Podcasts about adjuvants

Vaccines have become one of the most polarizing topics in public health, and frankly, for valid reasons. What we've witnessed over the past few years is an unprecedented breakdown in trust between medical institutions and the public - and that didn't happen in a vacuum.What is the truth about Vaccine Safety and Efficacy? Dr. Roger McFillin sits down with vaccine safety epidemiologist Allison Krug to expose the hidden forces shaping public health, from vaccine safety to the weaponization of fear. They dive deep into the failures of placebo-controlled trials, the truth about natural immunity vs. vaccine-induced immunity, and why good evidence is harder to find than you think. Allison shares what she learned working inside the pharmaceutical industry and reveals how mindset, belief systems, and disconnection from nature and spirituality are fueling today's chronic illness crisis. If you've ever questioned mainstream medicine or want to reclaim your health and autonomy, this conversation will change how you see the world.00:00 Introduction00:05:32 The Danger of Outsourcing Health to "Experts"00:20:45 Mindset, Pain, and the Illusion of Powerlessness00:42:49 Consciousness and the Healing Power Within01:05:32 Germ Theory, Genetics, and the Loss of Agency01:15:47 Awakening to Frequencies: A Spiritual Revolution01:22:23 Dark Spiritual Forces and the Battle for Consciousness01:31:27 How Public Schools Crush Creativity and Independence01:49:05 Medical Paradigm Flaws: What They're Not Telling You01:55:40 Spiritual Emptiness: The Hidden Cost of Modern Culture02:00:12 Radical Responsibility and the Return to Divine LoveAllision Krug WebsiteAllision Krug on XAllison Krug on Megyn Kelly COVID-19 vaccine boosters for young adults: a risk benefit assessment and ethical analysis of mandate policies at universities  Dr. Roger McFillin / Radically Genuine WebsiteYouTube @RadicallyGenuineDr. Roger McFillin (@DrMcFillin) / XSubstack | Radically Genuine | Dr. Roger McFillinInstagram @radicallygenuineContact Radically GenuineConscious Clinician CollectivePLEASE SUPPORT OUR PARTNERS15% Off Pure Spectrum CBD (Code: RadicallyGenuine)10% off Lovetuner click here

In this episode, we are joined by a friend and repeat guest, Paul Crout. Paul is a leading pest control advisor, certified crop control advisor, and one of the foremost experts on wine grape nutrition, spray adjuvants, and more in the ag and viticulture realm. He currently works as a leading agronomist with Helena Agra Enterprises in California, and he has the honor of being the only guest we've had on the podcast three times! Adjuvants can be a dry topic, but we promise this discussion provides a lot of valuable take-home information. It's one of those episodes where you will want to take notes. Listen in and don't forget to download the Spray Adjuvants to Increase Pesticide Efficacy Grower Guide. In this episode, you will hear: What adjuvants are, when to use them, and which ones to use The two main categories of adjuvants - surfactants and special purpose When to use buffers and drift control agents The importance of knowing your water's pH before adding an adjuvant Differences between non-ionic surfactants and organosilicone surfactants Understanding the use of methylated seed oils for tank mixing and pesticide application Follow and Review: We'd love for you to follow us if you haven't yet. Click the ‘+ Follow' button in the top right corner of your Apple Podcasts app. We'd love it even more if you could drop a review or 5-star rating over on Apple Podcasts. Simply select “Ratings and Reviews” and “Write a Review,” then a quick line with your favorite part of the episode. It only takes a second, and it helps spread the word about the podcast. Resources: Download Fritz's member-only Spray Adjuvants to Increase Pesticide Efficacy Grower Guide available to podcast listeners through August 2025. If you are starting a vineyard or thinking about starting a vineyard, apply to be on the podcast for Fritz's help planning your vineyard - https://www.vineyardundergroundpodcast.com/contact/ Come grow with Fritz through VirtualViticultureAcademy.com! Save $75 off your first year of membership with the code Underground at checkout.  Today's Guest: Paul Crout is a Pest Control Advisor and Certified Crop Advisor, and one of the foremost experts on wine grape nutrition and spray adjuvants. He currently works as an Agronomist with Helena Agri-Enterprises in California. Connect with Paul on LinkedIn and learn more about being Grape Wise with Helena. Episode Sponsor: Today's episode was brought to you by SensorInsight and by Vigneron Toy Store. Thank you to SensorInsight for sponsoring today's episode. SensorInsight is a leader in vineyard soil moisture and weather monitoring. SensorInsight designs state-of-the-art, remote satellite monitoring of soil moisture and weather, bringing actionable information for your vineyard operations straight to your phone, with real-time data so you can best decide when to turn on your irrigation, how long to run the system, and learn how the dynamics of soil moisture can change over a given season. Visit them today at https://sensorinsight.io. And don't forget to mention the code Underground to receive a discount on your SensorInsight solution. Thank you to Vigneron Toy Store for sponsoring today's episode. Vigneron Toy Store specializes in Harvesters, Sprayers, Frost Mitigation Equipment, Canopy Management Trimmers, and more. As grape growers themselves, they can expertly help you determine the best equipment for your specific vineyard needs. And, as a special offer for our podcast listeners, you can save 5% off in-stock machines by entering the promo code Underground on the contact form at vignerontoystore.com. Episode Credits If you like this podcast and are thinking of creating your own, consider talking to my producer, Emerald City Productions. They helped me grow and produce the podcast you are listening to right now. Find out more at https://emeraldcitypro.com. Let them know we sent you.

AABP Executive Director Dr. Fred Gingrich is joined by Dr. Chris Chase, Emeritus Professor in the Department of Veterinary and Biomedical Sciences and Dr. Curt Vlietstra, Senior Professional Services Veterinarian with Boehringer Ingelheim. This episode is sponsored by Boehringer Ingelheim, manufacturer of the Pyramid line of cattle vaccines. Find more information about Pyramid/Presponse vaccines by visiting this page.  Adjuvants are a critical component of vaccines, but they can be an overlooked factor when putting together a vaccine program. The three roles of adjuvants serve in vaccines are creating a danger signal for the host, help the immune system recognize the antigen and to protect the antigen.  Historically, there has been some skepticism around vaccinating young calves early due to the presence of maternal antibodies. Our guests discuss what role vaccines serve in a calf program while recognizing the importance of colostral immunity and the purpose it serves. Adjuvants can serve an important role in young calves to help with a vaccine-induced immune response. Our guests discuss the METASTIM adjuvant, how it differs from other adjuvants, and the role it plays in boosting immunity even in the face of maternal antibodies.  Veterinarians play a critical role in developing vaccine programs for their clients' herds. This not only includes ensuring that the appropriate vaccines are selected for the herd, but also making sure that nutrition, specifically vitamin and mineral nutrition, is appropriate, stress is minimized, and vaccine storage and administration is correct. We close the podcast by discussing the future of vaccine and adjuvant technologies that veterinarians should be aware of and how these potential uses can improve cattle health. Veterinarians can go to https://bi-animalhealth.com/cattle/ or talk to their Boehringer Professional Services Veterinarian for more information.

Ashley Everly is a Toxicologist and the founder of Vaccine.Guide, a one-stop resource on research and information regarding vaccines and the infectious diseases they aim to prevent. In this conversation with Ashely we discuss:The adverse event that led her to investigate vaccinesDiscovering that there is no "happy medium"The woefully inadequate safety studies done on vaccinesThe issue(s) with aluminum adjuvantsOther problematic ingredients...and more!Learn more about Ashley and her work at https://vaccine.guide/ or at https://everlyreport.com/.Support Terrain Theory on Patreon! Our recently-launched member platform gives you access to a ton of free & exclusive content. Check it out: https://www.patreon.com/TerrainTheoryTerrain Theory episodes are not to be taken as medical advice. You are your own primary healthcare provider.If you have a Terrain Transformation story you would like to share, email us at ben@terraintheory.net.Learn more at www.terraintheory.netMusic by Chris Merenda

MustGrow Biologics Corp Chief Executive Officer Corey Giasson joined Steve Darling from Proactive to announce a major milestone for the company: the signing of a five-year exclusive distribution agreement with Adjuvants Plus, a leading Canadian regenerative agriculture company. Under this agreement, MustGrow will distribute Adjuvants Plus' product line across Canada through its recently acquired sales and distribution division, NexusBioAg. Additionally, MustGrow has secured a First Right of Refusal for potential U.S. market distribution, positioning the company for further expansion into North America's agricultural sector. This deal represents the first additional product line added to NexusBioAg's portfolio, reinforcing MustGrow's commitment to bringing innovative and sustainable agricultural solutions to North American farmers. The partnership aligns with both companies' shared vision of advancing regenerative agriculture—enhancing crop productivity while preserving soil health for future generations. The agreement covers four key products, with a particular emphasis on EndoFine® and EndoGuard®, which provide significant benefits for various North American crops, including Corn, Soybean, Canola and Cereals. The demand for sustainable agricultural solutions continues to grow, driven by increasing production of major Canadian crops. According to Statistics Canada, planting projections include Wheat rising to 27 million acres while Canola is expected to reach 21.4 million acres. With this strategic agreement, MustGrow is well-positioned to expand its footprint in regenerative agriculture, offering farmers innovative, eco-friendly solutions to improve yields while maintaining long-term soil health. #proactiveinvestors #mustgrowbiologicscorp #tsxv #mgro #otcqb #mgrof #mustardseed #TerraSante #Biofertility #Agriculture #BiologicalFarming #RegenerativeAg #SustainableFarming #NexusBioAg #AgTech #CropProtection #FarmingInnovation #CanadianFarming #adjuvantsplus

To set your fields up for success in 2025, it's important to have a fall burndown program in place to eliminate weeds and other unwanted growth from your fields. Helena Brand Manager Austin Anderson provides his top three products to include in a fall burndown program to maximize the performance of herbicides. (00:40)  Later, Jody Lawrence provides an update on the newest commodity report. (12:48) Follow Helena Agri on social media to stay informed:  Facebook: https://bit.ly/3pq8XVJ Instagram: https://bit.ly/347QAO8 X: https://bit.ly/3hwvWdG LinkedIn: https://bit.ly/3pwWLTh YouTube: https://bit.ly/35pLLQJ

Have you started to wonder why food allergies are on the rise? Why do so many now suffer from autoimmune diseases? On this important episode of Vitality Radio Jared invites Gerald Roliz, CNC, author of The Pharmaceutical Myth, to join him in a discussion about one of the most prevalent toxins we are exposed to - vaccines. You'll learn how the body's immune system responds to a vaccine and why this can lead to food allergies and autoimmune disease. It might surprise you to learn that the most common food sensitivities are also ingredients in vaccines! You'll also learn how the body's innate healing ability can be restored through detoxification and rebuilding of the body through nutrition.Gerald is a former pharmaceutical sales rep turned Functional Nutrition Consultant and is helping to reverse the damage from pharma and other environmental toxins, including vaccine injury. Gerald started out selling medicine but realized he was not seeing people get well. His realization that medicine was suppressing symptoms, not getting people healthy, led him to nutrition. Gerald is the lead clinician at the Natural Health Improvement Center in Idaho.  He also teaches natural health clinicians on the implementation of Functional Nutrition and Foundational Medicine across the country.Additional Information:The Pharmaceutical MythLearn more about vaccine adjuvants#272: Turtles All The Way Down, Science and Myth with Zoey O'Toole and Mary Holland#270: Del Bigtree: Whose Interests are American Health Agencies Really Protecting?#181 VR Special: Understanding Informed Consent and Vaccinations with Dr. Paul ThomasVisit the podcast website here: VitalityRadio.comYou can follow @vitalityradio and @vitalitynutritionbountiful on Instagram, or Vitality Radio and Vitality Nutrition on Facebook. Join us also in the Vitality Radio Podcast Listener Community on Facebook. Shop the products that Jared mentions at vitalitynutrition.com. Let us know your thoughts about this episode using the hashtag #vitalityradio and please rate and review us on Apple Podcasts. Thank you!Please also join us on the Dearly Discarded Podcast with Jared St. Clair.Just a reminder that this podcast is for educational purposes only. The FDA has not evaluated the podcast. The information is not intended to diagnose, treat, cure, or prevent any disease. The advice given is not intended to replace the advice of your medical professional.

Support the Podcast: https://www.insidethehive.tv/offers/rozVB5Y2 Sign up to my email list: https://www.insidethehive.tv/newsletters Brandon's publication: https://academic.oup.com/jinsectscience/article/23/6/20/7458958 Vita Bee Health: https://www.vita-europe.com/beehealth/ _______________________________________________________________ In today's episode, I had the pleasure to speak with Dr. Brendan Shannon, a PhD student at Ohio State University and an expert on pesticides and adjuvants. Dr. Shannon's work has been crucial in understanding the impact of these chemicals on the health of honeybees. His most recent work is a review article about adjuvants and their effects. In this interview, recorded live with members of my academy, we discuss the pros and cons of pesticide and adjuvant combinations, the surprising ways researchers and farmers are using this knowledge to mitigate negative impacts on honeybees, and other topics.

