Podcasts about gtex

Aliya Grig, a visionary entrepreneur, is leading groundbreaking initiatives in quantum computing, AI, and biomedical innovation. Her work spans developing quantum algorithms, building quantum hardware, creating AI-powered manufacturing solutions, and researching longevity. Grig's unique approach to AI, inspired by human consciousness, aims to create more efficient and creative problem-solving systems. Her ambitious Cosmos City project in UAE aims to create a hub for space tech and future tech startups, fostering collaboration across disciplines. 00:09- About Aliya Grig Aliya is the founder of the Evolwe and Kosmos Foundation. She is a researcher in AGI, developing a cutting-edge AGI architecture that integrates principles from consciousness studies, neuroscience, and cognitive science to create a more advanced and adaptable system. She is a Karman Space Foundation member and a mentor at a Growth Mentor platform. She is also speaking and judging global innovation at GTEx, which is the world's largest tech conference. --- Support this podcast: https://podcasters.spotify.com/pod/show/tbcy/support

BUFFALO, NY- April 15, 2024 – A new #researchpaper was #published on the #cover of Aging (listed by MEDLINE/PubMed as "Aging (Albany NY)" and "Aging-US" by Web of Science) Volume 16, Issue 7, entitled, “Evidence of a pan-tissue decline in stemness during human aging.” Despite their biological importance, the role of stem cells in human aging remains to be elucidated. In a new study, researchers Gabriel Arantes dos Santos, Gustavo Daniel Vega Magdaleno and João Pedro de Magalhães from the Universidade de Sao Paulo, University of Birmingham and the University of Liverpool applied a machine learning method to detect stemness signatures from transcriptome data of healthy human tissues. “In this work, we applied a machine learning methodology to GTEx transcriptome data and assigned stemness scores to 17,382 healthy samples from 30 human tissues aged between 20 and 79 years.” The team found that ~60% of the studied tissues exhibit a significant negative correlation between the subject's age and stemness score. The only significant exception was the uterus, where they observed an increased stemness with age. Moreover, the researchers observed that stemness is positively correlated with cell proliferation and negatively correlated with cellular senescence. Finally, they also observed a trend that hematopoietic stem cells derived from older individuals might have higher stemness scores. “In conclusion, we assigned stemness scores to human samples and show evidence of a pan-tissue loss of stemness during human aging, which adds weight to the idea that stem cell deterioration may contribute to human aging.” DOI - https://doi.org/10.18632/aging.205717 Corresponding author - João Pedro de Magalhães - jp@senescence.info Sign up for free Altmetric alerts about this article - https://aging.altmetric.com/details/email_updates?id=10.18632%2Faging.205717 Subscribe for free publication alerts from Aging - https://www.aging-us.com/subscribe-to-toc-alerts Keywords - aging, longevity, stem cells, transcriptomics, senescence About Aging-US Aging publishes research papers in all fields of aging research including but not limited, aging from yeast to mammals, cellular senescence, age-related diseases such as cancer and Alzheimer's diseases and their prevention and treatment, anti-aging strategies and drug development and especially the role of signal transduction pathways such as mTOR in aging and potential approaches to modulate these signaling pathways to extend lifespan. The journal aims to promote treatment of age-related diseases by slowing down aging, validation of anti-aging drugs by treating age-related diseases, prevention of cancer by inhibiting aging. Cancer and COVID-19 are age-related diseases. Aging is indexed by PubMed/Medline (abbreviated as “Aging (Albany NY)”), PubMed Central, Web of Science: Science Citation Index Expanded (abbreviated as “Aging‐US” and listed in the Cell Biology and Geriatrics & Gerontology categories), Scopus (abbreviated as “Aging” and listed in the Cell Biology and Aging categories), Biological Abstracts, BIOSIS Previews, EMBASE, META (Chan Zuckerberg Initiative) (2018-2022), and Dimensions (Digital Science). Please visit our website at https://www.Aging-US.com​​ and connect with us: Facebook - https://www.facebook.com/AgingUS/ X - https://twitter.com/AgingJrnl Instagram - https://www.instagram.com/agingjrnl/ YouTube - https://www.youtube.com/@AgingJournal LinkedIn - https://www.linkedin.com/company/aging/ Pinterest - https://www.pinterest.com/AgingUS/ Spotify - https://open.spotify.com/show/1X4HQQgegjReaf6Mozn6Mc Media Contact 18009220957 MEDIA@IMPACTJOURNALS.COM

Welcome to another episode of Expert To Authority Show, brought to you by http://gtex.org.uk/,  I am your host, Simone Vincenzi, The Experts Strategist, and this is the podcast for experts who want to become the ultimate authority in their niche while making an impact in the world. We have created the Webinar Conversion Kit where you will get access to: The High-Converting Webinar Framework BONUS #1: High-Converting Webinar Slide Template BONUS #2: Pitch and Follow Up Templates BONUS #3: High Converting Webinars Case Studies BONUS #4: Our Trello Webinar Checklist All of this for only £29.99 for a limited period of time. Click here to download. https://webinarconversionkit.com/ Today I have the pleasure to interview Deborah Maw  Entertaining and informative, Dr Deborah Maw, is passionate about integrating the teachings of science, creativity, Astrology and Tarot to help each individual tap into their unique potential and wonder. With a bubbly and hopeful personality, Deborah brings a sense of fun and positivity to her presentations which range from the Science of Astrology, to Unlocking Our Creative Genius and the Art of Play. Inspired by the GTex partnership training Deborah has just completed a 3 month, 17 cities tour with ‘Wild, Wyrd, Wise, the Astrology of 2024' for which she won a coveted GTex Speaker of the Year Award. A van dweller, frequently seen drumming in a Manchester park, Deborah began her career as a researcher in Biophysics before ‘dropping out' at age 25. She found herself on a lifetime Spiritual Journey, which included studying Astrological Psychology, and achieving her teenage desire to go to art college for four years. A true Digital Nomad she travels all over the world speaking and inspiring. Deborah began training with GTex in 2023 and since then, working entirely from her phone Deborah posts a daily astrology and tarot tip on just about every social media platform, facilitating a spiritual training day by day. She has gathered a significant following. Connect with Deborah Maw   Facebook Link: https://www.facebook.com/profile.php?id=100087460425971 Instagram Link: https://www.instagram.com/deborah_creativegenius LinkedIn Link: https://www.linkedin.com/in/dr-deborah-maw YouTube Link: https://youtube.com/@DrDeborahMaw-SpiritualMa-mk7tv To become a GTeX Member, Apply here: https://gtex.events/call  ------- To receive daily support in your coaching and speaking business, join our private Facebook Group EXPLODE YOUR EXPERT BIZ https://www.facebook.com/groups/explodeyourexpertbiz/ ------- Take a full business assessment for free to have absolute clarity on your business with the EXPERT BIZ CHECKLIST. http://bit.ly/expert-biz-checklist-podcast ------ Also, make sure you subscribe to the podcast so you don't miss any other episode.   If you want to reach out to me with your questions, you can email me at Simone@gtex.org.uk that comes right to my inbox. --- Send in a voice message: https://podcasters.spotify.com/pod/show/explode/message

Get to know these successful thought leaders and find out how they present themselves and their crafts as experts in their fields. Brian Bogert is a human behavior and performance coach, keynote speaker, business strategist, author, and community leader. He helps growth-minded individuals achieve the best version of themselves– their true authentic selves. As a result of his innovative and dynamic leadership, Brian Bogert was acclaimed as one of the “40 Under 40” by the Phoenix Business Journal. If you are an entrepreneur and deep down, you don't know who you really are, it is best to reach out to Brian Bogert by logging on to www.brianbogert.com or by visiting his LinkedIn page at https://www.linkedin.com/in/brianbogert/.  Lucy Hoger is a successful corporate board member, CEO, and senior operating executive. She has continuously provided vision and propelled profitable business growth to take organizations and companies to the next level of achievement within competitive markets. She has proven herself repeatedly as a leader for spearheading the turnaround of potential failures into successes, reengineering operations for optimal results, and innovating technology solutions and business strategies that open the doors to new and expanded markets with long-term viability and growth prospects, even in volatile economies. If you recognize that you have a leaky bucket and that you need to figure out not just how to fill or empty it, you should reach out to Lucy Hoger by visiting her website at https://lucyhoger.com/ or https://www.linkedin.com/in/lucyhoger/. Simone Vincenzi is a multi-award-winning serial entrepreneur and author who helped people launch more than 500 profitable businesses before he reached the age of 30 with his company GTeX. He is passionate about building thriving community-led businesses where the members are the core and essence of the decisions and development of ideas. If you're an expert and are tired of monthly launches, you have to reach out to Simone Vincenzi by visiting his website at https://gtex.org.uk/.  Global Credibility Expert, Mitchell Levy is a TEDx speaker and international bestselling author of over 60 books. As The AHA Guy at AHAthat (https://ahathat.com), he helps to extract the genius from your head in a two-three hour interview so that his team can ghostwrite your book, publish it, distribute it, and make you an Amazon bestselling author in four months or less. He is an accomplished Entrepreneur who has created twenty businesses in Silicon Valley including four publishing companies that have published over 800 books. He's provided strategic consulting to over one hundred companies, and has been chairman of the board of a NASDAQ-listed company. Mitchell has been happily married for thirty years and regularly spends four weeks in Europe with family and friends. Visit https://mitchelllevy.com/mitchelllevypresents/ for an archive of all the podcast episodes.   Connect to Mitchell Levy on: Credibility Nation YouTube Channel: https://www.youtube.com/c/CredibilityNation  Credibility Nation LinkedIn: https://www.linkedin.com/company/credibilitynation/ Mitchell Levy Present AHA Moments: https://mitchelllevy.com/mitchelllevypresents/ Thought Leader Life: https://thoughtleaderlife.com Twitter: @Credtabulous Instagram: @credibilitynation Learn more about your ad choices. Visit megaphone.fm/adchoices

Welcome to another episode of Expert To Authority Show, brought to you by http://gtex.org.uk/,  I am your host, Simone Vincenzi, The Experts Strategist, and this is the podcast for experts who want to become the ultimate authority in their niche while making an impact in the world. We have created the Webinar Conversion Kit where you will get access to: The High-Converting Webinar Framework BONUS #1: High-Converting Webinar Slide Template BONUS #2: Pitch and Follow Up Templates BONUS #3: High Converting Webinars Case Studies BONUS #4: Our Trello Webinar Checklist All of this for only £29.99 for a limited period of time. Click here to download. https://webinarconversionkit.com/ Today I have the pleasure to Interview Claudia Romero  Tired of generic advice? Claudia brings innovation to the table, crafting tailored strategies for unique needs. As a mother, coach, and speaker, she understands the juggling act of business and family. With her S.O.U.L System, say farewell to overwhelm and welcome efficiency. Claudia's journey, from a lazy student to a powerhouse entrepreneur, is proof that anything is possible. Despite challenges like relocating to Scotland and battling cancer, she remains dedicated to helping others succeed. Claudia is on a mission to bring calm to the busy business world, guiding individuals to pursue passions without stress or regrets. Together, let's expedite your journey to success. If Claudia can do it, so can you! Let's make productivity fun, fulfilling, and truly life-changing! Connect with Claudia Romero   Facebook Link: https://www.facebook.com/ClaudiaRomeroCoaching/ Website:  https://claudia-romero.com/ LinkedIn Link: https://www.linkedin.com/in/claudiaromerocoaching/ To become a GTeX Member, Apply here: https://gtex.events/call  ------- To receive daily support in your coaching and speaking business, join our private Facebook Group EXPLODE YOUR EXPERT BIZ https://www.facebook.com/groups/explodeyourexpertbiz/ ------- Take a full business assessment for free to have absolute clarity on your business with the EXPERT BIZ CHECKLIST. http://bit.ly/expert-biz-checklist-podcast ------ Also, make sure you subscribe to the podcast so you don't miss any other episode.   If you want to reach out to me with your questions, you can email me at Simone@gtex.org.uk that comes right to my inbox. --- Send in a voice message: https://podcasters.spotify.com/pod/show/explode/message

Welcome to another episode of Expert To Authority Show, brought to you by http://gtex.org.uk/,  I am your host, Simone Vincenzi, The Experts Strategist, and this is the podcast for experts who want to become the ultimate authority in their niche while making an impact in the world. We have created the Webinar Conversion Kit where you will get access to: The High-Converting Webinar Framework BONUS #1: High-Converting Webinar Slide Template BONUS #2: Pitch and Follow Up Templates BONUS #3: High Converting Webinars Case Studies BONUS #4: Our Trello Webinar Checklist All of this for only £29.99 for a limited period of time. Click here to download. https://webinarconversionkit.com/ Today I have the pleasure to Interview Angela Roth  Angela Roth began her coaching career as a weight loss specialist, helping clients escape from the trap of yoyo dieting, but when Covid hit, life took an unexpected change of direction! Moving from face to face meetings, into the virtual world didn't go quite as planned, and Angela found herself spending more time supporting other coaches with building their brand and online presence, than with helping people lose weight.  She also discovered that she enjoyed her new role immensely! In January 2022, she decided it was time to shift and launched her Business Community for Coaches, Succeed From The Start, and she hasn't looked back ever since.  However, in 2023, Angela's Mum was taken seriously ill and she knew that there was another priority in her life, one that she was certainly not going to shy away from. Her Mum needed her, it was as simple as that. So life changed again, and Angela began a different chapter of sharing her work, and her home life with her Mum, over 300 miles away. Living a split life wasn't  completely new for Angela, a similar need had arisen before Covid when one of her son's had needed support in recovery from domestic abuse, so with her husband's full support, Angela took on the challenge again, determined to make things work both for her family and her business. Receiving the Grit award from GTex gave her a much needed boost of encouragement, knowing that there were others who were there to support her along the way. Connect with Angela Roth  Website Link: www.succeedfromthestart.com LinkedIn Link: https://www.linkedin.com/in/angela-roth-succeed-from-the-start/ https://www.facebook.com/angela.roth.coach/ To become a GTeX Member, Apply here: https://gtex.events/call  ------- To receive daily support in your coaching and speaking business, join our private Facebook Group EXPLODE YOUR EXPERT BIZ https://www.facebook.com/groups/explodeyourexpertbiz/ ------- Take a full business assessment for free to have absolute clarity on your business with the EXPERT BIZ CHECKLIST. http://bit.ly/expert-biz-checklist-podcast ------ Also, make sure you subscribe to the podcast so you don't miss any other episode.   If you want to reach out to me with your questions, you can email me at Simone@gtex.org.uk that comes right to my inbox. --- Send in a voice message: https://podcasters.spotify.com/pod/show/explode/message

Welcome to another episode of Expert To Authority Show, brought to you by http://gtex.org.uk/,  I am your host, Simone Vincenzi, The Experts Strategist, and this is the podcast for experts who want to become the ultimate authority in their niche while making an impact in the world. We have created the Webinar Conversion Kit where you will get access to: The High-Converting Webinar Framework BONUS #1: High-Converting Webinar Slide Template BONUS #2: Pitch and Follow Up Templates BONUS #3: High Converting Webinars Case Studies BONUS #4: Our Trello Webinar Checklist All of this for only £29.99 for a limited period of time. Click here to download. https://webinarconversionkit.com/ Today I have the pleasure to Interview Carol Anne Cooper  Carol Anne assists entrepreneurs to shine, by getting their story written. She is creator and leader of the M.O.V.E. Method writing programme ‘Let's Write', developed through her own journey from Maths teacher to becoming The Writing Facilitator, discovering tools and techniques that have assisted her in unlocking the writer within. Connect with Carol Anne Cooper   Facebook Link: https://www.facebook.com/carolanne.cooper.96 Instagram Link: https://www.instagram.com/carol.anne.writing.facilitator LinkedIn Link: https://www.linkedin.com/in/carolannecooper/ To become a GTeX Member, Apply here: https://gtex.events/call  ------- To receive daily support in your coaching and speaking business, join our private Facebook Group EXPLODE YOUR EXPERT BIZ https://www.facebook.com/groups/explodeyourexpertbiz/ ------- Take a full business assessment for free to have absolute clarity on your business with the EXPERT BIZ CHECKLIST. http://bit.ly/expert-biz-checklist-podcast ------ Also, make sure you subscribe to the podcast so you don't miss any other episode.   If you want to reach out to me with your questions, you can email me at Simone@gtex.org.uk that comes right to my inbox. --- Send in a voice message: https://podcasters.spotify.com/pod/show/explode/message

Welcome to another episode of Expert To Authority Show, brought to you by http://gtex.org.uk/,  I am your host, Simone Vincenzi, The Experts Strategist, and this is the podcast for experts who want to become the ultimate authority in their niche while making an impact in the world. We have created the Webinar Conversion Kit where you will get access to: The High-Converting Webinar Framework BONUS #1: High-Converting Webinar Slide Template BONUS #2: Pitch and Follow Up Templates BONUS #3: High Converting Webinars Case Studies BONUS #4: Our Trello Webinar Checklist All of this for only £29.99 for a limited period of time. Click here to download. https://webinarconversionkit.com/ Today I have the pleasure to Interview Sarah Poynton Ryan  Sarah Poynton Ryan have been a property investor since 2011 and she believe in living a life of choice rooted in financial independence. She released her book Money Mechanics in 2023 and she is now on a mission to help people to engineer their money using the low risk debt and investing tactics of the rich. Connect with Sarah Poynton Ryan   Website:  https://www.cogito-wealth.co.uk/ Facebook Link: https://www.facebook.com/cogitowealth  Instagram Link: https://www.instagram.com/cogitowealth  LinkedIn Link: https://www.linkedin.com/company/cogitowealth Book: www.sarahpoynton.com/moneymechanics  To become a GTeX Member, Apply here: https://gtex.events/call  ------- To receive daily support in your coaching and speaking business, join our private Facebook Group EXPLODE YOUR EXPERT BIZ https://www.facebook.com/groups/explodeyourexpertbiz/ ------- Take a full business assessment for free to have absolute clarity on your business with the EXPERT BIZ CHECKLIST. http://bit.ly/expert-biz-checklist-podcast ------ Also, make sure you subscribe to the podcast so you don't miss any other episode.   If you want to reach out to me with your questions, you can email me at Simone@gtex.org.uk that comes right to my inbox. --- Send in a voice message: https://podcasters.spotify.com/pod/show/explode/message

