Podcasts about potentiation

In dieser Folge unterhalten sich Christoph Zinner und Sebastian Fischer über das sogenannte Post-Activation-Potentation, auf Deutsch: Post-Aktivierungs-Potenzierung, oder kurz: PAP. Während es diese Warm-Up-Methode schon seit den 70ern gibt, finden sich erst in den letzten Jahren vermehrt empirische Belege zu ihrer Wirksamkeit - auch im Schwimmen. Christoph und Sebastian stellen die Grundlagen dieser Methode sowie einige aktuelle wissenschaftliche Erkenntnisse vor und geben bereits erste Tipps für die Anwendung. In einer zukünftigen weiteren Folge wird es dann um die konkrete Umsetzung von PAP sowie um PAPE gehen, dem sogenannten Post Activation Performance Enhancement.Die schriftliche Zusammenfassung der im Podcast genannten Studien finden sich hier: https://www.scienceforsport.com/post-activation-potentiation/

Join licensed clinical social worker Doug Scott as he delves into an enlightening discussion on the 'Law of Three' - a principle that transcends traditional problem-solving methodologies by engaging consciously with tension and transformation. With a unique blend of spirituality and psychology, Doug provides an in-depth presentation that promises to guide listeners toward deeper connections with themselves, others, and the divine. Drawing from his vast experience, Doug simplifies this complex subject for beginners while incorporating rich storytelling, such as the journey of Emily and Marcus, a couple learning to navigate and heal from intergenerational trauma and spiritual misalignment. Their story showcases how confronting contrasts, tensions, and resolutions can spiral growth and harmony in relationships significantly. This episode invites listeners to move beyond cycles of conflict, embracing an evolutionary spiral of development through conscious awareness. Expect to explore practical applications, including resolving conflicts by locating ambivalence and identifying shared virtues and values. Discover the 'Law of Three' as an avenue for meaningful personal and spiritual development, transforming life's inevitable challenges into growth opportunities. Dive into a transformative exploration that intertwines ancient wisdom with contemporary understanding, perfect for anyone seeking to enrich their personal and relational lives. --- Summary of Key Themes from "How Practical Knowledge of the Law of Three Can Transform Your Relationships" The Law of Three as a Universal Pattern Doug Scott presents the Law of Three (also called the principle of teleopotentiation) as a fundamental pattern woven into the fabric of reality. This pattern describes how all meaningful development occurs through a three-phase process: Every contrast creates potential for tension Every tension creates potential for resolution Every resolution creates potential for a higher-level contrast This creates not a circle but a spiral pattern of growth, where each completed cycle leads to a more sophisticated level of experience. The concept parallels what Cynthia Bourgeault describes in Christian terms as "affirming force," "denying force," and "reconciling force" that together generate a "fourth" or "new arising" - something genuinely new that emerges from their interaction. Teleopotentiation: Purpose-Driven Growth Scott emphasizes that this process is neither random nor predetermined but guided by what he calls a "telos" or "polar north" - a guiding purpose that orients growth. The term "teleopotentiation" combines: "Teleo" (from Greek "telos" meaning purpose, end, or aim) "Potentiation" (the process of enhancing potential or power) This purpose-driven aspect is crucial - transformation isn't just about change but about development toward increasing harmony, complexity, and integration while maintaining balance. Levels of Consciousness and Tension A significant theme is how our level of consciousness affects our engagement with the Law of Three process: When tension remains in the unconscious, we cannot engage with it constructively. We may remain unaware of it even while others can see it (denial). When tension rises to the subconscious, we feel it but may not acknowledge it fully. This creates an "itch that cannot be scratched" and often leads to projection and scapegoating. Only when tension is brought to conscious awareness can we work with it purposefully to create resolutions that lead to growth. Resolution Pathways: Higher Order vs. Status Quo Scott distinguishes between two types of resolution: Bridge to Higher-Order Contrast - When we engage with tension consciously, guided by our "polar north" values, resolution becomes a pathway to more sophisticated development. Bridge to Status-Quo Contrast - When we remain unconscious or focused only on what we're against, resolution merely perpetuates existing patterns. The difference between these pathways isn't about eliminating tension but about how we engage with it. Midwifing Resolution: Practical Approaches Scott offers practical guidance for "midwifing resolution": Locate ambivalence - Recognize that every contrast creates ambivalence (values on both sides). Name both the desire for change and the fear of what might be lost. Locate polar north - Identify highest values and purposes to provide orientation. Ask "What is the goal here? What do you want?" Name the ambivalence - Acknowledge feeling trapped between important values ("I feel trapped between my need for X and my fear of Y"). Discover virtues - Uncover subconscious values by asking what virtues one hopes would be recognized at their funeral. This cultivates hope and provides direction. Bring tension to consciousness - Work to bring unconscious or subconscious tensions into conscious awareness where they can be engaged with purposefully. Application to Relationships The narrative of Emily and Marcus illustrates how the Law of Three operates in relationships: Their contrasting wounds (controlling perfectionism vs. emotional withdrawal) created tension in their relationship Initially, they projected blame and remained in unconscious patterns, creating cycles of pain Their breakthrough came when they identified a shared "polar north" (creating a relationship unlike those they witnessed growing up) They learned to recognize ambivalence, name tensions, and become "midwives" for each other's growth Their resolution opened the way to a "new arising" (their daughter Sophia) that represented a transcendent fourth dimension beyond their individual stories Application to Spiritual Development The spiritual application of the Law of Three is emphasized through: Parallels to Christian concepts of death, tomb time, and resurrection (deconstruction, uncertainty, reconstruction) Recognition that our images of God often unconsciously project our parental relationships The insight that healing human relationships provides a pathway to healing divine relationships Understanding that spiritual growth often requires passing through disorienting phases before reaching more expansive understanding The Transformational Promise The presentation concludes with the promise that understanding and applying the Law of Three enables: Transformation of pain rather than transmission of pain Movement from unconscious reactivity to conscious growth Creation of a legacy of awareness, intention, and love that shapes generations Finding meaning and purpose in life's inevitable tensions and contrasts The central message is that the Law of Three isn't a technique to eliminate life's challenges but a wisdom tradition that helps us engage with those challenges in ways that foster growth rather than stagnation, connection rather than division, and transformation rather than merely coping or surviving.

In this episode of the I Can't Sleep Podcast, drift off while learning about long-term potentiation. I read every word in this recording and still have no clue what it's about. Even after editing, I almost convinced myself I learned something the second time through. Nope! This one's a brain-melter, folks. I can't remember who suggested this topic, but I hope it's your area of expertise and that you can explain it to everyone in simpler terms. Happy sleeping!

In this episode of the J3U Podcast, John and Luke tackle the post-show decision-making process. Should you be blasting gear or should you be coming off of everything? Is there a middle ground? This episode is great for anyone about to compete or who has recently competed to make sure you can face the next stage head-on with full efficiency. Timestamps: Intro 00:00 Goal Setting Post Show 06:12 Health Implications 14:41 Getting Back to Baseline 19:07 Natural to Enhanced 24:22 Understanding Where You Are 33:12

Should Personal Trainers Stretch Clients? Potentiation plays role

Subscribe to Mack's Memo • Intuitive Public Radio on Substack for community resource access, success celebrations, & bonus features: https://intuitivepublicradio.substack.com/p/sol-luckmans-regenetics-dna-potentiation --- Support this podcast: https://podcasters.spotify.com/pod/show/intuitive/support

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2023.07.19.549725v1?rss=1 Authors: Parkins, E. V., Brager, D. H., Rymer, J. K., Burwinkel, J. M., Rojas, D., Tiwari, D., Hu, Y.-C., Gross, C. Abstract: MicroRNAs are an emerging class of synaptic regulators. These small noncoding RNAs post-transcriptionally regulate gene expression, thereby altering neuronal pathways and shaping cell-to-cell communication. Their ability to rapidly alter gene expression and target multiple pathways makes them interesting candidates in the study of synaptic plasticity. Here, we demonstrate that the proconvulsive microRNA miR-324-5p regulates excitatory synapse structure and function in the hippocampus of mice. Both Mir324 knockout (KO) and miR-324-5p antagomir treatment significantly reduce dendritic spine density in the hippocampal CA1 subregion, and Mir324 KO, but not miR-324-5p antagomir treatment, shift dendritic spine morphology, reducing the proportion of thin, 'unstable' spines. Western blot and quantitative Real-Time PCR revealed changes in protein and mRNA levels for potassium channels, cytoskeletal components, and synaptic markers, including MAP2 and Kv4.2, which are essential for long-term potentiation (LTP). In line with these findings, slice electrophysiology revealed that LTP is severely impaired in Mir324 KO mice, while baseline excitatory activity remains unchanged. Overall, this study demonstrates that miR-324-5p regulates dendritic spine density, morphology, and plasticity in the hippocampus, potentially via multiple cytoskeletal and synaptic modulators. Copy rights belong to original authors. Visit the link for more info Podcast created by Paper Player, LLC

In this episode of the David Grey Rehab podcast, David dives into the topic of French contrast training and its application in both training and rehab. He shares his insights and experiences with this training method and offers valuable tips for incorporating it into your own practice. Whether you're a coach, therapist, or fitness enthusiast, this episode is packed with useful information to enhance your understanding of contrast training. _____[00:00:00] Introduction[00:01:30] Using contrast training in rehab. [00:04:08] French contrast training. [00:09:23] Potentiation and its myth. [00:12:21] Secrets in training methods. [00:17:13] Being tired is a skill. [00:21:38] Band assisted jumping. [00:23:14] Hip-dominant exercises in rehab. [00:28:49] Mid stance specific exercises. [00:31:01] Fatigue contrast and pain. [00:34:25] Training under fatigue. [00:38:05] French contrast training._____Connect with David Grey Rehab:Check out our Foot & Achilles Rehab ProgramVisit our websiteFollow David Grey Rehab on InstagramSubscribe to our YT & listen to more podcast episodes Visit the DGR Interactive platform https://youtu.be/UdcmWOFnAPc

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2023.06.23.544496v1?rss=1 Authors: Sirbu, A., Bathe-Peters, M., Inoue, A., Lohse, M., Annibale, P. Abstract: G protein-coupled receptors conformational landscape can be reportedly affected by their local, microscopic interactions within the cell plasma membrane. A pleiotropic stimulus to alter the cortical environment within intact cells, namely osmotic swelling, is employed here to monitor the response in terms of receptor function and downstream signaling. We observe that in osmotically swollen cells the beta2-Adrenergic receptor, a prototypical GPCR, favors an active conformation, resulting in cAMP transient responses to adrenergic stimulation that have increased amplitude. The results are validated in primary cell types such as adult cardiomyocytes, a relevant model where swelling occurs upon ischemia-reperfusion injury. Our results suggest that receptors function is finely modulated by their biophysical context, and specifically that osmotic swelling acts as a potentiator of downstream signaling, not only for the beta2-Adrenergic receptor, but also for other receptors, hinting at a more general regulatory mechanism. Copy rights belong to original authors. Visit the link for more info Podcast created by Paper Player, LLC

What are potentiation sets?!  How can they help you get better results?!  Great questions!!!  Listen in to learn the answers and how you can achieve greater results with one easy concept!!!

Perhaps the two most important words in programming are: it depends. We often focus on the exercise or structure, forgetting about the context. How do you train before a competition? It depends. Are speed ladders good or bad? It depends. Are kettlebells or dumbbells better? It depends. On this week's episode we talk about variety of training topics and try to answer the question of what it depends on. For more information on this topic, read the complete show notes at: https://www.hmmrmedia.com/2023/03/hmmr-podcast-episode-296-it-depends/ »  Support the show: join HMMR PLUS to get full access to our coaching resources. More notes: This episode is brought to you by HMMR Plus. Become a member for full access to our videos, articles, and podcast archives. We discussed pre-meet training and workouts in the recent GAINcast 263. Hex bar training was discussed on this month's Episode 295 with Scott Russell. "It depends" was also covered last year on GAINcast 245.

