Podcasts about self renewal

This episode of Big Blend Radio's SOUL DIVING SUNDAY Show with Shelley Whizin celebrates Self-Renewal Day (Feb. 2), a day dedicated to stepping out of our comfort zones and embracing activities that nurture our growth, maturity, and joy. It's an opportunity to take a deep breath, reflect on our lives, and renew ourselves—mind, body, and spirit. Read Shelley's article about Self-Renewal, here: https://blendradioandtv.com/listing/a-call-to-care-for-yourself/  And, check out the new seasonal Big Blend "Soul Diving" digital publication featuring her past articles and podcasts here:  https://online.fliphtml5.com/yhwzg/ylqx/#p=1  SHELLEY WHIZIN is the founder and CEO of the Soul Diving Institute™ and author of "What Do You Bring to the Table? A Savory, Sensory and Inspirational Guide to Living a Yummy Delicious Life." Keep up with Shelley and her coaching programs, courses, and workshops here: https://www.shelleywhizin.com/  Tune in to Big Blend Radio's "Soul Diving Sunday with Shelley" show every first Sunday here: http://tinyurl.com/2bjbectk 

Few Americans have been as consistently critical of Donald Trump's morality than the New York Times and Atlantic columnist Peter Wehner. How to prevent the worst happening, Wehner thus wrote, in his final Atlantic column before the election. So now that the worst has actually happened, how exactly is Wehner - who worked in several Republican administrations - feeling about the future of the American Republic? More optimist than one might. American self-renewal is a wonder of the world, Wehner explained to me, which is why, he believes, we should still be remain cheerful about American democracy.Peter Wehner is a contributing writer at The Atlantic and a senior fellow at the Trinity Forum. His books include The Death of Politics: How to Heal Our Frayed Republic After Trump, City of Man: Religion and Politics in a New Era, which he co-wrote with Michael J. Gerson, and Wealth and Justice: The Morality of Democratic Capitalism. He was formerly a speechwriter for George W. Bush and a senior fellow at the Ethics and Public Policy Center. Wehner is a contributing opinion writer for The New York Times, and his work also appears in publications including The Wall Street Journal, The Washington Post, and National Affairs.Named as one of the "100 most connected men" by GQ magazine, Andrew Keen is amongst the world's best known broadcasters and commentators. In addition to presenting KEEN ON, he is the host of the long-running How To Fix Democracy show. He is also the author of four prescient books about digital technology: CULT OF THE AMATEUR, DIGITAL VERTIGO, THE INTERNET IS NOT THE ANSWER and HOW TO FIX THE FUTURE. Andrew lives in San Francisco, is married to Cassandra Knight, Google's VP of Litigation & Discovery, and has two grown children.Keen On is a reader-supported publication. To receive new posts and support my work, consider becoming a free or paid subscriber. Transcript“What we're called to be in our lives, personally and maybe vocationally, is to be faithful, not necessarily successful. Whether a person is successful in life depends often on circumstances that they can't control. That's just the nature of human existence. But you do have some measure of control of whether you're faithful or not. And that's really what honor is.” -Pete WehnerAK: Hello everybody. Election was two weeks ago, but we're trying to figure out the implications of the Trump/Vance win in the presidential election. We've done a number of shows, one with my old friend Jonathan Rauch. Rauch believes that November 5th represents what he calls a "moral catastrophe." And I'm curious as to what my guest today will say, whether he'll try to trump his old friend John Rauch. Wehner I've always seen as the conscience of American conservatism. He wrote a piece in The Atlantic—he writes a lot both for The Atlantic and The New York Times. Before the election, he wrote a piece for The Atlantic about preventing the worst from happening. He's joining us now two weeks after the election. Pete, did the worst happen? Is it a moral catastrophe?PETE WEHNER: Well, I see the worst happened in terms of what the binary choice was for this this election. Obviously, it's not the worst that could conceivably happen to a country, but given the circumstances, it's the worst that happened. Is it a moral catastrophe? You know, it's a moral blow. And I think it's a moral indictment, actually, of of much of the country as well. Whether it's a moral catastrophe remains to be seen. I mean, events will write that story. But I'm certainly concerned about where we are politically in terms of classical liberalism, in terms of the moral life and moral compass of America.AK: Immediately after the election. Peter Baker, New York Times writer, one of your one of your companions, colleagues on The Times, wrote an interesting piece about Trump's America, suggesting that this is the America who we are. Kamala Harris argued that we were different. But Baker believes that this is the America. It's Trump's America. As you know, Pete, he quoted you in the piece. You said, "This election was a CAT scan on the American people. And as difficult as it is to say, as hard as it is to name, what it revealed, at least in part, is a frightening affinity for a man of borderless corruption." Tell me more about this CAT scan. What does it tell us about the America of late 2024?PETE WEHNER: Well, I think it tells us things that are disturbing. It doesn't mean—and I wouldn't say and I didn't mean to imply—that people who themselves voted for Donald Trump are morally corrupt. But what I do mean to argue is that everybody who voted for Donald Trump voted for a man of borderless corruption, a man of moral depravity. And that's disturbing.AK: It's more than disturbing, Pete, the way you put it. "Moral depravity." In what way is he depraved?PETE WEHNER: Well, let me count the ways. I mean, the man was found liable to sexual assault. He's adulterer, porn star. He's cheated on his taxes and charitable giving. He tried to coerce an ally to find dirt on his opponent. He invited a hostile foreign power in the election. He instigated an insurrection against the Capitol. He tried to urge a violent mob to hang his vice president. He's a man who says racist things. He's a misogynist. He surrounds himself with people who are themselves deeply problematic, including picks that he wants for his cabinet. I would say that corruption has touched every area of his life, personal, professional, and in the presidency. So I don't think that that's a difficult argument to make. I think there's empirical evidence for it. But if there is a counter argument, I'm open to hearing it.AK: Well, I'm certainly not going to make that counter argument. You seem on the one hand, Pete, a little...tentative about, shall we say, morally smearing all Trump voters with his depravity. On the other hand, you know that everybody knows everything about Trump. There are no secrets here.PETE WEHNER: Right.AK: Can one then vote for Trump and not be in any way smeared by this moral depravity?PETE WEHNER: Yeah, it's a good question and I've thought a lot about it, Andrew. The way I think about it is that for Trump supporters, many of them, in any event, look, I know them. I mean, we've friends throughout our life, and I wouldn't deny that you can be a Trump voter and be a wonderful parent or neighbor and a person of high moral quality in a lot of areas in your life. On the other hand, I would say that this was an important election, and that Trump's depravity was undisguised. In fact, he kind of hung a neon light on it. And for an individual to cast a vote for that kind of man, who has done the things that he's done, and he's promised to do the things that he's done, I do think reflects on the person's character. And I don't think it's says everything about a person's character. I don't think this is the most important thing about a person's character. But I do think it says something. And I think that the people who voted for him should at least own up to who he is and the kind of man that that they cast their vote for. So if that's the tentativeness that you hear from me, that's an effort to explain why it's both tentative but something that I have fairly strong convictions on.AK: Pete, you and I talked about this a lot. You've been on the show many times. So it's a wonderful opportunity to talk to you. Is the church/state division in your head as sharp as it should be? For you, is politics essentially an extension of morality? I've always suspected there's an element of that, and I don't necessarily mean that as a criticism. It's just a reality of how you think.PETE WEHNER: Yeah, I don't take it as a criticism. I do think that politics is an extension of of morality. I don't think it's the most important extension of morality. And I do believe that the people who are indifferent to politics, you know, their morality expresses itself in different ways. But yeah, I think from my youngest days, at least in junior high and high school and on, I've always had a sense that politics, at its core, is about justice and the pursuit of justice. And it's about a lot of other things. And it's an imperfect means to achieve justice; there's other ways to achieve justice. But I do think that that's what politics is about. And politics is also the expression of a set of moral beliefs. I mean, that, after all, is what law is in many cases. So I do think that morality and politics are tied. The last point I'll make on it, Andrew, is that if politics goes bad, if it goes really bad, it can have catastrophic human consequences. Gulags and killing fields and genocide and a lot of things less bad than that but that are bad enough. And so I just feel like that matters. And that's certainly a manifestation of morality.AK: What about the argument, Pete, that for all the immorality, the depravity, to use your word, of Trump, most of the voters are voting for change. There's a photo in one of your pieces, I think it may be in the Baker piece, of a Trump supporter on a motorbike with a "Trump 2024" flag, and the suggestion that the rules have changed. It seems to be clear in the two weeks after the election that Trump is determined to change the rules. I mean all his appointments seem to be challenging the current assumptions, institutions, elites, and conventions. Isn't that a good thing? America seems bogged down—I mean, I know you're a conservative, but there were many areas from health care to foreign policy to the environment, and they need to be fundamentally changed. It was a very odd election in the sense that Kamala Harris was supposed to be the progressive, and yet she turned out to be the conservative. She seemed to be suggesting that not much in America needs changing. She didn't seem to want to distance herself too much from Joe Biden, whereas Trump is the candidate of change. Is that a credible argument?PETE WEHNER: No, I don't think it's credible. At least let me qualify that. He's certainly a candidate of change. I think whether it's positive or negative change is really what matters. I think it's one thing to say that institutions need to be reformed, which I agree with and have agreed with for many years and have been part of various efforts, throughout the years, to advocate for the reform of institutions. It's another thing to try and destroy institutions, to burn them down. And I think that Trump and the MAGA world is in the latter category. I think that that is the ethos which defines them. So, you know, in terms of people who voted for Trump out of the country, 50%, whatever, the number is going to end up being, vote for him. I understand the impulse, some of the frustrations that have been expressed. So that is its own topic of conversation, which we can get into. But to me, the idea that Donald Trump is the solution to the problems is not plausible. And I point out too, Andrew, that he did have one term prior to it. And in many respects, the things that people are unhappy about got worse, not better, under his watch. So if you compare what his promises have been to what his record was in the first term, I just don't think it squares. And in addition to that, the kind of things that he's promoting now, I think will make things worse. Just to take one specific area, the manufacturing crisis. There's no question that, for a whole variety of reasons, that there's people who have been in the manufacturing industry have suffered. But actually, it was worse during Trump's watch than it was under Biden's watch. So I don't think that Donald Trump is is the answer to the to the question, even a legitimate question, that's being presented or posed.AK: Pete, you've always described yourself as a conservative. You believe that now you're homeless as a conservative. I wonder what you made, though, of the Harris campaign. Her association with Liz Cheney, of course, represents the conservative wing of the Republican Party that you've been involved with all your life. You work with Cheney and Bush and Reagan. Do you blame Harris for losing the election? Did she make a series of mistakes? And what does it tell us about the Democratic Party? I mean, it's always easy—you've written extensively about the crisis of the Republican Party and its Trump-ification. But is there a similar crisis within the Democratic Party?PETE WEHNER: Well, I think there's a crisis, or at least a challenge, in the Democratic Party, which I'll turn to in a second. I mean, they've they've lost two of the last three elections to Donald Trump. So that is a cause for for self-reflection, for for sure. In terms of the Harris campaign, I'm not as critical as a lot of people are of her. I thought she ran a much better campaign than I thought that she would. It wasn't a perfect campaign by any means, but given the tasks she faced, given her own history, I thought that she did extremely well. And I don't blame her for the loss. I think there were certain intrinsic disadvantages that she had. I mean, she was essentially an incumbent in an election where the impulse for the public was change. Joe Biden's approval rating was 41%. She's going to end up with about 48% of the popular vote. That actually, to me is pretty impressive. The idea that she could have beaten, or have been ten points better, in the popular vote from the Biden approval rating would have been a spectacular achievement. I don't think it was achievable. She made mistakes. She didn't distance herself sufficiently from the Biden administration, but I don't think she ever really could have, because she was vice president. I think that the biggest stage, the biggest moment with the largest audience of all, she absolutely obliterated Donald Trump in the debate. I thought her convention speech was good. I'd sort of graded it at a B plus. I thought the convention itself made a lot of sense. I thought her rallies were very good. She was better on the stump than I thought. She had a huge amount of of energy. I thought she was not so good on interviews. And I think she stumbled at a few points, particularly when she was asked on The View where she differed from Joe Biden. She couldn't come up with anything. I think that she should have been prepared for that.AK: But to put it mildly, I mean, that was the most obvious question that everyone wanted to know. How could she have been so unprepared?PETE WEHNER: Well, I don't know if she was unprepared, I assume—AK: Or unwilling or unable to answer this fundamental question.PETE WEHNER: Yeah, I'm guessing that what was going through her mind, and probably the mind of the people that she spoke with, was that there was still a lot of loyalty to Joe Biden. And so she had to be careful in how far she distanced herself from him and whether that would create some unhappiness among Biden supporters. Secondly, she was vice president. And so there's a plausibility issue here, which is: how much can you separate yourself from a president if you're vice president? That said, look, I think she should have had 2 or 3 things that she could have named. And there was a relatively easy explanation, various explanations she could have offered: look, I believe in learning. When facts change, people change. I think that, you know, in my in my earlier life, I was wrong on certain issues and name what they were, and say that hopefully I've learned from that, I hope to continue to learn. I mean, there are all sorts of ways you could answer that. But look, Andrew, I will say this, too, which is having worked on several campaigns and having observed a lot of them over the decades, it's a lot harder to run as a candidate than people can imagine. And every candidate, no matter how good they are, whether you're Barack Obama or Bill Clinton or Ronald Reagan, have made mistakes. And the prism through which people view it is completely based on whether you win or not. If Trump had lost, you can imagine all of the things that we would say about, you know, really, was it wise to to close the argument talking about the penis size of Arnold Palmer or feigning masturbation with a microphone? I mean, there are there are dozens and dozens of things we would have said.AK: Yeah, I take your point, but of course he didn't. Let's talk about conservatism. You always made the argument—you were on MSNBC recently talking about why Trump is an enemy of conservatism. Is now, shall we say, the Harris wing, which is the center/right of the Democratic Party, which seems to have got into bed, so to speak, with Liz Cheney, are they really the conservatives now in America? I mean, they seem to think that America works pretty well. They always talk about America being American, and we're better than that. Is your conservative Republican Party, has it been swallowed by the Democratic Party?PETE WEHNER: I don't think it's been swallowed by the Democratic Party. And of course, it depends on what aspects of conservatism one is talking about. I would say that given the current constellation of reality in the two main parties in America, that conservatives have a better home in the Democratic Party than the Republican Party right now. But I don't think it's a natural home, and it's certainly not the kind of home that conservatives have been used to in the Republican Party pre-Donald Trump. I'd say the main point in terms of the question you asked is to underscore how fundamentally unconservative the Republican Party, Donald Trump and the MAGA movement, are. You know, there's a line in the movie The Dark Knight, the Batman movie, in which Alfred is talking to Bruce Wayne, and Bruce Wayne is trying to explain the criminal mindset to Alfred. And Alfred is saying, but you don't understand. And here he's talking about the Joker. He says, some people can't be bought, bribed, coerced. Some people just want to watch the world burn. And I think that Donald Trump and the MAGA movement have within them that kind of sensibility. I don't think it's defining to all of them, and I don't think it's completely defining to them. But I think that there is a nihilistic impulse, this effort not to reform, as I said earlier, institutions, but just to burn them to the ground, to take a wrecking ball. But, you know, Matt Gaetz as attorney general, or Pete Hegseth as defense secretary or Tulsi Gabbard as the head of the intelligence agencies, and just, out of anger, grievance, try and destroy them, try and destroy the so-called deep state. That's so fundamentally unconservative, in my estimation, that a conservative couldn't, in good conscience, find a home there. And right now, the alternative is the Democratic Party. And I don't think, on that central question of disposition and temperament, the Democrats are nearly as unconservative, nearly as radical, nearly as revolutionary, as the current-day Republican Party.AK: It all reminds me a little bit of a cowboy movie, The Magnificent Seven (or perhaps the Un-Magnificent Seven.) Talk about a natural party, Pete, but does that really work in American politics, where most African-Americans now vote for a Democratic Party that was in favor of segregation?PETE WEHNER: I'm sorry, say that again.AK: You talk about a natural party. You said, well, conservatives said that the Democrats aren't the natural party of conservatism. But can we use this term convincingly in American politics? After all, most African-Americans vote for the Democratic Party, which was the party of segregation.PETE WEHNER: Yeah, the Democratic Party was the party of segregation. And they changed in the end, you know, it took them longer than it should have. No, I don't think that there's anything, you know, endemic or intrinsic to parties that makes them a natural home to any political movement or political philosophy. Because parties change, circumstances change, coalitions change, the base of a party changes. We've seen that really with the Republican Party. It's just a fundamentally different party than it was in the 80s and 90s and 2000s. And the Democratic Party has changed, and changed in some ways, to the worse. And I think they paid a price for that. I do think that you can take a step back and say, look, over the last 50 years, when you chart the trajectory of the Democratic and Republican Party, there are certain trends that you can see. And so for some period of time, I think that the Democratic and Republican parties were natural homes to certain movements.AK: Is there anything we should celebrate about the election? There were a lot of warnings beforehand that there was going to be a massive gender split, and it didn't turn out to be true. Trump promised that he would get a lot of Hispanic and African-American voters. He got a lot of Hispanic and quite a few African-Americans, especially men. Could one argue that November 5th, 2024 was the first post-identity politics election? Is that something to be encouraged about?PETE WEHNER: Well, in this case, I'd say no, because I think the results of that post-identity politics is going to have really damaging consequences. I see your point, and I do think that to the extent that political parties can't count on certain groups constituencies, that's probably, as a general matter, good. It means you have to go out and earn their vote rather than reflexively rely on them. But as somebody who's been a Trump critic, and who has predicted what four more years under Donald Trump is going to be like, I just think that that overwhelms whatever good that could have come out of it. I suppose I would add, there's one good thing that's come out of this, which is there hasn't been violence. But honestly, I think that's because Donald Trump lost, and the Democratic Party believes in the peaceful transfer of power, and they're not going to do in 2024 what Donald Trump and his supporters did in 2020. I'm glad that's not happening, but I think it is worth reflecting on the fact that violence won't happen because the Democratic Party is the more responsible and civilized party in that respect.AK: How are you doing personally? Trump hasn't been shy to boast about his revengefulness. You've being one of his most articulate critics