Podcasts about junctions

Two days of operator meetings in Melbourne shape the WOMA 2027 agenda, from performance upgrades and cable faults to foundations and bolts. The Uptime Wind Energy Podcast is brought to you by Weather Guard Lightning Tech, creators of the StrikeTape Ultra LPS retrofit. Subscribe to Uptime’s Substack newsletter. And check out Rosemary’s “Engineering with Rosie” Youtube channel. Have a question we can answer on the show? Email us! Allen Hall: Welcome to the Uptime Wind Energy podcast. I’m your host, Allen Hall. I’m here with Matthew Stead and Rosemary Barnes. And Rosemary, where are we?  Rosemary Barnes: We’re in Melbourne, and we have been visiting future attendees and sponsors and people interested in, in the event to see what topics that we should be talking about. What are the hot topics of the moment?  Allen Hall: Which is a very interesting two days, Matthew, in that, uh, we h- did meet with a number of operators based in Australia, but, uh, they’re also very worldly. They have talked to companies all over about operations and, and maintenance, and there are some really eye-opening topics- Mm that will be- Mm … at WOMA 2027 this year.  Matthew Stead: Yeah. The thing that was interesting for me was [00:01:00]that actually the topics have changed each year. So, um, they’re evolving and the industry’s continuing to, to do better, and so that, that was really interesting. Performance upgrades was probably one of the big areas of, of new interest, I think. I think we heard with the pressure on pricing, uh, the market and so forth, you know, that, that 1% or 2% now is becoming more important. Um, so I think, uh, upgrades will definitely feature quite a lot. Um, balance of plant was also a big topic. Um, you just can’t ig- ignore the, um, transmission, you can’t ignore the transformers, you can’t ignore, uh, condensers and all these sorts of things, so yeah. Rosemary Barnes: Cables.  Matthew Stead: Cables.  Rosemary Barnes: Terminations. Terminations of cables. Specifically raised several times.  Allen Hall: Yep. Yeah. It’s all about being more efficient, uh, getting more production, and then with the PPA prices, uh, that are changing-  Matthew Stead: Mm …  Allen Hall: rapidly, uh, everybody is paying more attention to the bottom line.  Matthew Stead: Mm, mm.  Allen Hall: Absolutely. There, there’s less cash running around, uh, chasing new development. It’s [00:02:00] more of a focus on making sure your, at least your existing assets are performing as, as well as they can be- Mm … which then opens up the, uh, Pandora’s box of opportunity. Mm. Because there are 1%’s all around a wind turbine. More specifically, uh, all the drive train issues, the blade issues, the generator issues, and even going into the substation. Mm. I, I was really shocked on BOP e- the, the one topic that came up, uh, yesterday and today was buried cables. Mm. Like-  Matthew Stead: Faults …  Allen Hall: faults.  Rosemary Barnes: Yeah. Junctions. Finding- Yeah … finding faults and what to do about them. Yeah. Yeah. But I think in addition to just wanting more revenue, I think people have, are getting more sophisticated about what actually matters and then the finances. You know, everyone’s focused so much on availability, and now people- Mm … are like, okay, yeah, like once you’re at a certain level of availability, gets harder and harder to get more. And actually, you know, not all availability is equal. Is good. It, it [00:03:00] depends. Yeah. Yeah, like you wanna, um, you wanna focus on how much you’re generating at the times when electricity prices are high specifically, which usually means during lower wind speed periods. Yeah. Which is actually good because that matches really well with what is actually possible. It’s, it’s hard to get more power out of the turbine if it’s at, you know, its rated power, then you’re not g- More efficiency is not gonna get you any more, um, power output, whereas lower in the power curve- Mm … um, when wind speeds are lower, there’s less electricity in the grid and so prices are higher. And so- Mm … yeah, I mean, that’s, that’s why people are really asking to know more about what efficiency upgrades are possible, better ways to operate, scheduled maintenance, um, all those sorts of things. Mm. So that will be really interesting.  Matthew Stead: Raising the bar on sophistication. Um, but also life extension  Allen Hall: Yes, a lot of discussion about life extension  Rosemary Barnes: Yeah, end of life and life extension End of life Yeah, um-  Matthew Stead: Foundations, structures Which is  Allen Hall: tied to PPA. Mm. A lot of that [00:04:00] discussion I, at least I looked at it as, uh, if I had a PPA and I can continue with that PPA with an existing turbine, I want to do that. Mm,  Matthew Stead: mm,  Allen Hall: mm. But how do I do that and how do I know that that existing turbine can last another five years? It seemed to go in five-year blocks, like can we get to another five years and then another and then, then another. Wow.  Rosemary Barnes: Yeah. Once you get to 20, 20, 25 years, I think people like, uh, they, you know- It’s getting a little- Their original agreement might have been for 20, then they kind of just assume that there will be another five, and then after that they’re- Yeah … kind of reassessing cyclically and yeah, I know that people make plans for, you know, what components are replaceable, what would they have to get in, and I think that sometimes, uh, people doing those plans aren’t as familiar with the, you know, actual technical issues that are being faced. Like, is a blade a replaceable component? It may be early on in the life it sort of is, not easily, but you know, like a 25-year-old wind turbine, you know, good luck trying to order 10 new blades because you’ve got a, you know, an issue that’s-  Allen Hall: Oh, well you, you need to read the news. Did you [00:05:00] see the news today about, uh, the, the wooden wind turbine blades as a replacement for aged blades? That, that’s happening- … in real time, Rosa.  Rosemary Barnes: Okay. I know they- Yeah. Yep, yep. Okay. Okay. So that, that’s, that’s good. Making carbon new blade. E- even so, I don’t think that they’re- Okay. … gonna be, like, super-duper cheap, so you’re still gonna wanna be, um, doing a trade-off between y- you know, like, what kind of repairs. You might be able to change the way you’re operating to reduce loads. Um, you might be able to monitor to make sure that your blades are safe. You know, if you know that there’s an issue, um, you just wanna get some advanced warning before blades start falling off your, your tower. Mm. You know, and then you can push it further. Yeah. Whereas if you’ve got no information, then you have to be conservative and shut it down. Yeah. So yeah, I think there’s a whole lot that can be g- can be done there in that area.  Matthew Stead: There was a point that came up today, which I think was close to your heart, on, you know, if you’re going to be going for 1% or 2% or 0.5% AEP improvement, how do you actually measure that and how do you set up a proper experiment? So-  Rosemary Barnes: Yeah …  Matthew Stead: I, I, I love that topic. [00:06:00] Rosemary Barnes: Yeah. No, it’s one that I, um, yeah, I deal with m- my clients I, I’m often… It’s one of the things that Pablo really loves to do, is to organize trials of that nature of new technologies and see if they work. But, um, yeah, asset managers are usually very, uh, focused on fast results. You know, they’ve got a, a big problem and they want a solution now, so they wanna just roll out the new technology over an entire wind farm. But if you do that, then it’s almost impossible. Unless you get, like, a huge benefit, like, uh, 10% gains or, you know, like reduce your failures by 50% or more, it’s really hard to actually- Mm pick that out if you just replace everything. Mm. Whereas if you do a really, really good, um, trial plan- Systematic. Yeah … and you match pairs very well so that you have, you know, a control for each turbine and there’s- so many variables in a wind farm. It’s not, it’s not as easy as just, like, randomly choosing 50% to do and not do. Mm. Mm. Mm. Like, you have to, you know, put some work into the trial design, and then, you know, like, do your statistical calculations ahead of time to know how long it’s gonna take you to get s- statistical [00:07:00]significance. So then you don’t just have a gut feeling about- Yep … how something went. You’ve actually got numbers, and then you can take those numbers to, you know, other wind farms that you’ve got- Yeah and, and know what’s going on much better.  Matthew Stead: Worst study is an inconclusive study.  Rosemary Barnes: Yeah. Yeah, that’s true. Definitely.  Allen Hall: Well, that comes back to the data analysis- Mm … which a lot of operators mentioned, and how much data there is and what to do with it, and it did seem like a couple of operators have chosen some AI tools, varying degrees, all the way down from Microsoft Copilot to something much more complicated. Uh, but I think there’s, uh, getting that d- to the last 1, 2, 3%, you’re going to need those tools- Absolutely … because what do you choose? Do you choose a blade? Do you choose a gearbox? Do you choose a generator? Do you go to the substation to get that percentage point or two Without having some really powerful tools- Mm. you may be wasting your time [00:08:00] and money.  Matthew Stead: Mm.  Allen Hall: Which is a, a, a very interesting, uh, aspect to wind energy because it’s such an industrial business that, uh, we haven’t used heavy computational tools, uh, un- until really now.  Matthew Stead: Mm.  Allen Hall: And maybe Australia’s at the forefront because of the PPA and negative pricing is that that will, uh, actually lead an industry. Because I haven’t seen a lot of that being used globally. Mm. So this is the first time an operator- Mm … that I’ve talked to has said, “We’re using it.”  Matthew Stead: Yeah. It’s quite a different discussion, um, I think, you know, this year compared to previous years in that I think in the past it was getting to know what’s possible, but now it’s like real case studies are coming through. And a few of the people had some really good examples that we can talk about at the conference as to how they’ve actually been helped and how they did this and what the outcome was. I think, yeah, that would be really great content on that.  Rosemary Barnes: Yeah. I think it’s gonna be a good mix of people who have used third party tools and have experience with it, people that are doing it in-house and can share- Mm some of the kinds of results- Mm … that, that you can get just from analyzing your own- Mm … [00:09:00] SCADA data. Um, people talking about how they get the data that they want. Mm. ‘Cause it’s not always so easy. You would think that, you know, you own a wind turbine, you have a right to have all of the data that comes through it, but it’s not. Um, even if you do technically have a right, it’s, uh, it, it’s harder to actually get it than you might think. Mm. So yeah, sharing all, all those kinds of things. But I think also, like just as important as talking about the successes is talking about the, the gaps that– people still feel lots of gaps. Like, okay, we’ve got all the data, we’re collecting it. We know that there’s so much potential here- Mm … but we don’t really- Mm … know what we can do, or it’s hard for us to, you know- Mm … make headway in this particular pain point. And that is really useful for companies that are developing tools to know what are the, the problems. Mm. Because then they can e- you know, they’re well placed to fix them. Allen Hall: Mm. Which leads to the discussion we had with the operators and, uh, some of the suppliers for WOMA 2027. There’s a lot of interest. And as we’re sitting in the conference rooms, I’m thinking, we may not have enough room to [00:10:00] seat everybody. Uh, the WOMA 2027 website is up and running, and you can register now. So just go to woma2027.com and get started there. At the same point, we’ve had a lot of contact with, uh, pretty much everybody that wants to sponsor the event, and there’s only a limited number of ways to sponsor. So if you’re interested in doing that, you, you need to go to woma2027.com and look at those, uh, part- particular packages and see what- Mm fits your, your business. Uh- Going back to some of the, the comments we were just discussing downstairs about what we heard at 2026, like, which is only a couple of months ago, right? It’s back in February this year. Uh, there’s, they’re still discussing what happened at WOMA 2026- Mm. Which was very fascinating- Mm because I think Rosemary, you and I have been to conferences that I have not thought an iota … about what happened at those conferences. Just nothing interesting does occur. There’s no new, new information, there’s [00:11:00] no new science, there’s no new operator approaches.  Rosemary Barnes: Mm.  Allen Hall: But we’re gonna see a number of those- Yeah. Mm … come next March.  Rosemary Barnes: Yeah. Well, I think it’s partly because I don’t know what other conference organizers do, but, you know, we’ve had a exhausting few days here. You’ve come all the way from America, obviously, and, and Claire as well, also come over, our producer. Um, so, y- you know, like, we’re working really hard to make sure that the topics… Like, it’s not an accident that the topics are ones that people are talking about later, because we come here to make sure that we get the right topics. And it’s not just these meetings as well. People get in touch, and- Mm … anybody watching, listening, who has, you know, something that they wanna talk about, then definitely, you know, send us a message, and yeah, we’re working on the agenda. We’ll, we’ll have a draft agenda in the next couple of weeks based on what we’ve learned here, but then we’ve got the hard job of it’s not just that you have a really interesting topic, you need to have a really great speaker- Yeah … or several really great speakers, usually covering several different aspects of the problem. You know, maybe it’s, uh, yeah, an asset [00:12:00] owner, an OEM, and some, some technology provider, you know, all together giving different, um, yeah, perspectives. That’s, I think, what makes a really great session. Mm. So yeah, we need the ideas for the sessions, and we also need the ideas for great speakers.  