In this episode of RAPM Focus, Dr. Brian Sites is delighted to support the work of Dr. Ottokar Stundner, MD, and colleagues by discussing the use of liposomal bupivacaine trends following the March 2024 publication of “Crystallization of mixtures of local anesthetics with and without select adjuvants: a semiquantitative light microscopy analysis.” Dr. Studener completed his anesthesia residency in Salzburg, Austria, as well as a research fellowship in regional anesthesia and perioperative outcomes at the Hospital for Special Surgery in New York. After his training, he joined the faculty at Innsbruck Medical University, also in Austria, where he currently is co -chair of pediatric anesthesia. He holds an MBA in Master of Public Health degrees. from the Imperial College of London and is an editorial fellow at the BJA. With its high expense and questionable benefit over conventional forms of local analgesia, it is possible that the use of liposomal bupivacaine is declining—especially in the United States. *The purpose of this podcast is to educate and to inform. The content of this podcast does not constitute medical advice, and it is not intended to function as a substitute for a healthcare practitioner's judgement, patient care, or treatment. The views expressed by contributors are those of the speakers. BMJ does not endorse any views or recommendations discussed or expressed on this podcast. Listeners should also be aware that professionals in the field may have different opinions. By listening to this podcast, listeners agree not to use its content as the basis for their own medical treatment or for the medical treatment of others. Podcast and music produced by Dan Langa. Find us on X @RAPMOnline, Facebook @Regional Anesthesia & Pain Medicine, and Instagram @RAPM_Online.

Choosing the right adjuvants can make or break a tank mix. Codee Lee, a crop protection and adjuvants expert with CHS, offers insights about adjuvant selection to complement crop protection products and maximize yield potential.

Choosing the right adjuvants can make or break a tank mix. Codee Lee, a crop protection and adjuvants expert with CHS, offers insights about adjuvant selection to complement crop protection products and maximize yield potential.

Spring is officially here, with the first annual weeds starting to grow. Farmers are preparing for the planting season, with some already starting to seed their fields. The weather is predicted to be dry and warm, which will impact the tillage practices. Adjuvants will be crucial for effective burndown of weeds, especially with the dusty conditions. Crop choices for the season include lentils, chickpeas, barley, canola, and soybeans. Sunflower and quinoa cultivation is limited due to market prices and weed challenges. Faba beans are a good crop option for northern regions. The conversation covers various topics related to agriculture, including the challenges of growing faba beans, the emergence of specialty crops like safflower and buckwheat, the importance of early preparation for the spring season, and the potential pest and disease issues that farmers may face. Jason and Kyle also discuss their appreciation for different types of beers, including hazy IPAs and lagers.

Join our host, Mike Howell as he chats with Dr. Karl Wyant, Director of Agronomy at Nutrien about mixing liquid fertilizers. They'll get into jar testing, the benefits, possible risks, protecting yourself and your crops and who to go to when you have questions.   To discover the latest crop nutrition research, visit nutrien-eKonomics.com.

In this episode, Cody Lee, a technical specialist for CHS, joins the hosts to discuss the importance of adjuvants in agriculture. Everyone shares their personal experiences and stories from the field, highlighting the role of adjuvants in weed control and the challenges faced in different environmental conditions. They emphasize the need for personalized recommendations based on specific weed spectra and environmental factors. The conversation also touches on the impact of dry years on adjuvant performance and the importance of building personal relationships in the industry. The episode concludes with a discussion on the value of gaining experience and learning from mistakes. This part of the conversation focuses on the use of adjuvants in herbicide mixes and the challenges and considerations involved in selecting the right adjuvant. The complexity of the glyphosate market and the impact of adjuvants on weed control are also discussed. The economic value of adjuvants and the importance of proper stewardship practices are highlighted. The chapter concludes with a discussion on the use of adjuvants for specific crops and weeds. In this conversation, the speakers discuss various aspects of adjuvants in agriculture. They talk about the different adjuvants used in the past and how the industry has evolved. They also share their favorite adjuvants and discuss the importance of humectancy in different environments. They emphasize the role of stewardship in adjuvant selection and highlight the value of practical experience. They also touch on unrelated topics such as the success of Velva's football program and the popularity of Dot's Pretzels. The conversation concludes with a discussion on the importance of learning from others and seeking advice from technical specialists.

OB/GYN Dr. Julio Novoa, who not only provides excellence in women's health care but is renowned for his minimally invasive techniques, joins the HMP to discuss all things foreign body reactions, metal hypersensitivity, ASIA syndrome and beyond. Not only a OB/GYN, Dr. Novoa also specializes in cosmetic gynecology but might just shine as the brightest light in his concern for women's safety and advocacy, who agrees more with a patient-centric model for healthcare as opposed to approaching medicine strictly from a business perspective.  Seeing a trend towards unnecessary medical procedures and not being a fan, he became alarmed with routine practices in surgical standards that harmed the patient. Noticing a large subset of patients with severe symptoms from foreign body implantation, such as the Essure permanent sterilization device, surgical clips and staples, leaky breast implants and more, he knew he had to speak out and continues to do so today.  We discuss a newer-term and evolving diagnoses: ASIA Syndrome or Autoimmune Inflammatory Syndrome Induced by Adjuvants. Dr. Novoa explains what an adjuvant is and why it matters. We discuss various types of foreign body reactions and why surgeons should be able to readily explant anything they are keen to implant. We also discuss localized and systemic hypersensitivity reactions and whether or not everyone who has them can be classified under the definition of the ASIA effect.  Dr. Novoa goes on to detail clinical observations and personally observed results from the large amount of explant surgeries he has performed and witnessed. More and more complete resolution of the patient's vast symptoms upon explantation became the rule, rather than the exception and he shares some of those experiences. He states his opinion on whether asymptomatic patients with implants should consider having them removed after healing has occurred. A lengthy discussion ensues surrounding how to affect change within the medical communities, physicians groups and governing bodies and Dr. Novoa offers his opinion as to why physicians aren't putting two and two together and completely missing the boat when patients present with systemic and localized foreign body reactions. We also go into the super-serious topics surrounding informed consent and how we need to up our game with our own surgical consents and medical records. This episode will encourage patients to look more closely prior to surgery and to find their voice when experiencing practices that could increase the risk of foreign body reactions.  You'll also want to tune in to hear who is Dr. Novoa's favorite heavy metal band!  In this episode you will learn: All about informed consent.  Why keeping copies of op and pathology reports is critical.  How to affect change in medicine.  Can what's put in come out? ~Links and Resources~ ~ Check out Dr. Novoa & The Novoa Medical Services website HERE.    ~ ~To learn more visit ⁠HeavilyMetalled.com⁠. ~Sign the petition to urge metal hypersensitivity screening HERE.  ~ Check out the “Heavily Metalled” resource page: ⁠https://www.heavilymetalled.com/resources⁠ ~ Follow Heavily Metalled on Facebook: ⁠https://www.facebook.com/HeavilyMetalled⁠ ~ Follow Heavily Metalled on Instagram: ⁠https://www.instagram.com/heavilymetalled/⁠ ~ Subscribe to Heavily Metalled on YouTube: ⁠https://www.youtube.com/@HeavilyMetalled⁠ ~ Check out our sponsor ⁠https://melisa.org⁠ ***Many WONDERFUL, supportive special-interest communities exist for metal allergies and diet, hardware issues, medical devices, etc., online and on social media. They have many resources and often act as a collective think-tank. I owe many parts of my recovery to knowledge obtained in such groups. Search keywords to join these groups and find your tribe!***

Hosts Cameron Horine and Adam Jones discuss crop protection and adjuvants with MFA's Sr. Director of Seed & Crop Protection Jordan Tomlinson, and Crop Protection Product Specialist Taylor Dennigmann. Jordan gives an industry outlook on product availability and price trends. Taylor specializes in adjuvants and micronutrients. She recently created a short explainer video that goes over the difference between types of adjuvants and just how to best use them. There is also a printed guide available for download on MFA's Crop Protection page. Check out the podcast at the links below, also now available on iHeart Radio. Note: the Apple Podcast link is free of interruption from outside ads. Issue 55 Adjuvants and a 2024 crop protection supply outlook. The Made for Agriculture podcast

Charlotte Barker, Editor, Vaccine Insights, speaks with Ed Lavelle, Professor, Trinity College Dublin, about our growing understanding of adjuvant mechanisms, his work on developing mucosal vaccines, and why size matters for nanoparticles' adjuvant effects.

Welcome to The Nonlinear Library, where we use Text-to-Speech software to convert the best writing from the Rationalist and EA communities into audio. This is: The literature on aluminum adjuvants is very suspicious. Small IQ tax is plausible - can any experts help me estimate it?, published by mikes on July 4, 2023 on LessWrong. I am a PhD biostatistician who has worked on a project in this area. I am hoping to crowdsource opinions on this issue, especially from readers with knowledge of neurology, nephrology and/or toxicology.The recent news cycle motivated me to spend some time reading up on the safety literature for aluminum vaccines. Aluminum is not in the Covid vaccine, but it is in most childhood vaccines.I am deeply unsettled by what I found. Here is a summary. I would appreciate being corrected in the comments:- The science on this topic is surprisingly neglected. Aluminum is a known neurotoxin in small concentrations in the brain. So, the obvious follow-up question: "Is the amount accumulated from childhood vaccines so small as to be irrelevant?" Unfortunately, aluminum has not undergone rigorous safety testing for cumulative cognitive effects. The fact that I could not find a convincing answer is a multi-decade policy failure.-The evidence for safety cited by regulators and mainstream review papers appears to have gaping issues (see end of post for notes on the studies Mitkus et al (2011) and Karwowski et al (2018). They do not justify anywhere near the faith being placed in them.)-Mainstream educational sources are verifiably gaslighting readers by suggesting that the net exposure in vaccines is less than dietary exposure; but this fails sanity checks with basic multiplication, given the oral bioavailability of aluminum is about 0.1% on average in diet and 4 mg in the whole schedule). That's my rough fermi estimate, based on an experiment showing developmental delay in premature infants exposed to hundreds of micrograms aluminum in IV fluids (Bishop et al 1997). This estimate is extremely sensitive to assumptions about renal clearance and sensitivity of brain development in premature infants compared to full term. Can anyone suggest a more accurate method? With this approach: does anyone know enough nephrology to pin down the length of time that intravenous aluminum in Bishop's premies would have spent circulating in blood, given their reduced renal function? Anyone know enough neuro to comment on whether impacts on cognitive development should be linear in the amount of neurotoxin? How much larger should it be in premies vs term infants?______Notes on failures in the literature:Claims of safety of aluminum seem to bottom out in mainly two papers: Karwowski et al (2018) and Mitkus et al (2011). The FDA cites Mitkus 2011 to determine a tolerable risk level of aluminum exposure and argues that vaccine exposure is below it; the UK health authority and various review papers (such as this one) cite Karwowski as evidence of low accumulation of aluminum in infants. Study 1 Mitkus et al (2011) which the FDA commissioned on this topic, argues that exposure to aluminum due to vacc...

Link to original articleWelcome to The Nonlinear Library, where we use Text-to-Speech software to convert the best writing from the Rationalist and EA communities into audio. This is: The literature on aluminum adjuvants is very suspicious. Small IQ tax is plausible - can any experts help me estimate it?, published by mikes on July 4, 2023 on LessWrong. I am a PhD biostatistician who has worked on a project in this area. I am hoping to crowdsource opinions on this issue, especially from readers with knowledge of neurology, nephrology and/or toxicology.The recent news cycle motivated me to spend some time reading up on the safety literature for aluminum vaccines. Aluminum is not in the Covid vaccine, but it is in most childhood vaccines.I am deeply unsettled by what I found. Here is a summary. I would appreciate being corrected in the comments:- The science on this topic is surprisingly neglected. Aluminum is a known neurotoxin in small concentrations in the brain. So, the obvious follow-up question: "Is the amount accumulated from childhood vaccines so small as to be irrelevant?" Unfortunately, aluminum has not undergone rigorous safety testing for cumulative cognitive effects. The fact that I could not find a convincing answer is a multi-decade policy failure.-The evidence for safety cited by regulators and mainstream review papers appears to have gaping issues (see end of post for notes on the studies Mitkus et al (2011) and Karwowski et al (2018). They do not justify anywhere near the faith being placed in them.)-Mainstream educational sources are verifiably gaslighting readers by suggesting that the net exposure in vaccines is less than dietary exposure; but this fails sanity checks with basic multiplication, given the oral bioavailability of aluminum is about 0.1% on average in diet and 4 mg in the whole schedule). That's my rough fermi estimate, based on an experiment showing developmental delay in premature infants exposed to hundreds of micrograms aluminum in IV fluids (Bishop et al 1997). This estimate is extremely sensitive to assumptions about renal clearance and sensitivity of brain development in premature infants compared to full term. Can anyone suggest a more accurate method? With this approach: does anyone know enough nephrology to pin down the length of time that intravenous aluminum in Bishop's premies would have spent circulating in blood, given their reduced renal function? Anyone know enough neuro to comment on whether impacts on cognitive development should be linear in the amount of neurotoxin? How much larger should it be in premies vs term infants?______Notes on failures in the literature:Claims of safety of aluminum seem to bottom out in mainly two papers: Karwowski et al (2018) and Mitkus et al (2011). The FDA cites Mitkus 2011 to determine a tolerable risk level of aluminum exposure and argues that vaccine exposure is below it; the UK health authority and various review papers (such as this one) cite Karwowski as evidence of low accumulation of aluminum in infants. Study 1 Mitkus et al (2011) which the FDA commissioned on this topic, argues that exposure to aluminum due to vacc...

