Podcasts about Schwann

TWiN explains the role of a ubiquitin ligase component in regulating the sheathing of multiple axons by Schwann cells. Hosts: Vincent Racaniello, Vivianne Morrison, and Tim Cheung Subscribe (free): Apple Podcasts, Google Podcasts, RSS Links for this episode MicrobeTV Discord Server Gut microbiome promotes pain in fibromyalgia (Neuron) Timestamps by Jolene Ramsey. Thanks! Music is by Ronald Jenkees Send your neuroscience questions and comments to twin@microbe.tv

Siblings Ashley Engle and Brandon Birdwell discuss life, the Schwann's man, Ashely tries to explain Love is Blind (Brandon doesn't get it) and Crindy's Diamond Jubilee Celebration.

durée : 00:37:53 - La Terre au carré - par : Mathieu Vidard - En l'espace de quelques décennies, les œuvres fondatrices de Lamarck, Schwann, Darwin, Claude Bernard et Mendel vont dessiner le cadre général d'une nouvelle science, la biologie. Vous aimez ce podcast ? Pour écouter tous les autres épisodes sans limite, rendez-vous sur Radio France.

Monica and Mike positive spin frozen food! They also discuss favorite buttons, quartists, and the Schwann's man.

It's Saskatchewan Mining Week! It's an important week to celebrate and highlight this crucial industry in our province. Pam Schwann, president of the Saskatchewan Mining Association, joins Evan to chat about how the industry is doing, what's new and exciting and how Mining Week is being celebrated.

This week, the Maier's get together to discuss: bathroom reno, back to work, work/life balance, Schwann's, Conservative family, crossing the border, golf, SOW, Airhouse, and Spring playlist. Reach Us:  @kmaemaier  @chrismaierbc  @hwywhoney  hwywhoney@gmail.com

Matador (Afsnit 03) (1978) Kronologisk gennemgang af serien. www.janoghenrik.dk/ www.henrikogjan.dk/ Skiftedag. 1930 - Ikke alle klarer sig så godt som Mads Andersen-Skjern (Jørgen Buckhøj). Det gælder for eksempel indehaveren af Damernes Magasin, Albert Arnesen (Preben Mahrt), der bliver nødt til at invitere sin førstemand, Hr. Schwann, (Arthur Jensen), som netop har fået en mindre arv, ind som kompagnon, for at afværge en truende fallit. Arnold (Esper Hagen) bliver udlært og tager arbejde ovre hos Mads. 30'erne er netop startet - en tid med turbulens, ikke mindst på det politiske område. Dønningerne kommer til Korsbæk, hvor der agiteres højrøstet - både på den konservative og den socialistiske front. Mads gifter sig med Ingeborg (Ghita Nørby) og adopterer hendes datter Ellen (Helle Nielsen). Tekst fra Danskefilm.dk

A North Dakota couple, fans of the podcast, share some great stories from small town shenanigans in ND. The Schwann truck man creeps into a teepee and a Moose chases a kid down the dirt roads of Montana.

Today we're talking Schwann's dairy, Amazon Fresh, the rebirth of Hot & Now and beef jerky business cards?As always, find us here:https://www.speakpipe.com/InTheWeedsWithBenRandallhttps://www.facebook.com/groups/774902433251568https://www.instagram.com/chefbenrandall/ https://podcasts.apple.com/us/podcast/in-the-weeds-with-ben-randall/id869521547intheweedswbr.comhttps://www.redbubble.com/people/enzwell/shopintheweedswbr@gmail.com

Wisconsin's cattle industry's getting ready for some changes in animal identification standards.  ​Since 2013, breeding cattle over the age of 18 months, dairy cattle, and any cattle used for shows, etc. needed to have an official ID to move between states. This spring, USDA amended that rule so that the IDs have to be both visually AND electronically readable.  ​Stephanie Hoff finds out how it'll impact farmers with Tressa Lacy, president of the Wisconsin Cattlemen's Association​.Yellow trucks rolling in the driveway usually meant a treat for kids in rural Wisconsin.  Now it's going away.  Schwann's, now known as Yelloh, is ceasing operations by the end of November.Weather is really helping the state's farmers keep rolling in the harvest, but breakdowns can stop them in their tracks.  Getting big farm equipment fixed these days isn't easy.  Ben Jarboe talks to Danielle Waterworth, North America VP of Dealer and Customer Support for Case IH and New Holland.  She says they're turning to technology to help speed assistance. ​Consumers may not realize that the prices we share on the radio don't correlate to organic producers.  Shawna Nelson, executive vice president of membership with Organic Valley, says organic milk pricing stands on its own. Bryce Wyndecker, broker analyst with EverAg, joins Pam Jahnke to discuss the recent collapse in the barrel cheese price.  He also recaps recent milk production figures.See omnystudio.com/listener for privacy information.

Critical minerals are a key part of Saskatchewan's energy and resources plan. Last week, the province announced new incentives to grow Saskatchewan's mining sector further. Pam Schwann, president of the Saskatchewan Mining Association (SMA) joins Evan to discuss critical minerals in the province.

By special request - more info from the book The Invisible Rainbow by Arthur Firstenberg on the importance of the myelin sheath in transmission of energy throughout the body - and what can go wrong with Electromagnetic pollution.00:00 Intro to the book02:00 The importance of electrical energy in healing damaged tissue - porphyrins as transmitters of energy within cells - haemoglobin in red blood cells05:16 Electrical polarity of the brain can be reversed to cause anaesthesia, pain reduction and healing08:00 Porphyrins are energy transmitters in the mitochondria (as cytochrome c in the electron transport chain) but also those in the myelin sheath complexed with zinc, (chlorophyll uses magnesium, haem uses Iron). 11:00 With a porphyrin manufacture problem excess precursors builds up in tissue along with excess zinc causing hyper excitability of the neurons - predisposes to electro-sensitivity and chronic fatigue syndrome?13:06 Robert Becker's investigation in bioelectric healing in bone and nerves - electro-chemical transmission allows much of our activity16:25 'Currents of injury' - point to another information system that allows regeneration and healing18:17 Myelin sheaths/Schwann/glial cells are intimately involved in signal transmission and energy production in the brain and nervous system19:45 Electro-chemical sensitivity may interact with high zinc in brain and nerves, and other parts of body. EDTA (a chelators) complexes Zn and will take it out the body and can reverse cognitive decline22:00 90% of oxygen consumption occurs in brain from glial cells - myelin may be the major energy powerhouse completely independent of mitochondria24:02 Excess zinc is everywhere in the environment, car tyres a major factor along with denture cream, water supply, etc. 25:40 Are highly sensitive people (HSP's) the canaries in the coalmine with problems in the porphyrin pathways? What can we do to protect ourselves particularly during sleep and add highly coloured foods into our diet.*Watch another video**THE INVISIBLE RAINBOW PART 1 https://youtu.be/CYZFxL57paMTHE INVISIBLE RAINBOW PART 2 https://youtu.be/VgWSzIPBgHAHEAL YOUR SPIRIT TO HEAL CFS/ME https://youtu.be/hXGaOjrV6PAWHATS THE MATTER WITH MEDICINE - IAIN MCGILCHRIST https://youtu.be/CdE1QphYKoUHOW TO HEAL BIRTH TRAUMA WITH EMDR  https://youtu.be/JmtarRiGsgYEMOTIONAL RESILIENCE  https://youtu.be/E5TYi9dEBEUWHY ANXIETY IS NOT A MIND ISSUE https://youtu.be/Ty-J2pu37tAHOW CHILDHOOD EMOTIONS STILL AFFECT YOU: https://youtu.be/8LzR8yhR8u8 *If you're suffering from Chronic pain, fatigue or anxiety, I CAN HELP*CONTACT ME: https://www.alchemytherapies.co.uk/Alchemy Therapies & Emotional MasterclassOTHER USEFUL RESOURCESGroup Healing Program: http://myemotionalaudit.comAuthor/Book site: https//patriciaworby.comPodcast: https://www.alchemytherapies.co.uk/po...121 and group therapy and training for stress related conditions like anxiety, fatigue and pain: https://alchemytherapies.co.ukSee in particular: Thrive! - an introductory mindbody connection program and The Emotional Audit for more intensive training.COMING SOON:Intensive Training Program: https://emotionalmasterclass.com

Ein British Airways Airbus A320 am Flughafen Heathrow entging knapp einem großen Schaden, als eine mobile Treppe während der heißesten Tag des Jahres in Flammen aufging. Das Flugzeug war gerade aus Athen angekommen und wurde für den nächsten Flug vorbereitet, als das Feuer ausbrach. Glücklicherweise konnten die Reinigungskräfte das Flugzeug schnell verlassen und die Tür schließen, bevor die Flammen das Flugzeug erreichten. Die Feuerwehr konnte das Feuer schnell löschen, aber das Flugzeug wurde aus dem Betrieb genommen, um es zu überprüfen. Es gab keine Passagiere an Bord und keine Verletzten.#BritishAirways #A320 #Feuer #Heathrow #Flugzeug #Notfall #Sicherheit #Luftfahrt #NachrichtenFragen des Tages: Ist die Strafe von 12 Jahren gerecht? Wie findet ihr den "weissen Schwan" Boeing 787 von Austrian Airlines?00:00 Willkommen zu Frequent Traveller TV02:24 Boeing übernimmt bald Spirit Aerosystems05:14 Airbus muss Jahresziel reduzieren07:20 Geiselnehmer vom Hamburger Flughafen muss 12 Jahre in den Knast09:540 Austrian Airlines Boeing 787 “weisser Schwann” wird erst im Winter neu lackiert13:02 British Airways A320 entkommt Feuerschaden15:30 Skytrax 202419:32 World of Hyatt Promotion 1.000 Punkte und doppelte Statusnächte in FloridaFragen des TagesTake-OFF 25.06.2024 – Folge 144-2024 Kanalmitglied werden und exklusive Vorteile erhalten:https://www.youtube.com/channel/UCQyWcZxP3MpuQ54foJ_IsgQ/joinHier geht es zu eurem kostenlosen Consulting Link - https://FTCircle.as.me/Damit Du von unserem Wissen profitieren kannst, kannst du ein mindestens 60 minütiges und vor allem auf dich zugeschnittenes Punkte, Meilen, Status Coaching buchen. Nach dem Call bekommst du ein Jahr Zugang zu dieser Gruppe und zahlst so nur 10 Euro pro Monat und kannst sofort profitieren. Hier ist nun der Link zu deinem neuen Punkte, Meilen und Status Deals.MY SOCIALSWhatsApp - https://wa.me/message/54V7X7VO3WOVF1FACEBOOK | Lars F Corsten - https://www.facebook.com/LFCorsten/FACEBOOK | FQT.TV - https://www.facebook.com/FQTTVFACEBOOK | FTCircle - https://www.facebook.com/FTCircleTWITTER | Lars F Corsten - https://twitter.com/LFCorstenINSTAGRAM | Lars F Corsten - https://www.instagram.com/lfcorsten/LINKEDIN - https://www.linkedin.com/in/lfcorsten/Clubhouse - @LFCorsten

It's Saskatchewan Mining Week and this year's theme is critical careers for Saskatchewan's critical minerals. This week marks an opportunity to continue raising public awareness of the importance of the mining industry in the province and its benefits. Pam Schwann, president of the Saskatchewan Mining Association (SMA), and Guy Hiltz from the SMA's emergency response mine rescue competition committee join Evan.

This week on The Less Stressed Life Podcast, I am joined by the lovely August Brice of TechWellness. In this episode, August tells us how EMF (electromagnetic fields) affects our health and wellness. We talk about the research August has done and how to get the most bang for your buck when it comes to EMF protection devices. Get 15% OFF Digital Detox Collection with Code LESSSTRESSEDKEY TAKEAWAYS:My journey with the SomavedicWhat EMF Protection works - Chips & ShieldsHow to hardwire your internetNational Toxicology Study The findings of his research were most recently edified in 2018 by our government's own National Toxicology Program where links were found to cellphone radiation: Increased incidences of glioma, a rare, aggressive and highly malignant brain cancerIncreased incidence of schwannoma (a rare tumor of the nerve sheath) of the heartIncreases of these cancers were found in both sexes of rats, but reached statistical significance only in malesIncreased incidences of rare, proliferative changes in glial cells of the brain and in Schwann cells in the hears of bot sexes of rats while not a single unexposed control animal developed these precancerous changes.DNA damage was induced with both modulations of radiofrequency radiation (RFR) in both rats and mice (in the frontal lobe plus other tissues).ABOUT GUEST:August's passion is finding solutions for mindful living in our digital world. Her online platform, TechWellness.com is the only comprehensive online source that addresses all the challenges of our modern digital world - from EMF Radiation and effects of blue light, to Cyber security and mental wellbeing with tech. On top of her on-going research, August has built an Advisory Team consisting of world-renown experts in each area, who consult with Tech Wellness and review all major content on the site. All solutions are purchased and tested, never supplied by or sponsored by manufacturers to avoid any influence.WHERE TO FIND:Website: https://techwellness.com/ Instagram: https://www.instagram.com/techwellness/***WORK WITH CHRISTA***: https://www.christabiegler.com/fssWHERE TO FIND CHRISTA:Website: https://www.christabiegler.com/Instagram: @anti.inflammatory.nutritionistPodcast Instagram: @lessstressedlifeYouTube: https://www.youtube.com/@lessstressedlifeLeave a review, submit a questions for the podcast or take one of my quizzes here: https://www.christabiegler.com/linksEPISODE SPONSOR:A special thanks to Jigsaw Health for sponsoring this episode. Get a discount on any of their products! Use the code lessstressed10

Raghu Ramesh and John Svaren discuss their paper, “JUN Regulation of Injury-Induced Enhancers in Schwann Cells,” published in Vol. 42, Issue 34 of JNeurosci, with Megan Sansevere from SfN's Journals' staff. Find the rest of the Spotlight collection here. With special guests: Raghu Ramesh and John Svaren Hosted by: Megan Sansevere On Neuro Current, we delve into the stories and conversations surrounding research published in the journals of the Society for Neuroscience. Through its publications, JNeurosci, eNeuro, and the History of Neuroscience in Autobiography, SfN promotes discussion, debate, and reflection on the nature of scientific discovery, to advance the understanding of the brain and the nervous system.  Find out more about SfN and connect with us on Twitter, Instagram, and LinkedIn.  