Matthew Stead: Yeah.  Rosemary Barnes: Right. Matthew Stead: Yeah. I think we’ve, we’ve already matched a few of those dots, so- Yeah … I, we’ve heard people asking for certain topics they wanna hear about, and then we’ve heard other operators saying, “Well, this is what we could talk about.” So I think we’ve already-  Rosemary Barnes: Yeah, yeah … got some great  Matthew Stead: progress.  Rosemary Barnes: It was, it was interesting ’cause we, we built up a list of, you know-  Matthew Stead: Yeah Rosemary Barnes: frequently raised topics, and then you’d say it to the next person that you went to- Mm … and they’re like, “Oh, that’s not a problem for us because we’ve done X, Y, Z.” And you’re like, “Okay. Well, excellent. You can, you can present the solutions that we know that other people- Yeah. Yes … are, are looking for.” Yeah. So it has been… Yeah. Yeah. I mean, it’s definitely worthwhile coming, as, as tiring as it is. Yeah. Um, definitely worthwhile, and we’ll get a much better agenda for the- Yeah … for the effort.  Matthew Stead: And I think, I mean, it has been tiring. Um, but what, what made it for me was one of the operators said that [00:13:00] this is the only conference they will go to So I think, I think we’re doing the right thing. Rosemary Barnes: Yeah. Yeah. I, I think so. I understand too, like, you know, all of us, we kind of are forced to go to events because that’s where our, our clients and customers are, and so you need to see them. And yeah, like I’ve even gone to the extent of some events where I don’t particularly like the event, but I know everyone’s going. Mm. I just go and sit near the event for a couple of days and meet people at a cafe. So yeah, like we can’t get away from it. But if you’re an asset manager or, um, yeah, somebody in that kind of type of work, like you’ve got better things to do than listen to sales pitches aggressively thrown at you that aren’t relevant- Yeah to what you’re doing. So, um, yeah. Like I, I definitely love to hear that kind of feedback- Yeah … and wanna make sure that we get it every, every year. Like that’s- It’s, it’s  Matthew Stead: encouraging.  Rosemary Barnes: Yeah … yeah, that’s, that’s the point of the conference, so.  Matthew Stead: Yeah.  Rosemary Barnes: Yeah.  Matthew Stead: The other big topic was we would really love more OEM involvement. Um-  Rosemary Barnes: Yeah. Everybody wants more- … a lot of the operators- … OEM involvement- Yeah … all of the asset owners.  Matthew Stead: Yeah.  Rosemary Barnes: Like we want to [00:14:00] hear from OEMs more, have them there. Um, so yeah, we’re, we’ll be trying to To get those sorted  Allen Hall: Well, WOMA is a global conference, although it’s Australia-based and there’s a, a number of Australian operators. There are Danish, Americans, uh, plenty of Europeans. Uh, we’re gonna see some from Southeast Asia, I think, this year. Mm-hmm. And, and Japan hopefully will come. Uh, because it’s a, it’s a global conference, there’s global knowledge- Mm … and wind is such a big industry. You may not have the solution in the United States, it may be sitting in Australia, and we need to exchange those ideas. Mm. And that, that’s the point. So w- we are continuing to look for those world experts as we have received all the inputs of these are all the topics we wanna go hear about. Great. Now it’s on us three to go find some of those world experts and, and try to get them to Melbourne. Yeah. And I, I think that’s a great opportunity. So if we do call you and ask you to participate in WOMA [00:15:00] 2027, please take it seriously because- Mm … you will be bombarded with great questions- Mm … and contacts and information. Uh, it’s an event you won’t wanna miss. Yeah. And- But I would- … there’s opportunity there.  Rosemary Barnes: Yeah. I’d say we’re, we’re prioritizing OEMs, um, to, to get more participation, ideally to speak, but at least to be there. And we have heard from multiple asset owners in Australia that they want, they, they want to know what are some of the upgrades that they can do- Yeah … that you’re offering. They wanna know what’s coming next in terms of technology. They wanna know what are some of the non-Western options. So, you know, like it would be great to get some Chinese wind turbine, um, manufacturers as well. Like, they want all this information. They don’t want a slick sales brochure pitch that doesn’t give them any technical information. So we’re hopeful that we’ll be able to get, you know, some technical people to speak on, yeah, what are the upgrades you offer and how does it work- Mm-hmm … and show us a case study that demonstrates the improvements. Um, ’cause that [00:16:00] sometimes is really hard to get out of- Mm … out of OEMs. You know, that, “We’ve got this thing, it’s so amazing, you should get it.” Okay, well, what’s the business case for it? “Oh, well, we don’t have any numbers. Just trust us.”  Matthew Stead: Yeah.  Rosemary Barnes: Um, it’s so common to get a pitch like that. So yeah, any, any OEM that has any- anything like that, either for the next generation of wind turbines or for upgrading the current fleet, yeah, if you’re willing to bring the data, then people, they are desperate to hear this information. Allen Hall: Mm.  Matthew Stead: Yeah. That came up so many times.  Rosemary Barnes: Mm.  Allen Hall: So what were the other, uh, topics that we’re… I’m just not thinking off the top of my head. I know foundations came up quite a bit- Foundations, yeah … which was an odd one, I think, because we haven’t seen that a lot in Australia. Yeah. But this year, foundations, foundations, foundations. Basically, the health of foundations. Yeah.  Matthew Stead: Yeah. I think really there was a lifting of the maturity. Um, I think the topics were sort of showing that, you know, Maslow’s hierarchy and moving into the more of optimization rather than making do, and I think life extension around the foundations was a, a really good example of that. Allen Hall: And bolts.  Matthew Stead: Bolts? Yeah, bolts.  Allen Hall: [00:17:00] Everybody said bolts. Bolts.  Matthew Stead: Bolts.  Allen Hall: You think the world’s simplest device, we’ve been making bolts for nearly 1,000 years or at least a couple hundred. But it does- And, you know- … come up quite often … cable, cable  Matthew Stead: terminations, again, some really  Rosemary Barnes: basic- Yeah, that, really specific ones that were raised multiple times. Yeah. Um, yeah, which, which is great- Yeah … ’cause it gives us a good direction to go. But I do love how it changes so much every year- Yeah … ’cause it makes me feel like, okay, yeah, like we’re actually, you know, it’s worthwhile, um, putting on another event. Mm. Um, not just recording one event and then just, you know, like replaying that every year or something. Re-educating.  Allen Hall: Yeah. Well, I, I think there’s a learning exercise that has happened over the past two years where people now are knowledgeable about those things we talked about- Mm … two years ago. Uh, was it, was it even two years ago? It was a year and a half ago when we first started this, so we’re, we’re not that deep into it. Although our third conference will be next March, uh, you just see more energy, more industry knowledge in some of the references that I heard, uh, in terms of other companies and the approaches they’re taking clearly came from WOMA.  Matthew Stead: Yeah.  Rosemary Barnes: Mm.  Matthew Stead: Yeah,  Allen Hall: yeah. Which is, which is [00:18:00] fascinating. Yeah. That’s  Rosemary Barnes: good. I mean, we want- It, it is  Allen Hall: sticking Rosemary Barnes: we want tech conferences to get better, right? That was the- No … the reason why we, uh, we started this conference was ’cause we didn’t think that it was sufficient, what we had available. So, you know, it’s not a bad thing if other conferences, um, get better. Yeah. Yeah. We should also mention that blades were, were raised a fair bit. We talked so much about blades in the previous years, and we will be talking about blades a lot again, including we’ve got a master class on the Friday. Uh, it’s… Yeah, we’ll go back to some of the basics about how the composite materials work and how a blade is designed and certified and manufactured, and, um- Lightning. Y- yeah, yeah, a little, a little bit about lightning. Um, I don’t wanna cover it too much because we did the master class on lightning- Right … last year. Mm. Um, yeah. And yeah, some of the common damage methods anyway. And of course- Mm … yeah, lightning is probably the most- … common, or I guess leading edge erosion is the most, and then lightning would be the most expensive. Um, yeah, so we’ll be, we’ll be covering all that and just try and raise the knowledge level a little bit, um, for [00:19:00] everybody to… It’s a very complicated kind of, uh, yeah, component.  Matthew Stead: Yeah.  Rosemary Barnes: Yeah.  Matthew Stead: And workshops. So each year we’ve run sort of workshops or roundtables or whatever, so we’re still thinking about the format for them, but, um, thinking about how we’ll reintroduce them again this year. You know, specific topics, specific questions, specific answers.  Rosemary Barnes: Mm.  Allen Hall: And the three of us will be in Hamburg in a couple of weeks at Wind Energy Hamburg, so if you see us and you’re interested in coming to Australia, that’s the place to grab us and- Yep … and shake us and say, “I wanna go to Australia. How do I do it?” Rosemary Barnes: Mm.  Allen Hall: Uh, yeah. Yeah.  Rosemary Barnes: Especially if you’ve got a, a t- technology that addresses some of those specific issues that we’ve mentioned- Mm … then, um, yeah, just know ahead of time that Australia needs, needs more information and more, um, solutions available to them. So, yeah. And  Matthew Stead: that reminds me, we had a really discussion a- around safety and, you know, reasonable and [00:20:00] practicable, and discussion around all the lessons learned about how… or what is best practice on a site, what is best practice about how to manage risks and-  Rosemary Barnes: Mm.  Matthew Stead: Yeah. Yeah. I know you, you, you enjoyed that one.  Rosemary Barnes: Yeah, yeah, definitely. And we’re not talking about, like, safety the- Ear muffs or hats. The, um, rou- Yeah, the routine safety that if you- The goggles, yeah … yeah, trip over and graze your knee, then you need to let the site supervisor know. You, you know, it’s not that stuff, ’cause sites have that under control. That’s a given, yeah. Or at least the ability to get that under control. We’re talking about the bigger- Yeah … bigger things, you know. Like, uh, is there a issue that is causing blades to fall off turbines every now and then? Is there an issue that, uh, can lead to, you know, a fire? I, I think everyone, like- Yeah … a big fear of everybody’s. There’s never been, um, in Australia at least, there’s never been a wind turbine fire that has caused a bush fire, but it is a possibility, and it would be so bad for the industry. So, you know, it’s those things- Mm … that could be just terrible, preventing those before they happen. Mm. So that’s the kind of safety that we’re gonna mainly focus on. Mm. Although, you know, we’re not gonna [00:21:00] turn down questions on, um, some of the smaller stuff as well.  Matthew Stead: Yeah.  Rosemary Barnes: Mm.  Matthew Stead: And we even thought about getting some lawyers-  Rosemary Barnes: Yeah …  Matthew Stead: you missed out on this discussion, Rosie. Okay.  Rosemary Barnes: Yeah.  Matthew Stead: So  Rosemary Barnes: Yeah. Lawyers, this is news to me. Well, I mean, are we talking contracts or, um-  Matthew Stead: No, no … no … it’s really operations. I mean- Right … the environmental, um, issues- Oh yeah … the operational issues. Mm. You know, there’s, there’s a few things in there that the lawyers can add.  Rosemary Barnes: Yeah. I mean- … I’ve, I constantly find myself having to interpret, you know, legal, um, legal text and also anticipate, you know, it’s one thing about what’s the right engineering, but then there is also the legal interpretation of it- Yeah uh, as well, you know, in terms of contract law, but then also in, in terms of safety. Yeah. Um, yeah. Was this certification done correctly? You know- Yeah … that’s got some legal aspect to it. So yeah, that- Yeah … makes sense to me. Insurance is another one where I would say- Yeah, actually that came up … you know, like some, some people might think that that doesn’t sound interesting, but yeah, in- insurance is the other-  Matthew Stead: Mm Rosemary Barnes: aspect that you [00:22:00] just can’t, um, you can’t get away from it. Like, you can’t just think that you’re, you’re doing your engineering without bothering about Mm … yeah, like finance and law and, um, and insurance. Those are things that- Mm … you spend a lot of time thinking about.  Matthew Stead: Yeah. Mm. And I guess we would also love to hear, you know, about potential speakers, but we’d also love to hear any other topics that people are attending and want to know about as well. Rosemary Barnes: Mm-hmm.  Allen Hall: Absolutely. And if you haven’t registered for WOMA 2027, which will be March 3rd through 5th at the Pullman in East Melbourne, right? East Melbourne, yeah. They just redefined it. It’s Pullman in East Melbourne. Uh, go ahead and go to woma2027.com and register now. Uh, Matthew and Rosemary, it’s great to see you in person again, and we’ll see you in a couple of weeks, uh, hopefully in, in Germany. Rosemary Barnes: Mm.  Allen Hall: Very [00:23:00] exciting.

