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A version of this essay has been published by Open Magazine at https://openthemagazine.com/world/india-will-collapse-without-digital-sovereignty-and-pax-indica-lessons-from-hormuzBy now it is clear that the Iran War (or West Asia War) has been a disaster to all concerned, including the principals as well as assorted passersby. The massive amounts spent by the US (at last count $25 billion) are at least articulated; the bill for the enormous infrastructural and human suffering inflicted on Gulf states, in the theater of war, must be greater, by definition.The collateral damages suffered by the rest of the world from the cessation of trade through the Straits of Hormuz will presumably run into the trillions of dollars. As one of the worst affected, India, which imports 90% of its hydrocarbons from the Gulf, not to mention other essential items such as urea (for fertilizer), sulfuric acid, helium, etc., is on track to take a massive hit. As an article in The Economic Times said, “India must brace for broad-based economic shock”.Indian exports of up to $50 billion are also affected, especially agricultural products including perishable foodstuffs, but also gems and jewellery, electronics, textiles and garments. Some of this can be diverted via Oman and the UAE's Fujairah port, but much of it passes through the Straits of Hormuz and is potentially blocked and/or stranded at sea.The Hormuz closure is a body blow to India's economy. What can and will India do about it? The Indian State has a habit of rising to the challenge only when there is a crisis, while vegetating otherwise. The 1991 economic crisis is a case in point; the sanctions following “The Buddha is smiling”, and the denial of cryogenic rocket engines and supercomputers are other examples where the nation rallied. So were covid vaccines. Necessity, they say, is the mother of invention.Turning a threat into an opportunityIf I were to be an optimist, I could say that the current crisis is actually an opportunity. In fact, a major opportunity. My reading of the Iran War is that it is President Trump's strategic tit-for-tat against China for denying him rare earths and cutting off soybean purchases. In return Trump decided to deny China access to oil by closing access to Venezuela and Iran. Whether this will work, or whether the G2 condominium (read ‘surrender') will prevail, is unclear.But that is, in a sense, background noise that needs to be managed. India needs to focus on its own issues, of which I see several as critical, and the solution in general is to become Atmanirbhar, self-reliant, and from that, to create an Anti-Fragile nation:* National security/defense* Food security* Energy security* Digital security/narrative control* Trade securityThe first three do not need an explanation: they are obvious. Internal and external security are pre-requisites for any successful society. If India's hard-won food security can be threatened by external threats, then there needs to be some deep introspection. Energy security means diversification, both of hydrocarbon sources, and of types of energy, including renewables, nuclear, biomass, coal-based, and so on.Malign narratives and digital sovereigntyNarrative control is something that the Indian State has failed at so far; it is laughably easy to create hate speech against Indians and India (as has been demonstrated freely by any number of players, starting from the MAGA crowd, to Audrey Truschke to a”Cockroach Janata Party” and some nitwit Norwegian journalist in just the last fortnight) and there are no consequences to the culprits. It's enough to make me pine for Lee Kuan Yew's aggressive legal battles against the media.It's one thing if it were only a problem with foreigners, but with the massive spread of social media, and in particular generativeAI, it is becoming a serious domestic issue. Since India is an avid consumer of social media, and because generativeAI is trained on things like Wikipedia, X, Whatsapp and Google content, biased and motivated material becomes ensconced as The Truth. I have written about narrative warfare and manufacturing consent.This used to be a one-way tsunami of (mis)-information by legacy media, but now there is also the opposite: the wholesale and free vacuuming-up of Indian data (whatever happened to “data is the new oil”?). The “Great Firewall of China” both kept out foreign BIg Tech applications and prevented their plundering Chinese data: is that the way to go?Manufactured narratives are intended for regime change: all the color revolutions today are hatched with massive bot-farms funded by some combination of Deep State, CCP, ISI, Qatar etc. (for example the alleged Gen-Z uprisings that rocked Nepal, drove Sheikh Hasina out of Bangladesh). Thus muzzling malign narratives, and ensuring data security, are imperative.Even Singapore is not immune: it had to block anti-India narratives that likely originated from Chinese sources.A particularly striking example of narrative warfare is the virtual hate speech inducted into Wikipedia by deeply prejudiced anonymous editors. Ashley Rindsberg, who exposed the mighty New York Times' biases in his book The Gray Lady Winked, provides many examples of this.Of note to Indians and Hindus is his recent substack titled “Wikipedia's India War” where he identifies just four editors as having created most of the content condemning the Hindu American Foundation (HAF) in ‘Wikivoice', i.e. the allegedly neutral perspective of Wikipedia. They are, on the contrary, shown to be highly one-sided.As Rindsberg mentions, Wikipedia being central to generativeAI, the damage is baked into the world-view of all AI applications. Truly Orwellian. Says Rindsberg: “four… anonymous accounts can have an enormous impact on what millions of people believe to be the truth.” “Over four years (2021-2025), editors systematically erased HAF's identity as an American civil rights group, transforming its Wikipedia page into a heavily curated dossier of accusations.”Trade, and how the Spice Route was far superior to the Silk RoadFinally, something that is becoming increasingly important: ensuring freedom of trade. This is more than just freedom of navigation, although I find it instructive that Emperor Rajendra Chola sent a huge fleet 1,001 years ago simply to open up the Straits of Malacca. India can make an active attempt to regain primacy in Indian Ocean trade, the whole Pax indica idea.Here is another example of the power of narrative: we have been led to believe that the Silk Road to China was some major highway of commerce between ancient Rome and ancient China, but it was a term coined only in 1877 by the German Ferdinand von Richthofen. There was no highway. A large caravan might take six months, and with 500 camels traversing treacherous deserts and braving bandits, it might carry a maximum of 100 tons. That is puny.In comparison, on the Spice Route, a single stitched ship from Muziris could carry 400 tons of ivory, pepper, silk, tigers and elephants; and the historian Strabo around 1 CE talks about fleets of 250 ships going from Alexandria to India on a six-week monsoon-powered journey. That is 100,000 tons of merchandise. No wonder Pliny the Elder complained that Rome's treasuries were being emptied of gold by India.Simple question: where are hoards of ancient Roman coins found in Asia? Answer: not along the Silk Road. The hoards are in Kerala, Tamil Nadu and Sri Lanka.Today, it is possible for India to aspire to port-led development of trade, especially with the major ports at Trivandrum (Vizhinjam), Maharashtra (Vadhavan), and Great Nicobar (Galathea Bay). The underlying ‘software' of India's millennia-old trade competency was a ‘multi-protocol switch' as I pointed out, and today's India Stack can replicate that. Then there is the need for a blue-water navy: muscle to provide security on the Hormuz to Malacca sea-lanes.So there is a vision. How can India get there? This is where policy matters, as I discussed with policy expert Anuj Gupta. Policy, especially industrial policy, has had a bad reputation in certain circles because it was deemed to violate the virginal purity of classical capitalism. However, in a recent U-turn, even the World Bank admitted that industrial policy may not be all that bad, after all: the success of Japan, the Asian Tigers, and China can't be ignored.That leads to the question of why policy in India has produced mediocre outcomes, what is different now, and where the best use of policy might be.Industrial Policy: What went wrong in the past?There are many problems here. To begin with, the Soviet model, which Nehruvians swore by, was, in hindsight, a dead end. Second, there is the problem of governance: post-Independence bureaucrats have awkwardly borne the legacy of imperial hauteur and the needs of a developing society. Third, until recently, the bare necessities (food, electricity, road access) were not available to many citizens, and GDP growth was not their priority.There is also the culture of jugaad: of clever ways in which you overcome constraints through frugal improvisation and seat-of-the-pants making-do. This is fine for one-off things (e.g. converting a tractor trailer into a makeshift transport vehicle because your truck broke down), but it does not make for efficient and replicable industrial products. As The Economic Times said recently, it is time to junk jugaad. Quality has to become ingrained in people's minds.The issue of governance is significant: the bureaucracy and the judiciary have both under-performed, politicians, as everywhere, have been venal. It is said that China's growth can be attributed to the fact that its babus are engineers, and therefore with engineering ruthlessness move in straight lines. The US' babus are lawyers, and India's are humanities graduates. Well, engineers are not very good at second-order effects (eg. China's lurch from one-child policy to demographic collapse), but a little bit of ruthlessness is probably good.What is going reasonably well?There are a few modest success stories: for example, in electronics manufacturing or assembly. The PLIs (and DLIs) have produced the desired effort, with clusters of excellence where global suppliers have also set up shop (as they did earlier for the automobile industry in, say, Sriperumpudur). The fact that a lot of iPhones in the US are now imported from India is laudable, even though it may be derided as “screwdriver jobs”. That's where one starts the move up the value chain.The current semiconductor policy is a big hope, especially after the landmark agreement by the Dutch firm ASML with Tata Electronics in Dholera, Gujarat. Given that ASML has a near-monopoly position in Deep Ultraviolet Lithography (DUV) this is a major boost to India's chip ambitions. My recent conversation with AMD CTO Suraj Rengarajan went into India's chances to realize its ambitions.A recent announcement from Trivandrum-based fabless startup NetraSemi (a recipient of DLI) of the commercial availability of its edge AI chips is a landmark.Next is the newly announced plan for energy security revolving around both coal gasification and intensive offshore exploration. These fall squarely into the Atmanirbhar category: India simply cannot afford to have its energy held hostage by distant nations. It also needs distinctly Indian innovation.The Samudra Manthan initiative is also showing some promise. At least one out of three deep-water wells in the Andaman Sea (SriVijaya Puram-3) are reported to be showing the availability of natural gas, although it will take 5-10 years for this to be commercially available.What should the future look like for India's Industrial Policies?This of course is the hard question. Here is my personal perspective, and I accept that reasonable people may disagree. I think three areas need to be focused on, and will pay large dividends.* Drones and swarming software* Social media and AI stack* Maritime Trade and Blue-Water NavyI admit that these are not the only worthwhile industrial policies. Another is for copper, which would reverse the catastrophic effects of the closure of the Sterlite plant in Thoothukkudi, as the metal is an increasingly important component in electronics, data centers, etc., and far from being self-sufficient earlier, India now imports 50% of its needs. Another area of interest in quantum computing.There are also failures from which the right lessons need to be learned. The policy for EV batteries has apparently failed: according to Swarajya magazine, India has not been able to escape from near-total dependence on imported Chinese batteries.Drone swarmsI wrote recently that drones may well herald a step-change in warfare. For the moment, though, they are searching for their niche in offensive/defensive warfare. Drone hardware is already a well-trodden path with Chinese and other nations dominating it, although with IdeaForge, Paras, Garuda, IoTechworld Avigation etc., India is also making progress there. And India is indeed buying the hardware, $2 billion-worth, according to the Economic Times.But I believe the real game is in drone swarms. AI-based control software (similar to HiveMind) that would allow an entire swarm to act autonomously, just like a murmuration of starlings, would be the gold standard to aim for. Such a self-managing swarm would be virtually impossible to defend against, and I think India should put in place a PLI to support it, leveraging software capability in the country.Of course, drones are not just for military purposes, but also for commercial uses including things like logistics and agricultural use, such as precision delivery of fertilizer and pesticide to crops (as Garuda demonstrates). An Indian initiative that supports both drone hardware, and especially drone software, would be a potential winner.Digital Sovereignty: Social media and AI stackThere is a raging battle over which part of the AI stack India needs to invest in. As an old Unix hand, I believe the foundational model is not where the differentiation is. In analogy with Linux (the open-source Unix variant that was popularized by Linus Torvalds and an army of volunteers), there is little value in re-writing the operating system, but one can differentiate by building on top of it, or by judiciously choosing certain modules of it.Besides, the cost of building an entirely new foundational model would be astronomical and would consume the entire budget of IndiaAI Mission.Thus, my personal opinion is that the foundational model (especially when, it is believed, there are more or less open-source models available for free, e.g. Llama, DeepSeek) is not where India should expend its precious R&D resources, but on the layers of the stack above it. It is the data that matters, as Larry Ellison apparently suggests too.But there is the interesting counter-example of Sarvam AI which is producing its own sovereign model: multi-lingual and presumably otherwise tuned to Indian needs. The question is whether this can survive when hundreds of billions worth of capital investment are going to the US Big Tech companies and their Chinese rivals. The sad history of Koo, a Twitter rival, comes to mind. So does Arattai, a Whatsapp rival, whose popularity has waned. .A well-thought-through industrial policy on generativeAI is therefore essential. The status quo ante is unsustainable; given the fact that Sarvam has also found it difficult to raise funds in the US, it is worth pondering whether a China-style massive subsidy is the answer. And where should it go, into foundational models or into the layers of the stack above it? The answer is “both”, but with priority to the latter.Here is where I would prioritize investments, in order:* Vertical applications in specific domains: e.g. defense, healthcare, agriculture, governance (particularly in the judiciary and in ease of doing business in the bureaucracy)* Fine-tuning and customization: for the needs of the Indian context, e.g. multi-linguality under Bhashini* Compute infrastructure: GPUs, sovereign and protected indian datasets* Sovereign Small-Language Models such as Sarvam AIAs mentioned above, at the moment India's data is being sucked up for free by US Big Tech. In addition, there is the real danger that Indic Knowledge Systems will be mined and digested, as has happened to yoga, pranayama, etc., which have been given Western analogs and nomenclature, as in Pilates, ‘coherent breathing' etc.These two problems are connected, and both need to be tackled in parallel. Social media is being weaponized against India, and this is magnified by the legacy media in a positive feedback loop. Three examples: one was the rage against Adani based on the dubious research of Hindenburg, which then went under; the second is Bloomberg's reckless accusation about gold reserves being sold by the RBI, which they were forced to retract, but social media and Wikipedia will remember it; the third is the meteoric (media) rise of the Cockroach Janata Party.Trade using major ports, Digital Public Infrastructure and a blue water navyUsing trade for competitive advantage is an age-old tactic. The trade tiffs between the US and China are examples of this: we are witnessing war by other means. Many nations are getting into this act, and India does have some advantages, partly based on geography. Maritime trade is likely to continue to be the key, which makes naval chokepoints the big story, but not the only story to watch out for.The major aspects of maritime trade include infrastructure, the digital “multi-protocol switch”, and security. On the one hand, India is developing not only major container ports, and the road/rail links to get to them, and the industrial goods to ship out through them, but also a serious shipbuilding industry, which was one of India's historical strengths. Then it used to be stitched wooden ships (teak beams lashed together with coconut rope). Now it's modern steel ships.There are the big, efficient new ports, which can now turn ships around with Singapore-like efficiency; the proposed third aircraft carrier group which will make it possible to patrol the Arabian Sea and the Bay of Bengal at the time; the Air-Independent Propulsion diesel submarines and nuclear submarines that can monitor (and if necessary, deny) narrow straits; the sale of supersonic Brahmos cruise missiles to the Philippines, Vietnam and Indonesia (and Cyprus) that create ship-denial zones: all this is muscle.And the final piece, the ‘software' for trade, the “multi-protocol switch”. This last is complicated. Its value is underestimated by many. But this is what enables friction-less transactions between various unrelated parties. The India Stack and the Digital Public Infrastructure can be utilized to provide such a facility. But it is complex enough to need significant study as to what is possible, and how to roll it out.Second-order effectsIn closing, it is worth considering some of what the (unintended) consequences of these proposals may be. Let us note that the G2 has no interest in allowing India to grow and make it a G3. They will do everything in their power to kneecap India, by all means possible.There is also a certain derision for India in some circles. Here is a generic western opinion on why China got rich, and India didn't. Well, the author doesn't consider the second-order effects of the wholesale destruction of Chinese civilization: that is a tradeoff Indians may not prefer for themselves. We all know how China's well-intentioned One Child Policy turned into demographic collapse within a few years. Besides, as The Economist asks, “China is innovative. Its economy is a mess. Which will win out?”This is why I think planning for these second-order effects is important. We tend to ignore them because they seem counterintuitive or unlikely, but Nassim Taleb has sensitized us to how low-probability Black Swan events can have grave consequences.As an example, attempting digital sovereignty may have unwelcome side-effects: Big Tech have the first-mover advantage and network effects and there are increasing returns to scale. They will surely make it hard for a new player to break in. Besides, the large investments in data centers and GCCs that they are making in India would make it very difficult for them to be ejected with a “Great Indian Firewall”.Even taxing their capture of Indian data will be complicated; not to mention that they have demonstrated that they can happily violate copyright laws with no consequence; therefore they will find ways to chew up and spit out Indian Knowledge Systems, and essentially re-colonize India. Digital colonialism is not a threat, it is a reality today, and it is a consequence of the relatively open Indian system.In addition, there is a malign group, the “barbarians within” as Arnold Toynbee once put it, who are ready to sacrifice Indian sovereignty for a pittance.Given all this, it will be very difficult to put in place serious measures to gain digital independence; and the narrative-peddling is likely to gain further momentum: just consider the caste allegations that have haunted BAPS in the US (despite the cases being dismissed by the US DoJ), the Cisco Systems case where, again, the case was dismissed, but the narrative continues, and the persistent efforts in various US states to turn caste into a weapon to bludgeon Indians.Another sensitive issue is that of the multi-protocol switch for trade. While from an Indian point of view, it eases trade and harks back to a Golden Age of Indic maritime commerce, but that will be viewed elsewhere very differently, for instance by the US as an attempt to de-dollarize. The US has jealousy guarded – with very good reasons that we will not go into here – the dollar's reserve currency status.We have also seen what happened to those who attempt to hurt the dollar's primacy: in 1985, the Plaza Accord devalued the dollar, and that was a body blow to Japan's economy, which has not recovered its mojo to this day. Later, Iraq's Saddam Hussein and Libya's Muammar Gaddafi both had ideas about replacing the petro-dollar with, respectively, the Euro and a new pan-African gold-backed currency. We know what happened to them.If the India Stack multi-protocol switch is perceived as an alternative to the US dollar, there may be grave consequences. Therefore, it should be conceived and deployed only as an adjunct to it and to the almighty SWIFT settlement system.ConclusionIndia is at a crossroads now. Even though the Hormuz closure is a serious problem, if it plays its cards right, adversity can be turned into opportunity across a variety of perspectives. The key is Atmanirbhar, self-reliance. If India can now implement a crash program of industrial policy, and at the same time overcome an ingrained Third-World tendency to cut corners, it can finally break free of the years of underperformance, what I called the Nehruvian Penalty in 2004.It is possible, but there are caveats: unforeseen consequences. Hic sunt dracones. Here be dragons. Be afraid. Be very afraid.3700 words, 7 June 2026This is episode 192 of the Shadow Warrior podcast. Here is a companion AI-generated slideshow. (Note that the borders of India are not necessarily depicted correctly here, because it is generated by an AI, notebookLM.google.com) This is a public episode. If you'd like to discuss this with other subscribers or get access to bonus episodes, visit rajeevsrinivasan.substack.com/subscribe

