POPULARITY
Gaining clarity when you have doubts, fears or conflicting parts of yourself through integration work and shadow work. More from me at KirstyDee.com and IG @KirstyDeeSharesText the show. Ask a question
Weihnachten, Ramadan, Chanukka: Nur eines dieser Feste wird in NRW auch gesetzlich gefeiert. Doch unser Land ist geprägt durch verschiedene Religionen und Kulturen. Welchen Platz haben diese in Ihrem Leben? Wie bereichert Sie das? Diskussion mit Prof. Dr. Gundula Gwenn Hiller und Moderator Jürgen Wiebicke. Von WDR 5.
Integrating both male and feminine energies is beneficial for multidimensional beings. Embracing the totality of the self also aligns with Unconditional Love for the self, which adds to head-and-heart coherence. This coherence creates ISREAL within. Oneness is the pure awareness that everything flows through us and we are everything there is. Absolutely nothing exists outside of consciousness we are the Divine Mind itself.
In this episode, Oluwakayode and Charles Aigbona delve into the crucial role of family in nation building. Join them as they explore how family structures, values, and dynamics contribute to the development and progress of a nation.
TruthSeekah and Daniel had a wide-ranging conversation about spirituality, psychedelics, and personal experiences. They discussed the definition of a shaman and the importance of new terminology to describe modern spiritual experiences. They also shared their experiences with plant medicines and the importance of treating these substances as sacred tools for healing and growth. The conversation covered a variety of spiritual practices, including cow cakow ceremonies and ayahuasca ceremonies. They also discussed the interplay between nature and human experience, the concept of the afterlife, and the importance of gratitude and appreciation. Daniel emphasized the importance of physical practices like yoga and Tai Chi for improving awareness of the body and releasing tension. They concluded the conversation by discussing Daniel's work in psychedelic integration and their shared experiences with psychedelic substances.More Info At https://www.TruthSeekah.com/Daniel-Shankin
TruthSeekah and Daniel had a wide-ranging conversation about spirituality, psychedelics, and personal experiences. They discussed the definition of a shaman and the importance of new terminology to describe modern spiritual experiences. They also shared their experiences with plant medicines and the importance of treating these substances as sacred tools for healing and growth. The conversation covered a variety of spiritual practices, including cow cakow ceremonies and ayahuasca ceremonies. They also discussed the interplay between nature and human experience, the concept of the afterlife, and the importance of gratitude and appreciation. Daniel emphasized the importance of physical practices like yoga and Tai Chi for improving awareness of the body and releasing tension. They concluded the conversation by discussing Daniel's work in psychedelic integration and their shared experiences with psychedelic substances.More Info At https://www.TruthSeekah.com/Daniel-Shankin
TruthSeekah and Daniel had a wide-ranging conversation about spirituality, psychedelics, and personal experiences. They discussed the definition of a shaman and the importance of new terminology to describe modern spiritual experiences. They also shared their experiences with plant medicines and the importance of treating these substances as sacred tools for healing and growth. The conversation covered a variety of spiritual practices, including cow cakow ceremonies and ayahuasca ceremonies. They also discussed the interplay between nature and human experience, the concept of the afterlife, and the importance of gratitude and appreciation. Daniel emphasized the importance of physical practices like yoga and Tai Chi for improving awareness of the body and releasing tension. They concluded the conversation by discussing Daniel's work in psychedelic integration and their shared experiences with psychedelic substances.More Info At https://www.TruthSeekah.com/Daniel-Shankin
Sailors and Marines from the 31st Marine Expeditionary Unit come together as a Blue-Greem team to exercise amphibious operations.
Untapped Power: Insights and Wisdom for Collective Transformation in the Yoga Community
In this episode I sit down with Swami Nityananda. Swami Nityananda has dedicated her life and career to helping humans live in joy and freedom. She has spoken to audiences in ten countries and been a featured speaker on NPR and Radio Ireland among other platforms and podcasts. Her work has been supported by Fulbright grants, the National Endowment for the Humanities, and the Andrew W. Mellon Foundation and has received other national and international awards. She was consecrated as a Swami by Swami Shankarananda in 2014.We Break Down: Background Story and Awake Yoga Meditation CenterEvery human has that connectedness Finding your own entry points into meditation Offering all thoughts into the light Breakdown of Kriya Yoga Discussion to help navigate the daily challenges of life What it means to be a Swami Breaking down mastery of SelfRelationship to Teacher and StudentEnergy gets amplified when we mediation in communityPersonal challenges Intergration of University Teaching and Awake Yoga Meditation Center Overview of Awake Meditation Center Vision for self going forwardUntapped Power of Light and KindnessConnect with Swami Nityananda on instagram @awakeyogaandmeditation or on facebook @awakemeditationcenterJoin our free community today!Leave a review and let us know your thoughts on this episode or follow along on Instagram.
Der Podcast „Total Sozial“ ist in der Sommerpause. Doch Sie müssen nicht auf spannende soziale Themen verzichten. Dies ist eine Folge des Podcasts „Date statt hate“, in dem Menschen von ihrem Leben erzählen, die wegen ihres Geschlechts, ihrer Abstammung, ihrer Sprache oder ihres Glaubens oft Opfer von Hass werden. Ali Cukur ist Boxtrainer beim TSV 1860 München und Sozialarbeiter. Burak Bozkurt ist einer aus seinem Team, mit dem er nach Ghana in ein Trainingscamp gefahren ist. Diese Erfahrung hat ihm geholfen, seinen Weg zu finden. Außerdem berichtet Jürgen Pfau vom Bayrischen Fußballverband, wie sein Sport bei der Intergration hilft und Jörg Ammon vom Bayerischen Landes-Sportverband erklärt, was die Verbände tun, damit möglichst jedes Kind seine Sportart findet. Der Kinofilm ["Lionhearted - aus der Deckung "](https://lionhearted-der-film.de/) von Antje Drinnenberg ist auf vielen Streamingplattformen erhältlich.
Returning guest Ruby Fremon (episode 29) has gone through 2 more traditional Shipibo dietas in the Amazon with master plant teachers Ayahuasca and Ayahuma.As Ruby has ventured deeper on her path with Grandmother Ayahuasca, her understanding of devotion, the integration process, and intention setting has deepened as well. We drop in together to explore the nuances that give listeners pro-level tips for navigating the medicine journey.Big thank you to this sponsor of this episode, Schedule35. They are my go-to trusted source for mushroom microdosing products. Check them our their website and use FLOW at checkout to save 15%.Topics covered:Ruby's experience dieting master plant Ayahuma and AyahuascaWhat its like to diet Amazonian master plants in the jungle of PeruNavigating the nature of duality (good and evil) in humanityThe importance moving slowly during psychedelic integrationHow to create space for the integration processDevotion versus discipline on the medicine pathLeaning into the healing journey even when its difficult or “taking too long”How expectations and attachment to outcomes limits the medicine journeyIntentions versus expectations with psychedelicsShow Links:Schedule35 mushrooms for microdosing - FLOW for 15% offBringers of the Dawn [book}Potent Leadership [Ruby's book]Ruby's websiteShow NotesThis episode was produced in collaboration with FWI Media. Check out their beautiful work!If this episode sparked something within, please let me know and leave a review! 1:1 Coaching with LanaInstagram | Facebook | WebsiteModern Psychedelics Integration JournalDISCLAIMER: Modern Psychedelics does not endorse or support the illegal consumption of any substances. This show is meant for entertainment purposes only. The thoughts, views and opinions on this show should not be taken as life advice, medicinal advice, or therapeutic guidance. This episode was produced in collaboration with FWI Media. Check out their beautiful work! If this episode sparked something within, please let me know and leave a review! FREEBIES to support your journey 1:1 Coaching with LanaInstagram | YouTube | Web | Facebook DISCLAIMER: Modern Psychedelics does not endorse or support the illegal consumption of any substances. This show is meant for entertainment purposes only. The thoughts, views and opinions on this show should not be taken as life advice, medicinal advice, or therapeutic guidance.