MINDFULNESS IS BOTH A SCIENCE AND A HABITKevin Bailey is back for his last round with Jordan and in this episode, the two talk about mental health as part of the early stage of the educational system, the pain caused by craving neurotransmitters, the science of creating habits, the best qualities of leaders, and what success means to him. So tune in now to this powerful and packed episode of Peak Performance Selling. PEAK PERFORMANCE HIGHLIGHTS:KEVIN: IT TAKES MORE THAN JUST A FEW DAYS“You're not going to feel gratitude in the first few days, it's going to take a month, it's worth it, you're not gonna get a meditation in a few days, you're gonna be so bombarded with thoughts, you're gonna want to shoot yourself. Give it a few months. I know it's hard. But the only way to believe this stuff is to experience it for yourself.”KEVIN: EMOTIONS HELP MAKE HABITS“The more emotional I get about the practice, the faster it'll become a habit.”KEVIN: DEFINITION OF SUCCESS“The ability to experience joy regardless of what's happening externally, to not shut that down when things go sideways, to be able to just stay in a state of joy, stay in a state of comfort even when things get really uncomfortable normally, like success looks like that to me” You can connect with Kevin and check out his work in the links below:Kevin Bailey | Dreamfuel | Dreamfuel.com If you're listening to the Peak Performance Selling Podcast, please subscribe, share, and send us your feedback.Jordan Benjamin | MyCoreOs.com | Podcast | Email | Twitter

IT'S TIME FOR SOME CHEMICAL ROMANCEKevin Bailey is back for round 3 with Jordan, and this episode is packed. Jordan and Kevin start off with the attitude of gratitude, followed by the mindset of positivity. Last but not the least, Kevin discusses how your emotions are tied to your physiology, diving deep into the different natural chemicals in our bodies that affect the types of emotions we have, and how he coaches people based on their modes or moods. Learn more in this latest episode of Peak Performance Selling. PEAK PERFORMANCE HIGHLIGHTS:JORDAN: FEED YOUR LIFE WITH POSITIVITY“As we've grown to keep ourselves safe, so and so we've got to be afraid of everything. But if we can feed in additional positivity to make that the most efficient path to making that the strongest path, we can now see the world and find those opportunities to send a nice text to see the beauty of the world around us”KEVIN: VISUALIZE IT AND YOU'LL BELIEVE IT“Sometimes it doesn't know the difference in what's real and what is imagined. That's the other thing people don't understand. Visualize it and you'll believe it. The thing that controls 95% of your thoughts, the subconscious mind will believe it and just get the heck out of the way so you can sell.” You can connect with Kevin and check out his work in the links below:Kevin Bailey | Dreamfuel | Dreamfuel.com If you're listening to the Peak Performance Selling Podcast, please subscribe, share, and send us your feedback.Jordan Benjamin | MyCoreOs.com | Podcast | Email | Twitter

SOMETIMES, WHAT YOU NEED TO DO TO FIX YOURSELF IS TO JUST SIT AND RELAXKevin Bailey is back for part 2 with Jordan in today's episode. Kevin talks about the power of meditation and what it really does. He also breaks down the science involved in meditation and how it really works in your body. Kevin and Jordan will also be touching on topics around mental fitness, gratitude, and self-love. Find out more in this latest episode of Peak Performance Selling.PEAK PERFORMANCE HIGHLIGHTS:KEVIN: JUST SIT THERE AND WATCH“the only real good way of becoming mindful of the thoughts that are going through your head is to watch them for a few hours, and there's no better way to watch than just to sit there and watch them, and that's what meditation does. You'll become acutely aware of the stuff you tell yourself.”KEVIN: IT'S ALL ABOUT POTENTIATION“Meditation is really about deep potentiation. That other part, letting go of beliefs that don't serve us. It's not the exciting part of building new beliefs. So I look at meditation as something normally I don't use in the sales process, I kind of like save it for later, but I emphasize how drastically important it is.” You can connect with Kevin and check out his work in the links below:Kevin Bailey | Dreamfuel | Dreamfuel.comIf you're listening to the Peak Performance Selling Podcast, please subscribe, share, and send us your feedback.Jordan Benjamin | MyCoreOs.com | Podcast | Email | Twitter

BUILD A MINDSET STRONGER THAN STRESS AND BURN-OUTJoin us today as Jordan welcomes Kevin Bailey. Kevin is the Co-Founder and CEO of Dreamfuel, which helps teams perform at higher levels while reducing stress. The two discuss burnout, stress, and the importance of focusing on life's quota over the standard sales quota. Kevin also breaks down the 3 ways to create new mindsets so make sure to stay tuned to this latest episode of Peak Performance Selling. PEAK PERFORMANCE HIGHLIGHTS:KEVIN: MINDSET BALANCE BETWEEN BURNOUT AND RESILIENCE“I realized I needed to learn the other side of mindset, which is like how not to burn out and how to stay strong and be resilient.”KEVIN: YOUR EMOTION CREATES YOUR MINDSET“Emotion is what creates memory. Emotion is what creates new mindsets. If you want to get confident, have natural confidence, you got to get really excited about you being confident, and that will wire and fire and build new neural networks for confidence.”KEVIN: AVOID CREATING LIMITING BELIEFS“Last thing you want to do is get into an already bad situation and make it worse because you turn a bad situation into a limiting belief that lasts with you for the rest of your sales career.” You can connect with Kevin and check out his work in the links below:Kevin Bailey | Dreamfuel | Dreamfuel.com If you're listening to the Peak Performance Selling Podcast, please subscribe, share, and send us your feedback.Jordan Benjamin | MyCoreOs.com | Podcast | Email | Twitter

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2023.02.08.527340v1?rss=1 Authors: Torres-Rodriguez, J. M., Wilson, T. D., Singh, S., Chaudhry, S., Adke, A. P., Becker, J. J., Lin, J. L., Martinez Gonzalez, S., Soler-Cedeno, O., Carrasquillo, Y. Abstract: The spino-ponto-amygdaloid pathway is a major ascending circuit relaying nociceptive information from the spinal cord to the brain. Potentiation of excitatory synaptic transmission in the parabrachial nucleus (PbN) to central amygdala (CeA) pathway has been reported in rodent models of persistent pain. At the behavioral level, the PbN[->]CeA pathway has been proposed to serve as a general alarm system to potential threats that modulates pain-related escape behaviors, affective-motivational (but not somatosensory) responses to painful stimuli, aversion, and threat memory. Increased sensitivity to previously innocuous somatosensory stimulation is a hallmark of chronic pain. Whether the PbN[->]CeA circuit contributes to heightened sensitivity to somatosensory stimulation following an injury, however, remains unknown. Here, we demonstrate that activation of CeA-projecting PbN neurons contributes to injury-induced behavioral hypersensitivity but not baseline nociception in male and female mice. Using optogenetic assisted circuit mapping, we confirmed a functional excitatory projection from PbN[->]CeA that is independent of the genetic or firing identity of CeA cells. We then showed that peripheral noxious stimulation increases the expression of the neuronal activity marker c-Fos in CeA-projecting PbN neurons and chemogenetic inactivation of these cells reduces behavioral hypersensitivity in models of neuropathic and inflammatory pain without affecting baseline nociception. Lastly, we show that chemogenetic activation of CeA-projecting PbN neurons is sufficient to induce bilateral hypersensitivity without injury. Together, our results demonstrate that the PbN[->]CeA pathway is a key modulator pain-related behaviors that can amplify responses to somatosensory stimulation in pathological states without affecting nociception under normal physiological conditions. Copy rights belong to original authors. Visit the link for more info Podcast created by Paper Player, LLC

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2023.01.27.525952v1?rss=1 Authors: Gupta, R., O'Donnell, C. Abstract: Dendritic spines host glutamatergic excitatory synapses and compartmentalize biochemical signalling underlying synaptic plasticity. The narrow spine neck that connects the spine head with its parent dendrite is the crucial structural element of this compartmentalization. Both neck morphology and its molecular composition differentially regulate exchange of molecular signals between the spine and rest of the neuron. Although these spine neck properties themselves show activity-dependent plasticity, it remains unclear what functional role spine neck plasticity plays in synaptic plasticity expression. To address this, we built a data-constrained biophysical computational model of AMPA receptor (AMPAR) trafficking and intracellular signalling involving Ca2+calmodulin-dependent kinase II (CaMKII) and the phosphatase calcineurin in hippocampal CA1 neurons, which provides new mechanistic insights into spatiotemporal AMPAR dynamics during long-term potentiation (LTP). Using the model, we tested how plasticity of neck morphology and of neck septin7 barrier, which specifically restricts membrane protein diffusion, affect LTP. We found that spine neck properties control LTP by regulating the balance between AMPAR and calcineurin escape from the spine. Neck plasticity that increases spine-dendrite coupling reduces LTP by allowing more AMPA receptors to diffuse away from the synapse. Surprisingly, neck plasticity that decreases spine-dendrite coupling can also reduce LTP by trapping calcineurin, which dephosphorylates AMPARs. Further simulations showed that the precise timescale of neck plasticity, relative to AMPAR and enzyme diffusion and phosphorylation dynamics, critically regulates LTP. These results suggest a new mechanistic and experimentally-testable theory for how spine neck plasticity regulates synaptic plasticity. Copy rights belong to original authors. Visit the link for more info Podcast created by Paper Player, LLC

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2022.12.01.518760v1?rss=1 Authors: Bridi, M., Hong, S. Z., Severin, D., kirkwood, a. Abstract: Monocular deprivation (MD) causes an initial decrease in synaptic responses to the deprived eye in juvenile mouse primary visual cortex (V1) through Hebbian long-term depression (LTD). This is followed by a homeostatic increase, which has been attributed to synaptic scaling. However, homeostasis during other forms of visual deprivation is caused by sliding the threshold for Hebbian long-term potentiation (LTP) rather than scaling. We therefore asked whether the homeostatic increase during MD requires GluN2B-containing NMDA receptor activity, which is required to slide the plasticity threshold but not for synaptic scaling. Selective GluN2B blockade from 2-6d after monocular lid suture prevented the homeostatic increase in miniature excitatory postsynaptic current (mEPSC) amplitude in monocular V1 of acute slices and prevented the increase in visually evoked responses in binocular V1 in vivo. The decrease in mEPSC amplitude and visually evoked responses during the first 2d of MD also required GluN2B activity. Together, these results indicate that GluN2B-containing NMDA receptors first play a role in LTD immediately following eye closure, and then promote homeostasis during prolonged MD by sliding the plasticity threshold in favor of LTP. Copy rights belong to original authors. Visit the link for more info Podcast created by Paper Player, LLC

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2022.11.16.516747v1?rss=1 Authors: SAYEGH, F. J., Macri, C., Pi Macedo, J., Lejards, C., Rampon, C., Verret, L., DAHAN, L. S. Abstract: In most models, dopamine is not involved in the initiation but only in the long-term maintenance of LTP and memory. Conversely, we discovered that repeatedly stimulating Schaffer collaterals in concomitance with midbrain dopamine afferents to CA1 triggers hippocampal LTP. Moreover, we show that this dopamine pathway is involved in contextual learning. Thus, midbrain dopamine can play the role of a teaching signal triggering non Hebbian LTP and allowing supervised learning. Copy rights belong to original authors. Visit the link for more info Podcast created by Paper Player, LLC