56 For my flesh is meat indeed: and my blood is drink indeed.Caro enim mea vere est cibus : et sanguis meus, vere est potus; 57 He that eateth my flesh, and drinketh my blood, abideth in me, and I in him.qui manducat meam carnem et bibit meum sanguinem, in me manet, et ego in illo. 58 As the living Father hath sent me, and I live by the Father; so he that eateth me, the same also shall live by me.Sicut misit me vivens Pater, et ego vivo propter Patrem : et qui manducat me, et ipse vivet propter me. 59 This is the bread that came down from heaven. Not as your fathers did eat manna, and are dead. He that eateth this bread, shall live for ever.Hic est panis qui de caelo descendit. Non sicut manducaverunt patres vestri manna, et mortui sunt. Qui manducat hunc panem, vivet in aeternum.The Eucharist is instituted in the form of food, so that we may receive in Holy Communion the Victim of the Cross.

44 No man can come to me, except the Father, who hath sent me, draw him; and I will raise him up in the last day.nemo potest venire ad me, nisi Pater, qui misit me, traxerit eum; et ego resuscitabo eum in novissimo die. 45 It is written in the prophets: And they shall all be taught of God. Every one that hath heard of the Father, and hath learned, cometh to me.Est scriptum in prophetis : Et erunt omnes docibiles Dei. Omnis qui audivit a Patre, et didicit, venit ad me. 46 Not that any man hath seen the Father; but he who is of God, he hath seen the Father.Non quia Patrem vidit quisquam, nisi is, qui est a Deo, hic vidit Patrem. 47 Amen, amen I say unto you: He that believeth in me, hath everlasting life.Amen, amen dico vobis : qui credit in me, habet vitam aeternam. 48 I am the bread of life.Ego sum panis vitae. 49 Your fathers did eat manna in the desert, and are dead.Patres vestri manducaverunt manna in deserto, et mortui sunt. 50 This is the bread which cometh down from heaven; that if any man eat of it, he may not die.Hic est panis de caelo descendens : ut si quis ex ipso manducaverit, non moriatur. 51 I am the living bread which came down from heaven.Ego sum panis vivus, qui de caelo descendi. 52 If any man eat of this bread, he shall live for ever; and the bread that I will give, is my flesh, for the life of the world.Si quis manducaverit ex hoc pane, vivet in aeternum : et panis quem ego dabo, caro mea est pro mundi vita.[44] "Draw him": Not by compulsion, nor by laying the free will under any necessity, but by the strong and sweet motions of his heavenly grace.

 46 He came again therefore into Cana of Galilee, where he made the water wine. And there was a certain ruler, whose son was sick at Capharnaum.Venit ergo iterum in Cana Galilaeae, ubi fecit aquam vinum. Et erat quidam regulus, cujus filius infirmabatur Capharnaum. 47 He having heard that Jesus was come from Judea into Galilee, went to him, and prayed him to come down, and heal his son; for he was at the point of death.Hic cum audisset quia Jesus adveniret a Judaea in Galilaeam, abiit ad eum, et rogabat eum ut descenderet, et sanaret filium ejus : incipiebat enim mori. 48 Jesus therefore said to him: Unless you see signs and wonders, you believe not.Dixit ergo Jesus ad eum : Nisi signa et prodigia videritis, non creditis. 49 The ruler saith to him: Lord, come down before that my son die.Dicit ad eum regulus : Domine, descende priusquam moriatur filius meus. 50 Jesus saith to him: Go thy way; thy son liveth. The man believed the word which Jesus said to him, and went his way.Dicit ei Jesus : Vade, filius tuus vivit. Credidit homo sermoni quem dixit ei Jesus, et ibat. 51 And as he was going down, his servants met him; and they brought word, saying, that his son lived.Jam autem eo descendente, servi occurrerunt ei, et nuntiaverunt dicentes, quia filius ejus viveret. 52 He asked therefore of them the hour wherein he grew better. And they said to him: Yesterday, at the seventh hour, the fever left him.Interrogabat ergo horam ab eis in qua melius habuerit. Et dixerunt ei : Quia heri hora septima reliquit eum febris. 53 The father therefore knew, that it was at the same hour that Jesus said to him, Thy son liveth; and himself believed, and his whole house.Cognovit ergo pater, quia illa hora erat in qua dixit ei Jesus : Filius tuus vivit; et credidit ipse et domus ejus tota.Nereus and Achilleus were servants of Flavia Domitilla, who was martyred with them in A.D. 98. St Pancras was put to death at the age of fourteen. A.D. 304.

Andrew Clugston, PhD, a postdoctoral fellow in the Sweet-Cordero Lab at UCSF joins us on OsteoBites to discuss his OutSmarting Osteosarcoma funded work, in partnership with the RISE Foundation, on defining tumor-specific vulnerabilities by mapping oncogenic structural variation in osteosarcoma.The genomes of osteosarcoma (OS) cancer cells are among the most complex cancer genomes ever observed. Initially formed by hundreds to thousands of incorrectly repaired DNA breaks (structural variants; SVs), OS genomes contain DNA structures that are unique to that patient and tumor cell, combining genes and regulatory features from across the genome in ways that effectively re-wire that cell's gene expression mechanisms. OS cells are also susceptible to further SVs over time and in response to treatment, potentially allowing OS tumors to evolve rapidly. But these complex and tumor-specific genomic structures may also harbor tumor-specific vulnerabilities. By mapping the many unique DNA structures among patient-derived OS tumor cells, Dr. Clugston has attempted to demonstrate that it is possible both to describe the essential structures within these cells and to search them for novel target genes vulnerable to existing drugs and treatment protocols. Using chromatin conformation capture sequencing (HiC) to observe the shape of the genome and optical genome mapping (OGM) to identify tumor-specific DNA structures, Dr. Clugston has produced tumor-specific maps for multiple patient-derived OS tumor cell lines and has begun development of a search process based on long-read mapping techniques that he hopes will inform future patient-specific treatment protocols.Andrew Clugston grew up in the small town of Lake Luzerne, New York. He received a BS in Biochemistry and an MS in Chemistry at the Rochester Institute of Technology, and he received his PhD in Integrative Systems Biology at the University of Pittsburgh. During his PhD he learned to use and develop bioinformatics tools and techniques to study the role of the genome in kidney as well as eye development, and in the process became fascinated with the importance of 3-dimensional organization in regulating cell behavior. Andrew has since joined the Sweet-Cordero laboratory in the Pediatric Oncology Division at the University of California San Francisco as a Postdoctoral Fellow. There, he applies his knowledge and skillset to study how disruptions in these organizational principles allow osteosarcoma cells to develop and proliferate, and how these changes reveal tumor-specific vulnerabilities that can be exploited for fast and effective treatment options that improve the lives of patients.

