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3 episodes with a2b
2 episodes with a2b
2 episodes with a2b
2 episodes with a2b
3 episodes with a2b
3 episodes with a2b
3 episodes with a2b
2 episodes with a2b
In episode 275 of The Just Checking In Podcast we checked in with Anthony Bedward. Anthony is a qualified person-centred psychotherapist and the Founder of a2btherapy. In this episode we chart his vast professional journey, from working as a mentor in a learning provision for young people expelled from school, to applying for a 10-week course at the Sherwood Psychotherapy Training Institute based at the University of Staffordshire and then completing a three year degree there afterwards. He then landed two part-time jobs, one working for a company that delivered Princes Trust courses to 16–25-year-olds and another as a youth worker. Anthony then found a new job, working with survivors of Childhood Sexual Abuse (CSA) at a charity called Imara Nottingham where they work with children and families to recover from the impact of sexual abuse on their lives. He worked there for eight months, before leaving to join the job he is in now and went onto set up A2BTherapy. We also discuss the lack of male therapists in the mental health space and why we need more men like Anthony, not just to help men, but working-class men and especially working-class black men like Anthony who will hopefully be able to put their trust in. We then explore his dyslexia diagnosis and how its shaped his life pre and post-diagnosis and the work he does with A2B. For Anthony's mental health journey, we discuss: the impact that being sexually abused as an adult had on his life, the importance of boundaries, figuring out his triggers and the levels of mental toughness you need to do the work he does. As always, #itsokaytovent You can find out more about a2btherapy here: https://a2btherapy.co.uk/ You can follow a2btherapy on social media below: Instagram: https://www.instagram.com/a2btherapy/ TikTok: https://www.tiktok.com/@a2btherapy Support Us: Patreon: www.patreon.com/venthelpuk GoFundMe: www.gofundme.com/f/help-vent-supp…ir-mental-health Merchandise: www.redbubble.com/people/VentUK/shop Music: @patawawa - Strange: www.youtube.com/watch?v=d70wfeJSEvk
Agile in Hardware: The Future of Agile Hardware Development, A Case Study From High Power Semiconductor Industry With Milad Maleki and Markus Thut In this Agile in Hardware episode, Milad Maleki and Markus Thut of Hitachi Energy Ltd. describe the pioneering methods and challenges of Agile hardware development in high-power semiconductor manufacturing. From introducing cutting-edge RoadPak technology for Formula E racing to optimizing consumer EV solutions, they share a fresh perspective on agile practices beyond the traditional software domain. Join us as we uncover the intricacies of an iterative approach to hardware design, production integration, and actionable insights for advancing Agile principles in complex hardware manufacturing. The RoadPak Project: Pioneering eMobility Innovation Milad and Markus kick off the discussion with the story behind the RoadPak project, a powerful technology supporting electric mobility and racing industries, including Formula E and Formula 1. Developed initially as a small-scale prototype, RoadPak has since evolved into a versatile solution with wide-reaching applications in consumer electric vehicles and fast-charging stations. “From racing to consumer EVs, RoadPak's journey showcases the transformative potential of scaling innovation from concept to consumer solutions.” Redefining Agile in Hardware: An Iterative Revolution Unlike traditional hardware development's linear approach, the team adopted an Agile model to adapt and evolve both the product and its manufacturing processes at the same time. By designing the production line in tandem with the product, they created a collaborative environment where feedback directly informed product design and production line adjustments. “In Agile hardware, the manufacturing process becomes part of the product itself—a continual feedback loop between design, production, and customer needs.” Iterative Sample Development: The A, B, C, and D Samples Milad and Markus discuss the use of progressive sample iterations (A, B, C, and D) to refine RoadPak's development. But, within each of the sample phases, they iterated quickly, for example having samples from A1, A2, A2b, etc. This approach provided invaluable insights, allowing for cost-effective tools and small-scale prototypes that rapidly incorporated feedback from customers and the production line. “Every iteration helped us with fast and impactful learning cycles which refined both product design and manufacturing. Proving that fast feedback is crucial—even in hardware.” Customer Feedback and Early Prototyping: Shortening the Feedback Loop To ensure RoadPak met real-world requirements, the team engaged customers early and often. They relied on simulation, rapid prototyping, and laser-cut parts to accelerate the feedback process. A specialized “evaluation kit” enabled customers to test the component in their own environments, exemplifying how quick delivery - even in hardware projects - can significantly speed up product development. “Early customer feedback is critical; our evaluation kit bridged the gap, turning theoretical design into practical functionality for real-world testing.” Integrated Development: Product and Manufacturing as Partners This episode emphasizes the unique challenges of developing the product and manufacturing process concurrently. By focusing on early quality control and optimizing the process on-site, they achieved higher yield and product reliability, setting the foundation for scalable, high-quality production. “For any new product, designing the manufacturing process alongside the product itself isn't optional—it's essential for quick feedback, and long-term success and quality.” Key Success Factors in Agile Hardware Development Markus and Milad highlight the importance of cross-functional teams, communication, and focus on dedicated resources. By streamlining their team's goals and processes, they maintained agility and clarity in the development cycle. This episode wraps up with tips and resources for those looking to apply Agile principles to hardware, emphasizing the value of flexible, collaborative workflows. “Focus and communication drive success in Agile hardware; with the right team alignment, you're equipped to adapt quickly and effectively.” Recommended Resources Milad and Markus suggest practical resources to deepen listeners' understanding of Agile in hardware. The book Scrum Essentials: Agile Software Development and Agile Project Management for Project Managers, Scrum Masters, Product Owners, and Stakeholders by Troy Dimes serves as an adaptable foundation. “Books and frameworks are starting points, but adapting Agile to hardware means integrating experimentation as a core part of the process.” About Markus Thut and Milad Maleki Markus Thut is a lead engineer at Hitachi Energy Ltd.'s semiconductor production in Lenzburg, Switzerland, specializing in high-power semiconductors and eMobility innovations. Markus is recognized for his forward-thinking approach to automation and industrial innovation, rooted in Swiss precision and a dedication to bringing visionary ideas to life. You can link with Markus Thut on LinkedIn and connect with Markus Thut on Twitter. Milad Maleki is the Head of R&D for high-power semiconductors at Hitachi Energy. With a PhD from École Polytechnique Fédérale de Lausanne (EPFL), Milad has led groundbreaking research and development initiatives in the semiconductor field, championing collaboration and innovation to power a sustainable energy future. You can link with Milad Maleki on LinkedIn and connect with Milad Maleki on Twitter.
Longtime president of the Wisconsin Swimming Coaches Association and the next president of NISCA, Kyle Bedalov wandered into the Swim Talk Zoom Room recently and led us through a vast wonderland of thoughts and ideas. See the timeline below for your own personal roadmap through this audio delight! Email us: swimtalk.a2b@gmail.com =========================== Timeline for this above-average episode: 00:00:01 – Disclaimer & banter 00:02:40 – How to be a Great Swim Parent 00:04:01 – Introducing Kyle Bedalov 00:05:01 – Kicking off a new season 00:07:06 – Swimming south of the border 00:08:17 – WISCA site 00:11:58 – Wisconsin high school poll 00:14:24 – Just when I thought I was out… 00:18:03 – Competing through Covid-19 00:21:50 – Private schools into WIA 00:23:07 – Coaching your own 00:29:10 – Swimming at the next level 00:35:11 – Swim lesson PSA 00:36:40 – Social Media 00:42:55 – A day in the life… 00:45:35 – More social media 00:48:17 – The Question Behind the Question 00:51:35 – One Shot Wednesday 00:53:15 – Chop Wood Carry Water 00:54:32 – Future of NISCA leadership 00:59:19 – NISCA convention plans 01:00:36 – Archive, records, & A-A fixes 01:02:37 – NISCA podcast w/Rowdy 01:04:05 – Virtual awards banquet 01:06:16 – A2B guest roster 01:07:33 – Wrapping it up =========================== Music Credits: [1] Hot Swing Kevin MacLeod (incompetech.com) Licensed under Creative Commons: By Attribution 3.0 License http://creativecommons.org/licenses/by/3.0/ [2] Private Eye Kevin MacLeod (incompetech.com) Licensed under Creative Commons: By Attribution 3.0 License http://creativecommons.org/licenses/by/3.0/
Panayiotis Panayiotou, also known as Panikos, was born in 1979 to parents Anastasia and Mihalis Panayiotou. Panikos began this whole journey into production with the publication Amathus to Birmingham (2016) inspired by his own parents who are also refugees from the 1974 Turkish invasion. ‘A2B' as this project is known, documented the stories of families who moved from Cyprus to Birmingham. During this project it became apparent that the female perspective needed more attention and he was inspired to search deeper into the stories of the strong females in the community. This then inspired him to create his 1st documentary Queens of Amathus, and in his own words ‘due to the sacrifices of the Queens of yesterday, is the reason we live the life we live today'. Panikos is now an established documentary maker currently working on a series of projects which include ‘The Queens of the Commonwealth' a celebration of migrant women from various backgrounds and their journey to the UK. He is also currently working on the sequel of Queens of Amathus re living the journey of Cypriot women from Cyprus to the rest of the world.Panikos also followed his father Lakis' dream and created Lakis Greek Kitchen where he shares, cares and supports people in need. LGK is a traditional Greek Taverna with a clay oven serving authentic Greek Cypriot cuisine. Annual pop up taverna and charitable organisation running a series of projects and fundraisers supporting the community.https://lgk.org.uk/productions/
ASX kicked another 41 points higher to a record close of 7812 (0.5%). Banks were solid again, CBA up 1.1% and ANZ slightly higher. The Big Bank Basket trading at $208.94(0.6%). MQG slid 0.8% despite stories of a bid for part of Heathrow Airport. Insurers flat. REITs rallied with GMG leading the way up 3.7% and MGR up 2.2%. Industrials too were solid, WOW up 0.2% and TCL running 1.1% higher. Tech stocks better, REA up 2.3% and XRO rallying 1.4%. The All-Tech Index up 1.0%. In resources, iron ore rallied dragging FMG up 3.5% on news that Twiggy is looking at copper, RIO up 0.9% and S32 continuing to rally up 1.7%. Gold miners mixed. EVN up 2.4% and SFR up 2.8% as copper stocks are in focus. Lithium stocks under a little pressure as Citi turned bearish. Oil and gas better, WDS up 1.2% and coal and uranium better. In corporate news, two more bids today, MRM and MEA and A2B voted in favour of the takeover and will be delisted in early April. Nothing on the economic front today. Asian markets were mixed, Japan down 0.6%, HK up 0.3% and China up 0.3%. 10-year yields back at 4% .Why not sign up for a free trial? Get access to expert market insights and manage your investments with confidence.Ready to invest in yourself? Join the Marcus Today community.
ASX 200 finished flat at 7502. Xmas apathy took over despite a rally on Wall Street. Inflation numbers tonight but not expecting much. Banks were flat with the Big Bank Basket down to $189.53(-0.1%). The $190 level has been resistance in the past. Financials also slipped. MQG down 0.5% with insurers drifting slightly lower, QBE off 0.4%. REITs powered ahead with GMG up 0.7% and SCG rising 0.3%. 10-year yields once again under some pressure at 4.03%. Chinese yields hit a 30-year low. Industrials sagged, TCL down 0.4%, WES off 0.3% and TLS falling 0.3%. Tech slightly better with NXT up 0.5% and WTC moving 0.9% higher. Old Skool Platform stocks tickled higher with REA up 0.5% and SEK doing well. Retailers mixed. Always so. KMD fell again, LOV up 0.4% with APE bouncing back 2.4%. In resources, CXO collapsed 21.2% on a strategic review as lithium prices have been trashed this year. PLS up 3.0% and LYC up 2.9% on China rare earth moves, iron ore miners still firm FMG up 1.1% on a new high. Gold miners flat and oil and gas better. Plenty going on in the M&A space, PBP up 15.5% on a bid, A2B also getting a bid up 19.9% and ASB jumped 7.2% on a new US Navy contract. MIL raced 73.4% ahead with a bid too. Nothing on the economic front. US inflation numbers tonight another piece in the jigsaw. Asian markets better with Japan up 0.2%, HK up 1.3% and China up 0.7%. Why not sign up for a free trial? Get access to expert insights and research and become a better investor.Make life simple. Invest with Marcus Today.
