Podcasts about h2o2

Play Episode Listen Later Jul 28, 2023

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2023.07.27.550831v1?rss=1 Authors: Homolak, J., Varvaras, K., Sciacca, V., Perhoc, A. B., Virag, D., Knezovic, A., Barilar, J. O., Salkovic-Petrisic, M. Abstract: Introduction: Recent evidence suggests that the gut plays a vital role in the development and progression of Alzheimer's disease (AD) by triggering systemic inflammation and oxidative stress. The well-established rat model of AD, induced by intracerebroventricular administration of streptozotocin (STZ-icv), provides valuable insights into the GI implications of neurodegeneration. Notably, this model leads to pathophysiological changes in the gut, including redox dyshomeostasis, resulting from central neuropathology. Our study aimed to investigate the mechanisms underlying gut redox dyshomeostasis and assess the effects of D-galactose, which is known to benefit gut redox homeostasis and alleviate cognitive deficits in this model. Materials and Methods: Duodenal rings isolated from STZ-icv animals and control groups were subjected to a prooxidative environment using AAPH or H2O2 with or without D-galactose in oxygenated Krebs buffer ex vivo. Redox homeostasis was analyzed through protein microarrays and functional biochemical assays, alongside cell survival assessment. Structural equation modeling, univariate, and multivariate models were employed to evaluate the differential response of STZ-icv and control samples to the controlled prooxidative challenge. Results: STZ-icv samples showed suppressed expression of catalase and glutathione peroxidase 4 (GPX4) and increased baseline activity of enzymes involved in H2O2 and superoxide homeostasis. The altered redox homeostasis status was associated with an inability to respond to oxidative challenges and D-galactose. Conversely, the presence of D-galactose increased antioxidant capacity, enhanced catalase and peroxidase activity, and upregulated superoxide dismutases in the control samples. Conclusion: STZ-icv-induced gut dysfunction is characterized by a diminished ability of the redox regulatory system to maintain long-term protection through the transcription of antioxidant response genes, as well as compromised activation of enzymes responsible for immediate antioxidant defense. D-galactose can exert beneficial effects on gut redox homeostasis under physiological conditions. Copy rights belong to original authors. Visit the link for more info Podcast created by Paper Player, LLC

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Primary human lung fibroblasts exhibit trigger- but not disease-specific cellular senescence and impair alveolar epithelial cell progenitor function

Play Episode Listen Later Jul 26, 2023

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2023.07.24.550385v1?rss=1 Authors: Bramey, N., Melo-Narvaez, M. C., See, F., Ballester-Llobell, B., Steinchen, C., Jain, E., Hafner, K., Yildirim, A. O., Koenigshoff, M., Lehmann, M. Abstract: Aging is the main risk factor for chronic lung diseases including idiopathic pulmonary fibrosis (IPF) and chronic obstructive pulmonary disease (COPD). Accordingly, hallmarks of aging such as cellular senescence are increased in different cell types such as fibroblasts in the lungs of patients. However, whether the senescent phenotype of fibroblasts derived from IPF or COPD differs is still unknown. Therefore, we characterized senescence at baseline and after exposure to disease-relevant insults (H2O2, bleomycin, and TGF-{beta}1) in primary human lung fibroblasts (phLF) from control donors, IPF, and COPD patients. We found that phLF from different disease origins have a low baseline senescence. Moreover, senescence trigger and not disease origin defines their senescence phenotype. Finally, senescent fibroblasts interfere with the stem cell capacity of alveolar progenitors in vitro. Copy rights belong to original authors. Visit the link for more info Podcast created by Paper Player, LLC

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Long-term memory formation depends on an astrocyte-to-neuron H2O2 signaling

Play Episode Listen Later Jul 12, 2023

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2023.07.11.548505v1?rss=1 Authors: Preat, T., Rabah, Y., Pasquer, L., Sagar, N., Placais, P.-Y. Abstract: Astrocytes interact with neurons during cognitive processes. In particular, astrocytes help neurons to fight oxidative stress, a needed function since active neurons are prone to reactive oxygen species (ROS) damage due to their high mitochondrial activity and relatively poor antioxidant defenses. ROS also play major physiological functions, but it remains unknown how neuronal ROS signaling is activated during memory formation and if astrocytes play a role in that process. We discovered in Drosophila an astrocyte-to-neuron H2O2 signaling cascade essential for long-term memory formation. Stimulation of astrocytes by acetylcholine induces an intracellular calcium increase that triggers the formation of extracellular O2- by astrocytic NADPH oxidase. Superoxide dismutase 3 secreted by astrocytes converts O2- into H2O2, which is imported into neurons of the olfactory memory center (the mushroom body), as revealed by in vivo H2O2 imaging using an ultrasensitive sensor. Importantly, SOD3 activity requires Cu2+, which we show is delivered by the neuronal Amyloid Precursor Protein. Furthermore, we found that human amyloid-beta peptide, involved in Alzheimer s disease, inhibits the astrocytic cholinergic receptor and hampers memory formation by preventing H2O2 import into neurons. These findings could have major implications for the understanding of Alzheimer s disease etiology, as soluble synaptic Abeta42 correlates better with the pattern of cognitive decline in AD than amyloid plaques, and since early pathology in cholinergic neurons of the basal forebrain predicts memory defects. Copy rights belong to original authors. Visit the link for more info Podcast created by Paper Player, LLC

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Demystifying H2O2 | Expert Series On-The-Go

Beyond Clean Podcast

Play Episode Listen Later May 28, 2023 2:15

On this week's edition of the Beyond Clean Expert Series On-The-Go, tune in for the conversation entitled, "Demystifying H2O2". To hear more from this expert, follow us on Facebook and Linkedin, or subscribe to the Beyond Clean mailing list by visiting: https://educate.beyondclean.net/cleanfreaks or text BEYONDCLEAN to 22828 Do you know a #SterileProcessing expert who you'd love to hear from on our series? Or do you have a question for one of our current experts? Contact us at any time via email at: info@beyondclean.net Until next time, keep fighting dirty! #SterileProcessing #BeyondClean #ExpertSeries #Podcast

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Direct quantification of chemogenetic H2O2 production in live human cells.

Play Episode Listen Later May 4, 2023

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2023.05.03.539306v1?rss=1 Authors: den Toom, W. T. F., van Soest, D. M. K., Polderman, P. E., van Triest, M. H., Bruurs, L. J. M., Burgering, B. M. T., De Henau, S., Dansen, T. B. Abstract: Reactive Oxygen Species (ROS) in the form of H2O2 can act both as physiological signaling molecules as well as damaging agents, depending on its concentration and localization. The downstream biological effects of H2O2 were often studied making use of exogenously added H2O2, generally as a bolus and at supraphysiological levels. But this does not mimic the continuous, low levels of intracellular H2O2 production by for instance mitochondrial respiration. The enzyme D-Amino Acid Oxidase (DAAO) catalyzes H2O2 formation using D-amino acids, which are absent from culture media, as a substrate. Ectopic expression of DAAO has recently been used in several studies to produce inducible and titratable intracellular H2O2. However, a method to directly quantify the amount of H2O2 produced by DAAO has been lacking, making it difficult to assess whether observed phenotypes are the result of physiological or artificially high levels of H2O2. Here we describe a simple assay to directly quantify DAAO activity by measuring the oxygen consumed during H2O2 production. The oxygen consumption rate of DAAO can directly be compared to the basal mitochondrial respiration in the same assay, allowing to estimate whether the ensuing level of H2O2 production is within the range of physiological mitochondrial ROS production. We show that the assay can also be used to select clones that express differently localized DAAO with the same absolute level of H2O2 production to be able to discriminate the effects of H2O2 production at different subcellular locations from differences in total oxidative burden. This method therefore greatly improves the interpretation and applicability of DAAO-based models, thereby moving the redox biology field forward. Copy rights belong to original authors. Visit the link for more info Podcast created by Paper Player, LLC

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Mitochondrial H2O2 release does not directly cause genomic DNA damage.

Play Episode Listen Later Mar 30, 2023

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2023.03.29.534749v1?rss=1 Authors: van Soest, D. M. K., Polderman, P. E., den Toom, W. T., Zwakenberg, S., De Henau, S., Burgering, B. M. T., Dansen, T. B. Abstract: Reactive Oxygen Species (ROS) derived from mitochondrial respiration are frequently cited as a major source of genomic DNA damage and subsequent mutations that contribute to cancer development and aging. However, experimental evidence showing that ROS released by mitochondrial can directly damage nuclear DNA under (patho)physiological conditions has been largely lacking. In this study we modeled the effects of mitochondrial H2O2 release and compared this to H2O2 production at the nucleosomes in an untransformed human cell line. We used a chemogenetic approach to produce localized H2O2 and combined it with a new method we developed to directly quantify the amount of H2O2 produced. This enabled us to precisely investigate to what extent DNA damage occurs downstream of near- and supraphysiological amounts of localized H2O2 generation. Nuclear H2O2 production gives rise to DNA strand breaks, subsequent activation of the DNA damage response, cell cycle arrest and eventually senescence. Release of H2O2 from mitochondria on the other hand shows none of these effects, even at levels that are orders of magnitude higher than what mitochondria normally produce. Artificially high levels of mitochondrial H2O2 release do result in DNA strand breaks, but in parallel invariably cause ferroptosis-mediated cell death, preventing propagation of DNA damage-induced mutations. This study shows that H2O2 released from mitochondria is unlikely to directly damage genomic DNA, limiting its contribution to oncogenic transformation and aging. Copy rights belong to original authors. Visit the link for more info Podcast created by Paper Player, LLC

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Ask DR ARDIS Live: 3/13/23 1:30pm PT, 4:30pm ET

Ba'al Busters Broadcast

Play Episode Listen Later Mar 15, 2023 104:32

If you want to try 35% H2O2, Save yourself over $13 from that company Dr Ardis mentioned, and look at this site, instead. I've seen it as low as $16.45 in the past. The outfit I displayed wanted $30 for a 10 oz bottle. This here is a 16 oz bottle for $18.99https://sbnf.net/products/oxygen-wellness-food-grade-hydrogen-peroxide-35***NOTE: I MAKE NOTHING FROM THE SALE OF THIS 35% H2O2 PRODUCT I just don't like greedy companies overcharging people who are desperately in need of a health intervention. I find it detestable. You can get a better deal elsewhere. Dr Ardis: https://theDrArdisShow.comCall-in: 619.354.8879 or On Telegram: https://t.me/BaalBustersStudiosPatreon: https://patreon.com/DisguisetheLimitsSubscribe Option 2: https://www.tipeeestream.com/baalbusters/SuperChat Alternative: https://www.buymeacoffee.com/baalbustersHot Sauce lovers, Go Here! I make it: https://SemperFryLLC.comBa'al Busters Subscribers get 11% off with Code: HSFAMILY plus a Free 2 oz Bonus sauce!Help the Show Remain: https://GiveSendGo.com/BaalBustersor https://paypal.me/BaalBustersThank You for the Much Needed Support!SHIRTS & MERCH https://my-store-c960b1.creator-spring.com/Get Healthy and Independent: https://riseupintohealth.com/?=ndhealthSubscribe to my Rumble Channel: https://rumble.com/c/c-1121444Subscribe on Brighteon: https://Brighteon.com/channels/BaalBustersFull Channel: https://BaalBuster.JoshWhoTV.comTelegram: https://t.me/BaalBustersStudiosSpotify: https://open.spotify.com/show/3N7fqqG6MX84vKbANtxrWSTik Tok: https://www.tiktok.com/@baalbusters (shadow banned)Twitter: https://twitter.com/DisguiseLimitsFREE Roku TV channel: https://channelstore.roku.com/details/a44cff88b32c2fcc7e090320c66c4d09/baal-busters-broadcastFor COPPERINE which my whole family uses, go here: https://BioChemScience.com and use BB2022 for Free ShippingThe Host, Daniel Kristos, is a US Coast Guard veteran, author, a father, small business owner, researcher, avid reader, and independent historian.

