Phenotype of the typical form of a species as it occurs in nature
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4 episodes with Wild type
3 episodes with Wild type
4 episodes with Wild type
2 episodes with Wild type
The statistics of Poisson distributions can seem complex at first but are simpler than you think, which is important to know given their relevance to digital PCR. In short, they dictate the confidence you can have in the absolute quantification provided by dPCR. Dr. Dave Bauer, Thermo Fisher Scientific's very own dPCR Product Applications Specialist and statistics whiz, joins us once again for this short-but-sweet episode that's a must hear for those working with rare, or low-abundance PCR targets. Dave and the hosts talk about applications like cancer research where these types of samples are common, and then get into the details of how the Applied Biosystems QuantStudio Absolute Q Digital PCR System works to provide elegantly simple technologies like false positive rejection, background subtraction, low dead volume microfluidic array plates, and a master mix with chamber loading dye. Join us to learn what each of these are all about and how they help to provide confidence and reliability in results that matter for your precious samples. Visit the Absolute Gene-ius pageto learn more about the guests, the hosts, and the Applied Biosystems QuantStudio Absolute Q Digital PCR System.
Darshan H. Brahmbhatt, Podcast Editor of JACC: Advances, discusses a recently published original research paper on DPD quantification correlates with extracellular volume and disease severity in wild-type transthyretin cardiac amyloidosis.
Welcome to the Reptiles With Podcast the Wild Type Podcast: @TheWildTypePodcast Lyssa's Lizards: @LyssasLizards Neptune the Chameleon: @NeptunetheChameleon Yoshii: @modernreptileshop Chuckey: @madbioreptiles Eric: @reptiverse Become a Member: https://www.youtube.com/channel/UCaQN1-ZcfQ6ulG2hE41lIKw/join MERCH: https://modernreptileshop.com/collections/reptile-merch-for-sale1 Links: https://www.youtube.com/channel/UCaQN1-ZcfQ6ulG2hE41lIKw/join The Reptiles With Podcast covers Repti-Culture with a comedic twist. We aim to give different perspectives on all topics, even the controversial ones. --- Support this podcast: https://podcasters.spotify.com/pod/show/reptileswith/support
Darshan H. Brahmbhatt, Podcast Editor of JACC: Advances discusses a recently published original research paper on Age- and Sex-Related Differences in Patients with Wild-Type Transthyretin Amyloidosis
Dr Miller discusses findings from the SIENDO trial of selinexor maintenance therapy in patients with TP53 wild-type advanced endometrial cancer.
Lenz discusses the link between HER2 expression and responses with mCRC treatment regimens and how HER2 expression can guide treatment decision-making.
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CME credits: 1.00 Valid until: 23-04-2025 Claim your CME credit at https://reachmd.com/programs/cme/attr-amyloidosis-familial-vs-wild-type/24132/ Deepen your understanding of hereditary transthyretin amyloid polyneuropathy (ATTRv-PN) with this comprehensive CME series! Gain valuable insights from patient cases, expert lectures, and interviews. Explore the complexities of ATTRv-PN, differentiate familial vs wild-type forms, identify red flags for early detection, and review the latest diagnostic tools. Discover promising new therapies and what the future may hold for ATTRv-PN treatment. Join the experts and equip yourself to effectively manage this challenging disease and improve patient outcomes.
Designing a successful PCR assay is all about selecting the right primers to deliver the sensitivity and selectivity for which PCR is known for. But anyone that's designed an assay themselves will know that doing so successfully is a lot harder it sounds. We're joined by two PCR assay design pros for this episode. Kimi Soohoo Ong, and Dr. Rounak Feigelman, both from Thermo Fisher Scientific, shine a light on the many factors that must be considered to design a winning PCR assay. From the level of fragmentation of nucleic acids in the sample, to what other species' genomes that may be present in the sample, to what the sample matrix may contain, to the PCR master mix being used, if multiplexing is required, to what assay controls will be, and more! These two practiced bioinformaticians cover these challenges and then tell us how their team overcomes challenges to develop winning assays for both qPCR and dPCR applications. Our conversation uncovers the level of skill and artistry that goes into this craft. As always, you get to learn a bit more about our guests' backgrounds and career paths in the Cassie's Career Corner portion of the interview. They share how they both chose a bioinformatics path over wet lab work, while also acknowledging how important the wet lab work is to what they do. They also share some great advice and resources for anyone looking to explore a career in bioinformatics. Visit the Absolute Gene-ius page to learn more about the guests, the hosts, and the Applied Biosystems QuantStudio Absolute Q Digital PCR System.
Chris is the Director of Bryant Research and the Head of Policy at the Alternative Proteins Association. He is a social scientist and an expert on alternative protein markets and marketing. He has published several papers on consumer acceptance, policy, nutritional value, and other social dimensions of cultivated meat, plant-based meat, and fermentation-derived animal product alternatives. He has worked with alternative protein companies and non-profits, including THIS, Formo, Ivy Farm Technologies, Aleph Farms, Wild Type, ProVeg International, Mercy For Animals, and the Good Food Institute. In Sentientist Conversations we talk about the two most important questions: “what's real?” & “who matters?” Sentientism is "evidence, reason & compassion for all sentient beings." The video of our conversation is here on YouTube. We discuss: 00:00 Clips! 00:53 Welcome 02:55 Chris' Intro - Social science research and analysis helping those "trying to move our food system away from animals and towards alternatives" - "Industrial animal farming... one of the biggest utility sinks" 06:46 What's Real? - Growing up in a "nominally religious" household... "vaguely Christian" - Turning to #atheism and thinking "what is the answer then... what does matter?" - An interest in philosophy at university "realised that philosophy was the thing I had been talking about" - Going #vegetarian - #effectivealtruism "an incredibly powerful tool... applying empirical evidence to altruistic intentions... trying to come up with the best ways of doing good... given limited resources" - "I had a good go at #hedonism in the interim though" - Naturalistic #epistemology and "being willing to be wrong about things" - The dangers of unquestioned assumptions - Crash testing ideas that go against the mainstream (e.g. are "processed" foods really unhealthy?) - Choosing good sources, doing research and running experiments - How people form and update beliefs: cognitive dissonance, motivated reasoning, working backwards to justify what we want to believe or do (e.g. consuming animal products)... - Even before that: ignorance (not knowing) and neglect / avoidance (not thinking about it) "Most people have probably never thought about that question [e.g. what % of animals are factory farmed]". "It's important to reach those people [~50%?] with just the reality and the facts" - Strongly and weakly-held beliefs - "For some people that's progress... at least they're having to do the mental gymnastics whereas before it had just never occurred to them" - "The fact that so few people have thought about it should be some kind of weird hope for vegetarians and vegans" - Push [away from animal products - ethics, environment, health] and pull [towards the alternatives - cheap, healthy, appealing, available] factors. "Really for long time we didn't have very good pull factors... appealing alternatives to offer people." - Motivated reasoning: "If I lose this argument I'll have to go vegan and only eat beans... but if you can have burgers and nuggets and everything else... I'm not very motivated to try and defend this view any more" - Social norms & the expectations of others "Not just what I think of veg*ism but what do others think of it" ...and much more. Full show notes at Sentientism.info. Sentientism is “Evidence, reason & compassion for all sentient beings.” More at Sentientism.info. Join our "I'm a Sentientist" wall via this simple form. Everyone, Sentientist or not, is welcome in our groups. The biggest so far is here on FaceBook. Come join us there! --- Send in a voice message: https://podcasters.spotify.com/pod/show/sentientism/message
Enjoy this teaser of what you can expect while listening to The Wild Type Podcast. New episodes every Tuesday!