7月31日才新開幕的OMO3淺草，很多朋友有興趣。很快的來整理一下，以免我忘記。 OMO3 Asakusa by Hoshino Resorts（OMO3浅草 by 星野リゾート） 官網：https://hoshinoresorts.com/ja/hotels/omo3asakusa/ Agoda：https://reurl.cc/eDr8Dm 1.位於13樓的OMO Base對愛看風景的朋友，可以是入住這個旅館的一切理由。 一面是看淺草寺，一面是看東京晴空塔的絕佳位置！這裡24小時開放，有自助付費提供各種飲料和輕食的Food and drink Station。還可以更上一層樓到14樓的Rooftop Terrace，也是24小時開放的露天展望台！ 2.這旅館位置就在二天門外，走過去到淺草寺本堂差不多一分鐘！這應該就是他最大的優勢。我最推薦的時間是清晨和夜晚，沒有這麼多遊客的淺草寺。 3.交通方面可能就稍微離站遠一點，大概就是從雷門走到淺草寺本堂前的寶藏門那樣的距離。 東京metro銀座線淺草站7番出口 徒歩約4分 都營淺草線淺草站 A5番出口 徒歩約6分 東武淺草站北口 徒步約2分 筑波快線淺草站 徒歩約9分 最常利用的應該是銀座線，走回來的路上會遇到一蘭拉麵，星巴克，宇奈多多鰻魚飯，松屋。當然在淺草仲見世通東邊那一區域也有很多餐廳可選擇。 這裡是東武鐵道往日光、鬼怒川的起點，東武到晴空塔也只要一站喔。我最後一天早上是走Sumida River Walk散步過去，然後從東京晴空塔站坐車回來。 值得一提的是機場交通雖然從淺草線可以直通運轉羽田成田兩個機場，但帶著行李的話淺草線有電梯的出口A2b就離得比較遠了，大概要走個9分鐘吧。 4.和去年底我住的淺草六區相比較，淺草六區的生活機能明顯勝出，有驚安殿堂，有多個超市，交通也多了一個筑波快線可以利用。但當然從OMO3這裡也可以穿過淺草寺過去逛就是了。 5.房型方面，我這次訂的是最多間最小的房型やぐらルーム，15平米，下鋪是張小雙人床，上鋪是可以休憩的地方（和大塚的類似房型上下舖相反）。窗戶是沒有辦法打開的，沒有窗景，房內也沒有浴缸只有淋浴。 另外雙床房TWIN有18和33平米兩種，應該就都有窗景。以官網的圖看來前者可能是面向晴空塔，後者是面向淺草寺，且有浴缸。 6.這間旅館沒有餐廳，他的早餐就是在Food and drink Station吃，早餐時段會有提供飯糰，麵包等等。很多都是淺草當地的店家，另外也有看到OMO自己做的三明治。訂房有附餐券的方案（Food & Drink Station 1000円チケット付），據說可以省500yen。（大塚是OMO5，有自己的餐廳，早餐是現做滿精緻的） 7.既然是OMO，最大的特色是員工會帶你深入的認識當地。4PM在OMO base會簡單的介紹淺草的歷史還有美食文化，這不用報名即可參加。每逢星期五六晚上會有落語。最熱門的是每天早上5:55AM集合帶你去淺草寺散步的行程，大概一個小時，約7AM在雷門前解散。理論上說要在前一天10PM前報名，我當天是2點多check in的，然後就忙著照房間忘記這件事，猶豫了一下結果在3點報名就額滿了！因為他只有10個名額。建議想參加的朋友，入住的第一件事，一定要報名。不過我隔天早上還是厚臉皮的路過去旁聽了，哈哈！他全程是日文，懂日文的朋友，我很建議參加！ 8.館內著要另外付費（大塚也是這樣），不能借傘，只能用買的，1100yen。一樓有些簡單的OMO相關周邊，一樣也是可以自助結帳。 說個結論，光光她從13~14樓鳥瞰淺草寺這個窗景我覺得就值得來住一次了。但如果說週邊生活機能或是交通的方便性，可能離車站更近一些的選擇會更好喔。比方說我去年有住過的淺草東武，或是淺草六區的Richmond。 歡迎追蹤林氏璧孔醫師的發聲管道，了解最新的日本旅遊訊息！ 我的電子名片 https://lit.link/linshibi 日本優惠券大平台和近期活動資訊 https://linshibi.com/?p=20443 歡迎贊助04b喝咖啡 https://pay.firstory.me/user/linshibi
In today's podcast I talk about: My road trip from Palakkad to Bengaluru. Breakfast at A2B. Bengaluru traffic. Meeting Gaurav Rathore and his family. Late Dinner. Breathing to improve restorative sleep.
In conversation with the incredible running couple Sadhana and Sudhakar on their journey of conquering the Abbott world majors together. Sadhana is a management graduate who currently works with her family business in the well-known South Indian restaurant chain A2B. Sudhakar is an engineer and tech professional. They have two lovely kids. They are an everyday couple with a common passion for running and well-being, and they achieved this incredible feat together. Chapter timelines 0:00 Intro and background 7:20 The beginnings 12:47 Moving to the USA and signing up for the half marathon 17:34 The first full marathon 21:28 The start of Abbot's major journey 24:23 Running medals as jewelry 30:11 The feeling of shaving off 90 mins for PR 32:53 The next three majors 39:52 the decision to move back to India 42:12 Balancing parental responsibilities 44:23 Positive impact on marital relationship 49:04 Training in India 57:58 The Tokyo finale experience 1:08:40 What's next on the horizon 1:11:05 The fun Q&A round 1:20:16 The last word
Tea on Transfer is back! We asked the TN community what they thought about the term ‘transfer shock' and this month we're talking about it! In this episode, we will cover: the term's origin, how it has evolved, and how transfer professionals can best support students during their transitional period at both the sending and receiving institutions.About Our HostsDr. Heather Adams Ed.D. is the senior manager at The Aspen Institute College Excellence Program, leading the development of transfer engagement initiatives. She is also the founder and CEO of Transfer Nation. Dr. Adams earned an A.A. in Psychology from Santa Monica College and transferred to UCLA, where she obtained a B.A. in Psychology and an Ed.D. in Educational Leadership.Emily Kittrell is the assistant director at the National Institute for the Study of Transfer Students (NISTS). She previously held roles in testing, registration, and student orientation at a primarily transfer-sending, two-year campus. Emily earned a bachelor of science degree in Psychology from the University of Central Arkansas and a master of science degree in Family Studies from the University of Kentucky.Resources MentionedRead Inside Higher Ed's Beyond Transfer blog article on Transfer Shock: Myth or Reality by Kerstin Gentsch, Sarah Truelsch, Yoshiko Oka, and Alexandra W. LogueReview one of the first mentions of ‘transfer shock' in this 1965 paper by John R. Hills on The Academic Performance of the Junior College TransferRead about prior perspectives and processes surrounding Transfer Student Adjustment (Laanan, 2001)Check out the A2B team's NISTS Conference 2023 presentations on transfer reform and faculty engagement. Presentation slides will be available in the NISTS Conference Archives later this spring.Check out Inside Higher Ed's Higher Ed Gamma blogCheck out the Survival Guide to Transfer Shock by Diane Dominique Perez, UCSD, Class of 2020Review a research paper on transfer shock and graduation rates among engineering students (Smith et al., Journal of Engineering Education, 2021)#TeaOnTransfer #TransferTea #TransferResourcesLet us know what you want to see in the next Tea on Transfer episode by sharing your thoughts and suggestions on any of our social media!See y'all in Portland! And if not, tune in! Transfer Nation will be live at the conference, with updates, lessons learned, and special guests!Keep talking with Transfer Nation IG: @WeAreTransferNation TikTok: @TransferNation Twitter: @TransferPride FB Group: Transfer Nation Email: WeAreTransferNation@gmail.com Talk soon!#TransferPride #TransferSuccess #TransferChampion #TNTalks #TransferNation
ASX 200 fell 129 points to 6858. Off lows but caution remains. Losses across the board with the Big Bank Basket down to $186.24 (-1.6%). MQG down 1.4% with insurers holding up as 10-year yields rose to 3.91%. MPL up slightly. Fund mangers in focus as PPT rose 7.1% on a potential bid, PDL dropped 10.7% as its party could be spoilt and MFG just lost 5.7%. Healthcare down as CSL fell 1.6% dragging SHL lower and COH falling 3.5%. Industrials weaker, staples in focus on WOW numbers, COL fell1.0 %, WES down 3.8% and EDV off 1.4% with WOW falling 3.5% on quarterly results. TLSDA gained as a defensive up 1.3% but REITs sliding on rate woes. GMG down 0.8% with LLC down 8.7% on a strategy update. Tech on the nose again, the Index down 1.5%, XRO off 2.0% and SQ2 off 5.7% as US slumped. Resources fell with BHP down 3.2%, FMG off 2.9% and RIO down 2.2%. Lithium was a little depressed, PLS down 1.4% and MIN off 3.1%. Gold miners fell despite no change in bullion, NST off 3.4% and NCM down 3.0%. Energy stocks mixed, WDS fell 1.4% yet NHC rose 5.9% on new buy back. In corporate news, BVS was guillotined by a business update from the new CEO falling a massive 52.1%, A2B fell 7.4% on guidance reaffirmed, PPT up 7.1% on a bid at 3000c cash from Regal and its partner. A2M rose 4.2% on USA tick and JAN rose 17.0% on a trading update. In economic news, strong exports helped the trade surplus to $12.4bn. In Asia, Japan closed for a holiday. HK fell hard as it raised rates, falling nearly 3% and China down around 1.2%.Why not sign up for a free trial? Get access to expert insights and research and become a better investor.
It's the end of our first pre-week before we get into the real work next week. We discussed Habit 1, which is exploring how to remove the word SHOULD from our communication. Habit 2 is to feel not just think, about what you want, as though you already have it. Now, Habit 3 for today and the weekend, is remembering that "Everything Just Is". In other words we give everything meaning, so we can change the meaning of anything. It's called re-framing. If I ask you to think of a car, what do you see in your mind's eye? A2B, a polluter, status symbol, traffic jams, passion, a home? If I use the word Vaccination, which is polarising and highly emotive. Is the word a relief, a necessary evil, an invasion, or a cure? We will all interpret the word differently, according to our influences, beliefs, preferences and social conditioning. There's no right or wrong. It's just what it is because Everything Just Is.
Equine Search and Rescue is a thing.... Kate has been inspired by this training as we have been playing Hide & Seek with Rum. Because the horse can access fast and different terrain, follow a scent and the ability to detect movement far away it makes them perfect for the job. Yet again we are in awe of how amazing horses are. So what does that mean for us? Well Hide and Seek is a great recall game, it's similar to A2B but with a different slant as the horse has to find the human. We have been playing this game with Rum, hiding in different places and in different areas and we will progress with this. Why bother to play Hide and Seek? Why not? Its great fun and you really engage the seeking system in the horse, recall is always an advantage and if you ever needed your horse to find you..... viola the training is already there. If you are enjoying our podcast please share and subscribe. You can follow Kate and Becky on Facebook, Instagram and YouTube. Equilogical Positive Equine Partners and Little Green Stables. Don't forget to join us on Patreon: Little Green Stables for all our training videos and more!!!
After road-testing the latest T-Dance set for Pride, we thought we'd get ahead of the curve and do a late-week drop before the weekend. Pride T-Dance Edition 2021 is part of the Party Favorz re-entry into T-Dance, which was a staple in the early years of this podcast. This special Pride edition is chock-full of updated gay anthem classics as well as current House Music tracks that are perfect for Sunday afternoon reveling or frankly, anytime you want something a little less hard-hitting than our traditional circuit and anthems sets. Dropping this now will allow for the creation of Gay Anthems For a New Generation 2021 with a (hopeful) release by this upcoming Sunday but don't hold us to that. If we can clear the slate — it will buy us some time to start the Apple Music transfer that was discussed in the Pride Edition 2021 last week and is going to be a long tedious process. Additionally, we were informed that the issues surrounding our app had been resolved but they still persist. So, that's going to require additional testing, which in turn necessitates more free time. Ultimately, an earlier release of these sets allows folks already celebrating Pride before the official U.S. date and the international Pride Day a week later to get into the spirit as these events take place. This is another whopper of a set and we didn't hold back. There's more than enough here to fill up your little queer hearts. Until the next time...ENJOY! I want to give a special shoutout to Dirk S. ($50) and Michael H. ($100) for their unexpected yet very generous donations! You guys are much appreciated!!! Album: Pride T-Dance Edition 2021 Genre: Vocal House, Disco House, Gay Anthems Year: 2021 Total Time: 04:55:58 1. Gloria Gaynor - I Will Survive 2020 (Eric Kupper Remix) 2. Diana Ross & The Supremes - You Keep Me Hangin' On (Jet Boot Jack Remix) 3. Dirty Disco vs. Donna Summer - She Works Hard For The Money (Dirty Disco Classic Rework) 4. Dua Lipa - We're Good (Dirty Disco Mainroom Remix) 5. John LePage feat. Debby Holiday & LFB - Heaven (John LePage & LFB Original Club Mix) 6. Madonna - Vogue (Division 4 Extended Mix) 7. The Weather Girls - It's Raining Men (Barry Harris Refresh 2018 Mix) 8. A2B with Pumpkin Spice & Bugie - If You Could Read My Mind (Dirty Disco & Matt Consola Mainroom Remix) 9. Brett Oosterhaus & Kalia Medeiros - Love So Hard (Dirty Disco Mainroom House Remix) 10. Diana Ross - Love Hangover (Dirty Disco Classic Tribute Remix) 11. Freemasons feat. Jackie Moore - Love On My Mind (Jet Boot Jack Remix) 12. Disco Gurls feat. The Soul Gang - From Desire (Extended Mix) 13. Ralphi Rosario feat. Samantha Blanchard - You Used To Hold Me 2021 (Hifi Sean Backroom Mix) 14. Sean Finn & Selda feat. Inaya Day - Don't Cha (Extended Mix) 15. Heather Small feat. Dirty Disco & Matt Consola - Moving On Up (Division 4 & Matt Consola Remix) 16. Yaz - Don't Go (Jet Boot Jack Remix) 17. Nacho Chapado & Ivan Gomez - We Got The Look (Extended Mix) 18. Guy Scheiman & Inaya Day - High Energy (Lucius Lowe Classic Mix) 19. Ultra Nate - Free (Jet Boot Jack 2019 Remix) 20. Sylvester - You Make Me Feel (Mighty Real) (Ralphi Rosario Club Mix) 21. Thelma Houston - Don't Leave Me This Way (Jet Boot Jack Remix) 22. Manuel Grandi - Young Hearts Run Free (Original Mix) 23. Lissat & Jackers Revenge - Can't Take My Eyes Off You (Original Mix) 24. Dirty Disco vs. Erasure - A Little Respect (Dirty Disco Mainroom Remix) 25. Donna Summer - This Time I Know It's For Real (Block & Crown, Paul Parsons Remix) 26. The Shapeshifters feat. Billy Porter - Finally Ready (David Penn Extended Remix) 27. Cheryl Lynn - Got To Be Real (Alien Disco Sugar Remix) 28. Block & Crown feat. Culum Frea - Pull Up To My Bumper (Nudisko Club Mix) 29. Guy Scheiman - Disco Dance (Original Mix) 30. Pet Shop Boys - It's A Sin (Barry Harris 2021 Remix) 31. Cher - Gimme! Gimme! Gimme!