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Temporal Coordination of the Transcription Factor Response to H2O2 stress

Play Episode Listen Later Mar 7, 2023

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2023.03.07.531593v1?rss=1 Authors: Jose, E., March-Steinman, W., Wilson, B. A., Shanks, L., Paek, A. L. Abstract: The p53 and FOXO transcription factors (TFs) share many similarities despite their distinct evolutionary origins. Both TFs are activated by a variety of cellular stresses and upregulate genes in similar pathways including cell-cycle arrest and apoptosis. Oxidative stress from excess H2O2 activates both FOXO1 and p53, yet whether they are activated at the same time is unclear. Here we found that cells respond to high H2O2 levels in two temporal phases. In the first phase FOXO1 rapidly shuttles to the nucleus while p53 levels remain low. In the second phase FOXO1 exits the nucleus and p53 levels rise. We found that other oxidative stress induced TFs are activated in the first phase with FOXO1 (NF-{kappa}B, NFAT1), or the second phase with p53 (NRF2, JUN) but not both following H2O2 stress. The two TF phases result in large differences in gene expression patterns. Finally, we provide evidence that 2-Cys peroxiredoxins control the timing of the TF phases in response to H2O2. Copy rights belong to original authors. Visit the link for more info Podcast created by Paper Player, LLC

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Cellular protection from H2O2 toxicity by Fv-Hsp70 and mutants of Fv-Hsp70. Protection via catalase and gamma-glutamyl cysteine synthase.

Play Episode Listen Later Feb 22, 2023

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2023.02.22.529417v1?rss=1 Authors: Hino, C., Chan, G., Jordaan, G., Chang, S. S., Saunders, J., Bashir, M. T., Hansen, J. E., Gera, J., Weisbart, R. H., Nishimura, R. N. Abstract: Heat shock proteins (HSPs), especially Hsp70 (HSPA1), have been associated with cellular protection from various cellular stresses including heat, hypoxia-ischemia, neurodegeneration, toxins, and trauma. Endogenous HSPs are often synthesized in direct response to these stresses but in many situations are inadequate in protecting cells. The present study addresses the transduction of Hsp70 into cells providing protection from acute oxidative stress by H2O2. The recombinant Fv-Hsp70 protein and two mutant Fv-Hsp70 proteins minus the ATPase domain, and minus the ATPase and terminal lid domains were tested at 0.5 and 1.0 uM concentrations after two different concentrations of H2O2 treatment. All three recombinant proteins protected SH-SY5Y cells from acute H2O2 toxicity. This data indicated that the protein binding domain was responsible for cellular protection. In addition, experiments pretreating cells with inhibitors of antioxidant proteins catalase and gamma-glutamylcysteine synthase (GGCS) before H2O2 resulted in cell death despite treatment with Fv-Hsp70, implying that both enzymes were protected from acute oxidative stress after treatment with Fv-Hsp70. This study demonstrates that Fv-Hsp70 is protective in our experiments primarily by the protein-binding domain. The Hsp70 terminal lid domain was also not necessary for protection. Cellular protection was protective via the antioxidant proteins catalase and GGCS. Copy rights belong to original authors. Visit the link for more info Podcast created by Paper Player, LLC

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Visualizing H2O2 and NO in endothelial cells: strategies and pitfalls

Play Episode Listen Later Feb 16, 2023

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2023.02.15.528776v1?rss=1 Authors: Altun, H. Y., Secilmis, M., Akgul Caglar, T., Vatandaslar, E., Ozturk, G., Vilain, S. P., Eroglu, E. Abstract: The relationship between hydrogen peroxide (H2O2) and nitric oxide (NO) in the vasculature is multifaceted and remains controversial because the dynamic detection of these reactive molecules is challenging. Genetically encoded biosensors (GEBs) allow visualizing real-time dynamics in living cells and permit multiparametric detection of several analytes. Although robust, GEBs' utility depends on several parameters that need fine-tuning for proper imaging and correct data analysis: i.e., camera binning, temperature, and the resolution power of the imaging instruments are some critical parameters that require optimization. We have generated a new double-stable transgenic endothelial cell line stably expressing the biosensors HyPer7 and O-geNOp and systematically tested different imaging modes and their impact on the performance of each biosensor. Ambient temperature and the type of imaging mode did not influence the results, while camera resolution settings significantly affected readouts of HyPer probes but not O-geNOp. Changing a single parameter in a co-imaging mode significantly altered the biosensor's dynamic measurements, potentially causing misinterpretation. This study provides a general guide and the pitfalls of employing GEBs in a multispectral imaging mode. Copy rights belong to original authors. Visit the link for more info Podcast created by Paper Player, LLC

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Senescent response in inner annulus fibrosus cells in response to TNFα, H2O2, and TNFα-induced nucleus pulposus senescent secretome

Play Episode Listen Later Dec 22, 2022

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2022.12.21.521533v1?rss=1 Authors: Montgomery-Song, A., Ashraf, S., Santerre, P., Kandel, R. Abstract: Senescence, particularly in the nucleus pulposus (NP) cells, has been implicated in the pathogenesis of disc degeneration, however, the mechanism(s) of annulus fibrosus (AF) cell senescence is still not well understood. Both TNF and H2O2, have been implicated as contributors to the senescence pathways, and their levels are increased in degenerated discs when compared to healthy discs. This the objective of this study is to identify factor(s) that induces inner AF (iAF) cell senescence Under TNF exposure, at a concentration that can induce senescence in NP cells, bovine iAF cells did not undergo senescence, indicated by their ability to continue to proliferate as demonstrated by Ki67 staining and growth curves and lack of expression of the senescent markers, p16 and p21. Unlike iAF cells, NP cells treated with TNF accumulated more intracellular ROS and secreted more H2O2. Following TNF treatment, only iAF cells had increased expression of the superoxide scavengers SOD1 and SOD2 whereas NP cells had increased NOX4 gene expression, an enzyme that can generate H2O2. Treating iAF cells with low dose H2O2 (50 M) induced senescence, however unlike TNF, H2O2 did not induce degenerative-like changes as there was no difference in COL2, ACAN, MMP13, or IL6 gene expression or number of COL2 and ACAN immunopositive cells compared to untreated controls. The latter result suggests that iAF cells have distinct degenerative and senescent phenotypes. To evaluate paracrine signalling, iAF and TNF-treated NP cells were co-cultured. In contact co-culture the NP cells did induce iAF senescence. Thus, senescent NP cells may secrete soluble factors that induce degenerative and senescent changes within the iAF. This may contribute to a positive feedback loop of disc degeneration, and these processes could include H2O2 and cytokines (TNF). Further studies will investigate if human disc cells respond similarly. Copy rights belong to original authors. Visit the link for more info Podcast created by Paper Player, LLC

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Oxidative stress-induced MMP- and γ-secretase-dependent VE-cadherin processing is modulated by the proteasome and BMP9/10

Play Episode Listen Later Nov 24, 2022

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2022.11.23.517709v1?rss=1 Authors: Ivaldo, C., Passalacqua, M., Furfaro, A. L., d'Abramo, C., Ruiz, S., Chatterjee, P. K., Metz, C. N., Nitti, M., Marambaud, P. Abstract: Classical cadherins, including vascular endothelial (VE)-cadherin, are targeted by matrix metalloproteinases (MMPs) and {gamma}-secretase during adherens junction (AJ) disassembly, a mechanism that might have relevance for endothelial cell (EC) integrity and vascular homeostasis. Here, we show that oxidative stress triggered by H2O2 exposure induced efficient VE-cadherin proteolysis by MMPs and {gamma}-secretase in human umbilical endothelial cells (HUVECs). The cytoplasmic domain of VE-cadherin produced by {gamma}-secretase, VE-Cad/CTF2 - a fragment that has eluded identification so far - could readily be detected after H2O2 treatment. VE-Cad/CTF2, released into the cytosol, was tightly regulated by proteasomal degradation and was sequentially produced from an ADAM10/17-generated C-terminal fragment, VE-Cad/CTF1. Interestingly, BMP9 and BMP10, two circulating ligands critically involved in vascular maintenance, significantly reduced VE-Cad/CTF2 levels during H2O2 challenge, as well as mitigated H2O2- mediated actin cytoskeleton disassembly during VE-cadherin processing. Notably, BMP9/10 pretreatments efficiently reduced apoptosis induced by H2O2, favoring endothelial cell recovery. Thus, oxidative stress is a trigger of MMP- and {gamma}-secretase-mediated endoproteolysis of VE-cadherin and AJ disassembly from the cytoskeleton in ECs, a mechanism that is negatively controlled by the EC quiescence factors, BMP9 and BMP10. Copy rights belong to original authors. Visit the link for more info Podcast created by Paper Player, LLC

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Insights into H2O2-induced signaling in Jurkat cells from analysis of gene expression

