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Dr. Margaret "Peggy" Goodell is the Vivian L. Smith Chair in Regenerative Medicine and Director of the Stem Cell and Regenerative Medicine Center at Baylor College of Medicine. Her research focuses on HSC regulation and aging. She talks about her discovery of a side population of HSCs, as well as her work on DNMT3A mutation and clonal hematopoiesis of indeterminate potential. Finally, she discusses the rich research environment in Houston.
What if everything you thought you knew about your child's meltdowns was wrong? According to Dr. Caylin Riley, a general pediatrician and mother of 3 highly sensitive children (HSCs), there's a critical piece of the puzzle most parents are missing. It's called the "highly sensitive" trait. And it affects a whopping 20% of all kids. As an HSP (highly sensitive person) herself, Dr. Riley knows firsthand the challenges these unique kids face. She spent years researching to understand what was making life so hard for her oldest child. And what she discovered changed everything. Now, she's on a mission to educate pediatric professionals about this game-changing trait. So that every child can get the support they need to thrive. Book your call with me and my team: https://www.megghanthompsoncoaching.com/talk Connect with Dr. Lange: https://www.pedsforhscs.org/ Want to know if your child is an HSC? Visit here: https://www.megghanthompsoncoaching.com/is-my-child-highly-sensitive? Connect with me: Youtube Channel: https://www.youtube.com/channel/UC1J_DPqWkrqbp7Q8XaGAxxg?view_as=subscriber Website: https://www.megghanthompsoncoaching.com Facebook: https://www.facebook.com/megghanthompsoncoaching Instagram: https://www.instagram.com/megghanthompsoncoaching #HighlySensitiveChild #HighlySensitivePerson #HSC #HSP
HeartSciences is a medical technology company focused on applying innovative AI-based technology to ECGs to expand and improve ECG clinical utility. Millions of ECGs are performed every week. Working to improve healthcare by making it a far more valuable ECG, particularly in frontline or point-of-care clinical settings. With one of the largest libraries of AI-ECG algorithms, the company intends to provide these AI-ECG algorithms on a device agnostic cloud-based solution as well as a low-cost ECG hardware platform. Working with clinical experts, HeartSciences ensures that all solutions are designed to work within existing clinical care pathways, making it easier for clinicians to use AI-ECG technology to improve their patients' care and lead to better outcomes.
This CEO is Using AI to Detect Heart Disease Early – Meet Andrew Simpson CEO of Heart Sciences $HSCS Guest: Andrew Simpson Chairman and CEO / Board Member Heart SciencesTicker: HSCSWebsite:https://heartsciences.com/Bio:Andrew Simpson Chairman and CEO / Board Member Heart SciencesSince March 2022, Andrew Simpson has served as our President and Chief Executive Officer. Mr. Simpson has also served as the Chairman of our Board of Directors, since June 2013, and as a director of our Company since July 2012. Mr. Simpson has over 30 years' experience across a variety of business sectors and sizes. He was Group CEO of The Peel Group from 2006 to 2010, which is a large private company in the UK which, at the time, had approximately $8 billion of business assets across the real estate, ports, airports, energy, media, telecoms and environmental sectors. He was a main board director of Speedy Hire plc from 2003 to 2006 (during which time it became a FTSE 250 company) and during his tenure was Managing Director of its Equipment Rental division which had revenues of approximately $200 million and was also responsible for the Group's development and expansion which included seventeen acquisitions and several non-core divestments. Mr. Simpson qualified as a Chartered Accountant with Price Waterhouse and spent eight years working in investment banking at Rothschild, advising on a wide variety of merger and acquisition transactions, debt and equity fundraisings, IPOs and other advisory assignments. Mr. Simpson graduated with first class honors in 1991 from Sheffield Hallam University in the UK where he received a Bachelor of Arts (honors) in Accounting and Management Control.
Drs. Andrew Elefanty and Elizabeth Ng are Senior Principal Investigator and Principal Investigator, respectively, at the Murdoch Children's Research Institute. In the Blood Development group, they aim to develop innovative cellular therapies for blood and cartilage-related diseases. They talk about their recent study deriving and transplanting HSCs, their work on reporter lines, and their collaborative lab setup.
Discover all of the podcasts in our network, search for specific episodes, get the Optimal Living Daily workbook, and learn more at: OLDPodcast.com. Episode 2342: Melissa Noel Renzi shares valuable insights on nurturing highly sensitive children (HSCs) in a way that embraces their emotional depth. From encouraging the healthy expression of emotions to understanding the importance of alone time, these affirmations support children in turning their sensitivity into a strength, fostering self-acceptance and compassion. Read along with the original article(s) here: https://highlysensitiverefuge.com/highly-sensitive-child-needs-to-hear/ Quotes to ponder: “All of your emotions are acceptable.” “The world needs special people like you.” “Take all the time you need to process.” Episode references: The Highly Sensitive Person: https://www.amazon.com/Highly-Sensitive-Person-Thrive-Overwhelms/dp/0553062182 Learn more about your ad choices. Visit megaphone.fm/adchoices
As an HSC parent, you've probably felt that sinking feeling in the pit of your stomach as summer fades into fall. Another school year...another round of watching your bright, sensitive child struggle to stay afloat in a sea of overstimulation, misunderstanding, and meltdowns. You'd give anything to take away their pain and set them up for the success you KNOW they're capable of. But where do you even start when it feels like nothing works for your child? Here's the good news: there IS a proven path to turn your HSC's school experience from barely surviving to absolutely thriving...and it starts with one simple but powerful skill. It's called RESILIENCE. And it's the key to helping your child navigate challenges, manage big feelings, and show up as their best self in the classroom and beyond. That's why I'm thrilled to invite you to the Success in School for your sensitive kid training - the only training that shows you step-by-step how to cultivate self-advocacy and leave no stone unturned in the school setting for your child Inside this game changing training, you'll discover:
Have you ever wondered if you have a Highly Sensitive Child or HSC? Does your child feel their emotions deeply, and get extremely upset by sad movies or stories?Do they get bothered by the scratchy texture of certain clothes, or find loud noises almost unbearable?If the answer to any of these questions is yes, then chances are, they are highly sensitive.In this episode, I'll outline the top 5 signs or behaviours displayed by HSCs, and the 5 best ways you can support and nurture them.As mentioned in the video, I highly recommend the book "The Highly Sensitive Child" by Dr Elaine Aron, the clinical psychologist who coined the terms Highly Sensitive Child and Highly Sensitive Person back in the mid-1990s. You can get the book on Amazon at the link below. * ➡The Highly Sensitive Child by Elaine AronYou can watch this episode, and most others on YouTube -➡SUBSCRIBE HERE! Let's Connect!➡ Instagram➡Book a FREE connection call#hsp #hsps #hsc #highlysensitivechildren #highlysensitiveperson #parenting #sensorysensitivity*By purchasing through this link, I may receive a small commission, at no extra expense to you.
I'm so happy to have my stress mastery mentor here today!Heidi Hanna, PhD, is one of the most intellectual, intuitive and compassionate people I know, and I'm honored to host her wisdom today. We recorded this interview in March 2020, when we were all in a tailspin and figuring out managing our stress was more important than ever!Fill your brain with Dr. Hanna's research and her personal experience today:At age 10, Heidi was fainting from stress - literally. Find out how to help if this happens to your child, or if they seem to get stomachaches and headaches in uncomfortable situations.Highly sensitive kids actually fare better than other kids if raised in the right environment...but we don't always get that right as you'll hear.What research is showing about how stress-sensitive people's brains work and why Dr. Hanna doesn't love the term "negativity bias," even though it's technically accurate to describe stress-sensitive people.Why HSCs sometimes spiral into thinking like, "It's all my fault, I messed that up, I'm so dumb, and this is why NO ONE LIKES ME!" (I feel like that's my whole high school life!)And more importantly, what parents can do to help kids stop that spiral, appreciate what their reactions are trying to tell them, and reverse the reaction.How we can look at the ways in which our kids (or ourselves) are reacting to stress and understand what recharge techniques they need to get more capacity.Dr. Hanna's encouragement for parents of HSCs and how we can see the positive about our children's personality.Resources We Mention for Managing StressDr. Hanna's websiteDr. Hanna's courses: Stress Mastery Fundamentals 101 and Stress Mastery FoundationsElaine Aron's WebsiteMy Healthy Parenting Handbook Episodes on Stress Mastery: part 1 and part 2My interview with Dr. K, an expert on highly sensitive childrenMy introduction to highly sensitive peopleGoNoodleHeartMath device helps regulate stress responseCheck out today's sponsor, JustThrive, at https://www.kidscookrealfood.com/justhrive, and use Katie15 for 15% off! Kitchen Stewardship Kids Cook Real Food follow Katie on Instagram follow Kids Cook Real Food on Facebook Subscribe to the newsletter to get weekly updates Find the Healthy Parenting Handbook at kidscookrealfood.com/podcast Affiliate links used here. Thanks for supporting the Healthy Parenting Handbook!
Hanna Mikkola, M.D., Ph.D., shares her work in hematopoietic stem cells (HSCs) and their complex renewal process. Her research aims to unravel these cells' behavior in mice and humans, offering potential insights for future medical advancements. Series: "Stem Cell Channel" [Health and Medicine] [Science] [Show ID: 39258]
Hanna Mikkola, M.D., Ph.D., shares her work in hematopoietic stem cells (HSCs) and their complex renewal process. Her research aims to unravel these cells' behavior in mice and humans, offering potential insights for future medical advancements. Series: "Stem Cell Channel" [Health and Medicine] [Science] [Show ID: 39258]
Hanna Mikkola, M.D., Ph.D., shares her work in hematopoietic stem cells (HSCs) and their complex renewal process. Her research aims to unravel these cells' behavior in mice and humans, offering potential insights for future medical advancements. Series: "Stem Cell Channel" [Health and Medicine] [Science] [Show ID: 39258]
Hanna Mikkola, M.D., Ph.D., shares her work in hematopoietic stem cells (HSCs) and their complex renewal process. Her research aims to unravel these cells' behavior in mice and humans, offering potential insights for future medical advancements. Series: "Stem Cell Channel" [Health and Medicine] [Science] [Show ID: 39258]
Hanna Mikkola, M.D., Ph.D., shares her work in hematopoietic stem cells (HSCs) and their complex renewal process. Her research aims to unravel these cells' behavior in mice and humans, offering potential insights for future medical advancements. Series: "Stem Cell Channel" [Health and Medicine] [Science] [Show ID: 39258]
Hanna Mikkola, M.D., Ph.D., shares her work in hematopoietic stem cells (HSCs) and their complex renewal process. Her research aims to unravel these cells' behavior in mice and humans, offering potential insights for future medical advancements. Series: "Stem Cell Channel" [Health and Medicine] [Science] [Show ID: 39258]
Hanna Mikkola, M.D., Ph.D., shares her work in hematopoietic stem cells (HSCs) and their complex renewal process. Her research aims to unravel these cells' behavior in mice and humans, offering potential insights for future medical advancements. Series: "Stem Cell Channel" [Health and Medicine] [Science] [Show ID: 39258]
If you're looking for something unique to add to your aquarium, how about adding an aquatic snake? Many aquatic snake species can thrive in aquariums with compatible fish species. Today's guest is Myke Clarkson, a reptile expert who's making efforts to make aquatic snakes become accessible display animals. Myke also works with Herpetological Conservation International to conserve reptiles. In this episode, we talk about: Mike's background and how he became interested in reptiles and aquatic snakes Monitoring vitamin B1 levels to prevent neurological system breakdown in snakes The importance of monitoring shrimp in aquatic habitats Challenges of keeping aquatic snakes compared to terrestrial snakes Ethical considerations for keeping exotic animals in captivity HSCs and PETA's take on the value of exotic pet ownership Tank requirements of aquatic snakes Fishes compatible with aquatic snakes The distinction between venomous and poisonous snakes Myke's experience of studying aquatic snakes in Indonesia Strategies to replicate the natural environment of snakes in an aquarium Keeping tanks crystal clear vs. allowing some color and imperfection from tannins Having a successful career in TV/entertainment while also doing scientific research The importance of replicating natural environments in aquariums Experimentation and breeding techniques for various snake species Myke's goal to create country-specific biotope aquariums and ponds The shedding process of aquatic snakes Mentioned: Myke Clarkson on Facebook Myke Clarkson (@mykeclarkson) on Instagram Herpetological Conservation International Website Links to resources: Book A Call With Triplett: Call with Triplett The Pond Digger - https://theponddigger.com/ You can also check out The Pond Digger's products at: http://helixpondfiltration.com/ TWT Contractor Circle TWT Contractor Power Circle And follow his adventures in the pond world at: Instagram Facebook TikTok
The holidays are a glorious time of food, family and gratitude! But for parents of HSCs, getting to that table brings worries and anxiety. Last year plays on an endless loop - the dread building each November. Canceled plans, disrupted meals, stress ruining moments meant for bonding. Maybe you're still trying to coax your reluctant social butterfly out from their room to mingle. Or perhaps getting shoes on causes a meltdown because your highly sensitive child struggles with transitions. Maybe that fall sweater Nana made doesn't feel just right, so now there's threats to wear it and tears and screaming ensue. Listen on to learn more. If your child is demonstrating the meltdown cycle, watch our free training here: https://www.megghanthompsoncoaching.com/5-shifts-registration If your teen is struggling to engage with your family, shutting down and struggling with a perfectionism spiral, watch our free training here: https://www.megghanthompsoncoaching.com/tp/5-steps-registration If you're ready to book a call with our team, use this link: https://www.megghanthompsoncoaching.com/pod/book-your-call-now For families with high school aged teens, use this link: https://www.megghanthompsoncoaching.com/tp/teental
I'm not a doctor, but I do understand that what goes into your body affects how you feel. Kids are especially sensitive. Mealtimes can be so hard to manage for HSCs, but keeping nutrition balanced is key for behavior and focus. As Halloween nears, many parents worry. Listen on to learn more. If your child is demonstrating the meltdown cycle, watch our free training here: https://www.megghanthompsoncoaching.com/5-shifts-registration If your teen is struggling to engage with your family, shutting down and struggling with a perfectionism spiral, watch our free training here: https://www.megghanthompsoncoaching.com/tp/5-steps-registration If you're ready to book a call with our team, use this link: https://www.megghanthompsoncoaching.com/pod/book-your-call-now For families with high school aged teens, use this link: https://www.megghanthompsoncoaching.com/tp/teentalk
Do you ever feel like your kid has flaws? If so … I hear you. No parent wants to think there's anything ‘wrong' with their kid, right? Or that they somehow need “fixing.” But unfortunately, society conditions us to think this way. Then, when you add in comments (sometimes well-meaning, sometimes not) from friends and family members, it's easy to see your kids' big emotions as some kind of problem. But what if they weren't a problem at all? What if these were actually reasons to celebrate? This is a reframe we do with our clients. And they LOVE it. Listen on to learn more. If your child is demonstrating the meltdown cycle, watch our free training here: https://www.megghanthompsoncoaching.com/5-shifts-registration If your teen is struggling to engage with your family, shutting down and struggling with a perfectionism spiral, watch our free training here: https://www.megghanthompsoncoaching.com/tp/5-steps-registration If you're ready to book a call with our team, use this link: https://www.megghanthompsoncoaching.com/pod/book-your-call-now For families with high school aged teens, use this link: https://www.megghanthompsoncoaching.com/tp/teentalk
Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2023.07.29.551032v1?rss=1 Authors: Sun, C., Zhou, C., Daneshvar, K., Kratkiewicz, A. J., Ben Saad, A., Hess, A., Chen, J. Y., Pondick, J. V., York, S. R., Li, W., Moran, S. P., Gentile, S., Ur Rahman, R., Li, Z., Sparks, R., Habboub, T., Kim, B.-M., Choi, M., Affo, S., Schwabe, R. F., Popov, Y. V., Mullen, A. C. Abstract: Background & Aims: Fibrosis is the common endpoint for all forms of chronic liver injury, and progression of fibrosis leads to the development of end-stage liver disease. Activation of hepatic stellate cells (HSCs) and their transdifferentiation to myofibroblasts results in the accumulation of the extracellular matrix (ECM) proteins that form the fibrotic scar. Long noncoding (lnc) RNAs regulate the activity of HSCs and may provide targets for fibrotic therapies. Methods: We identified lncRNA TILAM as expressed near COL1A1 in human HSCs and performed loss-of-function studies in human HSCs and liver organoids. Transcriptomic analysis of HSCs isolated from mice defined the murine ortholog of TILAM. We then generated Tilam-deficient GFP reporter mice and quantified fibrotic responses to carbon tetrachloride (CCl4) and choline-deficient L-amino acid defined high fat diet (CDA-HFD). Co-precipitation studies, mass spectrometry, and gene expression analyses identified protein partners of TILAM. Results: TILAM is conserved between human and mouse HSCs and regulates expression of ECM proteins, including collagen. Tilam is selectively induced in murine HSCs during the development of fibrosis in vivo. In both male and female mice, loss of Tilam results in reduced fibrosis in the setting of CCl4 and CDA-HFD injury models. TILAM interacts with promyelocytic leukemia protein (PML) to stabilize PML protein levels and promote the fibrotic activity of HSCs. Conclusion: TILAM is activated in HSCs and interacts with PML to drive the development of liver fibrosis. Depletion of TILAM may serve as a therapeutic approach to combat the development of end stage liver disease. Copy rights belong to original authors. Visit the link for more info Podcast created by Paper Player, LLC
Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2023.07.12.548764v1?rss=1 Authors: Schwartz, L. S., Young, K. A., Stearns, T. M., Boyer, N., Mujica, K. D., Trowbridge, J. J. Abstract: Age-associated clonal hematopoiesis (CH) occurs due to somatic mutations accrued in hematopoietic stem cells (HSCs) that confer a selective advantage in the context of aging. The mechanisms by which CH-mutant HSCs gain this advantage with aging are not comprehensively understood. Using unbiased transcriptomic approaches, we identify Oncostatin M (OSM) signaling as a candidate contributor to aging-driven Dnmt3a-mutant CH. We find that Dnmt3a-mutant HSCs from young mice do not functionally respond to acute OSM stimulation with respect to proliferation, apoptosis, hematopoietic engraftment, or myeloid differentiation. However, young Dnmt3a-mutant HSCs transcriptionally upregulate an inflammatory cytokine network in response to acute OSM stimulation including genes encoding IL-6, IL-1{beta} and TNF. In addition, OSM-stimulated Dnmt3a-mutant HSCs upregulate the anti-inflammatory genes Socs3, Atf3 and Nr4a1, creating a negative feedback loop limiting sustained activation of the inflammatory network. In the context of an aged bone marrow (BM) microenvironment with chronically elevated levels of OSM, Dnmt3a-mutant HSCs upregulate pro-inflammatory genes but do not upregulate Socs3, Atf3 and Nr4a1. Together, our work suggests that chronic inflammation with aging exhausts the regulatory mechanisms in young CH-mutant HSCs that resolve inflammatory states, and that OSM is a master regulator of an inflammatory network that contributes to age-associated CH. Copy rights belong to original authors. Visit the link for more info Podcast created by Paper Player, LLC
While most kids couldn't be more excited for summer … … for HSCs, it can be a time of stress, anxiety, and confusion. That's why I'm discussing today's topic. After working with 600+ families over the past decade, I know just how difficult summer can be. Whether you've got kids off to camp … You're planning days out … Or it's just a break in routine … Listen on to learn more. If your child is demonstrating the meltdown cycle, watch our free training here: https://www.megghanthompsoncoaching.com/5-shifts-registration If your teen is struggling to engage with your family, shutting down and struggling with a perfectionism spiral, watch our free training here: https://www.megghanthompsoncoaching.com/tp/5-steps-registration If you're ready to book a call with our team, use this link: https://www.megghanthompsoncoaching.com/pod/book-your-call-now For families with high school aged teens, use this link: https://www.megghanthompsoncoaching.com/tp/teentalk
Today's topic is a must-listen for any parent thinking of taking their child to therapy. See, while hiring a therapist to help with your kid's needs might seem like a responsible move … Therapy is one of the best ways to make meltdowns worse. That probably sounds controversial. But there are 4 specific reasons I never recommend traditional therapy for HSCs. Listen on to learn more. If your child is demonstrating the meltdown cycle, watch our free training here: https://www.megghanthompsoncoaching.com/5-shifts-registration If your teen is struggling to engage with your family, shutting down and struggling with a perfectionism spiral, watch our free training here: https://www.megghanthompsoncoaching.com/tp/5-steps-registration If you're ready to book a call with our team, use this link: https://www.megghanthompsoncoaching.com/pod/book-your-call-now For families with high school aged teens, use this link: https://www.megghanthompsoncoaching.com/tp/teentalk
No time of the year is “easy” for parents of HSCs. Whether your kid gets super stressed after Christmas … Hates the change in seasons … Or experiences extreme anxiety at the start of a new semester … One time that clients at MTC frequently tell us is a minefield, is the end of the school year. While most kids are excited for the long, school-free days ahead, for HSCs, the change in routine can be triggering. This is why it's so important you know what to do to support them through the transition. Because your actions here can be the difference between a fun-filled summer, full of amazing memories that last a lifetime -- for them and for you … And a summer of meltdowns, arguments, and wishing away the days! If your child is demonstrating the meltdown cycle, watch our free training here: https://www.megghanthompsoncoaching.com/5-shifts-registration If your teen is struggling to engage with your family, shutting down and struggling with a perfectionism spiral, watch our free training here: https://www.megghanthompsoncoaching.com/tp/5-steps-registration If you're ready to book a call with our team, use this link: https://www.megghanthompsoncoaching.com/pod/book-your-call-now For families with high school aged teens, use this link: https://www.megghanthompsoncoaching.com/tp/teentalk
NEWSFLASH: You don't have to ‘cope' with your child being different. Because being different isn't a problem. In fact … It's a GOOD THING. I know that might sound weird. After all, as parents of HSCs, you can often be judged because your kiddo doesn't conform to social norms. But when we work with parents here at MTC, we make sure our clients don't just learn to ‘get by' with an HSC … … but actively CELEBRATE their child's differences. Now, you might be thinking - “But Megghan, my child can be challenging. I love them soooo much … But I don't know how their challenges are an advantage?” If that's you, then listen on to hear how you can dream again! If your child is demonstrating the meltdown cycle, watch our free training here: https://www.megghanthompsoncoaching.com/5-shifts-registration If your teen is struggling to engage with your family, shutting down and struggling with a perfectionism spiral, watch our free training here: https://www.megghanthompsoncoaching.com/tp/5-steps-registration If you're ready to book a call with our team, use this link: https://www.megghanthompsoncoaching.com/pod/book-your-call-now For families with high school aged teens, use this link: https://www.megghanthompsoncoaching.com/tp/teentalk
In this week's episode, we will review patients with chronic ITP having clonal expansions of a specific subset of CD8 T cells, called terminally differentiated effector memory T cells, or TEMRA. Next, for patients with diffuse large B-cell lymphomas, use of a so-called dark-zone signature, previously referred to as the double-hit signature, could help refine prognosis. Finally on today's podcast we'll discuss rethinking the role of hematopoietic stem cells following physiologic emergencies such as acute inflammation and blood loss.
Dr. Shannon McKinney-Freeman is a Principal Investigator and Member at St. Jude Children's Research Hospital. Her lab focuses on the fundamental biology of hematopoietic stem cells (HSCs). She talks about the role of HSCs in sickle cell disease and the state of HSC transplantation research. She also discusses her work on hematopoietic progenitor expansion.
Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2023.04.27.538580v1?rss=1 Authors: Lee, S., Zhan, H. Abstract: Thrombopoietin (TPO) and its receptor MPL play crucial roles in hematopoietic stem cell (HSC) function and platelet production. However, the precise effects of TPO/MPL signaling on HSC regulation in different hematopoietic niches remain unclear. Here, we investigated the effects of TPO/MPL ablation on marrow and splenic hematopoiesis in TPO-/- and MPL-/- mice during aging. Despite severe thrombocytopenia, TPO-/- and MPL-/- mice did not develop marrow failure during a 2-year follow-up. Marrow and splenic HSCs exhibited different responses to TPO/MPL ablation and exogenous TPO treatment. Splenic niche cells compensated for marrow HSC loss in TPO-/- and MPL-/- mice by upregulating CXCL12 levels. These findings provide new insights into the complex regulation of HSCs by TPO/MPL and reveal a previously unknown link between TPO and CXCL12, two key growth factors for HSC maintenance. Understanding the distinct regulatory mechanisms between marrow and spleen hematopoiesis will help develop novel therapeutic approaches for hematopoietic disorders. Copy rights belong to original authors. Visit the link for more info Podcast created by Paper Player, LLC
I recently got pretty mad after a conversation with a client. She overheard a group of moms talking about her child, using words like 'weak, shy, and overly emotional.' It made me upset because these misconceptions about highly sensitive children are simply not true. HSCs are resilient, and with help, they can be sociable and control their emotions. As a parent, it starts with you to take responsibility and help your child. I know some parents want an easy solution, but hearing that it starts with you is empowering. There are ways to help your HSC, but it requires effort on your part. So how do we do that? Listen on to learn how! If your child is demonstrating the meltdown cycle, watch our free training here: https://www.megghanthompsoncoaching.com/5-shifts-registration If your teen is struggling to engage with your family, shutting down and struggling with a perfectionism spiral, watch our free training here: https://www.megghanthompsoncoaching.com/tp/5-steps-registration If you're ready to book a call with our team, use this link: https://www.megghanthompsoncoaching.com/pod/book-your-call-now For families with high school aged teens, use this link: https://www.megghanthompsoncoaching.com/tp/teentalk
In this week's episode we will review optimizing the value of post-transplant lenalidomide maintenance in multiple myeloma. Next, T-cell immunotherapies targeting AML antigens upregulate MHC Class II expression on AML cells. Finally, young bugs rejuvenate old blood: transplanting fecal microbiota from younger mice into older mice improved hematopoietic stem cell function and restored lymphoid differentiation potential.
Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2023.03.27.534417v1?rss=1 Authors: Itkin, T., Houghton, S., Schreiner, R., Lin, Y., Badwe, C., Voisin, V., Murison, A., Seyedhassantehrani, N., Kaufmann, K. B., Garcia-Prat, L., Booth, G. T., Geng, F., Liu, Y., Gomez-Salinero, J. M., Shieh, J.-H., Redmond, D., Xiang, J. Z., Josefowicz, S. Z., Trapnell, C., Spencer, J. A., Zangi, L., Hadland, B., Dick, J. E., Xie, S. Z., Rafii, S. Abstract: Transition between activation and quiescence programs in hematopoietic stem and progenitor cells (HSC/HSPCs) is perceived to be governed intrinsically and by microenvironmental co-adaptation. However, HSC programs dictating both transition and adaptability, remain poorly defined. Single cell multiome analysis divulging differential transcriptional activity between distinct HSPC states, indicated for the exclusive absence of Fli-1 motif from quiescent HSCs. We reveal that Fli-1 activity is essential for HSCs during regenerative hematopoiesis. Fli-1 directs activation programs while manipulating cellular sensory and output machineries, enabling HSPCs co-adoptability with a stimulated vascular niche. During regenerative conditions, Fli-1 presets and enables propagation of niche-derived Notch1 signaling. Constitutively induced Notch1 signaling is sufficient to recuperate functional HSC impairments in the absence of Fli-1. Applying FLI-1 modified-mRNA transduction into lethargic adult human mobilized HSPCs, enables their vigorous niche-mediated expansion along with superior engraftment capacities. Thus, decryption of stem cell activation programs offers valuable insights for immune regenerative medicine. Copy rights belong to original authors. Visit the link for more info Podcast created by Paper Player, LLC
Ever feel like you're walking on eggshells at home? One moment, your HSC is calm, loving and content … The next, they're in full-blown meltdown mode … And then soon after, they totally shut down? If so, let me tell you something - This is expected in the meltdown cycle. In the world of HSCs, going from one emotion to another super quickly isn't uncommon at all. We call this “The shutdown/ irritability cycle.” Just because it's expected though, doesn't make it any easier to deal with. And it definitely doesn't mean you can't do anything about the stress this causes, either. If your child is demonstrating the meltdown cycle, watch our free training here: https://www.megghanthompsoncoaching.com/5-shifts-registration If your teen is struggling to engage with your family, shutting down and struggling with a perfectionism spiral, watch our free training here: https://www.megghanthompsoncoaching.com/tp/5-steps-registration If you're ready to book a call with our team, use this link: https://www.megghanthompsoncoaching.com/pod/book-your-call-now For families with high school aged teens, use this link: https://www.megghanthompsoncoaching.com/tp/teentalk
Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2023.03.20.533430v1?rss=1 Authors: Somuncular, E., Su, T.-Y., Dumral, O., Johansson, A.-S., Luc, S. Abstract: Hematopoiesis is maintained by hematopoietic stem cells (HSCs) that replenish the blood lineages throughout life. It is well-established that the HSC pool is functionally heterogeneous consisting of cells differing in longevity, self-renewal ability, cell proliferation, and lineage differentiation. Although HSCs can be identified through the Lin-Sca-1+c-Kit+CD48-CD34-CD150+ immunophenotype, the cell surface marker combination does not permit the absolute purification of functional HSCs with long-term (LT) reconstituting ability. Prospective isolation of LT HSCs is crucial for mechanistic understanding of the biological functions of HSCs, and for resolving the functional heterogeneity within the HSC population. Here, we show that the combination of CD229 and CD49b cell surface markers within the phenotypic HSC compartment identifies a subset of multipotent progenitor cells with high proliferative activity and short-term reconstituting ability. Thus, functional HSCs can be prospectively isolated by the addition of CD229 and CD49b to conventional HSC markers. Copy rights belong to original authors. Visit the link for more info Podcast created by Paper Player, LLC
Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2023.03.16.532898v1?rss=1 Authors: Watanuki, S., Kobayashi, H., Sugiura, Y., Yamamoto, M., Karigane, D., Shiroshita, K., Sorimachi, Y., Koide, S., Oshima, M., Nishiyama, A., Murakami, K., Miho, H., Tamaki, S., Yamamoto, T., Yabushita, T., Tanaka, Y., Honda, H., Okamoto, S., Goda, N., Tamura, T., Nakamura-Ishizu, A., Suematsu, M., Iwama, A., Suda, T., Takubo, K. Abstract: Metabolic pathways are plastic and rapidly change in response to stress or perturbation. Current metabolic profiling techniques require lysis of many cells, complicating the tracking of metabolic changes over time after stress in rare cells such as hematopoietic stem cells (HSCs). Here, we aimed to identify the key metabolic enzymes that define metabolic differences between steady-state and stress conditions in HSCs and elucidate their regulatory mechanisms. Through quantitative 13C metabolic flux analysis of glucose metabolism using high-sensitivity glucose tracing and mathematical modeling, we found that HSCs activate the glycolytic rate-limiting enzyme phosphofructokinase (PFK) during proliferation and oxidative phosphorylation (OXPHOS) inhibition. Real-time measurement of adenosine triphosphate (ATP) levels in single HSCs demonstrated that proliferative stress or OXPHOS inhibition led to accelerated glycolysis via increased activity of PFKFB3, the enzyme regulating an allosteric PFK activator, within seconds to meet ATP requirements. Furthermore, varying stresses differentially activated PFKFB3 via PRMT1-dependent methylation during proliferative stress and via AMPK-dependent phosphorylation during OXPHOS inhibition. Overexpression of Pfkfb3 induced HSC proliferation and promoted differentiated cell production, whereas inhibition or loss of Pfkfb3 suppressed them. This study reveals the flexible and multilayered regulation of HSC metabolism to sustain hematopoiesis under stress and provides techniques to better understand the physiological metabolism of rare hematopoietic cells. Copy rights belong to original authors. Visit the link for more info Podcast created by Paper Player, LLC