*****Watch on Rumble: https://rumble.com/v2xdg7m-bret-and-heather-180th-darkhorse-podcast-livestream.html*****In this 180th in a series of live discussions with Bret Weinstein and Heather Heying (both PhDs in Biology), we discuss the state of the world through an evolutionary lens.We begin by discussing Bret's 2021 conversation with RFK Jr., which DarkHorse released this week—why the delay, and what revelations are therein? Then we discuss adjuvants in killed virus vaccines, and whether the intentional awakening of the immune system with adjuvants may contribute to allergies. And we discuss affirmative action, in the wake of the Supreme Court's decision this week, which found in a 6 – 3 decision that using race as a factor in college admissions is unlawful. Reviewing some of the relevant legal history—the ratification of the 14th Amendment (1868), Plessy v Ferguson (1896), Brown v Board of Education (1954), University of California v Bakke (1978), and Grutter v Bollinger (2003)—we then discuss the opinions put forth by both the majority and dissent in this case.*****Our sponsors:Biom: NOBS is a different, superior kind of toothpaste. Try it, you'll never go back. Go to www.betterbiom.com/darkhorse to get 15% off your first month supply, until July 17, 2023.American Hartford Gold: Get up to $5,000 of free silver on your first qualifying order. Call 866-828-1117 or text “DARKHORSE” to 998899.Seed: Start a new healthy habit today with Seed probiotics. Visit https://seed.com/darkhorse, and use code darkhorse, to get 25% off your first month of Seed's DS-01® Daily Synbiotic.*****Our book, A Hunter-Gatherer's Guide to the 21st Century, is available everywhere books are sold, including from Amazon: https://a.co/d/dunx3atCheck out our store! Epic tabby, digital book burning, saddle up the dire wolves, and more: https://darkhorsestore.orgHeather's newsletter, Natural Selections (subscribe to get free weekly essays in your inbox): https://naturalselections.substack.comFind more from us on Bret's website (https://bretweinstein.net) or Heather's website (http://heatherheying.com).Become a member of the DarkHorse LiveStreams, and get access to an additional Q&A livestream every month. Join at Heather's Patreon.Like this content? Subscribe to the channel, like this video, follow us on twitter (@BretWeinstein, @HeatherEHeying), and consider helping us out by contributing to either of our Patreons or Bret's Paypal.Looking for clips from #DarkHorseLivestreams? Check out our other channel:  https://www.youtube.com/channel/UCAWCKUrmvK5F_ynBY_CMlIATheme Music: Thank you to Martin Molin of Wintergatan for providing us the rights to use their excellent music.*****Q&A Link: https://rumble.com/v2xdh70-your-questions-answered-bret-and-heather-180th-darkhorse-podcast-livestream.htmlMentioned in this episode:Supreme Court Decision in Students for Fair Admissions v Harvard / UNC, decided June 29, 2023: https://int.nyt.com/data/documenttools/supreme-court-decision-on-race-based-admissions/0a725aaabb459074/full.pdfUC Regents v Bakke: https://www.law.cornell.edu/wex/regents_of_the_university_of_california_v_bakke_(1978)Grutter v Bollinger: https://www.law.cornell.edu/wex/grutter_v_bollinger_(2003)Support the show

An immune boosting vaccine substance developed by two Wellington academics is now being commercialised. Victoria University Associate Professors Mattie Timmer and Bridget Stocker made the initial discovery five years ago, and have been developing the ingredient, known as an adjuvant. Adjuvants are added to some vaccines to improve their efficacy and reduce side-effects. Now, along with Victoria University's commercialisation office - Wellington UniVentures, the academics have licensed some of their adjuvants to a US biotech company that will see them used in human clinical trials across a wide range of diseases. They're also preparing to spin-out a new company that will produce adjuvants and sell them onto companies that make vaccines for animals.

Redstar and Agronomy 2024 ConversationBio~Ryan & Kristin Mullenhoff / OwnersRyan and Kristin were born and raised on farms near Leigh, NE, in 2004, they started a small seed corn and soybean business. They welcomed their only child, a son Jackson Charles in May of 2005. Over the next couple of years, they quickly found this "short-term" opportunity to become their forever future. In 2010, they added fertilizer and a list of other services prompting them to change their name to Ryan Mullenhoff Sales, LLC. Over the next couple years their fertilizer sales thrived and in 2018 they developed and branded their own line of low-salt starters called REDSTAR Fertilizer. They expanded in to other states. To better reflect their company, Ryan and Kristin officially changed their name to REDSTAR, LLC in 2020. After realizing the success of REDSTAR Fertilizer, Ryan and Kristin expanded their branded line to include Biologicals, Seed Treatments, Amino Acids, Adjuvants and more.In 2023 they introduced a third division of REDSTAR called REDSTAR Branded.  Main Talking PointsThe Early Days of REDSTAR (formally Ryan Mullenhoff Seeds)How did you initially get startedWhat were some of the biggest challengesWhat did your operation and team look like back in those daysREDSTAR FERTILIZERWhere did the idea of low-salt starter fertilizer come from? Did you solve a problem?How did adding fertilizer affect your businessWhat made you decide to build and produce your own line of branded fertilizerWhat makes your fertilizer uniqueCompany GrowthWhat kind of growth has your company seen, and what challenges or opportunities has it brought to your businessFor listeners looking to grow their farm or business what do you have for advice?Let's Talk about the REDWhy REDSTAR, why the RED FertilizerWhat makes RED so much better and what's behind all the REDWhat make REDSTAR Different than other Ag DealershipsWhat are some things that you do that set REDSTAR ApartRyan and KristinTell me what it's been like working together for the last 19 years?How do you handle disagreements?How do you manage work and family lifeHow have you grown in your ability to communicate effectively?What have been your biggest accomplishments since you started this companyHow have you customers attacked the rising input costs going into the 2023 growing season?What do you see being the biggest agronomic challenge facing growers this year?Weather, Disease, Insects, Fungicide availabilityWhat has the adoption rate been with your client base for using biologicals?Where do you see input prices going next year?What are some key things our listeners can do now to focus on maximizing the potential of this year's crop?How many of your growers use cover crops?What else did we miss?Summary and challengeKnowing what you know now, what advice would you give yourself or others at 18? Share contact information

In this episode, SPG's Agronomy Manager, Meagen Reed, sits down with Tom Wolf, a professional agrologist and co-founder of Sprayers101.com, to discuss everything from surfactants and adjuvants to optimizing your spray application. Read the New Fact Sheet on Optimizing Spray Applications Read the New Fact Sheet on Adjuvants

Pesticide applicators frequently add adjuvants to help their pesticide applications be more effective or safer to use. Adjuvants, however, are not assessed for their risk to bees by regulators. We hear about new research that assesses the safety of these products to bees.

Choosing the right adjuvant can give you an edge over hardy weeds. Successful Farming editor, Chelsea Dinterman, sat down with Bryan Young to discuss how adjuvants can complement weed management systems and potentially save you money. Learn more about your ad choices. Visit megaphone.fm/adchoices

Like last week, we are going to revisit one of the top two interviews from 2022. This conversation was with my most (personally) anticipated interview, Dr. Sandra Kaufmann. I fell in love with her first anti-aging book from several years ago and then fell in love again when she released her newest book this summer. She is a highly-respected expert in the longevity community and she didn't disappoint in this interview. Enjoy learning from Dr. Kaufmann... The author of one of my all-time favorite books recently released her second book, which was like Christmas coming early for me! Luckily, I had also met her before at an anti-aging conference and we have stayed in touch throughout the years. So I knew I had to Dr. Sandra Kaufmann on The Red Light Report for some amazing conversation about anti-aging and longevity strategies, especially as it relates to supplements and adjuvants —  topics that she is considered a bonafide expert. In her newest book, Aging Solutions, Dr. Kaufmann covers 28 different agents that she has found to be the most efficacious and backed by science, according to her rigorous research. We cover many agents from both of her books along with Dr. Kaufmann's skincare routine and other longevity hacks she as implemented over the years, including red light therapy! Dr. Sandra Kaufmann began her academic career in the field of cellular biology, earning a Master's Degree from the University of Connecticut in Tropical Ecology and Plant Physiology. Turning to medicine, she received her medical Degree at the University of Maryland, and completed a residency and fellowship at Johns Hopkins in the field of pediatric anesthesiology. She has been the Chief of Pediatric Anesthesia at the Joe DiMaggio Children's Hospital for 9 years, a nationally recognized center of excellence. Dr. Kaufmann's avid interest in the science of anti-aging begun many years ago as an intense hobby. Utilizing her knowledge in cell biology, human pharmacology and physiology, this hobby has now become a main focus. The project represents years of non-clinical research leading to the first ever, comprehensive theory of aging. She has now released two books on longevity: The Kaufmann Protocol: Why We Age and How to Stop it, and just recently in 2022, Aging Solutions.   This conversation could have easily gone on for hours and hours, as Dr. Kaufmann is a wealth of knowledge in some of my favorite topics and is fun to listen and learn from. I know you will all enjoy learning from her like I did and, if so, I highly recommend you check out BOTH of her books. They are simply amazing resources of anti-aging/longevity information. As always, light up your health! - Dr. Mike Belkowski & Dr. Sandra Kaufmann discuss the following: Her reasoning for jumping into anti-aging and longevity RAADfest - October 2022 in San Diego Supplements vs. adjuvants How people confuse deficiency with longevity Bioavailability and resveratrol  Bringing natural remedies back into the light The five basic agents of the Panacea Astaxanthin and its benefits for free radical scavenging The blood-brain barrier Nicotinamide and why it's crucially important for longevity Anti-inflammatories for longevity Glycation Eye health and vision Carnosine eyedrops Eye health and red light therapy Goggles vs. no goggles with red light therapy Supplements and adjuvants she is most excited about Spermidine and how it prevents DNA damage The mitochondrial transition permiability pore Fisetin for longevity Senescent cells (zombie cells) Metformin vs. berberine Glycation and how to defeat it How her diet doesn't affect her good lab results Her anti-aging and longevity regiment Skin health UV exposure to the skin Starting treatments earlier vs. later Micronutrient deficiencies and getting older Newfound discoveries - Watch this episode on YouTube - Where to Learn More From & About Dr. Sandra Kaufmann:   Website: KaufmannProtocol.com   Instagram: @KaufmannAntiAging   - Books written by Dr. Sandra Kaufmann:   Aging Solutions (2022)   The Kaufmann Protocol (2017)   - Check out the Kindle version of Red Light Therapy Treatment Protocols eBook, 4th Edition - To learn more about red light therapy and shop for the highest-quality red light therapy products, visit https://www.biolight.shop - Stay up-to-date on social media: Instagram YouTube

Alec sits down with Dr. Stephanie Seneff to discuss glyphosate, the childhood shot schedule, the em-are-in-ay shots, and their differences in perceptions on virology. For all of our links, visit: https://www.flowcode.com/page/thewayfwrd Stephanie Seneff is a Senior Research Scientist at the MIT Computer Science and Artificial Intelligence Laboratory.  She received the B.S. degree in Biophysics in 1968, the M.S. and E.E. degrees in Electrical Engineering in 1980, and the Ph.D degree in Electrical Engineering and Computer Science in 1985, all from MIT.  For over three decades, her research interests have always been at the intersection of biology and computation – developing a computational model for the human auditory system, understanding human language so as to develop algorithms and systems for human computer interactions, as well as applying natural language processing (NLP) techniques to gene predictions.   She has published over 170 refereed articles on these subjects, and has been invited to give keynote speeches at several international conferences.  She has also supervised numerous Master's and PhD theses at MIT.   In recent years, Dr. Seneff has focused her research interests back towards biology. She is concentrating mainly on the relationship between nutrition and health.  Since 2011, she has published over 30 papers, together with colleagues, in various peer-reviewed medical and health-related journals on topics such as modern-day diseases (e.g., Alzheimer, autism, cardiovascular diseases), analysis and search of databases of drug side effects using NLP techniques, and the impact of nutritional deficiencies and environmental toxins on human health.   Check out Dr. Seneff's book here: https://www.chelseagreen.com/product/toxic-legacy/   Some of Dr. Seneff's peer reviewed studies:   MRNA shots: 1. https://ijvtpr.com/index.php/IJVTPR/article/view/23 Worse than the disease? Reviewing some possible unintended consequences of mRNA vaccines against Covid-19.   2. https://www.sciencedirect.com/science/article/pii/S027869152200206X Innate Immune Suppression by SARS-CoV-2 mRNA Vaccinations: The role of G-quadruplexes, Exosomes, and MicroRNAs   3. https://www.hilarispublisher.com/abstract/potential-mechanisms-for-human-genome-integration-of-genetic-code-from-sarscov2-mrna-vaccination-implications-for-diseas-92500.html Potential Mechanisms for Human Genome Integration of Genetic Code from SARS-CoV-2 mRNA Vaccination: Implications for Disease   Glyphosate & Aluminum: https://www.scirp.org/html/5-3000951_53106.htm Aluminum and Glyphosate Can Synergistically Induce Pineal Gland Pathology: Connection to Gut Dysbiosis and Neurological Disease     For more on The Way Forward, please visit https://thewayfwrd.com   Do you run a freedom-oriented or holistic health oriented business? Join our FREE business directory here:  https://thewayfwrd.com/directory-form/   Join our membership here!: https://thewayfwrd.com/membership-sign-up/   Like this episode? Then you'll love The Way Forward's new membership platform! For $17/month or $150/annually, you get:   SOURCE: a freedom & health oriented business directory exclusive discounts with partner brands and products exclusive podcast episodes courses + workshops weekly community calls + Q&As with guests past and future in-person event footage past, present, and all future livestream event footage live breathwork sessions, kundalini yoga, and meditations exclusive content from Alec Zeck, Garret Kramer, Mollie Engelhart, Brandon Bozarth + more   Sign up for our membership at: https://thewayfwrd.com/membership-sign-up/   Follow The Way Forward on telegram: https://t.me/thewayforwardformankind   Like us on Facebook: https://www.facebook.com/T.Way.Forward   Follow us on Instagram: https://www.instagram.com/the.way.fwrd/   Sign up for our newsletter: https://thewayfwrd.com/newsletter/   We stream our podcasts live every Wednesday. Watch live at:   Youtube: https://www.youtube.com/@TheWayFwrd   Unite: https://unite.live/channels/the-way-forward/the-way-forward   Bitchute: https://www.bitchute.com/channel/a3s3CiyELVd8/