Polymerase chain reaction (PCR) was discovered in 1983 by Kary Mullis and Michael Smith, who were jointly awarded the Nobel Prize in Chemistry in 1993. Since then, PCR has been a cornerstone method that has been a pillar of discovery and applied science. The various types of PCR are sometimes confusing, and the relative pros and cons of each method are not always clear, which is why it's so great to have this episode's guest explain them all in a simple and clear-cut way. Dave Bauer, PhD, is an Application Scientist at Thermo Fisher Scientific that specializes in real time PCR (qPCR) and digital PCR (dPCR). He has an educational background in physics, mathematics, and biology, but what's more important is that Dave loves to help others learn and to break down a topic's complexities to make it more understandable and approachable. In this episode we hear Dave explain the difference between qPCR and dPCR, the importance of Poisson statistics to dPCR, dead volume, reaction chamber volume consistency, and more. We learn how qPCR and dPCR complement each other and how they relate to sequencing methods for applications like single nucleotide polymorphism (SNP) detection. As you've come to expect from Absolute Gene-ius, you also get a good sense of who Dave is and how he got to his current role. We learn about how he knew right away that academia wasn't for him, how he ended up unexpectedly working in forensics after his PhD, and how he eventually landed in his current Application Scientist role. Dave shares some great insights and advice, including how students should care less about their degree's name and more about what techniques they're learning and using in their studies. Visit the Absolute Gene-ius page to learn more about the guest, the hosts, and the Applied Biosystems QuantStudio Absolute Q Digital PCR System.This episode includes the following sound effects from freesound.org, licensed under CC BY-ND 4.0:“Sax Jazz,” by alonart“Balloon Pop / Christmas cracker / Confetti Cannon,” by Breviceps“Crowd Cheering,” by SoundsExciting
Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2023.07.31.551330v1?rss=1 Authors: Hodorovich, D. R., Harris, T. F., Burton, D. F., Neese, K. M., Bieler, R. A., Chudasama, V., Marsden, K. C. Abstract: The larval zebrafish is a highly versatile model across research disciplines, and the expanding use of behavioral analysis has contributed to many advances in neuro-psychiatric, developmental, and toxicological studies, often through large-scale chemical and genetic screens. In the absence of standardized approaches to larval zebrafish behavior analysis, however, it is critical to understand the impact on behavior of experimental variables such as the size of testing arenas and the choice of embryo medium. Using a custom-built, modular high-throughput testing system, we examined the effects of 4 testing arena sizes and 11 types of embryo media on conserved sensorimotor behaviors in zebrafish larvae. Our data show that testing arena size impacts acoustic startle sensitivity and kinematics as well as spontaneous locomotion and thigmotaxis, with fish tested in larger arenas displaying reduced startle sensitivity and increased locomotion. We also find that embryo media can dramatically affect startle sensitivity, kinematics, habituation, and pre-pulse inhibition, as well as spontaneous swimming, turning, and overall activity. Common media components such as methylene blue and high calcium concentration consistently reduced startle sensitivity and locomotion. To further address how the choice of embryo medium can impact phenotype expression in zebrafish models of disease, we reared chd7 mutant larvae, a model of CHARGE syndrome with previously characterized morphological and behavioral phenotypes, in 5 different types of media and observed impacts on all phenotypes. By defining the effects of these key extrinsic factors on larval zebrafish behavior, these data can help researchers select the most appropriate conditions for their specific research questions, particularly for genetic and chemical screens. Copy rights belong to original authors. Visit the link for more info Podcast created by Paper Player, LLC
Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2023.05.02.539100v1?rss=1 Authors: De La Torre, A., Huynh, T. N., Chang, C. C., Pooler, D. B., Ness, D., Lewis, L., Pannem, S., Feng, Y., Samkoe, K. S., Hickey, W. F., Chang, T. Y. Abstract: Copy rights belong to original authors. Visit the link for more info Podcast created by Paper Player, LLC
In this episode of the podcast, the Water Colors team is looking outside the "designer" clownfish box and exploring the natural biodiversity in clownfishes (Amphiprion spp.). Each species has a unique phylogenetic history, ecology, and behavioral repertoire. Or, said another way, this natural biodiversity means that there is a clownfish species that is perfect for almost every marine aquarium in the world. If you have wild-type clownfish in your home aquarium, please share your photos on the Water Colors Aquarium Gallery Podcast Listeners Facebook group! Correction/Addendum: - ORA was originally selling a Amphiprion melanopus clade clownfish from Fiji as Amphiprion rubrocinctus. In 2008, this population was described as Amphiprion barberi. ORA managed to confirm this identification with DNA testing. This story is a great example of why collection locations matter so much. In this case, the name changed, but it was still the "red clownfish from Fiji". Having a known collection location allowed professional breeders to correctly reidentify the fish they were selling. Fishes Mentioned in this Episode: - Percula clownfish (Amphiprion percula) - Ocellaris clownfish (Amphiprion ocellaris) - White-stripe maroon clownfish (Amphiprion biaculeatus) - Gold-stripe maroon clownfish (Amphiprion epigrammata) - Cinnamon clownfish (Amphiprion melanopus) - Tomato clownfish (Amphiprion frenatus) - Wide-Band clownfish (Amphiprion latezonatus) - Barrier Reef clownfish (Amphiprion akindynos) - Mcculloch's clownfish (Amphiprion mccullochi) - Clark's clownfish (Amphiprion clarkii) - Saddleback clownfish (Amphiprion polymnus) - Sebae clownfish (Amphiprion sebae) - Three-banded clownfish (Amphiprion tricinctus) - Allard's clownfish (Amphiprion allardi) - Japanese Clark's clownfish (Amphiprion clarkii japonicus) - Black Clark's clownfish (Amphiprion clarkii milii) - Solomon Islands Clark's clownfish (Amphiprion clarkii papuensis) - Red Sea clownfish (Amphiprion bicinctus) - Chagos clownfish (Amphiprion chagosensis) - Mauritian clownfish (Amphiprion chrysogaster) - "Whitetail" Blue-stripe clownfish (Amphiprion chrysopterus) - Seychelles clownfish (Amphiprion fuscocaudatus) - Madagascar clownfish (Amphiprion latifasciatus) - Oman clownfish (Amphiprion omanensis) - Black-footed clownfish (Amphiprion nigripes) - Yellow tang (Zebrasoma flavescens) - "Blackfin" Blue-stripe clownfish (Amphiprion cf. chrysopterus "Blackfin") - White-bonnet clownfish (Amphiprion chrysopterus x A. sanderacinos "leucokranos") - Orange skunk clownfish (Amphiprion sandaracinos) - Mariana Blue-stripe clownfish (Amphiprion cf. chrysopterus "Mariana" - Vanuatu Blue-stripe clownfish (Amphiprion cf. chrysopterus "Vanuatu") - Polynesian Blue-stripe clownfish (Amphiprion cf. chrysopterus "Polynesia") - Barber's clownfish (Amphiprion barberi) - Fire clownfish (Amphiprion ephippium) - Ruby/Cinnamon clownfish (Amphiprion rubrocinctus) - Pacific skunk clownfish (Amphiprion pacificus) - Yellow skunk clownfish (Amphiprion akallopisos) - Pink skunk clownfish (Amphiprion perideraion) - Thielle's clownfish (Amphiprion ocellaris x A. sanderacinos "thiellei) - "Fiji Sunkist" pink skunk clownfish (Amphiprion cf. perideraion "Fiji") - African skunk clownfish (Amphiprion cf. akallopisos "Africa")
Plant-based meat alternatives are supposed to be better for the planet and they are supposed to be good for you. But as they have grown in popularity, there are claims from some quarters that there is something of a halo effect at play, and that some of these health and environmental claims do not quite stack up. Evidence is offered which mentions the nutritional shortcomings of plant-based processed foods, as well as the carbon footprint being far higher than vegetables or legumes. In the midst of a climate crisis, where greenwashing is commonplace, this stuff really matters and it can be hard to cut through the noise to get to the facts. Which is where today's guest on the Food Matters Live podcast, Chris Bryant comes in, he has reviewed a host of previous studies to see where we are up to. Dr Chris Bryant, Research Associate, University of Bath - Director, Bryant Research Chris Bryant PhD is a social scientist and an expert on alternative protein markets and marketing. He is a Research Associate at the University of Bath. He has published several papers on consumer acceptance of cultivated meat, plant-based meat, and fermentation-derived animal product alternatives. Through his company, Bryant Research Ltd, he works with alternative protein companies and non-profits, including Formo, Ivy Farm Technologies, Aleph Farms, Wild Type, and the Good Food Institute.
Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2023.01.12.523571v1?rss=1 Authors: Iezza, D., Predella, C., NI, K., Murray, J. W., Liu, H.-Y., Saqi, A., Glasser, S. W., Dorrello, N. V. Abstract: Childhood interstitial lung disease (chILD) secondary to pulmonary surfactant deficiency is a devastating chronic lung disease in children. Clinical presentation includes mild to severe respiratory failure and fibrosis. There is no specific treatment, except lung transplantation, which is hampered by a severe shortage of donor organs, especially for young patients. Repair of lungs with chILD represents a longstanding therapeutic challenge but cellular therapy is a promising strategy. As surfactant is produced by alveolar type II epithelial (ATII) cells, engraftment with normal or gene-corrected ATII cells might provide an avenue to cure. Here we used a chILD disease-like model, Sftpc-/- mice, to provide proof-of-principle for this approach. Sftpc-/- mice developed chronic interstitial lung disease with age and were hypersensitive to bleomycin. We could engraft wild-type ATII cells after low dose bleomycin conditioning. Transplanted ATII cells produced mature SPC and attenuated bleomycin-induced lung injury up to four months post-transplant. This study demonstrates that partial replacement of mutant ATII cells can promote lung repair in a mouse model of chILD. Copy rights belong to original authors. Visit the link for more info Podcast created by Paper Player, LLC
Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2022.10.17.512455v1?rss=1 Authors: Yaguchi, K., Hagihara, M., Konno, A., Hirai, H., Yukinaga, H., Miyamichi, K. Abstract: Breastfeeding, which is essential for the survival of mammalian infants, is critically mediated by pulsatile secretion of the pituitary hormone oxytocin from the central oxytocin neurons located in the paraventricular and supraoptic hypothalamic nuclei of mothers. Despite its importance, the molecular and neural circuit mechanisms of the milk ejection reflex remain poorly understood, in part because a mouse model to study lactation was only recently established. In our previous study, we successfully introduced fiber photometry-based chronic imaging of the pulsatile activities of oxytocin neurons during lactation. However, the necessity of Cre recombinase-based double knock-in mice substantially compromised the use of various Cre-dependent neuroscience toolkits. To overcome this obstacle, we developed a simple Cre-free method for monitoring oxytocin neurons by an adeno-associated virus vector driving GCaMP6s under a 2.6 kb mouse oxytocin mini-promoter. Using this method, we monitored calcium ion transients of oxytocin neurons in the paraventricular nucleus in wild-type C57BL/6N and ICR mothers without genetic crossing. By combining this method with video recordings of mothers and pups, we found that the pulsatile activities of oxytocin neurons require physical mother-pup contact for the milk ejection reflex. Notably, the intensities and/or frequencies of photometric signals were dynamically modulated by mother-pup reunions after isolation and during natural weaning stages. Collectively, the present study illuminates the temporal dynamics of pulsatile activities of oxytocin neurons in wild-type mice and provides a tool to characterize maternal oxytocin functions. Copy rights belong to original authors. Visit the link for more info Podcast created by Paper Player, LLC
As we continue to empty the oceans, our species' demand for fish only seems to increase. But what if we could eat all the bluefin tuna and salmon we wanted without having to harm fish and other aquatic animals? That's the vision that companies like Wildtype are working toward. Founded in 2016, this cultivated fish startup has raised $120 million so far and now has 60 employees who are growing real fish meat without the fish. I've enjoyed their product now twice, both pre-pandemic and recently, and enjoyed it both times. Wild Type salmon that I recently enjoyed while in their San Francisco HQ. Today, Wildtype is building serious cultivation capacity to help turn the tide for the oceans, and in this episode, we discuss the company's origins, the role bird poop played, and what the company's up to today. We even discuss the co-founders' first company idea which they abandoned in favor of Wildtype: a redesigned Neti pot that would've been called The Schnozel. (They never trademarked this, so maybe one day you'll be able to buy that Schnozel of your dreams.) In addition to chatting about whether it's faster and cheaper to grow fish cells compared to mammalian and avian cells, co-founders Justin and Arye open up about what impact it's had on their personal lives to have gone from normal jobs to running their own company. We also talk about the perennial question in this industry: If the FDA gave them approval today, how soon would it be before we see Wildtype fish on restaurant menus? Discussed in this episode Our past episode with BlueNalu New Harvest founder Jason Matheny recently became CEO of the Rand Corporation The 2018 book Clean Meat (still highly relevant!) Bored Cow's great chocolate milk made with Perfect Day's animal-free whey protein More about Justin Kolbeck and Aryé Elfenbein Justin Kolbeck is co-founder and CEO of Wildtype, which is on a mission to create the cleanest, most sustainable seafood on the planet. Before Wildtype, he spent nearly five years as a consultant at Strategy& (not a typo!) helping companies develop and launch products, grow into new markets, and operate efficiently. Justin started his career as a Foreign Service Officer, serving in Pakistan, Afghanistan, Australia, and Washington DC. He is a graduate of the Yale School of Management, L'Institut d'Études Politiques de Paris, and UC Berkeley. Aryé Elfenbein is co-founder of Wildtype, where he directs the company's scientific research. Aryé completed his MD and PhD at Dartmouth and Kyoto University; he completed his clinical training in internal medicine and cardiology at Yale. Prior to Wildtype, he completed a fellowship in regenerative cardiovascular medicine research at the Gladstone Institutes / UCSF. He currently practices cardiology in the critical care setting.
Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2022.09.21.508917v1?rss=1 Authors: Ravi, S., Criado-Marrero, M., Barroso, D., Braga, I. M., Bolen, M., Rubinovich, U., Hery, G. P., Grudny, M. M., Koren, J., Prokop, S., Febo, M., Abisambra, J. F. Abstract: Background: Repetitive mild traumatic brain injury (rmTBI) is a leading and severe threat to cognition that often goes undiagnosed. A major challenge in developing diagnostics and treatments for the consequences of rmTBI is the fundamental knowledge gaps that explain how TBI promotes brain dysfunction. It is both critical and urgent to understand the neuropathological and functional consequences of rmTBI to develop effective therapeutic strategies. In this study, we defined the extent of altered brain functional connectivity (FC) and expression of neuropathological markers after rmTBI. Methods: We performed two rmTBI (2x 0.6 J impacts 24 h apart) in male and female C57BL/6J wild-type (WT) (~2.5-3mo) mice using CHIMERA or sham procedures. At 5-6 days post-injury (dpi), we measured changes in brain volume and FC using T2-weighted images, resting-state functional MRI (rsfMRI) and graph theory analyses. We used diffusion tensor imaging (DTI) to assess microstructural changes in white matter tracts. In addition, at 7dpi, we measured changes in Iba1 and GFAP in specific brain regions to determine the extent of gliosis. The expression of disease-associated protein markers in grey and white matter (WM) regions were evaluated using the NanoString-GeoMx digital spatial protein profiling spatial profiling (DSP) platform. Results: The rsfMRI data revealed aberrant changes in connectivity such as node clustering coefficient, global and local efficiency, participation coefficient, eigenvector centrality, and betweenness centrality in thalamus and other key brain regions that process visual, auditory, and somatosensory information. Using DTI, we found that fractional anisotropy (FA) and axial diffusivity in the optic tract was significantly decreased. Also, mean, radial, and axial diffusivity (L1) were significantly increased in the hippocampus. DSP revealed that phospho-serine 199 tau (pS199) as well as glial markers such as GFAP, cathepsin-D, and Iba1 were significantly increased in the optic tract. In thalamic nuclei, the neuroinflammatory marker GPNMB was increased significantly, and the cell proliferation marker Ki-67 was significantly decreased in the rmTBI group. Our data suggest that rmTBI significantly alters brain functional connectivity and causes a profound inflammatory response in gray matter regions, beyond chronic white matter damage. Copy rights belong to original authors. Visit the link for more info Podcast created by PaperPlayer
Description: Dr. Maya Graham interviews Dr. David Solomon about his and his team's recent manuscript entitled: "Prospective genomically-guided identification of "early/evolving" and "undersampled" IDH-wildtype glioblastoma leads to improved clinical outcomes", published online in Neuro-Oncology in April 2022. Read Article
In Episode 10 of the Plants are People too Podcast we speak with Prairie Moon Nursery native seed and plant sales consultant, Rob Kretz. In this episode, Rob shares his knowledge about some methods of prairie/grassland restoration, direct sowing methods, sourcing native seeds from around the region, how to improve the wildlife value of your lawn, vacant lot or 10+ acre property. Towards the end of the episode, Rob discusses some strategies for long term management with both fire and mechanical methods. We also touch on Veronicastrum virginicum (Culver's Root) and delve into some hypothetical discussions on epigenetics, climate change and possible implications of introducing genetically different plants into regions where native populations of the same species might be rare, threatened or endangered. Enjoy!Support the show (https://www.patreon.com/user?u=65399395)
On this episode of Riot Act Reviews we are looking at the 6th studio album from alt-rock legends Failure, Wild Type Droid. Since reforming in 2015 Failure have been one of the exceptions to the rule that your post-reformation material can't live up to your classics, putting out two albums of exceptional quality. This time around the band have hinted that they will be putting the space and sci-fi themes they have been working with since 1996's legendary Fantastic Planet album to bed. So does Wild Type Droid close the book on this chapter of Failure's career with a happy ending? Or are they going to crash this ship entirely?... you probably already know what we think, but have a listen anyway would you. This podcast uses the following third-party services for analysis: Chartable - https://chartable.com/privacy
Today, we talk to Proctor. Proctor's story also started when he was 18-19 years old. Much like the same with Pierre, Proctor also experienced pain in his perineum... and in his testicles. He also went to different doctors, was prescribed with pain medications, and still no explanation with how the pain is occurring. Then he had robotic radical prostatectomy after his Prostate Cancer was picked up by a diligent GP. All of these brought Proctor to Dr. Jo and Melissa - and the rest is history. :) This episode will not only share Proctor's journey, discuss 'wild type erections' but will also show you the importance of having a very diligent GP. Enjoy listening! ---------- If you feel The Penis Project is valuable to you then, please review and subscribe as this will ensure more people get to hear what we have to say. ---------- Websites: https://thepenisproject.org/ https://rshealth.com.au/ http://www.menshealthphysiotherapy.com.au/ http://prost.com.au/ https://www.theyogavine.com.au Facebook: https://www.facebook.com/Restorativeshealthclinic Music David Mercy https://open.spotify.com/artist/1HbvnltKu4XbWTmk0kpVB9?si=D1xP5dDVQK-zzNU3rViRWg Producer Thomas Evans: The SOTA Process https://www.instagram.com/thesotaprocess/ https://open.spotify.com/show/4Jf2IYXRlgfsiqNARsY8fi
Commentary by Dr. Olivier Florian Clerc
On the next episode of the Eight One Sixty, it's all about new music! We just finished an amazing four-part series called Sound Minds all July, and during that time we received SO MANY new music submissions. Chris will be sharing that music with you for the next four weeks of August.This week, we'll hear new stuff from 11 KC area bands/musicians:Hipshot Killer, just released “Guns and Gas” EP on July 30Hembree just released a new single on July 20Sisterbot just released a new album, “Truth House,” on July 10Tre Mutava just released a new album, “The Movies,” on July 30Kadesh Flow just released a new album, “Room Service 2,” on July 19Sunshine Lombré just released a new album, "Fading Away,” in JulyBaby and the Brain just released another new single on July 30The Way Way Back just released their new album on July 23Use Your Atlas are releasing “Rise and Fall” EP on Aug. 1The Life I Love released new singles back in AprilRachel Mallin and The Wild Type just released “Thank You Stranger” EP on July 23
On the next episode of the Eight One Sixty, it's all about new music! We just finished an amazing four-part series called Sound Minds all July, and during that time we received SO MANY new music submissions. Chris will be sharing that music with you for the next four weeks of August.This week, we'll hear new stuff from 11 KC area bands/musicians:Hipshot Killer, just released “Guns and Gas” EP on July 30Hembree just released a new single on July 20Sisterbot just released a new album, “Truth House,” on July 10Tre Mutava just released a new album, “The Movies,” on July 30Kadesh Flow just released a new album, “Room Service 2,” on July 19Sunshine Lombré just released a new album, "Fading Away,” in JulyBaby and the Brain just released another new single on July 30The Way Way Back just released their new album on July 23Use Your Atlas are releasing “Rise and Fall” EP on Aug. 1The Life I Love released new singles back in AprilRachel Mallin and The Wild Type just released “Thank You Stranger” EP on July 23
This week on the Streetwise podcast we discuss Dog Version 2.0, jam to The Wild Type's track "Closet Anthem," chat with Brian Daldorph (author of Words is a Powerful Thing), and rock a bonus Nick's music corner with Ricky Roosevelt & Alccalh's song "No Tint."Streetwise is hosted by Brock Wilbur, editor in chief of The Pitch. Subscribe on iTunes or Spotify. Subscribe to the Streetwise newsletter, a weekly meditation on Kansas City news and events.Jason Klamm read our magazine story. Thanks to Jason Beers for our theme song, “One Kicker.” Our editor is Terence Wiggins.
The Consumer VC: Venture Capital I B2C Startups I Commerce | Early-Stage Investing
My guest today is Justin Kolbeck, CEO and co-founder of Wild Type. Wild Type is on a mission to create the cleanest most sustainable seafood on the planet, starting with salmon. This is the first founder of a cell based food company I've had on the show and as someone who didn't know much about cell base food or commercial fishing, this was quite eye opening for me. We talk about the current state of the ocean, the supply chain for fish, the process for producing cell based food and their approach to quick feedback loops in trials.Some of the questions I ask Justin:When were you first introduced to cell based seafood and meat?What was your attraction to entrepreneurship? What was that decision making process like? Was it gradual?What was the insight that led you to founding Wildtype foods?Why is this product so essential for the world? What's the current state of commercial fishing?Why do you believe you were in a unique position to found this company?When founding WildType Foods, what was the first step? How did you approach making a cell based salmon product?Why did you decide to start with salmon?What's your go-to-market strategy?How do you imagine your ideal consumer?Is there a difference in taste?How did you approach investors and raise capital?What's one thing you would change about fundraising or venture capital?What's the best piece of advice that you've received?What's one piece of advice for founders?