Hellness to Wellness with Dr Jason West

Play Episode Listen Later Oct 21, 2022

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2022.10.20.513094v1?rss=1 Authors: Taylor, M. F., Black, M. A., Hampton, M. B., Ledgerwood, E. C. Abstract: Hydrogen peroxide (H2O2) is a ubiquitous oxidant produced in a regulated manner by various enzymes in mammalian cells. H2O2 reversibly oxidises thiol groups of cysteine residues to mediate intracellular signalling. Whilst examples of H2O2 dependent signalling have been reported, the exact molecular mechanism(s) of signalling and the pathways affected are not well understood. Here, the transcriptomic response of Jurkat T cells to H2O2 was investigated to determine global effects on gene expression. With a low H2O2 concentration (10 M) that did not induced an oxidative stress response or cell death, extensive changes in gene expression occurred after 4 hours (6803 differentially expressed genes). Of the genes with greater then 2-fold change in expression, 85% were upregulated suggesting that in a physiological setting H2O2 predominantly activates gene expression. Pathway analysis identified gene expression signatures associated with FOXO and NTRK signalling. These signatures were associated with an overlapping set of transcriptional regulators. Overall, our results provide a snapshot of gene expression changes in response to H2O2, which, along with further studies, will lead to new insights into the specific pathways that are activated in response to endogenous production of H2O2, and the molecular mechanisms of H2O2 signalling. Copy rights belong to original authors. Visit the link for more info Podcast created by Paper Player, LLC

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IV Hyrdrogen Peroxide Therapy

Play Episode Listen Later Oct 6, 2022 8:39

Intravenous Hydrogen Peroxide Therapy is an alternative health therapy that can be used to treat common infections such as cold, flu, sinus infections, or chronic infections like EBV and Lyme disease. Treatments are used to stimulate natural immunity. IV peroxide therapy contains a dilute dose of pharmaceutical-grade H2O2 along with vitamins and minerals. The infusion is introduced through a needle in the arm or hand and infused into the body for up to over 90 minutes. If you have questions about getting treatment for chronic infections here are the resources you should know about. https://www.westcliniconline.com/askmedicalquestion https://www.drjasonwest.live https://www.westcliniconline.com/new-patient-paperwork

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810 Medizin zum Selbermachen: Dr. Hartmut Fischer 3/3

Bio 360 - Zurück ins Leben | Energie und Gesundheit

Play Episode Listen Later Sep 23, 2022 48:39

Dr. Hartmut Fischer, bekannt durch das DMSO Handbuch, möchte die Medizin zurück zu den Menschen bringen. Wir sprechen über sein neues Buch „Medizin zum Selbermachen“ und stellen einige der Dinge vor, die jedermann kinderleicht zu Hause anwenden kann. Dies kann oftmals den Gang zum Mediziner sparen und führt zu Selbstwirksamkeit. Hol dir jetzt den ultimativen Schutz für deinen Darm und dein Immunsystem mit Gut Care von Braineffect. Mit dem Gutscheincode “bio360” bekommst du einen satten Rabatt! >>>Jetzt anschauen

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809 Medizin zum Selbermachen: Dr. Hartmut Fischer 2/3

Bio 360 - Zurück ins Leben | Energie und Gesundheit

Play Episode Listen Later Sep 21, 2022 36:24

Dr. Hartmut Fischer, bekannt durch das DMSO Handbuch, möchte die Medizin zurück zu den Menschen bringen. Wir sprechen über sein neues Buch „Medizin zum Selbermachen“ und stellen einige der Dinge vor, die jedermann kinderleicht zu Hause anwenden kann. Dies kann oftmals den Gang zum Mediziner sparen und führt zu Selbstwirksamkeit. Hol dir jetzt den ultimativen Schutz für deinen Darm und dein Immunsystem mit Gut Care von Braineffect. Mit dem Gutscheincode “bio360” bekommst du einen satten Rabatt! >>>Jetzt anschauen

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808 Medizin zum Selbermachen: Dr. Hartmut Fischer 1/3

Bio 360 - Zurück ins Leben | Energie und Gesundheit

Play Episode Listen Later Sep 19, 2022 31:50

Dr. Hartmut Fischer, bekannt durch das DMSO Handbuch, möchte die Medizin zurück zu den Menschen bringen. Wir sprechen über sein neues Buch „Medizin zum Selbermachen“ und stellen einige der Dinge vor, die jedermann kinderleicht zu Hause anwenden kann. Dies kann oftmals den Gang zum Mediziner sparen und führt zu Selbstwirksamkeit. Hol dir jetzt den ultimativen Schutz für deinen Darm und dein Immunsystem mit Gut Care von Braineffect. Mit dem Gutscheincode “bio360” bekommst du einen satten Rabatt! >>>Jetzt anschauen

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Episode 580 - Wow! My Own Label! - Historical Text Messaging - Alloys - Ladies Home Journal - Corporate America

Instant Trivia

Play Episode Listen Later Sep 13, 2022 7:56

Welcome to the Instant Trivia podcast episode 580, where we ask the best trivia on the Internet. Welcome to the Instant Trivia podcast episode 580, where we ask the best trivia on the Internet. Round 1. Category: Wow! My Own Label! 1: In the 1970s this "Rocket Man" founded Rocket Records. Elton John. 2: The 3 double CDs of the Beatle "Anthology" series are on this label founded by the Beatles. Apple. 3: He do be the founder of Reprise, yes he do be do be do. Frank Sinatra. 4: Alanis Morissette's "Supposed Former Infatuation Junkie" is on Maverick, this Material Girl's label. Madonna. 5: He's the best seller of all the artists in his own Paisley Park stable. Prince. Round 2. Category: Historical Text Messaging 1: 323 B.C.:chrgd w/impiety. nt ltng thm sin 2x vs. phlsphy. off 2 chalcis. lyceum ltr. Aristotle. 2: 1989:stk @ embassy.u.s. blsting vh @ me.:(ttyl. (Manuel) Noriega. 3: 1670s:dsgnd st. bride's chrch.englnd 4evr!. Christopher Wren. 4: 1981:am canada's pm. dont no zonker r b.d. u kp txtng me abt. pls stp. Pierre Trudeau. 5: 1890:wilhelm ii mkng me quit chnclr gig. off 2 est8s @ friedrichsruh, wch is impsbl 2 abbrv and b undrstd. (Otto von) Bismarck. Round 3. Category: Alloys 1: An alloy called babbitt metal is used to line bearings and bushings to reduce this. friction. 2: Simple carbon steels are just a little carbon and manganese and a lot of this. iron. 3: For this alloy, mix your zinc and copper in a crucible and work with your ingots after they cool. brass. 4: Adding platinum to gold turns it from yellow gold to this. white gold. 5: Osmiridium is, you guessed it, an alloy of this and iridium. osmium. Round 4. Category: Ladies Home Journal 1: It's the detachable part of an advertisement that entitles you to a discount at the supermarket. Coupon. 2: Examples of these include Dr. Atkins, the Macrobiotic and the Scarsdale. Diets. 3: This mild antiseptic with the formula H2O2 can also be used to bleach hair and fabrics. Hydrogen peroxide. 4: From the Latin for "entering", they're the listed things you need to prepare food from a recipe. Ingredients. 5: A housewife is also known as a homemaker or one of these "engineers". Domestic engineer. Round 5. Category: Corporate America 1: Selling "Little Dot" perfume door-to-door in 1886, Mrs. Albee is considered the 1st of these saleswomen. Avon lady. 2: This bookstore chain founded by Larry Hoyt in 1933 was named for an inspirational pond. Waldenbooks. 3: The "Al" in Alcoa stands for this. aluminum. 4: Automaker whose ad campaign centers around "The heartbeat of America". Chevrolet. 5: This company holds the patent on marking the sweetener Aspartame until 1992. NutraSweet. Thanks for listening! Come back tomorrow for more exciting trivia! Special thanks to https://blog.feedspot.com/trivia_podcasts/

Mussels. H2O2. Black Seed Oil

Simone Nicole

Play Episode Listen Later Sep 1, 2022 118:38

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Free radicals and antioxidants: Biochemistry:U.Satyanarayana

GarimaKushwaha The Medico

Play Episode Listen Later Mar 12, 2022 25:47

l-h" supply of oxygen is absolutely essential I for the existence of higher organisms. As the saying goes too much of even the best is bad. Very high concentrations of 02 are found to be toxic, and can damage tissues. The present day concept of oxygen toxicity is due to the involvement of oxygen free radicals or reactive oxygen species (ROS). In fact, the generation of reactive metabolites of 02 is an integral part of our daily life. A free radical is defined as a molecule or a mof ecular species that contains one or more unpaired electrons, and is capable of independent existence. Types of free radicals Oxygen is required in many metabolic reactions, particularly for the release of energy. During these processes, molecular O2 is completely reduced, and converted to water. However, if the reduction of 02 is incomplete, a series of reactive radicals are formed, as shown in the next column. r,r,.. (Molecular orygen) re- Y O, (Superoxide) h€,2H+ J H rO, (Hydrogen peroxide) l. e-,H' Hzo+1 + OH- (Hydroxyl radicat) h e-'H* + ,-r,r'(Water) Besides the above (O2, H2O2, OH-), the other free radicals and reactive oxygen species of biological importance include singlet oxygen (1O2), hydroperoxy radical (HOO-), lipid peroxide radical (ROO-), nitric oxide (NO-) and peroxyn itrite (ONOO-). The common characteristic features of free radicals are listed . Highly reactive o Very short half-lifeCan generate new radicals by chain reaction Cause damage to biomolecules, cells and tissues Free radicals and reactive oxygen species (ROS)-not synonymous : By definition, a free radical contains one or more unpaired electrons. e.g. Or, OH-, ROO-. There are certain non- radical derivatives of 02 which do not contain unpaired electrons e.g. H2O2, 102. The term reactive oxygen species is used in a broad sense to collectively represent free radicals, and non- free radicals (which are extremely reactive) of the biological systems. However, most authors do not make a clear distinction between free radicals and ROS, and use them inter- changebly. SOURCES AND GEI{ERATION OF FREE RADICALS The major sources responsible for the generation of free radicals may be considered under two categories l. Due to normal biological processes (or cellular metabolism). ll. Due to environmental effects. It is estimated that about 1-4o/o of the 02 taken up by the body is converted to free radicals. A summary of the sources for generation of free radicals is given in the Tahle 34.1 , and a couple of the processes are briefly described. Lipid peroxidation Free radical-induced peroxidation of membrane lipids occurs in three stages-initiation, propagation and termination Initiation phase : This step involves the removal of hydrogen atom (H) from polyunsaturated fatty acids (LH), caused by hydroxyl radical LH + OH- -----+ L- + H2O Propagation phase : Under aerobic conditions, the fatty acid radical (L-) takes up oxygen to form peroxy radical (LOO-). The I Cellular metabolism . Leakage of electrons lrom the respiratory chain (ETC). . Productionf HrO, or O, by oxidase enzymes (e.9. xanthine oxidase, NADPH oxidase). . Due to chain reactions ofmembrane lipid peroxidation. . Peroxisomal generation of O, and HrOr. . During the synthesis ofprostaglandins. . Productionf nitric oxide from arginine. . During the course of phagocytosis (as a pad of bactedcidal action). . In the oxidation ofheme to bile pigments. . As a result of auteoxidation e.g. metal ions [Fd*, Cu2*]; ascorbic acid, glutathione, flavin coenzymes. II Environmental effects . As a result of drug melabolism e.g. paracetamol, halothane, cy{ochrome P* rehted reactions. . Due to damages caused by ionizing radiations (e.9. X-rays) on tissues. . Photolysis of O, by light. . Photoexcitation of organic molecules . Cigarette smoke contains free radicals, and trace metals that generate OH-.Alcohol, promoting lipid peroxidation.