Ever feel like no matter what you do, you just can't get through to your kid? You've read all the books … Tried all the tactics … And diligently stick to everything you should be doing … But whenever you try to speak to them about emotions, they clam up? If so … Welcome to the world of parenting an HSC :) I'm not saying all HSCs are difficult to talk to about emotions. But the majority are. And that can lead parents to feeling disheartened, disillusioned, and like there's no point even trying. Many think outsourcing emotional regulation to a therapist is the only choice. So they ask around, go for multiple assessments, and spend a small fortune on sessions for their kiddo, which do … Absolutely nothing! See, there can be a time and a place for therapy. But emotional regulation isn't it. Listen on to learn more! If your child is demonstrating the meltdown cycle, watch our free training here: https://www.megghanthompsoncoaching.com/5-shifts-registration If your teen is struggling to engage with your family, shutting down and struggling with a perfectionism spiral, watch our free training here: https://www.megghanthompsoncoaching.com/tp/5-steps-registration If you're ready to book a call with our team, use this link: https://www.megghanthompsoncoaching.com/pod/book-your-call-now For families with high school aged teens, use this link: https://www.megghanthompsoncoaching.com/tp/teentalk
As parents, our number one priority is to make sure our kids feel safe at all times. But when you're parenting an HSC, that's easier said than done. We don't want to see our kiddo hurting, and struggling to express their emotions. And we definitely don't want them to feel unsafe. And so more often than not, we become their safe space. Now, a ‘safe space' can mean a lot of different things for different HSCs. In most cases, if you can provide whatever safe space is needed FOR THEM, that particular meltdown will end sooner. But there's a problem … Because while this strategy might be useful in the moment, it doesn't do a lot to end the long-term meltdown cycle. In fact, chances are, you're making things worse. Listen on to learn more. If your child is demonstrating the meltdown cycle, watch our free training here: https://www.megghanthompsoncoaching.com/5-shifts-registration If your teen is struggling to engage with your family, shutting down and struggling with a perfectionism spiral, watch our free training here: https://www.megghanthompsoncoaching.com/tp/5-steps-registration If you're ready to book a call with our team, use this link: https://www.megghanthompsoncoaching.com/pod/book-your-call-now For families with high school aged teens, use this link: https://www.megghanthompsoncoaching.com/tp/teentalk
Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2023.02.08.527614v1?rss=1 Authors: Ibneeva, L., Singh, S. P., Sinha, A., Eski, S. E., Wehner, R., Rupp, L., Perez-Valencia, J. A., Gerbaulet, A., Reinhardt, S., Wobus, M., Bonin, M., Sancho, J., Lund, F. E., Dahl, A., Schmitz, M., Bornhaeuser, M., Chavakis, T., Wielockx, B., Grinenko, T. Abstract: A subpopulation of deeply quiescent, so-called dormant hematopoietic stem cells (dHSCs) resides at the top of the hematopoietic hierarchy and serves as a reserve pool for HSCs possessing the greatest long-term blood repopulation capacity. The state of dormancy protects the HSC pool from exhaustion throughout life, however excessive dormancy may block an efficient response to hematological stresses. The mechanisms of HSC dormancy remain elusive, mainly due to the absence of surface markers that allow dHSC prompt isolation. Here, we identify CD38 as a novel surface marker for murine dHSCs that is broadly applicable. Moreover, we demonstrate that cyclic adenosine diphosphate ribose (cADPR), the product of CD38 cyclase activity, regulates the expression of the transcription factor c-Fos by increasing cytoplasmic Ca2+ concentration. Strikingly, we uncover that c-Fos drives HSCs dormancy through the induction of the cell cycle inhibitor p57Kip2. Moreover, we found that CD38 ecto-enzymatic activity at the neighboring CD38-positive cells can promote human HSC quiescence. Together, CD38/cADPR/Ca2+/cFos/p57Kip2 axis maintains HSC dormancy. Pharmacological manipulations of this pathway can provide new strategies to expand dHSCs for transplantation or to activate them during hematological stresses. Copy rights belong to original authors. Visit the link for more info Podcast created by Paper Player, LLC
Is your kid struggling in school? Maybe refusing to do homework? Not playing or socializing well with others? Cruising alone fine one minute, then in full-on meltdown mode the next? If so, I'm guessing you've considered getting them a psych eval. Or maybe their school's suggested this is the next step to figure out what's “wrong.” But could a psych eval do more harm than good? Spoiler alert … Yes it can! I don't say that to scare you. I say that because it's important for you to know, psych evals can be confusing for parents HSCs. They can lead to more anxiety, more uncertainty, and STOP you from getting them the help they need. Listen on to learn why … Join our FREE live event, Friday February 3rd at 4pm est. Register to reserve your spot now: www.megghanthompsoncoaching.com/school
Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2023.01.24.525321v1?rss=1 Authors: Rundberg Nilsson, A. J., Xian, H., Shalapour, S., Cammenga, J., Karin, M. Abstract: Inflammatory mediators induce emergency myelopoiesis and cycling of adult hematopoietic stem cells (HSCs) through incompletely understood mechanisms. To suppress the unwanted effects of inflammation and preserve its beneficial outcomes, the mechanisms by which inflammation affects hematopoiesis need to be fully elucidated. Rather than focusing on specific inflammatory stimuli, we here investigated the role of transcription factor Interferon (IFN) regulatory factor 1 (IRF1), which receives input from several inflammatory signaling pathways. We identify IRF1 as a master HSC regulator. IRF1 loss impairs HSC self-renewal, increases stress-induced cell cycle activation, and confers apoptosis resistance. Transcriptomic analysis revealed an aged, inflammatory signature devoid of IFN signaling with reduced megakaryocytic/erythroid priming and antigen presentation in IRF1-deficient HSCs. Finally, we conducted IRF1-based AML patient stratification to identify groups with distinct proliferative, survival and differentiation features, overlapping with our murine HSC results. Our findings position IRF1 as a pivotal regulator of HSC preservation and stress-induced responses. Copy rights belong to original authors. Visit the link for more info Podcast created by Paper Player, LLC
Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2023.01.23.525185v1?rss=1 Authors: Calderon, A., Mestvirishvili, T., Boccalatte, F., Ruggles, K., David, G. Abstract: To maintain blood homeostasis, millions of terminally differentiated effector cells are produced every day. At the apex of this massive and constant blood production lie hematopoietic stem cells (HSCs), a rare cell type harboring unique self-renewal and multipotent properties. A key feature of HSCs is their ability to temporarily exit the cell cycle in a state termed quiescence. Defective control of cell cycle progression can eventually lead to bone marrow failure or malignant transformation. Recent work in embryonic stem cells has suggested that cells can more robustly respond to differentiation cues in the early phases of the cell cycle, owing to a discrete chromatin state permissive to cell fate commitment. However, the molecular mechanisms tying cell cycle re-entry to cell fate commitment in adult stem cells such as HSCs remain elusive. Here, we report that the chromatin-associated Sin3B protein is necessary for HSCs' commitment to differentiation, but dispensable for their self-renewal or survival. Transcriptional profiling of hematopoietic stem and progenitor cells (HSPCs) genetically inactivated for Sin3B at the single cell level reveals aberrant cell cycle gene expression, correlating with the defective engagement of discrete signaling programs. In particular, the loss of Sin3B in the hematopoietic compartment results in aberrant expression of cell adhesion molecules and essential components of the interferon signaling cascade in LT-HSCs. Finally, chromatin accessibility profiling in LT-HSCs suggests a link between Sin3B-dependent cell cycle progression and priming of hematopoietic stem cells for differentiation. Together, these results point to controlled progression through the G1 phase of the cell cycle as a likely regulator of HSC lineage commitment through the modulation of chromatin features. Copy rights belong to original authors. Visit the link for more info Podcast created by Paper Player, LLC
Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2023.01.21.525037v1?rss=1 Authors: Martinez, D., Maldonado, V., Perez, C., Yanez, R., Candia, V., Kalaidzidis, Y., Zerial, M., Morales-Navarrete, H., Segovia-Miranda, F. Abstract: Three dimensional (3D) geometrical models are not only a powerful tool for quantitatively characterizing complex tissues but also useful for probing structure-function relationships in a tissue. However, these models are generally incomplete due to experimental limitations in acquiring multiple ( greater than 4) fluorescent channels simultaneously. Indeed, predictive geometrical and functional models of the liver have been restricted to few tissue and cellular components, excluding important cellular populations such as hepatic stellate cells (HSCs) and Kupffer cells (KCs). Here, we performed deep-tissue immunostaining, multiphoton microscopy, deeplearning techniques, and 3D image processing to computationally expand the number of simultaneously reconstructed tissue structures. We then generated a spatio-temporal singlecell atlas of hepatic architecture (Hep3D), including all main tissue and cellular components at different stages of post-natal development in mice. We used Hep3D to quantitatively study 1) hepatic morphodynamics from early post-natal development to adulthood, and 2) the structural role of KCs in the murine liver homeostasis. In addition to a complete description of bile canaliculi and sinusoidal network remodeling, our analysis uncovered unexpected spatiotemporal patterns of non-parenchymal cells and hepatocytes differing in size, number of nuclei, and DNA content. Surprisingly, we found that the specific depletion of KCs alters the number and morphology of the HSCs. These findings reveal novel characteristics of liver heterogeneity and have important implications for both the structural organization of liver tissue and its function. Our next-gen 3D single-cell atlas is a powerful tool to understand liver tissue architecture, under both physiological and pathological conditions. Copy rights belong to original authors. Visit the link for more info Podcast created by Paper Player, LLC
Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2023.01.08.523142v1?rss=1 Authors: Collins, A., Scott, R., Wilson, C. L., Abbate, G., Ecclestone, G. B., Biddles, D., Oakley, F., Mann, J., Mann, D. A., Kenneth, N. S. Abstract: Liver fibrosis is the excessive accumulation of extracellular matrix proteins that occurs in most types of chronic liver diseases. Fibrosis is associated with the activation of hepatic stellate cells (HSCs) which transdifferentiate into a myofibroblast like phenotype that is contractile, proliferative and profibrogenic. Hypoxia-inducible factor 1 (HIF1), an oxygen-sensitive transcription factor, is elevated during HSC activation and promotes the expression of profibrotic mediator HIF target genes. HIF activation during HSC activation can by either due to localised decreases in oxygen levels, or through oxygen-independent mechanisms that are not completely understood. Here we describe a role for the deubiquitinase UCHL1 in regulating HIF levels and activity during HSC activation and liver fibrosis. Increased HIF1 expression correlated with induction of UCHL1 mRNA and protein with HSC activation. Genetic deletion or chemical inhibition of UCHL1 impaired HIF activity through reduction of HIF1 levels. UCHL1 specifically cleaves the degradative ubiquitin chains from HIF1 leading to increased HIF1 levels, even in sufficiently oxygenated cells. Furthermore, our mechanistic studies have shown that UCHL1 elevates HIF activity through specific cleavage of degradative ubiquitin chains, elevates levels of pro-fibrotic gene expression and increases proliferation rates. These results demonstrate how small molecule inhibitors of DUBs can modulate the activity of HIF transcription factors in liver disease. Furthermore, inhibition of HIF activity via modulation of the ubiquitin-proteasomal degradation pathway may represent a therapeutic opportunity with other HIF-related pathologies. Copy rights belong to original authors. Visit the link for more info Podcast created by Paper Player, LLC
In the Season 3 NAFLD Year-in-Review conversations series, Surfers Jörn Schattenberg, Louise Campbell and Roger Green embark on a string of interviews with a handful of Key Opinion Leaders who made headlines and advances in Fatty Liver disease in 2022. In this exclusive segment, Scott Friedman joins to discuss his perspective on what has been an eye-opening year for liver science. Scott prefaces this session with the idea that in the absence of an approved drug, the field has responded with an urgency to develop improved pathways and targets. This urgency is coupled with progressive efforts to modify and improve clinical trials and endpoints, and noninvasive markers to make those trials more streamlined and accessible. From here he introduces illuminating findings on genetic variants associated with protection from Fatty Liver disease. An attention-grabbing conclusion: rare germline mutations in the CIDEB gene conferred substantial protection from liver disease. Scott states the implications are twofold. This summons recognition of the power of genetics in uncovering new pathways. This also highlights the complexities of a disease featuring genetic variants that may variably contribute to the likelihood that a patient with obesity develops NASH. He suggests that broadly, the challenge in NASH is sifting through an enormous palette of drivers - be it genetic, derived from the microbiome, or attributable to diabetes and the consequences of metabolic syndrome. All of which influence how we decide to establish and prioritize strategies for the discovery of new therapeutic targets. Reinforcing his recent comment on the podcast that gene therapy has effectively arrived, he notes the speed by which genetic information can translate into real therapies. Continuing on the theme of scientific advancements, Scott points to papers emerging from the Columbia University lab of Robert Schwabe. The aim of the Schwabe lab is to understand the regulation of wound healing responses to chronic liver injury with a specific focus on how the activation of hepatic stellate cells (HSCs) promotes liver fibrosis and liver carcinogenesis. Scott raises a spate of compelling questions in response to Schwabe's interrogation and subsequent findings on functions of HSCs, the main source of liver fibroblasts, during hepatocarcinogenesis. He accredits the feasibility of these studies back to the emerging availability of single cell sequencing technologies. Once again, Scott reveals his acceptance that the “devil is in the details,” alluding to the back-and-forth switching capacity of particular cellular functions. By identifying the transcription factor that drives this switching to a protective role, Scott suggests there is potential to enhance the expression of that transcription factor using gene therapy. Whether we can realistically expect to change the behavior of cells in the setting of an injured liver through the manipulation of gene expression is something to look forward to. “The best is yet to come.”The group breaks into a brief discussion around the stepwise commercial progression and eventual success of Hepatitis C treatment. As the interview winds down, Scott returns to one last paper of interest stemming from his own laboratory and set to be published in the imminent future. The focus of the paper postulates stage-specific therapies as NASH develops. While admittedly “a long way off” from a clinical setting, this project framework injects Scott with invigoration to address possibly the largest unmet need of patients who have or are approaching cirrhosis. Surf on to his upcoming NASH-TAG talk for a more explicit demonstration of this unfolding story.