Today we offer you some first impressions of OVHcloud and how we're seriously considering moving our Light Pentest LITE training class to it! TLDR: It runs on vCenter, my first and only virtualization love! Unlimited VM "powered on" time and unlimited bandwidth Intergration with PowerShell so you can run a single script to "heal" your environment to a gold image Easy integration with pfSense to be able to manage the firewall and internal/external IPs Price comparable to what we're paying now in Azure land
Joe Biden doomed United States
As we honor MLK Day, Maiysha chats with civil rights legend and renowned journalist Charlayne Hunter-Gault. She shares with Maiysha gut-wrenching stories of being the first Black student to attend the University of Georgia; from dorm life to life-threatening attacks, her stories cement her place in the history book and anthology of extraordinary, brave Black women In her new book "My People," Hunter-Gault tells her own heroic story as well as the iconic and history-making people she has reported on worldwide throughout her decades-long journalism career. See omnystudio.com/listener for privacy information.
Nuno Pinto and Jordan Mora of Assmbly Inc join us to talk all about high-end lighting and home automation. They share their experiences getting into the business and tell us about their products. We hear how they would light different rooms, and compare voice automation from the Big Three. Learn about the importance of light and how it can effect energy levels on this episode of The Construction Life. Interested in Assmbly Inc? Check them out on Instagram @Assmblyinc or send them an email at info@assmblyinc.com. And don't forget, its Assmbly, not Assembly. No “e”. Thank you to everyone who recently purchased a copy of Manny's first book, The Amabel Letters. The support has been amazing. Check out the book and find out how to purchase a paperback or ebook copy at www.theamabelletters.com, on TikTok and Pinterest @the_amabel_letters, and Instagram @_the_amabel_letters. Direct questions and inquiries to info@theamabelletters, and remember, reviews are always appreciated. Connecting with The Construction Life Podcast is easy. To send a question Manny's way, text his mobile at 416 433-5737 or email him at manny@theconstructionlife.com. Be sure to include your name and your question may be read on the show. Do you have something to add to the conversation? Email info@theconstructionlife.com to schedule a date and time to join us in studio! TCL has and always will be about giving back to the construction industry.
Have you wanted to meet your inner child and find a way to soothe them and integrate them back into your adult self. By doing this mediation you will find how to become 'whole' again, inviting playfulness and joy back into your life - and also bringing love and protection back to your inner child who has been stuck in a trauma state in the past somewhere. This meditation will relax you, release stuck emotions and create a safe space for you to meet your inner child and reintegrate them back into your adult self, for a fuller healing process.
The Rev'd Cn. Dr. Donald Shepson, Campus Missioner
After a morning spent exploring Cleethorpes, Lexman interviews Clara Sousa-Silva about her work as an intergration chemist in a research lab. They discuss the different ways in which quaternary structurs can be formed and cleethorpes' place in the history of intergration chemistry.
Franziska Ratsch ist Architektin und Spezialistin für nutzerzentrierte Innenarchitektur. Sie konzipiert und entwickelt Arbeitsumgebungen, die wirksame Zusammenarbeit ermöglichen. Sie ist verantwortlich für Designkonzepte und Work Hacks, die ein allgemeines Verständnis für die Nutzung des Raumes durchsetzen. Durch ihre langjährige Arbeit bei der BREAD&butter und Zalando ist sie darin geschult, inspirierende und intuitiv funktionierende Arbeitswelten für ihre Kunden zu schaffen. Sie verfügt über ein großes Netzwerk zu Architekten, Interieur Designern und Möbelherstellern, womit auf wirklich jeden Kundenwunsch eingegangen werden kann. Sie ist immer neugierig auf ihre nächste Aufgabe und stark darin, Lösungen auf hohem Designniveau und Funktionalität zu finden und diese mit einem hohen Grad an Verantwortlichkeit umzusetzen. Franziska denkt groß und handelt schnell, wenn es darum geht, Veränderungen zu schaffen. Studium mit Diplomabschluss Dipl.Ing. der Architektur, Beuth Hochschule, Berlin / Ausbildung zur Bauzeichnerin, Bremen / Verschiedene Projekte (Joint Venture, Auftragsarbeit) mit dem Hasso-Plattner-Institut / Verschiedene Produktentwicklungsprojekte mit Möbelherstellern (firmeneigene Möbellinien, temporäre Fassadenstrukturen) / Fortbildung Prince 2, AXELOS / Weiterbildungen zum Thema 360 -Grad Feedback, Zeitmanagement, Projektmanagement. Ihr Hintergrund ist: Drei Jahre Erfahrung im Bereich Architektur, Wohnungsbau / BREAD&butter: Acht Jahre Erfahrung im Bereich temporäre Architektur und Messebau bei Zalando: Fünf Jahre Erfahrung im Bereich Projektleitung Konstruktion und Entwicklung von Bürogebäuden, Interieur Design und Entwicklung einer nutzerzentrierte Arbeitsumgebung für Mitarbeiter aus verschiedenen Berufsfeldern, sowohl „white“ als auch „blue collar“ / Sankt Oberholz Consulting, Arbeitsweltraum: Mehrere Jahre Berufserfahrung auf strategisch/ konzeptioneller und operativer Ebene / Gründerin von Bimba Pepa – Furnitecture for Kids and Kidults. Ihre Arbeitssschwerpunkte liegen in Veränderungs- und Transformationsprozesse (Lean Change Approach, Prototyping, Intergration der Mitarbeiter in einen Veränderungsprozess) / Neue Organisationsdesigns, Arbeitswelten und -formen (Aktivitätsbezogenes Arbeiten, Remote Work, andere Orte wie Home Office und Co-Working) / Nutzerzentrierte Arbeitsumgebung en (Unternehmenswerte, Mitarbeiterbefragungen) / Optimierungspotential im Raum durch minimale Veränderungen des Nutzerverhaltens / Einführung neuer tätigkeitsbezogener Möbelkonfigurationen / Arbeitgebermarkenführung und -kommunikation (Spatial Branding, Location Branding, Employer Branding, Aktivierungskommunikation) / Nutzerbefähigung durch Workshops & Trainings. Oliver Grande: Nach mehr als 15 Jahren in der Möbel- und Interior Design-Branche in verschiedensten Organisationstypen, vom straff hierarchischen Handwerksbetrieb seiner Lehrjahre über den Mittelstand und einem selbstorganisiert arbeitenden Beratungsunternehmen, ist er zu der Erkenntnis gekommen, dass Menschen sich nicht an Büros anpassen sollten, sondern Büros an Menschen. Schon immer ging es ihm um den Mehrwert, den Räume ihren Nutzer:innen geben können. Heute arbeite er noch stärker als Übersetzer, Kommunikator und Impulsgeber, der Raum und Kultur von Unternehmen verbindet und Orientierung bietet, um Büros zu Orten zu machen, die neuen Ansprüchen gerecht werden. Für Arbeitsorte, die dem “Permanent Beta“ der Arbeitswelt nachhaltig gewachsen sind, beständig wandelnde Strukturen und Prozesse tragen, auf das Geschäftsmodell abgestimmt sind, Zusammenarbeit fördern, das Potenzial haben, Individuen und Unternehmen zu einen, Identität stiften und viel Zukunft versprechen. Es geht ihm darum Orte zu schaffen, die neuer Arbeit würdig sind! Genießt die Energie, die aus kreativer Zusammenarbeit, zwischenmenschlichem Austausch und gemeinsamen Zielen entsteht. Er ist immer auf einer Mission und glaubt daran, dass der Status quo vor allem dazu da ist, hinterfragt zu werden.