Welcome to another episode of Expert To Authority Show, brought to you by http://gtex.org.uk/,  I am your host, Simone Vincenzi, The Experts Strategist, and this is the podcast for experts who want to become the ultimate authority in their niche while making an impact in the world. We have created the Webinar Conversion Kit where you will get access to: The High-Converting Webinar Framework BONUS #1: High-Converting Webinar Slide Template BONUS #2: Pitch and Follow Up Templates BONUS #3: High Converting Webinars Case Studies BONUS #4: Our Trello Webinar Checklist All of this for only £29.99 for a limited period of time. Click here to download. https://webinarconversionkit.com/ Today I have the pleasure to Interview Jaz Ampaw Farr  Jaz has a unique ability to help people and corporations reframe their stories and situations, bringing transformation to their work, life and communities. This is Jaz's alchemy. Multi-award winning keynote speaker and three-time Speaker Of The Year, Jaz's feet are firmly on the ground by her impressive failure CV – especially when it comes to appearing on reality TV shows, which she does once every 20 years! Jaz is highly recognised for the impact she's had on the education and health sectors, galvanising and inspiring those around her. She has led national and international projects, unlocking potential in leaders and teams, and is now highly sought after by organisations worldwide. Connect with Jaz Ampaw Farr   Website:  https://jazampawfarr.com Website:  https://behumanfirst.co Facebook:  https://www.facebook.com/groups/theimpactlounge Instagram: https://www.instagram.com/jazampawfarr LinkedIn: https://www.linkedin.com/in/jazampawfarr TikTok:  https://www.tiktok.com/@jazampawfarr Twitter: https://twitter.com/jazampawfarr YouTube:  https://www.youtube.com/c/JazAmpawFarr/videos To become a GTeX Member, Apply here: https://gtex.events/call  ------- To receive daily support in your coaching and speaking business, join our private Facebook Group EXPLODE YOUR EXPERT BIZ https://www.facebook.com/groups/explodeyourexpertbiz/ ------- Take a full business assessment for free to have absolute clarity on your business with the EXPERT BIZ CHECKLIST. http://bit.ly/expert-biz-checklist-podcast ------ Also, make sure you subscribe to the podcast so you don't miss any other episode.   If you want to reach out to me with your questions, you can email me at Simone@gtex.org.uk that comes right to my inbox. --- Send in a voice message: https://podcasters.spotify.com/pod/show/explode/message

Welcome to another episode of Expert To Authority Show, brought to you by http://gtex.org.uk/,  I am your host, Simone Vincenzi, The Experts Strategist, and this is the podcast for experts who want to become the ultimate authority in their niche while making an impact in the world. We have created the Webinar Conversion Kit where you will get access to: The High-Converting Webinar Framework BONUS #1: High-Converting Webinar Slide Template BONUS #2: Pitch and Follow Up Templates BONUS #3: High Converting Webinars Case Studies BONUS #4: Our Trello Webinar Checklist All of this for only £29.99 for a limited period of time. Click here to download. https://webinarconversionkit.com/ Today I have the pleasure to Interview Dave Bricker  As a young man, Dave Bricker was inspired by the remarkable people he met in Miami's “secret floating village.” The sailboat anchorage a quarter-mile off-shore from Miami City Hall attracted world travelers, squatters, dreamers, and bums. All had remarkable stories to tell. By the time he graduated college, he was living aboard his own tiny sailboat. Soon after graduation, he set sail for the Bahamas with a locker full of food and dreams … and $40 in his pocket. His voyages took him up and down the Bahamas, up the east coast of the US to Chesapeake Bay, and across the Atlantic to Gibraltar. He ran aground, dealt with mechanical breakdowns, got seasick more than once, slept in a volcano, survived powerful storms, and returned to the land of clocks and calendars with what he'd gone in search of—stories of his own. Today, as a speaker, trainer, and coach, he helps remarkable people tell remarkable stories—through writing, speaking, graphic design, video, technology, and music. If you want to say it, share it, or sell it, bring Dave Bricker your story; he'll help you tell it. Connect with Dave Bricker  Website:  https://straighttalkspeakers.com   To become a GTeX Member, Apply here: https://gtex.events/call  ------- To receive daily support in your coaching and speaking business, join our private Facebook Group EXPLODE YOUR EXPERT BIZ https://www.facebook.com/groups/explodeyourexpertbiz/ ------- Take a full business assessment for free to have absolute clarity on your business with the EXPERT BIZ CHECKLIST. http://bit.ly/expert-biz-checklist-podcast ------ Also, make sure you subscribe to the podcast so you don't miss any other episode.   If you want to reach out to me with your questions, you can email me at Simone@gtex.org.uk that comes right to my inbox. --- Send in a voice message: https://podcasters.spotify.com/pod/show/explode/message

BUFFALO, NY- January 30, 2024 – A new #research paper was #published in Aging (listed by MEDLINE/PubMed as "Aging (Albany NY)" and "Aging-US" by Web of Science) Volume 16, Issue 1, entitled, “XRCC1: a potential prognostic and immunological biomarker in LGG based on systematic pan-cancer analysis.” X-ray repair cross-complementation group 1 (XRCC1) is a pivotal contributor to base excision repair, and its dysregulation has been implicated in the oncogenicity of various human malignancies. However, a comprehensive pan-cancer analysis investigating the prognostic value, immunological functions, and epigenetic associations of XRCC1 remains lacking. In this new study, researchers Guobing Wang, Yunyue Li, Rui Pan, Xisheng Yin, Congchao Jia, Yuchen She, Luling Huang, Guanhu Yang, Hao Chi, and Gang Tian from Southwest Medical University, The Affiliated Hospital of Southwest Medical University, Yibin Hospital of T.C.M, Medical School of Nanchang University, Fourth Military Medical University, and Ohio University aimed to address this knowledge gap by conducting a systematic investigation employing bioinformatics techniques across 33 cancer types. “Our analysis encompassed XRCC1 expression levels, prognostic and diagnostic implications, epigenetic profiles, immune and molecular subtypes, Tumor Mutation Burden (TMB), Microsatellite Instability (MSI), immune checkpoints, and immune infiltration, leveraging data from TCGA, GTEx, CELL, Human Protein Atlas, Ualcan, and cBioPortal databases.” Notably, XRCC1 displayed both positive and negative correlations with prognosis across different tumors. Epigenetic analysis revealed associations between XRCC1 expression and DNA methylation patterns in 10 cancer types, as well as enhanced phosphorylation. Furthermore, XRCC1 expression demonstrated associations with TMB and MSI in the majority of tumors. Interestingly, XRCC1 gene expression exhibited a negative correlation with immune cell infiltration levels, except for a positive correlation with M1 and M2 macrophages and monocytes in most cancers. Additionally, the researchers observed significant correlations between XRCC1 and immune checkpoint gene expression levels. Lastly, their findings implicated XRCC1 in DNA replication and repair processes, shedding light on the precise mechanisms underlying its oncogenic effects. “Overall, our study highlights the potential of XRCC1 as a prognostic and immunological pan-cancer biomarker, thereby offering a novel target for tumor immunotherapy." DOI - https://doi.org/10.18632/aging.205426 Corresponding authors - Guanhu Yang - guanhuyang@gmail.com, Hao Chi - Chihao7511@163.com, and Gang Tian - tiangang@swmu.edu.cn Subscribe for free publication alerts from Aging - https://www.aging-us.com/subscribe-to-toc-alerts Keywords - aging, X-ray repair cross-complementation group 1, pan-cancer, prognosis, immune infiltration, tumor microenvironment About Aging-US Launched in 2009, Aging-US publishes papers of general interest and biological significance in all fields of aging research and age-related diseases, including cancer—and now, with a special focus on COVID-19 vulnerability as an age-dependent syndrome. Topics in Aging-US go beyond traditional gerontology, including, but not limited to, cellular and molecular biology, human age-related diseases, pathology in model organisms, signal transduction pathways (e.g., p53, sirtuins, and PI-3K/AKT/mTOR, among others), and approaches to modulating these signaling pathways. Visit our website at https://www.Aging-US.com​​ and connect with us: Facebook - https://www.facebook.com/AgingUS/ X - https://twitter.com/AgingJrnl Instagram - https://www.instagram.com/agingjrnl/ YouTube - https://www.youtube.com/@AgingJournal LinkedIn - https://www.linkedin.com/company/aging/ Pinterest - https://www.pinterest.com/AgingUS/ Spotify - https://open.spotify.com/show/1X4HQQgegjReaf6Mozn6Mc MEDIA@IMPACTJOURNALS.COM

Hear the inspiring story of how an Italian immigrant to the UK worked his way up from working in restaurants to being a successful online entrepreneur. Simone Vincenzi shares tips and advice on starting an online business, transitioning from one industry to another, and achieving both financial and location independence as an expat.   You'll hear about Simone's favorite places to travel, the quickest way to learn English as a second language (as an adult), and the shocking racism he experienced while traveling with his wife in South Africa. Plus, he tells you the benefits of creating a webinar for your business and how to create one on a tight budget.   Simone Vincenzi is a multi-award winning serial entrepreneur and author who has helped people to launch more than 500 profitable businesses before the age of 30 with his company GTeX. Tune in to learn more!   Special Offers:  Travel with Kristin to Portugal in 2024 Get The Webinar Conversion Kit for only $30 (half of the money is donated to a basketball charity) Try Bonsai, a comprehensive tool for your business. Apply for Relocation Support from Kristin Open a Wise Borderless Account and get a free transfer up to €500   Topics Discussed: Moving to a new country as a young adult Learning English as a second language (the quickest way) The food scene in England vs. Italy Why Simone fell in love with England Transitioning to a new industry Public speaking Making an income online How to start traveling (even when it seems impossible) Culture, food/wine, travel tips, and racism in South Africa Aligning travel with business opportunities How Simone trains people to have financial and location independence The benefits of creating a webinar for your business   Questions Answered: How did you move from Italy to the UK? What was the hardest part about adapting to UK culture? How do you learn English more quickly? What skills do you need to make money online? Did you feel safe traveling in South Africa? What business problem are you trying to solve right now? and more!   Episode Resources:    Recommended Videos: Top 10 Best Places To Live Well on $1,000 Per Month 8 Reasons I DON'T MISS Living in the USA I Asked Digital Nomads How They Make Money Living On A Remote Island

Guest: Simone Vincenzi   Guest Bio: Simone Vincenzi, known as The Experts Strategist, is the co-founder of GTeX, Forbes, and Huffington Post contributor, TEDx speaker, Host of the podcast Explode Your Expert Business, and author of 3 life-changing books. Simone is passionate about turning experts into authorities using his signature EXPLODE YOUR EXPERT BIZ programs. Every year he speaks in front of more than 5,000 business leaders, which includes sharing the stage with Les Brown, Dr John DeMartini, Trent Shelton, and many more. He has also organized over 170 events, including Gary Vee speaking from the York Hall boxing ring, and he is currently speaking on more than 200 stages every year. Following his passion for making an impact on the younger generation, he also partners with the largest youth organizations in the UK, helping them deliver more effective courses and training. Simone cannot live without 3 things: Speaking, basketball, and playing his Didgeridoo.   Guest Links: https://webinarconversionkit.com/cww   Simone's Ted Talk with Didgerido: https://youtu.be/ay9ZBGviluA     About Salesology®: Conversations with Sales Leaders Download your free gift, The Salesology® Vault. The vault is packed full of free gifts from sales leaders, sales experts, marketing gurus and revenue generation experts.  Download your free gift, 81 Tools to Grow Your Sales & Your Business Faster, More Easily & More Profitably. Save hours of work tracking down the right prospecting and sales resources and/or digital tools that every business owner and salesperson needs. Watch the demo of the Salesology® Prospecting Method, A Simple, 3-Step Method That, On Average, Increases Qualified Appointments & Sales By 73%. If you are a business owner or sales manager with an underperforming sales team, let's talk. Click here to schedule a time. Please, subscribe to Salesology®: Conversations with Sales Leaders so that you don't miss a single episode, and while you're at it, won't you take a moment to write a short review and rate our show? It would be greatly appreciated! To learn more about our previous guests, listen to past episodes, and get to know your host, go to https://podcast.gosalesology.com/ and connect on LinkedIn and follow us on Facebook and Twitter and checkout our website at  http://www.gosalesology.com

Get in contact with Simone Vincenzi simone@gtex.org.uk GTex: https://gtex.org.uk/ TEDX Talks Meet Simone Vincenzi, a multi-award-winning serial entrepreneur and author who's transformed over 500 startups into profitable ventures by the age of 30 through his company GTeX. Simone's mission is to empower community-driven businesses, where members shape decisions and fuel creative ideas. TEDX Talks Recognized as one of the UK's influential migrant entrepreneurs in the House of Parliament, Simone contributes to Forbes and Entrepreneur Magazine while appearing on various TV and radio shows. He guides startup entrepreneurs, industry experts, and multi-millionaires in realizing their business dreams swiftly. Simone is a captivating, hands-on speaker who blends music, magic, and basketball to infuse fun into his knowledge-packed seminars. He's shared the stage with luminaries like #lesbrown, #tonyrobbins,, and #garyvee #TedX #Talks Join us to explore Simone's journey, from business success to giving back to the entrepreneurial community.

Welcome to another episode of Expert To Authority Show, brought to you by http://gtex.org.uk/,  I am your host, Simone Vincenzi, The Experts Strategist, and this is the podcast for experts who want to become the ultimate authority in their niche while making an impact in the world. We have created the Webinar Conversion Kit where you will get access to: The High-Converting Webinar Framework BONUS #1: High-Converting Webinar Slide Template BONUS #2: Pitch and Follow Up Templates BONUS #3: High Converting Webinars Case Studies BONUS #4: Our Trello Webinar Checklist All of this for only £29.99 for a limited period of time. Click here to download. https://webinarconversionkit.com/ Today I have the pleasure to Interview Jane McLelland As a long term stage 4 survivor, Jane mission is to empower other cancer patients to understand the abnormal cancer metabolism so they too can starve their cancer. This can be achieved with a combination of off label drugs, key supplements and a personalised diet. My second edition of How To Starve Cancer (published July 2021) and her online course includes how cancer can be killed by ferroptosis, 'death by iron'. Jane worked for many years as a Chartered Physiotherapist, then entrepreneur, winning Gift of the Year 2003 with my natural BATHrobics toiletries and home spa rituals. These were sold nationwide through major department stores (eg John Lewis, Debenhams, House of Fraser) and chemists. Connect with Jane McLelland Linkedin:  https://www.linkedin.com/in/jane-mclelland-33b4774b/ Website:  https://www.howtostarvecancer.com/ Website: https://how-to-starve-cancer.teachable.com/p/how-to-starve-cancer-online-course   To become a GTeX Member, Apply here: https://gtex.events/call  ------- To receive daily support in your coaching and speaking business, join our private Facebook Group EXPLODE YOUR EXPERT BIZ https://www.facebook.com/groups/explodeyourexpertbiz/ ------- Take a full business assessment for free to have absolute clarity on your business with the EXPERT BIZ CHECKLIST. http://bit.ly/expert-biz-checklist-podcast ------ Also, make sure you subscribe to the podcast so you don't miss any other episode.   If you want to reach out to me with your questions, you can email me at Simone@gtex.org.uk that comes right to my inbox --- Send in a voice message: https://podcasters.spotify.com/pod/show/explode/message

Welcome to another episode of Expert To Authority Show, brought to you by http://gtex.org.uk/,  I am your host, Simone Vincenzi, The Experts Strategist, and this is the podcast for experts who want to become the ultimate authority in their niche while making an impact in the world. We have created the Webinar Conversion Kit where you will get access to: The High-Converting Webinar Framework BONUS #1: High-Converting Webinar Slide Template BONUS #2: Pitch and Follow Up Templates BONUS #3: High Converting Webinars Case Studies BONUS #4: Our Trello Webinar Checklist All of this for only £29.99 for a limited period of time. Click here to download. https://webinarconversionkit.com/ Today I have the pleasure to Interview Caroline Sylvia Wragg Caroline is an Award winning international marketing and Sales strategist and coach, Experienced consultant, Speaker, Director and business wellbeing mentor. She help business owners and companies who are passionate about what they do create sustainable business growth through strategic marketing and sales. Building the right relationships is vital to success and help to have a VOICE connecting you with the right clients, getting seen and heard. Increased Productivity, accelerated sales and business growth are some of the results clients are getting through our bespoke business systems and marketing consultancy programmes. Connecting businesses with the right clients, raising profile and expanding reach is something that takes time in business and understanding how this works is key. Helping people has always been her passion that's why she help businesses with excellent results driven marketing from many years' experience and combining it with strong communications and sales that works.   Connect with Caroline Sylvia Wragg Linkedin:  https://www.linkedin.com/in/carolinesylviawragg/   To become a GTeX Member, Apply here: https://gtex.events/call  ------- To receive daily support in your coaching and speaking business, join our private Facebook Group EXPLODE YOUR EXPERT BIZ https://www.facebook.com/groups/explodeyourexpertbiz/ ------- Take a full business assessment for free to have absolute clarity on your business with the EXPERT BIZ CHECKLIST. http://bit.ly/expert-biz-checklist-podcast ------ Also, make sure you subscribe to the podcast so you don't miss any other episode.   If you want to reach out to me with your questions, you can email me at Simone@gtex.org.uk that comes right to my inbox. --- Send in a voice message: https://podcasters.spotify.com/pod/show/explode/message

Welcome to another episode of Expert To Authority Show, brought to you by http://gtex.org.uk/,  I am your host, Simone Vincenzi, The Experts Strategist, and this is the podcast for experts who want to become the ultimate authority in their niche while making an impact in the world. We have created the Webinar Conversion Kit where you will get access to: The High-Converting Webinar Framework BONUS #1: High-Converting Webinar Slide Template BONUS #2: Pitch and Follow Up Templates BONUS #3: High Converting Webinars Case Studies BONUS #4: Our Trello Webinar Checklist All of this for only £29.99 for a limited period of time. Click here to download. https://webinarconversionkit.com/ Today I have the pleasure to Interview Vishal Shah Vishal is known as the Subconscious Mind Communicator. He empowers individuals by helping them connect to their subconscious, in the process healing their pain, their relationships and gaining clarity on their purpose in life. He is an experienced trainer with over 7 years of experience in the self and leadership development industry.  Having worked with organisations both medium and large he has the skills necessary to motivate and work with both individuals and groups.  Vishal's approach is an inside out approach. He helps you to access your unconscious / subconscious mind, releasing or integrating emotions and traumas that have been sabotaging your life, connecting you to your intuition and helping you seek within. Connect with Vishal Shah Facebook:  https://www.facebook.com/groups/live-with-mindfulness   To become a GTeX Member, Apply here: https://gtex.events/call  ------- To receive daily support in your coaching and speaking business, join our private Facebook Group EXPLODE YOUR EXPERT BIZ https://www.facebook.com/groups/explodeyourexpertbiz/ ------- Take a full business assessment for free to have absolute clarity on your business with the EXPERT BIZ CHECKLIST. http://bit.ly/expert-biz-checklist-podcast ------ Also, make sure you subscribe to the podcast so you don't miss any other episode.   If you want to reach out to me with your questions, you can email me at Simone@gtex.org.uk that comes right to my inbox. --- Send in a voice message: https://podcasters.spotify.com/pod/show/explode/message

