Eicosanoid Degradation Mitigates Fibrosis of the Heart.   Article highlights:   Prasad, et al. ACE2 in Gut Integrity and Diabetic Retinopathy   Cui, et al. Epsins Regulate Lipid Metabolism and Transport   Li, et al. Endothelial H2S modulates EndoMT in HF   Luo, et al. F. plautii Attenuates Arterial Stiffness   Cindy St. Hilaire:        Hi, and welcome to Discover CircRes, the podcast of the American Heart Association's Journal Circulation Research. I'm your host, Dr Cindy St. Hilaire from the Vascular Medicine Institute at the University of Pittsburgh. And today I'm going to be highlighting articles from our January 6th and January 20th issues of Circulation Research. I'm also going to have a chat with Dr Timothy McKinsey and Dr Marcello Rubino about their study, Inhibition of Eicosanoid Degradation Mitigates Fibrosis of the Heart. But before the interview, I want to get to a few articles to highlight.   Cindy St. Hilaire:        The first article is titled, Maintenance of Enteral ACE2 Prevents Diabetic Retinopathy in Type 1 Diabetes. The first authors are Ram Prasad and Jason Floyd, and the corresponding author is Maria Grant, and they are from the University of Alabama.   Type 1 Diabetes has a complex etiology and pathology that are not entirely understood. In addition to the destruction of insulin-producing cells, a recently discovered feature of the disease in both humans and in rodent models is that the levels of angiotensin converting enzyme 2 or ACE2 can be unusually low in certain tissues. ACE2 is a component of the renin angiotensin system controlling hemodynamics and interestingly, genetic deficiency of ACE2 in rodents exacerbates aspects of diabetes such as gut permeability, systemic inflammation and diabetic retinopathy, while boosting ACE2 has been shown to ameliorate diabetic retinopathy in mice. This study shows that ACE2 treatment also improves gut integrity and systemic inflammation as well as retinopathy. Six months after the onset of diabetes in a mouse model, oral doses of a bacteria engineered to express humanized ACE2 led to a reversal of the animal's gut barrier dysfunction and its retinopathy. Humans with diabetic retinopathy also displayed evidence of increased gut permeability in low levels of ACE2. This study suggests they may benefit from a similar probiotic treatment.   Cindy St. Hilaire:        The next article I want to highlight is titled, Epsin Nanotherapy Regulates Cholesterol Transport to Fortify Atheroma Regression. The first authors are Kui Cui, Xinlei Gao and Beibei Wang, and the corresponding authors are Hong Chen and Kaifu Chen and they're from Boston Children's Hospital. Epsins are a family of plasma membrane proteins that drive endocytosis. They're expressed at varying levels throughout the tissues of the body, and recent research shows that they are unusually abundant on macrophages within atherosclerotic lesions. In mice, macrophage specific Epsin loss results in a reduction in foam cell formation and atherosclerotic plaque development. This study now shows that this effect on foam cells is because Epsins normally promote the internalization of lipids into macrophages through their endosytic activity.   But that's not all. The proteins also impede cholesterol efflux from macrophages to further exacerbate lipid retention. It turns out out Epsins regulate the endocytosis and the degradation of a cholesterol efflux factor called ABCG1. Importantly, these pro atrogenic activities of Epsins can be stopped. Using macrophage targeted nanoparticles carrying Epson specific silencing RNA, the team could suppress reduction of the protein in cultured macrophages and could reduce the size and number of plaques in atherosclerosis prone mice. Together these results suggest blocking Epsins via nanotherapy or other means could be a therapeutic approach to stopping or slowing atherosclerotic plaque progression.   Cindy St. Hilaire:        The third article I want to highlight is coming from our January 20th issue of Circ Res and is titled, Hydrogen Sulfide Modulates Endothelial-Mesenchymal Transition in Heart Failure. The first author is Zhen Li, and the corresponding author is David Lefer and they're from Cedars-Sinai. Hydrogen sulfide is a critical endogenous signaling molecule that exerts protective effects in the setting of heart failure. Cystathionine γ-lyase, or CSE, is one of the three hydrogen sulfide producing enzymes, and it's predominantly localized in the vascular endothelium. Genetic deletion of CSE, specifically in the endothelium, leads to reduced nitric oxide bioavailability, impaired vascular relaxation and impaired exercise capacity, while genetic over-expression of PSE in endothelial cells improves endothelial cell dysfunction, and attenuates myocardial infarction following myocardial ischemia-reperfusion injury.   