Podcasts about immunoglobulin

Episode 190: Measles BasicsFuture Dr. Kapur explained the basics of measles, including the pathophysiology, diagnosis and management of this disease. Dr. Schlaerth added information about SPPE and told interesting stories of measles. Dr. Arreaza explained some statistics and histed the episode.  Written by Ashna Kapur MS4 Ross University School of Medicine. Comments by Katherine Schlaerth, MD, and Hector Arreaza, MD.You are listening to Rio Bravo qWeek Podcast, your weekly dose of knowledge brought to you by the Rio Bravo Family Medicine Residency Program from Bakersfield, California, a UCLA-affiliated program sponsored by Clinica Sierra Vista, Let Us Be Your Healthcare Home. This podcast was created for educational purposes only. Visit your primary care provider for additional medical advice.Introduction.According to the CDC, as of April 24, 2025, a total of 884 confirmed measles cases were reported by 30 states, including California, and notably Texas. This is already three times more cases than 2024. There are 3 confirmed deaths so far in the US. What is measles?Measles is a disease that's been around for centuries, nearly eradicated, yet still lingers in parts of the world due to declining vaccination rates. Let's refresh our knowledge about its epidemiology, clinical features, diagnosis, management, and most importantly — prevention.Definition.Measles, also known as rubeola, is an acute viral respiratory illness caused by the measles virus. It's a single-stranded, negative-sense RNA virus belonging to the Paramyxoviridae family. It's extremely contagious with a transmission rate of up to 90% among non-immune individuals when exposed to an infected person.EpidemiologyBefore the introduction of the measles vaccine in 1963, nearly every child got measles by the time they were 15 years old. With the introduction of vaccination, cases and deaths caused by measles significantly declined. For example, in 2018, over 140,000 deaths were reported in the whole world, mostly among children under the age of 5.Measles is still a common disease in many countries, including in Europe, the Middle East, Asia, and Africa. Measles outbreaks have been reported recently in the UK, Israel, India, Thailand, Vietnam, Japan, Ukraine, the Philippines, and more recently in the US. So, let's take prevention seriously to avoid the spread of this disease here at home and abroad. How do we get measles, Ashna?Mode of Transmission:● Air: Spread primarily through respiratory droplets.● Surfaces: The virus remains viable on surfaces or in the air for up to 2 hours. (so, if a person with measles was in a room and you enter the same room within 2 hours, you may still get measles)● Other people: Patients are contagious from 4 days before until 4 days after the rash appears.PathophysiologyThe measles virus first infects the respiratory epithelium, replicates, and then disseminates to the lymphatic system.It leads to transient but profound immunosuppression, which is why secondary infections are common. It affects the skin, respiratory tract, and sometimes the brain, leading to complications like pneumonia or encephalitis.Clinical PresentationThe classic presentation of measles can be remembered in three C's:● Cough● Coryza (runny nose)● ConjunctivitisCourse of Disease (3 Phases):1. Prodromal Phase (2-4 days)○ High fever (can peak at 104°F or 40°C)○ The 3 C's○ Koplik spots: Small white lesions on the buccal mucosa.2. Exanthem Phase○ Maculopapular rash begins on the face (especially around the hairline), then spreads from head to toe. The rash typically combines into 1 big mass as it spreads, and the fever often persists during the rash.3. Recovery Phase○ Rash fades in the same order it appeared.○ Patients remain at risk for complications during and after rash resolution.Complications:● Pneumonia (most common cause of death in children)● Otitis media (most common overall complication)● Encephalitis (can lead to permanent neurologic sequelae)● Subacute sclerosing panencephalitis (SSPE): A rare, fatal, degenerative CNS disease that can occur years after measles infection.High-risk groups for severe disease include:● Infants and young children● Pregnant women● Immunocompromised individualsDiagnosisClinical diagnosis is sufficient if classic symptoms are present, especially in outbreak settings.Ashna: Laboratory confirmation:● Measles-specific IgM antibodies detected by serology.● RT-PCR from nasopharyngeal, throat, or urine samples.Notify public health authorities immediately upon suspicion or diagnosis of measles to limit spread. ManagementThere is no specific antiviral treatment for measles. Management is supportive:● Hydration (by mouth and only IV in case of severe dehydration)● Antipyretics (e.g., acetaminophen) for fever● Oxygen if hypoxicVitamin A supplementation:● Recommended for all children with acute measles, particularly in areas with high vitamin A deficiency. It has shown to reduce morbidity and mortality.Hospitalization may be necessary for:● Severe respiratory compromise● Dehydration● Neurologic complicationsPrevention: We live in perilous times and vaccination is under scrutiny right now. Before the measles vaccine, about 48,000 people were hospitalized and 400–500 people died in the United States every year. Measles was declared eradicated in the US in 2000, but the vaccination coverage is no longer 95%. How do we prevent measles?Vaccination is the cornerstone of prevention.● MMR vaccine (Measles, Mumps, Rubella):○ First dose at 12-15 months of age.○ Second dose at 4-6 years of age.○ 97% effective after 2 doses.The Advisory Committee on Immunization Practices (ACIP) has noted that febrile seizures typically occur 7 to 12 days after vaccination with MMR, with an estimated incidence of 3.3 to 8.7 per 10,000 doses. The Centers for Disease Control and Prevention (CDC) states that febrile seizures following MMR vaccination are rare and not associated with any long-term effects. The risk of febrile seizures is higher when the MMR vaccine is administered as part of the combined MMRV (measles, mumps, rubella, and varicella) vaccine compared to the MMR vaccine alone.Post-exposure prophylaxis:● MMR vaccine within 72 hours of exposure (if possible).● Immunoglobulin within 6 days for high-risk individuals (e.g., infants, pregnant women, immunocompromised).Herd immunity requires at least 95% vaccination coverage to prevent outbreaks.Key Takeaways● Measles is a highly contagious viral illness that can lead to severe complications.● Diagnosis is often clinical, but lab confirmation helps with public health tracking.● Treatment is mainly supportive, with Vitamin A playing a critical role in reducing complications.● Vaccination remains the most effective tool to eliminate measles worldwide.While measles might seem like a disease of the past, it can make a dangerous comeback without continued vigilance and vaccination efforts.Even without trying, every night you go to bed a little wiser. Thanks for listening to Rio Bravo qWeek Podcast. We want to hear from you, send us an email at RioBravoqWeek@clinicasierravista.org, or visit our website riobravofmrp.org/qweek. See you next week! _____________________References:Centers for Disease Control and Prevention (CDC). Measles (Rubeola), Clinical Overview, July 15, 2024. Accessed on May 1, 2025. https://www.cdc.gov/measles/hcp/clinical-overview/index.html.World Health Organization (WHO). Measles, November 14, 2024. https://www.who.int/news-room/fact-sheets/detail/measlesGans, Hayley and Yvonne A. Maldonado, Measles: Clinical manifestations, diagnosis, treatment, and prevention, UpToDate, January 15, 2025. Accessed on May 1, 2025. https://www.uptodate.com/contents/measles-clinical-manifestations-diagnosis-treatment-and-preventionTheme song, Works All The Time by Dominik Schwarzer, YouTube ID: CUBDNERZU8HXUHBS, purchased from https://www.premiumbeat.com/.

The May 2025 recall features four previously posted episodes on myasthenia gravis. The first episode has Dr. Fredrik Piehl discussing rituximab for new-onset generalized MG. In the second episode, Dr. Vera Bril explores the potential use of immunoglobulin as a corticosteroid-sparing agent in MG patients. The third episode features Dr. Ali A. Habib discussing trends in hospital admissions and in-hospital mortality for adult MG patients. The series concludes with Dr. Jennifer Morganroth addressing the increase in thymectomy procedures post-MGTX trial, disparities in access to these surgeries among different demographic groups, and the rise of minimally invasive surgical techniques. Podcast links: Efficacy and Safety of Rituximab for New-Onset Generalized Myasthenia Gravis  Corticosteroid-Sparing Effects of Immunoglobulin in Myasthenia Gravis Hospitalizations and Mortality from MG Hospitalizations and Mortality From Myasthenia Gravis Trends and Disparities in the Utilization of Thymectomy for MG in the US Article links: Efficacy and Safety of Rituximab for New-Onset Generalized Myasthenia Gravis Randomized Double-Blind Placebo-Controlled Trial of the Corticosteroid-Sparing Effects of Immunoglobulin in Myasthenia Gravis Hospitalizations and Mortality From Myasthenia Gravis Trends and Disparities in the Utilization of Thymectomy for Myasthenia Gravis in the United States Disclosures can be found at Neurology.org.

In this episode, we discuss the relationship between the intestinal mucus layer and secretory immunoglobulin A (sIgA), highlighting both as key components of the gut's first line of defense against pathogens, toxins, and environmental stressors. We detail how dysbiosis and microbial overgrowths can impact sIgA levels and can disrupt mucus integrity and function, contributing to increased intestinal permeability and inflammation. We then examine several additional factors influencing sIgA production. Topics: 1. Introduction: Importance of Secretory IgA and Intestinal Mucus     - Intestinal epithelial lining and its protective mucus layer     - Focus on intestinal dysbiosis and sIgA   2. Anatomy of the Gastrointestinal Lining     - Intestinal lumen, microbiome, mucus layer, epithelial cells, tight junctions, lamina propria, and smooth muscle     - Role of goblet cells in mucus secretion and epithelial integrity    3. Role of the Protective Mucus Layer     - Dual function of the mucus layer as a physical and biochemical barrier     - Composition of mucus: mucins, antimicrobial peptides, immunoglobulins    4. Goblet Cells and Mucin Production     - Mechanisms of mucin secretion and hydration     - Variability in goblet cell density across the intestinal tract     - Dysfunctions linked to conditions like IBD and IBS    5. Microbial Interactions with Mucins     - Glycan structures in mucins    - Roles of commensal and pathogenic bacteria in mucin degradation and mucus turnover     - Importance of regulated mucus production for gut homeostasis    6. Dysbiosis and Mucus Degradation     - Excessive mucin degradation leading to thinning of the mucus layer     - Consequences of increased intestinal permeability and inflammation     - Role of dysbiosis in disrupting the mucus layer    7. Overview of Secretory IgA     - Introduction to secretory IgA (sIgA) and its role in mucosal immunity    - Mechanisms of immune exclusion and pathogen neutralization    8. Production and Function of Secretory IgA     - sIgA production by plasma cells in the lamina propria     - Translocation of IgA through epithelial cells into the gut lumen     - sIgA binding to mucins and its immunological role in the mucus layer    9. Factors Affecting Secretory IgA Levels     - Possible contributing factors to low sIgA levels    - Implications of low and high sIgA levels for immune defense and intestinal health    10. Interactions Between Secretory IgA and Dysbiosis     - Cyclical relationship between low sIgA, dysbiosis, and infections     - Examples of overgrowths, like candida, disrupting sIgA     - Importance of addressing immune dysregulation to break the cycle    11. Bioindividual Root Cause Approach  - Factors to consider Thank you to our episode sponsors: 1. Shop ⁠⁠Fresh Press Farms⁠⁠' Peach Cider Vinegar at Sprouts locations nationwide, and check out their full collection ⁠⁠here⁠⁠. 2. Check out ⁠Spectrum Sciences⁠' Serums and The Vitamin B12 Protocol ⁠here⁠, and use code CHLOE15 for 15% off. Get Chloe's Book Today! "⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠75 Gut-Healing Strategies & Biohacks⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠" Follow Chloe on Instagram ⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠@synthesisofwellness⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠ Visit ⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠synthesisofwellness.com⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠

In this episode, we go through the gut-immune axis and mycobiome, focusing on the mechanisms underlying intestinal fungal overgrowth. We highlight the roles of the intestinal mucus layer, intestinal epithelial cells, lamina propria, and secretory immunoglobulin A (sIgA) in maintaining homeostasis and defending against fungal overgrowth. We also detail the pathogenic transition of Candida from its commensal yeast form to an invasive hyphal state, as well as the resulting disruptions to immune function. Topics 1. Introduction to the Gut-Immune Axis and Mycobiome    - The gut-immune axis and mycobiome.    - Exploring intestinal fungal overgrowth.   2. Overview of the Gastrointestinal Lining    - Anatomy of the intestinal lining: lumen and microbiome, mucus layer, epithelial monolayer, and lamina propria.    - The dual role of the mucus layer: physical and biochemical barrier.    - Composition of mucus.   3. Goblet Cells and Mucus Production    - Goblet cells as critical producers of mucin glycoproteins and their role in mucus barrier integrity.    - Signals for mucin release, such as mechanical stress.   4. The Lamina Propria and Gut-Associated Lymphoid Tissue (GALT)    - Layers beneath the epithelium    - Peyer's patches, mesenteric lymph nodes, isolated lymphoid follicles, and intraepithelial lymphocytes.    - Immune tolerance and defense against pathogens.   5. Peyer's Patches    - Positioning of Peyer's patches.    - The transition of microbial density from the small intestine to the colon and its immune implications.   6. Immune Cells in the Lamina Propria    - Overview of macrophages, dendritic cells, mast cells, plasma cells, and lymphocytes.    - Their role in maintaining immunological equilibrium in the gut.   7. The Mycobiome and Pathogenic Transition of Candida    - Candida's transition from yeast to hyphal form.    - Regulation of Candida by microbial competition and immune surveillance.    - Factors promoting pathogenicity: immune suppression, lacking beneficial microbes, more.   8. Virulence Factors of Candida    - Secretion of hydrolytic enzymes such as SAPs and phospholipases.    - Degradation of sIgA and its consequences for immune evasion.    - Adhesins that facilitate fungal binding.   9. Intestinal Barrier Disruption by Candida    - Penetration of the mucus layer and epithelial barrier by Candida hyphae.    - Disruption of tight junctions and the resulting increased intestinal permeability.    - Inflammatory cascade triggered by fungal antigens and microbial toxins entering the lamina propria.   10. Biofilm Formation     - The role of biofilms in shielding fungal cells from immune attack and antifungal treatments.   11. Small Intestinal Fungal Overgrowth (SIFO)     - Intestinal dysmotility, low stomach acid, immunocompromise.     - Possible symptoms of SIFO.     - Discussion of the overlap between SIFO, SIBO, histamine intolerance, and other gastrointestinal conditions. - Root cause. Thank you to our episode sponsors: 1. Shop ⁠Fresh Press Farms⁠' Peach Cider Vinegar at Sprouts locations nationwide, and check out their full collection ⁠here⁠. 2. Check out ⁠⁠⁠⁠⁠Ulyana Organics'⁠⁠⁠⁠⁠ ⁠⁠⁠⁠⁠Tallow Wild Yam Cream⁠⁠⁠⁠⁠ and ⁠⁠⁠⁠⁠Healing Facial Oil⁠⁠⁠⁠⁠, and use code ⁠⁠⁠⁠⁠CHLOE10⁠⁠⁠⁠⁠ 10% off your order. 3. Check out Spectrum Sciences' Serums and The Vitamin B12 Protocol here, and use code CHLOE15 for 15% off. Get Chloe's Book Today! "⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠75 Gut-Healing Strategies & Biohacks⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠" Follow Chloe on Instagram ⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠@synthesisofwellness⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠ Visit ⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠synthesisofwellness.com⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠⁠

Today, Fei and Nick are joined by Dr. Sarah Prager and Dr. Sarah Horvath to talk about the new recommendations for Rh immunoglobulin administration in early pregnancy. Should we still be giving it for bleeding in early pregnancy? If so, how much?  Dr. Sarah Prager is a Professor of Obstetrics and Gynecology and Division Chief of Complex Family Planning at the University of Washington Medical Center.  Dr. Sarah Horvath is an Associate Professor of Obstetrics and Gynecology and Vice Chair of Research at Penn State.  Twitter: @creogsovercoff1 Instagram: @creogsovercoffee Facebook: www.facebook.com/creogsovercoffee Website: www.creogsovercoffee.com Patreon: www.patreon.com/creogsovercoffee You can find the OBG Project at: www.obgproject.com

The Balchem technical team selected abstracts of interest from the 2024 American Dairy Science Association meetings to feature on this episode of the Real Science Exchange. Whole Cottonseed and Fatty Acid Supplementation Affect Production Responses During the Immediate Postpartum in Multiparous Dairy CowsGuests: Jair Parales-Giron and Dr. Adam Lock, Michigan State University (0:58)The experiment had four treatment groups: no fat supplement, 10% of the diet from whole cottonseed, a 60:30 mix of calcium salts of palmitic and oleic acid at 1.5% of the diet dry matter, and a combination of both whole cottonseed and fatty acid supplement. Energy-corrected milk was increased by almost six kilograms in cows fed the whole cottonseed diet, with a similar increase of more than five kilograms in the fatty acid-supplemented cows during the first 24 days of lactation. However, no further improvement was observed when both whole cottonseed and fatty acids were fed together. The increase in milk production was not accompanied by increased weight loss or loss of body condition. Effect of Close-Up Metabolizable Protein Supply on Colostrum Yield, Composition, and Immunoglobulin G ConcentrationGuests: Dr. Trent Westhoff and Dr. Sabine Mann, Cornell University (17:06)In this study, cows were assigned to one of two diets 28 days before expected calving: one that provided 39 grams of metabolizable protein (MP) per pound of dry matter and one that supplied 51 grams of MP per pound of dry matter. This represents about 100% of the MP requirement and 140% of the MP requirement, respectively. Diets were formulated to supply equal amounts of methionine and lysine. Cows entering their second parity who were fed the elevated MP diet produced two liters more colostrum than second parity cows fed the control MP diet. This effect was not observed in cows entering their third or higher parity. Overall, higher MP supply did not impact colostrum quantity or quality. Dr. Westhoff also highlights an invited review he authored regarding nutritional and management factors that influence colostrum production and composition. The MP research has also been published; links to both are below.MP paper: https://www.sciencedirect.com/science/article/pii/S0022030224010774Invited review: https://www.sciencedirect.com/science/article/pii/S0022030224000341Colostrum—More than Immunoglobulin G (IgG): Colostrum Components and Effects on the CalfGuest: Dr. Sabine Mann, Cornell University (41:23)Dr. Mann presented this abstract at an ADSA symposium titled “Colostrum: The Role It Plays In Calf Health, Development, and Future Productivity.”  Her focus was to give credit to the importance of IgG while reminding the symposium audience of the importance of other colostrum components like bioactive factors and nutrients. There is potential that measuring IgG could be a marker for all the other colostrum components that have been transferred as well. We have excellent and cost-effective ways to measure IgG calf-side, but very few bioactive factors can be measured as easily. Heat treatment of colostrum to control bacterial contamination has a detrimental effect on many of the non-IgG components of colostrum. More data is needed to learn how impactful this may be to the calf. Dr. Mann details parts of the heat treatment process that farmers can check to make sure heat treatment is having as little impact as possible. She also would like to have a way to measure the antimicrobial activity of colostrum and the concentrations of insulin and IGF-1 in colostrum on-farm. Lastly, she reminds the audience that we can focus a lot on making the best quality colostrum via transition cow management and best management practices for colostrum harvest, but we still need to get it into the calf. Colostrum must get into calves cleanly and safely, at an adequate amount, and at an optimal temperature.Please subscribe and share with your industry friends to invite more people to join us at the Real Science Exchange virtual pub table.  If you want one of our Real Science Exchange t-shirts, screenshot your rating, review, or subscription, and email a picture to anh.marketing@balchem.com. Include your size and mailing address, and we'll mail you a shirt.

Ever heard of immunoglobulins? While colostrum is experiencing a renaissance, you should seriously weigh the immunoglobulin supplement versus colostrum benefits.Partly because colostrum is a LOWER potency option, that sometimes isn't even standardized (depending on the brand) to guarantee how helpful it will be.That said, immunoglobulins (aka. IgGs) can be incredibly helpful for things like leaky gut (gut permeability), histamine intolerance, recovering from a stomach bug or food poisoning, and supporting your immune system during cold + flu season.Plus, I'm going to share why I prefer immunoglobulin supplements over colostrum, what the difference is between the two, and how to use them.Also, I want to how you how to avoid the fake, over-hyped colostrum + immunoglobulin supplements found online (even on Amazon)!Let's dive in!In This Episode:How I learned about the amazing benefits of immunoglobulin supplementWhy you should use immunoglobulinsDifference between immunoglobulins and colostrum benefitsHow do immunoglobulins work?Benefits of immunoglobulinsHow to take an immunoglobulin supplementBest immunoglobulin supplement + where to get itQuotes“[Immunoglobulins] were traditionally used and still are actually for more diarrhea-dominant problems. So if you've got a lot of loose soft stools, diarrhea, or even something like inflammatory bowel disease.”“IgGs can be found in the serum of your blood, and they also can be found in the serum of the blood of cows. And so historically, when people supplement with colostrum, that's derived from dairy, and it contains IgGs. So they are similar in that colostrum has IgGs and it's one of the major components that makes it so helpful, but it's not as potent.”LinksGET REAL, VERIFIED IMMUNOGLOBULINS HEREHealthy Skin Show ep. 188: Histamine Intolerance + Skin Rashes (PART 1)Healthy Skin Show ep. 192: Histamine Intolerance + Skin Rashes, PART 2

Oncimmune Holdings CEO Martin Gouldstone joined Steve Darling from Proactive to provide an encouraging trading update on the company's recent commercial progress. Over the last quarter, Oncimmune has continued its positive momentum, signing three new contracts valued at £0.34 million. These add to an impressive streak, bringing the total to eight contracts worth £2.14 million secured in just three months. Two of the latest agreements mark a significant expansion, as they involve new work areas for an existing top 10 global pharmaceutical client, highlighting Oncimmune's growing versatility and value to major industry players. The third contract leverages Oncimmune's expertise in profiling Immunoglobulin type E (IgE) autoantibodies, further solidifying its relationship with another top-tier pharmaceutical partner. Though these contracts do not prompt an increase in the FY2025 revenue outlook, they strengthen Oncimmune's position to meet market expectations. The company now has visibility of over 40% of its FY2025 revenue target of £6.9 million, supported by a robust, high-confidence pipeline. This ongoing success underscores Oncimmune's expanding commercial traction and its position as a trusted partner to leading pharmaceutical companies worldwide. #proactiveinvestors #oncimmuneholdingsplc #aim #onc.l #AutoantibodyProfiling #PharmaContracts #BiotechNews #IgEInnovation #Immunology #AutoimmuneDisease #CancerResearch #PharmaIndustry #ProactiveInvestors #MedicalResearch #invest #investing #investment #investor #stockmarket #stocks #stock #stockmarketnews

Learn about Serum Bovine Immunoglobulin (SBI) Powder, a game-changer in gut health with Brian Kaufman, RN, Vice President of Global Business Development for Proliant Health & Biologicals. By selectively removing gut pathogens without disturbing commensal bacteria, SBIs help in conditions as diverse as SIBO, IBS, Candida, Crohn's, colitis, malnutrition, leaky gut, cancer, HIV, psoriasis and acute gut infections like C Difficile. Learn how this plasma-derived bovine immunoglobulin (IgG) supplement facilitates gut healing and improves nutrient absorption while preserving the microbiome. Lindsey Parsons, your host, helps clients solve gut issues and reverse autoimmune disease naturally. Take her quiz to see which stool or functional medicine test will help you find out what's wrong. She's a Certified Health Coach at High Desert Health in Tucson, Arizona. She coaches clients locally and nationwide. You can also follow Lindsey on Facebook, Tiktok, X, Instagram or Pinterest or reach her via email at lindsey@highdeserthealthcoaching.com to set up a free 30-minute Gut Healing Breakthrough Session. Show Notes

Here are the most prominent News Highlights of Sri Lanka. Manoj Udatiyawala reports - SBS සිංහල සේවය ගෙනෙන "මවුබිමෙන් පුවත්" - මාධ්‍යවේදී මනෝජ් උදටියාවල ශ්‍රී ලංකාවේ සිට වාර්තා කරයි.

Martin van Hagen, MD, PhD - Patients Receiving Immunoglobulin Therapy: Emerging Technology for Patient-Integrated Care

Martin van Hagen, MD, PhD - Patients Receiving Immunoglobulin Therapy: Emerging Technology for Patient-Integrated Care

Martin van Hagen, MD, PhD - Patients Receiving Immunoglobulin Therapy: Emerging Technology for Patient-Integrated Care

Martin van Hagen, MD, PhD - Patients Receiving Immunoglobulin Therapy: Emerging Technology for Patient-Integrated Care

Dr. Corey Watson is an Associate Professor at the University of Louisville. His work focuses on characterising and cataloguing antibody genetic diversity in human and mouse to better understand disease susceptibility and clinical health outcomes. Dr. William Lees is a researcher at University of London. His work focuses on developing Adaptive Immune Receptor (AIR) reference sets for diverse species and the annotation of experimental sequence data. In this episode we talk about the recent work by the Germline Database Working Group of the AIRR-Community. The accuracy of V and J gene segment assignment improves with the quality of the reference germline set. The accurate assignment is critical for characterization of somatic hypermutation. We discuss the challenges in creating a database to hold all relevant and potentially relevant germline information, especially in the light of increased discovery rate through technological advances and improved analysis pipelines. We also reflect on the complexity in handling personalised germline reference sets. The episode is hosted by Dr. Ulrik Stervbo and Dr. Zhaoqing Ding. Comments are welcome to the inbox of onairr@airr-community.org  or on social media under the tag #onAIRR. Further information can be found here: https://www.antibodysociety.org/the-airr-community/airr-c-podcast.  Website of the AIRR-C Germline Database Working Group https://www.antibodysociety.org/the-airr-community/airr-working-groups/germline_database/  Papers mentioned Collins, Andrew M., Mats Ohlin, Martin Corcoran, James M. Heather, Duncan Ralph, Mansun Law, Jesus Martínez-Barnetche, et al. 2023. “AIRR-C Human IG Reference Sets: Curated Sets of Immunoglobulin Heavy and Light Chain Germline Genes.” BioRxiv. https://doi.org/10.1101/2023.09.01.555348  Rodriguez, Oscar L., Yana Safonova, Catherine A. Silver, Kaitlyn Shields, William S. Gibson, Justin T. Kos, David Tieri, et al. 2023. “Genetic Variation in the Immunoglobulin Heavy Chain Locus Shapes the Human Antibody Repertoire.” Nature Communications 14 (1). https://doi.org/10.1038/s41467-023-40070-x  Lees, William D., Scott Christley, Ayelet Peres, Justin T. Kos, Brian Corrie, Duncan Ralph, Felix Breden, et al. 2023. “AIRR Community Curation and Standardised Representation for Immunoglobulin and T Cell Receptor Germline Sets.” Immunoinformatics (Amsterdam, Netherlands) 10 (100025): 100025. https://doi.org/10.1016/j.immuno.2023.100025  Jackson, Katherine J. L., Justin T. Kos, William Lees, William S. Gibson, Melissa Laird Smith, Ayelet Peres, Gur Yaari, et al. 2022. “A BALB/c IGHV Reference Set, Defined by Haplotype Analysis of Long-Read VDJ-C Sequences From F1 (BALB/c x C57BL/6) Mice.” Frontiers in Immunology 13. https://doi.org/10.3389/fimmu.2022.888555  Ford, Easton E., David Tieri, Oscar L. Rodriguez, Nancy J. Francoeur, Juan Soto, Justin T. Kos, Ayelet Peres, et al. 2023. “FLAIRR-Seq: A Method for Single-Molecule Resolution of near Full-Length Antibody H Chain Repertoires.” The Journal of Immunology 210 (10): 1607–19. https://doi.org/10.4049/jimmunol.2200825  Omer, Aviv, Ayelet Peres, Oscar L. Rodriguez, Corey T. Watson, William Lees, Pazit Polak, Andrew M. Collins, and Gur Yaari. 2022. “T Cell Receptor Beta Germline Variability Is Revealed by Inference from Repertoire Data.” Genome Medicine 14 (1). https://doi.org/10.1186/s13073-021-01008-4  Rodriguez, Oscar L., Catherine A. Silver, Kaitlyn Shields, Melissa L. Smith, and Corey T. Watson. 2022. “Targeted Long-Read Sequencing Facilitates Phased Diploid Assembly and Genotyping of the Human T Cell Receptor Alpha, Delta, and Beta Loci.” Cell Genomics 2 (12): 100228. https://doi.org/10.1016/j.xgen.2022.100228  Tools mentioned TIgGER (Immcantation) https://tigger.readthedocs.io/en/stable  IgDiscover https://github.com/NBISweden/IgDiscover  Partis https://github.com/psathyrella/partis MiXCR https://mixcr.com