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There has been delay after delay to the junction improvements both crossroads at Durford Road and at The Causeway. Cllr Rob Mocatta said that it is frustrating that has taken so long. He will continue to push for the work to get started as soon as possible, especially at Durford Road that has now seen four accidents since the beginning of this year. Shine Radio's Julie Butler spoke to him about the junctions and other County Council mattersSee omnystudio.com/listener for privacy information.

PJ talks about the junctions that could be improved across the city, hears a harrowing account of a mum walking kilometres to get her daughter to school, celebrates Cork singer Shimmer Boy describing Made In Chelsea using his song. And more... Hosted on Acast. See acast.com/privacy for more information.

Clare County Council has requested an independent safety review of two "notorious" junctions on the M18. It comes amid a heightened focus on the Clare stretch of the motorway following a spate of recent collisions. Much of the focus of late has been closer to the south of the county, with five collisions last month occurring between Cratloe and Dromoland. But two junctions further up have consistently been the scene of near misses and road crashes and have prompted the council to call for an independent review. These are the slip roads and exits respectively at Junction 13 Tulla and Junction 14 Barefield, with multiple motorists raising concerns that the stopping distance is too short for exiting and when attempting to merge. Ennis Councillor Antoinette Baker Bashua says it's particularly dangerous for tourists and day visitors. Another elected representative meanwhile believes average speed camers are needed on the N18 specifically to garauntee safe driver behaviour. Cratloe Councillor Pat O'Gorman says local homeowners feel their taking their lives into their own hands every day.

Die Themen in den Wissensnachrichten +++ DNA in der Stadtluft kann zum Beispiel Drogenkonsum verraten +++ Kam das Fleisch-Räuchern vor dem Kochen und Braten? +++ Bremslichter vorn am Auto für mehr Sicherheit +++**********Weiterführende Quellen zu dieser Folge:Shotgun sequencing of airborne eDNA achieves rapid assessment of whole biomes, population genetics and genomic variation. Nature Ecology & Evolution, 03.06.2025A bioenergetic approach favors the preservation and protection of prey, not cooking, as the drivers of early fire. Frontiers in Nutrition, 16.05.2025Assessment of the Potential of a Front Brake Light to Prevent Crashes and Mitigate the Consequences of Crashes at Junctions. Vehicles, 29.05.2025Measuring historical pollution: Natural history collections as tools for public health and environmental justice research. PNAS, 30.05.2025Observations of the seiche that shook the world. Nature Communications, 03.06.2025Alle Quellen findet ihr hier.**********Ihr könnt uns auch auf diesen Kanälen folgen: TikTok und Instagram .

Adam Hurrey is joined on the Adjudication Panel by Charlie Eccleshare and David Walker. On the agenda: A new world record for away fans slowly realising their team has scored, Mark Schwarzer ties himself in chronological knots, Martin Keown lavishes himself with the highest footballing praise, famously unfriendly footballers' names in American T-junctions and Keys & Gray's ill-fated 2011 bid to break into the app market. Meanwhile, the panel ponder how many consecutive Manchester City defeats it would take for Pep Guardiola to be sacked. Adam's new book, Extra Time Beckons, Penalties Loom: How to Use (and Abuse) The Language of Football, is OUT NOW: https://geni.us/ExtraTimeBeckons Get 10% off the Football Cliches merchandise range for Black Friday at formysins.shop Visit nordvpn.com/cliches to get four extra months on a two-year plan with NordVPN Learn more about your ad choices. Visit podcastchoices.com/adchoices

Live Recorded Set from VirtualDJ Radio TheGrind

Live Recorded Set from VirtualDJ Radio TheGrind

Live Recorded Set from VirtualDJ Radio TheGrind

Live Recorded Set from VirtualDJ Radio TheGrind

The plans of God are greater than the imagination of men. He presets many things in life.

The New Zealand Building Code does not guarantee performance in all areas that many homeowners might expect. Fortunately, Homestar is available to close the performance gaps. Homestar Headline Benefits •    Fresh air •    Dry •    Comfort •    Carbon   Homestar Design Checklist This is a summary of the basic requirements over and above basic building code compliance for achieving 6 Homestar v5. Inclusion of everything here does not guarantee compliance but is a starting point for what should be considered. Homestar Designer: This can be someone within the project team or an external consultant. Engage them in the process as early as possible. Energy model: Homestar v5 uses predicted performance criteria rather than a schedule of allowable insulation levels. The performance criteria include estimated energy consumption, winter and summer internal temperature and likely carbon emissions and these can be calculated using the Energy and Carbon Calculator for Homes (ECCHO). Energy modelling used to be a highly specialised process and expensive process. ECCHO makes energy modelling accessible to Homestar Designers Continuous mechanical ventilation: Internal moisture is a significant problem for New Zealand homes and there is adequate evidence that the building code requirements of openable windows is not reliable for providing enough fresh air and removing dampness. There are simple solutions available for 6 Homestar that are nearly silent, cost very little to run and will provide much more reliable benefits to homeowners. Thermally broken windows: Pay attention to the window frames, not just the glass. Slab edge insulation OR above slab insulation: Junctions such as the connection between walls and floors are risk areas for condensation. Some additional insulation detail can help prevent this and can be easily incorporated when considered early. Water efficient fittings: These are commonly available. Select the best WELS rated products.  For more, visit https://nzgbc.org.nz/introduction-to-homestar

After a whirlwind, whistlestop tour of Thunder Junction and Big Score (featuring Bloomburrow and obliquely Modern Horizons 3 but absolutely NOT Assassin's Creed - yet) we are just about caught up on spoiler season and soon you can be too!Patreon / Twitter / DiscordDom on Patreon

Episode 5: Garrett wants to deliver any good in under ten minutes, for under a quarter, using drones that burrow underground.======(00:00) - Intro(00:50) - What Pipedream is Building(02:17) - Garrett's Background and Learning to Build Businesses(04:02) - How Pipedream Moves Things Efficiently in Cities(07:07) - Why Pneumatic Tubes are Not the Solution(10:08) - How Pipedream's System Works with Robots, Rails, and Totes(13:20) - Designing the Track to Handle Curves and Turns(17:27) - Pipedream's Initial Focus on Instant Pickup and Curbside Delivery(20:12) - The Vision for Edge Warehouses and Hyperlogistics(23:05) - The Key Challenges in Scaling Autonomous Logistics(28:37) - Why Last Mile Delivery Speed is More About Deployment Than Robot Speed(31:10) - The Hardest Problems Pipedream is Solving(37:14) - How Pipedream Decided on Underground Delivery Infrastructure(41:37) - Leveraging Existing Utility Installation and Regulation Practices(44:49) - Believing in the Vision Despite Skepticism(47:53) - The Huge Potential of Last Mile Logistics Innovation(50:51) - Garrett's Journey to Find a Meaningful 10-Year Opportunity(54:49) - Realizing Underground Infrastructure was More Feasible Than Expected(57:22) - Taking the Unconventional Path Despite Discouragement(01:00:00) - The Engineering Appeal and Potential Impact of Last Mile Logistics(01:04:02) - Borrowing from Proven Utility Distribution Models like Water and Sewage(01:07:07) - The Perfect Timing and Conditions for Logistics Disruption(01:11:35) - Cities' Incentives to Support Underground Utility Infrastructure(01:14:27) - Conclusion======Links:Christian Keil – https://twitter.com/pronounced_kyleGarret McCurrach –  https://twitter.com/thegarrettscott Pipedream - https://www.pipedreamlabs.co/======Production and marketing by The Deep View (https://thedeepview.co). For inquiries about sponsoring the podcast, email team@firstprinciples.fm======Checkout the video version here → http://tinyurl.com/4fh497n9

This week I'm interviewing Josh Thompson and discussing all things about his life and healing journey after divorce. Joshua Thompson is the oldest of five siblings and was raised by ‘goodly parents' in the beautiful mountains of Southern California. He served as a full-time missionary in the Washington Tacoma Mission and has served in various callings in The Church of Jesus Christ of Latter-Day Saints. He was married in 2003 in the Bountiful Utah Temple and is the proud father of four children. Unfortunately, his marriage ended in divorce in 2021. Shortly after the divorce, Joshua created the YouTube channel ‘Junctions & Journeys' where he produced approximately 75 videos discussing divorce from the perspective of an active member of The Church of Jesus Christ of Latter-Day Saints. In his videos, he shared some of his personal struggles, triumphs, lessons learned, and various tidbits of inspiration that were part of his healing journey. Most importantly, he shared his testimony of Jesus Christ and the hope that only He can provide. Joshua earned an Associate Degree from BYU-Idaho and a Bachelor's of Science and Master's of Science Degree in Chemical Engineering from the University of Utah. He currently works as an engineering project manager in the oil and gas industry and has called Texas ‘home' since 2011. Joshua is passionate about many things including spending time with his children, eating delicious food, baking, working out, playing pickleball, reading, listening to podcasts, and serving those in need. Thank you so much for listening to this episode! I'm honored and excited to be on this journey to healing and personal growth with you. If you enjoyed the podcast, I'd love to ask you to take 2 minutes to leave me a 5-star review on your podcast app, that way we can help even more men and women find joy in their divorce journies. XO- Jenn

In this episode, Kev and Tracey respond to a listener's request about turning right at junctions.We chat about:The common challenges of turning rightUsing past experiences as a pedestrian Planning and preparationWhile listening to this episode, ask yourself:What aspects of turning right do you find particularly challenging?Have you ever found yourself avoiding specific driving manoeuvres or situations?How do you feel about using past experiences as a pedestrian to judge gaps?Would you like to feature in a future Q&A podcast? Do you have a story that others might relate to? You can record a question about driving nerves or confidence that you would like us to answer on our Speak Pipe page. If you have a question, then there are likely to be others with the same or similar question so ask away and let's start helping more people to build their driving confidence! https://www.speakpipe.com/TheDrivingConfidencePodcastFind out more about how we can help transform the way you feel about driving and follow us on social media:The Drive Calm Journal:A Twelve-Week Prompt Journal for Anxious Drivers - available to buy in all formats on Amazon: https://amzn.eu/d/hdrzRKjThree levels of help on the Confident Drivers website:If you are looking for some free tools and advice, head to our free tools page, where you can sign up for our newsletter and request our Confident Driver coaching wheel.Free tools on our website: https://www.confidentdrivers.co.uk/freetoolsAre you ready to stop the search on social media and start taking action to overcome your driving concerns? Our Nervous Drivers Calming Kit has the online tools and video guides you need to master your driving anxiety. Get immediate access, starting from just £27Nervous Drivers Calming Kit: https://www.confidentdrivers.co.uk/nervous-drivers-calming-kitDo you know that you would like some individual support from us, to create a personalised plan with our help and added accountability to take action? Then our Driving Confidence coaching will help, we offer four different levels of help to suit your needs.One-to-One Driving Confidence Coaching:https://www.confidentdrivers.co.uk/drivingconfidencecoachingLeave us a Tip / Buy us a coffee:https://driving-confidence-podcast.captivate.fm/supportFollow or contact us on Social Media:Facebook: https://www.facebook.com/confidentdriver/Instagram: https://www.instagram.com/confidentdrivers/YouTube: https://www.youtube.com/channel/UCtVucIvyC_2AX33zNL7-yoQPodcast NewsletterIf you would like us to keep you up to date with podcast episodes, click the most appropriate link for...