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S3 ep 6 [ # 69 ]: Bucks do YouTube, Clothing perils, More the hard way, What is H2O2?, Lagrange Point L2

Two Old Bucks

Play Episode Listen Later Feb 10, 2022 29:32

Bucks finally launch their YouTube Channel. You can google "Two old bucks youtube" or use this link. Every journey starts with the first step so count on us to travel through time and space looking for we don't know what.Washington Football Team is now the Commanders. Del has some ideas on how this will spin out. Less than a month after we introduce WORDLE to our vast audience, the NewYork Times buys WORDLE for seven figures. Our 10% fee is looking sweet.Del, planning for future expansion, breaks down and buys a new belt while Dave is banned from wearing tank tops outside the house. So many chances to do it the hard way. Even drinking hydrogen peroxide [do not try this at home]. The Webb telescope reaches the L2 Lagrange Point [sigh of relief] .

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163 Speedy, Successful Seed Germinating Tips

Garden Basics with Farmer Fred

Play Episode Listen Later Jan 28, 2022 39:55 Transcription Available

'Tis the season for expectant backyard gardeners. You've begun to sow seeds of warm season annuals and vegetables indoors, especially pepper and tomato seeds. But when will those little seeds begin to show their heads above the soil? As always, it depends. On today's Garden Basics with Farmer Fred podcast, our favorite retired college horticultural professor, Debbie Flower, has tips for speeding up seed germination rates, especially for those pokey pepper seeds, which normally could take up to three weeks to sprout! But, it takes a combination of tactics to be a successful speedy plant parent. It involves bleach, the right containers, heat, the correct application of water, the right soil mix, proper air movement and H2O2. Welcome back to high school chemistry class, kids. But, you can do it! Today, it's speedy, successful seed germination tips, especially for the pokey peppers.We're podcasting from Barking Dog Studios here in the beautiful Abutilon Jungle in Suburban Purgatory, it's the Garden Basics with Farmer Fred podcast, brought to you today by Smart Pots and Dave Wilson Nursery. And we will do it all in a skosh over 30 minutes (more like 40 minutes). Let's get started!November through January, the Garden Basics with Farmer Fred podcast slows its production schedule. Look for new episodes each Friday. In February, we will return to twice a week podcasts, on Tuesdays and Fridays.Pictured:Pepper Seed PacketsLinks:Subscribe to the free, Garden Basics with Farmer Fred Newsletter Smart PotsDave Wilson NurseryGrow lightsDramm Water Breaker nozzlePump Pressure Water SprayerSeed Starting trays, kitsIndoor GreenhousesOutdoor GreenhousesSeed Starting MixBaker Creek Seed CatalogMore episodes and info available at Garden Basics with Farmer FredLive links, product information, transcripts, and chapters available at the Buzzsprout home site for Garden Basics with Farmer Fred. Got a garden question? • Leave an audio question without making a phone call via Speakpipe, at https://www.speakpipe.com/gardenbasics• Call or text us the question: 916-292-8964. • E-mail: fred@farmerfred.com All About Farmer Fred:The Garden Basics with Farmer Fred Newsletter Farmer Fred website: http://farmerfred.comDaily Garden tips and snark on TwitterThe Farmer Fred Rant! BlogFacebook: "Get Growing with Farmer Fred"Instagram: farmerfredhoffmanFarmer Fred Garden Videos on YouTubeAs an Amazon Associate, I earn from qualifying purchases from possible links mentioned here.And thank you for listening.

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DNA spikey, enzyme no likey – monitoring H2O2 live, seed inspiration, cheatgrass

Plants and Pipettes

Play Episode Listen Later Oct 1, 2021 60:47

Some people say, Joram is too close to still being sick in this episode. Those people are wrong. Joram is just a pretentious stuck up golfer who will never speak again with air freely travelling through his nose. So there's that. Also, we have plant science for (for real!) and other… stuff. Like a stuffy nose. Bless you.

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The Gary Null Show - 05.06.21