In dieser Episode spreche ich mit Lily über ihren Leidensweg mit dem ASIA-Syndrom (Autoimmune Syndrome Induced by Adjuvants). Bei Lily waren es die Brustimplantate, die sie so krank gemacht haben, dass sie nicht einmal mehr ihr Handy halten konnte. IG: superheldenohnecape E-Mail: superheldenohnecape@yahoo.com This podcast is hosted by ZenCast.fm

Terry Kippley, president of the Council of Producers and Distributors of Agrotechnology, says adjuvants are the most cost effective input a farmer can use to get a job done. And he explains how the industry grew from three crop protection product labels requiring the use of an adjuvant to now more than 1,000.See omnystudio.com/listener for privacy information.

The author of one of my all-time favorite books recently released her second book, which was like Christmas coming early for me! Luckily, I had also met her before at an anti-aging conference and we have stayed in touch throughout the years. So I knew I had to Dr. Sandra Kaufmann on The Red Light Report for some amazing conversation about anti-aging and longevity strategies, especially as it relates to supplements and adjuvants —  topics that she is considered a bonafide expert. In her newest book, Aging Solutions, Dr. Kaufmann covers 28 different agents that she has found to be the most efficacious and backed by science, according to her rigorous research. We cover many agents from both of her books along with Dr. Kaufmann's skincare routine and other longevity hacks she as implemented over the years, including red light therapy!Dr. Sandra Kaufmann began her academic career in the field of cellular biology, earning a Master's Degree from the University of Connecticut in Tropical Ecology and Plant Physiology. Turning to medicine, she received her medical Degree at the University of Maryland, and completed a residency and fellowship at Johns Hopkins in the field of pediatric anesthesiology. She has been the Chief of Pediatric Anesthesia at the Joe DiMaggio Children's Hospital for 9 years, a nationally recognized center of excellence.Dr. Kaufmann's avid interest in the science of anti-aging begun many years ago as an intense hobby. Utilizing her knowledge in cell biology, human pharmacology and physiology, this hobby has now become a main focus. The project represents years of non-clinical research leading to the first ever, comprehensive theory of aging. She has now released two books on longevity: The Kaufmann Protocol: Why We Age and How to Stop it, and just recently in 2022, Aging Solutions. This conversation could have easily gone on for hours and hours, as Dr. Kaufmann is a wealth of knowledge in some of my favorite topics and is fun to listen and learn from. I know you will all enjoy learning from her like I did and, if so, I highly recommend you check out BOTH of her books. They are simply amazing resources of anti-aging/longevity information. As always, light up your health! - Dr. Mike Belkowski & Dr. Sandra Kaufmann discuss the following: 3:16 - Her reasoning for jumping into anti-aging and longevity 3:29 - RAADfest - October 2022 in San Diego 8:05 - Supplements vs. adjuvants 9:27 - How people confuse deficiency with longevity 10:57 - Bioavailability and resveratrol  12:15 - Bringing natural remedies back into the light 15:00 - The five basic agents of the Panacea 15:27 - Astaxanthin and its benefits for free radical scavenging 16:33 - The blood-brain barrier 16:53 - Nicotinamide and why it's crucially important for longevity 17:42 - Anti-inflammatories for longevity 18:05 - Glycation 19:15 - Eye health and vision 19:50 - Carnosine eyedrops 21:35 - Eye health and red light therapy 22:38 - Goggles vs. no goggles with red light therapy 25:46 - Supplements and adjuvants she is most excited about 27:10 - Spermidine and how it prevents DNA damage 29:34 - The mitochondrial transition permiability pore 31:36 - Fisetin for longevity 33:22 - Senescent cells (zombie cells) 36:41 - Metformin vs. berberine 38:44 - Glycation and how to defeat it 39:05 - How her diet doesn't affect her good lab results 42:41 - Her anti-aging and longevity regiment 46:06 - Skin health 51:46 - UV exposure to the skin 54:25 - Starting treatments earlier vs. later 59:08 - Micronutrient deficiencies and getting older 1:00:09 - Newfound discoveries - Watch this episode on YouTube - Where to Learn More From & About Dr. Sandra Kaufmann:   Website: KaufmannProtocol.com   Instagram: @KaufmannAntiAging   - Books written by Dr. Sandra Kaufmann:   Aging Solutions (2022)   The Kaufmann Protocol (2017)   - Check out the Kindle version of Red Light Therapy Treatment Protocols eBook, 4th Edition - To learn more about red light therapy and shop for the highest-quality red light therapy products, visit https://www.biolight.shop - Stay up-to-date on social media: Instagram YouTube

Understanding the role of adjuvants as a part of a well-rounded crop protection program is important.

What's up to my demagogic drum fish and disputatious dunkers!   Welcome back to the BNP everyone and thank you for joining! To my patrons, you all are the spiral arms of my galaxy and the selenium in my brazil nuts- you are appreciated! Well friends, hold onto your butts, because this one's a doozy. In addition to the audio tidbits you know and love, I dive into some heady and provocative topics this week. In weeks past I've discussed a concept I refer to as "The Adversary," or the spirit of negative disruption and darkness that causes unnecessary suffering here on Earth. This week I was moved to dive into one particular aspect of this Adversary: the International Biomedical Cartel. Science only operates cleanly when there is room for dissenting opinions and troublesome hypotheses. When you are no longer allowed to question certain scientific dogmas, then it becomes a kind of religion. Hence we see the rise of Scientism and its attendant high priests' rule by fiat, selective funding and intimidation of dissent to preserve the hegemony of the establishment plunderbund.Featured in this week's episode is an interview with Kary Mullis, published in Spin magazine in July of 1994 and conducted by Celia Farber. Mullis won a Nobel Prize in Chemistry in 1993 for the invention of the PCR (Polymerase Chain Reaction) test. He became a controversial figure for refusing to stop asking pesky questions of Fauci, Gallo, and the medical establishment. Finally, I explore the potential links between the meteoric rise of peanut allergies and the use of peanut oil as an "excipient," (or adjuvant) in inoculations. Adjuvants are ingredients added to shots which compel a more intense immune response by the body. Aluminum is another commonly utilized adjuvant. Help keep the BNP on the air by becoming a patron at www.patreon.com/noetics. For as little as $1/month, you become a partner in the BNP. Help make the magic happen (and help me to afford groceries!) You also receive a dream interpretation and an original haiku when you sign up.  Let's elevate the human spirit y'all!You can also make a small one-time donation at: https://www.buymeacoffee.com/noeticsThank you so much for the support! Thank you for spreading the word and telling a friend about the BNP! Follow the BNP on IG @barbarian_noeticsEmail: barbarian.noetics@gmail.comUntil next week everyone, Be excellent to one another,and kind and loving towards yourself. One Love,Little Raven kaaaawwwwTRACKLIST FOR THIS EPISODE CSPAN Washington Journal Theme MusicRemix Maniacs - Presidential Theme Song (Trap Remix)Dykotomi - Corvid CrunkNoir Deco - Future NoirKainbeats - Atlantis (Lo Fi Mix)Soulection Radio #548 (Hi-Tek & Talib Kwali Edit)Suicide Boys x Pouya - Runnin Thru The 7th With My WoadiesKainbeats - Finding Beauty (Lo Fi Mix)idunnomane - anyways 3001Max Romeo - War Ina BabylonDillon Witherow - Before Sunrise (Lo Fi Mix)Show Dem Camp feat. Buju - Do Me Nice Edvard Grieg - Hall Of The Mountain King (Deficio Remix)The Village People - Macho Man, In The NavyLINKSInterview with Kary Mullis (Spin Magazine, July 1994)https://thedoctorwithin.com/vaccines-and-the-peanut-allergy-epidemic/ Support the show

Mandy and Sarah are joined by Greg Dahl to discuss adjuvants and how you can utilize them. 

Welcome to The Nonlinear Library, where we use Text-to-Speech software to convert the best writing from the Rationalist and EA communities into audio. This is: Postmortem on RatVac, published by caffemacchiavelli on January 22, 2022 on LessWrong. Note: The name is a tongue-in-cheek abbreviation for "Rationalist Vaccine", we have no association with the RaDVaC project and use a much simpler, almost trivial approach. I don't endorse making your own vaccine or taking anything nerdy people on the Internet send you. This is not medical advice. tldr: In April of 2021 I assembled my own subunit vaccine against the Beta variant of SARS-Cov-2 ("SARS2"). Despite starting with the prior that this formulation should be somewhat effective, I could not demonstrate efficacy against the Alpha variant in antibody tests. Introduction I started getting interested in DIY vaccines soon after the Making Vaccine and We got what's needed for COVID-19 vaccination completely wrong posts were published. Particularly the idea of just making a standard subunit vaccine appealed to me, so I teamed up with some interested people from the rationalist diaspora and we ended up making our own vaccine candidate in early to mid 2021. Some contributed advice, many contributed funding (for which I am still extremely grateful), and while the project wasn't the clear success I had hoped for, maybe the true treasures are the friends we gained and the lab equipment we bought along the way. Recombinant Vaccine ELI5 Subunit vaccines contain a subset of a single protein molecule. For viruses this will typically be a protein that contains the receptor-binding-domain (RBD), i.e. the part that actually binds with the host during infection. Through the wonders of genetic engineering - see very short introduction below - we can produce these proteins and then simply introduce them into the human body. While Yang et al have shown that an immune response occurs even with a vaccine purely containing viral RBD, the response can be enhanced by adding an immunologic adjuvant. Adjuvants are a very diverse class of chemicals, ranging from simple mineral salts to proteins or even small cells. The only thing they ostensibly have in common is that they're used to increase or modulate immune response, and that's why we're using them. Genetic Engineering ELI5 For those curious about how we can manufacture proteins to begin with - because I definitely was - here is a very short and abridged intro. The process differs depending on the method used, but usually roughly follows these steps: Encode and synthesize the amino acid sequence of the protein as DNA. Add this DNA to an expression vector. These are typically either viruses or circular strings of DNA called plasmids. Introduce your expression vector into an expression host, often bacterial, yeast or mammalian cells. This is called transfection and can take several forms, from chemical and viral transfection to just shooting cells with a "gene gun" that fires microscopic gold particles coated in DNA. Select for the transfected cells. This is usually done by adding a gene for antibiotic resistance to the expression vector, so that you can then kill all non-transfected cells by said antibiotic. Grow and maintain your culture until sufficient amounts of protein have been produced, then extract and purify the protein. The details will vary depending on protocol and I'm deliberately sticking to the basics and leaving out things like CRISPR, RNA plasmids and plant hosts. More important is imo that while this may sound extraordinarily complicated, it's very much doable by a single individual or small team in a self-funded lab. DNA can be synthesized for hundreds of dollars and often already shipped as a customized plasmid. Host cells and growth media are also affordable (at least at the mg-µg scale we're aiming for, don't try making your own burger meat), which leaves purification. I'm not too happy with any of the DIY methods I've seen so far,...