Welcome to another episode of Expert To Authority Show, brought to you by http://gtex.org.uk/,  I am your host, Simone Vincenzi, The Experts Strategist, and this is the podcast for experts who want to become the ultimate authority in their niche while making an impact in the world. We have created the Webinar Conversion Kit where you will get access to: The High-Converting Webinar Framework BONUS #1: High-Converting Webinar Slide Template BONUS #2: Pitch and Follow Up Templates BONUS #3: High Converting Webinars Case Studies BONUS #4: Our Trello Webinar Checklist All of this for only £29.99 for a limited period of time. Click here to download. https://webinarconversionkit.com/ Today I have the pleasure to Interview Jennifer Darling Think “force of nature” – in the best possible way. A Revenue Generating Machine and In-Demand Speaker, Jennifer Darling has a knack for turning ideas into money-making opportunities and helping leaders expand their professional brands online.  She teaches audiences to engage innovatively, connect more effectively, and increase their visibility in new and valuable ways that lead to more customers and referrals.With over 20 years of experience in advertising sales and sales management for companies such as FOX, NBC, CBS, and Comcast, she knows what works and what doesn't to boost sales. Even in the most challenging times, she's led her team to record-breaking results. In this episode GTeX Member Of The Month July 2023 - Jennifer Darling discuss about how she got $27,000 with One Webinar Connect with Jennifer Darling Linkedin:  https://www.linkedin.com/in/jenniferdarlingspeaks/ Twitter:  therealjdarling Website: https://jenniferdarlingspeaks.com/   To become a GTeX Member, Apply here: https://gtex.events/call  ------- To receive daily support in your coaching and speaking business, join our private Facebook Group EXPLODE YOUR EXPERT BIZ https://www.facebook.com/groups/explodeyourexpertbiz/ ------- Take a full business assessment for free to have absolute clarity on your business with the EXPERT BIZ CHECKLIST. http://bit.ly/expert-biz-checklist-podcast ------ Also, make sure you subscribe to the podcast so you don't miss any other episode.   If you want to reach out to me with your questions, you can email me at Simone@gtex.org.uk that comes right to my inbox. --- Send in a voice message: https://podcasters.spotify.com/pod/show/explode/message

Welcome to another episode of Expert To Authority Show, brought to you by http://gtex.org.uk/,  I am your host, Simone Vincenzi, The Experts Strategist, and this is the podcast for experts who want to become the ultimate authority in their niche while making an impact in the world. We have created the Webinar Conversion Kit where you will get access to: The High-Converting Webinar Framework BONUS #1: High-Converting Webinar Slide Template BONUS #2: Pitch and Follow Up Templates BONUS #3: High Converting Webinars Case Studies BONUS #4: Our Trello Webinar Checklist All of this for only £29.99 for a limited period of time. Click here to download. https://webinarconversionkit.com/ Today I have the pleasure to Interview Marcel Wijermars Marcel brings amazing entrepreneurs together through cureated dinners and masterclasses. His background as an entrepreneur, combined with his event organization experience in the United States, Mexico, and Bali, enables him to craft Amsterdam's premier entrepreneur community. Preferring genuine connection over transactional and sales-oriented networking events, he named the entrepreneur community: No More Networking. The No More Networking community attracts a diverse group of ambitious entrepreneurs who recognize the tremendous value in surrounding themselves with individuals who bring out their best selves and provide an abundance of opportunities. In this episode GTeX Member Of The Month June 2023 - Marcel Wijermars discuss about how he built a global community in months Connect with Marcel Wijermars Linkedin:  https://www.linkedin.com/in/marcelwijermars/ Instagram: https://www.instagram.com/marcelwijermars/ Facebook: https://www.facebook.com/marcel.wijermars   To become a GTeX Member, Apply here: https://gtex.events/call  ------- To receive daily support in your coaching and speaking business, join our private Facebook Group EXPLODE YOUR EXPERT BIZ https://www.facebook.com/groups/explodeyourexpertbiz/ ------- Take a full business assessment for free to have absolute clarity on your business with the EXPERT BIZ CHECKLIST. http://bit.ly/expert-biz-checklist-podcast ------ Also, make sure you subscribe to the podcast so you don't miss any other episode.   If you want to reach out to me with your questions, you can email me at Simone@gtex.org.uk that comes right to my inbox. --- Send in a voice message: https://podcasters.spotify.com/pod/show/explode/message

Welcome to another episode of Expert To Authority Show, brought to you by http://gtex.org.uk/,  I am your host, Simone Vincenzi, The Experts Strategist, and this is the podcast for experts who want to become the ultimate authority in their niche while making an impact in the world. We have created the Webinar Conversion Kit where you will get access to: The High-Converting Webinar Framework BONUS #1: High-Converting Webinar Slide Template BONUS #2: Pitch and Follow Up Templates BONUS #3: High Converting Webinars Case Studies BONUS #4: Our Trello Webinar Checklist All of this for only £29.99 for a limited period of time. Click here to download. https://webinarconversionkit.com/ Today I have the pleasure to Interview Ben Chai Ben passions are in education, media, technology, property and mentoring others to build successful lives and businesses. Companies and individuals have benefited from his cross industry experience using out of the box thinking and applying strategies used in other industries to build their own success road map. Businesses have greatly increased growth from my connections, tactical and strategic ability combined with my knowledge of how to create engaging media content. This content is visible when an online search on Ben Chai is made. Connect with Ben Chai Website:  https://www.social-magnetism.com TEDx Talk:  https://youtu.be/y0tVnE-8mwU Instagram: https://www.instagram.com/benchaiofficial/ To become a GTeX Member, Apply here: https://gtex.events/call  ------- To receive daily support in your coaching and speaking business, join our private Facebook Group EXPLODE YOUR EXPERT BIZ https://www.facebook.com/groups/explodeyourexpertbiz/ ------- Take a full business assessment for free to have absolute clarity on your business with the EXPERT BIZ CHECKLIST. http://bit.ly/expert-biz-checklist-podcast ------ Also, make sure you subscribe to the podcast so you don't miss any other episode.   If you want to reach out to me with your questions, you can email me at Simone@gtex.org.uk that comes right to my inbox. --- Send in a voice message: https://podcasters.spotify.com/pod/show/explode/message

Welcome to another episode of Expert To Authority Show, brought to you by http://gtex.org.uk/,  I am your host, Simone Vincenzi, The Experts Strategist, and this is the podcast for experts who want to become the ultimate authority in their niche while making an impact in the world. We have created the Webinar Conversion Kit where you will get access to: The High-Converting Webinar Framework BONUS #1: High-Converting Webinar Slide Template BONUS #2: Pitch and Follow Up Templates BONUS #3: High Converting Webinars Case Studies BONUS #4: Our Trello Webinar Checklist All of this for only £29.99 for a limited period of time. Click here to download. https://webinarconversionkit.com/ Today I have the pleasure to Interview Carol Anne Cooper Carol Anne assists women to shine, by getting their story written. She is creator and leader of the M.O.V.E. Method writing programme ‘Let's Write', developed through her own journey from Maths teacher to becoming The Writing Facilitator, discovering tools and techniques that have assisted her in unlocking the writer within. Connect with Carol Anne Cooper Linkedin:  https://www.linkedin.com/in/carolannecooper Facebook:  https://www.facebook.com/carolanne.cooper.96 Facebook Group: https://www.facebook.com/groups/GetYourBookWritten Instagram:  https://www.instagram.com/carol.anne.writing.facilitator To become a GTeX Member, Apply here: https://gtex.events/call  ------- To receive daily support in your coaching and speaking business, join our private Facebook Group EXPLODE YOUR EXPERT BIZ https://www.facebook.com/groups/explodeyourexpertbiz/ ------- Take a full business assessment for free to have absolute clarity on your business with the EXPERT BIZ CHECKLIST. http://bit.ly/expert-biz-checklist-podcast ------ Also, make sure you subscribe to the podcast so you don't miss any other episode.   If you want to reach out to me with your questions, you can email me at Simone@gtex.org.uk that comes right to my inbox. --- Send in a voice message: https://podcasters.spotify.com/pod/show/explode/message

Welcome to another episode of Expert To Authority Show, brought to you by http://gtex.org.uk/,  I am your host, Simone Vincenzi, The Experts Strategist, and this is the podcast for experts who want to become the ultimate authority in their niche while making an impact in the world. We have created the Webinar Conversion Kit where you will get access to: The High-Converting Webinar Framework BONUS #1: High-Converting Webinar Slide Template BONUS #2: Pitch and Follow Up Templates BONUS #3: High Converting Webinars Case Studies BONUS #4: Our Trello Webinar Checklist All of this for only £29.99 for a limited period of time. Click here to download. https://webinarconversionkit.com/ Today I have the pleasure to Interview Angela Roth After she developed her virtual coaching business during the Covid years, Angela Roth discovered that many heart-led coaches were struggling to build successful businesses on their own. Hearing their stories filled her with compassion, so she created a new Community-based business, Succeed From The Start, where they could thrive together! Connect with Angela Roth Linkedin: https://www.linkedin.com/in/angela-roth-succeed-from-the-start/ Facebook: https://www.facebook.com/groups/steps4success/  Website: www.succeedfromthestart.com To become a GTeX Member, Apply here: https://gtex.events/call  ------- To receive daily support in your coaching and speaking business, join our private Facebook Group EXPLODE YOUR EXPERT BIZ https://www.facebook.com/groups/explodeyourexpertbiz/ ------- Take a full business assessment for free to have absolute clarity on your business with the EXPERT BIZ CHECKLIST. http://bit.ly/expert-biz-checklist-podcast ------ Also, make sure you subscribe to the podcast so you don't miss any other episode.   If you want to reach out to me with your questions, you can email me at Simone@gtex.org.uk that comes right to my inbox. --- Send in a voice message: https://podcasters.spotify.com/pod/show/explode/message

Welcome to another episode of Expert To Authority Show, brought to you by http://gtex.org.uk/, I am your host, Simone Vincenzi, The Experts Strategist, and this is the podcast for experts who want to become the ultimate authority in their niche while making an impact in the world. We have created the Webinar Conversion Kit where you will get access to: The High-Converting Webinar Framework BONUS #1: High-Converting Webinar Slide Template BONUS #2: Pitch and Follow Up Templates BONUS #3: High Converting Webinars Case Studies BONUS #4: Our Trello Webinar Checklist All of this for only £29.99 for a limited period of time. Click here to download. https://webinarconversionkit.com/ Today I have the pleasure to Interview Marjorie Gutierrez Marjorie (Gutierrez) is a Systems Strategist with over 25 years of experience working in the Ecological Travel Business and Fine Art Shipping Industry between London and New York. Today, small business owners and entrepreneurs hire here to double their revenue within 6 months by implementing structures and processes that work. Connect with Marjorie Gutierrez Linkedin: https://linkedin.com/in/marjorie-gutierrez Facebook: https://www.facebook.com/marjorie.gutierrez.562 Instagram: https://instagram.com/marjorie.gutierrez_ To become a GTeX Member, Apply here: https://gtex.events/call ------- To receive daily support in your coaching and speaking business, join our private Facebook Group EXPLODE YOUR EXPERT BIZ https://www.facebook.com/groups/explodeyourexpertbiz/ ------- Take a full business assessment for free to have absolute clarity on your business with the EXPERT BIZ CHECKLIST. http://bit.ly/expert-biz-checklist-podcast ------ Also, make sure you subscribe to the podcast so you don't miss any other episode. If you want to reach out to me with your questions, you can email me at Simone@gtex.org.uk that comes right to my inbox. --- Send in a voice message: https://anchor.fm/explode/message

Los seres vivos estamos sometidos a ciclos diarios y estacionales que influyen en nuestro metabolismo y comportamiento. La alteración en la expresión de los genes puede beneficiar o tener efectos perjudiciales para la salud humana. Un estudio del Centro Regulación Genómica en el marco del proyecto GTEx ha elaborado una base de datos de esas variaciones en cada tejido e incluye genes que son dianas terapéuticas para el cáncer, que podrían de beneficiarse de los cambios de las dosis del tratamiento según la hora del día o la época del año. Hemos entrevistado a Manuel Irimia, coautor del estudio. El doctor Pedro Gargantilla nos ha hablado del cáncer de colon y de cómo el cantante Pau Dones reflejó como afrontó esta enfermedad en una de sus canciones más íntimas: “Humo”. Alejandro Parrilla nos ha informado de la apertura al público en Luxor de las tumbas de Djehuty y Hery, dos altos funcionarios del Egipto faraónico, excavadas y restauradas desde 2002 por el Proyecto Djehuty, liderado por el CSIC. Con testimonios del arqueólogo José Manuel Galán. Carlos Briones nos ha contado como la investigación básica en astrobiología con el desarrollo de sensores para la búsqueda de vida en otros mundos, tiene aplicaciones en el nuestro para la detección de virus como el de la hepatitis C. Jesús Pérez Gil nos ha hablado de los surfactantes, moléculas que facilitan que nuestros pulmones puedan hincharse de aire cada vez que respiramos, y cuya ausencia en los bebés prematuros constituye uno de los principales problemas para los niños que nacen antes de tiempo. Con Jesús Zamora hemos analizado como una vejez más saludable ha cambiado la calidad y los hábitos de vida y donde antes se hablaba de cincuentones, por ejemplo, ahora se habla de cincuentañeros. Escuchar audio

Welcome to another episode of Expert To Authority Show, brought to you by http://gtex.org.uk/, I am your host, Simone Vincenzi, The Experts Strategist, and this is the podcast for experts who want to become the ultimate authority in their niche while making an impact in the world. We have created the Webinar Conversion Kit where you will get access to: The High-Converting Webinar Framework BONUS #1: High-Converting Webinar Slide Template BONUS #2: Pitch and Follow Up Templates BONUS #3: High Converting Webinars Case Studies BONUS #4: Our Trello Webinar Checklist All of this for only £29.99 for a limited period of time. Click here to download. https://webinarconversionkit.com/ Today I have the pleasure to Interview Ian Philip James Ian love helping people and teams to solve tricky, thorny and complex problems by imaginatively and curiously untangling them bit by bit. His super powers are asking powerful questions that unlock thinking and helping people to identify and cut straight to the point of an issue. He worked in all sorts of places and industries, local authority, theatre, private training, housing (real estate and social), private facilitation, entertainment. He worked for all sorts of people… Hackney Council, Newham Council, London Pantomimers, GTEX, MyLondonHome, Circle Housing and private clients include… BBC, New Look, Mood Media, Sir Paul McCartney, Vinopolis as well as a number of private individuals. Connect with Ian Philip James Linkedin: https://www.linkedin.com/in/ian-philip-james-886a1b38/ Facebook: https://www.facebook.com/ian.james.357 To become a GTeX Member, Apply here: https://gtex.events/call ------- To receive daily support in your coaching and speaking business, join our private Facebook Group EXPLODE YOUR EXPERT BIZ https://www.facebook.com/groups/explodeyourexpertbiz/ ------- Take a full business assessment for free to have absolute clarity on your business with the EXPERT BIZ CHECKLIST. http://bit.ly/expert-biz-checklist-podcast ------ Also, make sure you subscribe to the podcast so you don't miss any other episode. If you want to reach out to me with your questions, you can email me at Simone@gtex.org.uk that comes right to my inbox. --- Send in a voice message: https://anchor.fm/explode/message

Welcome to another episode of Expert To Authority Show, brought to you by http://gtex.org.uk/, I am your host, Simone Vincenzi, The Experts Strategist, and this is the podcast for experts who want to become the ultimate authority in their niche while making an impact in the world. We have created the Webinar Conversion Kit where you will get access to: The High-Converting Webinar Framework BONUS #1: High-Converting Webinar Slide Template BONUS #2: Pitch and Follow Up Templates BONUS #3: High Converting Webinars Case Studies BONUS #4: Our Trello Webinar Checklist All of this for only £29.99 for a limited period of time. Click here to download. https://webinarconversionkit.com/ Today I have the pleasure to Interview Patricia Soares Paty help creative entrepreneurs make quicker and smarter business decisions that lead them to their next level. She do that by being their strategic sounding board and giving them the roadmap and steps necessary to get there and make their vision a reality. Connect with Patricia Website: https://patysoares.com/ Instagram: https://www.instagram.com/iampatysoares/ Facebook: https://www.facebook.com/Patydesign To become a GTeX Member, Apply here: https://gtex.events/call ------- To receive daily support in your coaching and speaking business, join our private Facebook Group EXPLODE YOUR EXPERT BIZ https://www.facebook.com/groups/explodeyourexpertbiz/ ------- Take a full business assessment for free to have absolute clarity on your business with the EXPERT BIZ CHECKLIST. http://bit.ly/expert-biz-checklist-podcast ------ Also, make sure you subscribe to the podcast so you don't miss any other episode. If you want to reach out to me with your questions, you can email me at Simone@gtex.org.uk that comes right to my inbox. --- Send in a voice message: https://anchor.fm/explode/message

Business is a team sport, and the sooner one embraces it by developing better culture, the sooner the organizational goals can be realized. In this episode, Jerome interviews Simone about his journey in starting and growing GTeX, a company focused on exponential growth through events. Simone shares his story of overcoming challenges and growing his business to over 200 events per year. Listen in!   [00:01 - 15:35] Scaling an Events Business Exponentially The role of events in connecting people and creating opportunities The more people you know, the more opportunities you will have Simone discusses the challenges of running an event business  [15:36 - 33:09] Teamwork Makes the Dream Work Successful in the event business - start small to go big Changes in brand perception when achieving big-ticket clients The importance of association - with organizations, people, and charities [33:10 - 47:33] Closing Segment Business is a team sport - embrace it Connect with Simone! Closing words   Tweetable Quote: “Be the kind of person who gives first. Be the first person who helps out, who helps others… Just be nice, be a good human being…that helps a lot in life.”  - Simone Vincenzi Connect with Simone: Linkedin: https://www.linkedin.com/in/simone-vincenzi/  Website: https://gtex.org.uk/  Resources Mentioned Influence: The Psychology of Persuasion by Robert B. Cialdini   LEAVE A REVIEW + help someone who wants to explode their business growth by sharing this episode or click here to listen to our previous episodes.   Dreamcatchers is an inclusive organization that targets people's interest in being more instead of a certain demographic. We have people from all walks of life at many different ages. Find out more at www.dreamsshouldbereal.com. Find out more about Jerome at www.d3v3loping.com or www.myersmethods.com. Learn more about your ad choices. Visit megaphone.fm/adchoices

Welcome to another episode of Expert To Authority Show, brought to you by http://gtex.org.uk/, I am your host, Simone Vincenzi, The Experts Strategist, and this is the podcast for experts who want to become the ultimate authority in their niche while making an impact in the world. We have created the Webinar Conversion Kit where you will get access to: The High-Converting Webinar Framework BONUS #1: High-Converting Webinar Slide Template BONUS #2: Pitch and Follow Up Templates BONUS #3: High Converting Webinars Case Studies BONUS #4: Our Trello Webinar Checklist All of this for only £29.99 for a limited period of time. Click here to download. https://webinarconversionkit.com/ Today I have the pleasure to Interview Jelena Radonjic Jelena is an award-winning Career Fulfilment and Leadership Development Coach, speaker, author and Forbes Coaches Council contributor. She is passionate about helping conscious, aspiring professionals thrive in the careers they love. With 25 years in international recruitment and education management and CTI coaching qualification, she brings together unique insights into how recruiters think and how personal coaches work. Jelena has lived and worked in 3 countries, speaks 4 languages and is passionate about cross-cultural competence and communication. Working in managerial roles with P&L responsibility, sales, business development, candidate selection and talent acquisition, from start-ups to University Career Service, to growing SMEs in Japan and the UK, Jelena has developed a strong business acumen and commercial awareness that allow her to coach clients from a variety of industries and seniority levels. She has also worked with spiritual teachers such as John DeMartini and Deepak Chopra and infuses love, joy and spirit into her coaching and everything she does. Through WhatWork, her career coaching consultancy, she offers individual and group coaching for Career Transitions and Career Development. She often speaks at events on Career Fulfilment, How to Make Conscious Career Decisions, Effortless Networking, Interview Skills for the 21st Century, to name a few. Connect with Jelena Website: https://www.whatwork.co.uk/ Linkedin: https://www.facebook.com/jradonjic1 Twitter: https://twitter.com/whatworkuk Facebook: https://www.facebook.com/groups/ALLTHINGSCAREER/ To become a GTeX Member, Apply here: https://gtex.events/call ------- To receive daily support in your coaching and speaking business, join our private Facebook Group EXPLODE YOUR EXPERT BIZ https://www.facebook.com/groups/explodeyourexpertbiz/ ------- Take a full business assessment for free to have absolute clarity on your business with the EXPERT BIZ CHECKLIST. http://bit.ly/expert-biz-checklist-podcast ------ Also, make sure you subscribe to the podcast so you don't miss any other episode. If you want to reach out to me with your questions, you can email me at Simone@gtex.org.uk that comes right to my inbox. --- Send in a voice message: https://anchor.fm/explode/message