„Ein Verb, das ist so, wie wenn man im dunklen Raum das Licht anknipst. Mit einem Schlag ist die Szene da.“ Hans Jürgen Heringer. 1984. Neues von der Verbalszene. In: Gerhard Stickel (Hg.). Pragmatik in der Grammatik. Düsseldorf: Schwann. 34-64, 49. Damit ist an und für sich und prinzipiell alles zur Idee der Valenzgrammatik gesagt -- aber wie bestimmt man die Valenz eines Verbs? Ich stelle verschiedene Möglichkeiten und digitale Ressourcen im Kontext der Beispielanalyse des Satzes "Versehentlich schickt sie den Brief an Storch.", der es in sich hat, vor. Die Vorlesung "Basiswissen Sprachwissenschaft" ist konzipiert als Überblicksvorlesung über die zentralen Sprachwissens- und Kommunikationsmodelle, die in der modernen Linguistik unser Bild von Sprachwissen und Kommunikation maßgeblich prägen. Präsentation (*.pdf): Alexander Lasch. 2023. Basiswissen Sprachwissenschaft. Zenodo. DOI: https://doi.org/10.5281/zenodo.8388691. Videoaufzeichnungen: https://youtube.com/@AlexanderLasch. Informationen & Material zu allen Vorlesungen: https://kurzelinks.de/fl7f. Intro: "Reflections" von Scott Holmes (CC BY via FMA). #Linguistik #OER #Sprache

In this episode of the Titans of Foodservice podcast, host Nick Portillo speaks with Scott Tomes, CRO of Bongards Creameries. Scott shares his remarkable journey in the food service industry, starting as a division manager at Schwann's. Today, he is a trailblazer in the industry, leading Bongard's to new heights of success. Scott discusses the challenges he faced, the strategies he implemented, and the valuable lessons he learned along the way. He also talks about the value of perseverance, leadership, and building strong relationships in the food service industry.Quotes“In today's competitive environment, you have to compete. But what is going to set you apart? That service level. The attention to detail. It's that relationship.” -Scott Tomes [09:22]“I don't want to put myself on a pedestal, but I tell my kids if you can pick up the phone, talk to someone as a human being and a person, you're going to already outflank 75% of your competition. I really believe that.” -Scott Tomes [29:02]TIMESTAMPS03:26 Scott's journey and role as a strike force division manager13:19 Varied sales training programs in food companies15:19 Lack of young talent in the food industry22:17 Techniques for hiring brokers and finding manufacturers28:04 Advice for aspiring chief revenue officers: Never stop learning34:11 COVID's impact on the food service industry38:41 Encouraging respect and open-mindedness in the industryRESOURCESPortillo SalesCONTACT Nick: nick.portillo@portillosales.comScott: https://www.linkedin.com/in/scotttomes/

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2023.07.25.550402v1?rss=1 Authors: Lowenstein, E. D., Misios, A., Buchert, S., Ruffault, P.-L. Abstract: The vagal ganglia, comprised of the superior (jugular) and inferior (nodose) ganglia of the vagus nerve, receive somatosensory information from the head and neck, or viscerosensory information from the inner organs, respectively. Developmentally, the cranial neural crest gives rise to all vagal glial cells and to neurons of the jugular ganglia, while the epibranchial placode gives rise to neurons of the nodose ganglia. Crest-derived nodose glial progenitors can additionally generate autonomic neurons in the peripheral nervous system, but how these progenitors generate neurons is unknown. Here, we found that some Sox10+ neural crest-derived cells in, and surrounding, the nodose ganglion transiently expressed Phox2b, a master regulator of autonomic nervous system development, during early embryonic life. Our genetic lineage tracing analysis revealed that despite their common developmental origin and extreme spatial proximity a substantial proportion of glial cells in the nodose, but not in the neighboring jugular ganglia, have a history of Phox2b expression. Lastly, we used single cell RNA-sequencing (scRNA-seq) to demonstrate that these progenitors give rise to all major glial subtypes in the nodose ganglia, including Schwann cells, satellite glia and glial precursors, and mapped their spatial distribution by in situ hybridization. Our work demonstrates that these crest-derived nodose glial progenitors transiently express Phox2b, give rise to the entire complement of nodose glial cells and display a transcriptional program that may underlie their bipotent nature. Copy rights belong to original authors. Visit the link for more info Podcast created by Paper Player, LLC

Uma rede de controle e integração de processos fisiológicos. Separe meia horinha do seu dia e descubra, com a Mila Massuda, o que é e como atua o nosso sistema nervoso.   Apresentação: Mila Massuda (@milamassuda)   Roteiro: Mila Massuda (@milamassuda) e Emilio Garcia (@emilioblablalogia)   Edição: @Matheus_Herédia (@mewmediaLAB)   Produção: Prof. Vítor Soares (@profvitorsoares) e BláBláLogia (@blablalogia)   REFERÊNCIAS:   JUNQUEIRA, Luiz C.; CARNEIRO, José. Histologia básica. 13ª edição. Rio de Janeiro - RJ: Guanabara Koogan, 2017.   KIM, Jong Kuk; LEE, Hye Jeong; PARK, Hwan Tae. Two faces of Schwann cell dedifferentiation in peripheral neurodegenerative diseases: pro-demyelinating and axon-preservative functions. Neural regeneration research, v. 9, n. 22, p. 1952, 2014.   LORACH, Henri et al. Walking naturally after spinal cord injury using a brain–spine interface. Nature, p. 1-8, 2023.   XING, Liujing et al. Wnt/β-catenin signaling regulates ependymal cell development and adult homeostasis. Proceedings of the National Academy of Sciences, v. 115, n. 26, p. E5954-E5962, 2018.

Olaparib + Abiraterone is FDA approved for mutated-BRCA prostate cancers. Olaparib has a bit of convoluted story to unpack in this patient population. Have we finally found a way to minimize the peripheral neuropathy from paclitaxel.....with cilostazol? PROpel: https://evidence.nejm.org/doi/full/10.1056/EVIDoa2200043 Cilostazol RCT: https://doi.org/10.1002/phar.2830 Cilostazol effect on Schwann cells: https://doi.org/10.1016/j.neuropharm.2021.108514

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2023.04.05.535701v1?rss=1 Authors: Nikolaev, Y. A., Ziolkowski, L. H., Pang, S., Li, W.-P., Feketa, V. V., Xu, C. S., Gracheva, E. O., Bagriantsev, S. N. Abstract: Mechanosensory corpuscles detect transient touch and vibratory signals in the skin of vertebrates, enabling navigation, foraging, and precise manipulation of objects1. The corpuscle core comprises a terminal neurite of a mechanoreceptor afferent, the only known touch-sensing element within corpuscles, surrounded by terminal Schwann cells called lamellar cells (LCs)2-4. However, the precise corpuscular ultrastructure, and the role of LCs in touch detection are unknown. Here we used enhanced focused ion beam scanning electron microscopy and electron tomography to reveal the three-dimensional architecture of avian Meissner (Grandry) corpuscle5. We show that corpuscles contain a stack of LCs innervated by two afferents, which form large-area contacts with LCs. LCs form tether-like connections with the afferent membrane and contain dense core vesicles which release their content onto the afferent. Furthermore, by performing simultaneous electrophysiological recordings from both cell types, we show that mechanosensitive LCs use calcium influx to trigger action potential firing in the afferent and thus serve as physiological touch sensors in the skin. Our findings suggest a bi-cellular mechanism of touch detection, which comprises the afferent and LCs, likely enables corpuscles to encode the nuances of tactile stimuli. Copy rights belong to original authors. Visit the link for more info Podcast created by Paper Player, LLC

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2023.04.02.535224v1?rss=1 Authors: Prior, R., Silva, A., Vangansewinkel, T., Idkowiak, J., Kumar Tharkeshwar, A., Hellings, T. P., Michailidou, I., Vreijling, J., Loos, M., Koopmans, B., Vlek, N., Straat, N., Agaser, C., Kuipers, T., Michiels, C., Rossaert, E., Verschoren, S., Vermeire, W., de Laat, V., Dehairs, J., Eggermont, K., van den Biggelaar, D., Bademosi, A. T., Meunier, F. A., vandeVen, M., Van Damme, P., Mei, H., Swinnen, J. V., Lambrichts, I., Baas, F., Fluiter, K., Wolfs, E., Van Den Bosch, L. Abstract: Duplication of PMP22 causes Charcot-Marie-Tooth disease type 1A (CMT1A) and is known to disrupt the lipid metabolism in myelinating Schwann cells by unknown mechanisms. By using two CMT1A mouse models overexpressing human PMP22, we discovered that PMP22 dose-dependently downregulates genes that are involved in lipid and cholesterol metabolism. Lipidomic analysis on CMT1A mouse sciatic nerves confirmed lipid metabolic abnormalities primarily associated with cholesterol and sphingolipids. We observed similar lipidomic profiles and downregulation of genes associated with lipid metabolism in human CMT1A patient induced pluripotent stem cell-derived Schwann cell precursors (iPSC-SCPs). We confirmed these findings by demonstrating altered lipid raft dynamics and plasma membrane fluidity in CMT1A iPSC-SCPs. Additionally, we identified impaired cholesterol incorporation in the plasma membrane due to altered lipid storage homeostasis in CMT1A iPSC-SCPs, which could be modulated by changing the lipid composition of the cell culture medium. These findings suggest that PMP22 plays a role in regulating the lipid composition of the plasma membrane and lipid storage homeostasis. Targeting lipid metabolism may hold promise as a potential treatment for CMT1A patients. Copy rights belong to original authors. Visit the link for more info Podcast created by Paper Player, LLC

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2023.03.18.533291v1?rss=1 Authors: Ptak, C. P., Peterson, T. A., Hopkins, J. B., Ahern, C. A., Shy, M. E., Piper, R. C. Abstract: Mutations in Myelin Protein Zero (MPZ) account for 5% of Charcot-Marie-Tooth cases and can cause demyelinating or axonal phenotypes, reflecting the diverse roles of MPZ in Schwann cells. MPZ holds the apposing membranes of the myelin sheath together, with the adhesion role fulfilled by the extracellular Immunoglobulin-like domain (IgMPZ), which can oligomerize. Current knowledge for how the IgMPZ might form oligomeric assemblies involving 3 weakly-interacting interfaces has been extrapolated from a protein crystal structure in which individual rat IgMPZ subunits are packed together under artificial conditions. These interfaces include one that organizes the IgMPZ into tetramers, a 'dimer' interface that could link tetramers together, and a third hydrophobic interface that could mediate binding to lipid bilayers or the same hydrophobic surface on another IgMPZ domain. There are at present no data confirming whether the proposed IgMPZ interfaces actually mediate oligomerization in solution, whether they are required for the adhesion activity of MPZ, whether they are important for myelination, or whether their loss results in disease. We performed NMR and SAXS analysis of wild-type IgMPZ as well as mutant forms with amino-acid substitutions designed to interrupt its presumptive oligomerization interfaces. Here, we confirm the interface that mediates IgMPZ tetramerization, but find that dimerization is mediated by a distinct interface that has yet to be identified. We next correlated CMT phenotypes to subregions within IgMPZ tetramers. Axonal late-onset disease phenotypes (CMT2I/J) map to surface residues of IgMPZ proximal to the transmembrane domain. Early-onset demyelinating disease phenotypes (CMT1B/Dejerine-Sottas syndrome) map to two groups: one is described by variants that disrupt the stability of the Ig-fold itself and are largely located within the core of the Ig domain; whereas another describes a surface on the distal outer surface of IgMPZ tetramers. Computational docking studies predict that this latter disease-relevant subregion may mediate dimerization of IgMPZ tetramers. Copy rights belong to original authors. Visit the link for more info Podcast created by Paper Player, LLC