Link to original articleWelcome to The Nonlinear Library, where we use Text-to-Speech software to convert the best writing from the Rationalist and EA communities into audio. This is: Postmortem on RatVac, published by caffemacchiavelli on January 22, 2022 on LessWrong. Note: The name is a tongue-in-cheek abbreviation for "Rationalist Vaccine", we have no association with the RaDVaC project and use a much simpler, almost trivial approach. I don't endorse making your own vaccine or taking anything nerdy people on the Internet send you. This is not medical advice. tldr: In April of 2021 I assembled my own subunit vaccine against the Beta variant of SARS-Cov-2 ("SARS2"). Despite starting with the prior that this formulation should be somewhat effective, I could not demonstrate efficacy against the Alpha variant in antibody tests. Introduction I started getting interested in DIY vaccines soon after the Making Vaccine and We got what's needed for COVID-19 vaccination completely wrong posts were published. Particularly the idea of just making a standard subunit vaccine appealed to me, so I teamed up with some interested people from the rationalist diaspora and we ended up making our own vaccine candidate in early to mid 2021. Some contributed advice, many contributed funding (for which I am still extremely grateful), and while the project wasn't the clear success I had hoped for, maybe the true treasures are the friends we gained and the lab equipment we bought along the way. Recombinant Vaccine ELI5 Subunit vaccines contain a subset of a single protein molecule. For viruses this will typically be a protein that contains the receptor-binding-domain (RBD), i.e. the part that actually binds with the host during infection. Through the wonders of genetic engineering - see very short introduction below - we can produce these proteins and then simply introduce them into the human body. While Yang et al have shown that an immune response occurs even with a vaccine purely containing viral RBD, the response can be enhanced by adding an immunologic adjuvant. Adjuvants are a very diverse class of chemicals, ranging from simple mineral salts to proteins or even small cells. The only thing they ostensibly have in common is that they're used to increase or modulate immune response, and that's why we're using them. Genetic Engineering ELI5 For those curious about how we can manufacture proteins to begin with - because I definitely was - here is a very short and abridged intro. The process differs depending on the method used, but usually roughly follows these steps: Encode and synthesize the amino acid sequence of the protein as DNA. Add this DNA to an expression vector. These are typically either viruses or circular strings of DNA called plasmids. Introduce your expression vector into an expression host, often bacterial, yeast or mammalian cells. This is called transfection and can take several forms, from chemical and viral transfection to just shooting cells with a "gene gun" that fires microscopic gold particles coated in DNA. Select for the transfected cells. This is usually done by adding a gene for antibiotic resistance to the expression vector, so that you can then kill all non-transfected cells by said antibiotic. Grow and maintain your culture until sufficient amounts of protein have been produced, then extract and purify the protein. The details will vary depending on protocol and I'm deliberately sticking to the basics and leaving out things like CRISPR, RNA plasmids and plant hosts. More important is imo that while this may sound extraordinarily complicated, it's very much doable by a single individual or small team in a self-funded lab. DNA can be synthesized for hundreds of dollars and often already shipped as a customized plasmid. Host cells and growth media are also affordable (at least at the mg-µg scale we're aiming for, don't try making your own burger meat), which leaves purification. I'm not too happy with any of the DIY methods I've seen so far,...

Professor Nikolai Petrovsky is the Director of Endocrinology (Flinders Medical Centre), Professor of Medicine (Flinders University), Vice President of the International Immunomics Society, Founder of Vaxine and the creator of COVAX-19 and Spikogen. He joins us for the second time in the follow up interview to discuss the progress of his traditional protein vaccine (COVAX-19/Spikogen), the medical ethics of mandating vaccines, a comparison of vaccine types, concerns with mRNA and adenoviral vector vaccines, vaccine adverse event incidence rates, and the scientific errors being made by politicians conflating community protection with individual protection. As a vaccine creator, Professor Petrovsky is very vocal on his LinkedIn account against the mandating of vaccines, especially with no scientific justification. Some may miss parts in this interview where he nonchalantly drops bombshells regarding his now approved protein vaccines. It may be the case that we have successfully created a traditional vaccine, right here in Australia, that completely outperforms the major products on the market today AND has a significantly lower adverse event rate. As data is released showing the performance of his and other competitor protein vaccines (such as NovaVax) it will raise sticky questions for Australian Health Minister Greg Hunt MP: 1. Why has the Australian government refused to engage with Australian vaccine creators? 2. Why has CSL refused to cooperate with Australian vaccine companies? 3. What are in those secret commercial contracts with Pfizer et al? 4. Are there clauses that prevent competition and consideration of products that are actually safer for the Australian population? Watch this episode on Youtube: https://youtu.be/9x2ieHuj8zU ---------------------------------- PROFESSOR NIKOLAI PETROVSKY www.linkedin.com/in/petrovsky https://vaxine.net DISCERNABLE Our Private Community: discernable.locals.com The Crew Mailing List: https://discernable.io/crew The Video Archive: https://www.youtube.com/discernable The Podcast: http://discernable.io/listen ---------------------------------- 2:04 Should people be concerned about current Covid-19 vaccines? 7:07 Why mandates are not scientifically justified. 10:37 The transmissibility of Covid-19/Sars-Cov2 13:02 Current vaccines won't get us ‘back to normal' 15:50 Current vaccines do not provide community benefits, only individual benefits 18:59 CSL refuses to cooperate with Australian vaccine creators 23:07 Big pharma has zero liability 26:49 Liability waivers are given selectively 30:13 Government contracts are shrouded in secrecy 33:16 The mass silencing of concerned individuals in Australia 36:32 The silence from medical professional associations 38:54 The government is downplaying vaccine adverse events 44:42 What are normal rates of adverse events from traditional vaccines? 48:48 Should children be vaccinated against Covid-19? 55:53 What is the COVAX-19 vaccine? 59:30 What happened in the clinical trials? 1:02:24 COVAX-19 phase 3 trial data compared to the other vaccines 1:04:03 COVAX-19 approved and rolling out now 1:05:26 Will COVAX-19 be approved in western countries? 1:07:27 The science behind COVAX-19 1:08:15 MRNA vaccines vs protein-based vaccines 1:13:05 Adjuvants used in COVAX-19 1:14:16 Will COVAX-19 need boosters? 1:16:22 BREAKING: Sterilising immunity of COVAX-19 1:20:19 What about natural immunity? 1:25:27 Making your voice heard to defend democracy 1:28:27 The pandemic has exposed failing systems worldwide

Compaction problems are even worse in many areas this year due to dry conditions.

Please join Dr Maggi and Dr Harandi as they discuss how vaccine adjuvants can enhance immunity in older adults. Credit available for this activity expires: 8/13/2022 Earn Credit / Learning Objectives & Disclosures: https://www.medscape.org/viewarticle/956558?src=mkm_podcast_addon_956558

ICL's Sam Rivers and Syngenta's Glenn Kirby talk about using adjuvants to mark ICL and Syngenta's launch of Elasto G5. 

James Reiss, Vice President of Product Development with Precision Labs joins Michele for a podcast on adjuvants. James will talk about where adjuvants got their start, how we got to where we are today, and what the future looks like for adjuvants.  Jim talks about Precision Lab's partnerships with CPDA and much more in this podcast that is sponsored by Precision Labs. 

Join Matt Faletti, Products Portfolio and Marketing Manager for Precision Laboratories, LLC, to learn about more about adjuvants.  Matt and Michele discuss APE and NPE adjuvants and their history, adjuvants with fungicide application and Precision Labs newest adjuvant, Precisive.This podcast is sponsored by Precision Laboratories, LLC.  For more information on adjuvants please contact your AFS agronomist.  

Vaccines help our immune system to learn to recognize invading pathogens before we ever get infected. But some vaccines include molecules that act like an extra alarm system, alerting our immune cells to pay attention! These molecules are called adjuvants, and scientists are working on creating new ones that could help create vaccines against pandemics that haven’t even happened yet!

Farmers want to make sure they are efficient when applying fertilizers, but some factors are out of their control.

BPS agriculture’s Augere is a fertilizer enhancing adjuvant for improving the uptake of nutrients and reducing leaching.

Welcome to another episode of the Dr. Lo Radio Show! I really enjoyed recording this episode, and I think you will enjoy it as well. Today I am chatting with Sandra Kaufmann all about anti-aging.  We specifically talk about the seven tenets of aging. Sandra is the founder of The Kaufmann Anti-aging Institute, which is an educational company that focuses on why we age and how we can minimize the effects of aging to live longer and healthier.  In this episode, Sandra shares so much good information including how to understand cellular aging, why different people age differently, and why longevity protocols need to be personalized.  She also shares the best way to get started on a protocol and why a longevity protocol is important in addition to diet and exercise.   If you are listening to this episode and you or someone you know are navigating through any of the health issues we discuss, I would be more than happy to support you in the journey! Feel free to reach out to me to continue this conversation: @doctor_lo I hope you enjoy and learn something new from this episode!  We Discuss: {09:36} Sandra shares her story and background {13:13} The 7 Reasons Your Cells Age/The Seven Tenets of Aging {16:20} What is Autophagy? {21:24} Your Immune System's Effect on Aging {24:01} Lipofuscin {25:52} How Genetics Affect Your Aging {28:29} Assessing Root Causes {30:24} Using Molecular Agents (Supplements, Adjuvants, & More) {33:10} Agents That Help the Most Overall {43:05} Quercetin Benefits {44:40} Ecklonia Cava Benefits  {46:37} Diet and Exercise Effects on Anti-Aging {48:37} Sandra’s Personal Protocol  {51:25} Tips for Getting Started Using Molecular Agents {54:36} What Most People Don’t Know About Collagen And so much more!  Links Mentioned: Sandra’s Website https://kaufmannprotocol.com/the-company The Kaufmann Protocol: Why we Age and How to Stop it https://www.amazon.com/Kaufmann-Protocol-Why-Age-Stop/dp/0692089047 Sandra on Instagram https://www.instagram.com/kaufmannantiaging/ Wine, Women, & Hormones Event https://www.lavitarx.com/Events Hiya Health http://www.hiyahealth.com/drlo $50 off first order CODE: DRLO Paleovalley https://paleovalley.com/ 15% off with CODE: DRLO About The Guest:  Sandra Kaufmann is the founder of The Kaufmann Anti-aging Institute, a forward-thinking educational company, with the overarching goal of educating the general public on why we age and how we can minimize the effects of aging to live longer healthier lives. The Kauffman protocol, which has both a book and app on the market, is the product of this and is underpinned by Sandra's longevity and cellular biology expertise. Dr. Kaufmann has a Master's Degree from the University of Connecticut in Tropical Ecology and Plant Physiology. She is a member of the Scientific Advisory Board and has recently received an accolade from the American Health Council as "Best in Medicine," she is a certified expert on the science of medicine, and specifically anti-aging. Her avid interest in the science of anti-aging began many years ago as an intense hobby. Utilizing her knowledge in cell biology, human pharmacology, and physiology, this hobby has now become a main focus. The project represents years of non-clinical research leading to the first, ever, comprehensive theory of aging. Follow me on social media for fertility tips, daily life, and more!  - Instagram: @doctor_lo: https://www.instagram.com/doctor_lo/ - Facebook: Dr. Lauren Noel: https://www.facebook.com/DrLoNoel/ - Shine Instagram: Shine Natural Medicine: http://www.instagram.com/shinenaturalmedicine - My Golden Eggs Fertility Course: https://www.goldeneggsfertility.com If you enjoyed this episode, please leave a 5 star review on Apple Podcasts, Google Play, or wherever you are listening! And don't forget to subscribe and share this podcast with friends and family! I love seeing your posts on social media! 

What's an adjuvant, you ask? They're inert ingredients used in crop protection products to make the chemistry more efficient and effective. Joe Gednalske with the Council of Producer & Distributors of Agrotechnology joins me to discuss technology in crop production. Joe holds 38 patents and has been in the Ag industry for half a century. Which is why I encourage you to share this episode with your non-Ag friends — because, as Joe explains — many adjuvants used in pesticides are also found in detergent, shampoo, and home cleaning supplies. Sponsored by Harvest Profit harvestprofit.com and LandTrust landtrust.com

Psychobiotics: the complex interactions between the gut microbiome and the brain and how to support a healthy brain; Ignoring dental health is a problem leading to inflammation and dementia; Current vaccines do help against the variants of Coronavirus; Supplements to aid microbiome health and address its effect on rheumatoid arthritis; The delicate balance of using Adjuvants to augment the immune response of Coronavirus vaccines; A complex autoimmune disease discussion including Epstein Barr virus; Do some home food allergy testing kits exaggerate results? The problem of homogeneous science study groups that are not generalizable to real populations

Psychobiotics: the complex interactions between the gut microbiome and the brain and how to support a healthy brain; Ignoring dental health is a problem leading to inflammation and dementia; Current vaccines do help against the variants of Coronavirus; Supplements to aid microbiome health and address its effect on rheumatoid arthritis; The delicate balance of using Adjuvants to augment the immune response of Coronavirus vaccines; A complex autoimmune disease discussion including Epstein Barr virus; Do some home food allergy testing kits exaggerate results? The problem of homogeneous science study groups that are not generalizable to real populations

Adjuvants: what are they and why is Cillian obsessed with them? They are particularly important in cancer vaccines and vaccines against some of the more complex pathogens, but that's just the beginning! 

Andrew Read, Ph.D is the director of Huck Institutes of the Life Sciences, the Evan Pugh Professor of Biology and Entomology, and the Eberly Professor of Biotechnology at Pennsylvania State University. He joins host Lauren Richardson to discuss the results and implications of the article "An adjunctive therapy administered with an antibiotic prevents enrichment of antibiotic-resistant clones of a colonizing opportunistic pathogen"  by Valerie J Morley, Clare L Kinnear , Derek G Sim, Samantha N Olson , Lindsey M Jackson, Elsa Hansen, Grace A Usher, Scott A Showalter, Manjunath P Pai, Robert J Woods, and Andrew F Read, published in eLife.