After road-testing the latest T-Dance set for Pride, we thought we'd get ahead of the curve and do a late-week drop before the weekend. Pride T-Dance Edition 2021 is part of the Party Favorz re-entry into T-Dance, which was a staple in the early years of this podcast. This special Pride edition is chock-full of updated gay anthem classics as well as current House Music tracks that are perfect for Sunday afternoon reveling or frankly, anytime you want something a little less hard-hitting than our traditional circuit and anthems sets. Dropping this now will allow for the creation of Gay Anthems For a New Generation 2021 with a (hopeful) release by this upcoming Sunday but don't hold us to that. If we can clear the slate — it will buy us some time to start the Apple Music transfer that was discussed in the Pride Edition 2021 last week and is going to be a long tedious process. Additionally, we were informed that the issues surrounding our app had been resolved but they still persist. So, that's going to require additional testing, which in turn necessitates more free time. Ultimately, an earlier release of these sets allows folks already celebrating Pride before the official U.S. date and the international Pride Day a week later to get into the spirit as these events take place. This is another whopper of a set and we didn't hold back. There's more than enough here to fill up your little queer hearts. Until the next time...ENJOY! I want to give a special shoutout to Dirk S. ($50) and Michael H. ($100) for their unexpected yet very generous donations! You guys are much appreciated!!! Album: Pride T-Dance Edition 2021 Genre: Vocal House, Disco House, Gay Anthems Year: 2021 Total Time: 04:55:58 1. Gloria Gaynor - I Will Survive 2020 (Eric Kupper Remix) 2. Diana Ross & The Supremes - You Keep Me Hangin' On (Jet Boot Jack Remix) 3. Dirty Disco vs. Donna Summer - She Works Hard For The Money (Dirty Disco Classic Rework) 4. Dua Lipa - We're Good (Dirty Disco Mainroom Remix) 5. John LePage feat. Debby Holiday & LFB - Heaven (John LePage & LFB Original Club Mix) 6. Madonna - Vogue (Division 4 Extended Mix) 7. The Weather Girls - It's Raining Men (Barry Harris Refresh 2018 Mix) 8. A2B with Pumpkin Spice & Bugie - If You Could Read My Mind (Dirty Disco & Matt Consola Mainroom Remix) 9. Brett Oosterhaus & Kalia Medeiros - Love So Hard (Dirty Disco Mainroom House Remix) 10. Diana Ross - Love Hangover (Dirty Disco Classic Tribute Remix) 11. Freemasons feat. Jackie Moore - Love On My Mind (Jet Boot Jack Remix) 12. Disco Gurls feat. The Soul Gang - From Desire (Extended Mix) 13. Ralphi Rosario feat. Samantha Blanchard - You Used To Hold Me 2021 (Hifi Sean Backroom Mix) 14. Sean Finn & Selda feat. Inaya Day - Don't Cha (Extended Mix) 15. Heather Small feat. Dirty Disco & Matt Consola - Moving On Up (Division 4 & Matt Consola Remix) 16. Yaz - Don't Go (Jet Boot Jack Remix) 17. Nacho Chapado & Ivan Gomez - We Got The Look (Extended Mix) 18. Guy Scheiman & Inaya Day - High Energy (Lucius Lowe Classic Mix) 19. Ultra Nate - Free (Jet Boot Jack 2019 Remix) 20. Sylvester - You Make Me Feel (Mighty Real) (Ralphi Rosario Club Mix) 21. Thelma Houston - Don't Leave Me This Way (Jet Boot Jack Remix) 22. Manuel Grandi - Young Hearts Run Free (Original Mix) 23. Lissat & Jackers Revenge - Can't Take My Eyes Off You (Original Mix) 24. Dirty Disco vs. Erasure - A Little Respect (Dirty Disco Mainroom Remix) 25. Donna Summer - This Time I Know It's For Real (Block & Crown, Paul Parsons Remix) 26. The Shapeshifters feat. Billy Porter - Finally Ready (David Penn Extended Remix) 27. Cheryl Lynn - Got To Be Real (Alien Disco Sugar Remix) 28. Block & Crown feat. Culum Frea - Pull Up To My Bumper (Nudisko Club Mix) 29. Guy Scheiman - Disco Dance (Original Mix) 30. Pet Shop Boys - It's A Sin (Barry Harris 2021 Remix) 31. Cher - Gimme! Gimme! Gimme!
Adyar Anandha Bhavan more popularly known as A2B, a brand that every Indian associates with sweets, snacks, savories and some mouth watering food that can be counted upon for consistent taste and quality. This is the story of how a family stuck together through difficult times, overcame several obstacles and achieved the ultimate success in food business. A story that talks about values, practical advise and much more ... Please note that this episode is in Tamil.
Zebra Muscles AWAY https://thefishingwire.com/revolutionary-dip-tank-stops-invasive-mussels/ Gobies destroy Musky https://ottawacitizen.com/news/local-news/st-lawrence-musky-population-threatened-by-invasive-gobies Perchfest https://www.orillia.com/events/details/perch-festival-482?calendarMonth=2021-04-01 https://youtu.be/X3SUVBKU2Nk https://www.lakesimcoeliving.com/news/hot-tips-for-the-2019-orillia-perch-festival.html https://youtu.be/BNQWfBCIlc0?t=452 Lure Visibility https://youtu.be/AAJjdA6b4Ts https://en.wikipedia.org/wiki/Vision_in_fish Lure selection https://midcurrent.com/flies/shining-a-light-on-uv-materials/ https://nvlpubs.nist.gov/nistpubs/jres/4/jresv4n2p189_A2b.pdf Catching Carp https://www.carpbase.co.uk/beginner-carp-fishing/ Sponsors: AquaVu: aquavu.com Epidemic Sound: https://www.epidemicsound.com/referral/vn24py/ Support the show (http://www.youtube.com/orilliafishing)
On this episode, Dutch and Gibbler discuss this week's hot button in the head shaker segment: "To Vaccine or Not To Vaccine" and in A2B news we ask "Where is the craziest place you have taken a shower?
Alex Opacic is a 6 foot 10 ex-professional basketballer who has played in many teams all round the world and also back here in Australia. Alex started Athlete2Business, A2B works with athlete-candidates of all levels – from entry level to executive to senior management – and places them across clients from various industries – technology, media, manufacturing, engineering, recruitment, retail, sports and more. Check out Alex's website for more info - https://athlete2business.com.au/ Follow Alex on: FaceBook - https://www.facebook.com/aopacic Insta - @ahtlete2business TIMESTAMPS: 0:00 - Into to Alex's story 7:39 - Alex's US College Div 1 Highlights 11:51 - US now vs pre-C0V19 D 12:40 - College Ball Life 14:40 - Professional Ball Life 17:50 - Monday nights @ Bankstown 19:20 - Basketball journeys & leadership 22:30 - Basketball in Australia now vs 10 years ago 25:00 - Athlete 2 Business 30:15 - Re-inventing yourself in life 36:40 - Top 3 Top 3 46:45 - Alex's asks me a question 46:45 - Khaled's story of growing up in Lebanon 51:15 - How to get in touch with Alex
Email us at: swimtalk@outlook.com In the initial episode of Swim Talk A2B, Dana Abbott and Bob Button (The A and the B in "A2B") discuss: getting programs restarted after the initial Covid shutdown, training options while pools shut down, Endless Pools, close encounter with alligator in pool (almost), pro athletes setting good or bad examples? And NCAA and Olympic disappointment, what's ahead? * * * * * * * * * * Disclaimer: The comments and views expressed by the hosts and guests on Swim Talk A2B are their own and do not necessarily reflect those of their employers, friends, family, close acquaintances, or anyone they may or may not know in the various Witness Protection Programs. Email: swimtalk@outlook.com * * * * * * * * * * Music credits: Welcome to the Show by Kevin MacLeod Link: https://incompetech.filmmusic.io/song/4614-welcome-to-the-show License: http://creativecommons.org/licenses/by/4.0/ Corncob by Kevin MacLeod Link: https://incompetech.filmmusic.io/song/3554-corncob License: http://creativecommons.org/licenses/by/4.0/
Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2020.10.02.324517v1?rss=1 Authors: Fried, N. T., Maxwell, C. R., Hoek, J. B., Elliott, M. B., Oshinsky, M. L. Abstract: Identifying the mechanism behind delayed ethanol-induced headache (DEIH), otherwise known as the hangover headache, may provide insight into the mechanisms behind common headache triggers. Acetate was previously shown to be the key ethanol metabolite behind DEIH in the recurrent inflammatory stimulation (IS) rat model of headache. The reversal of trigeminal sensitivity following ethanol exposure with caffeine previously suggested a role of adenosine in DEIH. To characterize this, behavioral analysis and measurement of brainstem adenosine and glutamate with microdialysis and HPLC was performed while pharmacologically manipulating adenosine signaling in the IS and Spontaneous Trigeminal Allodynia (STA) rat models of headache. Blocking adenosine A2A receptor activation with istradefylline or acetate transport into astrocytes with the monocarboxylate transporter competitive inhibitor, alpha-cyano-4-hydroxycinnamate (4-CIN), prevented acetate-induced trigeminal sensitivity. Blocking adenosine A1, A2B, and A3 receptor signaling did not prevent trigeminal sensitivity. Compared to control rats, IS rats had greater increases in extracellular adenosine and glutamate within the trigeminal nucleus caudalis (TNC) of the brainstem during local acetate perfusion. Blocking transport of acetate into astrocytes with 4-CIN prevented the increase in adenosine and glutamate. Blocking A2A receptor activation prevented the increase in extracellular glutamate, but not adenosine in the TNC. These data are the first to demonstrate the physiological consequence of acetate on adenosinergic systems within trigeminal pain by suggesting that acetate-induced trigeminal sensitivity in DEIH is mediated by adenosine A2A receptor activation which modulates extracellular glutamate levels in the TNC. Copy rights belong to original authors. Visit the link for more info
Open discussion on how NOT to be an idiot when it comes to your health 0:58 Diet sodas and why they don’t help you lose weight 2:54 Chemicals and Artificial Sweeteners 3:38 Zombie Eating 7:15 The Importance of Eating Together 9:15 How to Properly Wear a Mask During the Pandemic 12:50 Other Pandemic Idiot Proof Tips 14:33 “Executive Medicine Moment” At Executive Medicine of Texas we understand that knowledge is power. That’s why we assess each individual’s health and risk factors thoroughly before providing a road map to better health. Learn more about how you can take charge of your health at http://www.EMTexas.com 14:55 Diabetes – Don't Be an Idiot 18:33 Heart disease – Only Idiots Ignore Chest Pain 22:20 “Pressing Health”- Health news that’s fresh off the press. Researchers at the University of Bonn have discovered a receptor in mice that regulates both the size of our abdomen and the size of our muscles, two things that change as we age. It looks like this may also exist in humans. The study, which also involved researchers from Spain, Finland, Belgium, Denmark and the USA, was published in the journal Cell Metabolism. There’s an A2B receptors in brown adipose tissue. And you may remember from our previous shows that brown fat is the good fat, the kind we need. Tt turns out that the more A2B a mouse produces, the more heat it generates because these antennas increase brown fat activity, which burns the bad fat. Despite their increased fat burning, the animals weigh hardly less than mice with fewer receptors, but they appear slimmer because they have less overall fat and more lean muscle. If indeed this research holds true in humans it could mean a major breakthrough in prolonging our youth. https://www.sciencedaily.com/releases/2020/06/200625115916.htm 23:24 Idiot Proof Your Relationships – Healthy People Have Healthy Relationships Learn more at: Website: StayYoungAmerica.com Twitter: @StayYoungPod Facebook: @Stay Young America!
Simon Besteman mag in de keuken kijken van A2B internet, oprichter Erik Bais geeft hem inzicht in hoe het bedrijf zich heeft ontwikkeld. Ook spreken we over IPV4 en IPV6, en waarom we nog steeds niet klaar zijn voor de overstap, ook al zijn de IPV4 adressen nu helemaal op.
Naveen Munjal is the Managing Director of Hero Electric Vehicles Pvt. Ltd., a part of The Hero Eco Group. As an entrepreneur, Naveen’s leadership and vision are widely recognized in shaping the Indian Electric two-wheeler industry as well as his contributions to various forums in the electric vehicle sphere both in India and globally. He has built the Hero Electric brand into a leading manufacturer and a market leader in the electric vehicles 2-wheeler market in India. At an International level, Hero electric launched A2B, a premium brand of electric bicycles, which is widely considered one of the pioneers in the EV industry globally. In 2008, he was instrumental in founding the SMEV (Society of Manufacturers of Electric Vehicles), the only representative body of the Indian electric vehicle industry in India.