Welcome to another episode of Expert To Authority Show, brought to you by http://gtex.org.uk/, I am your host, Simone Vincenzi, The Experts Strategist, and this is the podcast for experts who want to become the ultimate authority in their niche while making an impact in the world. We have created the Webinar Conversion Kit where you will get access to: The High-Converting Webinar Framework BONUS #1: High-Converting Webinar Slide Template BONUS #2: Pitch and Follow Up Templates BONUS #3: High Converting Webinars Case Studies BONUS #4: Our Trello Webinar Checklist All of this for only £29.99 for a limited period of time. Click here to download. https://webinarconversionkit.com/ In this episode, I interviewed yesterday our GTeX Member of the month Riikka Manninen on the success of her first webinar. She just started out her journey in business, had a very small audience and never sold something from a presentation. So far she made more than 20 sales counting making it the biggest month she ever had. She followed the system we share on the Webinar Conversion Kit. If you want to get clients from webinars and finally make webinars work for you, the Webinar Conversion Kit is for you. Here is the link, check it out. https://webinarconversionkit.com/ Connect with Riikka Facebook: https://www.facebook.com/riikka.manninen.7 Facebook: https://www.facebook.com/hoitavahetki To become a GTeX Member, Apply here: https://gtex.events/call ------- To receive daily support in your coaching and speaking business, join our private Facebook Group EXPLODE YOUR EXPERT BIZ https://www.facebook.com/groups/explodeyourexpertbiz/ ------- Take a full business assessment for free to have absolute clarity on your business with the EXPERT BIZ CHECKLIST. http://bit.ly/expert-biz-checklist-podcast ------ Also, make sure you subscribe to the podcast so you don't miss any other episode. If you want to reach out to me with your questions, you can email me at Simone@gtex.org.uk that comes right to my inbox. --- Send in a voice message: https://anchor.fm/explode/message

Who is Simone?Simone is a multi-award winning serial entrepreneur and author who helped to lunch more than 500 profitable businesses before he was 30 through his company GTeX. He is passionate about building thriving community-led businesses where the members are the core and essence of the decisions and development of ideas.He works with startup entrepreneurs and experts who want to grow their existing business as well as multi-millionaires who want to launch new ventures, bringing ideas to fruition at lightning speedKey Takeaways1. Human beings come together into communities, and o interact in communities. Community-led leaders want to stand out from the crowd and build something meaningful for society2. Too often community-led business leaders cannot build an audience, service customers and build offers all the same time and to do that consistently needs help to develop a way to do it3. If you are a community-led business and care about your 'tribe' you need to care about money and cash flow so that you can stay in business and continue to help themValuable Free Resource or ActionSee getx.org.uk has many free resources, podcast and training courses to helpA video version of this podcast is available on YouTube : _________________________________________________________________________________________________Subscribe to our newsletter and get details of when we are doing these interviews live at https://TCA.fyi/newsletterFind out more about being a guest at : link.thecompleteapproach.co.uk/beaguestSubscribe to the podcast at https://link.thecompleteapproach.co.uk/podcastHelp us get this podcast in front of as many people as possible. Leave a nice five-star review at  apple podcasts : https://link.thecompleteapproach.co.uk/apple-podcasts and on YouTube : https://link.thecompleteapproach.co.uk/Itsnotrocketscienceatyt!Here's how you can bring your business to THE next level:1. Download my free resource on everything you need to grow your business on a single page : https://link.thecompleteapproach.co.uk/1pageIt's a detailed breakdown of how you can grow your business to 7-figures in a smart and sustainable way2. Join The Complete Approach Facebook Group :  https://TCA.fyi/fb Connect with like-minded individuals who are all about growth and increasing revenue. It's a Facebook community where we make regular posts aimed at inspiring conversations in a supportive environment. It's completely free and purposely aimed at expanding and building networks.3. Join our Success to Soar Program and get TIME and FREEDOM. : https://link.thecompleteapproach.co.uk/Success-to-SoarIf you're doing 10-50k a month right now: I'm working with a few business owners like you to change that, without working nights and weekends. If you'd like to get back that Time and still Scale, check the link above.4. Work with me privatelyIf you'd like to work directly with me and my team to take you from 5 figure to 6 and multi 6 figure months, whilst reducing reliance on you. Click on https://link.thecompleteapproach.co.uk/DiscoveryCall  tell me about your business and what you'd like to work on together, and I'll get you all the details.————————————————————————————————————————————-TranscriptNote, this was transcribed using a transcription software and may not reflect the exact words used in the podcast)SUMMARY KEYWORDSbusiness, simone, community, realise, build, work, running, people, book, absolutely, customers, spending, question, client, podcast, purpose, ideas, launch, service based businesses, moneySPEAKERSSimone Vincenzi, Stuart WebbStuart Webb  00:22Hi again and welcome to It's not rocket science. Five questions over coffee. I haven't actually got a coffee today I'm already over caffeinated. So this is just a water but I'm here today with Simone vincenzi. I don't know if you've got a coffee with you, Simone.Simone Vincenzi  00:40I don't I'm I'm over caffeinated as well. Same. The same thing.Stuart Webb  00:44Simone is, is a multi award winning serial entrepreneur and author, he helped to launch more than 500 profitable businesses before he reached the age of 30, which sounds really impressive. His his company is GTEx. And he's passionate about building thriving community led businesses when the when the members are the core and essence of the decisions and development of ideas, Simone, I'm really looking forward to the discussion today. And welcome to the podcastSimone Vincenzi  01:16that thank you for having me here. And I cannot wait to get started. Thank you forStuart Webb  01:20Well, let's start then with my first question which is So describe your your ideal client, the the perfect person that you help with your with your business, Gtech?Simone Vincenzi  01:29Yeah, people that we work with are people that are generally running service based businesses. And what they are, they are very driven by a sense of community, they're very driven by making a difference and better driven by making an impact. And they've got a core expertise or something that they are doing or something and want to be known for. And they want to get their voice out there, they want to get seen, they want to get known. They want to make a difference with their voice, because they realise that they have some skills that can definitely help other people. And, but having a voice and I mean a story or running a business, they are two different they're two different things. Right? So then they stumble across to say, I want to run a business. And they might get a few clients, and then at some point, they get stuck. And so that's the that's where we come in. And that's the kind of people that we work with.Stuart Webb  02:25Yeah, I know, I know exactly what you mean. And and that leads us very nicely on to the second second question, which is those people that have got stuck, they've often tried things themselves, what are the mistakes you see them making? And how do you help them to overcome some of those mistakes?Simone Vincenzi  02:40Yeah, I think that the biggest mistake that they make is that they only realise what they need when it's too late. For example, they work on their offers, and they work on creating a launch. And then they realise that actually, they don't have an audience, or they don't have a community that is ready to buy their offers. And they realise it often after, because I think that when you're running a business, in particular, if it is your first time, or if it is a new industry now is easy to get caught up into doing doing doing and there are millions of things to take care of, there are millions of hat that we are wearing all the time. And it's easy to lose, focus on really what's important, or what's gonna make my launch successful, what's gonna make things work. And I think there are three things that can solve this problem. One is a community. So the focus, having a focus on building your own community and people that they love what you're doing, they respect you, they want to buy from you. They want to listen, they share what you create, and they are your biggest fans, then the second part is lack of visibility and actually becoming really visible to their audience. Because if they don't know that you exist, then how the heck are they going to be part of your community or even buy? And then the third part is your recurring revenues. I think that a lot of people get stressed with that like to get my next client and my next client and my next client and my next client and the debts why they forget to build their community and be visible and is like a self fulfilling prophecy. But if you focus on your recurring revenues, what's happened is that now you have like a good basic income you have a good stable business and then you have more free thing longStuart Webb  04:36yeah, having a stable business or having stable things that you can offer is so much easier and so much sharp. So it's easy to sort of relax into that which you built the audience with. You built the people around you who you know, were there to become the prospective customers don't they?Simone Vincenzi  04:54Exactly. Absolutely. Absolutely. And, and and it is a it is a consistent work is not something that you can do day in and day out, I can do one month in one month off.Stuart Webb  05:06Is it something you see often with, with with people who are starting a business, so they work really hard in order to be able to sort of generate all the ideas. And then they have, then they get a client or they get customers and they get busy doing things for them. And then they forget, they have to go back and start generating new customers. Otherwise, they have a month off, where they're suddenly thinking, I've delivered all those projects, where are all my customers, again, you spend the next month building all those customers back up, don't you that feast and famine is a very common early stage problem.Simone Vincenzi  05:37100%. And I believe that I think that can put things in perspective, in business, often you get paid three to six months down the line for the work that you're doing today. And so the work that you're doing today is what is going to prepare your business for the next three to six months. Not for the revenues. Now, as if you suddenly were today you don't sell for today, but you sell for three to six months down the line? Yes, well, if you do the work today, and day after day, then you can future proof your business in this way by being visible, build your community have a system with the recurring revenues, and then it increase organically. And now you have something that you actually really solid that gives you space that gives you freedom to do other things that or invest in your business or grow it hire more team members and so on.Stuart Webb  06:24Absolutely. Right. Absolutely. Right. So we have scrolling across the screen at the bottom that your website. And I'm hoping that there's some valuable free resources at the website@gtex.org.uk, which community based leaders and the people you're talking to at the moment could go and get those valuable resources from?Simone Vincenzi  06:41Absolutely, there's plenty of free resources out there on their on their website, starting from our podcast, the expert to authority show that everyone can listen to, as well as our we have a training on understanding how to build your community and visibility and recurring revenue. So check it out. And there are and have a look around for your debt training is going to be incredibly valuable.Stuart Webb  07:06Brilliant. So is there a particular concept programme or book which you have found most impactful in your experience? And what is the what's the impact it's had that you'd like to share with us?Simone Vincenzi  07:18Yeah, so I'm Italian, right? No? Yeah, exactly. I'm sure you're being Italian, you know, I'm very family oriented. That's why I'm a big believer in the power of communities. So I'm a big believer in the power of building a circle that is closer than just a following the two of them social media are a number on your mailing list. That is a very tighter network of clients, partners, people that are really there for you and your business, and you're there for them by in return. And there is a great book from Seth Godin, which I absolutely love. And it's called Tribes. Yeah. I don't know if you ever if you read that book, or you heard of it. Yeah.Stuart Webb  08:07Definitely, yeah. Yeah. Very good book. Very, very good book.Simone Vincenzi  08:11Yeah. And it is great, because it talks about how like human beings come together into communities, and our leaders can interact in communities. So then they can stand out from the crowd, while creating something meaningful for society. Incredible book and with big influence in the world that we are doing.Stuart Webb  08:28Yeah, very good. Very good book. Very good book. So Simone, I am sure there is a question that is on your mind at the moment, you're thinking I wish she would ask me this question. So what's the question you would like me to have asked you during this interview? And then answer that question for us. Otherwise, we're going to be spending the next several hours wondering why you asked that question. And I have no answer for it.Simone Vincenzi  08:48Okay, the question is, what are the two main reason the only two reasons why businesses go out of business? They own hands.Stuart Webb  08:56And I would guess that that's it's not for lack of ideas, and often for lack of potential customers, is it?Simone Vincenzi  09:05Mm hmm, exactly. So one is actually they do something illegal. If you do something illegal, you're out of business. The second one is they run out of money, cashStuart Webb  09:16flow. Cash flow, is the key reason that so many businesses run out of steam, isn't it? I've watched it myself many times. And you know, something, Vincent Simona. I often say to people that I'm working with, and they will tell me and this is particularly true of those community based leaders also, but I don't I'm not worried about the money, the money will happen. And I should turn around to them and say, the money is what you have to worry about. You should be thinking about that. Because if you don't have the money, you can't serve your community.Simone Vincenzi  09:48Absolutely. 100 100%. And that's why in particular, if you'd like if you're like a your purpose and your heart is not going to pay the bills. No, I'll let So you make it into a business, which now you means learning about sales and marketing conversions running the community becoming visible. And that's the game. And that's why a lot of people don't succeed a business, because instead of they are too in love, with their purpose and their passion compared to being the love of running the business. And I think that if you do need to combine both to have a successful business, otherwise, there is nothing wrong to do your work for charity. I'm not saying that there is something wrong or that everyone now needs to a business or you're wrong for that. But if you want to have a successful business, you need to fall in love with money, you need to fall in love with running a business and implement your purpose into it. And now you have full personal fulfilment and financial fulfilment together.Stuart Webb  10:44You're absolutely right, Simone. I love that. I mean, yeah, absolutely, you're absolutely spot on with that. I do. So worry about those people that that don't worry about the money because they can't continue to serve the purpose that they have the purpose led life and the purpose led business that they have, if they're not able to pay the bills that will cause the business to fail. So yes, people do you do need to worry about the bills, you do need to worry about whether or not that invoice has been paid. And you need to ring the person and say, that invoice hasn't been paid, I need the money because otherwise I can't keep helping you. That's a really important thing.Simone Vincenzi  11:20And 100%Stuart Webb  11:22Simone, this has been a really brilliant to chat. I really appreciate you spending some time talking to us. That, folks, this is this is why we do these things because we love to get people like Simone who's got really great ideas about how to build really important businesses. And if you want to hear about the people we've got coming on if you were on the on the newsletter list, yesterday, you got an email from me saying join today's call to listen to Simone talk about his business. Get onto our newsletter list by subscribing to it at t ca dot FYI, very simple TCA dot FYI, forward slash subscribe, that's TCA dot FYI, forward slash, subscribe, get onto that list and get on to hearing about some of the wonderful things that people like samode are doing. And if you like some of what we're doing here on the podcast, can you can you rate and subscribe on Apple podcasts and Spotify tell everybody what a great job people like Simone are doing and tell the world that we're trying to help expose more of the sort of great businesses like Simone's doing Simone, thank you so much for coming on and spending a few minutes with us. I really appreciate it. And it's great to hear some of the stuff that you're currently doing with GTExSimone Vincenzi  12:35Thank you very much do I really appreciate and it's been a pleasure being here.Stuart Webb  12:39No problem. Thank you. Get full access to It's Not Rocket Science! at thecompleteapproach.substack.com/subscribe

In this episode, Michael Love joins us to talk about the differential gene expression analysis from bulk RNA-Seq data. We talk about the history of Mike’s own differential expression package, DESeq2, as well as other packages in this space, like edgeR and limma, and the theory they are based upon. Mike also shares his experience of being the author and maintainer of a popular bioninformatics package. Links: Moderated estimation of fold change and dispersion for RNA-seq data with DESeq2 (Love, M.I., Huber, W. & Anders, S.) DESeq2 on Bioconductor Chan Zuckerberg Initiative: Ensuring Reproducible Transcriptomic Analysis with DESeq2 and tximeta And a more comprehensive set of links from Mike himself: limma, the original paper and limma-voom: https://pubmed.ncbi.nlm.nih.gov/16646809/ https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4053721/ edgeR papers: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2796818/ https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3378882/ The recent manuscript mentioned from the Kendziorski lab, which has a Gamma-Poisson hierarchical structure, although it does not in general reduce to the Negative Binomial: https://doi.org/10.1101/2020.10.28.359901 We talk about robust steps for estimating the middle of the dispersion prior distribution, references are Anders and Huber 2010 (DESeq), Eling et al 2018 (one of the BASiCS papers), and Phipson et al 2016: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3218662/ https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6167088/ https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5373812/ The Stan software: https://mc-stan.org/ We talk about using publicly available data as a prior, references I mention are the McCall et al paper using publicly available data to ask if a gene is expressed, and a new manuscript from my lab that compares splicing in a sample to GTEx as a reference panel: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3013751/ https://doi.org/10.1101/856401 Regarding estimating the width of the dispersion prior, references are the Robinson and Smyth 2007 paper, McCarthy et al 2012 (edgeR), and Wu et al 2013 (DSS): https://pubmed.ncbi.nlm.nih.gov/17881408/ https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3378882/ https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3590927/ Schurch et al 2016, a RNA-seq dataset with many replicates, helpful for benchmarking: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4878611/ Stephens paper on the false sign rate (ash): https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5379932/ Heavy-tailed distributions for effect sizes, Zhu et al 2018: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6581436/ I credit Kevin Blighe and Alexander Toenges, who help to answer lots of DESeq2 questions on the support site: https://www.biostars.org/u/41557/ https://www.biostars.org/u/25721/ The EOSS award, which has funded vizWithSCE by Kwame Forbes, and nullranges by Wancen Mu and Eric Davis: https://chanzuckerberg.com/eoss/proposals/ensuring-reproducible-transcriptomic-analysis-with-deseq2-and-tximeta/ https://kwameforbes.github.io/vizWithSCE/ https://nullranges.github.io/nullranges/ One of the recent papers from my lab, MRLocus for eQTL and GWAS integration: https://mikelove.github.io/mrlocus/