So you ‘survived' the first 12 or so Christmases of your HSC's life, and now you're looking forward to a smoother, less stressful holiday season? Well … Good luck with that! Unfortunately, parenting a sensitive child at any age comes with its challenges. And while those holiday challenges might be different now than when they were younger … You're still going to have your patience tested this holiday season. That's why I wanted to dedicate this week's topic to any parent of a teen who's worried about the month ahead. Now, a lot of what I speak about in terms of holidays and HSCs in general still applies for teens. Routine is important. And you definitely don't want to overhaul your plans, just because you're worried of how they might react, But there are also special considerations that only apply to teens. Listen on to learn more! If your child is demonstrating the meltdown cycle, watch our free training here: https://www.megghanthompsoncoaching.com/5-shifts-registration If your teen is struggling to engage with your family, shutting down and struggling with a perfectionism spiral, watch our free training here: https://www.megghanthompsoncoaching.com/tp/5-steps-registration If you're ready to book a call with our team, use this link: https://www.megghanthompsoncoaching.com/pod/book-your-call-now For families with high school aged teens: https://www.megghanthompsoncoaching.com/tp/teentalk
Hear how Ashley was able to go from keeping the peace by giving in to CREATING PEACE in her own home...with TWO HSCs. Listen on to learn how she was able to do this with the help of MTC! If your child is demonstrating the meltdown cycle, watch our free training here: https://www.megghanthompsoncoaching.com/5-shifts-registration If your teen is struggling to engage with your family, shutting down and struggling with a perfectionism spiral, watch our free training here: https://www.megghanthompsoncoaching.com/tp/5-steps-registration If you're ready to book a call with our team, use this link: https://www.megghanthompsoncoaching.com/pod/book-your-call-now For families with high school aged teens: https://www.megghanthompsoncoaching.com/tp/teentalk
As a society, we love to put labels on things. Whether it's labeling people as ‘good' or ‘bad' … Labeling foods as ‘healthy' or ‘unhealthy' … Or putting labels on kids who don't conform exactly to what's expected of them. This comes up a lot in my private practice. Parents come in because their kiddo has been displaying HSC-like behavior, and they think they need a diagnosis … I can understand why getting a diagnosis might give parents some comfort. After all, if you went to the doctor with unexplained stomach pain, you'd want them to give you a concrete reason for that, right? You'd want a diagnosis. But in HSCs, most of the time, a diagnosis has a negative impact on their self-esteem and on their future. Listen on to learn more! If your child is demonstrating the meltdown cycle, watch our free training here: https://www.megghanthompsoncoaching.com/5steps If your teen is struggling to engage with your family, shutting down and struggling with a perfectionism spiral, watch our free training here: https://www.megghanthompsoncoaching.com/5steps_teen If you're ready to book a call with our team, use this link: megghanthompsoncoaching.com/call For families with high school aged teens: www.megghanthompsoncoaching.com/teentalk
Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2022.11.17.516971v1?rss=1 Authors: Thambyrajah, R., Fadlullah, Z., Proffitt, M., Neo, W. H., Guillen, Y., Casado-Pelaez, M., Herrero-Molinero, P., Brujas, C., Castelluccio, N., Gonzalez, J., Iglesias, A., Marruecos, L., Ruiz-Herguido, C., Esteller, M., Mereu, E., Lacaud, G., Espinosa, L., Bigas, A. Abstract: Recent findings are challenging the classical hematopoietic model in which long-term hematopoietic stem cells (LT-HSC) are the base of the hematopoietic system. Clonal dynamics analysis of the hematopoietic system indicate that LT-HSC are not the main contributors of normal hemapoiesis in physiological conditions and the hematopoietic system is mainly maintained by multipotent progenitors (MPPs, hereafter HPC) and LT-HSCs are mostly in a non-active state. The first HSCs emerge from the aorta-gonad and mesonephros (AGM) region along with hematopoietic progenitors (HPC) within hematopoietic clusters. Molecular pathways that determine the HSC fate instead of HPC are still unknown, although inflammatory signaling, including NF-KB has been implicated in the development of HSCs. Here, we identify a chromatin binding function for IKB (also known as the inhibitor of NF-KB) that is Polycomb repression complex 2 (PRC2)- dependent and specifically determines dormant vs proliferating HSCs from the onset of their emergence in the AGM. We find a specific reduction of LT-HSCs in the IKB knockout new-born pups. This defect is manifested at the FL stage already, and traceable to the first emerging HSCs in the E11.5 AGM, without affecting the general HPC population. IKB-deficient LT-HSCs express dormancy signature genes, are less proliferative and can robustly respond to activation stimuli such as in vitro culture and serial transplantation. At the molecular level, we find decreased PRC2-dependent H3K27me3 at the promoters of several retinoic acid signaling elements in the IKB- deficient aortic endothelium and E14.5 FL LT-HSCs. Additionally, IKB binding itself is found in the promoters of retinoic acid receptors rar in the AGM, and rar{gamma} in the LT-HSC of FL. Overall, we demonstrate that the retinoic acid pathway is over-activated in the hematopoietic clusters of IKB-deficient AGMs leading to premature dormancy of LT- HSCs that persists in the FL LT-HSCs. Copy rights belong to original authors. Visit the link for more info Podcast created by Paper Player, LLC
Do you ever feel embarrassed by your kid's behavior? If so … You're not alone. Being embarrassed is a normal part of parenting. But with an HSC, that embarrassment can be on another level. Not only do embarrassing situations crop up more frequently … They can also be way more intense. So it's normal to spend a lot of your time feeling red-faced, ashamed, and worrying that everyone's looking at you. Or worse … Judging you. And, while most kids grow out of this naturally, that's definitely not the case with HSCs. Listen on to learn why! If your child is demonstrating the meltdown cycle, watch our free training here: https://www.megghanthompsoncoaching.com/5steps If your teen is struggling to engage with your family, shutting down and struggling with a perfectionism spiral, watch our free training here: https://www.megghanthompsoncoaching.com/5steps_teen If you're ready to book a call with our team, use this link: megghanthompsoncoaching.com/call
In today's episode, Chris welcomes Sebastian Brunemeier, a biotech VC and company builder focused on longevity and regenerative medicine. Sebastien is the Co-Founder and General Partner of Healthspan Capital, a longevity VC firm that invests in biotechnology startups developing therapies to slow or reverse aging, and the CEO and Co-Founder of ImmuneAGE Pharma, a new company based on a drug discovery platform for immune rejuvenation. At Healthspan Capital, Sebastian is looking to invest in fellow “true believers” in longevity and regenerative medicine—companies that understand the importance of aging as a focus for biotech and won't pivot away from longevity as a focus. Sebastian's newest venture, ImmuneAge Pharma, is focused on rejuvenating the immune system. With over 100 years of combined drug discovery expertise, the company aims to systematically identify small molecules that rejuvenate hematopoietic stem cells (HSCs). As Sebastian points out, the goal of longevity and regenerative medicine is not to extend lifespan at any cost, but rather to increase quality-adjusted life years. The Finer Details of This Episode: Healthspan Capital and the importance of aging as a focus for biotech Investing in fellow believers who won't pivot away from aging Alzheimer's drug development and the “amyloid mafia” ImmuneAge Pharma and immune aging The importance of restoring immune function Key misunderstandings of regenerative medicine Quotes: “We noticed that there was a gap in the market for more traditional structured VC, the longevity biotech space, and actually I and my co-founders launched Healthspan because we were looking to invest our own money into a broadly diversified portfolio and a long bio space, and there was no way to do it. So we had to create it ourselves.” “I would argue that if you have a drug that enhances robustness and resilience and extends lifespan, and it works in multiple different animal models and disease, contrived or not, that is a much stronger preclinical signal for efficacy down the road.” “We've already found a couple of interesting molecules that we're doing med chem on to improve their properties that identify the molecular target. And so, we're hopeful that we'll find a whole pipeline of assets that rejuvenate the immune system.” “If we can dramatically improve outcomes for patients who receive chemo, that would be an absolute home run. It's a huge unmet market need. And this is something that I would want for myself and my friends and family to be available.” “We want to gently, slowly replace the existing HSCs in the niche.” “I'm primarily not in this for the money at this point. I am in this to extend healthy lifespan in myself and my loved ones and the world, and showing Big Pharma that there is a new way, another world is possible. We can actually treat disease at the root cause: the fundamental biology of aging.” “We want to compress the time in which we're spending years in poor health at the end of life, which is very expensive for the whole world and the whole system. ” Links: Email questions, comments, and feedback to podcast@bioagelabs.com Translating Aging on Twitter:https://twitter.com/BioAgePodcast ( @bioagepodcast) BIOAGE Labs Websitehttps://bioagelabs.com/ ( BIOAGELabs.com) BIOAGE Labs Twitterhttps://twitter.com/bioagelabs?lang=en ( @bioagelabs) BIOAGE Labshttps://www.linkedin.com/company/bioage-labs/ ( LinkedIn) Healthspan Capital Website: https://www.healthspancapital.vc/ (https://www.healthspancapital.vc/) Sebastian A. Brunemeier on LinkedIn: https://www.linkedin.com/in/sebastianaguiar/ (https://www.linkedin.com/in/sebastianaguiar/) Longevity Marketcap Newsletter: https://sub.longevitymarketcap.com/ (https://sub.longevitymarketcap.com/) The DeSci movement: https://ethereum.org/en/desci/ (https://ethereum.org/en/desci/)
Do you ever wonder if your kid is REALLY having a problem at school … … or if what they're going through is just a regular struggle that might pass in time? Parenting a sensitive child is tough. You know they have needs other kids don't. And that you need to bring your A-game, every single day. But still, sometimes there's that creeping doubt - “Should I be doing more … Or do I need to let them figure some of this out on their own?” You know me - In my coaching practice, we're all about giving HSCs the tools, skills and resilience they need to handle whatever life throws at them. But sometimes, you gotta step in. Especially if they're really having problems at school. The question is - How do you know? Well, there's 3 things to look out for. Listen on to learn more. If your child is demonstrating the meltdown cycle, watch our free training here: https://www.megghanthompsoncoaching.com/5steps If your teen is struggling to engage with your family, shutting down and struggling with a perfectionism spiral, watch our free training here: https://www.megghanthompsoncoaching.com/5steps_teen If you're ready to book a call with our team, use this link: megghanthompsoncoaching.com/call
Waiting with baited breath trying to suck the life out of this last week before you have to be a ‘mean parent' again. What if there's a better way? There's no doubt about it, HSCs thrive on having a regular routine. And that's all well and good … But sometimes, try as you might, routine isn't something you can control. You know this as well as anyone, but small changes can bring about BIG reactions in sensitive kids. They can get stuck in overwhelm, descend into a shutdown cycle, or go into full-on meltdowns, just from the tiniest change in schedule. Meaning for many parents, getting back into a routine post-Summer is a nightmare. And what might seem like a small change to you (or even to most kids) feels like their world is ending to an HSC. Listen on to learn why. If your child is demonstrating the meltdown cycle, watch our free training here: https://www.megghanthompsoncoaching.com/5steps If your teen is struggling to engage with your family, shutting down and struggling with a perfectionism spiral, watch our free training here: https://www.megghanthompsoncoaching.com/5steps_teen If you're ready to book a call with our team, use this link: megghanthompsoncoaching.com/call
Alex Carlson recaps the action in the OTC, NASDAQ, and NYSE. He covers SIRC, TOMDF, ILAG, HKD, TOP, MEGL, HSCS, AIHS, and GDRX. All stocks have been discussed on https://insiderfinancial.com/ To sign up for our FREE alerts and eBook, go to: https://signup.insiderfinancial.com/ To sign up for FREE stocks and trade OTC on WeBull, go to: https://a.webull.com/i/insiderfinancial Disclosure: We have no business relationship with any company whose stock is mentioned in this video. Insider Financial is not an investment advisor; this video does not provide investment advice. Always do your research, make your own investment decisions, or consult with your nearest financial advisor. This video is not a solicitation or recommendation to buy, sell, or hold securities. This video is our opinion, is meant for informational and educational purposes only, and does not provide investment advice. Past performance is not indicative of future performance. For more information, please read our full disclaimer: https://insiderfinancial.com/disclaimer/ sirc stock, tomdf stock, ilag stock, hscs stock, aihs stock, top stock, hkd stock, megl stock, gdrx stock, otc stocks, otc stocks list, penny stocks, penny stocks list, NASDAQ penny stocks, NYSE stocks, NYSE penny stocks #pennystocks #stocks #nasdaq
Alex Carlson recaps the action in the OTC, NASDAQ, and NYSE. He covers CYDY, TOMDF, SIRC, HKD, AMTD, APDN, MOHO, QNRX, KSPN, ENVB, HSCS, PSTX, GRNQ, XCUR, YELL, SYN, SYRS, GRNA, LEJU, and MOTS. All stocks have been discussed on https://insiderfinancial.com/ To sign up for our FREE alerts and eBook, go to: https://signup.insiderfinancial.com/ To sign up for FREE stocks and trade OTC on WeBull, go to: https://a.webull.com/i/insiderfinancial Disclosure: We have no business relationship with any company whose stock is mentioned in this video. Insider Financial is not an investment advisor; this video does not provide investment advice. Always do your own research, make your own investment decisions, or consult with your nearest financial advisor. This video is not a solicitation or recommendation to buy, sell, or hold securities. This video is our opinion, is meant for informational and educational purposes only, and does not provide investment advice. Past performance is not indicative of future performance. For more information, please read our full disclaimer: https://insiderfinancial.com/disclaimer/ cydy stock, tomdf stock, sirc stock, hkd stock, amtd stock, apdn stock, moho stock, qnrx stock, kspn stock, envb stock, hscs stock, pstx stock, grnq stock, xcur stock, yell stock, syn stock, syrs stock, grna stock, leju stock, mots stock, otc stocks, otc stocks list, penny stocks, penny stocks list, NASDAQ penny stocks, NYSE stocks, NYSE penny stocks #pennystocks #stocks #nasdaq
It's a common phrase in positive parenting, isn't it? Has an awesome ring to it– If your child feels better about themselves, their lives, their relationships, they will succeed. This promise leaves you holding the bag when you're parenting a Highly Sensitive Child stuck in the meltdown cycle, however, so let's cover why this parenting phrase is FALSE for your kid. HSCs want to feel better, of course, but they are missing the capacity to generalize their skills. So, when you follow the premise of just creating more positivity in your home to solve the meltdown cycle, you are still parenting your child like a non-Highly Sensitive Child. Tune in to learn more. If your child is demonstrating the meltdown cycle, watch our free training here: https://www.megghanthompsoncoaching.com/5steps If your teen is struggling to engage with your family, shutting down and struggling with a perfectionism spiral, watch our free training here: https://www.megghanthompsoncoaching.com/5steps_teen If you're ready to book a call with our team, use this link: megghanthompsoncoaching.com/call
Melina Marchetta's coming-of-age classic about “the curse”, boys, the HSCs and growing up an “ethnic” in Australia turns 30 this year. A new stage adaptation of Looking for Alibrandi written by Vidya Rajan and directed by Stephen Nicolazzo, reframes the beloved story with a sharper focus on the three Alibrandi women. How does the story hold up in 2022, and what's the symbolism of all those tomatoes? Five years ago, Yassmin Abdel Magied's six-word an Anzac Day Facebook post set off a culture war that ultimately led the engineer and social advocate to leave Australia for the UK. BW checks in with Yassmin, whose new essay collection, Talking About a Revolution, looks back at the events of 2017 and beyond. Show Notes: Looking for Alibrandi on Stage or Book Yassmin Abdel-Magied - Talking About a Revolution
Melina Marchetta's coming-of-age classic about “the curse”, boys, the HSCs and growing up an “ethnic” in Australia turns 30 this year. A new stage adaptation of Looking for Alibrandi written by Vidya Rajan and directed by Stephen Nicolazzo, reframes the beloved story with a sharper focus on the three Alibrandi women. How does the story hold up in 2022, and what's the symbolism of all those tomatoes? Five years ago, Yassmin Abdel Magied's six-word an Anzac Day Facebook post set off a culture war that ultimately led the engineer and social advocate to leave Australia for the UK. BW checks in with Yassmin, whose new essay collection, Talking About a Revolution, looks back at the events of 2017 and beyond.Show Notes:Looking for Alibrandi on Stage or BookYassmin Abdel-Magied - Talking About a Revolution
When you're struggling with the daily meltdown cycle, it's easy to feel exhausted every day. You're wiped out, a fraction of yourself, and you find yourself wondering: is this my future forever? Without a plan, it's frightening because when you picture giving up as an option, you know that thought wouldn't actually cross your mind if it wasn't a real possibility. Join me on today's episode as I discuss the 3 biggest decisions parents make to break out of the cycle of feeling like giving up. If your child is demonstrating the meltdown cycle, watch our free training here: https://www.megghanthompsoncoaching.com/5steps If your teen is struggling to engage with your family, shutting down and struggling with a perfectionism spiral, watch our free training here: https://www.megghanthompsoncoaching.com/5steps_teen If you're ready to book a call with our team, use this link: megghanthompsoncoaching.com/call
https://marklwhite.com Most people are not aware that we all experience a huge metabolic switch between the ages of 15-25 that shifts our bodies from growth and into aging. Although experts have not been able to identify the cause behind this switch, leaders in the field of regenerative medicine, like Dr. Ian White, are on a mission to discover it with the goal of ending aging forever. During this fascinating episode, Dr. White and I cover the topics below: - The science of aging and age-related disease - Why we age and why we don't have to - The Unified Theory of LIFE: Evolution, aging and the future of regenerative medicine - Editing genes to rid disease - Billionaires & Mars: What's going to happen to the rest of us? *About Our Guest* Dr. White is an expert in the field of regenerative medicine and stem cell biology with over 20 years experience working in academia and industry. Dr. White received his B.S. from Liverpool John Moores University and his M.S. from the famed Liverpool School of Tropical Medicine in England before being hired at Dartmouth College in the United States to study the genetics of gamete biology. In August of 2000 Dr. White was recruited to Harvard University to work with hematopoietic stem cells (HSCs) and immune cell biology under the mentorship of the world-renowned Dr. Laurie Glimcher, where he co-authored several peer-reviewed scientific publications. Dr. White went on to receive his Ph.D. from the Ansary Stem Cell Institute, division of Regenerative Medicine at Cornell University under the guidance of Howard Hughes investigator Dr. Shahin Rafii. During this time Dr. White developed an in vitro method for culture expanding autologous HSCs in an artificial vascular niche for the treatment of cancer patients who have undergone bone marrow ablation following chemo- or radio-therapy. This technology has since gone on to be commercialized by Cornell and the company, Angiocrine, LLC, has become one of the top two HSC expansion companies in the US. Following the completion of his Ph.D., Dr. White spent time as a post-doctoral research scientist in the embryonic stem cell (ESC) laboratory of Dr. Stephen Dalton, whose pioneering work led to the inclusion of c-myc as one of the four factors used by Nobel award-winning Dr. Shinya Yamanaka in the generation of induced pluripotent stem cells (iPSCs). Subsequently, Dr. White relocated to the Interdisciplinary Stem Cell Institute at the University of Miami Miller School of Medicine. Dr. White published ground-breaking research in the field of regenerative medicine alongside Dr. Joshua Hare, including a book chapter on the use of Mesenchymal Stem Cells (MSCs) in cardiac regeneration. In 2015, Dr. White's work on the regeneration of the heart was featured on the cover of Circulation Research, one of the top peer-reviewed journals for cardiovascular medicine in the world. In 2016. Dr. White was honored with an award for the “Best Manuscript” by the American Heart Association for this work, which highlighted the role of peripheral nerves in cardiac regeneration. Dr. White has lectured and published extensively in the field of stem cell biology, clinical stem cell applications and regenerative medicine. *Connect With Guest* Website: https://neobiosis.com LinkedIn: www.linkedin.com/in/ian-white-phd
Hear how Raina and Marcos supported their 2 HSCs in stopping the daily meltdown cycle, learn how to communicate their emotions safely, and bring back joy in their home, all while homeschooling in a pandemic! If your child is demonstrating the meltdown cycle, watch our free training here: https://www.megghanthompsoncoaching.com/5steps If your teen is struggling to engage with your family, shutting down and struggling with a perfectionism spiral, watch our free training here: https://www.megghanthompsoncoaching.com/5steps_teen If you're ready to book a call with our team, use this link: megghanthompsoncoaching.com/call
What exactly are stem cells, and why are they special? And why are there ethical controversies behind them? Join Maia, Lauren, and Sarah as they discuss the science, medical applications, and bioethics of stem cells. Editor's note CRISPR-Cas9 genetic editing of hematopoietic stem cells (HSCs) to cure the sickle cell anemia mutation is still in early clinical trials - there is no concrete news about long term effects the patients are experiencing. Other stem-cell based strategies (such as approaches to turn on fetal hemoglobin and HSC transplants from healthy donors) have been successful and alleviate patient symptoms! If you are considering a regenerative medicine product and would like more information on how it is regulated, whether it is FDA approved, and more, we encourage you to email ocod@fda.hhs.gov or call 800-835-4709 for more information.