The Gary Null Show

Play Episode Listen Later May 6, 2021 59:50

Treatment with Rhodiola mimics exercise to resist high-fat diet-induced muscle dysfunction Central South University (China), April 30, 2021 According to news reporting out of Changsha, People’s Republic of China, research stated, “Muscle dysfunction is a complication of high-fat diet (HFD)-induced obesity that could be prevented by exercise, but patients did not get enough therapeutic efficacy from exercise due to multiple reasons.” The news reporters obtained a quote from the research from Xiangya Hospital of Central South University: “To explore alternative or supplementary approaches to prevent or treat muscle dysfunction in individuals with obesity, we investigated the effects of Rhodiola on muscle dysfunction as exercise pills. SIRT1 might suppress atrogenes expression and improve mitochondrial quality control, which could be a therapeutic target stimulated by exercise and Rhodiola, but further mechanisms remain unclear. We verified the lipid metabolism disorders and skeletal muscle dysfunction in HFD feeding mice. Moreover, exercise and Rhodiola were used to intervene mice with a HFD. Our results showed that exercise and Rhodiola prevented muscle atrophy and dysfunction in obese mice and activating the SIRT1 pathway, while atrogenes were suppressed and mitochondrial quality control was improved. EX-527, SIRT1 inhibitor, was used to validate the essential role of SIRT1 in salidroside benefit.” According to the news editors, the research concluded: “Results of cell culture experiment showed that salidroside alleviated high palmitate-induced atrophy and mitochondrial quality control impairments, but these improvements of salidroside were inhibited by EX-527 in C2C12 myotubes. Overall, Rhodiola mimics exercise that activates SIRT1 signaling leading to improvement of HFD-induced muscle dysfunction.” Prenatal exposure to pesticides increases the risk of obesity in adolescence First study to analyse the long-term effects of persistent organic pollutants on cardiometabolic risk in adolescents Barcelona Institute for Global Health (Spain), May 3, 2021 Exposure before birth to persistent organic pollutants (POPs)-- organochlorine pesticides, industrial chemicals, etc.--may increase the risk in adolescence of metabolic disorders, such as obesity and high blood pressure. This was the main conclusion of a study by the Barcelona Institute for Global Health (ISGlobal), a research centre supported by the "la Caixa" Foundation. The study was based on data from nearly 400 children living in Menorca, who were followed from before birth until they reached 18 years of age. POPs are toxic, degradation-resistant chemicals that persist in the environment. Examples of such compounds are pesticides and organochlorine insecticides (DDT, etc.). POPs have adverse effects on both human health and the environment and their use is regulated globally. Prenatal exposure to these substances has been associated with cardiometabolic risk factors in childhood, but there were previously no studies assessing whether such associations continue into adolescence, a developmental stage characterised by significant changes in the endocrine system and rapid increases in body mass. The aim of this investigation, carried out within the framework of the INMA Project-Environment and Childhood, was to study the associations between prenatal exposure to POPs and body mass index (BMI) as well as other markers of cardiovascular risk in adolescence. Data from 379 children in Menorca was analysed. POP levels were measured in umbilical cord blood samples and the children were then seen periodically between the ages of 4 and 18 years. At these visits, BMI, body fat percentage and blood pressure were recorded as they grew. When the child reached 14 years of age, the scientists measured blood biomarkers of cardiometabolic risk (cholesterol, triglycerides, glucose, etc.). The results of this study, published in the journal Environment International, suggest an association between prenatal POP exposure and a higher BMI in adolescence, particularly in the case of the fungicide hexachlorobenzene (HCB) and the insecticide compound dichloro-diphenyl-trichloroethane (DDT). Exposure to these two organochlorides--HCB and DDT¬--was also associated with higher blood pressure in childhood and adolescence and increased cardiometabolic risk at 14 years of age. ISGlobal researcher Núria Güil-Oumrait, the first author of the study, explains that "this is the first longitudinal study to analyse the relationship between persistent organic pollutants and cardiometabolic risk throughout childhood and adolescence. Our findings show that the association between these substances and infant BMI does persist into adolescence and that prenatal exposures are associated with the main risk factors for metabolic syndrome in adults, a condition that today affects one in four people worldwide. With respect to the mechanisms that might explain this association, Güil-Oumrait points out that "it is thought that POPs may interact with hormone receptors or with the generation of free radicals, and the chief problem is that these pollutants accumulate in the fatty tissues of living organisms, where they can persist for years, even decades". Martine Vrijheid, study coordinator and head of the Childhood and Environment Programme at ISGlobal, highlights the fact that "some of these substances could be considered endocrine disruptors, that is, chemicals that interfere with hormonal regulation". In her view "more studies are needed in this field, especially focussing on childhood and adolescence, which are critical developmental stages characterised by particular vulnerability". One cup of leafy green vegetables a day lowers risk of heart disease Research has found that by eating just one cup of nitrate-rich vegetables each day people can significantly reduce their risk of heart disease. Edith Cowan University (Australia), May 4, 2021 New Edith Cowan University (ECU) research has found that by eating just one cup of nitrate-rich vegetables each day people can significantly reduce their risk of heart disease. The study investigated whether people who regularly ate higher quantities of nitrate-rich vegetables, such as leafy greens and beetroot, had lower blood pressure, and it also examined whether these same people were less likely to be diagnosed with heart disease many years later. Cardiovascular diseases are the number one cause of death globally, taking around 17.9 million lives each year. Researchers examined data from over 50,000 people residing in Denmark taking part in the Danish Diet, Cancer, and Health Study over a 23-year period. They found that people who consumed the most nitrate-rich vegetables had about a 2.5 mmHg lower systolic blood pressure and between 12 to 26 percent lower risk of heart disease. Lead researcher Dr Catherine Bondonno from ECU's Institute for Nutrition Research said identifying diets to prevent heart disease was a priority. "Our results have shown that by simply eating one cup of raw (or half a cup of cooked) nitrate-rich vegetables each day, people may be able to significantly reduce their risk of cardiovascular disease," Dr Bondonno said. "The greatest reduction in risk was for peripheral artery disease (26 percent), a type of heart disease characterised by the narrowing of blood vessels of the legs, however we also found people had a lower risk of heart attacks, strokes and heart failure." Forget the supplements The study found that the optimum amount of nitrate-rich vegetables was one cup a day and eating more than that didn't seem to give any additional benefits. "People don't need to be taking supplements to boost their nitrate levels because the study showed that one cup of leafy green vegetables each day is enough to reap the benefits for heart disease," Dr Bondonno said. "We did not see further benefits in people who ate higher levels of nitrate rich vegetables." Smoothies are ok Dr Bondonno said hacks such as including a cup of spinach in a banana or berry smoothie might be an easy way to top up our daily leafy greens. "Blending leafy greens is fine, but don't juice them. Juicing vegetables removes the pulp and fibre," Dr Bondonno said. The paper "Vegetable nitrate intake, blood pressure and incident cardiovascular disease: Danish Diet, Cancer, and Health Study" is published in the European Journal of Epidemiology. It is a collaboration between Edith Cowan University, the Danish Cancer Society and The University of Western Australia. The research adds to growing evidence linking vegetables generally and leafy greens specifically with improved cardiovascular health and muscle strength. This evidence includes two recent ECU studies exploring cruciferous vegetables and blood vessel health and green leafy vegetables and muscle strength. Mindfulness programs can boost children's mental health University of Derby (UK), May 4, 2021 Mindfulness programs can improve the mental health of school-age children and help them to feel more optimistic, according to new research from the University of Derby and Derbyshire Educational Psychology Service. More than 1,000 pupils aged between 9-12 years old across 25 schools in Derbyshire, received one 45-minute mindfulness session per week for nine weeks during the year-long project, which involved a collaboration between Dr. William Van Gordon, Associate Professor in Contemplative Psychology at the University, and Derbyshire Educational Psychology Service. Mindfulness is an ancient meditation technique that involves focussing awareness on the present moment, as a means of fostering calm, wellbeing and insight. The weekly sessions involved activities such as practicing mindful breathing and paying attention to bodily sensations, as well as exercises intended to help cultivate attention skills and a greater awareness of emotions. The impact of the sessions, which were delivered by teachers in a traditional classroom environment, was evaluated by comparing psychological assessments that the children completed before the classes began, with assessments undertaken after the program had concluded. Part of the evaluation measured children's emotional resiliency using The Resiliency Scale for Children, while wellbeing was rated using the Stirling Children's Wellbeing Scale. Overall, the study found a significant improvement in positive emotional state, outlook and resiliency. There was also an increase in the different dimensions of resilience: optimism increased by 10%, tolerance was improved by 8% and self-efficacy, how a child feels they can cope with a situation based on the skills they have and the circumstances they face, improved by 11%. Professor Van Gordon said: "Findings from the study indicate that mindfulness delivered by school teachers can improve wellbeing and resiliency in children and young people. "This is consistent with wider evidence demonstrating the positive impact of mindfulness on school children's levels of emotional resiliency, emotional stability, wellbeing and stress. "These findings are also in line with the view that preventative interventions given at a young age can help to reduce the incidence of mental health problems in young people." Vitamin D levels higher in exercisers Johns Hopkins University, May 01 2021 The issue of the Journal of Clinical Endocrinology & Metabolism published the finding of researchers at Johns Hopkins University of a correlation between increased physical activity and higher levels of vitamin D. Higher levels of vitamin D and exercise was also associated with a lower risk of cardiovascular disease. The study included 10,342 men and women who were free of coronary heart disease and heart failure upon enrollment in the Atherosclerosis Risk in Communities study. Physical activity levels were assessed during follow-up visits that took place over a 19.3-year period. Stored serum samples obtained at the second visit were analyzed for 25-hydroxyvitamin D3. Subjects who achieved American Heart Association recommended physical activity levels had average levels of vitamin D that were higher than those who had intermediate and poor levels of activity. Following adjustment for lifestyle and other factors, those who met the recommended levels had a 31% lower risk of being deficient in vitamin D than those with poor activity levels. Subjects in the recommended activity group with levels of vitamin D of 30 ng/mL or more had a 24% lower risk of cardiovascular disease. The association between exercise and vitamin D was stronger in subjects of European ethnicity than among African Americans. The authors noted that European-Americans as well as those who engage in exercise are likelier to be supplement users. “We did find that vitamin D supplement use was higher among those with increased physical activity,” they observed. "In our study, both failure to meet the recommended physical activity levels and having vitamin D deficiency were very common" stated coauthor Erin Michos, MD, MS, of Johns Hopkins University School of Medicine. "The bottom line is we need to encourage people to move more in the name of heart health." One teaspoon daily of trehalose can help maintain glucose homeostasis: a double-blind, randomized controlled trial Hayashibara Co. Ltd (Japan), April 24, 2021 Background Trehalose is a natural disaccharide that is widely distributed. A previous study has shown that daily consumption of 10 g of trehalose improves glucose tolerance in individuals with signs of metabolic syndrome. In the present study, we determined whether a lower dose (3.3 g/day) of trehalose improves glucose tolerance in healthy Japanese volunteers. Methods This was a randomized, double-blind, placebo-controlled study of healthy Japanese participants (n = 50). Each consumed 3.3 g of trehalose (n = 25) or sucrose (n = 25) daily for 78 days. Their body compositions were assessed following 0, 4, 8, and 12 weeks; and serum biochemical parameters were assayed and oral 75-g glucose tolerance tests were performed at baseline and after 12 weeks. Results There were similar changes in body composition and serum biochemistry consistent with established seasonal variations in both groups, but there were no differences in any of these parameters between the two groups. However, whereas after 12 weeks of sucrose consumption, the plasma glucose concentration 2 h after a 75-g glucose load was significantly higher than the fasting concentration, after 12 weeks of trehalose consumption the fasting and 2-h plasma glucose concentrations were similar. Furthermore, an analysis of the participants with relatively high postprandial blood glucose showed that the plasma glucose concentration 2 h after a 75-g glucose load was significantly lower in the trehalose group than in the sucrose group. Conclusions Our findings suggest that trehalose helps lower postprandial blood glucose in healthy humans with higher postprandial glucose levels within the normal range, and may therefore contribute to the prevention of pathologies that are predisposed to by postprandial hyperglycemia,, even if the daily intake of trehalose is only 3.3 g, an amount that is easily incorporated into a meal. Coffee compound enhances autophagy to protect against cell injury Chengdu University of Traditional Chinese Medicine (China), April 30, 2021 According to news reporting originating from Sichuan, People’s Republic of China, research stated, “Autophagy serves an important role in amyloid-beta (A beta) metabolism and tau processing and clearance in Alzheimer’s disease. The progression of A beta plaque accumulation and hyperphosphorylation of tau proteins are enhanced by oxidative stress.” Our news editors obtained a quote from the research from the Chengdu University of Traditional Chinese Medicine, “A hydrogen peroxide (H2O2) injury cell model was established using SH-SY5Y cells. Cells were randomly divided into normal, H2O2 and chlorogenic acid (5-caffeoylquinic acid; CGA) groups. The influence of CGA on cell viability was evaluated using a Cell Counting Kit-8 assay and cell death was assessed using Hoechst 33342 nuclear staining. Autophagy induction and fusion of autophagic vacuoles assays were performed using monodansylcadaverine staining. Additionally, SH-SY5Y cells expressing Ad-mCherry-green fluorescent protein-LC3B were established to detect autophagic flow. LysoTracker Red staining was used to evaluate lysosome function and LysoSensor ™ Green staining assays were used to assess lysosomal acidification. The results demonstrated that CGA decreased the apoptosis rate, increased cell viability and improved cell morphology in H2O2-treated SH-SY5Y cells. Furthermore, CGA alleviated the accumulation of autophagic vacuoles, reduced the LC3BII/I ratio and decreased P62 levels, resulting in increased autophagic flux. Additionally, CGA upregulated lysosome acidity and increased the expression levels of cathepsin D. Importantly, these effects of CGA on H2O2-treated SH-SY5Y cells were mediated via the mTOR-transcription factor EB signaling pathway.” According to the news editors, the research concluded: “These results indicated that CGA protected cells against H2O2-induced oxidative damage via the upregulation of autophagosomes, which promoted autophagocytic degradation and increased autophagic flux.” This research has been peer-reviewed.