In this episode, we talk with Olivia Englehart from SugarU about the Sugar Market and Sugar Sell integration. She explains who, what, when, where, and how to implement these two products together. SugarCRM Services Sugar Market Sugar Sell SugarU Course – How Sugar Market and Sugar Sell Work Together
Darren Kelly and Killian Whelan present the magazine show featuring the Littlewoods National Camogie Leagues and the AIB All-Ireland Club Camogie Finals. Schedule: Latest News (inc. Intergration and Katie Mullen - 0:00) National League Division 1 Review inc. Cork vs Limerick (21:30) AIB Club Finals Preview inc. Oulart The Ballagh vs Sarsfields (47:35) We look at the latest news as integration takes a step forward following the GAA Congress; it's a club finals weekend with two Junior deciders in Drogheda on Saturday; Katie Mullen chasing a camogie All-Ireland before hockey World Cup, and some big results for Cavan and Wicklow in the National Leagues. The middle part of the show focuses on Division 1 and after Cork's massive win over Limerick, we worry for the Shannonsiders, and also Offaly who took a heavy beating from Dublin. Can anyone challenge the big four this year we ask, though Clare have put up their hand. And finally, it's Croke Park Sunday with two games live on television. Can Salthill/Knocknacarra complete a fairytale or will St. Rynagh's make history at intermediate? And who can come out on top in one of the most anticipated rematches in history when Oulart The Ballagh look for a repeat win over Sarsfields == ‘SportsDaz Camogie' broadcasts every week on sportsdaz.ie · To watch back the meeting of Cork vs Limerick last Saturday and to hear the match report, click HERE · Cover Photo (c) Hannah Shields – Clonduff Camogie Club · To read report and get reaction from Kilkenny vs Clare, click HERE · Theme Song (c) josephmcdade.com/music · #SportsDaz
In June 2021, as part of back-to-back commencement exercises, Gonzaga’s 36th President, Fr. Stephen Planning took the opportunity at his final graduation ceremony to award a special Doctorate for the first time during his tenure. As many of you know, Gonzaga College High School’s historic charter allows for the awarding of honorary degrees. The recipient: Gabe Smith '54, the first African-American to graduate from Gonzaga. As Black History Month is being celebrated this February, this week in S1E20, we celebrate Dr. Gabe Smith. Just how did the young student from St. Peter Claver’s Parish, in Southern Maryland end up, 80 miles away on Eye Street? And how was Gabe treated once he arrived? And after the rigors of four years at Gonzaga, did the future pharmacist feel prepared for Howard University? Gabe’s shares the answers, and his appreciation for God and Gonzaga in Episode 20 of the Echo Ever Proudly podcast.See omnystudio.com/listener for privacy information.
Carey Green is Dr. Brad Miller's guest on Episode 181 of "The Beyond Adversity Podcast." In this episode, Carey shared how his experiences working in a ministry, dealing with different people, and moving to foreign places have been somewhat a challenge for him. Carey Green was a pastor in various churches across the US. It was a rewarding and humbling role that he would never trade. In 2011, Carey began to feel the end of his calling in that role. His conviction was (and is) that the church deserves a Pastor whose heart is entirely in part – and he was not. It was the right move. Carey experienced disappointment, pain, and discouragement when building a church in that particular location. He and his wife were called in to help save a church when the former pastor's marriage fell apart. Carey described the event as being like watching the death of a church with his own eyes. He attempted to fix things with the church and its members at first, but as time passed, he realized that some things were simply beyond his abilities to mend. He had the impression that it wasn't just working for him but also for the church. He gradually realized that fewer people were there, and the crowd diminished. He resigned, knowing it wouldn't work, persuading members to choose someone more suited and with whom they could connect. The church subsequently closed down after about a month. He needed to find a new job, and he was fortunate enough to get recruited as a cable man, where he goes into people's backyards and disconnects wires if they have not paid their overdue account. He had to take on additional occupations, even if they weren't what he had planned on doing, to put food on the table and keep in mind that they are only renting a house for his entire family. Carey eventually returned to ministry and was sent to another church, where he was determined to make it work no matter what. It was almost as if they were having an open conversation with the new church members since they were asking for an opinion on how Carey should govern the church. They held family gatherings where individuals individually spoke up about specific issues, and some issues were resolved only due to this act of communication. For those spiritually inclined Christians struggling with some elements of life, Episode 181 of The Beyond Adversity Podcast is a must-listen. We have all been in situations where we feel out of control and hopeless because we are left with few options, but as Carey says in this episode, we must never forget that God will not allow anything wrong to happen to us and that we must trust Him that He will provide for all of our needs as long as we are willing to take action because Faith without Action is useless. “The Beyond Adversity Podcast with Dr. Brad Miller is published weekly with the mission of helping people “Grow Through What They Go Through” as they navigate adversity and discover their promised life of peace, prosperity and purpose. Website: https://podcastfasttrack.com/ (https://podcastfasttrack.com/) | https://pft-service.youcanbook.me/ (https://pft-service.youcanbook.me/) Youtube: https://www.youtube.com/c/Podcastfasttrack1 (https://www.youtube.com/c/Podcastfasttrack1) LinkedIn: https://www.linkedin.com/in/careyngreen/ (https://www.linkedin.com/in/careyngreen/) Instagram: https://www.instagram.com/careyngreen/?hl=en (https://www.instagram.com/careyngreen/?hl=en) Twitter: https://twitter.com/PodcastFast (https://twitter.com/PodcastFast)
The LeoFinance weekly AMA is a participatory podcast brought to you by @khaleelkazi, @nealmcspadden and @scaredycatguide. It provides the latest developments in the world of LeoFinance and CUB Finance. Everybody is welcome to participate using Facebook, Twitter, Discord text chat, Youtube chat and other social media platforms. The AMAs are scheduled at 10AM CST on Tuesday. Episode #32 covered the following topics: * Leo Lightning update(0:00 – 3:30) * Popular-feed / promoted-feed / curators on LeoFinance (3:30 – 5:39) * Update PolyCUB/Mobile App/Text search (5:39 – 8:52) * HiveStats (8:56 – 11:30) * CUB Bonds (11:30 – 15:02) * IDO #2 (16:35 – 19:28) * Biggest mistakes and learnings of 2021 (19:28 – 22:02) * CUB Airdrop (22:02 – 23:35) * LeoFinance DAO (22:35 – 27:55) * Thorchain Integration (27:55 – 36:10) * CUB/LEO on central exchanges (36:10 – 39:42)