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2023.03.18.533004v1?rss=1 Authors: Raut, N. G., Maile, L. A., Oswalt, L. M., Mitxelena, I., Adlakha, A., Sprague, K. L., Rupert, A. R., Bokros, L., Hofmann, M. C., Patritti-Cram, J., Rizvi, T. A., Queme, L. F., Choi, K., Ratner, N., Jankowski, M. P. Abstract: Pain of unknown etiology is frequent in individuals with the tumor predisposition syndrome Neurofibromatosis 1 (NF1), even when tumors are absent. Schwann cells (SC) were recently shown to play roles in nociceptive processing, and we find that chemogenetic activation of SCs is sufficient to induce afferent and behavioral mechanical hypersensitivity in mice. In mouse models, animals show afferent and behavioral hypersensitivity when SC, but not neurons, lack Nf1. Importantly, hypersensitivity corresponds with SC-specific upregulation of mRNA encoding glial cell line derived neurotrophic factor (GDNF), independent of the presence of tumors. Neuropathic pain-like behaviors in the NF1 mice were inhibited by either chemogenetic silencing of SC calcium or by systemic delivery of GDNF targeting antibodies. Together, these findings suggest that Nf1 loss in SCs causes mechanical pain by influencing adjacent neurons and, data may identify cell-specific treatment strategies to ameliorate pain in individuals with NF1. Copy rights belong to original authors. Visit the link for more info Podcast created by Paper Player, LLC

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2023.03.17.533188v1?rss=1 Authors: Handler, A., Zhang, Q., Pang, S., Nguyen, T. M., Iskols, M., Nolan-Tamariz, M., Cattel, S., Plumb, R., Sanchez, B., Ashjian, K., Shotland, A., Brown, B., Kabeer, M., Turecek, J., Rankin, G., Xiang, W., Pavarino, E. C., Africawala, N., Santiago, C., Lee, W.-C. A., Xu, C. S., Ginty, D. D. Abstract: Specialized mechanosensory end organs within mammalian skin -- hair follicle-associated lanceolate complexes, Meissner corpuscles, and Pacinian corpuscles -- enable our perception of light, dynamic touch. In each of these end organs, fast-conducting mechanically sensitive neurons, called A{beta} low-threshold mechanoreceptors (A{beta} LTMRs), associate with resident glial cells, known as terminal Schwann cells (TSCs) or lamellar cells, to form complex axon ending structures. Lanceolate-forming and corpuscle-innervating A{beta} LTMRs share a low threshold for mechanical activation, a rapidly adapting (RA) response to force indentation, and high sensitivity to dynamic stimuli. How mechanical stimuli lead to activation of the requisite mechanotransduction channel Piezo2 and A{beta} RA-LTMR excitation across the morphologically dissimilar mechanosensory end organ structures is not understood. Here, we report the precise subcellular distribution of Piezo2 and high-resolution, isotropic 3D reconstructions of all three end organs formed by A{beta} RA-LTMRs determined by large volume enhanced Focused Ion Beam Scanning Electron Microscopy (FIB-SEM) imaging. We found that within each end organ, Piezo2 is enriched along the sensory axon membrane and is minimally or not expressed in TSCs and lamellar cells. We also observed a large number of small cytoplasmic protrusions enriched along the A{beta} RA-LTMR axon terminals associated with hair follicles, Meissner corpuscles, and Pacinian corpuscles. These axon protrusions reside within close proximity to axonal Piezo2, occasionally contain the channel, and often form adherens junctions with nearby non-neuronal cells. Our findings support a unified model for A{beta} RA-LTMR activation in which axon protrusions anchor A{beta} RA-LTMR axon terminals to specialized end organ cells, enabling mechanical stimuli to stretch the axon in hundreds to thousands of sites across an individual end organ and leading to activation of proximal Piezo2 channels and excitation of the neuron. Copy rights belong to original authors. Visit the link for more info Podcast created by Paper Player, LLC

A new research paper was published on the cover of Aging (listed by MEDLINE/PubMed as "Aging (Albany NY)" and "Aging-US" by Web of Science) Volume 15, Issue 5, entitled, “AAV1.NT-3 gene therapy prevents age-related sarcopenia.” Sarcopenia is progressive loss of muscle mass and strength occurring during normal aging with significant consequences on the quality of life for elderly. Neurotrophin 3 (NT-3) is an important autocrine factor supporting Schwann cell survival and differentiation and stimulating axon regeneration and myelination. NT-3 is involved in the maintenance of neuromuscular junction (NMJ) integrity, restoration of impaired radial growth of muscle fibers through activation of the Akt/mTOR pathway. In this new study, researchers Burcak Ozes, Lingying Tong, Morgan Myers, Kyle Moss, Alicia Ridgley, and Zarife Sahenk from Nationwide Children's Hospital and The Ohio State University used a triple muscle-specific creatine kinase (tMCK) promoter to restrict NT-3 expression to the skeletal muscle and self-complimentary adeno-associated virus serotype 1 (scAAV1) as vector to assess the therapeutic efficacy of AAV1.NT-3 in wild type-aged C57BL/6J mice, a model for natural aging and sarcopenia. “Quantitative histopathologic parameters served to address age-related changes in muscle, peripheral nerve and NMJ.” The treatment efficacy was assessed at 6 months post-injection using run to exhaustion and rotarod tests, in vivo muscle contractility assay, and histopathological studies of the peripheral nervous system, including NMJ connectivity and muscle. AAV1.NT-3 gene therapy in WT-aged C57BL/6 mice resulted in functional and in vivo muscle physiology improvements, supported by quantitative histology from muscle, peripheral nerves and NMJ. Hindlimb and forelimb muscles in the untreated cohort showed the presence of a muscle- and sex-dependent remodeling and fiber size decrease with aging, which was normalized toward values obtained from 10 months old WT mice with treatment. The molecular studies assessing the NT-3 effect on the oxidative state of distal hindlimb muscles, accompanied by western blot analyses for mTORC1 activation were in accordance with the histological findings. “When considering the burden of sarcopenia on the lifestyle of elderly, and on the healthcare system, we believe this preclinical study is providing strong support for AAV.NT-3 gene therapy in the successful management of sarcopenia, as a serious and plausible option in the future.” DOI: https://doi.org/10.18632/aging.204577 Corresponding Author: Zarife Sahenk - zarife.sahenk@nationwidechildrens.org Keywords: sarcopenia, gene therapy, aging, NT-3, muscle remodeling Sign up for free Altmetric alerts about this article: https://aging.altmetric.com/details/email_updates?id=10.18632%2Faging.204577 About Aging-US Launched in 2009, Aging-US publishes papers of general interest and biological significance in all fields of aging research and age-related diseases, including cancer—and now, with a special focus on COVID-19 vulnerability as an age-dependent syndrome. Topics in Aging-US go beyond traditional gerontology, including, but not limited to, cellular and molecular biology, human age-related diseases, pathology in model organisms, signal transduction pathways (e.g., p53, sirtuins, and PI-3K/AKT/mTOR, among others), and approaches to modulating these signaling pathways. Please visit our website at https://www.Aging-US.com​​ and connect with us: SoundCloud - https://soundcloud.com/Aging-Us Facebook - https://www.facebook.com/AgingUS/ Twitter - https://twitter.com/AgingJrnl Instagram - https://www.instagram.com/agingjrnl/ YouTube - https://www.youtube.com/@AgingJournal LinkedIn - https://www.linkedin.com/company/aging/ Pinterest - https://www.pinterest.com/AgingUS/ Media Contact 18009220957 MEDIA@IMPACTJOURNALS.COM

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2023.03.01.530693v1?rss=1 Authors: Shi, Y., Sun, S., Li, J., Wang, S., Yu, Y., Guo, K., Yang, J., Han, L., Wei, W., Qiu, J. Abstract: Introduction: Understanding the cellular composition and trajectory of human tooth development is valuable for dentistry and stem cell engineering research. Previous single-cell studies have focused on mature human tooth and developing mice tooth, but cell landscape on human embryonic dental development is still lacking. Objective: We aimed to construct the spatiotemporal cell atlas of aborted fetus tooth germ. Methods: We collected tooth germ tissues from aborted fetus (17-24 week) for single cell RNA sequence and spatial transcriptome. Subsequent clustering, spatial projection, pseudotime, gene regulation network, pathway enrichment and signaling network analysis were applied to reveal the cellular composition as well as its biological significance. Results: We classified all cells into seven subclusters of epithelium, seven clusters of mesenchyme and other cell types like Schwann cell precursor and pericyte. For epithelium, the matrix cell-striatum intermedium branch and the ameloblast branch diverged from a same set of KRT15+-HOPX+-ALCAM+ epithelial stem cell lineage, but the spatial distribution of two branches were not clearly distinct. This trajectory received spatially adjacent regulation signals from mesenchyme and pericyte, including JAG1 and APP. The differentiation of pulp cell and pre-odontoblast showed four waves of temporally distinct gene expression, which involved regulation networks of LHX9, DLX5 and SP7 and were regulated by upstream ligands like BMP family. Conclusion: We provided a reference landscape for the research on human early tooth development, covering different spatial structures and developmental periods. Copy rights belong to original authors. Visit the link for more info Podcast created by Paper Player, LLC

Jason read an article by Tom Silverstein in the Milwaukee Journal Sentinel, and he thought "Spoon" and Tausch were on the same page about something Brian Gutekunst said in Indianapolis. Wilde & Tausch Trivia, including reminiscing on yesterday's tough performance. And Homer joins for his weekly appearance one day after Marquette clinched its first Big East regular season championship ever...and he claims he's not talking about Aaron Rodgers until there's actual news?

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2023.02.27.530298v1?rss=1 Authors: Grove, M., Kim, H., Pang, S., Amaya, J. P., Hu, G., Zhou, J., Lemay, M., Son, Y.-J. Abstract: The hippo pathway transcriptional effectors, YAP/TAZ, are crucial for Schwann cells (SCs) to myelinate axons but the mechanisms are poorly defined. Although TEAD1 has been implicated as a partner transcription factor, how it supports the transcriptional regulation of myelination and which aspects of the process it affects are unknown. Here, using conditional and inducible knockout mice, we report that TEAD1 is essential for SCs to develop, grow, and regenerate myelin sheaths. TEAD1/2/3/4 are present in SCs, but YAP/TAZ strongly favors TEAD1. It promotes myelination by positively and negatively regulating SC proliferation, enabling Krox20/Egr2 to upregulate myelin proteins, and upregulating the cholesterol biosynthetic enzymes FDPS and IDI1. We also show stage-dependent redundancy of TEAD1 and that non-myelinating SCs have a unique requirement for TEAD1 to enwrap nociceptive axons in Remak bundles. Our findings establish TEAD1 as a crucial partner of YAP/TAZ in developmental myelination and functional nerve regeneration and as a novel transcription factor regulating Remak bundle integrity. Copy rights belong to original authors. Visit the link for more info Podcast created by Paper Player, LLC

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2023.01.24.525436v1?rss=1 Authors: Vasilijic, S., Atai, N., Hyakusoku, H., Worthington, S., Ren, Y., Sagers, J. E., Sahin, M. I., Fujita, T., Landegger, L. D., Lewis, R., Welling, B. D., Stankovic, K. M. Abstract: Vestibular schwannoma (VS) is intracranial tumor arising from neoplastic Schwann cells, causing hearing loss in about 95% of patients. The traditional belief that hearing deficit is caused by physical expansion of the VS, compressing the auditory nerve, does not explain the common clinical finding that patients with small tumors can have profound hearing loss, suggesting that tumor-secreted factors could influence hearing ability in VS patients. Here, we conducted profiling of patients plasma for 67 immune-related factors on a large cohort of VS patients (N greater than 120) and identified candidate biomarkers associated with tumor growth (IL-16 and S100B) and hearing (MDC). We identified the 7-biomarker panel composed of MCP-3, BLC, S100B, FGF-2, MMP-14, eotaxin, and TWEAK that showed outstanding discriminatory ability for VS. These findings revealed possible therapeutic targets for VS-induced hearing loss and provided a unique diagnostic tool that may predict hearing change and tumor growth in VS patients and may help inform the ideal timing of tumor resection to preserve hearing. Copy rights belong to original authors. Visit the link for more info Podcast created by Paper Player, LLC

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2023.01.05.522786v1?rss=1 Authors: Walker, L. J., Guevara, C., Kawakami, K., Granato, M. Abstract: A critical step for functional recovery from peripheral nerve injury is for regenerating axons to connect with their pre-injury targets. Reestablishing pre-injury target specificity is particularly challenging for limb-innervating axons as they encounter a plexus, a network where peripheral nerves converge, axons from different nerves intermingle, and then re-sort into target-specific bundles. Here, we examine this process at a plexus located at the base of the zebrafish pectoral fin, equivalent to tetrapod forelimbs. Using live cell imaging and sparse axon labeling, we find that regenerating motor axons from three nerves coalesce into the plexus. There, they intermingle and sort into distinct branches, and then navigate to their original muscle domains with high fidelity that restores functionality. We demonstrate that this regeneration process includes selective retraction of mistargeted axons, suggesting active correction mechanisms. Moreover, we find that Schwann cells are enriched and associate with axons at the plexus, and that Schwann cell ablation during regeneration causes profound axonal mistargeting. Our data provide the first real time account of regenerating vertebrate motor axons navigating a nerve plexus and reveal a previously unappreciated role for Schwann cells to promote axon sorting at a plexus during regeneration. Copy rights belong to original authors. Visit the link for more info Podcast created by Paper Player, LLC