in The Times, in The Atlantic, certainly from the right, or from traditional conservatism, a very strong moral critic. How are you dealing personally with this situation?PETE WEHNER: You know, I think I'm probably dealing with it better than a lot of people would imagine given my own views on Trump. I think just disposition, temperamentally, I'm not a person who has found politics to be overwhelming or disorienting. I don't want to pretend that it's not a difficult moment, both in terms of what I think it means for the country and for what, as I said earlier, what I think it says about the country. And for somebody who grew up loving America and probably, to some extent, mythologizing America, seeing this happen is difficult. But most of my life and the spirit of my life and is based on my relationships mostly with family and with friends. And those, to me, are the things that really determine what my mood is on any given day or any week. I will say that my wife Cindy and I, in the last two weeks, have really been struck by the number of people that we have heard from who are deeply grieved and fearful of what's happening. We saw somebody a week ago Sunday, and Cindy asked this person, how are you doing? And she burst into tears. She had been abused by her husband. And she said that Donald Trump was a person just like her husband, and she couldn't fathom that America elected him. And we have a friend who's a family therapist, and she said she had spent the week before with sexual abuse victims, and the fact that Trump had been elected and that people in her family were celebrating that...other people who felt like much of what they had given their lives to was shattering. So we've really felt more, I suppose, in a listening mode, in a comforting mode, trying to help people to sort through it. It's different, Andrew, I will say, in my experience and the experience of the people around me, I think, in the country now than it was in 2016. I think 2016 could be argued that that was an aberration, a parenthesis, and I think it's clearly not the case. This is the Trump era, and I think that's hard for a lot of people to come to terms with. Other people are celebrating it. They think that this is wonderful. Donald Trump is, to them, the personification of what they want in a leader and a human being. And now we've got it.AK: Yeah, we will see. You wrote an interesting piece in The Atlantic after the election suggesting that 2024 is different from 2016. It's less shocking, more a confirmation. You wrote an interesting piece in response to what happened, "Don't Give Up on the Truth," in The Atlantic. We are where we are. But there is, if not reason to celebrate, reason to, at least, resist. Are you part of a moral resistance, in some ways, Pete, do you think, to Trump, or at least Trumpism, in America?PETE WEHNER: Yeah, I think that's fair. I think some people who have been critical of Trump are going to dial back their criticism, or they just might find other things to think about or talk about or write about. And I understand that. That's not where I am. I mean, I have to think about what my posture is going to be in the Trump era. That's not clear to me yet. And I think it'll become clear to me as circumstances unfold. But, you know, what I wrote, I believed, and I continue to believe in, and the fact that Donald Trump won the election doesn't allay my concerns, it deepens them. I hope I have enough intellectual independence that if he is different than I think, and if he does things that I agree with, that I'm willing publicly to say that. I tried to do that in the first term. And I hope I can do it in a second term and I hope I'm given reasons to do it, and I hope that my foreboding of what this means for America is wrong. But I can't shake what I believe to be true. And I read the opposite views of mine and critiques of mine and try to understand what I'm getting wrong about Donald Trump. And I may be blinded on this, but I don't think I have been wrong about him. I think all of the things that I've been writing about him since 2015—actually, 2011, and go back to the birther moment—I think they've been validated. And I feel like given my role in life and the outlets that I have, that I can't help but give voice to those concerns. And whether that makes a difference or not, time will tell. It certainly didn't have an impact this time around, that's for sure.“Parties change, circumstances change, coalitions change, the base of a party changes. We've seen that really with the Republican Party. It's just a fundamentally different party than it was in the 80s and 90s and 2000s. And the Democratic Party has changed, and changed in some ways, to the worse. And I think they paid a price for that.” -PWAK: Well, you certainly have a natural home on this show, Pete. And in your excellent Atlantic piece, you talk about the importance of truth telling. You are a truth teller, that goes without saying. What do you think is the most effective way, though, to tell the truth these days? I don't think you're a big social media guy, you're not going on X or Instagram or TikTok. How does one most effectively tell the truth in Trump's America?PETE WEHNER: That's such a good question, Andrew, and a deep one. I'm not sure what the answer is. I think in terms of what each individual has to do, they just have to find within the circumstances of their life the places that they can tell the truth. Some of that just may be with family and friends, maybe in neighborhoods and community groups. It may be in churches. It may be, if you're a writer, in The Atlantic, in The New York Times. You know, I think that what's important in telling the truth is that one does it truthfully. That is, that it corresponds and aligns to reality, that it's rooted in empirical evidence, and that one does not dehumanize in the process. And if you're dealing with a person—for example, in my estimation of Donald Trump and what I do believe is this moral depravity, I just think that is true about him—how do you say that? How do you say that without crossing lines? How do you engage with people who are Trump supporters, as I have, many of them, and to try and point out and argue for my position, and to do so in a way that isn't disrespectful or dehumanizing? Those aren't easy questions. I'm sure I haven't gotten them right. But I think you just try the best you can in the world that you live in to try and give voice to the truth. And probably it helps to look back to others who have faced far more difficult circumstances than we have. I mentioned in my most recent Atlantic essay Solzhenitsyn and Havel who were great dissidents and spoke, in the case of Solzhenitsyn, when the Soviet Union was a country to which he was hostage to, and for Havel, there was a communist movement in Czechoslovakia. And they and so many others, Orwell in a different way, and Jesus in a different way, said that the important thing to do was to speak the truth. It doesn't mean you succeed, necessarily, when you do it, but it's important to do. Times change. Circumstances change. Inflection points can happen. And sometimes speaking the truth can create those moments. And other times when those moments open up, people who spoke the truth have a capacity to shape events in a way that they didn't before that. I should say one interesting example that apposite, maybe, you and your own history knowledge: you take someone like Winston Churchill. And Churchill was the same man in the 30s as he was in the 40s, and in the 30s he was viewed as a social pariah, an alarmist, a kind of ridiculous figure, he had very, very little influence. But events changed, the war came, and all of a sudden Churchill became arguably the greatest person of the 20th century. So there's probably a lesson in that for people who want to be truth tellers.AK: Yeah, I've always thought of you, Pete, as the moral conscience of America, although you've been involved in politics, but I can't imagine you ever running for political office. You talked about Solzhenitsyn and Havel in particular as an activist, as someone who stood up very bravely and indeed humorously to the Russian colonialists in Czechoslovakia or Soviet colonialism. Does the anti-Trump movement need a Havel, a Solzhenitsyn, a Winston Churchill? Seems to be lacking, Harris clearly wasn't. I've always wondered whether Michelle Obama could have been that person. And I know that everyone says, well, she couldn't have run. She doesn't like politics, but maybe she had almost a moral responsibility as an American. But where are we going to get an America? Where are we going to get our Churchill, our Havel, our Solzhenitsyn? All of course, white men. Maybe we need some women, too.PETE WEHNER: Yeah, you know, those are rare people. And it's not a dime a dozen. Yeah, I felt like Liz Cheney was that person in this moment more than Harris, more than others. I think I felt that way about Liz, because there was a cost, there was a very concrete and practical cost, to what she had done. And that, to me, is a sign and a symbol of courage, which is: if you do the right thing when there's a cost to doing the right thing. And I thought her articulation of why she broke with Trump and voted for Harris was extremely powerful. So I'd say of the people in the landscape in American politics right now, Liz Cheney would be supreme for me, but of course, she was tossed out of the Republican Party. She was beaten in a primary. And the Democratic Party's not a natural home for her either. So these are her wilderness years, Churchill had his, I'm not saying that Liz is Churchill, Churchill was Churchill and that's about it. But she showed enormous courage and articulation. I think the fact that for a person of my view, she made such a powerful and persuasive case, and it just didn't win over enough voters. And I think that that's an indictment not of Liz, but I think it's an indictment of an awful lot of voters in America. But that would make sense, because I see the world in a certain way, and the majority of Americans saw it differently. And this is a democracy. And so now we've got Trump and the people who voted for him, and the rest of us get to live with them.AK: Are there hierarchies of morality, Pete? There's a great deal of revisionism now on on Churchill reminding us all that he was an overt racist, a colonialist, a warmonger in some ways, although, of course, we don't use that word in terms of his opposition to Hitler. Trump made that point about Cheney, I mean, in his own vulgar way, but Cheney, of course, was also a warmonger—or, certainly her father was, millions of people—well, certainly hundreds of thousands of people—in the Middle East lost their lives because of catastrophic American wars in the region. Could one argue that Cheney's support for these catastrophic wars are equally immoral, if not more immoral, than Trump's moral transgressions?PETE WEHNER: Yeah, if you believe that narrative, I mean, I think that narrative is flawed. I don't mean that the wars weren't mistaken, but I think the way you framed it is is a caricature. But if you believe that, if you're right and I'm wrong, sure, then, of course. And there is a moral hierarchy. I mean, you know, morality is judged by the actions that you take in the moment that you live and the consequences that they create. And if a person or an individual does an action that creates massive harm and the destruction of human lives, human civilizations, if someone is advocating maliciousness and malevolence on a wide scale, that obviously has to be judged differently than if you lose your temper as a boss or somebody who works for you. So morality is a complicated subject. You also have to take into account, to some degree, the circumstances in which people lived. If you lived in the 14th century, if you lived in the 18th century, if you lived in the 20th century, if you lived in the 21st century, there were different moral standards and moral ethics and moral norms. That doesn't mean, in the case of the American founders, the slave holders, that was a grave sin, and I think probably traditionally on the on the American right, because there's been almost a defecation of the founding fathers, that they've been excused too much for tolerating slavery. Lincoln himself, who I think is the greatest American in history, his history was somewhat spotty. I think he was a magnificent figure. And he grew, but that happens. But just to come back to what you said earlier, if you were to say to me, Liz Cheney versus Donald Trump on any reasonable moral spectrum, I would say that that Liz Cheney has him beat by a country mile, by virtually any metric that you want to judge her and him on.AK: In that excellent Atlantic piece, Pete, you talked about this being a moment where we, and I'm quoting you, we need to guard our souls. But what about for those of us who might not believe in the existence of souls?PETE WEHNER: Yeah. Then I would use a different word.AK: What word would you use?PETE WEHNER: Your inner life, your interior life, your sense of humanity, how you view others. I think most people, whether soul is the word that they use, I think most people aren't strict materialists, or they don't believe in scientism. They believe that there are parts of human life, human existence, human reality that aren't materialistic, that has to do with beauty and esthetics and love and = humanity and caring for the least of these. And, you know, many people that I know that are not believers personify those high virtues, honestly, in ways that are more impressive than people I know who claim to be followers of Jesus. So I use the word soul because I think it speaks to something that is true for human life and human beings. But I understand if you're not a believer that you wouldn't use that term. But I imagine that there's some other term that would get at essentially the same thing, which is your core humanity. What makes you an estimable human being. Compassion, honor, dignity, being a peacemaker, and so forth.AK: You're also more cheerful in the sense that you want to remind everyone that, of course, we want to cultivate hope, humanistic hope. But all this needs to be understood within the historical context. You argue that, in the Atlantic piece, presumably Trump's only going to be around for four years. Things change, there are always party realignments, so, cheer us up, Pete. Why might this just be a blip in the history of humanity rather than the end of it in some way?PETE WEHNER: Yeah. It's not going to be the end of humanity. Even if my most dire warnings are realized. Look, I would say that there can be a kind of catastrophism that happens on all sides and that we need to be careful about it. Life is complicated. Human history is complicated. There are moments of glory and moments of catastrophe and disaster. You know, in the American experience, we had the 1850s that lead up to the Civil War. We had the Civil War. We had the profound difficulties in reconstruction. We had segregation, child labor laws, women can't vote. Just enormous challenges in this country. The first election, really contested election in America between Adams and Jefferson in 1800, was a vicious affair. So, you know, we've we've faced a lot. And that's just America. And, you know, you look at world history, I quote it at the end of my essay, "Don't Give Up on the Truth" in The Atlantic, a speech, one of my favorite speeches, that Bobby Kennedy gave in 1966 at University of Cape Town in South Africa, where he talked about the ripples of hope, and how the ripples of hope can overcome the worst and highest walls of oppression. Now, when Kennedy gave that speech, it was 66. It was at the apex of of apartheid, and eventually apartheid was overthrown, and—AK: Yeah, it's worth repeating the RFK quote, "Each time a man stands up for an ideal, or acts to improve the lot of others, or strikes out against injustice, he sends forth a tiny ripple of hope, and crossing each other from a million different centers of energy and daring, those ripples build a current which can sweep down the mightiest walls of oppression and resistance." Of course, it's particularly resonant given that his son is involved in the Trump administration and is probably not someone you're particularly keen on.PETE WEHNER: No, he's, no pun intended, but I'm not particularly keen on his son. But the father I admired, and I think those words are timeless words. And we shouldn't forget them. Look, the other thing I'd say, Andrew, is that what we're called to be in our lives, personally and maybe vocationally, is to be faithful, not necessarily successful. Whether a person is successful in life depends often on circumstances that they can't control. That's just the nature of human existence. But you do have some measure of control of whether you're faithful or not. And that's really what honor is. I mean, honor is living a life—an imperfect life. We all struggle, we're all fallen, we're all flawed—But trying to advance that. And the other thing I would emphasize again is that human life, human history, the progression of countries, are not straight lines. There's forward and backward, there's zigs, there's zags, inflection points develop, and things change in ways that a person may never anticipate. You mentioned John Rauch earlier, and he and Andrew Sullivan were leading the campaign for same sex marriage. When they started that campaign, especially, Andrew, in 1989, I think he wrote a cover story in The New Republic on the conservative case for gay marriage. Now, if you would have asked either of them in the late 80s, 90s and so forth, whether gay marriage would be prevalent or even be found to be a constitutional right, they would have said that's inconceivable. It couldn't happen. And it happened. Whether you agree or not with same sex marriage, it shows capacity of events to change. And you and I could name a lot of things in which that's happened. So you don't know when those moments come, when those inflection points happen. And I also believe the American capacity for self-renewal is a kind of wonder of the world and that people will—AK: Say that again: American self-renewal is a wonder of the world.PETE WEHNER: Yeah. I think the American capacity for self-renewal is extraordinary. I think it's shown itself throughout history. Again, it's a mixed history, but—AK: But where does that come from, that American self-renewal? Is it a spiritual thing? Is it an economic thing? “I think that what's important in telling the truth is that one does it truthfully. That is, that it corresponds and aligns to reality, that it's rooted in empirical evidence, and that one does not dehumanize in the process.” -PWPETE WEHNER: You know, I'd imagine part of it is part of the American DNA. The things that shape anybody in any country, the factors, the history...there's certainly something, I think it's reasonable to say, in America, about freedom and liberty, that is part of the American character. You know, people could go back and read Tocqueville, which is still relevant to what Americans are like. I think our political history has helped shape us. Civil society has helped shape us. So, you know, each country has a certain kind of a DNA. And I think by and large, America's has been good. So there's history to give you hope, and not just American history. So, I just think you need to keep putting one foot in front of the other. I think you have to call out things that happen that are wrong, immoral or illegal as they as they happen, and hope that over time you bend events enough in your direction. Martin Luther King Junior had that quote, which is pretty well known, about the arc of the moral universe bending toward justice, but that does not—AK: It's not natural, is it? As you suggest, it requires human agency, doesn't bend on its own. Finally, Pete, and you've been very generous, as always, with your time. A lot of comparisons, there always have been, with America and the Roman Republic, this shift into, sort of, decadence. There's also a fashion these days for stoicism. Some of the ideologies or the intellectual movements of the late Roman decadent, not the republic, but imperial Rome. What would you say to people—won't say necessarily Stoics formally, but people who are espousing a kind of stoicism—who will say, "Well, I'm just not going to watch the news for the next four years, Trump doesn't really affect me. I'm just going to ignore him. I'm going to go to sleep for four years, and when I wake up, things will have changed." Do we all need to stay awake? Is the stoical response to essentially ignore the political world, is that healthy in Trump's America?PETE WEHNER: I think some people need to stay awake. You know, it really would depend on the facts and circumstances, Andrew. I mean, if you're an individual who feels overwhelmed by what Trump represents and really can't process it in a very healthy way, and you find your spirit being pulled down and obsessing on him and just, you know, casting shadows over your life, then I'd say, yeah, just to the degree that you can pull the plug. Don't follow, you know, the unfolding events, and attend to your life, your inner life, and the people that you love and care for. On the other hand, if that happens more broadly, and just people shut up and don't speak out, I think that that would be a great tragedy, because I think it's important to speak the truth in its own terms. I think it's important that there are individuals who give voice to what people believe and the moral concerns that they have when they don't have the capacity to do it on a large scale. And as I said, you know, I mentioned earlier, Solzhenitsyn and Havel, and I don't pretend that America is in a situation like the two of them faced. So the challenges and sacrifices that are called on Americans today who are in the so-called resistance isn't comparable to what Solzhenitsyn and Havel and many others have faced. But you need to speak out, and you can't go to sleep. Democracy is, as you said earlier, about human agency. We're not corks in the ocean. We're not fatalistic. We shouldn't be fatalistic. We can create movements and trends and moments and trajectories and moments of and periods of honor and and virtuous chapters in the American story. But they don't happen accidentally. And you can be discouraged, but you've got to stay at it. A friend of mine once said that you could be a theoretical pessimist, but you should be an operational optimist.AK: That's a nice way of putting it. Peter Wehner, I'm not sure about American self-renewal being a wonder of the world, certainly your self-renewal is a wonder of the world. It's wonderful to have you around, and we will be calling on your wisdom, your ethical spirit of resistance against injustice, over the next four years. Keep well, keep safe, Pete, and we will talk again in the not-too-distant future. Thank you so much.PETE WEHNER: Thanks. It's great to be with you, Andrew. This is a public episode. If you'd like to discuss this with other subscribers or get access to bonus episodes, visit keenon.substack.com/subscribe