Link zum Discord: https://discord.gg/VjzedYFGkuPhotovoltaik Anlagen sind eine der tragenden Säulen zur Bekämpfung des Klimawandels und für die Energiewende im Elektrizitätssektor. Während wir heute Solar PV meist auf Dächern und auf größeren Flächen neben der Autobahn sehen, werden wir Solar PV in Zukuft auch an anderen Orten als “integrierte PV” sehen. Wie Solar PV funktioniert und wie diese Intergration geschehen kann lernt ihr in dieser Folge mit Anna Heimsath vom Fraunhofer ISE. Am Fraunhofer ISE leitet Anna die Abteilung "Analyse, Module und PV Kraftwerke" und ist somit eine der Expert*innen im Feld zu Solar PV. Außerdem werdet ihr in dieser Folge lernen was Agri-PV und Floating PV ist und wie wir unsere Solar PV Ausbauziele erreichen können. Wenn ihr noch mehr zu dem Thema erfahren wollt, haben wir hier noch ein paar Links zusammengestellt: fakten-zur-photovoltaik-in-deutschland https://www.ise.fraunhofer.de/de/veroeffentlichungen/studien/aktuelle-fakten-zur-photovoltaik-in-deutschland.html Wege-zu-einem-klimaneutralen-Energiesystem https://www.ise.fraunhofer.de/content/dam/ise/de/documents/publications/studies/Fraunhofer-ISE-Studie-Wege-zu-einem-klimaneutralen-Energiesystem-Update-Zielverschaerfung.pdf global-market-outlook-2021-2025 https://www.solarpowereurope.org/global-market-outlook-2021-2025/—Neue Folgen des enPower Podcast bekommt ihr an jedem zweiten Sonntag überall dort wo es Podcasts gibt. Wenn ihr uns unterstützen wollt, damit wir in Zukunft noch besseren Content liefern können, schaut gerne in unserem Shop unter https://www.enpower-podcast.de/shop oder auf https://www.patreon.com/enpower?l=de vorbei.
Catherine and Francis are excited to speak with Kim Machado, a psychotherapist and trained trauma expert, about the complex intersection of eating disorders and trauma. Kim shares how the subjective experiences of trauma impact individuals in various ways, and that many use maladaptive behaviors to tell a story of their trauma. Her approach in working with those who experience trauma is empathetic, compassionate, and can be described by one of her favorite quotes from Alice in Wonderland, "when you can't look on the bright side I will sit with you in the dark." We are so appreciative of Kim's time, energy, and expertise. We hope you enjoy!CW: trauma experiences, eating disorder behaviors, mention of abuse, mention of complex trauma, self-harm behaviors
EP 233 of the Leave Your Mark Podcast is a return engagement with my friend from down under, David Joyce. David has been a real leader in the world of human performance for a very long time now. I value a great deal his insights and learnings through a career at every level of the performance spectrum. In this wonderful conversation, we get into his change in life direction, his choice to do an MBA, his growth in fatherhood, and the work he and his co-editor Dan Lewindon have been doing through COVID to bring the second edition of their groundbreaking text "High-Performance Training for Sport" to fruition. Herding this incredible group of practitioners to the creation of a legacy textbook that really summarizes the changes in the landscape of performance, the newest research findings, and the future expectations of things to come. It's a conversation about the passions of this man's life and our common beliefs in the integration of human performance.
Kirk was in Memphis to call a game this past weekend and he shares his thoughts on visiting the Lorraine Motel for the first time. Visiting that historic site was overwhelming. Jason shares his visit to the motel as well. Discussion on the NFL taunting rules and reaction to an article suggesting that the rule is a way to control black athletes. Keyshawn Johnson, Former WR & Bob Glauber, NFL Writer - Authors of 'THE FORGOTTEN FIRST: Kenny Washington, Woody Strode, Marion Motley, Bill Willis, and the Breaking of the NFL Color Barrier'This book tells the story of each of the four pioneers of the NFL and all they had to deal with beginning in 1946. Bob had the idea and approached Keyshawn during the unrests last year to write the story of the men who were the first African-American NFL players. This is a part of history that isn't taught in the schools. Folks sometimes take for granted that the NFL is a highly integrated sport. Bob wanted to explore when the league first became integrated. Many people do not know Kenny Washington is the first black NFL player. The NFL played many games in the south just to keep black players from playing. Keyshawn draws the parallels to what happened then to what's happening now. Wonders how quickly the NFL would have been integrated had the NFL been as popular or more popular than baseball at the time. The NFL had to be forced to integrate, unlike the Dodgers who did it willingly with Jackie Robinson. The guys are pushing, along with help from the NFL, to get the message out there of these four men. Hopefully this book educates and show how far black players have come in the NFL.
We discuss Sonic's new grilled cheese burger, whether Darrell K Royal and Bibb Falk were racists, and Are You Smarter Than Jason Dick. See omnystudio.com/listener for privacy information.
Whether we acknowledge it or not, race, sex, gender, class, and privilege are all part of our daily lives no matter who we are, what we look like, or where we're from. But too often we don't talk about these issues for fear of saying the wrong thing, or that the conversations will be difficult, bitter, and even painful.
In our hopeful conversation, we talk about the beauty in cannabis as a psychedelic, the power of “set & setting”, psychedelic integration, patience as supreme grandmaster teacher, pragmatic tips for living healthy, happy, and strong service-driven lives, the value of engaging shadow work and finding the gift in our darkness. Libby Cooper, the CEO of Space Coyote inspires us to explore the lighter, playful side of life, she graces us with her optimistic perspective and outlook, and plentiful seeds of accelerated transformational growth. Show Notes Libby Cooper/Space Coyote We Discuss; -How have psychedelics impacted your journey? [4:13] -How do you feel about set and setting [5:49] -Intergration practices from day to day [16:01] -Seeking out discomfort [22:24] -What keeps people from leaning into their edge? [29:25] -Where do you see psychedelics in the next 5 years [40:32] -Where to find Libby and Space Coyote [47:22] Helpful Resources: Yvon Chouinard “Let My People Surf” Steven Kotler — Cannabis & Creativity Dr. Dan Engle — Full Spectrum Medicine Akshay Nanavati “Fearvana” Steven Pressfield “War of Art”, podcast with Aubrey Marcus Brene Brown's Paradoxical Power of Vulnerability Connect with Libby Cooper & Space Coyote Space Coyote Website - https://www.spacecoyote.org Space Coyote Tik Tok - https://www.tiktok.com/@thespacecoyote Space Coyote Instagram -IG @spacecoyoteofficial Libby Cooper Instagram - IG @libbycooper Worth The Fight Links: IG: @worththefightbook Worth The Fight book: Purchase your copy on Amazon, Kindle, or Audible Worth The Fight podcast iTunes easy review link Matt's 1-on-1 Worthy Fight Empowerment Coaching To financially support our NFP Worth The Fight service project with monthly donations on Patreon
Today I tackle my most popular questions from the listeners. Diet, Integration of your new Cane Corso to your pack, E-collars and do they work and crate training.