INTRO Karen Bashford AKA The Manifesting Lady Karen has experienced 2 abusive and traumatic relationships, which has lead her to discovering why some women, are more prone to abuse and trauma, than others.  She now uses her knowledge and skills to educate women, how the past affects their present reality.  As a change mentor and energy healer, she releases them from the shackles of their past to powerfully manifest and influences their future, to be happy and fulfilled in all areas of their lives.   CONTENT Domestic abuse is not only physical, it can be psychological, emotional, sexual, financial or verbal. Some of these behaviours can be so subtle that we can be fooled into believing we are to blame. As a result of the abuse, Karen developed an auto-immune disease. Finally, she realised that she had to leave the relationship, and began to heal from the experience. For the past 10 years, Karen has been helping women to lead happy and fulfilled lives.   BIO Karen Bashford, as a survivor of two abusive and traumatic relationships, after what appeared to be a normal happy childhood, empowers women to become influential leaders, by releasing them from the shackles of the past, which have held them stuck. In gaining more confidence, time, money, and peace of mind, they demonstrate how powerful they are, creating a ripple effect at home, and within their work environment which expands into the world in general. For the last 10 years, Karen has combined her psychic abilities as an Energy Healer, and Tarot Reader together with her coaching skills to rapidly and effectively change the lives of hundreds of women. Karen knows how unconscious bias and limiting beliefs along with the ancestral, generational, and present energy of every relationship, can affect the ability to create a happy, healthy, wealthy life in loving relationships. Starting her professional career in banking choosing redundancy after 20 years, she became a Financial Adviser.  But upon divorcing her husband Karen changed direction to work as a Personal Assistant, later setting up a successful secretarial business.  However, her holistic and psychic skills, lead Karen to train as a Hypnotherapist and “NLP” Neuro-Linguistic Program Facilitator. Karen uses a three-pronged approach in her work to empower women… Education – In the past and present women have been controlled and manipulated by cultural and social programming, to the point where they actively participate, because they don’t know how to be the Change – There is no better time than now to throw off the shackles of the past to actively make choices which influences how women are accepted and received in the world. With freedom from the past women are capable of powerfully Manifesting, a lifestyle which serves all equally, through demonstrating unity and harmony, rather than separation and conflict.  Karen speaks on: The Power of Manifesting With Purpose Why women can be leaders How abuse and trauma is repeated unconsciously Why ME? Karen’s story of discovering the power of forgiveness and surrendering to the Universe How trauma affects productivity and income Becoming you… Taking back control of your thoughts and feelings.   As a speaker: Le Parente Celebrate Love 2020 Aloud Conversations The RAIS Monthly speaker event GTeX’s Purpose to Abundance events The PowerHouse Conferences.   Participated in the following podcasts: MumsSoloProject, PrincessPower, The Enlightened Entrepreneur, Jonny Hates Marketing, Business School for Therapists, Coaches and Healers.  Karen has presented at and run numerous networking events over 5 years. She has also been interviewed on Croydon radio and Brooklands radio. Karen’s book - How to Be Imperfectly Perfect – A Practical Spiritual Guide to Becoming YOU publishes in January 2021. She has co-authored The Journey Beyond - inspirational stories of success and Generation W - celebrating 100 years of women’s vote. CONTACT DETAILS / LINKS Website - http://www.karenbashford.com Facebook - www.facebook.com/KarenABashford Facebook - www.facebook.com/TheManifestingLady/ Linkedin -www.linkedin.com/in/karenbashford/ Instagram -https://www.instagram.com/karenbashford4243/ Twitter -https://mobile.twitter.com/karenb101 Email -   karen@karenbashford.com Mobile - 07818 096673   ABOUT WENDY CAPEWELL Wendy is a Psychotherapist, Coach, Public Speaker, and Author. She helps people who are stuck either in their personal, professional or relationships, get to the root of the problem which is holding them back, let go - and move forward into a happier more successful life.   Connect with Wendy Here...... Facebook - https://www.facebook.com/Copewelltherapies Linked in - https://www.linkedin.com/in/wendycapewell/ Website - https://www.wendycapewell.co.uk/ Book –‘From Surviving to Thriving in a Romantic Relationship’- Link to Amazon Email – info@wendycapewell.co.uk Email me if you would like to receive my newsletter. which contains my latest news, articles and special offers. If you have enjoyed this show then please leave a review.      

In this episode Simone reveals his journey from starting out in a barn on a farm to the world stage. Simone Vincenzi turns experts into authorities so they can grow their business faster, attract clients with ease and change the world. Simone say: I believe everyone has the right to create a life of fulfilment, no matter their background. I am passionate about making things simpler for entrepreneurs, experts and dreamers that have been working hard to create any success in their lives, so they can keep having a sense of purpose and meaning in their work.

Welcome to another episode of Explode Your Expert Biz Show, brought to you by http://gtex.org.uk/, I am your host, Simone Vincenzi, The Experts Strategist, and this is the podcast for experts who want to become the ultimate authority in their niche while making an impact in the world. In this episode, I talk about GTeX review of the year and talking about our plans for 2021. A live video podcast recording with not much sense other than me talking about stuff in my mind. Connect with Simone Linkedin https://www.linkedin.com/in/simone-vincenzi/ Facebook https://www.facebook.com/simonegtex To become a GTeX Member, Apply here: https://gtex.events/call ----- To receive daily support in your coaching and speaking business, join our private Facebook Group EXPLODE YOUR EXPERT BIZ https://www.facebook.com/groups/explodeyourexpertbiz/ ------- Take a full business assessment for free to have absolute clarity on your business with the EXPERT BIZ CHECKLIST. http://bit.ly/expert-biz-checklist-podcast ------ We have created the Ultimate Publicity Bundle where you will get access to: A Curated Database Of 500+ Media Publications That Are Looking For Speakers 100+ Podcasts looking for guests. My templates on how to connect with journalists. My follow up framework to maximise every single speaking opportunity you have. All of this for only £29,99 for a limited period of time. Click here to download. http://bit.ly/publicity-bundle-podcast Also, make sure you subscribe to the podcast so you don’t miss any other episode. If you want to reach out to me with your questions, you can email me at Simone@gtex.org.uk that comes right to my inbox. --- Send in a voice message: https://anchor.fm/explode/message

Welcome to another episode of Explode Your Expert Biz Show, brought to you by http://gtex.org.uk/, I am your host, Simone Vincenzi, The Experts Strategist, and this is the podcast for experts who want to become the ultimate authority in their niche while making an impact in the world. We have created the Ultimate Publicity Bundle where you will get access to: A Curated Database Of 500+ Media Publications That Are Looking For Speakers 100+ Podcasts looking for guests. My templates on how to connect with journalists. My follow up framework to maximise every single speaking opportunity you have. All of this for only £29,99 for a limited period of time. Click here to download. http://bit.ly/publicity-bundle-podcast Today I have the pleasure to Interview Caroline Micallef Wragg We incorporate Strategic Marketing, Communications and Sales for SME’s, business owners and larger corporations that want to excel in their areas of expertise and more. As your strategic partner to you, your health and your business, we have created unique systems and solutions that give you the Leading Edge in your industry and business, raising your profile, as well as connecting you with key influencers and clients that see you and want to buy your products and services. In this episode, we talk about: How Caroline Micallef Wragg created and filled her group program using GTeX method Connect with Caroline Micallef Wragg Website https://www.starflowercompany.co.uk/ Linkedin: https://www.linkedin.com/in/caroline-micallefwragg/ To become a GTeX Member, Apply here: https://gtex.events/call To receive daily support in your coaching and speaking business, join our private Facebook Group EXPLODE YOUR EXPERT BIZ https://www.facebook.com/groups/explodeyourexpertbiz/ Take a full business assessment for free to have absolute clarity on your business with the EXPERT BIZ CHECKLIST. http://bit.ly/expert-biz-checklist-podcast Also, make sure you subscribe to the podcast so you don’t miss any other episode. If you want to reach out to me with your questions, you can email me at Simone@gtex.org.uk that comes right to my inbox. --- Send in a voice message: https://anchor.fm/explode/message

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2020.11.10.376228v1?rss=1 Authors: Bartha, A., Gyorffy, B. Abstract: Genes showing higher expression in either tumor or metastatic tissues can help in better understanding tumor formation, and can serve as biomarkers of progression or as therapy targets with minimal off-target effects. Our goal was to establish an integrated database using available transcriptome-level datasets and to create a web-platform enabling mining of this database by comparing normal, tumor and metastatic data across all genes in real time. We utilized data generated by either gene arrays or RNA-seq. Gene array data were manually selected from NCBI-GEO. RNA sequencing data was downloaded from the TCGA, TARGET, and GTEx repositories. TCGA and TARGET contain predominantly tumor and metastatic samples from adult and pediatric patients, while GTEx samples are from healthy tissues. Statistical significance was computed using Mann-Whitney or Kruskall-Wallis tests. The entire database contains 56,938 samples including 33,520 samples from 3,180 gene chip-based studies (453 metastatic, 29,376 tumorous and 3,691 normal samples), 11,010 samples from TCGA (394 metastatic, 9,886 tumorous and 730 normal), 1,193 samples from TARGET (1 metastatic, 1,180 tumor, 12 normal) and 11,215 normal samples from GTEx. The most consistently up-regulated genes across multiple tumor types were TOP2A (mean FC=7.8), SPP1 (FC=7.0) and CENPA (FC=6.03) and the most consistently down-regulated gene was ADH1B (mean FC=0.15). Validation of differential expression using equally sized training and test sets confirmed reliability of the database in breast, colon, and lung cancer (p

On this episode, we sat with Simone Vincenzi who started his entrepreneurial journey working in a restaurant at a young age. However, he invested his time in reading books with like minded authors. He is now a speaker who turns experts to authorities. One of the highlights from this episode is think about building your community as a business owner or an entrepreneur and in the long run your community will take care of your business.  --- Send in a voice message: https://anchor.fm/askdpros-business-podcast/message

My guest this week, Simone Vincenzi, Founder/CEO of GTeX, a London-based company, and I discuss how becoming an authority in your field starts with these four pillars. Get clarity on your business model and turn chaos into simplicity and flow. Create HIGH-CONVERTING offers that deliver a real transformation. Expand your audience and reach to get in front of your ideal clients consistently. Grow your influence to get access to unlimited opportunities and attract clients with ease. To learn more, visit: https://gtex.org.uk/

My guest this week, Simone Vincenzi, Founder/CEO of GTeX, a London-based company, and I discuss how becoming an authority in your field starts with these four pillars.Get clarity on your business model and turn chaos into simplicity and flow.Create HIGH-CONVERTING offers that deliver a real transformation.Expand your audience and reach to get in front of your ideal clients consistently.Grow your influence to get access to unlimited opportunities and attract clients with ease.To learn more, visit: https://gtex.org.uk/

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2020.10.26.355941v1?rss=1 Authors: Yang, G., Sabunciyan, S., Florea, L. D. Abstract: Alternative splicing of mRNA is an essential gene regulatory mechanism with important roles in development and disease. We present MntJULiP, a method for comprehensive and accurate quantification of splicing differences between two or more conditions. MntJULiP implements novel Dirichlet-multinomial and zero-inflated negative binomial models within a Bayesian framework to detect both changes in splicing ratios and in absolute splicing levels of introns with high accuracy, and can find classes of variation overlooked by reference tools. Additionally, a mixture model allows multiple conditions to be compared simultaneously. Highly scalable, it processed hundreds of GTEx samples in

Simone Vincenzi, known as The Experts Strategist, is the co-founder of GTeX, Forbes and Huffington Post contributor, TEDx speaker, host of the podcast Explode Your Expert Business, author of 3 life-changing books. He is passionate about turning experts into authorities using his signature EXPLODE YOUR EXPERT BIZ programs. Every year he speaks in front of more than 5,000 business leaders, which included sharing the stage with Les Brown, Dr John DeMartini, Trent Shelton and many more. He has also organized over 170 events, including Gary Vee speaking from York Hall boxing ring. Following his passion for making an impact on the younger generation, he also partners with the largest youth organizations in the UK, helping them deliver more effective courses and training. To register for Simone and Michelle's free training and to learn the top 4 strategies to get known in your niche, happening Thursday October 29th, 2020 at 1 pm EST, go to https://www.naturalborncoaches.com/attraction.

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2020.10.01.322164v1?rss=1 Authors: Karasikov, M., Mustafa, H., Danciu, D., Zimmermann, M., Barber, C., Ratsch, G., Kahles, A. Abstract: The amount of biological sequencing data available in public repositories is growing exponentially, forming an invaluable biomedical research resource. Yet, making all this sequencing data searchable and easily accessible to life science and data science researchers is an unsolved problem. We present MetaGraph, a versatile framework for the scalable analysis of extensive sequence repositories. MetaGraph efficiently indexes vast collections of sequences to enable fast search and comprehensive analysis. A wide range of underlying data structures offer different practically relevant trade-offs between the space taken by an index and its query performance. Achieving compression ratios of up to 1,000-fold over the already compressed raw input data, MetaGraph indexes can represent the content of large sequencing archives in the working memory of a single compute server. We demonstrate our framework's scalability by indexing over 1.4 million whole genome sequencing (WGS) records from NCBI's Sequence Read Archive, representing a total input of more than three petabases. MetaGraph provides a flexible methodological framework allowing for index construction to be scaled from consumer laptops to distribution onto a cloud compute cluster for processing terabases to petabases of input data. Notably, processing of data sets ranging from 1 TB of raw WGS reads to 20 TB of human RNA-sequencing data results in indexes whose memory footprints are small enough to host on standard desktop workstations. Besides demonstrating the utility of MetaGraph indexes on key applications, such as experiment discovery, sequence alignment, error correction, and differential assembly, we make a wide range of indexes available as a community resource, including indexes of over 450,000 microbial WGS records, more than 110,000 fungi WGS records, and more than 40,000 whole metagenome sequencing records. A subset of these indexes is made available online for interactive queries. All indexes will be available for download and in the cloud. In total, indexes comprising more than 1 million sequencing records are available for download. As an example of our indexes' integrative analysis capabilities, we introduce the concept of differential assembly, which allows for the extraction of sequences present in a foreground set of samples but absent in a given background set. We apply this technique to differentially assemble contigs to identify pathogenic agents transfected via human kidney transplants. In a second example, we indexed more than 20,000 human RNA-Seq records from the TCGA and GTEx cohorts and use them to extract transcriptome features that are hard to characterize using a classical linear reference. We discovered over 200 trans-splicing events in GTEx and found broad evidence for tissue-specific non-A-to-I RNA-editing in GTEx and TCGA. Copy rights belong to original authors. Visit the link for more info

Welcome to another episode of Explode Your Expert Biz Show, brought to you by http://gtex.org.uk/, I am your host, Simone Vincenzi, The Experts Strategist, and this is the podcast for experts who want to become the ultimate authority in their niche while making an impact in the world. To join our Explode Your Expert Biz Intensive - Click here: https://simonev.co/launch Today I have the pleasure to Interview Siawash Zahmat He’s a former professional poker player and earned a renowned reputation and success within the scene. Having gone through a life-changing experience, he was inspired to re-evaluate his journey and stepped away in order to pursue a more fulfilling career. This is when Siawash immersed himself even more into self-development and chose to be a force in changing people’s lives, which subsequently led him to become a coach. Noticing the lack of leadership in the Muslim Coaching scene as well as a community network, self development and not enough businesses with integrity, he teamed up with his business partner Asiya Othman and decided to start the first Muslim Coaching Academy and subsequently an exclusive Muslim Mastermind where like minded entrepreneurs, coaches get together to create shifts leading them to more success and aid them in scaling their businesses. In this episode, we talk about: How Siawash turned Pro What he learnt from the experience working with GTeX that helped his business grow The #1 Piece of advice for any coach wanting to grow a business Connect with Siawash Zahmat Website https://themuslimcoachingacademy.com/ Linkedin https://www.linkedin.com/in/siawash-zahmat-6a07a9a9/ Facebook https://www.facebook.com/siwash.zahmat To become a GTeX Member, Apply here: https://gtex.events/call ----- To receive daily support in your coaching and speaking business, join our private Facebook Group EXPLODE YOUR EXPERT BIZ https://www.facebook.com/groups/explodeyourexpertbiz/ ------- Take a full business assessment for free to have absolute clarity on your business with the EXPERT BIZ CHECKLIST. http://bit.ly/expert-biz-checklist-podcast --- Send in a voice message: https://anchor.fm/explode/message

First up this week, Senior Correspondent Daniel Clery talks with host Sarah Crespi about how Breakthrough Listen—a privately funded initiative that aims to spend $100 million over 10 years to find extraterrestrial intelligent life—has changed the hunt for alien intelligence-link.  And as part of a special issue on the Genotype-Tissue Expression  (GTEx) Project, Brandon Pierce, a professor in the Departments of Public Health Sciences and Human Genetics at the University of Chicago, joins Sarah to discuss his group's work on variation in the protective caps at the end of our chromosomes. The gradual shortening of these caps, also known as telomeres, has been associated with aging.  Read more from the GTEx special issue. This week's episode was produced with help from Podigy. Listen to previous podcasts About the Science Podcast Download a transcript (PDF) [Image: V. Altounian/Science; Music: Jeffrey Cook] Authors: Sarah Crespi; Dan Clery See omnystudio.com/listener for privacy information.

First up this week, Senior Correspondent Daniel Clery talks with host Sarah Crespi about how Breakthrough Listen—a privately funded initiative that aims to spend $100 million over 10 years to find extraterrestrial intelligent life—has changed the hunt for alien intelligence-link.  And as part of a special issue on the Genotype-Tissue Expression  (GTEx) Project, Brandon Pierce, a professor in the Departments of Public Health Sciences and Human Genetics at the University of Chicago, joins Sarah to discuss his group's work on variation in the protective caps at the end of our chromosomes. The gradual shortening of these caps, also known as telomeres, has been associated with aging.  Read more from the GTEx special issue. This week's episode was produced with help from Podigy. Listen to previous podcasts About the Science Podcast Download a transcript (PDF) [Image: V. Altounian/Science; Music: Jeffrey Cook] Authors: Sarah Crespi; Dan Clery See omnystudio.com/listener for privacy information.