Jacalyn Warshauer is the Founder of Wild Type Learning. She shares the many benefits of allowing children to connect with nature and how we can nurture this connection in meaningful ways. She shares her fascinating journey towards founding Wild Type Acres, the land that she and her husband purchased and built a log cabin home on where they now live with their 2 young boys. Jacalyn reminds us about the importance of slowing down, existing as we are are, and reconnecting to ourselves. Listen in now and feel grounded and inspired!
FDA Drug Information Soundcast in Clinical Oncology (D.I.S.C.O.)
Listen to a soundcast of April 6th & 7th FDA approvals of Erbitux (cetuximab) for K-Ras wild type, EGFR-expressing colorectal cancer or squamous cell carcinoma of the head and neck and Trodelvy (sacituzumab govitecan) for unresectable locally advanced or metastatic triple negative breast cancer.
Welcome to the premier of Storytime with Larry Anthony. Today, I am sitting down with singer-songwriter//producer Mark Rose (Spitalfield) to chat about the new music business and his evolution as an artist. As the main course, we explore his influences and compare his two full length albums: Wonderful Trouble and The Wild Type.Be sure to check out Mark Rose anywhere you stream music or support him through purchase via https://markrosemusic.bandcamp.com/As a thank you tuning in to the debut, we are running this episode AD FREE. Video coming soon via Patreon. Thanks for watching!
SHR # 2594 :: Unique Safety Issues Associated With Virus-vectored Vaccines: Potential For Theoretical Consequences of Recombination with Wild Type Virus Strains - Keith Bell - The COVID-19 vaccine will be a virus-vector vaccine. In 2003 and 2013, the World Health Organization convened informal consultations on characterization and quality aspects of vaccines based on live virus vectors. In the resulting reports, one of several issues raised for future study was the potential for recombination of virus-vectored vaccines with wild type pathogenic virus strains. Our discussion today assesses this issue formulated by the Brighton Collaboration. Study Discussed On Today's Show https://www.sciencedirect.com/science/article/pii/S0264410X16302250?fbclid=IwAR1gsVw1VoikQyfbdHk4qfB8cSSeWbFi44WvSevNqni6ioHqPldb6hWXUxg
SHR # 2594 :: Unique Safety Issues Associated With Virus-vectored Vaccines: Potential For Theoretical Consequences of Recombination with Wild Type Virus Strains - Keith Bell - The COVID-19 vaccine will be a virus-vector vaccine. In 2003 and 2013, the World Health Organization convened informal consultations on characterization and quality aspects of vaccines based on live virus vectors. In the resulting reports, one of several issues raised for future study was the potential for recombination of virus-vectored vaccines with wild type pathogenic virus strains. Our discussion today assesses this issue formulated by the Brighton Collaboration. Study Discussed On Today's Show https://www.sciencedirect.com/science/article/pii/S0264410X16302250?fbclid=IwAR1gsVw1VoikQyfbdHk4qfB8cSSeWbFi44WvSevNqni6ioHqPldb6hWXUxg
Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2020.09.16.300020v1?rss=1 Authors: Sagi, M., Kurmanbayeva, A., Bekturova, A., Soltabayeva, A., Srivastava, S., Oshanova, D., Nurbekova, Z. Abstract: The role of the cytosolic O-acetylserine-(thiol) lyase A (OASTLA), chloroplastic OASTLB and mitochondrion OASTLC in plant resistance/sensitivity to selenate was studied in Arabidopsis plants. Impairment in OASTLA and B resulted in reduced biomass, chlorophyll and soluble protein levels compared with impaired OASTL C and Wild-Type treated with selenate. The lower organic-Se and protein-Se levels followed by decreased organic-S, S in proteins and total glutathione in oastlA and oastlB compared to Wild-Type and oastlC are indicative that Se accumulation is not the main cause for the stress symptoms, but rather the interference of Se with the S-reduction pathway. The increase in sulfite oxidase, adenosine 5'-phosphosulfate reductase, sulfite reductase and OASTL activity levels, followed by enhanced sulfite and sulfide, indicate a futile anabolic S-starvation response to selenate-induced organic-S catabolism in oastlA and oastlB compared to Wild-Type and oastlC. Additionally, the catabolic pathway of L-cysteine degradation was enhanced by selenate, and similar to L-cysteine producing activity, oastlA and B exhibited a significant decrease in L-cysteine desulfhydrase (DES) activity, compared with WT, indicating a major role of OASTLs in L-cysteine degradation. This notion was further evidenced by sulfide dependent DES in-gel activity, immunoblotting, immunoprecipitation with specific antibodies and identification of unique peptides in activity bands generated by OASTLA, B and C. Similar responses of the OASTLs in Seleno-Cysteine degradation was demonstrated in selenate stressed plants. Notably, no L-cysteine and L-Seleno-Cysteine DES activity bands but those related to OASTLs were evident. These results indicate the significance of OASTLs in degrading L-cysteine and L-SelenoCysteine in Arabidopsis. Copy rights belong to original authors. Visit the link for more info
CardioNerd (Amit Goyal) join Washington University in St. Louis cardiology fellows (Adam Lick, Manny Rivera Maza, and Sam Lindner) for some amazing local St. Louis craft brews! They discuss a fascinating case of wild-type aTTR cardiac amyloid. Prior to meeting up with the group, Amit bumps into Rachita Navara: a Wash U #FIT, aspiring electrophysiologist, & a rock star of the band "The Pacemakers" (be sure to check out their performance at the end of the episode!) who shares thoughts about the program and her cutting edge contributions to the field of EP. Dr. Katie Zhang provides the E-CPR and program director Dr. Andy Kates provides a message for applicants. Episode notes were developed by Johns Hopkins internal medicine resident Colin Blumenthal with mentorship from University of Maryland cardiology fellow Karan Desai. Jump to: Patient summary - Case figures & media - Case teaching - References - Production team Episode graphic by Dr. Carine Hamo The CardioNerds Cardiology Case Reports series shines light on the hidden curriculum of medical storytelling. We learn together while discussing fascinating cases in this fun, engaging, and educational format. Each episode ends with an “Expert CardioNerd Perspectives & Review” (E-CPR) for a nuanced teaching from a content expert. We truly believe that hearing about a patient is the singular theme that unifies everyone at every level, from the student to the professor emeritus. We are teaming up with the ACC FIT Section to use the #CNCR episodes to showcase CV education across the country in the era of virtual recruitment. As part of the recruitment series, each episode features fellows from a given program discussing and teaching about an interesting case as well as sharing what makes their hearts flutter about their fellowship training. The case discussion is followed by both an E-CPR segment and a message from the program director. CardioNerds Case Reports PageCardioNerds Episode PageCardioNerds AcademySubscribe to our newsletter- The HeartbeatSupport our educational mission by becoming a Patron!Cardiology Programs Twitter Group created by Dr. Nosheen Reza Patient Summary A man in his early 70s, with a history of hypertension, a bicuspid aortic valve, chronic kidney disease and carpal tunnel syndrome presents with two weeks of worsening dyspnea on exertion. At baseline, he is an avid cyclist and noticed he can now only bike ½ mile when before he could bike extended distances. In addition, he noted abdominal swelling and palpitations. Vitals signs showed mild tachycardia, irregularly irregular rhythm, and no clear evidence of volume overload. Labs demonstrated acute on chronic kidney disease, an elevated NT-proBNP and elevated troponin. ECG demonstrated atrial flutter with variable conduction block. TTE demonstrated marked concentric left ventricular hypertrophy with preserved ejection fraction, biatrial enlargement, reduced global longitudinal strain with apical sparing, and bicuspid aortic valve with moderate aortic stenosis. Further diagnostics revealed normal serum kappa/lambda light chains and PYP scan was positive. Patient underwent EMB which demonstrated ATTR amyloid deposition and genetic screening did not show mutations commonly associated with hereditary ATTR. Case Media ABCD A. CXR: Stable mild enlargement of the cardiac silhouette; mildly tortuous aorta. Lung fields are clear, no pneumonia, pleural effusions, or pneumothorax.B. AV continuous wave DopplerC. Strain ImageD. Tc-99 PYP Scan TTE 1 TTE 4 TTE 2 TTE 5 TTE 3 TC-99 PYP Scan Strain video 1 Strain video 2 Strain video 3 Episode Schematics & Teaching Created by Dr. Karan DesaiCreated by Dr. Carine Hamo (updated 9.2020)Click to enlarge! The CardioNerds 5! – 5 major takeaways from the #CNCR case Cardiac amyloidosis can have a range of cardiac and extra-cardiac findings as amyloid fibrils can d...
Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2020.08.18.254649v1?rss=1 Authors: Audrain, M., Haure-Mirande, J.-V., Mleczko, J., Wang, M., Griffin, J. K., Fraser, P., Zhang, B., Gandy, S., Ehrlich, M. E. Abstract: Microglial TYROBP (also known as DAP12) has been identified by computational transcriptomics as a network hub and driver in late-onset sporadic Alzheimer's disease (AD) and as an important regulator of the microglial environmental sensing function. TYROBP is the transmembrane adaptor of AD-related receptors TREM2 and CR3, but importantly, TYROBP interacts with many other receptors, and little is known about its roles in microglial action and/or in the pathogenesis of AD. Herein, using dual RNA in situ hybridization and immunohistochemistry, we demonstrate that endogenous Tyrobp transcription is increased specifically in recruited microglia in the brains of wild-type and AD-related mouse models. To determine whether chronically elevated TYROBP might modify microglial phenotype and/or progression of AD pathogenesis, we generated a novel transgenic mouse overexpressing TYROBP in microglia. TYROBP-overexpressing mice were crossed with either APP/PSEN1 or MAPTP301S mice, resulting in a decrease of the amyloid burden in the former and an increase of TAU phosphorylation in the latter. Apolipoprotein E (Apoe) transcription was upregulated in MAPTP301S mice overexpressing TYROBP and transcription of genes previously associated with Apoe, including Axl, Ccl2, Tgfb and Il6, was altered in both APP/PSEN1 and MAPTP301S mice overexpressing TYROBP. Lastly, Tyrobp and Apoe mRNAs were clearly increased in Trem2-null mice in microglia recruited around a cortical stab injury or amyloid-beta deposits. Conversely, microglial Apoe mRNA level was dramatically diminished when Tyrobp was absent. Our results provide compelling evidence that TYROBP-APOE signaling in the microglial sensome does not require TREM2. We propose that activation of a TREM2-independent TYROBP-APOE signaling could be an early or even initiating step in the transformation of microglia from the homeostatic phenotype to the Disease-Associated Microglia (DAM) phenotype. Copy rights belong to original authors. Visit the link for more info
Essa semana, no Intech: No Brasil: - Avus, startup que oferece um “cartão de benefícios” em saúde a baixo custo (foco: iFood e afins); Ao redor do mundo: - Edtech Classpert captando R$5MM; - Microsoft e a aquisição do TikTok US. Além disso, compromisso de zerar suas emissões de carbono até 2030. Livro Satya Nadella: Hit Refresh. - Kuleana com plant-based atum, além de Wild Type, Finless Foods e Shiok Meats criando salmão, camarão e outras coisas que eu nunca vou comer! Mercado gde! - Trickle apostando em microlearning com um app baseado em pílulas de conteúdos curados. - GreyNoise captando cerca de R$25MM para filtrar sinal x ruído em sistemas de segurança. - Filter off: speed dating por video! O Intech, podcast da Labenu, é um trechinho da aula semanal que estudantes da Labenu têm para se atualizar em notícias de tecnologias e negócios que surgiram na semana. Formamos devs em uma experiência de 1000h em desenvolvimento de software, em um modelo que reduz as barreiras de acesso ao mercado de tecnologia . Indique para os amigos! @labenu_ no Instagram ou www.labenu.com.br
Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2020.08.05.237172v1?rss=1 Authors: Paschini, M., Reyes, C. M., Gillespie, A. E., Lewis, K. A., Glustrom, L. W., Sharpee, T. O., Wuttke, D. S., Lundblad, V. Abstract: Telomeres present unique challenges for genomes with linear chromosomes, including the inability of the semi-conservative DNA replication machinery to fully duplicate the ends of linear molecules. This is solved in virtually all eukaryotes by the enzyme telomerase, through the addition of telomeric repeats onto chromosome ends. It is widely assumed that the primary site of action for telomerase is the single-stranded G-rich overhang at the ends of chromosomes, formed after DNA replication is complete. We show here that the preferred substrate for telomerase in wild type yeast is instead a collapsed fork generated during replication of duplex telomeric DNA. Furthermore, newly collapsed forks are extensively elongated by telomerase by as much as ~200 nucleotides in a single cell division, indicating that a major source of newly synthesized telomeric repeats in wild type cells occurs at collapsed forks. Fork collapse and the subsequent response by telomerase are coordinated by the dual activities of a telomere-dedicated RPA-like complex, which facilitates replication of duplex telomeric DNA and also recruits telomerase to the fork, thereby ensuring a high probability of re-elongation if DNA replication fails. We further show that the ability of telomerase to elongate newly collapsed forks is dependent on the Rad51 protein, indicating that telomerase activity in response to fork collapse proceeds through a regulatory pathway distinct from how telomerase engages fully replicated chromosome termini. We propose a new model in which spontaneous replication fork collapse and the subsequent response by telomerase is a major determinant of telomere length homeostasis. Copy rights belong to original authors. Visit the link for more info
Dr. W. Michael Korn, Chief Medical Officer at Caris Life Sciences® and Dr. Philip A. Philip, who serves as the Kathryn Cramer, MD, Endowed Chair in Cancer Research and an investigator on this study discuss the data presentation from the American Society of Clinical Oncology, (ASCO20) Virtual Scientific Program, Abstract 4629/Poster 237: Enrichment of alterations in targetable molecular pathways are enriched in KRAS wild-type (WT) pancreatic cancer (PC).
Teri Cochrane is an nutritional counsellor and integrative practitioner that has developed her own methodology for helping people with their nutrition and health issues. The health of Teri's children led her to seek solutions to their health crises. They both recovered from life-threatening illnesses when she discerned the root cause of their imbalances and when they changed the way they ate to support their unique genetic blueprint. Teri applies this thinking and approach to each of her clients. As a result, Teri and her team have transformed thousands of people to better their health. Teri's approach is so effective that she coined it as the “The Cochrane Method” in honour of her children. This method is also applied in her latest book The Wildatarian Diet: Living as Nature Intended. This book has pioneering research that teaches us that there is no one health food for everyone and how pathogens, stress, and our environment can influence our genes and therefore our health. The diet is completely customized to your individual Wild Type. In this episode we discuss: Teri's story of how her sick children took her from being a risk manager to practicing nutrition full time and saving the life of her son. The dangers of mould, oxidative stress and amyloid plaques. What should and shouldn't be on your plate. Killer chickens and killer corn. How energy and vibration effect the foods you consume. The Wildatarian approach and Teri's book. Top tips to put into practice straight away to improve your nutrition and overall health. Plus much much more. Access Teri's FREE PDF (usually $7.95) here: https://tericochrane.com/wildly-easy-7-day-meal-prep-guide/kinetikfitness/ Teri's Instagram: https://www.instagram.com/tericochranebeyondnutrition/ Teri's website: https://tericochrane.com/ --- Send in a voice message: https://anchor.fm/thekinetikfitnessshow/message Support this podcast: https://anchor.fm/thekinetikfitnessshow/support
It’s no secret our oceans are in trouble. Climate change and bad policy continue to contribute to major shifts in one of the most complex ecosystems on the planet. Among the most pressing challenges are ocean acidification (the ocean today is, on average, 25% more acidic than in pre-industrial times), overfishing (a staggering 93% of all fisheries are being fished at or beyond capacity, while demand for seafood continues to rise the world over) and plastic pollution (about half of which is a direct result of fishing and seafood consumption; more than 640,000 tons of “ghost gear” — discarded fishing equipment — ends up in oceans every year). And while efforts to address these issues have ramped up in recent years, they have often focused on the wrong things. In this episode of Feeding 10 Billion, Varun and Ramya sit down with Jen Lamy, manager of the Sustainable Seafood Initiative at the Good Food Institute, to discuss the problems facing our oceans, the complex and opaque seafood supply chain and why plant-based and cultivated seafood are the best way forward. SHOW NOTES: What is plant-based meat and seafood? Plant-based products are direct replacements for animal-based products, such as plant-based meat, seafood, eggs and dairy. These include products that use the biomimicry approach to replicate the taste and texture of meat, as well as plant-forward products (like jackfruit, seitan, tofu and tempeh) that serve as functional meat replacements. Source What is cultivated meat and seafood? Cultivated meat (often referred to as cell-based meat or clean meat) is genuine animal meat that can replicate the sensory and nutritional profile of conventionally produced meat because it’s comprised of the same cell types and arranged in the same three-dimensional structure as animal tissue. It isn’t imitation or synthetic meat; it’s actual meat that is grown from cells outside of an animal. Source Dr Mark Post’s Ted Talk at TedX Haarlem in 2013: Meet the new meat What is aquaculture? The term aquaculture broadly refers to the cultivation of aquatic organisms in controlled aquatic environments for any commercial, recreational or public purpose. The breeding, rearing and harvesting of plants and animals takes place in all types of water environments including ponds, rivers, lakes, the ocean and man-made “closed” systems on land. Source What is ocean acidification? Ocean acidification refers to a reduction in the pH of the ocean over an extended period of time, caused primarily by uptake of carbon dioxide (CO2) from the atmosphere. Source Organizations, Institutes, Cultivated, and Plant-Based Companies: The Good Food Institute’s Sustainable Seafood Initiative Developing and commercializing plant-based and cell-based seafood is the most tractable path for ensuring seafood sustainability and improving the health of our oceans. GFI’s Sustainable Seafood Initiative provides targeted research and support with a laser focus on accelerating this sector. FAO The Food and Agriculture Organization (FAO) is a specialized agency of the United Nations that leads international efforts to tackle food insecurity, world hunger, and food sustainability. IPCC The Intergovernmental Panel on Climate Change (IPCC) is the United Nations body for assessing the science related to climate change. Shiok Meats Shiok in Singapore and Malay slang means fantastic and delicious. Shiok Meats is the first cell-based clean meat company in Singapore and South-East Asia. Their mission is to bring delicious, clean and healthy seafood like crustacean meats (including shrimp, crab and lobster) by harvesting meat from cells instead of animals. Their meats are animal-, health- and environment-friendly with the same taste, texture, more nutrients and no cruelty. Avant Meats Avant Meats Company Limited is a start-up that focuses on R&D of cell-based agriculture. They strive to bring down the product costs of cell-based meat by researches (sic) and collaboration with companies of tangential technologies. BlueNalu, Inc BlueNalu's mission is to be the global leader in cellular aquaculture™, providing consumers with great tasting, healthy, safe, and trusted seafood products that support the sustainability and diversity of our oceans. Their aim is to supplement current industry practice, in which fish are farmed or wild-caught in our ocean and seas, by producing real seafood products directly from fish cells, that are as delicious and nutritious as products that have been grown conventionally, in a way that is healthy for people, humane for animals, and sustainable for our planet. WIld Type Wild Type’s mission is to create the cleanest, most sustainable seafood on the planet. They use cellular agriculture technologies to address the most pressing challenges of our generation: climate change, food security, and health. Finless Food Finless Foods is a food startup working toward a world where everyone has access to healthy, delectable seafood, without the environmental devastation or the health hazards of traditional fishing and aquatic farming. Starting with bluefin tuna, they use cutting-edge cellular-agriculture technologies to grow marine-animal cells, creating fish and seafood products enjoyed around the world. Good Catch The Good Catch team are passionate culinary rebels with a cause—seafood without sacrifice. They bring you the rich flavors and flaky textures of fine seafood—from fish-free tuna and burgers to crab-free cakes—made with nutritious, sustainable ingredients. Their mission is to “preserve the ocean’s natural resources while introducing awesomely delicious “seafood” choices that benefit you and the world. Nothing fishy about it.” Ocean Hugger Ocean Hugger Foods offers healthy, delicious and sustainable plant-based alternatives to your favorite seafood proteins. Created by one of America's top chefs, Certified Master Chef James Corwell. Their flagship product, Ahimi™, is the world's first plant-based alternative to raw tuna, perfect for use in sushi, poke, tartare, ceviche and more. ADDITIONAL READING: Find more information about Maharashtra's ban on single-use plastics here Read about Indian Prime Minister Narandra Modi’s pledge to ban single-use plastic here Read why the push to ban plastic straws — when fishing accounts for 46% of all ocean plastic — is well intentioned but misguided *here** More information about the dire state of our oceans and why plant-based and cultivated seafood are the best way forward can be found here Read more about Canada’s seafood traceability issue here Read IPCC’s “The Ocean and Cryosphere in a Changing Climate” report here More information about the rising levels of microplastic in our oceans can be found here Read more about the Van Cleve Seafood Company’s first plant-based seafood launch, Wild.Skinny.Clean, here Here’s an article about Northern Harvest’s salmon die-off and cleanup efforts.