Danielle and Whitney sit down to discuss Oura Rings, sleep, and how body intelligence shapes how they show up as moms and entrepreneurs. They share their personal “check engine light” signals to reset, tools for a better night's sleep, and how they're approaching self-care through stress management.Some of Danielle & Whitney's favorite podcast episodes on self-care (in both the spiritual and physical realm):The Science of True Happiness with Dawson ChurchRecharging Your Libido with Dr. Jill BlakewayPsychiatry, Psychedelics and Standing in Your Truth, with Ellen Vora, MDChange Your Thoughts, Change Your Life with Dr. Joe DispenzaEat Your Medicine with Dr. Mark Hyman Hosted on Acast. See acast.com/privacy for more information.

Welcome to this latest blog article on Self-Renewal, Personal Transformation, Growth, and Resilience. Blog description: Are you feeling stuck or unfulfilled? Do you want to live your healthiest, happiest life? If yes, then self-renewal might be the answer for you! In this article, we'll explore the concept of self-renewal, why it's important, and the different facets involved in it. Plus, we'll discover the fascinating correlation between bodily cell regeneration and self-renewal. Download the Self-Renewal resource I created to support you on your journey to renewal, transformation, growth, and resilience. The questions I ask in the blog are found in the resource. Use it and share it with a friend. Are you enjoying having the option to listen to the blog article? If yes, let me hear from you. Leave a message on this platform or email me at tonya@yourahalife.com. --- Send in a voice message: https://podcasters.spotify.com/pod/show/yourahalife/message

Our guest today is Ben Springwater. Ben is the founder of Matter, a reading app that pulls everything you want to read into one place with curation, audio, and more. There are constant frustrations with how we get served media, as there's infinite content, and it's harder and harder to separate the good from the bad. Ben is working to solve that problem.  He shares what it's like to build a product like Matter and gives insight into how Matter's team makes decisions around the technical and human challenges that come into play.  An avid reader and content consumer himself, we then asked Ben for some of his favorite recommendations, all linked below. Please enjoy this conversation with Ben Springwater. Ben's Recommended Content:  The Road to Self-Renewal  Basic Income, Not Basic Jobs: Against Hijaking Utopia The Weirdly Enduring Appeal of Weird Al Yankovic For the full show notes, transcript, and links to the best content to learn more, check out the episode page here. ----- Making Media is a property of Colossus, LLC. For more episodes of Making Media, visit joincolossus.com/episodes. Stay up to date on all our podcasts by signing up to Colossus Weekly, our quick dive every Sunday highlighting the top business and investing concepts from our podcasts and the best of what we read that week. Sign up here. Follow us on Twitter: @ReustleMatt | @domcooke | @MakingMediaPod | @JoinColossus  Editing and post-production work for this episode was provided by The Podcast Consultant (https://thepodcastconsultant.com). Show Notes  (00:00) Welcome to Making Media (01:36) The Challenges of Building a Product Like Matter (03:41) The Power of Curation in the Digital Age (08:03) The Future of Recommendation Systems (15:26) The Challenges of Building a User-Centric Product (22:14) The Intersection of Reading and Listening in the Digital Age (25:12) The Business Model of Matter (27:04) Ben's Recommendation #1: The Road to Self-Renewal  (33:14) Ben's Recommendation #2: Basic Income, Not Basic Jobs: Against Hijaking Utopia (42:38) Ben's Recommendation #2: The Weirdly Enduring Appeal of Weird Al Yankovic (51:19) The Importance of Being An Enthusiast (52:06) Debrief Learn more about your ad choices. Visit megaphone.fm/adchoices

Hanna Mikkola, M.D., Ph.D., shares her work in hematopoietic stem cells (HSCs) and their complex renewal process. Her research aims to unravel these cells' behavior in mice and humans, offering potential insights for future medical advancements. Series: "Stem Cell Channel" [Health and Medicine] [Science] [Show ID: 39258]

Hanna Mikkola, M.D., Ph.D., shares her work in hematopoietic stem cells (HSCs) and their complex renewal process. Her research aims to unravel these cells' behavior in mice and humans, offering potential insights for future medical advancements. Series: "Stem Cell Channel" [Health and Medicine] [Science] [Show ID: 39258]

Hanna Mikkola, M.D., Ph.D., shares her work in hematopoietic stem cells (HSCs) and their complex renewal process. Her research aims to unravel these cells' behavior in mice and humans, offering potential insights for future medical advancements. Series: "Stem Cell Channel" [Health and Medicine] [Science] [Show ID: 39258]

Hanna Mikkola, M.D., Ph.D., shares her work in hematopoietic stem cells (HSCs) and their complex renewal process. Her research aims to unravel these cells' behavior in mice and humans, offering potential insights for future medical advancements. Series: "Stem Cell Channel" [Health and Medicine] [Science] [Show ID: 39258]

Hanna Mikkola, M.D., Ph.D., shares her work in hematopoietic stem cells (HSCs) and their complex renewal process. Her research aims to unravel these cells' behavior in mice and humans, offering potential insights for future medical advancements. Series: "Stem Cell Channel" [Health and Medicine] [Science] [Show ID: 39258]

Hanna Mikkola, M.D., Ph.D., shares her work in hematopoietic stem cells (HSCs) and their complex renewal process. Her research aims to unravel these cells' behavior in mice and humans, offering potential insights for future medical advancements. Series: "Stem Cell Channel" [Health and Medicine] [Science] [Show ID: 39258]

Hanna Mikkola, M.D., Ph.D., shares her work in hematopoietic stem cells (HSCs) and their complex renewal process. Her research aims to unravel these cells' behavior in mice and humans, offering potential insights for future medical advancements. Series: "Stem Cell Channel" [Health and Medicine] [Science] [Show ID: 39258]

Join us every week for a new "Wednesday Wisdom" episode from Compete Training Academy co-founder and owner Jordan Delks. Tune in to glean insight you'll need along the journey of life for your mind, body, and spirit! Learn more at https://www.competetrainingacademy.org/ #wednesdaywisdom #basketballtraining #mindset #heartset

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2023.07.04.547746v1?rss=1 Authors: Ly, C. H., Chung, J. D., Nguyen, J. H., Tian, L., Schroeder, J., Knaupp, A. S., Su, S., Trieu, J., Salmi, T. M., Zalcenstein, D., Jabbari, J. S., Boughton, B. A., Cox, A. G., Naik, S. H., Polo, J. M., Ritchie, M. E., Lynch, G. S., Ryall, J. G. Abstract: Skeletal muscle contains a resident population of somatic stem cells capable of both self-renewal and differentiation. The signals that regulate this important decision have yet to be fully elucidated. Here we use metabolomics and mass spectrometry imaging (MSI) to identity a state of localized hyperglycaemia following skeletal muscle injury. We show that committed muscle progenitor cells exhibit an enrichment of glycolytic and TCA cycle genes and that extracellular monosaccharide availability regulates intracellular citrate levels and global histone acetylation. Muscle stem cells exposed to a reduced (or altered) monosaccharide environment demonstrate reduced global histone acetylation and transcription of myogenic determination factors (including myod1). Importantly, reduced monosaccharide availability was linked directly to increased rates of asymmetric division and muscle stem cell self-renewal in regenerating skeletal muscle. Our results reveal an important role for the extracellular metabolic environment in the decision to undergo self-renewal or myogenic commitment during skeletal muscle regeneration. Copy rights belong to original authors. Visit the link for more info Podcast created by Paper Player, LLC

Imagine regenerating your life by removing damaged elements and making room for healthier growth. Sounds intriguing, right? In this captivating episode, we explore this concept of restorative killing, inspired by the teachings of Islam, and discuss how it can lead to self-renewal and self-improvement. Listen in as we dive into the importance of self-reflection and cultivating positive traits such as compassion, honesty, and gratitude during this transformational process.Facing fears and overcoming conflicts is challenging sure, but what if you could transform fear into spiritual fuel for growth? Discover how to tap into your passion, create heart liberation, and mentally fortify yourself in this  deep and insightful conversation. We also discuss connecting with Allah SWT to aid in overcoming internal struggles and releasing what no longer serves us. Don't miss this eye-opening and transformative episode!https://www.islamiclifecoachschool.com/wisdom-wednesdays-free-group-coaching

As Black women, we often find ourselves at the intersection of multiple marginalized identities and are constantly expected to be strong, resilient, and selfless. Join my guests Dr. Deena Brown (Founder, The Leadershift Institute) and Eleanor Ward (Life Strategies Coach, Bigger Fish to Fry Coaching) as we explore the importance of self-renewal for Black women and the ways in which we can empower ourselves to prioritize our own well-being. Join us as we delve into the unique challenges that Black women face and discover the power of self-care and self-renewal in creating a more fulfilling life. Are you looking for your training on how to have your unique self-renewal transformation in a small group setting? Join me for the Soul Renewal Intensive Day. Learn more at https://soulrenewalvipday.com. View guest bios --- Send in a voice message: https://anchor.fm/wakeuphappysis/message

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2023.02.03.527029v1?rss=1 Authors: Kong, W., Wang, H., Zhu, X., Han, X. Abstract: Background: Although mesenchymal stem cells (MSCs) are most commonly used in cell therapy and stem cell research, the mechanism and the locations of their self-renewal are still unknown. Method: Mouse blood was collected, and examined under microscopy. The results were compared with the data of human umbilical cord blood (hUCB) collected 10 years ago. Results: We found that the procedure of self-renewal for the mesenchymal stem cells in mouse blood and hUCB needs at least 5 steps. First, tube-shaped stem cell niches release long segmented materials composed of sand-like particles and semitransparent granules. Second, the sand-like particles and semitransparent granules separate from the segmented materials. Third, each of the individual semitransparent granules releases groups of fusiform-shaped structures that do not stain to H&E. The sizes of the fusiform-shaped structures range from 1 to 100 m in length in mouse blood, but can be 200 m in hUCB. Fourth, the large-sized fusiform structures can directly transform into lineage-restricted cellular structures; the medium-sized fusiform structures fuse or engulf each other to form cellular structures. The cellular structures further acquire membranes from the adjacent nucleated cells. Fifth, the nucleolus appears in the new cellular structures before the nucleus. During all the procedures, the adjacent nucleated mesenchymal cells are must needed. Thus, these newly formed cellular structures will further differentiate into nucleated mesenchymal stem cells. Conclusion: Our findings again provide new evidence that, in physiological conditions, mesenchymal stem cell self-renewal needs several steps to complete, which, however, does not occur by mitotic division. The tube-shaped structures are the niches of the stem cells. Copy rights belong to original authors. Visit the link for more info Podcast created by Paper Player, LLC