.Grabovoi codes, energy of everything, intergration, 5IR
Ashley McKella is MD Agent Hub: a revolutionary CRM for the Estate & Letting Agency industry. Agent Hub helps Estate & Letting Agents in the property sector organise their work, connect with other professionals, reach a wider audience, and close more deals, Agent Hub is already changing the way the property sector operates. Agent Hub is the only CRM system with a Multiple Listing Service (MLS), working alongside Salesforce dedicated to making waves in the UK property industry. As a fully cloud-based service, you can access the system from any computer, mobile, or tablet. This makes it perfect for in-office and field-working teams who can share information in real time. Tailor this service to your individual needs with over 1,800 apps available when you join the Agent Hub community. Anyone in property connect, collaborate and work together online and on the move. You get all the benefits of a cloud-based CRM, network of property professional contacts alongside the functionality of an MLS that enables you to manage contacts and content all in one place. Whether you’re a field-based rep or an office-based agent, you can all log into the same system and instantly access and share the information you need to generate leads and close deals. Feel free to contact Ashley on Ashley@agenthubltd.com for more information. NO MONIES OR CONSIDERATION WERE GIVEN BY ASHLEY OR AGENTHUB FOR THE INTERVIEW
I've decided to share some of the books I've been reading with you all by reading them aloud to you all. It will help improve my oration skills. Right now we are reading The Blackman's Guide to Understanding the Blackwoman by Shahrazad Ali. This Chapter goes into the blackwoman's idea of what social integration was intended to do and what actually came of it. You can purchase the book on Amazon (Link Below): https://www.amazon.com/Blackmans-Guide-Understanding-Blackwoman/dp/0933405014 --- Support this podcast: https://anchor.fm/colelogicpod/support
Have you ever thought that you could be so much more efficient if only your apps could share information automatically? Well, they can! Learn how to save time and money with API Integrations.
This week, we attempt to clear up all the confusion around WhatsApp's new privacy policy in Watchtower Weekly, and admire the takedown of the now-infamous botnet, Emotet.Stopping by for This Week at 1Password is Rick Fillion. Rick is VP of Engineering for 1Password.com and shares some exciting new integrations for the admin console, and what that might mean for the future.If that's not enough to get you reaching for your AirPods, 1Password's very own Cat Friend joins the line-up, to answer all your questions in #Ask1Password and run Play Your Passwords Right: Gaming Edition (Round 2)
Was hat ein Weckmann mit Sankt Martin zu tun? Oder war das der Stutenkerl am Nikolaus? Wie sollen wir uns in Deutschland integrieren, wenn die Deutschen selbst durcheinanderkommen, wenn sie von einem ins nächste Dorf ziehen. Da ist Katja froh, dass sie dieses Jahr keine Lieder beim Kita-Umzug mitsingen muss. Währenddessen vermisst Olgas Familie das Martinsfest und überlegt sich eine coronakonforme Route: von der Küche zum Bad, weiter ins Wohnzimmer und in den Flur, raus in den Garten bis hin zum Schuppen. Laterne hoch!
Episode 50 | Vertical Intergration A.k.a "V.I" by Conch Boyz
Subscribe on iTunes, Spotify, Google, Deezer, or via RSS.Code Review Workshops:Michaela’s Code Review WorkshopsWebinar: Michaela's secret sauce for fast and effective code reviewsLinks:Kent C. Dodd’s testingjavascript.comAll Testing Blog Posts of KentTesting PyramidWrite Tests. Not too many. Mostly integration.Test Confessions: How Plug-in Systems are tested – StudyTesting Implementation DetailsWrite fewer, longer tests
It's funny, we struggled a bit to wrap words around one of our favorite spiritual practices!! We think it's because it must be *experienced*. So, this episode is just the first of more conversations to come about yoga. One highlight from this episode: what happens on your mat translates off your mat...Intergration!
Here I go with Part 3 to The Intellectual Series for Black History Month. This time I hit my guys from Thee Breakroom Podcast to come thru and join the convo. "Good PAC/Bad PAC" was dope, so we back at it with "Segregation vs Integration 2020"...an in-depth look at whether integration has worked and whether or not segregation could ever work. Of course the detours added some super interesting angles to the convo. Tune in and enjoy!
Chris and Tyler start off the episode like every other, with a nice cold one....Beer: Flying Dog - Operation Breakfast: All-You-Can-Eat Coffee Vanilla Stout (Coffee Stout, 9.1% ABV, ): Inspired by all-you-can-eat breakfast, this beer has flavors of oatmeal, waffles, buttered toast, and café lattes with cocoa-vanilla whipped cream. As always we would like to thank Oakbrook Brewing Company for being our sponsor.Gritty takes a shot at the Houston Astros:https://www.tmz.com/2020/01/22/philadelphia-flyers-mascot-gritty-roasts-houston-astros-cheating-scandal/A list of the Top 50 Cheaters in baseball history:-https://bleacherreport.com/articles/622103-mlb-power-rankings-alex-rodriguez-and-the-top-50-cheaters-in-baseball-history#slide50A Black history of Baseball:https://ourgame.mlblogs.com/baseball-integration-timeline-b289bc04ca12Make sure to check out or kickstarter to help send us to Fresh Fest Beer Fest: https://www.kickstarter.com/projects/alternativeblacks/alternative-blacks-go-to-fresh-fest-beer-fest
Join Us in The Blue Room !!! * Ms.Blue's take on The Heart Of The Soul * Contact MsBlue: question4blue@gmail.com Readings | Know Your Magic Audio WorkBook | Know Your Magic Oracle Deck | Self Invested Workshop | Akashic Records Readings | Radio Host | Medium | Intuitive Healer | Psychic - Medium | Intuitive Healer | Psychic |Certified Holistic Practitioners | Metaphysician | Cosmic Creator | Soul Contracts | Pass Lives https://www.facebook.com/pages/The-Remix-Radio/114209487075 | http://tinyurl.com/MsBlueRemix | @theremix_radio Do The Work ! http://msblueblast.com/digital-rituals-courses/ Contact: Bro Balah: balahtheexperience@gmail.com Soul Alignment Reading | Soul Cycles | Numerology | Astrology | Astrologer | Radio | Music | 13 | Soul Body | Soul Alignment Reading | Soul Cycle CalendarsThe Mystical | @balahtheexperience_
REAL SPORTS TALK, M-F 6:00PM
Moe Odele is a cofounding parnter at Vazi Legal and the founder of the widely popular community, Scale My Hustle. Moe is an international attorney experienced in Business and human rights, Innovative financing, Impact Investing, Gender & inclusive finance, ESG Advisory (Due diligence & Intergration), Private Equity and Venture Capital, FinTech, Startup advisory, and Social entrepreneurship. She has worked with entrepreneurs, funds, family offices and foundations who are dedicated to solving some of the most pressing challenges in emerging markets.