Adjuvants can make or break a spray mix, so how do you may sure that you're adding the right one to the sprays that's you're doing on your farm? Listen to today's Tec talk for a basic overview of what adjuvants are and what they can do in your mix. For more check out the Adjuvant book by GRDC linked to here https://grdc.com.au/__data/assets/pdf_file/0030/384519/10562-GRDC-Adjuvants-guide-LQ.pdf?utm_source=website&utm_medium=download_button&utm_campaign=pdf_download&utm_term=National&utm_content=Adjuvants%20Booklet

Jason and Kyle finish their conversation on adjuvants and wrap up with drift/deposition agents and water conditioners. Chapter Notes: 6:00 - Start of Topic8:00 - Drift and Deposition Agents27:30 - Water Conditioning Agents59:00 - Brew Talk

Jason and Kyle talk through adjuvants and their use with crop protection products. Part 1 gives an overview of adjuvants sold in the agriculture market and dives into the classes of utility adjuvants, Surfactants, Oils, and topic starter on Drift/Deposition agents. Cliff Notes: 5:35 - Start of Topic10:20 - Utility Adjuvants22:20 - Surfactants29:08 - Oils40:30 - Drift/Deposition Agents45:25 - Brew Talk

"Curb Your Enthusiasm: Local Anesthetic Adjuvants for  Peripheral Nerve Blocks," by Yatish S. Ranganath, MD, Clinical Assistant Professor, Program Director for RAAPM Fellowship; Melinda Seering, MD, Clinical Associate Professor, Clinical Director for Ambulatory Surgical Center; and Anil A. Marian, MD, FRCA, Clinical Associate Professor, Vice Chair for Clinical Operations; all of the Department of Anesthesia, University of Iowa Carver College of Medicine, Iowa City, Iowa. From ASRA News, November 2020. See original article at www.asra.com/asra-news for figures and references. This material is copyrighted.     

After UK Transition: Working with Chemicals - Episode 1 - PPP Plant Protection Products (PPP) - Rachel Brown - PPP lead in CRD (Future Readiness) Transition Programme at HSE and Duncan Williams - Lead for Pesticides Post 2020 Transition Project at DEFRA. We discuss what the UKIM bill means for PPP products/applications, the implications for businesses involved in the manufacture, distribution and supply of pesticides and the data businesses will need to supply to HSE.   Visit our website for further information and detailed guidance on the chemical regimes.    *The information in this podcast was correct at time of publication. Podcast transcript Good day and a warm welcome to the HSE podcast brought to you by the Health & Safety Executive to guide you through the changes in chemical regulations which are happening as a result of the UK leaving the EU.  In this episode we'll be hearing from our experts about how plant protection products, otherwise known as PPP Regulations, will be changing from the 1st January 2021 once the transition period is over.  From that date a new independent pesticides regulatory regime will operate and Great Britain will take responsibility for placing PPP's on the market in this country. My name is Mick Ord and before I introduce you to our guests, I'd like to point you in the direction of the HSE website where the information we refer to today can be found.  The place to go is www.hse.gov.uk/brexit.  And what I'd also urge you to do is to subscribe to our free bulletin service to stay up to date with all the latest news and guidance on pesticides so you have all the relevant information at your fingertips and you can do this via the website. Rachel Brown is the HSE's Transition Programme Manager for Pesticides and Duncan Williams is Team Leader for Pesticides at Defra, the Department for Environment, Food and Rural Affairs. Mick: Duncan I'll start with you if I may.  What are the main changes that will apply to plant protection products from January 2021 in terms of PPP regulations? Duncan: Ok, so at the end of this year the transition period for leaving the EU will end so that means from the 1st January next year we'll be operating a new independent pesticides regulatory regime in Great Britain by which I mean England, Scotland and Wales.  It's slightly different in Northern Ireland, so we'll come onto them shortly.  So in Great Britain the EU Legislation is essentially carried over into national law.  So, all the regulatory requirements of the current EU regime, all the technical standards, are carried over unchanged into our national regime.  It will look and feel very similar to the current EU regulations, except we'll be taking our own decisions rather than being covered by EU decisions. That means there'll be some degree of divergence from EU decisions over time for example maybe different decisions at different times or sometimes different decisions and of course any EU decisions which come into force after the end of this year, won't apply in Great Britain.  If they come into force before the end of this year, they will and they will be carried forward.  So that's things like active substance decisions, Maximum Residue Level (MRL) setting and so on. Mick: What about businesses operating in Northern Ireland – what's going to happen to them? Duncan: Ok, so under the Northern Ireland Protocol, the EU Plant Protection Product Regulations and also the EU Maximum Residue Level Regulations – they'll continue to apply directly in Northern Ireland so there's less change.  So in Northern Ireland the position is basically remaining as much as it is now during the transition period.  That means EU active substance approval decisions, MRL decisions, they will all continue to apply directly in Northern Ireland.  They'll still have responsibility for product authorisations under that EU Framework and that function will continue to be delivered by HSE for Northern Ireland. Mick: How similar will the new GB regulations be to those from the old EU process? Duncan: Very similar.  The name of the legislation actually remains the same.  We'll still be referring to EU Regulation 1107/2009 as it applies in Great Britain.  Some changes have been made to the legislation so that the regulations can operate properly in a national context outside the EU.  For example the EU Regulations for various centralised decision making processes and so on built into them which have had to be replaced by national processes but there's no substantive policy change. All the retained EU law, retained EU law is what we call the legislation we'll keep in Great Britain afterwards, that's been put online at legislation.gov.uk  and you can see the changes which have been made to it.  The regulatory requirements remain the same and the body of EU technical guidance which underpins the legislation that's used to make decisions, all of that is carried over as well and will continue to be used in Great Britain after the 1st of January so there's no change in the standards of protection, the big difference is that decisions will be taken by national bodies rather than through EU processes and EU institutions.  So that's HSE, Defra and the Scottish and Welsh Governments working closely together. HSE remains the national regulator for the whole of the United Kingdom on behalf of the UK Government and the devolved administrations in Scotland, Wales and also Northern Ireland.   HSE will continue to do the majority of the work and decision making and we'll all be working very closely together in a shared governance framework.  HSE will continue to accept applications in much the same way they do now. Mick: Rachel, how will the application forms be different to those from the old EU process? Rachel: So as Duncan says, the name of the legislation actually remains the same, so we are still going to be referring to EU Regulation 1107/2009, as it applies in Great Britain and this means that a lot of our templates and application forms will actually appear to be unchanged.  We will continue to accept applications in the same way as we do now, and will still need a registration report in the same format in support of an application and there'll only be some really minor changes to the application form such as whether an application is relevant for GB or NI and these will be published on our website in time for use after Day 1. Mick: Will existing PPP Product authorisation previously granted by the UK still be valid and if so for how long? Rachel: Yes, on Day 1, all the existing Plant Protection Product authorisations previously authorised in the UK, they'll continue to be valid in both GB and NI. We won't be issuing any new product notices at all.  The existing notices and their current expiry dates will all remain unchanged. Mick: Will GB continue to issue parallel trade permits from 2021? Rachel: We will no longer be able to accept applications for parallel permits into Great Britain.  These are an EU measure and we just can't operate that outside the EU context. However, we have put in place some transitional measures to allow time for businesses to adjust and can continue to issue parallel trade permits for sale and use in Northern Ireland. Mick: How long will current parallel trade permits be valid for? Rachel: So for GB, the existing permits which were granted prior to 31st December 2020, they'll be allowed to continue until 31st December 2022 or they may expire on their existing expiry date if that is an earlier date before December 2022.   The withdrawal action will be published for all those existing parallel import permits and we are hoping that will be done in the first part of 2021.  This will allow time for stocks to be used up or for any new applications made under national regimes if necessary. Mick: Back to you again Duncan.  What is the situation with reference to active substance approval – will they continue to be valid until their expiry date? Duncan: On Day 1, 1st January 2021, all active substance approvals which are in place will continue to be valid, they'll be carried forward in Great Britain obviously the EU regime continues to apply in Northern Ireland anyway so no changes there. We'll have a new statutory register for active substances in Great Britain. This will be clearly published on the HSE's website and it will show all the active substances which are approved in GB.  It will replace the EU's list of active substances which are in Commission Implementing Regulations if you are familiar with those and the content is the same at Day 1.   So all of those active substance approvals   are carried forward and with that new national statutory register, basically an active substance has got to be approved and listed on that register for it to be included in any Plant Protected Products for use in GB. It's worth noting that we've made some transitional provisions so that the old EU regime can link smoothly to the new national one.  One of these is that in Great Britain, the expiry dates of active substances where they were due to expire in the EU within 3 years of the end of the transition period. Those ones will be granted a 3 year extension under the Great Britain regime and this is because applications for renewals have to be made 3 years before the expiry date and obviously if it's within 3 years of the end of transition, that date will have already passed when the national regime comes into force so the extension is necessary to have an orderly transition to the new regime.  So this means active substances which were due to expire in the 3 year period from 1st January 2021 right through to the end of 2023, they will be extended for a further 3 years in Great Britain.   Just for reassurance, we can act sooner if we needed to for any reason so for example if there was new important data or scientific evidence about concerns with a particular substance, then there is provision within the legislation for action to be taken sooner should that be warranted. Mick: What happens when it expires Duncan? Duncan: If an active substance approval expires then obviously the products relating to that active substance have to be withdrawn but if you want a renewal for that active substance, then you need to make an application for that renewal under the GB regime.  That application will be assessed by the HSE and then a decision will be taken under the GB regime.  Obviously, that would just apply in Great Britain not Northern Ireland or in the EU.  We will be developing a system for renewing approval of active substances – I mentioned we would have a 3 year extension to the expiry dates for those active substances which are due to expire within 3 years and that will allow time for us to finalise and apply the new ways of working and any new EU decisions on active substances or on Maximum Residue Levels which are taken and come into force before the end of this year, those ones will be carried over.  If those EU decisions are after the end of this year, then they are not carried over and the applications are needed under the GB regime. Mick: Will companies need to supply different data to the HSE than they were required to do when we were in the EU? Duncan: So the documentation supporting an application will, as far as possible, be the same as it is now so HSE will accept applications in the same way.  They will still require registration reports or assessment reports in the same format in support of applications for authorisations or approval. Mick: And so will GB continue to accept alternative sources of active substances deemed equivalent by the EU? Duncan: Again, it will be a GB process for that.  You'd need to submit an application to HSE and it's basically the same process as is currently in place to establish the equivalence of an alternative active substance source that's followed now. Mick: Rachel, the UK will no longer have a formal arrangement for mutual recognition.  What are the implications for businesses involved in the manufacture or distribution and supply of pesticides? Rachel: Yes, that's right Mick.  For Great Britain, HSE can no longer accept any applications for mutual recognition of authorisations in EU member states.  Any existing ones will continue in place though, if we've already granted the authorisation before the end of December.  The exception is that for applications considered under the Northern Ireland regime, we will be able to accept mutual recognition applications.  If we've got any applications on going, ones in the queue that haven't been completed yet, these will be completed to conclusion under the GB national regime and going forward for authorisation in GB you will be required to submit a stand alone GB application. Mick: In 2024 businesses involved in the production and supply of treated seeds in GB will need to do things differently as well – can you just explain the changes? Rachel: Yes, this is quite a specific area but under the EU regulations, seed can be treated in any member state with a product authorised for that purpose in that member state.  That treated seed can then be traded and used freely in any other member state.  This is going to change in the future for Great Britain.  So again, we've put some transitional provisions in place to allow time for businesses to adjust.  The current arrangements will be allowed to continue in Great Britain until 31st December 2023. So until that date, any seed which has been treated with a product authorised for that purpose in another member state can still be traded and used in Great Britain.  However, after the 31st December 2023, only seed which is treated with a product authorised for that purpose in GB can be traded and used.  Now this could mean that new authorisations for use in GB may be required so that's why the current arrangements are being allowed to continue until 2023 to allow time for the necessary applications and authorisations to be made to the GB authority.  Any existing arrangements in Northern Ireland, can continue to apply. Mick: And how will the adjuvant application process work after 1st January 2021? Rachel: So for adjuvants there isn't actually a change there.  The process for registering adjuvants in the UK will remain as it currently is.  Adjuvants are currently considered under national rule, rather than EU rule so there'll be no change for GB and NI.  All the existing adjuvant registrations will remain valid in GB and NI going forward. Mick: Duncan, how will Maximum Residue Levels (MRLs) be set in GB after 1st January 2021? Duncan: Existing MRL's at the end of the year are all carried over into the national regime so there's no immediate, practical change.  After 1st January, we will be making our own decisions in GB, setting MRLs based on our own assessments so you'll need to submit an application to HSE to set an MRL in GB.  The regulatory framework, the technical guidance for setting those MRLs, is all carried over and we will have a statutory register for GB MRLs which will be publically available on the HSE website.  It's worth noting this means that whilst MRLs in GB and the EU and Northern Ireland will be aligned as of 1st of January, moving forward there will be some degree of divergence from the EU MRLs over time. Mick: And what does the UK Internal Market Bill mean for PPP products and applications? Duncan: The UK Internal Market Bill is currently going through Parliament and the Bill includes a new principle of mutual recognition to allow the operation of the UK internal market for goods.  That will allow any goods which meet the relevant regulatory requirements relating to sale, in the part of the UK where it's produced or imported into, to then be sold in any other part of the UK.  Now this new mutual recognition principle will not affect product authorisation or active substances approvals.  Each part of the UK will remain responsible for deciding which pesticide products can be authorised for sale and use in their respective territories just as they are now, though in practice we almost always have joint decisions applying across the piste by agreement. The position is slightly different for Maximum Residue Levels.  The intention of the Bill is that the mutual recognition principle will apply to rules on Maximum Residue Levels in the same way as it will apply to rules on goods generally to ensure effective functioning of the UK's internal market.   This will avoid the potential for new internal trade barriers on sale of food which has been produced lawfully should there be any divergence in Maximum Residue Levels decisions.  This ensures that treated produce from Northern Ireland is able to be placed on the market in Great Britain for example.   There's a caveat in that EU rules will continue to apply directly in Northern Ireland itself so treated produce will only be able to be placed on the market in Northern Ireland in accordance with the EU MRL regulations.  In practice, all the administrations delegate their regulatory functions to HSE to undertake on their behalf so that helps to ensure a consistent approach.  We've got structures in place to work closely together to continue to take joint decisions wherever possible. Mick: I'm going to now ask you both specific questions for specific sectors.  What actions do people and businesses need to take - Rachel? Rachel: So, if you are a pesticide manufacturer or authorisation holder, the key message is that current authorisations and approvals do all remain in place, but for the future you need to consider where you wish to make your new pesticide applications.  So, from the 1st January 2021, you are going to have to make applications under both the GB and EU regimes to gain access to both GB and Northern Ireland markets. Future applications for renewal of current authorisations and approvals will also need to be made under both the Great Britain and EU regimes.  Do talk to HSE about your expected pipeline of applications.  Do come and ask us any questions if you are unsure of what to do. Duncan: If your business is involved in trading in food produce, the main thing to be aware of is that from 1st January next year, the Maximum Residue Levels in Great Britain and the EU might start to diverge over time.  I think the key takeaway is just to ensure you understand the requirements in your target market.  It's not for immediate action because they'll all be the same at Day 1, but they'll likely diverge over time.   If you are a pesticide user or someone who advises on pesticides, again there's no immediate change, the same products will be authorised on the other side of the end of the year, so in the short term just continue to use only authorised products always following the instructions on the label.  Over time there may be some extra things to think about if you're growing food produce intended for export for example to the EU and again, make sure you understand  the requirements for treated produce in your target market as it might affect the choices  you make about what pesticides to use. Mick: So, quite a lot to take in if you're involved in the manufacture, supply or distribution of pesticides.  Remember you can rewind the podcast at any time or listen again at your convenience.  A big thank you to Rachel and Duncan our resident experts on PPP.  You can, of course, also logon to our website, www.hse.gov.uk/brexit, where you'll find in written form, everything we've been chatting about today and much more.   And as I mentioned earlier, you can also subscribe to our free bulletin service to keep you abreast of the latest news and updates as it affects your sector.  You can do this via the website.  You'll find a subscription box on every page and you can just pop in your email address and leave the rest to us to keep you informed. Well, that's all from me, Mick Ord until the next episode, so until then, take care.