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2022.12.14.520389v1?rss=1 Authors: Catasus, N., Torres-Martin, M., Negro, A., Kuebler, B., Rosas, I., Casals, G., Mazuelas, H., Roca-Ribas, F., Amilibia, E., Aran, B., Veiga, A., Raya, A., Gel Moreno, B., Blanco, I., Serra, E., Carrio, M., CASTELLANOS, E. Abstract: Background: The appearance of bilateral vestibular schwannomas (VS) is one of the most characteristic features of NF2-related schwannomatosis (NF2-related SWN), an autosomal dominant syndrome that predisposes to the development of tumours of the nervous system. VS are caused by the bi-allelic inactivation of the NF2 gene in a cell of the Schwann cell lineage. Our current understanding of VS initiation and progression as well as the development of new effective therapies is hampered by the absence of human non-perishable cell-based models. Principal Findings: We generated and characterized induced pluripotent stem cell (iPSC) lines with single or bi-allelic inactivation of NF2 by combining the direct reprogramming of VS cells with the use of CRISPR/Cas9 editing. Despite the difficulty of maintaining merlin-deficient iPSCs, we were able to differentiate them into neural crest (NC) cells. At this stage, these cells showed spontaneous expression of the SC marker S100B and the impossibility of generating Schwann cells in 2D cultures. Nevertheless, by applying a 3D Schwann cell differentiation protocol, we successfully generated NF2(+/-) and NF2(-/-) spheroids homogeneously expressing classical markers of the NC-SC axis. Conclusions: Our results show a critical function of NF2 for both reprograming and maintaining a stable pluripotent state. In addition, merlin-deficient cultures also denoted an altered differentiation capacity of merlin-deficient cells towards the NC-SC axis, in the in vitro conditions used. Finally, the generated NF2(+/-) and NF2(-/-) spheroids show potential as a genuine in vitro model of NF2-related tumours. Copy rights belong to original authors. Visit the link for more info Podcast created by Paper Player, LLC

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2022.12.12.517822v1?rss=1 Authors: Carnicer-Lombarte, A., Barone, D. G., Fawcett, J., Franze, K. Abstract: Spinal cord injuries have devastating consequences for humans, as mammalian neurons of the central nervous system (CNS) cannot regenerate. In the peripheral nervous system (PNS), however, neurons may regenerate to restore lost function following injury. While mammalian CNS tissue softens after injury, how PNS tissue mechanics changes in response to mechanical trauma is currently poorly understood. Here we characterised mechanical rat nerve tissue properties before and after in vivo crush and transection injuries using atomic force microscopy-based indentation measurements. Unlike CNS tissue, PNS tissue significantly stiffened after both types of tissue damage, likely mainly due to an increase in collagen I levels. Schwann cells, which crucially support PNS regeneration, became more motile and proliferative on stiffer substrates in vitro, suggesting that changes in tissue stiffness may play a key role in facilitating or impeding nervous system regeneration. Copy rights belong to original authors. Visit the link for more info Podcast created by Paper Player, LLC

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2022.12.08.519209v1?rss=1 Authors: Fazal, S. V., Mutschler, C., Chen, C., Turmaine, M., Chen, C.-Y., Hsueh, Y.-P., Loreto, A., Casillas-Bajo, A., Cabedo, H., Franklin, R. J., Barker, R. A., Monk, K., Steventon, B., Coleman, M., Gomez-Sanchez, J. A., Arthur-Farraj, P. Abstract: SARM1 is a central regulator of programmed axon death and is required to initiate axon self-destruction after traumatic and toxic insults to the nervous system. Abnormal activation of this axon degeneration pathway is increasingly recognized as a contributor to human neurological disease and SARM1 knockdown or inhibition has become an attractive therapeutic strategy to preserve axon loss in a variety of disorders of the peripheral and central nervous system. Despite this, it remains unknown whether Sarm1/SARM1 is present in myelinating glia and whether it plays a role in myelination in the PNS or CNS. It is important to answer these questions to understand whether future therapies inhibiting SARM1 function may have unintended deleterious impacts on myelination. Here we show that Sarm1 mRNA is present in oligodendrocytes in zebrafish but only detectable at low levels in Schwann cells in both zebrafish and mice. We find SARM1 protein is readily detectable in murine oligodendrocytes in vitro and in vivo and activation of endogenous SARM1 in oligodendrocytes induces cell death. In contrast, SARM1 protein is not detectable in Schwann cells and satellite glia in the adult murine nervous system. Cultured Schwann cells contain negligible functional SARM1 and are insensitive to specific SARM1 activators. Using zebrafish and mouse Sarm1 mutants, we show that SARM1 is not required for initiation of myelination nor myelin sheath maintenance by oligodendrocytes and Schwann cells. Thus, strategies to inhibit SARM1 function in the nervous system to treat neurological disease are unlikely to perturb myelination in humans. Copy rights belong to original authors. Visit the link for more info Podcast created by Paper Player, LLC

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2022.12.07.519441v1?rss=1 Authors: Fuentes-Flores, A., Geronimo-Olvera, C., Necunir-Ibarra, D. S., Patel, S. K., Bons, J., Wright, M. C., Geschwind, D., Höke, A., Gomez-Sanchez, J. A., Schilling, B., Campisi, J., Court, F. A. Abstract: After peripheral nerve injuries, successful axonal growth and functional recovery requires the reprogramming of Schwann cells into a reparative phenotype, a process dependent on the activation of the transcription factor c-Jun. Nevertheless, axonal regeneration is greatly impaired in aged organisms or after chronic denervation leading to important clinical problems. This regenerative failure has been associated to a diminished c-Jun expression by Schwann cells, but whether the inability of these cells to maintain a repair state is associated to the transition into a phenotype inhibitory for axonal growth, has not been evaluated so far. We find that repair Schwann cells transitions into a senescent phenotype, characterized by diminished c-Jun expression and secretion of factor inhibitory for axonal regeneration in both aging and chronic denervation. In both conditions, elimination of senescent Schwann cells by systemic senolytic drug treatment or genetic targeting improves nerve regeneration and functional recovery in aging and chronic denervation, associated with an upregulation of c-Jun expression and a decrease in nerve inflammation. This work provides the first characterization of senescent Schwann cells and their impact over axonal regeneration in aging and chronic denervation, opening new avenues for enhancing regeneration, and functional recovery after peripheral nerve injuries. Copy rights belong to original authors. Visit the link for more info Podcast created by Paper Player, LLC

My guest today is a true savage, and the definition of a renaissance man. Not only does Tommy Schwann run the regional division of Cardinal Financial, but he owns and manages 30 rental properties, he owns a ticket broker busines, he invests in a sports medicine company, and he's been involved in countless other businesses over the years. This is one of the most riveting episodes we've had, so you don't wanna miss it. --- Send in a voice message: https://anchor.fm/charles-weinraub/message

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2022.10.31.514415v1?rss=1 Authors: Kantarci, H., Elvira, P. D., Thottumkara, A. P., Iyer, M., Donovan, L. J., Ambiel, N., OConnell, E. M., Granados, A., Zeng, H., Saw, N. L., Lutz, A. B., Sloan, S. A., Gray, E. E., Tran, K. V., Vichare, A., Yeh, A. K., Munch, A. E., Huber, M., Agrawal, A., Morri, M., Shamloo, M., Tawfik, V. L., Du Bois, J., Zuchero, J. B. Abstract: Excitability, the ability to fire action potentials, is a signature feature of neurons. How neurons become excitable during development, and whether excitability is an intrinsic property of neurons or requires signaling from glial cells, remain unclear. Here we demonstrate that Schwann cells, the most abundant glia in the peripheral nervous system, promote somatosensory neuron excitability during development. We find that Schwann cells secrete prostaglandin E2, which is necessary and sufficient to induce somatosensory neurons to express normal levels of voltage-gated sodium channels and fire action potential trains. Inactivating this signaling pathway specifically in Schwann cells selectively impairs nociceptor and proprioceptor somatosensory neuron subtypes, leading to corresponding sensory defects in thermoception, inflammatory pain, and proprioception. Our studies thus reveal a cell non-autonomous mechanism by which glia regulate neuronal excitability to enable the development of normal sensory functions. Copy rights belong to original authors. Visit the link for more info Podcast created by Paper Player, LLC

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2022.10.28.514178v1?rss=1 Authors: Jiravejchakul, N., Abe, G. L., Loza, M., Park, S., Matangkasombut, P., Sasaki, J.-I., Imazato, S., Diez, D., Standley, D. M. Abstract: In-depth knowledge of the cellular and molecular composition of dental pulp (DP) and the crosstalk between DP cells that drive tissue homeostasis or regeneration are not well understood. To address these questions, we performed data analysis of publicly available single-cell transcriptomes of DP. This analysis revealed that DP resident fibroblasts have a unique gene expression profile when compared with fibroblasts from 5 other reference tissues: blood, bone marrow, adipose tissue, lung, and skin. Genes coded for heparin-binding growth-factors, pleiotrophin (PTN) and midkine (MDK), possessed the highest differential expression levels in DP fibroblasts. In addition, we identified extensive crosstalk between DP fibroblasts and several other DP cells, including Schwann cells, MSCs and odontoblasts. These findings suggest that fibroblast-derived growth factors regulate DP niches, and thus have a potential role as dental therapeutic targets. Copy rights belong to original authors. Visit the link for more info Podcast created by Paper Player, LLC

Coolio died. National Covfefe Day. Pizza Pthursday. See omnystudio.com/listener for privacy information.

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2022.09.25.509350v1?rss=1 Authors: Daboussi, L., Costaguta, G., Gullo, M., Pessino, V., O'Leary, B., Lettieri, K., Driscoll, S., Pfaff, S. L. Abstract: Schwann cells respond to acute axon damage by transiently transdifferentiating into specialized repair cells that restore sensorimotor function. However, the molecular systems controlling repair cell formation and function are not well defined and consequently it is unclear whether this form of cellular plasticity has a role in peripheral neuropathies. Here we identify Mitf as a transcriptional sensor of axon damage under the control of Nrg-ErbB-PI3K-PI5K-mTorc2 signaling. Mitf regulates a core transcriptional program for generating functional repair Schwann cells following injury and during peripheral neuropathies caused by CMT4J and CMT4D. In the absence of Mitf, core genes for epithelial-to-mesenchymal transition, metabolism and dedifferentiation are misexpressed and nerve repair is disrupted. Taken together, our findings demonstrate that Schwann cells monitor axonal health using a phosphoinositide signaling system that controls Mitf, which is critical for activating cellular plasticity and counteracting neural disease. Copy rights belong to original authors. Visit the link for more info Podcast created by PaperPlayer

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2022.08.25.505298v1?rss=1 Authors: Willows, J. W., Gunsch, G., Paradi, E., Blaszkiewicz, M., Tonniges, J. R., Pino, M. F., Smith, S. R., Sparks, L. M., Townsend, K. L. Abstract: Peripheral neuropathy is a pathophysiological state of nerve degeneration and loss of tissue innervation. The most prominent cause of small fiber neuropathy is diabetes which can be demyelinating in nature, but this has not yet been explored in adipose tissue. Both demyelinating neuropathies and axonopathies implicate Schwann cells (SCs), the peripheral glial required for nerve myelination and regeneration after injury. Here, we perform a comprehensive assessment of SCs and myelination patterns of subcutaneous white adipose tissue (scWAT) nerves, including changes that occur with obesity and other imbalanced energy states in mice and humans. We found that mouse scWAT is densely innervated by both myelinated and unmyelinated sensory and sympathetic nerves. Accordingly, scWAT is home to both myelinating and non-myelinating SCs; the greater proportion of which are myelinating. Furthermore, SCs were found closely associated with synaptic vesicle-containing nerve terminals in scWAT. Obese BTBR ob/ob mice exhibit diabetic peripheral neuropathy in scWAT, and display concordant demyelination specific to small fibers, which was also associated with a decrease in the pan-SC marker Sox10 and compensatory increase in Krox20 gene expression. Together this suggests that adipose SCs may be involved in regulating the plasticity or the neuropathy of adipose tissue nerves. Copy rights belong to original authors. Visit the link for more info Podcast created by PaperPlayer