Evan Holod is the CEO of Michel Et Augustin, a cookie company located in France, but Evan is bringing it to the states with a colorful brand and personality Evan is a funny guy with a candid sense of humor but has a lot of wisdom to share about marketing brands like Bazooka Joe, Ring Pop, and Coca-Cola. Michel et Augustine is recently pushing into retail launching in many different grocery stores around the United States. We talk about how to reset your career trajectory, as Evan worked in finance for 7 years before getting a degree in marketing and starting as a marketing assistant for food, then worked his way up to CEO for food. We also talk about marketing tactics. From packaging to convincing people to go with your crazy ideas. About Evan Evan Holod is the CEO (and Chief Troublemaker) at Michel et Augustin, a leading French manufacturer founded in 2004 and known in Europe for their unique approach to everything. A lifelong eater, Evan spends his days with the unenviable task of introducing French pastry know-how to unsuspecting Americans. Whether ideating the next great Franco-American food innovation, securing a distribution deal, or participating in another grueling cookie taste test, Evan and his team of trublions (French for ‘troublemaker’) will stop at nothing to make sure that Americans of all ages have access to delicious, honestly indulgent cookies like their bestselling Cookie Squares (buttery shortbread cookie squares filled with a variety of different chocolate ganache recipes). Prior to his current adventure, Evan was the Brand Director for Coca-Cola in the U.S., responsible for developing and implementing the U.S. brand strategy, targeted marketing communications and advertising, and creating and executing programs like the “Share a Coke” campaign. Evan spent his early years in marketing at glacéau, working on the team that helped drive the growth of smartwater. Evan holds multiple degrees in unrelated and peculiar subjects, with a B.A. from Duke University in History and a Masters in Sports Marketing from NYU. Married for over 10 years and with 2 often lovable children, Evan and his family moved back to NY in 2018 and now reside in Brooklyn, NY. Sponsor The Cultured Meat Symposium is taking place in San Francisco on November 14-15th. Join experts from science, food, and tech industries to discuss the future of food. Join speakers from Aleph Farms, Finless Foods, Memphis Meats, Wild Type, IntegriCulture, VOW, the American Meat Science Association and more. Use coupon code MYFOODJOBROCKS20 for 20% off General Admission tickets. Or use this link here I’ll be there with a mobile podcast studio. See you there! Show Notes First sentences: I’m the chief troublemaker. We’re a cookie company that helps people brighten their day. Michel En Augustin – people who traded their suits for pastry hats. Our employees have to pass the French pastry exam We have one card for everyone. We have 11 of us in the United States so we all have it on the card. Michele and Augustin have been around for 15 years in France, but it’s so different in the United States Danone buyout Coca Cola Who was a mutual friend who introduced you to Augustine?: A French friend in Coca Cola How did you get to where you are today?: I used to work in investing but hated it after 7 years. I wanted to work in sports but my investor friend said to work in consumer goods. I got my degree in marketing and then got a job as a marketing assistant and rose to Coca-Cola and ended up having sports as my portfolio because Coca-Cola is the best brand in the world My role in coca-cola: my job is to recruit a new generation of coke drinkers There are tons of marketing divisions in coca-cola from stores Mexican Coke How do you convince people?: People need to trust you, but you have to bring people along in the beginning. People like to be involved Where do you manufacture?: In Europe, sometimes we have to ship by air to the US Where are you available?: Some SF stores, available at Safeway, Target, Shoprite, Fairway, 20% Coupon if you message Evan on LinkedIn We were slow on retail. We mostly focused on food service and front-end We decided to focus on multipacks. We had to do a lot of different ways to repackage. We had to do some of our manufacturing in the US such as packing products What is one thing in the food industry you’d like to know more about?: The science and the technical side. It’s a language I don’t speak or understand. I don’t like having to tap out of the conversation How do you learn?: I don’t read anymore. I’ve reached to a point where it’s more curated. Tom Goodwin, Mark Rittztons Where can we find you?: First Thrusday at 98 4th Street Open House 7-8pm at Brooklyn Facebook Page: Michel et Augustin
Joe Heitzeberg is the CEO and Co-Founder of Crowd Cow, an e-commerce site that sells premium meat. And when I say premium, I mean meat straight from the farm, or Japan! We all hear about the innovations of alternative meat, plant-based or cell-based meat all over this podcast so I was delighted to interview a new perspective in the way we view protein, the fact that you can not only find out where your steak comes from, but how it was raised, what breed it is, and it’s delivered straight to your door. As long as it reduces the system in how we farm animals now, I’m all for it. Learn about how Joe pivoted the business from actually crowdfunding a cow, to building out a way to sell Wagyu beef to his customers. And most importantly, learn how innovation doesn’t have to be a new method of making meat, but maybe just showcasing old methods using modern technology. Sponsor The Cultured Meat Symposium is taking place in San Francisco on November 14-15th. Join experts from science, food, and tech industries to discuss the future of food. Join speakers from Aleph Farms, Finless Foods, Memphis Meats, Wild Type, IntegriCulture, VOW, the American Meat Science Association and more. Use coupon code MYFOODJOBROCKS20 for 20% off General Admission tickets. Or use this link here I’ll be there with a mobile podcast studio. See you there! Show Notes In a sentence or less: We help consumers know the source of their meat Why online? We can bring a richer experience than in the grocery store Why is the meat better?: The type of meat and what they eat is much higher quality. They’re well-taken care of Why did you start Crowd Cow?: When an Entrepreneur in Residence, one of my friends was bragging about getting a cow. I went to the farm with him and saw just how different it is to get a cow We first started with crowdfunding one cow, it was fun and engaging but in reality, it didn’t work Wagyu/Kobe Beef – called Koroge Washu, genetically, the marbling is different. We had to get it. We called the slaughterhouse in Japanese and asked them to sell us meat. I had to visit them to get their business. Why Does Your Food Job Rock?: I love learning the process and the consumer feedback What do you think about plant-based and cell-based?: Imitation is flattery. However, I agree that the industrialization of meat is an issue that they are trying to solve. We also have a way to fix it. People want an alternative to factory farming What’s the biggest challenge right now?: Reach and brand recognition Favorite resource: Badass: Making Users Awesome Any advice for entrepreneurs?: Keep one foot in front of the other, you’re farther than you think Also, be around people with founder empathy Until you’ve convinced someone to do things for free with no resources or brand, you’re not an entrepreneur Where can we find you for advice?: joe@crowdcow.com What’s your favorite beef right now?: We just launched an Iwate Wagyu beef. Even the less-marbled cuts taste amazing Are you sharing the efficiencies of your model verses factory farming?: Yes How can you get away from the commodity system?: The people who sell commodity meat don’t know or care about how special the beef is, we do
This episode is with Akshita Iyers, CEO and Founder of Inirv, a smart kitchen tech company whose first product is a kitchen knob that you can control on your phone! Akshita Iyer started this company with her husband when her mother forgot to turn off the stovetop, and burnt down her house. At the time, Akshita and her husband were in the medical industry and dropped everything to build a company that would have more impact. I had a fun time with this live interview as we chat about building a smart kitchen device. From going on Shark Tank to asking amazing people for advice, we dive in on how to accelerate this process and you will be surprised how the process is very similar to a food business. This is one of two interviews I did live at SKS in Seattle last month. It was a super fun event, and I’ll go a little bit into it at the beginning of the episode. Learn the building blocks of building something as complex as a smart kitchen device. About Akshita Akshita is the co-founder and CEO of Inirv, an IoT company using connected devices to bring safety and simplicity to the kitchen. Akshita graduated from Duke University with a neuroscience degree and worked in the Duke Health System before becoming an entrepreneur. After her mom accidentally left the stove on and started a fire, Akshita founded Inirv to bring connectivity to the kitchen in the fastest, most affordable way. Inirv is reimagining the kitchen and transforming the way we cook at home. Sponsor The Cultured Meat Symposium is taking place in San Francisco on November 14-15th. Join experts from science, food, and tech industries to discuss the future of food. Join speakers from Aleph Farms, Finless Foods, Memphis Meats, Wild Type, IntegriCulture, VOW, the American Meat Science Association and more. Use coupon code MYFOODJOBROCKS20 for 20% off General Admission tickets. Or use this link here I’ll be there with a mobile podcast studio. See you there! Show Notes Adam Monologue Smart Kitchen Summit SKS Rakuten Optimism 2019 Cal Fussman's podcast Big Question Tim Ferriss Show Cal Fussman James Altucher Cal Fussman Marie Kondo Daymond John Faux Fried Chicken on Shark Tank Wild Earth on Shark Tank Intrapreneur The Power Of Broke Paul Shapiro Smart Kitchen Summit I make products that help you cook safely at home At Inirv, we build connective devices to make things simple What’s your background?: I graduated in Duke and focused more on neuroscience. I always wanted to be an entrepreneur but after the accident, we thought this would be great to help a lot of people How did you think of this idea?: How do we control the appliance instead of solving the reaction? The threshold for a kitchen knob is very low and we’ve tested 100s of stoves to make sure we are compatible. We shipped product, 7000 people on the waitlist How can we get on your waitlist?: Just our website Inirv.com Shark Tank -How can you get on the show?: You can audition but we had someone reach out to us to be on the show. It took 9 months of diligence before we went to LA. You can be cut any time. 30000 people pitch, 150 pitch, 50 get on air. The first 30 seconds are scripted, once that’s over, it’s a complete free for all. There’s a 2 hour pitch. What have you found cool about SKS?: Great way to network Chefling What are your thoughts on kitchen devices?: We’re on the cusp of it. The tip of the iceberg Why do you love what you do?: I love building things. I didn’t have access to any resources but the passion helped built it up What are some challenges and solutions?: We were able to talk to the founders of other smart kitchen companies and it helped us a ton. How do you get through the noise?: Make your note personal. Do your research What’s your favorite resources?: How I built this by Guy Raz A book: Predictably Irrational. I actually took his class What is one piece of advice to start a tech company?: Surround yourself with amazing people. My family helped me a lot. We almost ran out money to manufacture it but we did it. Number one reason startups fail because founders get up We interviewed 100+ hardware founders about manufacturing akshita@inirv.com reach out on my website and linkedin What’s next?: Scaling production. Work with other players and appliance manufacturers. We’re exploring a lot of options
In this episode, we are joined by Teri Cochrane. Teri Cochrane is an internationally known health expert specializing in complex and chronic conditions as well as optimizing elite performers. She recently authored Amazon #1 newly released book titled The Wildatarian Diet: Living as Nature Intended. Teri started as a risk manager by trade but became a risk manager for her children's health when their health started declining while following the traditional path of medicine. Their health conditions led her to seek solutions to their health crises. Both of her children recovered from life-threatening illnesses when she discerned the root cause of their imbalances and when they changed the way they ate to support their unique genetic blueprint. She applies this thinking and approach to each of her clients as well, resulting to thousands of people being transformed to better their health. Her approach is coined as the “The Cochrane Method”. This method is also applied in her latest book The Wildatarian Diet: Living as Nature Intended. This book has pioneering research that teaches us that there is no one health food for everyone and how pathogens, stress, and our environment can influence our genes and therefore our health. The diet is completely customized to your individual Wild Type. Teri Cochrane tells listeners: “There's no one health food for everyone and there's no one supplement for everyone. We are all different.” IN THIS EPISODE, YOU WILL LEARN: What is Bioindividuality The contribution of genes in diseases and illnesses Factors that influence our genetics Link between the food we eat, our microbiology and the illnesses today Factors that mess up our gut health Connect with Teri Cochrane: Follow Teri Cochrane on Facebook, LinkedIn, Instagram. Follow Teri Cochrane on website About Everyday Wellness Podcast Everyday Wellness is not just another health podcast. Your co-hosts, Cynthia Thurlow (nurse practitioner and functional nutritionist) and Dr. Kelly Donahue (clinical health psychologist and nutritional therapy consultant) have over 25 years of combined experience in medicine, psychology, and wellness. Our mission is to bring you the best, science-backed yet practical information to improve your physical and mental wellness every day. We are busy mom-preneurs and know how important your time is. We have designed this podcast to be short in time and big on impact. We interview a variety of guests in the field of health and wellness, and we discuss important issues and provide practical strategies that you can use in your real life.