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2022.11.16.516613v1?rss=1 Authors: Jusyte, M., Blaum, N., Boehme, M. A., Berns, M. M., Bonard, A. E., Kobbersmed, J. R., Walter, A. M. Abstract: Chemical synaptic transmission relies on neurotransmitter release from presynaptic release sites and on transmitter-sensing by the postsynaptic cell. Presynaptic plasticity increasing neurotransmitter release achieves two fundamental nervous system functions: It tunes some synapses to be more responsive to millisecond repetitive activation and it maintains signals when postsynaptic transmitter sensitivity is reduced. How enhanced neurotransmitter release is achieved in these phenomena, termed short-term facilitation and homeostatic potentiation, remains unknown. We combine mathematical modeling and experimental analysis of Drosophila neuromuscular junction model synapses to elucidate the molecular mechanisms underlying these forms of plasticity. Our results indicate that both phenomena depend on a rapid increase in the participation of neurotransmitter release sites which is controlled by the regulatory domains of the evolutionarily conserved (M)Unc13A protein that bind Ca2+/Calmodulin and diacylglycerol. Mutation of the Calmodulin binding (CaM) domain increased baseline transmission and impaired both short-term facilitation and acute homeostatic potentiation. Mathematical modeling indicated that these defects result from too many release sites participating at rest combined with the inability to plastically further increase their number. Super-resolution microscopy revealed that this coincided with a redistribution of Unc13A`s functionally essential MUN domain closer to the synaptic plasma membrane, which may constitute the molecular switch to increase release site participation. Similar consequences (enhanced baseline transmission, block of both short-term facilitation and homeostatic potentiation) were caused by the acute pharmacological activation of the C1 domain of wildtype Unc13A using phorbol esters. This treatment had no effect on Unc13A CaM domain mutants, indicating that both the CaM and C1 domains activate a binary release site switch. Thus, our findings indicate that Unc13A regulatory domains are tuned to integrate a multitude of signals on various timescales to switch release site participation for synaptic plasticity. Copy rights belong to original authors. Visit the link for more info Podcast created by Paper Player, LLC

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2022.10.28.514202v1?rss=1 Authors: Koppensteiner, P., Bhandari, P., Önal, C., Borges-Merjane, C., Le Monnier, E., Nakamura, Y., Sadakata, T., Sanbo, M., Hirabayashi, M., Brose, N., Jonas, P., Shigemoto, R. Abstract: GABAB receptor (GBR) activation inhibits neurotransmitter release in axon terminals in the brain, except in medial habenula (MHb) terminals, which show robust potentiation. However, mechanisms underlying this enigmatic potentiation remain elusive. Here, we report that GBR activation induces a transition from tonic to phasic release accompanied by a 4-fold increase in readily releasable pool (RRP) size in MHb terminals, mirrored by a similar increase in the docked vesicle number at the presynaptic active zone (AZ). The tonic and phasic release vesicles have distinct coupling distances. We identified two vesicle-associated molecules, synaptoporin and CAPS2, selectively involved in tonic and phasic release, respectively. Synaptoporin mediates augmentation of tonic release and CAPS2 stabilizes readily releasable vesicles during phasic release. A newly developed Flash and Freeze-fracture method revealed selective recruitment of CAPS2 to the AZ during phasic release. Thus, we propose a novel two-pool mechanism underlying the GBR-mediated potentiation of release from MHb terminals. Copy rights belong to original authors. Visit the link for more info Podcast created by Paper Player, LLC

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2022.08.31.506141v1?rss=1 Authors: Dannhäuser, S., Mrestani, A., Gundelach, F., Pauli, M., Komma, F., Kollmannsberger, P., Sauer, M., Heckmann, M., Paul, M. Abstract: Neurotransmitter release at presynaptic active zones (AZs) requires concerted protein interactions within a dense 3D nano-hemisphere. Among the complex protein mesh- work the (M)unc-13 family member Unc-13 of Drosophila melanogaster is essential for docking of synaptic vesicles and transmitter release. We employ MiMIC-based gene editing using GFSTF (EGFP-FlAsH-StrepII-TEV- 3xFlag) to endogenously tag all annotated Drosophila Unc-13 isoforms enabling visualization of endogenous Unc-13 expression within the central and peripheral nervous system. Electrophysiological characterization using two-electrode voltage clamp (TEVC) reveals that evoked and spontaneous synaptic transmission remain unaffected in unc-13 GFSTF 3 rd instar larvae and acute presynaptic homeostatic potentiation (PHP) can be induced at control levels. Furthermore, multi-color structured-illumination shows precise co-localization of Unc-13 GFSTF , Bruchpilot and GluRIIA-receptor subunits within the synaptic mesoscale. Localization microscopy in combination with HDBSCAN algorithms detect Unc-13 GFSTF nanoclusters that move towards the AZ center during PHP with unaltered Unc-13 GFSTF protein levels. Copy rights belong to original authors. Visit the link for more info Podcast created by PaperPlayer

This was a behemoth of an episode. We were joined by Mike Tuchscherer, a man who has essentially created his own training system, Emerging Strategies. During the show, we deep dive on peaking and athlete response, sequencing training blocks, and why Mike hasn't developed an Emerging Strategies AI. Check out our seminars in Calgary on Sept. 10th and 11th:Powerlifting: https://maxsgym.pike13.com/pack_products/795776Weightlifting: https://maxsgym.pike13.com/pack_products/795775SuperTotal: https://maxsgym.pike13.com/pack_products/795779Check out Mike's work:https://www.instagram.com/miketuchscherer/https://www.reactivetrainingsystems.com/Home/MainCheck out Max's work:https://www.instagram.com/max_aita/https://www.teamaita.com/Check me out for 1:1 Remote Coaching:https://www.instagram.com/josh_philwl/https://www.philosophicalweightlifting.com/Sign up for the best programming available: https://weightlifting.ai/Onyx:https://www.onyxstraps.com/ with code PHILWL for 10% offhttps://www.instagram.com/onyx_straps/Weightlifting House:https://www.weightliftinghouse.com/ with code PHILWL for 10% off

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Bringing back the man, they myth, the legend, Chad Trudo - The Direction of Strength & Conditioning at Aurora University. In this episode, Chad and I break down a recent article on post-activation potentiation. The theory behind this tactic is the use of a traditional exercise followed by a high velocity movement with hopes that the traditional exercise primed the nervous system to perform better during the high velocity movement. Kind of a mouthful there, but the idea has some real prowess in the world of strength and conditioning with tons of data supporting its use. But, before you apply this strategy there are a few things you may need to consider, such as: 1. How heavy of a load should I use during the strength exercise, and for how many reps? 2. What kind of high velocity movement pairs well? 3. How long of a rest interval should I use between the two movements? 4. How can I make sure fatigue doesn't affect my performance? These are just a few questions we attempt to answer using the latest data. Dive in and get all the juicy details.Guest: Chad TrudoIG: @coachtrudoArticle:Tsoukos, A., et. al. Potentiation of bench press throw performance using a heavy load and velocity-based repetition control. Journal of Strength and Conditioning Research 35(2S)/S72-S79.

Ep. 45 NEW SWAG! Training Elderly individuals, How your MIND can change your body, Post Activation Potentiation --- This episode is sponsored by · Anchor: The easiest way to make a podcast. https://anchor.fm/app Support this podcast: https://anchor.fm/the-daily-dose6/support

Baby grey seals. Polar bears. Zooplankton on painkillers. How do toxic chemicals and substances end up in Arctic animals — and as it happens, native people, too? Our guests on today's show are Bjørn Munro Jenssen, an ecotoxicologist at NTNU, Jon Øyvind Odland, a professor of global health at NTNU and a professor of international health at UiT —The Arctic University of Norway, and Ida Beathe Øverjordet, a researcher at SINTEF.One of the most useful websites on arctic pollution is the Arctic Monitoring and Assessment Programme, AMAP. Rachel Carson's book is Silent Spring.Here's a selection of articles from today's episode:Sørmo, E.G., Salmer, M.P., Jenssen, B.M., Hop, H., Bæk, K., Kovacs, K.M., Lydersen, C., Falk-Petersen, S., Gabrielsen, G.W., Lie, E. and Skaare, J.U. (2006), Biomagnification of polybrominated diphenyl ether and hexabromocyclododecane flame retardants in the polar bear food chain in Svalbard, Norway. Environmental Toxicology and Chemistry, 25: 2502-2511. https://doi.org/10.1897/05-591RBourgeon, Sophie; Riemer, Astrid Kolind; Tartu, Sabrina; Aars, Jon; Polder, Anuschka; Jenssen, Bjørn Munro; Routti, Heli Anna Irmeli. (2017) Potentiation of ecological factors on the disruption of thyroid hormones by organo-halogenated contaminants in female polar bears (Ursus maritimus) from the Barents Sea. Environmental Research. vol. 15Nuijten, RJM; Hendriks, AJ; Jenssen, Bjørn Munro; Schipper, AM. (2016) Circumpolar contaminant concentrations in polar bears (Ursus maritimus) and potential population-level effects. Environmental Research. vol. 151.Chashchin, Valery; Kovshov, Aleksandr A.; Thomassen, Yngvar; Sorokina, Tatiana; Gorbanev, Sergey A.; Morgunov, Boris; Gudkov, Andrey B.; Chashchin, Maxim; Sturlis, Natalia V.; Trofimova, Anna; Odland, Jon Øyvind; Nieboer, Evert. (2020) Health risk modifiers of exposure to persistent pollutants among indigenous peoples of Chukotka. International Journal of Environmental Research and Public Health (IJERPH). vol. 17 (1). See acast.com/privacy for privacy and opt-out information.

Contributor: Chris Holmes, MD Educational Pearls: Many are taught that patients with cocaine chest pain should not receive beta-blockers due to unopposed alpha agonism, but is this true? 363 consecutive admissions for chest pain with positive cocaine on urine toxicology were reviewed in a retrospective cohort study 60 patients in this cohort received a beta-blocker and multivariate analysis demonstrated a reduction in myocardial infarction risk Another retrospective cohort study demonstrated no association of negative outcomes with beta-blocker administration in those with a recent positive result on cocaine urine toxicology Two more recent meta-analyses were performed finding no association between adverse clinical outcomes and beta-blocker administration for cocaine chest pain No prospective randomized-controlled trials have been performed to evaluate the use of beta-blockers for treatment of cocaine chest pain in the ED setting References Dattilo PB, Hailpern SM, Fearon K, Sohal D, Nordin C. Beta-blockers are associated with reduced risk of myocardial infarction after cocaine use [published correction appears in Ann Emerg Med. 2008 Jul;52(1):90]. Ann Emerg Med. 2008;51(2):117-125. doi:10.1016/j.annemergmed.2007.04.015 Rangel C, Shu RG, Lazar LD, Vittinghoff E, Hsue PY, Marcus GM. Beta-blockers for chest pain associated with recent cocaine use. Arch Intern Med. 2010;170(10):874-879. doi:10.1001/archinternmed.2010.115 Pham D, Addison D, Kayani W, et al. Outcomes of beta blocker use in cocaine-associated chest pain: a meta-analysis. Emerg Med J. 2018;35(9):559-563. doi:10.1136/emermed-2017-207065 Lo KB, Virk HUH, Lakhter V, et al. Clinical Outcomes After Treatment of Cocaine-Induced Chest Pain with Beta-Blockers: A Systematic Review and Meta-Analysis. Am J Med. 2019;132(4):505-509. doi:10.1016/j.amjmed.2018.11.041 Richards JR, Hollander JE, Ramoska EA, et al. β-Blockers, Cocaine, and the Unopposed α-Stimulation Phenomenon. J Cardiovasc Pharmacol Ther. 2017;22(3):239-249. doi:10.1177/1074248416681644 Lange RA, Cigarroa RG, Flores ED, et al. Potentiation of cocaine-induced coronary vasoconstriction by beta-adrenergic blockade. Ann Intern Med. 1990;112(12):897-903. doi:10.7326/0003-4819-112-12-897 Summarized by John Spartz, MS4 | Edited by Erik Verzemnieks, MD   The Emergency Medical Minute is excited to announce that we are now offering AMA PRA Category 1 credits™ via online course modules. To access these and for more information, visit our website at https://emergencymedicalminute.org/cme-courses/ and create an account.  Donate to EMM today!