In this study, endothelial cell specific CSE knockout mice and endothelial cell specific CSE overexpressing transgenic mice were subjected to transverse aortic constriction to induce heart failure with reduced ejection fraction. And the goal was to investigate the contribution of the CSE hydrogen sulfide access in heart failure. Endothelial specific CSE knockout mice exhibited increased endothelial to mesenchymal transition and reduced nitric oxide bioavailability in the myocardium. And this was associated with increased cardiac fibrosis, impaired cardiac and vascular function, and it worsened the vascular performance of these animals. In contrast, genetic overexpression of CSE in endothelial cells led to increased myocardial nitric oxide, decreased EndoMT and decreased cardiac fibrosis. It also improved exercise capacity. These data demonstrate that endothelial CSE modulates endothelial mesenchymal transition and ameliorated the severity of pressure overload induced heart failure , in part through nitric oxide related mechanisms. This data further suggests that endothelium derived hydrogen sulfide is a potential therapeutic for the treatment of heart failure with reduced ejection fraction.   Cindy St. Hilaire         The last article I want to highlight is titled, Flavonifractor plautii Protects Against Elevated Arterial Stiffness. The first authors are Shiyun Luo and Yawen Zhao, and the corresponding author is Min Xia, and they are at Sun Yat-sen University. Dysbiosis of gut microbiota contributes to vascular dysfunction and gut microbial diversity has been reported to be inversely correlated with arterial stiffness. However, the causal role of gut microbiota in the progression of arterial stiffness and the specific species along with the molecular mechanisms underlying this change remain largely unknown. In this study, the microbial composition in metabolic capacities were compared in participants with elevated arterial stiffness and in normal controls free of medication. And these groups were age and sex match.   Human fecal metagenomic sequencing identified a significant presence of Flavonifractor plautii or F. plautii in normal controls, which was absent in the subjects with elevated arterial stiffness. The microbiome of normal controls exhibited an enhanced capacity for glycolysis and polysaccharide degradation, whereas individuals with increased arterial stiffness exhibited increased biosynthesis of fatty acids and aromatic amino acids. Additionally, experiments in the angiotensin II induced and humanized mouse model show that replenishment with F. plautii or its main effector cis-aconitic acid or CCA improved elastic fiber network and reversed increased pulse wave velocity through the suppression of matrix metalloproteinase-2 and through the inhibition of monocyte chemoattractant protein-1. And this was seen in both the angiotensin II induced and humanized models of arterial stiffness. This study now identifies a novel link between F. plautii and arterial function and raises the possibility of sustaining vascular health by targeting the gut microbiota.   Cindy St. Hilaire:        Today with me I have Dr Tim McKinsey and Dr Marcello Rubino from the University of Colorado Anschutz Medical Campus, and we're here to talk about their paper Inhibition of Eicosanoid Degradati`on Mitigates Fibrosis of the Heart. And this article is in our January 6th issue of Circulation Research, so thank you both so much for joining me today.   Timothy McKinsey:    Thank you for inviting us.   Marcello Rubino:        Yeah, thank you for the opportunity.   Cindy St. Hilaire:        And so Dr McKinsey, you're a professor at the University of Colorado. How long have you been investigating cardiac fibrosis?   Timothy McKinsey:    Oh, a long time. Before I started the lab here in 2010, I was in industry working in biotech with Myogenic Gilead, and we were very interested in cardiac fibrosis all the way back then.   Cindy St. Hilaire:        Oh wow, so you actually made an industry to academia transfer.   Timothy McKinsey:    Yes.   Cindy St. Hilaire:        Good topic for another podcast. That is really great.   Timothy McKinsey:    Yeah, it's of interest to a lot of people, including trainees.   Cindy St. Hilaire:        Yeah, I bet. Dr Rubino, you were or are a postdoc in the McKinsey lab? Marcello Rubino:        Yeah, I was a postdoc in Timothy McKinsey lab. I spent four years in Tim's lab. It was my first time studying cardio fibrosis, so it was a little bit difficult at the end, but I think I was right choosing Tim, so I'm really happy now.   Cindy St. Hilaire:        Nice and are you sticking with fibrosis or are you moving on?   Marcello Rubino:        Yeah, so now I'm back in Milan where I did my PhD student and postdoc. I am like an independent researcher, but it's still not a principal investigator, so I want to become one of the that, studying cardiac fibrosis. Yeah. And inflammation and epigenetics, so yeah, I'm going try to go to my way, thanks to Tim, I think that I find my own way.   