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2023.01.24.525321v1?rss=1 Authors: Rundberg Nilsson, A. J., Xian, H., Shalapour, S., Cammenga, J., Karin, M. Abstract: Inflammatory mediators induce emergency myelopoiesis and cycling of adult hematopoietic stem cells (HSCs) through incompletely understood mechanisms. To suppress the unwanted effects of inflammation and preserve its beneficial outcomes, the mechanisms by which inflammation affects hematopoiesis need to be fully elucidated. Rather than focusing on specific inflammatory stimuli, we here investigated the role of transcription factor Interferon (IFN) regulatory factor 1 (IRF1), which receives input from several inflammatory signaling pathways. We identify IRF1 as a master HSC regulator. IRF1 loss impairs HSC self-renewal, increases stress-induced cell cycle activation, and confers apoptosis resistance. Transcriptomic analysis revealed an aged, inflammatory signature devoid of IFN signaling with reduced megakaryocytic/erythroid priming and antigen presentation in IRF1-deficient HSCs. Finally, we conducted IRF1-based AML patient stratification to identify groups with distinct proliferative, survival and differentiation features, overlapping with our murine HSC results. Our findings position IRF1 as a pivotal regulator of HSC preservation and stress-induced responses. Copy rights belong to original authors. Visit the link for more info Podcast created by Paper Player, LLC

Capricorn New Moon I structure.  I ground.  I sustain.   Card 1 – How might I benefit from creating more structure in my life? Card 2 –  What practices might I call upon to encourage grounding? Card 3 – How might I better manage my resources for greater sustainability?   On this episode we explore the invitation of the Capricorn New Moon through ritual, tarot connections and a light guided meditation. In addition, we check-in with Jupiter entering Aries and the end of the Saturn in Aquarius transit.  Then, we are joined for an hour conversation with special guest Lindsay Mann!  Lindsay Mann is an intuitive healing artist, author, and permaculture advocate who cares endlessly about this beautiful planet. We met years ago at Maha Rose in Brooklyn, New York. Since then, I have been staying in touch with their offerings through instagram. They channel clear guidance from other worlds in support of this one. As a healing artist, Lindsay has combined Vedic Meditation, their experience as a Reiki Master, Mediumship, and working in the Akashic Records to connect and communicate with the most profound, subtle energies. In our conversation, titled ‘Vibrational Archives', we discuss life as a Capricorn Sun with a Sagittarius Moon, the significance of the Solstice, the importance of organization and devotion, posting on social media as a spiritual practitioner, the esoteric phrase for Capricorn, and what it means to be a spiritual being having a human experience.  One of the most exciting parts about our conversation:  Lindsay graciously offered to open up the Akashic Records for the Cosmic Cousin's community for the year 2023.  If you're interested in hearing our messages, tune into this week's podcast episode. As an added bonus, on the Capricorn New Moon podcast episode, enjoy Yule song the Holly & the Ivy (with the original pagan lyrics) by Asheville, North Carolina based singer-songwriter Adrianne Blanks. As a long time listener of Cosmic Cousins, Adrianne reached out sharing that the podcast helped her get through a really tough transitional time in her life.   As a collaboration with 13 Asheville collaborators including Trollbinde Jewelry, and Mountain Flower Fantasies who make fantasy gowns, Adrianne recently released a full scale music video for this song.  Offering more of a connection to Earth-based traditions of ancient past, Adrianne recorded this song in hopes to bring comfort to more people who don't fully resonate with the music of the season.  I hope you enjoy! Congratulations to dear our friend Heidi Rose Robbins for creating a new book – Everyday Radiance.  Inspired by Heidi's Moon Notes on Instagram, Everyday Radiance: 365 Zodiac Prompts for Self-Care and Self-Renewal offers a daily dose of astrological encouragement that inspires us all to use the energies available to us in the most conscious, proactive, productive ways we can. (How very Capricorn! Heidi is a Capricorn Moon). Published by Chronicle Books, it is available for pre-sale now! In addition, I wanted to share an update that I am still a part of Heidi Rose Robbin's astrology team.  This week we just launched a new offering!  I, along with the four other soul-centered astrologers on Heidi's team, are offering one-hour private astrology sessions.  This is a great option for those who would like to book a reading with me, but for a shorter amount of time than the offering on my website.  To book, click here and scroll down to the bottom of the page. As we close out 2022, I am reflecting back on all the many collaborations I shared with others in our community.  Thank you to all of those lovely humans and magic makers who joined me in conversation this year on the Cosmic Cousins podcast!  It is truly an honor to know you and to learn from you in this lifetime.  Please check the list below and support these incredible practicioners:  – Spiritual Medium + Multidimensional Mama Mary Grisey    – Brandon Altar & Angel Lopez - Hosts of The Spiritual Gayz Podcast    – Astrologer Monique Ruffin aka the Moon Mama    – Spiritual Mentor Olivia Clementine of Love & Liberation Podcast    – Ellen Fondiler & Heidi Rose Robbins of Chart Your Career Podcast – Somatic Healer Adriana Rizzolo of Body Temple Church    – Sarah Jane Chapman Creator of the Be With Your Body Tarot  – Akashic Records Medium Lindsay Mann of Hey Clarity – Kate Scelsa - Author of Luminary: A Magical Guide to Self-Care   – Somatic Astrologer Renee Sills of Embodied Astrology    – Queer Philly Writer Eryn Johnson - Author of Moon Sign – Magical Mentor Tandy Gutierrez of Unicorn Wellness    – Astrologer Rae Sapp, Owner of The Mountain Astrologer Magazine – Sounder Healer & Kundalin teacehr Pavanjeet – Sound Designers Aaron Pfeiffer & Audzinha - Creators of the song: Venus in Aquarius As always, I am forever grateful for you and our community.  Thank you to those of you who tune into the podcast and read the newsletters!  If you feel called, perhaps you will consider becoming a Patreon Supporter. This helps me as an independent practitioner support my life and work.  ***As a bonus, there is a 2-hour lecture on astrology and the body available for free for Patreon Supporters only.  In addition, right now I am offering both Winter astrology and tarot readings. There's also one more one-on-one mentorship available for this Winter–Spring.  I am wishing you a Happy Capricorn New Moon, and a peaceful closing out to 2022.     OTHER LINKS Podcast: iTunes, Spotify, or Podbean About Jeff Hinshaw Patreon for Cosmic Cousins Astrology Mentorship Astrology Reading Tarot Healing Session Sign-up for Mailing List + Newsletter

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2022.10.31.514562v1?rss=1 Authors: Rieskamp, J. D., Rosado-Burgos, I., Christofi, J. E., Ansar, E., Einstein, D., Walters, A. E., Valentini, V., Bruno, J. P., Kirby, E. D. Abstract: Within the adult mammalian dentate gyrus (DG) of the hippocampus, glutamate stimulates neural stem cell (NSC) self-renewing proliferation, providing a link between adult neurogenesis and local circuit activity. Here, we show that glutamate-induced self-renewal of adult DG NSCs requires glutamate transport via excitatory amino acid transporter 1 (EAAT1) to stimulate lipogenesis. Loss of EAAT1 prevented glutamate-induced self-renewing proliferation of NSCs in vitro and in vivo, with little role evident for canonical glutamate receptors. Transcriptomics and further pathway manipulation revealed that glutamate simulation of NSCs relied on EAAT1 transport-stimulated lipogenesis, likely secondary to intracellular Ca2+ release and glutamate metabolism. Our findings demonstrate a critical, direct role for EAAT1 in stimulating NSCs to support neurogenesis in adulthood, thereby providing insights into a non-canonical mechanism by which NSCs sense and respond their niche. Copy rights belong to original authors. Visit the link for more info Podcast created by Paper Player, LLC

More information can be found at www.socialchangeleaders.net Awaken your spirit to adventure; Hold nothing back, learn to find ease in risk; Soon you will be home in a new rhythm, For your soul senses the world that awaits you. John O'Donahue How do you renew yourself and your life? How do you deal with new rhythms in your personal & professional life? Co-host Traci Warnberg-Lemm shares some of her thoughts about an article she stumbled across about self-renewal and shares some important questions social change leaders should ask themselves when new rhythms show up in life. In this episode you will: Hear how all leaders have times in life where they are in need of self-renewal, clarity or re-inventing Listen to Traci discuss an article by Jordan Kassalow about the power of self-renewal Consider how renewal for social change leaders and social entrepreneurs might require different lenses and questions Ask yourself a few questions that may trigger some new thinking, ideas to help you refresh and renew your role as social change leader In this episode: Social Motion, Traci - Social Motion Article, The Power of Self Renewal

More information can be found at www.socialchangeleaders.net    Awaken your spirit to adventure; Hold nothing back, learn to find ease in risk; Soon you will be home in a new rhythm, For your soul senses the world that awaits you.                                       John O'Donahue   How do you renew yourself and your life? How do you deal with new rhythms in your personal & professional life? Co-host Traci Warnberg-Lemm shares some of her thoughts about an article she stumbled across about self-renewal and shares some important questions social change leaders should ask themselves when new rhythms show up in life.  In this episode you will: Hear how all leaders have times in life where they are in need of self-renewal, clarity or re-inventing Listen to Traci discuss an article by Jordan Kassalow about the power of self-renewal   Consider how renewal for social change leaders and social entrepreneurs might require different lenses and questions  Ask yourself a few questions that may trigger some new thinking, ideas to help you refresh and renew your role as social change leader In this episode:  Social Motion, Traci - Social Motion  Article, The Power of Self Renewal

More information can be found at www.socialchangeleaders.net    Awaken your spirit to adventure; Hold nothing back, learn to find ease in risk; Soon you will be home in a new rhythm, For your soul senses the world that awaits you. - John O'Donahue How do you renew yourself and your life? How do you deal with new rhythms in your personal & professional life? Co-host Traci Warnberg-Lemm shares some of her thoughts about an article she stumbled across about self-renewal and shares some important questions social change leaders should ask themselves when new rhythms show up in life.  In this episode you will: Hear how all leaders have times in life where they are in need of self-renewal, clarity or re-inventing Listen to Traci discuss an article by Jordan Kassalow about the power of self-renewal   Consider how renewal for social change leaders and social entrepreneurs might require different lenses and questions  Ask yourself a few questions that may trigger some new thinking, ideas to help you refresh and renew your role as social change leader In this episode:  Social Motion, Traci - Social Motion  Article, The Power of Self Renewal

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2022.10.21.513182v1?rss=1 Authors: Costa, D. F., Ricci, J. M. B., Rodrigues, M. S., Oliveira, M. A., Doretto, L., Nobrega, R. H. Abstract: This study unravels the roles of TGF-{beta} (Transforming growth factor-{beta}) superfamily signaling pathway in the zebrafish spermatogonial activity (self-renewal vs. differentiation) by combining ex vivo and specific pathway inhibitors approaches. The TGF-{beta} superfamily signaling pathway is subdivided into TGF-{beta} and Bone morphogenetic proteins (BMP) subfamilies, and is ubiquitous among metazoans, regulating several biological processes, including spermatogenesis. In this study, we evaluated the function of the TGF-{beta} and BMP subfamily pathways in the zebrafish spermatogonial niche using A83-01 and DMH1 inhibitors, respectively. Our results showed that A83-01 potentiated the follicle-stimulating hormone (Fsh) effects on zebrafish spermatogenesis, reducing type A undifferentiated spermatogonia and increasing differentiated spermatogonia (type Adiff and type B spermatogonia) after 7 days of culture. In agreement with histomorphometrical data, the mRNA levels of dazl (marker of spermatogonial differentiation) and pro-differentiation growth factors, such as igf3 and insl3, were significantly augmented following A83-01. For the BMP signaling pathway, exposure to DMH1 inhibitor showed opposite effects as compared to TGF-{beta} superfamily signaling pathway inhibitor. Histomorphometrical analysis demonstrated an accumulation of type A undifferentiated spermatogonia, while the frequency of differentiated spermatogonia was significantly reduced following co-treatment of DMH1 with Fsh after 7 days of culture. To support this data, expression analysis revealed that BMP signaling pathway inhibitor also decreased the testicular mRNA levels of dazl, igf3 and insl3 when compared to control incubation (Fsh). In conclusion, our study demonstrated that TGF-{beta} and BMP subfamily pathways exert a role in zebrafish spermatogonial niche with antagonistic functions for the spermatogonia fate. The TGF-{beta} subfamily pathway is involved with spermatogonial self-renewal and inhibition of differentiation, whereas the BMP subfamily pathway promotes spermatogonial differentiation. These findings are not only relevant to understanding stem cell biology, but may also be useful in several in vitro assays, promoting control of self-renewal and differentiation by potentially directing these processes. Copy rights belong to original authors. Visit the link for more info Podcast created by Paper Player, LLC

Must listen --- Send in a voice message: https://anchor.fm/tj698/message

Sam provides a quick overview of the intention and plan for the Self-Renewal podcast. Highlights include an introduction to self-renewal, example domains of application, and a preview of the structure and key themes of the podcast.

What a day! What a hard doggone day! Deacon B shared with all of us some ways to get over ourselves and renew the gift of living in this tough world. Patrick had a rough day and perhaps some of the tips Deacon B shares in this show will help Patrick get back on track. Go ahead...enjoy![The "Pulse" bumper music was composed by Evgeny Kiselevich and used under a purchased royalty-free license from Safe Music List. Sound effects are either public domain (or the original author/creator is not identifiable) or provided under license by: fesliyanstudios.com (paid license) and/or YouTube Studios Library (copyright free).]

Can the experience of betrayal in our lives eventually lead to healing? Today's guest, Dr. Beth Hedva, thinks its so. She's the author of “Betrayal, Trust and Forgiveness: A Guide to Emotional Healing and Self-Renewal”. You'll want to pay attention to the tools she offers. When you think about it, who hasn't been betrayed in one form or another? Website: https://www.hedva.ca/

Can the experience of betrayal in our lives eventually lead to healing? Today’s guest, Dr. Beth Hedra, thinks so. She’s the author of Betrayal, Trust and Forgiveness: A Guide to Emotional Healing and Self-Renewal. You’ll want to pay attention to the tools she offers. When you think about it, who hasn’t been betrayed in one form or another?

Can the experience of betrayal in our lives eventually lead to healing? Today's guest, Dr. Beth Hedva, thinks its so. She's the author of “Betrayal, Trust and Forgiveness: A Guide to Emotional Healing and Self-Renewal”. You'll want to pay attention to the tools she offers. When you think about it, who hasn't been betrayed in one form or another? Website: https://www.hedva.ca/

The opportunity to regroup, reprioritize, renew, and perhaps even re-invent yourself are present as a new season is sprung upon us.TODAY, we discuss Self Renewal! What changes for yourself do you wish to see, AND more importantly, what are you willing to leave behind?Welcoming guest entrepreneur, Ian Monat of #RhythmCBDInfusedSeltzers and #AustinTX #Singersongwriter #JoshKlaus to our discussion table!

Sharpen the Saw is Habit 7 taught in 7 Habits of Highly Effective People. Your family and personal life can be stronger than you ever imagined when we are mindful of our personal growth and renewal. Hear about how this has impacted my life. And get Dr. Trevor Dietrich's final thoughts on this subject.  The Focused Mindset Community Ticket (Free to you!) You will have access to The 7 Habits Download I spoke of in this episode https://thefocusedmindset.ck.page/7395f6dff0 Conversations That Empower is a life changing mini-course that is fun to go through and with easy to practice skills for the entire family. Upgrade your communication skills with Conversations That Empower found at https://www.thefocusedmindset.com/products You Tube: Cher The Focused Mindset Instagram: Cher The Focused Mindset Facebook: Cher The Focused Mindset Facebook Group: Solution Focused Families Linkedin: Cher Kretz Cher”s Blog: www.thefocusedmindset.com/blog-by-cher Head over to https://www.thefocusedmindset.com/contact to ask Cher Kretz a parenting question. Your question may land on the show! Want products for your children that will help them be strong and confident? Check out The Big Life Journal. Please use my link here or on my website to shop and you will be supporting this podcast. https://biglifejournal.com/?aff=1186 Thank you for listening! I'd love to send you my monthly focus letter with free family resources. Get your Free Social and Emotional Learning lessons (SEL) 20+ free SEL solution-focused lessons and songs to teach kindness to kids. It's free when you join the focused mindset community. https://thefocusedmindset.ck.page/3c135261b2 website: https://www.thefocusedmindset.com

Legacy Living with Dr. Gloria BurgessHow do you slow down for rest and renewal? In this week's Legacy Living Show, Dr. Gloria Burgess shares key practices to help you get the renewal you deserve. Listen and be inspired by her message and her amazing voice. You'll want to listen to this podcast over and over!