Mushrooms boost immunity, suggests research University of Florida Institute of Food and Agricultural Sciences, April 16, 2022 A new University of Florida study shows increased immunity in people who ate a cooked shiitake mushroom every day for four weeks. In a study led by UF Food Science and Human Nutrition , 52 healthy adults, age 21 to 41, came to the Gainesville campus, where researchers gave them a four-week supply of dry shiitake mushrooms. Participants took the mushrooms home, cleaned and cooked them. Then they ate one, 4-ounce serving of mushrooms each day during the experiment. Through blood tests before and after the experiment, researchers saw better-functioning gamma delta T-cells and reductions in inflammatory proteins. If you eat a shiitake mushroom every day, you could see changes in their immune system that are beneficial. "We're enhancing the immune system, but we're also reducing the inflammation that the immune system produces." (NEXT) Oral milk thistle extract stops colorectal cancer stem cells from growing tumors University of Colorado, April 22, 2022 In results presented at the American Association for Cancer Research, a University of Colorado Cancer Center study shows that orally administering the chemical silibinin, purified from milk thistle, slows the ability of colorectal cancer stem cells to grow the disease. When stem cells from tumors grown in silibinin-fed conditions were re-injected into new models, the cells failed to develop equally aggressive tumors even in the absence of silibinin. "It's very simple: tumors from mice that were initially fed silibinin had fewer cancer stem cells, were smaller, had lower metabolisms and showed decreased growth of new blood vessels. Importantly, when these cancer stem cells from tumors in mice fed silibinin were re-injected into new mice, we found these stem cells had lost their potential to repopulate even in the absence of silibinin exposure. Silibinin is a non-toxic, potentially chemopreventive agent derived from milk thistle seeds. (NEXT) Chilli peppers hold promise of preventing liver damage and progression European Association for the Study of the Liver (Austria), 23 April 2015 Results revealed that the daily consumption of capsaicin, the active compound of chilli peppers, was found to have beneficial effects on liver damage. In the study, capsaicin was found to reduce the activation of hepatic stellate cells (HSCs) in mice models. HSCs are the major cell type involved in liver fibrosis, which is the formation of scar tissue in response to liver damage. The study demonstrates that capsaicin partially improved liver damage in the BDL mice and inhibited further progression of the injury. In the second group of CCl4-treated mice, capsaicin prevented livers from injury development but did not reduce the fibrosis when it was already established. (NEXT) Compassion meditation reduces 'mind-wandering,' research shows Stanford University, April 23, 2022 The practice of compassion meditation may be a powerful antidote to a drifting mind, new Stanford research shows. Compassion meditation focuses on benevolent thoughts toward oneself and others, as the researchers noted. It is different in this aspect than most forms of meditation in the sense that participants are "guided" toward compassionate thoughts. This is the first report that demonstrates that formal compassion training decreases the tendency for the mind to wander, while increasing caring behavior not only towards others but towards oneself. "Mind-wandering" is the experience of having your thoughts not remain on a single topic for long. Prior research suggests that people spend as much as 50 percent of their waking hours in mind-wandering, often without realizing it. Doty said that mindfulness is extremely useful in today's world with its myriad of distractions, as humans are often overwhelmed and can find it difficult to attend to necessary tasks. "By closing one's eyes and engaging in attention training through a mindfulness practice, not only does it diminish the negative physiologic effects of distraction, which can result in anxiety and fear, but it can increase one's ability to attend to important tasks and not have an emotional response to the often negative dialogue which is frequent in many individuals," he said. As the researchers noted, compassion is defined by an awareness of suffering, sympathetic concern and a wish to see the relief of that suffering, and a responsiveness or readiness to help relieve that suffering. The study examined 51 adults during a compassion meditation program, measuring their various states of mind-wandering (neutral, pleasant, and unpleasant topics) and caring behaviors for themselves and others. They were encouraged to meditate at least 15 minutes daily and, if possible, 30 minutes. The results indicated that compassion meditation decreased mind-wandering to neutral topics and increased caring behaviors toward oneself. Videos: Tucker: You are seeing a full-scale attack on free speech How is everything getting coordinated globally on the scale that it is. “Ministry of Truth.”
On this episode of The New Mama Mentor Pod, I am chatting with Melissa Schwartz about how to be a parent that identifies as a Highly Sensitive Person. We dive into the 4 core traits of a highly sensitive person, how to ask for help, and ways to cope with your intense emotions. Melissa Schwartz was born an intense, sensitive, empathic, power seeker. She is a respected expert in the field of Highly Sensitive Children and brings clarity, personal experience and compassion for parents raising HSCs. Her intuitive ability to decode misbehavior and her passion for giving a voice to the legitimate needs of children naturally evolved into becoming the co-creator of Leading Edge Parenting, co-author of Authentic Parenting Power, co-author of Rico's Bumpy Week. She is an internationally acclaimed author, coach and public speaker bringing new perspective based on current research and personal experience to transform the field of child development. Melissa is a Stanford University alumna and a graduate of the University of Massachusetts at Amherst. She lives with her family in Southern California and coaches parents around the world. Resources mentioned in the episode: https://leadingedgeparenting.com https://hsperson.com https://www.audible.com/pd/Quiet-Audiobook/B006TIKUN4?qid=1648186356&sr=1-1&ref=a_search_c3_lProduct_1_1&pf_rd_p=83218cca-c308-412f-bfcf-90198b687a2f&pf_rd_r=1ZGPQGWA3H5J4F59VQAC (Quiet: The Power of Introverts in a World That Can't Stop Talking) Thank you for taking time from your busy day to be here with me another week! Here is my favorite quote from this interview: "If you take the full spectrum of crayons and you color with it, it's going to look brown. Well that's what I call anger. It's usually a handful of emotions that we are coloring the world with." I'd love to hear your thoughts on this episode. You can find me on Instagram at @thenewmamamentor or head to my website contact page and send me a message. The best way to support the show and other moms is to spread the word. If you found this episode helpful and think others would to, feel free to pass it along! Just click the share button! You can also support the show on Patreon at https://www.patreon.com/Thenewmamamentor. Sign up for the $5, $10, or $20 per month levels. You can get great perks like bonus episodes, early access, and The New Mama Mentor merch where a portion of the proceeds is donated to Postpartum Support International to continue supporting new moms' postpartum mental health. And now, the parenting lesson of the week: our expectations of parenthood never match the reality. If you go into parenthood hoping to be the best parent, the only thing you can do to guarantee that is to meet your child where they are physically, emotionally, and developmentally. Until next time! --- Send in a voice message: https://anchor.fm/thenewmamamentor/message Support this podcast: https://anchor.fm/thenewmamamentor/support (https://anchor.fm/thenewmamamentor/support) This podcast uses the following third-party services for analysis: Chartable - https://chartable.com/privacy Podcorn - https://podcorn.com/privacy
In today's episode, we talk about what's worse: surprising your kid with a gift that they hate, but they pretend to love? Or surprising them with a gift you know they LOVE, but suddenly, they hate it? Let me tell you a story. Our family recently adopted a puppy. My kid loves animals and we knew that she wanted a puppy. We knew there would be hyperactivity, lots of licking, jumping around, chewing on stuff, etc. So when we brought it home, I started thinking about how HSCs who are stuck in the meltdown cycle might react to this much sensory overload. Would fear overshadow excitement? Suck up the joy completely before anyone knew what happened? If your child is missing the skills to regulate their emotions and you are walking on eggshells around their explosions, the meltdowns would take the lead here. So, what do you do if your HSC can't handle surprises? Tune in to find out. If your child is demonstrating the meltdown cycle, watch our free training here: https://www.megghanthompsoncoaching.com/5steps If your teen is struggling to engage with your family, shutting down and struggling with a perfectionism spiral, watch our free training here: https://www.megghanthompsoncoaching.com/5steps_teen If you want to learn more about the results we help our clients achieve, visit this page: https://www.megghanthompsoncoaching.com/results To keep up on the latest content, join our Facebook Group: Parent Your Highly Sensitive Child Like a Ninja Be sure to check out our Facebook page: https://www.facebook.com/megghanthompsoncoaching
In today's episode, I interview Carissa, a Bootcamp alumna, that struggled with the 6 meltdowns daily between her two HSCs. Carissa and Jimmy struggled helping their two HSCs manage their big emotions without wanting to check out or yell daily. Carissa felt her family was separating at the seams. She knew, that if she and Jimmy didn't take action on changing the way they parented their two Highly Sensitive Children wouldn't grow up close, nor supportive of each other and that was unacceptable. Learn about the decisions they made to live in a home meltdown free during a pandemic, sustain this lasting change for a year, and feel more empowered than ever...even when the world is saying many people's choices are shrunk. If your child is demonstrating the meltdown cycle, watch our free training here: https://www.megghanthompsoncoaching.com/5steps If your teen is struggling to engage with your family, shutting down and struggling with a perfectionism spiral, watch our free training here: https://www.megghanthompsoncoaching.com/5steps_teen If you want to learn more about the results we help our clients achieve, visit this page: https://www.megghanthompsoncoaching.com/results To keep up on the latest content, join our Facebook Group: Parent Your Highly Sensitive Child Like a Ninja Be sure to check out our Facebook page: https://www.facebook.com/megghanthompsoncoaching
Vor Biopharma's CEO Dr. Robert Ang explains the company's lead engineered hematopoietic cell (eHSC) product candidate, genome engineering of HSCs, and the cell manufacturing process needed to leverage genome engineering technologies for the cells they're creating.
join us as we recap australia's next top model, cycle 7, episodes 12 & 13 including: desert heat, DUBAI, georges antoni, the souk, shit stack, bleached brows and drama HSCs. https://blacklivesmatters.carrd.co/ https://www.the-audacity.com/ https://www.patreon.com/nexttopbestfriend?fbclid=IwAR1YTWcyipBLyEgpj82Ap7f9S5xrRWwZN58Q3ATGQFX3eRbKflizEXQV9wQ nexttopbestfriend@gmail.com https://www.americasnexttopbestfriend.com/ https://www.paypal.me/nexttopbestfriend https://www.instagram.com/nexttopbestfriend/ https://www.facebook.com/nexttopbestfriend/ https://twitter.com/nexttopbestpod amanda: @lochnessmanda (twitter, instagram) hillary: @hillaryous123 (twitter, instagram)
In today's episode, I discuss what could happen if the meltdowns never stop... which sounds terrifying. Tune in to hear how meltdowns play out for HSCs when they grow into adults… and potentially rob them of opportunities. If your child is demonstrating the meltdown cycle, watch our free training here: https://www.megghanthompsoncoaching.com/5steps If your teen is struggling to engage with your family, shutting down and struggling with a perfectionism spiral, watch our free training here:https://www.megghanthompsoncoaching.com/5steps_teen If you want to learn more about the results we help our clients achieve, visit this page:https://www.megghanthompsoncoaching.com/results To keep up on the latest content, join our Facebook Group: Parent Your Highly Sensitive Child Like a Ninja Be sure to check out our Facebook page: https://www.facebook.com/megghanthompsoncoaching
In this episode, I converse with Dr. Rubul Mout, a Research Fellow at Harvard Medical School and at the Stem Cell Program, Boston Children's Hospital (BCH). Born, brought up, and educated in India, Dr. Mout moved to the United States to pursue his PhD from the University of Massachusetts, Amherst. Prior to joining Harvard/BCH, he was a Washington Research Foundation Innovation Fellow at the Institute for Protein Design, University of Washington. Dr. Mout is the inventor of numerous technologies in gene editing and protein design, and in the last decade he developed a technology for intracellular protein delivery and gene editing in mammalian cells, termed as the ‘E-tag' method, which was in 2020, granted a patent by USPTO (the United States Patent and Trademark Office). At Harvard/BCH, Dr. Mout works to engineer bone marrow stem cells (Hematopoietic stem cells, or HSCs) to cure genetic diseases of blood origin and uses various technologies, including techniques he invented in his prior research, to engineer these HSCs. In particular, he uses patient-derived induced pluripotent stem cells (iPSC) to differentiate them to HSC first, and then to produce and train T-cells that could potentially fight off many diseases. We indulge in a terrific conversation on his magnificent journey through science and life inspired by his innate curiosity; growing up in a poor peasant's family in a far remote corner of Northeast India, near the Assam-Arunachal border; undertaking a majestic quest from Tinsukia to Mumbai to Amherst to Seattle to Boston; inspirational mentors like GK Johori, David Baker, Vincent Rotello, and George Daley; fantastic dedication to scientific outreach and activism; writing outstanding books including a popular bestseller that chronicled his splendid journey; and many more things!!