Welcome to "Behind the Curtain," the captivating theatre podcast that takes you on a thrilling journey beyond the stage. I'm your host, Hollie Nelson, and I'm thrilled to have you joining me on this journey of exploration and discovery. On this weeks episode I'm talking to Reece Kerridge. Reece is a west end performer with a CV including The Book of Mormon, Shrek the Musical, and most recently in the UK Premiere of the SpongeBob Musical playing the role of Old Man Jenkins alongside covering and played SpongeBob and Squidward. Reece also performers at the drag artiste Gracie Lou, who pride herself on a polished poised and professional persona, creating and performing high end cabaret shows with her musical director Michael England. We talk about fitting the jigsaw of a cast, being creative individually, reminding ourselves why we do this and also remembering we are on our own journeys. Whether you're a theatre professional, aspiring actor, or simply someone who loves the magic of the stage, this podcast is your golden ticket. Prepare to be captivated as we pull back the curtain and reveal the secrets, stories, and struggles that bring the stage to life.

Destiny series.

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2023.08.01.551382v1?rss=1 Authors: Choi, H., Liao, Y.-C., Yoon, Y. J., Grimm, J. B., Lavis, L., Singer, R. H., Lippincott-Schwartz, J. Abstract: One-third of the mammalian proteome is comprised of transmembrane and secretory proteins that are synthesized on endoplasmic reticulum (ER). Here, we investigate the spatial distribution and regulation of mRNAs encoding these membrane and secretory proteins (termed 'secretome' mRNAs) through live cell, single molecule tracking to directly monitor the position and translation states of secretome mRNAs on ER and their relationship to other organelles. Notably, translation of secretome mRNAs occurred preferentially near lysosomes on ER marked by the ER junction-associated protein, Lunapark. Knockdown of Lunapark reduced the extent of secretome mRNA translation without affecting translation of other mRNAs. Less secretome mRNA translation also occurred when lysosome function was perturbed by raising lysosomal pH or inhibiting lysosomal proteases. Secretome mRNA translation near lysosomes was enhanced during amino acid deprivation. Addition of the integrated stress response inhibitor, ISRIB, reversed the translation inhibition seen in Lunapark knockdown cells, implying an eIF2 dependency. Altogether, these findings uncover a novel coordination between ER and lysosomes, in which local release of amino acids and other factors from ER-associated lysosomes patterns and regulates translation of mRNAs encoding secretory and membrane proteins. Copy rights belong to original authors. Visit the link for more info Podcast created by Paper Player, LLC

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2023.07.18.549609v1?rss=1 Authors: Choi, I., Jeong, J. Abstract: Tjp1 + is considered a crucial protein involved in the stepwise assembly of tight junctions (TJs) between compaction and blastocoel cavitation in early development. In this study, we investigated the specific role of Tjp1 + in TJ formation by employing an alternative splicing-specific knockdown of the Tjp1 + exon. To deplete Tjp1 + expression, we used siRNA targeting RNA-binding protein 47 (Rbm47), which induces the inclusion of the + exon in Tjp1 mRNA. The knockdown resulted in approximately 85% reduction in Rbm47 mRNA levels and 75% reduction in Tjp1 + mRNA levels in blastocysts. Surprisingly, despite this knockdown, blastocyst development and TJ permeability of trophectoderm were unaffected. Additionally, we observed an interaction between Tjp1 - and Ocln in Rbm47 knockdown blastocysts, suggesting a compensatory role of Tjp1 -. Overall, our findings indicate that Tjp1 + is not essential for the stepwise assembly of TJs and the completion of TJ biogenesis during blastocyst development in mice although a minimal amount of remaining Tjp1 + is sufficient for TJs assembly. Copy rights belong to original authors. Visit the link for more info Podcast created by Paper Player, LLC

An East Clare councillor has expressed fear that two junction on the Limerick to Galway motorway pose a "real danger" to road users. Junction 13 to Ennis, Scarriff and Tulla and Junction 14 to Barefield have come under scrutiny due to the alleged insufficient lengths of their entrance ramps which force motorists to rapidly alter their speed. Councillor Pat Hayes raised the issue at this month's Clare County Council meeting and claimed that many constituents had alerted him to the problem. The Maghera Fianna Fáil councillor says it is unacceptable for modern roads such as these to pose such a threat to the safety of motorists.

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2023.07.10.548446v1?rss=1 Authors: Weesner, J. A., Annunziata, I., van de Vlekkert, D., Robinson, C. G., Campos, Y., Mishra, A., Fremuth, L. E., Gomero, E., Hu, H., d'Azzo, A. Abstract: Endoplasmic reticulum-plasma membrane (ER-PM) junctions mediate Ca2+ flux across neuronal membranes. The properties of these membrane contact sites are defined by their lipid content, but little attention has been given to glycosphingolipids (GSLs). Here, we show that GM1-ganglioside, an abundant GSL in neuronal membranes, is integral to ER-PM junctions; it interacts with synaptic proteins/receptors and regulates Ca2+ signaling. In a model of the neurodegenerative lysosomal storage disease, GM1-gangliosidosis, pathogenic accumulation of GM1 at ER-PM junctions due to {beta} galactosidase deficiency drastically alters neuronal Ca2+ homeostasis. Mechanistically, we show that GM1 interacts with the phosphorylated NMDAR Ca2 channel, thereby increasing Ca2+ flux, activating ERK signaling, and increasing the number of synaptic spines without increasing synaptic connectivity. Thus, GM1 clustering at ER-PM junctions alters synaptic plasticity and exacerbates the generalized neuronal cell death characteristic of GM1-gangliosidosis. Copy rights belong to original authors. Visit the link for more info Podcast created by Paper Player, LLC

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2023.03.06.531372v1?rss=1 Authors: Gurley, N., Szymanski, R., Dowen, R. H., Butcher, T. A., Ishiyama, N., Peifer, M. Abstract: One central question for cell and developmental biologists is defining how epithelial cells can change shape and move during embryonic development without tearing tissues apart. This requires robust yet dynamic connections of cells to one another, via the cell-cell adherens junction, and of junctions to the actin and myosin cytoskeleton, which generates force. The last decade revealed that these connections involve a multivalent network of proteins, rather than a simple linear pathway. We focus on Drosophila Canoe, homolog of mammalian Afadin, as a model for defining the underlying mechanisms. Canoe and Afadin are complex, multidomain proteins that share multiple domains with defined and undefined binding partners. Both also share a long carboxy-terminal intrinsically disordered region (IDR), whose function is less well defined. IDRs are found in many proteins assembled into large multiprotein complexes. We have combined bioinformatic analysis and the use of a series of canoe mutants with early stop codons to explore the evolution and function of the IDR. Our bioinformatic analysis reveals that the IDRs of Canoe and Afadin differ dramatically in sequence and sequence properties. When we looked over shorter evolutionary time scales, we identified multiple conserved motifs. Some of these are predicted by AlphaFold to be alpha-helical, and two correspond to known protein interaction sites for alpha-catenin and F-actin. We next identified the lesions in a series of eighteen canoe mutants, which have early stop codons across the entire protein coding sequence. Analysis of their phenotypes are consistent with the idea that the IDR, including its C-terminal conserved motifs, are important for protein function. These data provide the foundation for further analysis of IDR function. Copy rights belong to original authors. Visit the link for more info Podcast created by Paper Player, LLC

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2023.03.01.530634v1?rss=1 Authors: Schmidt, A., Finegan, T., Haring, M., Kong, D., Fletcher, A. G., Alam, Z., Grosshans, J., Wolf, F., Peifer, M. Abstract: During embryonic development dramatic cell shape changes and movements re-shape the embryonic body plan. These require robust but dynamic linkage between the cell-cell adherens junctions and the force-generating actomyosin cytoskeleton. Our view of this linkage has evolved, and we now realize linkage is mediated by a mechanosensitive multiprotein complex assembled via multivalent connections. Here we combine genetic, cell biological and modeling approaches to define the mechanism of action and functions of an important player, Drosophila Polychaetoid, homolog of mammalian ZO-1. Our data reveal that Pyd reinforces cell junctions under elevated tension, and facilitates cell rearrangements. Pyd is important to maintain junctional contractility and in its absence cell rearrangements stall. We next use structured illumination microscopy to define the molecular architecture of cell-cell junctions during these events. The cadherin-catenin complex and Cno both localize to puncta along the junctional membrane, but are differentially enriched in different puncta. Pyd, in contrast, exhibits a distinct localization to strands that extend out from the region occupied by core junction proteins. We then discuss the implications for the protein network at the junction-cytoskeletal interface, suggesting different proteins localize and function in distinct ways but combine to produce robust connections. Copy rights belong to original authors. Visit the link for more info Podcast created by Paper Player, LLC

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2023.01.31.526419v1?rss=1 Authors: Bragulat-Teixidor, H., Ishihara, K., Szucs, G. M., Otsuka, S. Abstract: The endoplasmic reticulum (ER) is physically connected to the cell nucleus via junctions with the nuclear envelope (NE). These ER-NE junctions are essential for supplying the NE with lipids and transmembrane proteins that are synthesized in the ER. Despite the important role of ER-NE junctions, their biogenesis, architecture and maintenance across the cell cycle has remained elusive. In this study, by combining live cell imaging with quantitative three-dimensional electron microscopy, we systematically elucidated the ultrastructure of ER-NE junctions in mammalian cells. We discovered that ER-NE junctions exhibit a constricted hourglass shape that is different from the junctions within the ER. When ER-NE junctions are newly built during NE assembly at mitotic exit, their morphology resembles ER-ER junctions, but they become constricted starting in telophase. Altogether, our findings imply novel mechanisms that remodel ER-NE junctions and have functional implications for the lipid and protein traffic that are crucial for nuclear function. Copy rights belong to original authors. Visit the link for more info Podcast created by Paper Player, LLC