Did you know that modulation of the neonatal Fc receptor (FcRn) can improve outcomes for patients with immunoglobulin G-mediated autoimmune diseases?    Credit available for this activity expires: 11/08/2024 Earn Credit / Learning Objectives & Disclosures: https://www.medscape.org/viewarticle/995314?ecd=bdc_podcast_libsyn_mscpedu

Are you struggling with food sensitivities but don't want to eliminate your favorite foods? I've got good news for you! In this episode of the Love Your Gut podcast, I had the pleasure of speaking with Brian Kaufman, an expert in digestive health and infectious disease, about how to improve your food sensitivities without having to cut out all the foods you love. Discover how you can achieve the outcome you desire – finding relief from food sensitivities – while still enjoying a wide variety of delicious meals. We dive into the fascinating world of food sensitivities, discussing practical strategies and tips to alleviate symptoms while still enjoying a diverse diet. If you're tired of feeling restricted by your food sensitivities, this episode is a must-listen! Topics covered in this episode: Deep dive the role of serum bovine immunoglobulin in promoting a healthy digestive system.Learn the vital role of immunoglobulins in boosting your body's defenses.Uncover the hidden connection between serum bovine immunoglobulin and the improvement of leaky gut symptoms.Grasp the complex connection between stress and its impact on gut health.Get to know how Immunolin effectively aids in managing and improving digestive health conditionsRecommendations:LMNT electrolytesPurchase Immunogoblin products hereFollow Brian Kauffman:Instagram: https://www.instagram.com/immunolin/Website: https://phb1.com/Follow Dr. HeatherInstagram: https://www.instagram.com/drheatherfinley/Website: https://www.drheatherfinley.co/podcast/KEY MOMENTS:04:57 - The Benefits of Immunoglobulin,09:34 - Importance of Gut Health,10:42 - Why Immunoglobulin is Needed Now,15:27 - Understanding the Safety and Dosing of a Protein Supplement,17:42 - Clinical Efficacy and Dosage for Specific Disease States,19:06 - Personal Experience and Benefits of Immunolin,24:54 - Immunolin and its Binding Properties,30:07 - Accessing the Product,31:15 - Other Products,32:59 - IGGI Shield,34:56 - Positive Implications,36:58 - Life Begins in the Gut, 

Did you know that immunoglobulin A nephropathy has very recently seen 2 treatments first licensed for its management? Credit available for this activity expires: 6/29/24 Earn Credit / Learning Objectives & Disclosures: https://www.medscape.org/viewarticle/993766?ecd=bdc_podcast_libsyn_mscpedu

Không chỉ phải đối mặt với tình trạng căng thẳng của dịch tay chân miệng khi đã xuất hiện trường hợp tử vong, nhiều ca nặng ở các địa phương phải chuyển về tuyến cuối và nguy cơ dịch chồng dịch, TP.HCM còn phải đối mặt với nguy cơ thiếu thuốc điều trị. Hiện các loại thuốc Immunoglobulin, Pentaglobin (chế phẩm từ huyết tương) và Phenobarbital đang khan hiếm nên việc điều trị gặp nhiều khó khăn.

Warm autoimmune hemolytic anemia (wAIHA) is the most common type (60-70%) of autoimmune hemolytic anemia (AIHA). In most cases, wAIHA is due an immunoglobulin G (IgG) autoantibody that binds to red blood cells (RBC), leading to hemolysis. Current recommendations for managing people with wAIHA are largely based on case series and retrospective studies involving off-label medications. Also, while there are currently no medications specifically approved to treat wAIHA, data are emerging on new therapies under investigation which may impact treatment in the future. This 60-minute CME program, hosted by Irina Murakhovskaya, MD, of the Montefiore Medical Center, Albert Einstein College of Medicine, in New York, NY and Bruno Fattizzo, MD, of the University of Milan and Fondazione IRCCS Ca' Granda Ospedale Maggiore Policlinico, in Milan, Italy, describes current best practices to manage patients with wAIHA.Supported by an educational grant from Janssen Biotech. For complete activity information and to obtain CME credit, please, go to www.checkrare.com

Nizar Mahlaoui, MD, MPH, PhD - Simplifying Patient-Integrated Care for Those Requiring Immunoglobulin Therapy: Making Smart Moves with Automated and Digital Technology

Nizar Mahlaoui, MD, MPH, PhD - Simplifying Patient-Integrated Care for Those Requiring Immunoglobulin Therapy: Making Smart Moves with Automated and Digital Technology

Nizar Mahlaoui, MD, MPH, PhD - Simplifying Patient-Integrated Care for Those Requiring Immunoglobulin Therapy: Making Smart Moves with Automated and Digital Technology

Nizar Mahlaoui, MD, MPH, PhD - Simplifying Patient-Integrated Care for Those Requiring Immunoglobulin Therapy: Making Smart Moves with Automated and Digital Technology

Nizar Mahlaoui, MD, MPH, PhD - Simplifying Patient-Integrated Care for Those Requiring Immunoglobulin Therapy: Making Smart Moves with Automated and Digital Technology

Nizar Mahlaoui, MD, MPH, PhD - Simplifying Patient-Integrated Care for Those Requiring Immunoglobulin Therapy: Making Smart Moves with Automated and Digital Technology

WARNING FOR PARENTS: In this episode, we talk about sex, so this would be a good one to listen to when your children are not in the car. SPOUSES: If you are married, we recommend listening to this episode WITH YOUR SPOUSE. If they are not with you, pause it and plan a time to listen together. In this episode, we talk about so many of the benefits of sexual intimacy in marriage. We talked about some of the hormones that are responsible for all of these benefits and wanted to share the notes on this for your reference: Endorphins: Endorphins released during orgasm actually mimic morphine Oxytocin: Known as the “love hormone” is released during an orgasm  Also released during labor and breastfeeding - it bonds a mother to her baby.   Causes feelings of attachment and trust Immunoglobulin a (IgA): These antibodies help combat disease and keep bodies safe from colds and flu Testosterone and Estrogen: Can keep the body looking young and vital   Neurotransmitters: Dopamine - related to how we feel pleasure and motivates our brains to feel that pleasure again  Seratonin - regulates mood, social behaviors, emotions, appetite, and digestion, sleep, memory, and sex drive.  Epinephrine: An adrenaline hormone which activates your sympathetic nervous system giving you feelings of exhilaration.   Nitric Oxide: Minimizes symptoms of depression and anxiety

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2023.03.27.534487v1?rss=1 Authors: Osaka, J., Ishii, A., Wang, X., Hakeda-Suzuki, S., Iwanaga, R., Kawamura, H., Suzuki, T. Abstract: Neurons express many cell surface proteins as mutually binding key-lock molecules that can create synapses. However, the molecular mechanisms of how neurons make synapses only with preferred targets are not completely understood. Here we identified Side-IV and Beat-IIb, belonging to the Drosophila immunoglobulin superfamily, as a new key-lock combination capable of inducing synapse formation. Side-IV interaction with Beat-IIb transduces bifurcated signaling to Side-IV's co-receptor, Kirre, and a synaptic scaffold protein, Dsyd-1. Localization and genetic interaction analyses revealed that Side-IV localizes subcellularly at synapse formations defined by Beat-IIa/b and anchors Dsyd-1.Our data demonstrate that a complex made up of Side-IV, Beat-IIb, Kirre, and Dsyd-1 not only narrows neuronal binding specificity but also recruits synapse formation factors Kirre and Dsyd-1 to restrict synapse formation loci and inhibit miswiring. We propose a mechanism by which key-lock molecules set a hierarchy of preference among neuronal pairs in a complex circuit in vivo. Copy rights belong to original authors. Visit the link for more info Podcast created by Paper Player, LLC

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2023.03.18.533291v1?rss=1 Authors: Ptak, C. P., Peterson, T. A., Hopkins, J. B., Ahern, C. A., Shy, M. E., Piper, R. C. Abstract: Mutations in Myelin Protein Zero (MPZ) account for 5% of Charcot-Marie-Tooth cases and can cause demyelinating or axonal phenotypes, reflecting the diverse roles of MPZ in Schwann cells. MPZ holds the apposing membranes of the myelin sheath together, with the adhesion role fulfilled by the extracellular Immunoglobulin-like domain (IgMPZ), which can oligomerize. Current knowledge for how the IgMPZ might form oligomeric assemblies involving 3 weakly-interacting interfaces has been extrapolated from a protein crystal structure in which individual rat IgMPZ subunits are packed together under artificial conditions. These interfaces include one that organizes the IgMPZ into tetramers, a 'dimer' interface that could link tetramers together, and a third hydrophobic interface that could mediate binding to lipid bilayers or the same hydrophobic surface on another IgMPZ domain. There are at present no data confirming whether the proposed IgMPZ interfaces actually mediate oligomerization in solution, whether they are required for the adhesion activity of MPZ, whether they are important for myelination, or whether their loss results in disease. We performed NMR and SAXS analysis of wild-type IgMPZ as well as mutant forms with amino-acid substitutions designed to interrupt its presumptive oligomerization interfaces. Here, we confirm the interface that mediates IgMPZ tetramerization, but find that dimerization is mediated by a distinct interface that has yet to be identified. We next correlated CMT phenotypes to subregions within IgMPZ tetramers. Axonal late-onset disease phenotypes (CMT2I/J) map to surface residues of IgMPZ proximal to the transmembrane domain. Early-onset demyelinating disease phenotypes (CMT1B/Dejerine-Sottas syndrome) map to two groups: one is described by variants that disrupt the stability of the Ig-fold itself and are largely located within the core of the Ig domain; whereas another describes a surface on the distal outer surface of IgMPZ tetramers. Computational docking studies predict that this latter disease-relevant subregion may mediate dimerization of IgMPZ tetramers. Copy rights belong to original authors. Visit the link for more info Podcast created by Paper Player, LLC

Dr. Vera Bril discusses her paper, "Randomized Double-Blind Placebo-Controlled Trial of the Corticosteroid-Sparing Effects of Immunoglobulin in Myasthenia Gravis".  Show references: https://doi.org/10.1212/WNL.0000000000201501

Dr. Gordon Smith talks with Dr. Vera Bril about exploring the potential use of immunoglobulin as a corticosteroid-sparing agent in patients with Myasthenia Gravis.  Read the related article in Neurology.

Nathan is 47 years old, married, and a father of 3. A decent high school athlete, he was always a hard gainer. He was a lightly recruited basketball player in TX but started gaining muscle weight when he went to college. He dropped out after some financial issues during his senior year and joined the Army in 1999. After learning Arabic, he got to Ft. Bragg just before 9/11 and spent the next 8 years going back and forth to Afghanistan and Iraq as a member of Delta Force. He left the Army in 2010, finished college, took a couple of MMA fights, and then served 5 years as an operative in the US Intelligence community. He left that in 2015 and has owned a couple of consulting businesses and done government-related Independent Contracting since then. He does some health and wellness coaching on the side. He has always worked out, stayed fit, and he was a stand-out "military athlete." He ate whatever he wanted, and stayed "fit" but dealt with injuries and "aging" related issues. Adult acne, radial neuropathy, premature balding, skin tags, signs of inflammation (gingivitis, tinnitus, plantar fasciitis, etc.). He found keto in 2016 and was blown away. He went carnivore in 2019 and was even further blown away. He was intensely sick and didn't know it until he had the benefit of hindsight with zero-carb. In August 2022, he developed Gullian-Barre Syndrome. They told him he'd be an inpatient for 6-8 weeks, do inpatient physical therapy for 3-4 months, and then do outpatient physical therapy for however long it took to regain the movement, feeling, strength, and endurance that he'd lost (assuming he ever regained it). He walked out of the hospital 2 weeks after he entered under his own power. He went to his first outpatient PT appointment the next day and the doctor told him not to come back because he couldn't do anything for him. It took a few weeks for some residual numbness to disappear, but he feels back to new. Some people think his diet put him in the hospital, but he knows his diet got him out faster than anyone on his treatment team could fathom. Timestamps: 00:00 Trailer 00:47 Introduction 03:08 Intensely sick, brainfog, ADHD 05:36 Guillain-Barre syndrome diagnosis 08:26 Guillain-Barre syndrome causes 10:08 Guillain-Barre syndrome progression 13:05 Lumbar puncture to diagnose Guillain-Barre syndrome 14:06 Immunoglobulin therapy 16:23 Making full recovery 18:32 Hospital food 20:41 Guillain-Barre prognosis 22:55 No residual symptoms 26:05 Outpatient neurologist's opinion: “abnormal case of Guillain-Barre” 27:45 Other things that improved on the carnivore diet 30:47 On the ketogenic diet 32:33 Current diet with family 36:25 LDL and old people's health 38:22 Obesity in children 40:34 ACL injuries in children 43:32 Demonizing red meat 45:00 Peak performance and meat 49:23 Closing See open positions at Revero: https://jobs.lever.co/Revero/ Join Carnivore Diet for a free 30 day trial: https://carnivore.diet/join/ Book a Carnivore Coach: https://carnivore.diet/book-a-coach/ Carnivore Shirts: https://merch.carnivore.diet Subscribe to our Newsletter: https://carnivore.diet/subscribe/ . ‪#revero #shawnbaker #Carnivorediet #MeatHeals #HealthCreation   #humanfood #AnimalBased #ZeroCarb #DietCoach  #FatAdapted #Carnivore #sugarfree  ‪