Cindy St. Hilaire:        I'm sure you will. I mean, based on the great work in this study, right. Building upon that, I'm sure you'll be a success.   Timothy McKinsey:    No doubt about it.   Cindy St. Hilaire:        So your manuscript, this study, it's investigating whether eicosanoid availability can attenuate fibrosis in the heart. But before we kind of jump into this study, why is fibrosis in the heart a bad thing? Is it always detrimental? Is there some level of fibrosis that's necessary or even helpful?   Timothy McKinsey:    I mean, a certain level of extracellular matrix is deposited in your heart and that maintains the structure of the heart. Fibrosis can also be good after you have a myocardial infarction and a big piece of the muscle of your heart has died, it needs to be replaced with a fibrotic scar, essentially to prevent rupture of the ventricle. So fibrosis isn't always bad, but chronic fibrosis can be really deleterious to the heart and contribute to stiffening of the heart and cause diastolic dysfunction. It can create substrates for arrhythmias and sudden cardiac death. So we're really trying to block the maladaptive fibrosis that occurs in response to chronic stress.   Cindy St. Hilaire:        Yeah, yeah. And what about eicosanoids? What are they and what role do they play in cardiac fibrosis or what was known about their role in this process before your study?   Timothy McKinsey:    Eicosanoids are lipids, they're basically fatty acids, 20 carbon in length and a lot is known about them. It's a very complex system. There are many different eicosanoids, but they're produced from arachidonic acid through the action of cyclooxygenase enzymes like COX-2. And so you're probably familiar with the literature showing that non-steroidal anti-inflammatory drugs that target the COX enzymes can actually increase the risk of cardiac disease, so there was a lot known about what produces eicosanoids in the heart, but our study is really the first to address how they're degraded and how that controls cardiac fibrosis.   Cindy St. Hilaire:        What I thought you did really well in the introduction and what I guess I didn't really fully appreciate until I had read your study, was that your goal was to identify compounds that could attenuate fibrosis. And you spent some time emphasizing the differences between a targeted small molecule screen and a phenotype based screen. And I was wondering if you could just expand on this difference for the audience and maybe just explain why in your case you went with the latter.   Timothy McKinsey:    Well, we wanted to use an unbiased approach and some people call this a chemical biology approach where we took a targeted library, meaning we took compounds with known activities, meaning compounds that with known targets and we screened that library using a phenotypic assays that we developed in the lab. And the phenotypic assay is an unbiased assay, right? We're just screening for compounds that have the ability to block the activation of fibroblasts. And we monitor activation by looking at markers of fibroblast activation such as alpha smooth muscle Actin. And we can do this in a very quantitative and high throughput manner using this imaging system, high content imaging system that we have in the lab.   It was an unbiased screen looking for inhibitors of fibroblasts activation across organ systems. We not only studied cardiac fibroblasts, but we also studied lung and renal fibroblasts looking for compounds with a common ability to block the activation state of each of those cell types.   One of the things that I get asked frequently is how do we maintain the cardiac fibroblasts in a quiescent state? Because you may know this, but when fibroblasts are plated on cell culture plastic, which has a very high 10 cell strength, they tend to spontaneously activate, so we actually spent a couple of years working out the conditions to maintain the cells in quiescent state, and I think that will also be of great interest to the field.   Cindy St. Hilaire:        Probably even the smooth muscle cell biology field where I hang out and even valve interstitial cells that we study. All of those, I guess basic things related to cell culture, we have taken for granted that plastic is not physiological.   Timothy McKinsey:    Right.   Cindy St. Hilaire:        And so I think with this really nice phenotypic or chemical screen that you conducted, you first identified nine compounds, but what made you zero in on this one, SW033291?   Timothy McKinsey:    When we got the hits, we were intrigued by the SW compound SW033291 because there was only one paper describing its action and there was a paper published in Science showing that SW or inhibition of this enzyme 15-PGDH could enhance organ regeneration.   Cindy St. Hilaire:        Oh, okay.   