Frankie interviews:Debbie Peterson is the former Mayor and Councilmember of Grover Beach, California which was the first in her county to recruit and license cannabis businesses. Her business experience equipped her to work on both sides of the dais as an advocate for good government. By the age of 28, Debbie was an award-winning entrepreneur in Great Britain and has negotiated more than five hundred win-win transactions as a residential real estate broker in California. Debbie is the author of the #1 best-seller, The Happiest Corruption: Sleaze, Lies, & Suicide in a California Beach Town and City Council 101 – Insider's Guide for New Councilmembers. https://debbie-peterson.thinkific.com/courses/double-daisMark DePonte is the Owner/Producer of Moosenuts Junction Productions, LLC and the Reality TV Show "Get Up and Go!", that promises a new identity and the life of your dreams. In a world driven by fantastical IG stories and 'I wish I had that life mentality,' this concept is a real head scratcher as we enter 2023. The show will shed some light and test the odds at what could actually happen if you left your life and loved ones behind. The audience would watch as some contestants find that they underappreciated what they once had, while others would finally find success and happiness. Mark's book Get Up and Go! follows the lives of four fictional characters that accept the offer to be on Get Up and Go! https://moosenutsjunction.com/This show is part of the Spreaker Prime Network, if you are interested in advertising on this podcast, contact us at https://www.spreaker.com/show/3240061/advertisement

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2022.11.22.517545v1?rss=1 Authors: Alberici Delsin, L. E., Plutoni, C., Clouvel, A., Keil, S., Marpeaux, L., Elouassouli, L., Khavari, A., Ehrlicher, A., Emery, G. Abstract: Collective cell migration is important for normal development and tissue homeostasis, but can also promote cancer metastasis. To migrate collectively, cells need to coordinate their protrusion formation, rear retraction, adhesion sites dynamics, as well as forces generation and transmission. Nevertheless, the regulatory mechanisms coordinating these processes remain elusive. Using the A431 carcinoma cell line, we identify the kinase MAP4K4 as a central regulator of collective migration. We show that MAP4K4 inactivation blocks the migration of clusters while its overexpression decreases cluster cohesion. MAP4K4 regulates protrusion and retraction dynamics, remodels the actomyosin cytoskeleton, and controls the stability of both cell-cell and cell substrate adhesion. MAP4K4 promotes focal adhesion disassembly through the phosphorylation of Moesin, an actin and plasma membrane cross-linker, but disassembles adherens junctions through a Moesin-independent mechanism. By analyzing traction and intercellular forces, we found that the stabilization of adhesion sites in MAP4K4 loss of function leads to a tensional disequilibrium throughout the cell cluster, increasing the traction forces exerted onto the substrate and the tension loading at the cell-cell adhesions. Together, our results indicates that MAP4K4 activity is a key regulator of biomechanical forces at adhesion sites, promoting collective migration. Copy rights belong to original authors. Visit the link for more info Podcast created by Paper Player, LLC

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2022.11.11.516184v1?rss=1 Authors: Wang, L., Gajwani, P., Chaturvedi, P., Hong, Z., Ye, Z., Schwarz, G., Pohl-Avila, N., Ray, A.-M., Krantz, S., Toth, P. T., Leckband, D. E., Karginov, A. V., Rehman, J. Abstract: Endothelial adherens junctions (AJs) are critical for the regulation of vascular barrier integrity and undergo dis-assembly during inflammatory injury, thus causing vascular leakiness. AJ re-assembly is thus necessary for restoration of the endothelial barrier following the initial injury. Here we examine the metabolic underpinnings that drive restoration of vascular integrity. In response to inflammatory stimuli, the glycolysis regulatory enzyme PFKFB3 is activated, resulting in a rapid and sustained increase of intracellular glycolytic ATP, especially in the proximity of AJs at the plasma membrane. We engineered a novel chemo-genetic construct (RapT) which allowed for precise temporal control of PFKFB3 recruitment to the plasma membrane. Activation of RapT by rapamycin during the barrier restoration phase increased regional ATP and accelerated AJ re-assembly. Mechanistically, we observed that PFKFB3 is activated through two modes. Src-mediated post-translational phosphorylation rapidly increases PFKFB3 activity. Using another chemo-genetic approach to temporally control Src activity, we found that Src activates PFKFB3 by binding to and phosphorylating it at residues Y175, Y334, and Y363. Tyrosine-phospho-deficient mutants of PFKFB3 at these residues block the glycolytic activation upon inflammatory stimuli. In parallel, elevated reactive oxygen species generated during inflammatory stimulation create pockets of regional hypoxia and allow for increased Hif1-mediated transcription of PFKFB3, leading to sustained glycolytic activation. Moreover, inhibition of PFKFB3 delays AJ reassembly and restoration of vascular integrity both in vitro and in vivo. In conclusion, we show that while inflammatory activation acutely compromises the endothelial barrier, inflammatory signaling also concomitantly generates a metabolic milieu in anticipation of the subsequent re-assembly of AJs and restoration of the vascular barrier. Copy rights belong to original authors. Visit the link for more info Podcast created by Paper Player, LLC

This weeks episode Ryan and Robin chat about the importance of balance when it comes to consuming beer, Ryan's recent big changes away from Social Media and recent studies on the health impacts of over consumption.   They also talk about Oktoberfest, what fall and winter beer styles they are excited to get their hands on in the next couple months and highlight a few upcoming Anniversaries including Laylow's 6th and Junctions 10th!   Thanks for tuning in!

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2022.09.13.507821v1?rss=1 Authors: Doney, E., Dion-Albert, L., Coulombe-Rozon, F., Osbourne, N., Bernatchez, R., Paton, S. E. J., Kaufmann, F. N., Agomma, R. O., Solano, J. L., Gaumond, R., Dudek, K. A., Szyszkowicz, J. K., Signature Consortium,, Lebel, M., Doyen, A., Durand, A., Lavoie-Cardinal, F., Audet, M.-C., Menard, C. Abstract: Major depressive disorder (MDD) is the leading cause of disability worldwide. However, 30-50% of patients are unresponsive to commonly prescribed antidepressants, highlighting untapped causal biological mechanisms. Dysfunction in the microbiota-gut-brain axis, the bidirectional communications between the central nervous system and gastrointestinal tract that are modulated by gut microorganisms, has been implicated in MDD pathogenesis. Exposure to chronic stress disrupts blood-brain barrier integrity, still, little is known about intestinal barrier function in these conditions particularly for the small intestine where most food and drug absorption takes place. Thus, here we investigate how chronic social or variable stress, two mouse models of depression, impact the jejunum (JEJ) intestinal barrier in males and females. Mice were subjected to stress paradigms followed by analysis of gene expression profiles of intestinal barrier-related targets, fecal microbial composition, and blood-based markers. Altered microbial populations as well as changes in gene expression of JEJ tight junctions were observed depending on the type and duration of stress, with sex-specific effects. We took advantage of machine learning to characterize in detail morphological tight junction properties identifying a cluster of ruffled junctions in stressed animals. Junctional ruffling is associated with inflammation, so we evaluated if LPS injection recapitulates stress-induced changes in the JEJ and observed profound sex differences. Finally, LPS-binding protein (LBP), a marker of gut barrier leakiness, was associated with stress vulnerability in mice and translational value was confirmed on blood samples from women with MDD. Our results provide evidence that chronic stress disrupts intestinal barrier homeostasis in conjunction with the manifestation of depressive-like behaviors in a sex-specific manner in mice and possibly, human depression. Copy rights belong to original authors. Visit the link for more info Podcast created by PaperPlayer

Thank you Valeria, Chelsea and Natalia for the excellent questions! Keep them coming! Today we answer some of your very interesting geoscience questions ranging from how the moon formed to what's going on in Vinicunca Peru! We talk about the Mendocino Triple Junction, one of the most rapidly rising places on Earth, the magma ocean's that formed the moon, and how sedimentary rocks can get such spectacularly beautiful coloring to them! Like, Subscribe, and leave us a Rating! ——————————————————Instagram: @planetgeocastTwitter: @planetgeocastFacebook: @planetgeocastEmail: planetgeocast@gmail.comWebsite: https://planetgeocast.com/

Lots of behind the scenes from a Yachtley Crew show during Midnight At The Oasis car show in Yuma Arizona, a whole lot of epiphanies, a new Fun Fact from Henry D. Horse and more! Plus Maria Humpreys from the Podcast Support Community on Facebook, comedian Craig Spivek, Micky Dolenz from The Monkees and singer/songwriter Blythe Baines all say hello! --- Send in a voice message: https://anchor.fm/inspiradoprojecto/message Support this podcast: https://anchor.fm/inspiradoprojecto/support

Listen as Paul Abernathy, CEO and Founder of Electrical Code Academy, Inc. answers another question that was submitted via PaulAbernathy.com and the Ask Paul Portal. This submission asks about the differences in 312.2 and 314.15 when dealing with wet and/or damp locations.

Listen as Paul Abernathy, CEO and Founder of Electrical Code Academy, Inc. answers another question that was submitted via PaulAbernathy.com and the Ask Paul Portal. This submission asks about the differences in 312.2 and 314.15 when dealing with wet and/or damp locations.

So how serious is this new variant of COVID, Long Time Oregon Congressman Peter Defazio wont run again and Cave Junction Oregon...home of the Tarzan Swing. Click Here for video

In this episode, we review the high-yield topic of Epithelial Cell Junctions from the Dermatology section. Follow Medbullets on social media: Facebook: www.facebook.com/medbullets Instagram: www.instagram.com/medbulletsofficial Twitter: www.twitter.com/medbullets --- Send in a voice message: https://anchor.fm/medbulletsstep1/message

Listen as Paul Abernathy talks about Section 300.14 and Free Conductors length necessary in junction boxes, outlet boxes, switch points within boxes, and so on.--- This episode is sponsored by · Anchor: The easiest way to make a podcast. https://anchor.fm/app--- Send in a voice message: https://anchor.fm/electricianlive/messageSupport this podcast: https://anchor.fm/electricianlive/support

Overgrown grass verges and hedges across the North West region are turning road junctions into death traps, according to local residents, who are urging local authorities to deal with the matter. Donegal Cllr Niamh Kennedy and Sligo Cllr Thomas Healy both have had representations on the matter

Need help with the theory test? Sign up to the 5 Minute Theory training course here: https://tcdrive-learning.teachable.com/ (https://tcdrive-learning.teachable.com/) where you'll get access to over 40 training videos to help you learn, understand and pass your theory test! We continue our look into the top 10 faults of the UK driving test, today looking at 'incorrect positioning when turning right at junctions.' You can find the full DVSA list https://www.gov.uk/government/publications/top-10-reasons-for-failing-the-driving-test (here). Feedback, suggestions or questions? Find us at https://www.tcdrive.co.uk/theory.html#/ (TCDrive.co.uk) or follow us on social https://twitter.com/TCDrive1 (Twitter), https://www.facebook.com/TCDrivingSchool (Facebook), https://www.linkedin.com/in/terry-cook-5233b719a/ (LinkedIn) and https://www.instagram.com/tcdriving_school/ (Instagram).

This episode is also available as a blog post: http://biopatrika.com/2021/05/13/interview-mechanical-instability-cell-junctions-regulates-fate-hair-follicle-stem-cells/

Need help with the theory test? Sign up to the 5 Minute Theory training course here: https://tcdrive-learning.teachable.com/ (https://tcdrive-learning.teachable.com/) where you'll get access to over 40 training videos to help you learn, understand and pass your theory test! In this episode we look at some key theory questions around (yellow) box junctions. We discuss if and under what circumstances you're allowed to stop in them.  If you'd like to gain more practice on the theory test, head over to https://www.tcdrive.co.uk/#/ (TCDrive.co.uk) where you can find lots of useful information and get access to our theory test training course. You can also follow us on social media on - https://www.facebook.com/TCDrivingSchool (Facebook), https://www.instagram.com/tcdriving_school/ (Instagram), https://www.linkedin.com/in/terry-cook-5233b719a/ (LinkedIn), https://twitter.com/tcdrive1 (Twitter )and https://www.tiktok.com/@that_driving_guy?lang=en (TikTok).