Episode # 10 with Spiritual Guide Andrew Oser. Andrew Oser is spiritual guide and modern day shaman.  He teaches people how to connect to the authentic true self by connecting with the "Magic Of The Mountain," as he likes to call it. Andrew first came to Mt. Shasta in 1978 and he says, "his heart exploded open." He knew that Mt. Shasta was home and he returned time and time again over the years to tap into the sacred vortex of the mountain receiving all of its gifts and power. Finally he officially moved to Mt. Shasta where he has now been leading spiritual journeys for more than 15 years. Andrew has lead more than 1,100 course and has guided more than 5,100 people. He is a former lawyer who studied at Princeton. He went on to found the Joy Of Light Foundation which has served more than 50,000 children and was honored as a Point Of Light By President Bill Clinton. Andrew left that life for one he says is far richer settling in Mt. Shasta. He said he knew it was home when he first came to the mountain. He says he feels so blessed and empowered in the work he does leading people to discover not only the gifts of the mountain, but to learn through the power of the vortex to tap into their own true authentic selves. You too can join Andrew on a guided tour and learn the power of self-renewal and tapping into the wisdom of nature. We talk about it all in this episode. He shares is wonderful insights into the power of nature, self-renewal, dharma and truly living from a place of authentic freedom and power. Join us on this light filled episode. For more on Andrew Oser or to sign up for one his retreats click here. 

SummaryIf you want to improve yourself and could choose only one trait to begin, you should start with humility. It's called the "mother of all virtues" because it opens the door to all kinds of personal development. But humility is also sorely misunderstood. It isn't just an internal attitude about ourselves, but an outward set of behaviors that people can observe. It's also essential to effective leadership. This episode, we'll be taught by humility expert, Prof. Brad Owens. He's done award-winning research on humility in leaders and has shown that leadership humility is key to getting better engagement, more creativity, and higher functioning teams. Prof. Owens will teach us about the specific ingredients of humility that you can practice and encourage in others. About Our GuestBrad Owens (PhD, University of Washington) is a Professor of Business Ethics in the Marriott School of Management at Brigham Young University. His research has been published in the Academy of Management Journal, Journal of Applied Psychology, Organization Science, Personnel Psychology, Leadership Quarterly, Journal of Management, Journal of Business Ethics, and Public Administration Review. Under the general umbrella of Positive Organizational Scholarship, his research focuses on the impact of leader humility on individuals and teams, ethical leadership, and relational energy. Brad's teaching interests include business ethics, organizational behavior, and leadership. Useful LinksProf. Owens' https://marriott.byu.edu/directory/details?id=33567 (Bio Page) https://www.researchgate.net/profile/Katie-Badura/publication/335078435_Motivation_to_lead_A_meta-analysis_and_distal-proximal_model_of_motivation_and_leadership/links/5f64ee98458515b7cf3eb3e8/Motivation-to-lead-A-meta-analysis-and-distal-proximal-model-of-motivation-and-leadership.pdf ("Motivation to Lead: )A Meta-Analysis and Distal-Proximal Model of Motivation and Leadership.", Journal of Applied Psychology, Volume 105, Pages 331-354, 2020 https://www.sciencedirect.com/science/article/pii/S1048984317301546?via%3Dihub ("How Does Leader Humility Influence Team Performance)? Exploring the Mechanisms of Contagion and Collective Promotion Focus", Academy of Management Journal, Volume 59, Pages 1088-1111, 2016 https://mgt.buffalo.edu/content/dam/mgt/Faculty-and-Research/OHR/CLOE/Documents/humility-shared-leadership-JAP2016.pdf ("Initiating and Utilizing Shared Leadership in Teams: )The Role of Leader Humility, Team Proactive Personality, and Team Performance Capability", Journal of Applied Psychology, Volume 120, 2016 https://www.goodreads.com/book/show/28559430-kant-and-the-ethics-of-humility (Kant and the Ethics of Humility) Jeanine Greenberg argues that we can indeed speak of Aristotelian-style, but still deeply Kantian, virtuous character traits. She proposes moving from focus on action to focus on a person, not leaving the former behind but instead taking it up within a larger, more satisfying Kantian moral theory.  https://www.goodreads.com/book/show/950928.Self_Renewal?ac=1&from_search=true&qid=KdnmsMHXaI&rank=4 (Self-Renewal:) The Individual and the Innovative Society is a book based on why learning is important to creativity and leading. https://www.goodreads.com/book/show/17782487-humility (Humility:) An Unlikely Biography of America's Greatest Virtue https://www.goodreads.com/book/show/1413853.In_the_Heart_of_the_World?ac=1&from_search=true&qid=T9fcEJPpRB&rank=1 (In the Heart of the World) Mother Teresa shares principles of selflessness, forgiveness, compassion, and spiritual Strength. About Merit LeadershipTo learn more about how you can develop ethical skills that turn peril into opportunity, visit http://meritleadership.com (http://meritleadership.com) Pleasant Pictures MusicJoin the https://pleasantpictures.club (Pleasant Pictures Music Club) to get unlimited access to high-quality, royalty-free music for all of your projects. Use the...

In this episode of the Epigenetics Podcast, we caught up with Margaret (“Peggy”) Goodell from Baylor College of Medicine in Houston, Texas to talk about her work on the epigenetic regulation of stem cell self-renewal and differentiation. Dr. Margret Goodell's laboratory focuses on how differentiation and self-renewal is regulated in hematopoietic stem cells (HSC). In the early stages of her research career, however, Dr. Goodell was able to develop a new method to purify stem cells. This method was based on the characteristic of stem cells to pump out the Hoechst dye that was used for the purification. In recent years, the focus of the lab has been to identify how HSCs decide whether to self-renew or differentiate. To get an answer to this question, the lab has performed genome-wide screens to find differentially expressed genes during the decision process. By doing that, they recently found that the DNA methyltransferase 3A (DNMT3A) was highly and specifically expressed in HSCs and that it is required for differentiation. When DNMT3A was knocked out in HSCs, the cell population expanded dramatically and the ability to differentiate was impaired. This finding led to further experiments in this area and to the discovery of so-called DNA methylation canyons in the genome, which are large regions of very low DNA methylation that harbor highly conserved regulator genes. In this episode we discuss how Dr. Peggy Goodell described a new approach to isolate hematopoietic stem cells even though she was not looking for that, how she discovered DNMT3A as an important factor in stem cell decision making, and how she entered and approached new fields of research along the path of her research career.    References M. A. Goodell, K. Brose, … R. C. Mulligan (1996) Isolation and functional properties of murine hematopoietic stem cells that are replicating in vivo (The Journal of Experimental Medicine) DOI: 10.1084/jem.183.4.1797 Shannon McKinney-Freeman, Margaret A. Goodell (2004) Circulating hematopoietic stem cells do not efficiently home to bone marrow during homeostasis (Experimental Hematology) DOI: 10.1016/j.exphem.2004.06.010 Stuart M. Chambers, Chad A. Shaw, … Margaret A. Goodell (2007) Aging hematopoietic stem cells decline in function and exhibit epigenetic dysregulation (PLoS biology) DOI: 10.1371/journal.pbio.0050201 Grant A. Challen, Deqiang Sun, … Margaret A. Goodell (2011) Dnmt3a is essential for hematopoietic stem cell differentiation (Nature Genetics) DOI: 10.1038/ng.1009   Related Episodes Epigenetic Reprogramming During Mammalian Development (Wolf Reik) Effects of DNA Methylation on Chromatin Structure and Transcription (Dirk Schübeler) CpG Islands, DNA Methylation, and Disease (Sir Adrian Bird)   Contact Active Motif on Twitter Epigenetics Podcast on Twitter Active Motif on LinkedIn Active Motif on Facebook Email: podcast@activemotif.com

Happy Thanksgiving, everyone! We got ourselves an amazing podcast/video today and it was all brought to you by the UNIVERSE. Benjamin had recently connected with me through Instagram and so I realized that he had such a voice, a journey, and things that I've been through, too. Born in Colombia but lived in Norway his entire life, it's time for a personal development podcast on my ESL podcast, unlike anything you've ever heard before!Patreon: https://www.patreon.com/arseniosesllearningPodcast on Spotify: https://open.spotify.com/show/7hdzplWx6xB8mhwDJYiP6fPodcast on ListenNote: https://www.listennotes.com/c/778cf3cfd2564ba5b01f693bfebc96de/arsenio-s-esl-podcast/Podcast on CastBox: https://castbox.fm/channel/Arsenio's-ESL-Podcast-id1251433?country=usCalendar (Consultation)- https://calendly.com/arseniobuck/teaching-coaching-for-1-hourCalendar (Patreon Badge Consultation) - https://calendly.com/arseniobuck/teaching-coaching-for-2-5-hourFacebook: https://www.facebook.com/Arseniobuck/?ref=bookmarksYoutube: https://www.youtube.com/channel/UCIzp4EdbJVMhhSnq_0u4ntAWebsite: https://thearseniobuckshow.com/Q & A: ArsenioBuck@icloud.comLinkedIn: https://www.linkedin.com/in/arsenio-buck-9692a6119/Instagram: https://www.instagram.com/thearseniobuckshow/?hl=enBuzz sprout: https://www.buzzsprout.com/165390Twitter: https://twitter.com/ArseniosESLPodSupport the show (https://www.patreon.com/arseniosesllearning)

A day before Thanksgiving, I can honestly say that I'm more level-headed than I've ever been in my life. In harmony. In oneness. In a space that I've never been before with complete contentment and acceptance. This has been the wildest year of my life, and I honestly believe that now I'm vibrationally ready for when everything returns back to normal. Buzzsprout Affiliate Link: https://www.buzzsprout.com/?referrer_id=1242398Patreon: https://www.patreon.com/thearseniobucksperspectiveSpotify - https://open.spotify.com/show/0x39CEN5tHvfRtfZaAMTgQPodcast - https://www.spreaker.com/show/arsenio...Podcast on iTunes - https://itunes.apple.com/us/podcast/t...YouTube - https://www.youtube.com/channel/UCIzp...Facebook - https://www.facebook.com/thearseniobuckshow/Website - https://thearseniobuckshow.com/Q & A - ArsenioBuck@icloud.comLinkedIn - https://www.linkedin.com/in/arsenio-b...Instagram - https://www.instagram.com/thearseniobuckshow/?hl=enThe Arsenio Buck Foundation: https://thearseniobuckshow.com/?p=8676Calendar - https://calendly.com/arseniobuck/45minTwitter: https://twitter.com/ArseniosESLPod

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Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2020.09.20.305144v1?rss=1 Authors: Gupta, B., Errington, A. C., Brabletz, S., Stemmler, M. P., Brabletz, T., Siebzehnrubl, F. A. Abstract: Radial glia-like (RGL) cells persist in the adult mammalian hippocampus where they give rise to new neurons and astrocytes throughout life. Many studies have investigated the process of adult neurogenesis, but factors deciding between neuronal and astroglial fate are incompletely understood. Here, we evaluate the functions of the transcription factor zinc finger E-box binding homeobox 1 (ZEB1) in adult hippocampal RGL cells using a conditional-inducible mouse model. We find that ZEB1 is necessary for self-renewal of active RGL cells as well as for astroglial lineage specification. Genetic deletion of Zeb1 causes differentiation-coupled depletion of RGL cells resulting in an increase of newborn neurons at the expense of newly generated astrocytes. This is due to a shift towards symmetric cell divisions that consume the RGL cell and generate pro-neuronal progenies. We identify ZEB1 as a regulator of stem cell self-renewal and lineage specification in the adult hippocampus. Copy rights belong to original authors. Visit the link for more info