Welcome all to IS PHARMACOLOGY DIFFICULT Podcast! I am Dr Radhika Vijay.With a good gracious planning to wave 2021 a "CHAO", let us prepare well for new chores in n ew year 2022. In today's episode I will talk about Drug Synergism, its subheads, types, and I will also cover Drug Selectivity, specificity, Therapeutic efficacy, therapeutic Index, (T.I.) , Therapeutic range /Window. All will be clearly described with apt examples, hope you find this verbal talk beneficial!! For all the updates and latest episodes of my podcast, please visit www.ispharmacologydifficult.com where you can also sign up for a free monthly newsletter of mine. It actually contains lot of updates about the medical sciences, drug information and my podcast updates also. You can follow me on different social media handles like twitter, insta, facebook and linkedin. They all are with same name "IS PHARMACOLOGY DIFFICULT". If you are listening for the first time, do follow me here, whatever platform you are consuming this episode, stay tuned, do rate and review on ITunes, Apple podcasts, stay safe, stay happy, stay enlightened, Thank you!!You can access various links viahttps://linktr.ee/ispharmacologydifficult

HIGHLIGHTS02:05 Framing an entrepreneurial mindset and the power of visualization05:58 Preventing burnout and applying mindset lessons to sales09:49 Neuroscience lessons for teams: Potentiation, depotentiation, and equanimity13:56 Showing leaders the benefits of meditation and visualization 17:58 Keep performing at your best with just 15 minutes of mental fitness activities29:39 Maintaining your positive mindset in the face of failure31:00 Explaining the physiology of mindset and the results that follow38:47 For skeptics: Science backs it up and you must try it to feel the results46:39 Habit creation: Repetition helps create habits, but it can also only take 1 instance51:22 The ability to feel joy no matter what happens externally is success52:16 Connect with KevinQUOTES10:44 "You want to get confident, have natural confidence? You got to get really excited about you being confident and that will wire and fire and build new neural networks for confidence. Same thing with gratitude or any other mindset you want to practice."11:11 "There are networks in your mind, limiting beliefs, bad mindsets: I'm not good enough, our product's too expensive, our competitors are better than us, dad said don't talk about money, those kinds of beliefs that you actually need to lose. So that's called depotentiation."12:04 "Every time you trash talk yourself, you're creating new neural networks of shitty beliefs. So to not do that to yourself, that's called equanimity. That's what cold showers and stuff are trying to train you to do, just stay cool no matter what happened because the last thing you want to do is get into an already bad situation and make it worse."20:38 "For most people, all they need to do is set intention in the morning, meditate in the afternoon, and practice gratitude in the evening."31:03 "Your physiology controls your emotions and your feelings, your emotions and feelings control your thoughts, your thoughts control your actions, your actions control your results."You can learn more and follow Kevin on the following links below.LinkedIn - https://www.linkedin.com/in/kevinjamesbailey/Website - https://dreamfuelcoaching.com/If you're listening to the Peak Performance Selling Podcast, please subscribe, share, and send us your feedback.LinkedIn - https://www.linkedin.com/in/jordanbenjamin/Website - http://mycoreos.com/Podcast - https://www.mycoreos.com/podcastEmail - Jordan@MyCoreOS.comTwitter - https://twitter.com/jbenj09

Dr. Amandha Vollmer: "Over 10 years ago I found the work of Sol Luckman. It was a joy to interview him. I love synchronicity. After we recorded this podcast, the very next day, up popped one of my posts in Facebook memories from a decade ago about DNA and crop circles. I emailed it to him and he wrote back: 'Funny you sent that article. Here it is in 2010 in my ezine: http://www.phoenixregenetics.org/component/content/article/35-dna-monthly-archives/132-dna-monthly-vol-6-no-2#crop.' "I thought that was marvelous on so many levels." His brilliant T-shirts can be found here: https://sol-luckman.pixels.com/shop/tshirts His website: http://www.phoenixregenetics.org His art: https://sol-luckman.pixels.com His blog: https://snooze2awaken.com Oracle Report: http://www.oraclereport.com (the astrologer he mentioned) You can find me, ADV, at: https://yumnaturals.store https://yummy.doctor https://healingwithDMSO Thank you for all and any support, big changes are afoot! EEK! https://www.patreon.com/yumnaturals https://yumnaturals.store/product/general-fee-or-donation/ "The Human Bioenergy BlueprintThe science of muscle testing (Applied Kinesiology or simply kinesiology) employs muscle response tests to determine allergies, emotional blockages, and even the truth or falsehood of given statements.For the purposes of this introduction, it is simply necessary to emphasize that muscle testing, properly utilized, can be a powerful tool for gathering and evaluating information that has been validated in numerous studies.My partner Leigh assisted me throughout the development of Potentiation and the Regenetics Method. We performed literally tens of thousands of tests with clients who had come to us suffering from various chronic ailments.At the time, over eight years ago, we were offering a form of energetic allergy elimination derived from Nambudripad's Allergy Elimination Technique and an offshoot of NAET called BioSET.One of our most important realizations about traditional allergy clearings was that these techniques employ a typically “Western” focus on the physical—even though the techniques themselves use pure energy!" Read more: http://www.phoenixregenetics.org/activations

Episode 22: Joe Jeffrey- Post Show Phase Recovery and Offseason Potentiation   In this episode of J3 University, Luke and I are joined by Joe Jeffrey to discuss the transition from contest prep into the offseason. The post show period is vulnerable time for rapid fat gain and in the presence of high levels of fatigue. The approach discusses will cover how to set up diet, training, cardio, health markers and PEDs from prep into post show and what that progression of all variable should be as we phase into the offseason.   Joe Jeffrey is an online physique coach with physique collective specializing in physique enhancement.   For more information on Joe Jeffrey:   Instagram: @Joe_physiquecollective   Website: www.physiquecollective.com     Timestamps   Joe Jeffrey Intro- 00:00:25   First week post show set up- 00:05:06   Post show body fat/weight re-gain - 00:11:07   Prep Duration- 00:23:50   Training/Programming moving in to Offseason- 00:27:38   PED changes post show- 00:32:09   Changes to T3 post show- 00:37:49   Changes to Clen post show- 00:40:63   Changes to Growth Hormone/Insulin- 00:42:17   Lab work post show- 00:51:12   Wrap Up- 01:05:59     J3 UNIVERSITY: http://j3university.com COACHING: http://J3sportsrd.com FACEBOOK: https://www.facebook.com/J3-University-103894631667528 INSTAGRAM: https://www.instagram.com/johnjewett3/   Luke Miller INSTAGRAM: https://www.instagram.com/noswitchfitness/?hl=en Luke Miller coaching: https://noswitchfitness.com/contact/  

Heal & Transform Your Life with Bestselling Author & Renowned Sound Healer Sol Luckman Enjoy this interview with acclaimed author and well-known sound healer Sol Luckman on "Out of the Bag" internet radio show. Topics in this lively discussion focused on the "revolutionary healing science" (NEXUS Magazine) of the Regenetics Method include vaccine injury and how to heal from it, how to fast-track your personal growth and spiritual development, and many more. For more information on the Regenetics Method, visit ... http://www.phoenixregenetics.org/ Download free sample chapters of the international bestselling POTENTIATE YOUR DNA ... http://www.phoenixregenetics.org/books/potentiate-your-dna Register for our monthly free online Potentiation ceremony ... http://www.phoenixregenetics.org/resources/worldwide-potentiation-ceremony "Immunize" yourself against vaccine injury with the Regenetics Method ... https://snooze2awaken.com/2020/06/26/immunize-yourself-against-vaccine-injury-with-the-regenetics-method/ Evolve your life with DNA activation ... https://snooze2awaken.com/2020/09/15/evolving-your-life-with-dna-activation/ Visit Sol Luckman's personal website ... http://www.crowrising.com/ Check out his blog ... https://snooze2awaken.com/ Enjoy the original podcast of this interview complete with an hour of great music at ... http://outofthebagradio.weebly.com/podcasts/podcast-sean-maguire-sol-luckman-rebellion-hour-14102020 DISCLAIMER: The Developers and all Facilitators of the Regenetics Method offer DNA activation as educators and ordained ministers, not medical doctors, and do not purport to diagnose, prevent or treat illness of any kind. Regenetics Method information and sessions are offered, and accepted, as exercises of freedom of speech and religion. The Developers and Facilitators of the Regenetics Method make no recommendations, claims, promises or guarantees relative to specific health challenges. You are solely responsible for your own medical treatment and care.

Huge thanks to Pablo for coming on and sharing his awesome experience as an athlete and coach! Part 1 Toys for Kids in Honduras, we’re going!! We will be asking for your support down the road! Our first bikes! All things Pablo trains with: Road, MTB, Gym! PC Endurance Coaching Strength & Power, Skill Days, Power Based Training (MTB vs Road Bike) Tuesday MTB, Thurs Road Bike How Pablo lifts when there are no races (heavier weight)…higher reps and slower movements When races come, shorter workouts and shorter movements. Potentiation: works for higher end, but long efforts are very tough! Can we lift heavy on the day of long intervals? Not letting tertiary lifts go to the wayside. Sleep and Heart Rate Variability (HRV) Tracking non-bike TSS Endurance isn’t sexy. Going overkill on the Performance Manager Chart. The importance of consistency even when the volume can’t be high due to work and school. Riding after the rest week.

In this episode we update our understanding of the Pain Gate Theory and how synaptic behaviour can help reduce chronic pain. Understanding habituation, sensitization and adaptation is essential to long term recovery for clients experiencing chronic pain. Support the show (https://www.buymeacoffee.com/troylavigne)

Featuring an interview with Dr Zofia Piotrowska on the following topics: Current and emerging strategies for patients with non-small cell lung cancer (NSCLC) harboring an EGFR exon 20 insertion mutation (0:00) Case: A woman in her mid-50s with metastatic NSCLC and an EGFR exon 20 insertion receives mobocertinib as second-line therapy on a clinical trial (16:40) Case: A man in his late 60s with advanced NSCLC and an EGFR exon 20 insertion receives amivantamab as second-line therapy (21:11) Activity of mobocertinib in patients with advanced NSCLC and EGFR exon 20 insertions in the first-line setting; mechanism of action of amivantamab, a bispecific antibody targeting EGFR and MET (23:47) Cardiac toxicities associated with EGFR tyrosine kinase inhibitors (27:29) Efficacy of osimertinib in patients with NSCLC and EGFR exon 20 insertions (29:48) Therapeutic approach for patients with NSCLC and HER2 alterations (32:10) Role of immune checkpoint inhibitors for patients with NSCLC and targetable mutations (37:36) Management of NSCLC in patients with actionable alterations who develop brain metastases (41:33) Development of resistance in patients with NSCLC and targetable alterations; use of liquid biopsies for monitoring patients (44:37) EGFR-mutated adenocarcinomas that transform to small-cell lung cancer and other neuroendocrine carcinomas (47:18) Adjuvant therapy for patients with NSCLC and targetable mutations (51:01) Therapeutic approach for patients with locally advanced unresectable NSCLC and EGFR mutations (56:25) Potentiation of the risk of immune-related adverse events in patients receiving immunotherapy followed by osimertinib (59:00) Perspective on COVID-19 vaccination for patients with lung cancer; experience caring for patients with COVID-19 (1:04:11) CME information and select publications

This week we discuss Post-Activation Potentiation (PAP): the upregulation of muscle contractile ability as an affect of previous muscular contractions. This is a juicy one. Join us as we discuss what PAP is, and how it can be utilized for exercise.