Why one man's meat may have killed millions!!! (Click here to learn more) On this episode, the A2B team returns to the studio to educate you all about the world's last pandemic, the Spanish Flu. Be sure to visit www.after2beers.com
A2B is a Google bus like system hoping to create an efficient transportation method for everyone commuting to work without affiliation to any one company. Click play to find out how! Guest: Harshita Arora, Founder A2B --- Support this podcast: https://anchor.fm/sparklinkpodcast/support
Entrepreneurs dream about starting their own business, but they can’t afford it. How can they reach their financial goals and objectives to fund and grow their business? Most of them borrow money from their friends, parents, and/or credit cards. Today, I am talking to Bruce Mack of Platinum Trust Group. Bruce is an avid real estate investor and licensed financial advisor. He shares seven options to fund your business and take it to the next level. You’ll Learn... [03:54] Option 1: Revolving Lines of Credit Program is easy to qualify for with 700+ FICO score and more than one open lines of credit; no business plan, collateral needed. [08:55] Option 2: Installment-based Lending Platform features 25 lenders offering $1,000 to $50,000 with lower FICO score, but provable income. [12:25] Option 3: Business Directed Retirement Account (BDRA) is rollable IRA or 401(k) where funds from previous employers are accessible for specific transactions. [18:28] Option 4: Transactional Funding for A2B, B2C transactions, such as funds for wholesale flips. [20:07] Option 5: Platinum Trust Group/Division offers bulletproof asset protection and ability to save passive income money to repurpose. [24:48] Option 6: Private and Hard Money Solutions with low annual percentage rates (APRs) and 1-2 points to cash out rental property income to deploy on new projects. [26:42] Option 7: Plug-and-Play Scenario is relationship-oriented opportunity to connect and network with partners and sponsors. [29:17] Where to start? Typically, it takes about $75,000 to get your business started. [32:56] Funding Mindset: If you don’t want to go into debt to do anything, it may hold you back from growing your business and generating revenue. [35:35] Constant Lawsuits: Property managers/management companies that aren’t real estate investors are in high-risk business. Tweetables Donuts to Dollars: Entrepreneurs start businesses thanks to friends, family, and credit cards. Plug-and-Play Option: You never know, who you know. Get your project going. You’re in the wrong business, if you don’t want to go into debt to grow your business and generate revenue. Protect your assets! Property managers/management companies that aren’t real estate investors face constant lawsuits. Resources Platinum Trust Group Platinum Financing Group FICO Fundbox IRA 401(k) Real Estate Investor Association (REIA) DoorGrowClub Facebook Group DoorGrowLive DoorGrow on YouTube DoorGrow Website Score Quiz Transcript Jason: Welcome to DoorGrow Hackers to the DoorGrow Show. If you are a property management entrepreneur that wants to add doors, make a difference, increase revenue, help others impact lives and you are interested in growing your business and life and you're open to doing things a bit differently, then you are a DoorGrow Hacker. DoorGrow Hackers love the opportunity, daily variety, unique challenges and freedom that property management brings. Many in real estate think you're crazy for doing it, you think they're crazy for not, because you realize that property management is the ultimate, high trust gateway to real estate deals, relationships and the residual income. At DoorGrow, we are on a mission to transform property management businesses and their owners. We want to transform the industry, eliminate the BS, build awareness, change perception, expand the market and help the best property management entrepreneurs win. I'm your host, property management growth expert, Jason Hull, the founder and CEO of DoorGrow. Now, let's get into the show. Today's guest, who I'm hanging out with, is Bruce Mack of Platinum Trust Group. Bruce, welcome to the show. Bruce: Thank you so much. I really appreciate it and you definitely are unbelievable at your opening. Jason: Thank you. It's built around all the challenges that we've heard in the industry and what our client-centric mission is as a company. I wanted to fold all that into our intro and I appreciate you giving us some positive feedback on it. Bruce, I'm really interested in getting into this. Today's topic is seven options to fund your business. This is a common challenge of people not being able to afford to do work with us, being able to afford to do the things they need to do to grow their business. This is a common challenge. There's a lot of entrepreneurs that are just trying to operate just paycheck to paycheck. In order to get ahead and grow the business, they need to find some funding, or get some money, or figure out how to make it work, or save something in order to make that work. Before we get into that, could you give everybody a bit of a background? Let's qualify you, help them understand how you got into what you're doing? Tell us, who is Bruce? Bruce: Well, in a couple of sentences or at least a short paragraph, I am an avid real estate investor, have been in a three-year period of time. I was able to buy rehab and flipped out over 160 properties. I've been involved with over $92 million worth of real estate transactions, SFR’s, as well as commercial. I'm a licensed financial advisor, prior owner and operator of a credit repair company that was also a licensed and bonded. I've been around the block. I love working with real estate investors. I speak to them all over the world, as well as nationally and have been at countless events helping folks just like the folks that are on this podcast, to be able to reach their financial goals and objectives, through getting them the rudimentary financing that they need so they can take their business to the next level. Jason: Okay, great. Let's get into the seven options. I guess we're starting with number one. Bruce: Okay, let's start with number one. One of our premiere programs that we use on a daily basis is what I call our revolving lines of credit program. Now, the nice thing is with this particular program, is that there are a lot of no's, but these are no’s that you want to hear, not no’s that you don't want to hear. Bruce: One of the no’s is that you don't have to have a business plan. Another no is that you don't have to have collateral pledged to be able to qualify for this, so if you don't have any collateral, i.e. properties, what have you, or other hard assets, there are no collateral pledges. Another no is that you don't have to have an income verification because it's a stated program. Without a business plan, without having a stated income, without having to go through a bunch of hoops, this makes it an easy qualify program. The key qualifiers are having a FICO score, ideally north of 700 or 700 when we put you through the program, and having more than one (ideally) open lines of credit with a credit card that would be at least $6000, $7000, $7500 worth of credit limit, and at the time that we put you through, you're ideally at 30% or lower on your utilization. Let's just say you have a $10,000 credit card. Let's say you have a $7000 balance currently, that would be at %70 utilization. What I'm saying is that we’d like to see that that $10,000 credit card has no more than %30 utilization or that you're not currently carrying more than a $30,000 balance. Now, if you are, because there's too much month, not enough money and therefore you have higher balances, we do have a solution. We do have another funding division that will likely take a look at those balances and work with you to actually pay them down for you, so that therefore your scores will skyrocket to where we need them to be, your utilization ratios will plummet to where we need them to be, so that we get the maximum results. The maximum results is our average client on a first round funding gets $75,000 worth of revolving lines of credit as much as $150,000 on a first round fund. When done properly and if we have a client that comes to us with longevity of accounts, no derogatory, so on and so forth, of course, that's going to get us all a better net result for the client on the back-end. Again these lines of credit are all at 0% APR for up to 21 months. Jason: Okay, but the cruz behind this is that they've got to have good credit in place in order to do this one. Bruce: Well, there's a couple of other if’s, and’s, and but’s, so let's talk about them briefly. Number one, because of that high utilization, we have taken people with scores as low as 620 and just by paying down those balances, they've shot their scores up within a several week period of time to well over 700 and then we can put them through. Today's present credit score may qualify you even if you're not knocking on the 700 door or higher, we need to do a consultation and see if the net effect of paying down those cards is going to get you to where we need you to be. Secondarily, we do have a secondary program, in as much as if the client can't qualify, but they have what we call a credit partner—maybe it's a partner in their business, maybe it's a relative, a friend—we can use a credit partner to get the same results, thus being able to put them through the program and that could be a win-win. There's a number of different ways we can literally skin the cat to get to the desired result, which is to get the client funded on that program. Jason: All right. You’re going to help them the pay down process, they can use a credit partner, there's a couple of options there. That's number one, the revolving lines of credit program. Bruce: Number two, one of our other core programs is we have 25 lenders. We have a platform for the 25 lenders and they are offering on the platform anywhere from $1000 to $50,000. We can stack those offers, so if you were to get two $50,000 offers, obviously you pony those up and parlay them into $100,000. Now, we can take more credit-challenged folks. We have gotten people some funding with FICO’s as low as 580. The key here is that there needs to be provable income, where the income on the revolving lines of credit is stated. These will need to be proved up through either showing the last couple of pay stubs and/or from their doing account validation by showing bank statements, 1099s, a year's tax return or what have you. Jason: Okay. Bruce: Very, very simple program, 12 questions asked and answered online, a soft pool with instantaneous pre-approvals and funding within usually a week or a week-and-a-half. This is a secondary program that can be used. We use it all the time and it's very, very effective. Jason: With these 25 lenders, these would be people like maybe Fundbox and some of the services out there. Would it be like those kinds of companies? Bruce: Could be, yes. We have our own lender pool that we work with. The nice thing is, there are a number of lenders that you can apply to on the net today, tomorrow, yesterday, what have you, but that's problematic. Every time you apply, you're going to be getting an inquiry. Every inquiry's going to be anywhere from two to several points and it starts to drag down your profile. Worse than that, other creditors that your applying with, see than you’ve been applying. The way we do it is when you access our platform with one soft inquiry, so it doesn't even show on your report, you're getting one or multiple preapprovals from multiple lenders at multiple options in terms of length anywhere from 12 months out to as long as 60 months or five years. This is an ideal way where you have no FICO hit, no negatives, only positives and you can get the pre approvals before you even press the accept button and go into what we call the final underwriting or the hard underwriting. Jason: Got it. Anything else to know about this second option? What would you call this second option? You're 25 lenders platform or? Bruce: Our 25 letters platform or our installment-based lending platform. Jason: Got it, installment-based. All right, so we’re on the number three now. Bruce: Number three. Let's talk about what we call our BDRA. Our BDRA stands for Business Directed Retirement Account. Now, many of the people that are on the podcast have a rollable IRA or 401(k). Maybe, they're even working and are aware that they have a roll-able IRA or 401(k) amount. Let's just say that you're currently working at an employer. You may have $100,000 there and your employers told you, “Well, you can't touch that, it's not rollable.” They’re may be half correct, because prior to coming to the existing employer, you worked at another employer. When you're at that other employer, guess what? You had a $50,000 IRA, which you’ve been rolled over to your present employer. Well, I'm here to tell you some really great news. You can do what's called a carve-out, so you can take those moneys and move them from your present employer, because those were moneys that came from a previous employer and you can automatically put them into what we call our BDRA. That BDRA is a wonderful opportunity for you to be able to access those funds to do what you want and what you want with them. Jason: That’s called what again? Bruce: Business Directed Retirement Account. Jason: Okay, got it. Bruce: Now, it's not a very, very different than a self-directed normal account. Some self-directed retirement accounts have the ability to give you checkbook capability, which is great. The BDRA coincidentally also does, but many of the self-directed accounts are accounts that once you moved on from your old employer, you've moved them into a self-directed environment so that you can tell your money what you want it to do. The problem with the traditional IRA or 401(k) in a self-directed environment (which many administrators that are out there and offer these types of accounts) is that you cannot use these but for very specific types of transactions. Let's just take a typical real estate transaction, a house costs $200,000, you have $100,000 in the self-directed retirement account. You need to come up with $100,000. Now, unfortunately, you cannot obligate a self-directed retirement account, a traditional type, not ours, but a traditional type and you cannot take on a recourse loan, because one of the exemptions is you cannot sign and obligate your IRA or your 401(k) to an external obligation. If you can't do that whole deal inside your IRA, you're pooched. You can't do the deal. Now, there is the possibility of taking on what's called a non recourse loan where you wouldn't sign. However, there are very few and far between. They never go more than 50% of LTV and they're usually a couple of points higher for all the right reasons. You’re only having a collateralized value of the loan. With a BDRA, I've got great news, you can take recourse loans on and it's not a violation of the BDRA precepts. Secondarily, when you have a normal IRA or 401(k), unfortunately, you're exempt from being able to do what we call inter familiar transactions because they're called a prohibited transaction. Meaning, father-sister, mother-brother, siblings what have you, you plain and simple are not allowed to invest with them because it's prohibited. That is not the case with the self-directed that we have in the BDRA environment. Third, you can put up to $53,000 of your annual salary into this tax deferred vehicle where you cannot with a traditional IRA or 401(k) that’s self-directed. Fourth, you can use the money for any business purpose. Now, you mentioned earlier that you've got coaching programs sometimes that are $10, $20, $30, $40, $50 whatever the amount is, it makes no difference, but the flexibility of the BDRA is a beautiful thing because BDRA funds can be used for any business purpose whatsoever. When you talk about a traditional IRA or 401(k), they're very finite, they're very linear, real estate being one of them, stocks and bonds being another, and there's a couple of others, and pretty much after that, you're out of luck. The flexibility that the BDRA brings to the table is phenomenal, and it is a great way to resource funds for enhancing your real estate business not only from the buying of the doors perspective, but from doing rehabs, for potentially using it for marketing money, to expand your net. There's many, many different ways that these moneys can be used that are all in conformity. Jason: Okay. Alright, BDRA is number three. Number four? Bruce: We have transactional funding. With our transactional funding, I'm sure a number of folks that are on this podcast are engaged with wholesale flips, where you're doing an A to B and B to C transaction. Well, we have transactional funding. We have $1 million on the sidelines at all time. You let us know, give us a couple of days notice. I mean, give us more notice than that, but within a couple of days, we can get the funds prepared, move them electronically to your escrow so that you can close and not have to be out of pocket if you're the wholesaler, and get the job done. The fee cost for that is the most reasonable that I've seen in the industry. The cost for funds is only 1.75% and a $495 transaction fee or our processing fee. Call it what you will. That’s another win-win strategy if you're a wholesaler, and you don't have the funds, and you're going to a traditional escrow. This is a perfect, perfect way to make everything come together so that you can get your property sold to that new buyer. Jason: Is that everything about transactional funding? Bruce: That's everything about transactional funding. Short and sweet. Jason: All right, let's look at number five. Bruce: Let's talk about number five. Now this is an esoteric way of getting funding, but saving the dollar obviously gives you $1 as I put it, redeploy or repurpose and I'm sure we all agree with that, and saving tens of thousands of dollars or more starts to become very, very interesting, let’s tell you how. On the other side of our business, we have our trust division. It's called platinum trust group. Platinum trust group is dedicated to bulletproof asset protection. I'll touch on that in a moment, but let me talk about the money aspect where you can redeploy. Real estate investors by the nature of who they are and what they're [...] are involved with two types in multiple streams of what we call passive income. The passive income that we're talking about would either be long or short term capital gains and/or lease and/or rental income. That is the sum