Although obesity is a major risk factor for diabetes, certain environmental agents, such as arsenic, also appear to contribute to the disease. There is evidence that an individual’s risk of arsenic-related disease depends on how efficiently he or she metabolizes arsenic. But what if that individual is both obese and exposed to arsenic? In this podcast, guest Mirek Stýblo discusses his recent work exploring the role that diet plays not just in arsenic metabolism but also in the diabetogenic effects of arsenic. Visit the podcast webpage to download the full transcript of this podcast.
In the final episode of Season 2, Liz and Ivan talk with Jeff Long, a professor at the University of California, Los Angeles (UCLA). He is the vice chair of the Molecular, Cellular and Developmental Biology department and co-director of the Cell and Developmental Biology graduate program. Jeff has worked on embryogenesis and development in Arabidopsis for most of his career. He earned his Ph.D. at the University of Wisconsin and completed a postdoc at the California Institute of Technology. In 2003, he joined the Salk Institute for Biological studies before moving to UCLA in 2012. Like episode S1.5, which came out between Seasons 1 and 2, in this episode we do not discuss one of our guests’ papers (although we do recommend you look up Jeff’s many impactful publications). Instead, we talk about a recent publication in Nature Biotechnology regarding graduate student mental health. Over 2000 graduate students from many countries and fields beyond plant biology were surveyed, providing astonishing evidence for a mental health crisis in graduate education. The authors report 41% of students scoring high for anxiety issues and 48% scoring high for depression. While these data suffer from responder bias, clearly there is a higher prevalence of mental health issues among graduate students than the general population. Postdocs most likely share this trend. In this study, many students indicate that they feel undervalued and that the Principal Investigators (PIs) in their labs were not providing adequate mentorship, support, or assets to further their career. We explore some of the reasons students might be feeling this way and discuss ways to provide support and mitigate these issues for students. We talk frankly about some of our own experiences and why it is important to think not only about what are we saying, but also how are we being heard. In this season of Taproot, and especially in this episode, we want people who are facing struggles in their careers to know that they aren’t alone and having trouble isn’t unusual. This is the wild type phenotype for scientists, especially while in training! If you are experiencing depression, anxiety, or another mental health issue, please don’t wait for a crisis! Right now, you can reach out to others for help and together we can break down the stigma associated with these struggles. SHOW NOTES Paper: https://www.mcdb.ucla.edu/faculty/jeffalong Evans, T. M., Bira, L., Gastelum, J. B., Weiss, L. T., & Vanderford, N. L. (2018). Evidence for a mental health crisis in graduate education. Nature biotechnology, 36(3), 282. Levecque, K., Anseel, F., De Beuckelaer, A., Van der Heyden, J., & Gisle, L. (2017). Work organization and mental health problems in Ph.D. students. Research Policy, 46(4), 868-879. Ivan Baxter’s tweet regarding this topic; https://twitter.com/BaxterTwi/status/986730085418962944 Find us on Twitter: @ehaswell @baxtertwi @taprootpodcast
We're hanging out with the guys from Momma's Boy, hear a new single from them. Also, they’re busy guys, one of them recently announced he’s starting a record label called Manor Records and another is currently interning at Ink Magazine. We also hear brand new music from Rachel Mallin and The Wild Type, The MGDs, Hot Suede, Rubeo, Hembree and Joseph Warren & the Wanderlust Revival.
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To get live links to the music we play and resources we offer, visit This show includes the following songs: Bishon - Dr. Jeckyll & Mrs. Hyde The Angie Haze Project - We Will Lead Rosemary Gallagher - Maybe Andrea McArdle - A Husband Is Patricia Luce Chapman - King-size Canyon, Queen-size Bed Ilene Angel - The High Road Rachel Mallin & The Wild Type - Dropout For Music Biz Resources Visit Visit our Sponsor: Get music by Shantell Ogden at: Visit our Sponsor: Get music by Far West Folk Alliance at:
To get live links to the music we play and resources we offer, visit This show includes the following songs: Bishon - Dr. Jeckyll & Mrs. Hyde The Angie Haze Project - We Will Lead Rosemary Gallagher - Maybe Andrea McArdle - A Husband Is Patricia Luce Chapman - King-size Canyon, Queen-size Bed Ilene Angel - The High Road Rachel Mallin & The Wild Type - Dropout For Music Biz Resources Visit Visit our Sponsor: Get music by Shantell Ogden at: Visit our Sponsor: Get music by Far West Folk Alliance at:
To get live links to the music we play and resources we offer, visit This show includes the following songs: Bishon - Dr. Jeckyll & Mrs. Hyde The Angie Haze Project - We Will Lead Rosemary Gallagher - Maybe Andrea McArdle - A Husband Is Patricia Luce Chapman - King-size Canyon, Queen-size Bed Ilene Angel - The High Road Rachel Mallin & The Wild Type - Dropout For Music Biz Resources Visit Visit our Sponsor: Get music by Shantell Ogden at: Visit our Sponsor: Get music by Far West Folk Alliance at:
To get live links to the music we play and resources we offer, visit This show includes the following songs: Judy Klass - Hazy WIth A Chance of Rain Charlotte Berg - Under the Same Stars Morgan Myles - What You Do To Me Cathy DeWitt - Everybody's Somebody's Child Almost Awake - Skeleton S Two Feat. Judy K - Greg's Theme Rachel Mallin & The Wild Type - White Girls For Music Biz Resources Visit Visit our Sponsor: Get music by Andrea Speaks at:
To get live links to the music we play and resources we offer, visit This show includes the following songs: Judy Klass - Hazy WIth A Chance of Rain Charlotte Berg - Under the Same Stars Morgan Myles - What You Do To Me Cathy DeWitt - Everybody's Somebody's Child Almost Awake - Skeleton S Two Feat. Judy K - Greg's Theme Rachel Mallin & The Wild Type - White Girls For Music Biz Resources Visit Visit our Sponsor: Get music by Andrea Speaks at:
To get live links to the music we play and resources we offer, visit This show includes the following songs: Judy Klass - Hazy WIth A Chance of Rain Charlotte Berg - Under the Same Stars Morgan Myles - What You Do To Me Cathy DeWitt - Everybody's Somebody's Child Almost Awake - Skeleton S Two Feat. Judy K - Greg's Theme Rachel Mallin & The Wild Type - White Girls For Music Biz Resources Visit Visit our Sponsor: Get music by Andrea Speaks at:
The guys have friends Rachel Mallin and the Wild Type in to talk about SXSW and they play groovy acoustic tracks (including Razorback)! Facebook Link for Rachel Mallin: https://www.facebook.com/RachMalisSass Podcast File
I sat down with Jesse Bartmess of Sinple & Rachel Mallin & The Wild Type. We talked mainly about the passing of David Bowie among other things.