A new research paper was published in Oncotarget on July 19, 2022, entitled, “CUDC907, a dual phosphoinositide-3 kinase/histone deacetylase inhibitor, promotes apoptosis of NF2 Schwannoma cells.” Neurofibromatosis Type 2 (NF2) is a rare tumor disorder caused by pathogenic variants of the merlin tumor suppressor encoded by NF2. Patients develop vestibular schwannomas (VS), peripheral schwannomas, meningiomas, and ependymomas. There are no approved drug therapies for NF2. Previous work identified phosphoinositide-3 kinase (PI3K) as a druggable target. Researchers Julianne Huegel, Christine T. Dinh, Maria Martinelli, Olena Bracho, Rosa Rosario, Haley Hardin, Michael Estivill, Anthony Griswold, Sakir Gultekin, Xue-Zhong Liu, and Cristina Fernandez-Valle, from the University of Central Florida and the University of Miami Miller School of Medicine, conducted an unbiased chemical compound screen of the Library of Pharmacologically Active Compounds (LOPAC) as a pilot high-throughput screen to identify NF2 schwannoma targets for inhibition. “Here we screened PI3K pathway inhibitors for efficacy in reducing viability of human schwannoma cells.” The lead compound, CUDC907, a dual histone deacetylase (HDAC)/PI3K inhibitor, was further evaluated for its effects on isolated and nerve-grafted schwannoma model cells, and primary VS cells. CUDC907 (3 nM IG50) reduced human merlin deficient Schwann cell (MD-SC) viability and was 5–100 fold selective for MD over WT-SCs. CUDC907 (10 nM) promoted cell cycle arrest and caspase-3/7 activation within 24 hours in human MD-SCs. Western blots confirmed a dose-dependent increase in acetylated lysine and decreases in pAKT and YAP. In a 14-day treatment regimen, CUDC907 decreased tumor growth rate by 44%, modulated phospho-target levels, and decreased YAP levels. In five primary VS, CUDC907 decreased viability, induced caspase-3/7 cleavage, and reduced YAP levels. Its efficacy correlated with basal phospho-HDAC2 levels. CUDC907 has cytotoxic activity in NF2 schwannoma models and primary VS cells and is a candidate for clinical trials. “In summary, we demonstrated that CUDC907 reduced the activity of three major signaling pathways in NF2 schwannomas (HDAC, PI3K, and YAP) and consistently reduced viability and induced apoptosis in several schwannoma cell models and in all five genetically unique primary VS studied. These consistent results offer the possibility that CUDC907 will promote schwannoma regression in patients with diverse NF2 mutations and support clinical evaluation of CUDC907 for NF2-associated schwannomas and potentially other cancers driven by NF2 pathogenic variants [45]. Current use of this drug in clinical trials for other indications reveals clinical interest in multi-modal drugs over monotherapies.” DOI: https://doi.org/10.18632/oncotarget.28254 Correspondence to: Cristina Fernandez-Valle – Email: cfv@ucf.edu Keywords: fimepinostat, Schwann cell, vestibular schwanomma, merlin, nerve allograft About Oncotarget: Oncotarget (a primarily oncology-focused, peer-reviewed, open access journal) aims to maximize research impact through insightful peer-review; eliminate borders between specialties by linking different fields of oncology, cancer research and biomedical sciences; and foster application of basic and clinical science. To learn more about Oncotarget, visit Oncotarget.com and connect with us on social media: Twitter – https://twitter.com/Oncotarget Facebook – https://www.facebook.com/Oncotarget YouTube – www.youtube.com/c/OncotargetYouTube Instagram – https://www.instagram.com/oncotargetjrnl/ LinkedIn – https://www.linkedin.com/company/oncotarget/ Pinterest – https://www.pinterest.com/oncotarget/ LabTube – https://www.labtube.tv/channel/MTY5OA SoundCloud – https://soundcloud.com/oncotarget For media inquiries, please contact: media@impactjournals.com.

In this episode of the Lexman Artificial Podcast, we discuss the consistory, Schwann cells, asyndetons, and heterostyly. Simon Sinek, founder and CEO of The4Hs, joins us to discuss how these simple structures protect our cells and how they can be improved to better serve the organism.

(09-15-2017) - Ralph Holguin joined Randy from his garage in Long Beach, Ca. and talked about cars he grew up in, his fascination with classic Schwann "Sting Ray" bikes like the "Apple Crate" and his classic builds and TV shows....and the unusual name his family gave one of his parents' cars.

And then there were 4! 4 teams remain after the NFL weekend and 4 Hosts break it down as Chris Little, aka “Miner 49er” joins the Boys. Justin takes us down memory lane of the Schwann's food delivery truck, and Dylan pays homage to HOF Coach Sean Peyton retiring. --- Send in a voice message: https://anchor.fm/robert-brandt5/message

  Vidcast:  https://youtu.be/ZJlHdKNwpVU   Covid19 can now be added to the family of virus capable of infecting and damaging inner ear cells that provide our hearing and sense of balance.  Investigators at MIT and Harvard's Mass. Eye and Ear Infirmary now report this finding that may explain why some CoVid patients report hearing loss, ringing in their ears or tinnitus, and balance problems.   The investigators studied cellular models of the inner ear and, in limited cases, actual human inner ear tissues obtained from patients undergoing surgery for other reasons including cancer.  The cellular inner ear models were initially developed to study viruses known to cause deafness including cytomegalovirus mumps virus, and hepatitis virus. To create these models, stem cells were derived from skin cells and stimulated to differentiate into typical inner ear cells including hair cells, nerve cells, and supporting cells.   Studies show that inner ear cells including the all-important hair cells and nerve-insulating Schwann cells possess the ACE2 receptor proteins that permit CoVid infection.  A clinical study of 10 CoVid patients with ear-related symptoms found that all had mild to profound hearing losses, 9 had tinnitus, and 6 had dizziness. The prospect of hearing loss and dizziness are yet 2 more reasons to avoid getting CoVid and you know how you do that….. vaccination, masking, and ventilation.   https://www.nature.com/articles/s43856-021-00044-w   #CoVid #deafness #vertigo #tinnitus #ear  