Listen as Paul Abernathy talks about Section 300.14 and Free Conductors length necessary in junction boxes, outlet boxes, switch points within boxes and so on.

Gravity Duals of Interfaces, Defects and Junctions in N=4 SYM

Joe chats to Geraldine Herbert motoring correspondent with the Sunday Independent about the plans for the new average speed safety camera system See acast.com/privacy for privacy and opt-out information.

This episode covers epithelial cell junctions!

耳朵學英文 大腦篇#6，突觸 synapses synopsis (n.) a short description of the contents of something such as a film or book （電影、書籍等的）概要，梗概，提要 ..... BBC, NY Times, Match (詞組搭配) 每月開班！ 請私訊林威老師 lineID: linwayet 各位同學好，我是林威老師， 英文教學已達27年 講解BBC 720篇文章(3年)， 經濟學人2100篇文章 (8年) 花了三年的時間整理的終極片語， 豐富的例句中英對照 本書前面有53個重要的字根， 以及字首字尾整理 本書本的最後還整理了 兩個動詞make和take的慣用語的比較 只要購買字根200回影片講解 (雲端分享)，贈送本書, 歡迎點選demo影片 ! 
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我有個商品要賣『林威老師親編終極片語+影片講解200個字根』，售價$6,000！快到我的店鋪看看吧！https://shopee.tw/product/18811006/6072162816?smtt=0.18812342-1609723528.4 #蝦皮購物 ...... The human brain weighs approximately 1.4 kg and is made up of billions of cells called neurons. Junctions between neurons, known as synapses, enable electrical and chemical messages to be transmitted from one neuron to the next in the brain, a process that underlies basic sensory functions and that is critical to learning, memory and thought formation, and other cognitive activities. 人腦重約1.4公斤，由數十億個稱為神經元的細胞組成。神經元之間的連接稱為突觸，使電和化學信息可以從一個神經元傳遞到大腦中的另一個神經元，這一過程是基本的感覺功能的基礎，對學習，記憶和思維形成以及其他認知活動至關重要。 .... In the nervous system, a synapse is a structure that permits a neuron (or nerve cell) to pass an electrical or chemical signal to another neuron or to the target effector cell. Synapses are essential to the transmission of nervous impulses from one neuron to another. 在神經系統中，突觸是允許神經元（或神經細胞）將電或化學信號傳遞到另一個神經元的結構。突觸對於神經衝動從一個神經元到另一個神經元的傳遞至關重要。 ...... In the central nervous system, a synapse is a small gap at the end of a neuron that allows a signal to pass from one neuron to the next. Synapses are found where nerve cells connect with other nerve cells. Synapses are key to the brain's function, especially when it comes to memory. 突觸是大腦功能的關鍵，特別是在記憶方面。 The term synapse was first introduced in 1897 by physiologist Michael Foster in his "Textbook of Physiology" and is derived from the Greek synapsis, meaning "conjunction." ... Neurons have specialized projections called dendrites and axons. Dendrites bring information to the cell body and axons take information away from the cell body. 神經元本身除了有 cell body，還有樹突和軸突。樹突將信息帶到細胞體，軸突將信息帶離細胞體。

การลดน้ำหนักให้ได้ 10 กิโลกรัมน่าจะเป็นสิ่งที่หลายคนปรารถนา แต่ก็อาจไม่เหมาะกับทุกคนเสมอไป New Year New You: First Time ชวน โค้ชฟ้าใส-ฟ้าใส พึ่งอุดม จาก Fit Junctions มาคุยเรื่องวิธีการลดน้ำหนักที่ถูกต้อง ทำอย่างไรถึงจะสามารถลดน้ำหนักได้สำเร็จ วิธีการทานอาหารที่ถูกต้องต้อง พร้อมเทคนิคการปรับพฤติกรรมง่ายๆ ที่ใครก็ทำได้ตั้งแต่การเริ่มต้นลดน้ำหนักเป็นครั้งแรก

Aprenda como usar couldn't, wouldn't shouldn't e outros neste episódio. --- Support this podcast: https://anchor.fm/agenciadiferent/support

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2020.10.22.351148v1?rss=1 Authors: Leckband, D., Rehman, J., Schwarz, G., Gajwani, P. Abstract: One of the central functions of the endothelium is to maintain a vascular barrier that prevents fluid leakiness and immune cell influx from the circulating blood into the tissue. The barrier integrity of the endothelium is largely controlled by adherens junctions (AJs) and the key AJ molecule VE-cadherin, which maintains cell cohesion via homotypic trans-interaction with VE-cadherin molecules on neighboring endothelial cells. Tension is required to maintain junction homeostasis, but little is known about the role of endothelial metabolism and bioenergetics in regulating junctional forces. Because glycolysis is the main source of ATP generation in endothelial cells, we examined the bioenergetic control of the mechanics of VE-cadherin junctions, by focusing on the glycolysis regulatory enzyme 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3 (PFKFB3). Results from traction force imbalance measurements and a VE-cadherin tension sensor revealed that inhibiting PFKFB3 significantly reduced the average junctional tension and the force on VE-cadherin complexes. The decrease in tension was largely due to mechanical changes distal from the cell-cell contacts. Specifically, inhibiting glycolysis perturbs focal adhesions and disrupts actin organization, directly impacting the net force on intercellular contacts. These findings identify a critical role of cellular metabolism for the mechanics and integrity of vascular endothelial junctions, by maintaining global cell mechanics. Copy rights belong to original authors. Visit the link for more info

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2020.09.24.293886v1?rss=1 Authors: Mejia Maza, A., Jarvis, S., Lee, W. C., Cunningham, T. J., Schiavo, G., Secrier, M., Fratta, P., Sleigh, J. N., Sudre, C. H., Fisher, E. M. C. Abstract: The neuromuscular junction (NMJ) is the peripheral synapse formed between a motor neuron axon terminal and a muscle fibre. NMJs are thought to be the primary site of peripheral pathology in many neuromuscular diseases, but innervation/denervation status is often assessed qualitatively with poor systematic criteria across studies, and separately from 3D morphological structure. Here, we describe the development of NMJ-Analyser, to comprehensively screen the morphology of NMJs and their corresponding innervation status automatically. NMJ-Analyser generates 29 biologically relevant features to quantitatively define healthy and aberrant neuromuscular synapses and applies machine learning to diagnose NMJ degeneration. We validated this framework in longitudinal analyses of wildtype mice, as well as in four different neuromuscular disease models: three for amyotrophic lateral sclerosis (ALS) and one for peripheral neuropathy. We showed that structural changes at the NMJ initially occur in the nerve terminal of mutant TDP43 and FUS ALS models. Using a machine learning algorithm, healthy and aberrant neuromuscular synapses are identified with 95% accuracy, with 88% sensitivity and 97% specificity. Our results validate NMJ-Analyser as a robust platform for systematic and structural screening of NMJs, and pave the way for transferrable, and cross-comparison and high-throughput studies in neuromuscular diseases. Copy rights belong to original authors. Visit the link for more info

In this episode we discuss the importance of evaluating and addressing the spinal junctions (C0-C1, Cervicothoracic Junction and Thoracolumbar Junction). From a clinical perspective, this is something we see is missed a lot when we get clients who rehab failed them before. We discuss what we see/hear/feel clinically which tunes us in to look into the junctions and treat it appropriately! Clinical Chat starts at 2:58 Sips Provided By: Flying Fish Brewing

Listen as Paul Abernathy settles the long-standing debate of pre-twisting wire connectors, such as Wire Nuts, a trademark of ideal, and Gorilla Nuts, a trademark of King Innovation. Check out this short podcast where it is finally answered.....Kinda...LOL

Listen as Paul Abernathy settles the long-standing debate of pre-twisting wire connectors, such as Wire Nuts, a trademark of ideal, and Gorilla Nuts, a trademark of King Innovation. Check out this short podcast where it is finally answered.....Kinda...LOL

How is West Seattle showing and embracing LGBTQ Pride? We found out in stories from all three Junctions, plus an update from the Farmers Market.

Watch video podcast here on Urban Cycling Institute's YouTube channel. To kick off the podcast, we will be discussing in order the eight chapters in the CROW Design Manual for Bicycle Traffic: 1. The development of bicycle traffic 1.1 The importance of the bicycle 1.2 The history of the bicycle 1.3 The bicycle and government policy 1.4 The role of the bicycle 2. Cycle-friendly design 2.1 Policy as foundation 2.2 Cycle-friendly infrastructure 2.3 The cyclist as a measure for the design 2.4 Main requirements cycle-friendly infrastructure 2.5 Integrated designing 3. Basic data 3.1 Bicycle dimensions 3.2 (Design) speed, accelerating and braking 3.3 Stability, deviation and clearance 3.4 Bends and view 3.5 Inclines 3.6 Patterns in bicycle use 3.7 Fellow users of cycle facilities 4. Design of the cycle network 4.1 The basis of any design 4.2 Levels in quality 4.3 Requirements for the main cycle network 4.4 Establishing main cycle network 4.5 Bicycle highways 4.6 Recreational cycle network 5. Road sections 5.1 Function, design and use 5.2 Requirements for a road section 5.3 Solitary cycle paths and cycle/moped paths 5.4 Bicycle traffic and motorized traffic within built-up areas 5.5 Bicycle traffic and motorized traffic outside of built-up areas 5.6 Special situations 6. Junctions 6.1 Function, design and use 6.2 Requirements for a junction 6.3 Junctions according to road type 7. Implementation, maintenance and furnishings 7.1 Road surfacing 7.2 Verges and plants 7.3 Lighting 7.4 Signage 7.5 Personal safety 7.6 Other facilities 8. Evaluation and management 8.1 Inspecting and evaluating bicycle connections 8.2 Inspecting surfacing for cyclists 8.3 Measures in the case of work in progress 8.4 Winter maintenance for the benefit of cyclists Order the CROW Design Manual for Bicycle Traffic https://crowplatform.com/product/design-manual-for-bicycle-traffic/ Connect with Urban Cycling Institute: Website - https://urbancyclinginstitute.com Twitter - https://twitter.com/fietsprofessor Facebook - https://www.facebook.com/urbancyclinginstitute Linkedin - https://www.linkedin.com/company/urbancyclinginstitute Coursera - https://www.coursera.org/learn/unraveling-the-cycling-city

Hello and welcome to our JRPG hyper fixation cast! We talk about Final Fantasy a lot. Just like. A lot. Music in this episode is a selection from this album of lo fi FF7 beats and the Blue Dragon final boss theme

Hello and welcome to our JRPG hyper fixation cast! We talk about Final Fantasy a lot. Just like. A lot. Music in this episode is a selection from this album of lo fi FF7 beats and the Blue Dragon final boss theme

Cloverdale Bibleway Sunday Evening January 26, 2020

In the latest dispatch from the Fortress of Proopitude, Greg and Jennifer reflect on Robert De Niro, Reading and The Rutles.

Quick episode today. Arrividerci, Turdwater.

Live electronics (real-time recording).