1 And there was a man of the Pharisees, named Nicodemus, a ruler of the Jews.Erat autem homo ex pharisaeis, Nicodemus nomine, princeps Judaeorum. 2 This man came to Jesus by night, and said to him: Rabbi, we know that thou art come a teacher from God; for no man can do these signs which thou dost, unless God be with him.Hic venit ad Jesum nocte, et dixit ei : Rabbi, scimus quia a Deo venisti magister, nemo enim potest haec signa facere, quae tu facis, nisi fuerit Deus cum eo. 3 Jesus answered, and said to him: Amen, amen I say to thee, unless a man be born again, he cannot see the kingdom of God.Respondit Jesus, et dixit ei : Amen, amen dico tibi, nisi quis renatus fuerit denuo, non potest videre regnum Dei. 4 Nicodemus saith to him: How can a man be born when he is old? can he enter a second time into his mother's womb, and be born again?Dicit ad eum Nicodemus : Quomodo potest homo nasci, cum sit senex? numquid potest in ventrem matris suae iterato introire et renasci? 5 Jesus answered: Amen, amen I say to thee, unless a man be born again of water and the Holy Ghost, he cannot enter into the kingdom of God.Respondit Jesus : Amen, amen dico tibi, nisi quis renatus fuerit ex aqua, et Spiritu Sancto, non potest introire in regnum Dei. 6 That which is born of the flesh, is flesh; and that which is born of the Spirit, is spirit.Quod natum est ex carne, caro est : et quod natum est ex spiritu, spiritus est. 7 Wonder not, that I said to thee, you must be born again.Non mireris quia dixi tibi : oportet vos nasci denuo. 8 The Spirit breatheth where he will; and thou hearest his voice, but thou knowest not whence he cometh, and whither he goeth: so is every one that is born of the Spirit.Spiritus ubi vult spirat, et vocem ejus audis, sed nescis unde veniat, aut quo vadat : sic est omnis qui natus est ex spiritu. 9 Nicodemus answered, and said to him: How can these things be done?Respondit Nicodemus, et dixit ei : Quomodo possunt haec fieri? 10 Jesus answered, and said to him: Art thou a master in Israel, and knowest not these things?Respondit Jesus, et dixit ei : Tu es magister in Israel, et haec ignoras? 11 Amen, amen I say to thee, that we speak what we know, and we testify what we have seen, and you receive not our testimony.amen, amen dico tibi, quia quod scimus loquimur, et quod vidimus testamur, et testimonium nostrum non accipitis. 12 If I have spoken to you earthly things, and you believe not; how will you believe, if I shall speak to you heavenly things?Si terrena dixi vobis, et non creditis : quomodo, si dixero vobis caelestia, credetis? 13 And no man hath ascended into heaven, but he that descended from heaven, the Son of man who is in heaven.Et nemo ascendit in caelum, nisi qui descendit de caelo, Filius hominis, qui est in caelo. 14 And as Moses lifted up the serpent in the desert, so must the Son of man be lifted up:Et sicut Moyses exaltavit serpentem in deserto, ita exaltari oportet Filium hominis : 15 That whosoever believeth in him, may not perish; but may have life everlasting.ut omnis qui credit in ipsum, non pereat, sed habeat vitam aeternam.[5] "Unless a man be born again": By these words our Saviour hath declared the necessity of baptism; and by the word water it is evident that the application of it is necessary with the words. Matt. 28. 19.After the victory gained by Constantine by virtue of the cross which appeared to him in the skies, and whose sign he reproduced in the Labarum, St Helena, his mother, went to Jerusalem to try to find the true Cross. At the beginning of the second century, Hadrian had covered Calvary and the Holy Sepulchre under of terrace of 300 feet in length, on which had been erected a statue of Jupiter and and temple of Venus. The Empress razed them to the ground, and, in digging up the soil, they discovered the nails and the glorious trophy of which we owe "life salvation and resurrection". The miraculous cure of a woman authenticated the sacred tree.

Una tesis de la Universidad de Deusto advierte del riesgo de automatizar la injusticia si no aplicamos un marco feminista y de derechos humanos a la tecnología. Borja Sanz Urquijo, profesor e investigador en la Universidad de Deusto, publica ‘Hic et nunc', una tesis que insta a la acción inmediata, al considerar que estamos en un momento crítico para sentar las reglas de la IA que gobernará nuestro futuro

Enjoyed our podcast? Shoot us a text and let us know—because great conversations never end at the last word!This week on TezTalks Radio, host Brandon Langston sits down with Ryan Tanaka, a longtime builder in the Tezos art ecosystem behind projects like TEIA, teia.cafe, and Tezcon.The conversation starts with a moment many remember, when Hic et Nunc shut down. For some, that was the end. For others, including Ryan, it became the reason to rebuild.

36 And one of the Pharisees desired him to eat with him. And he went into the house of the Pharisee, and sat down to meat.Rogabat autem illum quidam de pharisaeis ut manducaret cum illo. Et ingressus domum pharisaei discubuit. 37 And behold a woman that was in the city, a sinner, when she knew that he sat at meat in the Pharisee's house, brought an alabaster box of ointment;Et ecce mulier, quae erat in civitate peccatrix, ut cognovit quod accubuisset in domo pharisaei, attulit alabastrum unguenti : 38 And standing behind at his feet, she began to wash his feet, with tears, and wiped them with the hairs of her head, and kissed his feet, and anointed them with the ointment.et stans retro secus pedes ejus, lacrimis coepit rigare pedes ejus, et capillis capitis sui tergebat, et osculabatur pedes ejus, et unguento ungebat. 39 And the Pharisee, who had invited him, seeing it, spoke within himself, saying: This man, if he were a prophet, would know surely who and what manner of woman this is that toucheth him, that she is a sinner.Videns autem pharisaeus, qui vocaverat eum, ait intra se dicens : Hic si esset propheta, sciret utique quae et qualis est mulier, quae tangit eum : quia peccatrix est. 40 And Jesus answering, said to him: Simon, I have somewhat to say to thee. But he said: Master, say it.Et respondens Jesus, dixit ad illum : Simon, habeo tibi aliquid dicere. At ille ait : Magister, dic. 41 A certain creditor had two debtors, the one who owed five hundred pence, and the other fifty.Duo debitores erant cuidam foeneratori : unus debebat denarios quingentos, et alius quinquaginta. 42 And whereas they had not wherewith to pay, he forgave them both. Which therefore of the two loveth him most?Non habentibus illis unde redderent, donavit utrisque. Quis ergo eum plus diligit? 43 Simon answering, said: I suppose that he to whom he forgave most. And he said to him: Thou hast judged rightly.Respondens Simon dixit : Aestimo quia is cui plus donavit. At ille dixit : Recte judicasti. 44 And turning to the woman, he said unto Simon: Dost thou see this woman? I entered into thy house, thou gavest me no water for my feet; but she with tears hath washed my feet, and with her hairs hath wiped them.Et conversus ad mulierem, dixit Simoni : Vides hanc mulierem? Intravi in domum tuam, aquam pedibus meis non dedisti : haec autem lacrimis rigavit pedes meos, et capillis suis tersit. 45 Thou gavest me no kiss; but she, since she came in, hath not ceased to kiss my feet.Osculum mihi non dedisti : haec autem ex quo intravit, non cessavit osculari pedes meos. 46 My head with oil thou didst not anoint; but she with ointment hath anointed my feet.Oleo caput meum non unxisti : haec autem unguento unxit pedes meos. 47 Wherefore I say to thee: Many sins are forgiven her, because she hath loved much. But to whom less is forgiven, he loveth less.Propter quod dico tibi : remittuntur ei peccata multa, quoniam dilexit multum. Cui autem minus dimittitur, minus diligit. 48 And he said to her: Thy sins are forgiven thee.Dixit autem ad illam : Remittuntur tibi peccata. 49 And they that sat at meat with him began to say within themselves: Who is this that forgiveth sins also?Et coeperunt qui simul accumbebant, dicere intra se : Quis est hic qui etiam peccata dimittit? 50 And he said to the woman: Thy faith hath made thee safe, go in peace.Dixit autem ad mulierem : Fides tua te salvam fecit : vade in pace.[47] "Many sins are forgiven her, because she hath loved much": In the scripture an effect sometimes seems attributed to one only cause, when there are divers other concurring dispositions; for the sins of this woman, in this verse, are said to be forgiven, because she loved much: but (ver. 50) Christ tells her, Thy faith hath made thee safe. Hence in a true conversion are joined faith, hope, love, sorrow for sin, and other pious dispositions.

HiC Luttmers joined me this month to talk about Eternal Egypt by Richard J. Reidy, a Bay Area local and founder of the Temple of Ra in San Francisco. We don't get around to talking about writing but we do cover quite about Reidy, Kemetic practice and its adoption to the modern world, and Egyptian death rites.Eternal Egypt was self published but is widely available in print and ebook formats. You can learn more about HiC on their website, which includes all their social media links and information about booking a tarot session with them and subscribing to their newsletter.WitchLit listeners receive 15% off their purchases at La Panthére Studio with the code WITCHLIT.Please support Black, Indigenous, queer, trans, and women-owned, local, independent bookstores and occult shops.Transcripts of all episodes are available at witchlitpod.com.Support WitchLit by using our affiliate link to purchase books from Bookshop.org and follow us on BlueSky for episode updates. You can follow me on Mastodon for daily writing prompts and a tarot or oracle card of the day.You can also support WitchLit by purchasing books published by 1000Volt Press. Our latest release is The Keeper & the Mermaid by Cathy Lynn available wherever you buy ebooks.Death in the Dry River, a crime novella set in 1930s colonial Trinidad, by Lisa Allen-Agostini, the award-winning books Changing Paths by Yvonne Aburrow and Conjuring the Commonplace by Laine Fuller & Cory Thomas Hutcheson are all available from 1000Volt Press or to order wherever you buy books.You can pre-order A Witch's Book of Days (September 2026) from Crossed Crow Books and other booksellers now. My book, Verona Green, is available in all the usual places. Autographed copies are also available from 1000Volt Press.

Chi era Kaspar Hauser, adolescente pallidissimo che sembrava non avere mai vissuto in mezzo agli uomini e che diceva di essere figlio di un cavaliere del Sesto Reggimento di stanza a Norimberga?Non sappiamo il suo vero nome, né la sua origine e nemmeno chi lo abbia infine ucciso. "Hic occultus occulto occisus est", recita un monumento eretto a suo nome... Qual è dunque la verità sul suo conto?Inauguriamo oggi la 35esima stagione, Enigmi e segreti, con una delle storie più misteriose del XIX secolo. Buon ascolto e... ci vediamo il 28 marzo al MUG di Bologna!

 33 Hear ye another parable. There was a man an householder, who planted a vineyard, and made a hedge round about it, and dug in it a press, and built a tower, and let it out to husbandmen; and went into a strange country.Aliam parabolam audite : Homo erat paterfamilias, qui plantavit vineam, et sepem circumdedit ei, et fodit in ea torcular, et aedificavit turrim, et locavit eam agricolis, et peregre profectus est. 34 And when the time of the fruits drew nigh, he sent his servants to the husbandmen that they might receive the fruits thereof.Cum autem tempus fructuum appropinquasset, misit servos suos ad agricolas, ut acciperent fructus ejus. 35 And the husbandmen laying hands on his servants, beat one, and killed another, and stoned another.Et agricolae, apprehensis servis ejus, alium ceciderunt, alium occiderunt, alium vero lapidaverunt. 36 Again he sent other servants more than the former; and they did to them in like manner.Iterum misit alios servos plures prioribus, et fecerunt illis similiter. 37 And last of all he sent to them his son, saying: They will reverence my son.Novissime autem misit ad eos filium suum, dicens : Verebuntur filium meum. 38 But the husbandmen seeing the son, said among themselves: This is the heir: come, let us kill him, and we shall have his inheritance.Agricolae autem videntes filium dixerunt intra se : Hic est haeres, venite, occidamus eum, et habebimus haereditatem ejus. 39 And taking him, they cast him forth out of the vineyard, and killed him.Et apprehensum eum ejecerunt extra vineam, et occiderunt. 40 When therefore the lord of the vineyard shall come, what will he do to those husbandmen?Cum ergo venerit dominus vineae, quid faciet agricolis illis? 41 They say to him: He will bring those evil men to an evil end; and will let out his vineyard to other husbandmen, that shall render him the fruit in due season.Aiunt illi : Malos male perdet : et vineam suam locabit aliis agricolis, qui reddant ei fructum temporibus suis. 42 Jesus saith to them: Have you never read in the Scriptures: The stone which the builders rejected, the same is become the head of the corner? By the Lord this has been done; and it is wonderful in our eyes.Dicit illis Jesus : Numquam legistis in Scripturis : Lapidem quem reprobaverunt aedificantes, hic factus est in caput anguli : a Domino factum est istud, et est mirabile in oculis nostris? 43 Therefore I say to you, that the kingdom of God shall be taken from you, and shall be given to a nation yielding the fruits thereof.Ideo dico vobis, quia auferetur a vobis regnum Dei, et dabitur genti facienti fructus ejus. 44 And whosoever shall fall on this stone, shall be broken: but on whomsoever it shall fall, it shall grind him to powder.Et qui ceciderit super lapidem istum, confringetur : super quem vero ceciderit, conteret eum. 45 And when the chief priests and Pharisees had heard his parables, they knew that he spoke of them.Et cum audissent principes sacerdotum et pharisaei parabolas ejus, cognoverunt quod de ipsis diceret. 46 And seeking to lay hands on him, they feared the multitudes: because they held him as a prophet.Et quaerentes eum tenere, timuerunt turbas : quoniam sicut prophetam eum habebant.