Dr. Jennifer Trowbridge is an Associate Professor at the Jackson Laboratory and an Adjunct Professor at Tufts University. Her lab studies genetics and epigenetics in hematopoietic stem cell biology and leukemia. Their long-term goal is to identify new intervention strategies to extend the “healthspan” of HSCs by maintaining their robust regeneration capacity into older age and reducing risk of development of blood cancers.
In this episode I discuss what a Highly Sensitive person's future and career could look look. Decades of research shows: Sensitive people who grow up in an environment that doesn't fit their emotional needs, which is often due to a mismatch in parenting, develop chronic self harm, aggressive and/or suicidal behaviors. So, what IS possible for your family and HSCs future? Tune in to find out. If your child is demonstrating the meltdown cycle, watch our free training here:https://www.megghanthompsoncoaching.com/5steps If your teen is struggling to engage with your family, shutting down and struggling with a perfectionism spiral, watch our free training here:https://www.megghanthompsoncoaching.com/5steps_teen If you want to learn more about the results we help our clients achieve, visit this page:https://www.megghanthompsoncoaching.com/results To keep up on the latest content, join our Facebook Group: Parent Your Highly Sensitive Child Like a Ninja Be sure to check out our Facebook page: https://www.facebook.com/megghanthompsoncoaching
This episode is about the four main traps that parents of HSCs and Teens fall into when choosing to pick their battles... Because picking your battles is not the solution. It will not support your HSC emotionally. It will not save you energy for the next meltdown. It will not bring your family the peace and joy they deserve. Tune in to learn exactly what I mean by this, and what to do to better serve your family in these situations. If your child is demonstrating the meltdown cycle, watch our free training here:https://www.megghanthompsoncoaching.com/5steps If your teen is struggling to engage with your family, shutting down and struggling with a perfectionism spiral, watch our free training here:https://www.megghanthompsoncoaching.com/5steps_teen If you want to learn more about the results we help our clients achieve, visit this page:https://www.megghanthompsoncoaching.com/results To keep up on the latest content, join our Facebook Group: Parent Your Highly Sensitive Child Like a Ninja Be sure to check out our Facebook page: https://www.facebook.com/megghanthompsoncoaching
Melissa Schwartz was born an intense, sensitive, empathic, power seeker. Her intuitive ability to decode misbehavior and her passion for giving a voice to the legitimate needs of children naturally evolved into becoming the co-creator of Leading Edge Parenting. She is an internationally acclaimed leader in the field of Highly Sensitive Children and brings clarity, personal experience and compassion for parents raising HSCs. She is the co-founder of Leading Edge Parenting, co-author of “Authentic Parenting Power” and author of forthcoming “Under the Hood: A Manual to Understand the Inner Workings of Children”. She is known for helping parents and professionals understand children with high sensitivity and sensory processing disorder. Melissa is a Stanford University alumna and a graduate of the University of Massachusetts at Amherst. She lives in San Diego, California and coaches' parents around the world. And today, she is sharing her wisdom the qualities that highly sensitive people bring to business and entrepreneurship. Today's Mantra/Affirmation: Things are always working out for me. You can find Melissa Schwartz at: https://www.leadingedgeparenting.com https://www.facebook.com/LeadingEdgeParenting https://www.facebook.com/HighlySensitiveChildren https://www.facebook.com/omghsp https://www.facebook.com/groups/highlysensitivechildren https://www.facebook.com/groups/omghsp https://www.instagram.com/leadingedgeparenting https://www.youtube.com/leadingedgeparenting https://www.linkedin.com/in/meschwa/
Despite the remarkable research supporting regenerative medicine like Stem Cells and Exosomes, the FDA has decided to ban the use of these treatments in the US. Many argue that it is because big pharma is looking for a way to monopolies the treatment or effectively end the possibility of it because they have the ability to cure people, instead of keeping them dependent on pills forever, which results in loss revenue for big pharma companies. Besides the obstacles created by the business side of the medical realm, there is a ton of misinformation surrounding the ethics of treatments like Stem Cells. For example, those that are not in the know are under the impression that stem cells are recruited from babies or fetuses that result in pregnancy complications. This couldn't be further from the truth. To get to the bottom of it, I invited Founder, President & Chief Scientific Officer of Neobiosis, Dr. Ian White, onto the show. Enjoy! About Our Guest: Dr. White. is an expert in the field of regenerative medicine and stem cell biology with over 20 years’ experience working in academia and industry. Dr. White received his B.S. from Liverpool John Moores University and his M.S. from the famed Liverpool School of Tropical Medicine in England prior to being hired at Dartmouth College in the United States to study the genetics of gamete biology. In August of 2000 Dr. White was recruited to Harvard University to work with hematopoietic stem cells (HSCs) and immune cell biology under the mentorship of the world-renowned Dr. Laurie Glimcher, where he co-authored several peer-reviewed scientific publications. Dr. White went on to receive his Ph.D. from the Ansary Stem Cell Institute, division of Regenerative Medicine at Cornell University under the guidance of Howard Hughes investigator Dr. Shahin Rafii. During this time Dr. White developed an in vitro method for culture expanding autologous HSCs in an artificial vascular niche for the treatment of cancer patients who have undergone bone marrow ablation following chemo- or radio-therapy. This technology has since gone on to be commercialized by Cornell and the company, Angiocrine, LLC, has become one of the top two HSC expansion companies in the US. Following the completion of his Ph.D., Dr. White spent time as a post-doctoral research scientist in the embryonic stem cell (ESC) laboratory of Dr. Stephen Dalton, whose pioneering work led to the inclusion of c-myc as one of the four factors used by Nobel award winning Dr. Shinya Yamanaka in the generation of induced pluripotent stem cells (iPSCs). Subsequently, Dr. White relocated to the Interdisciplinary Stem Cell Institute at the University of Miami Miller School of Medicine. Dr. White published ground-breaking research in the field of regenerative medicine alongside Dr. Joshua Hare, including a book chapter on the use of Mesenchymal Stem Cells (MSCs) in cardiac regeneration. In 2015, Dr. White’s work on the regeneration of the heart was featured on the cover of Circulation Research, one of the top peer-reviewed journals for cardiovascular medicine in the world. In 2016. Dr. White was honored with an award for the “Best Manuscript” by the American Heart Association for this work, which highlighted the role of peripheral nerves in cardiac regeneration. Dr. White has lectured and published extensively in the field of stem cell biology, clinical stem cell applications and regenerative medicine. To connect with Dr. Ian White, visit: https://neobiosis.com
In this episode of the Epigenetics Podcast, we caught up with Margaret (“Peggy”) Goodell from Baylor College of Medicine in Houston, Texas to talk about her work on the epigenetic regulation of stem cell self-renewal and differentiation. Dr. Margret Goodell's laboratory focuses on how differentiation and self-renewal is regulated in hematopoietic stem cells (HSC). In the early stages of her research career, however, Dr. Goodell was able to develop a new method to purify stem cells. This method was based on the characteristic of stem cells to pump out the Hoechst dye that was used for the purification. In recent years, the focus of the lab has been to identify how HSCs decide whether to self-renew or differentiate. To get an answer to this question, the lab has performed genome-wide screens to find differentially expressed genes during the decision process. By doing that, they recently found that the DNA methyltransferase 3A (DNMT3A) was highly and specifically expressed in HSCs and that it is required for differentiation. When DNMT3A was knocked out in HSCs, the cell population expanded dramatically and the ability to differentiate was impaired. This finding led to further experiments in this area and to the discovery of so-called DNA methylation canyons in the genome, which are large regions of very low DNA methylation that harbor highly conserved regulator genes. In this episode we discuss how Dr. Peggy Goodell described a new approach to isolate hematopoietic stem cells even though she was not looking for that, how she discovered DNMT3A as an important factor in stem cell decision making, and how she entered and approached new fields of research along the path of her research career. References M. A. Goodell, K. Brose, … R. C. Mulligan (1996) Isolation and functional properties of murine hematopoietic stem cells that are replicating in vivo (The Journal of Experimental Medicine) DOI: 10.1084/jem.183.4.1797 Shannon McKinney-Freeman, Margaret A. Goodell (2004) Circulating hematopoietic stem cells do not efficiently home to bone marrow during homeostasis (Experimental Hematology) DOI: 10.1016/j.exphem.2004.06.010 Stuart M. Chambers, Chad A. Shaw, … Margaret A. Goodell (2007) Aging hematopoietic stem cells decline in function and exhibit epigenetic dysregulation (PLoS biology) DOI: 10.1371/journal.pbio.0050201 Grant A. Challen, Deqiang Sun, … Margaret A. Goodell (2011) Dnmt3a is essential for hematopoietic stem cell differentiation (Nature Genetics) DOI: 10.1038/ng.1009 Related Episodes Epigenetic Reprogramming During Mammalian Development (Wolf Reik) Effects of DNA Methylation on Chromatin Structure and Transcription (Dirk Schübeler) CpG Islands, DNA Methylation, and Disease (Sir Adrian Bird) Contact Active Motif on Twitter Epigenetics Podcast on Twitter Active Motif on LinkedIn Active Motif on Facebook Email: podcast@activemotif.com
Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2020.10.28.358705v1?rss=1 Authors: Tepavcevic, V., Dufayet-Chaffaud, G., Aigrot, M.-S., Gillet-Legrand, B., Tada, S., Izagirre, L., Cartier, N., Lubetzki, C. Abstract: Despite extensive progress in immunotherapies that reduce inflammation and relapse rate in patients with multiple sclerosis (MS), preventing disability progression associated with cumulative neuronal/axonal loss remains an unmet therapeutic need. Complementary approaches have established that remyelination prevents degeneration of demyelinated axons. While several pro-remyelinating molecules are undergoing preclinical/early clinical testing, targeting these to disseminated MS plaques is a challenge. In this context, we hypothesized that monocyte (blood) -derived macrophages may be used to efficiently deliver repair-promoting molecules to demyelinating lesions. Here, we used transplantation of genetically-modified hematopoietic stem cells (HSCs) to obtain circulating monocytes that overexpress Semaphorin 3F, a pro-remyelinating molecule. We show that Semaphorin 3F-expressing macrophages quickly infiltrate demyelinating spinal cord lesions, which increases oligodendrocyte progenitor cell recruitment and accelerates myelin repair. Our results provide a proof-of-concept that monocyte-derived macrophages could be used to deliver pro-remyelinating agents "at the right time and place", suggesting novel means for remyelinating therapies in patients with MS. Copy rights belong to original authors. Visit the link for more info
In this episode, Christopher Kennedy, President at Elixell Therapeutics, discusses how his company is committed to working on stem cell expansion and its potentials in healthcare https://outcomesrocket.health/elixell/2020/03/
In this week's episode we will review a study that demonstrates how the impaired hematopoietic stem cells (HSCs) observed in β-thalassemia can be rescued by administration of parathyroid hormone, learn more about genetic variants that can increase the risk of venous thromboembolism, and explore the identification of two DNA methylation subtypes of Waldenström’s macroglobulinemia.