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2022.10.13.512159v1?rss=1 Authors: Chanez-Paredes, S. D., Abtahi, S., Zha, J., Zuo, L., He, W., Turner, J. R. Abstract: Intestinal epithelia express two long MLCK splice variants, MLCK1 and MLCK2. We have previously shown that disruption of inflammation-induced MLCK1 recruitment to the perijunctional actomyosin ring prevents barrier loss and attenuates disease progression. Here we sought to define the domains responsible for distinct MLCK1 and MLCK2 behaviors. Quantitative analysis of human biopsies demonstrated specific increases in MLCK1 expression and perijunctional localization in Crohns disease. When expressed in cultured intestinal epithelial cells, we found, as expected, that MLCK1 is most concentrated at the perijunctional actomyosin ring. In contrast, MLCK2 is predominantly associated with basal F-actin stress fibers. Immunoglobulin-cell adhesion molecule domain 3 (IgCAM3) must be critical for MLCK1 recruitment, as that domain is incomplete in MLCK2. Consistent with this, truncation mutants consisting of N-terminal IgCAM domains 1-4, without C-terminal catalytic domains, localized similarly to full-length MLCK1 and MLCK2, respectively. Further mutagenesis allowed identification of IgCAM2 and IgCAM3 domains as the minimal region required for MLCK1 recruitment. Although IgCAM3 does not concentrate perijunctionally, it can act as a dominant negative effector that limits steady-state and TNF-induced MLCK1 recruitment and barrier loss. Together, the demonstration of selective MLCK1 upregulation and perijunctional recruitment in Crohns disease and identification of domains required for perijunctional MLCK1 recruitment provide a conceptual understanding and structural data needed for development of therapeutic means of blocking MLCK1-mediated barrier loss without the toxicity of enzymatic MLCK inhibition. SIGNIFICANCE STATEMENTRecent work has demonstrated that long myosin light chain kinase isoform 1 (MLCK1) recruitment to the perijunctional actomyosin ring is a critical component of inflammation-induced intestinal barrier loss. Chanez-Paredes et al. show that this occurs in Crohns disease and define the essential structural elements that direct MLCK1 recruitment, thereby creating a foundation for therapeutic interruption of MLCK1 recruitment in disease. Copy rights belong to original authors. Visit the link for more info Podcast created by Paper Player, LLC

Paul shares the results from his August Bloodwork panel from Marek Health. He does a deep dive into Testosterone, Immunoglobulin, Boron, Genistein, Isoflavonoids and touches on TMAO, Uric acid, and GGT. A note from Paul: Throughout my training and practice as a physician I have come to one very disappointing conclusion:  Western medicine isn't helping people lead better lives. Now that I've realized this, I've become obsessed with understanding what makes us healthy or ill. I want to live the best life I can and I want to be able to share this knowledge with others so that they can do the same. This podcast is the result of my relentless search to understand the roots of chronic disease. If you want to know how to live the most radical life possible I hope you'll join me on this journey. Time Stamps: 00:09:43 Podcast begins 00:12:08 Paul's lipid panel  00:14:48 Is elevated LDL a risk factor for heart disease? 00:24:08 Paul's Plasma, Ammonia results 00:25:28 Paul's Anemia profile 00:32:08 Paul's Blood Count profile 00:34:08 Paul's Thyroid panel 00:36:53 Comprehensive metabolic panel 00:38:13 Paul's Urinalysis 00:40:13 Paul's hormones 00:42:53 Insulin sensitivity  00:47:30 Boron 00:59:58 How to optimize your testosterone and increase androgen receptors 01:03:18 The detriments of consuming soy 01:05:43 Paul's thoughts on TMAO 01:09:40 Paul reviews the remainder of his bloodwork Sponsors: Heart & Soil: www.heartandsoil.co Sign up for Animal Based Gathering 2023: animalbasedgathering.com  Make a donation to the Animal Based Nutritional Research Foundation: abnrf.org   Marek Health: marekhealth.com/fundamentalhealth, use code PAUL for 10% off your first lab order Primal Pastures: www.primalpastures.com, use code CarnivoreMD for 10% off your first order Eight Sleep: www.eightsleep.com/carnivoremd for exclusive Labor Day Savings through 9/11 and to get $150 off your order White Oak Pastures: www.whiteoakpastures.com, use code CarnivoreMD for 10% off your first order or Carnivore5 for 5% off subsequent orders

M. Elizabeth Younger, CRNP, PhD, Assistant Professor of Pediatrics at Johns Hopkins University School of Medicine joined us at a recent IDF forum to discuss immunoglobulin replacement therapy and the various ways it can be tweaked to fit each patient's unique needs. The information, terminology, and opinions presented in this forum do not necessarily reflect the views of IDF, its Board of Trustees, sponsors, or donors. To view the slideshow used in the presentation, click here: https://primaryimmune.org/sites/default/files/4-For%20Website-IDF%20Forum-Ig%20Therapy-FINAL-5.19.22.pdf Thank you to Takeda, CSL Behring, Grifols, and Accredo for sponsoring this event.

Dr. Rae Bacharach discusses the Neurology Today article, "Maintenance Intravenous Immunoglobulin Prevents Relapse in Adult Myelin Oligodendrocyte Glycoprotein Antibody–Associated Disease". Show references: https://journals.lww.com/neurotodayonline/Fulltext/2022/05190/Maintenance_Intravenous_Immunoglobulin_Prevents.1.aspx

"Is Intravenous Immunoglobulin Therapy for Chronic Pain Conditions an Underused Treatment Option?" by Nirguna Thalla, MD, MBA, Resident, MedStar Georgetown University, Washington, DC; Keith Newton, MD, Resident, University of Central Florida College of Medicine, Orlando, Florida; and Dalia Elmofty, MD, Associate Professor, University of Chicago Medicine, Chicago, Illinois. From ASRA Pain Medicine News, February 2022. See original article at www.asra.com/asra-news for figures and references. This material is copyrighted.   

Join 3 experts in discussion about how to best identify patients at high-risk of progression and the role of proteinuria in IgA nephropathy management. Credit available for this activity expires: 4/4/2023 Earn Credit / Learning Objectives & Disclosures: https://www.medscape.org/viewarticle/971060?src=mkm_podcast_addon_971060

Cardiac amyloidosis is an often overlooked and thus misdiagnosed form of a restrictive cardiomyopathy in which delayed diagnosis is associated with significant morbidity and mortality. Immunoglobulin light chain (AL) cardiac amyloidosis is the most deadly from of cardiac amyloidosis but significant progress has been made in treatment with the first FDA approved therapy for this condition - daratumumab. In this interview, Mathew Maurer, MD and Stephen J. Nicholls, MBBS, PhD, FACC, with Yuvraj Chowdhury, MD, discuss Pathophysiology and Therapeutic Approaches to Cardiac Amyloidosis--Focus on AL.

Ladies, are you ready to reclaim your health? Ready to see how fixing your gut health can fix your overall health? If you're ready to feel like YOU again, then this episode is a must listen to! Today, on a solo episode with me, I'm talking all about leaky gut! I'll be sharing what it is, what causes it and how to heal from it.On this podcast, I talk more on…A breakdown of what leaky gut actually isThe constant influx of things that are causing leaky gutIssues that can arise from having leaky gutHow it's a necessary component to activate autoimmune processes Why you might need a probiotic Removing the triggers that irritate your gutWhat leaky gut might look like for youThe best things you can do to heal your gutDon't forget to sign up for my free resource!The Functional Gynecologist's Guide to Balancing your Hormones: https://lcvjtpc8.pages.infusionsoft.net/Connect with me: Dr. Tabatha's Website: https://www.drtabatha.com/Dr. Tabatha's Facebook: https://www.facebook.com/DrTabathaDr. Tabatha's IG: https://www.instagram.com/thegutsygynecologist/Dr. Tabatha's YouTube: https://www.youtube.com/c/TheGutsyGynecologist@Dr_Tabatha was hacked on Instagram! Please report and UNFOLLOW that account. Please follow the real Dr. Tabatha @thegutsygynecologistWe can't let evil win!!@Dr_Tabatha was hacked on Instagram! Please report and UNFOLLOW that account. Please follow the real Dr. Tabatha @thegutsygynecologistWe can't let evil win!!

In this episode of The Milkshakes for Marleigh Podcast we profile Julian, a recently medically discharged Soldier of the Australian Army who suffers from Acute Disseminated Encepalomyelitis (ADEM) which is a type of Autoimmune Encephalitis and Myelin Oligodendrocyte Glycoprotein (MOG), which both require Intravenous Immunoglobulin Infusion IVIg, which is made from human plasma donations.  We also explore the impact that being a surviving twin has had on Julian's sense of identity and resilience. And the incredible role that dogs can play in physical, mental and  rehabilitation.  We dedicate this episode to Julian's twin brother Jeremy and recognise the special role that twins who have passed away play in the lives of the families who love them. 

This episode will explore killer cell immunoglobulin-like receptor (KIR) testing in solid organ and hematopoietic cell transplantation with experts Drs. Paula Arnold and Luis Hidalgo. We will explore recent literature on KIR utility in clinical decision-making.

Millioner af mennesker lider af autoimmune sygdomme hvor kroppen gør skade på sig selv og risikere at slå sig selv ihjel.Én type autoimmunitet er allergi. Men hvorfor bliver vi overhovedet allergiske? Hvorfor har menneskeheden udviket sig til at kunne dø af at smage på en jordnød? Måske er allergi faktisk ikke farligt for os, men i virkeligheden et gavnligt respons på en farlig omverden.Hør med når Mark fortæller om mekanismerne bag IgE responset, og når han analyserer de to førende hypoteser om hvorfor vi bliver allergiske; er det fordi vi keder os, eller er det i virkeligheden et system der fungerer præcis som det er tiltænkt?Hvis du vil være med til at optage live med os på Discord kan du støtte os på 10er og blive en af vores kernelyttere https://bit.ly/VU10er - hvis pengene er knappe kan du også bare tjekke vores Facebookgruppe ud, vi hygger max!Du kan også tjekke vores webshop: bit.ly/vushop. Vi har T-shirts, kaffekopper og tasker! Og meget mere! Der er også en hønsetrøje!Send os water hilarious science eller stil et spørgsmål på facebook, Instagram eller vudfordret@gmail.comTak til Christian Eiming for disclaimer.Tak til Barometer-Bjarke for Gak-O-meteretHusk at være dumme

Looking for nutrient-based products that are shown to support the immune system, gi system, and more? Learn about some of the most research-backed products in the industry on episode 21 of the Fredrickson Health Show 0:00 Intro 0:41 Guest Intro (Brian Kaufman) 2:25 Topic intro (Gut health) 3:22 Medical Food vs Supplement 4:00 What is medical food? 5:52 How did Brian get to this world of nutrition? 10:34 Meet Rodney 12:41 What makes gut health so important 14:04 Immunoglobulin (im-yeh-no-GLOB-yeh-len) 17:29 Immunoglobulin in oral form 20:14 Mechanism of action for oral IGG 22:29 What does Immunoglobulin bind to? 26:39 Immunoglobulin Analogy 28:39 How to know that it's not binding to the good bacteria in our body 29:51 True to life Immunoglobulin experience 31:57 Brian's health journey 35:03 AHA! Moments 37:05 Gut health to brain health connection 40:20 Immunoglobulin impact on athletes 45:24 New clinical studies to be excited for 49:34 Should everyone take SBI protect? 50:32 Immunoglobulin on kids 53:20 How / When can you take it? 53:58 The Billboard Question 55:13 What health-related tool would you bring on a deserted island? 59:48 Where to connect with Brian Kaufman Follow me! Instagram: Dr.Robert Fredrickson Instagram Business: FredricksonHealthSolutions TikTok: FredricksonHealth Facebook: FredricksonHealthSolutions Social Media Stream Page-https://beacons.page/fredricksonhealth Disclaimer: Also consult with a physician before starting a new rehab exercise, training routine, diet, or dietary supplement. This video is for educational purposes only. Always ask your doctor or physcian for personalized medical advice.