Space Pyramyds and Jim belushi --- This episode is sponsored by · Anchor: The easiest way to make a podcast. https://anchor.fm/app

In this week's Doxit Podcast we discuss the January 2021 update to the Peaceful Pill eHandbook which focuses on the two issues of premedication and potentiation and the role both play in a peaceful and reliable death. Potentiation of lethal drugs is relevant if one has old drugs or substances or if they are from dubious sources or if they have been stored for a long time. Potentiators are also the drugs that bring about sedation, ensuring a more acceptable ‘sleep while you die' process.

Retrouvez dans cet épisode des SuperPhysique Podcast de Musculation, Rudy Coia et Fabrice, les fondateurs du site SuperPhysique.org destiné aux pratiquants de musculation sans dopage répondre aux meilleures questions de la semaine des Forums SuperPhysique. Cette semaine, on parle de lumbago, de recommandations diététiques, de potentiation, d'astuces, de séries télé, de presse à cuisse verticales....Le tout bien évidemment saupoudré d'anecdotes de vieux en tout genre pour votre plus grand plaisir. Si vous souhaitez poser vos questions, cela se passe directement sur https://www.superphysique.org Les liens du Podcast : - La FORMATION GRATUITE SuperPhysique - https://bit.ly/2UnAYye - Le LIVRE de Fabrice - https://bit.ly/2WOVV6O - L'application SP-TRAINING - https://bit.ly/3dI9RWq - Pour se faire COACHER par Rudy - https://www.rudycoia.com - Notre marque de COMPLÉMENTS alimentaires - https://bit.ly/3bsJIc8

Check out the full EVOQ blog with tons of free training tips: www.evoq.bike/blog If this content helps you out, please LIKE the video and SUBSCRIBE! We need your help! We charge you $0; the only currency we are asking for is SOCIAL SHARES. I got to catch up with Ashton who is training at altitude in Colorado right now for a couple of blocks. We touch on a TON of awesome topics, including: The science of lifting on the DAY OF A CYCLING EVENT! The term Potentiation came up, and he achieves this with Rack Pulls in the morning. What is he doing with altitude training in Colorado right now? What is a Brevet or Randonneuring? One big thing that he has found valuable in cycling training is Focusing on Time in Zone, not just average power for the duration…I love this. Don’t obsess about the drop in power from 20 seconds of coasting and then SLAM on the gas to bump the watts up more. What wins a bike race? Fastest Average Speed! What is he training for as we speak in November, since there is limited track access? Maybe the Hour Record! Ashton is now coaching with Strength Institute, we chat on this new chapter! ***Ashton dropped a couple F bombs that i didn't edit out, so please take notice if listening with kids.*** Also, what does Bahama Long Bottom mean? (Don't urban dictionary this term.) Brendan@EVOQ.BIKE Please share this! https://youtu.be/mM8exgBoZGw

If you enjoy this please share! Feel free to reach out if you have questions or would like to contribute to this podcast. Chris@BlueWaveFitness.com --- Support this podcast: https://anchor.fm/chris-lofland/support

Post Activation Potentiation... Any use to you?? If you would like to find more content like this you can go look at our Instagram and Youtube Accounts: https://www.youtube.com/channel/UCoFUI8OrYvZJcqfjo_bbl8Q https://www.instagram.com/sikastrength As always, if you enjoy the content and would like to support us, you can find us on Patreon: http://patreon.com/sikastrengthpodcast

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2020.10.27.358275v1?rss=1 Authors: Benoy, A., Bin Ibrahim, M. Z., Behnisch, T., Sreedharan, S. Abstract: Hippocampal CA2, an inconspicuously positioned area between the well-studied CA1 and CA3 subfields, has captured research interest in recent years due to its role in the formation of social memory. The effects of synaptic depression for subsequent long-term potentiation (LTP) of synaptic transmission at entorhinal cortical (EC)-CA2 and Schaffer collateral (SC)-CA2 synapses have not been previously explored. Here we show that cholinergic receptor activation with the non-selective cholinergic agonist carbachol (CCh) triggers a long-term synaptic depression (CCh-LTD) of field excitatory postsynaptic potentials at EC- and SC-CA2 synapses in the hippocampus of adult rats. The activation of muscarinic acetylcholine receptors (mAChRs) is critical for the induction of an early phase (100 min) of CCh-LTD. Importantly, CCh priming lowers the threshold, in a protein synthesis-dependent manner, for the late maintenance of LTP that can be subsequently induced by high-frequency electrical stimulation at EC- or SC-CA2 pathways. The results demonstrate that CA2 synaptic learning rules are modified in a metaplastic manner, wherein synaptic modifications triggered by cholinergic stimulation can dictate the outcome of future plasticity events. Moreover, the observed enabling of late LTP at EC inputs to CA2 following the priming stimulus co-exists with concurrent sustained CCh-LTD at SC-CA2 and is dynamically scaled by modulation of SC-CA2 synaptic transmission. Copy rights belong to original authors. Visit the link for more info

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2020.10.23.352377v1?rss=1 Authors: Makani, S., Lutzu, S., Lituma, P. J., Hunt, D. L., Castillo, P. E. Abstract: In the hippocampus, the excitatory synapse between dentate granule cell axons - or mossy fibers (MF) - and CA3 pyramidal cells (MF-CA3) expresses robust forms of short-term plasticity, such as frequency facilitation and post-tetanic potentiation (PTP). These forms of plasticity are due to increases in neurotransmitter release, and can be engaged when dentate granule cells fire in bursts (e.g. during exploratory behaviors) and bring CA3 pyramidal neurons above threshold. While frequency facilitation at this synapse is limited by endogenous activation of presynaptic metabotropic glutamate receptors, whether MF-PTP can be regulated in an activity-dependent manner is unknown. Here, using physiologically relevant patterns of mossy fiber stimulation in acute mouse hippocampal slices, we found that disrupting postsynaptic Ca2+ dynamics increases MF-PTP, strongly suggesting a form of Ca2+-dependent retrograde suppression of this form of plasticity. PTP suppression requires a few seconds of MF bursting activity and Ca2+ release from internal stores. Our findings raise the possibility that the powerful MF-CA3 synapse can negatively regulate its own strength not only during PTP-inducing activity typical of normal exploratory behaviors, but also during epileptic activity. Copy rights belong to original authors. Visit the link for more info

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2020.10.12.336883v1?rss=1 Authors: Yeates, C. J., Frank, C. A. Abstract: Synapses and circuits rely on homeostatic forms of regulation in order to transmit meaningful information. The Drosophila melanogaster neuromuscular junction (NMJ) is a well-studied synapse that shows robust homeostatic control of function. Most prior studies of homeostatic plasticity at the NMJ have centered on presynaptic homeostatic potentiation (PHP). PHP happens when postsynaptic muscle neurotransmitter receptors are impaired, triggering retrograde signaling that causes an increase in presynaptic neurotransmitter release. As a result, normal levels of evoked excitation are maintained. The counterpart to PHP at the NMJ is presynaptic homeostatic depression (PHD). Overexpression of the Drosophila vesicular glutamate transporter (VGlut) causes an increase in the amplitude of spontaneous events. PHD happens when the synapse responds to the challenge by decreasing quantal content during evoked neurotransmission, again, resulting in normal levels of postsynaptic excitation. We hypothesized that there may exist a class of molecules that affects both PHP and PHD. Impairment of any such molecule could hurt the ability of a synapse to respond to any significant homeostatic challenge. We conducted an electrophysiology-based screen for blocks of PHD. While we did not observe a block of PHD in the genetic conditions screened, we instead found loss-of-function conditions that led to excess depression, i.e., a substantial deficit in evoked amplitude when combined with VGlut overexpression. The conditions causing this phenotype included a double heterozygous loss-of-function condition for genes encoding the inositol trisphosphate receptor (IP3R, itpr) and ryanodine receptor (RyR). IP3Rs and RyRs gate calcium release from intracellular stores. Pharmacological agents targeting IP3R and RyR recapitulated the genetic losses of these factors, as did lowering calcium levels from other sources. Our data are consistent with the idea that some factors required for homeostatic potentiation are also required for the synapse to achieve appropriate levels of homeostatic depression. Loss of such factors may disorient compensatory plasticity signals. Copy rights belong to original authors. Visit the link for more info

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2020.09.15.298513v1?rss=1 Authors: Ramani, M., Miller, K., Ambalavanan, N., McMahon, L. Abstract: Preterm infants exposed to supraphysiological oxygen (hyperoxia) during the neonatal period have hippocampal atrophy and cognitive dysfunction later in childhood and as adolescents. Previously, we reported that 14-week-old adult mice exposed to hyperoxia as newborns had spatial memory deficits and hippocampal shrinkage, findings that mirror those of adolescents who were born preterm. Area CA1 region of the hippocampus that is crucial for spatial learning and memory is highly vulnerable to oxidative stress. In this study, we investigated the long-term impact of neonatal hyperoxia exposure on hippocampal CA3-CA1 synaptic function. Male and female C57BL/6J mouse pups were continuously exposed to either 85% normobaric oxygen or air between postnatal days 2-14. Hippocampal slice electrophysiology at CA3-CA1 synapses was then performed at 14 weeks of age. We observed that hyperoxia exposed mice have heightened strength of basal synaptic transmission measured in input-output curves, increased fiber volley amplitude indicating increased axonal excitability, and heightened LTP magnitude at CA3-CA1 synapses, likely a consequence of increased postsynaptic depolarization during the tetanus. These data demonstrate that supraphysiological oxygen exposure during the critical neonatal developmental period leads to pathologically heightened CA3-CA1 synaptic function during early adulthood which may contribute to hippocampal shrinkage and learning and memory deficits we previously reported. Furthermore, these changes may account for cognitive disorders in children born preterm who were exposed to prolonged oxygen supplementation. Copy rights belong to original authors. Visit the link for more info

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2020.08.21.260638v1?rss=1 Authors: Orlando, M., Dvorzhak, A., Bruentgens, F., Maglione, M., Rost, B. R., Sigrist, S. J., Breustedt, J. M., Schmitz, D. Abstract: Synaptic plasticity is a cellular model for learning and memory. However, the expression mechanisms underlying presynaptic forms of plasticity are not well understood. Here, we investigate functional and structural correlates of long-term potentiation at large hippocampal mossy fiber boutons induced by the adenylyl cyclase activator forskolin. We performed two-photon imaging of the genetically encoded glutamate sensor iGlu u that revealed an increase in the surface area used for glutamate release at potentiated terminals. Moreover, time-gated stimulated emission depletion microscopy revealed no change in the coupling distance between immunofluorescence signals from calcium channels and release sites. Finally, by high-pressure freezing and transmission electron microscopy analysis, we found a fast remodeling of synaptic ultrastructure at potentiated boutons : synaptic vesicles dispersed in the terminal and accumulated at the active zones, while active zone density and synaptic complexity increased. We suggest that these rapid and early structural rearrangements likely enable long-term increase in synaptic strength. Copy rights belong to original authors. Visit the link for more info