Optimal Health Strategist Gunther Mueller has developed what he calls the 'Magnetic Mind Method." This means he helps people tap into our subconscious to create and manifest the life we've always wanted.=============================================Ari Gronich0:14Welcome back to another episode of creating a new tomorrow. I am your host Ari Gronich today with me is Gunther Mueller. And Gunther is a certified magnetic mind coach now, you know, I'm gonna let him explain that but 30 years of successfully being an entrepreneur, optimal health strategist, you have, you know, you have three kids alongside all the work that you've done, but you've actually taken and builds a business in three years to $20 million in the anti-aging, I believe, field, founded another company, you've bought and sold several companies. So today, we're going to talk a lot about not just the health and wellness, not just the mindset, but also kind of the deep and down and dirty parts of business a little bit. And I'm going to kind of take you on a journey today audience that hopefully will lead you into a place where you could go, Oh, yeah, I got this. And I can move ahead tomorrow, creating my new tomorrow, today. So anyway, Gunther, tell us a little bit about yourself.Gunther Mueller1:24Hey, Ari, thank you so much for having me on, create a new tomorrow. It's amazing to be here because the title of your show is completely in alignment with the information I'm passionate about sharing today. And hopefully the audience gets a lot out of this today. So a little bit about me and I grew up middle class in New York City, bolted out of there in 1984 to go skiing in Colorado and go to school out there because scheme was my thing and need to go ski the bigger mountains but you know, did the thing that you were supposed to do get good grades, go to college, you know, do that whole rigamarole thing. And then I became a professional ski bum for four years after college. So I lived in Vail, lived in a steamboat for a while and commercial fish in Alaska, worked on the Valdez oil spill. If anybody remembers what that was, I was in Prince William Sound for about 60 days, moving people around and equipment and things like that. And then I started my sales career basically in the 90s, selling meat and seafood door to door because I had experience in the seafood industry. You know, I knew what good stuff was. I built about 3000 customers in the Colorado mountainous region. This is the days before Sam's Club and before you know, Costco and all that.Ari Gronich2:41So I just want to say this. So when I was 17 and a half 18. And I'm just finishing up school, high school and rural Oregon. Right? Yeah, I was selling meat and seafood door to door in Oregon on the back of a truck with a freezer on the back of a truck just like a regular big old freezer, laying in the bed of a truck. And so. Gunther Mueller3:14Hey I did it for 10 years. And I loved it because I got to wear shorts and a golf shirt every day. And I had great customers all over the place and loved it. And then I turned it into an online company in 1998 and then sold that company to one of my suppliers. And then I got into the restaurant business for 14 years. I had about four restaurants that I managed and so food was kinda in my blood food distribution. I work for a we'll start up coffee roaster and then I created America's freshest coffee for the Schwann food company for a while. I went to go to the corporate gig as a regional vice president for them managed a million square mile territory did really well. But the corporate world was not of my liking or choosing. So I you know, get this entrepreneurial blood in my in my veins. And I think I got that from my mom, she know how to sell. She's a travel agent for 50 years, and just knew how to get people to go great places, right. And so then after that I've been in the solar industry did really well used to sell $4 million a month worth of solar panels. And then from solar. I got into the medical industry, which I've been in for over 10 years now. And that's where I created that company and about three years doing about 20 million a year and it was really changing the paradigm of medicine with your average ob-gyn and family practice doctor to optimize hormones and optimize nutrition instead of being so pharmaceutically based. I mean it was really a quantum shift in medicine for a lot of people I was really specialized in something called pellet therapy, which was getting hormones actually inserted into the body and it's you know, it's everywhere now, but when I did it 10 years ago, nobody knew what a pellet was. It was, so I was kind of one of the spear hitters of that therapy in the United States.Ari Gronich5:06Very cool. So nowadays, you know, you're not doing that exactly. You're, you're doing this thing called the magnetic mind. Right, coach. Now, I want to get into this a little bit. So how did you get started working with mind? How did mindset play a role in your sales? So I'm kind of doing a multi question here. So how did mindset play a role in your sales? How did you get into mindset? I know for a friend of mine, oh, he was with Xerox for a while, and they had Zig Ziglar, and all these sales training. So just kind of that background. And then what made that turn into what you're doing now? And how do you see this as kind of that next evolution? Gunther Mueller5:54Yeah, great question Ari, I love answering it. So what happened was in those days of selling meat and seafood, like I was always a true seeker, even from being a little kid, you know, I used to go walk by a church and think, oh, God lives there. You know what I mean? And but how does that all work out? What's the reality of the universe? Basically, I want to know how things work, right? And nobody really was able to answer it for me. And so in my days of selling meat and seafood door to door, my vehicle was my university, I listened to not the radio or pop music or anything, I listened to the greats like Zig Ziglar. You know, one of my favorite quotes from Zig Ziglar is you can have anything in life if you help enough other people get what they want. You know, and he was a great guy. And, you know, the Brian Tracy's of the world, the Tony Robbins of the world. Look, I what I'm here to share today, I did not create, right, I stand on the shoulders of giants, okay, who have investigated every aspect of personal development, human consciousness, you know, the whole quantum physical research over the last 40 years, there's so much science behind understanding the power that we have in our mind. But it all started with reading, thinking Grow Rich, it was one of the first books and it's the quintessential text, you know, in, let's call it getting what you want in life, or, you know, creating a new tomorrow, like, how do you do it, you know, you're living your life, and you want something different, you want something better. And we're gonna talk about that a little bit later. But you want something different, you want something better, there's a difference between the two ideas on so I started doing that one book after another one cassette tape after another really dating myself there, right? cassette tapes was the thing. And then the DVDs, and I used to drive 100, 200 miles a day. So all that education, all that content, all of that listening to a different way to think about things. And that kind of got embedded in my cellular structure from all those years of doing that. And today, I think the magnetic mind method is really a revolution in the personal development space. Because I'm at the place today to tell everyone that look, you're not broken. There's nothing for you to fix. And a lot of the history of the personal development movement has always been going back, to fix yourself to do something to get something right, something's broken inside of your personality, or something's broken in your being, and you have to fix it first in order to get what you want. I'm here today to tell everyone that we look we need to back out of the problem-solving reality and move into the creator stance. And the creator stances that power position. It's like, we need to remember who we truly are that we are connected to an infinite field of possibilities. And when we become consciously creative, we can create whatever situation reality manifest anything that we want. And this is backed up by science.Ari Gronich8:54So I'm gonna ask you, I'm gonna, I'm gonna go back a step. I'm gonna ask you a question you may not have heard before. So I started doing asked when I was eight, life spring, landmark forum, I mean, Cyworld, MIT. I've done so many of these self-improvement movement, workshops and programs and weekends and events and things. What I watch, what I observe, is about 90 so odd percent of the people go there are motivated for about two weeks to a month, and then it dies down. 5% start following the practices that they hear and maybe last a year or two or three until some trauma, gets them out of it. And then there's about 4%, right, that really buy in and get the information and then about 1% or so. This is being this is just my statistics and my, my, what I've watched that actually like live, the information that they've been taught. So here's my question to you. You have done all of these things. And you've taken it. And you've actually become I don't know which percentage but one of the 10, let's say, part of that 10% of the people, right? What makes you have that ability versus say, somebody else? What do you think is the difference between what you were able to do with the information and technology and experiences that you received? That you think the 90% of people who don't ever shift haven't gotten? What do you think that break is that delineation?Gunther Mueller10:55The break is truly listening to your own voice inside your head, okay, because especially today in the era of social media, we are so enamored or concerned with what other people think. I mean, it's getting to the point of ridiculousness, where our self-esteem if we don't look out for it is really coming from what other people do, do they like us, do they share us, do they do this kind of thing, right? And so back that when you asked me that question, the first thing that pops up in my mind is, I have had the ability to listen to my own voice. Now, I'm not saying that everything that I've done has been successful. Look, the path to success is laced with failure. And it's in failure, that you learn the most important lessons, if you had nothing but success in life, you would not be very seasoned, you would not be very skilled, you would not be very proficient in anything. It's through failure, it's through challenge. And this is really the human experience. A lot of people will say, look, I'd like to have a life with just no problems. I'm telling you, you'd be bored out of your mind, if you had no problems, okay? If there were no challenges, no problems, nothing to deal with in life, you would be bored out of your skull. That's just not why we're here as human beings, we're going to have this human experience. Now the beautiful place to be is to be consciously creative to kind of be an observer of what's going on, you know, an airplane at 30. 40,000 feet can see the landscape, right. And when you have that observer mentality, but this takes some practice, this takes some training, right? They don't teach it in school, they don't teach it in college, most of your parents don't teach it to their kids. Unless you become a hungry seeker to a degree and find this out for yourself and your percentages, I agree with so many people get information, they get knowledge. But look, the power is in the knowledge applied. You can go course after course, book after book, seminar, after seminar, do all these retreats, do all kinds of thing, like you said, you feel good for about a month. And then you just forget because you have not applied. And so then the second piece, listening to your own inner voice, because look, you know what if your desires are, let's call it God's plan for your life. What if those desires, What if those things that voice that's trying to speak to you is the directional signal in your life, and you keep ignoring it, you don't listen to it, you never take any time, you've got noise blaring at you all the time, and you never listen to the little voice that's inside and then trusted enough to follow it and not worry so much about what others may think of you. Correct? That's one of the key points right there.Ari Gronich13:51I'm pondering that because there's definitely a level of truth to I think that people go home after getting motivated. And then, you know, somebody says, Well, that wasn't probably what you know, like, or that's not going to work or that's not you know, that you get excited about what you're doing. So I can understand that. I think it goes a little deeper into the depths of the psyche, though. So that concept that you've stated of worrying about what other people think of you, right? goes deeper. So let's drop down into a deeper level of that.Gunther Mueller14:33Love it. So to go deeper is that we all have some self-sabotaging identities that we have acquired through this, let's call it the life stream of this life. And it really is impactful from like zero to seven years old, you know, the data and the science tells us that that's when we just really have an open mind. And we are trying to figure out how it is here. We're trying to figure out You know how to get love. We're trying to figure out how to get nourishment. We're trying to figure out how to get a safe place to sleep. We're trying to figure out how to get what we want, when we're in that stage of development. And so we make certain decisions about life about how it is here. That's all it is. It's just we're trying to figure out what's it like here? And how do I survive. And so if you have abuse, or if you have trauma, or you have some episodes in your life that are unpleasing, the human reality is that we avoid pain. And we move to pleasure. But we avoid pain, a hell of a lot more than we move to pleasure. So what the reality is, is mediocrity becomes okay. Because it's not painful, right? It's just tight, I'm not in excruciating pain. I'm not in a, you know, ecstasy or pleasure. So I am okay with mediocrity. And the part of our mind, we have the conscious mind, the subconscious mind, and what I'm going to introduce to you is the superconscious mind, and there's different names for that, but we like to call it the superconscious. In the subconscious programming, we have put things in there to prevent pain or to keep us safe. And the job of the egoic mind that conscious mind is to maintain the status quo. The conscious mind does not like change, because it knows how to navigate what is successfully. Right. And so some of the sabotaging identities that we pick up through a lifetime of experience, is things like I'm not good enough. I'm not worthy. I'm not capable, meaning I don't know enough, I'm not. This is an example of someone that never gets out of school. And they continually go for the next degree and the next degree in the next degree, right? I'm not, I'm just not capable of any one more thing. And then I'll be okay. I'm insignificant, I'm small, I'm not big enough, right, I'm insignificant, I'm not perfect. Many of us have this perfectionist stream in our mind can't do that, till I perfect this, this has to be just absolutely perfect before I get what I want. And then another big one is I don't belong; this is what we just talked about is this belonging. And it's okay to belong, it's okay to have a great tribe and a cool group of people, but you still have to be you. And so in light of the probably the top six self-sabotaging identities, and everybody has one or two of them, or all six of them in different degrees that we've incorporated into our subconscious program. And I want, I want you to be thinking about the subconscious like Windows 10 on your computer, okay, when you turn on your computer, Windows 10, boots up, the thing just runs, you don't know how it's running, you don't know the code, you don't know anything like every once in a while an update gets sent to Windows NT update, and you restart. And now the program is different than it was before. So we have to do the same thing to our subconscious program, because it's running completely unconsciously. And we put things in there to keep us safe. So when we when I say we need to step out of the problem solving reality, and take the creator stance, most of the audience is saying probably what does he mean by that? Right? What do you mean by a creative stance? Let me give you four examples of what I'm going to call true choices. And…Ari Gronich18:16First let's go through what problem solving is. Right? And then we'll go into that because we've gone through an automatic response system, which is your conscience, right.Gunther Mueller18:31So problem solving is what we've always been trained to do. We want what we want. So how do we get what we want? So the problem is to figure out a way to get what we want, and we do it consciously.Ari Gronich18:45So you're saying that the problem is wanting something that we don't have?Gunther Mueller18:51Yeah, but that's not the problem. Your desire, your desire is totally fine. You can desire and want whatever you want. That's not the wrong part. The part is that we've been trained to figure out in our conscious mind, how to solve the problem of not having it. Right. One having it is not the problem. It's the way we go about getting it anything comes the problem. So to think about goal setting, right? We've been taught to set SMART goals, and you got to have a date on it. You got to be clear about what you want. And then there's 5, 6, 10 steps or whatever to get what you want. And those things have to happen by a certain date. So when you do a SMART goal, you have in your own conscious mind figured out how it needs to happen. You have allowed no space for the field of infinite possibilities to provide the solution to you in some let's call it magical way. Okay, so you've spent your conscious energy your mind solving the problem. Let's take the idea of abundance, financial abundance. Right. Let's create a new tomorrow. And my two choices, I want to have the experience of having more than enough. I just want this experience of financial abundance and abundance in all aspects of my life. That's my true choice. I just always want to be in the experience of having more than enough. Well, how do I do that? Being in the entrepreneurial world, I deal with a lot of entrepreneurs that have decided or chosen that they need a big successful business in order to have that. And I always have to put the brakes on a little bit and say, Look, the business may not be the true choice. What your true choice is, is you want to have the experience of abundance. Having a successful business made give you the experience of massive struggle, okay, if you don't start a business, having the end in mind, you will get to a completed business that potentially you might hate it, you might not want, it may dominate your life. I mean, how many business owners are there where the business owns them? They don't own the business. Right? So be careful what you ask for Be careful what you wish for. Because if you do it in that problem-solving thing, you're looking at it from a field of limited possibilities. And when I say step out of the problem-solving thing, it's focusing on what you want, not on how it's going to show up, not on how it's going to manifest not on the how to how truly is up to the infinite field and the superconscious. Because look, abundance could happen by finding $100 million in a suitcase on the street. You could find it floating in the ocean, you want. And when we talk about infinite possibilities, I mean, infinite possibilities, whatever your imagination could imagine, and how abundance could show up for you. It's possible. But our conscious mind rationally goes in and say, Well, if you have these sabotaging identities, they're well, I'm not worthy of that that's never gonna happen to me, or I'm not good enough. That can never happen to me or I don't have enough knowledge. I don't have this. I'm insignificant, too small. I would never find that suitcase. Like, I'm just not lucky. You know what I mean? Like I do, I've walked right by the suitcase, and I'd miss it. And I would never find it right. So the programming and the tape that's running in that subconscious mind really rules the day. And so did I answer your question about what I mean by problem solving?Ari Gronich22:30Yes. And I just want to kind of get into what I heard was Basic Law of Attraction, right? So going to the experience that you want to experience, you know, whether it's visioning and feeling all the feelings of the perfect day or all, you know, those kinds of things. So that's cool. Because obviously, I want to experience the abundance