Timothy McKinsey:    And there's a very interesting interplay between fibrosis and organ regeneration where fibrosis inhibits regeneration and if you can stimulate regenerative pathways, they can actually block fibrosis, so there's this back and forth. And so that's really the main reason we were interested in pursuing SW just because of the novelty and the potential. And also it was a compound that behaved beautifully in our cell culture models with beautiful dose-dependent inhibition of each of the fibroblast types.   Cindy St. Hilaire:        It's kind of like the cleanest thing to start with. Also, if there's nothing known, it's ripe for investigation, so that's great. You just said this SW compound acts on 15-PGDH, so what is the role of that protein in fibroblasts and what if any known effects are there on this protein's inhibition in other cell types or disease states?   Marcello Rubino:        In fibroblasts team, I would like to say that this was really the first article that was published. Maybe there was just one published in Pulmonary Fibrosis, but like last year, but I didn't really talk about 15-PGDH, so you need to consider that 15-PGDH is an inhibitor, an enzyme that degrades prostaglandin, so if you inhibit the inhibitor, the release increase production, a lot of prostaglandin. And so a lot of paper were talking about this effect, so they will see we are just using SW in order to increase Prostaglandin E2 level and that was why we had this like anti-inflammatory or whatever effect. I would like to say that until now, maybe this can be the first really paper talking about no more than not just prostaglandin but 15-PGDH. Its action total level, a global level at particularly on fibroblasts.   To answer your question, I would like to say that this was also our question first and we checked by level other browser to try to find the answer to your question. We figured out that it was known that 15-PGDH was increasing a pathology condition in different organ, not just related by fibroblasts, not just related to cardiac disease, about the function with discover a function in macrophage that interested us because it can regulate maybe the polarization macrophage, so still involving the prostaglandin production inflammation, so that's why also we decide to take a look because it was still novel in fibrolbasts and we still know that it was doing something important and we were trying not to put the piece together and find something new in that we were lucky for this.   Timothy McKinsey:    15-PGDH is actually expressed at very low levels in fibroblasts. It's much more highly expressed in macrophage, just as Marcello pointed out, so in the future we're very interested in knocking out or inhibiting 15-PGDH in different cell types to see how that contributes to inhibition of cardiac fibrosis.   Cindy St. Hilaire:        Really interesting. Related to that, you used a couple different animal models for fibrosis. They're all different or special in their own way. How well did these recapitulate what we observe in humans. Are there any limitations of benefits?   Timothy McKinsey:    They're always limitations to animal models. We started out with a very robust commonly used model of cardiac fibrosis, which relies on Angiotensin II infusion in mice. We like that model because it's robust and quick so we can get answers quickly. And then we transitioned into a model of diastolic dysfunction that we've been working with in a lab where we remove a kidney from a mouse and we implant something called DOCA, which is an aldosterone memetic. And so the animals develop hypertension that leads to a mild but significant diastolic dysfunction with preserved ejection fraction.   And that's a model that we like a lot. It has something that we call hidden fibrosis, so if you just do standard histochemical staining of the hearts from the DOCA unit, nephrectomy model, that diastolic dysfunction model, you really can't see robust fibrosis. It's only when you dive more deeply with more sensitive assays like mass spectrometry or atomic force microscopy that you can detect this fibrosis and stiffening of the heart, so we usually lead with a robust model of fibrosis, cardiac fibrosis, and then transition into a slightly more complex model but more physiologically relevant model or disease relevant model.   Cindy St. Hilaire:        Obviously you showed some really nice robust results with this SW compound. So in the continuum of heart failure in human, what do you think or what would you speculate would be the ideal timeframe for administration of this compound?   