Do you struggle with chronic itchy skin and hives caused by histamine overload? Immunoglobulin might be a game-changer! My guest today is Kiran Krishnan, a Research Microbiologist who has been involved in the dietary supplement and nutrition market for the past 18 years. He comes from a University research background, having spent several years with hands-on R&D in the fields of molecular medicine and microbiology at the University of Iowa. Kiran established a Clinical Research Organization where he designed and conducted dozens of human clinical trials in human nutrition. He is also a co-founder and partner in Nu Science Trading, LLC.: a nutritional technology development and research company. In addition, Kiran is a co-founder and Chief Scientific Officer at Microbiome Labs that makes products like MegasporeBiotic + MegaIgG2000. Kiran is currently involved in 16 novel human clinical trials on probiotics and the human microbiome. Join us as we talk how immunoglobulins might be helpful for people with histamine problems. Have you tried immunoglobulins for histamine issues? Tell me about it in the comments! In this episode: How can immunoglobulins help with histamine overload? Why IgE responses can become more severe How to start Mega IgG (and other immunoglobulin products) Can you take immunoglobulins if you're immunocompromised? Common FAQs asked by readers + listeners about using Mega IgG2000 Quotes “What is the root cause that is driving this histamine overload, this high eosinophils, this itchiness, intolerance everywhere throughout the body? It all starts in the gut, obviously.” [23:11] “If you don't have a good, healthy, diverse microbiome, you've got constant leakiness in the gut, so lots of inflammation in the gut.” [26:19] Links Click HERE to try MegaIg2000 + Megasporebiotic Healthy Skin Show ep. 10 How Your Skin Health Is Tied To Your Whole-Body Health w/ Kiran Krishnan Healthy Skin Show ep. 11 The Role Of Probiotics When It Comes To Skin Health w/ Kiran Krishnan Healthy Skin Show ep. 181: Fascinating Research On Staph Infections + Probiotics (PART 1) w/ Kiran Krishnan Healthy Skin Show ep. 182: Fascinating research on Staph Infections + Probiotics (PART 2) w/ Kiran Krishnan Healthy Skin Show ep. 188: Histamine Intolerance + Skin Rashes Healthy Skin Show ep. 166: Mold + Histamine Connection w/ Dr. Jill Crista Follow Kiran Krishnan on Instagram

Have you ever heard of immunoglobulins? My guest today will explain all about them, as well as why they may be helpful for skin conditions. My guest today is Kiran Krishnan, a Research Microbiologist who has been involved in the dietary supplement and nutrition market for the past 18 years. He comes from a University research background, having spent several years with hands-on R&D in the fields of molecular medicine and microbiology at the University of Iowa. Kiran established a Clinical Research Organization where he designed and conducted dozens of human clinical trials in human nutrition. He is also a co-founder and partner in Nu Science Trading, LLC.: a nutritional technology development and research company. In addition, Kiran is a co-founder and Chief Scientific Officer at Microbiome Labs that makes products like MegasporeBiotic + MegaIgG2000. Kiran is currently involved in 16 novel human clinical trials on probiotics and the human microbiome. Join us as we talk how immunoglobulins might be helpful for skin conditions. Have you tried immunoglobulins for your skin issue? Tell me about it in the comments! In this episode: What are immunoglobulins? Different types of antibodies Connection between diarrheal issues and immunoglobulins Can IgG help with fungal overgrowth? Quotes “Immunoglobulin is a big, fancy, nerdy term for antibodies. .” [0:44] “If you have leakiness in the gut, you have things like endotoxins that leak through the lining of the gut, enter into the enteric nervous system, it can move up the vagus nerve and lodge itself in the dorsal vagal complex thereby shutting off the communication between the gut and the brain.” [14:26] Links Click HERE to try MegaIg2000 + Megasporebiotic Healthy Skin Show ep. 10 How Your Skin Health Is Tied To Your Whole-Body Health w/ Kiran Krishnan Healthy Skin Show ep. 11 The Role Of Probiotics When It Comes To Skin Health w/ Kiran Krishnan Healthy Skin Show ep. 181: Fascinating Research On Staph Infections + Probiotics (PART 1) w/ Kiran Krishnan Healthy Skin Show ep. 182: Fascinating research on Staph Infections + Probiotics (PART 2) w/ Kiran Krishnan Follow Kiran Krishnan on Instagram

This episode covers immunodeficiency caused by disorders of B cells and immunoglobulins.Written notes can be found at https://zerotofinals.com/paediatrics/immunology/igdisorders/ or in the immunology section of the Zero to Finals paediatrics book.The audio in the episode was expertly edited by Harry Watchman.

Practical Strategies in Optimising Subcutaneous Immunoglobulin Replacement Therapy

Practical Strategies in Optimising Subcutaneous Immunoglobulin Replacement Therapy

Practical Strategies in Optimising Subcutaneous Immunoglobulin Replacement Therapy

Practical Strategies in Optimising Subcutaneous Immunoglobulin Replacement Therapy

Practical Strategies in Optimising Subcutaneous Immunoglobulin Replacement Therapy

Practical Strategies in Optimising Subcutaneous Immunoglobulin Replacement Therapy

This episode covers immunoglobulin isotypes!

This episode covers immunoglobulin epitopes!

This episode is also available as a blog post: https://fierceautie.com/2020/12/04/quackery-exposed-intravenous-immunoglobulin-ivig-for-autism/ --- This episode is sponsored by · Anchor: The easiest way to make a podcast. https://anchor.fm/app

Learn about the discovery of immunodeficiencies leading to the use of pooled immunoglobulin as therapy, the struggle to use it intravenously and the eventual use in autoimmune disease. Intro :11 In this episode :15 Splitting Part 3 into two episodes :26 Part A of the history of IV Ig and the antibody :53 A recap on Cohn fractionation 2:40 The history and uses of IV Ig 4:30 What led to the recognition and research of primary immunodeficiencies 7:08 How Charles Janeway Sr. paved the way for IV Ig from intramuscular Ig 9:01 What are protein aggregates? 12:54 Understanding the various mechanisms of Ig reactions 15:49 Managing patients with low IgA and anti-IgA antibodies 18:36 How to go from treating immunodeficiency to treating autoimmunity 21:40 The modern understanding and mystery of IV Ig 25:30 Quick summary 26:05 Thanks for listening! 27:30 Disclosure: Brown reports no relevant financial disclosures. We’d love to hear from you! Send your comments/questions to Dr. Brown at rheuminationspodcast@healio.com. Follow us on Twitter @HRheuminations @AdamJBrownMD @HealioRheum References: Barandun S, et al. Vox Sang. 1962;7:157-174. Eibl MM. Immunol Allergy Clin North Am. 2008;28:737-764, viii. Furusho K, et al. Lancet. 1983;2:1359. Gallagher PE, Buckley RH. J Allergy Clin Immunol. 1982;69:120. Guo Y, et al. Front Immunol. 2018;9:1299. Imbach P, et al. Lancet. 1981;1:1228-31. Intravenous Immunoglobulin: Prevention and Treatment of Disease. NIH Consens Statement Online. 1990;8:1-23. Kustiawan I. PLoS ONE. 2018;13: e0195729. https://doi.org/10.1371/journal.pone.0195729. MRC Working Party on Hypogammaglobulinaemia. Hypogammaglobulinaemia in the United Kingdom. London: Her Majesty’s Stationery Office; 1971. Oransky I. Lancet. 2003;362:409. Sandler SG, et al. Transfus Med Rev. 1995;9:1-8.

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2020.10.02.323303v1?rss=1 Authors: Pradhan, T., Annamalai, K., Sarkar, R., Huhn, S., Hegenbart, U., Schoenland, S., Faendrich, M., Reif, B. Abstract: Systemic antibody light chains (AL) amyloidosis is characterized by deposition of amyloid fibrils derived from a particular antibody light chain. Cardiac involvement is a major risk factor for mortality. Using MAS solid-state NMR, we study the fibril structure of a recombinant light chain fragment corresponding to the fibril protein from patient FOR005, together with fibrils formed by protein sequence variants that reflect the closest germline (GL) sequence. Both analyzed fibril structures were seeded with ex-vivo amyloid fibrils purified from the explanted heart of this patient. We find that residues 11-42 and 69-102 adopt {beta}-sheet conformation in patient protein fibrils. We identify glycine-49 that is mutated with respect to the germline sequence into arginine-49 as a key residue that forms a salt bridge to aspartate-25 in the patient protein fibril structure. Fibrils from the GL protein and from the patient protein harboring the single point mutation R49G can be both heterologously seeded using patient ex-vivo fibrils. Seeded R49G fibrils show an increased heterogeneity for the C-terminal residues 80-102 which is reflected by the disappearance of all resonances of these residues. By contrast, residues 11-42 and 69-77, which are visible in the MAS solid-state NMR spectra show 13C chemical shifts that are highly similar to patient fibrils. The mutation R49G thus induces a conformational heterogeneity at the C-terminus in the fibril state, while the overall fibril topology is retained. Copy rights belong to original authors. Visit the link for more info

Dr. Emmanuel (Mike)Vasilomanolakis, MD is a Cardiology Specialist in Long Beach, CA and has over 42 years of experience in the medical field. ... COVID-19 UPDATE The latest summary information regarding the prevention and treatment of COVID-19 infections is as follows. For prevention vitamin C is an immune booster. Take 500 to 2000 mg daily. Vitamin D has been suggested to boost immunity and lessen the bradykinin storm that happens when you get really sick. Take 1000 to 4000 units per day. Perhaps the most important is zinc. Not sure what the optimum dose is because at higher doses there is loss of taste, etc. Probably 40 to 100 mg per day should suffice. For additional potential prophylaxis you can consider these agents. Melatonin 2-6 mg at night (remember this is also a sleeping pill and can make you sleepy) and Quercetin 250-500 mg daily. This is based on a poll of doctors from a conference. I am not saying you need to get these but these are some additional professional ideas. If you develop the infection in order to minimize the chance of blood clot formation take aspirin 81-325 mg per day at the time you become sick or think you are coming down with something. If you are hospitalized they will start you on more potent blood thinners (anticoagulants). There has been some controversy regarding the use of Pepcid (Famotidine) which is an antacid medication. It is thought that perhaps at high doses its anatomic spike protein similarity with the COVID-19 may compete with the virus for H2 receptor sites and as such may lessen the severity of the illness. There is no need to take this prophylactically. However, like Aspirin you can start it if you think you are getting sick. Recommended dose is 40 mg daily tho in the hospital they give a much higher dose either by pill or IV....however from my experience many doctors are not using this. There are several other treatments proposed for once you become sick including Fenofibrate which is an anti-triglyceride agent but more interestingly Ivermectin which is an anti-scabies medication. There is a researcher from Australia that convincingly advocates this in particular with the antibiotic Doxycycline and some additional Zinc. They have shown the virus clears more rapidly from your system. Should you become hospitalized there are several inflammatory blood markers they monitor such as CRP and sedimentation rate. If these become elevated you will be started on steroid therapy. Steroid inhalers early on in the infection have been advocated and may help. You will also be automatically started on anticoagulation therapy to prevent blood clots. There is the intravenous medication Remdesivir which when started early seems to lessen the severity and quickens recovery. It does not work the same on all patients but it may be the best treatment we have for now. The patient is given oxygen and every effort is made to avoid being placed on mechanical ventilation including rotating the patient face up and face down repeatedly to let all parts of the lungs get full ventilation and expansion . If there is any suggestion of infection antibiotics are started. Convalescent plasma from people who have recovered is also often used/transfused. The monitoring and treatment regimens have become more standardized and the results improving. A couple months ago the mortality rate for those with known active PCR positive Covid-19 infection was 6%. Now it is down to 3%. This again is based on known cases. There are many more who have had no or minimal symptoms who have had the disease but never became sick enough to get a proof positive PCR exam. They are found to have had the infection by a blood test Immunoglobulin assay which detects antibodies. If your ( IgG and IgM) test is positive it suggests you have had the infection and now have immunity The duration of such immunity however is uncertain. It can be 4 or more months and yes you can get reinfected but it is rare and the likelihood is it will be a less severe infection. When this pandemic is done and over with the mortality rate may well dip under 1% when taking into account all with antibody positivity. Tho the news is better don’t drop your guard. We are in our 7th month of isolation. We are almost there. Give it 2-3 more months and there will be mass vaccination and we will be in a much safer position. Pardon the brevity of this assessment. I welcome my colleagues and all knowledgeable individuals to add or comment. Best wishes to you, Dr. Mike Vasilomanolakis

We are back for the 4th and final season of the SIBO Made Simple podcast—and man, what a different world we’re returning to. I could have never predicted when I recorded my last episode on February 20th that all anyone would be thinking about in the health space was viruses. But here we are. To satisfy all your burning questions about Covid-19 and further educate ourselves on how the immune system interacts with our gut, I’ve got Dr. Susan Blum returning to the show to discuss all things antibodies. As someone with an autoimmune disease (and the host of this here podcast), I thought I was pretty hip to this line of immune defense, but all the recent discussion of viral antibodies has revealed that there are some major gaps in my knowledge on how these immunoglobulins are produced, what functions they serve, and why they sometimes hurt rather than help. Dr. Blum is the author of the iconic book The Immune System Recovery Plan and more recently, Healing Arthritis. She is also our featured guest on episode 8 of the podcast discussing leaky gut and the SIBO-autoimmune connection. It’s a great place to start if you’re new to how the immune system works. A quick taste of what we’ll cover: The difference between T-cells and B-cells How long different types of antibodies stick around for Antibody testing and why there have been problems with Covid-19 results Other ways our body develops defenses to viruses besides antibodies that makes a second infection less likely How antibodies to foods form and what IgG food sensitivity testing tells you about your long-term tolerance to certain ingredients Whether SIBO or other forms of dysbiosis make you more immunocompromised or susceptible to viruses Immunoglobulin supplements and other ways to fortify your immune system And so much more… Resources, mentions and notes: Dr. Blum’s website Dr. Blum’s books: The Immune System Recovery Plan and Healing Arthritis Dr. Blum’s new course Episode 8: The SIBO-Autoimmune Connection with Dr. Blum SBI Protect immunoglobulins; IgG protect LoveBug Probiotics Immunity Formula Pre-Order the SIBO Made Simple book for immediate access to recipes! Join the SIBO Made Simple Facebook Community Page Subscribe to the newsletter (and receive a free low FODMAP cookbook) This episode is brought to you by 4 Weeks to Wellness, my online program for finding the sweet spot between health and hedonism. Need 4-weeks worth of easy low FODMAP recipes with a step-by-step meal plan laid out for you? You’ll receive a 20-recipe cookbook, complete with shopping lists, elimination diet guidance and reintroduction worksheets, in addition to the many other program resources like my symptom and activity tracker, lymphatic massage videos, doable desk stretches, and so much more. The 300+ people who have joined the tribe have reported clearer skin, calmer tummies, less bloat, tamer food cravings, and a general ease over their newfound habits. To find out more details about when the next session starts, click here. Use the code SIBOMADESIMPLE at checkout for $40 off! Disclaimer: The information in this podcast does not provide medical advice, professional diagnosis, opinion, or treatment. The information discussed is for educational purposes only and is not a substitute for medical or professional care.