This month on Episode 15 of the Discover CircRes podcast, host Cindy St. Hilaire highlights three featured articles from the July 31 and August 14 issues of Circulation Research. This episode features an in-depth conversation with Drs Venu Venna and Juneyoung Lee from the Department of Neurology at the McGovern Medical School at the University of Texas Health Science Center at Houston regarding their study Gut Microbiota-Derived Short-Chain Fatty Acids Promote Post-Stroke Recovery in Aged Mice. This episode also includes a brief discussion with BCVS Outstanding Early Career Investigator Award competition finalists, Drs Shyam Bansal from Ohio State University, Emmanouil Tampakakis from Johns Hopkins University, and Yang Zhou from the University of Alabama, Birmingham.   Article highlights:   Veys, et al. GLUT1 in Angiogenesis and BBB Integrity   Zhang, et al. Self-Renewal of Local Macrophages Attenuates DiCM   Lerchenmüller, et al.  CITED4 in Cardiac Remodeling     Dr Cindy St. Hilaire:        Hi. Welcome to Discover CircRes, the podcast of the American Heart Association's journal, Circulation Research. I'm your host, Dr Cindy St. Hilaire from the Vascular Medicine Institute at the University of Pittsburgh. Today I'm going to share with you three articles selected from the late July and early August issues of Circulation Research. I'm also excited to share with you my discussions with Drs Venugopal Venna and Juneyoung Lee, who are from the group of Louise McCullough at the University of Texas Health Science Center, regarding their study Gut Microbiota-Derived Short-Chain Fatty Acids Promote Post-Stroke Recovery in Aged Mice. I also speak with the finalists of the BCVS Outstanding Early Career Investigator Award, Shyam Bansal from Ohio State University, Emmanouil Tampakakis from Johns Hopkins University, and Yang Zhou from the University of Alabama, Birmingham. So first the highlights. The first article I'm sharing with you is titled Role of the GLUT1 Glucose Transporter in Postnatal CNS Angiogenesis and Blood-Brain Barrier Integrity. The first author is Koen Veys and the corresponding author is Katrin De Bock from ETH Zurich. The primary energy source for the brain is glucose and the blood vessel endothelial cells which from the blood-brain barrier supplied glucose to the brain via the glucose transporter protein GLUT1. Patients with genetic mutations in GLUT1 have neurological problems, including seizures, movement disorders, and delayed neurological development. Low GLUT1 levels in the blood-brain barrier have also been linked to Alzheimer's disease in humans and have been known to exacerbate the disease in a mouse model. In this study, the group examined the role of GLUT1 in blood-brain barrier endothelial cells in more detail. They found that while structural integrity of the blood-brain barrier remained intact, inhibiting the activity of GLUT1 in newborn mice impaired aspects of normal blood vessel growth in the brain, and inhibiting GLUT1 in adult mice led to progressive neuron loss, behavioral abnormalities, reduced movement, seizures, and signs of inflammation. The results highlight GLUT1's importance in the brain endothelial cells, and the role of GLUT1 in glucose utilization in overall brain function. The second article I want to share with you is titled Self-Maintenance of Cardiac Resident Reparative Macrophages Attenuates Doxorubicin-induced Cardiomyopathy Through the SR-A1-c-Myc Axis. The first authors are Hanwen Zhang, Andi Xu, Xuan Sun, and the corresponding author is Qi Chen and the work was completed at Nanjing Medical University in China. Doxorubicin and it's analogues are commonly used chemotherapeutic agents. However, the use of these drugs is limited by dose-dependent cardiotoxicity. Doxorubicin-induced cardiomyopathy presents with dilated and poorly functioning left ventricle in the absence of abnormal loading conditions. This may induce cardiac systolic dysfunction. Accumulating clinical evidence suggests that inflammation contributes to doxorubicin-induced cardiomyopathy pathogenesis. Several studies suggest that the inhibition of cardiac inflammation can improve cardiac function; however, the underlying mechanisms remain unclear. This group wanted to explore the role of cardiac resident macrophages during doxorubicin-induced cardiomyopathy progression. They found that cardiac resident macrophages were vulnerable to doxorubicin insult but that monocyte-derived macrophages survived. Further, these surviving monocyte-derived macrophages exhibited a proinflammatory phenotype which contributed to impaired cardiac function. Scavenger receptors are expressed on macrophages and help to modulate their inflammatory response. Global, or myeloid-specific deletion of class A1 scavenger receptor, also called SR-A1, inhibited proliferation of resident reparative macrophages and this inhibition exacerbated cardiomyopathy. At the mechanistic level, this group identified that the transcription factor c-Myc mediated the effect of SR-A1 in reparative macrophage proliferation in doxorubicin-induced cardiomyopathy. The last article I want to share with you before we switch to our interviews is titled CITED4 Protects Against Adverse Remodeling in Response to Physiological and Pathological Stress. The first author is Carolin Lerchenmüller and the corresponding author is Anthony Rosenzweig, and they're from Massachusetts General Hospital. Exercise is good for the heart. It increases cardiac mass which is called physiological hypertrophy, which appears to induce cardiac benefits. However, pathological stimuli, such as hypertension and aortic stenosis, can lead to pathological hypertrophy which is associated with adverse outcomes and can lead to heart failure. Cardiac CITED4 is a protein that is induced by exercise and is sufficient to cause physiological hypertrophy and mitigate adverse ventricular remodeling after ischemic injury. However, the role of endogenous CITED4 in response to physiological or pathological stress is unknown. To understand the role of endogenous cardiomyocyte CITED4, this groups generated cardiomyocyte specific knockouts of CITED4. These mice were analyzed at baseline. They were subjected to a swimming protocol which provided physiological stimuli or they underwent transverse aortic constriction, also called TAC, which causes pressure overload and served as the pathological stimulus for heart remodeling. CITED4 knockout mice developed modest cardiac dysfunction and dilation in response to exercise. After TAC, these knockouts developed severe heart failure with left ventricular dilation and impaired cardiomyocyte growth. The study goes on to show that CITED4 protects against pathological cardiac remodeling by regulating mTOR activity and also a network of microRNAs which control cardiomyocyte to fibroblast crosstalk. So for our interview of this episode, I have with me Drs Venugopal Venna and Juneyoung Lee from the Department of Neurology at the McGovern Medical School at the University of Texas Health Science Center at Houston. Today we're going to be discussing their manuscript titled Gut Microbiota-Derived Short-Chain Fatty Acids Promote Post-Stroke Recovery in Aged Mice. Thank you both very much for joining me today. Dr Venu Venna: Thank you Cindy for having us. It's a pleasure. Dr Juneyoung Lee: Yeah, thank you for the opportunity. Dr Cindy St. Hilaire: It's a wonderful paper. Actually I really enjoyed the nice graphical abstract, that really made a good visual of what this papers about, so I encourage everyone to go take a peek at that. Could you introduce yourselves and tell us a little bit about your lab group? Dr Venu Venna: Yeah, sure, I'm Venu Venna, it's my third year at McGovern Medical School UT Health, and we are a part of a large research group in the Department of Neurology here. This is headed by Dr Louise McCullough. She's also a co-corresponding author on this paper. Unfortunately she's not here today, but it's basically her idea and her initiative that led us to drive this huge project. We are very excited to share with you more details today. Dr Juneyoung Lee: Hi, my name is Juneyoung Lee. I'm postdoctoral fellow here and I'm working with Dr McCullough and Dr Venna. Dr Cindy St. Hilaire: So this manuscript is testing the general hypothesis that the gut microbiome can influence stroke recovery but before we dig into the details of your study, can you give us a little bit of background about what the microbiota gut brain axis is? Dr Venu Venna: That's a great question. Thank you for asking that. So recent advances in 16S sequencing, metagenomics, and metabolic analysis lead us to specifically identify the role of gut microbiota. We have... Everybody consists of large number of microbiota in the gut, so particularly the microbiota's role is largely remains unknown, as of now. The recent advances helped us to understand whether it's for communication of the microbiome, how it actually influences our health, and how the metabolites that are released by the microbiota can actually influence the brain-gut. So this is where the concept of microbiota gut-brain axis continues to evolve and we rely on 16S metagenomics, as well as metabolomics to understand if the microbiome itself has a specific role in the stroke recovery and stroke in this paper. Dr Cindy St. Hilaire: Great, so I know that previous research by you specifically, and also you mentioned your fellow corresponding author, Dr Louise McCullough, your prior work has shown that stroke can cause aberrant changes in the gut regarding things like motility, permeability, activation of gut-immune cells. So this to me suggested that aberrant signaling can come from the brain and affect the gut, but your study is kind of now flipping that. You want to ask the question is changing the gut microbiome after the stroke also beneficial? So there's kind of a chicken and egg type conundrum going on. Is there a preceding event, is it the stroke that alters the gut microbiome primarily, or is the gut microbiome maybe deficient in different people and therefore their stroke outcomes are different? Dr Venu Venna: Yeah, I mean this is a very new emerging field and what's very interesting about this is the brain gut microbiota axis, it's a bi-directional axis. In this case, what we think is if we have a stroke, it may actually directly influence the gut. There is a brain-gut axis. At the same time, the changes in the microbiome can actually trigger an inflammatory state where it can actually contribute to the worst stroke outcomes. It's a chicken and egg relationship as you rightly mentioned, but at the same time what is not known is whether if we can simply manipulate the microbiota, can you actually improve the stroke outcomes or can you improve the age associated outcomes? Because what we found in previous studies is age itself causes changes in the microbiome. Dr Cindy St. Hilaire: Interesting, so just being young or old, if you were to compare those microbiomes of old individuals and young individuals, you see differences that are I guess negatively impactful on things like stroke and disease? Dr Venu Venna: That's exactly right, so the more imaging data coming out from the literature, not just our group, but all other groups, on humans and animal studies, do suggest that age itself is associated with changes in the gut microbiome. Dr Cindy St. Hilaire: So the overall goal of this specific study was to determine if replacing the gut microbiota of an older mouse with the microbiota from a younger mouse would help in the recovery after an ischemic stroke. Can you talk about the design of that study and the different aspects that you had to consider when designing these experiments? Dr Venu Venna: Yeah, absolutely. This was a great question. The initial experiments, like what we were trying to do before, was whether we can actually even manipulate the microbiome in an aged animal. In our previous study, what we did is we took a young animal and we transplanted the biome from an aged animal. We used a combination of antibiotics to actually deplete the existing biome and that's what gave us susceptibility to transplant. Once you transplant the biome into a donor from a host, so the biome can actually sustain for quite a bit of time. This gave us an opportunity to study the direct role of microbiome. Later, what we did was we subjected these animals to the stroke and then what we found is when we induced this stroke in an animal that received aged biome, despite being young, the animal that received aged biome, can itself contribute to the worst stroke outcomes and increased mortality. In this follow-up study, what we decided to do was can we even manipulate the microbiome after stroke? So this is particularly important because most of the clinical patients don't come into medical attention until after stroke. Transplanting the microbiome or even manipulating the microbiome after stroke can have a broader clinical relevance. In this particular study we decided to see if we can actually manipulate the microbiome after several days or several hours after the stroke happens. We decided to test if we can wait for three days. This is a particular time where we can actually see the infarcts get mature and all the injury in all groups of animals are same, and then we transplanted the aged animals with the young microbiome. So this gives us an opportunity to actually study the role of microbiome, independent of infarct. Meaning, all animals have a similar degree of injury, so now whatever the beneficial affects you are seeing because of the microbiome transplant, are potentially due to, not because of the size of the injury, because they have a smaller injury they have better recovery, but it's basically because their infarcts are the same and whatever you're seeing is because of the manipulation of the microbiome. Dr Cindy St. Hilaire: Interesting. Juneyoung, would you like to tell me a little bit about what you found then? Using these interesting fecal transplant models, what are the key results that you found in this study? Dr Juneyoung Lee: Great question. As Dr Venna explained, we treated young biome to aged stroke mice, after stroke. We found that young biome contributes to better behavior outcomes and they regulate the immune system in the brain and the gut and increase the short term brain-gut axis in the aged stroke recipient mice. One interesting finding is that we found dominant T-cells, which are very small number of T-cells in the host, but they secrete proinflammatory cytokines which is IL-17. Cytokines exacerbate new inflammation in the brain so if we treat the young biome, we found that the level of proinflammatory cytokine IL-17 decrease cytokines compared to aged biome. Dr Cindy St. Hilaire: You also focused on short-chain fatty acids, SCFA producing bacteria. What is it about these short-chain fatty acids that are beneficial and what are the signaling pathways that you found to be activated or things that were present that helped to promote better stroke recovery? Dr Juneyoung Lee: Short-chain fatty acids are key metabolites produced by bacterial fermentation of dietary fiber in the gut. These are suspected to play an important role in microbiota gut-brain crosstalk. Also, in our previous study we found that young fecal biome has higher levels of short-chain fatty acid compared to aged biome so we think that the short-chain fatty acid has a beneficial role in our mild level stroke. Dr Cindy St. Hilaire: So are you focusing more on identifying the metabolites or trying to move into humans? What do you think the next step of this vein of research is? Dr Venu Venna: So what we think is right now, this is a very interesting and fascinating finding, even for us. We're trying to understanding what other metabolites could be involved and what other ways as you previously asked, what other pathways these bacteria itself are triggering or contributing to actually enhance this recovery, that's what we are seeing from the young microbiome. As a future direction, we are also seeing if this transplant of biome can have a broader therapeutic relevance, meaning is it only specific to the stroke related outcomes or can it be beneficial in large settings of other age relate diseases like what we are seeing, again as I mentioned before like age related diseases such as... Many age related diseases like cognitive dementia, or Parkinson's disease, any neurodegenerative disease. Dr Cindy St. Hilaire: Well thank you, Drs Venu Venna and Juneyoung Lee for joining me today. I really appreciate it and congratulations again on this wonderful story. Dr Venu Venna: Thank you very much for having us, Cindy, and for this work I would like to acknowledge the funding agency. This work is funded by NIH and also the American Heart Association, both for my Scientist Development Grant and also as well as for Juneyoung Lee's postdoctoral fellowship. This funding helped us to perform these highly innovative studies in gut microbiome axis. Dr Cindy St. Hilaire: Wonderful. Yes, well, we love seeing AHA funded research published in AHA journals, so thank you. Right so now we're going to have our interview with the BCVS Outstanding Early Career Investigator Award competition finalists. I have with me today, Shyam Bansal from Ohio State University, Emmanouil Tampakakis from Johns Hopkins University, and Yang Zhou from the University of Alabama, Birmingham. So congratulations to all of you for being recognized for your outstanding science. These topics are great. The timing of T-cells activity in chronic heart failure, sympathetic neuron signaling, circadian genes and cardiomyocyte proliferation, and the identification of a transcription factor that helps promote maturation of reprogrammed cardiomyocytes. So, Dr Bansal, your abstract that's recognized, is titled Novel Inhibitors For Temporal Modulation Of T-lymphocytes During Chronic Heart Failure. Where was this study conducted and where are you now? Dr Shyam Bansal: Right now I'm at Ohio State University. I joined here in July 2019 and I've been setting up my lab. While doing that, we conducted all this work. This work, most of it is done here, and we have been looking to identify certain inhibitors that can be used for T-cell modulation. Dr Cindy St. Hilaire: Excellent so why should I care about T-cells in the heart? And what did you all find in this paper? Dr Shyam Bansal: The right question is why shouldn't you? T-cells are coming out to be involved in almost every chronic disease. We have heard about CAR T-cell therapy. Recently it revolutionized the whole cancer research field. The heart failure and cardiovascular diseases has also been realizing the importance of T-cells. They're important in a way because they are kind of a two-edged blade. They are protective because we need them to initiate those wound healing cascades so the tissue can regain its original function. But then, too much activation of T-cells can be injurious and lead to autoimmune reactions. In 2017 I published a paper during my postdoc with Dr Sumanth Prabhu at UAB where we showed that these T-cells get activated during chronic heart failure. It's a double-sided activation to get activated immediately after injury but then they go down and they come back again, during chronic heart failure. That's where the two-edged blade comes into picture. If you alter these T-cells during this acute phase, during the cardiac infarction, the animals always do worse, right? They are protective because they are needed for wound healing pathways. Dr Cindy St. Hilaire: We can't just stop them at the start, we need to fine tune. Dr Shyam Bansal: Yes. Dr Cindy St. Hilaire: So what's this temporal aspect you looked at? Dr Shyam Bansal: So that's what we found in my postdoc in 2017 paper. If you inhibit these during the chronic phase, in mouse, in rodents, it was whole weeks after infarction, then you can actually stop maladaptive remodeling. You can complete shut it down, it doesn't get better but you at least shut it down completely. We did those studies by using some antibodies, again CD4+ T-cells, and using genetic mouse models. Dr Cindy St. Hilaire: So do you think anything that you found can quickly or soon translate to humans? Dr Shyam Bansal: That's exactly what we did after we came here, right? So we compared what happens during this chronic heart failure, what happens to these T-cells. We identified one molecular pathway that's associated with receptor signaling, being activated in these T-cells. The interesting thing is, these T-cells came from male mice, not from females. Still, they had strong activation of the surge in receptor signaling. We found a drug molecule that can activate another pathway that inhibits this pathway, so indirectly we're able to inhibit this pathway. We did those studies and found that we can actually stop T-cells from getting activated during chronic heart failure and when we do that, this drug can actually, again, inhibit left ventricular remodeling significantly. Dr Cindy St. Hilaire: Wow. Dr Shyam Bansal: And if you give this drug early in myocardial infarction, again, animals died. Dr Cindy St. Hilaire: It's going to be very important to fine tune when that drug could potentially be administered to humans. Dr Shyam Bansal: Yes, and that's the first drug in our knowledge that can actually target specific antigen activated T-cells. Dr Cindy St. Hilaire: Super exciting, well congratulations again. Well done and well earned. Dr Tampakakis, your study is titled Sympathetic Innervation Negatively Regulates Postnatal Cardiomyocyte Proliferation Through Circadian Genes. So where was this conducted and what position are you in now? Dr Emmanouil Tampakakis: This research was conducted at Johns Hopkins University and I'm currently part of the... I'm Assistant Professor within the Division of Cardiology in the School of Medicine. Pretty much for my curiosity and the fact that we know a lot about the role of neurons for adult heart disease but we really don't know what much about neurons are doing at the neonatal stages in heart development. We know at least in preterm babies where the innervation is really affected, some of them do develop changes in their heart geometry, and there might be a role there, plus there is some data to suggest that the autonomic nervous system does manipulate or does affect the neonatal heart regeneration. The role of neurons to me was really intriguing. Dr Cindy St. Hilaire: So this is linking together sympathetic nervous signaling, circadian genes, and postnatal cardiomyocyte proliferation. Why do I care about all these things fitting together? Dr Emmanouil Tampakakis: Yes, so apart from the fact that it's fascinating knowing that each individual organ has in their body its own circadian genes that regulate actually, several functions. Without being affected by the central nervous system and what's happening in the hypothalamus, which to me is really fascinating, is we really don't know much about what actually regulates and synchronizes the circadian cycle of the heart. We, in this study, showing that actually the innervation that happens in neonatal stages, already aaffects how certain genes are circulating within the heart. That appears to be through one of the adrenergic pseudoephedrine way that the sympathetic nerves are secreted. Again, by affecting this, we are showing that there is more proliferation of neonatal cardiomyocytes which can be important for disease at later time points, and we're also showing that if you mess up two specific circadian genes, Period 1 and Period 2, that are transcription regulators, and are some of the masterminds of this phenomenon, you can actually still affect neonatal cardiomyocyte proliferation which can be important for diseases like heart degeneration and whether we're thinking about manipulating other pathways to induce more regeneration and induce healing in the heart. The novelty of our work is we see that there's a link between that cell cycle and the circadian genes, at least at neonatal time points when myocytes proliferate a little more. Dr Cindy St. Hilaire: So neat. Congratulations again, it's a wonderful story and I'm really happy it's being recognized. And Dr Zhou, you're being recognized for your work that's titled TBX20 Activates Cardiac Maturation Gene Programs Promoting Direct Human Cardiac Reprogramming. So where was this study started and where are you now? Dr Yang Zhou: So I started as an Assistant Professor of Biomedical Engineering at UAB in January 2019. Before I moved to Birmingham, I did my postdoc training at the University of North Carolina at Chapel Hill in Dr Li Qian's lab. I basically studied direct cardiac programming which directly convert non-myocyte cell type to the functional cardiomyocytes. I did a lot of work and found the epigenetic barriers and I find that the features of these direct programming cells and almost in the mouse cells, but we know that we have to move that to the human cells, so then when I moved to Birmingham and then I studied the cardio programming from the cells. Dr Cindy St. Hilaire: Excellent, so your study is looking for ways to really kind of push the direct conversion of cardiomyocytes into a more fully differentiated state. Why is that an important question and what did you find in this study? Dr Yang Zhou: Yeah, it is still challenging to gather a functional beating cardiomyocytes from human fibroblast by the direct reprogramming method. We want to get the functional cardiomyocytes to do the cell therapy. Also this method has promise to do the in situ heart regeneration because we use the transcription factors we can inject these factors to the injured heart then directly convert those cardiac fibroblasts into the cardiomyocytes. So we have to study, we have to know how to get the functional work that cardiomyocytes. Dr Cindy St. Hilaire: That is so neat. So really you're hoping to harness those fibroblasts in the heart that everyone kind of ignores because they're not contractile and you're hoping to really take them and transition them to these fully functioning beating cardiomyocytes. Dr Yang Zhou: Right, so we know that the stuff we are coding are very important for the contractility, the myocyte contractility, so we find that a lot of missing protein expression in the current direct programming cells, so my hypothesis is they might be missing key regulators and can promote expression of those coding in the genes. My computational analysis of the transcription data, I find that this T-box, transcription factor Tbx20, that can highly promote those unexpressed genes in the reprogrammed human cardiomyocyte. Dr Cindy St. Hilaire: That's wonderful. Well congratulations again on some excellent work. So I want to ask you all, early career question, you're all within I think the first couple years of starting up your lab, and we're in the midst of a pandemic which means none of us are in the labs. Maybe staff is at reduced numbers, but first, how's it going? And second is, you're kind of still fresh in terms of transitioning. So I'm wondering if there's any one piece of advice that you'd like to share with maybe someone who's in the middle of transitioning or just about to. Or if there's something you wish you knew ahead of time that you'd love to tell your pre-faculty self? Dr Emmanouil Tampakakis: Yeah, I don't know if I can give advice, already I think I'm too junior to do this. I would say that- Dr Cindy St. Hilaire: What, too traumatized? Dr Emmanouil Tampakakis: Maybe. Probably, or will be traumatized, but I would say for me at least, the things that kept me sane during this is my son, who's three and a half years old and lives in a complete different world, so that helps me balance what's happening out there. Dr Cindy St. Hilaire: That's so important. Dr Emmanouil Tampakakis: Probably some good alcohol at the end of the day but both those two things combined actually helped me maintain my sanity. In terms of advice, I would say to try to enjoy science. Try to stay focused and productive and at the end of the day, enjoy what you do. I think that if you are creative and if you like what you do, find the right people to collaborate and work with and you can hope that you will do well. Dr Cindy St. Hilaire: I agree. Excellent advice. Dr Shyam Bansal: I also have two kids, three years and eight years old, so we were at home for two and a half months or so. I think those kids were really helpful in keeping my sanity because the weather was getting better so we had to put in a swing set for them, get some play items and stuff, so they kept me busy. That was good that way. The advice that I will have for junior investigators is be collaborative. Try to see how you can help others because when you help others, others are ready to help you as well. Remember science is a collaborative field, the more you collaborate with people, the more you get to know many more stuff. Dr Cindy St. Hilaire: I think that's so important. Dr Shyam Bansal: I was able to get done a lot of whatever work we presented. I was able to set up my lab, get some work done, and be at a position that I was able to summit my first abstract to BCVS for my independent own work, just because I had good collaborations here. I had good people who helped me stand on my feet and obviously I was working and helping them also. Dr Cindy St. Hilaire: Yeah, and it also sounds like you have good colleagues so that's another key to it. Dr Shyam Bansal: Yeah, I'm really lucky that way. Our whole department is really great. We have several senior faculty who are always ready to help us out in whatever issues we have, personal, professional, scientific, they're always here for us. Dr Cindy St. Hilaire: That's great. Yang, how about you? Dr Yang Zhou: I think the pandemic is very challenging for our junior faculty and for research and career developments but we have to balance between the work and the family, like kids. You might have an issue because we have two PIs in our lab. Dr Cindy St. Hilaire: Oh gosh. Dr Yang Zhou: Yeah, but I learned a lot these two years before this position. I think the most important thing I feel like is you have to talk to people. You always can find people that can help you to find answers. You need a mentor because they are more senior, they have more experience, even in this pandemic, if you find someone to share even just your feelings, that's very helpful. Dr Cindy St. Hilaire: We're almost lucky that this happened now where we can have platforms like Zoom, and Adobe Connect, where we can have these virtual conferences because at least on all the different committees I'm on, ATVB and BCVS, we can have these discussions and break-out sessions, so I think it's really... We're lucky it's happening now and not 1997 when there's no video. Dr Yang Zhou: Concerns... We all feel that the University and the Department, they all responded very quickly and they have much more support here than before. Dr Emmanouil Tampakakis: As a more senior, what advice can you give us as a more senior person? Dr Cindy St. Hilaire: Oh gosh, more senior? Well thank you. My advice? I definitely agree on the collaboration, I think that's key. Finding sponsors is equally important, someone who's going to go to bat for you. Finding a safety net where you can send someone a half-baked game page and have them tell you just how bad it is and be honest and be willing to give you that kind of critical feedback is really important. Building your network is key, and getting involved in societies and getting people to know you independently from your former mentor, I think is really critical. Yes, you want to collaborate but you also got to make sure that you have your own path in the sand, to make sure you can move forward independently, and have fun while you're doing it, like you said. Great, well I wish you all the best of luck. Congratulations again on being recognized and I'll see you on the BCVS webinar. That's it for our highlights from the late July and early August issues from Circulation Research. Thank you for listening. Please check out the Circulation Research Facebook page and follow us on Twitter and on Instagram with the handle @CircRes and #discoverCircRes. Thank you to our guests, Drs Venu Venna and Juneyoung Lee, and to the BCVS Outstanding Early Career Investigator Finalists, Shyam Bansal, Emmanouil Tampakakis, and Yang Zhou. This podcast is produced by Rebecca McTavish and Ishara Ratnayake, edited by Melissa Stoner, and supported by the editorial team at Circulation Research. Some of the copy text for the highlighted articles is provided by Ruth Williams. I'm your host, Dr Cindy St. Hilaire and this is Discover CircRes, your on-the-go source for the most exciting discoveries in basic cardiovascular research.  