Timothy McKinsey:    Wouldn't want to give it immediately after someone's had a heart attack. As we discussed earlier, you need that reparative scar to form so you don't want to block that fibrotic remodeling. We believe that there's kind of smoldering fibroblast activation in the heart, even in someone who's had heart disease for many, many years. And if we can dampen that, we can either prevent further progression of heart failure or perhaps reverse it. We don't really know if we can reverse really established fibrosis in the heart yet. But I would want to try to catch fibrosis fairly early on in the disease process in someone who has chronic hypertension or obesity or a variety of different comorbidities and then start delivering an antifibrotic therapy at that point.   Marcello Rubino:        I would like to add that, so it is really tricky when we talk about clinical trials because a lot of molecules that maybe they can work hopefully in a preclinical model don't work at the end in the clinical model. That's because can be some off target also like you just asked what is really important is when you do the administration of the molecule and talk about this in SW, like things say we don't want to prevent the fibrosis because there is something like called a kneeling at the beginning, so it is the good fibrosis we like to say, but the good thing of SW compound is that is affecting in a good way the proliferation of fibroblast that is different for all the other. I would like to say all the other inhibitor that we saw so far, because I remember the first time that I presented this work, there was an expert told me that he didn't believe that all my data because the compound was inhibiting fibrosis, it was inhibiting proliferation.   And I show him, no, this is contrary, so oh okay, I like it. We need to consider this that the action seems to be not like the retire for the cell, so because the cells continue to proliferate, they can proliferate more. But the good thing and we need to investigate more is that SW action seems to increase when the cell are more fibrotic, because we show just few human fibroblasts isolating from a human patient and we saw a higher positive effect of SW compound when the cell were more fibrotic. That can be interesting. I think that it's worth to try to test in the future like in different preclinical models and maybe in patients at the end because if we really can find something like maybe SW that can be specific for the state of pathology, that will be wonderful. I don't really know if we can really do it, but we need some therapy like this, so that's why we were really excited about what we discovered for this compound.   Timothy McKinsey:    We have a lot more to learn about this pathway and about fibrosis in general.   Cindy St. Hilaire:        Yeah.   Timothy McKinsey:    It's a very exciting time to be doing science because of the amazing technologies that we have at our disposal to address detailed mechanisms of disease.   Cindy St. Hilaire:        What was the most challenging aspect of the study?   Timothy McKinsey:    This was an incredibly difficult study. I can't even stress to you how much work went into this. Spearheaded by Marcello's awesome leadership. There was huge input from a big team. Keith Cook and I worked together in industry and we were able to recruit him over here for a few years as part of our fibrosis center called the CFReT. It's an advertisement. And Keith was able to implement some of the drug discovery approaches that we used in biotech and create this imaging system that we initially employed for the screens. That was challenging. Maintaining the cells in a quiescent state was very challenging as I mentioned. That took a couple of years and then just following up on SW and trying to figure out its mechanism of action was really challenging as well because as Marcello mentioned, most people have attributed SW's effects to an increase in PGE2 levels, so PGE2 is an eicosanoid that is degraded by 15-PGDH.   And definitely when you inhibit 15-PGDH with SW, you see increased PGE2. But surprisingly we couldn't find that PGE2 was doing anything in our cell culture systems, meaning when we added it exogenously it was not blocking fibroblast activation, so then Marcello set out to identify which eicosanoid that is regulated by 15-PGDH is actually the antifibrotic eicosanoid. And that led him to something called 12(S)-HETE. That was challenging. And then just determining at the molecular level what was going on was also challenging. And that led Marcello to this kind of paradoxical discovery that it activating ERK signaling was actually blocking fibroblast activation.   Cindy St. Hilaire:        And of course ERK does everything right?   Marcello Rubino:        It does. Everything.   Timothy McKinsey:    And sort of the dogma is that ERK is promoting fibrosis in the heart, but Marcello's data suggests otherwise.   