First up this week, Senior Correspondent Daniel Clery talks with host Sarah Crespi about how Breakthrough Listen—a privately funded initiative that aims to spend $100 million over 10 years to find extraterrestrial intelligent life—has changed the hunt for alien intelligence.  And as part of a special issue on the Genotype-Tissue Expression (GTEx) project, Brandon Pierce, a professor in the Departments of Public Health Sciences and Human Genetics at the University of Chicago, joins Sarah to discuss his group’s work on variation in the protective caps at the end of our chromosomes. The gradual shortening of these caps, also known as telomeres, has been associated with aging. Read more from the GTEx special issue. This week’s episode was produced with help from Podigy. Listen to previous podcasts. About the Science Podcast Download a transcript (PDF). [Image: V. Altounian/Science; Music: Jeffrey Cook]

There are three things that Simone cannot live without; one is speaking, one is basketball and one is playing the digeridoo. These days a successful entrepreneur, podcaster, three- time author and motivational speaker, his business - GTEX - turns experts into authorities by providing a framework business model to enable passionate entrepreneurs and solo-preneurs to get their voices heard and make income as well as impact. It was not always thus. “I am a very driven person. I am all in or nothing.” Simone explains the pivotal moments in his life and how they shaped him and still do. After his parents split at 14, Simone was determined to take responsibility for himself and help provide for his mother. An all-in detour via hospitality saw him go from waiter to managing a Michelin starred restaurant aged 19. A self-initiated stint living out of a backpack and effectively being homeless for two years led to an activist phase, helping to set up Occupy London, outside the London Stock Exchange. Today he is paid up part of the capitalism that he used to rebel against. “It’s an interesting paradox that there I was shouting “screw capitalism!” and now here I am running three businesses” he laughs. Whilst creating change and making an impact has always been a core theme, Simone has changed his approach; “I was living in a world of conspiracy theories. Activism is about destroying and I realised I prefer to impact through creating something positive, something new.” Simone's passion is infectious as he invites us all focus on the positive and explains how what you focus on expands. If you’re an entrepreneur looking to explode your business, then this podcast is for you! Smash The Box is a personal development business. Everything I do is with the aim of inspiring you to find your purpose so you can make your mark on the world. One way I do this is by sharing people’s stories. Inspiring stories that the world needs to hear. Stories of breakthroughs, of adversity overcome, of achievements, successes, of setbacks and turnarounds, of realisations and lightbulb moments. This audio experience is a natural and perfect complement to the existing services of Life Coaching, Leadership Coaching, Breakthrough Workshops and Inspirational Talks - all of which forms something quite unique and special. If you are looking to find your purpose in life, in work, or both, then don’t just think outside the box. It’s time to Smash The Box! Connect with us! www.smashthebox.me www.youtube.com/channel/UC7fEEvjX52qS928oyLGCtuQ www.facebook.com/SmashTheBoxMarkPitcher/ www.instagram.com/markpitcher_smashthebox/

Welcome to another episode of Explode Your Expert Biz Show, brought to you by http://gtex.org.uk/, I am your host, Simone Vincenzi, The Experts Strategist, and this is the podcast for experts who want to become the ultimate authority in their niche while making an impact in the world. To become a GTeX Member, Apply here: https://gtex.events/call The more you are known, the faster you will grow your business. One of the best ways to get known in your field and build your profile is to get featured on media and publications. But finding the right publications can take ages. Also, how do you connect with journalists and podcasters in the right way? How do you follow up to get the most of every feature you have? This is why my team and I have spent an entire month and more than £2000 consolidating 4 years of data on the global publications that are looking for contributors. We have created the Ultimate Publicity Bundle where you will get access to: A Curated Database Of 500+ Media Publications That Are Looking For Speakers 100+ Podcasts looking for guests. My templates on how to connect with journalists. My follow up framework to maximise every single speaking opportunity you have. All of this for only £29,99 for a limited period of time. Click here to download. http://bit.ly/publicity-bundle-podcast In this episode, I talk about: Win more than $2000 worth of prizes, business mentoring and training I wanted to do something special for episode #300 of Explode Your Expert Biz Show https://podcasts.apple.com/gb/podcast/explode-your-expert-biz-show/id1065552208 https://anchor.fm/explode In this episode I share my top 5 learning from turning our event business into a successful online business during Covid. I will also talk about our business model and show you the behind the scene of how GTeX works. It’s going to be an epic show. By leaving a review on Apple Podcast you will also have a chance to win: #1 Profile Builder Online Program $1000 #2 Offer Launch Accelerator $1000 #3 Publicity Bundle $200 Leave a review and then send me a screenshot with the review to win. Thanks for supporting the show for the past 3 years. You are awesome. To become a GTeX Member, Apply here: https://gtex.events/call ----- To receive daily support in your coaching and speaking business, join our private Facebook Group EXPLODE YOUR EXPERT BIZ https://www.facebook.com/groups/explodeyourexpertbiz/ ------- Take a full business assessment for free to have absolute clarity on your business with the EXPERT BIZ CHECKLIST. http://bit.ly/expert-biz-checklist-podcast --- Send in a voice message: https://anchor.fm/explode/message

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2020.07.23.206391v1?rss=1 Authors: Al Bkhetan, Z., Chana, G., Soon Ong, C., Goudey, B., Kotagiri, R. Abstract: Motivation: The high accuracy of current haplotype phasing tools has enabled the interrogation of haplotype (or phase) information more widely in genetic investigations. Including such information in eQTL analysis complements SNP-based approaches as it has the potential to detect associations that may otherwise be missed. Results: We have developed a haplotype-based eQTL approach called eQTLHap to investigate associations between gene expression and haplotype blocks. Using simulations, we demonstrate that eQTLHap significantly outperforms typical SNP-based eQTL methods when the causal genetic architecture involves multiple SNPs. We show that phasing errors slightly impact the sensitivity of the proposed method (< 4%). Finally, the application of eQTLHap to real GEUVADIS and GTEx datasets finds 22 associations that replicated in larger studies or other tissues and could not be detected using a single-SNP approach. Availability: https://github.com/ziadbkh/eQTLHap Copy rights belong to original authors. Visit the link for more info

From his humble beginnings as a Chef to a Speaker & Experts Strategist, Simone Vincenzi, the co-founder of GTeX has come a long way. Passionate about turning experts into authorities using his signature EXPLODE YOUR EXPERT BIZ programs, Simone has helped several thousand business leaders and shared the stage with the likes of Les Brown, Dr. John DeMartini, Trent Shelton, and others. In this episode, we discuss what it takes to become an authority in your chosen field and make your biggest impact yet!

Welcome to another episode of Explode Your Expert Biz Show, brought to you by http://gtex.org.uk/, I am your host, Simone Vincenzi, The Experts Strategist, and this is the podcast for experts who want to become the ultimate authority in their niche while making an impact in the world. To become a GTeX Member, Apply here: https://gtex.events/call Today I have the pleasure to Interview Sarah Poynton-Ryan An evolving, curious and ambitious property nerd. Known for her crazy side projects, planning trips abroad, serious Starbucks addiction and creative thinking. Sarah uses her own personal journey as a property entrepreneur to teach and inspire others. In this episode, we talk about: How she sold £64k from a 5-day challenge The behind the scene of the challenge what she did to make it work Connect with Sarah Poynton-Ryan Facebook: https://www.facebook.com/profile.php?id=508628084 To become a GTeX Member, Apply here: https://gtex.events/call ----- To receive daily support in your coaching and speaking business, join our private Facebook Group EXPLODE YOUR EXPERT BIZ https://www.facebook.com/groups/explodeyourexpertbiz/ ------- Take a full business assessment for free to have absolute clarity on your business with the EXPERT BIZ CHECKLIST. http://bit.ly/expert-biz-checklist-podcast ------ We have created the Ultimate Publicity Bundle where you will get access to: A Curated Database Of 500+ Media Publications That Are Looking For Speakers 100+ Podcasts looking for guests. My templates on how to connect with journalists. My follow up framework to maximise every single speaking opportunity you have. All of this for only £29,99 for a limited period of time. Click here to download. http://bit.ly/publicity-bundle-podcast Also, make sure you subscribe to the podcast so you don’t miss any other episode. If you want to reach out to me with your questions, you can email me at Simone@gtex.org.uk that comes right to my inbox. --- Send in a voice message: https://anchor.fm/explode/message

Adam Cox is joined by Simone Vincenzi, founder and owner of GTEx: a personal development training company. Simone is one of the UK’s leading speakers on the topic of authority and offers insight into how people and brands can increase their authority with their ideal customer or audience. Simone explains why things such as public speaking, media coverage, and other approaches can hugely impact a person’s perceived authority, leading to higher earning power and brand positioning in any market.

#106 - One Money Making Strategy We Are Implementing Now In GTeX. #pijamatopnotjustbottom Simone Vincenzi Talks Business - Season 3 Coronavirus Special In this episode I talk about: - A very simple and effective strategy to make money now in your business. - How to find money you did not even know you had. - Decisions we have made during our finance meeting today with Ben Shorter. * Ps. To catch our daily episode subscribe to Simone Vincenzi Talks Business on your favourite podcasting platform. Thanks for joining! To subscribe to the podcast and listen to all the episodes click here https://anchor.fm/simone-vincenzi

Simone Vincenzi Talks Business - Season 3 Coronavirus Special #103 - Sales pages, selling to corporate and dealing with finances. In this episode I talk about: - The one tweak that boosted our sales pages conversions. - How Ben Shorter and Liz Walsh are getting new corporate clients. - What Ben and I are doing at GTeX to keep cashflow positive. To catch our daily episode subscribe to Simone Vincenzi Talks Business on your favourite podcasting platform. https://anchor.fm/simone-vincenzi

Welcome to another episode of Explode Your Expert Biz Show, brought to you by http://gtex.org.uk/,    I am your host, Simone Vincenzi, The Experts Strategist, and this is the podcast for experts who want to become the ultimate authority in their niche while making an impact in the world.   The more you are known, the faster you will grow your business. One of the best ways to get known in your field and get clients is speaking at events.   But finding the right events can take ages. Also how do you connect with event organisers in the right way? How do you follow up to get the most of every speaking gig you have?   This is why my team and I have spent an entire month and more than £2000 consolidating 4 years of data of the best conferences and events in Uk and Europe that are looking for speakers.   We have created the Ultimate Speaking Bundle where you will get access to:   A Curated Database Of 100+ Events And Conferences In The UK And Europe That Are Looking For Speakers My templates on how to connect with event organisers My follow up framework to maximise every single speaking opportunity you have.   All of this for only £29,99 for a limited period of time.   Click here to download. http://bit.ly/eventdatabasepodcast   In this episode, I interview the GTeX member of the month Michelle Raymond   Michelle Raymond is a HR Consultant and Business Growth Specialist who helps professionals around the world gain absolute CLARITY, upgrade their CONFIDENCE, create CONSISTENCY and CONTINUAL growth within their business.   Michelle is a certified Master practitioner in NLP, an international mentor and public speaker, multi award winning entrepreneur and GDPR expert.   Michelle is also in charge of our Profile Builder Program, supporting our members to get known, get paid and make an impact. To become a GTeX Member, Apply here: https://gtex.events/call    -----   To receive daily support in your coaching and speaking business, join our private Facebook Group EXPLODE YOUR EXPERT BIZ https://www.facebook.com/groups/explodeyourexpertbiz/   -------   Take a full business assessment for free to have absolute clarity on your business with the EXPERT BIZ CHECKLIST. http://bit.ly/expert-biz-checklist-podcast ------

In this episode and have a chat with the very lovely Simone Vincenzi from GTex. Simone is an International Speaker, a Coach and The Experts Strategist. He has contributed to Forbes and Entrepreneur magazines. We discuss mindset, emotional regulation how he helps people become known in their field and much more

Welcome to another episode of Explode Your Expert Biz Show, brought to you by http://gtex.org.uk/,    I am your host, Simone Vincenzi, The Experts Strategist, and this is the podcast for experts who want to become the ultimate authority in their niche while making an impact in the world.   If you want to build a highly profitable coaching, speaking or training business, download now our EXPERT BIZ CHECKLIST.   The EXPERT BIZ CHECKLIST is the best tool you can use for your expert business where you will take a full assessment of your business and know exactly what to focus on to go to your next level.   Click here to download. http://bit.ly/expert-biz-checklist-podcast Today I have the pleasure to Interview Dilys Sillah   Dilys Sillah is a Transformational Life Coach, specialising in emotional independence and relationship coaching. She is a TEDx speaker and founder of former charity 'Who Will Hear My Cry' (WWHMC) that raised awareness on rape, child abuse and domestic violence. In this episode, we talk about:  Best selling book Not This Widow in 6 months. Her story that will leave you speechless. Connect with Dilys Sillah   Website:  https://dilyssillah.com/   Social: https://www.facebook.com/dilys.sillah https://twitter.com/DilysSillah https://www.instagram.com/dilyssillah/ Book: https://www.amazon.co.uk/Not-This-Widow-Strength-Survival-ebook/dp/B083D7GKKT?fbclid=IwAR0n7elD2J8IiQAulpBHKt9g1l30JcvErvQe6m0dPpDqPAPCBvhSUyqp2Gk To become a GTeX Member, Apply here: https://gtex.events/call     To receive daily support in your coaching and speaking business, join our private Facebook Group EXPLODE YOUR EXPERT BIZ https://www.facebook.com/groups/explodeyourexpertbiz/   Take a full business assessment for free to have absolute clarity on your business with the EXPERT BIZ CHECKLIST. http://bit.ly/expert-biz-checklist-podcast

Welcome to another episode of Explode Your Expert Biz Show, brought to you by http://gtex.org.uk/,    This is the podcast for experts who want to become the ultimate authority in their niche while making an impact in the world. Episode 257 is a special episode   If you want to build a highly profitable coaching, speaking or training business, download now our EXPERT BIZ CHECKLIST.   The EXPERT BIZ CHECKLIST is the best tool you can use for your expert business where you will take a full assessment of your business and know exactly what to focus on to go to your next level.   Click here to download. http://bit.ly/expert-biz-checklist-podcast Today is all about Purpose To Abundance. This is the event that we are going to do together on the 18th of November How to create peace and freedom in your life with Karen Bashford & Susan Wiechert    Safoora Ahmed, GTeX business coach is interviewing Karen and Susan.   As a creative and inspiring change-maker, Susanne Wiechert will ignite your inner-health champion and inspire you in unconventional ways. With a love for variety in life, she offers a unique combination of services (including energy medicine, mindfulness, neuroscience and nutrition) that integrate creative flair with professional magnanimity.   Connect with Susan Wiechert https://www.facebook.com/susanne.wiechert https://www.facebook.com/energizemeUK/ Karen Bashford, known as The Manifesting Lady, a Transformational Coach, Energy Healer and Tarot Reader has been guiding and coaching clients for over 10 years to rapidly and effectively transform their lives.   Connect with Karen Bashford https://www.facebook.com/KarenABashford To become a GTeX Member, Apply here: https://gtex.events/call   To receive daily support in your coaching and speaking business, join our private Facebook Group EXPLODE YOUR EXPERT BIZ https://www.facebook.com/groups/explodeyourexpertbiz/   Take a full business assessment for free to have absolute clarity on your business with the EXPERT BIZ CHECKLIST. http://bit.ly/expert-biz-checklist-podcast Also, make sure you subscribe to the podcast so you don’t miss any other episode.   If you want to reach out to me with your questions, you can email me at Simone@gtex.org.uk which comes right to my inbox.   --- Send in a voice message: https://anchor.fm/explode/message

Welcome to episode #253 of Explode Your Expert Biz Show, brought to you by http://gtex.org.uk/,    If you want to make 6 figures presentations and become awesome at Selling From The Stage without compromising your integrity I have created The Ultimate Selling From Stage Checklist.    The most comprehensive checklist to create a pitch that sells without being a douchebag. http://bit.ly/Selling-Checklist-Podcast In this episode, I talk about   The secret to becoming a thought leader in your industry and attract high-paying clients. What I am going to share with you in this training generated more than $1M for our clients. It's my absolute favourite strategy and the one we use the most in GTeX too. This is an absolute must for you as an expert if you want to establish your authority in your field and attract high-paying clients. To become a GTeX Member, Apply here: https://gtex.events/call   To receive daily support in your coaching and speaking business, join our private Facebook Group EXPLODE YOUR EXPERT BIZ https://www.facebook.com/groups/explodeyourexpertbiz/ Also make sure you subscribe to the podcast so you don’t miss any other episode.   If you want to reach out to me with your questions, you can email me at Simone@gtex.org.uk that comes right to my inbox. --- Send in a voice message: https://anchor.fm/explode/message

How To Build Yourself And Maintain It Simone Vincenzi, known as The Experts Strategies, co-founder of GTeX, Forbes and Huffington Post contributor, TEDx speaker and host of the podcast. He speaks in front of thousands of business leaders and organized over hundreds of event, including Gary Vee speaking from York Hall. Lastly, he also partners with the largest youth organization in the UK. Simone is a great friend who I have watched from strength to strength. He is extremely passionate helping people. In this episode we discuss the growth of gtex his company, His journey from being totally broke to his current success.

Todays guest joining us on the show, is not just a second repeater as he first appeared on the show back on episode 574 of the show. But also I firmly class him as a friend, who has entered the inner circle of people that i trust, respect and consider to be doing things the right way.He is known as the Experts Strategist, the co-founder of GTeX, also he is a Forbes and Huffington Post contributor, TEDx speaker, host of the podcast Explode Your Expert Business, author of 3 life-changing books. He is passionate about turning experts into authorities using his signature EXPLODE YOUR EXPERT BIZ programs. Every year he speaks in front of more than 5,000 business leaders, which included sharing the stage with Les Brown, Dr John DeMartini, Trent Shelton and many more.He has also organized over 170 events, including Gary Vee speaking from York Hall boxing ring. Following his passion for making an impact on the younger generation, he also partners with the largest youth organizations in the UK, helping them deliver more effective courses and training.He is a man who has a desire to see the world flourish, and help individuals across the globe to grow exponentially, becoming their greatest versions.But of course to get to that point, you truly have to go through a journey of discovery yourself, to find your inner skills that make you unique and valuable to everyone.And it would be fair to say that just a few years ago, our guest was many many steps away from discovering his purpose in life.He was working in a restaurant in Notting Hill as a waiter, as he had been doing since the age of 15, and had certain mental restrictions should we say, that were holding him back from creating his dream life.He didn't have a degree and his level of English was not quite enough to understand the self development literature that he was starting to immerse himself in.As he says, “Just a few years ago I was facing a very difficult moment of my life. I knew I had to change but I didn't know what to do. I felt lost, like wondering in a circle in a dark room with no exit sign and this wasn't me!! I have always been the uber-positive-hyper-charged person that was able to lift up the energy of an entire room!! Where was that me? Where did it go? I wanted to find him and bring him back! And he most certainly has done that.So how has he created his amazing coaching business the way he has?And how has past those first year obstacles that make growing a business so difficult if you don't do things in the right order?Well lets find out as we bring onto the show to start joining up more dots, with the one and only Simone VicenziShow HighlightsDuring the show we talked about such weighty subjects as:How he grew his business and developed a team that has helped him get his life back, instead of ploughing through seventeen hour days.Simone reveals how he started his own business, by attending seminars which gave him an insiders view of what others were doing.Why it is so important to delegate the outcomes before you ever think about delegating the tasks to a VAand lastly...............Simone reveals the moment his wife wanted to go to New York without him, and how it changed the way he thought of business.

In this episode I talk about how we are scaling operations at GTeX online. Want more? www.gtex.org.uk

Simone Vincenzi, known as The Experts Strategist, is the co-founder of GTeX, Forbes, Entrepreneur Magazine and Huffington Post contributor, TEDx speaker, Host of the podcast Explode Your Expert Business, author of 3 life-changing books. Simone is passionate about turning experts into authorities using his signature EXPLODE YOUR EXPERT BIZ programs. Every year he speaks in front of more than 5,000 business leaders, which included sharing the stage with Les Brown, Dr John DeMartini, Simone Sinek, John C. Maxwell and many more. He has also organised over 600 events, including Gary Vee speaking from York Hall boxing ring, and he is currently speaking in more than 200 stages every year. Following his passion for making an impact on the younger generation, he also partners with the largest youth organisations in the UK, helping them deliver more effective courses and training. Simone cannot live without 3 things: Speaking, basketball and playing his Didgeridoo.

Starting as a restaurant manager Simone molded his path to a life and business he loves. Simone shares how he made it as an entrepreneur with little initial knowledge about business.