In this week’s episode of MBDR, I spoke with Anne Martin Vetrano, an award-winning writer and journalist, who spent 30 years in broadcast news with WXVT and WABG television stations in Greenville, Mississippi. Anne is the author of Delta Hot Tamales: History, Stories, and Recipes. She has written for such magazines as Life in the Delta, Eat. Drink. Mississippi, The Sip, and Delta Magazine. In our conversation, we compare our experiences as students in the public and private school systems in the Delta. I think you’ll enjoy the discussion. For more about Anne and a link to purchase her book, please check out our website at mississippiborndeltaraised.com or email me at daphne.robinson.jd@gmail.com and I will add you to our email list. Please join me in welcoming Anne to this week’s episode of MBDR.
The video version of the show: https://youtu.be/h4Cd2tiCIxg Rob Waller is today's guest on the How To Code Well podcast. Rob talks about testing code and testing an application for failures and expected outcomes. Rob Waller is a software developer with a background in managing development teams. He is also a speaker and has given many talks on software testing. We discuss the best way to test legacy code, how to explain the importance of testing to stakeholders and the difference between testing for failure and expected outcomes. We also talk about Rob's interview on CNN. Rob created an application that found fake followers on Twitter. This app was used during a previous US election by many journalists. Follow Rob Waller Twitter: https://twitter.com/RobDWaller Robs talks: Test to Break Principle: PHP South West https://youtu.be/vRtioaertzU PHP UK Conference https://youtu.be/JDwFyRXbVHQ *** My Programming Courses *** Docker In Motion http://bit.ly/2vvz2sA Hands-on Microservices In Python - Packt Publishing: http://bit.ly/2S6aMYB - Udemy: http://bit.ly/2tD8S3Q *** Programming resources *** Programming books from Manning Publications: http://bit.ly/2BIrEx Udemy courses https://www.udemy.com/user/peter-fisher-8/ Skillshare courses https://www.skillshare.com/r/user/howtocodewell *** Follow How To Code Well *** Facebook: https://www.facebook.com/howtocodewell Twitter: http://twitter.com/howToCodeWell Youtube: http://bit.ly/2wf9ufB Instagram: http://instagram.com/howtocodewell/ Website: https://howtocodewell.net *** Subscribe to the news letter *** http://tinyletter.com/howtocodewell *** My Talks *** Using a Framework or Not - PHP South West https://youtu.be/T8R3YTrqt6U How To Put Your Docker Images On A Diet - PHP South West https://youtu.be/uiABt9axPNo Software Complexities - Peter Fisher - PHPSW: Learning About Complexities, August 2018 https://youtu.be/ZQ6AkyvEaHE DISCLAIMER: This video and description contains affiliate links, which means that if you click on one of the product links, I’ll receive a small commission. This helps support the channel and allows me to continue to make web development videos like this. Thank you for the support! #testingToBreak #podcast #webDevelopment
Two Chicks One Mic discuss the Royal baby, lack of unification in the Democratic party, Intergration, Grey sweat pants season, and trying to lock down young rich and famous people
Two Chicks One Mic discuss the Royal baby, lack of unification in the Democratic party, Intergration, Grey sweat pants season, and trying to lock down young rich and famous people
Two Chicks One Mic discuss Brett and Cody's sexual assault allegations, Drake grooming young girls, and Kanye's crazy ass!
Two Chicks One Mic discuss Brett and Cody's sexual assault allegations, Drake grooming young girls, and Kanye's crazy ass!
Philip Haddad joins us for a discussion about the re-intergration of ex-convicts into society and some of the struggles they face. But there is help. Strength in Peers, a non-profit organization here in Harrisonburg is leading the way to help ex-cons get back on their feet, remain drug free, and reducing the risks of violating the terms of their release. Bobby Butcher will also be on hand to give us a sneak peak into his latest track recorded earlier this week at Fat Cat Studios. We want to hear from you. Studio lines will be open. 516.387.1941
The Elephant in conversation with Dr Kizza Besigye, Uganda's opposition leader.
Recorded live at Cisco Live U.S. Learn the comprehensive new capabilities offered by DNA Center Platform that allow integrations with external applications that make networks more agile and align closely with business priorities. Justin Cohen, Cisco Toronto Innovation Center, leads a conversation with Cisco DNA Center's Prakash Rajama and Sharmila Phadnisni as well as Raakhee Mistry, Sr. Director of Marketing for DNA. For more, check out this link: http://cs.co/9006Dm6lp
http://www.b2bRevenue.com http://www.dialsource.com Video Emails by Covideo = http://www.Covideo.com A.I. Call Analysis by Gong.io = https://www.gong.io/bt Audible 30 day Free Trial: http://www.audibletrial.com/BrutalTruth Check out my YouTube channel and watch my Free Sales/Social Selling Course. https://www.youtube.com/subscription_center?add_user=MaverickMethod Acuity Scheduling Free Trial: https://acuityscheduling.com/?kw=YToxMzE1MDM2MA%3D%3D Listen to The Sales Questions PodCast: https://itun.es/i67d3Ry Listen to The B2B Revenue Leadership Show: https://itunes.apple.com/us/podcast/b2b-revenue-leadership-show/id1174976428?mt=2 Join The Gaggle and Spread The Brutal Truth: http://gaggleamp.com/i/xfpnkxv Get a 10% discount code = Bburns10 http://www.findthatlead.com
Check out the video segmentsPart 1 - https://youtu.be/Htj3ZadrKYUPart 2 - https://youtu.be/B2WreLV5SZ8Part 3 - https://youtu.be/7uYBZdNTDsU
Check out the video segmentsPart 1 - https://youtu.be/Htj3ZadrKYUPart 2 - https://youtu.be/B2WreLV5SZ8Part 3 - https://youtu.be/7uYBZdNTDsU
Konflike können viele Ursachen haben und die verdeckten muss man zuerst erkennen. Offenheit und Transparenz können viele Konflikte lösen und langfristig verhindern. Wie man durch Intergration vermeintlich unlösbare Konflikte doch für immer lösen kann. Die meisten Konflikte lassen sich vermeiden. Mehr Informationen gibt es unter http://visionselling.smile2.de/konflikte-professionell-l%C3%B6sen-sales-up-call-weckruf-fuer-ihren-vertrieb.
Miss Dyanne Mason Jones elaborates on her book about "Hello World What The Hell" A Baby Boomer's Life Journal of Segregation,Intergration, and Salvation.