Timothy McKinsey:    And then other shout outs, Josh Travers, who's the second author of the paper provided huge input, especially after Marcello left. Josh helped get this across the finish line. We have an amazing in vivo team conducting the animal model studies. Maria Cavasin and Elizabeth Hardy. I could go on and on. There are a lot of authors and if I didn't mention one of them, it doesn't mean that they weren't key contributors. I just wanted to throw that out there. We also had great collaborators. I think another component of this paper that is of great interest to us, and initially I was against doing any of this, is that Marcello and Josh created this biobank of human cardiac fibroblasts that we obtained from explanted hearts from individuals undergoing heart transplantation.   And initially I thought it was going to be a waste of time and money for Marcello and Josh to do that, but they were persistent and they started isolating these cells. And the cells are really fascinating because even after you take them out of that failed human heart and culture them, they maintain this constituently active state, which is different than the cells we were using for screening where we kept them quiescent and then we stimulated them with TGF-β to activate them. These human cardiac fibroblasts from the failed human hearts are just on all the time.   Cindy St. Hilaire:        Wow.   Timothy McKinsey:    And SW does a really amazing job of reversing that activated state.   Cindy St. Hilaire:        Very cool and excellent resource I'm sure for future studies. So my last question is what's next? You know, you discovered a lot in this paper. What's the next thing you want to tackle?   Timothy McKinsey:    Cell type specific roles for 15-PGDH in the heart, in the control of cardiac homeostasis and disease. Basically we want to knock it out in fibroblasts. We want to knock it out in our macrophages and see what the consequences are. That's one thing. We want to really pursue the whole GPR31 12(S)-HETE pathway in the heart. That's something that has never been studied. And so GPR31 is a G protein coupled receptor that is bound by this eicosanoid called 12(S)-HETE. And that seems to be blocking fibroblast activation, so we're going to further pursue that pathway. And then we think that this paradoxical finding related to ERK signaling in the heart is also worthy of pursuit. Why is it that stimulating ERK in a cardiac fibroblast is actually blocking the activation state of that cell?   Marcello Rubino:        I'm interested in this like Tim says, but also interested in the role of the interaction of the cell because it's important to study like a specific gene inhibitor, whatever role in a specific cell, but what happened to the other cell, the interaction the other cell when you do knocking in some specific cell, so that's what I'm trying to do in general. Now I move back in Italy, like I told you, I'm like a kind of independent research and I'm studying a lot single cell sequencing right now. Try to do also try to see what happened to interaction, understand during pathology.   The idea is to study like inhibitor treatment and to see what really happened because gene expression is important, but we need to consider also of course the protein shape, the protein interaction, the cell interaction, so I try to grow in this field and see what really happened because the problem of the cell, they're just cell in vitro. They can mimic what happened, but it's not what really happened in vivo, so can we use this novel technology to improve our knowledge, that's what I want to try to do.   Cindy St. Hilaire:        Well that's great. Dr McKinsey, Dr Rubino, thank you so much for taking the time to speak with me today. Title of their article was Inhibition of Eicosanoid Degradation Mitigates Fibrosis of the Heart. It's in our January 6th issue of Circ Res. And thank you both so much for joining me today and thank you to you and all of your colleagues who worked so hard on this for this amazing study.   Timothy McKinsey:    Thank you. We really enjoyed this visit and we're grateful to have our work published in Circulation Research.   Cindy St. Hilaire:        That's it for highlights from the January 6th and 20th issues of Circulation Research. Thank you for listening. Please check out the CircRes Facebook page and follow us on Twitter and Instagram with the handle @CircRes or #DiscoverCircRes. Thank you to our guests, Dr Tim McKinsey and Dr Marcello Rubino. This podcast is produced by Ishara Rantayaka, edited by Melissa Stoner and supported by the editorial team of Circulation Research. Some of the copy text for highlighted articles is provided by Ruth Williams. I'm your host, Dr Cindy St. Hilaire, and this is Discover CircRes, your on-the-go source for the most exciting discoveries in basic cardiovascular research. This program is copyright of the American Heart Association 2022. The opinions expressed by the speakers of this podcast are their own and not necessarily those of the editors or of the American Heart Association. For more information, please visit ahajournals.org.  