and substance of what it's all about. One or the other. With our proprietary trust which we have 58 copyrights on, we've had the trust for over 20 years, we have over 31,000 clients on this program. As a real estate investor, when the properties are sold or the rents are collected, money goes into the corpus of the trust. However, the good news is, you can use the trust for any trust-related activities which would be anything other than what we call food, fun, or fashion. Now you're doing all your business out of the trust. Don't get me wrong. That doesn't mean you can't buy properties, you can't buy cars, that doesn't mean that you can't act in a fiduciary capacity as a trustee to do everything you would normally be doing on a daily basis. The good news is, that moneys, that long- and short-term capital gains which could be 20%, 30% depending, and/or the income from the lease and rental income, the fact that it goes into corpus and stays in the corpus, and that it’s deferred in perpetuity means you're not going to have the tax bill at the end of the year. Now, we have many investors who have tax bills in the $50,000, $100,000, $200,000 a year and are paying quarterlies that are enough to choke a horse. We're able to defer up to 97% of that annual tax liability, including the quarterlies, and deferred out in perpetuity, which means in 21 years, after the last of the beneficiary heir’s deceased, i.e., 100, 200, 300 years from now, we now have a vehicle that nobody in your family tree is going to have the tax consequence and certainly not you, and now we've got all of this additional liquidity that we can be using for investment purposes and is a huge win for our real estate investors. That's only one piece of the coin because the other side of that coin is the bulletproof asset protection, because you can never have a lien or judgment executed against you. It can't happen, let alone your properties because your properties are in the titanium vault of the trust. This is huge and this is a great, great income opportunity and/or savings opportunity for you. I think we're at number six or are we in number five? Jason: We’re at number six. Bruce: All right. Jason: That was number five. Basically. we will call that your trust division. Bruce: Okay, number six. We have a number of private money solutions and hard money solutions. Solutions that start as low as 4.9% on the APR and 1-2 points. Solutions for clients who have rental income properties and they want to do some cash out. We had even a blanket loan program which is available in 43 states. Again, if you've got properties, we have a solution for you to be able to access a ton of money that you are currently not able to access so that you can redeploy it on new projects. This could be huge for you by our hard end or private money funding. Should you have ground-up projects that you're looking to get underway, these are other ways that we can access funds for you depending upon what the project looks like. There's just so many different machinations without knowing more. We would really need to sit and talk, but not only can we get you the blanket loans, not only can we get you the cash out refis, we can do multifamily, we can do SFRs, ground-up projects. It just depends on what it is that you're looking for. Jason: Okay, great and that's number six. Bruce: That’s number six. Jason: Private and hard money lending solutions. Let's get into number seven. Bruce: Number seven is really a relationship-oriented proposition. Because I lecture on a nationwide basis and know so many people, I am constantly sourcing and/or resourcing and putting folks together. I speak. I meet sponsors. Sponsors are always looking for people to act as general partners for with other people who are newer and/or what I would call green peas and vice-versa. I have green peas that are looking for sponsors. Just by nature, the fact that I love to network, love to help people out, and if people are looking for a connection, I'll give you an example. Yesterday, I had a guy come to me in the Seattle area. He is looking to do a conversion. He’s got 93 apartments that he wants to build in one structure. He’s looking for general partners and money partners and he's got everything ready to go. He’s got the water. He’s got the utilities. He’s got all the zoning. He’s looking for money partners and he's also looking for some management help. Well, we have the perfect fit for him because we have people who are right up in the Seattle area because I've spoken recently up in the Seattle area to 800 people at one event. That’s an easy plug-and-play scenario. Oftentimes, you just never know. I don't know where you're calling in from on a nationwide basis because I know you have callers all over the place. I'm California-centric but I travel. I was just in Boston speaking to 1000 people. You never know who you know and tell me about the situation, and if there's a possibility that we can help, we certainly can try and plug to good ends into one another so that you can make a whole and get your project going, so you can take it to the next level. Jason: Perfect. Looking at all these different methods, let’s say I get a client that comes to me and they want to hire staff. They want us folks on marketing, maybe they want to do some coaching stuff with us, they need to get office space, these typical things to get their business going. Which channel would you push them towards first? What would be the best situation for them first? Bruce: Well, if the need is an average of, say, $75,000 roughly, somewhere between $50,000-$150,000. I’ll kind of use that $100,000 spread. invariably, our revolving line of credit program is the sweet spot and we utilize that at promoter events all the time for that $20,000, that $30,000, $40,000 to get them off the home plate, to get them the coaching program that they need to get them also the initial marketing moneys that they need so that they can really start to get traction and move forward in the marketplace. It's very easy and they don't need to have any collateral. Again, it's a state of program. If the person fits the parameters, it's by far and away, the easiest, fastest, most effective, and cost-effective solution. Jason: Now, what if they just wanted something smaller? They're just getting started, they're bootstrapping. Maybe they're looking for maybe $3000-$20,000, something in there. They just need to get some additional funds to get some things going in the business. Would the recommendation still be the same vehicle? Bruce: Depending. Let's just say today the need is $3000-$20,000. Let's just say they've got $100,000 locked up at the old employer that they used to work for, General Dynamics, let's just say. They're taking that money and they're turning it in the stock market, they're getting a horrible return, and they want to take control of it. I would move all of that to self-directed environment and then parse out where you've got total control over it. Then, I would parse out whatever that amount is that you need to deploy for whatever business purpose. If they only needed $3000, $7000, or $10,000 of that $100,000, they get immediately deployed because they have total discretionary use over the funds once it's in their dominion. Likewise, another one of our programs might be for them to engage with the 25 lender platform. In a request, only request $3000 or only request $10,000, if that's what it is they're looking for. That could be another way to go. We really need to have a discussion. It's my best suggestion to the folks that are listening because sometimes during the course of discussion, we find a $3000, $7000, or $10,000, may not actually be the sum and substance of what you're looking for depending upon where you are, and where you want to go. Maybe it is. We will come up with based upon your credit what you bring to the table, what's going to be the most cost effective way to get you there. Jason: Let's address the mindset of funding. I'm sure there's people listening and they want to bootstrap everything. They're thinking, "I don't want to go into debt to do anything." What would you say to that? Maybe that mindset is holding them back from being able to grow their businesses quickly and generate more revenue as fast. Bruce: I don't mean to be pragmatic but I would say they might be thinking about being in the wrong business if they don't want to go into debt. I bought houses utilizing credit cards before. If you go to any REIA, anywhere in the United States—if you're not familiar with the term REIA, that's Real Estate Investors Association meeting—if you go to any Real Estate Investors Association meeting anywhere in the United States and you interview, take them out for coffee, talk to them after the meeting, what have you, you ask them how do they get the funds to buy some of their first properties, I can guarantee you, dollars to donuts, that they borrowed money from a friend, borrowed money from their parents, or borrowed money from their credit cards, to get their first property. Or a combination of all three coupled together to make it happen. They didn't have the money and their checking account. It was a little devoid or little depleted at that time. Guys, this is truly a leverage play, and an arbitrage play, when you're borrowing money at X because you can make lie times X equally that new number which is the ability to compound on the amount of money that you're using to be able to get you that much bigger amount of money at the backend. I'm a firm believer in making the right decisions and not getting these moneys for a C shed or man cave. Forget it, you don't [...] it. If that's your ultimate goal, that's not leverage. That's just sheer stupidity, a waste of time, and a waste of money. If you're looking to get these moneys to be able to deploy them in an efficacious way and to utilize them to gain the leverage to be able to get a much bigger payday down the road when you exercise your exit strategy, let's go. Let's make it happen. We're here to help and get you to your financial goal. Jason: Plant some of these things. I know there's some property managers listening that are like, "I'm not a real estate investor." Some property managers that are running property management companies are not real estate investors. I think many of them are involved but they're thinking, "What about my business? Maybe I need funding for the business." I think the same principle applies. The idea that I want to point out is mindset-wise, I think a business is probably one of the most effective (if you do this well) investments you can invest in a period. Very few things give a return on an investment that a business can. I don't think even real estate, I think a lot of things cannot yield as high of a return as a business that is profitable, and highly effective. If the investment is moving the business towards those things, I would imagine that it's going to far outplay a 401(k) or any other sort of investment. They might be throwing them dollars towards in the long run. An effective business can yield a huge return especially once they sell it. Or it can just be an ATM machine feeding them once they systemize the business and they step out of being involved in it. If you're going to that, I think it's wise to say, make sure it's going towards the right thing. It's going to yield the ROI you're looking to get. Bruce: May I ask a brief question? Jason: Go ahead. Bruce: About your audience. I just heard or maybe I misheard, I heard you keying in on property management, and property management companies. Is there a broad segment of your listenership that are in that space? Jason: Yes. Most of the listeners listening are people that run property management companies. They manage properties for and on behalf of investors. Bruce: Okay. Let me just say this about that. I'm going to go back to, I think, it was number six. It might've been number five but it was right in there. We talked and drilled down a little bit about our proprietary trust. Guys, I'm going to say it just like it is, you are in an uber high risk business. Property managers and property management companies, they play it simple, they get sued. Facts are one in three Americans get sued. Two in three, 66% of all surgeons get sued. Property managers, I don't know what the numbers are, but everytime I talk to a property management company, they're constantly getting sued. Just recently, we put on several property management companies who have gotten the trust. Their prime motivating reason was to have the trust be the owner of the property management company so that they would not have liens or judgments that could be affixed to the company. Guys, this is something you definitely want to explore further. It's very important for you because of the high risk nature of the business that you're in. Jason: Yeah. I agree. I have an asset protection attorney. I think it's a wise choice for everybody who has some asset protection struff going on with things in the trust and make sure the business is protected. Very cool. We've got several people that I've spoken to even recently. They're like, "I don't have the funds to work with, Jason, but I want to work with you. We're trying to get money." Or they're trying to get their business started. Or they know there's some things they need to do and they can't just afford to do it. How can they get in touch with you? How can they reach out? What's the best way to connect with you and what you've got going on? Bruce: If you're looking for funding, I'm going to give you a web address. That web address would be platinumfinancinggroup.com. There's a calendar on there. We will get you a complimentary consult. Please, we'll ask you, make sure that you've mentioned that you came from Jason. We always want to know where clients came from. Jason: Mention DoorGrow and the DoorGrow Show. Bruce: Please. Please, please, please. That'll get you the complimentary consultation now for financing. When Jason's got great programs which I've heard nothing but fabulous things about, that can be the genesis, give you the capital to be able to move your business forward, and get his programs. Secondarily, another way to access the programs, as I've said, from the savings from the tax deferral, from the trust program, and/or talking about the trust as well as an asset protection vehicle. Because if you get wiped out, you're done. You know that. This is one way to ensure that you're not going to get wiped out. I would go to platinumtrustgroup.com. We have another calendar there. The difference between the two, other than the information that you're going to find and the calendars that you're going to find is that the calendar times that you're going to get blocked out for are quite different. If you go to platinumtrustgroup.com, we're going to block you out for an hour. We can talk about trust. Likewise, we can also talk about funding should you have an interest in both. If you strictly go to platinumfinancinggroup.com, you'll be directed to a calendar for 15 minute blockout. Just be aware of that. When you make the choices to where's the best entry point to get in touch with us. Jason: If they're really looking at everything and they want to get the full kit, the best place to probably to go the platinumtrustgroup.com. You can also help them with the financing side of things as well. Bruce: Absolutely. Jason: Perfect. Bruce, it's been fabulous having you on the show. Thanks for taking us through all the different options. I wasn't aware that there were so many different options for funding. I appreciate all the info that you're able to share with us today. Bruce: I certainly appreciate you're allowing me to come on your show. It's been a pleasure. I look forward to chatting with you guys. We'll get you taken care of. We'll get you the funding so that you can take your business to the next level and protected as well at the same time. Jason: Fantastic. One thing I just thought off. A lot of our listeners run property management companies. They're all connected to investors. Do you have a sort of program or a relationship that you can make with these entrepreneurs that are working and dealing with lots of investors trying to get them into multiple properties and new properties? Bruce: Absolutely. Not only that, we need to talk because we have an affiliate program. Give me a call, let's have that discussion. That's a whole other discussion and another income stream, potentially, for you. I'm glad you mentioned that, Jason. That was a great heads up. Jason: Perfect. Bruce, it's great to connect and I will let you go. Bruce: Thank you so much for the opportunity again. Have a great day, have a great weekend. Jason: If you're a property management entrepreneur, who wants to grow your business, who wants to add doors, you're looking, you're feeling a little bit stuck, you're dealing with some of the typical challenges, you're trying to do SEO, pay-per-click, content marketing, and social media marketing, you're just not getting the ROI, you're not adding the doors you're wanting to. There might be something different. There might be some things that you're missing. You might have some leaks in your business that you can't see. Reach out to DoorGrow. We'll help you shore those leaks up. We'll help you get on a trajectory of growth. I will be honored to be able to coach you through that stuff. We can certainly help you redesign your website. If you need to go and test your shiny new website or your old website, go to doorgrow.com/quiz. See if it's got some leaks there. You could be losing tens or hundreds of thousand dollars in future ROI every month depending on how many leads or deals you are missing out on because your website isn’t upgraded. I want you to have an A+. Talk to DoorGrow and let's see if we can help you get that taken care of. Until next time, everybody. To our mutual growth. Bye everyone. You just listened to the DoorGrow Show. We are building a community of the savviest property management entrepreneurs on the planet, in the DoorGrow Club. Join your fellow DoorGrow hackers at doorgrowclub.com. Listen, everyone is doing the same stuff. SEO, PPC, pay-per-lead, content, social, direct mail, and they still struggle to grow. At DoorGrow, we solve your biggest challenge getting deals and growing your business. Find out more at doorgrow.com. Find any show notes or links from today’s episode on our blog at doorgrow.com. To get notified of future events and news, subscribe to our newsletter at doorgrow.com/subscribe. Until next time, take what you learn and start DoorGrow hacking your business and your life.