1 And after six days Jesus taketh unto him Peter and James, and John his brother, and bringeth them up into a high mountain apart:Et post dies sex assumit Jesus Petrum, et Jacobum, et Joannem fratrem ejus, et ducit illos in montem excelsum seorsum : 2 And he was transfigured before them. And his face did shine as the sun: and his garments became white as snow.et transfiguratus est ante eos. Et resplenduit facies ejus sicut sol : vestimenta autem ejus facta sunt alba sicut nix. 3 And behold there appeared to them Moses and Elias talking with him.Et ecce apparuerunt illis Moyses et Elias cum eo loquentes. 4 And Peter answering, said to Jesus: Lord, it is good for us to be here: if thou wilt, let us make here three tabernacles, one for thee, and one for Moses, and one for Elias.Respondens autem Petrus, dixit ad Jesum : Domine, bonum est nos hic esse : si vis, faciamus tria tabernacula, tibi unum, Moysi unum, et Eliae unum. 5 And as he was yet speaking, behold a bright cloud overshadowed them. And lo, a voice out of the cloud, saying: This is my beloved Son, in whom I am well pleased: hear ye him.Adhuc eo loquente, ecce nubes lucida obumbravit eos. Et ecce vox de nube, dicens : Hic est Filius meus dilectus, in quo mihi bene complacui : ipsum audite. 6 And the disciples hearing, fell upon their face, and were very much afraid.Et audientes discipuli ceciderunt in faciem suam, et timuerunt valde. 7 And Jesus came and touched them: and said to them, Arise, and fear not.Et accessit Jesus, et tetigit eos : dixitque eis : Surgite, et nolite timere. 8 And they lifting up their eyes saw no one but only Jesus.Levantes autem oculos suos, neminem viderunt, nisi solum Jesum. 9 And as they came down from the mountain, Jesus charged them, saying: Tell the vision to no man, till the Son of man be risen from the dead.Et descendentibus illis de monte, praecepit eis Jesus, dicens : Nemini dixeritis visionem, donec Filius hominis a mortuis resurgat.

 1 And after six days Jesus taketh unto him Peter and James, and John his brother, and bringeth them up into a high mountain apart:Et post dies sex assumit Jesus Petrum, et Jacobum, et Joannem fratrem ejus, et ducit illos in montem excelsum seorsum : 2 And he was transfigured before them. And his face did shine as the sun: and his garments became white as snow.et transfiguratus est ante eos. Et resplenduit facies ejus sicut sol : vestimenta autem ejus facta sunt alba sicut nix. 3 And behold there appeared to them Moses and Elias talking with him.Et ecce apparuerunt illis Moyses et Elias cum eo loquentes. 4 And Peter answering, said to Jesus: Lord, it is good for us to be here: if thou wilt, let us make here three tabernacles, one for thee, and one for Moses, and one for Elias.Respondens autem Petrus, dixit ad Jesum : Domine, bonum est nos hic esse : si vis, faciamus tria tabernacula, tibi unum, Moysi unum, et Eliae unum. 5 And as he was yet speaking, behold a bright cloud overshadowed them. And lo, a voice out of the cloud, saying: This is my beloved Son, in whom I am well pleased: hear ye him.Adhuc eo loquente, ecce nubes lucida obumbravit eos. Et ecce vox de nube, dicens : Hic est Filius meus dilectus, in quo mihi bene complacui : ipsum audite. 6 And the disciples hearing, fell upon their face, and were very much afraid.Et audientes discipuli ceciderunt in faciem suam, et timuerunt valde. 7 And Jesus came and touched them: and said to them, Arise, and fear not.Et accessit Jesus, et tetigit eos : dixitque eis : Surgite, et nolite timere. 8 And they lifting up their eyes saw no one but only Jesus.Levantes autem oculos suos, neminem viderunt, nisi solum Jesum. 9 And as they came down from the mountain, Jesus charged them, saying: Tell the vision to no man, till the Son of man be risen from the dead.Et descendentibus illis de monte, praecepit eis Jesus, dicens : Nemini dixeritis visionem, donec Filius hominis a mortuis resurgat.

10 And when he was come into Jerusalem, the whole city was moved, saying: Who is this?Et cum intrasset Jerosolymam, commota est universa civitas, dicens : Quis est hic? 11 And the people said: This is Jesus the prophet, from Nazareth of Galilee.Populi autem dicebant : Hic est Jesus propheta a Nazareth Galilaeae. 12 And Jesus went into the temple of God, and cast out all them that sold and bought in the temple, and overthrew the tables of the money changers, and the chairs of them that sold doves:Et intravit Jesus in templum Dei, et ejiciebat omnes vendentes et ementes in templo, et mensas numulariorum, et cathedras vendentium columbas evertit : 13 And he saith to them: It is written, My house shall be called the house of prayer; but you have made it a den of thieves.et dicit eis : Scriptum est : Domus mea domus orationis vocabitur : vos autem fecistis illam speluncam latronum. 14 And there came to him the blind and the lame in the temple; and he healed them.Et accesserunt ad eum caeci, et claudi in templo : et sanavit eos. 15 And the chief priests and scribes, seeing the wonderful things that he did, and the children crying in the temple, and saying: Hosanna to the son of David; were moved with indignation.Videntes autem principes sacerdotum et scribae mirabilia quae fecit, et pueros clamantes in templo, et dicentes : Hosanna filio David : indignati sunt, 16 And said to him: Hearest thou what these say? And Jesus said to them: Yea, have you never read: Out of the mouth of infants and of sucklings thou hast perfected praise?et dixerunt ei : Audis quid isti dicunt? Jesus autem dixit eis : Utique. Numquam legistis : Quia ex ore infantium et lactentium perfecisti laudem? 17 And leaving them, he went out of the city into Bethania, and remained there.Et relictis illis, abiit foras extra civitatem in Bethaniam : ibique mansit.

In this episode of the Epigenetics Podcast, we talked with Srinjan Basu from Imperial College London to talk about his work on how chromatin architecture and epigenetic mechanisms orchestrate developmental gene expression programs. We begin by exploring Dr. Basu's early work at Harvard which involved pioneering Raman-based label-free imaging, allowing the study of chromatin dynamics in live tissue. Here, he tackles technical challenges faced in visualizing DNA interactions, emphasizing the shift from 2D to 3D analysis and the importance of real-time observation of chromatin behavior under various conditions. This segues into his groundbreaking research on single transcription factors interacting with chromatin, revealing subtle but significant changes in the dynamics of gene regulation. We transition into the complexities of chromatin architecture as Dr. Basu recounts his efforts in mapping the entire mouse genome in single pluripotent cells, unearthing unexpected heterogeneity among cells. This heterogeneity raises intriguing questions about its impact on cellular function, prompting ongoing investigations into chromatin dynamics and the role of remodeling complexes like NuRD in cell fate transitions. Dr. Basu elucidates how recent studies have begun to bridge the gaps in understanding how transcription factors and chromatin dynamics interact during cellular decisions, particularly emphasizing the influence of mechanical signals and the intrinsic properties of cells. His research underscores the idea that stem cells undergo a preparatory phase for differentiation, highlighting the critical balance of intrinsic and extrinsic factors that govern genetic expression and cellular outcomes. We also talk about Dr. Basu's current research trajectory, focusing on enhancing imaging techniques to study gene dynamics in tissue contexts relevant to developmental biology and disease states. He illustrates a vision for future projects that integrate advanced imaging tools to investigate transcription factor dynamics and chromatin interactions in live cells and embryos, furthering the understanding of decision-making processes in cellular contexts. References Stevens TJ, Lando D, Basu S, et al. 3D structures of individual mammalian genomes studied by single-cell Hi-C. Nature. 2017 Apr;544(7648):59-64. DOI: 10.1038/nature21429. PMID: 28289288; PMCID: PMC5385134. Basu S, Needham LM, Lando D, et al. FRET-enhanced photostability allows improved single-molecule tracking of proteins and protein complexes in live mammalian cells. Nature Communications. 2018 Jun;9(1):2520. DOI: 10.1038/s41467-018-04486-0. PMID: 29955052; PMCID: PMC6023872. Related Episodes Advanced Optical Imaging in 3D Nuclear Organisation (Lothar Schermelleh) Analysis of 3D Chromatin Structure Using Super-Resolution Imaging (Alistair Boettiger) Single-Molecule Imaging of the Epigenome (Efrat Shema) Contact Epigenetics Podcast on Mastodon Epigenetics Podcast on Bluesky Dr. Stefan Dillinger on LinkedIn Active Motif on LinkedIn Active Motif on Bluesky Email: podcast@activemotif.com

 1 And it came to pass, that on one of the days, as he was teaching the people in the temple, and preaching the gospel, the chief priests and the scribes, with the ancients, met together,Et factum est in una dierum, docente illo populum in templo, et evangelizante, convenerunt principes sacerdotum, et scribae cum senioribus, 2 And spoke to him, saying: Tell us, by what authority dost thou these things? or, Who is he that hath given thee this authority?et aiunt dicentes ad illum : Dic nobis in qua potestate haec facis? aut quis est qui dedit tibi hanc potestatem? 3 And Jesus answering, said to them: I will also ask you one thing. Answer me:Respondens autem Jesus, dixit ad illos : Interrogabo vos et ego unum verbum. Respondete mihi : 4 The baptism of John, was it from heaven, or of men?baptismus Joannis de caelo erat, an ex hominibus? 5 But they thought within themselves, saying: If we shall say, From heaven: he will say: Why then did you not believe him?At illi cogitabant intra se, dicentes : Quia si dixerimus : De caelo, dicet : Quare ergo non credidistis illi? 6 But if we say, Of men, the whole people will stone us: for they are persuaded that John was a prophet.Si autem dixerimus : Ex hominibus, plebs universa lapidabit nos : certi sunt enim Joannem prophetam esse. 7 And they answered, that they knew not whence it was.Et responderunt se nescire unde esset. 8 And Jesus said to them: Neither do I tell thee by what authority I do these things.Et Jesus ait illis : Neque ego dico vobis in qua potestate haec facio. 9 And he began to speak to the people this parable: A certain man planted a vineyard, and let it out to husbandmen: and he was abroad for a long time.Coepit autem dicere ad plebem parabolam hanc : Homo plantavit vineam, et locavit eam colonis : et ipse peregre fuit multis temporibus. 10 And at the season he sent a servant to the husbandmen, that they should give him of the fruit of the vineyard. Who, beating him, sent him away empty.Et in tempore misit ad cultores servum, ut de fructu vineae darent illi. Qui caesum dimiserunt eum inanem. 11 And again he sent another servant. But they beat him also, and treating him reproachfully, sent him away empty.Et addidit alterum servum mittere. Illi autem hunc quoque caedentes, et afficientes contumelia, dimiserunt inanem. 12 And again he sent the third: and they wounded him also, and cast him out.Et addidit tertium mittere : qui et illum vulnerantes ejecerunt. 13 Then the lord of the vineyard said: What shall I do? I will send my beloved son: it may be, when they see him, they will reverence him.Dixit autem dominus vineae : Quid faciam? Mittam filium meum dilectum : forsitan, cum hunc viderint, verebuntur. 14 Whom when the husbandmen saw, they thought within themselves, saying: This is the heir, let us kill him, that the inheritance may be ours.Quem cum vidissent coloni, cogitaverunt intra se, dicentes : Hic est haeres, occidamus illum, ut nostra fiat haereditas. 15 So casting him out of the vineyard, they killed him. What therefore will the lord of the vineyard do to them?Et ejectum illum extra vineam, occiderunt. Quid ergo faciet illis dominus vineae? 16 He will come, and will destroy these husbandmen, and will give the vineyard to others. Which they hearing, said to him: God forbid.veniet, et perdet colonos istos, et dabit vineam aliis. Quo audito, dixerunt illi : Absit.St Blaise, bishop of Sebaste, was beheaded after terrible torments, under Licinius, A.D. 317.