TITLE Flourishing As A Highly Sensitive Person (HSP) During Times of Stress GUEST Alane Freund, MS, MA, LMFT EPISODE OVERVIEW During times of stress, the Highly Sensitive Person can fare better than the other 80% when we cultivate environments that help us flourish, as well as being mindful about what we consume. Overstimulation can look like anxiety, and HSPs can be misdiagnosed with anxiety & depression. Alane talks about optimal levels of stimulation, and she believes that prevention for anxiety is the key. She shares what she does to prevent anxiety. Alane also provides more than 15 resources for HSPs. HIGHLIGHTS For teens and men, we can refer to being an HSP as having a finely tuned nervous system or having a highly reactive brain There are 1.6 Billion Highly Sensitive People Anxiety comes from overstimulation The #1 treatment for anxiety is prevention We need to learn what brings on anxiety We want to find our optimal level of arousal Research studies show that Rhesus monkeys who had higher reactive brains did better when assigned to a Rhesus monkey who was a skilled mother. These monkeys became leaders. The reactive baby monkeys who were assigned to less skilled mothers had higher incidences of sickness and injury Highly Sensitive Children who encounter stressors (marital conflict, shame, etc.) have higher incidences of illness and injury For those 80% of children who don’t have the trait, andare exposed to stress, most had a flat line meaning they were almost not impacted by stressors at all When a Highly Sensitive Child (HSC) has a parent or caregiver who assists the child in managing stressors, those HSCs have better outcomes and are much less likely to have problems compared to children without the trait exposed to the same stressors Those HSCs that have many stressors (or high stress) in their environments are more likely to experience illness or injuries, including adult anxiety or depression. However, HSPs and HSCs are more responsive to intervention and treatment than the 80% who are not sensitive Many HSCs tend to be more articulate, have a keen sense of humor, they get along well with adults (mini-adults) They can also be late bloomers, and slow developers due to DOES DOES—4 Core Characteristics Dr. Elaine Aron Identified D—depth of processing O—Overarousal or Overstimulation E—Emotional Responsiveness/Empathy S—Sensitive to Subtle Stimuli It’s not uncommon for HSPs to be diagnosed with depression & anxiety. To the outside observer, what is depth of processing and being thoughtful or becoming overaroused may be misinterpreted as depression and/or anxiety Children need an adult who gets them and understands them (not a parent) Every little bit of good stuff we got in childhood, we use moving forward During challenging times, HSPs fare better. We process and we know what’s needed We need to help young girls feel better about themselves We provide emotional leadership in our families and communities. Our species needs this to thrive We need to find ways to discharge what we are processing Journaling can be very helpful Extroverts may choose to process verbally Overstimulation looks like anxiety, which can become a habit. On the continuum is stress on one end and panic attacks on the other end What can we do to prevent the climb up the scale Prevention for anxiety is the key Who knows what it is for you—you know the truth; you see the truth Suggestions: Meditate 2x/day Meditation is not optional for HSPs We need to revisit spirituality Exercise (until you sweat) daily Take a sip of water every 15-20 minutes Close your eyes to reduce overstimulation and to reset (80% of stimulation comes in visually) Boredom is understimulation—you need to do something to alleviate the boredom It’s important to find the optimal level of stimulation (not over or under stimulation)—good luck with this one! Alane says to call her if you need help figuring this out. If you think you’re an HSP and take the adult version of the test, but don’t score in the range, take the child’s version of the test and think back to when you were a child and what you remember hearing about yourself When it’s hard; it’s hard--lean into the hard and let it be until you can remember to do self-care We need to read, especially fiction with a good plot as another way to rest our minds Alane suggests audiobooks because they can be soothing without getting overstimulated. A few of her suggestions are listed under resources HSPs do best when they participate in good therapy or coaching Our planet is healing with this pandemic Opposite Reframe: The world is upside down right now, so “What is right side up?” The importance of breath Bellows breath Pranyama Breathing/Alternate nostril breathing Practice doing a hard style plank where you tense your core for 5-10 seconds, which can interrupt anxiety (and you can do this sitting in your chair or standing as well) Practice a Loving Kindness Meditation When we pray or meditate for others it eases our anxiety HSPs can find it difficult to pray for or focus on ourselves, so start with loving kindness for others. Our empathy makes this easier. Codependency is NOT the same as empathy We can have loving detachment and care for others Your caring for others does not have to be at the risk or detriment of caring for yourself It’s important that we take care of ourselves—we are the vessel We are the emotional leaders We need to protect the sensitive children We can stop others from shaming them for having feelings; they are the ones who will change the world GUEST BIO Alane Freund, MS, MA, LMFT, (Lic. #36077) has helped adults, youth, and families focus on solutions through psychotherapy and consultation over three decades in the mental health field. An International Consultant on High Sensitivity (ICHS) working closely with Dr. Elaine Aron, Ms. Freund has developed and implemented programs for highly sensitive people, children, families, and clinicians who serve them. She also holds Masters’ degrees in clinical psychology and school counseling. Ms. Freund specializes in family therapy and education with and about highly sensitive people and LGBTQ+ families. A skilled facilitator and therapist, an HSP herself, and the parent of a highly sensitive young adult, she teaches workshops, offers consultation, has a twice monthly Are You Highly Sensitive LIVE Q&A webinar, and leads groups and retreats, including the HSPs & Horses™ retreats at Heart and Mind Equine in Northern California with Elaine Aron, PhD (CEs offered). Ms. Freund is an instructor at retreat centers, Kripalu in Massachusetts and 1440 Multiversity in California, as well as teaching at the California Institute for Psychotherapy and the California Institute of Integral Studies in California. She can be reached for consultation or referrals through her website, alanefreund.com. PODCAST HOST Patricia knows what it’s like to feel like an outcast, misfit, truthteller, and black sheep. Learning about the trait of being a Highly Sensitive Person (HSP), helped Patricia rewrite her history with a deeper understanding, appreciation and compassion. She created the podcast Unapologetically Sensitive to help other HSPs know that they aren’t alone, and that being an HSP has amazing gifts, and some challenges. Patricia works online globally working individually with people, and she teaches Online Courses for HSPs that focus on self-care, self-compassion, boundaries, perfectionism, mindfulness, and creating a lifestyle that honors us LINKS Alane’s Links This is a live (on Zoom) panel I led with some other members of the International Consultants on High Sensitivity (Elaine Aron's hand-selected and trained consultants.) There are even more videos, so you can subscribe to Alane Freund LMFT on You Tube to see more. Are You Highly Sensitive LIVE…a twice a month live webinar for Q&A with highly sensitive people and parents raising highly sensitive children. For every webinar, members get to ask questions (ahead of time or live), and I do my best to answer them! We are building a really lovely community at areyouhighlysensitive.com. Listeners of the podcast will get 50% off their first month if you type in the discount code: OFFERUS Website for therapy, speaking, consultation: alanefreund.com Facebook Alane Freund Alane Freund LMFT Understanding the Highly Sensitive Child and Teen Heart and Mind Equine I am an admin of Elaine Aron and High Sensitivity Facebook group which I invite you all to join. I recommend you say “referred by Alane Freund in the questions to join so you are accepted more readily. Instagram @alanefreundlmft Bellows breath-- https://chopra.com/articles/how-and-why-to-perform-bhastrika-breath 10 minute Yoga with Adrienne-- https://www.google.com/search?q=10+minute+yoga+with+adrienne&rlz=1C1CHBF_enUS843US843&oq=10+minute+yoga+with+adrienne&aqs=chrome..69i57j0l5.6818j0j9&sourceid=chrome&ie=UTF-8 Sun Salutation Yoga with Adrienne--https://www.google.com/search?q=yoga+with+adrienne+sun+salutation&rlz=1C1CHBF_enUS843US843&oq=yoga+with+adrienne+sun+salutation&aqs=chrome..69i57j0l4.4621j0j7&sourceid=chrome&ie=UTF-8 Pranyama Breathing-- https://www.youtube.com/watch?v=395ZloN4Rr8 Hard Style Plank Tense Core-- https://www.youtube.com/watch?v=4jTOit8PkVY Loving Kindness Meditation-- https://self-compassion.org/guided-self-compassion-meditations-mp3-2/ Movie—Sensitive: The Untold Story Dr. Elaine Aron’s website--https://hsperson.com/ Pema Chodrin-- https://pemachodronfoundation.org/product-category/products/ Linda Graham—Bouncing Back-- https://lindagraham-mft.net/ Eve Decker Blog—Loving Kindness https://evedecker.com/lovingkindness/ Ted Zeff—Strong Sensitive Boy http://drtedzeff.com/ Chief Inspector Gamache Louise Penny Series-- https://www.gamacheseries.com/about/ Harry Potter series-- https://www.wizardingworld.com/discover/books Outlander—series http://www.dianagabaldon.com/books/outlander-series/ Patricia’s Links HSP Online Course--https://unapologeticallysensitive.com/hsp-online-groups/ To write a review in itunes: click on this link https://itunes.apple.com/us/podcast/unapologetically-sensitive/id1440433481?mt=2 select “listen on Apple Podcasts” chose “open in itunes” choose “ratings and reviews” click to rate the number of starts click “write a review” Website--www.unapologeticallysensitive.com Facebook-- https://www.facebook.com/Unapologetically-Sensitive-2296688923985657/ Closed/Private Facebook group Unapologetically Sensitive-- https://www.facebook.com/groups/2099705880047619/ Closed/Private Facebook group for Therapists and Healers--https://www.facebook.com/groups/208565440423641/ Instagram-- https://www.instagram.com/unapologeticallysensitive/ Youtube-- https://www.youtube.com/channel/UCOE6fodj7RBdO3Iw0NrAllg/videos?view_as=subscriber Meetup-- https://www.meetup.com/Unapologetically-Sensitive-Meetup/ e-mail-- unapologeticallysensitive@gmail.com Show hashtag--#unapologeticallysensitive Music-- Gravel Dance by Andy Robinson www.andyrobinson.com
In this episode, Christopher Kennedy, President at Elixell Therapeutics, discusses how his company is committed to working on stem cell expansion and its potentials in healthcare https://outcomesrocket.health/elixell/2020/03/
Host Kevin Patton emphasizes the idea of the "last best story" in science to review the unfolding debates about adult neurogenesis and autonomic pathways. How can we use the "anatomical compass" to help students learn anatomy? What is reserve hematopoiesis? And more discussion of feedback to students in online tests. 01:17 | Feedback in Online Tests 08:17 | The Anatomical Compass 14:47 | Sponsored by AAA 15:12 | Reserve hematopoiesis 18:09 | Sponsored by HAPS 18:54 | Featured: Last Best Story in Adult Neurogenesis & ANS Pathways If you cannot see or activate the audio player click here. Questions & Feedback: 1-833-LION-DEN (1-833-546-6336)Follow The A&P Professor on Twitter, Facebook, Blogger, Nuzzel, Tumblr, or Instagram! Scientific theories are tested every time someone makes an observation or conducts an experiment, so it is misleading to think of science as an edifice, built on foundations. Rather, scientific knowledge is more like a web. The difference couldn't be more crucial. A tall edifice can collapse – if the foundations upon which it was built turn out to be shaky. But a web can be torn in several parts without causing the collapse of the whole. The damaged threads can be patiently replaced and re-connected with the rest – and the whole web can become stronger, and more intricate. (Massimo Pigliucci) 1 | Feedback in Online tests 7 minutes In Episode 36, Adam Rich called in regarding how we can provide feedback to students taking online tests. I responded that I encourage students to get the correct response from their study buddies—or from me. After the episode aired, Krista Rompolski pointed out that this could be a challenge in very large courses. What do y'all think? Tell us. Really. Big Year in Anatomy & Physiology Teaching with The A&P Professor | Episode 36 Spaced Retrieval Practice | Episode 1 Test Debriefing Boosts Student Learning | Episode 11 Test Frequency in the A&P Course | Episode 33 Long Term Learning | Five Strategies for Teaching A&P Testing as Teaching Here's a tool I use for repeated feedback (saves a LOT of time): Sign up for TextExpander. Recall your best words. Instantly, repeatedly. We want your feedback! 1-833-LION-DEN or 1-833-546-6336 podcast@theAPprofessor.org @theAPprofessor (Facebook, Twitter, Instagram, Pinterest, and beyond) 2 | The Anatomical Compass 6.5 minutes Although you and I are comfortable in orienting ourselves to anatomical directions when looking at diagrams, photographs, and specimens in anatomy, our beginning student often are not. The simple process of adding an "anatomical rosette" reflecting the anatomical directions in each encountered diagram can help students develop the skill of understanding anatomical perspective. Anatomical Rosettes Help Student Orient Themselves 3 | Sponsored by AAA 0.5 minutes The searchable transcript for this episode, as well as the captioned audiogram of this episode, are sponsored by The American Association of Anatomists (AAA) at anatomy.org. Their big meeting is in April at the Experimental Biology (EB) meeting in Orlando FL. Check it out! Searchable transcript Captioned audiogram 4 | Reserve Hematopoiesis 3 minutes Hematopoietic stem cells (HSCs) may have a "back-up system" that helps out after damage to the working population. These "reserve" HSCs (rHSCs) may step up when the primed HSCs (pHSCs) cannot keep up with the demand for hematopoiesis. Scientists have identified a bone marrow backup system (summary article) my-ap.us/2BmcoE0 N-Cadherin-Expressing Bone and Marrow Stromal Progenitor Cells Maintain Reserve Hematopoietic Stem Cells (report by Zhao, et al. in Cell Reports) my-ap.us/2Bk7vLN 5 | Sponsored by HAPS 0.5 minutes The Human Anatomy & Physiology Society (HAPS) is a sponsor of this podcast. Did you know there's a one-day regional HAPS conference in March? Check it out. You can help appreciate their support by clicking the link below and checking out the many resources and benefits found there. Anatomy & Physiology Society theAPprofessor.org/haps 6 | Featured: Last Best Story in Adult Neurogenesis & ANS Pathways 12 minutes The "last best story" is what I tell my students I'm providing to them. That approach emphasizes the evolving nature of scientific understanding. In this episode, I mention two stories that are evolving right now. Storytelling is the Heart of Teaching A&P | Episode 12 (where I introduce the idea of teaching as storytelling) Adult neurogenesis in the brain Running Concept Lists Help Students Make Connections | Episode 8 (where I first discuss this story) The Discovery of the Neuron (outlines the origin of central dogmas about neuroscience, including Ramón y Cajal's role) Neurogenesis in the adult human hippocampus (paper that established the idea that adult brain neurogenesis does occur) Human hippocampal neurogenesis drops sharply in children to undetectable levels in adults (paper that challenges the idea of adult brain neurogenesis) New Study Questions Confidence in Neurogenesis in the Adult Brain (article that summarizes the recent controversy) Are Learning Styles Real? Why or Why Not? | Episode 14 (where I bring up newer research on adult neurogenesis) New Evidence Suggests Aging Brains Continue to Make New Neurons (article by Francis Collins on the new paper) Human Hippocampal Neurogenesis Persists throughout Aging (new research paper in Cell) The last best story in adult neurogenesis? A New Look at Neurogenesis in Humans (blog post by Neuroskeptic, summarizing new perspectives) my-ap.us/2TDxTXU Recalibrating the Relevance of Adult Neurogenesis (article by Jason S. Snyder in Trends in Neurosciences) my-ap.us/2TEb5r4 Are sacral autonomic pathways sympathetic or parasympathetic? Sacral Efferent Pathways are Sympathetic, Not Parasympathetic (summary from The A&P Professor blog) my-ap.us/2TJMHnS The sacral autonomic outflow is sympathetic (I. Espinosa-Medina, O., et al., of J.-F. Brunet lab's in Science the proposed change; includes an updated version of the classic diagram of sympathetic and parasympathetic pathways) my-ap.us/2fNdcF3 Neural circuitry gets rewired (Adameyko, I. in Science comments on the report cited above, stating that "This finding provokes a serious shift in textbook knowledge, and, as with any fundamental discovery, it brings important practical implications..." and goes on to mention of a few of the implications (e.g., how to treat bladder dysfunction) my-ap.us/2gg9O8P The Autonomic Nervous System. Part I. (John Newport Langley's classic "primary source" that codified the modern concept of the ANS.) my-ap.us/2fYHt3M The sacral autonomic outflow is parasympathetic: Langley got it right (John P. Horn's commentary in Clinical Autonomic Research; the last best story?) my-ap.us/2TCvwF5 If the hyperlinks here are not active, go to TAPPradio.org to find the episode page. More details at the episode page. Transcript available at the script page. Listen to any episode on your Alexa device. Join The A&P Professor social network: Blog Twitter @theAPprofessor Facebook theAPprofessor Instagram theAPprofessor YouTube Amazon and TextExpander referrals help defray podcasting expenses.Transcript and captions for this episodeare supported by theAmerican Association of Anatomists.anatomy.org The Human Anatomy & Physiology Societyalso provides support for this podcast. theAPprofessor.org/haps(Clicking on sponsor links helps let them know you appreciatetheir support of this podcast!)