Growing up in the family floriculture business, Drew Guffey, learned a thing or two about stretching his father's dime.  Input costs are what ultimately drive the bottom line in most horticulture businesses.  The same applies to the cultivation of Cannabis (low THC hemp or medicinal/recreational).  Crop health and protection products are typically a large line item in the cultivation budget.  In this episode of the #LeadersTalkCrops series, Drew, the Alternative Segment Lead at Winfield United, shares how the use of adjuvants and wetting agents allow you to be more efficient and cost-effective when using certain crop protection and nutrition products. 

Listen to this article from Progressive Crop Consultant by Franz Neiderholzer and Rhonda Smith

The RSB Show 3-11-20 - Coronavirus update, Silver, Rabies, Cancer, Ty Bollinger, Vit C, Adjuvants by Robert Scott Bell

There are great challenges to insure safety in a product that is called "unavoidably unsafe" by the U.S. Supreme court. This episode we will review what the experts at the World Health organization vaccine safety summit in Switzerland dec. 2019 Don't miss this life changing episode when the experts are challenges with hard questions from real doctors in the field.  

Recording from the 2019 BCG Main Field Day Nitrogen management and novel methods of application - James Hunt and Nilou Nasrollahi (La Trobe University). Solving the muck and mystery of adjuvants (session 2) - Andrew Somervaille (Jubilee Consulting).

Recording from the 2019 BCG Main Field Day. Solving the muck and mystery of adjuvants - Andrew Somervaille (Jubilee Consulting).

When treating acute and chronic pain conditions, there is a need for “balanced” analgesia or multimodal analgesia. These are cases in which opioids as monotherapy are rarely appropriate. The therapeutic role of adjuvant analgesics is to increase the therapeutic index of opioids by producing an opioid-sparing effect. The use of nonopioid analgesics, adjuvant agents, and, in some cases antidepressants, may provide additional pain relief by opioid-sparing effects. Many of these agents have additional benefits in treating other related comorbid conditions present in those who suffer from chronic pain. Newer regulatory guidelines, like the CDC Guideline for Prescribing Opioids for Chronic Pain, recommend that first-line treatment for acute and chronic pain should be nonopioid analgesics as an initial trial, emphasizing the need to optimize multimodal analgesia including nonpharmacologic interventions to improve outcomes. It is vital to have an appreciation and knowledge of alternative pain treatments in an era where mass opioid use has been the norm. During the so-called opioid epidemic, practitioners should provide patients with effective tools to help manage pain while minimizing the negative effects of opioid exposure. Adjuvant agents are not primarily identified as analgesic in nature but have been found in clinical practice to have either an independent analgesic effect or additive analgesic properties when used with opioids. Adjuvants add a unique action in opioid-resistant pain and can play a role in reducing opioid side effects. Knowledge of this class of medications is critical for the prescriber to be able to document their thought process in treatment plan development in the event of regulatory review. The goal of this course is to provide you with the tools to successfully evaluate the appropriate role of these agents in your practice. (Recorded at PAINWeek 2018)

Dr Betty Martini, MPWHI.org, MPWHI.com, Toxic Sweetners Immune Adjuvants, Cotoxic Sweetners with Electropollution, Cell Phones Smart Meters Cell Towers 5G Cotoxins, End of Normal Biology, In Chewable Medicines, Mislabeled in Food, Sweetners of Death Attach to Proteins to Sensitize Autoimmunity, MS Neuropathy Heart Failure Lupus Arthritis etc., Autism Linked to Vaccines, Book Autism Vaccine Author Maready coming On Show, Dr Betty Federal Death Agency, Fatal Drugs ALlowed, Dr Bill Deagle MD AAEM ACAM A4M, NutriMedical Report Show, www.NutriMedical.com, www.ClayandIRON.com, www.Deagle-Network.com,NutriMedical Report Show, MY COMMENT: Every adult, parent, nurse, and physician should watch the following video. It is not about banning vaccines, but protecting the freedoms of those who object to all vaccines or certain vaccines. Making our own FREE choices as to how we care for our bodies and that of our children under the protection of the American Constitution which is being violated based on corrupt, fake science in the CDC, FDA, Mainstream Media, and the Pharmaceutical Industry and backed by politicians corrupted by Big Pharma Lobbyist. Many states are passing laws for forced vaccines on not only children, but adults as well without “informed-consent,” abolishing health issues and religious exemptions. For those who refuse blood transfusions and refuse vaccines due to ingredients of blood plasmas, those that are against abortion and refuse vaccines due to aborted fetal cells and tissue incorporated in many vaccines should be applaud to go against their Biblically supported conscience. In this video with testimonies from former employee of the CDC, other proof is introduced in exposing the corrupt system lies of the Pharmaceutical Industry. YOU should be very concerned for the health of yourself, your children, grandchildren, and as a majority [not all] nurse and physicians who are accomplices or ignorant in this deadly scheme of corruption and tyranny. The Chair of the U.S. House Intelligence Committeehas pressured Facebook to block conversations about vaccination and Amazon to censor books and videos containing information about vaccine risks and failures. This censorship is also happening with YouTube, Google and Google search engines, and Pinterest. With the Pharmaceutical Industry exempted by the Federal Government from being sued from vaccine or drug injuries, the government National Vaccine Injury Compensation Program: has paid out billions of dollars to those adults and children injured by vaccines. [https://www.hrsa.gov/vaccine-compensation/index.html] Very few people know about this program existing. They do not advertise its existence. Of course the funding is being paid by the taxpayers and not the Pharmaceutical Industry.Tom Armstrong Censorship Is the Last Tool of Tyrants – Dr. Mercolahttps://articles.mercola.com/sites/articles/archive/2019/05/28/vaccine-censorship.aspx?utm_source=dnl&utm_medium=email&utm_content=art1&utm_campaign=20190528Z1&et_cid=DM290835&et_rid=625977365 https://www.noticiasaominuto.com/lifestyle/1258645/nove-alimentos-que-provavelmente-tem-na-cozinha-e-agravam-depressaoNote that aspartame works as a blocker of the production of serotonin. In fact, a recent study shows that aspartame depletes serotonin as much as 74%. https://yournews.com/2018/12/18/792006/bombshell-study-shows-aspartame-depletes-neurotransmitters-in-the-brain/ For information regarding your data privacy, visit Acast.com/privacy See acast.com/privacy for privacy and opt-out information.

Autoimmune diseases are on the rise. There are over 80 different diseases in this catagory. The ingredients in a vaccine, specifically the additives that stimulate the immune response called "Adjuvants". These additives can over stimulate your immune system causing your body to attack itself. This episode we will cover the science behind and the solutions for getting your body out of the hyper-excitable state. Don't miss this life changing episode.

Since 1986 the vaccine companies have had immunity frrom procecution for any damage their vaccines do to the public. This episode we will look into Aluminum in vaccines and see if our government has been asleep at the wheel.  The science is clear that vaccines are linked in a contributing or causal factor in a multitude of chronic diseases and illnesses. Don't miss this life saving and life changing episode!

A Groundbreaking Guide to the HPV Vaccine and the Science, Safety, and Business Behind It Cancer strikes fear in people’s hearts around globe. So the appearance of a vaccine to prevent cancer–as we are assured the human papillomavirus (HPV) vaccine will–seemed like a game-changer. Since 2006, over eighty countries have approved the vaccine, with glowing endorsements from the world’s foremost medical authorities. Bringing in over $2.5 billion in annual sales, the HPV vaccine is a pharmaceutical juggernaut. Yet scandal now engulfs it worldwide. The HPV Vaccine on Trial is a shocking tale, chronicling the global efforts to sell and compel this alleged miracle. Today, two of the book's three authors join THC: Kim Mack Rosenberg & Eileen Iorio

During This episode we bring you a recording of Dr. Raymond Snyder, Harrell’s Director of Agronomy and Dr. Travis Shaddox, Assistant Professor, Turfgrass at the University of Florida discussing the power of adjuvants. They’re covering how adjuvants work, why they are beneficial and also which adjuvant to choose. This conversation was recorded in 2013 when Dr. Shaddox was on the Harrell’s TurPh.Dudes team. The TurPh.Dudes conducted a demonstration that was also recorded as a video. Take a look on the webpage of this episode at www.TurPhDudes.com.

Joel Wipperfurth is joined by guest host Jon Zuk on this episode of The Deal With Yield. The two cover the main factors farmers should consider when selecting and using adjuvants, including a review of current product testing and best practices for in the tank, in the air and in the plant applications. Plus, Jon teaches us a lesson using spaghetti.

HEALTH EMPOWERMENT NEWS WITH CROFT WOODRUFF EPISODE 9 The Health Empowerment Podcast was originally published in 2009. Some of the events referenced in the show are not relevant today but the overall content is still useful. This show is brought to you by The Protandim Resource Page. Protandim is one of the only natural health products […]

HEALTH EMPOWERMENT NEWS WITH CROFT WOODRUFF EPISODE 9 The Health Empowerment Podcast was originally published in 2009. Some of the events referenced in the show are not relevant today but the overall content is still useful. This show is brought to you by The Protandim Resource Page. Protandim is one of the only natural health products […]

Editor's Audio Summary by Howard Bauchner, MD, Editor in Chief of JAMA, the Journal of the American Medical Association, for the July 21, 2015 issue

This week we welcome Dr. Stephanie Seneff, a Senior Research Scientist at MIT's Computer Science and Artificial Intelligence Laboratory, for an in-depth (and sometimes slightly technical) conversation on the subject of glyphosate, the world's most popular herbicide. Marketed as Roundup by Monsanto, and most famously used in combination with Roundup Ready® GMO crops, glyphosate is officially considered to be of minimal risk to human beings when used as directed. But is that really true? After several years of research - drawing upon studies into the effects of glyphosate on animals, and persuasive statistical correlations between the increase in glyphosate usage and the upsurge in various human illnesses, most notably autism - Dr. Seneff reaches a conclusion very different from the official line: Glyphosate, she believes, is a major risk to human health and needs to be removed from our environment as soon as possible. (For show notes please visit http://themindrenewed.com)

This week we welcome Dr. Stephanie Seneff, a Senior Research Scientist at MIT's Computer Science and Artificial Intelligence Laboratory, for an in-depth (and sometimes slightly technical) conversation on the subject of glyphosate, the world's most popular herbicide. Marketed as Roundup by Monsanto, and most famously used in combination with Roundup Ready® GMO crops, glyphosate is officially considered to be of minimal risk to human beings when used as directed. But is that really true? After several years of research - drawing upon studies into the effects of glyphosate on animals, and persuasive statistical correlations between the increase in glyphosate usage and the upsurge in various human illnesses, most notably autism - Dr. Seneff reaches a conclusion very different from the official line: Glyphosate, she believes, is a major risk to human health and needs to be removed from our environment as soon as possible. (For show notes please visit http://themindrenewed.com)

Professor Vincenzo Cerundolo tells us how research in immunology leads to the development of new cancer treatments. Immunotherapy stimulates the bodys immune system and can be a powerful treatment for cancer. With the aim of developing better treatment strategies for cancer patients, Professor Vincenzo Cerundolo is working to gain a better understanding of the cell-to-cell interplay required for optimal expansion and activation of tumour-specific T cell populations.