Joining Brian and myself in our virtual studio we are delighted to have Dr Sara Lawrence-Mills, one of our fabulous residents in Anaesthesia and Analgesia here at the RVC. Sara has taken an interest into the endothelial glycocalyx and we thought we'd talk to her to find out why.  We hope that you enjoy.   Some references of interest: https://pubmed.ncbi.nlm.nih.gov/35654338/https://www.frontiersin.org/articles/10.3389/fvets.2021.661660/fullhttps://pubmed.ncbi.nlm.nih.gov/22290457/https://pubmed.ncbi.nlm.nih.gov/32067360/  To Cite this podcast as: Dom Barfield. RVC Clinical Podcast 134 Endothelial glycocalyx with Sara Lawrence-Mills. Published on September 30 2022 If you have any comments about this podcast, please get in touch: email dbarfield@rvc.ac.uk; tweet @dombarfield. We would greatly appreciate your time to rate us on Apple podcast, podbean or Acast and kindly write us a review.

In honor of Father's Day, we're thrilled to welcome Dr. Caldwell B. Esselstyn Jr. back on the podcast to answer YOUR questions. He answers questions related to cholesterol, exercise, sodium, fat, atrial fibrillation, the best diagnostic tests, and – of course – how to build an ENDOTHELIUM FORTRESS. The best Father's Day gift is sharing the wisdom and knowledge of this pioneer so let's get right to it. Episode Timestamps 4:50 It's Showtime! 5:55 I'm 100% plant-based, but have high cholesterol. Do I still need statins? 8:30 What is the best diagnostic test to see if my arteries are clogged? 9:52 Question about sodium consumption. The data is so confusing! 12:50 What are the best heart health tests I should take at 50? 14:15 LPa - What is it, and how can I improve it? 16:00 How do I build an endothelial fortress and lower my triglycerides? 18:15 Atrial fibrillation - Can it be reversed via diet? 21:45 Could I potentially have familial hypercholesterolemia? 26:39 Talk about the role of fats in a plant-based diet. Aren't some fats healthy? 31:30 What is your opinion on meds for clotting and atrial fibrillation? 33:30 Could I impact familial hypercholesterolemia with a whole foods plant-based diet? 34:14 Opinions on cardio exercise? Can too much be bad? 37:00 Can my HDL be too high? 44:05 How can I best treat mild atherosclerosis? Episode Resources Watch the Episode on YouTube Order Prevent and Reverse Heart Disease by Dr. Caldwell B. Esselstyn, Jr. Dr. Esselstyn's Prevent and Reverse Heart Disease Program Website PLANTSTRONG Foods - shop our official unsalted broths- and our growing assortment of other delicious products made without oil, added sugar, or excessive sodium. PLANTSTRONG Sedona Retreat - October 10th-15th, 2022 - Dr. Doug Lisle - the esteemed evolutionary psychologist and co-author of The Pleasure Trap - is attending our upcoming Sedona Retreat to give three of his paradigm-shifting lectures that help us understand all the forces working against us in our quest to live plantstrong. Once you SEE the system we live in - you can't UNSEE it. And Dr. Lisle is a master in giving us language and tools to set ourselves on a permanent path to success. And great news! Our Sedona retreat has been approved for 21.5 CME credits for physicians and physician assistants. And 21.5 Nursing Contact Hours for nurses…. And 2.2 CEUs for other healthcare professionals as part of the registration fee for our PLANTSTRONG Retreat. PLANTSTRONG Meal Planner - Save $10 off the annual membership with the code: PLANTSTRONG Visit plantstrong.com for all PLANTSTRONG Resources, including books, recipes, foods, and the PLANTSTRONG Coaching Programs Join the Free PLANTSTRONG Community with over 26,000 members Theme Music for Episode Promo Music: Your Love by Atch License: Creative Commons License - Attribution 3.0