FA 2020 - p105 Immunoglobulin isotypes, antigen type and memory

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2020.08.19.257501v1?rss=1 Authors: Jackson, M. A., Pearson, C., Ilott, N. E., Huus, K. E., Hegazy, A. N., Webber, J., Finlay, B. B., Macpherson, A. J., Powrie, F., Lam, L. H. Abstract: Background: Identifying which taxa are targeted by immunoglobulins can uncover important host-microbe interactions. Immunoglobulin binding of commensal taxa can be assayed by sorting bound bacteria from samples and using amplicon sequencing to determine their taxonomy, a technique most widely applied to study Immunoglobulin A (IgA-Seq). Previous experiments have scored taxon binding in IgA-Seq datasets by comparing abundances in the IgA bound and unbound sorted fractions. However, as these are relative abundances, such scores are influenced by the levels of the other taxa present and represent an abstract combination of these effects. Diversity in the practical approaches of prior studies also warrants benchmarking of the individual stages involved. Here, we provide a detailed description of the design strategy for an optimised IgA-Seq protocol. Combined with a novel scoring method for IgA-Seq datasets that accounts for the aforementioned effects, this platform enables accurate identification and quantification of commensal gut microbiota targeted by host immunoglobulins. Results: Using germ-free and Rag1-/- mice as negative controls, and a strain-specific IgA antibody as a positive control, we determine optimal reagents and fluorescence activated cell sorting (FACS) parameters for IgA-Seq. Using simulated IgA-Seq data, we show that existing IgA-Seq scoring methods are influenced by pre-sort relative abundances. This has consequences for the interpretation of case-control studies where there are inherent differences in microbiota composition between groups. We show that these effects can be addressed using a novel scoring approach based on posterior probabilities. Finally, we demonstrate the utility of both the IgA-Seq protocol and probability-based scores by examining both novel and published data from in vivo disease models. Conclusions: We provide a detailed IgA-Seq protocol to accurately isolate IgA-bound taxa from intestinal samples. Using simulated and experimental data, we demonstrate novel probability-based scores that adjust for the compositional nature of relative abundance data to accurately quantify taxon-level IgA binding. All scoring approaches are made available in the IgAScores R package. These methods should improve the generation and interpretation of IgA-Seq datasets and could be applied to study other immunoglobulins and sample types. Copy rights belong to original authors. Visit the link for more info

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2020.08.07.242347v1?rss=1 Authors: Eguchi, R. R., Anand, N., Choe, C. A., Huang, P.-S. Abstract: While deep learning models have seen increasing applications in protein science, few have been implemented for protein backbone generation -- an important task in structure-based problems such as active site and interface design. We present a new approach to building class-specific backbones, using a variational auto-encoder to directly generate the 3D coordinates of immunoglobulins. Our model is torsion- and distance-aware, learns a high-resolution embedding of the dataset, and generates novel, high-quality structures compatible with existing design tools. We show that the Ig-VAE can be used to create a computational model of a SARS-CoV2-RBD binder via latent space sampling. We further demonstrate that the model's generative prior is a powerful tool for guiding computational protein design, motivating a new paradigm under which backbone design is solved as constrained optimization problem in the latent space of a generative model. Copy rights belong to original authors. Visit the link for more info

The second edition of The Carnivore Code (new cover and index!) is available for pre-order now!  www.Thecarnivorecodebook.com release date is August 4th 2020 in ebook, print, and audiobook formats.    “Dr. Al” Danenberg is a periodontist and was in private practice for 44 years. He incorporated ancestral nutrition & lifestyle with his leading-edge laser protocol to treat periodontal disease. In September 2018, he retired from the practice of treating individual patients. However, he still consults with patients by phone, Skype, or Zoom regarding nutrition, lifestyle, oral and overall health, and the importance of a healthy gut. Dr. Al received advanced training in evolutionary nutrition from the Kripalu Center for Yoga and Health as well as The Center for Mind-Body Medicine.    In June 2014, he receivedhis Certified Functional Medicine Practitioner (CFMP) designation as well as hiscertification as a Certified Primal Health Coach. In 2015, Dr. Danenberg was appointed tothe faculty of the College of Integrative Medicine and created the college’s integrativeperiodontal teaching module. Then, in April 2017, he earned the designation of “ADAPTTrained Health Professional” from Kresser Institute. In July 2017, Elektra Press released Dr.Danenberg’s book, “Crazy-Good Living”, which is based on ancestral nutrition and lifestyle.   On April 14, 2020, Dr. Danenberg was appointed the Chair of the Periodontal Committee for the IABDM. He also created the Certification Program for Biological Nutritional Dental Professionals offered by the IABDM. Dr. Danenberg can be contacted from his website – https://drdanenberg.com/contact/   Time Stamps: 8:04 Start of Podcast 8:29 Al’s Story 25:49 Al’s current diet  32:39 Processed carbohydrates. 33:34 Pitfalls of Western medicine ideology. 39:24 Periodontal health and diet.  48:13 Does everything start in the mouth?  53:46 Plaque and dental health. 56:40 Over Sanitization. 57:54 Is mouthwash good for you?  1:01:04 Oral probiotics.  1:02:54 Ancestral microbiome. 1:05:04 What other than food can damage our microbiome? 1:06:57 Happiness of indigenous people. 1:09:14 Story of one of Al’s patients. 1:16:39 Cheat days. 1:18:59 Xylitol gum and fluoride. 1:22:28 Colloidal Silver 1:24:59 How to clean Your teeth  1:26:24 Honey and dental health. 1:35:14 Multiple myeloma immunology/Al’s regimen.  1:47:04 Benefits of eating red meat with a cancer diagnosis.  1:56:24 Spore based probiotics/Al’s regimen continued.  2:04:04 Why Paul dislikes plant based compounds. 2:07:39 How Al’s health is doing today. 2:11:34 Why Coronavirus is affecting Americans so severely. 2:13:00 Discussing cancer. 2:25:04 The most radical thing Al has done recently. References:Honey and Oral Healthhttps://pubmed.ncbi.nlm.nih.gov/25057231/ https://pubmed.ncbi.nlm.nih.gov/27050177/ https://pubmed.ncbi.nlm.nih.gov/21187354/#:~:text=Ethiopian%20multiflora%20honey%20has%20antifungal,honey%20to%20treat%20oral%20lesions. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5855267/ https://drdanenberg.com/manuka-honey-mouth-health/ Multiple Myelomahttps://en.wikipedia.org/wiki/Immunoglobulin_light_chain https://en.wikipedia.org/wiki/Daratumumab Paleo Medicina Case Studieshttps://www.paleomedicina.com/en/glioblastoma_paleolithic_ketogenic_diet http://pubs.sciepub.com/jcrt/6/1/1/ https://www.researchgate.net/publication/308222208_Halted_Progression_of_Soft_Palate_Cancer_in_a_Patient_Treated_with_the_Paleolithic_Ketogenic_Diet_Alone_A_20-months_Follow-up https://www.researchgate.net/publication/319266162_Treatment_of_Rectal_Cancer_with_the_Paleolithic_Ketogenic_Diet_A_24-months_Follow-up Gut Microbiomehttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6747470/ https://microbiomejournal.biomedcentral.com/articles/10.1186/s40168-019-0704-8/tables/2 Certified Biological Nutritional Dental Professional Coursehttps://iabdm.org/product/certified-biological-nutritional-dental-professional-course/ CDC Obesity Data https://www.cdc.gov/obesity/index.html Cancer https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4303887/   Nutrisense (Continuous Glucose Monitor- CGM): www.Nutrisense.io, use the code CarnivoreMD for 20$ off.    BluBlox: www.blublox.com use the code CarnivoreMD for 15% off your order   White Oak Pastures: Use the code CARNIVOREMD at www.whiteoakpastures.com for 10% off your first order!   JOOVV: www.joovv.com/paul   To subscribe to my newsletter visit: carnivoremd.com   My contact information:   Book: www.thecarnivorecodebook.com   PATREON: https://www.patreon.com/paulsaladinomd   SOCIAL MEDIA  Instagram: @carnivoremd Website: carnivoremd.com Twitter:@carnivoremd  Facebook: Paul Saladino MD email: drpaul@carnivoremd.com

ವೈರಾಣು ಎಂಬುದು ಒಂದು ಜೀವಿಯ ಅಥವಾ ನಿರ್ಜೀವಿಯಾ? ಈ ಕರೋನ ವೈರಸ್ಸಿನ ಜೀವಶಾಸ್ತ್ರ ಏನು? ಪ್ರಪಂಚದ ಎಲ್ಲೆಡೆಯೂ ಹರಡಿರುವ ಈ ಸಾಂಕ್ರಾಮಿಕ ವೈರಾಣುಗಳಿಗೆ ಚಿಕಿತ್ಸೆ ಹೇಗೆ ನೀಡಲಾಗುತ್ತಿದೆ? ಈ ವೈರಾಣುವಿಗೆ ಲಸಿಕೆ ಬರಬೇಕೆಂದರೆ ಎಷ್ಟು ದಿನಗಳಾಗಬಹುದು?ಇವೆಲ್ಲರ ಬಗ್ಗೆ ಚರ್ಚೆ ಮಾಡಲು ನಮ್ಮ ೬೪ನೇ ಸಂಚಿಕೆಯಲ್ಲಿ ಡಾ| ಶಶಿ ತೂಟುಪಲ್ಲಿ ಮತ್ತು ಪವನ್ ಶ್ರೀನಾಥ್ ಅವರು ಗಣೇಶ್ ಚಕ್ರವರ್ತಿಯವರ ಜೊತೆ ಕರೋನ ವೈರಸ್ಸಿನ ಚಿಕಿತ್ಸೆ, ಲಸಿಕೆ ಪ್ರಯತ್ನಗಳ ಬಗ್ಗೆ ಮತ್ತು ಇದರ ಜೀವಶಾತ್ರದ ಬಗ್ಗೆ ಚರ್ಚೆ ಮಾಡುತ್ತಾರೆ. ಈ ಸಂಚಿಕೆಯು ಮೂರು ಭಾಗಗಳಲ್ಲಿ ವಿಂಗಡಿಸಲಾಗಿದೆ. ಪ್ರತಿಯೊಂದನ್ನು ಪ್ರತ್ಯೇಕವಾಗಿ ಕೇಳಬಹುದು.ಶಶಿ ತೂಟುಪಲ್ಲಿ ಅವರು ಏನ್.ಸಿ.ಬಿ.ಎಸ್ ಸಂಸ್ಥೆಯಲ್ಲಿ ಪ್ರೊಫೆಸರ್ ಆಗಿದ್ದರೆ. ಇವರ ಸಂಶೋಧನೆಯು ಜೀವಿಕ ವ್ಯವಸ್ಥೆಗಳ ಮೇಲೆ ಹಾಗೂ ಅವುಗಳ ಸ್ವಯಂ ವ್ಯವಸ್ಥೆಗಳ ಮೇಲೆ ಆಗಿದೆ. ಅವರ ಲ್ಯಾಬ್ ವೆಬಸೈಟ್: https://www.thutupallilab.com/ಪವನ್ ಶ್ರೀನಾಥ್ ಅವರು ಕರೋನ ವೈರಸ್ ಸುತ್ತ ಅನೇಕ ಸಂಚಿಕೆಗಳನ್ನು ನಾನಾ ಭಾಷೆಯಲ್ಲಿ ಕೂಡ ಮಾಡಿದ್ದಾರೆ. ಇವೆಲ್ಲವನ್ನೂ ಕೇಳಲು ಭೇಟಿ ನೀಡಿ: https://tinyurl.com/covid19-pavanIs a virus living, or non-living? What is the basic biology of the Coronavirus or SARS-COV-2? How are India and the world testing and diagnosing people with this infection? How should we think of immunity against this disease? How far away are we from developing vaccines and drugs to cure or manage COVID-19, and what is the development process like?Shashi Thutupalli and Pavan Srinath join host Ganesh Chakravarthi (@craynonymous) on Episode 64 of the Thale-Harate Kannada Podcast to discuss Coronavirus biology, diagnostics, immunity, vaccine and drug development.This longer than usual episode is in three parts: which can each be listened to independently.Shashi Thutupalli (@Stpalli) is a Professor at the National Centre for Biological Sciences, NCBS, Bengaluru. He also has a joint appointment at the International Centre for Theoretical Sciences (ICTS) and is a part of the Simons Centre for the Study of Living Machines at NCBS. His research combines theory and experiments to investigate the origins and self-organization of living systems. Lab website: https://www.thutupallilab.com/Pavan Srinath (@zeusisdead), apart from hosting Thale-Harate, studied biotechnology and biophysics, before working in public policy. Apart from working on health and life science policy research over the last few years, Pavan has also recorded a dozen episodes on various issues of the COVID-19 pandemic with expert vaccinologists, economists, disease modelers, and others, across multiple podcasts. Links to all here: https://tinyurl.com/covid19-pavanಫಾಲೋ ಮಾಡಿ. Follow the Thalé-Haraté Kannada Podcast @haratepod.Facebook: https://facebook.com/HaratePod/Twitter: https://twitter.com/HaratePod/Instagram: https://instagram.com/haratepod/ಈಮೇಲ್ ಕಳಿಸಿ, send us an email at haratepod@gmail.com and tell us what you think of the show.Subscribe & listen to the podcast on iTunes, Google Podcasts, Castbox, AudioBoom, YouTube, Souncloud, Saavn, Spotify or any other podcast app. We are there everywhere. ಬನ್ನಿ ಕೇಳಿ!