Trump to the hospital. New music from SDC Digital.
I've been up since 4:00 this morning trying to get the Halloween Edition 2019 knocked-out for you folks. I had been afraid there wasn't enough music to fill the set only to find out I had more than I could use. So much so, that I had to leave several great tracks on the cutting room floor because I didn't have time to go back and try to make them work. Regardless, I still threw the entire kitchen sink at this and have NOT had the time to preview it yet. I'll do that later. If you find any errors — let me know and I'll fix them. I still plan on having the next Dance Club set out by Tuesday at the latest. I have about 4 hours of MRIs tomorrow so I may have to pull an all-day marathon again to get it out in time. I wanted both sets out for this weekend but time has been an issue. As soon as this is posted — I'm heading to bed as I'm exhausted. I'll be back in a couple of days. Until then...ENJOY! Album: Halloween Edition 2019 Genre: Circuit, Tribal, House Year: 2019 Total Time: 03:09:46 1. Dirty Disco vs. Whitney Houston - I'm Every Woman (Dirty Disco Eagle Houston Remix) 2. Ralphi Rosario vs. The 202 Machine Reboot - Get Up (Rock Your Body) (202 Reboot Mix) 3. Ariana Grande & Social House - Boyfriend (Dirty Disco Mainroom Remix) 4. Dirty Disco vs. Katy Perry - Small Talk (Dirty Disco Private Remix) 5. Céline Dion - Imperfections (DJ FUri DRUMS Honest House Extended Club Remix) 6. Shawn Mendes & Camila Cabello - Senorita (Danny Verde Guaracha Remix) 7. TRAIG - Listen To The Music (Dirty Disco Mainroom Remix) 8. Tim Curry - Sweet Transvestite (Joel Dickinson Mix) 9. Ariana Grande, Miley Cyrus & Lana Del Rey - Don't Call Me Angel (Roberto Ferrari Club Mix) 10. Veronica Vega feat. Quavo - A Million (Alex Acosta Fierce Remix) 11. Todrick Hall - Wig (Sagi Kariv Remix) 12. Ashley O (aka Miley Cyrus) - On A Roll (Dario Xavier Brazilian Remix) 13. Big Kid feat. Audrey Callahan - Every Breath You Take (Andre Grossi & Leanh Remix) 14. Selena Gomez - Lose You To Love Me (Dario Xavier Club Mix) 15. Madonna & Swae Lee - Crave (Dirty Disco Mainroom Remix) 16. Tones And I - Dance Monkey (Dario Xavier Club Mix) 17. Kalendr feat. Laura Bryna - Sweet Revenge (Twisted Dee & Diego Fernandez Extended Remix) 18. Tony Moran & Erick Ibiza feat. Everett Bradley - Everyday People (Club Mix) 19. Grapefruit Sound Lab & Amuka - Don't Fall For it (Dirty Disco Mainroom Remix) 20. Cher vs. Chic - I Want Your Love & Understanding (Joel Dickinson Mirroball Remix) 21. Sagi Kariv feat. Daniel Yafe - So Many Men (Original Mix) 22. Micky Friedmann feat. Sophia Omarji - Flame Is Burning (Original Mix) 23. Alicia Keys feat. Miguel - Show Me Love (Guy Scheiman Remix) 24. Ralphi Rosario feat. Aneeta Beat - Looking Cunt (Jackinsky Big Room Mix) 25. Céline Dion - Flying On My Own (Toy Armada & DJ Grind Remix) 26. Dirty Disco feat. Brenda Reed - In The Name Of Love (Dirty Disco Mainroom Remix) 27. Jaguar Grace - To All The Boys (Girls) (Dirty Disco Mainroom Remix) 28. Macau feat. Nikki Valentine - Feel It (Junior Senna Remix) 29. Todrick Hall - Fag (DJ Furi Drums Snap House Club Remix) 30. Sam Smith - How Do You Sleep (Danny Mart Remix) 31. Lewis Capaldi - Someone You Loved (Danny Verde & Leo Blanco Remix) 32. Vassy - Trouble (Leo Blanco Club Remix) 33. A2B with Pumpkin Spice & Bugie - If You Could Read My Mind (Dirty Disco & Matt Consola Mainroom Remix) 34. Kian Blume - Stop The Show (Joe Gauthreaux Club Remix)
I've been up since 4:00 this morning trying to get the Halloween Edition 2019 knocked-out for you folks. I had been afraid there wasn't enough music to fill the set only to find out I had more than I could use. So much so, that I had to leave several great tracks on the cutting room floor because I didn't have time to go back and try to make them work. Regardless, I still threw the entire kitchen sink at this and have NOT had the time to preview it yet. I'll do that later. If you find any errors — let me know and I'll fix them. I still plan on having the next Dance Club set out by Tuesday at the latest. I have about 4 hours of MRIs tomorrow so I may have to pull an all-day marathon again to get it out in time. I wanted both sets out for this weekend but time has been an issue. As soon as this is posted — I'm heading to bed as I'm exhausted. I'll be back in a couple of days. Until then...ENJOY! Album: Halloween Edition 2019 Genre: Circuit, Tribal, House Year: 2019 Total Time: 03:09:46 1. Dirty Disco vs. Whitney Houston - I'm Every Woman (Dirty Disco Eagle Houston Remix) 2. Ralphi Rosario vs. The 202 Machine Reboot - Get Up (Rock Your Body) (202 Reboot Mix) 3. Ariana Grande & Social House - Boyfriend (Dirty Disco Mainroom Remix) 4. Dirty Disco vs. Katy Perry - Small Talk (Dirty Disco Private Remix) 5. Céline Dion - Imperfections (DJ FUri DRUMS Honest House Extended Club Remix) 6. Shawn Mendes & Camila Cabello - Senorita (Danny Verde Guaracha Remix) 7. TRAIG - Listen To The Music (Dirty Disco Mainroom Remix) 8. Tim Curry - Sweet Transvestite (Joel Dickinson Mix) 9. Ariana Grande, Miley Cyrus & Lana Del Rey - Don't Call Me Angel (Roberto Ferrari Club Mix) 10. Veronica Vega feat. Quavo - A Million (Alex Acosta Fierce Remix) 11. Todrick Hall - Wig (Sagi Kariv Remix) 12. Ashley O (aka Miley Cyrus) - On A Roll (Dario Xavier Brazilian Remix) 13. Big Kid feat. Audrey Callahan - Every Breath You Take (Andre Grossi & Leanh Remix) 14. Selena Gomez - Lose You To Love Me (Dario Xavier Club Mix) 15. Madonna & Swae Lee - Crave (Dirty Disco Mainroom Remix) 16. Tones And I - Dance Monkey (Dario Xavier Club Mix) 17. Kalendr feat. Laura Bryna - Sweet Revenge (Twisted Dee & Diego Fernandez Extended Remix) 18. Tony Moran & Erick Ibiza feat. Everett Bradley - Everyday People (Club Mix) 19. Grapefruit Sound Lab & Amuka - Don't Fall For it (Dirty Disco Mainroom Remix) 20. Cher vs. Chic - I Want Your Love & Understanding (Joel Dickinson Mirroball Remix) 21. Sagi Kariv feat. Daniel Yafe - So Many Men (Original Mix) 22. Micky Friedmann feat. Sophia Omarji - Flame Is Burning (Original Mix) 23. Alicia Keys feat. Miguel - Show Me Love (Guy Scheiman Remix) 24. Ralphi Rosario feat. Aneeta Beat - Looking Cunt (Jackinsky Big Room Mix) 25. Céline Dion - Flying On My Own (Toy Armada & DJ Grind Remix) 26. Dirty Disco feat. Brenda Reed - In The Name Of Love (Dirty Disco Mainroom Remix) 27. Jaguar Grace - To All The Boys (Girls) (Dirty Disco Mainroom Remix) 28. Macau feat. Nikki Valentine - Feel It (Junior Senna Remix) 29. Todrick Hall - Fag (DJ Furi Drums Snap House Club Remix) 30. Sam Smith - How Do You Sleep (Danny Mart Remix) 31. Lewis Capaldi - Someone You Loved (Danny Verde & Leo Blanco Remix) 32. Vassy - Trouble (Leo Blanco Club Remix) 33. A2B with Pumpkin Spice & Bugie - If You Could Read My Mind (Dirty Disco & Matt Consola Mainroom Remix) 34. Kian Blume - Stop The Show (Joe Gauthreaux Club Remix)
Swishcraft Radio Episode #381 1. Katy Perry - Never Really Over (Rip City Boys Remix) 2. Sia & Flo Rida - Wild Ones (Division 4 Remix) 3. Inaya Day, Venus - When Im Alone (The Cube Guys Remix) 4. Kygo & Whitney Houston - Higher Love (Dirty Disco Pure Love House Remix) 5. Debbie Gibson - Only In My Dreams (The Scene Kings Remix) 6. Conjure - Face The Music (Kaskade) 7. Antoinette Roberson & Dirty Disco - Sense Of Danger (Rip City Boys Club Mix) 8. A2B with Pumpkin Spice & Bugie - If You Could Read My Mind (Dirty Disco & Matt Consola Mainroom Remix) 9. Todrick Hall - Nails Hair Hips Heels (Jose Spinnin Cortes Remix) 10. Billy Porter- Love Yourself (Ralphi Rosario Ballroom Brawl Remix) 11. Yazoo - Don't Go (Rob Moore Remix - Consola Edit) 12. DJ Dan Murphy ft Kristen Pearson - Ready To Go (Consola's Edit)
On this episode, the A2B gang welcome guest host Jeremy Scarrette. We discuss old school cartoons and interview comedian Brad Williams. All that .... and that's really it.
High plasma aldosterone is an independent risk factor for cardiac mortality, but what is known about the underlying mechanisms linking high levels of aldosterone to cardiac ischemic events? Listen as Consulting Editor Donal O’Leary (Wayne State University) interviews lead author Shawn Bender (University of Missouri & Truman VA) and expert Judy Muller-Delp (Florida State University) about the work led by motivated undergraduate Maloree Khan, which tested the hypothesis that increased plasma aldosterone impairs adenosine-mediated coronary vasodilation. Bender and co-authors found that high plasma aldosterone levels impaired adenosine A2A receptor-mediated dilation, but not adenosine A2B receptor-mediated vasodilation. Using a dose of aldosterone that does not significantly elevate blood pressure, Khan et al observed aldosterone-mediated changes in coronary dilation to adenosine via downregulation of calcium-activated potassium channels. Listen as these experts discuss the role of aldosterone and the mineralocorticoid receptor in ion channel expression in the vasculature, as well as future next steps related to sex differences and cell-specific knockout models. Maloree Khan, Alex I. Meuth, Scott M. Brown, Bysani Chandrasekar, Douglas K. Bowles, and Shawn B. Bender Aldosterone impairs coronary adenosine-mediated vasodilation via reduced functional expression of Ca2+-activated K+ channels Am J Physiol Heart Circ Physiol, published June 14, 2019. DOI: doi.org/10.1152/ajpheart.00081.2019
Swishcraft Radio Episode #379 1. Heather Small ft. Dirty Disco & Matt Consola - Proud (Wayne G & Porl Young Remix) 2. Avicci ft Aloe Blacc - SOS (The Scene Kings Remix) 3. Martin Ikin Feat. Hayley May - How I Feel (Original Mix) 4. Temmora ft Karfma - Fire (Scotty Boy & Luca Debonaire Club Remix) 5. Jess Glynne & Jax Jones - One Touch (Todd Edwards Remix) 6. Bleona- Monster (Block & Crown - Consola Re-Edit) 7. Kygo & Whitney Houston - Higher Love (Dirty Disco Pure Love House Remix) 8. Madonna - I Rise (Dirty Disco Mainroom Remix) 9. Eddie Chacon - Rumors or Lies (Deanne Remix) 10. Laura Branigan - Gloria (Anthony May Remix) 11. Billy Porter- Love Yourself (Ralphi Rosario Ballroom Brawl Remix) 12. A2B with Pumpkin Spice & Bugie - If You Could Read My Mind (Dirty Disco & Matt Consola Mainroom Remix)
Featuring Dap Daniel, Griffinstein, Erase-E, Wet-Money and Luci Dream, Unykue The Kid, Moses Brown, Nicky Miles, Justs & Olga Palushina, Doddi, Una Stef, Kontac, Bobby Mardis, Mistah FAB, Do Or Die, San Quinn and Nonchalant, Doug Cash , Will Adams, Dennis Soloman King, Vashoun Stjon, Epi III, Littmus Steampunk Band, MNTY, A2B and Bipin Panthi.