Two years without discussing effective altruism -- did you miss it? Not as much as Vaden, surely. And probably a right bit more than Ben. Well, we're back in the game with a spicy one. Was EA a front for AI safety from the beginning? Did the leaders care not a wit for global poverty? Is Ben going to throw himself out window if Vaden keeps this up? We discuss Feedback on our introspection episode The motives of the EA founders The felicia forum Is this a conspiracy theory? EA's strategic ambiguity Bostromism, transhumanism, and AI safety EA funding The public/core divide and the funnel model Quotes new effective altruists tend to start off concerned about global poverty or animal suffering and then hear, take seriously, and often are convinced by the arguments for existential risk mitigation - Will MacAskill Existential risk isn't the most useful public face for effective altruism – everyone inc[l]uding Eliezer Yudkowsky agrees about that - Scott Alexander, 2015 Utilitymonster: GWWC is explicitly poverty-focused but high impact careers (HIC) is not. In fact, hardcore members of GWWC are heavily interested in x-risk, and I estimate that 10-15% of its general membership is as well. I'd take them seriously as a group for promoting utilitarianism in general. I'm a GWWC leader. [Redacted]: but HIC always seems to talk about things in terms of “lives saved”, ive never heard them mentioning other things to donate to. […] Utilitymonster: That's exactly the right thing for HIC to do. Talk about lives saved with their public face, let hardcore members hear about x-risk, and then, in the future, if some excellent x-risk opportunity arises, direct resources to x-risk. - From felicia forum. References Gleiberman's paper: https://medialibrary.uantwerpen.be/files/8518/61565cb6-e056-4e35-bd2e-d14d58e35231.pdf Old EA wikipedia page (web archive): https://web.archive.org/web/20170409171350/https://en.wikipedia.org/wiki/Effective_altruism Old CEA webpage (web archive): https://web.archive.org/web/20161219031827/https://www.centreforeffectivealtruism.org/fundraising/ Socials Follow us on Twitter at @IncrementsPod, @BennyChugg, @VadenMasrani Come join our discord server! DM us on twitter or send us an email to get a supersecret link Become a patreon subscriber here. Or give us one-time cash donations to help cover our lack of cash donations here. Click dem like buttons on youtube Let us funnel you into the core group of super secret patreon supporters. Send us an email at incrementspodcast@gmail.com

Hic dies, et omnes dies futuri, mihi pertinent. Cognoscisne aliquem potentiorem quam Diabolus? Non puto te scire.We're playing Masks for this campaign! You can access a running list of all the NPCs from Campaign 4 here.Sponsors- United by Blue, creators of sustainable apparel and outdoor gear. Use code jointheparty for 20% off at https://unitedbyblue.comFind Us Online- website: https://jointhepartypod.com- patreon: https://patreon.com/jointhepartypod- instagram: https://instagram.com/jointhepartypod- twitter: https://twitter.com/jointhepartypod- tumblr: https://jointhepartypod.tumblr.com- facebook: https://facebook.com/jointhepartypod- merch & music: http://jointhepartypod.com/merchCast & Crew- Game Master, Co-Producer: Eric Silver- Co-Host, Co-Producer, Sound Designer, Composer (Connor Lyons): Brandon Grugle- Co-Host, Co-Producer, Editor (Shelley Craft): Julia Schifini- Co-Host, Co-Producer (Rowan Rosen): Amanda McLoughlin- Artwork: Allyson Wakeman- Multitude: https://multitude.productionsAbout UsJoin the Party is an actual play podcast with tangible worlds, genre-pushing storytelling, and collaborators who make each other laugh each week. We welcome everyone to the table, from longtime players to folks who've never touched a roleplaying game before. Hop into our current campaign: the drama and excitement of a superhero high school! Or marathon our completed stories: Campaign 3 for a pirate story set in a world of plant- and bug-folk, the Camp-Paign for a MOTW game set in a weird summer camp, Campaign 2 for a modern superhero game, and Campaign 1 for a high fantasy story. And once a month we release the Afterparty, where we answer your questions about the show and how we play the game. New episodes every Tuesday.See Privacy Policy at https://art19.com/privacy and California Privacy Notice at https://art19.com/privacy#do-not-sell-my-info.

 29 The next day, John saw Jesus coming to him, and he saith: Behold the Lamb of God, behold him who taketh away the sin of the world.Altera die vidit Joannes Jesum venientem ad se, et ait : Ecce agnus Dei, ecce qui tollit peccatum mundi. 30 This is he, of whom I said: After me there cometh a man, who is preferred before me: because he was before me.Hic est de quo dixi : Post me venit vir qui ante me factus est : quia prior me erat : 31 And I knew him not, but that he may be made manifest in Israel, therefore am I come baptizing with water.et ego nesciebam eum, sed ut manifestetur in Israel, propterea veni ego in aqua baptizans. 32 And John gave testimony, saying: I saw the Spirit coming down, as a dove from heaven, and he remained upon him.Et testimonium perhibuit Joannes, dicens : Quia vidi Spiritum descendentem quasi columbam de caelo, et mansit super eum. 33 And I knew him not; but he who sent me to baptize with water, said to me: He upon whom thou shalt see the Spirit descending, and remaining upon him, he it is that baptizeth with the Holy Ghost.Et ego nesciebam eum : sed qui misit me baptizare in aqua, ille mihi dixit : Super quem videris Spiritum descendentem, et manentem super eum, hic est qui baptizat in Spiritu Sancto. 34 And I saw, and I gave testimony, that this is the Son of God.Et ego vidi : et testimonium perhibui quia hic est Filius Dei.

Third Day in the Octave of Christmas19 And this he said, signifying by what death he should glorify God. And when he had said this, he saith to him: Follow me.Hoc autem dixit significans qua morte clarificaturus esset Deum. Et cum hoc dixisset, dicit ei : Sequere me. 20 Peter turning about, saw that disciple whom Jesus loved following, who also leaned on his breast at supper, and said: Lord, who is he that shall betray thee?Conversus Petrus vidit illum discipulum, quem diligebat Jesus, sequentem, qui et recubuit in coena super pectus ejus, et dixit : Domine, quis est qui tradet te? 21 Him therefore when Peter had seen, he saith to Jesus: Lord, and what shall this man do?Hunc ergo cum vidisset Petrus, dixit Jesu : Domine, hic autem quid? 22 Jesus saith to him: So I will have him to remain till I come, what is it to thee? follow thou me.Dicit ei Jesus : Sic eum volo manere donec veniam, quid ad te? tu me sequere. 23 This saying therefore went abroad among the brethren, that that disciple should not die. And Jesus did not say to him: He should not die; but, So I will have him to remain till I come, what is it to thee?Exiit ergo sermo iste inter fratres quia discipulus ille non moritur. Et non dixit ei Jesus : Non moritur, sed : Sic eum volo manere donec veniam, quid ad te? 24 This is that disciple who giveth testimony of these things, and hath written these things; and we know that his testimony is true.Hic est discipulus ille qui testimonium perhibet de his, et scripsit haec : et scimus quia verum est testimonium ejus.St John is the virgin Apostle, crowned with the halo of those who knew how to conquer their flesh; for this reason, he became "the disciple whom Jesus loved." Thanks to his angelic purity, he imbibed that wholesome wisdom of which that Epistle of which the Mass speaks and which has given to him the halo of the Doctors. Finally he received the halo of the Martyrs, since he barely escaped a violent death. It is to St John, who wrote a Gospel, three Epistles, and the Apocalypse, that we owe the most beautiful pages on the Divinity on the Word made flesh, and it is for this reason that the virgin Apostle is symbolised by the eagle. His name is mentioned with the other Apostles' names in the Canon of the Mass. St John the Evangelist departed this life at Ephesus. (101).

2 Now when John had heard in prison the works of Christ: sending two of his disciples he said to him:Joannes autem cum audisset in vinculis opera Christi, mittens duos de discipulis suis, 3 Art thou he that art to come, or look we for another?ait illi : Tu es, qui venturus es, an alium exspectamus? 4 And Jesus making answer said to them: Go and relate to John what you have heard and seen.Et respondens Jesus ait illis : Euntes renuntiate Joanni quae audistis, et vidistis. 5 The blind see, the lame walk, the lepers are cleansed, the deaf hear, the dead rise again, the poor have the gospel preached to them.Caeci vident, claudi ambulant, leprosi mundantur, surdi audiunt, mortui resurgunt, pauperes evangelizantur : 6 And blessed is he that shall not be scandalized in me.et beatus est, qui non fuerit scandalizatus in me. 7 And when they went their way, Jesus began to say to the multitudes concerning John: What went you out into the desert to see? a reed shaken with the wind?Illis autem abeuntibus, coepit Jesus dicere ad turbas de Joanne : Quid existis in desertum videre? arundinem vento agitatem? 8 But what went you out to see? a man clothed in soft garments? Behold they that are clothed in soft garments, are in the houses of kings.Sed quid existis videre? hominem mollibus vestitum? Ecce qui mollibus vestiuntur, in domibus regum sunt. 9 But what went you out to see? a prophet? yea I tell you, and more than a prophet.Sed quid existis videre? prophetam? Etiam dico vobis, et plus quam prophetam. 10 For this is he of whom it is written: Behold I send my angel before thy face, who shall prepare thy way before thee.Hic est enim de quo scriptum est : Ecce ego mitto angelum meum ante faciem tuam, qui praeparabit viam tuam ante te

 2 Now when John had heard in prison the works of Christ: sending two of his disciples he said to him:Joannes autem cum audisset in vinculis opera Christi, mittens duos de discipulis suis, 3 Art thou he that art to come, or look we for another?ait illi : Tu es, qui venturus es, an alium exspectamus? 4 And Jesus making answer said to them: Go and relate to John what you have heard and seen.Et respondens Jesus ait illis : Euntes renuntiate Joanni quae audistis, et vidistis. 5 The blind see, the lame walk, the lepers are cleansed, the deaf hear, the dead rise again, the poor have the gospel preached to them.Caeci vident, claudi ambulant, leprosi mundantur, surdi audiunt, mortui resurgunt, pauperes evangelizantur : 6 And blessed is he that shall not be scandalized in me.et beatus est, qui non fuerit scandalizatus in me. 7 And when they went their way, Jesus began to say to the multitudes concerning John: What went you out into the desert to see? a reed shaken with the wind?Illis autem abeuntibus, coepit Jesus dicere ad turbas de Joanne : Quid existis in desertum videre? arundinem vento agitatem? 8 But what went you out to see? a man clothed in soft garments? Behold they that are clothed in soft garments, are in the houses of kings.Sed quid existis videre? hominem mollibus vestitum? Ecce qui mollibus vestiuntur, in domibus regum sunt. 9 But what went you out to see? a prophet? yea I tell you, and more than a prophet.Sed quid existis videre? prophetam? Etiam dico vobis, et plus quam prophetam. 10 For this is he of whom it is written: Behold I send my angel before thy face, who shall prepare thy way before thee.Hic est enim de quo scriptum est : Ecce ego mitto angelum meum ante faciem tuam, qui praeparabit viam tuam ante te.

 2 Now when John had heard in prison the works of Christ: sending two of his disciples he said to him:Joannes autem cum audisset in vinculis opera Christi, mittens duos de discipulis suis, 3 Art thou he that art to come, or look we for another?ait illi : Tu es, qui venturus es, an alium exspectamus? 4 And Jesus making answer said to them: Go and relate to John what you have heard and seen.Et respondens Jesus ait illis : Euntes renuntiate Joanni quae audistis, et vidistis. 5 The blind see, the lame walk, the lepers are cleansed, the deaf hear, the dead rise again, the poor have the gospel preached to them.Caeci vident, claudi ambulant, leprosi mundantur, surdi audiunt, mortui resurgunt, pauperes evangelizantur : 6 And blessed is he that shall not be scandalized in me.et beatus est, qui non fuerit scandalizatus in me. 7 And when they went their way, Jesus began to say to the multitudes concerning John: What went you out into the desert to see? a reed shaken with the wind?Illis autem abeuntibus, coepit Jesus dicere ad turbas de Joanne : Quid existis in desertum videre? arundinem vento agitatem? 8 But what went you out to see? a man clothed in soft garments? Behold they that are clothed in soft garments, are in the houses of kings.Sed quid existis videre? hominem mollibus vestitum? Ecce qui mollibus vestiuntur, in domibus regum sunt. 9 But what went you out to see? a prophet? yea I tell you, and more than a prophet.Sed quid existis videre? prophetam? Etiam dico vobis, et plus quam prophetam. 10 For this is he of whom it is written: Behold I send my angel before thy face, who shall prepare thy way before thee.Hic est enim de quo scriptum est : Ecce ego mitto angelum meum ante faciem tuam, qui praeparabit viam tuam ante te.

The DanielC Radio Show this month opens with DanielC's new remix of Moon Bee - what a year?, setting the tone for a journey built around artists who bring their own identity to every track. It moves into fresh indie cuts from Maryn Charlie, One Nice Boi, Bless You, and others, each adding its own colour to the mix. The show then shifts into darker Bass House with Xpilot, Migos, Not Yurs, R3HAB, and Vion Konger. A nostalgic wave follows with new music from Noacht1, HI-CØ, and Barcelona duo Panpe n'u, all carrying their own creative fingerprint. The energy rises into Raw Bass House from Tempered DJs, Butter, Reeva, and more. This month's show closes with euphoric Progressive House from Jack Shore & Dodo Nyoka, Almero, Paul Green & Fred Chase, and a renewed take on Walking On A Dream by Axwell and Michael Calfan. DanielC Radio Show #008 shines a light on artists carving out their own sound and the talent pushing this scene forward.

David Waldman and Greg Dworkin help us start the month, week and day off right. You read it here first!  (Unless you read it over at The Intercept a few hours earlier.) The United States… that's us... We blew up a boat with 11 people in it, then we circled around and we murdered all the survivors.  We have murdered at least 83 in boats… Most of the world understands that is wrong, and we might have stopped murdering the swimmers, which we might all agree is somehow even more reprehensible. But we didn't just decide that was bad yesterday, we always knew that was a bad thing to do. Except of course for Pete Hegseth, who we, or at least some of us, hired to not do an evil job. Hic-seth thought that this was really funny at first but sobered up quickly to transfer the blame to his soldiers. Donald K. Trump doesn't know a "Hegseth", but if you write it at the bottom of the check, he'll pardon him. Juan Orlando Hernández made millions as president, raping Honduras in partnership with El Chapo while creating one of the largest drug-trafficking organizations in the world and was sentenced to 45 years. Oh well. Alina Habba, one of the biggest Trump's lawyers, is out as U.S. Attorney for the District of New Jersey. Sure, Kristi Noem disregarded court orders to send Venezuelans to El Salvador, but that wouldn't be against the law if judges didn't say so. No one needs more ballroom less than Trump and even his architect says so. Do reactionaries just feel left out? Trump has almost reached Jan 6 levels of unpopularity, and Republicans are feeling it. Another Gop seat could be flipped in Tennessee tomorrow, as they plan to redistrict Nashville away. One Republican Senator in Indiana drew the line on Trump insulting his daughter, while another wasn't swayed by swatting and doxxing. and yet another by a pipe bomb. Meanwhile, Troy Nehls of Texas feels now should be when he spends more time with his family.