86. Give Thyself Wholly

A new system of queuing lorries if there are delays at Dover has been tested out on the M20. Operation Brock was put into place at 6am and is replacing the old Operation Stack. HGVs heading to the port have been using the coastbound stretch of the M20 between Junctions 8 and 9 - a contraflow's been in place for all other traffic on the London bound carriageway, with two lanes in each direction. https://www.kentonline.co.uk/

Show recorded on 16/10/18 40 minutes In which we take a look at ideas for innovative ideas for modern junctions and road markings. Modern Junctions Sat Nav Usage 1 Year Old Test Reference Points Road Haulage Stats Links Holographic Crossing .

Why It Matters Episode 1 Duration: 9:10 mins Synopsis: In this new podcast series for 2018, The Straits Times takes a close look at one key news talking point each week. In the wake of two fatal accidents in April 2018, the Land Transport Authority has decided - where feasible - to replace discretionary right turns with red-amber-green arrow lights at the bulk of road junctions in Singapore.  Host and ST's deputy news editor Jeremy Au Yong dissects the issue with ST transport correspondent Adrian Lim. Produced by: Jeremy Au Yong & Ernest Luis Feedback to: podcast@sph.com.sg Download ST & BT podcasts for offline listening: http://str.sg/stbtpodcasts On website: http://str.sg/stpodcasts On Spotify: https://spoti.fi/2PwZCYU On iTunes: https://apple.co/2Lu4rPP On Google podcasts: http://str.sg/googlestbt

Why It Matters Episode 1 Duration: 9:10 mins Synopsis: In this new podcast series for 2018, The Straits Times takes a close look at one key news talking point each week. In the wake of two fatal accidents in April 2018, the Land Transport Authority has decided - where feasible - to replace discretionary right turns with red-amber-green arrow lights at the bulk of road junctions in Singapore.  Host and ST's deputy news editor Jeremy Au Yong dissects the issue with ST transport correspondent Adrian Lim. Produced by: Jeremy Au Yong & Ernest Luis Feedback to: podcast@sph.com.sg Download ST & BT podcasts for offline listening: http://str.sg/stbtpodcasts On website: http://str.sg/stpodcasts On Spotify: https://spoti.fi/2PwZCYU On iTunes: https://apple.co/2Lu4rPP On Google podcasts: http://str.sg/googlestbt See omnystudio.com/listener for privacy information.

In this episode of Ask Anatomist, we explore snake venom with Dr. Wayne Hodgson.

Weekend #BikeAdventure

Eine effektive Regulation der Gewebedurchblutung erfordert eine Koordination der Reaktion einzelner Gefäßzellen bzw. verschiedener Gefäßabschnitte. Der zur Koordination erforderliche interzelluläre Signalaustausch kann zumindest teilweise über Gap Junction-Kanäle erfolgen, die als interzelluläre Verbindungen den Austausch von elektrischen und chemischen Signalstoffen zwischen benachbarten Zellen ermöglichen. Dieser Austausch kann über die Modulation der Permeabilität von Gap Junction-Kanälen reguliert werden. Aus Untersuchungen an Modellzellen (HeLA-Zellen) war bereits bekannt dass NO eine solche Modulatorwirkung ausübt, wenn die Gap Junctions nur Connexin 37 (Cx37) enthalten während kein Effekt von NO auf Gap Junctions zu beobachtet war, wenn Gap Junctions aus Cx43 oder Cx40 gebildet wurden. Da Endothelzellen normalerweise alle drei Connexine exprimieren, sollte in der vorliegenden Arbeit untersucht werden, inwieweit NO in diesen Zellen überhaupt eine nachweisbare Wirkung auf die Gap Junction Permeabilität und damit auf den Signalaustausch entfaltet. Als Modell des Signalaustauschs wurde die Ausbreitung von Calciumwellen jeweils zwischen Endothelzellen oder glatten Muskelzellen allein oder zwischen beiden Zelltypen untersucht. Nach Auslösung von interzellulären Calciumwellen als Folge einer mechanischen Stimulation von einzelnen Zellen konnte zunächst gezeigt werden, dass die interzelluläre Ausbreitung von Calcium unter den gewählten Versuchsbedingungen über Gap Junctions-erfolgte. Im Gegensatz zum Modellsystem der HeLa Zellen, in denen nur Cx37 exprimiert war, zeigte NO in den Endothelzellen (humane Nabelschnur, alle drei Connexine exprimiert) abgesehen von einer geringradigen Verzögerung keinen Hemmeffekt auf die Gap Junction-abhängige Ausbreitung von Calcium-Signalen. Wurde jedoch Cx43 durch Behandlung mit siRNA herunterreguliert, führte NO auch in den Endothelzellen zu einer Hemmung der interzellulären Calciumwellenausbreitung. Auch in intakten Endothelzellen, die mit glatten Muskelzellen kokultiviert wurden, ließ sich bei genauerer Analyse ein Hemmeffekt von NO nachweisen. Dieser war jedoch auf die Zellbereiche beschränkt, in denen Endothelzellen und glatte Muskelzellen unmittelbar benachbart waren (myoendotheliale Junctions). In diesen myoendo-thelialen Gap Junctions, fanden wir auf der Endothelseite immunhistochemisch überwiegend Cx37 exprimiert. Aufgrund dieser präferentiellen Lokalisation von Cx37 scheint daher NO eine besondere Rolle bei der Modulation des Calciumaustauschs (und potentiell auch anderer Signalmoleküle wie IP3 oder cyclische Nukleotide) zu spielen. Die Kontrolle des Calciumaustauschs könnte funktionell eine calciumabhängige glattmuskuläre Kontraktion bei Endothelstimulation verhindern und somit die endothelabhängige Dilatation verstärken. Diese bisher unbekannte NO-Wirkung auf Cx37-exprimierende Gap Junctions könnte einen weiteren Mechanismus der Gefäßtonusregulation darstellen.

Lectures aimed at giving an introduction to the definitions and meanings of simple block diagrams and easy to understand methods for computing relationships between loop inputs and outputs. Lecture 4 focuses on the role and interpretation of a summing junction. Thanks to viewer who noticed a typo at 8.44 - an obvious missing G in final step!

Fri, 22 Nov 2013 01:23:37 GMT https://sakai.rutgers.edu/access/content/group/c7b3269a-bc92-488e-b4b1-0e20648073af/Podcasts/Lectur

Welcome to UKADI, the only website you need with all the information, advice, tips and videos to help you pass the driving test at your next attempt.As a driving instructor with over 10 years experience helping thousands of learner drivers get the highly coveted DSA pass certificate, I know what it takes to please the DSA examiners, and my goal is through this online instructional blog to give you free the resources needed to reach the standard expected of every test candidate.A word of caution, passing the UK driving test is not easy, and you as a learner driver will increase your chances of passing first time by putting in the hard work and effort (this does not mean just taking driving lessons only), as many very good drivers with competent car handling skills have failed the test due to simple things that can only be blamed on either a lack of knowledge of the UK driving examiner requirements or over-confidence not giving the DSA practical test the due credit it deserves as one of the toughest learner assessments in the world.The UK Driving TestTo get a full UK driving licence that enables you to drive a car in the UK unsupervised (manual or automatic transmission), you need to pass 2 tests (1 computer based theory and a practical vehicle motorcycle, car, HGV/LGV assessment).If you have not already done so, you should apply for your provisional driving licence, and while you are waiting for it to arrive, I suggest you get copy of the official DSA learner guide shown below and start preparing for your theory test right away (it is never too early to start preparing). The guide also contains information about the practical driving test as well, and since it is made by the people who actually conduct the assessment, it is all you need!Most people pass the theory test first time especially if they take the time to go through the official dsa study guides like the one mentioned above, but if you don't just study some more, practise the hazard perception videos and you should be fine. Note that the test is available to take in other languages, see the DSA website for an up to date list.Once you have taken and passed the theory test, you have 2 years to take and pass the practical assessment or your theory test pass certificate will expire and you will have to go through the process again, including paying another booking fee.Preparing and taking the Practical Driving TestI have already mentioned that passing the UK driving test is not easy, to illustrate this, of the 1.7m tests conducted in the 2011/2012 financial year only 47% passed, that means over 900,000 people failed! (source DSA Press Release), so it is important that you are fully prepared (practically and knowledge wise) if you do not want to be one of those failure statistics.How to Prepare for the UK practical driving test?PRIVATE TUITION (Learning with Friends/Family)PROFESSIONAL Driving Lessons with DSA approved InstructorPrivate TuitionCurrently in the UK there are no requirements that stops people from taking the driving test even if they have not used a DSA qualified instructor to learn, nor do you have to bring a dual controlled instructor's vehicle to the test centre, so this is a common option used by many, but having spoken to a few examiners in my local centres a high percentage of these candidates fail for a few reasons.1. Lack of knowledge: Friends/family rarely have all the complete knowledge to adequately prepare a provisional licenced learner driver for the dsa driving test. To supervise a learner you need to have at least held a full licence for 3 years. Within that time, changes could have been made which an ordinary driver might not know about. Another thing to consider is that most drivers would have forgotten some of the things that they were taught by their instructor that made them pass, so they can't pass skills that they do not have or remember.2. Bad Habits: Friends/Family teaching you to drive will always pass on or not notice some unsafe bad driving habits that you have which will in turn increase your chances of not passing the driving test.3. Inadequate Preparation: Most candidates learning to drive with friends/family will think they are ready for the practical car test once they have learnt the 4 dsa driving maneuvers, can drive from A-B and feel confident. DSA driving tests are designed to assess a candidate's ability not only to drive a car from A-B, but they will take you on different road types, traffic conditions, junctions, meeting situation and in all this the examiner wants to see you being SAFE.Driving test routes can be tricky and sometimes having local knowledge might be beneficial especially when your teacher knows exactly how the DSA examiner expects things to be done. Have you practised driving on a 70mph dual carriageway including moving into the right hand lane to turn right at traffic lights or a roundabout?The DSA recommends 40 hrs of professional lessons with additional private practice to gain enough experience and confidence to pass the driving test first time.So what advice can I give for those who would like to learn to drive with family or friends due to high cost of driving lessons.1. Know what is required. It is very easy to pass a test when you know what you will be tested on, as you can prepare adequately by focusing on the core skills. For those learning to drive with friends I recommend you get a copy of the Official DSA Guide to Driving. This book tells you how the DSA want you to drive or perfom a maneuver, and is a required reference to know that what you are being taught is wrong or right.Many learner drivers will trust their dads when it comes to driving, but they can be wrong, and you can use this book to stay on track or request to do things differently as at the end of the day the DSA examiner is using these standards, so if you want to pass do things the DSA way. A common reason a lot of learners fail is not knowing the highway code, so this book covers all situations and rules that pertain to driving in the UK.2. Take a few professional lessons or mock test. Only a qualified approved driving instructor can truly tell if you have reached the standard required by an examiner, so taking a mock test with them will reveal how ready you are, what bad habits you exibit and you would have a chance of driving on a typical dsa test route.The best way to find a good driving instructor is to go by recommendation from a friend who has used one, failing that you can use the DSA website to find an instructor.When speaking to an instructor, take note if they answer the phone while on a driving lesson (giving directions/instructions), as this is already a bad sign that they are not professional.More reading for learners using private tuition to learn to driveTop 10 Driving Test FaultsAre You Ready for The Driving Test (video)Expensive Driving Lesson with Dad!Professional Driving LessonsThe most common question a driving instructor gets asked is how many lessons will I need to be ready for a driving test? There is no simple answer to that question, there are a lot of factors that determine this, including choosing the right instructor. However here is some advice to help you get the best out of your lessons and increase the chances of you passing first time.1. Get a copy of the learners record. Even though your instructor will be guiding you in your preparations, the learners driver record will serve as tracker on your progress against the skills being measure by the DSA.2. Make good use of your time between driving lessons. If you are really serious about passing the driving test first time, then you need to make use of your free time to continue to learn about what the DSA examiners expect during the test. You can watch videos about the manuevers you are having problems with, memorise reference points, and most importantly of all know the highway code inside out!Any one who has taken driving lessons with me or read this free online blog would know that I am constantly recommending the highway code for test candidates for one simple reason, you need to fully know the rules of the road, including signs and road markings.Yes as a driving instructor I will tell you anything you need to know, but knowing the highway code inside out is not only beneficial to you on the driving test, but once you pass, you are less likely to be banned for having 6 points on licence after passing test or get a parking ticket for parking in the wrong place.As I mentioned at the beginning of the article, those who put in extra work are more likely to pass at the first attempt compared to those who barely take enough lessons, hardly know enough signs, only been on a high speed dual carriageway twice and are hoping they do not get the reverse round the corner exercise as they are rubbish at it.Why Do People Fail The UK Driving Test?Not everyone who fails the driving test is totally unprepared, sometimes test nerves can play a part, while others arrive too late, but for those who do take the test, here are the top 5 reasons and the serious driver errors marked by the DSA examiner.JunctionsThe most common place learner drivers commit a serious major driving fault is at junctions where the fault is usually that of ineffective observations.Many driving test candidates approach junctions too fast, leaving themselves not enough time to use the MSPSL Routine. If you can't see that it is 100% safe to go at the junction, you SHOULD wait. The examiner does not expect you to be as fast as experienced drivers, neither can you take some of the risks that they do on the test.ManeuversWhenever carrying out any of the 4 reversing maneuvers, there are 2 skills that the examiner expects from you, effective observations to keep you safe and good car control to enable you carry out the exercise itself.You should not hit the kerb, be too wide when reversing round a corner, straddle 2 bays at the end of the bay parking exercise and must parallel park within 2 car lenghts.Mirror ChecksFailing to check mirrors before changing speed (slowing down or accelerating), changing direction or not acting on the information seen in the mirrors. Do not get into the habit of just moving your head when signalling, you MUST process the image in the mirrors and act on what you see. Remember you driving must not affect or potentially impact other road users.Moving Off SafelyWhile stalling the car is not usually a serious driving error, if you try to pull off in 3rd gear, and stall your vehicle half-way into a junction where you are an obstruction to others, then you have no chance of the examiner not marking a major serious fault.The other usual reason candidates fail moving off is not checking the right blind spot after stopping on the left handside of the road or causing another car to slow down/change direction as you move into the road.Use of Signals/IndicatorsThe common errors here, are not signalling when necessary, poor timing of signals, misleading signals and finally forgetting to cancel a signal after coming off a junction.More Resources and Tips to Help Pass The UK Driving TestDealing with Mini Roundabouts.Points on Licence and Driving Test.Simple Driving Test Mistakes That Fail LearnersDSA Examiner Driving Test RoutesAs an instructor over the years I have seen some very good drivers get tripped up at some tricky places on some of the routes by examiners in the NW London area, so I have made these tutorial videos to cover these situations.Each video covers what I have found out to the toughest route, and has additional tips on other places or things that you should be aware as a driving test candidate. These are offered as complimentary preparation materials to your lessons or private practice sessions.Hendon Driving Test Routes Video 2012£4.99 Buy NowIndependent Driving On the Test.Theory Test ChangesDriving Test Changes.Mill Hill Driving Test Route Video£4.99 Buy NowBorehamwood Test Centre WD6 Route Videos£4.99 Buy NowForiegn Licence UK Driving TestOver the 10 years of being a driving instructor, I have helped many drivers from countries like America, Canada, Pakistan, Nigeria, Soth Africa, etc pass the driving test first time, something they would not have been able to do on their own for ONE KEY reason BAD DRIVING HABITS.If you are required to take a test instead of exchanging your foriegn licence for a UK one, the authorities (DSA/DVLA) do not believe your country's driving standards are good enough to use here! For this very reason you SHOULD take a few lessons from a qualified instructor to weed out the bad habits, as well as know what the examiners expect. Many candidates with international driving permits who pass the theory test first attempt go on to take a practical assessment thinking that the DSA examiner just wants to see if they can confidently drive from A to B.It is more than just that, which is why they are surprised when the bad news is delivered at the end of the 40 minute assessment.A 1hr driving lesson can not get rid of driving habits! I have seen so many drivers try this thinking they are smart by asking for one session on the dsa examiner routes, and while I will give them ALL the help I can, habits don't die that easily. Usually they start off fine, but 20 minutes in, they relax (especially once they start talking with the examiner) and they the normal habits show up (driving with one hand, no mirror checks, harsh braking, excessive speed).If you are serious about passing, you should consider taking a 2 hr session at least a week before the test so you know what is expected, and can then practise (if you have an insured car with international permit) or take a few more lessons to iron the bad driving out.Thinking about becoming an instructor? Don't just make a decision based on the red driving school TV advertisement, do the proper research and know all the ADI training options available to you.Subscribe to my Driving Test Tips so you don't miss any future articles and get DSA updates direct to your inbox by Email. The service is provided and powered by Google Feedburner, so I don't personally keep your email addresses, and you can remove yourself anytime after passing the driving test with just one mouse click, you can also follow UKADI on twitter or join my UKADI Facebook page.Please let me know your views by posting a comment on the blog at http://www.ukadi.co.uk