看看海有什麼塑？黑潮海洋文教基金會島航計畫（下）

IC之音｜島嶼共聲．傾聽台灣

Play Episode Listen Later Jan 5, 2021 24:19

你知道，塑膠微粒的問題有多嚴重嗎？2018年環保署進行首次針對自來水、海水、沙灘砂礫，與貝類生物體當中的微型塑膠進行調查，結果大部分樣品均檢出微型塑膠，自來水中檢出塑膠數量為每公升0至6根；海水微型塑膠數量每1,000公升有1,000至18,500個，沙灘砂礫每公斤26至2,400個，養殖及野生貝類每公克0.2到5.2個。不止官方調查，民間的研究結果也顯示出問題的嚴重性。黑潮海洋文教基金會2018年完成「島航計畫」，總共51個測點的表層海水，都發現有塑膠微粒。在2019、2020年的「島航普拉斯」計畫，針對先前調查污染最嚴重之台灣東北及西南海域共24個測點，進行每一季的採樣與分析，結果每個測點、每個季節皆發現塑膠微粒，其中，夏季的塑膠微粒數量最多，發泡塑膠微粒的含量明顯高於其他季節2-8倍。此計畫運用Manta網採集表層海水之塑膠微粒樣本，此為美國非營利組織5 Gyres Institute所設計的網具，外型類似鬼蝠魟（manta ray），左右兩片漂浮板，後面拖著長長的網袋，像是魟魚的尾巴。Manta網有不同的尺寸，黑潮海洋文教基金會使用的Manta網寬180公分，網袋長150公分，加上身體總長也有180公分，網袋網目為330微米，收集開口長60公分，寬13公分。研究船在抵達測點之後，會紀錄流速計起始數值、拖網起始時間、測點經緯度等採樣數值，及天氣、水溫、鹽度、水深等環境數值。同時把Manta網放下去海水，以平均船速約2節（約3.7公里/時）非常緩慢的速度，拖行15分鐘進行採樣。拖行結束，將Manta網拉起來後，研究人員會將樣本全數沖洗到樣本瓶裡面，隨即加入70%酒精溶液，標示採樣地點後保存。回到實驗室後，參照2015年美國國家海洋及大氣協會（NOAA）之海洋塑膠微粒實驗室分析標準方法，處理樣本。首先將所有樣本倒出，經過篩網過濾，挑出放置於燒杯。因為樣本中包含生物體、非生物體，接著研究人員會在燒杯中會加入適量的二價鐵（FeSO4）溶液，與等量的30%過氧化氫（H2O2），將生物體分解。生物體分解後，樣本經篩網過濾，以大量去離子水沖洗，去除生物體；接著放置於解剖顯微鏡下觀察，挑出塑膠微粒放置於培養皿上，培養皿會先放置間隔為0.5公分之方格紙以利進行計數，並依塑膠微粒材質分成五大類：硬塑膠（fragments，大型塑膠製品的碎片，如寶特瓶、包裝盒、玩具等）、軟塑膠（films，如塑膠袋、包裝紙）、發泡塑膠（foams，如保麗龍）、塑膠纖維（lines/fibers，如釣魚線、漁網或人造纖維產品）、圓形塑膠粒（pellets，為塑膠原料），分別計算各類型數量。黑潮海洋文教基金會研究員温珮珍指出，根據2019-2020年的調查結果，台灣東北及西南海域每個測點、每個季節皆發現塑膠微粒，其中東北海域塑膠微粒數量遠高於西南海域，甚至在夏、秋兩季甚至可達20倍之差距。若以材質區分，硬塑膠佔比最高，幾乎每個季節及測點皆有硬塑膠存在，可見生活塑膠仍佔大宗。若以季節來看，塑膠微粒數量最多的季節在夏季，其中又以主要來源為保麗龍的發泡塑膠微粒，含量明顯高於其他季節2-8倍。雖然西南海域在採樣季節及地點上無明顯差異，但在安平新港及曾文溪出海口可以發現，夏季時塑膠微粒數量偏高，且發泡塑膠佔九成以上。調查推測，夏季發泡塑膠可能為牡蠣養殖之廢棄物。研究人員使用科技部海洋學門資料庫網站進行漂流軌跡模擬，顯示這些廢棄物會先隨著海流北上，到台灣北方海域後，受東北角的渦流牽引，移動速度變慢並往東邊及東南向漂流，到基隆時可能在基隆與三貂角之間渦流當中打轉，最後可能被沖上岸堆積，也可能被推到三貂角外脫離渦流，隨著黑潮流向日本。加上台灣海域目視海漂垃圾調查結果、海洋大學模擬颱風季節漂流木在海上移動情形，推測夏季東北海域的塑膠微粒，非常可能與西南沿海養蚵產業有關。當然，確切實證則需進一步調查才能夠確認。有了上述調查結果，黑潮海洋文教基金會也提出相關倡議。在牡蠣養殖之廢棄物方面，雖然台南市政府宣示三年內汰換保麗龍浮具，補助漁民將保麗龍更換為發泡聚丙烯（EPP）材質等政策，同時也投入新式浮具開發，但EPP材質仍會碎裂，新浮具開發也在初期階段，希望各相關單位能加快腳步。另外，除了夏季外，其他季節的塑膠微粒還是以硬塑膠的比例最高，雖然環保署提出減塑的政策與目標，不過具體執行還是落在我們每個使用者身上。包括企業可以思考改變包裝方式，減少一次性用品的使用，民眾也盡量使用可以重複使用的器具，做好3R：減量、再利用、回收，並支持做出改變的企業。我們接著上禮拜的主題，前往花蓮拜訪黑潮海洋文教基金會，研究員温珮珍將分享島航計畫中，關於塑膠微粒的調查結果與發現，也希望您一同響應，從生活中做出改變，減少塑膠製品與一次性產品的使用。

noaa manta epp 3r h2o2 gyres institute

看看海有什麼塑？黑潮海洋文教基金會島航計畫（下）

IC之音｜島嶼共聲．傾聽台灣

Play Episode Listen Later Jan 5, 2021 24:19

你知道，塑膠微粒的問題有多嚴重嗎？2018年環保署進行首次針對自來水、海水、沙灘砂礫，與貝類生物體當中的微型塑膠進行調查，結果大部分樣品均檢出微型塑膠，自來水中檢出塑膠數量為每公升0至6根；海水微型塑膠數量每1,000公升有1,000至18,500個，沙灘砂礫每公斤26至2,400個，養殖及野生貝類每公克0.2到5.2個。不止官方調查，民間的研究結果也顯示出問題的嚴重性。黑潮海洋文教基金會2018年完成「島航計畫」，總共51個測點的表層海水，都發現有塑膠微粒。在2019、2020年的「島航普拉斯」計畫，針對先前調查污染最嚴重之台灣東北及西南海域共24個測點，進行每一季的採樣與分析，結果每個測點、每個季節皆發現塑膠微粒，其中，夏季的塑膠微粒數量最多，發泡塑膠微粒的含量明顯高於其他季節2-8倍。此計畫運用Manta網採集表層海水之塑膠微粒樣本，此為美國非營利組織5 Gyres Institute所設計的網具，外型類似鬼蝠魟（manta ray），左右兩片漂浮板，後面拖著長長的網袋，像是魟魚的尾巴。Manta網有不同的尺寸，黑潮海洋文教基金會使用的Manta網寬180公分，網袋長150公分，加上身體總長也有180公分，網袋網目為330微米，收集開口長60公分，寬13公分。研究船在抵達測點之後，會紀錄流速計起始數值、拖網起始時間、測點經緯度等採樣數值，及天氣、水溫、鹽度、水深等環境數值。同時把Manta網放下去海水，以平均船速約2節（約3.7公里/時）非常緩慢的速度，拖行15分鐘進行採樣。拖行結束，將Manta網拉起來後，研究人員會將樣本全數沖洗到樣本瓶裡面，隨即加入70%酒精溶液，標示採樣地點後保存。回到實驗室後，參照2015年美國國家海洋及大氣協會（NOAA）之海洋塑膠微粒實驗室分析標準方法，處理樣本。首先將所有樣本倒出，經過篩網過濾，挑出放置於燒杯。因為樣本中包含生物體、非生物體，接著研究人員會在燒杯中會加入適量的二價鐵（FeSO4）溶液，與等量的30%過氧化氫（H2O2），將生物體分解。生物體分解後，樣本經篩網過濾，以大量去離子水沖洗，去除生物體；接著放置於解剖顯微鏡下觀察，挑出塑膠微粒放置於培養皿上，培養皿會先放置間隔為0.5公分之方格紙以利進行計數，並依塑膠微粒材質分成五大類：硬塑膠（fragments，大型塑膠製品的碎片，如寶特瓶、包裝盒、玩具等）、軟塑膠（films，如塑膠袋、包裝紙）、發泡塑膠（foams，如保麗龍）、塑膠纖維（lines/fibers，如釣魚線、漁網或人造纖維產品）、圓形塑膠粒（pellets，為塑膠原料），分別計算各類型數量。黑潮海洋文教基金會研究員温珮珍指出，根據2019-2020年的調查結果，台灣東北及西南海域每個測點、每個季節皆發現塑膠微粒，其中東北海域塑膠微粒數量遠高於西南海域，甚至在夏、秋兩季甚至可達20倍之差距。若以材質區分，硬塑膠佔比最高，幾乎每個季節及測點皆有硬塑膠存在，可見生活塑膠仍佔大宗。若以季節來看，塑膠微粒數量最多的季節在夏季，其中又以主要來源為保麗龍的發泡塑膠微粒，含量明顯高於其他季節2-8倍。雖然西南海域在採樣季節及地點上無明顯差異，但在安平新港及曾文溪出海口可以發現，夏季時塑膠微粒數量偏高，且發泡塑膠佔九成以上。調查推測，夏季發泡塑膠可能為牡蠣養殖之廢棄物。研究人員使用科技部海洋學門資料庫網站進行漂流軌跡模擬，顯示這些廢棄物會先隨著海流北上，到台灣北方海域後，受東北角的渦流牽引，移動速度變慢並往東邊及東南向漂流，到基隆時可能在基隆與三貂角之間渦流當中打轉，最後可能被沖上岸堆積，也可能被推到三貂角外脫離渦流，隨著黑潮流向日本。加上台灣海域目視海漂垃圾調查結果、海洋大學模擬颱風季節漂流木在海上移動情形，推測夏季東北海域的塑膠微粒，非常可能與西南沿海養蚵產業有關。當然，確切實證則需進一步調查才能夠確認。有了上述調查結果，黑潮海洋文教基金會也提出相關倡議。在牡蠣養殖之廢棄物方面，雖然台南市政府宣示三年內汰換保麗龍浮具，補助漁民將保麗龍更換為發泡聚丙烯（EPP）材質等政策，同時也投入新式浮具開發，但EPP材質仍會碎裂，新浮具開發也在初期階段，希望各相關單位能加快腳步。另外，除了夏季外，其他季節的塑膠微粒還是以硬塑膠的比例最高，雖然環保署提出減塑的政策與目標，不過具體執行還是落在我們每個使用者身上。包括企業可以思考改變包裝方式，減少一次性用品的使用，民眾也盡量使用可以重複使用的器具，做好3R：減量、再利用、回收，並支持做出改變的企業。我們接著上禮拜的主題，前往花蓮拜訪黑潮海洋文教基金會，研究員温珮珍將分享島航計畫中，關於塑膠微粒的調查結果與發現，也希望您一同響應，從生活中做出改變，減少塑膠製品與一次性產品的使用。

noaa manta epp 3r h2o2 gyres institute

Episode 1 - Dogs, Jobs (Again), and Eric Andre

Hoofenanny: The Off Topic Series

Play Episode Listen Later Nov 22, 2020 36:18

Pete has a cute puppy whose picky, jobs are a struggle to get nowadays, and who else is excited for the Season Finale of Eric Andre Season 5? H2O2 is the greatest sequel to water! --- Support this podcast: https://podcasters.spotify.com/pod/show/hoofenanny-podcast/support

dogs jobs season finale eric andre h2o2

Age-Associated Insolubility of Parkin in Human Midbrain is Linked to Redox Balance and Sequestration of Reactive Dopamine Metabolites