In this episode of the Epigenetics Podcast, we talked with Anders Sejr Hansen from MIT about his work on the impact of 3D genome structures on gene expression, the roles of proteins like CTCF and cohesin, and advanced techniques like Region Capture Micro-C for mapping genome organisation. Dr. Sejr Hansen introduces his research focusing on the relationship between three-dimensional genome structure and function, specifically how these structures can influence gene expression. He elaborates on the importance of transcription factors and the role of looping structures in gene regulation, emphasizing the implications of his work for understanding gene functionality in the context of both development and disease. The conversation then shifts to discussing loop extrusion and the factors affecting loop stability, primarily CTCF and cohesin. Dr. Sejr Hansen highlights the dynamics of these proteins' binding interactions and how their speeds challenge the notion of stable looping structures in the genome. With a keen interest in CTCF's role, he explains how the protein interacts with DNA and the mechanistic aspects of transcription factor movement, alluding to research findings that reveal that CTCF and cohesin tend to form clusters which may play vital roles in establishing chromatin structure. As the interview progresses, Dr. Sejr Hansen details his transition to leading his own lab at MIT, emphasizing the continuation of his earlier work while expanding into new methodologies for studying chromatin. He underscores the importance of understanding not just the static structures of DNA interactions, but the dynamic nature of these relationships and how they influence gene expression. His lab's recent focus has included using advanced imaging techniques to assess the dynamics of chromatin interactions more precisely. The discussion then touches on specific findings from Dr. Sejr Hansen's lab regarding the relationship between genome organization and double-strand break repair mechanisms. He emphasizes how the repair machinery can affect chromatin structure and underscores the essential role of cohesin in facilitating effective double-strand break repair by keeping broken DNA ends in proximity. He suggests that loop extrusion might help prevent genetic material from diffusing too far apart and improve the efficiency of repair. Dr. Sejr Hansen also discusses innovations in genome mapping techniques, particularly the development of Region Capture Micro-C, which facilitates deeper insights into the three-dimensional organization of the genome. This method allows researchers to achieve significantly higher resolution in their analyses compared to traditional 3D genomics techniques like Hi-C. He outlines the technical process and the implications of their findings, especially regarding enhancer-promoter interactions and the surprisingly promiscuous nature of these relationships. References Anders S Hansen, Iryna Pustova, Claudia Cattoglio, Robert Tjian, Xavier Darzacq (2017) CTCF and cohesin regulate chromatin loop stability with distinct dynamics eLife 6:e25776 https://doi.org/10.7554/eLife.25776 Claudia Cattoglio, Iryna Pustova, Nike Walther, Jaclyn J Ho, Merle Hantsche-Grininger, Carla J Inouye, M Julius Hossain, Gina M Dailey, Jan Ellenberg, Xavier Darzacq, Robert Tjian, Anders S Hansen (2019) Determining cellular CTCF and cohesin abundances to constrain 3D genome models eLife 8:e40164 https://doi.org/10.7554/eLife.40164 Goel, V.Y., Huseyin, M.K. & Hansen, A.S. Region Capture Micro-C reveals coalescence of enhancers and promoters into nested microcompartments. Nat Genet 55, 1048–1056 (2023). https://doi.org/10.1038/s41588-023-01391-1 Related Episodes Biophysical Modeling of 3-D Genome Organization (Leonid Mirny) Unraveling Mechanisms of Chromosome Formation (Job Dekker) Contact Epigenetics Podcast on Mastodon Epigenetics Podcast on Bluesky Dr. Stefan Dillinger on LinkedIn Active Motif on LinkedIn Active Motif on Bluesky Email: podcast@activemotif.com

1 And entering into a boat, he passed over the water and came into his own city.Et ascendens in naviculam, transfretavit, et venit in civitatem suam. 2 And behold they brought to him one sick of the palsy lying in a bed. And Jesus, seeing their faith, said to the man sick of the palsy: Be of good heart, son, thy sins are forgiven thee.Et ecce offerebant ei paralyticum jacentem in lecto. Et videns Jesus fidem illorum, dixit paralytico : Confide fili, remittuntur tibi peccata tua. 3 And behold some of the scribes said within themselves: He blasphemeth.Et ecce quidam de scribis dixerunt intra se : Hic blasphemat. 4 And Jesus seeing their thoughts, said: Why do you think evil in your hearts?Et cum vidisset Jesus cogitationes eorum, dixit : Ut quid cogitatis mala in cordibus vestris? 5 Whether is easier, to say, Thy sins are forgiven thee: or to say, Arise, and walk?Quid est facilius dicere : Dimittuntur tibi peccata tua : an dicere : Surge, et ambula? 6 But that you may know that the Son of man hath power on earth to forgive sins, (then said he to the man sick of palsy,) Arise, take up thy bed, and go into thy house.Ut autem sciatis, quia Filius hominis habet potestatem in terra dimittendi peccata, tunc ait paralytico : Surge, tolle lectum tuum, et vade in domum tuam. 7 And he arose, and went into his house.Et surrexit, et abiit in domum suam. 8 And the multitude seeing it, feared, and glorified God that gave such power to men.Videntes autem turbae timuerunt, et glorificaverunt Deum, qui dedit potestatem talem hominibus.Healing of the sick of the palsy in Capernaum. The Priests have received power to heal souls, to pardon sinners.

Ever steal your mom's SnackWells? Were Fruit Gushers a total scam? Was Trix yogurt ACTUALLY healthy?? Cody, Andrew, & Ren breakdown greatest school lunch and after school food items INCLUDING The Hierarchy of Lunchables! Feminist Icon, Little Debbie! Fruit Roll-ups VS Fruit by the Foot! Eggo Waffles vs Pop Tarts! Push Pops vs Ring pops! Hi-C vs SunnyD! Totino's! Bagel Bites! Hot Pockets! How Cody's political advocacy got him a brick of Toaster Strudel frosting! AND MUCH MORE!We have fall deals for YOU!!Hims: Need help with E.D.? Get simple, online access to personalized, affordable care at Hims.com/pettyQuince: Layer up this fall! Go to Quince.com/pettiness for free shipping on your order and 365-day returns!LMNT: Stay replenished! Get your free 8-count Sample Pack of LMNT's most popular drink mix flavors with any purchase at DrinkLMNT.com/tactfulSee Privacy Policy at https://art19.com/privacy and California Privacy Notice at https://art19.com/privacy#do-not-sell-my-info.

Recently published research by Prof. Wrigley-Field and her colleagues found that between 1980 and 2023 the total number of US excess deaths equaled 14.7 million. Between 2010 and 2023 excess deaths ranged between 120% and 130% higher compared to other HIC (High Income Countries). Possibly more disturbing the authors found US excess deaths were moreover among working-age adults, for example, in 2023 excess deaths among US adults aged 25-44 were 2.6 times higher than in other HIC. That same year excess deaths remarkably made up almost 23% of all deaths and 46% of excess deaths were among people younger than 65 years. The causes of excess deaths since 1980 have on balance largely been the result of preventable cardiometabolic causes and drug overdoses. About these findings, Prof Wrigley-Field's coauthor, Prof. Jacob Bor, commented, “if the US simply performed at the average of our peers, one out of every two US deaths under 65 years is likely avoidable. Our failure to address this is a national scandal.” Prof. Wrigley-Fields writings discussed during this podcast are at: https://jamanetwork.com/journals/jama-health-forum/fullarticle/2834281https://jamanetwork.com/journals/jamanetworkopen/fullarticle/2829783https://www.annualreviews.org/content/journals/10.1146/annurev-soc-031021-105213 This is a public episode. If you would like to discuss this with other subscribers or get access to bonus episodes, visit www.thehealthcarepolicypodcast.com

36 And one of the Pharisees desired him to eat with him. And he went into the house of the Pharisee, and sat down to meat.Rogabat autem illum quidam de pharisaeis ut manducaret cum illo. Et ingressus domum pharisaei discubuit. 37 And behold a woman that was in the city, a sinner, when she knew that he sat at meat in the Pharisee's house, brought an alabaster box of ointment;Et ecce mulier, quae erat in civitate peccatrix, ut cognovit quod accubuisset in domo pharisaei, attulit alabastrum unguenti : 38 And standing behind at his feet, she began to wash his feet, with tears, and wiped them with the hairs of her head, and kissed his feet, and anointed them with the ointment.et stans retro secus pedes ejus, lacrimis coepit rigare pedes ejus, et capillis capitis sui tergebat, et osculabatur pedes ejus, et unguento ungebat. 39 And the Pharisee, who had invited him, seeing it, spoke within himself, saying: This man, if he were a prophet, would know surely who and what manner of woman this is that toucheth him, that she is a sinner.Videns autem pharisaeus, qui vocaverat eum, ait intra se dicens : Hic si esset propheta, sciret utique quae et qualis est mulier, quae tangit eum : quia peccatrix est. 40 And Jesus answering, said to him: Simon, I have somewhat to say to thee. But he said: Master, say it.Et respondens Jesus, dixit ad illum : Simon, habeo tibi aliquid dicere. At ille ait : Magister, dic. 41 A certain creditor had two debtors, the one who owed five hundred pence, and the other fifty.Duo debitores erant cuidam foeneratori : unus debebat denarios quingentos, et alius quinquaginta. 42 And whereas they had not wherewith to pay, he forgave them both. Which therefore of the two loveth him most?Non habentibus illis unde redderent, donavit utrisque. Quis ergo eum plus diligit? 43 Simon answering, said: I suppose that he to whom he forgave most. And he said to him: Thou hast judged rightly.Respondens Simon dixit : Aestimo quia is cui plus donavit. At ille dixit : Recte judicasti. 44 And turning to the woman, he said unto Simon: Dost thou see this woman? I entered into thy house, thou gavest me no water for my feet; but she with tears hath washed my feet, and with her hairs hath wiped them.Et conversus ad mulierem, dixit Simoni : Vides hanc mulierem? Intravi in domum tuam, aquam pedibus meis non dedisti : haec autem lacrimis rigavit pedes meos, et capillis suis tersit. 45 Thou gavest me no kiss; but she, since she came in, hath not ceased to kiss my feet.Osculum mihi non dedisti : haec autem ex quo intravit, non cessavit osculari pedes meos. 46 My head with oil thou didst not anoint; but she with ointment hath anointed my feet.Oleo caput meum non unxisti : haec autem unguento unxit pedes meos. 47 Wherefore I say to thee: Many sins are forgiven her, because she hath loved much. But to whom less is forgiven, he loveth less.Propter quod dico tibi : remittuntur ei peccata multa, quoniam dilexit multum. Cui autem minus dimittitur, minus diligit. 48 And he said to her: Thy sins are forgiven thee.Dixit autem ad illam : Remittuntur tibi peccata. 49 And they that sat at meat with him began to say within themselves: Who is this that forgiveth sins also?Et coeperunt qui simul accumbebant, dicere intra se : Quis est hic qui etiam peccata dimittit? 50 And he said to the woman: Thy faith hath made thee safe, go in peace.Dixit autem ad mulierem : Fides tua te salvam fecit : vade in pace.Let us weep for our sins.

Osteosarcoma Webinar Series: Yanding Zhao, PhD to discuss how Distinct patterns of chromosomal instability fuel osteosarcoma progression and influence patient outcomes.Osteosarcoma (OS) is notable for extreme chromosomal instability (CIN) and molecular heterogeneity, which have hindered therapeutic progress. To address this, the lab performed longitudinal and multi-modal profiling of 91 tumors from 71 pediatric patients, integrating whole-genome and transcriptome sequencing with ATAC-seq and Hi-C in matched cell lines. Their analyses revealed that key driver mutations, including TP53, are fixed early and persist through progression. Over 80% of tumors exhibited complex structural alterations—such as chromothripsis, kataegis, loss of heterozygosity, and ecDNA amplification—with MYC enhancer hijacking linked to chemoresistance. They identified a high-risk evolutionary trajectory marked by homologous recombination deficiency (HRD)-like signatures in the absence of BRCA mutations. These tumors showed focal duplications at fragile sites, early whole-genome doubling, high TP53 mutation burden, and sensitivity to PARP inhibition—highlighting a potential therapeutic vulnerability. Together, these findings define a replication stress–driven model of OS evolution, shaped by early chromosomal remodeling and ecDNA-mediated oncogene activation, with implications for biomarker development and precision treatment strategies.Dr. Yanding Zhao is a postdoctoral researcher at Stanford University in the lab of Dr. Christina Curtis. He earned his PhD in Genetics from Dartmouth College, where he began developing computational tools to understand how genome instability disrupts gene regulation in cancer. At Stanford, his research focuses on pediatric osteosarcoma. By combining genome sequencing, 3D chromatin mapping, and spatial transcriptomics, he studies how tumors evolve, resist treatment, and evade the immune system. Dr. Zhao works closely with clinicians and scientists to help turn these discoveries into potential new therapies. He is honored to be part of the MIB Agents community and looks forward to sharing his work in a way that resonates with patients, families, and advocates.