With a massive heat dome sitting over 2/3 of the country this weekend, I can't think of a better way to cool down than with the annual Summer Edition featuring the hottest circuit beats! Typically, I split these massive sets up but decided to just let it run. Clocking in at almost 4 hours, this set is a combination of the latest club hits currently making the rounds or soon will be on the circuit. It also has of a bevy of last-minute Pride anthems from the gay pride events of June. This year, Pride dragged on a week longer than usual due to the World Pride taking place in New York. Nevertheless, I'm always astounded at how many DJs wait until the last minute to drop their latest entries — especially since the biggest event kicks off in late May in L.A. It's neither here nor there but it would be helpful if these tracks were released sooner than not to reach a wider audience. Regardless, Party Favorz is thrilled to have access to these tracks as most of our Circuit Tribal House sets are pretty much Pride events in and of themselves. The classic reworks, the bigger than life DIVA vocals, songs of love, empowerment, and heartbreak are always mainstays in the gay community. No need to go over everything here because the artist roster speaks for itself. I'll be starting on something I've been wanting to work on for a while — which is a big vocal house set (also geared to our gay friends) who enjoy a more housed-up, disco-fueled Sunday afternoon set. There's not a lot that comes out these days but the type of music I'm talking about is starting to make a slow comeback when the remixes get served to DJs. I really think you folks are gonna enjoy this one since we haven't had a good ole fashion T-Dance set in a very long time. I have to say that I'm pretty worn out and after this next set I'll be taking a week off from the site before Madonna's birthday month sets start rolling out. I'm way behind in that department so it remains to be seen if I'm gonna be able to pull off what I initially had planned. Never fear, whatever I end up doing — all you Queen of Pop freaks are gonna love it. That's all I got for now. I hope everyone stays hydrated and enjoys their weekend. Until the next time...ENJOY! Album: Summer Edition 2019 Genre: Circuit, Tribal, House Year: 2019 Total Time: 03:44:37 1. Sigala & Becky Hill - Wish You Well (Roberto Ferrari Intro) 2. Laura Branigan - Gloria (Anthony May 2019 Pride Mix) 3. Shawn Mendez feat. Camila Cabello - Señorita (Pavblo Ibarra & Dark Boy Remix) 4. Madonna - I Rise (Roberto Ferrari Remix) 5. Celine Dion - Flying On My Own (M. Torrez Remix) 6. Ed Sheeran & Justin Bieber - I Don't Care (Leo Blanco Remix) 7. Narcotic Thrust feat. Yvonne John Lewis - Safe From Harm (Alexander 2019 Tribute Mix) 8. Temmora feat. Karma - Fire (Mauro Mozart Club Remix) 9. Kylie Minogue - New York City (Toy Armada & DJ Grind 2019 World Pride NYC Remix) 10. Duncan Laurence - Arcade (VMC Epic Remix) 11. Elexis Ansley - Light Shower (Tony Moran Extended Remix) 12. Yinon Yahel & DJ Head - The One (Dirty Disco Mainroom Remix) 13. Elton John - Rocket Man (Barry Harris 2019 Remix) 14. Todrick Hall - Nails Hair Hips Heels (Barry Harris Club Mix) 15. Ariana Castelli - Love Is Love (Dan Thomas Tribal Circuit Remix) 16. DJ D-Sol feat. Alex Newell - Rescue Me (Ralphi Rosario Big Mix) 17. Rod Carrillo & Terri B - You Got What I Need (Dan Thomas Alfie Is Up All Night Mix) 18. Marc Stout feat. Jessica Sutta - Our Song Comes On (Rosario & Ibiza Club Remix) 19. David Guetta feat. Kelly Rowland - When Love Takes Over (Isak Salazar vs. Mauro Mozart 2019 Mix) 20. Jennifer Lopez feat. French Montana - Medicine (Dirty Disco Mainroom Remix) 21. Alfie Arcuri - Same (Dan Slater Remix) 22. Toy Armada & DJ Grind feat. Brian Kent - Just Say Yes (Original Mix) 23. A2B with Pumpkin Spice & Bugie - Read My Mind (Dirty Disco & Matt Consola Mainroom Remix) 24.
With a massive heat dome sitting over 2/3 of the country this weekend, I can't think of a better way to cool down than with the annual Summer Edition featuring the hottest circuit beats! Typically, I split these massive sets up but decided to just let it run. Clocking in at almost 4 hours, this set is a combination of the latest club hits currently making the rounds or soon will be on the circuit. It also has of a bevy of last-minute Pride anthems from the gay pride events of June. This year, Pride dragged on a week longer than usual due to the World Pride taking place in New York. Nevertheless, I'm always astounded at how many DJs wait until the last minute to drop their latest entries — especially since the biggest event kicks off in late May in L.A. It's neither here nor there but it would be helpful if these tracks were released sooner than not to reach a wider audience. Regardless, Party Favorz is thrilled to have access to these tracks as most of our Circuit Tribal House sets are pretty much Pride events in and of themselves. The classic reworks, the bigger than life DIVA vocals, songs of love, empowerment, and heartbreak are always mainstays in the gay community. No need to go over everything here because the artist roster speaks for itself. I'll be starting on something I've been wanting to work on for a while — which is a big vocal house set (also geared to our gay friends) who enjoy a more housed-up, disco-fueled Sunday afternoon set. There's not a lot that comes out these days but the type of music I'm talking about is starting to make a slow comeback when the remixes get served to DJs. I really think you folks are gonna enjoy this one since we haven't had a good ole fashion T-Dance set in a very long time. I have to say that I'm pretty worn out and after this next set I'll be taking a week off from the site before Madonna's birthday month sets start rolling out. I'm way behind in that department so it remains to be seen if I'm gonna be able to pull off what I initially had planned. Never fear, whatever I end up doing — all you Queen of Pop freaks are gonna love it. That's all I got for now. I hope everyone stays hydrated and enjoys their weekend. Until the next time...ENJOY! Album: Summer Edition 2019 Genre: Circuit, Tribal, House Year: 2019 Total Time: 03:44:37 1. Sigala & Becky Hill - Wish You Well (Roberto Ferrari Intro) 2. Laura Branigan - Gloria (Anthony May 2019 Pride Mix) 3. Shawn Mendez feat. Camila Cabello - Señorita (Pavblo Ibarra & Dark Boy Remix) 4. Madonna - I Rise (Roberto Ferrari Remix) 5. Celine Dion - Flying On My Own (M. Torrez Remix) 6. Ed Sheeran & Justin Bieber - I Don't Care (Leo Blanco Remix) 7. Narcotic Thrust feat. Yvonne John Lewis - Safe From Harm (Alexander 2019 Tribute Mix) 8. Temmora feat. Karma - Fire (Mauro Mozart Club Remix) 9. Kylie Minogue - New York City (Toy Armada & DJ Grind 2019 World Pride NYC Remix) 10. Duncan Laurence - Arcade (VMC Epic Remix) 11. Elexis Ansley - Light Shower (Tony Moran Extended Remix) 12. Yinon Yahel & DJ Head - The One (Dirty Disco Mainroom Remix) 13. Elton John - Rocket Man (Barry Harris 2019 Remix) 14. Todrick Hall - Nails Hair Hips Heels (Barry Harris Club Mix) 15. Ariana Castelli - Love Is Love (Dan Thomas Tribal Circuit Remix) 16. DJ D-Sol feat. Alex Newell - Rescue Me (Ralphi Rosario Big Mix) 17. Rod Carrillo & Terri B - You Got What I Need (Dan Thomas Alfie Is Up All Night Mix) 18. Marc Stout feat. Jessica Sutta - Our Song Comes On (Rosario & Ibiza Club Remix) 19. David Guetta feat. Kelly Rowland - When Love Takes Over (Isak Salazar vs. Mauro Mozart 2019 Mix) 20. Jennifer Lopez feat. French Montana - Medicine (Dirty Disco Mainroom Remix) 21. Alfie Arcuri - Same (Dan Slater Remix) 22. Toy Armada & DJ Grind feat. Brian Kent - Just Say Yes (Original Mix) 23. A2B with Pumpkin Spice & Bugie - Read My Mind (Dirty Disco & Matt Consola Mainroom Remix) 24.
This week on EveryDay with Denise you’ll hear from Janet Coffman. Janet’s obedience lead her to found A2B Street Ministry in Tulsa. We think you’re going to love this story and hope that you take the opportunity to get involved with A2B! Text A2BGive to 44321 Give Online International Mission Board 1 Corinthians 13 Isaiah … Continue reading Episode 7: A2B Street Ministry
On this week’s show, Bree and Justin agree that two is the best number in the world, especially when it comes to video games! It's a hodge-podge of topics, including Guild Wars 2's latest episode, The Crew 2's launch, Trion Worlds' Gazillion acquisition, and more! It’s the Massively OP Podcast, an action-packed hour of news, tales, opinions, and gamer emails! And remember, if you’d like to send in your own letter to the show, use the “Tips” button in the top-right corner of the site to do so. Show notes: Intro Adventures in MMOs: World of Warcraft, Dungeons and Dragons Online, Staxel, Project Gorgon, The Sims 4, Trove News: Guild Wars 2 says "Long live the Lich!" News: The Crew 2 nears launch News: Elder Scrolls Online ticks off its fanbase News: Black Desert partners with an exploiter News: Trion Worlds buys up Gazillion assets News: Fractured seeks $116.5K in Kickstarter campaign Mailbag: What Trion Worlds should do with the Marvel stuff Outro Other info: Podcast theme: "A2B" from The Crew Your show hosts: Justin and Bree Listen to Massively OP Podcast on iTunes, Stitcher, Player FM, TuneIn, Google Play, iHeartRadio, Pocket Casts, and Spotify Follow Massively Overpowered: Website, Twitter, Facebook, Google Plus, Twitch If you’re having problems seeing or using the web player, please check your flashblock or scriptblock setting.
My guest on the show today is one of the original gangstas of the digital music business: Larry Miller. Half a decade before Apple launched iTunes, Larry was carving a path. In 1998, he co-founded a2b music, one of the first digital music distribution companies. He went on to become President of Reciprocal Music, another groundbreaking digitial distribution & technology company, and later co-founded the Or Music label, which won a Grammy with their artist Los Lonely Boys. These days, Larry is the Director of the NYU Steinhardt Music Business Program, where he’s shaping the intellect of young people who will write the next chapter in the history of the music business. There’s a very wise saying: History is written by the victors. Here’s the accepted history of the digital music business: The major labels were clueless about the internet and were doing nothing until Napster appeared and killed the music business. Luckily, Steve Jobs and Apple saved the day by inventing digital downloads and selling them in iTunes. True veterans of the early digital music business know that things were a bit more complex than that. It was an exciting time and things evolved very quickly, too fast to keep up with. The truth is that starting in the mid-90’s, many technology pioneers were working closely with the major labels to shape the digital future of the music business. It was an incredible time of experimentation and learning. Of course the ideas weren’t all viable, so the labels cautiously dabbled, thinking they’d control the timetable of how these new technologies would roll out. After all, that’s how it always worked whenever a new format came along. Of course this time they were wrong. Napster came along and burned all the cautious optimism to the ground. Steve Jobs had great timing, arriving when the majors were fatigued from fighting and desperate for an easy fix to complex problems. So a new history was written, and the story of what came before was brushed away like ashes. We did this podcast over an early breakfast at the Mansion Diner at 86th & York Avenue, hitting record and chatting amongst the sounds of New Yorkers waking up to a gorgeous Fall morning. Larry Miller on Wikipedia: https://en.wikipedia.org/wiki/Larry_S._Miller NYU Steinhardt: http://steinhardt.nyu.edu/music/business Musonomics Podcast: https://itunes.apple.com/us/podcast/musonomics/id985799104?mt=2 The Mansion Restaurant: http://www.mansionrestaurantnewyork.com Shawn Colvin & Steve Earle: http://www.rollingstone.com/country/features/steve-earle-and-shawn-colvin-talk-new-album-rehab-and-trump-w431117
Welcome Back! Bada is back and in the full swing of things in this Season 3 PREMIERE episode of A2B! Pokemon, Vapes, BLM and Gays ! NEW FORMAT NEW THEME SONG SAME BADA !!