Sirpa Alho, entisiltä nimiltään Sirpa Ryd ja Sirpa Suvimetsä, on työskennellyt Ruotsisssa opettajana sekä kulttuuritöissä. Kesäpaikka on Samoksella, saarella, jonne pakolaiset tulevat meren yli. En vain minä auttanut pakolaisia. He auttoivat minua. Me autoimme toisiamme.Sirpa AlhoSoilin seurassa tavataan joka viikko uusi vieras, josta tuleekin sinulle tuttu.Sirpa kirjoitti kirjeen:Olen tytär äidille Kemistä ja isälle Lumivaarasta, Karjalasta. Karja-Pohjolasta (sen ajan Scan) sanoi äitini. Karjalan poika ei ollut unelmavävy Pohjolan suvulle. Karjalasta tulleet evakot nauroivat liikaa, heillä oli kummallinen ruoka ja murre oli aivan outo.Isä rakensi voimalaitoksia. Omakoti Lautiosaaressa jätettiin kun olin 8 vuotta. Kemijoki-Iijoki, uusia kouluja, mutta samat kaverit, koska kaikki muuttivat samoihin uusiin kohteisiin. Oltiin kylläkin outoja paikkakuntalaisille.Kun olin 13 vuotta, oltiin kesälomalla Nisbackassa Helsingin ulkopuolella isän siskojen luona. Silloin vanhempani jättivät voimalaitokset ja päätivät muuttaa Nisbackaan. Siskoni ja minä jätettiin lapsettomien tätini huomaan ja vanhemmat lähtivät hakemaan muuttokuormaa. Ei saatu sanoa hyvästejä ystäville.Murrosikä Korsossa 60-luvulla. Kova paikka sopeutua teryleenimekossa Jamesporukkaan. Kävihän se. Yhteiskoulu oli puoliksi yksityinen ensimmäistä vuotta kun tulin. Duunarin kakara otettiin pakolla vastaan. Pehmosta kovikseksi.Lapsenlikkana Helsingborgissa 17-vuotiaana, huono ruotsinkieli lukiossa. Kunnianhimoinen äiti. Seuraavat kesät Sankta Marian mielisairaalassa töissä.Ylioppilaaksi 60-luvun lopulla. Vallankumous houkutteli. Sovittiin kavereiden kanssa, että lakkia ei panna päähän, kun julistetaan, että nyt olette ylioppilaita. Kaikki valkoisissa, minulla punainen tätini (helluntalainen) virkkaama mekko, punainen virkattu baskeri, punaiset sukat ja kengät. Olin ainoa, joka ei pannut valkolakkia päähänsä. Rehtori raivosi, rehtorin vaimo itki. Ruokalan porukka kätteli.Äiti halusi minusta asianajajaa. Lähdin Ruotsiin, meri välissä. Vahva äiti.Lundin yliopsto. Ajattelin olla siellä yhden lukukauden ja tulla takaisin Valtiotieteellisseen. Vallankumous houkutteli. Kommunistisk Ungdom, Vänsterpartiet, toimitsijana. Lahdenperä houkutteli opettajaksi Olofströmiin. 4 vuotta yläasteella. Naimisiin tukholmalaisen Kennethin kanssa. Hän kesti Olofströmiä vajaat kaksi vuotta. Muutto Luulajaan. Koska EN IKINÄ muuttaisi Tukholmaan. 3 vuotta Luulajassa ja sitten Rinkeby, 1984. 3 lasta. Ruohonjuuri harrastusta asuinalueella.Töissä Svenska Fredskommittessa. Muutto Östermalmille, 1991. 10 vuotta Östermalmilla.Töissä Spånga-Tenstassa. Kultur och Intergration. Kvinnocenter i Tensta, Sahra, Mona ja minä Dubaissa.PfMI, Linkworkers- yhteistyötä Irene Molina, Nihad Buran, Roger Andersson, Aleksandra Ålund ja monta muuta.Urban Futures - töissä - Kulturdepartementet.Avioero, ja sitten monen vuoden jälkeen naimisiin suomalaisen miehen kanssa. Todella haasteellista.Yhteinen harrastus second hand - kirppiskierroksia riittää!Asuttu Tukholmassa, vietetty pitkiä aikoja Samoksella ja kesät Heinolassa.Pitkäaikainen ystäväni Jasim Mohamed tarvitsi apua Samoksella, kun pakolaisvirrat aukesivat.Sirpa AlhoHaastattelijana Soili Huokuna soili.huokuna@sverigesradio.se
TonyAbad is a Lecturer on International Economic Law at the Ateneo de Manila Law School. He is a member of the Philippine Bar and has been in legal practice for over sixteen years. Mr. Abad is also the Managing Director of T A Trade Advisory Services, a firm specializing in international trade law and providing technical advice and assistance to multinational corporations and business enterprises, as well as international organizations, government and academic institutions, on matters of international trade policy and regulation, competition law and policy, and good governance.
did intergration work for us and is it a real solution
In this installment of the EPC's Race and Education series, we will discuss the aftermath of the Brown vs. Board of Education ruling. With a mandate to expediate intergration given by the Supreme Court, many states, universities, and school districts in the South use a variety of tactics, including violence, to ensure "segregaton now, segregation tomorrow and segregation forever." Join us at EPC as we discuss policy, race, and law in a post Brown era.
In this installment of the EPC's Race and Education series, we will discuss the aftermath of the Brown vs. Board of Education ruling. With a mandate to expediate intergration given by the Supreme Court, many states, universities, and school districts in the South use a variety of tactics, including violence, to ensure "segregaton now, segregation tomorrow and segregation forever." Join us at EPC as we discuss policy, race, and law in a post Brown era.
On December 1st 1955 the civil rights movement was born when Rosa Parks refused to give up her seat on a city bus in Alabama. Now 55 years later we ask the question Did Civil Rights or Integration kill Black America? Did integration do more to help or hurt Black People? Call in and let us know what you think. Also we will talk World News, Politics and Celebrity Gossip on the CCShow. Saturdays @ 12:30pm CST
August 19, 2010 — Einstein researcher Sophie Molholm, Ph.D., discusses an autism study that showed an empirical link between multisensory integration and autism. The study, published in the August 19 online edition of Autism Research, could lead to the development of an effective means to evaluate various autism spectrum disorder therapies.
Manufacturing Thursdays Seminars, Institute for Manufacturing
The design of the supply network, linking suppliers, producers, distributors with their customers is becoming ever more important. Globalization, new routes to market and rapid technology change have resulted in changing industry structures, often involving a fragmentation of the traditional supply chain. Dr Jag Srai details some of the exemplar models identified in recent research projects within the Centre for International Manufacturing.