of life fully, never needing or wanting anything, just everything is available at all times. Right. Now, the key thing that I believe was missing from the law of attraction was the step of action. Now, within what you just said, the confines of what you said is, we're not doing the SMART goal where we're creating the necessary actions from a problem solving point of view, we're going into the infinite. How does one get to the action side from that place?Gunther Mueller23:31So that's the fifth step in the five-step magnetic mind method. So it's the last thing we do? And we asked the question, okay, what is the next obvious action? And that you're right, that's where the secret, you know, great shows and opening the mind to a lot of possibilities and the power of the mind. And why I always like to say is the law of attraction, the secret is trying to solve the problem from the conscious mind. So this is where affirmation is. And I'm not saying they're wrong. And I'm not saying they don't work. They just take time. And they take that discipline, as you said in the beginning, right? People feel great for a month and they do it, and then it Peters off. Why is that? Because they don't see instantaneous results. Which is another concept I just want to throw in here as a seasoning real quick is the idea of as soon as possible. You see when you use a SMART goal, and you put a date on it, and the date goes by and it didn't happen. What most people do. Give up, or quit. Oh, well, didn't happen. I guess goal setting must not be for me. Goal setting doesn't work for me. Right? I tried, it doesn't work. So take any of the great personalities that we look to an Elan musk or you know, Prince or Madonna or you know, any of these celebrity type people that we look at. You think they have ever had to pick themselves up and try again, and try again, and keep going. Keep going for what they loved. Kept going like you look at Richard Branson, right? Just the other day he got into space. I mean, how long is that dream been manifesting, for him, of putting together all the engineers and you know, the concept laced with failure. And he's does other things and he's failed just as much as he succeeded in his life, maybe even failed a little bit more than he's succeeded, right. Way more. And he is not a perfect personality, right? If you got to know any of these people, they are not perfect beings in every aspect of their life, there is not, but they went after what they love to do, they went after that desire and focused on nothing else, you know, taken Oprah Winfrey or something like that, you know, built her media empire, she focused on what she loved, and she had perfect human, the perfect individual of No. And that's where this whole idea of perfection and all that comes in these things, we just have to let go. Right. That you have to let these things go. And there's a process to doing that. But when we try to solve the problem from the conscious mind, we're bumping into that subconscious programming. And what I'm going to share with you is how we go from the superconscious side, we just send an update to the subconscious, we do that with something called recode. Where we go in, we send an update, and we don't need to know what the problem was, we don't need to know what created the problem. We don't need to know if it was mommy or daddy or a teacher or some other situation going on. Right?Ari Gronich26:27So this sounds very different than, say, a bug fix for a software update, where when you go through the update, now all of a sudden, all the programs start acting wonky, you know, and then you get the blue screen of death. So we don't want to have the blue screen of death with our with our upgrades, right? We want to have the bugs, you know, eliminated. So how do we do the difference between those two, right? How do we get the upgrade to be smooth?Gunther Mueller26:59We do that because you're super conscious self, this highest version of yourself that is connected to the field, the infinite field and a great book to read on the field as Lynne McTaggart book just called the field. So much research has been done. We as human individuations are all part of this field, whether you're conscious of it or not. Okay, you're connected. And we are all connected. And if you look into the science, you look at all the experiments that have happened, we've proven this the field exists. So we're just going to take that as a given for the moment. If you don't believe me, you don't trust me, do your own research, dig in, right? got the field. And so we're connected into the field. So when you go to the superconscious level superconscious already knows what's happened in the past superconscious already knows all the connections knows all the dynamics. And when we do read code, we're basically asking for what we want. We say superconscious do you see the desire? Do you see the true choice? Do you see these two choices of experiencing infinite abundance? And when you connect into the field superconscious will respond usually in Yes, no answers. That's why you always ask questions in the yes and no type field, right? And, yeah, I see it. And then we go through a process of creating a structural tension, where the tension because the mind likes to resolve tension. And it likes to do it in a way that it's the path of least resistance. And so resistance is really the thing that keeps us from having what we want. And it is the identity structure that is congruent with the current reality. So Principle number one really is we have to take responsibility for the way it is now. And that's probably a big stepping stone that many people may have to get over. And that you I want to say this, you know, I say I'm gonna teach you how to become superconscious The truth is you already are. And you've already created everything that you're experiencing right now. So you are already a superconscious great, and now you just created some stuff that you might not like.Ari Gronich29:06I want to go back a little bit so you had said something regarding I just had it in my head a second ago. It was I love that I can edit these videos. It's so nice. Alright, keep going and I'll get back to it.Gunther Mueller29:32So we were on this track. Now I lost the track while we were talking aboutAri Gronich29:38Superconscious. Talking about superconscious going from above. Oh, I know what it was. So resistance. So I have a little bit different take on the resistance. Sustained resistance is what stops you. spurts of resistance are what drive you forward. And I'll tell you what I mean by that is the resistance in a lobster shell is what makes them want to go get another show. Right? It's that uncomfortable place that launches them into that next place. And so that's where I just want to, I want to delineate, at least for me, if thing is sustained resistance, if you let the resistance go, if you never change the shell, and you just keep building the resistance, yes, that is going to stop change. For me, the resistance is the signal that says change is needed now. And let's do that.Gunther Mueller30:32So I would equate that piece that you're saying that that is the true choice. That is the desire when you get to that place. And you've been, let's say, living this Groundhog Day reality, because there's only three places we can be, we can be stuck. We can be what we call oscillating, oscillating feels like three steps forward, two steps back one step forward, one step back, right, we're oscillating all we can be in a flow state flows, where we turn thoughts into things, and anybody that's done any high level athletics or anything like that are seeing the interviews with top athletes, they get into the zone. And they can make that three-point shot because they've done it a million times before and they're just in that zone, it just Swish, right? That's the zone feeling. And we can do that in our lives where we just turn thoughts desires into things. And I want to touch on this real quick. Well, how does that happen? As manifestation happened? The idea is, is that you're actually collapsing a part of the field into the present moment experience. So of the field of infinite possibilities, we're focusing on one possibility, with consistency. And the field actually collapses into the present moment. This is manifestation, this is how it happens. And it's photons is the smallest particles in the quantum physical reality. And the experiments that have proven This is that the particles don't even exist until the scientist intends to observe them. Meaning that the particle shows up for the experiment, when the observer intends to measure it, accelerate it, do whatever they're going to do with it to test it out. That's when the particle actually shows up. So the same thing happens in our manifestation. And when we have a true choice, we have a true desire. And we're focusing on that not trying to solve the problem, but we're focused on what we want. And we recode the resistance out of the way from the superconscious level, that true choice shows up as soon as possible. I'm not saying it's going to show up tomorrow.Ari Gronich32:40Got it. So that's where the as soon as possible comes in from the SMART goals. So we've kind of wrapped around. So let's get into that that as well. When we say something like, as soon as possible, kind of like one of the things that I say is how can it get any better than this? It's an open-ended question, right? That has no specifics to it, that allows the conscious mind to solve its own problem. Right. So here's the here's the question to you is, isn't that problem solving? Or is that something else? Gunther Mueller33:18Well, I was just going to stop and say it's not the conscious mind doing the problem solving when we're doing what we're doing is we're just asking superconscious to recognize the resistance, it's back to the resistant your piece of resistance, I would equate to being the true choice and the desire, that sustained resistance is the sabotaging identity. Okay, that's what creates the oscillating. And it just feels like you know, many times I've had what I wanted, I've been there. It's like, when I've created companies, I get there to the end, I have it life's good. Got the cash flow, get everything. There's still something missing. I wasn't really clear enough about what I want here. So my self-sabotaging reality was I could create anything I could build stuff. My thing was, I wasn't good enough to keep it. Yeah, I was great. I was creator, I could do this. I could build anything. But then when it was completely built and humming and running, you got taken away from me, or something happened and it cratered. But that's the underlying identity. Because the identity has to be congruent with the reality. If your identity never changes from like, I'm not good enough to I am good enough. I am capable, I am worthy. If that never changes, you can create a bunch of things and they won't sustain this happens in relationships. This happens, you know, in intimate love relationships, like you get there. It's the best thing in the world and the whole thing, just craters and goes away and you got to start over. What is that? Right That's what we're talking about here. So that resistance is in the center. unconscious program, it is a self-sabotaging identity. And so we can create it through affirmation and conscious work and all that. But it takes a long time to do that. And it takes diligent effort on our part to do it consistently. And so why I think the magAri Gronich35:18We're a fast food nation. So you know, that's been, you know, when I look at cognitive behavioral therapy, and the old paradigm of trauma work, I look at this long process, lifelong process of question and discovery, as to why your mind feels a certain way about a certain thing. I mean, I was seven when I was sent to my first psychologist, right. And I look at that as such a primitive way of doing therapy. Whereas, like, back in the, in the day, you know, tribal societies used plant medicines, and used tribal and cultural togetherness, deal with people's stuff. So let's accelerate what you're talking about. So we're going to accelerate from this old paradigm of subconscious moving things. So we're going to go to the superconscious and accelerate things. What does that look like?Gunther Mueller36:25Yeah, so I'm going to share that. But I don't want to say like everything that's been is not bad. Okay. We do the best we know how to do with what we know. And seven-year-old ongoing see the psychotherapist and he says the best that maybe your parents or whoever you had to do at the time, those were the tools, right? So think of everything is huge evolution that's happening. And this is awakening to the place that we're at today. And today, we have something called the magnetic mind method where, you know, what if it could be easy, what if it doesn't take 10 years of psychotherapy to figure out why I am the way that I am, and why I can't have what I want, or I get what I want, and it gets taken away. So when we go to superconscious, superconscious already knows. And we don't need to spend all that time digging in and asking the questions and figuring out where the connections were and where the misalignments were in Well, you know, I thought something but it wasn't really true. And I had, I gotta straighten all this out. superconscious can straighten that out in a blink, just because it already knows and you don't have we don't have to tell it any of the details, all we have to do is focus on what we want. And it's really the experience of what we want. So you mentioned earlier about, you know, getting into the emotions, getting into the emotion of the end result is step three, and the five step method because, you know, Einstein said, Look, there's only two things in the universe, there's information and there's energy, the information is the desire, the what, what do I want, okay. And the energy is the emotion. And it's like a holographic movie that when those two things come together, it's actually how a hologram is created. Okay, the energy and the information come together and shoot the manifests a hologram. So think of your life as like a holographic movie, where you are manifesting, you are, things are showing up in real time. And think of yourself for a second, as you're the director, you're the producer, you're the screenwriter, you've handed everybody their parts, and everything is happening, not to you, but for you to have the experience that's congruent with your identity. So you get treated by the characters, you know, as Shakespeare said, you know, all the world's a stage, and we're just actors on it, right? But you're the main guy, even in a movie, imagine walking into the screen and you becoming the main character. And when you look at some movies or series or something like that, some characters get written out a script. Right, they die off something terrible happens, they no longer exist. And the whole dynamic of the movie changes Think of your life in that way. The people that are there the circumstances, the conditions, the what is now is just what is. And when we focus on something else. And is the key point here also, we can focus on the problem, we can focus on how to fix the problem, and try to create, invent or figure out how to solve the problem. But what we focus on grows. So the more we focus on the actual problem, the bigger the problem sometimes gets. That's where we have to back out of that go into the creator stance and focus on what we would love focus on what we would just purely want. And that's how you know you have a true choice. If I asked you why do you want what you want? And you give me an answer and it sounds like a stepping stone on to something else. As a coach, I'm going to tell you that's not really the true choice because you're choosing something to get something else we have to get to the final end result. So I want to share just four creative stances with you real quick, to give you the perspective, a good creator stance is something like I choose to live my true nature and purpose. I just choose it. I choose to live my true nature and purpose because I'm going to tell you the only power that we really have in life is the power of choice. Think about it from the moment you wake up in the morning, what time do I get up? What are we going to wear, when we're going to go, we're going to drive, I'm going to take a bus, you know, when am I going to take lunch, it's a series of choices. And every choice has a result, or call it a consequence, right? So I choose to live my true nature and purpose. Another one is I choose to be the predominant creative force in my life.Gunther Mueller0:00I choose to live the life that I love. And this comes in alignment with your actions, right? The person that is living a life that they love, or this imaginative person that you see right now living a life that they love and the desire with that emotion of the end result, you're seeing the life that you love. What would you be doing right now, that's in alignment with that true choice. The action has to become an alignment, the identity needs to shift, but the actions have to be in alignment with their true choice. In other words, I choose to be healthy and vital. You know, the health issues we have going on in this country in the world and all that, you know, when your body is not working, and supporting you in the life that you love, it's a problem, you don't get to do the things that you love to do, because your body's not cooperating. So having a true choice, and I choose to be healthy and vital. And so let's just take a serious condition right now, if you're dealing with cancer of some level, the two choice is not to be cancer. The true choice is to be healthy and vital to have the experience. It's not the problem solving of how do I beat cancer? What therapy Do I need to beat cancer and all that the mindset shift needs to be creative and say, I choose infinite health and vitality? And what would it feel like to be infinitely healthy and vital. And you get into that stance? Because I'm going to tell you that everything that's ever been created, has been created twice, once in the mind. And once in a three-dimensional physical experience.Ari Gronich1:31Yeah, you know, it's funny, because I watched a lot of Jim Rohn stuff. And one of the things that Jim Rohn says is, is you wouldn't build a hotel until it was done. Right. You wouldn't build the thing until you had the blueprints until it was done. In your mind. If you just started to build something, you had brick, and you didn't know what you were building, people would ask you, you know, what are you building? I don't know, I'm just putting bricks together and they'd send you away. You know, he's like we are human beings are the one species that can program in and pre plan and choose what they're going to create. And…Gunther Mueller2:19sees are on instinct right. They're instinctual beings. Right? We have this creativeness. And if you ever read scripture in the beginning, I mean, it starts out right in the beginning says we were created in the image of the Creator. And so if we were in the image of the Creator, what are we? We are creative?Ari Gronich2:40I mean, if you're religious and believe that that is the line, absolutely. If you're not religious, and you don't believe that that's the line in a book, that means anything, it's still we create our kids, right? We create our imagination; I tell people on this show a lot. Like, we made this shit up. This is all a figment of our imagination. All of it, every single thing that we see here, taste do, everything is a figment of our imagination.Gunther Mueller3:17And the science backs that up. Our thoughts are perceptions and illusory, they're illusion, our emotions are illusion, they're not real. Okay, we make you sad. It's a simply we make this shit up, we create the reality we experience. And that's why you already are a superconscious creator. And all we have to do is what are you focused on? Are you focused on solving the problem to get what you want? Or do you really take back your power as a creator and choose to be the predominant creative force in my life? Ari Gronich3:56So we're gonna go back to your sales background a little bit, okay? Is what you just said? Ring a picture in my head of a billboard with a sign that says buy something to do something to get somewhere, right? So people are watching social media, advertising, how do they even know what is their true choice? How would they how would you even at this level, in this day and age, right, the bombardment of information and problems and stuff, right? How does somebody get to what that true choice is and while avoiding the noise of the sales of that advertisingGunther Mueller4:52Great question, because that is step one. In the five step magnetic mines. How do you choose a true choice? How do you actually get to it? And a true choice. The simple answer is if I