This podcast discussions the importance of dedicated self improvement. Find us on Youtube at Motivation Mastery Mind --- This episode is sponsored by · Anchor: The easiest way to make a podcast. https://anchor.fm/app --- Send in a voice message: https://anchor.fm/motivationmind/message

Sign up today for the August 21 Day Carnivore Shred Challenge!https://www.21daycarnivoreshred.com/

On the first of many in this series, Kendall and Angela pull three cards to discuss from an intuitive card deck: Soulful Woman Guidance Cards By Movsessian & Summers. Major themes in this episode include the dangers of hustle culture, listening to your inner answer, Kendall's relationships with goodbyes and revelations in Cuba, Angela's big ask for patience, and more. We hope you enjoy! Angela's Instagram: @angela_party Kendall's Instagram: @kendallhanna

In this episode you'll learn the 3 Habits of Public Victory and the Hbit of Self-Renewal mentioned in the Book 'The 7 Habits Of Highly Effective People' by Stephen R. Covey.

Back on the podcast train, habit slide, establishing a new habit, accountability, self renewal, John Gardner, Dr Rhonda Patrick, acute exercise, benefits of fasting, summertime play, and a Tabata anywhere workout.

Welcome back to Adulting! Self-Renewal is essential to the successful adult life. Let's take some time for some self-assessment.If you're a Iowa, Iowa State, or Northern Iowa student, we would love to get you to connected to a campus minster. ccf.uiowa@gmail.com Feel free to reach out there if you have questions about the show, our organization, or if you have any comments!Music from https://freetouse.comTrack: Vlad Gluschenko - TimeSupport the show (http://paypal.me/CCFofIowa)

Every crisis presents opportunities to learn more, make systems better and develop unimagined relationships. Those opportunities are a pathway to self renewal. It all starts with an intentional positive thought. This uplifting podcast offers 7 ways to self renewal amid the COVID-19 pandemic. 

Awareness of the possibilities of self care are huge in quality of life. We can live so many ways and through so many prisms that we do not realize how many ways of living can be self healing and strengthening to our souls. What are those ways and how did you not hear about this until now? Tune in and join in on the conversation tomorrow morning at 5:30 AM PT/ 8:30 AM ET! 

Conspiracy Theorist extraordinaire Gordo Rochford (of 'Those Conspiracy Guys' podcast) very kindly takes time out of his busy schedule to chat all things human. Gordo tells us what it's been like taking time away from Dublin to live out West and get some coastal air into his lungs. New perspectives have been acquired, self-awareness has been enhanced and personal development is being constructed at an impressive pace! :)  Gordo also shares with us his philosophy on 'The New You', the benefits of spreading good vibes and embracing change: both life and personal. Other topics discussed in this episode include: childhood anxiety, skepticism, being distrustful, questioning, seeking answers, uncertainty, bullying, finding solace and escapism in the liquid hug of alcohol, conspiracy theories, nature, going sober, self-care, stand-up comedy, podcasting, online communities, ambition, living abroad, relationships, new experiences and the wonders of 90s Estonian internet!  Give it a listen, like & share with your fellow humans! :)

Are you interested in Physician Wellness, want to connect with like-minded physicians, go to an academic conference with national academicians and thought leaders in the field and get to know them, AND have fun and relaxation in an amazing resort in Santa Fe, New Mexico? The founders of the Burnout to Brilliance conference which is April 2-5, 2020 discuss their passion for physician wellness, the need for physicians to make a difference for themselves and each other. They also discuss their process and passion for creating this conference, which is in its 4th year, as well as why the B to B conference is different than other physician wellness conferences.   website address: www.burnouttobrilliancecme.com If you mention that you heard about the conference via the podcast, you will receive 100 dollars off of the registration fee. Early registration ends on February 3rd, 2020. The B to B conference is accepting abstracts. More information at www.burnouttobrilliancecme.com There is also a scholarship for residents called the Charles S. DeJohn, MD, PhD Scholarship fund for Physician Education for Wellness and Career Sustainability. More information at www.burnouttobrilliancecme.com

Become conscious of words in your environment because you are bringing them to life in your self-image --- This episode is sponsored by · Anchor: The easiest way to make a podcast. https://anchor.fm/app

I felt called to record a podcast this morning on why it is so important to our highest self to shed our skin and allow for new things to come through. Self Renewal is so important!! Happy Friday guys!!

Self-Renewal for the Motivator - An excerpt from Bringing Out The Best In People by Alan Loy McGinnis

Does the term "self care" make you scratch your head and leave you confused and almost feeling frustrated? When you hear the term, it might make you think of the most selfish, self-centered woman you know...or it may cause you to laugh outloud, because, WHO IN THE WORLD ACTUALLY HAS TIME FOR SELF CARE?! Well, in this episode of Mom on the Fringe, I talk through my own journey in discovering what self care looks like and does not look like for me personally. I share how appealing the secular viewpoint was for me, but also how much the literal interpretation of it threw me into a tailspin and caused me to become an insatiable, unbearable monster who seemed to never get enough ME TIME. Throughout the episode, I share contents of the book "A Mother's Guide to Self Renewal" by Renee Peterson Trudeau, and I compare its contents with that of the book, "Loving the Little Years" by Rachel Jankovic. Spoiler Alert: My head loves the first book, but my heart knows the second one is actually the way I want to live out my years as a mother to 3. Stay tuned for the next episode of Mom on the Fringe! So glad you're here! You can find me and more information about Mom on the Fringe on my website, as well as Facebook and Instagram. Also, if you haven't yet, please jump over to iTunes, SUBSCRIBE to this podcast and leave me a rating and review! It would mean so much to me, and it would really propel this podcast and get the message out to others! Thanks for listening! xo, Amy

As human beings, we have the ability to renew ourselves anytime and to change our circumstances, our livelihood, our direction, and our purpose. We are all here to figure out what that is, and as reflected in mother nature, we all go through our own life cycles and periods of personal transformation to evolve into the person we are born to be. Life has its valleys and peaks, and through the valleys we are supposed to go through suffering for the reward of wisdom and enlightenment in the end. In these moments of lulls or valleys, it’s the time for self-reflection to prepare us for renewal! Spring has sprung and the symbolism of Easter and Passover mean is to remind us of the opportunity to always have a rebirth and renewal in life, whether it’s your love life, your career, purpose, relationships or your self-care, and strengthen the core of who you are. This show gives you the motivation and shares tips to help you renew anything you want to in life and on the road to self-renewal! Connect with Panney Wei at: Instagram: @seethewaywithpanneywei Facebook: www.facebook.com/seethewaywithpanneywei Twitter: @panneywei Website: www.panneywei.com