In this episode, Kyle and Nate discuss the speaker shares advice on how to reduce visceral fat. They recommend trying a coffee enema to regain insulin sensitivity and dropping sugar consumption. Additionally, they discuss how stress and inflammation can lead to weight gain and health problems. The speaker also shares their keto success story. Regain insulin sensitivity, and decrease sugar consumption. They also suggest fasting to help improve insulin sensitivity and lose weight.   [00:00 - 05:52] Is Visceral Fat a Parasite or Parasitic Relationship? Sustainable weight loss is important Sustainable weight loss happens when there is more flexibility in one's lifestyle. Pop quiz!   [05:53 - 08:14] How to Cut Visceral Fat for a Healthier You Visceral fat: active fat that goes underneath the skin, underneath muscles, and around internal organs Endothelial dysfunction: impaired blood flow in the stomach and around organs Organ fat: fat that protects us from organ fat and leads to death if too much is accumulated Cytokines: inflammatory compounds that circulate in the body   [08:15 - 13:16] How to Lose Visceral Fat and Regain Insulin Sensitivity Do your waist to height ratio to determine if you have too much visceral fat Decrease your sugar consumption Start fasting Sustainability is key!   [13:17 - 22:40] How to Release Fat When You're Stressed Decrease toxins in the environment, reduce stress and consume healthy foods. Reducing visceral fat is a key part of reducing inflammation and improving overall health. There is a lot of stress in people's lives, and this can lead to inflammation. To reduce inflammation and improve weight loss, it is important to balance stress and recovery. Some ways to improve stress and recovery include massage, stretching, foam rolling, yoga, meditation, walking, and eating fruits and vegetables.   [22:41 - 29:45] How to Lose Visceral Fat: 3 Simple Tips To lose weight, focus on proteins and fats, especially early in the day when you're moving around. To regain insulin sensitivity, reduce sugar intake and increase walking. Give your body a reason to mobilize and burn fat by decreasing sugar intake and increasing walking. How to Lose Weight without Losing Your Mind   [29:46 - 31:58] Closing Segment The Food and Drug Administration (FDA) may not be giving consumers all the incentives they need to make healthy choices. Check out the free SugarDetox.com to help them start to get more insulin sensitive and free from insulin resistance.   Get lean, get strong, and get paid!     Let me help you reach your goals for FREE, just go to GetNatesBook.Com.  Break your relationship with sugar and drop fat in 5 days at SugarDetox.com, your first step to health. Learn more by connecting with me through Instagram or visit www.LowCarbHustlePodcast.com.      If you liked the show, please LEAVE A 5-STAR REVIEW, like, and subscribe through your favorite streaming platform!     Key Quote   "Whatever you're doing, however, you're trying to get rid of your visceral fat or trying to lose weight, it has to be sustainable." - Kyle Tyler

Watch the full video interview on YouTube here: https://bit.ly/drlouisignarro463 Dr. Louis Ignarro (IG: @dr.louisignarro) has more than 40 years of experience as a research scientist and was a professor at UCLA. In 1998, he was awarded a Nobel Prize for his groundbreaking discovery of nitric oxide or (NO) and its many critically important biological actions. Dr. Lou's groundbreaking research on NO paved the way for the drug Viagra and he's been nicknamed the Father of Viagra. Earlier this year Dr. Lou released a new book, Dr. NO: The Discovery That Led to a Nobel Prize and Viagra. NO is a molecule you may not have heard of before but it's powerful and can have a profound influence on your health - stick around to learn more. In this episode, we discuss: Why nitric oxide (NO) is important Specific foods that boost NO production How quickly does NO boost in your system after eating specific foods? Foods that cause a decrease in NO Arginine is required for nitric oxide production Specific functions NO has on the gastrointestinal (GI) system & bladder The physiology of nitric oxide for penile erection The rise of Viagra Dr. Lou recalls how he found out he won the Nobel Prize How winning the Nobel Prize changed his life personally & professionally Taking arginine & citrulline together boosts NO NO improves your athletic performance Endothelial cells produce NO when you exercise Breathing through your nose boosts NO in your lungs How breathing through your nose can protect against COVID-19 Hydration, rest, and electrolytes Eating a healthy, balanced diet Dr. Lou's passion for teaching & educating The future of NO research Show sponsors: BiOptimizers