As part of the US HUPO sponsored "Alt Proteomics" series highlighting alternative proteomics things, Ben and Ben sit down to talk with Dr. Olga Dudchenko, Baylor College of Medicine.keywords: structural genomics; Hi-C; DNA Zoo

In this episode, Marcela Collier talks with Christina, a parent who transformed her parenting approach through HIC coaching. Discover how Christina overcame reactivity and anxiety to foster a secure and calm environment for her child. If you want to stop yelling and reacting in anger, and instead become a calm, confident parent who knows exactly how to respond during your child's chaos… join our FREE class: The Parenting With Understanding™ System of Needs.In this class, you'll learn: ✅ How to stop angry reactions and respond with calm.✅ How to get your kids to listen even when you say "no."✅ How to raise emotionally healthy kids who feel safe and loved.

No episódio de hoje do Check-up Semanal, o Dr. Ronaldo Gismondi, diretor médico da Afya e editor-chefe médico do Portal Afya e do Whitebook, apresenta os principais destaques do mês na área de Terapia Intensiva, com foco em evidências clínicas e diretrizes atualizadas.O que você vai ouvir neste episódio:- Vasopressina precoce no choque sépticoEstudo observacional (OVISS) mostra que iniciar vasopressina mais cedo e com menor dose de norepinefrina reduz mortalidade hospitalar e necessidade de diálise.- Traqueostomia precoce em pacientes críticosRevisão de ensaios clínicos sugere que realizar a TQT entre o 7º e 10º dia diminui a incidência de pneumonia associada à ventilação (PAV), ainda que não reduza mortalidade.- Extubação em vias aéreas difíceisEstratégias para reduzir risco de reintubação, com uso do escore REVERSE e cuff leak test para avaliação pré-extubação.- Hemorragia intracerebral (HIC): tempo é cérebroProtocolo Code-ICH propõe metas de tempo e controle rigoroso de pressão arterial, glicemia e temperatura para limitar expansão do hematoma.- Estudo OPTPRESS: alvo pressórico ideal em idosos sépticosPAM mais elevada (80–85 mmHg) aumenta mortalidade e eventos adversos em comparação com o alvo tradicional de 65–70 mmHg, mesmo em pacientes hipertensos.Aperte o play e mantenha-se atualizado com as principais evidências que impactam a prática em terapia intensiva!#TerapiaIntensiva #CheckupSemanal #ChoqueSéptico #Vasopressina #Traqueostomia #HemorragiaIntracraniana #UTI #PortalAfya #AtualizaçãoMédica

 1 And after six days Jesus taketh unto him Peter and James, and John his brother, and bringeth them up into a high mountain apart:Et post dies sex assumit Jesus Petrum, et Jacobum, et Joannem fratrem ejus, et ducit illos in montem excelsum seorsum : 2 And he was transfigured before them. And his face did shine as the sun: and his garments became white as snow.et transfiguratus est ante eos. Et resplenduit facies ejus sicut sol : vestimenta autem ejus facta sunt alba sicut nix. 3 And behold there appeared to them Moses and Elias talking with him.Et ecce apparuerunt illis Moyses et Elias cum eo loquentes. 4 And Peter answering, said to Jesus: Lord, it is good for us to be here: if thou wilt, let us make here three tabernacles, one for thee, and one for Moses, and one for Elias.Respondens autem Petrus, dixit ad Jesum : Domine, bonum est nos hic esse : si vis, faciamus tria tabernacula, tibi unum, Moysi unum, et Eliae unum. 5 And as he was yet speaking, behold a bright cloud overshadowed them. And lo, a voice out of the cloud, saying: This is my beloved Son, in whom I am well pleased: hear ye him.Adhuc eo loquente, ecce nubes lucida obumbravit eos. Et ecce vox de nube, dicens : Hic est Filius meus dilectus, in quo mihi bene complacui : ipsum audite. 6 And the disciples hearing, fell upon their face, and were very much afraid.Et audientes discipuli ceciderunt in faciem suam, et timuerunt valde. 7 And Jesus came and touched them: and said to them, Arise, and fear not.Et accessit Jesus, et tetigit eos : dixitque eis : Surgite, et nolite timere. 8 And they lifting up their eyes saw no one but only Jesus.Levantes autem oculos suos, neminem viderunt, nisi solum Jesum. 9 And as they came down from the mountain, Jesus charged them, saying: Tell the vision to no man, till the Son of man be risen from the dead.Et descendentibus illis de monte, praecepit eis Jesus, dicens : Nemini dixeritis visionem, donec Filius hominis a mortuis resurgat.

 36 And one of the Pharisees desired him to eat with him. And he went into the house of the Pharisee, and sat down to meat.Rogabat autem illum quidam de pharisaeis ut manducaret cum illo. Et ingressus domum pharisaei discubuit. 37 And behold a woman that was in the city, a sinner, when she knew that he sat at meat in the Pharisee's house, brought an alabaster box of ointment;Et ecce mulier, quae erat in civitate peccatrix, ut cognovit quod accubuisset in domo pharisaei, attulit alabastrum unguenti : 38 And standing behind at his feet, she began to wash his feet, with tears, and wiped them with the hairs of her head, and kissed his feet, and anointed them with the ointment.et stans retro secus pedes ejus, lacrimis coepit rigare pedes ejus, et capillis capitis sui tergebat, et osculabatur pedes ejus, et unguento ungebat. 39 And the Pharisee, who had invited him, seeing it, spoke within himself, saying: This man, if he were a prophet, would know surely who and what manner of woman this is that toucheth him, that she is a sinner.Videns autem pharisaeus, qui vocaverat eum, ait intra se dicens : Hic si esset propheta, sciret utique quae et qualis est mulier, quae tangit eum : quia peccatrix est. 40 And Jesus answering, said to him: Simon, I have somewhat to say to thee. But he said: Master, say it.Et respondens Jesus, dixit ad illum : Simon, habeo tibi aliquid dicere. At ille ait : Magister, dic. 41 A certain creditor had two debtors, the one who owed five hundred pence, and the other fifty.Duo debitores erant cuidam foeneratori : unus debebat denarios quingentos, et alius quinquaginta. 42 And whereas they had not wherewith to pay, he forgave them both. Which therefore of the two loveth him most?Non habentibus illis unde redderent, donavit utrisque. Quis ergo eum plus diligit? 43 Simon answering, said: I suppose that he to whom he forgave most. And he said to him: Thou hast judged rightly.Respondens Simon dixit : Aestimo quia is cui plus donavit. At ille dixit : Recte judicasti. 44 And turning to the woman, he said unto Simon: Dost thou see this woman? I entered into thy house, thou gavest me no water for my feet; but she with tears hath washed my feet, and with her hairs hath wiped them.Et conversus ad mulierem, dixit Simoni : Vides hanc mulierem? Intravi in domum tuam, aquam pedibus meis non dedisti : haec autem lacrimis rigavit pedes meos, et capillis suis tersit. 45 Thou gavest me no kiss; but she, since she came in, hath not ceased to kiss my feet.Osculum mihi non dedisti : haec autem ex quo intravit, non cessavit osculari pedes meos. 46 My head with oil thou didst not anoint; but she with ointment hath anointed my feet.Oleo caput meum non unxisti : haec autem unguento unxit pedes meos. 47 Wherefore I say to thee: Many sins are forgiven her, because she hath loved much. But to whom less is forgiven, he loveth less.Propter quod dico tibi : remittuntur ei peccata multa, quoniam dilexit multum. Cui autem minus dimittitur, minus diligit. 48 And he said to her: Thy sins are forgiven thee.Dixit autem ad illam : Remittuntur tibi peccata. 49 And they that sat at meat with him began to say within themselves: Who is this that forgiveth sins also?Et coeperunt qui simul accumbebant, dicere intra se : Quis est hic qui etiam peccata dimittit? 50 And he said to the woman: Thy faith hath made thee safe, go in peace.Dixit autem ad mulierem : Fides tua te salvam fecit : vade in pace.St Mary Magdalen, of Magdala in Galilee, was the sister of St Martha and St Lazarus. First a sinner, she was converted by our Lord, who raised Lazarus at her prayer. She stood at the Cross "till our Lord sent forth His Spirit", After His Victory, Christ showed Himself to Magdalen and made her his messenger to announce His Resurrection to the Apostles.

Monica Guerzoni racconta la prima giornata della conferenza per la ricostruzione dell'Ucraina, a Roma. Virginia Piccolillo spiega gli sviluppi sul caso del generale libico liberato dall'Italia che sta creando problemi al governo e al ministro della Giustizia. E Leonard Berberi parla della novità introdotta dall'Enac per cui negli aeroporti, all'imbarco, non sarà più chiesta la carta d'identità.I link di corriere.it:Von der Leyen alla Conferenza di Roma lancia il Fondo azionario Ue per la ricostruzione dell'Ucraina. Mattarella: «Kiev può contare sul nostro sostegno»Caso Almasri, Nordio: «Io dimissionario? Hic manebimus optime. E gli atti smentiscono i giornali». Anche Bartolozzi restaPer volare in Italia e in Europa non serve più mostrare la carta d'identità: ecco cosa cambia

In this insightful episode of Parallax, Dr Ankur Kalra explores the emerging field of cardiac epigenetics with Dr Manuel Rosa-Garrido, assistant professor of medicine at the University of Alabama in Birmingham. Their conversation examines how the three-dimensional organization of DNA within the cell nucleus influences heart disease development beyond traditional genetic sequencing. Dr Rosa-Garrido shares his pioneering work using Hi-C technology to map genome structure, revealing that DNA arrangement plays a crucial role in cardiac pathology. The discussion covers key concepts including chromatin loops, topologically associating domains, and compartmentalization—explaining how these structures regulate gene expression and how their disruption contributes to cardiovascular disease. The episode explores practical implications for clinical practice, from early biomarker identification to potential CRISPR-based therapies that could target chromatin structure. Dr Rosa-Garrido outlines how this research could transform cardiovascular care within the next decade, offering new approaches to both inherited cardiomyopathies and environmentally-influenced conditions like atherosclerosis. Valuable insights for cardiologists interested in precision medicine and the evolving understanding of how genetic architecture influences cardiovascular risk and treatment strategies. Questions and comments can be sent to "podcast@radcliffe-group.com" and may be answered by Ankur in the next episode. Host: @AnkurKalraMD and produced by: @RadcliffeCardio Parallax is Ranked in the Top 100 Health Science Podcasts (#48) by Million Podcasts.

On today's MJ Morning Show: Poop cruise documentary Morons in the news Walmart self-checkout user called police on himself Lays has new potato chip flavor "Harmless" comments that may trigger people Track runner has uniform malfunction Bezos wedding update MJ's license issues continue MJ Instagram F1 Thrift Shop visits 5 stages of love Secret to marriage New sweeper Door plug on a 737 that flew off... Someone was on vacation Is it ok to eat your lunch at a cemetery? Food tourism ChatGPT is just plain wrong Millennials are cutting back Billionaire heir car crash Vaping news... worse than you realize Gen Z girls are wearing camo Disney is planning something big Woman used a product to look younger... made her look much older Hi C... explodes?

In October 2024, Prabowo Subianto became president of Indonesia. He inherits the “Golden Indonesia” vision: By the time the country celebrates 100 years of independence in 2045, it aims to be one of the five largest economies in the world. But if Indonesia remains dependent on commodity exports like palm oil, coal, natural gas, and rubber, does it risk getting stuck in the “middle income trap” – too wealthy to compete with low-wage nations, but without the human capital or technology to become a HIC? Chatib Basri is an economist and former finance minister of Indonesia. He tells Tim Phillips about the industrial policies needed to accelerate Indonesia's economy and diversify its exports, and the challenges if Indonesia does not accelerate its growth. Read the full show notes on VoxDev: https://voxdev.org/topic/macroeconomics-growth/going-economic-growth-lessons-indonesia Also on VoxDev: Is improving tax administration more effective than raising tax rates?  https://voxdev.org/topic/public-economics/improving-tax-administration-more-effective-raising-tax-rates-evidence

56 For my flesh is meat indeed: and my blood is drink indeed.Caro enim mea vere est cibus : et sanguis meus, vere est potus; 57 He that eateth my flesh, and drinketh my blood, abideth in me, and I in him.qui manducat meam carnem et bibit meum sanguinem, in me manet, et ego in illo. 58 As the living Father hath sent me, and I live by the Father; so he that eateth me, the same also shall live by me.Sicut misit me vivens Pater, et ego vivo propter Patrem : et qui manducat me, et ipse vivet propter me. 59 This is the bread that came down from heaven. Not as your fathers did eat manna, and are dead. He that eateth this bread, shall live for ever.Hic est panis qui de caelo descendit. Non sicut manducaverunt patres vestri manna, et mortui sunt. Qui manducat hunc panem, vivet in aeternum.The Eucharist is instituted in the form of food, so that we may receive in Holy Communion the Victim of the Cross.