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2020.08.14.251322v1?rss=1 Authors: Huson, V., Meijer, M., Dekker, R., ter Veer, M., Ruiter, M., van Weering, J., Verhage, M., Cornelisse, L. N. Abstract: Previously, we showed that modulation of the energy barrier for synaptic vesicle fusion boosts release rates supralinearly (Schotten, 2015). Here we show that mouse hippocampal synapses employ this principle to trigger Ca2+-dependent vesicle release and post-tetanic potentiation (PTP). We assess energy barrier changes by fitting release kinetics in response to hypertonic sucrose. Mimicking activation of the C2A domain of the Ca2+-sensor Synaptotagmin-1 (Syt1), by adding a positive charge (Syt1D232N) or increasing its hydrophobicity (Syt14W), lowers the energy barrier. Removing Syt1 or impairing its release inhibitory function (Syt19Pro) increases spontaneous release without affecting the fusion barrier. Both phorbol esters and tetanic stimulation potentiate synaptic strength, and lower the energy barrier equally well in the presence and absence of Syt1. We propose a model where tetanic stimulation activates Syt1 dependent and independent mechanisms that lower the energy barrier independently in an additive manner to produce PTP by multiplication of release rates. Copy rights belong to original authors. Visit the link for more info

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2020.08.12.247726v1?rss=1 Authors: Becker, M. F. P., Tetzlaff, C. Abstract: The maintenance of synaptic changes resulting from long-term potentiation (LTP) is essential for brain functions. Different LTP phases have been associated with diverse molecular processes and pathways, and the molecular underpinnings of LTP on the time scales of a few minutes or of several hours are well established. However, the principles underlying the maintenance of the early phase of LTP (E-LTP) on the intermediate time scale of 1-6 hours remains elusive. We hypothesize that specific features of postsynaptic receptor trafficking would be responsible for sustaining synaptic changes during this LTP phase. We test this hypothesis by formalizing a biophysical model that integrates several experimentally-motivated mechanisms. The model captures a wide range of experimental findings and predicts that synaptic changes are preserved for hours when the receptor dynamics are shaped by the interplay of structural changes of the spine in conjunction with increased trafficking from recycling endosomes and the cooperative binding of receptors. Furthermore, our model provides several predictions to verify experimentally our findings. Copy rights belong to original authors. Visit the link for more info

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2020.07.30.228155v1?rss=1 Authors: Perez-Alvarez, A., Yin, S., Schulze, C., Hammer, J. A., Wagner, W., Oertner, T. G. Abstract: In hippocampal pyramidal cells, a small subset of dendritic spines contain endoplasmic reticulum (ER). In large spines, ER frequently forms a spine apparatus, while smaller spines contain just a single tubule of smooth ER. Here we show that the ER visits dendritic spines in a non-random manner, targeting spines during periods of high synaptic activity. When we blocked ER motility using a dominant negative approach against myosin V, spine synapses became stronger compared to controls. We were not able to further potentiate these maxed-out synapses, but LTD was readily induced by low-frequency stimulation. We conclude that the brief ER visits to active spines have the important function of preventing runaway potentiation of individual spine synapses, keeping most of them at an intermediate strength level from which both LTP and LTD are possible. Copy rights belong to original authors. Visit the link for more info

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2020.07.08.192369v1?rss=1 Authors: Paul G Donlin-Asp, Claudio Polisseni, Robin Klimek, Alexander Heckel, Erin M Schuman Abstract: Decades of work have demonstrated that mRNAs are localized and translated within neuronal dendrites and axons to provide proteins for remodeling and maintaining growth cones or synapses. It remains unknown, however, whether specific forms of plasticity differentially regulate the dynamics and translation of individual mRNA species. To address these issues, we targeted three individual synaptically-localized mRNAs, CamkIIa, Beta actin, Psd95, and used molecular beacons to track endogenous mRNA movements and reporters and Crispr-Cas9 gene editing to track their translation. We found widespread alterations in mRNA behavior during two forms of synaptic plasticity, long-term potentiation (LTP) and depression (LTD). Changes in mRNA dynamics following plasticity resulted in an enrichment of mRNA in the vicinity of dendritic spines. Both the reporters and tagging of endogenous proteins revealed the transcript-specific stimulation of protein synthesis following LTP or LTD. The plasticity-induced enrichment of mRNA near synapses could be uncoupled from its translational status. The enrichment of mRNA in the proximity of spines allows for localized signaling pathways to decode plasticity milieus and stimulate a specific translational profile, resulting in a customized remodeling of the synaptic proteome.Competing Interest StatementThe authors have declared no competing interest.View Full Text Copy rights belong to original authors. Visit the link for more info

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2020.06.06.128926v1?rss=1 Authors: Valstad, M., Roelfs, D., Slapo, N. B., Timpe, C. M. F., Rai, A., Matziorinis, A. M., Beck, D., Richard, G., Saether, L. S., Haatveit, B., Nordvik, J. E., Hatlestad-Hall, C., Einevoll, G. T., Mäki-Marttunen, T., Haram, M., Ueland, T., Lagerberg, T. V., Steen, N. E., Melle, I., Westlye, L. T., Jönsson, E. G., Andreassen, O. A., Moberget, T., Elvsashagen, T. Abstract: Background. Several lines of research suggest that impairments in long-term potentiation (LTP)-like synaptic plasticity might be a key pathophysiological mechanism in schizophrenia (SZ) and bipolar disorder type I (BDI) and II (BDII). Using modulations of visually evoked potentials (VEP) of the electroencephalogram, impaired LTP-like visual cortical plasticity has been implicated in patients with BDII, while there has been conflicting evidence in SZ, a lack of research in BDI, and mixed results regarding associations with symptom severity, mood states, and medication. Methods. We measured the VEP of patients with SZ spectrum disorders (n=31), BDI (n=34), BDII (n=33), and other BD spectrum disorders (n=2), and age-matched healthy control participants (n=200) before and after prolonged visual stimulation. Results. Compared to healthy controls, modulation of VEP component N1b, but not C1 or P1, was impaired both in patients within the SZ spectrum ({chi}2=35.1, p=3.1x10-9 and BD spectrum ({chi}2=7.0, p=8.2x10-3), including BDI ({chi}2=6.4, p=0.012), but not BDII ({chi}2=2.2, p=0.14). N1b modulation was also more severely impaired in SZ spectrum than BD spectrum patients ({chi}2=14.2, p=1.7x10-4). The reduction in N1b modulation was related to PANSS total scores ({chi}2=10.8, p=1.0x10-3), and nominally to number of psychotic episodes ({chi}2=4.9, p=0.027). Conclusions. These results suggest that LTP-like plasticity is impaired in SZ and BDI, but not BDII, and related to psychotic symptom severity. Adding to previous genetic, pharmacological, and anatomical evidence, these results implicate aberrant synaptic plasticity as a mechanism underlying SZ and BD. Copy rights belong to original authors. Visit the link for more info

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2020.06.02.128900v1?rss=1 Authors: Field, M., Thomas, P., Smart, T. Abstract: GABAA receptors (GABAARs) are pentameric ligand-gated ion channels distributed throughout the brain where they mediate synaptic and tonic inhibition. Following activation, these receptors undergo desensitization which involves entry into long-lived agonist-bound closed states. Although the kinetic effects of this state are recognised and its structural basis has been uncovered, the physiological impact of desensitization on inhibitory neurotransmission remains unknown. Here we describe an enduring new form of long-term potentiation at inhibitory synapses that elevates synaptic current amplitude for 24 hrs following desensitization of GABAARs in response to prolonged agonist exposure or allosteric modulation. Using receptor mutants and allosteric modulators we demonstrate that desensitization of GABAARs facilitates their phosphorylation by PKC, which increases the number of receptors at inhibitory synapses. These observations provide a new physiological relevance to the desensitized state of GABAARs, acting as a signal to regulate the efficacy of inhibitory synapses during prolonged periods of inhibitory neurotransmission. Copy rights belong to original authors. Visit the link for more info

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2020.06.01.128710v1?rss=1 Authors: Prieto, M. L., Firouzi, K., Khuri-Yakub, B. T., Madison, D. V., Maduke, M. Abstract: Ultrasound can modulate action potential firing in vivo and in vitro, but the mechanistic basis of this phenomenon is not well understood. To address this problem, we used patch-clamp recording to quantify the effects of focused, high-frequency (43 MHz) ultrasound on evoked action potential firing in CA1 pyramidal neurons in acute rodent hippocampal brain slices. We find that ultrasound can either inhibit or potentiate firing in a spike-frequency-dependent manner: at low (near-threshold) input currents and low firing frequencies, ultrasound inhibits firing, while at higher input currents and higher firing frequencies, ultrasound potentiates firing. The net result of these two competing effects is that ultrasound increases the threshold current for action potential firing, the slope of frequency-input curves, and the maximum firing frequency. In addition, ultrasound slightly hyperpolarizes the resting membrane potential, decreases action potential width, and increases the depth of the afterhyperpolarization. All of these results can be explained by the hypothesis that ultrasound activates a sustained potassium conductance. According to this hypothesis, increased outward potassium currents hyperpolarize the resting membrane potential and inhibit firing at near-threshold input currents, but potentiate firing in response to higher input currents by limiting inactivation of voltage-dependent sodium channels during the action potential. This latter effect is a consequence of faster action-potential repolarization, which limits inactivation of voltage-dependent sodium channels, and deeper (more negative) afterhyperpolarization, which increases the rate of recovery from inactivation. Based on these results we propose that ultrasound activates thermosensitive and mechanosensitive, voltage-insensitive two-pore-domain potassium (K2P) channels, through heating or mechanical effects of acoustic radiation force. Finite-element modelling of the effects of ultrasound on brain tissue suggests that the effects of ultrasound on firing frequency are caused by a small (less than 2{degrees}C) increase in temperature, with possible additional contributions from mechanical effects. Copy rights belong to original authors. Visit the link for more info

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2020.05.10.087114v1?rss=1 Authors: Zhang, C.-Q., Catron, M. A., Ding, L., Hanna, C. M., Gallagher, M. J., Macdondald, R. L., Zhou, C. Abstract: Idiopathic generalized epilepsy(IGE) patients have genetic causes and their seizure onset mechanisms particularly during sleep remain elusive. Here we proposed that sleep-like slow-wave oscillations(0.5 Hz SWOs) potentiated excitatory or inhibitory synaptic currents in layer V cortical pyramidal neurons from wild-type(wt) mouse ex vivo brain slices. In contrast, SWOs potentiated excitatory, not inhibitory, currents in cortical neurons from heterozygous(het) knock-in(KI) IGE mice(GABAA receptor lower case Greek gamma2 subunit Gabrg2Q390X mutation), creating an imbalance between evoked excitatory and inhibitory currents to effectively prompt neuronal action potentials. Similarly, more physiologically similar up/down-state(present during slow-wave sleep) induction in cortical neurons could potentiate excitatory synaptic currents within slices from wt/het Gabrg2Q390X KI mice. Consequently, SWOs or up/down-state induction in vivo (using optogenetic method) could trigger epileptic spike-wave discharges(SWDs) in het Gabrg2Q390X KI mice. To our knowledge, this is the first operative mechanism to explain why epileptic SWDs preferentially happen during non-REM sleep or quiet-wakefulness in human IGE patients. Copy rights belong to original authors. Visit the link for more info

O convidado deste episódio é o Daniel moedas, fisioterapeuta da seleção nacional de natação também ligado à área do Strength and Conditioning. No episódio de hoje vamos abordar a temática do PAP e perceber o que a ciência nos tem dito nos últimos anos. Se te queres manter atualizado ouve este excelente episódio que não te vais arrepender.