Jane Ferguson: Hi everyone. Welcome to episode 20 of Getting Personal Omics of the Heart, the podcast brought to you by the Circulation: Genomic and Precision Medicine Journal and the American Heart Association Council on Genomic and Precision Medicine. I'm Jane Ferguson from Vanderbilt University. It's September 2018 and let's dive straight into the papers from this month's issue of Circulation: Genomic and Precision Medicine.                                 We're starting off with some pharmacogenomics. Bruce Peyser, Deepak Voora and colleagues from Duke University published an article entitled, "Effects of Delivering SLCO1B1 Pharmacogenetic Information in Randomized Trial and Observational Settings."                                 Although statins are generally well tolerated, 5-15% of patients taking statins for LDL lowering and cardiovascular protection end up developing statin associated muscular symptoms. Because onset of muscular symptoms associated with discontinuing statin use, as well as increased cardiovascular morbidity, there is a clear need to identify ways to prevent or reduce symptoms in these people. Variants affecting statin related myopathy have previously been discovered through GWAS, including a variant in the SLCO1B1 gene, which also has been shown to relate to statin myalgia and discontinuation of statin use. The risks appear to be greatest with simvastatin, indicating the people at risk of muscle complications may do better on either low-dose Simvastatin or another statin. However, there's still some uncertainty surrounding the risks and benefits of various statins as they pertain to risk of muscular symptoms.                                 The authors have previously shown that pharmacogenetics testing led to increased number of people reporting statin use, but effects of pharmacogenetic testing on adherence, prescribing, and LDL cholesterol had never been tested in a randomized control trial. In this study, they randomized 159 participants to either genotype informed statin therapy or usual care, and then followed them for months to eight months.                                 25% of participants were carriers of the SLCO1B1 star five genotype. The authors found that statin adherence was similar in both groups, but gene type guided therapy resulted in more new statin prescriptions and significantly lower LDL cholesterol at three months, and levels that were lower but no longer significantly different at eight months.                                 In individual's randomized to usual care who then crossed over to genotype informed therapy after the trial period ended, there was an additional decrease in LDL cholesterol. Overall, genotype informed statin therapy led to an increase in re-initiation of statins and decreases in LDL cholesterol, but did not appear to affect adherence.                                 The authors also examined the effects of commercial genetic testing for SLCO1B1 variants in an observational setting by looking at over 92000 individuals with data available in the EHR. They found the people who receive genetic testing results had a larger drop in LDL cholesterol compared to untested controls. Overall, the study indicates that carriers of the SLCO1B1 risk variant may benefit from genotype informed statin therapy, while for non-carriers receiving their results may has limited effects.                                 If you want to read more on this, Sony Tuteja and Dan Rader from UPenn wrote an editorial to accompany this article, which was published in the same issue.                                 We're staying on the topic of statins and LDL for our next paper. This article comes from Akinyemi Oni-Orisan, and Neil Risch and colleagues from the University of California and is entitled, "Characterization of Statin Low-Density Lipoprotein Cholesterol Dose-Response Utilizing Electronic Health Records in a Large Population-Based Cohort."                                 They were interested in understanding what determines variation in statin induced LDL reduction, particularly the genetic component, and they used a large EHR derived data set, the Kaiser Permanente Genetic Epidemiology research on adult health and aging cohort to address this important question. An EHR dataset does have intrinsic limitations, but also has some clear strengths, not only as a readily available and cost-effective data source for large sample sizes, but also because it reflects real world clinical care in diverse individuals, which is not always well represented within the selective constraints of a randomized trial.                                 There were over 33000 individuals who met their inclusion criteria. To account for differences in potency between different statins and doses, the authors generated a defined daily dose value, with one defined daily dose equal to 40 milligrams per day of Lovastatin. The slope of the dose response was similar across statin types and across different sex and race or ethnicity groups. But there were differences by statin type in the response independent of dose, as well as differences in absolute responses by sex, age, race, smoking, and diabetes.                                 Based on these differences, the authors revised the defined daily doses and they highlight how previously defined equivalencies between different statins may not be accurate. They found that individuals with East Asian ancestry had an enhanced response to therapy compared with individuals of European ancestry.                                 The authors identified related individuals within the data set and the estimated heritability of statin response using parent-offspring and sibling pairs. They found only modest heritability, indicating that non-genetic factors may be more important in determining variability in statin response. Overall, this large single cohort study adds to our knowledge on determinants of statin response and raises further questions on the relative effects of different statins and doses within patient subgroups.                                 Okay, so now let's talk about GWAS and Athero. Sander van der Lann, Paul de Bakker, Gerard Pasterkamp and coauthors from University Medical Center Utrecht published a paper entitled, "Genetic Susceptibility Loci for Cardiovascular Disease and Their Impact on Atherosclerotic Plaques."                                 Over the past decade, genome-wide association studies in large cohorts have been very successful in identifying cardiovascular risk loci. However, relating these to subclinical disease or two mechanisms has been more challenging. The authors were interested in understanding whether established GWAS loci for stroke and coronary disease are associated with characteristics of atherosclerotic plaque, the idea being that some of the risk loci may alter disease risk by determining the development and stability of plaque. They identified seven plaque characteristics to study and histological samples, including intraplaque fat, collagen content, smooth muscle cell percentage, macrophage percentage, calcification, intraplaque hemorrhage, and intraplaque vessel density.                                 They selected 61 known loci and examined association of those SNiPA with black phenotypes in over 1400 specimens from the athero express biobank study. Out of the 61 loci, 21 were associated with some black phenotype compared with zero of five negative control loci, which were chosen as established GWAS loci for bipolar disorder, which, presumably, should share limited mechanistic etiology with plaque. They used the software package VEGAS to run gene-based analyses. They also assessed SNiPA relationships with gene expression and methylation in multiple tissues derived from two independence Swedish biobanks, which included atherosclerotic arterial wall, internal mammary artery, liver, subcutaneous fat, skeletal muscle, visceral fat, and fasting whole blood.                                 One CAD locus on chromosome 7q22 that survived correction for multiple testing was associated with intraplaque fat, and was also an EQTL for expression of several genes across multiple tissues. In addition, it was also a methylation QTL.                                 The authors focused on this locus and looked at correlation of expression within the LDL receptor and noted associations with HDL and LDL cholesterol in the global lipids genetics contortion data, which suggests that this locus may have a role in the metabolism. At this locus, the HBP1 gene expressed foam cells may be an interesting candidate as a causal gene in determining plaque-lipid accumulation and cardiovascular risk.                                 So next up, we have a paper that is also about athero and is coauthored by many of the same group as did that previous study. So yeah, this group's productivity is kind of making the rest of us look bad this month. So Martin Siemelink, Sander van de Lann, and Gerard Pasterkamp and their colleagues published, "Smoking is Associated to DNA Methylation in Atherosclerotic Carotid Lesions."                                 Okay. So I think one of the few things we can all definitely agree on is that smoking is bad. But, does smoking exert any of its cardiovascular damage by altering within atherosclerotic plaques? That's the question this group set out to answer.                                 They carried out a two-stage epigenome-wide association study, or EWAS, with discovery and replication of differentially methylated loci with tobacco smoking within carotid arteriosclerotic plaques of a total of 664 patients undergoing carotid endarterectomy and enrolled in the arthero-expressed biobanks study. In discovery, they found 10 CpG loci within six genes that associated with smoking. Four of the CpG loci replicated. These four loci mapping within six genes showed reduced methylation in current smokers compared with former or never smokers.                                 However, there was no difference in specific plaque characteristics based on methylation at any of the four loci. There was also no significant difference in plaque gene expression at these loci based on smoking status. However, a SNiPA at a nearby locus located in the 3' UTR of the PLEKHGB4 Gene was associated with methylation at AHRR, and was a [inaudible 00:09:58] QTL for PLEKHGB4 of expression but not a AHRR expression. The authors speculate that PLEKHGB4 may co-regulate AHRR expression. The authors also examined blood methylation in a subset of the same subjects, and they were able to replicate previously identified CPG sites associated with smoking.                                 This is a really complex area, and it's hard to identify mechanisms and causality from these multiple layers of data, but the authors demonstrate the importance of using disease relevant tissues to start to understand how environmental factors interact with genetics and other underlying physiology to modify methylation and function within the vasculature.                                 Our final full-length research paper this issue from Brian Byrd and colleagues Michigan, is actually the subject of our interview today. So I won't go into too much detail on it right now, but keep listening for an interview with Brian about their paper, "Human Urinary mRNA as a Biomarker of Cardiovascular Disease: A Proof-of-Principle Study of Sodium-Loading in Prehypertension."                                 Our review article this month is about the "Dawn of Epitranscriptomic Medicine" from Konstantinos Stellos from Newcastle University and Aikaterini Gatsiou from Goethe-Universität Frankfurt. In this paper, they're taking us to the next level beyond just RNA, but towards RNA epigenetics. Given the large number of possible modifications that can and are made to RNA during RNA name metabolism, there's huge potential to gain a new biological and mechanistic understanding by studying the RNA epitranscriptome. I think we'll ignore this at our peril. So if you need to catch up on this new field, this comprehensive review will get you right up to speed.                                 Moving on, our research letters are short format papers that allow authors to present focused results. These are also a great avenue to submit findings from replication studies that might not necessitate a full-length paper. So if you have some data from a replication study that you've been procrastinating writing up, a short research letter is a great format to consider.                                 This month, Bertrand Favre, Luca Borradori and coauthors from Bern University Hospital published a letter entitled, "Desmoplakin Gene Variants and Risk for Arrhythmogenic Cardiomyopathy: Usefulness of a Functional Biochemical Assay." The desmoplakin is essential for the cell-cell adhesion complex's desmosomes. Mutations in this gene have been associated with a wide range of phenotypes, including some in skin and hair, but also in heart, which can manifest arrhythmogenic or dilated cardiomyopathy. This protein anchors intermediate filaments, so mutations that alter binding to intermediate filaments may pathogenicity.                                 The author selected seven reported amino acid altering mutations in desmoplakin, and they screened for effects on binding using a novel fluorescence binding assay. They found that three of the seven mutations had a clear impact on binding. This assay is a novel way to assess functional impact of desmoplakin variants, and may be useful to inform the severity of future phenotypes in individuals carrying a desmoplakin mutation.                                 Finally, if you want to stay up-to-date on the genetics of aortic disease and Marfan syndrome, you can find a letter from Christian Groth and colleagues and an author response from Norifumi Takeda and colleagues regarding their previously published paper on impact of pathogenic FBN1 variant types on the progression of aortic disease in patients with Marfan syndrome.                                 I am joined today by Dr. Brian Byrd from the University of Michigan, who is the senior author on a Manuscript published in this month's issue, entitled, "Human Urinary mRNA as a Biomarker of Cardiovascular Disease: A Proof-of-Principle Study of Sodium-Loading in Prehypertension."                                 So welcome Brian. Thanks so much for coming on the podcast. Brian Byrd:          Thank you for having me. Jane Ferguson: So before we get started, could you give a brief introduction of yourself to the listeners and maybe tell us a little bit about how you got into the field? Brian Byrd:          Absolutely. So I am a cardiologist and a physician scientist. I'm an assistant professor at the University of Michigan, where I have a laboratory engaged in clinical investigation. My background is that I did my Internal Medicine Residency at Vanderbilt University. And after I finished residency, I entered Nancy Brown's lab. She's the Chair of Medicine at Vanderbilt, as I know you're aware. And she had a laboratory focused, and still does have a laboratory focused, on the investigation of high blood pressure, with a lot of focus on understanding high blood pressure as it occurs in humans. And I got a Master of Science degree in clinical investigation while I was in her lab, and we did some work on a number of topics related to the renin-angiotensin-aldosterone system, which has been a long-standing interest of mine ever since then.                                 So, at the same time, I was learning how to take care of patients with very complex blood pressure problems, who required three, or four, or five, or six blood pressure medications, in some cases, to control. And it's with that background that I became very interested in the science that underlies treatment-resistant high blood pressure in people and what we might be able to do about that. Jane Ferguson: Wow. Nice. Yeah and I think that background of sort of the combination of clinical and experimental is really nice and really important. I think your paper actually exemplifies that really nicely, so using humans but also some basic science techniques and combining them to really have a very patient focused instead of mechanistic interrogation.                                 So as I mentioned, you just published this really nice manuscript using urine as a source of mRNA biomarkers, which has relevance to hypertension and potentially also to other diseases. But before we get sort of too much into the weeds on the specific details, for any of our listeners who didn't get a chance to read your paper yet, maybe you could briefly summarize what you did? Brian Byrd:          Okay, so the general overview of what we were interested in was that the patients who have treatment resistant high blood pressure tend to have a lot of activation of a receptor in the kidney called the mineralocorticoid receptor. And this receptor helps control salt in bladder in the body. Obviously the amount of salt in the blood stays very, very homeostatic, but we if eat more salt one day then the next and there needs to be a system to help regulate the homeostasis. And so, you waste more or less salt in the urine depending upon how much sodium you're taking in.                                 And one of the functions of the mineralocorticoid receptor is to control this salt and bladder regulation or to fine tune it anyway. And the reason we know that that's an important receptor in patients with treatment-resistant high blood pressure is because of a series of studies done by David Calhoun and Brian Williams and others, showing that mineralocorticoid receptor blockers, or antagonists, are very effective in the treatment of tough to control high blood pressure.                                 And so, we had some insight that there would be something interesting to study there, and one of the things that we knew was that the mineralocorticoid receptor is a ligand activated transcription factor. So when it gets activated by it's ligan which canonically is a steroid hormone from the adrenal gland aldosterone, the receptor, which is in the cytoplasm, ordinarily dimerizes and translocates to the nucleus, where it controls the regulation of a number of genes. We also were aware that cells secrete RNA, and others had the idea that it might be inside vesicles because there's a lot of ribonuclease and biofluids. And you would think it might be difficult to pass the RNA if it were sort of naked as it were.                                 And it turns out that that's right. If you, for example, introduced synthetic RNAs into biofluids, the RNAs will be gone very quickly in a matter of seconds. So, we had this idea that we might be able to look at RNA that was being secreted by cells probably in vesicles, and assay the activity of the receptor potentially. We weren't sure if that was going to be possible or not.                                 One of the things we did was we used part of the available data to look at the transcriptome of vesicles in the urine that had been isolated from 3300 milliliters of urine by ultracentrifugation [inaudible 00:18:57]. Jane Ferguson: So it's a big centrifusion. Brian Byrd:          Exactly. Jane Ferguson: Like you [inaudible 00:19:00] Brian Byrd:          It must have been some project. So that was the work of Kevin Miranda and colleagues, and we were able to compare that transcriptome to the transcriptome of human kidney cortex samples from the GTEx project, which a large consortium focused on human transcriptomics.                                 And that was sort of the first part of what we presented in this paper, and the second thing that we did was we looked within a crossover study in a collaboration with Scott Hummel, one of my close collaborators here at the University of Michigan. We looked at individuals who had been put on a low salt diet activating renin-angiotensin-aldosterone system and causing more activation of the mineralocorticoid receptor. And then, those same individuals underwent saline infusion, so salt loading, and we knew that that would suppress the renin-angiotensin-aldosterone system. And we measured the [inaudible 00:20:02] measures of the renin-angiotensin-aldosterone system, but we also took the urine samples that had been recently banked from that experiment and we centrifuged them to try to palette the cells. We took the supernatant and we extracted RNA after trying to enrich for extracellular vesicles.                                 And with that approach, we measure targets that we thought would be regulated my the mineralocorticoid receptor, as well as some things that we did not think would be regulated by mineralocorticoid receptor. So that's the general overview of what we undertook. Jane Ferguson: Great. Right. So it's very cool. I guess we can break it down into sort of the two different parts, because I think it was a really nice examples of using public data to sort of start addressing your question and then actually doing a human experiment. But so for the GTEx data and the urinary data, you looked at few different tissues, right? And was kidney the one that you were thinking upfront would sort of most likely to correlate, or were you also looking at bladder and other sort of tissues that could potentially be of relevance to urine? But what sort of the ... I guess sort of tell me more about those different tissues that you looked at and what you found and what surprised you or not. Brian Byrd:          Great question. So, the kidney was on our minds from the outset. We knew that Mark Knepper at the National Institute of Health had published in the [inaudible 00:21:25] National Academy of Sciences back in 2004 that there are urinary extracellular vesicles. And he had found proteins that are very characteristic of the aldosterone sensitive distal nephron, that part of the kidney that we're interested in, embedded in the vesicles.                                 So we became quite interested in the idea that it seemed that there was likely a population of vesicles in the urine that is of kidney origin. And that's not to say that there weren't also plenty of vesicles from other origins as well, and there could very well be RNA that is not vesicle enclosed, but is rather ribonucleic protein bound or even bound to other carriers potentially. That could be there as well, and it's possible that the origin of those things could be any number of tissues. I don't really think that we know yet where the possible tissue origins are.                                 But we were curious to know ... I guess the direct answer to your question is we thought from the outset that we probably would find some sort of signal related to the kidney. But we wanted to also consider the possibility that our findings were not very specific to the kidney. And so we thought that the brain would be an interesting negative control. If we say very high correlation with the brain, it would suggest that maybe what we're looking for is a signal that's not really coming from the kidney.                                 And we also wanted to look at the bladder just to try to understand whether or not the signals that we're detecting could be coming from the bladder. It's certainly true that some aspects of the system that we're interested in are present in the bladder, so I wondered whether that might even serve as a signal amplifier for what we were looking for since there's, presumably, quite a bit of bladder tissue right around the urine. It might be contributing vesicles.                                 So that's sort of the rationale for why we looked at those things. Jane Ferguson: And you found mostly enrichment for kidneys. So sort of I guess what you were hoping to find came true? That actually there was sort of evidence that even though there may be contribution from other tissues, that really kidney seem to be the predominant contributor to the expression of the genes in the urine. Brian Byrd:          I think there's a lot of truth to that. One of the things I would say is we found high correlation looking across all genes. But it occurred to us ... As soon as we thought that, we realized, wait a second, that could be driven by ubiquitously expressed genes. Housekeeping genes.                                 So we really wanted to stratify our analysis by things we thought would be expressed in the kidney as well as things that we thought would be ubiquitously expressed to make sure that we could see signals that correlate ... That the transcriptome of the kidney, per se, had a good correlation with those same in terms of the abundance of the gene counts or recounts. They said it was similar to what was in the vesicles.                                 And so, we looked in the literature, and we found that a group had already established a number, 55 genes actually, that were highly kidney enriched as well as over 8000 genes that were ubiquitously expressed. And so we started the analysis from this perspective of the stratification. We thought that was a very important aspect of the analysis. And it's definitely true that if you look at our findings with respect to the kidney enriched genes, as you might expect, they correlate quite well with what is in the urinary extracellular vesicles compared to the kidney cortex.                                 You look at the brain as you might expect the expression of those kidney enriched genes is not well correlated with what's happening in the urine. And then, with respect to the bladder, it's sort of somewhere in between. Jane Ferguson: Okay. Interesting. So I know that some people look at small non-coding RNAs in urine, but you were mostly focused on mRNAs. Is that right? Brian Byrd:          That's right. I thought of this as sort of frontier, something that I knew from some early publications was probably measurable. But I didn't know what it would signify, if anything, with respect to physiology. And I knew that there were quite a few papers about micro RNAs and I wanted to do something a little bit innovated, partly.                                 But the main reason that I was interested in the RNAs was because I could relatively easily tie those to the existing literature from animal models. Preclinical animal models and cell culture studies showing what happens when the mineralocorticoid receptor's activated. That was really the driving reason that I was interested in the RNA. Because if you think about what is the approximate event that might be a readout for activation of a new growth hormone receptor like the mineralocorticoid receptor, it's really the transcriptional events that happen when the receptors translocates to the nucleus and serves its ligan activated transcription factor role. Jane Ferguson: Right. So, [inaudible 00:26:43] sort of the first part of analysis, you saw these really nice correlations between expression and kidney and in urine. And then, a lot of the times when you tried to publish that kind of thing, people are like, "Okay, so what? So you didn't do any intervention. We don't really know what that means."                                 But I like that you took it to the next step and you did sort of a human intervention experimental model. So tell me more about that model and how that worked. Brian Byrd:          Right. Well, I'll just mention also that the work that was done in terms of RNA [inaudible 00:27:14] was done in collaboration with Mark Bertini in Italy as well as Dr. [inaudible 00:27:19]. They were fundamental to getting that work done.                                 With respect to the collaboration with Scott Hummel, one of my colleagues here at the University of Michigan, what we did in that setting was to look at whether or not we could identify within these urinary mRNA signals that are in the supernatant in the urine, whether we could identify changes in physiology. That was the question that was of greatest interest scientifically.                                 And for a very practical or blind perspective, the question was could we detect the activation of the receptor that might determine whether or not people should get a certain medication. Of course, we're not saying that that's an established fact yet, but this is sort of concept, that there's something here to explore further.                                 And so, what we found was that a number of genes that are regulated by the mineralocorticoid receptor, including genes encoding the subunits of the amiloride-sensitive epithelial sodium channel that regulates the salt that I was talking about earlier. We found that those genes changed with sodium loading in terms of their abundance in the expected direction.                                 We also found that several of the assays that we made changed ... I'm sorry. That they correlated with the serum aldosterone concentration. So the concentration of the ligan for the receptor whose readout we were looking for. And we also noticed an inverse correlation with urinary sodium excretion, which is what we would expect if we really identified a readout of the mineralocorticoid receptor's activity.                                 So this study supported the idea that we have identified a way to measure this nuclear hormone receptors activity in living humans. Jane Ferguson: Right. Which is really nice. So there's probably a huge amount of extra things you could do with this, some sort of different ways you could look at it. So how did you pick the time point? So, I suppose when you think about it, I mean the genes, they're transcribed and then that takes a little bit of time, and then it takes a little bit of time for that to sort of make its way into the urine and to be excreted.                                 So how did you decide on sort of what time points to use, and do you think you would see the same things or different things [inaudible 00:29:39] if you did repeated sampling or if you looked at different time points? Brian Byrd:          That's a fantastic question. So this was a study that had already been completed, and I had mentioned to Scott what we were working on. And he said, "You know, we have these samples from this study and it might be possible for us to collaborate."                                 So, we didn't get to pick the timeframes. Jane Ferguson: Right. Brian Byrd:          So, that's a great point. And what I would say is that, as you can imagine, we're very focused on exactly the questions you're asking now. What about sort of signal refinement? What about the chrono-biology of these signals, and how do we understand when we see what in the urine?                                 So, I'm actively pursuing those questions. Jane Ferguson: Right. So, I know as well, there was quite a lot of sort of technical challenges I think to doing this work. Sort of getting to be even able to amplify and get a signal from these RNAs that are really present, sort of pretty low abundance in urine compared to tissues or biofluids that we're used to working with.                                 So tell me maybe a little bit about that process and sort of how much optimization was required to get these essays to work? Brian Byrd:          Great question. So, I had known [inaudible 00:30:58] since 2014 when I took a course on isolation of extracellular vesicles in Heidelberg, Germany. And I had talked to him at a meeting in Washington DC, and I had mentioned what we were trying to do. And he said, "You know, if you were trying to do that, you might want to consider preamplification." You know, using something like 15 cycles of preamplification. And he was willing to share that protocol that he had with me, because they were interested in similar issues. So, I was able to use that protocol to evaluate these gene targets in the urine. And so that was immensely helpful.                                 And the other thing that we did was we used locked nucleic acid probes to try to increase the sensitivity and specificity of our assays. Finally, we just tried to use good logic in the design of the assays. So we were concerned that the RNA might be fragmented, so where it was possible to do so within the design constraints that I'll mention in a second, we made multiple assays per gene target just in case this was fragmented. Which makes the analysis a little more complicated, but I think it was probably the right thing to do, given the state of knowledge that we had then.                                 And one of the other things we did was we made sure that the primers either ... Within a primer, there was an intron or between the primers there was an intron, so that if we actually did try to amplify DNA, abundant amounts of DNA, with those primers just to make sure that our theorizing about the inability to amplify things was actually factual. And that turned out that we couldn't amplify anything at 40 cycles with those.                                 So, we spent a lot of time thinking about how not to get fooled, but also to have adequate signal detection. And have included in the supplement quite a bit of information about the technical merits of the assays and showing how close the technical replicates were. They tended to be very, very similar to one another. We didn't see a signal in every urine sample for every participant at both time points, and I think that was interesting to me about that there tended to be a very binary result, so that you'd either see three technical replicants for the QPCR assays, our QPCR assays that were extremely similar to each other, or you would see no CT value detected.                                 [inaudible 00:33:47] That these were valid assessments of very low copy numbers. Jane Ferguson: Right. And that's probably related to up front of what happens to urine right after it's collected and stored, or during that RNA extraction. But it seems like once you've got RNA, then downstream assays were sort of ... They held through, but I guess ... I mean, and you obviously didn't have necessarily a huge amount of control over how these urine samples were collected. So it's kind of nice that you were able to see something even though these were collected possibly in a way that was not optimized for preserving RNAs.                                 But do you think those ... Are there ways that you could make this even sort of more streamlined and better as far from the get go of how you collect the urine, whether you could be extracting stuff right away? Is that anything you sort of looked into of how this could be improved? Brian Byrd:          That's really been the focus of the labs work since we completed that project, is sort of understanding how would we do this in a prospective study in the best possible way so that the results are highly repeatable, that we get a CT value in everybody so that we're really ... I mean, as you can imagine, that actually has something to do with the input volume of urine that you use. So if you have too little input volume, then you won't be able to detect the targets that you might be interested in every person.                                 However, if you have more, then you can do more with that. But then you have to think about how you're going to deal with the larger volumes of urine. There are lots of questions that we've been interested in related to extract the RNA and the stability of the RNA. And so we have done some experiments of that type, and we continue to work in that area. And I do think that those questions you're asking are the right questions with respect to next steps. Jane Ferguson: All right. So you looked at sort of specific targets, which I think made a lot of sense. Sort of this proof of principle. But do you think this would work on a transcriptome wide level? I mean, could you look at all the genes, or do you think that's just sort beyond the possibility right now given sort of the RNA fragmentation and how you have to sort of amplify it before being able to detect anything? Brian Byrd:          I think it's possible. So the group that had preceded our work with 3300 mils of urine, isolating the vesicles from there, eight have showed that that's something that can be done. The question that's of interest to me is does it actually require such large volumes of urine? And I think the answer to that question is going to be no from what we're overseeing so far.                                 And so, we're thinking along exactly the lines that you are. And certainly some of the feedback we've gotten as we've discussed this project with people is, "Hey, could you look at everything rather than picking targets at [inaudible 00:36:41]."                                 I think there's advantages and disadvantages. I think we chose based on prior knowledge in a way that was rational. But at the same time, it may turn out that there are many things about activation of the mineralocorticoid receptor in humans, especially in the living in-tact human, that don't exactly mirror what's found in rabbits, rats, mice or cells, which are really the systems that have been evaluated the most thoroughly in the past.                                 So I'm very interested in exactly what you're proposing. Jane Ferguson: Yeah. I mean, I think it's exciting because it's obviously relevant for hypertension, but potentially a lot of other conditions, to be able to look at that sort of dynamic change. So I think it's really exciting. It's very cool. Brian Byrd:          And I appreciate your asking about this study. We were excited to do this work and very, very excited to see where we can in the future with this. And I agree with the point you were making, that here we've gone from a rather specific application driven question and we've, I think, made some insights that are probably useful outside the application that we had in mind. And it may turn out that the application where this is the most important is not even the one that we considered in the first place at all.                                 And so I'm pleased by that. I'm pleased by the fact that I think in a sense we're working in what Donald Stokes described as pasture's quadrant, which is a sense that the work is driven both by curiosity and by an intent to use the results. Jane Ferguson: Right. Brian Byrd:          And so that's really what gets me out of bed in the morning, is working that exact space. So that's what we were glad to have done and continue to do. Jane Ferguson: Yeah. No, I think it's grea.t and I feel like a lot of people will read this paper and be like, "Hey, I have urine stored in the freezer. What can I do with this now?" Brian Byrd:          Contact me. Let's talk. We'll see what we can do. But we certainly tried to describe the methods in such a way that people could easily follow in our footsteps if they want to apply these methods. Jane Ferguson: Yeah. Now having read through them, I think that ... Really thorough. I really liked the sort of attention to detail. It was definitely one of those ones where I was like, "Oh yeah. I can see exactly how I could do this if I wanted to. So I think that was great. Brian Byrd:          Thank you. Jane Ferguson: So yeah. Congratulations on the paper. Really nice work and thanks so much for talking to me. Brian Byrd:          Thank you. It was a delight. Jane Ferguson: That's it from me for September. If you haven't had enough yet, you can access all the papers online and you can choose to digest the papers in video format. Available on our website or the Circulation YouTube channel. Thank you for listening and subscribing. I look forward to bringing you more next month.  