PaperPlayer biorxiv biophysics

Play Episode Listen Later Nov 21, 2020

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2020.11.20.392175v1?rss=1 Authors: Tokarew, J. M., El Kodsi, D. N., Lengacher, N. A., Fehr, T. K., Nguyen, A. P., Shutinoski, B., O'Nuallain, B., Jin, M., Khan, J., Ng, A. C. H., Li, J., Jiang, Q., Zhang, M., Wang, L., Sengupta, R., Barber, K., Tran, A., Zandee, S., Dong, X., Scherzer, C. R., Prat, A., Tsai, E., Takanashi, M., Hattori, N., Chan, J. A., Zecca, L., West, A., Holmgren, A., Puente, L., Shaw, G. S., Toth, G., Woulfe, J., Taylor, P., Tomlinson, J. J., Schlossmacher, M. G. Abstract: The mechanisms by which parkin protects the adult human brain from Parkinson disease remain incompletely understood. We hypothesized that parkin cysteines participate in redox reactions, which are reflected in its posttranslational modifications. We found that in human control brain, including the S. nigra, parkin is largely insoluble after age 40 years, which is linked to its oxidation, e.g., at Cys95 and Cys253. In mice, oxidative stress increases posttranslational modifications at parkin cysteines and reduces its solubility. Oxidation of recombinant parkin also promotes insolubility and aggregate formation, but in parallel, lowers hydrogen peroxide (H2O2). This thiol-based redox activity is diminished by parkin point mutants, e.g., p.C431F and p.G328E. Intriguingly, in parkin-deficient human brain H2O2 concentrations are elevated. In prkn-null mice, H2O2 levels are dysregulated under oxidative stress conditions, such as acutely by MPTP-toxin exposure or chronically due to a second genetic hit. In dopamine toxicity studies, wild-type parkin, but not disease-linked mutants, protects human dopaminergic M17 cells, in part through lowering H2O2. Parkin also neutralizes reactive, electrophilic dopamine metabolites via adduct formation, which occurs foremost at primate-specific Cys95. Further, wild-type but not p.C95A-mutant parkin augments melanin formation. In sections of normal, adult human midbrain, parkin specifically co-localizes with neuromelanin pigment, frequently within LAMP-3/CD63+ lysosomes. We conclude that oxidative modifications of parkin cysteines are associated with protective outcomes, which include the reduction of H2O2, conjugation of reactive dopamine metabolites, sequestration of radicals within insoluble aggregates, and increased melanin formation. The loss of these redox effects may augment oxidative stress in dopamine producing neurons of mutant PRKN allele carriers, thereby contributing to neurodegeneration. Copy rights belong to original authors. Visit the link for more info

Insights into G-Quadruplex-Hemin Dynamics Using Atomistic Simulations: Implications for Reactivity and Folding

Play Episode Listen Later Nov 10, 2020

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2020.11.09.375691v1?rss=1 Authors: Stadlbauer, P., Islam, B., Otyepka, M., Chen, J., monchaud, d., Zhou, J., Mergny, J.-L., Sponer, J. Abstract: Guanine quadruplex nucleic acids (G4s) are involved in key biological processes such as replication or transcription. Beyond their biological relevance, G4s find applications as biotechnological tools since they readily bind hemin and enhance its peroxidase activity, creating a G4-DNAzyme. The biocatalytic properties of G4-DNAzymes have been thoroughly studied and used for biosensing purposes. Despite hundreds of applications and massive experimental efforts, the atomistic details of the reaction mechanism remain unclear. To help select between the different hypotheses currently under investigation, we use extended explicit-solvent molecular dynamics simulations to scrutinize the G4/hemin interaction. We found that besides the dominant conformation in which hemin is stacked atop the external G-quartets, hemin can also transiently bind to the loops and be brought to the external G-quartets through diverse delivery mechanisms. Importantly, the simulations do not support several mechanistic possibilities (i.e., the wobbling guanine and the iron-bound water molecule) but rather suggest tentative mechanisms in which the external G-quartet itself is responsible for the unique H2O2-promoted biocatalytic properties of the G4/hemin complexes. Our simulations show that once stacked atop a terminal G-quartet, hemin rotates about its vertical axis while readily sampling shifted geometries where the iron transiently contacts oxygen atoms of the adjacent G-quartet. This dynamics is not apparent from the ensemble-averaged structure. We also visualize transient interactions between the stacked hemin and the G4 loops. Finally, we investigated interactions between hemin and on-pathway folding intermediates of the parallel-stranded G4 fold. The simulations suggest that hemin drives the folding of parallel-stranded G4s from slip-stranded intermediates, acting as a G4 chaperone. Limitations of the MD simulation technique are also briefly discussed. Copy rights belong to original authors. Visit the link for more info

md islam implications limitations copy dynamics chen folding zhou g4 simulations reactivity g4s biorxiv h2o2 atomistic

Arturo Paiva: President of Globodent

The Deals for Dentists Podcast

Play Episode Listen Later Oct 18, 2020 34:44

Arturo Paiva joins us from Houston, Texas and is the president and CEO of Globodent. Go to DealsforDentists.com to find their promo offer: Purchase one BlanQuest Pro In-Office 5 Patient Whitening Treatment Kits and you get one FREE whitening syringe (3mL of 35% H2O2) with your purchase. Adding bleaching to your office is a no-brainer. It's a quick way to increase production make patients happy with their smiles and motivate them to do more cosmetic work. Arturo is going to help tell us how we can efficiently add in-office bleaching or take-home trays to quickly add some revenue to your office. Enjoy the episode!

ceo texas paiva h2o2 dealsfordentists

Stalling chromophore maturation of the fluorescent protein Venus reveals the molecular basis of the final oxidation step

Play Episode Listen Later Oct 14, 2020

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2020.10.13.337386v1?rss=1 Authors: Auhim, H., Grigorenko, B., Harris, T., Polyakov, I., Berry, C., dos Passos Gomes, G., Alabugin, I., Rizkallah, P., Nemukhin, A. V., Jones, D. Abstract: Fluorescent proteins (FPs) have revolutionised the life sciences but the mechanism of chromophore maturation is still not fully understood. Incorporation of a photo-responsive non-canonical amino acid within the chromophore stalls maturation of Venus, a yellow FP, at an intermediate stage; the crystal structure reveals the presence of O2 located above a dehydrated enolate imidazolone (I) ring, close to the strictly conserved Gly67 that occupies a twisted conformation. His148 adopts an open conformation, potentially allowing O2 access to the chromophore. Absorption spectroscopy supported by QM/MM simulations suggest that the first oxidation step involves formation of a hydroperoxyl intermediate in conjunction with dehydrogenation of the methylene bridge. A fully conjugated mature chromophore is formed through release of H2O2 upon irradiation of this intermediate, both in vitro and in vivo. The possibility of interrupting and photochemically restarting chromophore maturation, and the mechanistic insights opens up new approaches for engineering optically controlled fluorescent proteins. Copy rights belong to original authors. Visit the link for more info

harris copy protein fps o2 fp stalling absorption maturation incorporation oxidation fluorescent biorxiv h2o2 polyakov molecular basis chromophore

Changes in biochemistry and histochemical characteristics during somatic embryogenesis in Ormosia henryi Prain

Play Episode Listen Later Sep 24, 2020

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2020.09.21.307009v1?rss=1 Authors: Wu, G., Wei, X., Wang, X., Liang, X., Wei, Y. Abstract: Mature embryos were used as explant for embryogenic callus (EC) induction, and then EC further developed to form somatic embryos during somatic embryogenesis (SE) of Ormosia henryi Prain, however, some mature embryos could induced non embryogenic callus (NEC), browning callus (BC) or snowflake callus (SC). The present study was carried out to analyse the biochemical events and histochemical changes at different SE stages in 0. henryi. The contents of their energy substances, H2O2, endogenous hormones and enzyme activities were measured. The results showed that the contents of soluble sugar, starch, the activities of PPO, SOD, APX, POD, the ratios of IAA/ABA, IAA/GAs, AUX/GAs, AUX/ABA decreased gradually at different SE stages; in contrary, the contents of soluble protein, H2O2, all kinds of endogenous hormones increased gradually; however, CAT activity and the ratios of IAA/CKs, AUX/CKs, ABA/CKs, GAs/CKs increased first and then decreased. The high contents of GAs, ABA, the high ratios of ABA/CKs, GAs/CKs and the low ratios of IAA/ABA, IAA/GAs, AUX/GAs, AUX/ABA were the reasons why callus could not form EC. The low enzyme activities, the low contents of energy substances and H2O2 were related to the NEC formation; the high contents of soluble sugar, H2O2, AUX, CKs and PPO activity and the low conent of soluble protein were the basic reasons for BC formation; the high energy substances contents and the low activities of SOD, POD were easy to form SC. Copy rights belong to original authors. Visit the link for more info

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Ep 63 Own Your Health Summit Question Part 6 (The Finale)

The Panacea Concept

Play Episode Listen Later Sep 24, 2020 31:57

Join the Root Level Wellness Newsletter https://www.subscribepage.com/toxicity-quiz Name: kathleen Message: I wanted to know your protocol for healing skin cancer..potential melanoma. Treating now with Curaderm, although understand it does not work with Curaderm. The area has grown. I am alkalyzing daily with celergy juice and eat a keto diet to reduce sugar. Please advise. The lesion is on my back. Started as a raised 1/8th inch area, now 1 inch in diameter, approx... Name: Nicole Message: I have a religious exemption from immunizations for my children but am hearing they may not be accepted soon. How can I receive a medical exemption from immunizations for each of my family members? Name: Gaura Message: What are ten protocols one can follow to insure good mental health, to avoid depression? I live in a small town far from anyone I know because the rent is affordable. I am surrounded by people in a building that smoke, drink and eat animals. I rely almost exclusively for connection on the Internet, and that is not healthy either. What are your recommendations to lose 40 lbs of weight and keeping it off for a 66 yr. old male? What is the order in which one should detox all the organs like the liver, kidneys, lymphatic glands? Does eating cheese contribute to high LDL, triglycerides and low HDL? Name: Rhonda Message: Tooth abscess diagnosed 7 months ago. Did Doxy, Nystatin and Tinidazole for 10 days and stopped. Have been grape (and green juice) fasting, trying to filter kidneys, adrenal and lymph with tinctures. Continue to have left ear pain level (3) 50% of the day along with back of head and left side neck pain. Left nostril started bleeding 2 days ago. Doing chiropractic adjustments twice a month, hot and cold showers everyother day, deep breathing, vitamin C 1000-1500 mg a day, Wanted to get cold therapy done but chiro said if you have Ca (fungus) can't do because will cause it to spread. I do have parasites but not sure if CA. Have biological dentist appointment September 9 yet hesitant. Just wondering if I can have dentist pull crown of infected tooth and just let infection drain or need tooth removed? Have been watching Amanda's video and blog on teeth. Applying DMSO, H2O2/baking soda, dubh ... Thank you! Rhonda Name: Eri Message: How can we best move from cognitive thinking to sustained action? Thanks! If you don't own your Health who does? OWN YOUR HEALTH!!! Make sure to Like, Comment and Subscribe and share it with anyone who you feel it could serve. To apply for my Integrative Health Coaching program visit rootlvlwellness.com or email me at mathew@rootlvlwellness.com. For the latest promotions from Equilibrium Nutrition click my affiliate link to support the show https://track.equilibriumnutrition.com/SHU8 --- Send in a voice message: https://anchor.fm/mathew-diaz/message