The Coronavirus and Dating: How to Love When You're in a State of Fear? As a psychologist and dating coach, I'm thinking about how this coronavirus scare is currently affecting dating and how that might evolve.  The question on many singles' minds are: Will this coronavirus scare get worse, or perhaps, will it soon be contained, improve and will a vaccine be created? Dating is already often a process fraught with anxiety about the unknown. It can be challenging not to know if you'll like this stranger or if they'll like you if you will hear from your dates again, if they have STDs or if you'll feel physically and emotionally safe with them.  As a dating coach, it can be part of your job to encourage people to put themselves out there, physically and emotionally. Now, psychologically you are adding an additional layer of fear for some singles, because they're worrying about whether dating may expose them to the Coronavirus.  As a single they have no context for their date, for their date's travels or health, and Coronavirus carriers can be asymptomatic. Conversely, these aforementioned fears of dating are often on par with the opposite fear that some singles have about not meeting someone and being alone during this pandemic. My intention for this article is to spread love and healing, not fear.  Sometimes making our fears conscious can help us to better normalize and metabolize them and make us feel less alone. Of course, there are also many singles that are still dating and living their lives normally. They have not let fear change their inner or outer view of dating. Some report taking a Zen approach, remarking that we will all probably get the Coronavirus but that hopefully, it won't be that bad or deadly. Some people are even using humor to lighten this weighty topic and are putting Coronavirus pickup lines on their dating profiles. So, the fears and reactions from singles are mixed. One recent survey said that 33 percent of singles are worried about dating now. On the flip side, one dating app survey reported that dating app signups are up 29 percent.  Some singles are aiming to have a ‘virtual relationship,' which could be seen as a psychic and physical compromise so that they don't feel alone but also aren't physically exposed. Fears can make people contract, take fewer risks and can negatively impact their decision-making. Also, fear can breed more fear through a process called Potentiation, where once you are primed for fear than even benign events can seem scary.  People can to go into Freeze, Fight or Flight response when afraid.  Freezing means they would just stop dating and then decide what to do next.  If you have a Fight response, you'd decide how to directly deal with the threat.  If you tend to Flee or use Flight, you would avoid and work around this threat, like maybe just talking to people online and not in person, or avoiding dating for a while. During this unknown time of the coronavirus, some singles are experiencing a drive to isolate and an opposing one to powerfully connect and bond. It may help to make these fears conscious on both sides of the equation: Some Increased Fears that Singles are having re Dating: Fear of Physical Intimacy- The media has advised, ‘social distance' and have said that this coronavirus can be passed through a distance of 6 feet, through a cough or bodily fluid.  Where does this leave the dating ritual of, ‘the goodnight kiss?' It gives a whole new meaning to contraceptives and people have been posting funny pictures of all the outfit getups that people are already wearing about town to protect themselves.  ‘So, how does one look their best, flirt and romantically connect during a date while maintaining safety and social connection?' Fear of Emotional Intimacy- Some singles fear to connect and get close to someone new when they imagine that person could get sick and die soon. Also, they report being wary of adding the stress of rejection to their plate when they are already feeling anxious.  They say they'd feel better dating at a time when they are feeling happier and more confident. Fear of Public Spaces- Since the media has advised, ‘social distance,' many singles fear going to group events for singles or otherwise.  This could include concerts, singles events, and crowded bars. This makes it harder to put yourself out there to find love. Fear of the Unknown & Getting Sick- Since there is no solution to getting this virus- no vaccine or antidote yet- some singles are saying that prevention is key. They fear and want to avoid getting sick and fear a lack of control. So, they focus on having a degree of social isolation because it's what they feel they can do right now. Fear that You'll Be a Debby Downer- Singles report that it's hard to date and to be sexy and positive when the Coronavirus is the ‘White Elephant' in the room. Fear can exacerbate existing anxiety and depression and it can feel unattractive to date while not feeling your best. Some Singles are Having an Increased Desire to Connect & Find Love: To Extend Cuffing Season & Quarantine with a Partner Indefinitely- ‘Cuffing season,' is a phenomenon where for a period of time in winter singles pair off so that they have someone to stay home and snuggle with.  Now as people may be quarantined, some singles are reporting an increased desire to have that ‘someone special,' to keep them company at home. To Have that Secure Base- The attachment literature shows that in times of fear, people look to attach and to have that secure base of comfort.  Having that secure base helps people reengage with the world in brave ways.  So, it makes sense that in times of fear like with this virus that the desire to have that primary love bond would increase. The Desire To Be Part of a Team- Dealing with the unknown and changes can feel confusing.  The media has reported new cases, quarantines, hospitalizations, some travel bans, some changes in people working from home, some lay-offs, stock market losses, and many canceled events thus far.  Some people have chosen to follow suggestions to get a month's worth of food and hand sanitizer, just in case they are quarantined.  Some singles are expressing a desire to have a partner to cope with all these changes and to help them put appropriate plans in place. To Refocus on What Matters Most- Sometimes when big scary events strike, it makes people stop and refocus on their heart's desire and on what matters most to them.  Maybe they've reflexively been focused on work or other pressing matters but they really wanted a life partner and have not focused on it.  Sometimes a scary situation makes them realign their priorities and focus on what matters most to them, like finding love. Physical Closeness & Cuddling Can Calm Fears- Research shows that cuddling can improve sleep, increase oxytocin (the bonding hormone), ease pain, reduce anxiety, boost your immune system, and elevate your mood and more. So, while dating felt confusing enough, you can see why some singles may feel torn about the best ways to proceed with dating now and in the near future. Since I'm a big proponent of love, I hope that people will continue to meet and create happy, lasting relationships. Whether there will be longer virtual relationships, a decline in sex or physical intimacy while dating and more daters taking a hiatus from singles events and dating in general, time will tell. To learn more from Dr. Sherman and to find out about dating and Relationship coaching, go to www.DrPauletteSherman.com  

In recent years there has been a reinvigorated and growing interest in the investigation and use of potentiation complexes in strength and conditioning. In this episode we chat with Dr. Hugh Lamont to discuss the possible upsides and downsides of potentiation, mechanisms of postactivation potentiation (PAP) and strategies to consider when using potentiation complexes. This is a must listen for practitioners interested in or already using potentiation strategies!

The TWiN team explain the basic principles of synaptic transmission, and review a classic paper demonstrating that recycling endosomes are important for modifying synaptic strength, which is involved in information storage and processing. Hosts: Ori Lieberman, Andres Bendesky,Jason Shepherd, and Vincent Racaniello Subscribe (free): iTunes, Google Podcasts, RSS, email Become a patron of TWiN! Links for this episode Bendesky lab Recycling Endosomes Supply AMPA Receptors for LTP (Science) AMPA receptor (Wikipedia) Image credit Letters read on TWiN #2 Intro music is by Ronald Jenkees. Send your neuroscience questions and comments to twin@microbe.tv

In this episode I break down post activation potentiation and how you can use it in your training. Enjoy!

This week Joe sits down and talks shop with JL Holdsworth. JL is a former world-class powerlifter and current owner of The Spot Athletics - a performance facility with 2 locations in Columbus, OH. JL is also the co-founder of the RPR (Reflexive Performance Reset) system. Highlights from this thought-provoking conversation include: JL's gym/business philosophy; How/Why your "physiology" dictates your thoughts, words & actions; What is RPR; JL's definition of "activation"; JL's theory on why Kevin Durant tore his Achilles tendon; Defining the "flow state"; The sympathetic & parasympathetic nervous system "debate" (survival vs performance); Potentiation vs neurological firing sequence...and much, Much MORE! For Show Notes & Timestamps goto www.IndustrialStrengthShow.com

This week Joe sits down and talks shop with JL Holdsworth. JL is a former world-class powerlifter and current owner of The Spot Athletics - a performance facility with 2 locations in Columbus, OH. JL is also the co-founder of the RPR (Reflexive Performance Reset) system. Highlights from this thought-provoking conversation include: JL's gym/business philosophy; How/Why your "physiology" dictates your thoughts, words & actions; What is RPR; JL's definition of "activation"; JL's theory on why Kevin Durant tore his Achilles tendon; Defining the "flow state"; The sympathetic & parasympathetic nervous system "debate" (survival vs performance); Potentiation vs neurological firing sequence...and much, Much MORE! For Show Notes & Timestamps goto www.IndustrialStrengthShow.com

Happy Monday! Today, the guys are in the gym talking about post activation potentiation, or PAP for short. They define this term and talk about the potential underlying mechanisms of what is actually happening within the body. The guys dig into the who/what/when/how/why for using this for yourself and your athletes. patients or clients. Hope you enjoy today's episode!Support the show (http://paypal.me/betterfasterpod)

Chidi Enyia is a Sprints, Hurdles, and Jumps Coach for ALTIS, a fitness company designed to provide an environment where elite track and field athletes can grow to become better at their chosen sport. They have state-of-the-art training facilities, a robust clientele, and professional and certified coaches. He has over 20 years experience training athletes […] The post Chidi Enyia on Building Explosive Speed, Strength and Power with Potentiation appeared first on Robertson Training Systems.

In this third Q&A instalment of the podcast we delve into the use of isometrics, potentiation techniques, the mechanics of quadricep and hamstring training, and Luke decides to talk about faecal transplants.

For our 100th episode, we countdown our Top 10 Favorite JuggLife Parodies of all-time. Check it out at the Juggernaut Training Systems YouTube:  https://youtu.be/zshGT18GKFg

In Episode #3 of Training Chats with Israel and Mladen, we discuss the the concept of  phase potentiation in periodization. That is, does sequencing periods that place greater emphasis on certain qualities (strength, speed, etc) in a particular order lead to better performance compared with different sequences and/or without a specific emphasis on a particular quality. This is an important topic as the core of periodization models lays within this concept. We also touched the problems with simplistic reasoning attempting to explain the positive effects commonly observed with resistance training.  

This episode we go over an indepth article on periodization for powerlifters by Dr. Mike Israetel which can be found at the Juggernaut Training Systems website here. We cannot say enough great things about this guide. Dave learned the difference between micro, meso, and macrocycles. Everett and Janine debate over phase potentiation and Janine reads excerpts from her copy of Periodization Training for Sports by Bompa and Carrera. There are a number of helpful visuals you won't get in the podcast so please do check the link out above. Enjoy the show!

It is a pleasure to share this interview with my long-term colleague Dr. Ralph Moss, former science writer and Assistant Director of Public Affairs at Memorial Sloan-Kettering Cancer Center. Dr. Moss authored or edited 12 books on cancer research and treatment, including The Cancer Industry, Questioning Chemotherapy, and Doctored Results. Writer of the award-winning PBS documentary “The Cancer War,” he currently creates The Moss Reports summarizing evidence-based treatments for 36 different cancer diagnoses. Listen to this podcast, and hear Dr. Moss' well-researched opinions on: The cancer breakthrough of the year The truth about Laetrile research at Sloan-Kettering The immune system and spontaneous regression of cancer When chemotherapy is and is not appropriate The success of Insulin Potentiation Therapy The most effective therapies currently in clinical use The value of immune checkpoint drugs like Keytruda and Opdivo The promise of cancer-killing viral therapy How far we have come in the last 40 years and why he is optimistic

From chat rooms to forums, the Internet allows us to communicate anonymously about anything under the sun – the extremely useful and the unfortunately useless. The nootropic stack CILTEP is one of those “extremely useful” gems from an Internet forum via Longecity.org. The creator of this underground nootropic stack comes on the show for an exclusive talk about CILTEP and how it works. CILTEP is  a natural stack designed to improve and maintain brain performance, especially memory, focus, and motivation. On this episode you’ll learn about how it was created and why you should supplement with it for optimal performance. Abelard Lindsay has been doing software development professionally for 20 years. He is currently in his late 30s and in his second year of earning a masters in computer science.  Abelard started investigating alternative medicine and the brain about 10 years ago and was motivated to apply and go back to graduate school in computer science after he began taking the nootropic stack CILTEP because, as he says: “…it was so much easier and enjoyable for me to learn new material… CILTEP made me want to study.”