The Connected Yoga Teacher Podcast 110: Create a Yoga Proposal Package with Lisa Zaehringer   Description:   If you've ever wanted to share or promote a unique yoga class or workshop, I'm sure you have wondered how best to convey your offering to the yoga studio owner, or even where to begin in reaching out to studios. Lisa Zaehringer, after having to go through this process every 2-3 years, shares more about what goes into the yoga proposal packages she sends to yoga studios, as well as her 7-step process for reaching out to studios.   Lisa is a Yoga Alliance registered yoga teacher, whose work as a yoga teacher complements her profession as a mental health counselor. In her yoga business, she supports mothers of all ages as they reconnect with themselves, reclaim rest, and practice compassionate self-discovery. Her unique workshops and courses include the Sacred Motherhood Restorative Practice, Sacred Baby 8 Week Course, and Sacred Pregnancy Weekend Retreat.As part of her transient military lifestyle, Lisa was having to move every few years, and she had to find an authentic, creative way to be able to approach yoga studios in her new hometowns where she had zero credibility - a yoga proposal package. She explains the different elements that make up the package, and also reveals step-by-step, how she reaches out to studios.Whether you're wanting to create a yoga proposal package to get some clarity about your yoga niche, or you just want to be prepared to take advantage of any new opportunities that may arise, this episode is full of great tips you'll be able to use.   Key Takeaways: [3:34] Shannon introduces her guest for this episode - Lisa Zaehringer. [4:39] What is Lisa's background, and what is the work that she does now? [5:18] What prompted Lisa to develop her mini-retreat for mothers, Sacred Motherhood Restorative Practice? [8:09] Shannon and Lisa discuss how she found her purpose in her motherhood journey. [9:29] What does Lisa's workshop look like? [11:49] What motivated Lisa to create her yoga proposal package? [13:25] Lisa shares more about the challenge she faced in getting yoga studios to understand her unique offering. [14:11] What is in the package that Lisa provides? The first element is the Proposal. [16:43] The second piece is the suggested schedule and themes. [18:03] The third document Lisa includes in her package is a personalized flyer. [18:59] The next item in the package is the Sequence of Events. [19:40] Where does Lisa get the images for her package? [20:55] The last piece of the package is A Gift of Gratitude. [22:06] Where does Lisa begin to approach a yoga studio? She walks us through the 7-stage process. [27:32] If multiple places accept Lisa's offering, does she then have to choose between them? [29:40] What stands out is that throughout this process, Lisa is the one who is choosing, and she has been able to do this because of her clarity in her niche. [31:27] Lisa shares some of her struggles around marketing her yoga offering. [33:42] What are Mama Projects? [37:03] Lisa has some final words of wisdom about creating packages for your yoga offering. [40:47] Shannon shares her key takeaways from this interview. Links: Yoga Proposal Package - PDF The Mother's Guide to Self Renewal, by Renee Trudeau Wild: From Lost to Found on the Pacific Crest Trail, by Cheryl Strayed Canva Sacred Living Movement Sacred Pregnancy A Return to Love, by Marianne Williamson Define Your Yoga Niche Online Workshop The Connected Yoga Teacher Podcast Episode 096: How to Create a Yoga Practice Habit with Matt Kowald The Connected Yoga Teacher Podcast Episode 064: Getting Hired by a Yoga Studio with Kristen Sweeney   Gratitude to our Sponsor Schedulicity   Quotes from this episode:   "I believe that that's where connection happens - when vulnerability is met with compassion."   "I wanted to find an authentic, creative way to be able to approach yoga studios in the new hometown where I had zero credibility."   "There's some doubt and fear that bubbles up each time [I approach a yoga studio], like, is anybody going to get this? Is this going to make sense to anybody?"   "[It's] just those little things that say I really care about the time you're putting into reading this."   "Where ever this is meant to happen, is where it's going to happen."   "The universe is always conspiring in our favor."   "I felt like it's a mutual interview, so I'm interviewing [the yoga studios] as much as they're interviewing me."  

The concept of self-renewal may be the most important idea of all when it comes to becoming more productive and successful long term. Life is a marathon, not a sprint. So unless there is a continuous and ongoing attempt at self-evaluation and self-renewal you may not even reach the finish line before dropping out of the race.

The concept of self-renewal may be the most important idea of all when it comes to becoming more productive and successful long term. Life is a marathon, not a sprint. So unless there is a continuous and ongoing attempt at self-evaluation and self-renewal you may not even reach the finish line before dropping out of […]

Lecture by Swami Tyagananda, given on May 6th, 2018, at the Ramakrishna Vedanta Society, Boston, MA.

HOST BIO:  Linda Stephens-Jones is a Certified Christian Life Coach. Her portfolio includes clients in 21 states & two other countries. Her specialty is helping women to overcome fears & navigate through major life change/challenge i.e. divorce, loss, parental care, career/retirement, or self-doubt. Her coaching results in clients taking action to live with more clarity, confidence & fulfillment!  Two of Linda's transformational, most popular coaching packages are "Six Inspired Choices" and "Six Steps to Self-Renewal", both are faith-based coaching.  To get further information, or sign up for a complimentary self-discovery session with Coach Linda, see: www.lindastephensjones.com  GUEST BIO: Eric Twiggs is a professional speaker who has delivered training and motivational speeches to help businesses & individuals realize their goals by unleashing their unlimited potential. He has shared this inspirational message for professional and personal success with companies, associations, and congregations across the country.  After graduating from Hampton University with a degree in marketing, Eric began his career in the automotive industry as a top producing service advisor, quickly rising into management.  Eric's success resulted in him being promoted to District Manager with responsibility for over $50 million in annual revenue and 500+ employees.  Eric's specialty is time management and he is known as Your Procrastination Prevention Partner.  He will inspire you to maximize your time, minimize your stress, and break the habit of procrastination so that you operate in your Divine calling. He is the author of The Discipline of Now, 12 Practical Principles To Overcome Procrastination. Visit Eric online at www.ericmtwiggs.com or call 1-888-812-4705.                  

In today's episode of Mum-to-Mum Monday we'll be finding out how to re-balance our busy lives as mums with the use of self-care. It's so very important, because when a woman feels alive and whole and connected to her essence, she's unstoppable. My guest today is SuperMum, Renée Trudeau. Renée lives in Austin, Texas with her husband and teenage son and is an internationally-recognised life balance coach and speaker. She' also the president of Career Strategists, an award-winning coaching and consulting firm and her corporate clients include Fortune 500 companies such as Dell, Ernst & Young and IBM. Her work has been featured in the New York Times, US News and World Report, Good Housekeeping, Spirituality & Health and more. She's also the author of three best-selling books on life-balance including The Mother's Guide to Self-Renewal: How to Reclaim, Rejuvenate and Re-Balance Your Life which we'll be talking about today. Renée has been creating and facilitating transformational events for women for more than 20 years. She and her team have trained hundreds of RTA-Certified Facilitators in more than 10 countries around the world to lead self-renewal groups for women based on her award-winning curriculum. Renée has a kind and beautiful soul and genuinely cares about the needs of mothers, so she is the perfect person to set us on the road to self-renewal. I'm overjoyed to have Renée my show today, because as a sought after life balance expert and highly acclaimed author, we can learn so much from her. You can find the full write up from this episode including the Show Notes at www.Lisa-York.com/025 Go Conquer Motherhood!

Elise Manolakos is a fitness coach and lifestyle entrepreneur who has an extensive background in fitness training, running, endurance athletics and much more. She is also a world traveler and retreat host who is now offering monthly Self-Renewal retreats close to the city of Toronto. I actually met Elise through a group in Toronto called Toronto Entrepreneurs of Passion and Purpose or TEPP for short. In this episode, we chat exercise, recovery tips, traveling the world, building and connecting to a community, and much more. If you're a woman and in the Toronto area please do check out her Retreats and enjoy an intimate time of self-care, sharing, and connection to nature. Full post with links availabe at: http://bryanhardy.ca/embitious Outro Song: The Story of You by Satsang

Elise Manolakos is a fitness coach and lifestyle entrepreneur who has an extensive background in fitness training, running, endurance athletics and much more. She is also a world traveler and retreat host who is now offering monthly Self-Renewal retreats close to the city of Toronto. I actually met Elise through a group in Toronto called Toronto Entrepreneurs of Passion and Purpose or TEPP for short. In this episode, we chat exercise, recovery tips, traveling the world, building and connecting to a community, and much more. If you're a woman and in the Toronto area please do check out her Retreats and enjoy an intimate time of self-care, sharing, and connection to nature. Full post with links availabe at: http://bryanhardy.ca/embitious Outro Song: The Story of You by Satsang

While it is easy to focus all of our attention on our fragile baby, we will be unable to care for them if we do not first take care of ourselves. Guests include Renee Trudeau, author of The Mother's Guide to Self-Renewal and Kelli Foulkrod, psychotherapist and yoga teacher in Austin, TX, specializing in pregnancy and postpartum mood disorders.    

While it is easy to focus all of our attention on our fragile baby, we will be unable to care for them if we do not first take care of ourselves. Guests include Renee Trudeau, author of The Mother’s Guide to Self-Renewal and Kelli Foulkrod, psychotherapist and yoga teacher in Austin, TX, specializing in pregnancy and postpartum mood disorders.    

A discussion on creating a future of joy, insight, and purpose. What does it really take, to create a yogic life? https://www.youtube.com/watch?v=qJUMSBWThgs

Lecture by Swami Tyagananda given on September 14, 2014, at the Ramakrishna Vedanta Society, Boston, MA

Lecture by Swami Tyagananda, given on Jan 5, 2014, at the Ramakrishna Vedanta Society, MA

Dr Hans Clevers talks to ecancer at ECC 2013 about comparing colon cancer cells to healthy stem cells and combining the study to discover the process where cells become cancer cells. Dr Clevers discusses how using an inducible Cre knock-in allele and the Rosa26-LacZ reporter strain, lineage tracing experiments were performed in adult mice. The Lgr5 ve crypt base columnar cells (CBC) generated all epithelial lineages throughout life, implying that it represents the stem cell of the small intestine and colon. Similar observations were made in hair follicles and stomach epithelium. In the future this discovery could lead to drug development that would target this transformation.

How do you renew yourself when you are burned out? We've all been there... worked to the bone, weary, tired, spent. Do you just keep on going until you crash? Learn the art of self-renewal and master refueling on good energy with Master Life Coach and Business Consultant, Alexis Robin.

Die vorliegende Arbeit hatte zum Ziel, die prinzipielle Fragestellung zu beantworten, ob Stammzellen aus humanen Haarfollikeln in ausreichender Menge expandiert werden können und inwieweit eine Differenzierung in neuroendokrine Zellen möglich ist. Es sollte eine Methode zur Isolierung und Langzeitkultivierung von Haarfollikelstammzellen etabliert und optimiert werden, um eine neue Quelle autologer adulter Stammzellen für zelltherapeutische Ansätze zu gewinnen. Durch Verwendung verschiedener Medien und Beschichtungsarten wurde ein Protokoll entwickelt, aus Dispase-verdauten Hautbiopsien Progenitorzellen zu isolieren. Die auf diese Weise expandierten Zellen wurden mit FACS-Analyse, RT-PCR und Immunhistologie charakterisiert. Im letzten Teil der Arbeit wurde durch Zugabe von spezifischen Faktoren die Fähigkeit zur Differenzierung in unterschiedliche Zelltypen untersucht. Nach Austestung verschiedener Zellkulturbedingungen wurde eine neue Population von Zellen aus der Haarfollikelregion isoliert. Diese Zellen, die als hBSCs bezeichnet wurden, waren über mehr als 30 Passagen mit stabilem Phänotyp kultivierbar (Self-Renewal). Zudem waren sie im Colony-Unit-Assay positiv und zeigten die Expression pluripotenter (Oct4) und multipotenter Stammzellmarker (Nestin, BCRP1, Sox2). Die molekulare Signatur der hBSCs zeigt einige Übereinstimmung mit Merkelzellen, neuroektodermalen Zellen der Haut, die eine Rolle als Mechanorezeptoren und neurosekretorische Zellen der Haut spielen. Unter Verwendung von etablierten Protokollen wurde die Fähigkeit der Differenzierung in Adipozyten, Osteoblasten, glatte Muskelzellen, Neuronen und endokrine Zellen untersucht. Der Nachweis der Entwicklung von glatten Muskelzellen, Neuronen und in eingeschränktem Maße auch Adipozyten belegt die Multipotenz der hBSCs. Darüber hinaus besitzen die hBSCs die Fähigkeit, stimulusabhängig Somatostatin zu exprimieren und zu sezernieren. Somit ist es erstmals gelungen, humane adulte Stammzellen/Progenitorzellen mit neuroendokrinen Eigenschaften zu isolieren. Zusammenfassend ist es in der vorliegenden Arbeit gelungen, eine Methode zu etablieren, mittels derer eine neue Population von humanen multipotenten Stammzellen aus der Haarfollikelregion in Langzeitkultur expandiert werden konnte. Die Plastizität der hBSCs und insbesondere die Differenzierung in reife endokrine Zellen muss in weiteren Studien untersucht werden.

We continue our ongoing series of interviews with "Courageous Parents, Confident Kids" book contributors as podcast host Amy Tiemann talks to Renee Trudeau about the absolutely essential need for mothers to practice self-care. Renee has written extensively about this issue in her book "The Mother's Guide to Self-Renewal," and her training that teaches women how to form and facilitate their own Personal Renewal Groups.  Amy has also written about self-care as a core principle in her book "Mojo Mom:  Nurturing Your Self While Raising a Family."  So these two have a lot to talk about! Listen in to find out why mothers in particular have a hard time claiming their own self care as a top priority,  why it's really important to learn how to do so, and how to get started.  Then make sure you register on MojoMom.com to reserve a free digital download of the new book that is a collaboration between  Amy, Renee, and 12 other experts, "Courageous Parents, Confident Kids--Letting Go So You Both Can Grow."  Sign up now and we'll send you a free digital download of the new book when it's released on April 19, 2010.

Happy New Year! Today’s podcast was the result of a wonderful experiment at Deer Park.  Zen Teacher Thay Phap Hai and two Order of Interbeing members, Karen Hilsberg and Kenley Neufeld, shared a dharma talk on self-renewal.  These wonderful teachers offer insights into how we can begin anew with ourselves and practice right away by watering our own flower.  We can also reflect on our relationships and our lives and find new places to be open to experience and to life.  With these insights we can set our intentions for the new year.   We hope you enjoy your flowering watering and have a joyous and happy new year.  

Would you like to reconnect with who you are? Do you desire a life or meaning and connection? Would you like to be part of a Personal Renewal Group? Join us for answers to these and other questions as Renee Trudeau highlights her book, The Mother’s Guide to Self-Renewal. Completing our family focus month on Spiritual Parenting in Unity, we celebrate the sacred call to motherhood.

Michael Clarke lectures on stem cell self-renewal when dealing with cancer. (September 17, 2007)

Click To Play play_blip_movie_451699(); Renee Trudeau, author of "The Mother's Guide to Self-Renewal", shows you how to overcome obstacles and turn them into business opportunities. She also explains the importance of patience; the process of turning your dream into a business organically takes time.

Click To Play play_blip_movie_416696(); Renee Trudeau, from Career Strategists, started a new project out of her need for support when she had her son. This project has now evolved into a new business, support groups all over the country, and a complimentary work book, "The Mother's Guide to Self-Renewal".

Click To Play play_blip_movie_416696(); Renee Trudeau, from Career Strategists, started a new project out of her need for support when she had her son. This project has now evolved into a new business, support groups all over the country, and a complimentary work book, "The Mother's Guide to Self-Renewal".

The craziness of the end of the school year has begun, and Amy & Sheryl are doing their best to ride that wave. This is a good time to talk about making self-care, even in the midst of chaos, so Amy talks with Renee Trudeau, author of "The Mother's Guide to Self-Renewal." You can learn more about Renee's 'Personal Renewal Group' programs by visiting her website, www.ReneeTrudeau.com

Hydra interstitial stem cells continuously give rise to daughter stem cells as well as precursors for nerve and nematocyte differentiation. Growth of the stem cell population is controlled by the self-renewal probability (Ps): Ps is the fraction of stem cell daughters that remain stem cells in each generation. We have determined Ps for Hydra interstitial stem cells by using a novel technique based on the cell conposition of clones. Stem cell clones were grown in aggregates of nitrogen mustard-inactivated Hydra tissue. They contain several hundred cells after 14 days of growth, including stem cells, differentiating nematocytes, and differentiating nerve cells. Clone size, size variability, and the ratio of differentiating cells to stem cells are sensitive measures of Ps. We have prepared standard curves relating these parameters to Ps, using computer simulations of clone growth. Comparisoon of the experimentally observed parameter of clones to these curves indicates that Ps decreases from 0.8 in 5- to 6-day clones to 0.6 in 10- to 12-day clones. The decrease in Ps coincides with the increase in clone size and suggest that Ps may be regulated by the density of stem cells in clones. Such a mechanism could be responsible for the observed homeostasis of stem cell populations in vivo.