--{ "C'mon, Everybody Sing...We'll meet Again, Don't Know Where, Don't Know When, If We're Allowed Out of Our Homes Some Sunny Day.- Complete" © Alan Watt }-- Chip, Electronic Tag; Vaccinations Up to Date or Alarms Go Off - Behavioural Insights Teams (BIT) - Internet, ARPA, DARPA - Wartime Scenarios to Push Through Agenda - 2007-08 Crash - Devalued Canadian Dollar - Martial Law, Emergency Powers - Plundered by Big Banks Two or Three Times a Century - We're Not Allowed Peace - Data Collection - Edward Bernays Uncle was Sigmund Freud; Propaganda WWI; American Fruit Company, Overthrowing a Government - UK, US Income Tax Systems; Warburg - Think Tanks Plan Futures - Corporations, An Organism Alters the Terrain for its Own Survival - Organized Gang Runs the World - Lunar Society, Rothschild - Brotherhoods, Masonry, CFR, RIIA, Milner Group; League of Nations, United Nations, IMF, World Bank, BIS - Those Who Shape Our Culture - Georgia Guidestones - Star-Making Machinery; Scientists Made Stars - Bill Gates - Philanthropists All on Board with the Same Program - Public-Private Partnerships - Malthus - Bertrand Russell, Culling Off the Poor and Infirm; Diet, Injections and Injunctions - Hitler's Eugenics Ideas from Britain and U.S.; Cold Spring Harbor - H.G. Wells - Youth Radicalized by Education - Myth - Time it Took to Organize the American Revolution - Benjamin Franklin, Candlestick Makers, The Light Bringers - H.G. Wells, Anticipations; Values of the Scientific Priesthood; Life and Death; Efficient - The Slaughter Done by Tyrants - Eugenics - Given Authorized Hosts to Follow - Global Lockdown - 9/11 Took so Many Rights Away - Pornography - We're in 9/11 Part Two - Brandon Turbeville Article about the COVID-19 Lockdown - Gordon Brown calls for World Government - Quarantine isn't Rocket Science - Private Banks Set Up by the World Controllers - What I Live On is Below the Poverty Level - Gordon Brown, Tax to Give to IMF to Give to Those Countries Struggling to Cope with COVID-19 Impact - H.G. Wells, Those Who Live on Opiates; Aldous Huxley, Soma - Liquor Stores Considered Essential Businesses During this Lockdown and Business Closure - Horror Stories, You Identify and Can't Look Away because You Might Die - The Vulnerable who have a Hard Time Now Getting Essential Supplies - My Hospital Visit in 2016 - Couriers don't Deliver to My Area - Stories about Celebrities getting COVID-19 - China Working on Treatment Using Antibodies, Immunoglobulin; Ebola - Limiting Access to Antibiotics - World Health Organization; U.N. used to have a Department of Population Control - Quinine and Antibiotic - If You Go to Hospital, Make Sure You have an Advocate - Canada and U.S. States that Consider Marijuana Dispensaries an Essential Business - B.C. COVID-19 Spokesperson Given Star Status with a Fan Club - Paul Craig Roberts, A Brady Bond Solution for U.S. Debt - Nearly Half of Canadians can't Afford to Miss Work During this Time - Spike Milligan; "We" are Now at War - We're All in It Together; For the Greater Good - Facts don't Matter when it Comes to Terrifying the Public to Get Them to Comply - "Snowbirds" Bring COVID-19 Back to Canada from Florida and Other Warm Climates - Event 201, Johns Hopkins University, Bill Gates - Phone Apps to Track You Everywhere You Go to See if You and Your Cluster of Friends are Infected; Echelon Programs Monitor for Certain Words and Phrases - British Television Miniseries, The Last Enemy (2008), Based on Technology that was in Use in Britain Even Then; Real-Time Monitoring, Massive Migration, Vaccines that would Track the Immigrants; Ethnic-Specific Diseases - B. Gates, Agenda ID2020, Chips and Tattoos; When I Think of Event 201, I think of James Bond Movies, Villains, Billionaires; In James Bond movie, "Tomorrow Never Dies", the Villain who Wrote the News Story and Then Made it Happen, used Slogan "Tomorrow's News Today" - Red Cross Requesting Blood but Not Testing for COVID-19; Antibodies from Folk Who've Recovered - The Ray Bradbury Theater, "The Pedestrian" from 1989, People have Been Raised, have Spent Their Whole Lives in Lockdown, a Permanent Curfew, Picked Up by a Robotic Helicopter with No Pilot - Movies, Soylent Green, Logan's Run - Orwell, Incredible Monitoring of Us All, Martial Law Techniques, Politicians Giving Us Statistics in Wartime Fashion - Please Remember to Visit www.cuttingthroughthematrix.com to Donate - One Million Flee Paris Before the Lockdown - Scientist Who Predicted Armageddon Just Walked Back Apocalyptic Predictions - Half Those Getting Put Down as Positive, haven't Been Tested; Presumptive - Old Books written after Spanish Flu of 1918, Found that the Chinese and Italians and the People from Area Now Called Iran were Hard Hit by the Spanish Flu - Chinese Immigrants Working in the Textile Business in Northern Italy - Peter Hitchens; Professor Sucharit Bhakdu - Malthus - Cytokine Storm - Antibiotics - Invisible Ink Could Reveal whether Children Have Been Vaccinated - Bill Gates, MIT Develop New ‘Tattoo ID' to Check For Vaccinations - Quantum Dot Tattoo - Paul Craig Roberts, Coronavirus Profiteers Are Worse Than War Profiteers - Think for Yourselves - Series, The Last Enemy, Know in Real Time what the Public is Thinking - Limiting Energy Consumption; Technocracy - Keep Your Heads - Don't Go Crazy - Public Relations Used to Be Called Propaganda - Don't Be Drinking and Drugging Yourselves to Death - Millions, Even Billions Being Given Out by Governments that Will Never Be Accounted For - Help Each Other Out - Listen to Some Uplifting Music. *Title and Dialogue Copyrighted Alan Watt - Mar. 29, 2020 (Exempting Music and Literary Quotes)

Padraig French joins Emma-Louise Coffey to measure the welfare of calves on Irish Dairy Farms. Padraig explains that calf mortality at birth and at one month old is a key metric to measure calf welfare. Comparing the performance in Ireland over the last 10 years, calf mortality has fallen on farms, despite an additional 300,000 calves being born annually.  Mortality at birth declined from 1.95% to 1.30% and mortality at 28 days declined from 3.71% to 3.20% comparing 2011 with 2019 figures.  Additionally, Padraig explained that the births were equal across males and female calves. Total calf in the 1st month of life at 4.5% in Ireland is half that of our international counterparts. Furthermore, he briefly reviews a trial carried out by researchers John Barry and Emer Kennedy. They measured the level of Immunoglobulin levels in the blood of calves which indicates whether a calf received adequate colostrum. Padraig explains that both male and female calves received adequate colostrum. Padraig also encourages farmers to take a look at their facilities and available labour to ensure dairy farmers continue to maintain a high calf welfare status within their own herd. For more episodes and information from the Dairy Edge podcast go to: https://www.teagasc.ie/animals/dairy/the-dairy-edge-podcast/ 

There is a global shortage of immunoglobulin therapy. This is a blood product that some patients rely on to stay alive. Speak to 2 doctors, myself and Dr. Matt Bell from the USA speak about this and how it affects our ability to treat patients.

There is a whole world of things out there that want us dead – we are talking microscopic invaders that want to get inside our bodies and kill us. Lucky for us we have a secret weapon to keep us alive…ANTIBODIES In this episode we are taking a deep dive and looking at these teeny tiny antibodies and asking….why aren’t we dead?  To find out we met up with the Head of the Department for Statistics at the University of Oxford, Charlotte Deane…

Proceedings from the First Annual Miami General Medical Oncology Symposium — Module 4: Chronic Lymphocytic Leukemia (CLL) — Featuring moderated presentations of important clinical research and ongoing clinical trials by Drs Matthew S Davids and Nitin Jain. Moderated by Dr Neil Love. Presentation of General Medical Oncologist Practice Survey Results (00:00) Newly Diagnosed CLL — Dr Davids (00:58) Management of Relapsed/Refractory CLL and Investigational Approaches — Dr Jain (23:31) Boards Mini-Review: Prevention and Management of Infections (Antimicrobials, Vaccination, Immunoglobulin) (44:07) Boards Mini-Review: Diagnosis and Treatment of Richter's Transformation (49:02) Select publications

The Immune team turns to chickens, which have been important in understanding the immune system, to show that infection of mature B cells is not required for the pathogenesis of Marek's disease herpesvirus. Hosts: Vincent Racaniello, Stephanie Langel, and Cynthia Leifer Subscribe (free): iTunes, Google Podcasts. RSS, email Become a patron of Immune! Links for this episode Role of B cells in Marek's disease pathogenesis (PNAS) In ovo vaccination (YouTube) First chickens lacking B cells (PNAS) Letters read on Immune 16 Time stamps by Jolene. Thanks! Weekly Science Picks Steph - 7 Days Out Cindy - Watson PBS documentary and Cold Spring Harbor Laboratory response Vincent - Dads can donate mitochondrial DNA and Chicken puns

Dr. Speelziek interviews Dr. Kurt Kimpinski and Dr. Luciano Sposato on their article, Subcutaneous versus intravenous immunoglobulin for chronic autoimmune neuropathies. A meta-analysis. High-dose intravenous immunoglobulin (IVIg) is an evidence-based treatment for multifocal motor neuropathy (MMN) and chronic inflammatory demyelinating polyneuropathy (CIDP). Recently, subcutaneous immunoglobulin (SC-Ig) has received increasing attention. The authors performed a meta-analysis of reports of efficacy and safety of SC-Ig versus IVIg for inflammatory demyelinating polyneuropathies.The efficacy of SC-Ig is similar to IVIg for CIDP and MMN and has a significant safety profile. Muscle Nerve 55: 802-809, 2017.

Dr. Speelziek interviews Dr. Kurt Kimpinski and Dr. Luciano Sposato on their article, Subcutaneous versus intravenous immunoglobulin for chronic autoimmune neuropathies. A meta-analysis. High-dose intravenous immunoglobulin (IVIg) is an evidence-based treatment for multifocal motor neuropathy (MMN) and chronic inflammatory demyelinating polyneuropathy (CIDP). Recently, subcutaneous immunoglobulin (SC-Ig) has received increasing attention. The authors performed a meta-analysis of reports of efficacy and safety of SC-Ig versus IVIg for inflammatory demyelinating polyneuropathies.The efficacy of SC-Ig is similar to IVIg for CIDP and MMN and has a significant safety profile. Muscle Nerve 55: 802-809, 2017.

Dr. Speelziek interviews Dr. Kurt Kimpinski and Dr. Luciano Sposato on their article, Subcutaneous versus intravenous immunoglobulin for chronic autoimmune neuropathies. A meta-analysis. High-dose intravenous immunoglobulin (IVIg) is an evidence-based treatment for multifocal motor neuropathy (MMN) and chronic inflammatory demyelinating polyneuropathy (CIDP). Recently, subcutaneous immunoglobulin (SC-Ig) has received increasing attention. The authors performed a meta-analysis of reports of efficacy and safety of SC-Ig versus IVIg for inflammatory demyelinating polyneuropathies.The efficacy of SC-Ig is similar to IVIg for CIDP and MMN and has a significant safety profile. Muscle Nerve 55: 802-809, 2017.

Can IViG be used as a longer term maintenance therapy for CIDP patients? JNNP Associate Editor, Professor Satoshi Kuwabara (Chiba University Hospital, Japan) joins us for this podcast, discussing recent trial results and their implications for patient care. Read the full paper here: http://jnnp.bmj.com/content/88/10/832

Do patients with 1st trimester bleeding need to get anti-D immunoglobulin if they're Rh negative? We dive into the topic this week. https://media.blubrry.com/coreem/content.blubrry.com/coreem/Podcast_86_0_Final_Cut.m4a Download Leave a Comment Tags: Early Pregnancy, Obstetrics, RhoGam, Vaginal Bleeding Show Notes Take Home Points An Rh negative woman can become alloimmunized to Rh antigen if exposed to blood from an Rh positive fetus. Theoretically, this alloimmunization can occur even in early pregnancy While anti-D immune globulin has clearly been shown to be beneficial in preventing alloimmunization in 2nd and 3rd trimester pregnancy, there is no evidence supporting use specifically in the 1st trimester Despite the absence of evidence, RhoGam administration has become routine in many places. At this time, it's advisable to follow local practice patterns regarding which patients should be given RhoGam. References ACOG Practice Bulletin. Prevention of Rh D Alloimmunization. Int J Gynaecol Obstet 1999; 66(1): 63-70. PMID: 10458556 Recommendations reaffirmed in 2016 Hahn SA et al. Clinical Policy: Critical Issues in the Initial Ealuation and Management of Patients Presenting to the Emergency Department in E...

Do patients with 1st trimester bleeding need to get anti-D immunoglobulin if they're Rh negative? We dive into the topic this week. https://media.blubrry.com/coreem/content.blubrry.com/coreem/Podcast_86_0_Final_Cut.m4a Download Leave a Comment Tags: Early Pregnancy, Obstetrics, RhoGam, Vaginal Bleeding Show Notes Take Home Points An Rh negative woman can become alloimmunized to Rh antigen if exposed to blood from an Rh positive fetus. Theoretically, this alloimmunization can occur even in early pregnancy While anti-D immune globulin has clearly been shown to be beneficial in preventing alloimmunization in 2nd and 3rd trimester pregnancy, there is no evidence supporting use specifically in the 1st trimester Despite the absence of evidence, RhoGam administration has become routine in many places. At this time, it’s advisable to follow local practice patterns regarding which patients should be given RhoGam. References ACOG Practice Bulletin. Prevention of Rh D Alloimmunization. Int J Gynaecol Obstet 1999; 66(1): 63-70. PMID: 10458556 Recommendations reaffirmed in 2016 Hahn SA et al. Clinical Policy: Critical Issues in the Initial Ealuation and Management of Patients Presenting to the Emergency Department in Early Pregnancy....