A situation which is not common but you might still come across on your driving test is zebra crossings just before junctions especially roundabouts and this instructor video contains some important advice on how to deal with these situations according to the DSA standard.To have the best chance and avoiding failing the driving test, I would advice you to subscribe by email to get free driving lesson videos sent to your inbox. Add Driving School videos to your Feed Reader

Many learner drivers fail the UK driving test at Junctions. This Driving School Video gives advice on how to prepare for junctions and deal with them the way the DSA examiner expects.Make sure you are fully prepared for the practical car driving test, just because you rarely stall on driving lessons and know the examiner routes doesn't mean you are at the required standard.Subscribe by email to get free driving lesson videos sent to your inbox. Add Driving School videos to your Feed Reader

juncTions was inspired by the Toronto-based keyboard collective junctQín. In 2009, junctQín asked me to write a piece for three pianists performing on one piano. Their request triggered several interesting questions for me: What can be achieved on a piano if one person plays the instrument in a traditional way (pressing the keys and pedals) while a second one is playing the inside (with an e-bow and a tone bar, tools more commonly used by guitar players) and a third musician is operating a computer program that creates an immersive maelstrom of sounds by processing the sonorities of the two piano players? The inside and outside sound worlds of the piano confront and connect with each other. For example, connections are intentionally achieved when one player creates a glissando by sliding a piece of metal along the very strings which are excited, hit by the hammers the second player has activated. At first glance the score may look pretty sparse. However, those delicate sound processes that are created by the two players at the piano are fed into a computer program which densely superimposes them and projects them into the space. In this piece, the piano is used as a versatile sound device with a flexible resonator. All the harmonic relationships of the notes are based on four odd-numbered overtone series (starting with the low A key) which are collapsing into the middle B of the piano. This tone acts like a secret harmonic thread, often emphasized by an E-Bow which can infinitely sustain sound, contradicting the expected decay of a piano note. In juncTions, a type of hyper-instrument is created from a common piano, freed from its inherent historical connotations; no longer referencing the piano music between Mozart and Stockhausen ... The only thing that matters now is the expansion of its sound into the space. Info: http://www.essl.at/works/junctions.html

The crista junction is a tubular element of the mitochondrial inner membrane connecting inner boundary membrane and cristae membrane. The molecular basis of its formation was so far largely unknown. The lack of the mitochondrial inner membrane protein mitofilin results in the absence of crista junctions in HeLa-cells. Moreover, deletion of Fcj1, the orthologue of mitofilin in S. cerevisiae, leads to concentric stacks of cristae membranes and the virtual absence of crista junctions. The aim of the present work was to investigate the function of Fcj1 as well as the molecular basis of the formation of cristae and crista junctions in S. cerevisiae. Fcj1 undergoes homotypic interactions and is part of a stable oligomeric protein complex. Using immuno-EM, Fcj1 has been shown to be specifically enriched at crista junctions. All other proteins, which have been studied so far, such as subunits Su e and Su g of the F1FO-ATP-Synthase, are rather underrepresented in this region. Overexpression of Fcj1 increases the number of crista junctions, enlarges the crista junction diameter and leads to internal branching of cristae. Upon downregulation of Fcj1, the mitochondrial ultrastructure progressively changes reflecting the deletion phenotype more and more. Considering the fact that the decrease of the number of crista junctions occurs at an early time point, Fcj1 seems directly involved in the formation of crista junctions. In order to investigate if cristae form a detached compartment in the absence of crista junctions, the accessibility of the intracristal space for the protein-modifying substance AMS was examined. In fcj1 mitochondria the modification of cytochrome c was temporally delayed and incomplete in comparison to wild-type. Hence, metabolite exchange between intracristal space and cristae space seems impaired though not entirely impossible in the absence of crista junctions. This might be due to some extent of fusion and fission tracing back to protein or lipid exchange within the inner membrane of mitochondria. Cryo-EM tomograms revealed regular zipper-like arrangements of F1FO-ATP-Synthase particles. An inverse correlation of the amount of Fcj1 and the oligomeric state of F1FO-ATP-Synthase was observed upon biochemical analysis: in fcj1 mitochondria, F1FO-ATP-Synthase oligomers have a higher molecular weight and are more stable than in wild-type mitochondria, whereas in mitochondria of cells overexpressing Fcj1 they are smaller and less stable. Subunits Su e and Su g are essential for the stabilization of dimers and oligomers of the F1FO-ATP-Synthase. As the influence of Su e/Su g on the formation of crista junctions has not been investigated so far, the mitochondrial ultrastructure of both deletion strains was analyzed concerning this matter. Enlargement of crista junctions and internal branching of cristae similar to the overexpression phenotype of Fcj1 have been observed in both strains. Furthermore, crista tips are virtually absent. Apart from this functional interaction, Fcj1 and Su e/Su g have also been shown to interact genetically. Based on the gained insights on the roles of Fcj1 and Su e/Su g in cristae formation the following model is proposed: the modulation of the inner membrane of mitochondria is based on an antagonistic behavior of Fcj1 and Su e/Su g. Fcj1 introduces negative membrane curvatures, possibly by impairing the oligomerisation of the F1FO-ATP-Synthase. The presence of Su e/Su g, in contrast, promotes positive curvatures of cristae membranes by stabilizing dimers and oligomers of the F1FO-ATP-Synthase. Taken together, relative amounts of Fcj1 and Su e/Su g locally modulate the oligomeric state of the F1FO-ATP-Synthase and thereby enable the formation of crista junctions and crista tips in mitochondria.

King Solva explains the inherent dangers of traveling through Junctions and informs Alex and his group that they may never return home. Jenneva learns a shocking truth that explains the origin of the Universes.

Jenneva and Egam visit the dwarves while Aurora and Dalgar discover the nature of Universes and Junctions.

We talk more about holes today. They don't really exist, you know! But when only a few electrons are missing from the valence band, it's so much more convenient to describe only the missing states that the fictional particles we call "holes" are a very useful concept. We talk more about their mass, velocity, momentum, and other properties. Then we discuss the p-n junction, where a semiconductor surface is donor-doped on, say, the right, and acceptor-doped on, say, the left. We calculate the strength of the permanent electric field that happens at the interface. This permanent electric field produces a real live voltage in the material. Can you use it to run a light bulb?Due to technical difficulties this year, I post last year's lecture:Lecture Audio