asked you like, give me something you would just love. Give me something you would just really want. What's something?Ari Gronich5:09I'll just go to the, you know, question that life spring always or landmark always asked is chocolate or vanilla? Okay, for ice cream, like, what do you choose, chocolate or vanilla. Gunther Mueller5:21Choice of chocolate or vanilla or the choice of chocolate? Doesn't really doesn't matter. One day, I'll choose chocolate one day, I'll choose vanilla, because I like variety. Right? Okay, so that but that choice doesn't have any consequences. Right? So let's say let's say somebody chooses, let's take it in business, right? Um, you know, be like, Ari if you're coaching them are those on the show today, I got to start this business because I'm sick and tired of my nine to five job and I'm tired of my boss, I want to work for myself, you know, and they've seen the glitz on social media of people who've made it big, and they're driving lambos and stuff like that, you know, and you're just like, I want that, I want that. But the only way I'm going to get that I'm not going to get that at my job doing what I'm doing right now. Because my boss is cheap, and he's never gonna pay me more. I'm not getting paid what I want, what I'm worth, you hear the story that goes on mouth is a story, right? And so they would come to a coach like myself or like you, right? And we'd be like, well, I'm gonna do this, I need help doing this. And I'm gonna ask the question, Well, why do you want that? And if the answer is not just because I want it, it's not a true choice. If the answer becomes I want it, because when I have it, then I can be this or I can get that or it can become something else. Or it gets me to another place, then that thing that you just told me you wanted is not the true choice. It's just a stepping stone on to what you really want. So a true choice gets answered with I want it just because I would love to experience that. I want it just because I want it my being my desire, I just want that. I don't care what anybody else thinks. I don't get anybody else's input, whether it's a good choice, bad doesn't matter. I want it because I want to experience it experience is a very important thing. Because it's maybe not be a thing. It may not be something, it may be just an experience, like infinite abundance, or, you know, optimal vital health. Right?Ari Gronich7:30So true choice. I still, and I just want you to go deeper, I guess into it. I still see. Let's say I want joy, I want infinite joy. I want to experience joy at will. Gunther MuellerWhy do you want that? Why do you want to experience joy? On an infinite level? Ari GronichRight? That's what I'm saying is like, if somebody's saying that there's, at least in my case, it would be cuz I don't, but it would be. I haven't experienced enough joy in my life. So I want to experience at will the experience of joy. I love watching joy when I watch American Idol and I see somebody win. And they're just like, sheer joy. I want that. Right? It never, it never seems like a true choice. Because there's always is an outside perspective or an outside. If it's something I have not experienced, right, then it's outside of me. It's something I've been told would be good, right?Gunther Mueller8:35Your key right there, it's something I've been told would be good. And I should go do that. I should want that. That would be good for me. Someone else said. And then somebody else says that somebody else says somebody else says because all these somebody else's said it, it must be true. And it's not. So that's why coming into two choices and exercise that I do. It's called seven levels deep. And so you say the first thing that you really want, whatever it is, and I'm gonna ask you. So when you get that, what does that give you? Well, what do you get when you get that? What does that do for you? You say? Well, when I get that, I'm going to get this and it goes down to the next level. Okay, so when you get that, what does that do for you? But what do you get when you get that? Well, when I have that, then it's going to give me this. Okay, take that down. You have to third level now, right? You do that for seven levels deep. I want this because it gets me that then well, why do you want this? Well, because when I have this I can have that. And when I have this then I can have that and he push it down about seven levels and when you get down to the very bottom, and a lot of times you need a coach to do this because people will immediately say I don't know. And a coach will be like you do know you are connected to your infinite field that infinite consciousness. You do know, there's an aspect there's a resistance of you that doesn't want to recognize that, you know, because there may be a latent fear there, there may be something there that's blocking that, that real connection. And so it's a great exercise to go seven levels deep and Okay, so I say I want this thing, what do I want that? Okay, when I get that was like, What do I want that and you take it all the way down, that's how you get what I really want, is the experience of freedom. And no one ever getting to tell me what the hell to do. That's what I really want. Freedom, like for me is one of the operative words that have pushed me through life is the word freedom. And I was when I was in Alaska, I was working on a boat called the Born Free. no coincidence. Okay, the Born Free. And that's I identified with that name right away, like I am born free. It's not a I choose to statement like I knew it my consciousness that I am born free, free to choose what I want, when I want, who I want to do it with how much of it I want to do, it's me. And some people will flip that around while you just being selfish. No, it's in that same vein, that I can help whoever I want, I can provide for whoever I want, I can do all things with that type of freedom. And so when you look at the human desires of what it is we truly want, and you do a seven levels, deep exercise like that, I can tell you're going to get to the nitty gritty of what it is you really want. And that leads me to the two most important questions in life, which is Who are you? Who is it that you say you are? How do you operate? you operate with honesty, integrity, you know, things like that, like how do you I want to give you all the words, but how do you describe who is it that you say you are Who are you? And most of us have not spent any time contemplating that question, Who am I really Who am I? And then the second question is, what do I want, based on who I am what do I want. And all the social media, all the noise, all the influence from parent's school programming, peer pressure, whatever you want to call it, all that noise needs to cease for a moment, or lots of moments. So that you can actually get into your own being and understand what it is you truly would choose just because you would love it. See, we've never been given the opportunity in our programming really, to choose from a place of love. We choose from a place of elimination, sometimes, well, I got three crappy choices. Okay, so get rid of that one, get rid of that, I guess I choose that we choose by default, because we don't see any other choices, I don't have any. So I got to do that. And we choose by consensus. Before I make a decision, let me check with everybody and make sure everybody's gonna be on board with my decision. That's not a true choice. Where the fear is, if I choose something, my friends don't agree with me, I'm gonna lose my friends. That's fear. Right? So be conscious, observe how you choose what you choose. And that's a practice also, that's something that we just have to become conscious of? And what is our motivation? What are we really? Why do we want what we want? Is it to impress others? Is it to be liked? is it to have this feeling of belonging? is it to have this feeling of significance or being capable or admired, or to be beautiful or to be whatever, right? Whatever that desire is, it's a process of becoming conscious now, we don't have to go back and unravel everything because we are not broken. What is, is, and this is another key point I want to share the future will not be better. Many of us to say my life will be better when, my life will be better when this happens, or that happens when I get this, then I'll be able to do that. And everything is contingent on the future showing up. That's not how you create because the future is not going to be better because you are still going to be you in the future. Okay, what's it's just going to be different. And if we can just hold that thought for a second, the future is not going to be better. It's just going to be a different experience. And what I'm experiencing right now is just what is it's not bad. It's not horrible, because we just naturally our conscious mind like to throw labels on stuff. This suck. That's bad. That's wrong da da da.., right? And I want this because it's gonna be better. It's not going to be better. It's just going to be different experience than what is now If we can hold that for a second, we can achieve a level of contentment in the present moment, we can just be okay with what is. And we can just observe the current reality. And what is right now as just that is just what is and I choose something different. The feel the feel the difference of that it's not a half two, it's not anything like that it's I want, I just, I'm okay with where I am right now. It's just what it is. I created it all anyway. And I'm just choosing a different experience.Ari Gronich15:37Right. So that kind of ties into the Create a new tomorrow, you know, ideal is, as we started off with at the beginning of this is how to create a new tomorrow today. How do we? How do we get out of our own way? How do we, you know, stop the madness, you and I started before we hit record, we started talking about kind of what's going on in the world. I mean, the president of Haiti was assassinated, we've got the Cuba stuff going on, we got all of this madness around us. And the way that I always see have seen it is when the madness is happening around me, the only way for me to be the eye is for me to go inside. And outwardly focus from within my energy so that I'm pushing at the hurricane, so to speak versus and I'm in the eye instead of being in in the storm. But and obviously that works. Sometimes it doesn't work others, that's just the visual that I have. But we were talking about this, like, how does somebody get out of this place of madness that they're in? Whether it's web site, I don't care if the political or religious or scientific spectrum or cultural spectrum? It's everywhere right now. It's like, it's like a furnace has been lit. And and it's building pressure, right? I think something like we're in a pressure cooker. Yeah, let's talk about how do we let the steam out of the pressure cooker a little bit and then pop the top. So we're not in it? And do that in a safe way. But, you know, like, how do we get to that place from where we're at? Because what you're talking about feels very idealistic. I want to take it out of the idealism and into realism into how can somebody how can we do this? Now? How can we be in this?Gunther Mueller17:45So the idea, let's take the analogy of the pressure cooker. What if you do not have to reduce the pressure? But what if you can exist within the pressure and not be affected by the pressure?Ari Gronich18:04I guess that that's how I feel within like that I have a hurricane. Right?Gunther Mueller18:10It's a great visual, it is a great visual because there is infinite calm in the eye of the hurricane. To the left, there's chaos to the right, there's chaos, stuff blowing up, getting knocked down over here, stuff blowing up and knocked out over there. But in the middle, no wind, no storm in the eye could even be sunny in the middle of the hurricane. You know, it's like this whole Sun comes through and beautiful day. But the Hurricanes moving right. So the idealism, it only seems ideal, because it's a new concept. And just as asked was a new concept, you know, 30 years ago, that kind of thing, right? And rebirthing, we talked about that offline to so many techniques and things like that, to what to help us feel better. That's really what the human experience is, we want to just feel better. We want, we want what we want, which is to sum it up, less pain, more satisfaction, we want less pain and more satisfaction, you can throw the words meaning fulfillment in there. And what we talked about offline briefly was this pressure cooker feeling is like I described as people I think are getting to the point globally. Now. You mentioned all the places where there's unrest and problems going on. They are tired. They've had enough of not having enough. And I've always thought this look when you have nothing to lose. You have nothing to lose. And so you're going for it all because the current situation is not worth maintaining anymore. There's nothing in it anymore. It's painful, is gotten to the point where the pain of that existence. It is time to do something about it. But again, if you look at the world, they're solving it from the problem-solving real reality, we need to overthrow the dictator, we need to get a new government, we need to be left or right, we need to do this we the problem solving is there. So to answer your question that you asked me earlier a little bit, I wanted to inject the idea of we need to be it in order to see it. And the personal development movement have had has had that switched around a little bit, that as we start seeing results, we can be more that of that thing, right? I get when I have a billion dollars, I can be generous, right? So I need to create all this stuff. I gotta be a billionaire. And then I'll be able to, you know, be generous, like, if you're not generous now, in the current situation, you will not be generous. how many billionaires Do you know, I don't know that many of them. But I've heard of, and I read their stories, right? They're in fear of losing what it is they have. They don't have the bliss and the peacefulness and the calm in their life, and the experience that most of us really want or the freedom, okay, and we think that Oh, being that person like, the responsibility that comes with that position, the number of people that are trying to take your stuff, when you're in that position, the attacks that are coming at you, we think, oh, because, you know, we're in our secure Oh, it'd be so much better to be that guy. I'm here to tell you, not really not unless you structure it properly with the end in mind. Now, there are some people that have that, let's say kind of wealth, and I talk about wealth, not because it's the most important thing, because it's on a lot of people's minds. It's easy to measure, right? And when you look at the world, that seems to be what the irritation is, is not having enough. Ari Gronich21:53Let's say, you know, we go to the statistic 1.87, I believe trillion dollars into the like, top 10, 20 people in the world, their wealth over the course of COVID. Right. Whereas we spent, I think it's around 3 trillion. So I'm just going to correlate it right. So I correlate it, like the government spent 3 trillion of taxpayers money, 2 trillion of that approximately went into 20 people's hands. Right. So there's a correlation between wanting, I guess, fairness or equanimity and these kinds of things within the situation that that we aren't seeing, right. So if we're not seeing the fairness and equanimity that pain level goes up, as you were saying, and then the pressure cooker arises. But I don't think that people correlate the two things like they don't say, two, or 3 trillion came out of people's hands and into 20 people's hands, like out of a few 100 million into 20 people's hands. They don't say that they don't, they just say during this period of time, these top 20 people, their wealth skyrocketed, and these people their wealth went, right. So if we don't get the correlation, how do we get to the end, I'm going to use the word solution but as a problem solving, but how do we get to that place where equanimity fairness, those things, where as they're not guaranteed in life, are at least structured more appropriately or so that people can have the sense that when they do something like this magnetic mind, you know, and they're doing these five steps that they actually think that that true choice can happen?Gunther Mueller24:03Yeah, so anything high Einstein said this to write anything that you can imagine, you can create, anything that's ever been created started in the imagination first, but you have to think of your life in little bubbles, you are in this little bubble right here. Okay. And that's just you, your desires, your true choices, the experience that you want in the current reality, and you want this experience just because you'd love it, just because you want it just because that's the experience you want to have. It does not mean that the entire world has to change for you to have this experience in your life. And let's just stick with the wealth or abundance type thing. In order for you to have the experience of abundance. It doesn't mean you have to be one of the 20 people. Ari, I want to use an example of breathing The last time you thought about how much air was available to you today to breathe.Ari Gronich25:06I'm a weird one, I think about it because I think about cleanliness and the air. But you know. Gunther MuellerThat's different from quantity, right? Ari GronichThat is different. quality versus quantity. Yeah, that's different.Gunther Mueller25:17But our experience as human beings is that we've always had pretty much unless you're drowning, or you're locked in a sealed box or something like that we've had an infinite air supply, we can breathe as much as we want as fast as we want. We've never really thought about, you know, is there going to be enough air today for me to survive? Know the so even in the current reality in the current moment, if you focus on just breathing, you can have the experience of abundance. That's what abundance feels like having more than enough. And so let's say in our lives, if we want to create that experience of abundance, if you have $10, left over from your budget, at the end of the month, you have more than you needed, you just have $5 left over at the end of the month. That is an experience of abundance, it may not match your desire. But this is what creates the contentment in the moment just for a time since you can plant your feet. And you can be it now. Okay, you can be it now you can experience abundance of what it feels like to be abundant in your little bubble. All right now around this bubble, is your family, friends and influence your little tribe is around there. And these are the ones that could be speaking, some sort of negativity into your thing, right, but you're in this bubble, you have a true choice, you have a desire, you have the thing that you would just love to experience for no other reason than the fact that you want it, you love it. And this field here is either going to you're going to influence this field or this field is going to influence you. And the more you secure yourself in your own being listening to your own voice, your own desires, and you focusing on that which you want, and not trying to solve for world peace or trying solve all the ills and all the problems in the world. It's the analogy of the airplane, right, you have to put your oxygen mask on first, before you can help anyone else. So getting in to the conscious creator stance, and choosing that which you want, creates this little bubble. And you can experience that which you choose to experience in this little bubble and it does work. Okay, the magnetic mind method has even restored eyesight, we're not promising that but we had a blind person go through a series of recodes and restored the eyesight because the identity shifted from a person that did not see to a person that now sees, we've had people get out of wheelchairs, because the identity has shifted, again, extreme examples of what is not promising that everybody, but when the identity shifts, the current reality changes. And that reality includes the bubble of your family may not like how your family and friends treat you or done it enough. But that can change too. When this changes, then this changes when this bigger bubble then changes, then the outer bubble changes and the more people that are taking this responsibility for themselves and manifesting their own true choice experience. And imagine if more and more and more people did this on a regular basis. And I regular by i mean you know, once or twice a day is getting into that field and being clear about what you want. Because you have to send that vibration into the field superconscious needs to know that you're serious about what you want. It can't just

Stop looking at me Schwann.

Stop looking at me Schwann.