Hosts: Vincent Racaniello, Alan Dove, and Rich Condit The sternutating TWiVers discuss preventing infection of cells and animals by a soluble CD4-CCR5 molecule that binds to HIV-1 virus particles. Links for this episode Dif-tor heh smusma (NY Times) Immunization preterm premature infants (Thanks, Johnye!) HIV and SHIV protection conferred by eCD4-Ig (Nature) Tyrosine sulfation (Wikipedia) GBV-C and Ebolavirus infection (J Virol) Letters read on TWiV 326 Weekly Science Picks Alan - Toms River by Dan FaginRich - Spin by Robert CharlesVincent - FCC votes for net neutrality (ArsTechnica) Listener Pick of the Week Neal - Why doesn't everyone get the flu vaccine?Fernando - Zombie Jonas Salk hunts idiotsNeva - Physicists in biology Send your virology questions and comments (email or mp3 file) to twiv@twiv.tv

The recent addition of immunoglobulin (Ig)G4-associated cholangitis (IAC), also called IgG4-related sclerosing cholangitis (IRSC), to the spectrum of chronic cholangiopathies has created the clinical need for reliable methods to discriminate between IAC and the more common cholestatic entities, primary (PSC) and secondary sclerosing cholangitis. The current American Association for the Study of Liver Diseases practice guidelines for PSC advise on the measurement of specific Ig (sIg)G4 in PSC patients, but interpretation of elevated sIgG4 levels remains unclear. We aimed to provide an algorithm to distinguish IAC from PSC using sIgG analyses. We measured total IgG and IgG subclasses in serum samples of IAC (n = 73) and PSC (n = 310) patients, as well as in serum samples of disease controls (primary biliary cirrhosis; n = 22). sIgG4 levels were elevated above the upper limit of normal (ULN = >1.4 g/L) in 45 PSC patients (15%; 95% confidence interval [CI]: 11-19). The highest specificity and positive predictive value (PPV; 100%) for IAC were reached when applying the 4× ULN (sIgG4 > 5.6 g/L) cutoff with a sensitivity of 42% (95% CI: 31-55). However, in patients with a sIgG4 between 1× and 2× ULN (n = 38/45), the PPV of sIgG4 for IAC was only 28%. In this subgroup, the sIgG4/sIgG1 ratio cutoff of 0.24 yielded a sensitivity of 80% (95% CI: 51-95), a specificity of 74% (95% CI: 57-86), a PPV of 55% (95% CI: 33-75), and a negative predictive value of 90% (95% CI: 73-97). Conclusion: Elevated sIgG4 (>1.4 g/L) occurred in 15% of patients with PSC. In patients with a sIgG4 >1.4 and

Thu, 8 May 2014 12:00:00 +0100 https://edoc.ub.uni-muenchen.de/16970/ https://edoc.ub.uni-muenchen.de/16970/2/Eichner_Ruth.pdf Eichner, Ruth

May 07, 2012 In this interesting episode, host Dr. Tim Cripe (Nationwide Children's) and co-hosts Dr. Lionel Chow (Cincinnati Children's), Dr. Andy Kolb (AI DuPont), and Donna Ludwinski (Solving Kids' Cancer) quiz Dr Paul Sondel and Dr. Ken DeSantes (both from University of Wisconsin - Madison) on NK cells and the implications of KIR/KIR-ligand mismatch (killer immunoglobulin-like receptor) with regard to immunotherapy treatment of neuroblastoma. References: Delgado DC, Hank JA, Kolesar J, Lorentzen D, et al. Genotypes of NK cell KIR receptors, their ligands, and Fcγ receptors in the response of neuroblastoma patients to Hu14.18-IL2 immunotherapy. Cancer Res. 2010 Dec 1;70(23):9554-61. Epub 2010 Oct 8. http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2999644/ . Venstrom JM, Zheng J, Noor N, Danis KE, et al. KIR and HLA genotypes are associated with disease progression and survival following autologous hematopoietic stem cell transplantation for high-risk neuroblastoma. Clin Cancer Res. 2009 Dec 1;15(23):7330-4. Epub 2009 Nov 24. http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2788079/ . Alderson KL, Sondel PM. Clinical cancer therapy by NK cells via antibody-dependent cell-mediated cytotoxicity. J Biomed Biotechnol. 2011;2011:379123. Epub 2011 May 24. http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3110303/

Thu, 10 Oct 2013 12:00:00 +0100 https://edoc.ub.uni-muenchen.de/16162/ https://edoc.ub.uni-muenchen.de/16162/1/Avila_Ferrufino_Alejandro.pdf Avila Ferrufino, Alejandro

A scaffolding protein enables clustering of IgG B cell receptors to promote antibody secretion by memory B cells.

Background: Elevated total serum immunoglobulin E (IgE) levels are a prominent feature of allergic and parasitic diseases. An epidemiologic study was conducted in East German children to describe trends in the development of total serum IgE levels and analyze the impact of potential determinants. Methods: The study consisted of three cross-sectional surveys in 1992-1993, 1995-1996 and 1998-1999 and was conducted in three areas of the former German Democratic Republic. In total, 8,051 questionnaires were completed by the parents of children aged 5-14 years, supplying information on allergic symptoms and potential risk factors. A total of 5,918 measurements of total serum IgE and specific IgE to 5 common aeroallergens were available from 4,353 schoolchildren. Generalized estimating equations were applied to data from all children and stratified for atopic and nonatopic children to identify trends and estimate the effect of potential determinants on total IgE. Results: Total serum IgE levels decreased significantly with a linear trend in East German schoolchildren between 1992 and 1999, the effect being stronger in nonatopic children. The following factors were associated with lower total serum IgE levels: female gender, living in a household with fewer than 4 people, no history of helminth infestation, younger age group (5-7 years), no parental allergy and high socioeconomic status. No association was seen for `smoking at home' and close contact to pets. Conclusion: Total serum IgE declined parallel to helminth infestation; however, the latter explained the decrease only in part. Furthermore, total IgE developed in an opposite direction to specific IgE, indicating that it has determinants other than allergic sensitization. Copyright (C) 2011 S. Karger AG, Basel

The electrical activity of the brain is the result of a complex interaction between excitation and inhibition mediated by several types of neurotransmitters. As the majority of neurons in the brain utilize either the inhibitory neurotransmitter γ-aminobutyric acid (GABA) or the excitatory neurotransmitter glutamate, the interplay of these two neurotransmitters principally controls brain excitability and, hence, imbalance between these two neurotransmitters may cause severe pathological conditions. Inhibition of glutamic acid decarboxylase (GAD), the rate-limiting enzyme of GABA synthesis, is believed to change neuronal network activity caused by impaired GABAergic inhibition. Recordings of intrinsic optical signals (IOSs) and whole-cell patch-clamp measurements of GABAA receptor-mediated miniature postsynaptic currents (GABAA Minis) and spontaneous excitatory postsynaptic currents (sEPSCs) were performed in the motor cortex in acute brain slices to unveil the effects of GAD inhibitors at the network level. The first project of this PhD thesis was to prove the IOS technique for its capability of monitoring neuronal network activity over several hours. Concurrently, new software for the analysis of IOS data was developed, which facilitates and significantly accelerates data analysis. Afterwards, changes in neuronal network activity after impairing GAD activity with the well-known GAD inhibitor semicarbazide (SMC) were observed with the IOS technique. If compared to the values of sham-drug application, a stable and reversible increase in both signal intensity and signal area was observed after 2 h of 2 mM SMC application. Consistent with these findings of IOS recordings, patch-clamp measurements of GABAA Minis revealed an SMC-induced reduction in the strength of GABAergic inhibition. The results are in line with the assumption that SMC impairs GABA synthesis by blocking GAD activity. SMC application, however, did not alter spontaneous excitatory neuronal network activity. The final aim of this study was to investigate potential effects of Anti-GAD autoantibodies-containing immunoglobulin G (IgG) derived from patients with stiff-person syndrome (SPS-IgG) on motor cortical neuronal network activity. IOS recordings do not reveal differences in neuronal network activity during SPS-IgG application and control IgG application. However, run-down of IOSs was significantly decelerated during IgG application, which possibly indicates a diminished neuronal cell death caused by an unspecific IgG effect. Compared to brain slices preincubated with IgG-free artificial cerebrospinal fluid, control IgG did not affect GABAA Mini amplitude and frequency as well as sEPSC amplitude. The sEPSC frequency, however, was significantly reduced under these conditions. This decreased excitatory transmitter release might explain the beneficial effect of immunoglobulin treatment in some forms of epilepsy. Similar to SMC, patch-clamp measurements of GABAA Minis revealed a reduction in the strength of GABAergic inhibition after preincubation with SPS-IgG. Consistent with this finding, application of SPS-IgG enhanced sEPSC frequency. This shows that IgG of SPS patients is indeed capable of altering GABAergic synaptic transmission, thus further supporting the hypothesis of an autoimmune origin of the stiff-person syndrome.

A study in the October 2009 CGH looks at presentation and management of post-treatment relapse in autoimmune pancreatitis (AIP) and immunoglobulin G4–associated cholangitis.

Host: Todd Mahr, MD Guest: Stanley Fineman, MD, MBA Immunoglobulin replacement therapy has been used for treating various infections over the past 50 years. It has been traditionally administered intravenously, but now subcutaneous delivery of immunoglobulin is proving to be an attractive alternative. What disorders are immunoglobulin therapy used to treat, and what are the advantages to each type of preparation? Dr. Stanley Fineman, clinical associate professor in the department of pediatrics at Emory University School of Medicine, discusses novel approaches to immunoglobulin therapy with host Dr. Todd Mahr. Which patients are the best candidates for immunoglobulin therapy?

Host: Todd Mahr, MD Guest: Stanley Fineman, MD, MBA Immunoglobulin replacement therapy has been used for treating various infections over the past 50 years. It has been traditionally administered intravenously, but now subcutaneous delivery of immunoglobulin is proving to be an attractive alternative. What disorders are immunoglobulin therapy used to treat, and what are the advantages to each type of preparation? Dr. Stanley Fineman, clinical associate professor in the department of pediatrics at Emory University School of Medicine, discusses novel approaches to immunoglobulin therapy with host Dr. Todd Mahr. Which patients are the best candidates for immunoglobulin therapy?

Tue, 11 Dec 2007 12:00:00 +0100 https://edoc.ub.uni-muenchen.de/7888/ https://edoc.ub.uni-muenchen.de/7888/1/Lech_Maciej.pdf Lech, Maciej ddc:600

Tue, 17 Dec 2002 12:00:00 +0100 https://edoc.ub.uni-muenchen.de/676/ https://edoc.ub.uni-muenchen.de/676/1/Ostermann_Georg.pdf Ostermann, Georg

Sat, 1 Jan 1994 12:00:00 +0100 http://epub.ub.uni-muenchen.de/4067/ http://epub.ub.uni-muenchen.de/4067/1/4067.pdf Cook, Deborah R.; Maxwell, I. H.; Glode, L. M.; Maxwell, F.; Stevens, J. O.; Purner, M. B.; Wagner, Ernst; Curiel, David T.; Curiel, Tyler J. Cook, Deborah R.; Maxwell, I. H.; Glode, L. M.; Maxwell, F.; Stevens, J. O.; Purner, M. B.; Wagner, Ernst; Curiel, David T. und Curiel, Tyler J. (1994): Gene therapy for B-cell lymphoma in a SCID mouse model using an immunoglobulin-regulated diphtheria toxin gene delivered by a novel adenovirus-polylysine conjugate. In: Cancer B

Fri, 1 Jan 1993 12:00:00 +0100 https://epub.ub.uni-muenchen.de/8183/1/improvement_of_the_predictive_value_of_cd4plus_lymphocyte_count_8183.pdf Bogner, Johannes R.; Koch, Meinrad A.; Burkowitz, Jörg; Koch, Judith; Skarabis, Horst; Bek, Barbara; Schwartländer, Bernhard

The localization of Vκ gene regions to chromosome 2, on which the κ locus is located, and to other chromosomes is described. The Vκ genes that have been transposed to other chromosomes are called orphons. The finding of two new Vκ genes on chromosome 22 is reported. A Vκ II gene of this region and two Vκ I genes of the Chr 1 and the cos 118 regions were sequenced. The two Vκ I orphon sequences and two others that had been determined previously were 97.5% identical, indicating that they may have evolved from a common ancestor by amplification. A model of the evolution of the human Vκ orphons is discussed. Author Keywords: Human-rodent cell hybrids; cosmids; restriction maps; ligation artifacts; orphon; recombinant DNA Abbreviations: aa, amino acid(s); bp, base pair(s); Chr1, Vκ gene-containing regions of chromosomes 1; Chr22, Vκ gene-containing regions of chromosomes 22; FR, framework regions; CDR, complementary determining regions; kb, kilo-base(s) or 1000 bp; L, L′, parts of a leader gene segment; m219-1, the first subclone of the cosmid clone cos 219; orphon, Vκ gene outside the κ locus on chromosome 2pl2; SSC, 0.15 M NaCl, 0.015 M Na3-citrate, pH 7.6; V, variable gene segments; J, joining gene segments; C, constant gene segments; Vκ I to Vκ IV, variable gene segments of immunoglobulin light chains of the κ type belonging to subgroups I to IV; for reasons of simplicity Vκ gene segments are generally called Vκ genes

Thu, 1 Jan 1987 12:00:00 +0100 https://epub.ub.uni-muenchen.de/8563/1/8563.pdf Scriba, Peter Christian; Bay, V.; Engel, U.; Mansky, T.; Dwenger, M.; Wenzel, B. E.

We have determined the localization of c-myc and the immunoglobulin kappa light chain genes on the 8q+/2p- chromosomes of the three Burkitt lymphoma lines BL21, LY66 and LY91 with t(2;8) translocation by in situ hybridization. BL21 is characterized by a complex translocation in which a piece of chromosome 9 appears to be located between the fragments of chromosome 8 and 2 on the 8q+ chromosome. Our data indicate that in all three cell lines the c-myc gene is located on the 8q+ chromosome proximal to the breakpoint in band 8q24. In all cell lines examined the cluster of kappa variable genes has remained on the 2p- chromosome. In LY91 cells the major part of the joining region remained on 2p-, while the joining region has moved to 8q+ in the cell lines BL21 and LY66. In all three cell lines the constant kappa light chain gene was found on the 8q+ chromosome. The fact that an essentially identical pattern was found in the cell line BL21, with the complex translocation, suggests that the insertion of the piece of chromosome 9 into the 8q+ chromosome might be a secondary event. Our present data fit into the concept that in all Burkitt lymphoma lines investigated so far, including cases with t(8;14) and the variant translocations t(2;8) and t(8;22), the c-myc gene becomes situated at the 5' side of an immunoglobulin constant gene. This may have implications for the generation of somatic mutations in the coding and non-coding part of the c-myc gene.