In this episode I share our sales philosophy that every sales person in GTeX™ must have.

Simone Vincenzi (founder of GTEX) is an entrepreneur, speaker and host of the Explode Your Biz podcast. Today, Simone will share how you can move forward in your public speaking and your business by being accountable to your clients. More at www.bemovingforward.com. Follow us on Facebook, Twitter, LinkedIn @Bemovingforward The Corporate Cliches Adult Coloring Book is available on Amazon and Barnes & Noble online: Just go to Bit.ly/corporatecliches

 Today We Are Working Together To Learn What It Takes For Growing Together Exponentially aka GTeX:  Growing Together Exponentially, known as GTeX, was founded in April 2013 by Simone Vincenzi @GTeXuk and Ben Shorter, two passionate personal development coaches, who were inspired to create a community of empowered individuals, through the sharing of tools and techniques to rapidly increase results in all areas of your lives. Now known as one of London’s leading personal development communities, GTeX has grown into a sustainable business that has empowered 100’s of people over the last 12 months to rapidly achieve results in all aspects of their lives by sharing the latest tools and techniques at our events, as well as our one to one and group coaching programs.   On This Episode You Will Hear:[spp-timestamp time="00:30"] Introduction [spp-timestamp time="56:00"] Final Words   If You Don't Grow, You Die! - Quote Simone Vincenzi [spp-tweet tweet="If You Don't Grow, You Die! Listen for Growing Together Exponentially with Simone Vincenzi! @GTeXuk "] Watch On YouTube:[youtube https://www.youtube.com/watch?v=shz9hJiv8Kc] Links and Resources: GTeX UK (https://gtex.org.uk/) GTeX UK on Twitter (https://twitter.com/GTeXuk) GTeX on YouTube (https://www.youtube.com/channel/UCEbTTMRkXtvSSx5vaXmCTGA) GTex on Facebook (https://www.facebook.com/GrowingTogetherExponentially) Simone Vincenzi (https://www.linkedin.com/in/simone-vincenzi/)   People Mentioned:TBD   Final Words:FINAL WORDS…Pending   Action Steps:Please Subscribe and Submit an iTunes Show Rating & Review (https://itunes.apple.com/us/podcast/livethefuel/id1150969758?mt=2) . The more reviews, the more people can find us. We love 5 Stars too! Join our private Facebook Group community called THE FUEL TANK (http://livethefueltank.com/) . Visit our  Resources (https://livethefuel.com/resources)  page and a reminder to LIVE-THE-FUEL my friends! (https://gtex.org.uk/about-us/)

Are your seminars converting high ticket offerings like they should? Guest Simone Vincenzi shares two models you can use to make your seminars profitable and drive clients to your high ticket offering. In this episode of The 10 Factor find out: Why Simone had to spend a winter living on the streets of London. The difference between a three day and four day event. The big investment Simone made that put him 30K in the hole. How to create an environment so the audience implements real results on the spot. Why you shouldn’t confuse GTeX with a Tech Company. Reach out to Simone Vincenzi: To access Simone Vincenzi’s Ultimate Selling From the Stage Checklist go to: www.Gtex.org.uk Website: www.Gtex.org.uk Facebook: https://www.facebook.com/simonegtex/ Explode Your Coaching Biz Show: https://itunes.apple.com/us/podcast/explode-your-coaching-biz-show/id1065552208?mt=2 @simonegtex twitter @gtexuk instagram @simonegtex snapchat JOIN our Community - become part of the family It's growing and it's growing fast. To find out more and apply drop Tim an email. He'll reach out to you personally to see if you are a good fit. tim@the10factor.com Help Support our Show: Reviews Do Matter. We love to read our subscribers 5-star reviews on iTunes. Get a bonus thank you if yours gets read on the show. Leave us an honest review Find us on iTunes to *subscribe* and leave a 5-star review Find "The 10 Factor" on iTunes Submit questions or comments - we read and answer questions right on the show. tim@the10factor.com Connect with Tim: Facebook: https://www.facebook.com/the10factor/ Instagram: @TimMeuchel Twitter: @TimMeuchel email: tim@the10factor.com Upcoming Episodes and Guests: Subscribe today so you don't miss any of our twice weekly episodes.  030: Mark Nendick on Maintaining a Healthy Lifestyle While Raising a Family 031: Sean Douglas on Your Big "Why" and What to Do With It 032: Mark Lack on Building a Rockstar Personal Brand - - -  UNTIL NEXT TIME: Tim Meuchel - The 10 Factor, signing off

Simone Vincenzi, Co-Founder of GTeX , Podcast host , Speaker. GTEX help speakers and coaches to dominate. Helping them creating online or live events that sell without being a douchebag. Job Title: Co Founder of GTeX , Podcast host , Speaker Name: Simone Vincenzi Instagram Handle: gtex Name of Business: GTeX Website: https://gtex.org.uk/

My guest today, on the Steve Jobs inspired Join Up Dots free podcast interview is a man with a similar career path to myself. And a man who also has a desire to see the world flourish, and help individuals across the globe to grow exponentially, becoming their greatest versions. But of course to get to that point, you truly have to go through a journey of discovery yourself, to find your inner skills that make you unique and valuable to everyone. And it would be fair to say that just a few years ago, our guest was many many steps away from discovering their purpose in life. He was working in a restaurant in Notting Hill as a waiter, as he had been doing since the age of 15, and had certain mental restrictions should we say, that were holding him back from creating his dream life. He didn't have a degree and his level of English was not quite enough to understand the self development literature that he was starting to immerse himself in. As he says, “Just three years ago I was facing a very difficult moment of my life. I knew I had to change but I didn't know what to do. I felt lost, like wondering in a circle in a dark room with no exit sign and this wasn't me!! I have always been the uber-positive-hyper-charged person that was able to lift up the energy of an entire room!! Where was that me? Where did it go? I wanted to find him and bring him back! So he took the plunge and signed up for an extensive two day coaching course in London, even though he had nowhere to live, and started working on himself everyday. He built that personal belief that he had inside him what was required to create a succesful business and little by little saw his life and his finances change. He first set up his coaching company Your Power to Shine Ltd and then a personal development events company, GTeX and was on his way. And now with a flourishing public speaking, coaching and development, and didgeridoo business (well the last one isn't a business but is very impressive) he seems to be having the time of his life. So when did he truly switch the switch of belief and start making the bold decisions that make such a difference moving forward? And does he look back at himself and think “You were always ready to find the power to shine” you just had to start? Well lets find out as we bring onto the show to start joining up dots with the one and only, or as they say in Italy la sola e unica, Simone Vicenzi Show Highlights During the show we discussed such weighty topics with such as: How he recalls being held back by his personal self image at the very beginning and battled as he felt he was being made to conform to the normal. How he went from being the youngest manager in a Michelin Star Restaurant, to creating his coaching business in just three years. And it was all down to building his network! Why he believes that it is a great learning experience to handle everything in the early stages of creating a business, but start handing things off as soon you can to focus on what you do best. and lastly…. The strangest stories involving body waxing, organic vegetables, ex-girlfriends, hitchhiking, and how to get free rent in London that you will ever hear. 

My guest today, on the Steve Jobs inspired Join Up Dots free podcast interview is a man with a similar career path to myself. And a man who also has a desire to see the world flourish, and help individuals across the globe to grow exponentially, becoming their greatest versions. But of course to get to that point, you truly have to go through a journey of discovery yourself, to find your inner skills that make you unique and valuable to everyone. And it would be fair to say that just a few years ago, our guest was many many steps away from discovering their purpose in life. He was working in a restaurant in Notting Hill as a waiter, as he had been doing since the age of 15, and had certain mental restrictions should we say, that were holding him back from creating his dream life. He didn't have a degree and his level of English was not quite enough to understand the self development literature that he was starting to immerse himself in. As he says, “Just three years ago I was facing a very difficult moment of my life. I knew I had to change but I didn't know what to do. I felt lost, like wondering in a circle in a dark room with no exit sign and this wasn't me!! I have always been the uber-positive-hyper-charged person that was able to lift up the energy of an entire room!! Where was that me? Where did it go? I wanted to find him and bring him back! So he took the plunge and signed up for an extensive two day coaching course in London, even though he had nowhere to live, and started working on himself everyday. He built that personal belief that he had inside him what was required to create a succesful business and little by little saw his life and his finances change. He first set up his coaching company Your Power to Shine Ltd and then a personal development events company, GTeX and was on his way. And now with a flourishing public speaking, coaching and development, and didgeridoo business (well the last one isn't a business but is very impressive) he seems to be having the time of his life. So when did he truly switch the switch of belief and start making the bold decisions that make such a difference moving forward? And does he look back at himself and think “You were always ready to find the power to shine” you just had to start? Well lets find out as we bring onto the show to start joining up dots with the one and only, or as they say in Italy la sola e unica, Simone Vicenzi To hear more from Simone Vicenzi go to: Podcast: Explode Your Coaching Biz Show Description: The podcast for coaches, trainers and speakers who want to make an impact in the world building a successful biz along the way.

A lot of men struggle with body image or eating disorders which often fly under the radar. There are also people who have trouble finding their purpose in life. These are two things that Simone Vincenzi, Co-Founder of GTex knows about, and how to turn it around. In this frank discussion, Simone highlights important areas and what was his own breaking point, and outlines his 7 steps to finding your life purpose, which he uses as the back bone for his successful workshops that he delivers internationally. About Simone Vincenzi Growing Together Exponentially, known as GTeX, was founded in April 2013 by Simone Vincenzi and Ben Shorter, two passionate personal development coaches, who were inspired to create a community of empowered individuals, through the sharing of tools and techniques to rapidly increase results in all areas of your lives.

A lot of men struggle with body image or eating disorders which often fly under the radar. There are also people who have trouble finding their purpose in life. These are two things that Simone Vincenzi, Co-Founder of GTex knows about, and how to turn it around.   In this frank discussion, Simone highlights important areas and what was his own breaking point, and outlines his 7 steps to finding your life purpose, which he uses as the back bone for his successful workshops that he delivers internationally.   SHOW NOTES Are available to download directly from the website http://www.cjmswaby.com/podcast.html

Audio PodcastAired date: 1/26/2012 1:00:00 PM Eastern Time

Video Podcast (CC)Aired date: 1/26/2012 1:00:00 PM Eastern Time