44 No man can come to me, except the Father, who hath sent me, draw him; and I will raise him up in the last day.nemo potest venire ad me, nisi Pater, qui misit me, traxerit eum; et ego resuscitabo eum in novissimo die. 45 It is written in the prophets: And they shall all be taught of God. Every one that hath heard of the Father, and hath learned, cometh to me.Est scriptum in prophetis : Et erunt omnes docibiles Dei. Omnis qui audivit a Patre, et didicit, venit ad me. 46 Not that any man hath seen the Father; but he who is of God, he hath seen the Father.Non quia Patrem vidit quisquam, nisi is, qui est a Deo, hic vidit Patrem. 47 Amen, amen I say unto you: He that believeth in me, hath everlasting life.Amen, amen dico vobis : qui credit in me, habet vitam aeternam. 48 I am the bread of life.Ego sum panis vitae. 49 Your fathers did eat manna in the desert, and are dead.Patres vestri manducaverunt manna in deserto, et mortui sunt. 50 This is the bread which cometh down from heaven; that if any man eat of it, he may not die.Hic est panis de caelo descendens : ut si quis ex ipso manducaverit, non moriatur. 51 I am the living bread which came down from heaven.Ego sum panis vivus, qui de caelo descendi. 52 If any man eat of this bread, he shall live for ever; and the bread that I will give, is my flesh, for the life of the world.Si quis manducaverit ex hoc pane, vivet in aeternum : et panis quem ego dabo, caro mea est pro mundi vita.

Sarah Wreath is building something solid. After turning heads with her contribution to Sandwell District's End Beginnings, she steps forward with 光と影 (Hikari to Kage), a new EP on a promising Dutch label Wabi Sabi Audio Imprint. It's a compact, carefully crafted release featuring two originals accompanied by remixes from Pianeti Sintetici, HI-C, and label founder Jorge Fons. If you're into vinyl and care about detail, this one is for the shelf. The title track opens with restraint: low-end pressure, drawn-out pads, and a pulse that feels lived-in. Over time, acid stabs and spacey synths slip in, not to explode, but to shift the atmosphere. There's a suggestion of movement in the title, but the track doesn't chase light or fall into darkness. It stays in the grey, in the tension. It's subtle, textural, and confident in its stillness.  光と影 (Hikari to Kage) releases June 6 via Wabi Sabi's Bandcamp. https://soundcloud.com/sarahwreath https://soundcloud.com/wabisabi_ai https://www.instagram.com/sarahhwreath/ https://www.instagram.com/wabisabi__ai Follow us on social media: https://soundcloud.com/itsdelayed https://linktr.ee/delayed https://www.delayed.nyc https://www.facebook.com/itsdelayed https://www.instagram.com/_____delayed https://www.youtube.com/@_____delayed Contact us: info@delayed.nyc

46 He came again therefore into Cana of Galilee, where he made the water wine. And there was a certain ruler, whose son was sick at Capharnaum.Venit ergo iterum in Cana Galilaeae, ubi fecit aquam vinum. Et erat quidam regulus, cujus filius infirmabatur Capharnaum. 47 He having heard that Jesus was come from Judea into Galilee, went to him, and prayed him to come down, and heal his son; for he was at the point of death.Hic cum audisset quia Jesus adveniret a Judaea in Galilaeam, abiit ad eum, et rogabat eum ut descenderet, et sanaret filium ejus : incipiebat enim mori. 48 Jesus therefore said to him: Unless you see signs and wonders, you believe not.Dixit ergo Jesus ad eum : Nisi signa et prodigia videritis, non creditis. 49 The ruler saith to him: Lord, come down before that my son die.Dicit ad eum regulus : Domine, descende priusquam moriatur filius meus. 50 Jesus saith to him: Go thy way; thy son liveth. The man believed the word which Jesus said to him, and went his way.Dicit ei Jesus : Vade, filius tuus vivit. Credidit homo sermoni quem dixit ei Jesus, et ibat. 51 And as he was going down, his servants met him; and they brought word, saying, that his son lived.Jam autem eo descendente, servi occurrerunt ei, et nuntiaverunt dicentes, quia filius ejus viveret. 52 He asked therefore of them the hour wherein he grew better. And they said to him: Yesterday, at the seventh hour, the fever left him.Interrogabat ergo horam ab eis in qua melius habuerit. Et dixerunt ei : Quia heri hora septima reliquit eum febris. 53 The father therefore knew, that it was at the same hour that Jesus said to him, Thy son liveth; and himself believed, and his whole house.Cognovit ergo pater, quia illa hora erat in qua dixit ei Jesus : Filius tuus vivit; et credidit ipse et domus ejus tota.Nereus and Achilleus were servants of Flavius Domitilla, who was martyred with them A.D. 98. St Pancras was put to death at the age of fourteen. A.D. 304

Janneta Tabakov, Enterprise Growth Lead with Perplexity who focuses on AI in biotech and biopharma speaks with Pitt HexAI Podcast host Jordan Gass-Pooré about her work at Perplexity, a popular and fast-growing new AI-powered answer engine that's redefining search and information discovery in the AI era. Janneta and Jordan discuss how Perplexity (https://www.perplexity.ai/) makes information discovery clear, trustworthy, and accessible, especially in fields like health, medicine, and science and they touch on Janneta's prior work at Arima Genomics, the field of 3D genomics, Hi-C technology and the visualization and mapping of DNA structures.The episode is geared towards introducing students and healthcare professionals to new AI-powered search tools and information discovery processes. The episode highlights how tools like Perplexity are advancing explainable AI in information discovery by making the search and synthesis process transparent, interactive, and easy to understand, helping users not only find answers, but also understand the reasoning and evidence behind them.Janneta and Jordan also discuss the accelerating pace of AI innovation and the dynamic momentum that startups like Perplexity are driving in the industry, setting new benchmarks in healthcare and research.

David Waldman and Greg Dworkin are here today, with a 50% reduction in staffing projected for tomorrow. You don't even want to know what is planned for Saturday. Hey, any human system will produce simple clerical errors, amirite? And afterwords, lie about it, lie again, and again, then stonewall any investigation, then smear and threaten the investigators, then really threaten the investigators, I mean we've all been there… Oh lord, Kilmar Abrego Garcia is already dead, isn't he? The thing that Trump does, before he quits doing it, is do it as bad as he can, so we can expect a lot worse. People are fighting back even as fighting back becomes riskier. MAGA, meanwhile, demands even more scalps. Trump will get high-flow showerheads and incandescent bulbs and coal power whether anyone else wants them or not. Especially if no one wants them. Who's going to tell him differently? Well, Trump did call off a strike on Iran nuclear sites. We'll probably find out how that happened in the next (Hic) seth leaks… if we don't read it on Signal first. Law firms who had let Donald pussygrab them might soon be dismayed to find him coming back for more. The Ivy League resistance, however, is just getting started. Remember Ron DeSantis? Donald K. Trump doesn't, so Ron has been having an increasingly difficult time keeping accountability from creeping up on him. Speaking of creeps, Frederick Piccolo Jr., a former Ron DeSantis spokesman, was caught exposing his Freddy Piccolo Jr. 4 too many times. Remember Mike Lindell? No, me neither.

We've always thought of ourselves as a little Looney here at Beat of the Month, and this episode we decided to lean into that with an episode all about video games based on cartoons! We cover some old favorites such as Aladdin and The Lion King, some new cartoon based games like Bluey, and then a whole bunch of South Park games - 4 of them to be exact! Throughout the episode we also share a bit of our memories with cartoons and what it was like after a hard day of school and turning on some Tiny Toon Adventures. There's an extra large dose of cartoon game trivia at the end, and we also cover other games that we've been playing lately. Not only that, but we look back all the way to episode #1 for a special clip - lyin' king indeed... So grab your Hi-C ecto cooler, plug in your Walkman, and join us for this episode on Cartoon Games! Oh, and just in case you happen to be new to the show - while the topic might be cartoon games, our show is not for kids!

In this episode of the Epigenetics Podcast, we talked with Vijay Ramani from the Gladstone Institute to talk about his work on Single-Molecule Adenine Methylated Oligonucleosome Sequencing Assay (SAMOSA). Our discussion starts with Vijay Ramani's impactful contributions to the field during his time in Jay Shendure's lab, where he worked on several innovative methods, including RNA proximity ligation. This project was conceived during his graduate studies, aiming to adapt techniques from DNA research to investigate RNA structures—a largely unexplored area at the time. We delved into the nuances of his experiences in graduate school, emphasizing how critical it was to have mentors who provided room for creativity and autonomy in experimental design. Dr. Ramani then shares insights about his foray into developing more refined methodologies, such as in-situ DNA Hi-C, a revolutionary protocol tailored for three-dimensional genomic mapping. He explained the rationale behind his projects, comparing the outcomes with contemporaneous advancements in methods like Micro-C. The discussion highlighted the importance of understanding enzyme bias in chromatin studies and the need for meticulous experimental design to ensure the validity of biological interpretations. We further explored exciting advancements in single-cell genomics, specifically Ramani's work on developing sci-Hi-C. This innovative technique leverages combinatorial indexing to allow high-resolution mapping of chromatin architecture at the single-cell level, a significant leap forward in understanding the complexities of gene regulation. As we progress, Ramani detailed his transition from graduate student to independent investigator starting his own lab. He elaborated on the challenges and excitements associated with establishing his research focus in chromatin structure and function using advanced sequencing technologies. Employing various strategies, including the innovative SAMOSA assay, his research seeks to elucidate the mechanisms by which chromatin structure influences transcriptional regulation. We also discussed the heterogeneity of chromatin and its implications for gene expression. Ramani provided a fascinating perspective on how variations in chromatin structure could affect gene activity, highlighting potential avenues for future research that aims to untangle the complex dynamics at play in both healthy and diseased states.   References Ramani, V., Cusanovich, D., Hause, R. et al. Mapping 3D genome architecture through in situ DNase Hi-C. Nat Protoc 11, 2104–2121 (2016). https://doi.org/10.1038/nprot.2016.126 Nour J Abdulhay, Colin P McNally, Laura J Hsieh, Sivakanthan Kasinathan, Aidan Keith, Laurel S Estes, Mehran Karimzadeh, Jason G Underwood, Hani Goodarzi, Geeta J Narlikar, Vijay Ramani (2020) Massively multiplex single-molecule oligonucleosome footprinting eLife 9:e59404. https://doi.org/10.7554/eLife.59404 Abdulhay, N.J., Hsieh, L.J., McNally, C.P. et al. Nucleosome density shapes kilobase-scale regulation by a mammalian chromatin remodeler. Nat Struct Mol Biol 30, 1571–1581 (2023). https://doi.org/10.1038/s41594-023-01093-6 Nanda, A.S., Wu, K., Irkliyenko, I. et al. Direct transposition of native DNA for sensitive multimodal single-molecule sequencing. Nat Genet 56, 1300–1309 (2024). https://doi.org/10.1038/s41588-024-01748-0   Related Episodes Epigenetic Mechanisms in Genome Regulation and Developmental Programming (James Hackett) Chromatin Profiling: From ChIP to CUT&RUN, CUT&Tag and CUTAC (Steven Henikoff) Split-Pool Recognition of Interactions by Tag Extension (SPRITE) (Mitch Guttman)   Contact Epigenetics Podcast on X Epigenetics Podcast on Instagram Epigenetics Podcast on Mastodon Epigenetics Podcast on Bluesky Epigenetics Podcast on Threads Active Motif on X Active Motif on LinkedIn Email: podcast@activemotif.com

Dawson, returning from spending his whole offscreen summer in Philadelphia with Gale, ends up on a bus next to a very confident pixie-cut blonde. When she disappears after he apparently bores them both to sleep yammering about Joey, he assumes he'll never see her again. He assumes wrong, and is no match for her aggressive come-ons. Her sexual overtures while Dawson's out driving them around in Mitch's boat lead Dawson to make some reckless choices with expensive consequences; knowing this girl works at the local exotic dance establishment, Pacey gets the idea to knock off Risky Business with a stripper party at the Leery house. Through all this, Joey has alternated between parrying the unwanted attention of Rob Logan, her new boss at the marina, and regretting the way she told Dawson off in the Season 2 finale. Roomies Jen and Jack have reached a new level of platonically intimate friendship and are so adorable even Grams seems like they're giving her a cavity -- but in a fun way! Less fun: the tyranny of cheer captain Belinda McGovern, who is terrorizing her classmates with slut-shaming and fatphobia. Jen notices, apparently for the first time, and decides to fight back by trying out for the cheerleading team. But what you really want to know is whether her hair has grown back out: we're thrilled to let you know it has. You made it through the wilderness of our two-week holiday break: welcome back to our episode on "Like A Virgin"! JOIN THE AWT CLUB