Professor Vincenzo Cerundolo tells us how research in immunology leads to the development of new cancer treatments. Immunotherapy stimulates the bodys immune system and can be a powerful treatment for cancer. With the aim of developing better treatment strategies for cancer patients, Professor Vincenzo Cerundolo is working to gain a better understanding of the cell-to-cell interplay required for optimal expansion and activation of tumour-specific T cell populations.

Topics:   [7:42] Dow’s Gluten Free Flour [15:27] Adjuvants [18:58] Spinal Decompression, Kettlebell Sport and Olympic Lifting [30:48] Ergogenic Effects of Baking Soda [35:30] Loud Noises While Sleeping [39:05] Piracetam and Smart Drugs [42:27] Aspirin for Colon Cancer Prevention [47:02] Green Tea and Folic Acid Absorption [50:51] 50 Rep Workout

Plastid transformation is a valuable technique for both basic and applied science. In basic science the technique is used to study chloroplast function. Applied approaches deal with the potential of the plastid for the production of medicinal therapeutics, in most cases vaccine antigens coupled to adjuvants. Adjuvants are used for the trans-mucosal delivery of attached cargoes. Cell penetrating peptides (CPPs) emerged as valuable tools for the delivery of cell-impermeable cargoes across cell barriers more than twenty years ago. Although the exact mechanism of CPP penetration of cells is still discussed, the applied value of CPPs is documented in a number of clinical studies. Recently, scientists working in the CPP field launched a call for an alternative expression platform for CPP fusion peptides / proteins. Only a short time before, CPPs were introduced into plant science and some impressive first results, manipulating plant cells from the “outside”, were achieved. The present study aimed at combining the fields of plastid transformation and CPPs from the “inside”. We report the first expression of CPP fusion proteins in a plant, more precisely in the plastid. The approach focused on three aspects of CPP fusion protein expression in the organelle: (A) the principal feasibility of CPP-fusion protein expression in the plastid, (B) the location of CPP fusion proteins in the plant cell upon plastid-based expression and (C) the use of plastids for the manufacture of CPP fusions to provide an alternative to the bacterial expression system. Nine prominent CPPs were employed in three vector series to investigate these aspects. In vector series I the selected CPPs were fused to the fluorescent protein eGFP to provide an optical read-out; in vector series II, the CPPs were fused to Arabidopsis MYB transcription factor PAP1 to provide a biological read-out and in vector series III, two CPPs were fused to the human enzyme PAH to introduce plant-based CPP fusion protein expression. Taken together, the expression of CPP fusion in the plastid turned out to be feasible. Transplastomic plants reached homoplasmy, produced viable seeds and stably inherited the desired trait to their progenies in a maternal fashion. Only low protein accumulation levels were detected. Pleiotropic effects occured at the low protein accumulation levels observed. Localisation of CPP fusion proteins was shown to be restricted to the plastid. An inability of CPP fusion proteins isolated from vector series I to penetrate protoplasts, young plant tissue and human cell lines was revealed. The value of a plastid-based manufacture of CPP fusion proteins for clinical approaches failed to be demonstrated due to low fusion protein accumulation levels. Bottlenecks of the current study are discussed and suggestions are made to provide a framework for future efforts.

Tue, 14 Feb 2012 12:00:00 +0100 https://edoc.ub.uni-muenchen.de/14090/ https://edoc.ub.uni-muenchen.de/14090/1/Muenchmeier_Jan_Niklas.pdf Münchmeier, Jan Niklas ddc:610, ddc:600, Medizinische Fak

New World Order and "World Peace" - Yearly Updated Indoctrination for Children - Britain's "Pilot Projects" Spread Worldwide - Globalism, Laws from United Nations. Post-Democratic Century of Change, Rise of EU as Super-Soviet - Scientific Dictatorship, Public Obey and Serve. Vaccine Contaminants, Adjuvants, Cancer Viruses - WHO's Propaganda Blitz, Lying to the Public, Training the Victim - Julian and Aldous Huxley, Devaluation of Human Life, Depopulation Plans. Domestication of "Inferior" Types, Dependence on "Experts" - Planned Extermination - Cancer and Death Rate now Normal - Public Disbelief of Deadly Intentions. Swine Flu Shot, Mass Refusals - Mandatory Inoculations - No Negotiation with Tyranny--Saying NO - Nigerian Polio Outbreak after Vaccinations. Stanford Psychology Department Experiments, Obedience to Authority - Hiring Half the Poor to Kill the Other Half. Vaccine Exemptions - Doublethink - Hero Worship, Sadomasochists in Positions of Power - Iron Curtain of Bureaucracies - Tyranny = No Choice or Escape. Biowarfare, Immune System Destruction - Human Incubators for Shedding Virus - Flu Vaccine Nasal Mist - Genetically Modified Food - Methods of Sterilization - Use of "the Needle" - Bacteriophages. Gold Shipments to Soviet Union.

--{ Win-Win Situation in Vaccine Creation: "Conflicting Reports from Gods of Vaccine, Is Like Watching Athletes on a Trampoline, May Work, May Not, They Say with a Smirk, Previous Flu Shots Didn't, is that a Quirk? Governments Back Pharma, Quite the Deal, And Terribly Fat from Profits They Steal, They Create New Fast Vaccine from Human Tissue, Don't Ask Where it came from, isn't an Issue, If the Swine Flu goes Killer, it has to Evolve, And Pharma gets More Cash, the Cure to Resolve, Endless Crises are in the Works, Fat Cats Thrive Where Ignorance Lurks" © Alan Watt }-- Profit in Fear Industry, Terrorism, Economic Crash, Coming Pandemics - Docu-dramas, Scary Scenarios - Psychiatry, Everyone is Potential Terrorist, Cameras in Homes. Marx and Darwin, "Genius" Stars are Made - Flu Mutation into "Killer" Virus - Big Pharma at Bilderberger Meeting - Royal-Chartered Corporations. Flu Vaccination always "Wrong Combination" and "May or May Not Work" - SARS Outbreak in Toronto, Doctors on News - Government Contracts, Drug Companies Make $Billions off Vaccines - Loans to Third-World Funded by Taxpayers - Rochester NY, Poland, Volunteers for Vaccine and Drug Trials - Inoculations, Adjuvants, Contradictions, PR. "The Summit" movie (CBC - Communist Broadcasting Corporation), Experimental Vaccines, Canadian PM, Provocateurs. Paralysis, Stroke, Death and Illness from Vaccines - Pharmaceutical Companies Protected by Law. Foundations, Political Agendas - Public Follow Hollywood Actors and Actresses - Sterilization to "Save Planet" - "Stars" say Having Children Not "Eco-Friendly" and is "Selfish". Parroted Slogans and Terminology, "Carbon Footprint" - Totalitarian Regimes, Massive Cons - Gorbachev, New Religion based on Earth Worship. Symbols Used as Language, Egyptian Stelas, Five-Pointed Star, Masonic Ritual, Freemasonry. (Articles: ["Drug groups to reap swine-flu billions" by Andrew Jack (ft.com) - July 20, 2009.] ["Children, elderly and black people preyed on by vaccine companies for first H1N1 'clinical' trials in Rochester, New York" (globalresearch.ca) - July 21, 2009.] ["Rochester Clinical Research - Clinical Trials and Studies" [Rochester Vaccines Trial Volunteer Site] (rcrclinical.com).] ["Northern Ireland refuses to fund swine flu work" (healthcarerepublic.com).] ["The True Face Of Global Warming" (wiseupjournal.com).] ["Why more women are only too happy to stay childless" by Maria Sweeney (independent.ie) - July 15, 2009.]) *Title/Poem and Dialogue Copyrighted Alan Watt - July 21, 2009 (Exempting Music, Literary Quotes, and Callers' Comments)

Ziel dieser Arbeit war es BoHV-1 Doppeldeletionsmutanten zu generieren, zu charakterisieren und bezüglich ihrer Vakzineeignung zu bewerten. Die vorgestellten Studien zur Charakterisierung eines gE und UL49.5 deletierten Virus (BoHV-1-DgEDUL49.5) konnten belegen, dass diese Deletionskombination als letal für BoHV-1 anzusehen ist. Ein UL49.5 deletiertes Virus verhielt sich dagegen funktionell wie ein Virus ohne UL49.5/gM-Komplex. Ähnlich wie bei anderen Alpha-Herpesviren war hier wahrscheinlich der zweite Umhüllungsschritt im Zytoplasma der infizierten Zelle gestört, so dass keine infektiöse Virusnachkommenschaft gebildet wurde. Ein Virus mit Deletionen der Genorte UL49 und gE (BoHV-1-DgEDUL49) zeigte hingegen fast keine Beeinträchtigungen im Hinblick auf die Replikationsfähigkeit. Dagegen war die Ausbreitungsmöglichkeit von Zelle-zu-Zelle (cell-to-cell-spread CTCS) einer solchen Mutante im Vergleich zu den entsprechenden einzel-deletierten Viren erheblich verringert. Da es sich hierbei um eine synergistische Verstärkung gegenüber den Einzelmutanten handelte, wurde geschlussfolgert, dass beide Proteine an derselben Funktionskette des CTCS beteiligt sind. Da die doppelt UL49 und gE deletierte Mutante allerdings weiterhin extrazelluläre, infektiöse Virusnachkommenschaften bildete, ist der CTCS als unabhängige Ausbreitungsform offensichtlich durch andere Funktionen bedingt als der klassische Weg der Virusausschleusung. Hinweise darauf, dass gE auch bei BoHV-1 mit pUL49 interagiert konnten dabei durch Kolokalisationsstudien im Laserscanmikroskop abgeleitet werden. Allerdings ließ keine dieser BoHV-1-Mutanten eine besondere Eignung als Vakzinestamm erkennen. Ein gE und TK doppelt deletiertes Virus zeigte hingegen in vitro kaum veränderte Wachstumseigenschaften verglichen mit dem einfach gEdeletierten Virus. Diese Deletionskombination sollte zudem gegenüber einer Virulenzsteigerung durch Rekombination mit Feldviren unempfindlicher sein, als die derzeit erhältlichen Lebendvakzinen. Zur Steigerung der immunogenen Wirkung des Lebendvirus wurde dieser neue Stamm (BoHV-1DgEDTK) in Kombination mit einem adjuvierenden, nicht viruziden, Blockpolymer eingesetzt. Dieser Ansatz wurde in einem Tierversuch an Kälbern im Vergleich zu der Immunisierung mit dem nicht adjuventierten Lebendvirus getestet. Im Ergebnis dieses Tierversuches schieden die Tiere, welche adjuventiert immunisiert wurden, weniger Virus und zudem für eine kürzere Zeit aus. Dies galt im Vergleich mit den nicht immunisierten Kontrolltieren wie auch im Vergleich mit den Tieren, die allein das Lebendvirus zur Immunisierung appliziert bekommen hatten. Auch die Quantifizierung der neutralisierenden Antikörper verdeutlichte eine gesteigerte Immunogenität der Kombination des doppelt deletierten Lebendvirus mit dem Adjuvants. Die sehr gute Immunitätslage der Tiere nach Impfung führte allerdings nach Belastungsinfektion auch zu einer zeitlich verzögerten Serokonversion im Markertest auf Basis des Nachweises von gE-spezifischen Antikörpern. Auch dies muss als Beleg für die hervorragende, immunisierende Leistung der neuen Präparation angesehen werden, da offensichtlich bei einigen der immunisierten Tiere die Virusreplikation soweit unterdrückt wird, dass keine gEspezifische Antikörperantwort mehr erfolgt. Die vorgestellte Kombination eines genetisch überattenuierten Lebendvirus und die Verstärkung der immunogenen Eigenschaften durch Zusatz eines nicht viruziden, potenten Adjuvants können die Grundlage für eine zukünftige Vakzinestratgie zur Bekämpfung der BoHV-1 oder anderer herpesviraler Infektionskrankheiten bilden.