EPISODE Episode 13 TITLE Parent Your Highly Sensitive Child Like a Ninja GUEST Megghan Thompson, LCPC EPISODE OVERVIEW Parenting a Highly Sensitive Child (HSC) can be challenging—especially if your child has meltdowns. Megghan talks about the Shame Meltdown Cycle; What works to raise an HSC; how to avoid sending the message to your child that that they are wrong; how to communicate with your HSC to strengthen your relationship, and effective ways to communicate with your child to build a solid relationship and mirror your child’s strengths. We talk about the challenges that external validation (reward/sticker programs) pose as well as ways to avoid communicating in a way that increases shame and perfectionism. highlights DBT—Dialetctical Behavioral Therapy Vantage Sensitivity—when parents change how they parent, the Highly Sensitive Child (HSC) will benefit more than a non-HSC, and the HSC will feel better about themselves. The HSC will thrive and do much better and will respond faster than a non HSC. Differential Susceptibility—Highly Sensitive Persons (HSPs) suffer more in highly negative environments compared to non HSPs. The opposite is also true in the HSPs benefit more in highly positive environments than non HSPs. HSPs/HSCs also notice more positive things in positive environments A little bit of positivity can give an HSP hope, but if an HSC is having frequent meltdowns, they end up feeling incapable and say things like, “You guys would be better off without me.” Or “I wish I was in a different family.” “I wish I were dead.” For children, a little bit of positivity goes a long way Traditional parenting says it’s the child that needs to learn skills, but Megghan has learned that when the family changes the environment and how they parent, everyone benefits The HSC changes how he/she feels about himself/herself when the parents change the environment Shame can be pervasive for HSCs and HSPs Shame Meltdown Cycle— The HSC feels shame—I’m a bad kid; I didn’t do it fast enough or good enough This is followed by having big feelings—they are told not to worry They learn to hide their emotions, internalize, or think there is something wrong with them What does the HSC do with these feelings—they learn to stuff, bottle or deny their feelings Eventually the child explodes They are given a consequence, but there is no repair work; no tools taught, the family is still not talking about feelings or validating the HSC’s feelings so the cycle repeats itself Don’t tell your child not to worry! It’s really important to validate their feelings! BIO Megghan Thompson is a Licensed Clinical Professional Counselor, Registered Play Therapist-Supervisor and Parent Coach who has specialized in working with Highly Sensitive Children and their parents for a decade. She helps parents eliminate daily meltdowns in as little as 8 weeks without giving in or doing a complete overhaul of their household, and strengthen the relationship with their child in the process. Growing up with a Highly Sensitive sister, she also has firsthand experience of the struggles families face when they don't understand the needs of Highly Sensitive Children. She has extensive training and experience working with sensitive teens who engage in self-harmful and life-threatening behaviors, and has made it her mission to build an army of ninja parents of Highly Sensitive Children to prevent this from happening for other sensitive kids and their families. She owns a group practice in Mount Airy, Maryland that specializes in treating Highly Sensitive Children, teens, and their families. You can find her on Facebook through her group: Parent Your Highly Sensitive Child Like a Ninja, and a link to her free training for parents of HSCs will be in the show notes. LINKS Parent Your Highly Sensitive Child Like a Ninja Facebook Group: https://goo.gl/yTre1h Free Parent Training: https://www.megghanthompsoncoaching.com/p/5steps Schedule a Free Consultation: https://www.megghanthompsoncoaching.com/p/talk Dr. Elaine Aron’s website— https://hsperson.com/ HSP Self-test-- https://hsperson.com/test/highly-sensitive-test/ HSP Child self-test-- https://hsperson.com/test/highly-sensitive-child-test/ Introversion, Extroversion and the Highly Sensitive Person by Jaquelyn Strickland, LPC-- https://hsperson.com/introversion-extroversion-and-the-highly-sensitive-person/ To write a review in itunes: click on this link https://itunes.apple.com/us/podcast/unapologetically-sensitive/id1440433481?mt=2 select “view in itunes” chose “open in itunes” choose “ratings and reviews” click to rate the number of starts click “write a review” Website--www.patriciayounglcsw.com Podcast--www.unapologeticallysensitive.com Facebook--https://www.facebook.com/Patricia-Young-LCSW-162005091044090/ Facebook group Unapologetically Sensitive-- https://www.facebook.com/groups/2099705880047619/ Instagram--https://www.instagram.com/unapologeticallysensitive/ Youtube--https://www.youtube.com/channel/UCOE6fodj7RBdO3Iw0NrAllg/videos?view_as=subscriber e-mail—unapologeticallysensitive@gmail.com Show hashtag--#unapologeticallysensitive MUSIC—Gravel Dance by Andy Robinson www.andyrobinson.com Youtube--https://www.youtube.com/channel/UCOE6fodj7RBdO3Iw0NrAllg/videos?view_as=subscriber e-mail--info@unapologeticallysensitive.com Show hashtag--#unapologeticallysensitive MUSIC—Gravel Dance by Andy Robinson www.andyrobinson.com
You're listening to the July edition of 3 Minute 3Rs, brought to you this month by the North American 3Rs Collaborative (www.na3rsc.org), Lab Animal (www.nature.com/laban) and the NC3Rs (www.nc3rs.org.uk). This month, we'll be talking about refining mouse anesthesia, replacing mice with zebrafish for studying human hematopoetic stem cells, and validating genome-edited mouse lines. The papers behind the pod: * Effects of Ketamine Compared with Urethane Anesthesia on Vestibular Sensory Evoked Potentials and Systemic Physiology in Mice: http://www.ingentaconnect.com/content/aalas/jaalas/2018/00000057/00000003/art00007;jsessionid=2je0i010idu59.x-ic-live-03 * A method for transplantation of human HSCs into zebrafish, to replace humanised murine transplantation models :https://f1000research.com/articles/7-594/v1 * Application of long single-stranded DNA donors in genome editing: generation and validation of mouse mutants: https://bmcbiol.biomedcentral.com/articles/10.1186/s12915-018-0530-7 See acast.com/privacy for privacy and opt-out information.
Does your child sometimes feel like a ticking time bomb? Ready to explode at any moment? That's how it was for me with my oldest daughter. It seemed like almost everything caused her some kind of distress. She was SO attuned to my moods; we had to cut out all of the tags in her clothes; and if she missed a nap? Forget it, we were doomed. There was SO much to learn. There were so many skills I didn't have! I later realized that Maggie is a Highly Sensitive Child. And I realized that someone else was Highly Sensitive too (can you guess who that is?). On this episode, I talk to Melissa Schwartz, expert on Highly Sensitive Children and how to take care of them. What is your takeaway? Click here to start the conversation! Melissa is a respected expert in the field of Highly Sensitive Children and brings clarity, personal experience and compassion for parents raising HSCs so they can thrive in a de-sensitized world. Find out more about Melissa's work at Leading Edge Parenting. Fan of the Mindful Mama Podcast? Support it by leaving a quick review -----> on iTunes or on Stitcher (or wherever you listen!) ABOUT HUNTER CLARKE-FIELDS: Hunter Clarke-Fields is a mindfulness mama mentor. She coaches overstressed moms on how to cultivate mindfulness in their daily lives. Hunter is a certified Parent Effectiveness Training teacher and has over 20 years of experience in yoga & mindfulness practices. She has taught thousands worldwide. Hunter is the creator of the Mindful Parenting course. Download the audio training, Mindfulness For Moms (The Superpower You Need) for free! It's at mindfulmomguide.com. Find more podcasts, blog posts, free resources, and how to work with Hunter at HunterClarkeFields.com.
"Some highly sensitive children are fairly difficult–active, emotionally intense, demanding, and persistent–while others are calm, turned inward, and almost too easy to raise except when they are expected to join a group of children they do not know. But outspoken and fussy or reserved and obedient, all HSCs are sensitive to their emotional and physical environment” -Dr. Elaine Aron. If you are a parent of an HSC, or you were an HSC, you are well aware of the gifts and challenges associated with the trait of sensitivity. On this episode of HSP SOS, Michelle and The Captain talk with fellow HSP Melissa Schwartz. Melissa is a coach for parents of highly sensitive children, author, speaker, and co-creator of Leading Edge Parenting, not to mention just an enthusiastic advocate for HSPs internationally. It’s HSP SOS #27 Highly Sensitive Children! ----- Website www.leadingedgeparenting.com Facebook Pages/ Groups fb.com/leadingedgeparenting fb.com/highlysensitivechildren fb.com/groups/highlysensitivechildren Authentic Parenting Power by Sandi Schwartz (Author) & Melissa Schwartz (Contributor) http://www.amazon.com/dp/1492168246 ----- ---Subscribe to the HSP SOS Podcast!--- iTunes https://itunes.apple.com/us/podcast/hsp-sos/id1221116848 Google Play https://play.google.com/music/listen#/ps/Inavjhxeg2rdkub26frsppa7pca ---Follow HSP S.O.S. on social media--- On Facebook at: https://www.facebook.com/hspsos On Twitter at: @hsp_sos ---More episodes, articles, and resources!--- Website: http://hspsos.com/ Libsyn Podcast Page: http://hspsos.libsyn.com/ ---Contact Michelle Lynn with questions, comments, or show ideas!--- feelzspecialist@gmail.com ---Contact The Captain (Brian) with questions, comments, or show ideas--- cap@thecaptainspod.com Visit http://thecaptainspod.libsyn.com/ for other related podcasts! ---- Hashtags: #HighlySensitivePersons #HSP #HighlySensitiveChildren #HSC #ElaineAron #Introverts #Parenting Keywords: Highly Sensitive Persons, HSP, Highly Sensitive Children, HSC, Elaine Aron, introverts, parenting
Practical Research Parenting Podcast| evidence-based | raising children | positive parenting
Show Notes: How to Help your Sensitive Child to Thrive This is the second episode on understanding and raising a highly sensitive child (The first episode in the series is here). We get some great tips on helping our children to function socially in a society that values being tough, learn more about Equine Assisted Learning, and discuss mindfulness and counseling as a highly sensitive person. Summary For the foundations, check out the first episode on Highly Sensitive Children. How can Highly Sensitive Children (HSC) thrive in societies that value being tough? Common phrases in these societies might be "When the going gets tough, the tough get going", "You're too sensitive", and "I'll give you something to cry about". Parent and child need to understand that highly sensitive people process the world differently. It is appropriate for HSCs to hold back, and take time to process before they are required to respond and react. Visit the new school before the first day. Minimise the number of new experiences experienced at one time. Rhesis monkeys have society and parenting styles very similar to humans. Researchers allocated "uptight" (Highly Sensitive) infant monkeys to responsive or unresponsive parents. "Uptight" infants with skilled, responsive parents thrived and became the leaders of the group. "Uptight" infants with unresponsive parents they were the lowest functioning in their group. (1) Should parents be helping HSCs to "harden up"? HSCs have differential susceptibility. That means negative and positive experiences affect them more than non-HSCs. Everything we do to help support HSCs to have positive experiences have great effect. In contrast forcing scary/noisy/overstimulating experiences on HSCs can make things a lot worse. Teaching, learning, and modelling self-care is really important. Avoiding hunger, getting good sleep, scheduling in time out etc. With a childhood that honors sensitivity, HSCs can grow into really amazing adults. Equine-assisted learning Equine assisted Growth And Learning Association (EGALA) model. Alane Freund met Elaine Aron, the original researcher on high sensitivity, through horse riding. EGALA is ideal for HSPs because they tend to do well in nature and love animals. Alane and Elaine developed workshops: "HSPs and Horses". Alane became certified as therapist for HSPs in addition to Marriage and Family Therapist and EGALA training. Workshops are comfortable, they encourage people to take breaks and snack throughout. Alane Freund Has been horse training and riding since childhood. Her high empathy for others led naturally into counselling. Has been a Marriage and Family Therapist for about 25 years. Counselling as an HSP Can be very confronting. Alane found it helps to envisage a beautiful box inside her during client sessions. She opens the box to receive the client's experience and thoroughly explore it during the session. Then when the session is finished, she closes the box. When seeing clients with an HSP's level of depth, we cannot accept the same level of case load. Mindfulness Can help, but can also be problematic because HSCs are deep thinkers, they can get lost in their own thinking. Focusing on nature can really help. Links Patreon Support Page at https://www.patreon.com/PracticalResearchParenting where you can help me to continue these podcasts for you and millions of others. Self test for adults and children at hsperson.com Books on highly sensitive people and children at hsperson.com/store/bookstore/ Recommend Elaine Aron's book "The Highly Sensitive Child" and Ted Zeff's book "Strong sensitive boy". Blog post where I discovered the trait: 7 Things You Should Never Say to the Parent of a Highly Sensitive Child (by Megan Stonelake) References 1. Suomi, S. J. (1991). Uptight and laid-back monkeys: individual differences in the res...
Interview with Kateri Moore, from USA. Kateri Moore discusses the topic 'Hematopoietic stem cell HSCs Of Mice and Men'.The interview is led by Shaun McCann, Chair of EHATol Unit, Member of EHA Education Committee.
Interview with Kateri Moore, from USA. Kateri Moore discusses the topic 'Hematopoietic stem cell HSCs Of Mice and Men'.The interview is led by Shaun McCann, Chair of EHATol Unit, Member of EHA Education Committee.
Liver repair involves phenotypic changes in hepatic stellate cells (HSCs) and reactivation of morphogenic signaling pathways that modulate epithelial-to-mesenchymal/mesenchymal-to-epithelial transitions, such as Notch and Hedgehog (Hh). Hh stimulates HSCs to become myofibroblasts (MFs). Recent lineage tracing studies in adult mice with injured livers showed that some MFs became multipotent progenitors to regenerate hepatocytes, cholangiocytes, and HSCs. We studied primary HSC cultures and two different animal models of fibrosis to evaluate the hypothesis that activating the Notch pathway in HSCs stimulates them to become (and remain) MFs through a mechanism that involves an epithelial-to-mesenchymal–like transition and requires cross-talk with the canonical Hh pathway. We found that when cultured HSCs transitioned into MFs, they activated Hh signaling, underwent an epithelial-to-mesenchymal–like transition, and increased Notch signaling. Blocking Notch signaling in MFs/HSCs suppressed Hh activity and caused a mesenchymal-to-epithelial–like transition. Inhibiting the Hh pathway suppressed Notch signaling and also induced a mesenchymal-to-epithelial–like transition. Manipulating Hh and Notch signaling in a mouse multipotent progenitor cell line evoked similar responses. In mice, liver injury increased Notch activity in MFs and Hh-responsive MF progeny (i.e., HSCs and ductular cells). Conditionally disrupting Hh signaling in MFs of bile-duct–ligated mice inhibited Notch signaling and blocked accumulation of both MF and ductular cells. Conclusions: The Notch and Hedgehog pathways interact to control the fate of key cell types involved in adult liver repair by modulating epithelial-to-mesenchymal–like/mesenchymal-to-epithelial–like transitions.
There's lots of opportunity to light a fire under your marketing efforts for the rest of the year as you plan for 2010.
Medizinische Fakultät - Digitale Hochschulschriften der LMU - Teil 05/19
The hallmark of hematopoietic stem cells (HSC) is their ability of self-renewal and differentiation into multiple hematopoietic cell lineages. Although the molecular network controlling stem cell fate decisions is largely unknown, multiple studies have attributed a key role to transcription factors in this developmental process. In this context the family of homeobox genes was characterized as ‘master genes’ of this early hematopoietic development. The identification of new genes involved in normal and leukemic hematopoiesis and the development of therapies against deregulated processes in hematopoiesis are the major goals in experimental and clinical hematology. . The identification of new genes involved in normal and leukemic hematopoiesis and the development of therapies against deregulated processes in hematopoiesis are the major goals in experimental and clinical hematology. Therefore, the focus of this thesis was the characterization of two novel putative regulatory proteins of early human hematopoiesis, the hematopoietic PBX-interacting protein (HPIP) and the human Vent-like Homeobox gene (VENTX2) and to investigate to the activity of the FLT3 protein kinase inhibitor SU5614 on leukemic blast from AML patient samples. Using complex in vitro assays we analyzed the impact of constitutive expression of HPIP and VENTX2 on stem cell and early human hematopoietic development. To detect clonal progenitor cells primary and secondary colony-forming-unit (CFC) assays were performed. In addition the in vitro equivalent of HSC long-term culture initiating cells were detected with the (LTC-IC) assay. We were able to show that the constitutive expression of HPIP can rapidly lead to increased numbers of cells detected on the level of committed clonogenic progenitor cells and LTC-ICs. In addition, the production of CFC per LTC-IC is markedly enhanced when cord blood (CB) cells are transduced with HPIP as compared to the control. Notably, besides its effect on maintenance of primitive hematopoietic progenitor cells, constitutive expression of HPIP did not block terminal hematopoietic differentiation. Additional we could show that the constitutive expression of HPIP leads to an increase of myeloid cells in transplanted NOD/SCID mice. These data characterize HPIP as a novel regulator of the early human hematopoietic stem cell, demonstrating that its constitutive expression has a notable impact on self renewal and differentiation of human hematopoietic stem cells. In vitro and in vivo analyses shed light on the understanding to the function of the homeobox gene VENTX2. On the level of the most primitive hematopoietic progenitors we could not observe a significant increase in the frequency of HSCs. Furthermore, the number of colonies generated per LTC-IC did not significantly differ between the VENTX2 arm and the control arm. A strong effect was obtained on the level of clonogenic progenitor cells. VENTX2 increased the production of myeloid cells 1.7-fold in comparison to the control. Secondary replating assay confirmed the amplificatory effect of VENTX2 transduced cells in the number of secondary G-CFU indicating that VENTX2 promote myeloid lineage differentiation. Interestingly, on the level of clonogenic progenitors VENTX2 expression resulted in a significantly decreased growth of erythroid colonies by 4.2-fold compared to the control suggesting that constitutive expression of VENTX2 may inhibit early erythroid differentiation. This inhibition did not occur on the level of primitive hematopoietic cells detected by Limiting Dilution LTC-IC assay where VENTX2 increased within a 2.2 fold compared to the control. The observation that VENTX2 overexpression drives CD34+ to differentiate into myeloid lineage was additional proved by in vivo experiments. In NOD/SCID mice VENTX2 induced a 3-fold increase in the proportion of CD15+ mature myeloid cells within the GFP-positive compartment compared to the control. A 7-fold increase was observed in the total of CD38+ GFP+ cells in comparison to the MIG mice control (p
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