For patients with wild type EGFR, meaning there is no EGFR mutation, drugs like Tarceva (erlotinib) can have a small benefit, but Dr. Joan Schiller wants research to do better. February 2014
For patients with wild type EGFR, meaning there is no EGFR mutation, drugs like Tarceva (erlotinib) can have a small benefit, but Dr. Joan Schiller wants research to do better. February 2014
For patients with wild type EGFR, meaning there is no EGFR mutation, drugs like Tarceva (erlotinib) can have a small benefit, but Dr. Joan Schiller wants research to do better. February 2014
Eighty percent of lung cancer patients have wild type EGFR, meaning there is no EGFR mutation. Dr. Joan Schiller of UT Southwestern Medical Center discusses a new drug in development that may help those patients. February 2014
Eighty percent of lung cancer patients have wild type EGFR, meaning there is no EGFR mutation. Dr. Joan Schiller of UT Southwestern Medical Center discusses a new drug in development that may help those patients. February 2014
Eighty percent of lung cancer patients have wild type EGFR, meaning there is no EGFR mutation. Dr. Joan Schiller of UT Southwestern Medical Center discusses a new drug in development that may help those patients. February 2014
When it comes to EGFR and lung cancer, is a mutation good or bad? Dr. Joel Neal of Stanford University Medical Center explains why non-mutated genes are called "wild type." February 2014.
When it comes to EGFR and lung cancer, is a mutation good or bad? Dr. Joel Neal of Stanford University Medical Center explains why non-mutated genes are called "wild type." February 2014.
When it comes to EGFR and lung cancer, is a mutation good or bad? Dr. Joel Neal of Stanford University Medical Center explains why non-mutated genes are called "wild type." February 2014.
In this podcast, I will discuss first-line usage of anti-EGFR therapies in metastatic colorectal cancer in the context of the PEAK trial findings with a focus on the emerging data surrounding RAS oncogene mutations in treatment selection.
John Scarbrough of Gecko Boa Reptiles joins us for a very special episode all about wild type or sub species of the common Leopard Gecko. These are sought after world wide and John is one of the few breeders that has all of them. He may be the only person that has every individual sub species here in the USA at the time of this broadcast. We will hear about his experiences with these amazing and integral parts of the gecko hobby. Don't miss this Gecko Nation Radio exclusive interview. Airing on Sunday 2/16/14 at 8:00 PM Eastern. Please check out the Gecko Nation Radio facebook page!
Dr. Mark Socinski from University of Pittsburgh summarizes and critiques the TAILOR trial of Taxotere (docetaxel) vs. Tarceva (erlotinib) in EGFR wild type advanced NSCLC.
Dr. Mark Socinski from University of Pittsburgh summarizes and critiques the TAILOR trial of Taxotere (docetaxel) vs. Tarceva (erlotinib) in EGFR wild type advanced NSCLC.
Medizinische Fakultät - Digitale Hochschulschriften der LMU - Teil 14/19
Wed, 16 May 2012 12:00:00 +0100 https://edoc.ub.uni-muenchen.de/14404/ https://edoc.ub.uni-muenchen.de/14404/1/Radic_Tanja.pdf Radic, Tanja ddc:610, ddc:600, Med
Tierärztliche Fakultät - Digitale Hochschulschriften der LMU - Teil 05/07
Bovine herpesvirus type 1 (BoHV-1), an alphaherpesvirus, is a major pathogen of cattle causing different syndromes such as infectious bovine rhinotracheitis (IBR), infectious pustular vulvovaginitis (IPV) and infectious balanoposthitis (IBP). BoHV-1 control programs have been initiated in several European countries including Germany. One of the major components is the vaccination with inactivated or attenuated glycoprotein E (gE)-deleted live marker vaccines. The aim of this study was the development of a triplex real-time polymerase chain reaction (qPCR) assay for the sensitive, specific and reliable BoHV-1 detection. A BoHV-1-specific glycoprotein D (gD) assay was combined with a gE-specific qPCR system for differentiation between wild-type strains and gE-negative vaccine virus strains. Finally, an internal control based on amplification of the bovine beta-actin gene was introduced to verify efficient DNA extraction and PCR amplification. The analytical sensitivity of the triplex BoHV-1 qPCR enables the detection of 10 genome copies per reaction. Furthermore, the sensitivity of the newly developed qPCR assay was compared to an OIE-validated qPCR and the “gold standard” method of virus isolation in cell culture using 10-fold dilution series of BoHV-1 in extended semen as well as in cell culture medium. For all preparations, the tested qPCR assays showed comparable results and the sensitivity of the triplex qPCR was equal or even greater than that of virus isolation. A broad spectrum of reference strains and field isolates was detected reliably. The specificity of the test was confirmed using nasal swabs, semen and different organ materials of BoHV-1-negative cattle. Bovine herpesviruses type 2, 4 and 5 and further ruminant herpesviruses, namely bubaline herpesvirus (BuHV-1), caprine herpesvirus type 1 and cervine herpesvirus type 1 and 2 (CvHV-1, -2) were tested as well. The close genetic and serological relationship between these viruses combined with their ability to infect bovines may interfere with diagnostics resulting in false-positive results. All non-BoHV-1 herpesviruses were negative in the gD-specific assay, while BuHV-1, CvHV-1 and -2 were tested positive by the gE-specific qPCR. Consequently, the triplex qPCR offers for the first time the possibility to detect some related herpesviruses and distinguish them from BoHV-1 in addition to the simultaneous differentiation of BoHV-1 wild-type and gE-deleted vaccine strains.
John V. Heymach, MD, PhD, MD Anderson, Houston, Texas
Background: The atheroprotective effects of systemic delivery of either apolipoprotein A-I (wtApoA-I) or the naturally occurring mutant ApoA-I Milano (ApoA-I(M)) have been established in animal and human trials, but direct comparison studies evaluating the phenotype of ApoA-I or ApoAI-Milano knock-in mice or bone marrow transplantated animals with selectively ApoA-I or ApoAI-Milano transduced macrophages give conflicting results regarding the superior performance of either one. We therefore sought to compare the two forms of apoA-I using liver-directed somatic gene transfer in hypercholesterinemic mice - a model which is most adequately mimicking the clinical setting. Methods and results: Vectors based on AAV serotype 8 (AAV2.8) encoding wtApoA-I, ApoA-I(M) or green fluorescent protein (GFP) as control were constructed. LDL receptor deficient mice were fed a Western Diet. After 8 weeks the AAV vectors were injected, and 6 weeks later atherosclerotic lesion size was determined by aortic en face analysis. Expression of wtApoA-I reduced progression of atherosclerosis by 32% compared with control (p = 0.02) and of ApoA-I(M) by 24% (p = 0.04). There was no significant difference between the two forms of ApoA-I in inhibiting atherosclerosis progression. Conclusion: Liver-directed AAV2.8-mediated gene transfer of wtApoA-I and ApoA-I(M) each significantly reduced atherosclerosis progression to a similar extent.
Fri, 1 Jan 1971 12:00:00 +0100 https://epub.ub.uni-muenchen.de/7179/1/7179.pdf Pfaller, A.; Massinger, P.; Neupert, Walter
Modified live virus vaccines are one of the best ways to prevent and control PRRSV today, but PRRS cases continue to be a problem. When testing for PRRSV though, its hard to tell if a positive test is from the live vaccine or from the wild type. In this episode Dr. Guarav Rawal talks about his MLV vaccine specific PCR assay that can detect the presence of a vaccine virus in a sample and what that can mean for the swine industry. Dr. Gaurav Rawal is a Virology Lab Manager in the Department of Veterinary Diagnostic and Production Animal Medicine at Iowa State University (ISU). He received his DVM from Nepal in 2014 & MS from ISU in Veterinary Preventive Medicine with Drs Bailey Arruda & Daniel Linhares in 2019. His MS thesis was on understanding epidemiology, infection dynamics & cellular tropism of an emerging virus in the US swine industry i.e. porcine astrovirus type 3. He is currently working with Dr. Jianqiang Zhang on his Ph.D. with a focus on applied virology and PRRS vaccine immunology.
The Consumer VC: Venture Capital I B2C Startups I Commerce | Early-Stage Investing
My guest today is Sara Deshpande ( https://twitter.com/sara_desh ) , Partner at Maven Ventures ( https://www.mavenventures.com/ ). Maven Ventures is a consumer focused micro-fund that focuses on investing in seed stage companies. Some of their investments include Zoom, Wild Type, Angellist and Nuggs. What I most enjoyed about this conversation was looking at what types of businesses were being built on top of Zoom and the opportunities in telehealth. Without further ado, here's Sara. You can catch her on Twitter @sara_desh ( https://twitter.com/sara_desh ). Three books that have inspired Sara: The Alchemist ( https://www.amazon.com/gp/product/0062315005/ref=as_li_qf_asin_il_tl?creative=9325&creativeASIN=0062315005&ie=UTF8&linkCode=as2&linkId=355439103c8ff12c0bc489932836adac&tag=theconsumervc-20 ) by Paulo Coelho Shoe Dog ( https://www.amazon.com/gp/product/1501135929/ref=as_li_qf_asin_il_tl?creative=9325&creativeASIN=1501135929&ie=UTF8&linkCode=as2&linkId=2ff4d71035f0fe3c1ae6bb76703a55bf&tag=theconsumervc-20 ) by Phil Knight Maid ( https://www.amazon.com/gp/product/0316505099/ref=as_li_qf_asin_il_tl?creative=9325&creativeASIN=0316505099&ie=UTF8&linkCode=as2&linkId=0157a468a9378d3092a35fdbdf2d2484&tag=theconsumervc-20 ) by Stephanie Land Some of the questions I ask Sara: * You came from healthcare consulting at Deloitte. What was your attraction to venture capital, startups and innovation? * Tell us a bit about Maven. * What do you consider micro-VC? * Walk us through your due diligence process? * How do you access how big of a pain point a company is solving? * What are some of the opportunities that you're seeing telehealth? * Another area of focus are technology that serves families. What's an example of a company you're excited in this space or a problem that you are hoping to see solve? * Has COVID affected any of the themes you focus on? * What are some other trends that you're curious about? * What's one thing that you would change when it came to venture capital? * What's the best piece of advice that you've received? * What's one piece of advice that you have for founders?
Welcome to the Fresh Underground. We're going to take you to Nashville to kick out an old flame with some empowered twang and will close things out with an introspective and mellifluous cello piece from Belgium. This episode you'll meet Hannah Juanita, Lynne Hanson, Alper Tuzcu featuring Helena Beltrão, Elisete, Laini Colman, The Wild Type, Karen Meat, Easy Honey, The Maggie Pills, and Stijn Kuppens.
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