Club Session Radio Show #2 Winky Wiryawan - mellow weather (Evan Virgan remix) Morgan Page - Safe Till Tomorrow (Pegboard Nerds Remix) Sam Feldt - Shadows Of Love Alesso - I Wanna Know Kungs vs Cookin' On 3 Burners - This Girl (Clean) AlunaGeorge - I'm In Control - Throttle Remix Madison Avenue - Don't Call Me Baby - DAAB Remix Matroda - Deep Inside TLC - No Scrubs (evan mashup) Big Von - Off Top (Dirty) J - Know - tipsy [ x - mix urban 70 ] J-lo feat Fabolous - get right [ dj A2B funky mix ] Snoop Dogg ft Pharrell - Drop It Like Its Hot Fat Joe & Remy Ma - All The Way Up - Tall Boys All Day Bootleg (Clean) Ludacris - Stand Up (evan mashup) The Chainsmokers - Don't Let Me Down - W&W Remix Sak Noel & Salvi - Trumpets (evan mashup) Ricky West & Dondersteen - Twerkout (Dirty) O.T. Genasis - Cut It - YDG Trap Remix Flo Rida - Low - Chunky Dip Drop That Low (When I Dip) Bootleg Beyonce - Formation - Artistic Raw Bootleg D.O.D - Taking You Back (evan mashup) Fais - Hey - EPX Sweet Harmony (evan mashup) G-Eazy & Bebe Rexha - Me, Myself & I - EPX Trigga Finga Bootleg Kaaze - Tell Me (evan mashup) Malaa - Notorious - Crankdat Remix Matisse & Sadko - Get Busy (Dirty) Max Vangeli & Flatdisk - Blow This Club MATTN & Futuristic Polar Bears - Cafe Del Mar 2016 (evan mashup) The Chainsmokers - Roses - Tony Arzadon Remix (Dirty) W&W - How Many Dannic - Light The Sky Lush & Simon & Paris & Simo - NY 2 LA - VIP Mix Coldplay - adventure of lifetime (evan mashup) Evan Virgan ft Elle Vee - Silence (Radio Edit) Want to be featured in Club Session Radio? Send your demo to: promoclubsession@gmail.com Download this episode for free here: smarturl.it/clubsession1 more info: Mixcloud ► www.mixcloud.com/evanvirgan evanvirgan.com instagram.com/evanvirgan youtube.com/evanvirganTV @evanvirgan twitter.com/evanvirgan facebook.com/evanvirgan
We're exploring whom to let go from your friend list, how to do it properly, and afterwards how to move on with your life. Having good friends is important, and just as important is your ability to acknowledge and identify the people to distance yourself. Not everyone you meet are suppose to be in your life. Some people you meet may last a lifetime while others a few weeks. In order to spend your precious time on Earth wisely, you have to know who to keep and when to cut friendly bate. Ashley explains her A2B way for identifying whom, why, and how to distance yourself from non-working, overplayed, and painful relationships.Featuring Keenan Cobb, Bill Barnett, and Ashley Berges
Только Лучший & Hастоящий Electro House! 1. A2B ft. Fara - Tell Me Where You Are (Godlike Music Port Remix) 2. Johan K ft. Lisia - Together (Electro Mix) 3. Chemical Brothers - Hey Boy Hey Girl (Dj Amor Remix) 4. Romadi - Sister (ANDI VAX Remix) 5. Rockit ft. Maury - Heroes (Original Mix) 6. DJ Smash & Yoko & Antoine - Volna (DJ RATEK Re-Work Mix) 7. Kirsty - Hands High (Afrojack Remix) 8. Christophe Fontana Ft. Jonny Rose - Feeling (Overex Remix) 9. Akustikrausch - housefrau (die hoerer remix) 10. SJ & Paul Jacobson - I Love You Stop (Last 3 Digits Remix) 11. Riggers vs Bass Invaderz ft. MC Cyanide - Beer, Whiskey & Marijuana (Original Mix) 12. DGYGZR ft. Arama Mara - LaLa Pistolera (Riggers Pistol Whipped Remix) 13. Chris Quirk - Dirty Funk (Original Mix) 14. David Guetta - The World is Mine (Positive Djs Remix) 15. Klaas - Pulsar (Original Mix) 16. Andy Jay Powell - Children Of Paradise (Club Mix)
1. A2B ft. Fara - Tell Me Where You Are (Godlike Music Port Remix) 2. Lodjica, Misha Gamma - On The Move (Misha Gamma Remix) 3. Eric Chase - Allein Allein (Jansen Remix) 4. DHP feat Sunday Girl - Not Alone (Mephisto Instrumental Mix) 5. De-Grees - I Believe (Dany Wild mix) 6. Chemical Brothers - Hey Boy Hey Girl (Dj Amor Remix) 7. Discomaenner feat. Crazy-Z - Follow Me (Pete Sunset Remix) 8. Gabry Ponte feat. Darius & Finlay - Tattaratta (Club Edit) 9. Jansen Ft. Percy Duke - Staring At The Sun (Michael Mind Remix) 10. Alexandr Krupsky - Prototype (Original Mix) 11. Kirsty - Hands High (Afrojack Remix) 12. Akustikrausch - housefrau (die hoerer remix) 13. Ferry Corsten feat. Aruna - Live Forever (Original Extended) 14. Armin van Buuren feat Fiora - Breathe In Deep (The Blizzard Remix)
WMC 2012 is here and in my back yard! It still is a bit surreal that I live in this extremely unique city known as Miami. With the Winter Music Conference around it's a great time to bring up the energy level for this special WMC edition of the Solcast. Lots of heavy funky and driving house music in this one, including tracks and remixes by J Paul Getto, DJ Dan, Sasha, Alex Kenji, M.A.N.D.Y. & more. I'll be dj'ing the A2B pool party at the Marseilles Hotel 3/22, and Metro Beat at Sushi Samba 3/24. Hope you dig the mix... PAZ!
Episode 127 widmet sich ganz der Bike&Trimm Messe in Salzburg. Mit den Highlights von A2B, Corratec, Giant, GT, Haibike, Scott, Shimano und Simplon. Zu gewinnen gibt es einen Cycloc Radwandhalter.
Fakultät für Chemie und Pharmazie - Digitale Hochschulschriften der LMU - Teil 01/06
Adenosin-Rezeptoren (AdoR) zeigen als Komponenten des Adenosin-Signalweges komplexe Interaktionen untereinander, sowie mit weiteren Komponenten, wie der Ecto-5´-Nukleotidase (CD73) und der Adenosin-Desaminase (ADA). Diese Rezeptoren sind zudem mit den verschiedensten Signaltransduktionswegen verschaltet. Innerhalb dieser Arbeit sollten mögliche Mechanismen gemeinsamer potentieller Regulationen dieser Komponenten anhand von eukaryontischen Modellsystemen, wie auch anhand einer klinischen Studie zur chronisch lymphatischen Leukämie (CLL) untersucht werden. Für die Untersuchung transkriptioneller Regulationen wurde eine semiquantitative RT-PCR mit Hilfe eines heterologen Standards für alle 4 AdoR-Subtypen (A1, A2a, A2b, A3) etabliert. Die Spezifität dieser Systeme konnte mittels Restriktionsverdaus sowie Untersuchung von Transfektanten in AdoR-freien Zelllinien („Chinese Hamster ovary“ CHO) nachgewiesen werden. Densitometrische Auswertung der Amplifikate ermöglichte eine gute Quantifizierung der untersuchten mRNS-Niveaus, die noch Unterschiede in den subtypspezifischen cDNS-Niveaus von weniger als 40% deutlich auflöste. Für Untersuchungen in eukaryontischen Modellsystemen wurden C-terminale EGFPFusionskonstrukte aller 4 AdoR in pEGFP-N1 (Clontech) etabliert und in CHO- bzw. HeLa-Zelllinien transfiziert. Erfolgreicher Nachweis der Etablierung der Konstrukte erfolgte über Sequenzierung der neusynthetisierten Adaptersequenzen und über spezifische Restriktionsverdaus. Desweiteren konnte über die membranorientierte Fluoreszenzverteilung der Fusionskonstrukte in den Transfektanten auf eine erfolgreiche posttranslationale Prozessierung und Transport des Konstruktes in die Zellmembran zurückgeschlossen werden. RT-PCR-Analyse von RNS-Präparationen dieser Transfektanten zeigte eine erfolgreiche Transkription der Konstrukte. Innerhalb der HeLa-Zelllinie konnten alle 4 Subtypen erfolgreich exprimiert werden, wobei jedoch inhibitorische AdoR (A1AdoR; A3AdoR) leichter zu transfizieren waren als die excitatorischen A2aAdoR/A2bAdoR-Konstrukte. Letztere Transfektanten zeigten deutlich höhere Tendenzen zum Absterben, was mit einem erhöhtem cAMPNiveau dieser Zellen zusammenhängen könnte. Physiologische Aktivität des A3AdoR-Konstruktes konnte durch Verminderung der Teilungsrate der Transfektante beobachtet werden. Innerhalb der einzelnen Transfektanten konnten keine großen Änderungen der mRNS-Niveaus des entsprechenden transfizierten Subtypen festgestellt werden, was mit einer „Downregulation“ des nativen Subtypes erklärbar sein könnte. Jedoch zeigten sich zum Teil deutliche Niveau-Änderungen in den jeweils anderen Subtypen der Transfektante, was auf eine Interaktion der AdoR-Subtypen auf der Transkriptionsebene hindeutet. Verschiedene Wechselbeziehungen könnten mit - zum Teil von physiologischen Interaktionen her- bekannten Beziehungen der AdoR in verschiedenen Zelltypen in Zusammenhang gebracht werden. Induktion der Transfektanten mit AdoR-Agonisten, wie 5`-N-Ethyl-Carboxamido- Adenosin (NECA) oder (R)-N6-(1-Methyl-2-phenylethyl)-Adenosin (R-PIA) ergab einen Anstieg aller AdoR-cDNS-Niveaus im Falle der Aktivierung der Adenylylcyclase (A2a/A2bAdoR), bzw. eine Abnahme bei deren Inhibierung (A1/A3AdoR), die auch zum Teil subtypabhängige Modulationen und deutliche Abweichungen zur Kontrolllinie (EGFP-HeLa) zeigte. Behandlung der Zellen mit Alkohol als apoptosisstimulierendes Signal führte zu einer starken Erhöhung aller Subtyp-Niveaus, wobei besonders die A2aAdoR- und die A3AdoR-Transfektante die größten apoptotischen Tendenzen und auch die größten Modulationen der Subtyp-Niveaus zeigten. Transfektion der Konstrukte in die CHO-Zelllinie ergab gut exprimierende Klone der A1AdoR- und A3AdoR-Transfektante, die über membranorientierte Fluoreszenz des EGFP-Anhanges, wie auch über Nachweis der mRNS nachgewiesen werden konnten. Im Falle der excitatorischen AdoR erhielt man nur schwach exprimierende Klone der A2aAdoR-Transfektante, die deutliche Tendenzen zum Absterben zeigte. Wiederum fand man bei der A3AdoR-Transfektante einen erheblich verlangsamten Zellzyklus, was auf einer zellzyklusmodulierenden Wirkung des A3AdoR in verschiedenen Zelltypen beruht und somit auf eine erfolgreiche Signalfortleitung in der Transfektante hindeutet. Induktion der A1AdoR-Transfektante führte zu einer zeitabhängigen Konzentrierung und Internalisierung der Fluoreszenz, was auf eine intakte Regulation und Desensibilisierung dieses Subtypes innerhalb dieses Klones hindeutet. Die B-lymphoblastoide Zelllinie Raji wurde als Modellsystem für AdoR-Signalwege innerhalb des lymphatischen Systems untersucht. Eine starke Präsenz des A2aAdoR konnte mittels RT-PCR nachgewiesen werden, dessen Stimulation mit dem AdoR-Agonisten NECA wiederum zu einem Anstieg der mRNS-Niveaus aller AdoR-Subtypen führte. Behandlung mit Alkohol führte zu einem größeren Anteil an absterbenden Zellen sowie zu einem leichten Anstieg aller AdoR-Subtypen und deutet wiederum auf eine Beteiligung der AdoR an apoptotischen Antworten hin. Inwiefern substratmodulierende Komponenten des AdoR-Signalweges, insbesondere CD73 und ADA, in Regulationsmechanismen dieses Weges integriert sind, wurde in einem induzierbaren B-lymphoblastoiden Modellsystem, der 493-6-Zelllinie untersucht. Hierbei wurden durch induzierbare transkriptionelle Aktivierung zweier Mitogene, c-myc und EBNA2, vier unterschiedliche proliferative Zustände erzeugt und die Auswirkungen auf die Komponenten des Ado-Signalweges untersucht. Northern Blot-Analyse der 4 Zustände zeigten eine Zunahme der CD73- und A2aAdoR-mRNS-Niveaus mit sukzessiver Abschaltung der Mitogene, wobei EBNA2- Abschaltung den deutlichsten Effekt zeigte. ADA-mRNS-Niveaus zeigten meistens eine leichte Abnahme mit Abschaltung der Mitogene, was aber nicht eindeutig bestätigt werden konnte. Untersuchung der Zustände mit semiquantitativer RT-PCR ergaben eine Präsenz aller AdoR mit einer starken Überrepräsentierung des A2aAdoR. Wiederum konnte mit sukzessiver Abschaltung der Mitogene ein deutlicher Anstieg des A2aAdoR beobachtet werden, der wiederum deutlicher bei Abschaltung von EBNA2 ausfiel. Andere AdoR-Subtypen zeigten jedoch so gut wie keine Respons. Abschaltung der Mitogene führte sowohl bei EBNA2, wie auch bei cmyc nach ca. 3 bis 6 Stunden zu einem deutlichen Anstieg der A2aAdoR-Niveaus, sowie generell zu einer Abnahme des ADA-Niveaus im Falle der c-myc-Abschaltung. NECA-abhängige Erhöhung des cAMP-Niveaus korrelierte mit den A2aAdoRNiveaus der entsprechenden Proben. Ebenso konnte in diesem Labor eine Erhöhung der CD73-Aktivität mit Abschaltung der Mitogene nachgewiesen werden. Diese Ergebnisse deuten auf eine Rolle des A2aAdoR bei der Bereitstellung mitogener Signale und einer damit verbundenen Gewährleistung des Überlebens dieser Zelllinie bei inaktivierten Mitogenen, sowie auf modulatorische Interaktionen zwischen der CD73 und A2aAdoR hin. Modulierte CD73-Aktivitäten und Beteiligung der AdoR an apoptotischen Vorgängen in der chronisch lymphatischen Leukämie sind bereits länger bekannt. Innerhalb einer klinischen Studie zur CLL wurden 48 Patienten und 10 Kontrollpersonen auf Modulationen der AdoR-, sowie c-myc- als auch ADA-Niveaus, untersucht und auf eine klinische Relevanz hin überprüft. Im Bezug auf den Mittelwert der Kontrollgruppe konnten oft Modulationen der mRNSNiveaus der untersuchten Komponenten innerhalb der Patientengruppe gefunden werden. Korrelationsanalyse der Patientendaten ergab verschiedene Zusammenhänge der AdoR, wie auch der c-myc und der ADA untereinander, die zum Teil gegenläufig zu denen in der Kontrollgruppe waren. A2aAdoR-Niveaus korrelierten negativ mit der Leukozytenanzahl, einem Marker der CLL. Es konnte auch ein deutlicher Zusammenhang zwischen der CD73-Aktivität und der Leukozytenanzahl in diesem Labor gezeigt werden. Ebenso fand man einen starken Zusammenhang zwischen den c-myc- und ADANiveaus, sowie zwischen der ADA bzw. c-myc und diversen AdoR-Subtypen. Diverse, aus physiologischen Zusammenhängen bekannte Korrelationen fand man auch in der CLL wieder, was auf eine geregelte Modulation der AdoR-Niveaus in der CLL hindeutet, wobei jedoch keine Zusammenhänge mit den prognostischen Stadien nach Binet gefunden werden konnten. Ein aktive Modulation des A2aAdoR bei der Expansion der malignen B-Lymphozyten konnte anhand der Ergebnisse von 4 Folgeuntersuchungen vermutet werden. Wiederholung der Untersuchung nach 6 Monaten bis zu 1,5 Jahren zeigte eine deutliche negative Korrelation der Leukozytenzahl mit dem A2aAdoR-Niveau.
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