From chat rooms to forums, the Internet allows us to communicate anonymously about anything under the sun – the extremely useful and the unfortunately useless. The nootropic stack CILTEP is one of those “extremely useful” gems from an Internet forum via Longecity.org. The creator of this underground nootropic stack comes on the show for an exclusive talk about CILTEP and how it works. CILTEP is  a natural stack designed to improve and maintain brain performance, especially memory, focus, and motivation. On this episode you’ll learn about how it was created and why you should supplement with it for optimal performance. Abelard Lindsay has been doing software development professionally for 20 years. He is currently in his late 30s and in his second year of earning a masters in computer science.  Abelard started investigating alternative medicine and the brain about 10 years ago and was motivated to apply and go back to graduate school in computer science after he began taking the nootropic stack CILTEP because, as he says: “…it was so much easier and enjoyable for me to learn new material… CILTEP made me want to study.”

กดฟัง WiTcast ตอนที่ 9.2 download ไฟล์ MP3 (คลิกขวา save link as) PODCAST / iTUNES สำหรับผู้ที่ใช้ iPhone, ipod ท่านสามารถใช้โปรแกรม iTunes สมัครเป็นสมาชิกรายการให้โหลดเองอัตโนมัติได้ โดยเข้า iTunes store แล้วเสริชหา witcast หรือ subscribe ผ่าน feed นี้โดยตรง http://feeds.feedburner.com/witcast ร่วมบริจาคเงินสนับสนุนรายการได้โดยโอนเข้าบัญชี : ธนาคารไทยพาณิชย์ สาขาบิ๊กซี สะพานควาย เลขบัญชี 0332935256 ชื่อ แทนไท ประเสริฐกุล หรือส่งผ่าน paypal มาที่ yeebud@gmail.com ------------------------------------------------------------- SHOW NOTE - รังนก (แทนไท) อันนี้ความเห็นที่ไม่ได้พูดในรายการนะครับ - โดยส่วนตัว ผมไม่กินรังนกเพียงเพราะรู้สึกเฉยๆ กับรสชาติ แต่ถ้าถามว่ามีทัศนคติอย่างไรกับ "ธุรกิจ" รังนก ผมขอบอกว่ามีทัศนคติในแง่ลบครับ เหตุผลเพราะมันเป็นตัวแทนของหลายสิ่งที่ผมไม่เห็นด้วย เช่น ความเชื่อโบราณงมงาย ประเภทว่าอะไรโบราณต้องดีไว้ก่อน เห็นมั้ยเค้ากินกันมาเป็นพันปีจะไม่ดีได้ไง... กับอีกอย่างคือ มันเป็นตัวแทนของการโฆษณาชวนเชื่อเว่อร์เกินจริงของธุรกิจอาหารเสริมทั้งหลาย คือรังนกอาจจะมีสารมีประโยชน์บ้าง แต่งานวิจัยยืนยันยังถือว่าห่างไกลจากความชัดเจน แถมต่อให้มีก็ยังน่าจะเป็นสารที่พบในอาหารอื่นๆ อยู่แล้ว ร่างกายได้รับเพียงพออยู่แล้ว แต่นี่ดันโฆษณาสร้างภาพซะอย่างกับเป็นยาวิเศษสุดยอด คุณค่าคัดสรรสำหรับคนที่คุณรัก โอย...ฟังแล้วอยากถอนขนหัวนม - ข้อมูลของตัวนกแอ่นกินรัง เชิญที่บล็อกนี้ครับ เขียนเล่าไว้เยอะมากโดยนักวิจัยผู้ศึกษาจริงไรจริง (อ.ศุภลักษณ์ วิรัชพินทุ) แถมเล่าแบบน่ารักด้วย - บทความวิชาการ Characterization of the edible bird's nest the “Caviar of the East” -1,2 -ให้ข้อมูลพื้นฐานเยอะมาก รวมทั้งเรื่องวิเคราะห์องค์ประกอบรังนกขาว vs. รังนก "เลือด" ซึ่งไม่มีจริง มีแต่รังนกสีแดงที่เกิดจากแร่ธาตุผนังถ้ำซึมเข้าผสมกับรัง ไม่ได้เกิดจากแม่นกโดนขโมยรังจนขากเป็นเลือดอย่างที่คนชอบพูดๆ กันนะฮ้า - สารเจือปนที่นิยมนำมาทำรังนกปลอม - ประเด็น รังนกแท้ 100% vs 1% และคุณค่าทางโภชนาการที่เทียบต้องกิน 30 ขวดถึงจะเท่าไข่ 1 ฟอง -1,2,3,4,5,6,7,8 - ดราม่ารังนกใน pantip - 1,2,3,4,5,6,7 [youtube http://www.youtube.com/watch?v=AF53ReXTcmU] [youtube http://www.youtube.com/watch?v=EqhE5Tw7iRM] [youtube http://www.youtube.com/watch?v=ibuMuDXGmT0] [youtube http://www.youtube.com/watch?v=t7bC86wX43A] - ตัวอย่างข่าวดราม่ารังนกแนวการเมืองท้องถิ่น-ขัดแย้งผลประโยชน์ -1,2,3,4 บทความจาก Nat Geo - ธุรกิจสีเทาของรังนก ติดตามเส้นทางเดินอันผาดโผนของรังนก จากเถื่อนถ้ำสู่ภัตตาคารหรู - ประเด็นสรรพคุณ หรืองานวิจัยเกี่ยวกับสารออกฤทธิ์ที่อาจจะมีประโยชน์ ผมรู้แค่งูๆ ปลาๆ ใครมีความเชี่ยวชาญเฉพาะด้าน ช่วยกันวิเคราะห์ได้นะครับ 1. ฤทธิ์กระตุ้นการแบ่งเซล (ผิวหนัง, เยื่อบุต่างๆ, หรือเซลภูมิคุ้มกันเช่นเม็ดเลือดขาว) - (Ng et al. 1986) Potentiation of mitogenic response by extracts of the swiftlet's (Collocalia) nest. สรุปสั้นๆ สารสกัดรังนกทำให้เซลเม็ดเลือดขาวตอบสนองต่อสารกระตุ้นการแบ่งเซลได้ดีขึ้น วิจารณ์/ ข้อกังขา ทดลองในหลอดแก้ว ใช้รังนกสกัด ไม่เหมือนที่เรากิน อ่านดีๆ จะเห็นคำว่า "potentiation" คือตัวรังนกเองไม่ได้ช่วยกระตุ้นโดยตรง แต่ช่วยให้เซลตอบสนองต่อสารกระตุ้นอย่างอื่นดีขึ้น สารที่ว่า (lectins) ได้จากพวกถั่วอีกที เทียบระหว่างเสริมรังนกกับไม่เสริมรังนก แต่ไม่ได้เทียบระหว่างเสริมรังนกกับเสริมอย่างอื่น effect เยอะน้อยขนาดไหนไม่แน่ใจ เพราะอ่านได้แต่ abstract   - (Kong et al. 1987) Evidence that epidermal growth factor is present in swiftlet's (Collocalia) nest สรุปสั้นๆ สามารถสกัดสารที่คล้าย Epidermal Growth Factor (EGF) จากรังนก แสดงให้ดูว่าสารนี้ช่วยเสริมการดูดซึม Thymidine (ตัว T ในรหัสพันธุกรรม ATCG) เข้าสู่เซล fibroblasts (เซลที่สร้างเส้นใยคอลลาเจนในเนื้อเยื่อประสานของผิวหนัง รวมทั้งเยื่อบุภายในต่างๆ) เทียบคุณสมบัติแล้วใกล้เคียงกับ EGF ที่สกัดได้จากหนู วิจารณ์/ ข้อกังขา ทดลองในหลอดแก้ว ใช้รังนกสกัด ไม่เหมือนที่เรากิน EGF ถ้ากระตุ้นการแบ่งเซลมากไปสามารถก่อมะเร็งได้เหมือนกัน effect เยอะน้อยขนาดไหนไม่แน่ใจ เพราะอ่านได้แต่ abstract EGF ในน้ำลายกับน้ำย่อยเราก็มีอยู่แล้ว + ในนมก็มีอยู่แล้ว   - (Kong et al. 1989) Potentiation of mito-genic response by extracts of the swiftlet's (Apus) nest collected from Huai-Ji สรุปสั้นๆ

Channel 1 of The (NEXT) Series

Channel 1 of The (NEXT) Series

Homeostasis is a key concept in biology. It enables ecosystems, organisms, organs and cells to adjust their operating range to values that ensure optimal performance. Homeostatic regulation of synaptic activity has been shown to play an important role during development. Here I investigated whether also mature neurons possess mechanisms to prevent the strengthening of input synapses once the limit of their ‘operating range’ has been reached. Using electrophysiological recordings in hippocampal slices, I showed that such a mechanism indeed exists but only comes into play after a considerable number of synapses has been potentiated. Thus, adult neurons can sustain a substantial amount of synaptic strengthening but, once a certain threshold of potentiation is exceeded, homeostatic regulation ensures that no further strengthening occurs.

The present study examined the role of N-methyl-D-aspartic acid (NMDA) receptors in synaptic plasticity in regular-spiking cells of rat frontal cortex. Intracortical stimulation, at levels subthreshold for elicitation of action potentials, evoked a late excitatory postsynaptic potential (EPSP) in layer II III neurons that was sensitive to the selective NMDA antagonist -2-amino-5-phosphonovaleric acid (APV). This late EPSP showed marked short-term frequency-dependent depression, suggesting that it is polysynaptic in origin. Polysynaptic late EPSPs were selectively enhanced following high-frequency stimulation. This sustained increase in synaptic efficacy, or long-term potentiation, was expressed in regular spiking cells and appeared to result from activation of NMDA receptors on excitatory interneurons. These data demonstrate the existence of an NMDA-modulated polysynaptic circuit in the neocortex which displays several types of use-dependent plasticity.

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2020.04.19.043117v1?rss=1 Authors: Chindemi, G., Abdellah, M., Amsalem, O., Benavides-Piccione, R., Delattre, V., Doron, M., Ecker, A., King, J. G., Kumbhar, P., Monney, C. C., Perin, R., Rössert, C., Van Geit, W., DeFelipe, J., Graupner, M., Segev, I., Markram, H., Müller, E. B. Abstract: Long-term potentiation (LTP) and long-term depression (LTD) of pyramidal cell connections are among the key mechanisms underlying learning and memory in the brain. Despite their important role, only a few of these connections have been characterized in terms of LTP/LTD dynamics, such as the one between layer 5 thick-tufted pyramidal cells (L5-TTPCs). Comparing the available evidence on different pyramidal connection types reveals a large variability of experimental outcomes, possibly indicating the presence of connection-type-specific mechanisms. Here, we show that a calcium-based plasticity rule regulating L5-TTPC synapses holds also for several other pyramidal-to-pyramidal connections in a digital model of neocortical tissue. In particular, we show that synaptic physiology, cell morphology and innervation patterns jointly determine LTP/LTD dynamics without requiring a different model or parameter set for each connection type. We therefore propose that a similar set of plasticity mechanisms is shared by seemingly very different neocortical connections and that only a small number of targeted experiments is required for generating a complete map of synaptic plasticity dynamics int he neocortex. Copy rights belong to original authors. Visit the link for more info