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September 9, 2020

The COVID-19 LST Report

Play Episode Listen Later Sep 13, 2020 4:51

On today's episode we discuss: —Epidemiology: Public health experts affiliated with Indiana University estimated the infection fatality ratios (IFR) of SARS-CoV2 based on antibody tests in a random sample of residents aged 12+ in Indiana. The average age of decedents was 76.9 (SD 13.1). 1099 COVID-19 deaths were recorded and the average IFR was 0.26%, but stratified IFR varied by age: >60yo IFR 1.71%,

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Expression, subcellular localization, and phosphorylation of MK5 in adult cardiac ventricular fibroblasts

Play Episode Listen Later Jul 24, 2020

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2020.07.24.219790v1?rss=1 Authors: Sahadevan, P., Nawaito, S. A., Trepanier, J., Benamar, S., Sahmi, F., Theberge-Julien, G., Villeneuve, L. R., Gaestel, M., Tardif, J.-C., Allen, B. G. Abstract: MAP kinase-activated protein kinase-5 (MK5) plays an important role in cardiac fibroblast function. Although p38 MAPK and atypical MAPKs and ERK3 and ERK4 have been identified as activators of MK5, the kinases that activate MK5 remain controversial. Here we examined the expression, subcellular distribution, and regulation of MK5 in cardiac ventricular myofibroblasts and myocytes. The copy numbers for MK5 and ERK4 mRNA were comparable in myocytes and myofibroblasts, whereas that of ERK3 was much higher in myofibroblasts. Interestingly, MK5 and ERK3 immunoreactivity was detected in myofibroblasts but not myocytes whereas ERK4 immunoreactivity was detected in myocytes: treating in myocytes with a proteasome inhibitor or hypertrophic agonists failed to rescue MK5 immunoreactivity. In myofibroblasts, MK5 and ERK3 immunoreactivity was predominantly nuclear and cytosolic, respectively. In serum-starved cardiac myofibroblasts, phosphothreonine-182 MK5 (pT182-MK5) immunoreactivity was predominantly nuclear but increased in intensity and relocated to the cytoplasm in response to serum, sorbitol, angiotensin II, TGF{beta}, or H2O2 and this was prevented by inhibition of p38alpha/beta. Phos-tag SDS-PAGE revealed multiple slower migrating bands of MK5 immunoreactivity, indicating phosphorylation of MK5 at multiple sites. Phos-tag PAGE also revealed MK5 phosphorylation was increased with fibroblast activation and in hearts exposed to a chronic increase in afterload. MK5 and ERK3 co-immunoprecipitated and proximity ligation assays revealed ERK3 and MK5 in close proximity in myofibroblast cytoplasmic compartment. Furthermore, p38alpha/beta inhibition decreased the abundance of MK5 immunoreactivity in ERK3 immunoprecipitates. Finally, deleting MK5 did not reduce the abundance of ERK3 immunoreactivity. These observations suggest that p38alpha and/or p38beta are the primary mediators of T182-MK5 phosphorylation and hence MK5 activation in cardiac myofibroblasts. Copy rights belong to original authors. Visit the link for more info

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Biochemical characterization of recombinant UDP-sugar pyrophosphorylase and galactinol synthase from Brachypodium distachyon

sugar martinez copy iglesias figueroa characterization biochemical udp biorxiv recombinant h2o2 synthase

Play Episode Listen Later Jul 21, 2020

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2020.07.21.212928v1?rss=1 Authors: Minen, R. I., Martinez, M. P., Iglesias, A. A., Figueroa, C. M. Abstract: Raffinose (Raf) protects plant cells during seed desiccation and under different abiotic stress conditions. The biosynthesis of Raf starts with the production of UDP-galactose by UDP-sugar pyrophosphorylase (USPPase) and continues with the synthesis of galactinol by galactinol synthase (GolSase). Galactinol is then used by Raf synthase to produce Raf. In this work, we report the biochemical characterization of USPPase (BdiUSPPase) and GolSase 1 (BdiGolSase1) from Brachypodium distachyon. The catalytic efficiency of BdiUSPPase was similar with galactose 1-phosphate and glucose 1-phosphate, but 5-to 17-fold lower with other sugar 1-phosphates. The catalytic efficiency of BdiGolSase1 with UDP-galactose was three orders of magnitude higher than with UDP-glucose. A structural model of BdiGolSase1 allowed us to determine the residues putatively involved in the binding of substrates. Among these, we found that Cys261 lies within the putative catalytic pocket. BdiGolSase1 was inactivated by oxidation with diamide and H2O2. The activity of the diamide-oxidized enzyme was recovered by reduction with dithiothreitol or E. coli thioredoxin, suggesting that BdiGolSase1 is redox-regulated. Copy rights belong to original authors. Visit the link for more info

All The New Rules For Kissing The Blarney Stone

Dermot & Dave

Play Episode Listen Later Jul 1, 2020 10:56

Just like most other tourist attractions in Ireland, the Blarney Stone is back open. But, the tourist site which is famous for kissing is now subject to new Covid-19 rules. This morning on Dermot & Dave the lads spoke to Paul O'Sullivan, the marketing manager for the Blarney Stone, to revealed the changes that have been introduced at the iconic landmark. The new rules include: Working with Enva. An environmental company who deal with Covid solutions for a return to work. We will now be using a cleanser that is W.H.O approved and will kill 99.9% of viruses, germs, bacteria etc that may be on the stone. This has been tested on the stone to show its effectiveness in killing viruses but also that it is safe for people to come in contact with. This new spray will be used on the stone and allowed to dry for a period of 1 minute. A systematic review published in the Journal of Hospital Infection demonstrated that a (H2O2) concentration of 0.5% achieved a Log4 (99.99%) inactivation of human coronavirus HCoV229E within 1 minute. To maintain social distancing within the castle, the number of people in the building will be limited. There will be numerous hand sanitiser stations throughout the castle, before entering the stairs and also leaving, meaning people can clean their hands if they have used the handrails. A staff member at regular intervals throughout the day will spray the stairwells, handrails and rope with anti-bacterial cleanser. The person holding people kissing the stone, will be wearing a protective face shield and gloves. The gloves will be changed/cleaned after each person. Hit play on the podcast to hear more about the Blarney Stone, [audio mp3="https://media.radiocms.net/uploads/2020/07/01123330/BlarneyPodcast.mp3"][/audio]

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EP #98: Biosweep Suncoast with Jolyn & Shane Covelli

Good Neighbor Podcast

Play Episode Listen Later Jun 16, 2020 12:46

What Makes Biosweep Suncoast a Good Neighbor...At BioSweep Suncoast, our business is restoring yours!BioSweep is new to SW Florida, we opened in October 2019. We are a franchise partner of a 12-year-oldcompany founded by scientists.BioSweep offers two unique, new technology services. One is a process to permanently remove smokeand odors, the second is long-term surface decontamination.The science behind our processes has become highly relevant because of the COVID-19 virus.We know the anxiety everyone is facing to make sure we do the right things in order to re-open.We don’t make any claims about CV-19 yet, no one should be. Not enough time and testing has passed.However, we do eliminate pathogens in the Corona Spectrum family, as well as SARS, MRSA and others.Our BioSweep Base Treatment is a proprietary process utilizing UV Radiation while producing HydrogenPeroxide Vapor, Hydroxyl Radicals and Oxyradical Plasma. This is the H2O2 technology used on N95masks, but on steroids. This process eliminates all VOC’s, including viruses, bacteria and mold.This is the process we normally use for smoke and odor removal. However, we are now using it todecontaminate HVAC systems and indoor spaces. It far exceeds CDC guidelines.We work with most major insurance companies to service clients with fire/water losses, trauma andother contamination issues.Our second process is called Surface Defense. Surface Defense is a proven EPA, DOD and USDA certifiedlong-term anti-microbial. It is organic/green, sustainable and cost-effective.Our anti-microbial treatment will last up to 12 months!Surface Defense bonds to the surface at 75X the force of gravity. As it cures, millions of anti-microbial“spikes” form. They’re positively charged atoms that rupture the cell walls of microbes, killing viruses,bacteria and fungi upon contact.Normal cleaning and disinfecting are essential for every business. However, once it dries and thatsurface is touched, the contamination process starts all over again.Surface Defense is a long-term solution for wellness, prevention and peace of mind.We’re treating office buildings, restaurants, churches, apartment complexes, country clubs and more.We offer a combination of our two processes, or stand-alone, depending on our customers’ needs.Technologies like this are usually reserved for tier-1 institutions, so we are blessed to be able to bring itto the public. We feel and obligation and responsibility to do so.As a business owner of a new technology service, it’s hard to inform the public about something theycan’t see or touch, but we know that new technology is vital today and for years to come.We’ve been trying to inform our community about BioSweep and what we have to offer, but runninginto the usual firewalls and gatekeepers has been a challenge.We’re here to help our business community get back to work with some sense of normalcy.Please don’t hesitate to call or email us with any questions you may have.Stay safe and well!To learn more about Biosweep Suncoast, go to: www.biosweepsuncoast.comBiosweep Suncoast(239) 558-3110Support the show (https://goodneighborpodcast.com)

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104 KILLING MYCOPLASMA - H2O2 IV

Alternative Revelations w/Linda Emmanuel

Play Episode Listen Later Jun 6, 2020 14:53

Another way to kill mycoplasma, the most common cause of cancer and disease.Links:George Floyd articlehttp://mileswmathis.com/floyd.pdfGreat medical documentary (origin of the AMA)https://www.youtube.com/watch?v=1NiU0zJYCqkmy email address: lindamanuel.44425@gmail.com

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Parkinson Disease-Linked Parkin Mediates Redox Reactions That Lower Oxidative Stress In Mammalian Brain