Bei dieser WM tauchten plötzlich Themen auf, die wir alle kennen, aber beim Fußball nicht unbedingt erwartet haben: Patriotismus und Integration. Und alles liegt an den Flaggen, ohne die eine WM nunmal nicht sein kann. Wie sollten wir uns sonst auseinanderhalten??? Ob wir nun Patrioten sind, fragt man sich gerade in den Medien. Dieser Frage geht die KKPodShow heute nach. Und überlegt außerdem, wie die Sache mit der Intergration nach der WM weitergehen könnte. Zum Thema "Deutsch für Kinder anderer Muttersprache" hier ein TIPP: "Hennings Haus" (Verlag: FWU Institut für Film und Medien) Die DVD richtet sich an Kinder im Grundschulalter. Henning ist ein komischer Typ mit magischen Fähigkeiten. Gemeinsam mit Kindern verschiedener Herkunft vermittelt er den jungen Zuschauern einfach und verständlich das Grundwissen der deutschen Sprache. Es gibt viele Filme und Kapitel, die es auf leichte Weise zu betrachten und zu erarbeiten gilt. Die DVD kann gekauft werden oder von Schulen über die bekannten Medienzentren ausgeliehen werden. Die KKPodShow heute mit Musik von: - Don Betto "Il Salsa" - Azat "Ankara 06" - T. Nile "Get Together" *"Some of the music provided tonight from the PodShow Podsafe Music Network. Check it out at 'music.podshow.com'"* ____________________________________________________________
Fakultät für Biologie - Digitale Hochschulschriften der LMU - Teil 01/06
Die vorliegende Arbeit wurde in zwei Bereiche gegliedert. Der erste Themenbereich beschäftigte sich mit der Transformation von pflanzlichen Mitochondrien bzw. mit den Voraussetzungen, die erfüllt sein müssen, um Mitochondrien höherer Pflanzen zu transformieren. Im zweiten Themenbereich wurde die Regulation der Lysinbiosynthese erstmals mit Hilfe der Plastidentransformation modifiziert sowie ein universeller plastidärer Selektionsmarker entwickelt und etabliert. Mitochondrientransformation- In dieser Arbeit wurden zwei Strategien zur Transformation von Mitochondrien höherer Pflanzen entwickelt. Zum einen wurde ein mitochondrienspezifischer Ansatz gewählt, d.h. es wurden Selektionsmarker verwendet, die eine hemmende Wirkung auf die Atmungskette der Mitochondrien besitzen. Zum anderen wurden in Anlehnung an seit die seit 1990 erfolgreich durchführbare Plastidentransformation generelle Inhibitoren der Proteinbiosynthese verwendet, welche durch die Produkte der entsprechenden in die Mitochondrien eingebrachten Resistenzgene detoxifiziert werden sollten. Markergenstrategie- Geeignete Selektionsbedingungen zur Identifizierung von mitochondrialen Transformanten wurden bei Tabakblättern und Tabakprotoplasten mit den Antibiotika Blasticidin, Chloramphenicol und Hygromycin als Hemmstoffe ermittelt. Aus Transformationen mit den mitochondrialen Transformationskassetten pBMhph II, pBMhph III und pBMhph IV konnten über 200 Hygromycin-resistente Linien selektiert und charakterisiert werden. PCR- und Southern-Analysen lassen bei mindestens 7 Linien eine zielgerichtete mitochondriale Integration des Transgens vermuten. Möglicherweise sind aber nur wenige Kopien des Transgens in das Mitochondriengenom inseriert bzw. repliziert worden, was eine eindeutige Charakterisierung erschwert. Bei einer großen Anzahl resistenter Linien handelt es sich wahrscheinlich um funktionelle zielortfremde Integrationen der Transformationskassetten in die Plastiden-, Kern- oder Mitochondriengenome. Mitochondrienspezifischer Transformationsansatz- Die in dieser Arbeit entwickelten mitochondrienspezifischen Transformationsvektoren pUMmyx II, pUMglu II, pUMarg II und pUManti II enthalten ein Fragment des mitochondrialen cob-Gens. In dieses cob-Fragment wurden Punktmutationen eingeführt, die zu einer Veränderung der Sekundär- bzw. Tertiärstruktur des Apocytochroms b führen. Bei einer korrekten homologen Integration dieses modifizierten cob-Fragmentes in das Mitochondriengenom sind die Inhibitoren Antimycin A, Myxothiazol und Moa-Stilben nicht mehr in der Lage, an den Komplex III der mitochondrialen Atmungskette zu binden. Zur Bestimmung optimaler Selektionsbedingungen von mitochondrialen Transformanten mit den Hemmstoffen Antimycin A, Myxothiazol und Moa-Stilben wurden Testreihen mit Tabakblättern, Tabakprotoplasten sowie Tabak- und Arabidopsis-Suspensionskulturen durchgeführt. Bei „particle-gun“-Transformationen von Tabakblättern mit der Transformationskassette pUMarg II wurden 23 Moa-Stilben-resistente Linien selektiert. Bei 18 Linien konnte eine Integration des mutierten cob-Fragmentes eindeutig per PCR und Sequenzierung nachgewiesen werden. Ob es sich dabei um eine mitochondriale Integration handelt, konnte nicht eindeutig belegt werden. In diesem Fall wäre jedoch zu klären, auf welchem Mechanismus der offensichtliche selektive Vorteil der mit dem Transformations-vektor transformierten Pflanzen beruht, da die Integration des cob-Gen-Fragmentes nur innerhalb des mitochondrialen Zielortes funktionell sein dürfte. Plastidentransformation- Bei dem plastidären Transformationsansatz dieser Arbeit wurden zwei Ziele verfolgt. Zum einen wurde der „feedback“-Regulationsmechanismus der DHDPS innerhalb des Aspartat-Stoffwechsels durch die Integration eines insensitiven dhdps-Gens in die Plastiden von Tabak und Kartoffel beeinträchtigt. Der Gehalt der essentiellen Aminosäure Lysin wird somit gesteigert. Zum anderen wurde das insensitive dhdps-Gen als Markergen genutzt, um eine Selektion von plastidären Transformanten auf dem Lysinanalogon AEC zu ermöglichen. Erhöhung des Lysingehaltes- Es konnten 34 Spectinomycin-resistente Tabakklone selektiert werden, die mit der Transformationskassette pHoDh2b transformiert wurden. Die molekularbiologische Charakterisierung der Transformanten konnte bei 8 Linien eindeutig eine korrekte plastidäre Integration der Transgene belegen. Biochemische Analysen zeigen eine bis zu 32-fache Erhöhung an freiem Lysin im Blattgewebe von plastidären Transformanten. Es war damit erstmals mit der Plastidentransformation möglich, regulatorisch die Lysinbiosynthese zu verändern. AEC als plastidärer Selektionsmarker- AEC (S-Aminoethyl-L-Cystein) konkurriert als Lysinanalogon mit Lysin um den Einbau in Polypeptide. Wird die AEC-Konzentration im pflanzlichen Gewebe im Vergleich mit Lysin zu hoch, kommt es zum Erliegen des Stoffwechsels und damit zum Absterben der Zelle. Durch die Überproduktion von Lysin in den Transformanten verschiebt sich das Verhältnis von AEC → Lysin. Es wird somit kompetitiv mehr Lysin in die Polypeptide eingebaut. Dies lässt sich als Selektionsvorteil nutzen. Mit dem „Markergen“ dhdps-r1 und AEC als selektivem Hemmstoff wurden insgesamt 49 Tabaklinien selektiert. Bei 3 selektierten Linien konnte eindeutig die korrekte plastidäre Intergration der Transformationskassette nachgewiesen werden. Es ist damit erstmals gelungen, einen antibiotikumfreien universell einsetzbaren plastidären Selektionsmarker zu etablieren. In weiteren Transformationsexperimenten konnten mit dem gleichen „Markergen“ plastidäre Tomatentransformanten identifiziert und charakterisiert werden (Diplomarbeit L. Schaeffer). Damit ist es bereits bei 2 Spezies gelungen, AEC als selektiven Hemmstoff einzusetzen. Bei weiterer Optimierung der Selektionsbedingungen dürfte es möglich sein, auch bei weiteren Pflanzenspezies diesen universellen Selektionsmarker zu etablieren.